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<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E776</accession>
    <name>A0A0Q3E776_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g06628v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_5g06628v3"/>
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        <property type="gene ID" value="BRADI_5g06628v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ82140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="97" mass="10239" checksum="1050DB15FC859FB4" modified="2016-01-20" version="1">MWLLGVDGVSSRGKQSHVPACCSIASTAFSSMEVVGGETSPEHSSNLVGKYSIVILGLWVFLIPGAGKGVLQTGLVLARVLIHLSKDTNRLPLNYVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E7H8</accession>
    <name>A0A0Q3E7H8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100837757</name>
        <name evidence="7" type="ORF">BRADI_5g17100v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin-like family. Class-II subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751376.1">
        <property type="nucleotide sequence ID" value="XM_014895890.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751377.1">
        <property type="nucleotide sequence ID" value="XM_014895891.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751378.1">
        <property type="nucleotide sequence ID" value="XM_014895892.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E7H8"/>
    <dbReference type="STRING" id="15368.A0A0Q3E7H8"/>
    <dbReference type="EnsemblPlants" id="KQJ83838">
        <property type="protein sequence ID" value="KQJ83838"/>
        <property type="gene ID" value="BRADI_5g17100v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83839">
        <property type="protein sequence ID" value="KQJ83839"/>
        <property type="gene ID" value="BRADI_5g17100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837757"/>
    <dbReference type="Gramene" id="KQJ83838">
        <property type="protein sequence ID" value="KQJ83838"/>
        <property type="gene ID" value="BRADI_5g17100v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83839">
        <property type="protein sequence ID" value="KQJ83839"/>
        <property type="gene ID" value="BRADI_5g17100v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837757"/>
    <dbReference type="OrthoDB" id="1668162at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3E7H8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000020">
        <property type="entry name" value="Formin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051144">
        <property type="entry name" value="Formin_homology_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733">
        <property type="entry name" value="FORMIN-J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733:SF17">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 tensin-type" evidence="5">
        <location>
            <begin position="200"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="6">
        <location>
            <begin position="792"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="446"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1170"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="542"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="602"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="635"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="654"/>
            <end position="667"/>
        </location>
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            <end position="702"/>
        </location>
    </feature>
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        <location>
            <begin position="725"/>
            <end position="747"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ83839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1197" mass="131022" checksum="2A8510AF2F8DEB59" modified="2016-01-20" version="1">MSLLSRFFYKRPPDGLLEFIDRIYVFDSCFCTEVLPHGMYPVYLNGILTVLHEEHTESSFLAINFRDGYKRSQLADILHEYNIPVIDYPRHFEGCPVLPLSLIHHFLRVCEHWLSTGNSQNIILLHCERGGWPSLAFLLSCFLIFKKLHSAENKTLDIVYREAPKGFLQLFSALNPMPSQLRYMQYVARRNISPEWPPMERALSLDCLILRAIPNFDSDNGCRPLIRIFGRNLLGKNASMTDMIFSMPKKKSLRHYRQEDCDVIKVDIQCPVQGDVVLECVHLDLDPEKEVMMFRIMFNTAFIRSNVLMLNIDDVDILWGSKERYPRNFRAEVLFCEIGGMSPPRAPTATLNGDMKGGLPIEAFSAVQELFNGVEWMENSDNAAFWLLKEFSTNSLQEKFQKLILSDMKELSKFQAKVGLQMPVMSPLDSDEEKYSVASDSISSADYEKVQHGGNSSDSENIDRALSTEDSDSIATSSVNTSSPPHGRSSSLSTEQNLLPAEPQQELPNSRQPPPSTENGHKPVSSLLEAPTAANGSKPIFSAPPPPPPPPPPPPPSGGKPVFLAPPLPPPQPPSGTKPVLLPVPPLPPPPPPPRISNASSPQPPPPPPPPLPNSQTGAPLSAPTLQPQQNVPCHPVPPPPPPPPQVHGIAKKCPPPPPPPPRPPPSSITSATAAATLSKAPPPPPPPPPGAPSPPPPPPLPAGAVQRTPPAPPPPPLMTGKKAPAPPPPPPQAPKPPGTVPPPPTLPKGTGESGPPYPPSSKIANAPAPPPLGRGRAAIGSVKGRGIGLAHQSNPPKKASLKPLHWVKVTRAMQGSLWADAQNQGNQARAPDIDLSELETLFSTAVASNASEKGGTKRGLATSKPEIVHLVDMRRANNCEIMLTKIKMPLPDMISAILALDTSVLDNDQVENLIKFCPTNEEIEMLKNYNGNKEMLGKCEQFFLELMKVPRVESKLRVFAFRIAFSTQADELRTNLTTVNDATKEVKESPKLRQIMQTILTLGNALNQGTARGSAVGFRLDSLLKLSDTRARNNKMTLMHYLCKLLAEKLPELLDFDKDLIHLEAASKIQLKVLAEEMQAINKGLEKVEQELAASVNDGAISVGFRKALKCFLDSAEAVVRSLISLYSEVGRNADSLAQYFGEDPARCPFEQVTSILVIFVNMFKKSRDENARTAEAEKKKMEKEKEKEKASISAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EPD1</accession>
    <name>A0A0Q3EPD1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">adenylate dimethylallyltransferase (ADP/ATP-dependent)</fullName>
            <ecNumber evidence="10">2.5.1.112</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_4g15770v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in cytokinin biosynthesis. Catalyzes the transfer of an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>dimethylallyl diphosphate + ADP = N(6)-(dimethylallyl)adenosine 5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36327"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:73533"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.5.1.112"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dimethylallyl diphosphate + ATP = N(6)-(dimethylallyl)adenosine 5'-triphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36331"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:73532"/>
            <dbReference type="EC" id="2.5.1.112"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.112" evidence="10"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EPD1"/>
    <dbReference type="FunCoup" id="A0A0Q3EPD1">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3EPD1"/>
    <dbReference type="EnsemblPlants" id="KQJ88140">
        <property type="protein sequence ID" value="KQJ88140"/>
        <property type="gene ID" value="BRADI_4g15770v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88140">
        <property type="protein sequence ID" value="KQJ88140"/>
        <property type="gene ID" value="BRADI_4g15770v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EPD1"/>
    <dbReference type="OrthoDB" id="775260at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009824">
        <property type="term" value="F:AMP dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052622">
        <property type="term" value="F:ATP/ADP dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.890:FF:000002">
        <property type="entry name" value="Adenylate isopentenyltransferase 5, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.890">
        <property type="entry name" value="Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF64">
        <property type="entry name" value="ADENYLATE ISOPENTENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ88140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="278" mass="30176" checksum="FEB9C81CFC796AE1" modified="2016-01-20" version="1">MEQKQQGKPKVVFVLGATATGKSKLAIAVAARFGGEVINSDKIQAYAGLPVITNKVTDEECAGVPHHLLGCVRCPDDDFTAEDFCREAGAAIGRVVSSGALPVVAEGSNHYVEALVDGGGAAFSNAHDCLFVWLDAAPEMLRRLDDMVRRGLVDEARATFDPGVADYTRGVRRAIGLPEMDAYLRHEGADDEEELLGAAVEEIKANTFGLVLEQVGKIQRMSTLEGWDVRREDCTELLAHVVAGEGVEELWRMKVWEPVEDMVRSFVDSDQSGRSLQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FJ27</accession>
    <name>A0A0Q3FJ27_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100837407</name>
        <name evidence="13" type="ORF">BRADI_3g44507v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Potassium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575043.1">
        <property type="nucleotide sequence ID" value="XM_003574995.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318366.1">
        <property type="nucleotide sequence ID" value="XM_024462598.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318367.1">
        <property type="nucleotide sequence ID" value="XM_024462599.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318368.1">
        <property type="nucleotide sequence ID" value="XM_024462600.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318369.1">
        <property type="nucleotide sequence ID" value="XM_024462601.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318370.1">
        <property type="nucleotide sequence ID" value="XM_024462602.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318371.1">
        <property type="nucleotide sequence ID" value="XM_024462603.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318372.1">
        <property type="nucleotide sequence ID" value="XM_024462604.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318373.1">
        <property type="nucleotide sequence ID" value="XM_024462605.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318374.1">
        <property type="nucleotide sequence ID" value="XM_024462606.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318375.1">
        <property type="nucleotide sequence ID" value="XM_024462607.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318376.1">
        <property type="nucleotide sequence ID" value="XM_024462608.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318377.1">
        <property type="nucleotide sequence ID" value="XM_024462609.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318378.1">
        <property type="nucleotide sequence ID" value="XM_024462610.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318379.1">
        <property type="nucleotide sequence ID" value="XM_024462611.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318380.1">
        <property type="nucleotide sequence ID" value="XM_024462612.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318381.1">
        <property type="nucleotide sequence ID" value="XM_024462613.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318382.1">
        <property type="nucleotide sequence ID" value="XM_024462614.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318383.1">
        <property type="nucleotide sequence ID" value="XM_024462615.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318384.1">
        <property type="nucleotide sequence ID" value="XM_024462616.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318385.1">
        <property type="nucleotide sequence ID" value="XM_024462617.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318386.1">
        <property type="nucleotide sequence ID" value="XM_024462618.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318387.1">
        <property type="nucleotide sequence ID" value="XM_024462619.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318388.1">
        <property type="nucleotide sequence ID" value="XM_024462620.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318389.1">
        <property type="nucleotide sequence ID" value="XM_024462621.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318390.1">
        <property type="nucleotide sequence ID" value="XM_024462622.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318391.1">
        <property type="nucleotide sequence ID" value="XM_024462623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FJ27"/>
    <dbReference type="STRING" id="15368.A0A0Q3FJ27"/>
    <dbReference type="EnsemblPlants" id="KQJ99655">
        <property type="protein sequence ID" value="KQJ99655"/>
        <property type="gene ID" value="BRADI_3g44507v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837407"/>
    <dbReference type="Gramene" id="KQJ99655">
        <property type="protein sequence ID" value="KQJ99655"/>
        <property type="gene ID" value="BRADI_3g44507v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837407"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FJ27">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00794">
        <property type="entry name" value="kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF15">
        <property type="entry name" value="POTASSIUM TRANSPORTER 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="11">
        <location>
            <begin position="42"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="12">
        <location>
            <begin position="542"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU321113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ99655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="734" mass="81362" checksum="64EF5B4A8BDED617" modified="2016-01-20" version="1">MSIEMEAPDGLVRRDSLYGEAEKVSVDKHHGSGASWSQTLHLAFQSIGVVYGDVGTSPLYVYSSTFPDGIKHQDDLLGVLSLILYTLILLPMLKYVFIVLYANDNGDGGTFALYSLISRYAKIRMIPNQQAEDAAVSNYSIEEPNSQMLRAQWVKQKLESSSSAKIALFTVTILGTAMVMGDGTLTPAISVLSAVSGIREKAPHLTQSQVVWISVGILFLLFSVQRFGTDKVGYSFAPIISVWFVLIAAIGVYNLAAHDYTVLRALNPKYIVDYFERNGKEAWVSLGGVVLCITGTEAMFADLGHFNIRAIQLSFTCILFPSVALCYMGQASYLRKFPDNVGDTFYKSIPAAMFWPTFIVAILAAIIASQAMLSGAFAILSKALSLGCFPRVEVVHTSNKYAGQVYIPEINFLIGAASIVVTIAFQTTTNIGNAYGICVVTVFSITTHLMTVVMLLIWKKHMAFIAAFYVVFGLAEFLYLSSILAKFVEGGYLPFCFSLVLMALMATWHYVRVKHYWYELDRVVPATQLTELLGRRDVGRVPGVGLLYSDLVQGIPPVFPRLVDKIPSVHAVFVFMSVKNLPIPRVPPPERFIFRRVGPAEHRMFRCVARYGYTDQIEGTKEFSAFLLERLKMFVQEEAAFSCHGDGDDDDNARRWEAQAAAEEEKRFIDAEAERGVVYLMGEANVAAAPGSSLMKKIVVNYVYTFLSKNLRESHKALSIPKDQLLKIGITYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FRB0</accession>
    <name>A0A0Q3FRB0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g57013v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FRB0"/>
    <dbReference type="EnsemblPlants" id="KQK01607">
        <property type="protein sequence ID" value="KQK01607"/>
        <property type="gene ID" value="BRADI_3g57013v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01607">
        <property type="protein sequence ID" value="KQK01607"/>
        <property type="gene ID" value="BRADI_3g57013v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FRB0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK01607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="64" mass="7735" checksum="23D5693FB06F80DB" modified="2016-01-20" version="1">MMYYLGDFNHETKLLFRNWPFLSCIITECLQESCWINFSLRQCCNSPWWSLSFSIFAYSCRSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FXL0</accession>
    <name>A0A0Q3FXL0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100839287</name>
        <name evidence="6" type="ORF">BRADI_2g12340v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565728.2">
        <property type="nucleotide sequence ID" value="XM_003565680.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FXL0"/>
    <dbReference type="EnsemblPlants" id="KQK04208">
        <property type="protein sequence ID" value="KQK04208"/>
        <property type="gene ID" value="BRADI_2g12340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839287"/>
    <dbReference type="Gramene" id="KQK04208">
        <property type="protein sequence ID" value="KQK04208"/>
        <property type="gene ID" value="BRADI_2g12340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839287"/>
    <dbReference type="OrthoDB" id="655468at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FXL0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF244">
        <property type="entry name" value="GDSL ESTERASE_LIPASE LIP-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044545908" evidence="5">
        <location>
            <begin position="36"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK04208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="378" mass="41002" checksum="3A1F339A77F065B9" modified="2016-01-20" version="1">MDRGPSRSPMSAAGLWALVLSLALMALSLPRPASAAGGFRCRPGARPVVFNFGDSNSDTGGMAAAKGWHITPPEGRAFFHHPTGRFCDGRLVIDFLCERLNITYLSPYLKAFGSNYSNGVNFAIAGSTTLPRDVLFALHVQVQEFMFFKARSLELISQGQQAPIDAEGFENALYTIDIGQNDVNALLSNLPYDQVVAKFPPILAEIKDAVQTLYFNGSRNFWIHGTGALGCLPQKLAIPRKNDSDLDQNGCLNTYNRAAVAFNAVLGSLCDQLNVQMKDATIVYTDLFAIKYDLVANHTKYGFDSPLMTCCGYGGPPYNYDLSRSCQSPNATVCADGSKFISWDGVHLTEAANAIVAAGILSSAYSKPNLKFDQFCKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G5N5</accession>
    <name>A0A0Q3G5N5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rx N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g27755v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G5N5"/>
    <dbReference type="EnsemblPlants" id="KQK06663">
        <property type="protein sequence ID" value="KQK06663"/>
        <property type="gene ID" value="BRADI_2g27755v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06663">
        <property type="protein sequence ID" value="KQK06663"/>
        <property type="gene ID" value="BRADI_2g27755v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3G5N5"/>
    <dbReference type="OrthoDB" id="683544at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377:SF50">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377">
        <property type="entry name" value="OS10G0134700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK06663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="44969" checksum="CC4E0C5D4BE5B082" modified="2016-01-20" version="1" fragment="single">MGEVANRSISFLIDKYPTPTAPVMEEKLHTLHRLLLRVRIIVEEAEGRCITNQAMICQLNILRKEMYRGYYTLDSFRTQANRADLGVSHTFALSKFNPAKRLFLSIADTNDDKLQQVVNNLSNIIVDLERVMDFLRQTKSPGTETSVDILPIVGPVHVGKSTLVSNVCNNENVRNLFSQILLVGEDDLRRRSQNANDDGHMTKQPNGALTEKEAMLIIIELPEDIDETTWRNLYSAYAGHTRRDCRSSKILDFGTVQPLVLSDLPREAFWYFFKVLTFGSADPKEQPKLSSITMELVMGMKQSFGAANMLADLLRCNLDVQFWRRTVKLARRYVQANISEFGEHPCALVWQNRPTRLVRTNIDSYMMRKQYQTSFEDEEVPKITCMDYLGAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GA65</accession>
    <name>A0A0Q3GA65_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100846714</name>
        <name evidence="8" type="ORF">BRADI_2g40550v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314660.1">
        <property type="nucleotide sequence ID" value="XM_024458892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GA65"/>
    <dbReference type="STRING" id="15368.A0A0Q3GA65"/>
    <dbReference type="EnsemblPlants" id="KQK08223">
        <property type="protein sequence ID" value="KQK08223"/>
        <property type="gene ID" value="BRADI_2g40550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846714"/>
    <dbReference type="Gramene" id="KQK08223">
        <property type="protein sequence ID" value="KQK08223"/>
        <property type="gene ID" value="BRADI_2g40550v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GA65">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000035">
        <property type="entry name" value="Leucine rich repeat protein phosphatase 2c domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="7">
        <location>
            <begin position="288"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK08223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="555" mass="62135" checksum="A1F57E7FBD808D56" modified="2016-01-20" version="1">MFFEFFEPPNLADKIHVQEWSPSILQVVTIASYLAKTLQYLQILGIVHRDIKPANILLDKDLLPHLADFGLAMYKKDIKSVSVENWKSSGKPTGGFYKKNMVGTLIYMAPEILRKDLHTEKSDVYSFAISVNELLTGVVPYTDLRAEAQAHTVLEMTYTEQQLTSAVVSQGLRPALALPESGSPPSFLSLIQRCWDPDPEQRPSFEEIIEELNVIQKHLVTCSSPSSSRLVNKSQNGNTEVHHYQEALNWFNQGELLTKKENKLDSTVNPWSAYFGQSCISIYRPTLRWGSFATCGRRETMEDTHFMLPCMSEEKDVHSFGIFDGHRGAAAAEFSVRAVPGLLRQFAHTTSPTDALAEAFVRSDMAFREELILHQKSKRITQKDWHPGCTAVTALIVRNKLFVANAGDCRAILSRAGKPHPMTRDHVASCPEERERVIKEGTEVRWQIDTWRVGAAALQVTRSIGDDDLKPAVTALPEIIETDLSAEDEFLVMASDGLWDVVSNEEVLSIIKDTVKEPGMCSKRLATEAAARGSKDNITVIVVFLRPVSTAERIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GXX1</accession>
    <name>A0A0Q3GXX1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100822176</name>
        <name evidence="2" type="ORF">BRADI_1g25330v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560114.2">
        <property type="nucleotide sequence ID" value="XM_003560066.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GXX1"/>
    <dbReference type="EnsemblPlants" id="KQK15855">
        <property type="protein sequence ID" value="KQK15855"/>
        <property type="gene ID" value="BRADI_1g25330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822176"/>
    <dbReference type="Gramene" id="KQK15855">
        <property type="protein sequence ID" value="KQK15855"/>
        <property type="gene ID" value="BRADI_1g25330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822176"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35318">
        <property type="entry name" value="BNAA10G08410D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35318:SF14">
        <property type="entry name" value="OS07G0546200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK15855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="119" mass="12448" checksum="429982621275D8F8" modified="2016-01-20" version="1">MKLFEFVRRSRRPSSAAAAPVAAVPAANASVEKKRRRPSSSSGSAAWKPTLGAISEDAAVAAARAGAEAEKAKAKLAAAAKAKARERPPPRASRPDYDDFRRFGAPTVLPAFGPAAFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H090</accession>
    <name>A0A0Q3H090_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100826366</name>
        <name evidence="13" type="ORF">BRADI_4g07017v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Tice H."/>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ81744"/>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
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                <person name="Schmutz J."/>
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                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK07284"/>
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        <property type="gene ID" value="BRADI_2g34545v3"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK07284.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="97" mass="9880" checksum="83AB3B248CDAE70B" modified="2018-03-28" version="2">MMGLQVAGDPIAAAARSCGSSMAAALGLGSSDGSGRGPTATATTGSLSFFALIVCNSFNFVDLIQFFGGFVTLCWGIFDELCVSAVVLIRFLDGALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JYP7</accession>
    <name>A0A0Q3JYP7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g68094v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JYP7"/>
    <dbReference type="EnsemblPlants" id="KQK22571">
        <property type="protein sequence ID" value="KQK22571"/>
        <property type="gene ID" value="BRADI_1g68094v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22571">
        <property type="protein sequence ID" value="KQK22571"/>
        <property type="gene ID" value="BRADI_1g68094v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JYP7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK22571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="38" mass="4278" checksum="B8E415CEBF1AE8AE" modified="2016-01-20" version="1">MVGLKLDPGNAEMDKALREAVEEMKKDHFARKSYKPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K1J0</accession>
    <name>A0A0Q3K1J0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g75601v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K1J0"/>
    <dbReference type="STRING" id="15368.A0A0Q3K1J0"/>
    <dbReference type="EnsemblPlants" id="KQK23715">
        <property type="protein sequence ID" value="KQK23715"/>
        <property type="gene ID" value="BRADI_1g75601v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK23715">
        <property type="protein sequence ID" value="KQK23715"/>
        <property type="gene ID" value="BRADI_1g75601v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K1J0"/>
    <dbReference type="OrthoDB" id="693750at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053253">
        <property type="entry name" value="Sex_diff_modulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087:SF21">
        <property type="entry name" value="OS03G0782100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087">
        <property type="entry name" value="OS07G0539200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="382"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK23715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="497" mass="54488" checksum="C6158C57D987D2A3" modified="2016-01-20" version="1" fragment="single">ARVRAKAASAVVSAAPNVNNIDEESQGRTLVSPRHRRGPAGLPRSRPGNYAGQHRAGEALNAAGHGYGYKKRFRGECFRCLQPGHRKAECRDPLWCTACKLVGHLARQCPSKRRTASGSGNMGSRGLSSLGAARSGDRPGEAHRRPLEDFAVITSSPAMGEMTGMLRATAMVITAHAGFAHLSNVAIGRALVEDLHLRPEVVARDLARDAGVFTFQGVPFTLSPWTPTQDGTHYQWKYYCRLAVEKLPLQAWNVRALQELPPGKCLVDCIEEQSESLHNAEFCFVWVWAFNPDRLPRVLYTDIVDTIAEPRARHGQLVGHPRDDGREGPKFRVLVHLDTTKDFSPLEPLSPVSVAFGLPSGSLEDFRPWPVVERFSYTKHLVNGIEPPPPPRASVHSRLGAGRRDEDADRGGDGRGSSRRRFRSWREALLRGRDYNARADEAVAPTRSRATTPVKQTTVKIARMSRCGADTEMPLVTVVGPSCELTLGPPRSSSGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KKN3</accession>
    <name>A0A0Q3KKN3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">[histone H3]-lysine(27) N-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.369</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100834709</name>
        <name evidence="15" type="ORF">BRADI_2g61717v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysyl(27)-[histone H3] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl(27)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60296"/>
            <dbReference type="Rhea" id="RHEA-COMP:15544"/>
            <dbReference type="Rhea" id="RHEA-COMP:15548"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="EC" id="2.1.1.369"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.369" evidence="11"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KKN3"/>
    <dbReference type="FunCoup" id="A0A0Q3KKN3">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3KKN3"/>
    <dbReference type="EnsemblPlants" id="KQK11697">
        <property type="protein sequence ID" value="KQK11697"/>
        <property type="gene ID" value="BRADI_2g61717v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11697">
        <property type="protein sequence ID" value="KQK11697"/>
        <property type="gene ID" value="BRADI_2g61717v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="336088at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KKN3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140953">
        <property type="term" value="F:histone H3K27 monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
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    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
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        <location>
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        </location>
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    <evidence type="ECO:0000256" key="2">
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        </source>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="KQK11697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="346" mass="39102" checksum="612A3E5287AF2C8F" modified="2016-01-20" version="1">MADVMRRSVPVDAPPPVARARGGRAYETLPCEVCGSGDRDEDMLLCDLCDRGRHTFCLRPILAAVPLGYWLCPDCAPPSKPVIGFPMKQKKIVEFFRIQKGAQDVEAPKCAHPQDARRRRKRSLVMQKKRRKILPFVPTEKRVQRLRQMASAATALTSSKMEFSNELTYVRSMAPKSSNQAGLEEGGMQVLSKEDKETIELCRAMQKSGQCPPLLVVFDSHEGFTVQADADIKDMTFIAEYTGDVDYFENRVNDDCDSIMTLLWTTDPSKRLVICPDKRGNIARFINGINNHTPDGKKKLNIKCVRYNIDGESHVLLVACRDIACGERLYYDYNGHEYAYPTHDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3L1Q9</accession>
    <name>A0A0Q3L1Q9_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g32939v3</name>
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    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
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    </dbReference>
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            <end position="87"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK17185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="87" mass="9937" checksum="5390954081B462CF" modified="2016-01-20" version="1">MDEQGPKDGGCDFWYWIDEYAGFLCETGTVPQESPMLIPTGWVVVDAGMAEDNHSRLIQDASERCRGRGKSSFSKKRKEKKRMHLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LDD1</accession>
    <name>A0A0Q3LDD1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
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    <gene>
        <name evidence="3" type="ORF">BRADI_4g32548v3</name>
    </gene>
    <organism evidence="3">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LDD1"/>
    <dbReference type="FunCoup" id="A0A0Q3LDD1">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3LDD1"/>
    <dbReference type="EnsemblPlants" id="KQJ90573">
        <property type="protein sequence ID" value="KQJ90573"/>
        <property type="gene ID" value="BRADI_4g32548v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ90573">
        <property type="protein sequence ID" value="KQJ90573"/>
        <property type="gene ID" value="BRADI_4g32548v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LDD1"/>
    <dbReference type="OrthoDB" id="686198at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072:SF1">
        <property type="entry name" value="OS09G0122200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ90573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="261" mass="30142" checksum="8242FE236CCA0520" modified="2016-01-20" version="1">MKLFLIQLLKDHDVPGFRNQNAWSKEAWTSITSQLNTKFVVSFTVNQIKQKEQDLKKDYRSVKDLLAESGSGWDSVRMMVDAPADIWATFAARKNSKDALQWRDKSFPYYDELVPLYDGRHAEGRTRRGMDYYASRANNVRSSTPFQQMKSTPSSAQARTEKPSSRRAKKQKRSTTEPPEGFHERYLKLKRKEIDRFAAIEEKKASIEEKKAEDPYSINKCIITLEGLDGLEVSDILLASDIFQPKDNREVFLSYLHCVCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3M129</accession>
    <name>A0A0Q3M129_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Neprosin PEP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g35614v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3M129"/>
    <dbReference type="FunCoup" id="A0A0Q3M129">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3M129"/>
    <dbReference type="EnsemblPlants" id="KQJ98229">
        <property type="protein sequence ID" value="KQJ98229"/>
        <property type="gene ID" value="BRADI_3g35614v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ98229">
        <property type="protein sequence ID" value="KQJ98229"/>
        <property type="gene ID" value="BRADI_3g35614v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3M129"/>
    <dbReference type="OrthoDB" id="777472at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1320.10">
        <property type="entry name" value="Outer-capsid protein sigma 3, large lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004314">
        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025521">
        <property type="entry name" value="Neprosin_propep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF237">
        <property type="entry name" value="OS08G0411100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03080">
        <property type="entry name" value="Neprosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14365">
        <property type="entry name" value="Neprosin_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52045">
        <property type="entry name" value="NEPROSIN_PEP_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neprosin PEP catalytic" evidence="1">
        <location>
            <begin position="182"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ98229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="433" mass="46929" checksum="72401FDC501D97AA" modified="2016-01-20" version="1" fragment="single">TKHKLNLQVGPVCQVQRPVQIAVLRSSNQSASAVPSRSGRDSITFDLLFLAAVQVGGGLLQFTAAVRTMVSRNSVLSQDDFTLEAELKMLNKPYVKSFKDNYGVVFDCVDIHKQPAFDHPLLKNHKLQIPPSSSAGTGLGLPTTSGESCPYGTVPIRRTLKEDLVRRRASSQVMKPQTEYTSTIEGQHFAQVLFDSEKGSLFVDVKADIDVYPLVIPPGQKSTAQILVVDRRPSSATVVQAGWHIDSQHEGDNQPRFMVYWTADGYQKTGCLNYECPGFVVTSQATTPGMALPVGKITLKILRVIHDIHHISLNNFIFDFQTGNWNVYLNGEMVGYFPGAIVNGMDGSTQIQMGGTVYSTPGEGKSPPMGNGILAADTNAAAKFTWVVMRGSKTINYMVAKDIDSSIYDAVVTSASEDGPQGFAFVYGGPGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NPU2</accession>
    <name>A0A0Q3NPU2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100835311</name>
        <name evidence="3" type="ORF">BRADI_1g49107v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564314.2">
        <property type="nucleotide sequence ID" value="XM_003564266.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NPU2"/>
    <dbReference type="STRING" id="15368.A0A0Q3NPU2"/>
    <dbReference type="EnsemblPlants" id="KQK19574">
        <property type="protein sequence ID" value="KQK19574"/>
        <property type="gene ID" value="BRADI_1g49107v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835311"/>
    <dbReference type="Gramene" id="KQK19574">
        <property type="protein sequence ID" value="KQK19574"/>
        <property type="gene ID" value="BRADI_1g49107v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835311"/>
    <dbReference type="OrthoDB" id="273452at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3NPU2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF11">
        <property type="entry name" value="LIPASE YOR059C ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="2">
        <location>
            <begin position="75"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK19574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="469" mass="51252" checksum="92EEDC90DBF9171B" modified="2016-01-20" version="1">MASALCSPPCHRSHRSFHSSLLRPSAPVCHACHRSRTGGPPGFNTRAYPYSRPISTSRGGPVSYSSMDQSKSKQGPDHLLILVHGIMASPSDWTYGEAVLKRQLGGKFFIHASSSNIYTKSFDGIDVAGRRLANEVLDVVQKMAGLRKISFIAHSLGGLFARYAISILYSLQTKETGLGSAYVMPTVGGSEIPQHTSGLGAIAGLEPINFITLATPHLGVRGKNQLPFLQGLSFLEKLAAPLAPFIVGRTGGQLFLTDGEPSKPPLLLLMTSDHEDKKFISALAAFKNRVLYANVSYDHMVGWRTSSIRRELDLKTPLHRSVDGYKYIVNVEYCSAVSSDGPHFPSRAARAKEAAQSTPNIENTEEYHEMMEEEMIHGLQRVGWKKVDVNFHASLWPYSAHNSIHVKNEWLHNAGAGVIAHLANSIKQSCFHANLSTGVSGTLDCRRPELFGTARSQLPARLKMGRNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PF33</accession>
    <name>A0A0Q3PF33_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF629 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100845321</name>
        <name evidence="3" type="ORF">BRADI_4g14560v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758809.1">
        <property type="nucleotide sequence ID" value="XM_014903323.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PF33"/>
    <dbReference type="FunCoup" id="A0A0Q3PF33">
        <property type="interactions" value="1543"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ87953">
        <property type="protein sequence ID" value="KQJ87953"/>
        <property type="gene ID" value="BRADI_4g14560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845321"/>
    <dbReference type="Gramene" id="KQJ87953">
        <property type="protein sequence ID" value="KQJ87953"/>
        <property type="gene ID" value="BRADI_4g14560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845321"/>
    <dbReference type="OrthoDB" id="679732at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006865">
        <property type="entry name" value="DUF629"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34465">
        <property type="entry name" value="CARBOXYL-TERMINAL HYDROLASE-LIKE PROTEIN, PUTATIVE (DUF627 AND DUF629)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34465:SF3">
        <property type="entry name" value="OS09G0547900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04780">
        <property type="entry name" value="DUF629"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF629" evidence="2">
        <location>
            <begin position="328"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="652"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ87953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1074" mass="121628" checksum="931F376D20CF481D" modified="2016-01-20" version="1">MVEPAASAGVAALRKEAEAVRALDRDGHHDEAHARLDELVDRHAGSPLVLHLAGLFHHAAARRAKDEKDLAAQHLVSAELYVTLAKRFAPNCIEISALLARVLFEASKHEEAEAEIRRAIEIPWPVDPAENNVVYDEERCANTTRDQRVENSREMATLFFKVIGDWVCNNYVPAVVGKVLEVELDADDREGAAQALKSANDLAKRYPYSSRAQLFRAYMKLNFARGLDATMDRRPFLDRIRRNMNEAANLFESSLVLAIFRAKLCFVLGLYGAAYLECVRGFNTGDPVDPKLEDVPPGSVDGEECADRLSSIYTEFARLIRRVVWVVKGSWDSMTSAQQDSFLSVRLFELRKYYDDVYEDDHWAARTISDAMTFVKKTRSWRFWICPYCVGKKLPDTDSLLRHMCSKHPAEKDLPKLRTVLEPKGTSVDDNSLDEITVGQDSEDHYFFHFKKKDYIFEHLFLKPSTVSDEESFAKIREEKIKEGTEILEKIKQRLRNLPTDNLSAEFYKARTEIQDLWHDFLAISLLDYRVVILPLVKSFLWEELIKFTSDDKTASKSISNADIDTVFPSVLYTPNMDAMLELTYKLSDGNKDHESGEDQETVNIKDEFIKSTSEDNAASKSIDNADIDTVFPNVVYASGSNAILEHTYKLSNGNKDHESGEDQETENMKPSGLDKTLVDDEKGEERCSEDLLEDRNSETLIDKKLSDPTIYMDGSGKSAARIATLERNKKGTSGQSVGKMASNFLYQPSVNIFNHKNAEKVLSSLRVIIQSLCNLKHLRDKFLMGELKWDPSSNNPCIADLLYEIFFAWERYEPYPTVDVLTSVKTILLRLADDSSIYEKVGEIFASETVVTILIGLHMSDTCSSFSFNTEIGGNVVNPITCGACICPTHNLFGINFNVQMSCRCGKCSDKYLYTTLFHILDAGSAQTTKIKSFSELQFLLDEQFCEDHSCKNCGIIENVDLFLSNMPQFFTIVLNWASGSRSQDTFSEVLAGITSPLDADFFCRSAHSATKYVVTSMICYADERYLCFARDEDSWLIFDSDKITTVFTWDHLLESFKDCKLQPEVLFFEVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PVE3</accession>
    <name>A0A0Q3PVE3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g04315v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93401.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PVE3"/>
    <dbReference type="EnsemblPlants" id="KQJ93401">
        <property type="protein sequence ID" value="KQJ93401"/>
        <property type="gene ID" value="BRADI_3g04315v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93401">
        <property type="protein sequence ID" value="KQJ93401"/>
        <property type="gene ID" value="BRADI_3g04315v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3PVE3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ93401.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="76" mass="8365" checksum="7ABCB692BD0D9361" modified="2018-03-28" version="2">MSTSSSYSYKAILLGIVCLLLTLGFLSLAPPFPTASSNNTRKSRYIVITGCNNYCNVACCYCNIHRFPPVCEKCCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QPY9</accession>
    <name>A0A0Q3QPY9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil SMC6 And NSE5 INteracting (CANIN) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100821696</name>
        <name evidence="2" type="ORF">BRADI_2g08380v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230680.1">
        <property type="nucleotide sequence ID" value="XM_010232378.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230681.1">
        <property type="nucleotide sequence ID" value="XM_010232379.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3QPY9"/>
    <dbReference type="STRING" id="15368.A0A0Q3QPY9"/>
    <dbReference type="EnsemblPlants" id="KQK03522">
        <property type="protein sequence ID" value="KQK03522"/>
        <property type="gene ID" value="BRADI_2g08380v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK03523">
        <property type="protein sequence ID" value="KQK03523"/>
        <property type="gene ID" value="BRADI_2g08380v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK03524">
        <property type="protein sequence ID" value="KQK03524"/>
        <property type="gene ID" value="BRADI_2g08380v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821696"/>
    <dbReference type="Gramene" id="KQK03522">
        <property type="protein sequence ID" value="KQK03522"/>
        <property type="gene ID" value="BRADI_2g08380v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03523">
        <property type="protein sequence ID" value="KQK03523"/>
        <property type="gene ID" value="BRADI_2g08380v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03524">
        <property type="protein sequence ID" value="KQK03524"/>
        <property type="gene ID" value="BRADI_2g08380v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821696"/>
    <dbReference type="OrthoDB" id="674980at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3QPY9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37212">
        <property type="entry name" value="ACTIN PROTEIN 2/3 COMPLEX SUBUNIT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37212:SF2">
        <property type="entry name" value="ACTIN PROTEIN 2_3 COMPLEX SUBUNIT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="52953" checksum="532719C4D7CAD496" modified="2016-01-20" version="1">MVIDDPLDFEGENDPLLSAPRPAKRKKVIGLDDLLVDFFESGKDELKASGSKSKRGLKGYNSDDEDKKIREKEINFCKFVEECEEQAKAMDPGDDVPQWGLQVFGCQKLPPILNDSGIENCQLLQSFSGEEHLGFDPEMEQGEGFLEGMLRDGWLLKLVLLYGSVEDSIVSWTLTKLLYSTNKKLQNSASEFWDSLLSLNEDDKQLIKFGYFPSYSILKDAVLNYGYLHDAPFTKTCISGSAVADDGPPQNIIAWLRVVSACCKIRKMHSVFSPSEAEDLLVIVISLFLDRHLEGLLLILGDCLNSLVLYFNSSEWESSCLVVAESIAKRVPLDLNCLRIVDCITGTNERSKFLRSQLALQFLKISFGLKVANVEKILKSVTSINVKEKECNFFRLYVYLVLMDNLLFSSDAFRNKPAIIEAWRNYLRNCSTQIGCTDWRFYASKVRNKASYLLQGAILKRPAVSGISAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R2U1</accession>
    <name>A0A0Q3R2U1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g37672v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R2U1"/>
    <dbReference type="EnsemblPlants" id="KQK07794">
        <property type="protein sequence ID" value="KQK07794"/>
        <property type="gene ID" value="BRADI_2g37672v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07794">
        <property type="protein sequence ID" value="KQK07794"/>
        <property type="gene ID" value="BRADI_2g37672v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3R2U1"/>
    <dbReference type="OrthoDB" id="682975at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK07794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16836" checksum="148F90171EAB0F44" modified="2016-01-20" version="1">MGIPSSKNGIHLEDIHDHTCLFCAELETTQHLFFDCLVARILWSHLSEMFNVTLGSNLEHTTSFWGHNKRFEVINMTTAGVLWSLWKCQNNLFFRSSSWSSMQVLCHVALRHLRSWTSLCSSAGQGALEHILQQLEGKSVEIPRLLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R706</accession>
    <name>A0A0Q3R706_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100829184</name>
        <name evidence="13" type="ORF">BRADI_2g46340v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232133.1">
        <property type="nucleotide sequence ID" value="XM_010233831.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232134.1">
        <property type="nucleotide sequence ID" value="XM_010233832.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R706"/>
    <dbReference type="STRING" id="15368.A0A0Q3R706"/>
    <dbReference type="EnsemblPlants" id="KQK09154">
        <property type="protein sequence ID" value="KQK09154"/>
        <property type="gene ID" value="BRADI_2g46340v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09155">
        <property type="protein sequence ID" value="KQK09155"/>
        <property type="gene ID" value="BRADI_2g46340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829184"/>
    <dbReference type="Gramene" id="KQK09154">
        <property type="protein sequence ID" value="KQK09154"/>
        <property type="gene ID" value="BRADI_2g46340v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09155">
        <property type="protein sequence ID" value="KQK09155"/>
        <property type="gene ID" value="BRADI_2g46340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829184"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3R706">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055164">
        <property type="entry name" value="EDR1/CTR1/ARMC3-like_pept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF821">
        <property type="entry name" value="ATP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14381">
        <property type="entry name" value="EDR1_CTR1_ARMC3_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="554"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK09154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="810" mass="90164" checksum="AECF594873654808" modified="2016-01-20" version="1">MRDYTVRAMDEAPTSSGQSEATSCEPSWWPPDLLDKIESVAISRKQEVLVDKESRIILANSRSSSWKASQLLWSTGTYSGFIPNGFYSIIPDKKLKECFPTIPSLNELQTLEADGLRTDIIVVDAEKDKKIFMLKQLSAALVKGLNSSPALVIKKIAGLVFDCFKGQNPDVSPGRAATEDTHFFGNKGPQLLGQIRHGSCRPRAILFKVLADAVGLESKLVVGLPDDGADGFVDSYKHMSVVVPLNSMELLVDLMRFPGQLIPFSAKAIFTSHISAAGESDSAENDSCDSPLEPNSPLYGLSDKVEAEGIEASSNLSGRSLRNTMLRSRTFSEGKLSTSCSEPNIANAFWRRSQRKGVAEEPRCASSSPEHPLMRAKGRSILSGERQSFQEYTDGVTSRSDDPGGTTIPTPRRIRRRSISITPEIGDDIVRAVRAMNETLKQNRLQRDHVNDGSCSYISEDTSNAHDCPNKDDKSGRDVATNNGLRNRTCSTQKAMSLPTSPHDYEGGISETSDNCDFISKEKMVFAWNKVLQSSPFNKPLLPFQEWNIDFSELTIGTRVGIGFFGEVFRGIWNGTDVAIKVFLEQDLTTENMEDFCNEIYILSRLRHPNVILFLGACMVPPHLSMVTEYMEMGSLYYLIHMSGQKKKLSWRRRLKIIRDICRGLMCIHRMKIVHRDLKSANCLVNKHWTVKICDFGLSRAMTDSPMTDNSSAGTPEWMAPELIRNEPFSEKCDIFSLGVIMWELCTLSRPWDGISPVQVVYAVANEGSRLEIPEGPLGKLIADCWAEPQDRPSCQEILTRLLDCEYSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RAF9</accession>
    <name>A0A0Q3RAF9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g51696v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RAF9"/>
    <dbReference type="EnsemblPlants" id="KQK10050">
        <property type="protein sequence ID" value="KQK10050"/>
        <property type="gene ID" value="BRADI_2g51696v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10050">
        <property type="protein sequence ID" value="KQK10050"/>
        <property type="gene ID" value="BRADI_2g51696v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3RAF9"/>
    <dbReference type="OrthoDB" id="661559at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33181">
        <property type="entry name" value="OS01G0778500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33181:SF17">
        <property type="entry name" value="OS01G0778500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK10050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="69" mass="8064" checksum="11EBB9D992899B8A" modified="2016-01-20" version="1">MGWLRSLVSPLRKLWCQMNTVQRKKRGIYILYDDVKSCQCEDVHVLWSILVESHGLPPPTPTTVLRLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RM12</accession>
    <name>A0A0Q3RM12_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100822380</name>
        <name evidence="2" type="ORF">BRADI_1g13516v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g13516v3"/>
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    </dbReference>
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        </location>
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            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK13946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="442" mass="50808" checksum="037B888E2E4D474F" modified="2016-01-20" version="1">MEQFAGADLLPDDLLADVLGRLEPRWLAASRCVCKDWRAIIDARRLLRADLLPLSLASIIISFDMLDLSDFFDRPSSTGRPAISARIHDCLPSTAEKSPVRDHCNGLLLLHNGWVVNPATRWAVLLPPCPARCTTTEADFYHRYLVYDPTVSTHFEVFRVPGFYYYHRHVDHVLEESEWPPSLYTMHVYSSRSGHWEERSFVREGDAAGTVSDSRLHGRKCYAVCWQGALYVDCLNHFLRISLSNSKYQVIKPPVGLGSGWAVYQEKYLGKSEKGVYYASLGDNSFLSIWILTESSNQMEWVLKLNNLDTRSLNCYSQDHGPWVSQDINYESYFPNDEKKAQVEENFEWSSDDDDALNNENRVKKYDPAGIGILGFHPRKEIIFLSKSVETGYAYHLKSSRIQTLGSIYPTRYQDILLPHEHNIRESFPYTPCWMRGFPEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RQ75</accession>
    <name>A0A0Q3RQ75_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g21087v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15162.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RQ75"/>
    <dbReference type="EnsemblPlants" id="KQK15162">
        <property type="protein sequence ID" value="KQK15162"/>
        <property type="gene ID" value="BRADI_1g21087v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15162">
        <property type="protein sequence ID" value="KQK15162"/>
        <property type="gene ID" value="BRADI_1g21087v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3RQ75"/>
    <dbReference type="OrthoDB" id="624443at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="3">
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            <begin position="157"/>
            <end position="381"/>
        </location>
    </feature>
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            <end position="442"/>
        </location>
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            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK15162.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="53163" checksum="1BAD11C0F19ED8D9" modified="2018-03-28" version="2">MAASLSGAPFTNVDPETAKYMRSKGLDPHELDRSMENILSMIYGNLPRRPSPPPPCSPPASAAAAAGLPDALLRDIVSRLPVKDAARTAALAARWRGVWRSAPLVLADVFLLSDGPNLPPSADTRAAVSRVFASHPGPFRCVHLLCSRMGGAHQAELTRWLRLLAARGVQELVLVNRPWPREVPLPTTLFTISTLTRLYIGVWKFPDVSGLRGVSFPNLRELGICSVELKDGDIDAVVARSPALEILNIAGNFKAVNLRLVSNSLRCVQVCASVMKSIAVVNAPRLERLILGNAVHPDGALCTRLKIRNAPKLRLFGYLEPGKHALEINDTVIMAGVEVRASMMLTTVKVLGLRVHFGVQNDINMVATFLRCFPNVEALHIASEKCDQPTGKLNLKFWEDQAGPIVSVLLRIGVMTFSAFRGEQYELSFLQYFFESARMLKTKWATNFDLAVMGRMIPREEHSGPFSREQILVMETLLHQLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CH49</accession>
    <name>A0A2K2CH49_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g14235v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CH49"/>
    <dbReference type="EnsemblPlants" id="PNT61356">
        <property type="protein sequence ID" value="PNT61356"/>
        <property type="gene ID" value="BRADI_5g14235v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61356">
        <property type="protein sequence ID" value="PNT61356"/>
        <property type="gene ID" value="BRADI_5g14235v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CH49"/>
    <dbReference type="OrthoDB" id="1721816at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT61356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="51" mass="5625" checksum="7D60D0BA6687A88B" modified="2018-03-28" version="1">MLDRDASSRITADGVLSHPWVLFYTECTLKAVTANLCVTKKIGPQFSMGHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CL78</accession>
    <name>A0A2K2CL78_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104584872</name>
        <name evidence="3" type="ORF">BRADI_4g08160v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238955.1">
        <property type="nucleotide sequence ID" value="XM_010240653.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CL78"/>
    <dbReference type="EnsemblPlants" id="PNT62785">
        <property type="protein sequence ID" value="PNT62785"/>
        <property type="gene ID" value="BRADI_4g08160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584872"/>
    <dbReference type="Gramene" id="PNT62785">
        <property type="protein sequence ID" value="PNT62785"/>
        <property type="gene ID" value="BRADI_4g08160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584872"/>
    <dbReference type="OrthoDB" id="669902at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CL78">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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            <end position="111"/>
        </location>
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        <location>
            <begin position="75"/>
            <end position="111"/>
        </location>
    </feature>
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        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT62785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="11993" checksum="9AE52B6552926525" modified="2018-03-28" version="1">MAMRIWVVVTAMVCVVATAAMADMEEGDVGEYWQKRTAETRFKHGGPLHDLVSAATRYHQDLLGHRDGRRYLLAEEEAASAPTPPQAQAPASADGKPAGEHNTLADHDIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
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        <name type="synonym">Trachynia distachya</name>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="PNT62983"/>
        <property type="gene ID" value="BRADI_4g10213v3"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="PNT62983.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CNA4</accession>
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            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT63496">
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    </dbReference>
    <dbReference type="Gramene" id="PNT63496">
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        <property type="gene ID" value="BRADI_4g16635v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="447251at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="76" mass="8818" checksum="7439EF015A180431" modified="2018-03-28" version="1">MCPDATDIYGYMHILYAKYVLSSIIASCSFYVQVADTSRARVHRSTRKRTRNGSIHAFLTLYPLETERLSKNRKNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CPH9</accession>
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            <fullName evidence="7">NAC domain-containing protein</fullName>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="PNT63913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A2K2CPH9"/>
    <dbReference type="EnsemblPlants" id="PNT63913">
        <property type="protein sequence ID" value="PNT63913"/>
        <property type="gene ID" value="BRADI_4g22216v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63913">
        <property type="protein sequence ID" value="PNT63913"/>
        <property type="gene ID" value="BRADI_4g22216v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CPH9"/>
    <dbReference type="OrthoDB" id="676820at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF130">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="13"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT63913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="172" mass="18971" checksum="5989284F814042AC" modified="2018-03-28" version="1">MAMPEARGGGLGLPTGFRFMPTDEELTASYLRKKALSFPAPSEIIPIADLARPHPPSIPPAYDKMIQFRDETHGVKFFFSRSTLVFFTVNVGSGATRRTQWIMHEYRLHPALLAVVAKVGKDVEDSVVCRFFKKATRRGGNPEDMGGPSSPHLSCVTEELGDDEEEMASSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CR27</accession>
    <name>A0A2K2CR27_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g29283v3</name>
    </gene>
    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
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    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        <location>
            <begin position="97"/>
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    </feature>
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            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
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</entry>
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            <strain evidence="1 2">Bd21</strain>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT64562"/>
        <property type="gene ID" value="BRADI_4g30235v3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="PNT64562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="86" mass="9527" checksum="2BB4E7C4F640E1AB" modified="2018-03-28" version="1">MGSGYDKISYPSTGTSGTVPELVGCHPDRWGSVHQRASLVDTNVILTFMWKEKLDASLPLDLPRQQHKKGSTTAIIAVTRKDTHYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CS48</accession>
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT64833">
        <property type="protein sequence ID" value="PNT64833"/>
        <property type="gene ID" value="BRADI_4g33722v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64833">
        <property type="protein sequence ID" value="PNT64833"/>
        <property type="gene ID" value="BRADI_4g33722v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CS48"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT64833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="68" mass="7602" checksum="CBB79F145075525D" modified="2018-03-28" version="1">MVQLKNFSWTRRKVPRKRSCTAMVHGSWHGAVSNDLRLLAEPLRPLFVSWSAATLGSSHACSFLLGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CUN0</accession>
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        <recommendedName>
            <fullName evidence="7">DYW domain-containing protein</fullName>
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    </protein>
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        <name evidence="5" type="primary">LOC112268507</name>
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT65729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317701.1">
        <property type="nucleotide sequence ID" value="XM_024461933.1"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT65729">
        <property type="protein sequence ID" value="PNT65729"/>
        <property type="gene ID" value="BRADI_3g01489v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112268507"/>
    <dbReference type="Gramene" id="PNT65729">
        <property type="protein sequence ID" value="PNT65729"/>
        <property type="gene ID" value="BRADI_3g01489v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:112268507"/>
    <dbReference type="OrthoDB" id="726582at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CUN0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
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    </dbReference>
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        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
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    </dbReference>
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        <property type="entry name" value="PPR_rpt"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF497">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="200"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="336"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT65729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="57974" checksum="4E25E5C621DC613E" modified="2018-03-28" version="1">MNRSICHHLLAHCCKSIRELEQIHAGALVHGLHPGNQSVSCKMFRSYADLGRAADARRLFGEIPEPDLVSLTSLMSLHLQLNQHREAMWLFSRVVASGVHRPDGFAVVGALSASGGVGDLRAGEALHGMIFRCGLGSEPVVGNALVDMYSRCGRFEGAVAVFEEMAVKDHVTWGSMLHGYIKCVGVDSALSFFDQMPMKSVVSWTALITGHVQAKNPIRALELFGRMVLEGHRPTHFTIVGVLSACADIGALDLGRVIHGYGSKFNVSTNIIVSNALMDMYAKSGSIEMAFAVFEEVPLKDAFTWTTMISSFTVQGDGSKALELFGDMLRSGIVPNSVTFVSVLSACSHAGLIQQGKELFDKMRGTYNINPQLEHYGCMVDLLGRGGLLEEAEALIYDMDVEPDVVIWRSLLSACLVHGNDRLAEIAGKEIIKREPGDDGVYVLLWNIYASSDKRKEALDMRNQMLMRKVFKKPGCSWIEVDGGVHEFLVEDKTHAARREIYETLNEINRHLKMDLRVFADGELLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CUX7</accession>
    <name>A0A2K2CUX7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g03242v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CUX7"/>
    <dbReference type="FunCoup" id="A0A2K2CUX7">
        <property type="interactions" value="657"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CUX7"/>
    <dbReference type="EnsemblPlants" id="PNT65829">
        <property type="protein sequence ID" value="PNT65829"/>
        <property type="gene ID" value="BRADI_3g03242v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65829">
        <property type="protein sequence ID" value="PNT65829"/>
        <property type="gene ID" value="BRADI_3g03242v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CUX7"/>
    <dbReference type="OrthoDB" id="655770at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31264:SF22">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31264">
        <property type="entry name" value="OS07G0554500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT65829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="42967" checksum="6F8E529E82D80BC4" modified="2018-03-28" version="1">MAPPAPHIIDEILEDIFLRLPTPAALARVSTASPSFRRIITERSFLRRFRKLHPPPLLGFATNNGVFEPVQDPDPSAPLARALADAADFTYSSSSPGPEMIRLLLGLPAISAMAADAVFTNLAVCDPLSRKYVLLPPIPKDMTVQTEPLVEYGRFLASAGEDEDEDETLFKVICTAHYKSKLVVFVFTSSSGQWCKVASPSWSFLGPIGFRWNSLGHPYYLRGCFYWMPLSTDKLLVLDTCRMESSTVNNLPGYREQLMNQPGRVMYTPSIVIGTEGALEMITLVGDLGASGLFYLYHTSQENKGESCNQCQLKNVIALPSGWTYNIVGATEGLMFIRGMEARSRDPDINLYSLDVKTSEVKKVCRATFYGHYCSYFGFPPSLSKPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CV88</accession>
    <name>A0A2K2CV88_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g04617v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CV88"/>
    <dbReference type="EnsemblPlants" id="PNT65935">
        <property type="protein sequence ID" value="PNT65935"/>
        <property type="gene ID" value="BRADI_3g04617v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65935">
        <property type="protein sequence ID" value="PNT65935"/>
        <property type="gene ID" value="BRADI_3g04617v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CV88"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036043301" description="Secreted protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT65935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="7904" checksum="7D1E14B636679C7F" modified="2018-03-28" version="1">MILWSFTTSDMWSSVVSHLMAFSSLAWPAACAVAASSTSSLGKCCMWLWPWYRGGLTMKLHMSECVVTNDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CW51</accession>
    <name>A0A2K2CW51_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g09032v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CW51"/>
    <dbReference type="EnsemblPlants" id="PNT66252">
        <property type="protein sequence ID" value="PNT66252"/>
        <property type="gene ID" value="BRADI_3g09032v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66252">
        <property type="protein sequence ID" value="PNT66252"/>
        <property type="gene ID" value="BRADI_3g09032v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CW51"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="100" mass="10481" checksum="960611E27AA1599F" modified="2018-03-28" version="1">MVWGGLVLDSNALVLYGLNPWAEEQLASCPASSPSSPDPCWGPRVALPPSPPSGAPPPAYLGPPDSADPVPHAATRLFPKVLLCHKIPPRQLPFAPSDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2024-05-29" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D5E0</accession>
    <name>A0A2K2D5E0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g56543v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D5E0"/>
    <dbReference type="EnsemblPlants" id="PNT69495">
        <property type="protein sequence ID" value="PNT69495"/>
        <property type="gene ID" value="BRADI_3g56543v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69495">
        <property type="protein sequence ID" value="PNT69495"/>
        <property type="gene ID" value="BRADI_3g56543v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D5E0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT69495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="71" mass="8122" checksum="06B5B846721A5CC9" modified="2018-03-28" version="1">MVNEACKIEILHFFCTTIGCFKASTLVFMNLCWNFPVYNIFSLEFPIDTPSQGMGIGSRHKENHDSSNYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DAJ2</accession>
    <name>A0A2K2DAJ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100822942</name>
        <name evidence="12" type="ORF">BRADI_2g26000v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566262.2">
        <property type="nucleotide sequence ID" value="XM_003566214.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DAJ2"/>
    <dbReference type="STRING" id="15368.A0A2K2DAJ2"/>
    <dbReference type="EnsemblPlants" id="PNT71299">
        <property type="protein sequence ID" value="PNT71299"/>
        <property type="gene ID" value="BRADI_2g26000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822942"/>
    <dbReference type="Gramene" id="PNT71299">
        <property type="protein sequence ID" value="PNT71299"/>
        <property type="gene ID" value="BRADI_2g26000v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DAJ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06921">
        <property type="entry name" value="ChtBD1_GH19_hevein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF140">
        <property type="entry name" value="CHITINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 9" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="5 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chitin-binding type-1" evidence="11">
        <location>
            <begin position="42"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="11">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="77"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="124"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT71299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="413" mass="43230" checksum="C6500E5360717F14" modified="2018-03-28" version="1">MRHFSPYPSVVCKIKTMRAPSLATAVVLAILAAALATAQARAENCGSQAGGATCADNLCCSRFGFCGSSPEHCGDGCQSQCSGGGPGPGPTCGSQAGGATCANCLCCSRFGFCGSTPEHCGAGCQSQCSGCGPSPVPTPAPGPGPAPGPTPPGNEGVASILPRDLFERLLLHRNDNTCPARWFYTYDAFLAAAAAFPAFAGAEGNNNETRKREVAAFLGQTSHETTGGWDAAPDGPYSWGYCYKVEQQEPKPVYCEPSNEWPCTPGKKYYGRGPIQLSYNYNYGPAGQAPAIELDLLNNPDLVAADAVVAFKTALWFWMTPRDNKPSSHAVITGKWKPTAADIAAGRVPGYGVITNIINGGVECGIGPDLRVQDRIGFYRRYCDALGVDYGSNLDCDNQRPFSSGFPAGFAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DAX9</accession>
    <name>A0A2K2DAX9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g27657v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DAX9"/>
    <dbReference type="EnsemblPlants" id="PNT71443">
        <property type="protein sequence ID" value="PNT71443"/>
        <property type="gene ID" value="BRADI_2g27657v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71443">
        <property type="protein sequence ID" value="PNT71443"/>
        <property type="gene ID" value="BRADI_2g27657v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DAX9"/>
    <dbReference type="OrthoDB" id="1746383at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35218:SF9">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35218">
        <property type="entry name" value="RNASE H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT71443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10652" checksum="9BA965AEC79AB29B" modified="2018-03-28" version="1">MSLLSWNCRELGQPRTVHELLLLVRKHKPKILFLSETHNRKAMVEGLRWKLGLKHVITVTENSKGGGLALFWDEYALSCTESLVPTFDTTCGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2024-05-29" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DCG4</accession>
    <name>A0A2K2DCG4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g37802v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DCG4"/>
    <dbReference type="EnsemblPlants" id="PNT71959">
        <property type="protein sequence ID" value="PNT71959"/>
        <property type="gene ID" value="BRADI_2g37802v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71959">
        <property type="protein sequence ID" value="PNT71959"/>
        <property type="gene ID" value="BRADI_2g37802v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DCG4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT71959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="72" mass="8472" checksum="3DA0BAE3EECDB067" modified="2018-03-28" version="1">MCYTSLSDQPITNHNVQITFLPRFSYIHQTPNCNRTLSEILEKTKYFIGSNSYSKRLNEKTTNHLPHDLSWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DE33</accession>
    <name>A0A2K2DE33_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g45086v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DE33"/>
    <dbReference type="EnsemblPlants" id="PNT72489">
        <property type="protein sequence ID" value="PNT72489"/>
        <property type="gene ID" value="BRADI_2g45086v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72489">
        <property type="protein sequence ID" value="PNT72489"/>
        <property type="gene ID" value="BRADI_2g45086v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DE33"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT72489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="123" mass="12143" checksum="398AF0F55D6A40E6" modified="2018-03-28" version="1" fragment="single">RCVAEGEGGGGSHLGRRRGSGRPGRGPAEATAGSDEGREAPAGIGTARAAGRGRNRGGRRRTRGPCSSSSPRLATAALGSGRRSGRVGEAWGSRWGRPRWSPAAAPDAAGASRTGQGNAGGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DF41</accession>
    <name>A0A2K2DF41_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nitrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_2g49808v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Bcl-2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DF41"/>
    <dbReference type="FunCoup" id="A0A2K2DF41">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72859">
        <property type="protein sequence ID" value="PNT72859"/>
        <property type="gene ID" value="BRADI_2g49808v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72859">
        <property type="protein sequence ID" value="PNT72859"/>
        <property type="gene ID" value="BRADI_2g49808v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DF41"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DF41">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020726">
        <property type="entry name" value="Bcl2_BH2_motif_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01258">
        <property type="entry name" value="BH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT72859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="59403" checksum="ED6C808438EDF405" modified="2018-03-28" version="1">MLIMHVGLADASNLLTNYMGTSYMIAVLITVFADTVIGRYQTVIISSLIELVGLLLLTLQAHSSKLKPPECIFPSPTCQKVSGDNAVHLYVGLYLVAIGSAGIKAALPAHCADQFDEKDPKEKRQMSSFFNWLLLSICIGGAFSVTVFVWIQNKRGWDKGFGAATGVMGLALVAFVIGLPRYRISTVQGSSALLEIFRVYVAAIRNRNLRLPENPEELYEISRSKASPETEFVAHRDKPFRFLNKAAIVQTTTGEMPNPWRQCRVTQVENARTMLAMVPIFCCAIIMGTCLAQFQTFSIQQGATMDTRIAKHVQMPPATLPIIPLGMLICAVPIYERLFVPYARRITGHPNGILYLQRIGVGLVLSIVSMCIAAVVEMYRKRVATRNNMLDAIPQLQMLPISCFWLAPQYGVFGIADMFTFIGLLEFFYSQAPPALKSMSSAFLWCSMSLGYYFSSIIVRAVNVATKNSTASGGWLAGNNINRNHLDLFFWLLAVLSFLNFLNYLYWSRWYKYVKSQDQAEHVVVPMEQQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DHQ2</accession>
    <name>A0A2K2DHQ2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g01380v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT73784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DHQ2"/>
    <dbReference type="EnsemblPlants" id="PNT73784">
        <property type="protein sequence ID" value="PNT73784"/>
        <property type="gene ID" value="BRADI_1g01380v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73784">
        <property type="protein sequence ID" value="PNT73784"/>
        <property type="gene ID" value="BRADI_1g01380v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DHQ2"/>
    <dbReference type="OrthoDB" id="786072at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1167">
        <property type="entry name" value="OS08G0412100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="3">
        <location>
            <begin position="108"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="228"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT73784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="590" mass="66597" checksum="4CEDE11F385F6CEA" modified="2018-03-28" version="1">MEALSDHHSRRLFFNRIFGSGNCCSDMLEEVSNEILKKCGGLPLAIINISSLLANRRAVKEEWQKVKRSIGSALENNRSLEGMRSILSLSYNNLPLNLKTCLLYLSAFPEDYVIDRERLVRRWIAEGFISEERGQSQYEVAESYFYELINKSMVQPVDFEYDGKVRGCRVHDMMLEIIISKSAEDNFMTVLGSGQTSFANRHRFIRRLSIQHIDQELASALANEDLSHVRSVTVTSSGCMKHLPSLAEFEALRVLDFEGCEDLEYDMNGMDKLFQLKYLSLGRTHKSKLPQGIVMLGDLETLDLRGTGVQDLPSGIVRLIKLQHLLVQSGTKIPNGIGDMRNLRVLSGFTITQSRVDAVEDLGSLTSLHELNVNLDGGKHDEYKRHEEMLLSSSCKFGRCKLLTLRIYSHGSLEFLGSWSPPPSSLQLFHSFYYFPYVPRWITPALCSLSHINIFLLELTDEGLHTLGELPSLLYLEVWLKTGQKDRITVHGFPTLKVFNIFLNVALQVTFVKGAMPKLEDLTVPFHVSVAKSYGFYLGIEHLTCLKQARVWLYNNGATPSESKAAATAIRNEAGVHPNHPTVYIDGVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DIS3</accession>
    <name>A0A2K2DIS3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g09415v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DIS3"/>
    <dbReference type="EnsemblPlants" id="PNT74183">
        <property type="protein sequence ID" value="PNT74183"/>
        <property type="gene ID" value="BRADI_1g09415v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74183">
        <property type="protein sequence ID" value="PNT74183"/>
        <property type="gene ID" value="BRADI_1g09415v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DIS3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT74183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="70" mass="8078" checksum="EBB1C4A7AE0EDD07" modified="2018-03-28" version="1">MRAAPVLPRSARACVRSLFCFNEISVWILATKPNQMRFFTCLRKHRGALLHFLLGLVFQESGLREVISWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKI1</accession>
    <name>A0A2K2DKI1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g21835v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DKI1"/>
    <dbReference type="EnsemblPlants" id="PNT74781">
        <property type="protein sequence ID" value="PNT74781"/>
        <property type="gene ID" value="BRADI_1g21835v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74781">
        <property type="protein sequence ID" value="PNT74781"/>
        <property type="gene ID" value="BRADI_1g21835v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DKI1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT74781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="115" mass="12006" checksum="A4B132076378B8DD" modified="2018-03-28" version="1">MAASLGCILGATALRPTSLVGHGSEMVLVCLPAGRDAGLGFPESMRLCLASGIARPTMMKFSKQLGQGSDPSELDDFAAKMEEVKVLYARIINAAGLPEGEELAQAVTEPETGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKN4</accession>
    <name>A0A2K2DKN4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106865862</name>
        <name evidence="3" type="ORF">BRADI_1g22868v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752610.1">
        <property type="nucleotide sequence ID" value="XM_014897124.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DKN4"/>
    <dbReference type="EnsemblPlants" id="PNT74833">
        <property type="protein sequence ID" value="PNT74833"/>
        <property type="gene ID" value="BRADI_1g22868v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106865862"/>
    <dbReference type="Gramene" id="PNT74833">
        <property type="protein sequence ID" value="PNT74833"/>
        <property type="gene ID" value="BRADI_1g22868v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="679360at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
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        <location>
            <begin position="28"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT74833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12168" checksum="46D423F9DA38BD12" modified="2018-03-28" version="1">MAKIIKMAASILAVMLLLLTARAPSTARQYAAQGAQLLVFSTRYFILTAAVLTQTAFGFSLVRSAASAPTLASRRLHTVAAVACFVNLACKLYMVLLVSRGCGGESCSMHAPKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DLB1</accession>
    <name>A0A2K2DLB1_BRADI</name>
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            <fullName evidence="1">Rho-GAP domain-containing protein</fullName>
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        <name evidence="3" type="primary">LOC100846582</name>
        <name evidence="2" type="ORF">BRADI_1g27310v3</name>
    </gene>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DLB1"/>
    <dbReference type="STRING" id="15368.A0A2K2DLB1"/>
    <dbReference type="EnsemblPlants" id="PNT75065">
        <property type="protein sequence ID" value="PNT75065"/>
        <property type="gene ID" value="BRADI_1g27310v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75065">
        <property type="protein sequence ID" value="PNT75065"/>
        <property type="gene ID" value="BRADI_1g27310v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19923at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DLB1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47367">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47367:SF1">
        <property type="entry name" value="OS07G0486500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="133"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="371" mass="40798" checksum="F87C40E83EE26112" modified="2018-03-28" version="1">MPPVESPRWQQKATDFFSSSSFKLKQAGQSAGDNIADVAGKVGSVVKSRWAIFQDARQRPPPPGDTVQERFISAAANTGVILRKGISETKEKVAVGKVKVEEAAKKTADKSKTILNNIERWQKGVASTDVFGVPIEATVQREQSGKAVPLILAKCADYLVISGLSNEYLFRSEGDRKVLQQLVSLYNEDSGASLPDGVSPIDVAALIKCYLASIPEPLTTLALYGELRDARVSIDDLKNILKKLPNVNYMTLEFITALLLRVSRKSSLNKMDSRSLAVEFAPLIMWQQGDSGTDLRNHLRFTLKAPPKMVDTTSNTATWDLLDEDDVDASSQIPLDDMSPPDYSAIEVIQCLIEHHNPIFTDANETVWSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DMB3</accession>
    <name>A0A2K2DMB3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g31765v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DMB3"/>
    <dbReference type="EnsemblPlants" id="PNT75403">
        <property type="protein sequence ID" value="PNT75403"/>
        <property type="gene ID" value="BRADI_1g31765v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75403">
        <property type="protein sequence ID" value="PNT75403"/>
        <property type="gene ID" value="BRADI_1g31765v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DMB3"/>
    <dbReference type="OrthoDB" id="696293at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="19626" checksum="8E64298276A3407C" modified="2018-03-28" version="1">METRRRGQGAGGERAAAGMETRRRAVAPVSTEEAEGVELGNGGHGRSVGLLFTYALLLLFARAARSAAAATYRVASLAERRALSPGPGPAAAVPTHGTAAPSSKGGGMVEFMPKEEDLESDEAVWRLYERWCKDFNQKREPDEMARRFNKFKNRVLRVRDMNNSNADNPQVIQDGADQVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DPE8</accession>
    <name>A0A2K2DPE8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g45011v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DPE8"/>
    <dbReference type="EnsemblPlants" id="PNT76153">
        <property type="protein sequence ID" value="PNT76153"/>
        <property type="gene ID" value="BRADI_1g45011v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT76154">
        <property type="protein sequence ID" value="PNT76154"/>
        <property type="gene ID" value="BRADI_1g45011v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76153">
        <property type="protein sequence ID" value="PNT76153"/>
        <property type="gene ID" value="BRADI_1g45011v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76154">
        <property type="protein sequence ID" value="PNT76154"/>
        <property type="gene ID" value="BRADI_1g45011v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DPE8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="122" mass="12987" checksum="93AE27A11500558D" modified="2018-03-28" version="1" fragment="single">PSSAAPQPASPAKHLRSGHCARRTSASSPTRPPRDEAPPPPPLLCRASARLARKPPPVIRGHRATRHRRLLPSVALSAAPRPLASTCDLRTPSEPRDQASPPPPSPPHATRNSDRNPPPSHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQA6</accession>
    <name>A0A2K2DQA6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g48375v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQA6"/>
    <dbReference type="EnsemblPlants" id="PNT76462">
        <property type="protein sequence ID" value="PNT76462"/>
        <property type="gene ID" value="BRADI_1g48375v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76462">
        <property type="protein sequence ID" value="PNT76462"/>
        <property type="gene ID" value="BRADI_1g48375v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DQA6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="132" mass="14916" checksum="9415FC30CD34EF1C" modified="2018-03-28" version="1">METRGGIECGGLHKRWRPMVAEELHEGGELQEQRQWRRKRRPSKPVAARRRLRRCRAARSTLVGTPPSHLFPLPAFSPPPRPMSPTQFGRRPTGSPQIPTVSSASAHHLHCIHLGKLIWLVGLNFAVVMLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLP9</accession>
    <name>I1GLP9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methylenetetrahydrofolate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100846793</name>
        <name evidence="13" type="ORF">BRADI_1g04140v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19821"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563842.1">
        <property type="nucleotide sequence ID" value="XM_003563794.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLP9"/>
    <dbReference type="FunCoup" id="I1GLP9">
        <property type="interactions" value="1994"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GLP9"/>
    <dbReference type="EnsemblPlants" id="KQK12508">
        <property type="protein sequence ID" value="KQK12508"/>
        <property type="gene ID" value="BRADI_1g04140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846793"/>
    <dbReference type="Gramene" id="KQK12508">
        <property type="protein sequence ID" value="KQK12508"/>
        <property type="gene ID" value="BRADI_1g04140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846793"/>
    <dbReference type="eggNOG" id="KOG0564">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025841_2_2_1"/>
    <dbReference type="OMA" id="AWKEEFY"/>
    <dbReference type="OrthoDB" id="16284at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106312">
        <property type="term" value="F:methylenetetrahydrofolate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000005">
        <property type="entry name" value="Methylenetetrahydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21895">
        <property type="entry name" value="MTHFR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="12">
        <location>
            <begin position="310"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK12508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="594" mass="66267" checksum="8DA2107602E338FF" modified="2012-06-13" version="1">MKVIEKIQEAAANGRTVFSFEYFPPRTEEGVENLFERMDRMVAHGPNFCDITWGAGGSTADVTLDIANRMQNMVCVETMMHLTCTNMPVEKIDSALDTIKSNGIQNVLALRGDPPHGQDKFVQVAGGFSCALDLVQHIKAKYGDYFGITVAGYPEAHPEVILGEEGATEEAYSNDLAYLKRKVDAGADLIVTQLFYDTDMFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCKTKIPAEITAALDPIKDNEEAVKAYGIHLGTEMCKKILASGINTLHLYTLNMEKTALAILMNLGLIEESKLSRTLPWRPPTNVFRVKEDVRPIFWANRPKSYISRTLGWDQYPHGRWGDSRNPSYGALTDYQFTRPRGSGKKLQEEWAVPLKSVQDINERFMNLCQGKLTSSPWSELDGLQPETKIIDDQLVKINSKGFLTINSQPAVNAEKSDSPSVGWGGPGGYVYQKAYAEFFCAKDKLDQLVEKSKAFPSLTYIAVNKEGETFSNVPANAVNAVTWGVFPGKEVIQPTVVDSASFMVWKDEAFEIWSKGWACLFPEGDSSKELLEQVQKSYYLVSLVDNDYIHGDLFAAFKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GN28</accession>
    <name>I1GN28_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein ENHANCED DISEASE RESISTANCE 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100831685</name>
        <name evidence="3" type="ORF">BRADI_1g07960v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="BRADI_1g07960v3"/>
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        <property type="gene ID" value="BRADI_1g07960v3"/>
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        <property type="protein sequence ID" value="KQK13088"/>
        <property type="gene ID" value="BRADI_1g07960v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT74101"/>
        <property type="gene ID" value="BRADI_1g07960v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g07960v3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="BRADI_1g07960v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g07960v3"/>
    </dbReference>
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    <dbReference type="eggNOG" id="ENOG502QUGE">
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="entry name" value="EDR2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31558">
        <property type="entry name" value="CW14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS03G0759000 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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            <begin position="278"/>
            <end position="518"/>
        </location>
    </feature>
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        <location>
            <begin position="101"/>
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        </location>
    </feature>
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        <location>
            <begin position="162"/>
            <end position="210"/>
        </location>
    </feature>
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        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07059"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK13086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="534" mass="58874" checksum="C8A49DD11167EFF3" modified="2012-06-13" version="1">MGSCVSTTRRRRRSRRLSATARRFRRKVSAVIADAPVVFSGAGASNRFAARHEVLHVDAPPASGVTLHLTQLQWQHSQMDAGNVICEEAWYDSVSMLGESADYDSDFDDDDEDDDPDNDFASVSGDPLPDVVVPGGGGTNASPCKDAACLADTVQRLRSIANAEACQGDPPEKTDDDGSSAAADECLKEPQSAASCSPRPFPGSVPSNKVQPMPMPVAGVSPHHQRKKSAVVRLSFRRRSYEGDEMTEMSGSAKYLYRPRAGLTLPCSAGEKPSEGCWSVLEPSVFKVRGEGFFRDKKKSPAPNFSPYTPIGADMFASTRKVHHIAQHIALPSLKPHDAFPSLLIVNIQLPTYPTAMFGENDGDGINLVLYFKIADSFDKEISPQLKDSIKRLMNEEMEKVKGFPVDSNVPYTERLKILAGIVNPEDLQLSTTERKLVQTYNQKPVLSRPQHKFYKGSNYFEIDIDVHRFSFISRKGLETFRDRLKHGVIDLGLTIQAQKAEEVPEHVLCCMRLNKLDFADNGQIPTLITSSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQC5</accession>
    <name>I1GQC5_BRADI</name>
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100833355</name>
        <name evidence="3" type="ORF">BRADI_1g14830v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562293.1">
        <property type="nucleotide sequence ID" value="XM_003562245.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQC5"/>
    <dbReference type="STRING" id="15368.I1GQC5"/>
    <dbReference type="EnsemblPlants" id="KQK14222">
        <property type="protein sequence ID" value="KQK14222"/>
        <property type="gene ID" value="BRADI_1g14830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833355"/>
    <dbReference type="Gramene" id="KQK14222">
        <property type="protein sequence ID" value="KQK14222"/>
        <property type="gene ID" value="BRADI_1g14830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833355"/>
    <dbReference type="eggNOG" id="ENOG502QR79">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053049_1_0_1"/>
    <dbReference type="OMA" id="MHDSVNV"/>
    <dbReference type="OrthoDB" id="4115at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008479">
        <property type="entry name" value="DUF760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33598:SF2">
        <property type="entry name" value="MAR-BINDING FILAMENT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33598">
        <property type="entry name" value="OS02G0833400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05542">
        <property type="entry name" value="DUF760"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK14222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="393" mass="43758" checksum="8334B75674B87FD1" modified="2012-06-13" version="1">MPTASCLRPPLPKSAARRLRFPPPPPPAQLYPAGLAIGSRRRRLAGVGVAAASASPFDELYARGRPIHGSSKKSILWNLIQDIEPLDLSVIQKDVPPETVDAMKRTISGMLGLLPSDQFRVVVEALWNPFFKLLVSSIMTGYTLRNAEYRLSFERNLELPEEDSEYQKRDITEDNHHDINLGRPVTIFRLSEDDMLQDLGNFTPGGSMGEDLGDLTPQAEEHIIRLQSRLDAMKKELHDLKRKNSALQMQQFVGEEKNDLLDYLRSLTPEKVAELSESTCPGVQEAIQSVVHGLLATLSPKIYSKSPPPLGNAAGGVLNRGGEDDDCAELVENTSLPFQPLISVPRDYLARLLFWCMLLGHYIRGLEYRLELAQLLRMSSDVGSFTIGDDYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRD3</accession>
    <name>I1GRD3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 58</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100841812</name>
        <name evidence="10" type="ORF">BRADI_1g18440v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-18S rRNA processing. May bind microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562489.1">
        <property type="nucleotide sequence ID" value="XM_003562441.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRD3"/>
    <dbReference type="STRING" id="15368.I1GRD3"/>
    <dbReference type="EnsemblPlants" id="KQK14761">
        <property type="protein sequence ID" value="KQK14761"/>
        <property type="gene ID" value="BRADI_1g18440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841812"/>
    <dbReference type="Gramene" id="KQK14761">
        <property type="protein sequence ID" value="KQK14761"/>
        <property type="gene ID" value="BRADI_1g18440v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841812"/>
    <dbReference type="eggNOG" id="KOG2572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="ASWMQKF"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GRD3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000003">
        <property type="entry name" value="Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000001">
        <property type="entry name" value="Probable Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF13">
        <property type="entry name" value="NOP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="9">
        <location>
            <begin position="280"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="438"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="445"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="549"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK14761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="560" mass="61832" checksum="2EBD181613AB05CE" modified="2012-06-13" version="1">MLVLFETPAGFALFKVLDEGKLSKVEDLWKDFASSDKARKVVELKAFNKFENTSDALSAATLLIDSKPSKGLRKFLQKHCDGETLAVADSKLGNAIKEKLKIDCRHDSAVMELMRGLRSQLTELMSGLAEHDLGPMSLGLSHSLSRYKLKFSPEKVDTMIIQAIGLLDDLDKELNTYAMRVREWYGWHFPELTKIVADNIHYAKAVKMMGNRVNAVNLDFSEILPEEVEAELKEAAVISMGTEVNDLDLLNIRELCDQVLALSEYRAQLYDYLKSRMNTIAPNLTALVGELVGARLIAHGGSLMNLAKQPGSTIQILGAEKALFRALKTKHATPKYGLIYHASLIGQAAPKHKGKISRSLAAKTALAIRYDALADGDDNSMGLESRIKLETRLRVLEGKELGRSAGSAKGKPKIEAYEKDRKGAGALITPAKTYNPASDLVLGKSTEETPKKSEVASKKRKHEEAEPTKEATEADGEQEKEKSKKKKKKSKDSEESPAADADGGEKKKKKKNKESEEAPLADADGEKKKKKKKSDAQDVPMETDESSKKEKKKKKKHADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSC8</accession>
    <name>I1GSC8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4057 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100826948</name>
        <name evidence="3" type="ORF">BRADI_1g21410v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562693.1">
        <property type="nucleotide sequence ID" value="XM_003562645.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSC8"/>
    <dbReference type="FunCoup" id="I1GSC8">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GSC8"/>
    <dbReference type="EnsemblPlants" id="KQK15239">
        <property type="protein sequence ID" value="KQK15239"/>
        <property type="gene ID" value="BRADI_1g21410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826948"/>
    <dbReference type="Gramene" id="KQK15239">
        <property type="protein sequence ID" value="KQK15239"/>
        <property type="gene ID" value="BRADI_1g21410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826948"/>
    <dbReference type="eggNOG" id="ENOG502QR9Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_904303_0_0_1"/>
    <dbReference type="OMA" id="GEPMKTA"/>
    <dbReference type="OrthoDB" id="1868458at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025131">
        <property type="entry name" value="DUF4057"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31132">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31132:SF12">
        <property type="entry name" value="OS07G0623600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13266">
        <property type="entry name" value="DUF4057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4057" evidence="2">
        <location>
            <begin position="3"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK15239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="294" mass="31257" checksum="8EF89D3EFD2AD74A" modified="2012-06-13" version="1">MERPAPVRKSHTNTADLLAWPEGPQQQDLLAAGATPPPNRRPHQPSEAIHKVVFGGQVTEEEAESLNKRKQCSAPKWKEMTGSGIFAAGSEVEEDESGNASATAIRPPSKNYQAISTISHISFAEEENVSPKKPTSIAEVAKQRELSGTLLSEDDSKLKKQISNAKSKELSGHDIFAPPEDPRPRNSENGSTSQTPGKNAQVSSFKFGEDDTDSVVKTAKKIPTKKFNDLTGNDIFKAGDGGAAAAAAAEKQLSEAKLKEMSGSNIFADGKAPSRDFLGGIRKPPGGESSIALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1GU66</accession>
    <name>I1GU66_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100834886</name>
        <name evidence="13" type="ORF">BRADI_1g26850v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the plant CAR protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563063.1">
        <property type="nucleotide sequence ID" value="XM_003563015.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GU66"/>
    <dbReference type="FunCoup" id="I1GU66">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16114">
        <property type="protein sequence ID" value="KQK16114"/>
        <property type="gene ID" value="BRADI_1g26850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834886"/>
    <dbReference type="Gramene" id="KQK16114">
        <property type="protein sequence ID" value="KQK16114"/>
        <property type="gene ID" value="BRADI_1g26850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834886"/>
    <dbReference type="eggNOG" id="KOG1030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106037_0_0_1"/>
    <dbReference type="OMA" id="FMLEVEQ"/>
    <dbReference type="OrthoDB" id="587122at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04038">
        <property type="entry name" value="C2_ArfGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044562">
        <property type="entry name" value="CAR1-11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933:SF4">
        <property type="entry name" value="OS07G0500300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933">
        <property type="entry name" value="PROTEIN C2-DOMAIN ABA-RELATED 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK16114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="168" mass="18431" checksum="65E07AC531FD4628" modified="2012-06-13" version="1">MASEQVIGKLSVRVVRGSNLIVADPLTHTSDPYVVLCYGSQKVKTSVQKKNANPLWNEVLQLPVTNPTKPVKLEVFDEDKFTADDSMGVAEFNVTDIYDAAKLDLKHASDGTRIKTIYPVGTNYLGGESHVSWKNGKVVQDLILKLKNVDSGSIVLQVEWVHVPGVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1GVM6</accession>
    <name>I1GVM6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100840799</name>
        <name evidence="3" type="ORF">BRADI_1g31090v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240030.1">
        <property type="nucleotide sequence ID" value="XM_010241728.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GVM6"/>
    <dbReference type="STRING" id="15368.I1GVM6"/>
    <dbReference type="EnsemblPlants" id="KQK16857">
        <property type="protein sequence ID" value="KQK16857"/>
        <property type="gene ID" value="BRADI_1g31090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840799"/>
    <dbReference type="Gramene" id="KQK16857">
        <property type="protein sequence ID" value="KQK16857"/>
        <property type="gene ID" value="BRADI_1g31090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840799"/>
    <dbReference type="eggNOG" id="ENOG502S02K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031929_0_1_1"/>
    <dbReference type="OMA" id="ETCHYEI"/>
    <dbReference type="OrthoDB" id="768076at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47487">
        <property type="entry name" value="OS06G0651300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47487:SF8">
        <property type="entry name" value="OS08G0270900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U1-type" evidence="2">
        <location>
            <begin position="199"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="U1-type" evidence="2">
        <location>
            <begin position="280"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK16857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="406" mass="45129" checksum="105C46B6F8C322F9" modified="2012-06-13" version="1">MEFRFRAGERGPTSPPPASSVSRPSDGYFVPRGMQGGPLPPPPPFEWDAAMRERIIQDEVKRRLVEEEVRREFEVHGGFGPGPFFAPDFVMPPPLMPMPMLPPPHTLPPMPFEGFGAWPGFDQFGPRGPVGFGQMMLPRGDERRWSPPRPKPKRTLELREIGPSERPEAVSSKMKVPKMKRKADEIAATTLPRKVQKPAKDWSCALCQMTATCEAGLNEHLEGRKHKAKLAKCGASNVINYVKNNLQTTTGNKDSTGPSDEPKKICILVDGAMHEVVQKNNYLWCDRCKVRCDSNVTMAGHLRGKKHSQLNKVWTSILAVRMNKKTEEGLASPCGSKLNANDCTEIPAVIMGDLDMAIKVDESGPIEIKKESTNMGTEVHENSPVETKKEDMDMTNDGIQLVPKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXE5</accession>
    <name>I1GXE5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100833555</name>
        <name evidence="9" type="ORF">BRADI_1g36270v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752367.1">
        <property type="nucleotide sequence ID" value="XM_014896881.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXE5"/>
    <dbReference type="STRING" id="15368.I1GXE5"/>
    <dbReference type="EnsemblPlants" id="KQK17720">
        <property type="protein sequence ID" value="KQK17720"/>
        <property type="gene ID" value="BRADI_1g36270v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833555"/>
    <dbReference type="Gramene" id="KQK17720">
        <property type="protein sequence ID" value="KQK17720"/>
        <property type="gene ID" value="BRADI_1g36270v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833555"/>
    <dbReference type="eggNOG" id="KOG2887">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086833_0_0_1"/>
    <dbReference type="OMA" id="IALAYYC"/>
    <dbReference type="OrthoDB" id="660759at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK17720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="226" mass="24013" checksum="76619F840F44D5AD" modified="2012-06-13" version="1">MQAWFSGSGPSPSSSAAASSQAPSLLAEWNSYASARSAEEEAGGGFGIDIEAAVRSANDRVAGTFGVVSKGVKGLPSFQSTTSSVPSGKSLMYFGLFLASGIFLVFIAFTIFLPVMVIMPQKFAICFTIGCAFIIGSFFALKGPKNQLYHMISKERLPFTIGFLGSMFATIYVSMVLHSYILSVFFSCLQILALAYYAISYFPGGSAGMKFLSSALVASVLRCFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYX2</accession>
    <name>I1GYX2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">B-keto acyl reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100821460</name>
        <name evidence="6" type="ORF">BRADI_1g43170v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYX2"/>
    <dbReference type="STRING" id="15368.I1GYX2"/>
    <dbReference type="EnsemblPlants" id="KQK18539">
        <property type="protein sequence ID" value="KQK18539"/>
        <property type="gene ID" value="BRADI_1g43170v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18539">
        <property type="protein sequence ID" value="KQK18539"/>
        <property type="gene ID" value="BRADI_1g43170v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRPU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_38_3_1"/>
    <dbReference type="OMA" id="FCASATW"/>
    <dbReference type="OrthoDB" id="680872at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045703">
        <property type="term" value="F:ketoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000438">
        <property type="entry name" value="Os06g0299100 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF38">
        <property type="entry name" value="OS06G0300200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="316"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK18539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="37857" checksum="190DE0277AE58CE6" modified="2012-06-13" version="1">MAAVPAWLLSSLASLGALYVSAVAIRFLSYLALILRRPKDLRCRYGSWAVITGPTAGIGRSMSLELARRGLNLVLVGRDPAKLRDISETISSTHAVQTRTVLFDFSLASTPQGEKAVRRLREAVEGLDVGVLVNNAGVAKPGAVYLHEVAVEAWVRMIRVNLWALTEVTAAVLPGMVERGKGAIVNIGSGSGSLLPSYPLYSVYSATKRYAAGFSRSLAVEYRRKGIDVQCQVPLLVETNMVSNDVKGSFVPQFVLAPDAYARDAVGWIGHGTLCVPSVAHRFQAWTISLSPDFGYDAHILNKHLQHRDIFRRLRPWRKNSQGNSRNGSVDVATQQDKSGVCSEAPTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZ71</accession>
    <name>I1GZ71_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-ketoacyl-CoA synthase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100839678</name>
        <name evidence="8" type="ORF">BRADI_1g44030v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563984.1">
        <property type="nucleotide sequence ID" value="XM_003563936.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZ71"/>
    <dbReference type="STRING" id="15368.I1GZ71"/>
    <dbReference type="EnsemblPlants" id="KQK18680">
        <property type="protein sequence ID" value="KQK18680"/>
        <property type="gene ID" value="BRADI_1g44030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839678"/>
    <dbReference type="Gramene" id="KQK18680">
        <property type="protein sequence ID" value="KQK18680"/>
        <property type="gene ID" value="BRADI_1g44030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839678"/>
    <dbReference type="eggNOG" id="ENOG502QPKZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013238_2_1_1"/>
    <dbReference type="OMA" id="HAKLVTY"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="FAE" evidence="6">
        <location>
            <begin position="72"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="7">
        <location>
            <begin position="382"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK18680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="491" mass="54392" checksum="1D1204D1722376A4" modified="2012-06-13" version="1">MSSSPQLMKRVKPVYQYVVNNFVVVLAVPLAVAGIVSAARVGPDELLERARALRPVHVFLAAFLPAAVAVLYLMMRPRSVYLVDYACFRTKPSCRVPFGTFLEHAKLVTYIEGASIDERSIRFMTRLLERSGLGEETCLPPAHHYIPPYRNMEASREEVELVIFSAIDDLLAKTGISPDAIDILVVNCSLFAPVPSFTDMIIRKYKMRSDVRNVHLSGMGCSAGLISVGLARNFLQVAPRGAHALVVSTETITPNYYVGKERAMLLPNCLFRMGGAAALLSTSRAKARFRLSRVVRTLTGAQDGAYRCVFQEEDAEGHRGINLSKDLMTIAGDALKANITAIGPLVLPASEQLLFALSFIARRVFSNTRVKPYLPDFRMAFEHFCIHAGGRAVIDELQSSLGLSDEHVEASRMALHRFGNTSSSSLWYELAYIEAKGRMRKGDRVWMIGFGSGFKCNSAAWECIQPARDAHGPWAESICRYPVYIPEVLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1H0U7</accession>
    <name>I1H0U7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100828871</name>
        <name evidence="7" type="ORF">BRADI_1g48740v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564292.1">
        <property type="nucleotide sequence ID" value="XM_003564244.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H0U7"/>
    <dbReference type="FunCoup" id="I1H0U7">
        <property type="interactions" value="2687"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H0U7"/>
    <dbReference type="EnsemblPlants" id="KQK19514">
        <property type="protein sequence ID" value="KQK19514"/>
        <property type="gene ID" value="BRADI_1g48740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828871"/>
    <dbReference type="Gramene" id="KQK19514">
        <property type="protein sequence ID" value="KQK19514"/>
        <property type="gene ID" value="BRADI_1g48740v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828871"/>
    <dbReference type="eggNOG" id="KOG3165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081098_0_1_1"/>
    <dbReference type="OMA" id="GMMDCLL"/>
    <dbReference type="OrthoDB" id="76105at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H0U7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09864">
        <property type="entry name" value="PIN_Fcf1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000004">
        <property type="entry name" value="rRNA-processing protein FCF1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037503">
        <property type="entry name" value="Fcf1_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="66"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK19514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="199" mass="22809" checksum="B557D1D89298CB45" modified="2012-06-13" version="1">MGRAKSKGPKFAAVKKMISKKTIKKYKDEVLDPRKKDTEKEKLGRNVPQVSSALFFSYNMALGPPYRVIVDTNFINFSIQNKLDLEKGMMDCLYAKCTPCITDCVMAELEKLGQKYRVALRIAKDPRFQRLACTHKGTYADDCIVERITQHKCYIVATCDRDLKRRIRKVPGVPIMYITQHRYSIERLPEATIGGAPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1H119</accession>
    <name>I1H119_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100841599</name>
        <name evidence="3" type="ORF">BRADI_1g49330v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561101.1">
        <property type="nucleotide sequence ID" value="XM_003561053.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H119"/>
    <dbReference type="EnsemblPlants" id="KQK19616">
        <property type="protein sequence ID" value="KQK19616"/>
        <property type="gene ID" value="BRADI_1g49330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841599"/>
    <dbReference type="Gramene" id="KQK19616">
        <property type="protein sequence ID" value="KQK19616"/>
        <property type="gene ID" value="BRADI_1g49330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841599"/>
    <dbReference type="eggNOG" id="ENOG502R6SU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014546_2_0_1"/>
    <dbReference type="OrthoDB" id="444127at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF56">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK19616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="431" mass="45352" checksum="01B4F56BADF8A18C" modified="2012-06-13" version="1">MAKSAAALITPARATPGGVLPLSSIDRSIVGGLQAHTVQVFDRPPSSACAVAALREGLARALVPYYPVGGRVTPCGGAVDCTGELGVWLLEAAASGCDAAGVLDGRVPGELLLPGAEKLVSGAVLAAQVTRFACGGVAVGVSFSHAVMDGHGAGQFLCAVGEHARGVAPSVASPVWARDALIPDIPRSRPPRGTPPPPMPEPEPRRFVYQVADVSPDSIARVRKEYSQLTKKKACSVFDAVTAVVFKCRALALLATTTELGEELRLGFAVGTQHLVLPAGVAGYYGNCGNMARVRLSPAYSASASLGELVVAIREGKEKLEARVAGWLGGRDGYFDPAPFPVEDYGTVAVSDVRHLGFTEVDYGFGEPRYFFPPTHHLGFPNVYYVKPPKPKAGVRLLLRCVREPHAAAFAAQLAEFARGRDEVYTCRSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2M6</accession>
    <name>I1H2M6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100829026</name>
        <name evidence="2" type="ORF">BRADI_1g54030v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557382.1">
        <property type="nucleotide sequence ID" value="XM_003557334.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2M6"/>
    <dbReference type="FunCoup" id="I1H2M6">
        <property type="interactions" value="814"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H2M6"/>
    <dbReference type="EnsemblPlants" id="KQK20355">
        <property type="protein sequence ID" value="KQK20355"/>
        <property type="gene ID" value="BRADI_1g54030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829026"/>
    <dbReference type="Gramene" id="KQK20355">
        <property type="protein sequence ID" value="KQK20355"/>
        <property type="gene ID" value="BRADI_1g54030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829026"/>
    <dbReference type="eggNOG" id="ENOG502QUUA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050934_0_1_1"/>
    <dbReference type="OMA" id="YKTTDEP"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005996">
        <property type="term" value="P:monosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000321">
        <property type="entry name" value="Chloroplast stem-loop binding protein of 41 kDa a, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725:SF6">
        <property type="entry name" value="CHLOROPLAST STEM-LOOP BINDING PROTEIN OF 41 KDA A, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="75"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK20355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="394" mass="41098" checksum="B30207FAB9EE312A" modified="2012-06-13" version="1">MAFFPAATATTAAAVAFTSTRCVSPRPSSPYLPRGVASSAIAARRRAAPRAAAVRFSVRAQAAKKSVLVVNTNSGGHAVIGFYFAKALLAAGHAVTVLTVGDEGSDKMKKPPFSRFSELTSAGAKTVWGDPADVGAAVGGASFDVVLDNNGKDLDAVKPVADWAKSAGVGQFLFISSAGIYTPTDEPPHVEGDAVKGSAGHVGVEKYIAAEFGSWASFRPQYMIGSGNNKDCEEWFFDRIVRKRPVPIPGSGMQLTNISHARDLGSMLTMAVESPDAAAGKIFNCVSDRAVTLDGLAKMCAAAAGAAVEIVHYDPAAAGVDAKKAFPFRNMHFYAEPRAAKEVLGWTSTTNLPEDLKERFAEYASSGRGDKAMSFDLDDKILSAVGAAPVSVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2Q5</accession>
    <name>I1H2Q5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Methyltransferase</fullName>
            <ecNumber evidence="14">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100834229</name>
        <name evidence="16" type="ORF">BRADI_1g54305v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Steroid biosynthesis; sterol biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 13 14">Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="14"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228238.1">
        <property type="nucleotide sequence ID" value="XM_010229936.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752873.1">
        <property type="nucleotide sequence ID" value="XM_014897387.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2Q5"/>
    <dbReference type="STRING" id="15368.I1H2Q5"/>
    <dbReference type="EnsemblPlants" id="KQK20403">
        <property type="protein sequence ID" value="KQK20403"/>
        <property type="gene ID" value="BRADI_1g54305v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834229"/>
    <dbReference type="Gramene" id="KQK20403">
        <property type="protein sequence ID" value="KQK20403"/>
        <property type="gene ID" value="BRADI_1g54305v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834229"/>
    <dbReference type="eggNOG" id="KOG1269">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039068_5_0_1"/>
    <dbReference type="OMA" id="NGIATMM"/>
    <dbReference type="OrthoDB" id="4310724at2759"/>
    <dbReference type="UniPathway" id="UPA00766"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003838">
        <property type="term" value="F:sterol 24-C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000161">
        <property type="entry name" value="Methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050447">
        <property type="entry name" value="Erg6_SMT_methyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030384">
        <property type="entry name" value="MeTrfase_SMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013705">
        <property type="entry name" value="Sterol_MeTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068:SF1">
        <property type="entry name" value="HYPOTHETICAL LOC100005854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068">
        <property type="entry name" value="ZGC:194242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08498">
        <property type="entry name" value="Sterol_MT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51685">
        <property type="entry name" value="SAM_MT_ERG6_SMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="7" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="9" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase Erg6/SMT-type" evidence="15">
        <location>
            <begin position="54"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK20403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="344" mass="38853" checksum="A9F965054B9ABD9A" modified="2012-06-13" version="1">MSKTGALDLASGLGGKITKEEVKSAVDEYEKYHGYYGGKEEARKSNYTDMVNKYYDLATSFYEYGWGESFHFAHRWNGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQITRGKELNRLAGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRVLKPGQFFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIRSTRQCLQAVKDAGFEVIWDKDLAEDSPLPWYLPLDPSRFSLSSFRLTTVGRLITRNMVKALEYIGLAPQGSQRVSSFLEKAAEGLVEGGKKEIFTPMYFFVVRKPLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1H329</accession>
    <name>I1H329_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100831805</name>
        <name evidence="9" type="ORF">BRADI_1g55427v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561348.1">
        <property type="nucleotide sequence ID" value="XM_003561300.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H329"/>
    <dbReference type="STRING" id="15368.I1H329"/>
    <dbReference type="EnsemblPlants" id="KQK20575">
        <property type="protein sequence ID" value="KQK20575"/>
        <property type="gene ID" value="BRADI_1g55427v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831805"/>
    <dbReference type="Gramene" id="KQK20575">
        <property type="protein sequence ID" value="KQK20575"/>
        <property type="gene ID" value="BRADI_1g55427v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831805"/>
    <dbReference type="eggNOG" id="ENOG502QPM5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044273_1_0_1"/>
    <dbReference type="OMA" id="FRTHQPE"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H329">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080147">
        <property type="term" value="P:root hair cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000017">
        <property type="entry name" value="Transcription factor bHLH66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF198">
        <property type="entry name" value="DEHYDROGENASE, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="132"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="109"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK20575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="288" mass="30996" checksum="A03FF8CDE0ED245D" modified="2012-06-13" version="1">MAGQPPPGAEDDFLDHFFSIPSSFPAAGQGGAPAADGDHHPFPLALSLEAAAEASGGARRLQDGPDVARAERDPVQLAGLFAPVFAGAAPHLRPAPPSQVFHAQPRLGEGAMVPQPHQPPAPRPKQRARRGQATDPHSIAERLRRERIAERMRALQELVPNTNKTDRAAMLDEILDYVKFLRLQVKVLSMSRLGGAGAVAQLIADIPLSVKGEEASDSGGKQQIWEKWSTDGTEKQVAKLMEEDIGAAMQFLQSKALCMMPISLAMAIYDTQHLQDGHSMKPEPNSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1H403</accession>
    <name>I1H403_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100830763</name>
        <name evidence="17" type="ORF">BRADI_1g58260v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557639.1">
        <property type="nucleotide sequence ID" value="XM_003557591.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H403"/>
    <dbReference type="STRING" id="15368.I1H403"/>
    <dbReference type="EnsemblPlants" id="KQK21033">
        <property type="protein sequence ID" value="KQK21033"/>
        <property type="gene ID" value="BRADI_1g58260v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830763"/>
    <dbReference type="Gramene" id="KQK21033">
        <property type="protein sequence ID" value="KQK21033"/>
        <property type="gene ID" value="BRADI_1g58260v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830763"/>
    <dbReference type="eggNOG" id="ENOG502QUKN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_22_1_1"/>
    <dbReference type="OMA" id="IVCHYKQ"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009789">
        <property type="term" value="P:positive regulation of abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000486">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000686">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GHR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000480">
        <property type="entry name" value="Pollen receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053059">
        <property type="entry name" value="Inactive_SerThr-Kinase_ABA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48003:SF4">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GHR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48003">
        <property type="entry name" value="OS07G0626500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094280" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="23"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="643"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="786"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="614"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="674"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="619"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="677"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="735"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK21033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1068" mass="113955" checksum="2D3F14F39FDC0653" modified="2012-06-13" version="1">MGIVGGFFLVLLLLVVTPAASGQLPSQDILALLAFKKGITHDPAGFITDSWNDESIDFNGCPASWNGVVCNGASVAGVVLDGHRISGVADLSVFVNLTMLVKLSMANNNLSGSLPSKLGGLKSLKFLDISNNRFSGSIPDDIGSLRSLQNMSLARNNFSGPLPESIDGLTSLLSLDVSGNSLSGPLPAALKGLRSMVALNLSCNAFTKGIPAGLGLLVNLQSVDLSWNQLDGGVDWKFLIESSVAHVDFSRNLLTSTTPKELKFLADISETVLYLNLSSNKLTGSLIDGVELSTFGRLKVLDLSNNQLSGDLPGFNYVYDLEVLRLANNAFTGFVPSGLLKGDSLVLSELDLSANNLTGHINMITSTTLQILNLSSNALFGDLPLLAGSCTVLDLSNNQFRGNLSVLTKWSNDLEYVDLSQNNITGTIPDVSSQFLRLNYLNLSHNSLANTIPEAVVKYPKLTVLDLSSNQFSGLIPANLLTSSMLHELYIQDNMLTGGISFPGSSSKNLSLEVLDISGNHFSGSLPDDLASLSGLQVLDISSNNFSGPLPAAVSKIAALTALDISMNQFTGPLPEALPDTLQSLNASYNDLSGVVPVNLRKFPESSFHPGNSRLEYPASSSGSSGSASGSAGGKPLSTGAKIALVAASIVVLVIIILVAIVCHYKRISRQFPSSEKVSDKNLHRATKDIESMKRKDNKGGSEVSADDLAPRKGSTSEAPSQEEKLSAVGGFSPSKGSRFSWSPDSGEAYAQEGLARLDVRSPDRLAGELHFLDETITLTPEELSRAPAEVLGRSSHGTSYRATLENGVFLTVKWLREGVARPKKEFTKEAKKFSNIRHPNVVGLRGYYWGPTPHEKLILSDYVAPGSLASFLYDRPGRRGPPLTWAQRLKIAVDVARGLNYLHFDRAMPHGNLKASNILLDGLDLNARVADYCLHRLMTQAGVVEQILDLGVLGYRAPELAASKKPSPSFKSDVYAFGVALLELLTGRCAGDIVSGAEGGVDLTDWVRLRVAEGHGSECFDPAMASDSENPQAVKGMKEVLGIALRCIRPVSERPGIKSVYEDLSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1H8G7</accession>
    <name>I1H8G7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GH10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100835383</name>
        <name evidence="8" type="ORF">BRADI_1g71040v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
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        <text evidence="1">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
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        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
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        <property type="term" value="P:xylan catabolic process"/>
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        <property type="entry name" value="Galactose-binding domain-like"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 10 PROTEIN"/>
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    </dbReference>
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        <location>
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            <end position="30"/>
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        <location>
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            <end position="520"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007495"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK23063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1HBI1</accession>
    <name>I1HBI1_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BRADI_2g01600v3</name>
    </gene>
    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.I1HBI1"/>
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        <property type="gene ID" value="BRADI_2g01600v3"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OS01G0121600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK02466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="143" mass="14963" checksum="A01DF42C68483074" modified="2012-06-13" version="1">MSFSPSLSFPATLLPVRRPAAPAAARSRTRCGAAPGPRDSRYHGGALVDSGMAVLRRRIREARMAETNYEAPPGWAGWEKRYYPAYVSDVSALAGALQLLAMGTRPTVAAAVVAVLLAGVPVSAAVLLHHLAVAAEAALHHVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HDG4</accession>
    <name>I1HDG4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Membrane-associated kinase regulator 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100832942</name>
        <name evidence="2" type="ORF">BRADI_2g07497v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003565466.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HDG4"/>
    <dbReference type="FunCoup" id="I1HDG4">
        <property type="interactions" value="1753"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HDG4"/>
    <dbReference type="EnsemblPlants" id="KQK03377">
        <property type="protein sequence ID" value="KQK03377"/>
        <property type="gene ID" value="BRADI_2g07497v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832942"/>
    <dbReference type="Gramene" id="KQK03377">
        <property type="protein sequence ID" value="KQK03377"/>
        <property type="gene ID" value="BRADI_2g07497v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832942"/>
    <dbReference type="eggNOG" id="ENOG502QRKI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056837_0_0_1"/>
    <dbReference type="OMA" id="PVPAECN"/>
    <dbReference type="OrthoDB" id="689803at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039619">
        <property type="entry name" value="MAKR2/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33929">
        <property type="entry name" value="MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33929:SF1">
        <property type="entry name" value="MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="43864" checksum="34E446D34C962CB2" modified="2012-06-13" version="1">MESFSFLKFLRGAGVAGTERTAVAATTIAASAAACEDGGVDDDAAFFDLEFAVPGDESAASDAEEERVEFNFSVAGDAASGGEVVAVDPVVAPGDDAKEEEEAEAAAETAQQQQPPAASFLRPATTKFRVLLLKLRKPKAPPAPSADGNGCPAPKTAANRFLIKFRPVDDAPFVSLFTRDNSSRTSDAGSARPASQAPTMATSDASAAAAITAEERRFAKEVMLKYLNKIRPLYDKVSRRYGERLRFAGEGEETDAEPEPSPSPSPSPSPSPAAAPRAAEPQQAQQPVVVVACGVRAPRASVPAGLKQACKRLGKSRSASSAVAAAPSPSPTTTPSQQPQRRDDSLLQLQDGIQSAIAHCKRSFNASKGSESPLLRSMTDPSPKVDLTAADNKDGSDGASIKRAVRPAEKLMQEEQAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1HFU1</accession>
    <name>I1HFU1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BRADI_2g14510v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HFU1"/>
    <dbReference type="FunCoup" id="I1HFU1">
        <property type="interactions" value="312"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HFU1"/>
    <dbReference type="EnsemblPlants" id="KQK04596">
        <property type="protein sequence ID" value="KQK04596"/>
        <property type="gene ID" value="BRADI_2g14510v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04596">
        <property type="protein sequence ID" value="KQK04596"/>
        <property type="gene ID" value="BRADI_2g14510v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_4_1_1"/>
    <dbReference type="InParanoid" id="I1HFU1"/>
    <dbReference type="OMA" id="SIWVTFR"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000083">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000035">
        <property type="entry name" value="Putative receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052232">
        <property type="entry name" value="RLK_Ser/Thr-Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984">
        <property type="entry name" value="OS01G0323000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984:SF38">
        <property type="entry name" value="OS10G0497600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="189"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="448"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="458"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="480"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK04596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="509" mass="56878" checksum="6927399F7812C5DD" modified="2012-06-13" version="1">MPTAESLRAELSSKTPPFGLRLWIVIGISIWVVILCILGFMCFWSIYRRKPKKSVDKIPVSQIPDVSKEIAVDEVRQHAVVENYQVQESHALTVQEKSQDKDSGKMLGHLVRTKSSDADNLSQCSSVYQCDRAGSSYSGDEGSSGNARRQYSQYATVSASPLVGLPEFSHLGWGHWFTLRDLEHSTNRFSKENIIGEGGYGVVYRGRLINGTDVAIKKLLNNMGQAEKEFRVEVEAIGHVRHKNLVRLLGYCVEGIHRMLVYEYVNNGNLEQWIHGAMRQHGVLTWEARMKIILGIAKALAYLHEAIEPKVVHRDIKSSNILIDEDFNGKLSDFGLAKLLGAGKSHVTTRVMGTFGYVAPEYANTGLLNEKSDVYSFGVLLLEAVTGRDPVDNSRPDTEVHLVEWLKSMVGSRRAEEVVDPDMEVKPTIRALKRALLVALRCVDPHSEKRPTMGHVVRMLEAEDVPSREDRRSRRGHGGNTDTESKASSSEFETSGDRRDSGTSVRFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1HNR1</accession>
    <name>I1HNR1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100839699</name>
        <name evidence="13" type="ORF">BRADI_2g41500v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for the accumulation of coenzyme A in the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569230.1">
        <property type="nucleotide sequence ID" value="XM_003569182.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HNR1"/>
    <dbReference type="FunCoup" id="I1HNR1">
        <property type="interactions" value="1184"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HNR1"/>
    <dbReference type="EnsemblPlants" id="KQK08371">
        <property type="protein sequence ID" value="KQK08371"/>
        <property type="gene ID" value="BRADI_2g41500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839699"/>
    <dbReference type="Gramene" id="KQK08371">
        <property type="protein sequence ID" value="KQK08371"/>
        <property type="gene ID" value="BRADI_2g41500v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839699"/>
    <dbReference type="eggNOG" id="KOG0752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_10_1_1"/>
    <dbReference type="OMA" id="KMAPMVA"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HNR1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990559">
        <property type="term" value="P:mitochondrial coenzyme A transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000014">
        <property type="entry name" value="mitochondrial coenzyme A transporter SLC25A42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="25"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="121"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="228"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK08371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="36681" checksum="BEC062397450CEA4" modified="2012-06-13" version="1">MGSSQESTFSSASTAAQVNALDLLPVYAKELIAGGAAGAFAKTAVAPLERVKILLQTRTEGFQSLGIVRSLRKLWQYEGIRGFYKGNGASVLRIVPYAALHYMTYEQYRCWILNNAPSVGTGPVVDLLAGSAAGGTAVLCTYPLDLARTKLAYQVSNVAQPGNSLGNSGHQPVYNGVKDVFKTVYKEGGARSLYRGIGPTLIGILPYAGLKFYIYEDLKSRVPEDYKRSVVLKLSCGALAGLFGQTLTYPLDVVRRQMQVQNKQPQNVNDTFRIRGTFQGLLLIIRCQGWRQLFAGLSLNYVKVVPSVAIGFTTYDMMKNLLGVPPRERAHPSAGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSY8</accession>
    <name>I1HSY8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100840415</name>
        <name evidence="8" type="ORF">BRADI_2g53730v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232499.1">
        <property type="nucleotide sequence ID" value="XM_010234197.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSY8"/>
    <dbReference type="FunCoup" id="I1HSY8">
        <property type="interactions" value="742"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HSY8"/>
    <dbReference type="EnsemblPlants" id="KQK10381">
        <property type="protein sequence ID" value="KQK10381"/>
        <property type="gene ID" value="BRADI_2g53730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840415"/>
    <dbReference type="Gramene" id="KQK10381">
        <property type="protein sequence ID" value="KQK10381"/>
        <property type="gene ID" value="BRADI_2g53730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840415"/>
    <dbReference type="eggNOG" id="KOG1305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="28208at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HSY8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF323">
        <property type="entry name" value="AMINO ACID TRANSPORTER AVT6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="43"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK10381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="463" mass="48070" checksum="5CCC6E0DDDC82917" modified="2012-06-13" version="1">MASKNSRGLVTAGVGGEAAEAPLLLPEHAVVGDAEPPPAGAGGASVLGAVFNVSTSVVGAGIMSIPASMRVLGVAPAVALIVGVALLANAAVEFMLRYTRAGSLATTSYAALMGGSFGRAGAALLNVFVAFNCVGTLTVYLIIIGDVMSGPASGGEVHAGVLTEWFGPHLWTGREAVLVAAAVILLPLVLRKRVDSLRFTSAVSILLAAVFMLISMGIALYALFSGTAKVPRMLPDFSRLSSPFELFTAVPVIVVAFTFHFNVHPIRAELSKTSDMKAATRISLVLCSVIYAAVGFFGFLLFGDATMPDVLANFDSSSGSGVPQALNDAARLSYALHLVLVFPLLHYSLRVNVDELLFPGRRPLAADTRRFVSLTAALMAALYALAIAIPSIWTLFEYTGSTFAVCISLIFPGAIVLRDAHGIAKRKDKTLAATMITLAVITSSVAIASNIMSSVRGAQAKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HV89</accession>
    <name>I1HV89_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100845484</name>
        <name evidence="4" type="ORF">BRADI_2g60892v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232822.1">
        <property type="nucleotide sequence ID" value="XM_010234520.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HV89"/>
    <dbReference type="STRING" id="15368.I1HV89"/>
    <dbReference type="EnsemblPlants" id="PNT73607">
        <property type="protein sequence ID" value="PNT73607"/>
        <property type="gene ID" value="BRADI_2g60892v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845484"/>
    <dbReference type="Gramene" id="PNT73607">
        <property type="protein sequence ID" value="PNT73607"/>
        <property type="gene ID" value="BRADI_2g60892v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845484"/>
    <dbReference type="eggNOG" id="ENOG502QTJX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014546_5_1_1"/>
    <dbReference type="OMA" id="SMHEHES"/>
    <dbReference type="OrthoDB" id="610638at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF16">
        <property type="entry name" value="OS08G0543400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT73607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="436" mass="47439" checksum="C97AC23C910D423E" modified="2012-06-13" version="1">MGAKEEVKVLESCLVMPGEETPTHGLWLSPLDLVLAGRGHTPAVYFYRFVSGSTGDFFDVAKLKAAMAKALVAFYPLAGRLRVDDSGRTEIDCAGQGVLFVVAHSDFAADDFNDCQPSPELRKLFVPHIDGSSGIVFGVQVTFLKCGGVALGTALHHVAADGVSAFHFFQTWSAFSRNNGENVTTTLDLPCHDRTLLRARCPPVVHPDSLDVFYPFKINPCEPLGPIINKIFAISDDQVDALKRACGGVSTFCAVTTHVWRCLCAARQLPSDTTTRLTFTANVRGRMRPPLPAHYFGNAIIWLTSAGKVHDVASPSKEMMASVASRIRGTIRRMDEEVVHSAIDYLEQQETGNKPAPPSGNSLSKTELRVVSWLGMPVYDADFGWGKPLMMLRAVVPRAGVVFLMDGGRGDGSVHILICMETAILTDFQRLLYAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZ30</accession>
    <name>I1HZ30_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NHL repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100824092</name>
        <name evidence="4" type="ORF">BRADI_3g08990v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571876.1">
        <property type="nucleotide sequence ID" value="XM_003571828.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZ30"/>
    <dbReference type="EnsemblPlants" id="KQJ94170">
        <property type="protein sequence ID" value="KQJ94170"/>
        <property type="gene ID" value="BRADI_3g08990v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824092"/>
    <dbReference type="Gramene" id="KQJ94170">
        <property type="protein sequence ID" value="KQJ94170"/>
        <property type="gene ID" value="BRADI_3g08990v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824092"/>
    <dbReference type="eggNOG" id="ENOG502QR9M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029743_0_0_1"/>
    <dbReference type="OMA" id="GATMDDK"/>
    <dbReference type="OrthoDB" id="342730at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13833">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13833:SF73">
        <property type="entry name" value="NHL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094923" description="NHL repeat-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="370"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ94170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="53594" checksum="FBB6909B647E4C6F" modified="2012-06-13" version="1">MRLRSLPPLSLLLLLFVLLQSEANAFASPTDNIVRQLSSVVKWPRGSSPHSPKQSSHSQYDGNVALQFESGYFVETLVEGDKLGVTPHTIRVSPLEGGELLAVDSAHSNIVRITPPLSEYSRARLVAGSFQGHAGHIDGKPIDARFKRPTGVAVDDTGNVYIADTANLAIRKIGDSGVTTIAGGKSNIPGYRDGPSEDAKFSTDFDVVYVKKMCSLLVIDRGNAALRKIALPQEDCTYQDAALLSSDIILVIGAVVAGYLFSVVQHGFGPSSSEKIEAPEDEKQESSTVGKPPLVVESLKEEPSAGWPSFGTLVADLLKLAIEGVGSLLFNIVPSRLQRVKRKTGLTPLKDRLVMPEDREETPIAQKLSSTPMRTETLHAPNTVNETAPKAQKSIKSSSKFRDSTLSSKHRSTKRPEYAEFYGSSETPQASAKVPKDRLRHRHREKSGEVAYGSGHPEPKPAEMKPADYNDPKYDHYNMRNKYGTDTGFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZQ6</accession>
    <name>I1HZQ6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100827393</name>
        <name evidence="3" type="ORF">BRADI_3g10840v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572827.1">
        <property type="nucleotide sequence ID" value="XM_003572779.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZQ6"/>
    <dbReference type="STRING" id="15368.I1HZQ6"/>
    <dbReference type="EnsemblPlants" id="KQJ94492">
        <property type="protein sequence ID" value="KQJ94492"/>
        <property type="gene ID" value="BRADI_3g10840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827393"/>
    <dbReference type="Gramene" id="KQJ94492">
        <property type="protein sequence ID" value="KQJ94492"/>
        <property type="gene ID" value="BRADI_3g10840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827393"/>
    <dbReference type="eggNOG" id="ENOG502QU95">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007765_1_0_1"/>
    <dbReference type="OMA" id="RFREWWF"/>
    <dbReference type="OrthoDB" id="2016709at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HZQ6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006927">
        <property type="entry name" value="DUF639"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31860:SF6">
        <property type="entry name" value="HEAT-INDUCIBLE TRANSCRIPTION REPRESSOR (DUF639)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31860">
        <property type="entry name" value="HEAT-INDUCIBLE TRANSCRIPTION REPRESSOR (DUF639)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04842">
        <property type="entry name" value="DUF639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="536"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="620"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ94492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="691" mass="76685" checksum="767F6A16343D0F7D" modified="2012-06-13" version="1">MAMMRTRDLLMEGFEGFVRDGSFKWPVPRRVPDDDDDPDRSPSAGKSASVAGLSPMASAVVTRCSRVLDVSMHDLQYDFDVQASDSIKQAKNFARNFLEYCCFRALGQVSQVAGYLADKSLRRLTFDMMLAWEVPSSSSQLTVKVEVDSTVSLEAFSRIAPAIPTVADVVTSSNLFDALSSSTGGRLSFPVYDKYLTGLDRAIKKMKTQSESSLLSGLRSQRGERVLDVDGTLTTQPVLEHVGISTWPGRLTLTDHAVYFEALKVVTYDKPKAYDLAEDLKQVVKPELTGPWGSRLFDKAVMYKSTTLTEPVIIEFPELAGHSRRDYWLAIISEVLYVHRFVRKFNINGIDKEEIILKASLGILRLQAIEELAFPVSSRYESLLMFNLCDKLPGGDVILETLASTISSRSSAQANQPGMSLGMRSMSAFAVLSNLGVVSPGNNERLLVGEVVVGEMSSLQKAVTESMNNYKKVELAQATVDGVKVDGLDTNLVVMKELLSPVSDLWRVLVSLALWDEPLKSLGFCLLSSYIIIRGWLVYFLVMVLLFSAIFMLLTRLTSQGKPMIEVKVTSPPPMNTMEQLLAVQNAISKVEQLVQDANIVLLKIRALLLAFPSQATDRAILALLAMALSLAFLPTRLLILAMFLEVFTNNSPVRRASTERCTRRLREWWFSIPAAPVVVEKDNKEDKKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1Q2</accession>
    <name>I1I1Q2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_3g17322v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1Q2"/>
    <dbReference type="EnsemblPlants" id="KQJ95455">
        <property type="protein sequence ID" value="KQJ95455"/>
        <property type="gene ID" value="BRADI_3g17322v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ95455"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ95455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="75" mass="8375" checksum="EC3A3B88FFEE3D5A" modified="2012-06-13" version="1">MLKLETELAFQDQLIEELETKVSEGQQQIEELKSELAEAVKMKAEIGRVRDVAVASWSVAITLGVVFICLVKMLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2Y6</accession>
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.I1I2Y6"/>
    <dbReference type="EnsemblPlants" id="KQJ96114">
        <property type="protein sequence ID" value="KQJ96114"/>
        <property type="gene ID" value="BRADI_3g21090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840134"/>
    <dbReference type="Gramene" id="KQJ96114">
        <property type="protein sequence ID" value="KQJ96114"/>
        <property type="gene ID" value="BRADI_3g21090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840134"/>
    <dbReference type="eggNOG" id="KOG1303">
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    </dbReference>
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    <dbReference type="OMA" id="WIGAYTI"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:amino acid transmembrane transport"/>
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    </dbReference>
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        <property type="entry name" value="AA_transpt_TM"/>
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        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
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        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ96114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="522" mass="57970" checksum="C96A71CC7B039B6D" modified="2012-06-13" version="1">MASETAAGSALADEKAEAMEQQEAGGKSRLSGLLWHGGSAYDAWFSCASNQVAQVLLTLPYSFAQLGMLSGILFQLFYGLLGSWTAYLISILYLEYRTRKEKDKVDFRNHVIQWFEVLDGLLGRHWRNVGLAFNCTFLLFGSVIQLIGCASNIYYVNDHLDKRTWTYIFGACCATTVFIPSFHNYRVWSFLGLLMTTYTAWYIAVASLVHGQVEGVRHSGPTTIMLYFTGATNILYTFGGHAVTVEIMHAMWRPQKFKAIYLLATLYVLTLTLPSASAAYWAFGDQLLTHSNALSLLPRDAWRDAAVVLMLIHQFITFGFACTPLYFVWEKLIGLHDCKSLCKRAAARLPVVVPIWFLAIIFPFFGPINSAVGSLLVSFTVYIIPAMAHMVTFRSPQSRENAVERPPRFAGGWTGAYVINSFVVAWVLVVGFGFGGWASITNFVQQVSTFGLFAKCYQCPPRPAASPFLSPPVAFSPSMPPTPFSFNFTGIFAPMSSTPSPAPAPMPFGLGHHHHRHHRHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1I442</accession>
    <name>I1I442_BRADI</name>
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        <recommendedName>
            <fullName evidence="4">DUF1677 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100841238</name>
        <name evidence="1" type="ORF">BRADI_3g27040v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96773"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11881" checksum="02F224A67A89C2F2" modified="2012-06-13" version="1">MEIESAKCECCGLREDCTAEYIAGVKADFGGRWLCGLCSESVRDEVAKKKKKKGGSSSSMDLEEAVRDHMSFCGKFSRRKPAFRVADGMRQMLRRRASDIVSTSSAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5R1</accession>
    <name>I1I5R1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pmr5/Cas1p GDSL/SGNH-like acyl-esterase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100825224</name>
        <name evidence="6" type="ORF">BRADI_3g32180v3</name>
    </gene>
    <organism evidence="7">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003574215.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5R1"/>
    <dbReference type="EnsemblPlants" id="KQJ97603">
        <property type="protein sequence ID" value="KQJ97603"/>
        <property type="gene ID" value="BRADI_3g32180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825224"/>
    <dbReference type="Gramene" id="KQJ97603">
        <property type="protein sequence ID" value="KQJ97603"/>
        <property type="gene ID" value="BRADI_3g32180v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825224"/>
    <dbReference type="eggNOG" id="ENOG502QSS7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_761527_0_0_1"/>
    <dbReference type="OMA" id="LWHMLHI"/>
    <dbReference type="OrthoDB" id="767975at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045492">
        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13533">
        <property type="entry name" value="N-ACETYLNEURAMINATE 9-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13533:SF31">
        <property type="entry name" value="PROTEIN ALTERED XYLOGLUCAN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ97603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="37580" checksum="83DCFBAF35BA8FFD" modified="2012-06-13" version="1">MVSAGAQVGVLAACVVLFVPMGLAGWHLSRNKVLFFSGALFVSLAVGVHLSPYLPSLPHLLVSSFFHPLHPAAPSSSSDSCIPFLHRVSWADADGGRAWSWPPSLAATCGFARLSRDDASLLLNGSWVMVAGDSQARLLVLALLRLLLEPAAAAAAEPELFRRHSDYHAAVPARGISVDFIWAPFESNLTRLLHEDLRLAPRTPDVLVLGSGLWHMLHFTDAQHYGDALASVADAANSLRTPLPVPPPHMFWLGLPRLVNHMLNTDAKRSHMNDTMLQAYNREVDERCILRRDGGPFRLLDVGKLTRGCEQQCTADGMHYGGVVYDAVMHIMLNALVIESQQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8L9</accession>
    <name>I1I8L9_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100830128</name>
        <name evidence="1" type="ORF">BRADI_3g40400v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574777.1">
        <property type="nucleotide sequence ID" value="XM_003574729.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8L9"/>
    <dbReference type="STRING" id="15368.I1I8L9"/>
    <dbReference type="EnsemblPlants" id="KQJ98994">
        <property type="protein sequence ID" value="KQJ98994"/>
        <property type="gene ID" value="BRADI_3g40400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830128"/>
    <dbReference type="Gramene" id="KQJ98994">
        <property type="protein sequence ID" value="KQJ98994"/>
        <property type="gene ID" value="BRADI_3g40400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830128"/>
    <dbReference type="eggNOG" id="KOG0017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127888_0_0_1"/>
    <dbReference type="OMA" id="WIGPRRC"/>
    <dbReference type="OrthoDB" id="2016860at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
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    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ98994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="133" mass="14520" checksum="A687FFBBA18C5683" modified="2012-06-13" version="1">MAAVQWCSCSAASLNRWCHRPSPSPPTMSTARHRHPRRIGCVSVPRELAAATAAEAPPEIEAAMEEEGVVECQECGGAGWLLCGFCKGKKNNVKSESSPRIYRRCPTCKAAGYILCARCRVYRCITFPETADT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8T9</accession>
    <name>I1I8T9_BRADI</name>
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        <recommendedName>
            <fullName evidence="2">RIN4 pathogenic type III effector avirulence factor Avr cleavage site domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100831447</name>
        <name evidence="3" type="ORF">BRADI_3g40950v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572426.1">
        <property type="nucleotide sequence ID" value="XM_003572378.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8T9"/>
    <dbReference type="STRING" id="15368.I1I8T9"/>
    <dbReference type="EnsemblPlants" id="KQJ99083">
        <property type="protein sequence ID" value="KQJ99083"/>
        <property type="gene ID" value="BRADI_3g40950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831447"/>
    <dbReference type="Gramene" id="KQJ99083">
        <property type="protein sequence ID" value="KQJ99083"/>
        <property type="gene ID" value="BRADI_3g40950v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831447"/>
    <dbReference type="eggNOG" id="ENOG502QQ5R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057946_1_1_1"/>
    <dbReference type="OMA" id="GRTSQNN"/>
    <dbReference type="OrthoDB" id="765662at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040387">
        <property type="entry name" value="RIN4/NOI4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008700">
        <property type="entry name" value="TypeIII_avirulence_cleave"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33159:SF6">
        <property type="entry name" value="RPM1-INTERACTING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33159">
        <property type="entry name" value="RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05627">
        <property type="entry name" value="AvrRpt-cleavage"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="RIN4 pathogenic type III effector avirulence factor Avr cleavage site" evidence="2">
        <location>
            <begin position="173"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ99083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="242" mass="25631" checksum="C4143811B083DED5" modified="2012-06-13" version="1">MAKQQQKNGAHVPKFGNWDNDGNVPYTLYFDNARKGKAPGAKPMNPNDPLENPDAFSSSYAAAEAAAPPPPQPPAPAAHHERRPSSEVPAPAPPLYPGSPFHRGEPPRRVSGGGGRTSGGGGGGAYSVEQSPSRSPLHPYSRAADYSEASGFGLVANSVDRPRPRRGNETPTRGSAVPKFGDWDSNPASADGYTHIFNKVREEKQTQAKTPGFGKDGAAARGPGQHDDGYVSSSRWCFGWCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8U3</accession>
    <name>I1I8U3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100839842</name>
        <name evidence="7" type="ORF">BRADI_3g40990v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003574761.4"/>
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    <dbReference type="AlphaFoldDB" id="I1I8U3"/>
    <dbReference type="EnsemblPlants" id="KQJ99087">
        <property type="protein sequence ID" value="KQJ99087"/>
        <property type="gene ID" value="BRADI_3g40990v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839842"/>
    <dbReference type="Gramene" id="KQJ99087">
        <property type="protein sequence ID" value="KQJ99087"/>
        <property type="gene ID" value="BRADI_3g40990v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839842"/>
    <dbReference type="eggNOG" id="ENOG502SF02">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46629">
        <property type="entry name" value="OS01G0917900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
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        <property type="entry name" value="ZF_RING_1"/>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="223"/>
            <end position="261"/>
        </location>
    </feature>
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            <begin position="101"/>
            <end position="213"/>
        </location>
    </feature>
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        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
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        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ99087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="271" mass="27590" checksum="F13C34959EDF8BF4" modified="2012-06-13" version="1">MEGGRVLRRSVTLADQLALTVGPSAAAVAGGGGGSCNLRDLLKLRDDDDAARAAAAAGRRAAAVTLASAMQADRLASSPSPSSSAAAAAAAARTLLDIIRDDQLPAPPGGGAGGDPSVRRAVSLPAPATSPPAAAAEVLQPPPPLTPTIPEAPTDRDQEEEGEERVSLMALLEQTERQWSSSAHDEPEPEPAEEPSQPPREEDDDAEPEPDASAAAGRGAGCCCVCMARAKGAAFIPCGHTFCRACARELLAGRGRCPLCNAAILDVLHIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1I935</accession>
    <name>I1I935_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">STI1/HOP DP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100827087</name>
        <name evidence="16" type="ORF">BRADI_3g41720v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Plastid</location>
            <location evidence="12">Chloroplast outer membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574852.1">
        <property type="nucleotide sequence ID" value="XM_003574804.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I935"/>
    <dbReference type="STRING" id="15368.I1I935"/>
    <dbReference type="EnsemblPlants" id="KQJ99200">
        <property type="protein sequence ID" value="KQJ99200"/>
        <property type="gene ID" value="BRADI_3g41720v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827087"/>
    <dbReference type="Gramene" id="KQJ99200">
        <property type="protein sequence ID" value="KQJ99200"/>
        <property type="gene ID" value="BRADI_3g41720v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827087"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057418_1_0_1"/>
    <dbReference type="OMA" id="AGYGMKE"/>
    <dbReference type="OrthoDB" id="341259at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I935">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000049">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000106">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041243">
        <property type="entry name" value="STI1/HOP_DP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24203:SF78">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24203">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17830">
        <property type="entry name" value="STI1-HOP_DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="STI1/HOP DP" evidence="15">
        <location>
            <begin position="140"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="245"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="278"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="187"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQJ99200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="337" mass="36356" checksum="D07A378914E862B7" modified="2012-06-13" version="1">MASGGDPSGFPDESKTKKPEGSSNGRQGVPPAPAAGFPGAFDFSSMQSLLNDPSIKEMADQIARDPAFNQMAEQLQKGAQSAGEQGTPPLDPHQYIETMQKVMENPQFMTMAERLGSALMQDPAMSSMLENLTSPTHKEELEERMSRIKEDPSLKPILDELENGGPAAMIKYWNDPETLQKIGQAMGTTMPFGPVSSAEPSGTEETEEDGDEDESIVHHTSSVGDDEGLKKALDGGADKDEEDSEGRRALHFACGYGEFKCAQILLEAGAAVDAVDKNKNTPLHYAAGYGRKECVDLLLKHGAAVTLQNMDGKTPIDVAKLNNQDEVLKLLEKDVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IAP8</accession>
    <name>I1IAP8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100826469</name>
        <name evidence="5" type="ORF">BRADI_3g46270v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235566.1">
        <property type="nucleotide sequence ID" value="XM_010237264.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235568.1">
        <property type="nucleotide sequence ID" value="XM_010237266.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014755384.1">
        <property type="nucleotide sequence ID" value="XM_014899898.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316349.1">
        <property type="nucleotide sequence ID" value="XM_024460581.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316350.1">
        <property type="nucleotide sequence ID" value="XM_024460582.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316351.1">
        <property type="nucleotide sequence ID" value="XM_024460583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IAP8"/>
    <dbReference type="EnsemblPlants" id="KQJ99957">
        <property type="protein sequence ID" value="KQJ99957"/>
        <property type="gene ID" value="BRADI_3g46270v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99958">
        <property type="protein sequence ID" value="KQJ99958"/>
        <property type="gene ID" value="BRADI_3g46270v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68855">
        <property type="protein sequence ID" value="PNT68855"/>
        <property type="gene ID" value="BRADI_3g46270v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826469"/>
    <dbReference type="Gramene" id="KQJ99957">
        <property type="protein sequence ID" value="KQJ99957"/>
        <property type="gene ID" value="BRADI_3g46270v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99958">
        <property type="protein sequence ID" value="KQJ99958"/>
        <property type="gene ID" value="BRADI_3g46270v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68855">
        <property type="protein sequence ID" value="PNT68855"/>
        <property type="gene ID" value="BRADI_3g46270v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826469"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_49_0_1"/>
    <dbReference type="OMA" id="VIFACNM"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IAP8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000294">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g09900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932">
        <property type="entry name" value="ATPASE EXPRESSION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932:SF45">
        <property type="entry name" value="OS02G0565400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="123"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="190"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="260"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="330"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="365"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="400"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="435"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="470"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="505"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ99957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="60590" checksum="E79A46B08B439CCD" modified="2012-06-13" version="1">MAAPPPAAAAPPPAMSALPTFASSHPYPSLPTTKPLTPNSRLNFAHAGVAASPNAVPHRAVSNDRLRGLVRRGDLEEALRLVESMSGLEPSAAPGPCAALIKKLCASGRTAEARRVLAACEPDVMAYNAMVAGYCVTGQLDAARRLVADMPMEPDSYTYNTLIRGLCGRGRTGNALVVLDDMLRRGCVPDVVTYTILLEATCKRSGYKQAMKLLDEMRAKGCAPDIITYNVVVNGICQEGRVDDAIEFLKSLPSHGCEPNTVSYNIVLKGLCTAERWEDAEKLMAEMSQKGYPPNVVTFNMLISFLCRRGLVEPAMEVLDQIPKYGCTPNSLSYNPILHAFCKQKKMDRAMAFVELMVSRGCYPDIVSYNTLLTALCRGGEVDAAVELLHQLKDKGCTPVLISYNTVIDGLTKAGKTKEALELLNEMVTKGLQPDIITYSTISSGLCREDRIEEAIRAFCKVQDMGIRPNTVLYNAILLGLCKRRETHNAIDLFIYMISNGCMPNESTYTILIEGLTYEGLVKEARELLGELCSRGVVSKGLINKAAIRLLDGTTHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IFK0</accession>
    <name>I1IFK0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100822440</name>
        <name evidence="3" type="ORF">BRADI_3g60090v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK02056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570700.1">
        <property type="nucleotide sequence ID" value="XM_003570652.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IFK0"/>
    <dbReference type="FunCoup" id="I1IFK0">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IFK0"/>
    <dbReference type="EnsemblPlants" id="KQK02056">
        <property type="protein sequence ID" value="KQK02056"/>
        <property type="gene ID" value="BRADI_3g60090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822440"/>
    <dbReference type="Gramene" id="KQK02056">
        <property type="protein sequence ID" value="KQK02056"/>
        <property type="gene ID" value="BRADI_3g60090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822440"/>
    <dbReference type="eggNOG" id="KOG0714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_11_0_1"/>
    <dbReference type="OMA" id="AQFHSFC"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IFK0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44743:SF5">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44743">
        <property type="entry name" value="PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="21"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK02056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="268" mass="28806" checksum="DA64C262AA9C240B" modified="2012-06-13" version="1">MATGGDKCGGKPAAAGVGGGDLYSVLGVNKECSDADLKVAYRKLAMRWHPDRCSSSSSTKHMEEAKEKFQEIQGAYSVLSDANKRFLYDVGVYEEHEEEDDDTLQGMGDFLGEMAHMMSQTQPARQESFEELQQLFVDMFQSDIESGFCNGPAKDHDPVQRQTRTFSTPPSPSPSPPPPLATVDEAASCNGINKRGSSAMGSGKPPRAGEVSGGHGQSEFCFGMSDAKQAPKARGGNASRRRNGQKQKLSSKHDVSSGDEMPRPHAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IGH1</accession>
    <name>I1IGH1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nuclear transcription factor Y subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100844028</name>
        <name evidence="10" type="ORF">BRADI_4g01820v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KQJ85808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KQJ85810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KQJ85811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_010238725.3"/>
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        <property type="nucleotide sequence ID" value="XM_010238726.3"/>
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        <property type="nucleotide sequence ID" value="XM_024463312.1"/>
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    <dbReference type="SMR" id="I1IGH1"/>
    <dbReference type="STRING" id="15368.I1IGH1"/>
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        <property type="protein sequence ID" value="KQJ85806"/>
        <property type="gene ID" value="BRADI_4g01820v3"/>
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    <dbReference type="EnsemblPlants" id="KQJ85808">
        <property type="protein sequence ID" value="KQJ85808"/>
        <property type="gene ID" value="BRADI_4g01820v3"/>
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        <property type="protein sequence ID" value="KQJ85810"/>
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        <property type="protein sequence ID" value="KQJ85811"/>
        <property type="gene ID" value="BRADI_4g01820v3"/>
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    <dbReference type="GeneID" id="100844028"/>
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        <property type="gene ID" value="BRADI_4g01820v3"/>
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        <property type="gene ID" value="BRADI_4g01820v3"/>
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        <property type="gene ID" value="BRADI_4g01820v3"/>
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        <property type="gene ID" value="BRADI_4g01820v3"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="entry name" value="NFYA"/>
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        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CCAATSUBUNTB"/>
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        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NFYA_HAP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="121"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ85806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="211" mass="22930" checksum="D0582D3E59FC1612" modified="2012-06-13" version="1">MTSVADGISGDHRADEQQQQQTQAGHEDQQEAPATSIGSQTMVVAPSTDYVMPYAHQEVCHAMGQIAYPSIDPYFYGAYGGQPMMHPPLVGMHPAGLPLPTDAIEEPVYVNAKQYNAILRRRQSRAKAESERKLIKGRKPYLHESRHQHALKRARGAGGRFLNAKSDDNEEHSDSSSKDKQNGVAPRSSGQPSTAPSSKGASPANQTGNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IH08</accession>
    <name>I1IH08_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyl-[acyl-carrier-protein] hydrolase</fullName>
            <ecNumber evidence="11">3.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100823326</name>
        <name evidence="14" type="ORF">BRADI_4g03210v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Plays an essential role in chain termination during de novo fatty acid synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Plastid</location>
            <location evidence="1 11">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the acyl-ACP thioesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.-" evidence="11"/>
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        <property type="protein sequence ID" value="KQJ86087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579227.1">
        <property type="nucleotide sequence ID" value="XM_003579179.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IH08"/>
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        <property type="protein sequence ID" value="KQJ86087"/>
        <property type="gene ID" value="BRADI_4g03210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823326"/>
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        <property type="protein sequence ID" value="KQJ86087"/>
        <property type="gene ID" value="BRADI_4g03210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823326"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="618395at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000014">
        <property type="entry name" value="Acyl-[acyl-carrier-protein] hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049427">
        <property type="entry name" value="Acyl-ACP_TE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002864">
        <property type="entry name" value="Acyl-ACP_thioesterase_NHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045023">
        <property type="entry name" value="FATA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31727:SF10">
        <property type="entry name" value="ACYL-[ACYL-CARRIER-PROTEIN] HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31727">
        <property type="entry name" value="OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01643">
        <property type="entry name" value="Acyl-ACP_TE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20791">
        <property type="entry name" value="Acyl-ACP_TE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10 11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 11" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-ACP thioesterase N-terminal hotdog" evidence="12">
        <location>
            <begin position="88"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-ACP thioesterase-like C-terminal" evidence="13">
        <location>
            <begin position="249"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ86087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="389" mass="43543" checksum="C43EAFD56206C129" modified="2012-06-13" version="1">MAHSTLSWSSSSSCILYCCSRVLINSEVRRGSKAAPAAVRVNGGVAALVSPMTVVDDDDGHKKMISKEKQEADPFRQAQIVAEAGARYYRQTVVVRSYEVGPDKTATLETLLNLLQETALNHVWMSGLLGDGFGATHGMIKNNLIWVVSRMHVQVDRYPIWGEVLEIETWVGSSGKNGMRRDWLIRGRSSGEVLVRATSTWVMMNKITRRLSKMPDEVRAEISPWFIHGRHAIIQDGHHDKIVKLDSNAEYVASGLKPKRSDLDMNQHVNNVKYVRWMLETIPDQFMQQNQLSSIILEYRKECGISDIVQSICQPDEDSIPTSPGDQNIVSMVNGHSLSPEIINGHHSLAGALLQQQWPTKYTHLLQLEAGADGHGEIVRGRTTWKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1ILI5</accession>
    <name>I1ILI5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g17670v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ILI5"/>
    <dbReference type="FunCoup" id="I1ILI5">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1ILI5"/>
    <dbReference type="EnsemblPlants" id="KQJ88420">
        <property type="protein sequence ID" value="KQJ88420"/>
        <property type="gene ID" value="BRADI_4g17670v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88420">
        <property type="protein sequence ID" value="KQJ88420"/>
        <property type="gene ID" value="BRADI_4g17670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:106866810"/>
    <dbReference type="eggNOG" id="ENOG502S7JS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102808_4_1_1"/>
    <dbReference type="OMA" id="QISFKYA"/>
    <dbReference type="OrthoDB" id="603213at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001709">
        <property type="term" value="P:cell fate determination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040361">
        <property type="entry name" value="TPD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33184">
        <property type="entry name" value="PROTEIN TAPETUM DETERMINANT 1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33184:SF50">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24068">
        <property type="entry name" value="TPD1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095450" evidence="2">
        <location>
            <begin position="25"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ88420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="126" mass="13733" checksum="A519C0DB1E90E5B8" modified="2012-06-13" version="1">MEAKLLKILAMLLAFCLCNTSCDALCSLSDLQVAQRTARTKVGRYQEFSVEVRNSCICSQTNVKLLCPGFQSAIRVDPAVIRADPDGQLCTLNDGRAVRSGDVIKFVYAWTTTFSFAPVNSTLACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1INZ4</accession>
    <name>I1INZ4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe-S metabolism associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100824233</name>
        <name evidence="7" type="ORF">BRADI_4g26940v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577911.1">
        <property type="nucleotide sequence ID" value="XM_003577863.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INZ4"/>
    <dbReference type="FunCoup" id="I1INZ4">
        <property type="interactions" value="875"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1INZ4"/>
    <dbReference type="EnsemblPlants" id="KQJ89640">
        <property type="protein sequence ID" value="KQJ89640"/>
        <property type="gene ID" value="BRADI_4g26940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824233"/>
    <dbReference type="Gramene" id="KQJ89640">
        <property type="protein sequence ID" value="KQJ89640"/>
        <property type="gene ID" value="BRADI_4g26940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824233"/>
    <dbReference type="eggNOG" id="KOG2313">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041968_0_0_1"/>
    <dbReference type="OMA" id="DSQFKTR"/>
    <dbReference type="OrthoDB" id="411584at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.90:FF:000004">
        <property type="entry name" value="SufE-like protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003808">
        <property type="entry name" value="Fe-S_metab-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230:SF3">
        <property type="entry name" value="SUFE-LIKE PROTEIN 1, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02657">
        <property type="entry name" value="SufE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Fe-S metabolism associated" evidence="6">
        <location>
            <begin position="76"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="236"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ89640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="361" mass="38614" checksum="CD991A4726D9F17C" modified="2012-06-13" version="1">MASAAATSTPLRLVSSARLSKPLLSKPHLLTVSSPVSFQRLTARSSASPTPSTSPSTSGSVDPAQLPPALRDIIALFQSVPDERTRYKQLLAYAARLPPMDPTLKTDANRVRGCVSQVWVHAAPEVDAPGCVRFQADSDAQLTKGLAALLVLGLSGAPARDVAMVPVEFIEMIGIRQSLSPSRNSGLLNMINLMKLKALEIAAGGDAAGKEGTGGQEIHQVVAQLRAVEKEEPQFSAFGAQEHETSQTERPEEEQFPDEPSAVVHGNGSLGMGRKERIRESLETGLSPVELEIEDISHLHKGHAGVAGSSGETHFNVRVVSKEFEGKSMLKRHRAVYDLLQDELKSGLHALSIHAKTPSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPH7</accession>
    <name>I1IPH7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAWADEE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100829241</name>
        <name evidence="2" type="ORF">BRADI_4g28440v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578009.1">
        <property type="nucleotide sequence ID" value="XM_003577961.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPH7"/>
    <dbReference type="STRING" id="15368.I1IPH7"/>
    <dbReference type="EnsemblPlants" id="KQJ89917">
        <property type="protein sequence ID" value="KQJ89917"/>
        <property type="gene ID" value="BRADI_4g28440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829241"/>
    <dbReference type="Gramene" id="KQJ89917">
        <property type="protein sequence ID" value="KQJ89917"/>
        <property type="gene ID" value="BRADI_4g28440v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829241"/>
    <dbReference type="HOGENOM" id="CLU_053618_0_0_1"/>
    <dbReference type="OrthoDB" id="1885884at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IPH7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032001">
        <property type="entry name" value="SAWADEE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039276">
        <property type="entry name" value="SHH1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33827:SF3">
        <property type="entry name" value="OS09G0346900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33827">
        <property type="entry name" value="PROTEIN SAWADEE HOMEODOMAIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16719">
        <property type="entry name" value="SAWADEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAWADEE" evidence="1">
        <location>
            <begin position="137"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ89917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="270" mass="30982" checksum="C97122D3F0391948" modified="2012-06-13" version="1">MERRSRIRFSPSEIARMEKLVSNRKERVFDENFCRKLAEEFNRSAARVGSRALQPTQVKGWFLDKFPASTTKPTCLLTISEEEKTLASEADAFVSEIKTPVSEGKVLGLDTSISNNEDALSMDLPKDTTDKVPELENLQFEARSSKDFAWYDIDNFMAHRTTSSGEVEVYVRFAGFGAEEDEWVNVRKSIRQQSIPLESSECRNIATGDLVLCFKESNDDALHFDGHVLDIQRKQHDIRGCRCVFLVEYDHDGSQERVNLKRLSRRPKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPX6</accession>
    <name>I1IPX6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100825868</name>
        <name evidence="2" type="ORF">BRADI_4g29670v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310983.1">
        <property type="nucleotide sequence ID" value="XM_024455215.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310984.1">
        <property type="nucleotide sequence ID" value="XM_024455216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPX6"/>
    <dbReference type="STRING" id="15368.I1IPX6"/>
    <dbReference type="MEROPS" id="S33.A11"/>
    <dbReference type="EnsemblPlants" id="KQJ90135">
        <property type="protein sequence ID" value="KQJ90135"/>
        <property type="gene ID" value="BRADI_4g29670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825868"/>
    <dbReference type="Gramene" id="KQJ90135">
        <property type="protein sequence ID" value="KQJ90135"/>
        <property type="gene ID" value="BRADI_4g29670v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026209_2_0_1"/>
    <dbReference type="OMA" id="YPEARHE"/>
    <dbReference type="OrthoDB" id="2498029at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000111">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="126"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ90135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="387" mass="42875" checksum="C29E79ECBCB291AB" modified="2012-06-13" version="1">MRAPAASPAGRALPSLRPAPAPRLSAVVRVVIPLPVATRERRLCVAVSRASEMAAAAEEGEEEEKRWWRGPVEMDAAVRRDMAIRRLQEEAEAAGMGRSRREFAVFETTRGDTLFTQSWTPAAADPIRGIVVLLHGLNEHSGRYDHFAKLLNDQGLKVYAMDWIGHGGSDGVHGYVSSLDHAVGDLKEFLEDVVLEENYGLPCFLFGHSTGGAIVLKAALDPCVKVHIEGLILTSPAIHVQPSHPIIKVVAPIFSVLAPKYRVSALHRRGHPVSRDPEALKIKYADPLVYTGPIRVRTGNEILRISSYLQRNLSRVTVPFLVLHGTADTITDPRASQRLYQASMSTNKSIKLYDGYLHDLLFEPERDEIANDIITWLSSRLNALQRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQV3</accession>
    <name>I1IQV3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100827424</name>
        <name evidence="12" type="ORF">BRADI_4g32510v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578254.1">
        <property type="nucleotide sequence ID" value="XM_003578206.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQV3"/>
    <dbReference type="EnsemblPlants" id="KQJ90569">
        <property type="protein sequence ID" value="KQJ90569"/>
        <property type="gene ID" value="BRADI_4g32510v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827424"/>
    <dbReference type="Gramene" id="KQJ90569">
        <property type="protein sequence ID" value="KQJ90569"/>
        <property type="gene ID" value="BRADI_4g32510v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827424"/>
    <dbReference type="eggNOG" id="KOG0759">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_14_1_1"/>
    <dbReference type="OMA" id="SMPFDIT"/>
    <dbReference type="OrthoDB" id="6703404at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015140">
        <property type="term" value="F:malate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015131">
        <property type="term" value="F:oxaloacetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015116">
        <property type="term" value="F:sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015117">
        <property type="term" value="F:thiosulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071423">
        <property type="term" value="P:malate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015729">
        <property type="term" value="P:oxaloacetate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071422">
        <property type="term" value="P:succinate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902358">
        <property type="term" value="P:sulfate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015709">
        <property type="term" value="P:thiosulfate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000030">
        <property type="entry name" value="Mitochondrial uncoupling protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00784">
        <property type="entry name" value="MTUNCOUPLING"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="4"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="122"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="223"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ90569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="319" mass="33051" checksum="09B0BB72C294BF41" modified="2012-06-13" version="1">MGLKGFVEGGAASVVAGCSTHPLDLIKVRMQLQGEAAPAAAPPMRLALAFPPGVSLQGQGQPPRKPGPVAVGAQILRAEGPAGFLSGVSATVLRQAVYSSTSMGLYDAIKKRWERDGGGGALPLHRKIAAGLVAGGVGATVGNPADVAMVRMQADGRLPAAERRNYRSVAHAIGRIARDEGVRSLWRGSSLTVNRAMIVTASQLATYDQAKEAILARRGPAGDGLATHVAASFTAGLVAAAASSPVDVVKTRVMNMKVQPGAPPPYAGALDCAIKTVRSEGALALYKGFIPTITRQGPFTVVLFVTLEQVRKVLKDFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I1IR61</accession>
    <name>I1IR61_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g33430v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ90712.1"/>
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        <property type="gene ID" value="BRADI_4g33430v3"/>
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        <property type="gene ID" value="BRADI_4g33430v3"/>
    </dbReference>
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            <begin position="131"/>
            <end position="141"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90712"/>
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    </evidence>
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</entry>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">Bd21</strain>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="protein sequence ID" value="KQJ83023"/>
        <property type="gene ID" value="BRADI_5g12680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828653"/>
    <dbReference type="Gramene" id="KQJ83023">
        <property type="protein sequence ID" value="KQJ83023"/>
        <property type="gene ID" value="BRADI_5g12680v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034146_0_0_1"/>
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        <property type="entry name" value="OS04G0465000 PROTEIN"/>
        <property type="match status" value="1"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ83023.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ83023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="398" mass="44173" checksum="4F4D45DC3A73602A" modified="2012-06-13" version="1">MASSAKVTITLGRSGQVVKRKAIADIGNDDEVPVSGRKRPVRERLGNNVVDSDSFGGQPRSKRRQTESNSSQLGDDSLVEVRQIGRDDLRLKLMKKGLSKKSNGDTEQNGVDLREKLSRNSKNLLRYEARGRDQESRPSYAVRDVLESRSRYGLRERVPESRASTLGSEMTSARSADDLFKMDSSGKPYPSWNADWGRSPDKLPSFRRDVSPPISVRRDMSPPRSVRRDMSPPRSVRRGMSPPRSVRRGMSPPRSVRRGMSPPGTYSQIRSIPPLRSVGTTRSSSHISRESPDTLRTHQHYEGKSTISNDTLQPANGITPSGPRLPTAPVMTEVPLTVTGLLNSLGLEKYVVLFQAEEVDMAALSQMKDSDLKDMGVPMGPRKKILLAAAPYAKQRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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            <strain evidence="9 10">Bd21</strain>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
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            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="protein sequence ID" value="KQJ83149.1"/>
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    <dbReference type="KEGG" id="bdi:100838152"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="8">
        <location>
            <begin position="259"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="531"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="8">
        <location>
            <begin position="858"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="147"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ83149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1028" mass="114052" checksum="00EA2692D0ADEAE2" modified="2012-06-13" version="1">MRLYVCVLEARGLPAPPHCAHGGCGCGAVYTRVKVGAQRARTRAVELAGPGSAAAWNEEFVFPVGVEEEGDEVVEVGVARRLEGAGREVLGRVKLPVPAVQAASGTQSMPPTWFTLQPKHRRKGGAADCGKILLTVSLYGENNDNALIHSSPCPSSRSDTDVEFERSTCREHSDLNGGAVDSPRNSAVERVSLDGSDRSIKAHSNSISEDDDTIESSPSTSKMALDSDTEPPIPDASFEEAMETMKSKGSTADIPEDLAGGKIFEHTYLVESKDLNSLLFGPDSQFSRDLRDLQGTMDYDEQPWTWKSQDPPSLTRTCRYTKGSTKLMKDVKTIEEQTYLKADGKNFVIMTRVRTPEVPFGNCFEVVLLYKITHCPELSSGEECSHLTVSYNVEFLQSTLMKSMIEGSVRDGVKENFESFTEILSRHVKLADSAGMDKEQLLAPLQTDHQSHIRLACKYFCNFTVMSTVIMAMYVLVHILLSKPGPLMGLEFTGLDLPDTFGELITSGILVLQMERLLNMISRFVEARIQRGGDHGVKATGDGWLLTVALLEATSLPPVSCGSVDPYVVFSCNGITRTSSVQLQTQEPQWNEIMEFDAMEEPPATLDVEVFNFDGPFDLAISLGHAEINFLKHTSAELADIWVPLVGKLAQTCQSRLHLRIFLENTKGPETSKREYLSKMEKEVGKKLHVRSPHRNSTFQKLFSLPHEEFLIADYACSLKRKLPLQGRLFLSARLVGFYANLFGHKTKFFFLWEDVEEVEVLPPSFTTVGTPSLVFTLKSGRGLDAKSGAKSQDKEGRLKFQFHSFASFSKANRTIIGLWKTKSSAIEQRAKLEEDREDESYDELDDVQTVLSTGDVSLSKEYTVEHPIDANLLMGVFDGGPLETRTMSKVGCLDYTATPWEQTKPGVLERHASYKFNRYMSIFGGEVVSTQLKSPSEDGAGWMVHDVMTLHNVPFGDYFRVHLRYDIRSVASEPASSRCEVYVGIEWLKSSKFQKRIARNICDKLANRAKEVLEVAGKEITTSALSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZH6</accession>
    <name>I1IZH6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100832099</name>
        <name evidence="7" type="ORF">BRADI_5g15220v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. CPYC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580104.1">
        <property type="nucleotide sequence ID" value="XM_003580056.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZH6"/>
    <dbReference type="STRING" id="15368.I1IZH6"/>
    <dbReference type="EnsemblPlants" id="KQJ83488">
        <property type="protein sequence ID" value="KQJ83488"/>
        <property type="gene ID" value="BRADI_5g15220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832099"/>
    <dbReference type="Gramene" id="KQJ83488">
        <property type="protein sequence ID" value="KQJ83488"/>
        <property type="gene ID" value="BRADI_5g15220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832099"/>
    <dbReference type="eggNOG" id="KOG1752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_7_2_1"/>
    <dbReference type="OMA" id="IYTSPLC"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IZH6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000093">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF11">
        <property type="entry name" value="GLUTAREDOXIN-C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="6">
        <location>
            <begin position="30"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ83488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="128" mass="13186" checksum="063071DD87F9E2AF" modified="2012-06-13" version="1">MGIASSSSSTPESRTMALAKAKEIVASAPVVVFSKSYCPFCVKVKQLFTQLGASFKAIELDKESDGAEMQSALAEWTGQRTVPNVFINGKHIGGCDDTVALNNGGKLVALLTEAGAIAGSASKATMTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1J125</accession>
    <name>I1J125_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FLZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100822608</name>
        <name evidence="6" type="ORF">BRADI_5g19650v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580412.1">
        <property type="nucleotide sequence ID" value="XM_003580364.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J125"/>
    <dbReference type="STRING" id="15368.I1J125"/>
    <dbReference type="EnsemblPlants" id="KQJ84260">
        <property type="protein sequence ID" value="KQJ84260"/>
        <property type="gene ID" value="BRADI_5g19650v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822608"/>
    <dbReference type="Gramene" id="KQJ84260">
        <property type="protein sequence ID" value="KQJ84260"/>
        <property type="gene ID" value="BRADI_5g19650v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822608"/>
    <dbReference type="eggNOG" id="ENOG502RZD3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121190_2_0_1"/>
    <dbReference type="OMA" id="QECRCEQ"/>
    <dbReference type="OrthoDB" id="1916924at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044533">
        <property type="entry name" value="FLZ1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007650">
        <property type="entry name" value="Zf-FLZ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46057">
        <property type="entry name" value="FCS-LIKE ZINC FINGER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46057:SF40">
        <property type="entry name" value="OS04G0586100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04570">
        <property type="entry name" value="zf-FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51795">
        <property type="entry name" value="ZF_FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FLZ-type" evidence="5">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="FLZ-type" evidence="3">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ84260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="100" mass="10792" checksum="CAAB77C73FCAACC7" modified="2012-06-13" version="1">MAASVACAFFFDAEPAGETGKHALDACALCTKPLARDSDIFMYRGDTPFCSQECRCEQMQLDAFYARQAAKAAARRQRQFSSGTEAGSGKPESREVPVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E1Z7</accession>
    <name>A0A0Q3E1Z7_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g02218v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E1Z7"/>
    <dbReference type="EnsemblPlants" id="KQJ81678">
        <property type="protein sequence ID" value="KQJ81678"/>
        <property type="gene ID" value="BRADI_5g02218v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81678">
        <property type="protein sequence ID" value="KQJ81678"/>
        <property type="gene ID" value="BRADI_5g02218v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E1Z7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ81678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="172" mass="17941" checksum="07BC53EF3D2B7DD9" modified="2016-01-20" version="1">MTWHPSFSAPPSQLLLSLPTSSLPLQPPLPSLCPDLSSLTPPPSLLPHPPRLSSPSSPSLLPQPATNGLSLPSPEPLLSFEHRPPTKAAGPRSGSPSPGSGGSGRLRPLPSPLSISPSLGSLLVLTLLFLSLPFLQTGRAPLWTARDAMEDPAPVVPCAASSGLTWGRRRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FAN7</accession>
    <name>A0A0Q3FAN7_BRADI</name>
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            <fullName evidence="5">Preprotein translocase subunit SECE1</fullName>
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        <name evidence="2" type="ORF">BRADI_3g27506v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ96862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ96863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_014899926.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FAN7"/>
    <dbReference type="STRING" id="15368.A0A0Q3FAN7"/>
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        <property type="protein sequence ID" value="KQJ96862"/>
        <property type="gene ID" value="BRADI_3g27506v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ96863">
        <property type="protein sequence ID" value="KQJ96863"/>
        <property type="gene ID" value="BRADI_3g27506v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g27506v3"/>
    </dbReference>
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            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ96863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="19814" checksum="57C17322F44D356D" modified="2016-01-20" version="1">MATTPFCRPVQSHCASLPLHRRLSCLFAPARSSPTARPSLSTLGSPALSFCRSISVRRRLAAASKDTASDKGQEQEPSMAAGEALKSGVDETASGEKTPEEVAAELKELMRARKEAEVAAGSTGAGAGWWDGVVQEMSEIEWPAPGKVLGTTGVVLGIIAGSTAALLSVNAILAELSDRVFAGRGLQDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FLY3</accession>
    <name>A0A0Q3FLY3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100829312</name>
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    </gene>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Stipodae</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK00223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572693.1">
        <property type="nucleotide sequence ID" value="XM_003572645.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FLY3"/>
    <dbReference type="STRING" id="15368.A0A0Q3FLY3"/>
    <dbReference type="EnsemblPlants" id="KQK00223">
        <property type="protein sequence ID" value="KQK00223"/>
        <property type="gene ID" value="BRADI_3g48010v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829312"/>
    <dbReference type="Gramene" id="KQK00223">
        <property type="protein sequence ID" value="KQK00223"/>
        <property type="gene ID" value="BRADI_3g48010v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829312"/>
    <dbReference type="OrthoDB" id="637682at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FLY3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000008">
        <property type="entry name" value="Transcription termination factor MTERF5 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF9">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR MTERF5, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK00223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="484" mass="54652" checksum="26AB25835433E88B" modified="2016-01-20" version="1">MTSLQAAPRLRLHLAPCEPRGRLLPPSRLVLSPSSCRLYTLISRQLPICNAQSYADDLLVASAQSSTIARSRLLAAEREEAKAVLSLFLRQKGLRSAVAARIANKSDGFIEHLVSKLQIAYRSRYAEGRELSTPEIRDALLPYLEALSKEHGDGLFEVVENFPDPFAAERESLSYSMVLTPTSSNKQKAIARVSTPTSGGALPELVLYLLDFGMDHEEIKNIVRKFPAFAYYNVDRKIKPLVDLLLELGVPRSSIPGIIRKRPQLCGISLTDNLKPMMAYMENIGVNKAQWGKVLCRFPAFLTYSRQKVEVTVSYLTELGVSSENIGKILTRCPHLMSYSVNDNLRPTAEYFRSIGADAASLIQKCPQAFGLNIESKLKPITKFFLDREFSIEEIGIMVNRFGIIHTLSLEENLLPKYEYFLTMGYPRYELVKFPQYFGYSLEQRIKPRYARMTGCGVRLILNQMLSISDSRFQEILQKKMDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FNZ2</accession>
    <name>A0A0Q3FNZ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hexosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100840450</name>
        <name evidence="13" type="ORF">BRADI_3g45800v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014755766.1">
        <property type="nucleotide sequence ID" value="XM_014900280.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014755767.1">
        <property type="nucleotide sequence ID" value="XM_014900281.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014755769.1">
        <property type="nucleotide sequence ID" value="XM_014900283.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317073.1">
        <property type="nucleotide sequence ID" value="XM_024461305.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317074.1">
        <property type="nucleotide sequence ID" value="XM_024461306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FNZ2"/>
    <dbReference type="FunCoup" id="A0A0Q3FNZ2">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FNZ2"/>
    <dbReference type="EnsemblPlants" id="KQJ99881">
        <property type="protein sequence ID" value="KQJ99881"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99882">
        <property type="protein sequence ID" value="KQJ99882"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99883">
        <property type="protein sequence ID" value="KQJ99883"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99884">
        <property type="protein sequence ID" value="KQJ99884"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99885">
        <property type="protein sequence ID" value="KQJ99885"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68832">
        <property type="protein sequence ID" value="PNT68832"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68833">
        <property type="protein sequence ID" value="PNT68833"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840450"/>
    <dbReference type="Gramene" id="KQJ99881">
        <property type="protein sequence ID" value="KQJ99881"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99882">
        <property type="protein sequence ID" value="KQJ99882"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99883">
        <property type="protein sequence ID" value="KQJ99883"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99884">
        <property type="protein sequence ID" value="KQJ99884"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99885">
        <property type="protein sequence ID" value="KQJ99885"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68832">
        <property type="protein sequence ID" value="PNT68832"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68833">
        <property type="protein sequence ID" value="PNT68833"/>
        <property type="gene ID" value="BRADI_3g45800v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FNZ2">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045492">
        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000018">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ99884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="677" mass="75900" checksum="47A482F7F0A1A0BD" modified="2016-01-20" version="1">MESKIYRESNRGCTQAVSDISMRNRRRAWTVKTYACNSVSKKGYVGSCYTKHKPLSLLLPEGFSGKMLYVKLVLILLMCASFMGLLHSPSIRHGHGDDQHSTMSPEVSRQGSLAPADADEPADSGGYASSLRIDWSLISKAVQEAAARGVEGGGHGLRVGLLNFDGDEVEQWRTVLPESAAASAVHLERVGSNVTWEHLYPEWIDEEELYAAPTCPDLPEPQPAAEGAQYGYDIVAVKLPCSGASGWSKHVPRLHLQLAAARLASGRGSSAAHVVVVSPSRCFPAPNLFRCKDEAMHDGDVWLYRPDMAELRHKLALPVGSCKLAMPLKALLGEAHAHATAPQRREAFATILHSEQLYACGAIVAAQSIRTSSAPDVQRDMVALVDETISSRHRAALELAGWKVRTIRRIRNPRASPDAYNEWNYSKFWLWTLTDYSRVVFLDADLLVQRAMDPLFAMPELSATGNHGTLFNSGVMVIEPCNCTFSLLMSHIGDIGSYNGGDQGYLNEVFSWWHRLPSRANYMKHFWSGDSAERREAKRRVLAARPPVALAVHFVGMKPWFCFRDYDCNWNAAELRQFASDEAHARWWEAHDAMRPTSLRRFCLLDERQKALLRWDAMEARKANFSDGHWRERIVDPRRRICADDEAGLRCREREIEGRRVEGNRVTTSYAKLIHNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FZ71</accession>
    <name>A0A0Q3FZ71_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g15147v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FZ71"/>
    <dbReference type="EnsemblPlants" id="KQK04671">
        <property type="protein sequence ID" value="KQK04671"/>
        <property type="gene ID" value="BRADI_2g15147v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04671">
        <property type="protein sequence ID" value="KQK04671"/>
        <property type="gene ID" value="BRADI_2g15147v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FZ71"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK04671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="126" mass="12615" checksum="776C96F2172C49C5" modified="2016-01-20" version="1" fragment="single">GSGRRTSSPAAARAGGAGERLGRRGVARQAAGSRAARGAGARGGWPAAAAHGRGAEHGRAGTARAHGQAGARAARRRRGPATGGGATSGRRPALRKMAGAGGRRRRRRPNAKASASRRSGRGEERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GIE9</accession>
    <name>A0A0Q3GIE9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Acyl-[acyl-carrier-protein] desaturase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100846588</name>
        <name evidence="18" type="ORF">BRADI_2g56850v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="16">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the fatty acid desaturase type 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564771.2">
        <property type="nucleotide sequence ID" value="XM_003564723.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GIE9"/>
    <dbReference type="STRING" id="15368.A0A0Q3GIE9"/>
    <dbReference type="EnsemblPlants" id="KQK10887">
        <property type="protein sequence ID" value="KQK10887"/>
        <property type="gene ID" value="BRADI_2g56850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846588"/>
    <dbReference type="Gramene" id="KQK10887">
        <property type="protein sequence ID" value="KQK10887"/>
        <property type="gene ID" value="BRADI_2g56850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846588"/>
    <dbReference type="OrthoDB" id="1924153at2759"/>
    <dbReference type="UniPathway" id="UPA00199"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GIE9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045300">
        <property type="term" value="F:stearoyl-[ACP] desaturase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01050">
        <property type="entry name" value="Acyl_ACP_Desat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.620.20:FF:000002">
        <property type="entry name" value="Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005067">
        <property type="entry name" value="Fatty_acid_desaturase-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31155">
        <property type="entry name" value="ACYL- ACYL-CARRIER-PROTEIN DESATURASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31155:SF31">
        <property type="entry name" value="STEAROYL-[ACYL-CARRIER-PROTEIN] 9-DESATURASE 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03405">
        <property type="entry name" value="FA_desaturase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000346">
        <property type="entry name" value="Dlt9_acylACP_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="15" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000346-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK10887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="42587" checksum="B227A9BB5291F415" modified="2016-01-20" version="1">MQAHAIVRAPAHHHHLPPLSRHHCRPVSAAAVAPPPTQRRVTHSMPPEKAEVFRSLQGWATQSLLPLLKPVEDCWQPTDFLPDSSSEMFEHEARELRARAAGLPDELYVVLVGDMVTEEALPTYQTMINTLDGVRDETGASACPWAVWTRSWTAEENRHGDVLNKYLYLSGRVDMRMVEKTVQFLIGSGMDPRTENNPYLGFVYTSFQERATAVSHGNTARLAKAHGDDTLARACGTIAADEKRHETAYARIVEQLLRLDPDGAVLAVADMMRKRITMPAHLMRDGRDPGLFGHFAAVAQRLGVYTARDYADIVEFLVKRWKLEALEGGLSGEGRRARDYLCGLAPRMRRAAERAEDMAKKDEPRKIKFSWIFDREVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GT25</accession>
    <name>A0A0Q3GT25_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g13976v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GT25"/>
    <dbReference type="EnsemblPlants" id="KQK14032">
        <property type="protein sequence ID" value="KQK14032"/>
        <property type="gene ID" value="BRADI_1g13976v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14032">
        <property type="protein sequence ID" value="KQK14032"/>
        <property type="gene ID" value="BRADI_1g13976v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GT25"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK14032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="80" mass="9088" checksum="BE459FCBF1DA6759" modified="2016-01-20" version="1">MTDSRAIHAASYAKVFFLQSFYSRHAAGFRCAKCTAHLVAPDAFPLIIGHRKWIVFSSSSFFSSYFSCPHQRENLTCHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I264</accession>
    <name>A0A0Q3I264_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100842654</name>
        <name evidence="2" type="ORF">BRADI_3g11414v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571250.1">
        <property type="nucleotide sequence ID" value="XM_003571202.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I264"/>
    <dbReference type="STRING" id="15368.A0A0Q3I264"/>
    <dbReference type="EnsemblPlants" id="KQJ94603">
        <property type="protein sequence ID" value="KQJ94603"/>
        <property type="gene ID" value="BRADI_3g11414v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842654"/>
    <dbReference type="Gramene" id="KQJ94603">
        <property type="protein sequence ID" value="KQJ94603"/>
        <property type="gene ID" value="BRADI_3g11414v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842654"/>
    <dbReference type="OrthoDB" id="679141at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3I264">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34360:SF2">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34360">
        <property type="entry name" value="OS08G0519400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ94603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="376" mass="42947" checksum="BDD3617E1581B5AC" modified="2016-01-20" version="1">MSSSAATEATPLLAAAFLLRLRPIISPSAAAVVHGIRSAAALLAIAAFLAVICAVPAARSQHASDAEALRLEIDDLRLKIARLESILEGDTKSLRTKAYIMEEDNKLTEAMEHDIQLLMNVEETKKSERKSYSESNIFAMEDEVQILQQEVRKINSIAYTIESLANDAEKRVEFLSNEVKKIEDIIAEQWIQIRQFEQAFVLTKMMTSKVHERRLSGNAYYWPGKYTVPKYIRNLDLHGMFLVGASLTRTCFSHTYKHWRSFVQAMNRCYHEASRFCKAIRRPCPPDIHRPDVFFLGGSISRSCISLPYKQFKILVSSAQRIHHKVQVNLQDVMRSNRYSRGLANEMITFCMAYLLVISPVWIAWFIFSMRFGSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I5G1</accession>
    <name>A0A0Q3I5G1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100823789</name>
        <name evidence="5" type="ORF">BRADI_3g53837v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235903.1">
        <property type="nucleotide sequence ID" value="XM_010237601.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I5G1"/>
    <dbReference type="FunCoup" id="A0A0Q3I5G1">
        <property type="interactions" value="2195"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3I5G1"/>
    <dbReference type="EnsemblPlants" id="KQK01099">
        <property type="protein sequence ID" value="KQK01099"/>
        <property type="gene ID" value="BRADI_3g53837v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823789"/>
    <dbReference type="Gramene" id="KQK01099">
        <property type="protein sequence ID" value="KQK01099"/>
        <property type="gene ID" value="BRADI_3g53837v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823789"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3I5G1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06610">
        <property type="entry name" value="STKc_OSR1_SPAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000099">
        <property type="entry name" value="Serine/threonine-protein kinase BLUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000208">
        <property type="entry name" value="serine/threonine-protein kinase BLUS1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047173">
        <property type="entry name" value="STRAD_A/B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014:SF24">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE FRAY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="30"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="486"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK01099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="699" mass="77350" checksum="2E774DA32A9D19DD" modified="2016-01-20" version="1">MVGSGEKAGGGTGSGGGGERRKYPIGVEDYELYEEIGQGVSAIVYRALCKPLDEIVAVKVLDFERTNSDLNNIMREAQTMILIDHPNVVKALCSFTNNQTLWVVMPYMAGGSCLHIMKSVHPDGFEEAIIATVLREVLKGLEYLHHHGHIHRDVKAGNILVDSRCGVKLGDFGVSACLFESGDRQRARNTFVGTPCWMAPEVMEQLHGYDFKADIWSFGITALELAHGHAPFSKYPPMKVLLMTLQNAPPGLDYERDKKFSRHFKQMVAMCLVKDPSKRPTAKKLLKQPFFKQARSNDFISRKLLEGLPGLGARYQALKEKDEHLLAQKKMPDGKKEEISQDEYKRGISSWNFDIDDLKSQASLVTECEDIISSKDTNTSSIYDFDTILQEQALEGPLLSRGFSMKYDTDIESDVMANDKSTVSSPEQSVCPSRNASMRATPSEVLLNGHVRKQNSMECCDLDLQEKDSDAIPTTSFHERKFSFSSCSSDGFLSSKESSKPQISIHNRDKGNGGALQVSDEPSPEAAPKAPKSSVSNVEEHDDRSKPPLIQQRGRFKVTPGHVELDKAHSPGLQKCHSMQAISHLPSLSIPSSAEAASSIIGGSFYIQLYSVLQTNILQREQILHAMKQLIGSDLASPLCLPSISRLASPSSALSVDRSMLEAAHEKEKELLNEVLELQWRLLCSQDEVQRLKAKAAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I780</accession>
    <name>A0A0Q3I780_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7597 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_5g03715v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I780"/>
    <dbReference type="FunCoup" id="A0A0Q3I780">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ81904">
        <property type="protein sequence ID" value="KQJ81904"/>
        <property type="gene ID" value="BRADI_5g03715v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81904">
        <property type="protein sequence ID" value="KQJ81904"/>
        <property type="gene ID" value="BRADI_5g03715v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3I780"/>
    <dbReference type="OrthoDB" id="721783at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056018">
        <property type="entry name" value="DUF7597"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33075:SF10">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33075">
        <property type="entry name" value="OS02G0499800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24530">
        <property type="entry name" value="DUF7597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7597" evidence="2">
        <location>
            <begin position="166"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ81904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="405" mass="45554" checksum="AB78C7F953B76B23" modified="2016-01-20" version="1">MAVHASITLGLDFAPGVAFRKEVKRIHGVLVHPEGKSKHFYLAVSFRLALECCLGGLCDGFSVVHLRDQVFRFSVSTKSVGFMIYNLHKFSCAQFKCFFHLWGNGGPRWNSEFKSWQRENQEEWTLILPSDRYVNRALNAMNSVASPVLKSASRALLWIHANFDLDPAPYVPRGMAIEDGGPFCLQRTFVTLAGALARRHESYLIAMVEPPLPTEEVPAVIAQVHAYMVEILHYEVVSLHRHPSGLARFRMCNAIDRDMLVSLLAVDFGPGRTLRFVKHDEANNSLTTEFTRNGWVMLLGIPLDLQDDAFIMQAVETFGKLEYWIQRESTDVRVIAKVIYEDATTVPRDIVVREVMVVGGRTVSWTIPVYILNSGFADEFPPPEDQLPSHGGNQHLLPGHGPVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3INI3</accession>
    <name>A0A0Q3INI3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
            <ecNumber evidence="10">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100846804</name>
        <name evidence="11" type="ORF">BRADI_2g35197v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Carboxypeptidase II is a dimer, where each monomer is composed of two chains linked by a disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566533.2">
        <property type="nucleotide sequence ID" value="XM_003566485.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3INI3"/>
    <dbReference type="FunCoup" id="A0A0Q3INI3">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK07419">
        <property type="protein sequence ID" value="KQK07419"/>
        <property type="gene ID" value="BRADI_2g35197v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846804"/>
    <dbReference type="Gramene" id="KQK07419">
        <property type="protein sequence ID" value="KQK07419"/>
        <property type="gene ID" value="BRADI_2g35197v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846804"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3INI3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000003">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000013">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF198">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043073437" description="Carboxypeptidase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK07419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="466" mass="51843" checksum="9693314CC0BB55BD" modified="2016-01-20" version="1">MKTMTGGHYPLALALALAALLALAAAASEDHAADRIARLPGQPAVDFDMYSGYITVDKAAGRSLFYLLQEAPEEAQPAPLVLWLNGGPGCSSVAYGASEELGAFRIRPDGANLVLNQYRWNKVANILFLDSPAGVGFSYTNTSSDLYTSGDNRTAHDSYTFLANWFEKFPHYKYRDFYIAGESYAGHYVPELSQLVYRKNKGMSKPIINFKGFMVGNGLIDDYHDYVGTFESWWNHGLISDDTYRLLKASCLHDSFIHPSPACNAAQDTAATEQGNIDMYSLYTPVCNQTASVSRPRPRGRYPWMSGSYDPCTERYSTVYYNRPEVQRALHANVTGINYTWATCSDTINKNWGDAPRSMLPIYKELIQAGLRIWVFSGDTDAVVPLTATRYSIDALDLPTTIGWYPWSDSKEVGGWSQVYKGLTLVTIRGAGHEVPLHRPRQALIMFQNFLRGMPLPRQTSNGTMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IXE2</accession>
    <name>A0A0Q3IXE2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF4005 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100831725</name>
        <name evidence="4" type="ORF">BRADI_2g18220v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565979.1">
        <property type="nucleotide sequence ID" value="XM_003565931.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IXE2"/>
    <dbReference type="STRING" id="15368.A0A0Q3IXE2"/>
    <dbReference type="EnsemblPlants" id="KQK05132">
        <property type="protein sequence ID" value="KQK05132"/>
        <property type="gene ID" value="BRADI_2g18220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831725"/>
    <dbReference type="Gramene" id="KQK05132">
        <property type="protein sequence ID" value="KQK05132"/>
        <property type="gene ID" value="BRADI_2g18220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831725"/>
    <dbReference type="OrthoDB" id="671489at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IXE2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF34">
        <property type="entry name" value="OS05G0541100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="17"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK05132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="45620" checksum="AA4B3F9DD3F21E5B" modified="2016-01-20" version="1">MGASGKWIKSLVGLKAPPSSAAAGTKGRKWSRLWRSPSSSRGGGNNAPSPWCETTSASSAAAAGALSSDVVAAVVRAPPRDFLVIRQEWAAVRVQTAFRAFLARRALKALRGIVRLQALVRGRLVRRQLAVTLNRMEALLRVQERAMERRARCCADGGDDPVREAEEQWCARQGSVDEVRSKMQMKHEGAVKRERAMAYSLSHQPRSVKHRGRPSSPASSLRNHESYIEGWMATKPWDSRRMDPNRSESHCLENSNELNLAGSKFSDSGTGRSVKVRRNNVATMVEAKSPSLLSASSASLDLAFDESSLSTSSVTPASAAMASEARSVDSGYRGGGPGYMSLTKSARARLDGCGGSRRGLSPQMQRQRSGGMPYRRRVALSSLDSQSNAGSDISAACRRLNSMSLKGPSMMTRSLDKENDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IY09</accession>
    <name>A0A0Q3IY09_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pectinesterase</fullName>
            <ecNumber evidence="3 8">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100824585</name>
        <name evidence="11" type="ORF">BRADI_2g19420v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pectinesterase family.</text>
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    <dbReference type="EC" id="3.1.1.11" evidence="3 8"/>
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        <property type="protein sequence ID" value="KQK05324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003565975.4"/>
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    <dbReference type="AlphaFoldDB" id="A0A0Q3IY09"/>
    <dbReference type="STRING" id="15368.A0A0Q3IY09"/>
    <dbReference type="EnsemblPlants" id="KQK05324">
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        <property type="gene ID" value="BRADI_2g19420v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT70886">
        <property type="protein sequence ID" value="PNT70886"/>
        <property type="gene ID" value="BRADI_2g19420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824585"/>
    <dbReference type="Gramene" id="KQK05324">
        <property type="protein sequence ID" value="KQK05324"/>
        <property type="gene ID" value="BRADI_2g19420v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT70886"/>
        <property type="gene ID" value="BRADI_2g19420v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824585"/>
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        <property type="reaction ID" value="UER00823"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IY09">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000018">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF57">
        <property type="entry name" value="PECTINESTERASE 53-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005965272" description="Pectinesterase" evidence="8">
        <location>
            <begin position="27"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase catalytic" evidence="10">
        <location>
            <begin position="117"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="27"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK05324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JAU5</accession>
    <name>A0A0Q3JAU5_BRADI</name>
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        <recommendedName>
            <fullName evidence="2">Protein ENHANCED DISEASE RESISTANCE 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">BRADI_3g17060v3</name>
    </gene>
    <organism evidence="3">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JAU5"/>
    <dbReference type="EnsemblPlants" id="KQJ95416">
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        <property type="gene ID" value="BRADI_3g17060v3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="Pfam" id="PF07059"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ95416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="376" mass="41388" checksum="52CFBA771A58EE95" modified="2016-01-20" version="1">MGKLIRLEKITTLFGLRARCGLVLADGAVDLCLDSLGHLILLCHWLIGFALSSISVVEELIEKKMTSSDDEAEHQWIENVKSGGAVLCQPPENCPNGWGTPAGDKFMVRGPDYFITKAKIPGGEYLLKPLGFDWIRGPTKICEVLKNKNHRVRKAIDEEVSHGNQPFVWAFNLQLPSKENYSAVFYFVSPKPAPEGSLMDQFLKGDDAFRKSRLKLIANVVKGPWIVRTAVGEQAICILGRALSCKYVQGSNFIEVDVDIGSSIVANAIVHLAFGYVTTLTVDLAFLIESQAESELPERLLGAVRFSELSPGSAGVYEVPSEEQQESAPFLPTRLWQGFSQLLQNPGNSRETPPSSQNTNGNLHEEGADENTNRYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JQ95</accession>
    <name>A0A0Q3JQ95_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase II subunit RPB9-like zinc ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100840316</name>
        <name evidence="3" type="ORF">BRADI_3g51447v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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            <taxon>Poaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003570032.4"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3JQ95"/>
    <dbReference type="EnsemblPlants" id="KQK00729">
        <property type="protein sequence ID" value="KQK00729"/>
        <property type="gene ID" value="BRADI_3g51447v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840316"/>
    <dbReference type="Gramene" id="KQK00729">
        <property type="protein sequence ID" value="KQK00729"/>
        <property type="gene ID" value="BRADI_3g51447v3"/>
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    <dbReference type="OrthoDB" id="282152at2759"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="expression patterns" value="baseline"/>
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        <property type="term" value="C:RNA polymerase III complex"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
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        <property type="project" value="InterPro"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK00729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="95" mass="10316" checksum="1A10000B289E04AE" modified="2016-01-20" version="1">MEFCPACGMLLQIQPATGGHRLRLFCPTCQYVCPIKHKIVKKAKLVKKELESTESASKTDGEPKESASKTDGEPKQSAPKTDGEPKQSAPKIDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K8A4</accession>
    <name>A0A0Q3K8A4_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC100838777</name>
        <name evidence="1" type="ORF">BRADI_2g33000v3</name>
    </gene>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_2g33000v3"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS05G0200700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK07089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="222" mass="23929" checksum="27F58D1699DC8A63" modified="2016-01-20" version="1">MDLARTPARRPMEPGLARRLWHVVLAVCHMLRRGLGRKRLMMDLHLLLGRGKLAGRALRGHIASSSSSSSALAWRPREVEFSCTTTPSFPQHYGGLFPFNFKGRGGAGARGGREYGGLDAAAVARAFEMMSADVESGRATPAAVAGGVSAAATPSPMVAWILGRSPAGVRPLRVTDSPFPVVPEDGAGSGNERVNAEAEDFIRRFYEQLRMQHPSVATPEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KU78</accession>
    <name>A0A0Q3KU78_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100833770</name>
        <name evidence="14" type="ORF">BRADI_5g16817v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581459.2">
        <property type="nucleotide sequence ID" value="XM_003581411.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KU78"/>
    <dbReference type="STRING" id="15368.A0A0Q3KU78"/>
    <dbReference type="EnsemblPlants" id="KQJ83781">
        <property type="protein sequence ID" value="KQJ83781"/>
        <property type="gene ID" value="BRADI_5g16817v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833770"/>
    <dbReference type="Gramene" id="KQJ83781">
        <property type="protein sequence ID" value="KQJ83781"/>
        <property type="gene ID" value="BRADI_5g16817v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833770"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KU78">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000489">
        <property type="entry name" value="Inactive receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000101">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46084:SF19">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46084">
        <property type="entry name" value="PROTEIN MALE DISCOVERER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="334"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="408"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ83781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="709" mass="78197" checksum="9B0255B95F551399" modified="2016-01-20" version="1">MAEQSCRPIGHHRRWLLGVAVKCNFRGCCFFPPRDQVNTSLPCEQSLLLFCSNSTMEKLLEVSRRLVMLLGCVLLIAPSGSGSSVLDDVAALLAFKKAIIEDPLSKLSDWNPTEPDPCAWSGVTCSPDNRVEILNLSSSSLTGFLAPDIGSLSSLQKLTLDNNTLVGSIPREIGKLKNLTVLDLSTNQLVGPIPREIGDMQKTTKIDLHVNWLNGAIPPELVKLTNLVELRLSNNSLTGTIPASNDSIMVSTNREDQIGLCRLSQLTDIDLSYNFLDGDVPTCLRKIERSSMVGNCFQNNDIINRPVQQCENSKDGDKDNTIGGSGQKSLLQPLWLLILEVLTGISLLTILSLCVITFLRRRNARSSGNSVPWTRAISWKENTVISIDDDFLGNVPKISRQELAEACEDFSNIIGSSHETVVYKGTMKDGREIAVVSLSVSVHYWTNYIELYFQKEVVEVARLSHENVAKMVGYSKDSNPFSRMLVFEYPANGTLYEHLHDGEGCQLSWPRRMKIALSIARVLRHLHTELQPPFAVATLTSSSVYLTEDFSPKIIDFERWRALVAKPVFGNGCVVNGNGGPFNGIMDSRHIRFMDVQANTFAFGVILLELISGKASLSKDTGDLLDWAREHLDQPEEFSKLVDPKLQSVSQENLGIICNAVNLCIDSEPSRRPSMNMIAAILEEGVDTSTATALRSSSLAWAQAELAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LVW1</accession>
    <name>A0A0Q3LVW1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100841852</name>
        <name evidence="3" type="ORF">BRADI_3g23105v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756618.1">
        <property type="nucleotide sequence ID" value="XM_014901132.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LVW1"/>
    <dbReference type="EnsemblPlants" id="KQJ96437">
        <property type="protein sequence ID" value="KQJ96437"/>
        <property type="gene ID" value="BRADI_3g23105v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841852"/>
    <dbReference type="Gramene" id="KQJ96437">
        <property type="protein sequence ID" value="KQJ96437"/>
        <property type="gene ID" value="BRADI_3g23105v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1922499at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LVW1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="2">
        <location>
            <begin position="632"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ96437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="667" mass="73666" checksum="8E7B6794F7EBB8B9" modified="2016-01-20" version="1">MFRNKVLVRAIPSSAPPPPFLLRQVSFSAAAPSLDLDILCCRLSRSHRLACLPQPPTDALVIYQRCQSRAADEVVSKIAAAFPIASVGEEEEVVCSGSLVAKAIECGLRCLMLEHGWSFVGESIYVETMFAASEERTDLCALNVEVRSGPNDHFDFIVSPDAFRFTAHKISDIASSSMMETFEHSKEVSLDGCNLQTACAILPTLKECHVIAFSKSLPPGHSLDKFIEFCSIKHGLETNYSYHAAVKLTHGDSWEKQWLPSSFILQGSALQPALKSVRALKAMSSLRSFIEFLKAWNFFGQHQLVIKEQLILNCATTLPSWKKAITVHTARADYSEDFGLSRPHLKEKLLTLDFRTPKPAVFCSLSAKLGNTKVDEKALSSDDDGTGNGKGYTGHGFQSQPIVLTSSFKSHVALLKPSFSRPKRADTNNTRHFSVESSDADSLNKLSDTSLPKSSLRCFTKATHANPVNSSSASNIKQVIQEVQEGHQGGDHARNFKASEYLKRKHAEFLGNSGEGGNVKDYIPDVLQETRSMPDIQEDFLSTKAIQPKSKSMGCKGKMTATTKLKRKPEVVTNEFNKKVVDHQKDEFSKKITRVKVKAKDELTSTRTKARLDVDKDELMAKVIDHHKRGELHLLTVAELKGFLSTKKAKVGGSKEVLIQRASELLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3MQI6</accession>
    <name>A0A0Q3MQI6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g26928v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06550.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3MQI6"/>
    <dbReference type="EnsemblPlants" id="KQK06550">
        <property type="protein sequence ID" value="KQK06550"/>
        <property type="gene ID" value="BRADI_2g26928v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06550">
        <property type="protein sequence ID" value="KQK06550"/>
        <property type="gene ID" value="BRADI_2g26928v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3MQI6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06550.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="160" mass="17482" checksum="604EA808E1485AA3" modified="2018-03-28" version="2">MRGRPLVRTASARRFPPPVHSRAPSTPTCRQVRLCAAHTSRPATGRRAPLPVRNRHPGLRLVLLTSKSRGEPPARTSRGGGGQSAQRSSGSAAAFRTQTPPTCTSWWRRQERRRARSEGRRRVPDRAGGGGEIRRRGGGATRALLVFLFSFLGGTCFFCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NCQ0</accession>
    <name>A0A0Q3NCQ0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g18476v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NCQ0"/>
    <dbReference type="EnsemblPlants" id="KQK14766">
        <property type="protein sequence ID" value="KQK14766"/>
        <property type="gene ID" value="BRADI_1g18476v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14766">
        <property type="protein sequence ID" value="KQK14766"/>
        <property type="gene ID" value="BRADI_1g18476v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NCQ0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK14766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="73" mass="7739" checksum="70B0D2B0772B2D60" modified="2016-01-20" version="1">MSILICVQSSGSVDCQSVSVSQFVVCVPSSDIATVCMRCMDARWKGGRSRSAGLSEEISSGWGMCLCIIKCVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3Q0K0</accession>
    <name>A0A0Q3Q0K0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lebercilin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100831555</name>
        <name evidence="2" type="ORF">BRADI_3g15580v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3Q0K0"/>
    <dbReference type="EnsemblPlants" id="KQJ95169">
        <property type="protein sequence ID" value="KQJ95169"/>
        <property type="gene ID" value="BRADI_3g15580v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g15580v3"/>
    </dbReference>
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    </dbReference>
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        </location>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ95169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
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    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CF43"/>
    <dbReference type="EnsemblPlants" id="PNT60647">
        <property type="protein sequence ID" value="PNT60647"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="691957at2759"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT60647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10441" checksum="02D5EEF3E59C7250" modified="2018-03-28" version="1">MVVGNGSSTLFWEDRWMDGRAISKLAPALYQLISKRTCKSRNVNEALADQRWIRDIRGALGPVALWQYIQIRNLVRDVLLTDAADVLQIWI</sequence>
</entry>
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    <accession>A0A2K2CJP7</accession>
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        <name evidence="2" type="ORF">BRADI_4g00224v3</name>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT62250">
        <property type="protein sequence ID" value="PNT62250"/>
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    </dbReference>
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        <property type="gene ID" value="BRADI_4g00224v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CJP7"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT62250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62250"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="51" mass="5921" checksum="2B8A5E6586939D67" modified="2018-03-28" version="1">MHTKPARRSSGVTQGMMSRRRARRREWRDTNCGARLQCCVQDMLQVICIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CRF8</accession>
    <name>A0A2K2CRF8_BRADI</name>
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            <fullName evidence="1 2">Uncharacterized protein</fullName>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT64616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CRF8"/>
    <dbReference type="EnsemblPlants" id="PNT64616">
        <property type="protein sequence ID" value="PNT64616"/>
        <property type="gene ID" value="BRADI_4g30613v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64616">
        <property type="protein sequence ID" value="PNT64616"/>
        <property type="gene ID" value="BRADI_4g30613v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CRF8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT64616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="95" mass="11044" checksum="D3B63B23894A7609" modified="2018-03-28" version="1">MQHMCGRMIQQVLFDLLSVIRISDLCKLVLKTYFLMSQEETSFIQDLLLLTLFATCCSQEHLACLQMIKFHCLLPSDDQSKFAKKQRGGQKKEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CWC1</accession>
    <name>A0A2K2CWC1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial ribonuclease P catalytic subunit</fullName>
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        </recommendedName>
        <alternativeName>
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        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100845397</name>
        <name evidence="20" type="ORF">BRADI_3g10160v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
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    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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            <strain evidence="21">cv. Bd21</strain>
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    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
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        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
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        </reaction>
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    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
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        </cofactor>
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        <text evidence="3">Belongs to the PPR family. P subfamily.</text>
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        <property type="gene ID" value="BRADI_3g10160v3"/>
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        <property type="gene ID" value="BRADI_3g10160v3"/>
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        <property type="protein sequence ID" value="PNT66323"/>
        <property type="gene ID" value="BRADI_3g10160v3"/>
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        <property type="gene ID" value="BRADI_3g10160v3"/>
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        <property type="evidence" value="ECO:0000318"/>
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    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
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    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
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        <location>
            <begin position="180"/>
            <end position="236"/>
        </location>
    </feature>
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        <location>
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            <end position="443"/>
        </location>
    </feature>
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        <location>
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            <end position="390"/>
        </location>
    </feature>
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            <end position="713"/>
        </location>
    </feature>
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        </location>
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        </location>
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            <end position="124"/>
        </location>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
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    </dbReference>
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        <property type="gene ID" value="BRADI_3g16657v3"/>
    </dbReference>
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        </location>
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            <begin position="31"/>
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        </location>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Bd21</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
    <sequence length="356" mass="39775" checksum="3088C673BC9A54D8" modified="2018-03-28" version="1">MPKGAASPGARKNRKDMGRAWLKKIKKGSPTATRLLLTRRRARRSRRLTLSTSFSLKPLEEAIAHWSDEMKEAVGSANGLAGLFKQRYIGLLDRKSALYLLFHLDPDTMELVMGDGLHDRIPITVEVIGHILGITAVGDRLVLGDRVNHAELRKRTNQLLQYSDDSSRSVPVRRIFHLLKDANMGLLEGPGFHRIVTGHTLLAFATVLGPRRAQASVSDEALEVVSETAQINKYNMALFVLEGLKVAAYKVRDEMPSNPSSIELEGCVIALQIALVEHRRFGLRGLFHEKKPRIEDYIETKGDLKACLPSNIEIRQYDEIVDEQQDLPASDERSACHRAPPDAEGSSSKHQRKARL</sequence>
</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
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            <dbReference type="EMBL" id="PNT68807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
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        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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            <begin position="21"/>
            <end position="38"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
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    </feature>
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        <source>
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    </evidence>
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</entry>
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    <reference evidence="2 3" key="1">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Rokhsar D."/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT69281.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="252" mass="29331" checksum="EC9142467085569B" modified="2018-03-28" version="1" fragment="single">MIVCLSDLFHANSLNFICLQETKKKDYKQCFFRRLDPGNDFFWKWVPSRGRSGGMLCGVRNESFEVSTCKLGNYVLQLNLWDKKKKERWSLLTVYGAAHPEFREEFLAELAAFCNAVDCPFIVGGDFNILRHAGKKNIMFTPSHSSALFNTIIHSLSLREIYMNGGLYTWSNNHEQPILERLDKVLMSTCWEGLFPLVSVTKLVRDKSDHCPLLLNTNENCTPKTHRDFHFDLSWLQKEDFLPKVEEIWQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <strain evidence="8">Bd21</strain>
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        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A2K2D604"/>
    <dbReference type="EnsemblPlants" id="PNT69714">
        <property type="protein sequence ID" value="PNT69714"/>
        <property type="gene ID" value="BRADI_3g60352v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583882"/>
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        <property type="gene ID" value="BRADI_3g60352v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583882"/>
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        </location>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT69714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="251" mass="25490" checksum="02A4FF56E3D07BA8" modified="2018-03-28" version="1">MTTRPDGGGGAAEKQMVPVAAAANGNGALAVRKAPSKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGSGTTPASFSTSSPSSLRSSNSTHSHSAAQAQPLGAGGAHQHHHLLPHAAPFILGKRVRGGEDDEDGHGNDQAAAVSMGPAPGFWALPARAADFGQLWSFAAAPEMMVAAAAPAMAGEASAARVGNYLPMGQGVNLIASLGGPGPAAGPPAGRAEEEAAR</sequence>
</entry>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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    <reference evidence="2 3" key="1">
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            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
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                <person name="Bevan M.W."/>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="Proteomes" id="UP000008810">
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    <proteinExistence type="predicted"/>
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            <end position="31"/>
        </location>
    </feature>
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            <begin position="17"/>
            <end position="29"/>
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    </feature>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT70256"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="42" mass="5174" checksum="95A66A9AB3BFFE44" modified="2018-03-28" version="1">MMLNQDMIGERNQPWKFKKETLKDERPGSYRDSFIPQQYGFY</sequence>
</entry>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA glycosylase family. DEMETER subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754549.1">
        <property type="nucleotide sequence ID" value="XM_014899063.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A2K2DA58">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT71156">
        <property type="protein sequence ID" value="PNT71156"/>
        <property type="gene ID" value="BRADI_2g23797v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844361"/>
    <dbReference type="Gramene" id="PNT71156">
        <property type="protein sequence ID" value="PNT71156"/>
        <property type="gene ID" value="BRADI_2g23797v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844361"/>
    <dbReference type="OrthoDB" id="5607at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DA58">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051747">
        <property type="term" value="F:cytosine C-5 DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141166">
        <property type="term" value="P:chromosomal 5-methylcytosine DNA demethylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000004">
        <property type="entry name" value="DNA glycosylase/AP lyase ROS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044811">
        <property type="entry name" value="DME/ROS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028925">
        <property type="entry name" value="RRM_DME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46213:SF2">
        <property type="entry name" value="PROTEIN ROS1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46213">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR DEMETER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15628">
        <property type="entry name" value="RRM_DME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="10">
        <location>
            <begin position="1253"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="132"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="192"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="331"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="565"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1180"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1787"/>
            <end position="1807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="566"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="580"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1795"/>
            <end position="1807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT71156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1807" mass="199256" checksum="4073645C2B594D7F" modified="2018-03-28" version="1">MSVRGDRSGSPANPSSVVLEARMDNLQANPAENQEIWTPAMAERRSTPSPSIPGNRSLFLGDGAPSIGCQNLAAGSSPITGPNLLSGCSRSMMSATHEGSASAPRAALQGSCNLPAGCTKVPISIFVFQRRRAGRGSRPPTPLSGTSIPAPALPGVPGDSEDAPSILLPRSDFLSIGRASNDVASDMAMNPSAQATPVSCSSEHPSIHFQPNGLDAESPKTGIQGNDSQPSSSSAVVTSGVHSKLESAIPARTDETQGQHTKQLIELLGEGVTDHNMLVYQRTPKKPRTQLKQNDPTVVATPAALKERTLTQIDIQISGTAKVETFQIEDTPARKVKPRRKKHRAKVIREDKRGKKQNSAVTTPEEKSPNQKARRSYVRKKRNLSSLEKCPGPVTDRSISGGTENAARSRIASVRRRLQFELGEQGVQGDQSSTGNSCQHNEKLAHAKSSLCSMTRSAVQIGHGLQANMENLPGGLAFGMTRKLNELLDEYIHLPEITPKPTQEISPATSGCLSREVTGKQDNAGSTHDPDATRMGVTIMEGNNKDLGVKYSNIDGFDMHRLATSIPETESTESQVTKVSKLEEKEHGQHTESDSSLTNSRDSIILRAATEMLAFCQAGGIKKKRSIRARRISFVPIMDIEKNKSQAFARLPQSCMEALYESSCIKFATKKRSQKERLHSPSSIQPNMDKKSMFSSRSIFSGGSNGLKGSEGTFQQTLPQAPDNRRINLDIYCEVPERSSEHTYMDHLQGVTSRLKYLDLNTEHLHRTEMLPSQTMSAVVSFGETGGLSNSLVPYSGQMIFPYERPLHLVKKQRPRAKVNLDYETTRVWNLLMGKATEPVDGTDVDKERWWQQEREVFQGRANSFIARMRLVQGDRHFSPWKGSVVDSVVGVFLTQNVSDHLSSSAFMALAASFPPGSVHSNCEDDITSQANEEIFSMSAVGGDISMFDFFDSGTRSDLEATEGFCIHQETEIDHRAHQFPDFSSIELTASAGSPPEIQSQKDMSSTESVMISETITETQLSSSSGKNYAPRYFVGGVNGAAFQQLGSNFDGNPLAGNDAATNELGCQRIIGAINDNGVGEHVIPSPSVLMYPFISVDNRQPDVANEPYVYSTSHKSSAGSASSHPKNGAVEKELPLFMPSDNHIAQINYSKTVISTLTTPETSTDLPVKLRYNKRAGFEASELQQHESHSVTGGNGMIADTASGADESTLKSGFIAHNGVPDKAAQASRPKKARTTNKKNMEKFDWDKFRRQACDDGHMNERTFERRDSVDWEAVRCADVQRISHAIRERGMNNVLAERIQSFLNRLVRDHGSIDLEWLRDIPPDSAKDYLLSIRGLGLKSVECVRLLTLHHLAFPVDTNVGRICVRLGWVPIQPLPESLQLHLLELYPVLETIQKYIWPRLCKLDQQTLYELHYQMITFGKVFCTKSKPNCNACPMRSECKHFASAFASARLALPAPQEKSLVKSSNQFSFENSGLPTRNHAWNSTVLSVLPQLEGSTYGRDVLATYSEPIVEEPASPREEECPETLENDIEDYDADNGEIPTIKLNMEAFAQNLENCIKESNKDLQSDDIAKALVAISTEAASIPVPKLKNVRRLRTEHYVYELPDSHSLVQQLELARREPDDPSPYLLTIWMEDDIKEMSKAPKSCCDSQMEADFCNNGKCHYCVPERENQSRYVRGTILVPCRTAMKGSFPLNGTYFQVNEVFADHKSSHDPIHVAREQLWSLQRRMVYFGTSVSTIFKGLTTEEVQQCFWRGFVCVRGFDMETKAPRPLCPHLHLAASKLPRSRKTAETEQNSGLAKASIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DAH6</accession>
    <name>A0A2K2DAH6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g25656v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DAH6"/>
    <dbReference type="EnsemblPlants" id="PNT71281">
        <property type="protein sequence ID" value="PNT71281"/>
        <property type="gene ID" value="BRADI_2g25656v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71281">
        <property type="protein sequence ID" value="PNT71281"/>
        <property type="gene ID" value="BRADI_2g25656v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DAH6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT71281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="102" mass="10661" checksum="0DA1284EF464DC72" modified="2018-03-28" version="1">MGVAATSILSGIRSSPTSINLLPLLGCNAGSSTCIYLLPLLRRDAGSSTGINLLPLLRRDAGSSTGINLLPLLGCYHTSSSFITFPSCSIFLFSRIVSCIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DBQ0</accession>
    <name>A0A2K2DBQ0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">BRADI_2g34314v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DBQ0"/>
    <dbReference type="EnsemblPlants" id="PNT71714">
        <property type="protein sequence ID" value="PNT71714"/>
        <property type="gene ID" value="BRADI_2g34314v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71714">
        <property type="protein sequence ID" value="PNT71714"/>
        <property type="gene ID" value="BRADI_2g34314v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DBQ0"/>
    <dbReference type="OrthoDB" id="680672at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF102">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="263"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="PNT71714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="590" mass="65170" checksum="0FF8346C41693CE1" modified="2018-03-28" version="1">MAERRASSLGVIMAVAMVVIMAVLGLGLVAAEEDPWPDAPRYKVEALVAIRAALRDPNGVLVDWVANSSVHPCNWTGVVCSVSLGIDLHSRNLSGTLSPEIGKIRWLEDVNLGDNDISGPIPETLGEFQSLVRVDLSNNRFSGTIPPALCKEPIYDLQLSHNNLSGTIPDAIFTHRSNFFVDLSFNNLSGTLPDYNISFYGINTANFEGNPILHYNCNGTCGSTPMQENALPKESPNKNESEACFGHLKRYMLKEIKQATNNFNRNNILGQGGFGIVYKGLLHDGTIAAVKRLKDFVSSTGEHQFRTEVAVISLVVHRNLLSLIGFCSEKNERLLVYPYMPNGTVSSKLQEYVNQKPALDWPTRKKIALGTARGLVYLHDQCYPKIIHRDIKASNVLLDEEFEAIVADFGMAKMLEQGQTHVISEIRGTFGRIAPEYLRTGESSEKTDVYAYGLLLMELITGRRTLDVREEEYPKGGLVDWARELLEEGQLSSLVDKRLGSDYDSAELVEMVQTVLLCAMYNADHRPRMSEVVRMLEGDGSSAKRWEALKDIPTTPLPGTPVFIPSLAHGGEGEEYQSGDIEAIELSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DCA1</accession>
    <name>A0A2K2DCA1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g37276v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DCA1"/>
    <dbReference type="EnsemblPlants" id="PNT71910">
        <property type="protein sequence ID" value="PNT71910"/>
        <property type="gene ID" value="BRADI_2g37276v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71910">
        <property type="protein sequence ID" value="PNT71910"/>
        <property type="gene ID" value="BRADI_2g37276v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DCA1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT71910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="68" mass="7847" checksum="F4640485D588C8C2" modified="2018-03-28" version="1">MEELMPQAKVILHESTPPVTCRSPRPHFSRVRLHVLPLCRQWVDLLQEQWGMKRSAVVVCGEILVSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DCT6</accession>
    <name>A0A2K2DCT6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g39277v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DCT6"/>
    <dbReference type="EnsemblPlants" id="PNT72096">
        <property type="protein sequence ID" value="PNT72096"/>
        <property type="gene ID" value="BRADI_2g39277v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72096">
        <property type="protein sequence ID" value="PNT72096"/>
        <property type="gene ID" value="BRADI_2g39277v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DCT6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT72096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="59" mass="7126" checksum="557E27899DD31E18" modified="2018-03-28" version="1">MERDDGGPRSQRPCRLRQRIIRDLPWLRRGTRPRCPSRLIQPRRGLCPRPLVEFEYIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DFI1</accession>
    <name>A0A2K2DFI1_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g52298v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DFI1"/>
    <dbReference type="EnsemblPlants" id="PNT73026">
        <property type="protein sequence ID" value="PNT73026"/>
        <property type="gene ID" value="BRADI_2g52298v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73027">
        <property type="protein sequence ID" value="PNT73027"/>
        <property type="gene ID" value="BRADI_2g52298v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73026">
        <property type="protein sequence ID" value="PNT73026"/>
        <property type="gene ID" value="BRADI_2g52298v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73027">
        <property type="protein sequence ID" value="PNT73027"/>
        <property type="gene ID" value="BRADI_2g52298v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DFI1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT73027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="140" mass="14802" checksum="5E1277499E6B8A4A" modified="2018-03-28" version="1" fragment="single">SFPCVSLPYPSHPRQQPPHVAVVACPSPTPLPQRRLLPPSSSLLTAALQIGGAAPSSPRYSPSAAEALRLPRQSRCSSAAKALPPPPPTPSWTPPSPTCLPQPTQFPSRPPQSPLAPATPPLLLPGYHLVWQPHELRPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DJ32</accession>
    <name>A0A2K2DJ32_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g12063v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DJ32"/>
    <dbReference type="STRING" id="15368.A0A2K2DJ32"/>
    <dbReference type="EnsemblPlants" id="PNT74287">
        <property type="protein sequence ID" value="PNT74287"/>
        <property type="gene ID" value="BRADI_1g12063v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74287">
        <property type="protein sequence ID" value="PNT74287"/>
        <property type="gene ID" value="BRADI_1g12063v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DJ32"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35545">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35545:SF22">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT74287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="41221" checksum="3536F882735DBE4E" modified="2018-03-28" version="1">MAARKKKDLPSRRQRRQACGEDRLSALSNDLLLQILRRVNSRTALSRRLAHLPRELPVLDLKVTDALPPRYRRRLLLLREARRTHQLCYFGSNMVTDITGRYERRATRSMVASVKSLLASGASRRATRLSFEVFAMPGGHQFLGHPGSGACRHAHGPDRAQAAGLQFPPWYQHQRKASRVSLAMPQAHQLLAAVTRGPHSAHHARLARLAQVNARPCLPPPQLQVLHLISCRYPLTGKSLRLVFNAPNSQIRELVIDSPLIIVELSSLPKLQSLTVLHSSFVLSSATAAPCLTHVSFVFSVGPLEGDSLSFHYRNNLDRIFRVLMSFFERAIGMTDLALWFTGPGLQTTIKERSQPLLQVLLLLHVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKR6</accession>
    <name>A0A2K2DKR6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC112269938</name>
        <name evidence="2" type="ORF">BRADI_1g23290v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313236.1">
        <property type="nucleotide sequence ID" value="XM_024457468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DKR6"/>
    <dbReference type="EnsemblPlants" id="PNT74861">
        <property type="protein sequence ID" value="PNT74861"/>
        <property type="gene ID" value="BRADI_1g23290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112269938"/>
    <dbReference type="Gramene" id="PNT74861">
        <property type="protein sequence ID" value="PNT74861"/>
        <property type="gene ID" value="BRADI_1g23290v3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DKR6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT74861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="166" mass="18172" checksum="6E28A7AF01923EDC" modified="2018-03-28" version="1">MGDVIFPVTSADMESLESVTAEPANQPLNAPPSTAQWGWGERLRTLRLTARSHTGRRHQRGGAPAPSGGIAIGLEQALADRHRMEMQIVANKESFRSASSRLPRVDSDSSEKGVRLIQKALRESRVPFQADAARQGEAASESEDSGEKLGPFGMFSARLMFKFKMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DN72</accession>
    <name>A0A2K2DN72_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g37307v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DN72"/>
    <dbReference type="EnsemblPlants" id="PNT75719">
        <property type="protein sequence ID" value="PNT75719"/>
        <property type="gene ID" value="BRADI_1g37307v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75719">
        <property type="protein sequence ID" value="PNT75719"/>
        <property type="gene ID" value="BRADI_1g37307v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DN72"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT75719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="10171" checksum="C4A6DA85AEC3C6DB" modified="2018-03-28" version="1">MELNHRPSVLYIYNINPKKKSNTRRTYATPPRRFVQDLESDLVFSPPTSTPHLPPSQCHHPPLSCVSTSGCELDETFDVMMNSARFHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQJ5</accession>
    <name>A0A2K2DQJ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100844333</name>
        <name evidence="4" type="ORF">BRADI_1g49460v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311907.1">
        <property type="nucleotide sequence ID" value="XM_024456139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQJ5"/>
    <dbReference type="STRING" id="15368.A0A2K2DQJ5"/>
    <dbReference type="EnsemblPlants" id="PNT76556">
        <property type="protein sequence ID" value="PNT76556"/>
        <property type="gene ID" value="BRADI_1g49460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844333"/>
    <dbReference type="Gramene" id="PNT76556">
        <property type="protein sequence ID" value="PNT76556"/>
        <property type="gene ID" value="BRADI_1g49460v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DQJ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051114">
        <property type="entry name" value="Mito_RNA_Proc_CCM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934:SF13">
        <property type="entry name" value="OS06G0125300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN PET309, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="266"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="405"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="440"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT76556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="58818" checksum="12E84DDCBC4A0FF3" modified="2018-03-28" version="1">MPVRLRPAPLPLSPARGFSTTAAKTTVQLAHLAPLPSTLPPPSHCTVTPPVQPWPRRLTPRNFSRLILRLPSPHLAVLAFRHALFHSTPPLPPSLPVFAAVLSRLPGADPSLLPPVLSALRAARLPAFSDRAFLPLLRALPPLPSLRLFLSLPSFNSHPSVRSFNALLHSLVAARRLRLAAALFRAARAKLYITPDLGSCNILLKGLVGVGDLDAALKVLDEMSGFGIVPDVVTYTTVLSAYCAKGDLKGAQRLFGDIIASGRRPDVTMYTVLIDGYCRSGKIQDAARIMDEMETAGVKPNEVTYSVVIEACCKEGKSAEACNLMHEMLGAGYTPDTPLGAKVVDVLCQDGKAEEANQMWRWMVKKNVPPDNTITSTLIYWLCKSGMVQEARKLFDELERGFKPSLLTYNSLISGLCDNGELQEAGQVWDDMVERRYEPNAITYEALIKGFCKIGKPDEGAAVFTEMVTKGCTPSKSLYQVLVDSLSEPIHDDIACKILETAASSGQDFLDGNSWEIFVRKVLSTCNTWNKHLNLVLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DS61</accession>
    <name>A0A2K2DS61_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g57942v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DS61"/>
    <dbReference type="EnsemblPlants" id="PNT77115">
        <property type="protein sequence ID" value="PNT77115"/>
        <property type="gene ID" value="BRADI_1g57942v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77115">
        <property type="protein sequence ID" value="PNT77115"/>
        <property type="gene ID" value="BRADI_1g57942v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DS61"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT77115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="94" mass="10661" checksum="05D1E8402EA0BFE3" modified="2018-03-28" version="1">MARSLYLCSLRRRPEGHLGIRGELAALPSAGGLSGRAIRKKTWTFCKAELKGGDRPAQPPQWTRGLAGGEWTRCRSGRTWRRRRVGRQCENRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DTW1</accession>
    <name>A0A2K2DTW1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g67883v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DTW1"/>
    <dbReference type="EnsemblPlants" id="PNT77717">
        <property type="protein sequence ID" value="PNT77717"/>
        <property type="gene ID" value="BRADI_1g67883v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77717">
        <property type="protein sequence ID" value="PNT77717"/>
        <property type="gene ID" value="BRADI_1g67883v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DTW1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT77717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="71" mass="8135" checksum="B92C72C17E05EB8C" modified="2018-03-28" version="1">MSRKGWMRWKLKIYDMEVHSDRSIQVVESSVSPTMPAAEDHNGLKCISETCIRVLAPGNSFSDDRSALRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DUQ1</accession>
    <name>A0A2K2DUQ1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g71975v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DUQ1"/>
    <dbReference type="EnsemblPlants" id="PNT78009">
        <property type="protein sequence ID" value="PNT78009"/>
        <property type="gene ID" value="BRADI_1g71975v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78009">
        <property type="protein sequence ID" value="PNT78009"/>
        <property type="gene ID" value="BRADI_1g71975v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DUQ1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT78009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="219" mass="21616" checksum="DE21A81340F7B26A" modified="2018-03-28" version="1">MAAATAPATATKLAAEVYTAELAGVSLGAPAGVAETAEGVTAEGAPVGASVGGAGGDASGDDGAAAVGGVATGAGAVAGDLAGGAGTDGRGVDGGARGCVAGGASRCGRDRGGSHRGRSVGRGRWGGERRRRRRFGGRGGDRRGGGGWGGDRRRGGGRGPRRGRRERSHLGRRDGCRSWGLGGGGDGEEGHDCGDNGEASHLSCCDWRKNLLCLRIRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-06-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C3SA73</accession>
    <name>C3SA73_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">rRNA N-glycosylase</fullName>
            <ecNumber evidence="1">3.2.2.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC104582079</name>
        <name evidence="4" type="ORF">BRADI_1g28910v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2009" name="Plant Mol. Biol." volume="70" first="47" last="61">
            <title>Structural characterization of Brachypodium genome and its syntenic relationship with rice and wheat.</title>
            <authorList>
                <person name="Huo N."/>
                <person name="Vogel J.P."/>
                <person name="Lazo G.R."/>
                <person name="You F.M."/>
                <person name="Ma Y."/>
                <person name="McMahon S."/>
                <person name="Dvorak J."/>
                <person name="Anderson O.D."/>
                <person name="Luo M.C."/>
                <person name="Gu Y.Q."/>
            </authorList>
            <dbReference type="PubMed" id="19184460"/>
            <dbReference type="DOI" id="10.1007/s11103-009-9456-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.</text>
            <dbReference type="EC" id="3.2.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribosome-inactivating protein family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.22" evidence="1"/>
    <dbReference type="EMBL" id="EU730896">
        <property type="protein sequence ID" value="ACF22707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229689.1">
        <property type="nucleotide sequence ID" value="XM_010231387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C3SA73"/>
    <dbReference type="STRING" id="15368.C3SA73"/>
    <dbReference type="EnsemblPlants" id="KQK16468">
        <property type="protein sequence ID" value="KQK16468"/>
        <property type="gene ID" value="BRADI_1g28910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104582079"/>
    <dbReference type="Gramene" id="KQK16468">
        <property type="protein sequence ID" value="KQK16468"/>
        <property type="gene ID" value="BRADI_1g28910v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104582079"/>
    <dbReference type="eggNOG" id="ENOG502T91N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1385897_0_0_1"/>
    <dbReference type="OrthoDB" id="675370at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030598">
        <property type="term" value="F:rRNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.420.10">
        <property type="entry name" value="Ricin (A subunit), domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036041">
        <property type="entry name" value="Ribosome-inact_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017989">
        <property type="entry name" value="Ribosome_inactivat_1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001574">
        <property type="entry name" value="Ribosome_inactivat_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016138">
        <property type="entry name" value="Ribosome_inactivat_prot_sub1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33453">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33453:SF40">
        <property type="entry name" value="RRNA N-GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00161">
        <property type="entry name" value="RIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00396">
        <property type="entry name" value="SHIGARICIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56371">
        <property type="entry name" value="Ribosome inactivating proteins (RIP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="1" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0800">Toxin</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014085956" description="rRNA N-glycosylase" evidence="2">
        <location>
            <begin position="21"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ACF22707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK16468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="197" mass="21218" checksum="AB6D28A30116483B" modified="2009-06-16" version="1">MVKLVVFILVISWFVGSAVSQPPPHATPQVVTIQYDLVHGSYTSFIDDLRTKLANHPSPGDINGHPLLTPRRYPNSPARWMHISISAGGAATTTLVLRDDTLYVVGFTGEGGSWYEFGFQGEAHQIQGSIFLECGNTYKDLVGGKSGAEVRMNLQGLDLGKNMAVAAVTTLSTYVQPLPPNKPIDATKLALAARRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPL4</accession>
    <name>I1GPL4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100830258</name>
        <name evidence="4" type="ORF">BRADI_1g12540v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559584.2">
        <property type="nucleotide sequence ID" value="XM_003559536.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPL4"/>
    <dbReference type="FunCoup" id="I1GPL4">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GPL4"/>
    <dbReference type="EnsemblPlants" id="KQK13793">
        <property type="protein sequence ID" value="KQK13793"/>
        <property type="gene ID" value="BRADI_1g12540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830258"/>
    <dbReference type="Gramene" id="KQK13793">
        <property type="protein sequence ID" value="KQK13793"/>
        <property type="gene ID" value="BRADI_1g12540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830258"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0548">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_44_6_1"/>
    <dbReference type="OMA" id="ANCNITM"/>
    <dbReference type="OrthoDB" id="637363at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051616">
        <property type="entry name" value="Cul2-RING_E3_ligase_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46224">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46224:SF19">
        <property type="entry name" value="OS03G0680200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="121"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="189"/>
            <end position="218"/>
        </location>
    </feature>
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        <location>
            <begin position="260"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="442"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="337"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="342"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK13793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="502" mass="53725" checksum="4097E5FD2B89EFD0" modified="2012-06-13" version="1">MARGRRRGGGRRGRGSGPSPGSSADIAASVGLMAAQLGMCGLSSGHAEAMKRVIASSDVDRDHAMRMVEDMERNESDSTGSLEMEDFGPLHEAVSTGRMETCKYLVEQLGFDINADAKDDSGMTPLACAVLHGKAIAVRYLLDRGADLDKQDNKGFTPLHYGTKEGKDGLVRLLLSKGASVDVSSCEGTPLHIAASYGKCGIMEILLQHNADPNRVSADLGTPMAVVLCVAFTPGQMSESVALKCLKLLVKGGADLNCRNPDTPLVIATSRNLTECVEYLLEVGADANIPMNHGGMTPIEIAADSGRRKLVEILFPFTSPIQSVSNWSVEGIMVHAKSSNPKNKDKQSDKEKATKKLDTGPSKSSSKGGDKDKKAELKLLGAKAVEGKDYAGASKFYNEAIKVDPADAALYSNRSLCLLKSGEAHAALLDANACIRLRPDWPKGYYRKGAAFMVLKEYKEACDAFMAGVKLDPASVEMHEAFWDAVEAMKEHCDEQSDDSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTG8</accession>
    <name>I1GTG8_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC100842212</name>
        <name evidence="2" type="ORF">BRADI_1g24740v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562916.1">
        <property type="nucleotide sequence ID" value="XM_003562868.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GTG8"/>
    <dbReference type="STRING" id="15368.I1GTG8"/>
    <dbReference type="EnsemblPlants" id="KQK15761">
        <property type="protein sequence ID" value="KQK15761"/>
        <property type="gene ID" value="BRADI_1g24740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842212"/>
    <dbReference type="Gramene" id="KQK15761">
        <property type="protein sequence ID" value="KQK15761"/>
        <property type="gene ID" value="BRADI_1g24740v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842212"/>
    <dbReference type="eggNOG" id="ENOG502S8E0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135298_1_0_1"/>
    <dbReference type="OMA" id="KGVHMKR"/>
    <dbReference type="OrthoDB" id="1930685at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36385">
        <property type="entry name" value="OS07G0562900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36385:SF1">
        <property type="entry name" value="OS07G0562900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="110"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK15761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="110" mass="11840" checksum="A67A287FCB7B9D94" modified="2012-06-13" version="1">MAKSRNKNKAKKGGDAAPMDTSEGAPATSTATEIPQPMDTSEGKQPSSASASLSSISRKIKKGVQIKRTKNMRKMKAVARAISKTEKSEEKVLKAKSKKTRIQSAKSLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1GV95</accession>
    <name>I1GV95_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100846181</name>
        <name evidence="7" type="ORF">BRADI_1g30000v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563265.1">
        <property type="nucleotide sequence ID" value="XM_003563217.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GV95"/>
    <dbReference type="STRING" id="15368.I1GV95"/>
    <dbReference type="EnsemblPlants" id="KQK16691">
        <property type="protein sequence ID" value="KQK16691"/>
        <property type="gene ID" value="BRADI_1g30000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846181"/>
    <dbReference type="Gramene" id="KQK16691">
        <property type="protein sequence ID" value="KQK16691"/>
        <property type="gene ID" value="BRADI_1g30000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846181"/>
    <dbReference type="eggNOG" id="ENOG502QQHH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035660_1_1_1"/>
    <dbReference type="OMA" id="WIYNEED"/>
    <dbReference type="OrthoDB" id="752464at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04873">
        <property type="entry name" value="ACT_UUR-ACR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054502">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051358">
        <property type="entry name" value="TF_AMS/ICE1/BHLH6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945">
        <property type="entry name" value="TRANSCRIPTION FACTOR SCREAM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22754">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="153"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK16691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="330" mass="36374" checksum="859879749AE91963" modified="2012-06-13" version="1">MELDEQAFLEELFSLRRDAAWECNAMPMGDFFSPGCGAAAMDCFQERHQPTVSVLPTFTASYDHPHPPPPPQQQPGFDYLSEVYGSSGVVPFSSGGGEYGGDMGFLDVMEPKVAAPMAAEVVGACKVEPGLADGGAFSAGPPAPASRKKRVEGMPSKNLMAERRRRKRLNDRLSMLRSVVPKISKMDRTSILGDTIDYMKELLERIRQLQEEMEQEGAPETAPAPALLSVFRREQNPNEMLARNTPKFEVERKEKDDTRVEIYCAAKPGLLLSTVSTLDTLGLDIQQCVVSCFNDFAMHASCSEMQREMITTEVIKQELYKNAGYGGGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1GY88</accession>
    <name>I1GY88_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100833775</name>
        <name evidence="5" type="ORF">BRADI_1g38790v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563803.1">
        <property type="nucleotide sequence ID" value="XM_003563755.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GY88"/>
    <dbReference type="STRING" id="15368.I1GY88"/>
    <dbReference type="EnsemblPlants" id="KQK18111">
        <property type="protein sequence ID" value="KQK18111"/>
        <property type="gene ID" value="BRADI_1g38790v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833775"/>
    <dbReference type="Gramene" id="KQK18111">
        <property type="protein sequence ID" value="KQK18111"/>
        <property type="gene ID" value="BRADI_1g38790v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833775"/>
    <dbReference type="eggNOG" id="ENOG502QQGA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021337_0_0_1"/>
    <dbReference type="OMA" id="DHKGVEN"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15488">
        <property type="entry name" value="Tm-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12030">
        <property type="entry name" value="Uncharacterised protein family UPF0261, NC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12020">
        <property type="entry name" value="Uncharacterised protein family UPF0261, NN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009215">
        <property type="entry name" value="TIM-br_IGPS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051353">
        <property type="entry name" value="Tobamovirus_resist_UPF0261"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056778">
        <property type="entry name" value="UPF0261_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044122">
        <property type="entry name" value="UPF0261_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002674">
        <property type="entry name" value="PRK02399.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31862">
        <property type="entry name" value="UPF0261 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G10120)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31862:SF1">
        <property type="entry name" value="UPF0261 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G10120)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09370">
        <property type="entry name" value="PEP_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06792">
        <property type="entry name" value="UPF0261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23189">
        <property type="entry name" value="UPF0261_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0261" evidence="2">
        <location>
            <begin position="5"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="UPF0261" evidence="4">
        <location>
            <begin position="203"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="TIM-barrel" evidence="3">
        <location>
            <begin position="479"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK18111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="747" mass="79828" checksum="61F0A8D64A055CED" modified="2012-06-13" version="1">MTEMEVLCIGTADTKLEELLFLAARLRSTLPASASIPKVQVSIVDVSTTKTIPTQDTKDISVIARDTVLSCYPDANQQDLPDDRGEAIAYMSKALQCFLKNKYKGGTLVGAVGLGGSGGTALIAPALRSLPLRVPKLIISTVASGHTAPYVGTSDLVLFPSVVDICGINSVSRVILSNAASAVAGMVHGILLASSKSDETDTKLTVGITMFGVTTPCANAVKDRLNKEGYETLVFHATGVGGKAMEELVRSGFIQGVLDITTTEVVDYIVGGIMACDKTRFDAAIDNNIPLVVSVGALDMVNFGAHDTIPPAFSDRKIHVHNEQISLMRTTVEENKKCAQFISEKLNKSSSRVTVCLPQKGVSAIDAPGMPFYDPEATSALLDELNTRIVKTDNRQLKLLPYHINDPEFANALVDAFLSMDIKASIALTQKSNLILPKQDANKQESSSEQRASDNSIIWRPPMDYPDAKPETLQKTNSIIYKLKQQICEGTPVIGAGAGTGISAKFEEAGGVDLIVLYNSGRFRMAGRGSLAGLLPFADANATVLEMANEVLPVVKEVPVLAGVCATDPFRRMDYFLKQLEAIGFCGVQNFPTVGLYDGNFRQNLEETGMGYSMEVDMISRARNMGFLTTPSAFNPAEGAAMAKAGAHIIVPHMGLTTAGSIGAKTAATLDESVLRVQAIADAAIGVNPDIIILCHGGPISGPREAEFILKNTKRVHGFYGASSMERLPVEKAITNTMRKYKCISLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H0N3</accession>
    <name>I1H0N3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100845272</name>
        <name evidence="8" type="ORF">BRADI_1g48220v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564259.1">
        <property type="nucleotide sequence ID" value="XM_003564211.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H0N3"/>
    <dbReference type="FunCoup" id="I1H0N3">
        <property type="interactions" value="2828"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H0N3"/>
    <dbReference type="EnsemblPlants" id="KQK19424">
        <property type="protein sequence ID" value="KQK19424"/>
        <property type="gene ID" value="BRADI_1g48220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845272"/>
    <dbReference type="Gramene" id="KQK19424">
        <property type="protein sequence ID" value="KQK19424"/>
        <property type="gene ID" value="BRADI_1g48220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845272"/>
    <dbReference type="eggNOG" id="KOG0178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_3_1"/>
    <dbReference type="OMA" id="YVLNDNM"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03752">
        <property type="entry name" value="proteasome_alpha_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000031">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK19424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="250" mass="26951" checksum="8D327C6408D598BF" modified="2012-06-13" version="1">MSRRYDSRTTIFSPEGRLYQVEYAMEAIGNAGSALGILAADGVVLVGEKKVTSKLLQSSRSAEKMYKIDSHLACAVAGIMSDANILINTARLHAQRYALSYQEAIPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFGGWDKKHGFQLYMSDPSGNYGGWKAAAVGANSQAAQSMLKQDYRDGMTREEAVALALKVLSKTMDSTSLTAEKLELAEVFLQPGTGEVQYQVCSPDAMGKLLAKAGLTQPAPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1H1T1</accession>
    <name>I1H1T1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">YEATS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100828557</name>
        <name evidence="7" type="ORF">BRADI_1g51500v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
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                <person name="Vogel J.P."/>
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                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
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    </feature>
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        <source>
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    </evidence>
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</entry>
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    </gene>
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the LEA type SMP family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.I1H5D6"/>
    <dbReference type="EnsemblPlants" id="KQK21651">
        <property type="protein sequence ID" value="KQK21651"/>
        <property type="gene ID" value="BRADI_1g62180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825069"/>
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        <property type="protein sequence ID" value="KQK21651"/>
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    </dbReference>
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        <property type="entry name" value="LEA_SMP"/>
    </dbReference>
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        <property type="entry name" value="LEA_SMP_dom"/>
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        <property type="entry name" value="OS12G0626500 PROTEIN"/>
        <property type="match status" value="1"/>
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            <end position="222"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK21651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="240" mass="24164" checksum="ED522FE58C99FC99" modified="2012-06-13" version="1">MSGFDEHHHGGGQAQPLRPGDVYPPTAANPEARRQRDVFLAASARHDQQQQQPPRPDDGGLRVTETEDNHTGRRIVTATAGGQVMAQFTVPVPGAGDDSAVTIGEALRAAADTSAGDEPVDLADAAAVQAAEARATGLGHNVPGGVAAAAQKAAQENLEREGGREKKVPLKDVVGGDLVGRGPALAADKVATREDAAKVAAAAERNAASKSGGAGAAGGQGKGVADAVAAAAEMNQARMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1H603</accession>
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        <recommendedName>
            <fullName evidence="4">HIT domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="5" type="ORF">BRADI_1g63860v3</name>
    </gene>
    <organism evidence="5">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003557973.4"/>
    </dbReference>
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    <dbReference type="FunCoup" id="I1H603">
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    </dbReference>
    <dbReference type="STRING" id="15368.I1H603"/>
    <dbReference type="EnsemblPlants" id="KQK21906">
        <property type="protein sequence ID" value="KQK21906"/>
        <property type="gene ID" value="BRADI_1g63860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842067"/>
    <dbReference type="Gramene" id="KQK21906">
        <property type="protein sequence ID" value="KQK21906"/>
        <property type="gene ID" value="BRADI_1g63860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842067"/>
    <dbReference type="eggNOG" id="KOG3275">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="OMA" id="YRVVMNC"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
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        <property type="term" value="F:adenylylsulfatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009150">
        <property type="term" value="P:purine ribonucleotide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:sulfur compound metabolic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Histidine triad nucleotide-binding protein 1"/>
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        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="19"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="113"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK21906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="129" mass="14203" checksum="509B8C81D32B3A73" modified="2012-06-13" version="1">MASEKDAALAAVPNDNPTIFDKIIKKEIPSTVVYEDEKVLAFRDINPQAPIHIIIIPKVRDGLTGLSKAEERHVEILGSLLYVAKVIAKQEGLEDGYRIVINDGPSGCQSVYHIHVHLLGGRQMNWPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6T9</accession>
    <name>I1H6T9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100825380</name>
        <name evidence="13" type="ORF">BRADI_1g66220v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561764.1">
        <property type="nucleotide sequence ID" value="XM_003561716.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6T9"/>
    <dbReference type="STRING" id="15368.I1H6T9"/>
    <dbReference type="EnsemblPlants" id="KQK22277">
        <property type="protein sequence ID" value="KQK22277"/>
        <property type="gene ID" value="BRADI_1g66220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825380"/>
    <dbReference type="Gramene" id="KQK22277">
        <property type="protein sequence ID" value="KQK22277"/>
        <property type="gene ID" value="BRADI_1g66220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825380"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_49_0_1"/>
    <dbReference type="OMA" id="VIKFRRH"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000217">
        <property type="entry name" value="Ankyrin repeat-containing protein ITN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000245">
        <property type="entry name" value="Ankyrin repeat-containing protein ITN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF48">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN ITN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="555"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="595"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="621"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="326"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="12">
        <location>
            <begin position="508"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK22277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="685" mass="74010" checksum="C445FF63BBA7C59C" modified="2012-06-13" version="1">MAVDPARGEGDLEIGLASPESDGGVSPVVGPGRQALDQSSPRAVRRPGLVMSNSGKRLDQSPLSSPSRPVLLMSHSSNRLEYQAPGRPALVMSRSSNRLDQSPASSPTPTKGPALVMSGSSKRLEQSLASPSPSPKAGAAAPPVLVLSNSGKRMDQAGRKKYVKQVTGRQNDTELHLAAQRGDLDAVRQIIAEIDAQMTGTGEEFDSEVAEIRAAVVNETNEVEETALLIAAEKGFLDIVIELLKHSDKESLARKNKSGFDALHVAAKEGRRDVVKVLLDHDPSLGKTFGQSNVTPLITAAIRGHIEVVNLLLERVSGLVELSKANGKNALHFAARQGHVEIVQSLLDSDPQLARRTDKKGQTALHMAVKGTSAGVVRALVNADPAIVMLPDRNGNLALHVATRKKRSEIVNVLLLLPDMNVNALTRDRKTAFDIAEGLPLSEESAEIKDCLSRAGAVRANDLNQPRDELRKTVTEIKKDVHTQLEQARKTNKNVHGIAKELRKLHREGINNATNSVTVVAVLFATVAFAAIFTVPGGNDDHGVAIAVHAVSFKVFFLFNAVALFTSLAVVVVQITLVRGETKAERRVVEVINKLMWLASVCTTVAFISSSYIVVGRHFRWAALLVTLIGGVIMTGVLGTMTYYVVKSKRTRLIRKKVKSRRSGSHSWQHNSEFSDSEIDRIYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1H8A1</accession>
    <name>I1H8A1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100842792</name>
        <name evidence="5" type="ORF">BRADI_1g70460v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558525.1">
        <property type="nucleotide sequence ID" value="XM_003558477.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H8A1"/>
    <dbReference type="EnsemblPlants" id="KQK22981">
        <property type="protein sequence ID" value="KQK22981"/>
        <property type="gene ID" value="BRADI_1g70460v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK22982">
        <property type="protein sequence ID" value="KQK22982"/>
        <property type="gene ID" value="BRADI_1g70460v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK22983">
        <property type="protein sequence ID" value="KQK22983"/>
        <property type="gene ID" value="BRADI_1g70460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842792"/>
    <dbReference type="Gramene" id="KQK22981">
        <property type="protein sequence ID" value="KQK22981"/>
        <property type="gene ID" value="BRADI_1g70460v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22982">
        <property type="protein sequence ID" value="KQK22982"/>
        <property type="gene ID" value="BRADI_1g70460v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22983">
        <property type="protein sequence ID" value="KQK22983"/>
        <property type="gene ID" value="BRADI_1g70460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842792"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038211_1_1_1"/>
    <dbReference type="OMA" id="MESPNDH"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000348">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46225">
        <property type="entry name" value="C3H4 TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46225:SF19">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="239"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="338"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK22982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="407" mass="44671" checksum="A33D5C3E1133D412" modified="2012-06-13" version="1">MQSGMEHTTGVSGRDHRIDIPRDTGPSSVSVSHSVGRENQEELNPVDRPSTRALTSALQPPAAVGPPHAGNTSGARRGDNYGRRHRSPLNSGLWISIEVIVNVSQIVAAIVVLSLSRKEHPQAPLFEWVIGYTVGCFATLPHLYWRYIHRNIVNGENEQAHSLQGSSQSQNNSTEPTHAASASERRRTAARNAVLANPRINALFDHFKMALDCFFAVWFVVGNVWIFGGRSSAADAPNLYRLCIVFLTFSCIGYAMPFILCAMICCCLPCIISVMGFREDTNNTRGATSESINSLPTYKFKTKKRRHGSGNDAEGQDGGILAAGTDKERSLSAEDAVCCICLAKYAHNDELRELACTHCFHKECVDKWLKINALCPLCKSEIASSSGTSDTRRTDHTAIQVQEIEMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HA56</accession>
    <name>I1HA56_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4-coumarate--CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100831811</name>
        <name evidence="12" type="ORF">BRADI_1g76430v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(E)-4-coumarate + ATP + CoA = (E)-4-coumaroyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19641"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19642"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(E)-4-coumarate + ATP + H(+) = (E)-4-coumaroyl-AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:72419"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:72420"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(E)-4-coumaroyl-AMP + CoA = (E)-4-coumaroyl-CoA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:72423"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:72424"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.12" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229258.1">
        <property type="nucleotide sequence ID" value="XM_010230956.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HA56"/>
    <dbReference type="STRING" id="15368.I1HA56"/>
    <dbReference type="EnsemblPlants" id="KQK23834">
        <property type="protein sequence ID" value="KQK23834"/>
        <property type="gene ID" value="BRADI_1g76430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831811"/>
    <dbReference type="Gramene" id="KQK23834">
        <property type="protein sequence ID" value="KQK23834"/>
        <property type="gene ID" value="BRADI_1g76430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831811"/>
    <dbReference type="eggNOG" id="KOG1176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_1"/>
    <dbReference type="OMA" id="IRLMYTS"/>
    <dbReference type="OrthoDB" id="10253115at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016207">
        <property type="term" value="F:4-coumarate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106290">
        <property type="term" value="F:trans-cinnamate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12118">
        <property type="entry name" value="ttLC_FACS_AEE21_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006020">
        <property type="entry name" value="PRK08162.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859:SF2">
        <property type="entry name" value="BUTYRATE--COA LIGASE AAE11, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="11">
        <location>
            <begin position="458"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="548"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK23834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="596" mass="64635" checksum="DF52679B8061FB20" modified="2012-06-13" version="1">MEQLPKRAANYVPLSPVGFLPRANAVYGDRTSLVYGRRVRFTWSQTHDRCRRLASSLLALGVRKNDVVSVLAPNVPAMYEMHFAVPMAGAVLNTVNTRLDAKAVAAILRHSEAKLFFVDYEYVRLASDALQLLVSDGASQVPLVAVIDDLDRPTGVRLGELEYEGLVSRGDPTVQLPMLADEWDAVTLNYTSGTTSAPKGVVYSHRGAYLSTTSLLMSWEMGAEPVYLWTLPMFHCNGWTFTWGVAARGGVNVCIRDARAADVYGAIARHGVTHMCCAPVVFNILLEGAAATSAPNQRQLAAPVHVLTGGAPPPAALLERVERIGFHVTHAYGLTEATGPALACEWRAQWDHLPVSDRARLKARQGVSVLSLADADVINEDTMSSVQRDGKALGEIVLRGSSVMKGYLKNPEANEAAFKGGWFMTGDVGVVHPDGYIEIKDRSKDVIISGGENICSKEVEEALFAHPAVADAAVVAMPHPHWGETPCAFVVPRKKEDGDQNVQVCEEEVVDFCRKRMARFMVPRKVVLCEALPRNALGKVEKVKLRDAARKLQPAPAQKTTTSNSKGSTKAPAGKSGGGRRDEQNPVAHVMAMSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HD19</accession>
    <name>I1HD19_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BGGP Beta-1-3-galactosyl-O-glycosyl-glycoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100841315</name>
        <name evidence="8" type="ORF">BRADI_2g06240v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003565420.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HD19"/>
    <dbReference type="EnsemblPlants" id="KQK03198">
        <property type="protein sequence ID" value="KQK03198"/>
        <property type="gene ID" value="BRADI_2g06240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841315"/>
    <dbReference type="Gramene" id="KQK03198">
        <property type="protein sequence ID" value="KQK03198"/>
        <property type="gene ID" value="BRADI_2g06240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841315"/>
    <dbReference type="eggNOG" id="KOG0799">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="GLCAT14A/B/C"/>
    </dbReference>
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        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45719">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45719:SF7">
        <property type="entry name" value="OS01G0201100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK03198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="52965" checksum="527F6370A731E8F6" modified="2012-06-13" version="1">MGASGRGAAEQQPTGAKPSPRIGEQHYPASSTKTSPRAGTGIGIGVGVPSMLPTALLESKWALSAAITVFLFLAAALTFTSSPAISASSFLSFLPASHPQQHDQQQQPASSSPPPAAADPGAGVPRLAYLVSGSKGDLDRLWRTLHALYHPRNLYVVHLDRESPVGERLELAARVANSTVFRRVGNVEVIRRANMVTYRGPTMVANTLHACAVLLRRSRDWDWFINLSASDYPLMTQDDILHVFSTLPRNVNFIEHTGNLGWKEGQRGRPVIVDPGLYSSQKQDIFYTSPHRELPTAFKLYTGSAWVALTRDFAEYVVWGWDNLPRTLLMYYSNFVSSPEGYFQTVACNAPRFVPTVANHDLHHIQWDVPPRQHPHPLGLADMDRMLRSDAPFARKFGRDDPVLDAIDRQLLRGRGGSNVNGTASSSSSGMFVPGGWCGENGDCVNSGGDQDWVLRPGPGAERLKRLMDRIVRSEAFANSQCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1HGC3</accession>
    <name>I1HGC3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Negatively light-regulated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100843260</name>
        <name evidence="4" type="ORF">BRADI_2g16310v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567883.1">
        <property type="nucleotide sequence ID" value="XM_003567835.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HGC3"/>
    <dbReference type="EnsemblPlants" id="KQK04840">
        <property type="protein sequence ID" value="KQK04840"/>
        <property type="gene ID" value="BRADI_2g16310v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT70694">
        <property type="protein sequence ID" value="PNT70694"/>
        <property type="gene ID" value="BRADI_2g16310v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843260"/>
    <dbReference type="Gramene" id="KQK04840">
        <property type="protein sequence ID" value="KQK04840"/>
        <property type="gene ID" value="BRADI_2g16310v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70694">
        <property type="protein sequence ID" value="PNT70694"/>
        <property type="gene ID" value="BRADI_2g16310v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843260"/>
    <dbReference type="eggNOG" id="ENOG502S1VS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094393_1_0_1"/>
    <dbReference type="OMA" id="CLFDPFP"/>
    <dbReference type="OrthoDB" id="1902088at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF46">
        <property type="entry name" value="OS05G0566600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK04840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15299" checksum="918010F67322C996" modified="2012-06-13" version="1">MSGKPSDDMAGQVRPEGDVSDVKVETANQNKGTDMPSPQEEEAAIKKKYGGVLPKKSPLISKDHERAYFDSADWALGKQGGHPKPKGPLEALRPKLQPTQQQARSRRFLHASADNEEGGNSPTEATSQNQDSTEGKDESKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HGG5</accession>
    <name>I1HGG5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Drought induced 19 protein type zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100824984</name>
        <name evidence="5" type="ORF">BRADI_2g16670v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Di19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231032.1">
        <property type="nucleotide sequence ID" value="XM_010232730.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HGG5"/>
    <dbReference type="FunCoup" id="I1HGG5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HGG5"/>
    <dbReference type="EnsemblPlants" id="KQK04898">
        <property type="protein sequence ID" value="KQK04898"/>
        <property type="gene ID" value="BRADI_2g16670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824984"/>
    <dbReference type="Gramene" id="KQK04898">
        <property type="protein sequence ID" value="KQK04898"/>
        <property type="gene ID" value="BRADI_2g16670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824984"/>
    <dbReference type="eggNOG" id="ENOG502QVUG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072240_0_0_1"/>
    <dbReference type="OMA" id="FEVEDDM"/>
    <dbReference type="OrthoDB" id="7873042at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033347">
        <property type="entry name" value="Di19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027935">
        <property type="entry name" value="Di19_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31875">
        <property type="entry name" value="PROTEIN DEHYDRATION-INDUCED 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31875:SF6">
        <property type="entry name" value="PROTEIN DEHYDRATION-INDUCED 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14571">
        <property type="entry name" value="Di19_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Di19 zinc-binding" evidence="3">
        <location>
            <begin position="45"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Di19 C-terminal" evidence="4">
        <location>
            <begin position="117"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK04898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25673" checksum="F7C96EA65DE313D5" modified="2012-06-13" version="1">MDSDHWISRLAAAKRFYAAQLGHGDRGMEELDMDNEEEEGGRAAEFACPYCYEDHDVASLCAHLEEEHPFEPHAAPCPICSHKITRDMLNHITMQHGYLFKNRRRLRRFTVPGSQSLSLLSRDLREAHLQLLLGGGGHRSSNNNATNISADPLLSSFGLSIPTPDAEETSILPISAPSDASVLKETHVQPWQSSIDSSLTSEEREQKREQASGRATFVQGLLLSTLFRDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HIL3</accession>
    <accession>A0A0Q3G3G9</accession>
    <name>I1HIL3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100832139</name>
        <name evidence="4" type="ORF">BRADI_2g22850v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05840.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568275.1">
        <property type="nucleotide sequence ID" value="XM_003568227.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HIL3"/>
    <dbReference type="FunCoup" id="I1HIL3">
        <property type="interactions" value="565"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HIL3"/>
    <dbReference type="EnsemblPlants" id="KQK05838">
        <property type="protein sequence ID" value="KQK05838"/>
        <property type="gene ID" value="BRADI_2g22850v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK05840">
        <property type="protein sequence ID" value="KQK05840"/>
        <property type="gene ID" value="BRADI_2g22850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832139"/>
    <dbReference type="Gramene" id="KQK05838">
        <property type="protein sequence ID" value="KQK05838"/>
        <property type="gene ID" value="BRADI_2g22850v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05840">
        <property type="protein sequence ID" value="KQK05840"/>
        <property type="gene ID" value="BRADI_2g22850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832139"/>
    <dbReference type="eggNOG" id="KOG2058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008366_0_0_1"/>
    <dbReference type="OMA" id="WKHFLER"/>
    <dbReference type="OrthoDB" id="17687at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HIL3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000013">
        <property type="entry name" value="TBC1 domain family member 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000018">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="198"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="143"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="699"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="759"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="590"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="708"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="768"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK05840.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="827" mass="93428" checksum="B7153F24446D9561" modified="2012-06-13" version="1">MKPKSLPFIAFEHKRDAYGFAVRPQHLQRYKEYAGIYKEEEEERSDRWKHFLERQDVPSGHSVNVELSVDEAEAVVCVEQNVETGSSELLHEKSTQEKSESWKPIRPSLGNIEQMMGLRVDKKHSSAGRLQPKESTHLVTVEEGKVSGDSDDEFYDADKVDPSQEMQSGDSGNAEIGNTGQEDKYSLKEELECLVHGGLPMALRGELWQAFVGVEARRVNGYYDSLLVTEGELEDGRLDSSTSEGVDEKLSAFSSEKWKGQIEKDLPRTFPGHPSLDEDGRNALRRLLLAYARHNPTVGYCQAMNFFAGLLLLLMPEENAFWTLVGIIDDYFDGYFSEEMIESQVDQLVLEELVQEKFPKLANHLNYLGLEVTWATGPWFLSIFANVLPWESVLRVWDVLLFDGNRVMLFRTALALLEFYGPALVTTKDAGDAVTLLQSLAGSTFDSSQLVLTARMGYQSVNEARLQDLRNKHRPSVISSMEERAKGLGVCKDSGLASKLYNFKREPEPLVSISNSLDQMSDVTDGDVNNESGSGDMDDMYGGLTVNSEIDSLPDPKDQVIWLKVELCRLLEERRSAVLRADELETALMEMVKQDNRRQLSAKAEQLEHELSELRQTLSDKQEQEQAMFQVLMRVEQELKFAEEARISAEQDAAAQRYAANVLQEKYEESMASLAQMENRAVMAETMLEATLQYQSSQQKALSPLPSPRTSVQDDSVQEFQPRRINLLGPFSLSWRDKNKGKQNNADDCMDAKLTDTLDQWEETPNTDDAKQGETPKLDGEWTVELPKGDDELMAETPKNDSELSRVHIVTNVMNGQHEQLQEINLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJK3</accession>
    <name>I1HJK3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100823158</name>
        <name evidence="2" type="ORF">BRADI_2g25807v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568499.1">
        <property type="nucleotide sequence ID" value="XM_003568451.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014755240.1">
        <property type="nucleotide sequence ID" value="XM_014899754.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJK3"/>
    <dbReference type="STRING" id="15368.I1HJK3"/>
    <dbReference type="EnsemblPlants" id="KQK06337">
        <property type="protein sequence ID" value="KQK06337"/>
        <property type="gene ID" value="BRADI_2g25807v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823158"/>
    <dbReference type="Gramene" id="KQK06337">
        <property type="protein sequence ID" value="KQK06337"/>
        <property type="gene ID" value="BRADI_2g25807v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823158"/>
    <dbReference type="eggNOG" id="ENOG502S8K8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186390_0_0_1"/>
    <dbReference type="OMA" id="ACHERKM"/>
    <dbReference type="OrthoDB" id="5586401at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48236">
        <property type="entry name" value="COX19-LIKE CHCH FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48236:SF1">
        <property type="entry name" value="COX19-LIKE CHCH FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9216" checksum="411EF6C7DF8B3A5D" modified="2012-06-13" version="1">MGSSSSSNAQDTPAPPPPPPPAPPAPLHVMDADEDDDNVKQLNECAALYLSLQDCLSESDRNWKACQAHVQALKACEASRNRSEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I1HM53</accession>
    <name>I1HM53_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100839204</name>
        <name evidence="2" type="ORF">BRADI_2g36840v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568978.1">
        <property type="nucleotide sequence ID" value="XM_003568930.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HM53"/>
    <dbReference type="EnsemblPlants" id="KQK07658">
        <property type="protein sequence ID" value="KQK07658"/>
        <property type="gene ID" value="BRADI_2g36840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839204"/>
    <dbReference type="Gramene" id="KQK07658">
        <property type="protein sequence ID" value="KQK07658"/>
        <property type="gene ID" value="BRADI_2g36840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839204"/>
    <dbReference type="eggNOG" id="ENOG502R571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008956_6_0_1"/>
    <dbReference type="OMA" id="KELPMAN"/>
    <dbReference type="OrthoDB" id="588941at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011676">
        <property type="entry name" value="DUF1618"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074:SF49">
        <property type="entry name" value="OS07G0258000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07762">
        <property type="entry name" value="DUF1618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1618" evidence="1">
        <location>
            <begin position="222"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK07658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="440" mass="49319" checksum="E476241192CD6FE2" modified="2012-06-13" version="1">MAVSSWVPPSLPESGASGLVLLDKCCYIADLRNDTTAESTTSGGLPLKVTFRAARPPLVSHFCVYCPGLDFRSKGPPKIVATDADLVLLSVPAVDPNSSSRFDRDYFVYSTRAPWLHLLPNPHPRRLHHSSTALISRQAGASYAVAALGICSSLYDGDTLLRWEFDLHLYRSSDSKGWISKRLSVNGLERDKLIPLPRGVDRLYHETGKTITVGGDHGTVAWVDLWRGIFFCDVLKKRPRLRDVPLPAPARRNWERLLRNSDPGYLRDVTISRNKDSIKYVELEFLESIKELDATVPDSYADWVRNRSRKSQVIVRDGWKSTTWNMAIPAGSSSWRRDCVLDVKDVGLEEAGDPCLSHLMAMLSGKTIKELPVGYPILSMDDDVVYLRSETRVPRHMEKLALMFSIDVRKAELRGWAELDAKKSSNFLPAFCTSEICRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1HN68</accession>
    <name>I1HN68_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836335</name>
        <name evidence="3" type="ORF">BRADI_2g39840v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569134.1">
        <property type="nucleotide sequence ID" value="XM_003569086.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HN68"/>
    <dbReference type="STRING" id="15368.I1HN68"/>
    <dbReference type="EnsemblPlants" id="KQK08130">
        <property type="protein sequence ID" value="KQK08130"/>
        <property type="gene ID" value="BRADI_2g39840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836335"/>
    <dbReference type="Gramene" id="KQK08130">
        <property type="protein sequence ID" value="KQK08130"/>
        <property type="gene ID" value="BRADI_2g39840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836335"/>
    <dbReference type="eggNOG" id="KOG2063">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009467_0_0_1"/>
    <dbReference type="OMA" id="MFVTSEG"/>
    <dbReference type="OrthoDB" id="10258882at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HN68">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010015">
        <property type="term" value="P:root morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019452">
        <property type="entry name" value="VPS39/TGF_beta_rcpt-assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019453">
        <property type="entry name" value="VPS39/TGFA1_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF43">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10366">
        <property type="entry name" value="Vps39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10367">
        <property type="entry name" value="zf-Vps39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CNH" evidence="2">
        <location>
            <begin position="31"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="1">
        <location>
            <begin position="634"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK08130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="945" mass="104013" checksum="6EBC62161FBFEA42" modified="2012-06-13" version="1">MGSHPAPSRAALEPFATLDPAALAALPASTPLTVRSAALSAPHLLYLGTGGGKLLLFSLQTPSSPEFLRLLPIGATRPVSAIVPLPSVARVLVLAEGILLLADPLLARPVRRLGSLRHVAAVAVATATATATDPSSSSSCSLAVAVGKKLLLVDLTLHEADELEVQTREIPAAAGVEGITVLAWIGDSVFAGTKTGYSLFSTSTGQAVELFTLPESAGSPRIKPLSGGDEAMLLVDNVGVVIDRSGQPVGSSFVFNSRPDCIVEVFPYVVVAGESKVDVYRRKNGAHLQTVPIARSGSGVLTVASNNDGSGGDVVVIATAYKVFCYRKVSAVEQIKASLRIKSYAEAISLLEEFESDGEISKDMISFVHAQLGFLLFFDLHFEDAVNHFLLSETMQPTEIFPFIMRGPNRWSDMVPRKRYWGLHPPPKPLEEVIDDGLVTLQRALFLKKAGVDTDVDEDFLSNPPTRADLLELAIRNIIRYLCVSREKTLSPAEMEGVDTLLMYLYRALDLVDDMEKLASSENSCVVEELESLLDNSGHLRTLAFLYGSKGMCPQAVSIWRILARNYSTGLWKDRANLPETDSFKTPVDKKSGEEIAAIEASKILEASSDQDLVLEHLGWVADIDQELAVSILTSEARENQLSAEKVVAALDSEKVGIHQRYLQWLIEDNGCDDPHYHTSYALLLANSAMEAFHLESNSGGKNDKEIDSDIQFIFALRERLQLFLQASDLYDPEEVLDVIAESELWLEKAILYRKMGQENIVLQILALKLEDSEAAEQYCAEIGRDDAYIQLLDLYLDPKNGKEPMFTAAVRLLHKHGKSLDPLQVLERLSPEMPLQLASDTILRMLRARVHHHRQGQIVHNLSRATNLDARLTRLEERSRHVQLTDESICDSCRARLGTKLFVMYPDDSVVCYRCYRNQGDSVSGRGRNFRKDAIFKQSWLVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HP01</accession>
    <name>I1HP01_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Holocarboxylase synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100830936</name>
        <name evidence="2" type="ORF">BRADI_2g42270v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569287.1">
        <property type="nucleotide sequence ID" value="XM_003569239.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HP01"/>
    <dbReference type="FunCoup" id="I1HP01">
        <property type="interactions" value="1769"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HP01"/>
    <dbReference type="EnsemblPlants" id="KQK08513">
        <property type="protein sequence ID" value="KQK08513"/>
        <property type="gene ID" value="BRADI_2g42270v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830936"/>
    <dbReference type="Gramene" id="KQK08513">
        <property type="protein sequence ID" value="KQK08513"/>
        <property type="gene ID" value="BRADI_2g42270v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830936"/>
    <dbReference type="eggNOG" id="ENOG502QQY0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083167_2_0_1"/>
    <dbReference type="OMA" id="HQIRDVN"/>
    <dbReference type="OrthoDB" id="755598at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016549">
        <property type="entry name" value="UCP009193"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33675:SF1">
        <property type="entry name" value="HOLOCARBOXYLASE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33675">
        <property type="entry name" value="NUCLEAR RECEPTOR FAMILY 2 GROUP C PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009193">
        <property type="entry name" value="UCP009193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK08513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="21606" checksum="CD6C36A5E1DAC4B2" modified="2012-06-13" version="1">MARKRKSDAAPRLDEADRTLYSTFCGAANSLSQLYSQAMSQQKLSFQAGERHALEKLHQWISRKHAEESRLTVTDIMSHIQHEIDSGGTDALVSPRGNQHPASANPFTNIQPSAGLYPQATAGLAPRPSLSDQPKNMIFSNALSSPVCRTLQNYHLTQGAGSGGRNNEANSVGQHREANSASSNDTSMDMVSDSAGNGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQ38</accession>
    <name>I1HQ38_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100844674</name>
        <name evidence="10" type="ORF">BRADI_2g45750v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the type IV zinc-finger family. Class B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569499.1">
        <property type="nucleotide sequence ID" value="XM_003569451.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQ38"/>
    <dbReference type="EnsemblPlants" id="KQK09052">
        <property type="protein sequence ID" value="KQK09052"/>
        <property type="gene ID" value="BRADI_2g45750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844674"/>
    <dbReference type="Gramene" id="KQK09052">
        <property type="protein sequence ID" value="KQK09052"/>
        <property type="gene ID" value="BRADI_2g45750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844674"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062129_1_0_1"/>
    <dbReference type="OMA" id="DHYYKHC"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46813">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46813:SF15">
        <property type="entry name" value="GATA-TYPE ZINC FINGER TRANSCRIPTION FACTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="9">
        <location>
            <begin position="115"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK09052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="249" mass="25952" checksum="A038A6BE3D4AB436" modified="2012-06-13" version="1">MLHEAAPCTCGLLYGSCGGGCSMLFATAAPGDYHHHYYSNSKQCGDDSGFNGSSYGGGSVDCTLSLGTPSTRRAAEAAAGLPWETAAVSSCNGNARQETIATAPRTDHQSANNNASAARRCANCDTTSTPLWRNGPRGPKSLCNACGIRYKKEERRAAAAAVAPTAAGLASDSGMEYAYGYARQQQQHQQQQWGCYGPAVAKAAASYGMFGDAAVAEVDGPCLPWGLGVMPSSPAFGSVREMPSLFQYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HTS8</accession>
    <name>I1HTS8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AUGMIN subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100827455</name>
        <name evidence="2" type="ORF">BRADI_2g56240v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564707.1">
        <property type="nucleotide sequence ID" value="XM_003564659.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HTS8"/>
    <dbReference type="FunCoup" id="I1HTS8">
        <property type="interactions" value="1732"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HTS8"/>
    <dbReference type="EnsemblPlants" id="KQK10794">
        <property type="protein sequence ID" value="KQK10794"/>
        <property type="gene ID" value="BRADI_2g56240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827455"/>
    <dbReference type="Gramene" id="KQK10794">
        <property type="protein sequence ID" value="KQK10794"/>
        <property type="gene ID" value="BRADI_2g56240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827455"/>
    <dbReference type="eggNOG" id="ENOG502QR4M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073448_0_0_1"/>
    <dbReference type="OMA" id="SMYADNP"/>
    <dbReference type="OrthoDB" id="1920495at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009524">
        <property type="term" value="C:phragmoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031023">
        <property type="term" value="P:microtubule organizing center organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029711">
        <property type="entry name" value="Haus7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010604">
        <property type="entry name" value="Plant_AUG7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14352">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14352:SF2">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06694">
        <property type="entry name" value="Plant_NMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028655">
        <property type="entry name" value="Plant_NMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK10794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="37326" checksum="9BF6242461BD4B43" modified="2012-06-13" version="1">MASKQMEEIQRKLALLAYPRAKAPAQSLLFAGVERYRLLEWLFFRLLGDRSPFTQQNWQGDSLDRDEENSRIQHLAEIANFLGITPSVDTEAIQGRGSYDERVELLRLIVDLVEASCYADNPEWSVDEQLAKDVQLVDSIAEKQAQIFSEECKLFPADVQIQSIYPLPDIAELELKLSEYTKKMSNLQQMVQELASKYDYNPNEDYAETELKLREYLQSFLETVKSFNMIYTKEIHPWTHMMEVPQLHGFGPAANRLLEAYNTLLKFLGNLRSLRDSYSAMAAGSLSNSNEPSSVTKIISDCESALTFLNNSLAILSTSVAREQGETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1HU21</accession>
    <name>I1HU21_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100825394</name>
        <name evidence="3" type="ORF">BRADI_2g57120v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564786.1">
        <property type="nucleotide sequence ID" value="XM_003564738.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HU21"/>
    <dbReference type="FunCoup" id="I1HU21">
        <property type="interactions" value="525"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HU21"/>
    <dbReference type="EnsemblPlants" id="KQK10933">
        <property type="protein sequence ID" value="KQK10933"/>
        <property type="gene ID" value="BRADI_2g57120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825394"/>
    <dbReference type="Gramene" id="KQK10933">
        <property type="protein sequence ID" value="KQK10933"/>
        <property type="gene ID" value="BRADI_2g57120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825394"/>
    <dbReference type="eggNOG" id="ENOG502QR2C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1144292_0_0_1"/>
    <dbReference type="OMA" id="QEWEMIP"/>
    <dbReference type="OrthoDB" id="1931521at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045883">
        <property type="entry name" value="At4g13530-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33646">
        <property type="entry name" value="GB|AAF00631.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33646:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK10933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="244" mass="26074" checksum="12669A66E42B182B" modified="2012-06-13" version="1">MDGGAAADLQEWELLLASPTAGAAVSGPYAGGGGEGHDDVDAGAIKYDYFDLGSDARYTRRASLSIGGEEETDEEDALLDASGNASWVEPQPDDLAFPGRDRAALWSDSSSDGERREEAEATEPLEPPPPQEAAPAPADEGAVAKGGAAPWWKLPMEVLRLWASRAARSAWSLPVAVALLGIAVLGRRLYRMRRQSKAVARFRLVLDEKKASQFKAQPMLRRAPIVKPLLPANGVTPWPVLGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUK5</accession>
    <name>I1HUK5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Initiator tRNA phosphoribosyl transferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100844067</name>
        <name evidence="4" type="ORF">BRADI_2g58742v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753677.1">
        <property type="nucleotide sequence ID" value="XM_014898191.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUK5"/>
    <dbReference type="STRING" id="15368.I1HUK5"/>
    <dbReference type="EnsemblPlants" id="KQK11203">
        <property type="protein sequence ID" value="KQK11203"/>
        <property type="gene ID" value="BRADI_2g58742v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844067"/>
    <dbReference type="Gramene" id="KQK11203">
        <property type="protein sequence ID" value="KQK11203"/>
        <property type="gene ID" value="BRADI_2g58742v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2634">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027654_1_1_1"/>
    <dbReference type="OMA" id="PVFWANQ"/>
    <dbReference type="OrthoDB" id="45256at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HUK5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043399">
        <property type="term" value="F:tRNA adenosine(64)-2'-O-ribosylphosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019988">
        <property type="term" value="P:charged-tRNA amino acid modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000093">
        <property type="entry name" value="Initiator tRNA phosphoribosyl transferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007306">
        <property type="entry name" value="Rit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033421">
        <property type="entry name" value="Rit1_DUSP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033449">
        <property type="entry name" value="Rit1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811:SF0">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04179">
        <property type="entry name" value="Init_tRNA_PT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17184">
        <property type="entry name" value="Rit1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007747">
        <property type="entry name" value="Ribosyl_Ptfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rit1 N-terminal" evidence="3">
        <location>
            <begin position="26"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Rit1 DUSP-like" evidence="2">
        <location>
            <begin position="429"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK11203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="60625" checksum="10E8F9D59972F791" modified="2012-06-13" version="1">MAAAAAEAEPATEEGTLSIYKAARRIKRRGSTLYNALRSVAEDAAFVAEIAALWPALPLVANLRCGLWYTQPRTLAATCYFKSTDGHAGNWSFSTARLNLHLALLAGERGGCIIVDSTRKGKRFPDSMSKTIPIWCSVLNRAIQRHRLRASNQGSRTNSEMSAAAPNGNGEKYSGSSNWDSSVHLPVWVLDTEKNAIEGRVEEWTDRFESCGADIHSLALGLHKPLRPLWISQNTCIWLNEVPEHELWEFTPIILVSASASGAVATQRMSSEFSWHYIPGAGDDEESWARGLTPALFWKHSYDLLDGGPDLCNQLVADIVEKDRVYRAQRGERSPQITVNHLKCSGDDGPYFYEEHTIITKPMNSDPSIITPIDTPCSNNSHLVVFWIGRSNLAVSSTIQVAVDLADVDCILNCDSTSRLPSSSSENSYLEIAIVGSKNDRFSLLKNLPKAINFAQRNLIARRKILLCCQTGEDISICVALAIITRLFNDSGCFDDGDSFVKRNITKLEMRKRLVFICKYAINARPSRGNLRQVYGFLCNEKAQLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUP1</accession>
    <name>I1HUP1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100823360</name>
        <name evidence="6" type="ORF">BRADI_2g59070v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564950.1">
        <property type="nucleotide sequence ID" value="XM_003564902.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUP1"/>
    <dbReference type="STRING" id="15368.I1HUP1"/>
    <dbReference type="EnsemblPlants" id="KQK11260">
        <property type="protein sequence ID" value="KQK11260"/>
        <property type="gene ID" value="BRADI_2g59070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823360"/>
    <dbReference type="Gramene" id="KQK11260">
        <property type="protein sequence ID" value="KQK11260"/>
        <property type="gene ID" value="BRADI_2g59070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823360"/>
    <dbReference type="eggNOG" id="KOG1764">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036145_0_0_1"/>
    <dbReference type="OMA" id="MSIITCR"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF93">
        <property type="entry name" value="OJ1485_B09.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="284"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="362"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK11260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="437" mass="47818" checksum="C8A0E86C0C6C1DE5" modified="2012-06-13" version="1">MQARREIRTREERRDDEHGPVPETPLGSARWSPEAEIGMRVEDIWDSLEQSPEQQQLSRGDKLNSCFDAIPVSSFPQTFGSQLVDIPSDATLAEAVDILSRNRINGAPVRNVDAPDDASWIDRYIGIVEFAGIAVWLLHQSEAGAGAALGADELAAKLGTVALEGPKAQPPAAASMEAEAAVAEAFGSLPSSELFKRTKVKDISGSFRWAPFLALQSSDTFLTMLLLLSKYRMKSLPVVDIGEGTISNIITQAAVVHMLSECVGLSWFEDWGTKTLSDLGLPIMKPSRLVKVREDESALKAFRQMRKRGVGGIPVVDGADKPMGSIMIKDVKHLLTASEANRDYRTLTAKEFIASARQSSGEKQMSIITCTGDDTVKDIILRLDAEKRQRIYVVDAEGNLDGLITLRDIIAKLVYEPPGYFGDFFNGVIPMPQNSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HV95</accession>
    <name>I1HV95_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Uridine 5'-monophosphate synthase</fullName>
            <ecNumber evidence="5">2.4.2.10</ecNumber>
            <ecNumber evidence="6">4.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100836627</name>
        <name evidence="19" type="ORF">BRADI_2g60932v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>orotidine 5'-phosphate + H(+) = UMP + CO2</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the OMP decarboxylase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="5"/>
    <dbReference type="EC" id="4.1.1.23" evidence="6"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565082.1">
        <property type="nucleotide sequence ID" value="XM_003565034.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HV95"/>
    <dbReference type="FunCoup" id="I1HV95">
        <property type="interactions" value="2770"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HV95"/>
    <dbReference type="EnsemblPlants" id="KQK11573">
        <property type="protein sequence ID" value="KQK11573"/>
        <property type="gene ID" value="BRADI_2g60932v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836627"/>
    <dbReference type="Gramene" id="KQK11573">
        <property type="protein sequence ID" value="KQK11573"/>
        <property type="gene ID" value="BRADI_2g60932v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836627"/>
    <dbReference type="eggNOG" id="KOG1377">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049275_1_0_1"/>
    <dbReference type="OMA" id="SAKHVCG"/>
    <dbReference type="OrthoDB" id="10263753at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006222">
        <property type="term" value="P:UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000092">
        <property type="entry name" value="Uridine monophosphate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000025">
        <property type="entry name" value="Uridine monophosphate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF9">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="18">
        <location>
            <begin position="244"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="16">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="16">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="16">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQK11573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="475" mass="50828" checksum="058067A5EE2DEA61" modified="2012-06-13" version="1">MDAAALEALILDLHAIEAVKLGSFVLKSGITSPIYLDLRALVSHPRLLSSIASLLHALPSTRPYDILCGVPYTALPIASVLSVDRSVPMLLRRKEVKSHGTAKAIEGSFRAGDTVLIIEDLVTSGASVLETAAPLRAEGLVVSDAVVVVDREQGGRENLAANGITLHSLMTLTEVLAVLLKHGKVSEEKAGEVKQFLDANRKVTVPGSAVKPKVVRKGYAERAGLTKNPMGRKLFEVMEAKQSNLCVSADVGTAKELLELADKVGSEICMLKTHVDILSDFTPDFGSKLRSIAEKHNFLIFEDRKFADIGNTVTMQYEGGIFRILDWADIVNAHIIPGPGIVDGLKLKGLPKGRGLLLLAEMSSAGNLANGDYTAAAVKIAEQHSDFVIGFISVNPASWSVAPSSPAFIHATPGVQMVSGGDALGQQYTTPYSVINERGSDIIIVGRGIIKASDPYETARDYRIQGWQAYQSSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HVQ2</accession>
    <name>I1HVQ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100841734</name>
        <name evidence="12" type="ORF">BRADI_2g62290v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567536.1">
        <property type="nucleotide sequence ID" value="XM_003567488.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HVQ2"/>
    <dbReference type="FunCoup" id="I1HVQ2">
        <property type="interactions" value="1247"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK11777">
        <property type="protein sequence ID" value="KQK11777"/>
        <property type="gene ID" value="BRADI_2g62290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841734"/>
    <dbReference type="Gramene" id="KQK11777">
        <property type="protein sequence ID" value="KQK11777"/>
        <property type="gene ID" value="BRADI_2g62290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841734"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_21_1_1"/>
    <dbReference type="OMA" id="KAESPCN"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000258">
        <property type="entry name" value="Probable serine/threonine-protein kinase PBL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000228">
        <property type="entry name" value="Serine/threonine-protein kinase BIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="62"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK11777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="401" mass="43631" checksum="26ADFDD8359C46AB" modified="2012-06-13" version="1">MGNCFGTRISSDSPYRSSAASPSSGDAKSAELRAPRSEGEILQSAKVKSFAFTELRTATRNFRPDSVLGEGGFGSVFKGWIDENTFAPARPGTGVVIAVKKLNQDGFQGHREWLAEVNYLGQLSHPNLVKLVGYCLEDEQRLLVYEFMPRGSLENHLFRRGSHFQPLSWNLRMKVALGAAKGLAFLHSDKAKVIYRDFKTSNVLLDSSYNAKLSDFGLAKDGPTGDKSHVSTRVMGTHGYAAPEYLATGHLTAKSDVYSFGVVLLELLSGRRALDKNRPSGEHNLVEWARPYLTSKRRIFRILDARLGGQYSLAGAQKAAALALQCLSGDPRNRPGMEQVVAALGQLQDAAAKEAPPSAGGGGFVRMRGGKAETAAAAAGGWHRRQHQQYGRRMPALPLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1HWP9</accession>
    <name>I1HWP9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100844388</name>
        <name evidence="8" type="ORF">BRADI_3g02487v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573828.1">
        <property type="nucleotide sequence ID" value="XM_003573780.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HWP9"/>
    <dbReference type="FunCoup" id="I1HWP9">
        <property type="interactions" value="818"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HWP9"/>
    <dbReference type="EnsemblPlants" id="KQJ93057">
        <property type="protein sequence ID" value="KQJ93057"/>
        <property type="gene ID" value="BRADI_3g02487v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844388"/>
    <dbReference type="Gramene" id="KQJ93057">
        <property type="protein sequence ID" value="KQJ93057"/>
        <property type="gene ID" value="BRADI_3g02487v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844388"/>
    <dbReference type="eggNOG" id="KOG0454">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="WDDHVVR"/>
    <dbReference type="OrthoDB" id="2279155at2759"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HWP9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000010">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001614">
        <property type="entry name" value="PRK00402.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF2">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="7">
        <location>
            <begin position="79"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="7">
        <location>
            <begin position="369"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="20"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ93057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="516" mass="55883" checksum="A695B909CED7EC3F" modified="2012-06-13" version="1">MASISAAAKASAASFAHKKELAAAAPHQHQQQQRAGAAHQGHRRARVGGRVRAVATPARAPRAPASTGSVKAPMTTTEKILARASERARLEPGENVWVDVDVLMTHDVCGPGTIGIFKQEFGDDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCTEQEIKYFYDIRDLSDFRANPDYKGVCHIALAQEGHCRPGEVLLGTDSHTCNAGAFGQFATGIGNTDAGFVMGTGKALLKVPPTIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLTMEERMTLCNMVIEAGGKNGVVPADKTTFKYLEGKTSVEYEPVYSDAQARFFSDYRFDVSKLEPVVAKPHSPDNRALARECKDVKIDRVYIGSCTGGKTEDFLAAAKVFLASGKKVKVPTFLVPATQKVWMDVYSLPVPGSGGKTCSQIFEEAGCDTPASPNCGACLGGPRDTYARMNEPTVCVSTTNRNFPGRMGHKEGQIYLASPYTAAASALTGYVTDPRDFLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I1HWT1</accession>
    <name>I1HWT1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100830342</name>
        <name evidence="1" type="ORF">BRADI_3g02720v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573942.1">
        <property type="nucleotide sequence ID" value="XM_003573894.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HWT1"/>
    <dbReference type="FunCoup" id="I1HWT1">
        <property type="interactions" value="1781"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HWT1"/>
    <dbReference type="EnsemblPlants" id="KQJ93091">
        <property type="protein sequence ID" value="KQJ93091"/>
        <property type="gene ID" value="BRADI_3g02720v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830342"/>
    <dbReference type="Gramene" id="KQJ93091">
        <property type="protein sequence ID" value="KQJ93091"/>
        <property type="gene ID" value="BRADI_3g02720v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830342"/>
    <dbReference type="eggNOG" id="ENOG502S1Z4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117956_0_0_1"/>
    <dbReference type="OMA" id="QGLRWHA"/>
    <dbReference type="OrthoDB" id="550279at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35750">
        <property type="entry name" value="PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35750:SF1">
        <property type="entry name" value="PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ93091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="164" mass="17543" checksum="2B086992C4FCBC24" modified="2012-06-13" version="1">MGLFRRIAGMLGMSRDDADHHDAVAAAAGTGGGDVPHDKVAAAAAAAAAAGHQTRRGFSVQVPVPVDRPAAGPVLMPCAPGDGGVQGFRWYTRRLRIDEDGDVADEFLEEVLPESLINNDANPVGRFQVKYNTRPTALAMRKQTIAIDGDIRHSLEYQGQLQWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZP3</accession>
    <name>I1HZP3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100823996</name>
        <name evidence="3" type="ORF">BRADI_3g10760v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572746.1">
        <property type="nucleotide sequence ID" value="XM_003572698.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZP3"/>
    <dbReference type="FunCoup" id="I1HZP3">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HZP3"/>
    <dbReference type="EnsemblPlants" id="KQJ94476">
        <property type="protein sequence ID" value="KQJ94476"/>
        <property type="gene ID" value="BRADI_3g10760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823996"/>
    <dbReference type="Gramene" id="KQJ94476">
        <property type="protein sequence ID" value="KQJ94476"/>
        <property type="gene ID" value="BRADI_3g10760v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823996"/>
    <dbReference type="eggNOG" id="ENOG502QPWD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094750_0_0_1"/>
    <dbReference type="OMA" id="VQVRVWN"/>
    <dbReference type="OrthoDB" id="1912879at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HZP3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37200">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37200:SF1">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094921" description="RRM domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ94476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="28011" checksum="39FEE57AF76BCFBA" modified="2012-06-13" version="1">MPPLLLLPSACCAAVAAAAPSSASAAFSRAPPSRLRPSHLPFAFRRLRKYGRRDREPVASTSSDEEESDDDGVEEAVDEDIFLKNRPKPAGFGVGKTYTTDIEERLLREMGLGGSGRKSEPAPAKHLIRAADSAKETGGDSTDGIHVRIWNLPKKKNIHKDLNLAFKRFPGLITINPANSANKKTRDPVCKGFAFLKMESLEAATRFVELYSSKSVLFGKVEKSIKCCIVDGHSSTDTSNKPSSTKAAGSTLQRLAAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1I0A4</accession>
    <name>I1I0A4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100825016</name>
        <name evidence="11" type="ORF">BRADI_3g13090v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573257.1">
        <property type="nucleotide sequence ID" value="XM_003573209.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I0A4"/>
    <dbReference type="STRING" id="15368.I1I0A4"/>
    <dbReference type="EnsemblPlants" id="KQJ94775">
        <property type="protein sequence ID" value="KQJ94775"/>
        <property type="gene ID" value="BRADI_3g13090v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66492">
        <property type="protein sequence ID" value="PNT66492"/>
        <property type="gene ID" value="BRADI_3g13090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825016"/>
    <dbReference type="Gramene" id="KQJ94775">
        <property type="protein sequence ID" value="KQJ94775"/>
        <property type="gene ID" value="BRADI_3g13090v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66492">
        <property type="protein sequence ID" value="PNT66492"/>
        <property type="gene ID" value="BRADI_3g13090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825016"/>
    <dbReference type="eggNOG" id="KOG1311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018741_2_1_1"/>
    <dbReference type="OMA" id="AIMAYCF"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I0A4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF113">
        <property type="entry name" value="S-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="10">
        <location>
            <begin position="132"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="334"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ94775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="410" mass="46421" checksum="28FBEDE087BB2C2D" modified="2012-06-13" version="1">MAKQQRVYQVWRGNNIIWCHGRLIFGPDAKATLLSFALIASPVVVFCIFVARHLVHFFPAYNAGYAIPVVTIVLTVHVLLLLFFTSSQDPGIVPRNSHPPVEEFSHDASAPHTLQFPRIKEVMVNGIPVRVKYCETCMIYRPPRCSHCSKCDNCVERFDHHCPWVGQCIGQRNYRYFFWFVSSAAVLCFYVFSMCALYISLIMKRGHHSVVEAIKESPASVAVMAYCFICFWFVGGLTGFHSYLIATNKTTYENLKYKYNNQPNAFDRGCMHNCFEVLCTKRKPSRINLRGIVQEEHGATLPRISRSSVPEDETPHRPRAKVEDDLEMGLDILKTSRRRSDELSDGELGAESNGVKYRRGDCTPDSDTEIPVIRSTAESSNEVRDLDILSVGNAARPSSSGQKHRPDELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1B0</accession>
    <name>I1I1B0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Polyamine transporter PUT1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Polyamine uptake transporter 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100830035</name>
        <name evidence="13" type="ORF">BRADI_3g16160v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Polyamine:cation symporter (PHS) (TC 2.A.3.12) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573441.1">
        <property type="nucleotide sequence ID" value="XM_003573393.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1B0"/>
    <dbReference type="STRING" id="15368.I1I1B0"/>
    <dbReference type="EnsemblPlants" id="KQJ95270">
        <property type="protein sequence ID" value="KQJ95270"/>
        <property type="gene ID" value="BRADI_3g16160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830035"/>
    <dbReference type="Gramene" id="KQJ95270">
        <property type="protein sequence ID" value="KQJ95270"/>
        <property type="gene ID" value="BRADI_3g16160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830035"/>
    <dbReference type="eggNOG" id="KOG1287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_17_3_1"/>
    <dbReference type="OMA" id="GPSRGMF"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I1B0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000041">
        <property type="entry name" value="Amino acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044566">
        <property type="entry name" value="RMV1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826:SF2">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826">
        <property type="entry name" value="POLYAMINE TRANSPORTER PUT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ95270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="495" mass="54557" checksum="0D16AF05EAC2AB9E" modified="2012-06-13" version="1">MKLRSTAITRANSACLPMEDRGCFKYNGVNEGEEERKGGHGIPKVSMVPLIFLIFYEVSGGPFGIEDSVKAAGPLLAIVGFLLFALIWSVPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWAKWISGVIDNALYPVLFLDYVKSSIPALGGGLPRTFAVLILTVALTYMNYRGLTIVGWVAVFLGVFSLLPFFVMGLIAIPRIEPSRWLEMDLSNVNWSLYLNTLFWNLNYWDSISTLAGEVENPKRTLPKALSYALVLVVGGYLYPLITCTAALPVVRESWTDGYFSDVAKILGGFWLHSWIQAAAALSNMGNFVTEMSSDSYQLLGMAERGMLPEFFAKRSRHGTPLVGILFSAFGVVLLSWMSFQEIIAAENYLYCFGMILEFIAFIKLRMTHPNTSRPYRIPLGTVGAVLMIIPPAILIIVVMALASFKVMAVSILAVLIGFALQPSLVYVEKKRWLRFSVSEDLPELPDSSPVADGDETVPLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1J6</accession>
    <name>I1I1J6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm-like protein LSM2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100843890</name>
        <name evidence="11" type="ORF">BRADI_3g16900v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of LSM protein complexes, which are involved in RNA processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573488.1">
        <property type="nucleotide sequence ID" value="XM_003573440.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1J6"/>
    <dbReference type="SMR" id="I1I1J6"/>
    <dbReference type="STRING" id="15368.I1I1J6"/>
    <dbReference type="EnsemblPlants" id="KQJ95388">
        <property type="protein sequence ID" value="KQJ95388"/>
        <property type="gene ID" value="BRADI_3g16900v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ95389">
        <property type="protein sequence ID" value="KQJ95389"/>
        <property type="gene ID" value="BRADI_3g16900v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66786">
        <property type="protein sequence ID" value="PNT66786"/>
        <property type="gene ID" value="BRADI_3g16900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843890"/>
    <dbReference type="Gramene" id="KQJ95388">
        <property type="protein sequence ID" value="KQJ95388"/>
        <property type="gene ID" value="BRADI_3g16900v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95389">
        <property type="protein sequence ID" value="KQJ95389"/>
        <property type="gene ID" value="BRADI_3g16900v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66786">
        <property type="protein sequence ID" value="PNT66786"/>
        <property type="gene ID" value="BRADI_3g16900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843890"/>
    <dbReference type="eggNOG" id="KOG3448">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130474_3_0_1"/>
    <dbReference type="OMA" id="DNISCTD"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01725">
        <property type="entry name" value="LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000009">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016654">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829">
        <property type="entry name" value="SNRNP CORE PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829:SF2">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016394">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT66786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="93" mass="10737" checksum="501F1FFDACCC5F34" modified="2012-06-13" version="1">MLFFSYFKELVGKEVTVELKNDLAIRGTLHSVDQYLNIKLENTRVVDQDKYPHMLSVRNCFIRGSVVRYVLLPQDGVDIDILHDATRREARGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2D0</accession>
    <name>I1I2D0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100831868</name>
        <name evidence="14" type="ORF">BRADI_3g19170v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573619.1">
        <property type="nucleotide sequence ID" value="XM_003573571.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2D0"/>
    <dbReference type="STRING" id="15368.I1I2D0"/>
    <dbReference type="EnsemblPlants" id="KQJ95809">
        <property type="protein sequence ID" value="KQJ95809"/>
        <property type="gene ID" value="BRADI_3g19170v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ95811">
        <property type="protein sequence ID" value="KQJ95811"/>
        <property type="gene ID" value="BRADI_3g19170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831868"/>
    <dbReference type="Gramene" id="KQJ95809">
        <property type="protein sequence ID" value="KQJ95809"/>
        <property type="gene ID" value="BRADI_3g19170v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95811">
        <property type="protein sequence ID" value="KQJ95811"/>
        <property type="gene ID" value="BRADI_3g19170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831868"/>
    <dbReference type="eggNOG" id="ENOG502QR1Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="ARSWHKN"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I2D0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056760">
        <property type="entry name" value="RING_XB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24921">
        <property type="entry name" value="RING_XB3-XBAT31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="83"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="223"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="321"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ95809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="485" mass="52034" checksum="BF6443FFEBEE5B8F" modified="2012-06-13" version="1">MGILGMMGDSFGCSATGERLVSAARDGDIQEARALLELNPRLARYSTFGIRNSPLHYSAAKGHHEIVSLLIESGVDINLRNCRGQTALMQACLYGHWKVVQILVLFKANIHKKDCFSGASAIHFAALKGHTRCIRLLVADYVPSMPNFWNIMHGLSTEERNKEAYDAGTLRRLLNGKSDGGVTPLHLAALHGHAESLQLLLDLGASVSEVTVNDGSTIDLIGSGSTPLHYAACGGSAVCCQLLIAAGANMAAENANGLTPLMVARSWHKTSIESILSKQPEGRLRILPSPYLSLPLMSIVKIARECGWRKTSVSSTCHDPCVICLEMECTVAAEGCGHEFCTKCALYLCSTTSSSTSIRGVPGSISCPLCRQAIVSFMKLTSTTPIKELPWTSSSLALCAAGASTTGSKCPSSLHRRSDMNRLRSSSVHLGCSSFRSIGSGKLSSIKLNCGGLDETMPCLVSCLRPDARRSSSYRERISRYSEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1I7C4</accession>
    <name>I1I7C4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100838603</name>
        <name evidence="10" type="ORF">BRADI_3g36740v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
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            <strain evidence="10 11">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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</entry>
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        <lineage>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">Bd21</strain>
        </source>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="5">cv. Bd21</strain>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="312" mass="34608" checksum="E5EEBDD819BE7355" modified="2012-06-13" version="1">MPPPETEPRISSIVGCRAAATAGRRRHRSPSPDRDRHRRHHEGDGQGLKGSSLARRVSDSSESDGSRSDGRHRRRCKERGRGRRGKESRRRHRGRSRGSDDDDSEISYDSVGSGDGKRRKRSRRSSRNGSRSRRKKSRGSDSHGSGKPSGGARSNVKTSDEPKEALEFREMIEEAPPGPMPMPQPRAEAQIVKYGGALRPGEGDAMAQFVQQGKRIPRRGEVGLTAEEIQRFEAAGYVMSGRAGTRGSPPCASARRTRCTAPRRSARALATFNYEQKAARERKVRDDLRRLVDRALGKLAQTAEQHDPFADK</sequence>
</entry>
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    <accession>I1I8A8</accession>
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        <recommendedName>
            <fullName evidence="3">BURP domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        </lineage>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235278.1">
        <property type="nucleotide sequence ID" value="XM_010236976.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8A8"/>
    <dbReference type="FunCoup" id="I1I8A8">
        <property type="interactions" value="390"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I8A8"/>
    <dbReference type="EnsemblPlants" id="KQJ98849">
        <property type="protein sequence ID" value="KQJ98849"/>
        <property type="gene ID" value="BRADI_3g39490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827283"/>
    <dbReference type="Gramene" id="KQJ98849">
        <property type="protein sequence ID" value="KQJ98849"/>
        <property type="gene ID" value="BRADI_3g39490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827283"/>
    <dbReference type="eggNOG" id="ENOG502QQHP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011822_1_0_1"/>
    <dbReference type="OMA" id="TLRTCEW"/>
    <dbReference type="OrthoDB" id="1909293at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009555">
        <property type="term" value="P:pollen development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044816">
        <property type="entry name" value="BURP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004873">
        <property type="entry name" value="BURP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31236">
        <property type="entry name" value="BURP DOMAIN PROTEIN USPL1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31236:SF10">
        <property type="entry name" value="BURP DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03181">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01045">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51277">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095173" description="BURP domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="BURP" evidence="3">
        <location>
            <begin position="218"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ98849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="441" mass="46578" checksum="C45725C83DA0B5A0" modified="2012-06-13" version="1">MARFLVLAFLAASLVAAGWPLGQAAPAKSTAEVFWRAALPDSPLPDAILRLLRPAPAAESGFVSRGAEDEEATNKPPFNYQDYKRPSGNGAASLAGDSPFGYKYQALSSSGKRAAAVGAARDFDYDDYSASEEEKRAGTGKQMAPSGYDYKKAAGERRRRAAAPPPSPFMYAYKTTSATAGNGKPKPPKPAPSPFMYAYVKKKASTATGANGKTTTVFFHEEAVRVGERLRFHFPPASPAPLGLLPRHVADSIPFSPGSLPAALATLGVAPDSAMARRMEATLSTCDDEASPAITGESKFCAASLEALVEGAMASLGTRDIRPLTSRVPRAGAPAQTYAVRAVRAVEGGPVFVACHDEPYPYAVYRCHTTGPARAYVVDMDGEDGGDKVTLAAVCHTDTSLWNPDHVSFQLLATKPGGAPVCHLMPYGHVLWGKDVKRSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IAV8</accession>
    <name>I1IAV8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100838110</name>
        <name evidence="6" type="ORF">BRADI_3g46810v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575220.1">
        <property type="nucleotide sequence ID" value="XM_003575172.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IAV8"/>
    <dbReference type="STRING" id="15368.I1IAV8"/>
    <dbReference type="EnsemblPlants" id="KQK00021">
        <property type="protein sequence ID" value="KQK00021"/>
        <property type="gene ID" value="BRADI_3g46810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838110"/>
    <dbReference type="Gramene" id="KQK00021">
        <property type="protein sequence ID" value="KQK00021"/>
        <property type="gene ID" value="BRADI_3g46810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838110"/>
    <dbReference type="eggNOG" id="KOG1609">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061276_1_2_1"/>
    <dbReference type="OMA" id="DEWENGV"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IAV8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000146">
        <property type="entry name" value="RING/FYVE/PHD zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022143">
        <property type="entry name" value="DUF3675"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033275">
        <property type="entry name" value="MARCH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012:SF200">
        <property type="entry name" value="OS02G0577100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12428">
        <property type="entry name" value="DUF3675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="5">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK00021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="259" mass="28820" checksum="874987DF6CE6328D" modified="2012-06-13" version="1">MGDHVALDVRGFVPSRAGEGLGLASCGEEVEALIGMVECRICQEEDLAKNLESPCACSGSLKYAHRECVQRWCNEKGDIICEICHELYKPGYTAPPQIHHDETTIEISGDWSISGNHLDLHDPRILAMAAAQHRLLEDEYDEYTATNNNAAAFCRSIFLILMALLLLRHTLTITSSDDEDDASAIFSLFLLRAAGFLLPCYIMAWAISIMQRQRQRQEEAMLLPTEVAIILHRNGRMMQFAVAPESPASPHPELPQLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICR5</accession>
    <name>I1ICR5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100829099</name>
        <name evidence="8" type="ORF">BRADI_3g52070v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the purine permeases (TC 2.A.7.14) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570128.1">
        <property type="nucleotide sequence ID" value="XM_003570080.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICR5"/>
    <dbReference type="STRING" id="15368.I1ICR5"/>
    <dbReference type="EnsemblPlants" id="KQK00826">
        <property type="protein sequence ID" value="KQK00826"/>
        <property type="gene ID" value="BRADI_3g52070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829099"/>
    <dbReference type="Gramene" id="KQK00826">
        <property type="protein sequence ID" value="KQK00826"/>
        <property type="gene ID" value="BRADI_3g52070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829099"/>
    <dbReference type="HOGENOM" id="CLU_043459_2_1_1"/>
    <dbReference type="OrthoDB" id="1907510at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ICR5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015211">
        <property type="term" value="F:purine nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030182">
        <property type="entry name" value="PUP_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376">
        <property type="entry name" value="OS09G0467300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376:SF2">
        <property type="entry name" value="PURINE PERMEASE 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16913">
        <property type="entry name" value="PUNUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">cv. Bd21</strain>
        </source>
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    <sequence length="357" mass="38452" checksum="6E14C0061A497A66" modified="2012-06-13" version="1">MATESSTLPLPPRLSASPASSPATVGAFLTKTGGAPRDACRSPRSLLSRILGRGGGGFGCRFRLPRYCSGGASGKEYAGEEDEAGWTRAPQPQVVGGHAAEARESPPRSSFGKKAAAAASLGLGAGLVLLLSKGAAELSRMEELRAQMERLVLDAKVAARRGGSSSNDADESASVVIEPTVFAGAGEEDASSHCSRTAAAAMDQMEAELEAELTRLQLASNEDDEECATPRQDHQLLDAEAESEVASENQSPACVLQNGSAIIDDGAARSEHGREEDEDNEAEEEEERVACHTGVPARELERRLHELLQSRHEERIAELESELERARRKLREKEREVSRWRDTAKLVSRREDESRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDR4</accession>
    <name>I1IDR4_BRADI</name>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="10">
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQK01284">
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        <property type="gene ID" value="BRADI_3g54920v3"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="GO_Central"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
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        <location>
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            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="727"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="2"/>
            <end position="361"/>
        </location>
    </feature>
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            <end position="807"/>
        </location>
    </feature>
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    </feature>
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    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
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            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK01284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="807" mass="90904" checksum="738C8A818DB4A230" modified="2012-06-13" version="1">MVKFSREYEASIIPEWKAAFVDYKGLKKLIKKIKISRRDDDSSTSSALIASSGAGDRGESESESFSFSVLDPVRALAARLAPRVQALGPNNSEDEEGSGTDSGELVRSWDKHEREFLERADEELEKVNSFYGAQEAELLARGDALLEQLRILADVKRILADHAANRRRNRGLARTRSMPTPPPLSASPNVSSGRYLLSPGLASPQSMSDGSLEMQQAETAEGAAVADEVMAALERNGVSFLGLPGKKDAKKADAGAKSRALQMPATVRIDIPASNPGRTALKVWEELVNVLRKDGADPAAAFVHRKKVQHAEKNIRDAFMALYRGLELLKKFSSLNVKAFTKILKKFVKVSEQQRATDLFSQKVKRSPFSSSDKVLQLADEVESLFTKNFTGNDRMVAMKYLKPQQLKNTHMITFLVGLFTGTFVSLFIIYAILAHVSGIFASAGNTAYMEVVFHVFSMFALISLHCFLYGCNLFMWKSTRINQNFIFDFAPNTALTHRDAFLMSASIMCTVVAALVINLFLRNAGASYANAVPGGLLVLSIGVLFCPFNVFYRSTRYCFMRILRNIIFSPFYKVLMADFFMADQLTSQVPLLRHMEFAACYFMAGSFKANPYETCTNGQQYKHIAYVISFLPYYWRAMQCLRRYLEEHDMNQLANAGKYVSAMVAAAVKFKYAATPTPFWVLMVVISSSGATSYQLYWDFVKDWGFFTPKSKNLWLRDELILKNKSIYYLSMVLNLLLRLAWTESVMKFRVGKVETRLLDFSLASLEIIRRGHWNFYRLENEHLNNVGKFRAVKTVPLPFRELETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1IE94</accession>
    <name>I1IE94_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100831353</name>
        <name evidence="13" type="ORF">BRADI_3g56210v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572982.1">
        <property type="nucleotide sequence ID" value="XM_003572934.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IE94"/>
    <dbReference type="FunCoup" id="I1IE94">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01497">
        <property type="protein sequence ID" value="KQK01497"/>
        <property type="gene ID" value="BRADI_3g56210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831353"/>
    <dbReference type="Gramene" id="KQK01497">
        <property type="protein sequence ID" value="KQK01497"/>
        <property type="gene ID" value="BRADI_3g56210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831353"/>
    <dbReference type="eggNOG" id="ENOG502SBRV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020953_6_1_1"/>
    <dbReference type="OMA" id="EFWTASA"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990538">
        <property type="term" value="F:xylan O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF39">
        <property type="entry name" value="OS02G0729200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="12">
        <location>
            <begin position="66"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="11">
        <location>
            <begin position="120"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK01497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="424" mass="47194" checksum="1CC0EEC8B8F89A18" modified="2012-06-13" version="1">MKPPNSQTAVRRAASTTGLLLILLLVAFTACNYTSLNLNTEEFIDLPAPRSVVRHNNDNNNNAGAACDVAKGEWVRDKAAPYYTNATCPLIDGRQDCMKYGKPGLASILQWRWQPHGCDLRRFDAAAFFRIVRGKSMAFVGDSVARNHMQSLMCLLSQTAGAGQPTEIGGAKDCVHCTRKYRYEEHGFTVAVFWTPFLVRWNLTRAGALQFMDPHNVFLDEPDPEWSGHVLDYDYVVLNGAKWFTRPTVLYERGRRLGCVNHECSAGADQDNAMAVAPEYAVRAAFRTALEALAGFRGTVVVRTVAPPHYENGKWYDGGDCVRRSPYRGNETRLPETEEAFHAAQVEEFRAAASEADQSNGDGKKGRLVLMDVSEMMQMRGDGHPGRYGHWPHEKVGFGIDCVHWCLPGPVDAWNELLLHLLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEN0</accession>
    <name>I1IEN0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC104583870</name>
        <name evidence="4" type="ORF">BRADI_3g57370v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236047.1">
        <property type="nucleotide sequence ID" value="XM_010237745.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEN0"/>
    <dbReference type="STRING" id="15368.I1IEN0"/>
    <dbReference type="EnsemblPlants" id="KQK01658">
        <property type="protein sequence ID" value="KQK01658"/>
        <property type="gene ID" value="BRADI_3g57370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583870"/>
    <dbReference type="Gramene" id="KQK01658">
        <property type="protein sequence ID" value="KQK01658"/>
        <property type="gene ID" value="BRADI_3g57370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583870"/>
    <dbReference type="eggNOG" id="ENOG502R1BM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058544_0_1_1"/>
    <dbReference type="OMA" id="MENFGMT"/>
    <dbReference type="OrthoDB" id="9442240at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009739">
        <property type="term" value="P:response to gibberellin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010090">
        <property type="term" value="P:trichome morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044291">
        <property type="entry name" value="GIS/GIS2/ZFP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46547:SF4">
        <property type="entry name" value="OS02G0775600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46547">
        <property type="entry name" value="ZINC FINGER PROTEIN GIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK01658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="286" mass="30373" checksum="304DD29ADCE0C2B5" modified="2012-06-13" version="1">MATPLEQVRSVDSFSQLPFIRQAVAAAPARDTTIRLFGHEFSNNNADERHQQHKESSAPGTGSPEYAANNNNGSTVTSDGGSGGKERKFECHYCCRNFPTSQALGGHQNAHKRERQHAKRAHLQASLAAMHHHHHRYAAVPAHHMYGALLGYPPPPPAHYSMWTTTTAAAGPPGSAYGLGPGSMAQPIDGSPVQGLWGPRPVQSFAGPHGPADMVPVMRPAAAGAQVVVDFKDDEKKAVMSLLSSSSSPPSLSSCSSTSAPEKIAAGRCELGQQRQEAVISLDLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEY0</accession>
    <name>I1IEY0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100838905</name>
        <name evidence="12" type="ORF">BRADI_3g58197v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570584.1">
        <property type="nucleotide sequence ID" value="XM_003570536.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEY0"/>
    <dbReference type="STRING" id="15368.I1IEY0"/>
    <dbReference type="EnsemblPlants" id="KQK01784">
        <property type="protein sequence ID" value="KQK01784"/>
        <property type="gene ID" value="BRADI_3g58197v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838905"/>
    <dbReference type="Gramene" id="KQK01784">
        <property type="protein sequence ID" value="KQK01784"/>
        <property type="gene ID" value="BRADI_3g58197v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838905"/>
    <dbReference type="eggNOG" id="KOG0546">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_37_3_1"/>
    <dbReference type="OMA" id="TKGSCIH"/>
    <dbReference type="OrthoDB" id="407558at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IEY0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000009">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF461">
        <property type="entry name" value="OS02G0761100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7 10" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="11">
        <location>
            <begin position="25"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="316"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK01784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="390" mass="42588" checksum="3F1F7B5C6F54626C" modified="2012-06-13" version="1">MEGEGSPVDAPAPAAAVEVKNPRCYLDVSIGGEMEGRIVMVLYASVAPRTAENFRALCTGEKGVSAATGARLHYKGSCFHRIIKGFMVQGGDITAGDGTGGESIYGLNFEDENFVLKHERKGMLSMANAGPNTNGSQFFITTTRTPHLDGKHVVFGRVVKGMGVVRAMEHISAGEADLPTADIVVVDCGELPEDANDGVVDFFKDGDMYPDWPNDLDEKPAEVSWWIDAVDSAKSFGNENFKKHDYKAALRKYRKAMRYLDLCWEKEEIDQEKSSALRKTKSIILTNSSACKLKLGDVEGALLDADFALRETEGNAKAFFRQGQARMALKNIDAAVESFKHALDLEPNDGGIKRELAAAKKKVADRRGLERKAFSKMFQSSGSSDKIDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJS9</accession>
    <name>I1IJS9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100826784</name>
        <name evidence="5" type="ORF">BRADI_4g11540v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237482.1">
        <property type="nucleotide sequence ID" value="XM_010239180.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJS9"/>
    <dbReference type="FunCoup" id="I1IJS9">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IJS9"/>
    <dbReference type="EnsemblPlants" id="KQJ87503">
        <property type="protein sequence ID" value="KQJ87503"/>
        <property type="gene ID" value="BRADI_4g11540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826784"/>
    <dbReference type="Gramene" id="KQJ87503">
        <property type="protein sequence ID" value="KQJ87503"/>
        <property type="gene ID" value="BRADI_4g11540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826784"/>
    <dbReference type="eggNOG" id="ENOG502S7A5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087111_1_1_1"/>
    <dbReference type="OMA" id="HTHTRVM"/>
    <dbReference type="OrthoDB" id="1864232at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982793" description="Dirigent protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ87503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="160" mass="16722" checksum="2C6FF7AD3FBE4E31" modified="2012-06-13" version="1">MLLALFLALAMAAHPAPASAASAHLHFYMHDVLTGASPTAVQVLNGPKGHFGDTIVIDDVLTASDSPSSALVGRAQGQYIWASRGNPELLVTMDVVLTSGAYAGSSVTVVGRDDIGAAVRELAVVGGTGQFRMARGYVLWKTVRLDHPNAVLELDVFVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1IL72</accession>
    <name>I1IL72_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Purple acid phosphatase</fullName>
            <ecNumber evidence="10">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100845233</name>
        <name evidence="14" type="ORF">BRADI_4g16600v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="11">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="10"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577477.1">
        <property type="nucleotide sequence ID" value="XM_003577429.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IL72"/>
    <dbReference type="FunCoup" id="I1IL72">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IL72"/>
    <dbReference type="EnsemblPlants" id="KQJ88251">
        <property type="protein sequence ID" value="KQJ88251"/>
        <property type="gene ID" value="BRADI_4g16600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845233"/>
    <dbReference type="Gramene" id="KQJ88251">
        <property type="protein sequence ID" value="KQJ88251"/>
        <property type="gene ID" value="BRADI_4g16600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845233"/>
    <dbReference type="eggNOG" id="KOG2679">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043332_3_0_1"/>
    <dbReference type="OMA" id="KICYFHH"/>
    <dbReference type="OrthoDB" id="411211at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IL72">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07378">
        <property type="entry name" value="MPP_ACP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000027">
        <property type="entry name" value="Purple acid phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024927">
        <property type="entry name" value="Acid_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051558">
        <property type="entry name" value="Metallophosphoesterase_PAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10161:SF19">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10161">
        <property type="entry name" value="TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000898">
        <property type="entry name" value="Acid_Ptase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095442" description="Purple acid phosphatase" evidence="12">
        <location>
            <begin position="27"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="13">
        <location>
            <begin position="42"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000898-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ88251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="331" mass="36277" checksum="73DBA676E00FB217" modified="2012-06-13" version="1">MASSAVLLITGVVVVAGALLLSQVEAELQKVQHSPKEHGSLTVLAVGDWGRAGQYNQTLVAEQMGVVGEKLSADFVISTGDNFYNDGLAGDNDTAFFRASFSDIYTADSLQKPWYIVLGNHDYTGDALAQQSPAIRDVDTRWTSVNKSFIVESGIVDFFLVDTSPFVLKYWNESKFDWRNVAPRDTYISNLLKDLEEALTASNATWKVVVGHHPISSGCEHGNTTELREYLLPVLKTHGVDMYLNGHDHCLQRTSSTDSPVEFVTSGGGSKAWAGKFKPTSDKMEFLYDGQGFLSMQLTAAEARFAFYDVSGEVLHAWELTKSPTGARIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1IL76</accession>
    <name>I1IL76_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100845844</name>
        <name evidence="16" type="ORF">BRADI_4g16630v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ADAGIO family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577479.1">
        <property type="nucleotide sequence ID" value="XM_003577431.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IL76"/>
    <dbReference type="FunCoup" id="I1IL76">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IL76"/>
    <dbReference type="EnsemblPlants" id="KQJ88263">
        <property type="protein sequence ID" value="KQJ88263"/>
        <property type="gene ID" value="BRADI_4g16630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845844"/>
    <dbReference type="Gramene" id="KQJ88263">
        <property type="protein sequence ID" value="KQJ88263"/>
        <property type="gene ID" value="BRADI_4g16630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845844"/>
    <dbReference type="eggNOG" id="ENOG502QWBU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033494_1_0_1"/>
    <dbReference type="OMA" id="AVCWRKF"/>
    <dbReference type="OrthoDB" id="447251at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IL76">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009902">
        <property type="term" value="P:chloroplast relocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001007">
        <property type="term" value="P:negative regulation of cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009911">
        <property type="term" value="P:positive regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22154">
        <property type="entry name" value="F-box_AtADO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000041">
        <property type="entry name" value="Adagio protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000005">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000026">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011498">
        <property type="entry name" value="Kelch_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46175:SF2">
        <property type="entry name" value="ADAGIO PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46175">
        <property type="entry name" value="BACTERIOOPSIN TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07646">
        <property type="entry name" value="Kelch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="11" id="KW-0157">Chromophore</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="PAS" evidence="15">
        <location>
            <begin position="54"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQJ88263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="621" mass="68450" checksum="373FA0AB904001C0" modified="2012-06-13" version="1">MFDAADHGAVVAVKRMRLCAAAEDMGMEVDGEEEEEEEEEGWAWGAPAAGPAGEQRAAAIVVADAVEPDFPVIYVNAAFEAATGYRAHEVLGRNCRFLQFRDPRAQRRHPLVDPMVVSEMRRCLNDGIEFEGELLNFRKDGAPLNNRLRLIPMHGDDGSMTHIIAIQLFSDANIDLSNISYPVYKQQSNRRPSIQDMNPASHEHTTKIQCSDYCGIFQLSDEVLAHNILSRLSPRDVASIGSVCTRMHQLTKNDHLRKMVCQNAWGRDVTVRLEMSTKMLGWGRLARELTTLEAASWRKFTVGGRVEPSRCNFSACAVGNRLVLFGGEGVNMQPMDDTFVLNLDAAKPEWRRVKVSASPPGRWGHTLTWLNGSWLVVFGGCGQQGLLNDVFVLDLDAQQPTWREVTSDGPPLPRSWHSSCTLDGSKLLVSGGCAESGVLLSDTFLLDLAKEKPAWKEIPTSWSSRLGHTMSVYGKSKLFMFGGMAKSGSLRLRSSDAYTMDVGEKNPQWRQLATTGFPSVGPPPRLDHVTVTLPCGRIIIFGGSIAGLHSPAELFLLDPAEEKPTWRILNVPGQPPKFAWGHSTCVVGGTRVLVLGGHTGEEWILNELHELCLASRPDEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>I1INL3</accession>
    <name>I1INL3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100831178</name>
        <name evidence="17" type="ORF">BRADI_4g25880v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577849.1">
        <property type="nucleotide sequence ID" value="XM_003577801.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INL3"/>
    <dbReference type="STRING" id="15368.I1INL3"/>
    <dbReference type="EnsemblPlants" id="KQJ89464">
        <property type="protein sequence ID" value="KQJ89464"/>
        <property type="gene ID" value="BRADI_4g25880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831178"/>
    <dbReference type="Gramene" id="KQJ89464">
        <property type="protein sequence ID" value="KQJ89464"/>
        <property type="gene ID" value="BRADI_4g25880v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_59_0_1"/>
    <dbReference type="OMA" id="MYRKIQN"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1INL3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000279">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000096">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF84">
        <property type="entry name" value="CBL-INTERACTING PROTEIN KINASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="12"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="NAF" evidence="16">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ89464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="444" mass="49828" checksum="A741ADACB6DBB7C2" modified="2012-06-13" version="1">MESRGKILMERYELGRLLGQGTFGKVHYARSLESNQSVAIKMLEKEKMLSAGLSEQIRREVTTMRLVAHKNVVQLHEVMATRNKIYFVMEYMKGGELFDKVEKGGKLTEVAAHKYFHQLIAAVDYCHSRGVYHRDLKPENLLLDDNENLKVSDFGLSALSESKRLDGLLHTTCGTPAYVAPEVISKRGYDGAKSDIWSCGVILFVLVAGYLPFEAPNLMDMYRKIEQGQFKCPSWLSQKLQKLLYKILDPNPSTRASIQKIKESSWFRKGPVGTLTVKGRTPSENATASAAPTAGVRRSRKKADGDATPLAVTNLNAFEIISFSTGFDLSGLFIEEECRKEARFTSDKPAAAIISKLECVAKALSLRVRKKDNGVVKMQARKEGRNGVVQFNAEIFEITASYHLIEMKQTSGDPLEYRGLLEDIRPALKDIVRSWHGDDKQQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IRH5</accession>
    <name>I1IRH5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carboxypeptidase</fullName>
            <ecNumber evidence="9">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100839940</name>
        <name evidence="10" type="ORF">BRADI_4g34320v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="9"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576701.1">
        <property type="nucleotide sequence ID" value="XM_003576653.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IRH5"/>
    <dbReference type="MEROPS" id="S10.A41"/>
    <dbReference type="EnsemblPlants" id="KQJ90882">
        <property type="protein sequence ID" value="KQJ90882"/>
        <property type="gene ID" value="BRADI_4g34320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839940"/>
    <dbReference type="Gramene" id="KQJ90882">
        <property type="protein sequence ID" value="KQJ90882"/>
        <property type="gene ID" value="BRADI_4g34320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839940"/>
    <dbReference type="eggNOG" id="KOG1282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008523_13_2_1"/>
    <dbReference type="OMA" id="YSIQDMT"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000001">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000573">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF59">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982820" description="Carboxypeptidase" evidence="9">
        <location>
            <begin position="26"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ90882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="497" mass="54466" checksum="ADF54E4C8F87145D" modified="2012-06-13" version="1">MKSLYSQLLLICVAAAALHLHAANASQEARLKAFISSRITGDSSSGGTFKARDITDRFAASLSAESSVSDQSSMKAADKITALPGQPKGVDFDQYSGYVTVDEENGRALFYYLVESPSGASEKPLVLWLNGGPGCSSLGYGAMQELGPFRVSQDNKTLIRNMNAWNNVANVIFLESPAGVGFSYSNTPSDYDLSGDEITADDGFVFLVNWLKRFPEYQYRAFYISGESYAGHYVPELAATILFHNTYHNRTIVNLRGILVGNPYLDANRNVMGKVDFFWTHGVMSDEIYANVTKNCEFDGLGGSTLAEPACIGALDLFDAGQIDGYNIYAPVCIDAPNGTYYPIGYLPGYDPCSDYPTHAYLNDPAVQYALHARTTKWEGCGNLPWKDGPMSMLPTLKFLIESQLPVWIFSGDFDSVCPLPATRFTIQDLGLPVTTPWRPWTSKEEVGGYVQQYAGGFTFLSVRGAGHLVPSFQPERALVMLSAFLKGMLPPYIQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1IS06</accession>
    <name>I1IS06_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100841277</name>
        <name evidence="6" type="ORF">BRADI_4g35680v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Co-chaperone that regulates diverse cellular pathways, such as programmed cell death and stress responses.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578469.1">
        <property type="nucleotide sequence ID" value="XM_003578421.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IS06"/>
    <dbReference type="FunCoup" id="I1IS06">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IS06"/>
    <dbReference type="EnsemblPlants" id="KQJ91116">
        <property type="protein sequence ID" value="KQJ91116"/>
        <property type="gene ID" value="BRADI_4g35680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841277"/>
    <dbReference type="Gramene" id="KQJ91116">
        <property type="protein sequence ID" value="KQJ91116"/>
        <property type="gene ID" value="BRADI_4g35680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841277"/>
    <dbReference type="eggNOG" id="KOG4361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043370_0_1_1"/>
    <dbReference type="OMA" id="PRFNWDL"/>
    <dbReference type="OrthoDB" id="417450at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000298">
        <property type="entry name" value="BAG family molecular chaperone regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF11">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="66"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="5">
        <location>
            <begin position="159"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="241"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00058673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ91116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="36034" checksum="E5533730392AFD64" modified="2012-06-13" version="1">MMRAKAKGTFLDAMKESSPPPPLASSAAAAAKEAVKEDEWEVRPGGMLVQKRSPDSDAPAAPVPTIRIKVKFNGVYHEIYINSQASFGELKKMMSTKTGLHPEDQKVVYKDKERDSKAFLDMSGVKDRSKMVLLEDPAAQAKRLLEQRRTEKAERAAKSISRISLDVDKLATKVSALQSIVGKGGKVAEADVVALTEALMTELVKLDAIAADGDVKAQRRMQEKRVQKYVETLDAIRAKNNTAAAAPKANGNGNAKDRALHLPPRPPPVSQRRQFKQQQPAPANGKAPTANWESFDLLSSMPSTSSATATTTMPSATTAAASPIPRFDWELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1IU44</accession>
    <name>I1IU44_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100833748</name>
        <name evidence="6" type="ORF">BRADI_4g41802v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ROH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578867.1">
        <property type="nucleotide sequence ID" value="XM_003578819.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IU44"/>
    <dbReference type="STRING" id="15368.I1IU44"/>
    <dbReference type="EnsemblPlants" id="PNT65400">
        <property type="protein sequence ID" value="PNT65400"/>
        <property type="gene ID" value="BRADI_4g41802v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833748"/>
    <dbReference type="Gramene" id="PNT65400">
        <property type="protein sequence ID" value="PNT65400"/>
        <property type="gene ID" value="BRADI_4g41802v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833748"/>
    <dbReference type="eggNOG" id="ENOG502QQUT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060027_0_0_1"/>
    <dbReference type="OMA" id="EGFVICQ"/>
    <dbReference type="OrthoDB" id="1878996at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IU44">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008511">
        <property type="entry name" value="ROH1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31509">
        <property type="entry name" value="BPS1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05633">
        <property type="entry name" value="ROH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT65400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="43931" checksum="7086C370AFB62AD5" modified="2012-06-13" version="1">MPVTDYQGSSSSPSPFSFGSLLSLRRDHTAMPSGEEADLELFQRHLAANLVELLPAEGEGGGGGGGGEEILSVAWIRRLLEAFILCQEEFRVVVAQARRRGGALPAAGERLVAEFHERAVKALDVCNAARDGVDQVRRFERLADIAASVLLAPGEIHEGQLRRARKAIADLSVLLVDETGSSASGGVASFLASHRNRSFGRARASPSRAGGSAVGSSSASASHFRSLSWSVSRTWSASRQLQAIGAGLTAPRAHEGGLAAPVYAMGCILHFTAWALVAAVPCPDRSSALLAHHLPAAPARAAFPWAPPLLTLQERLTEEGKRKERRTACGLLKEIQVLEKSTQKLADAIDAAPIPLFGDRETDLREAAAELAAVCAAMRDGLEPLEKQVREVFHRIIRSRVEGLDSSMHNAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZY7</accession>
    <name>I1IZY7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100835067</name>
        <name evidence="3" type="ORF">BRADI_5g16520v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580196.1">
        <property type="nucleotide sequence ID" value="XM_003580148.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZY7"/>
    <dbReference type="STRING" id="15368.I1IZY7"/>
    <dbReference type="EnsemblPlants" id="KQJ83734">
        <property type="protein sequence ID" value="KQJ83734"/>
        <property type="gene ID" value="BRADI_5g16520v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835067"/>
    <dbReference type="Gramene" id="KQJ83734">
        <property type="protein sequence ID" value="KQJ83734"/>
        <property type="gene ID" value="BRADI_5g16520v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835067"/>
    <dbReference type="eggNOG" id="ENOG502QPV8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053056_0_0_1"/>
    <dbReference type="OMA" id="TMPHEKY"/>
    <dbReference type="OrthoDB" id="2538345at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IZY7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34689">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34689:SF1">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="268"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ83734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="341" mass="40563" checksum="B867C0E9D29A3D9F" modified="2012-06-13" version="1">MAKDQNGSAPAPDDGAGKEAARSDSKSLEAEEEESSKGSRDRGHRGKSKRRQEDSESSGEDSGERRKRRRKEKERRRRHRRRSRSESSGSSSESESESSYSGSSAESESESESEEDRRRRRRRRRKEKEEEERRRRRKEKEKRKRKEKERHKKEKDKKKRRKDEKKDLGKKAAVTNSWGKYGIIREVDMWNKRPEFTAWLLEVKQVNLEALSNWEEKQMFKEFMEDHNTATFPSKKYYDLDAYHHRMMEKERKDGLKNAGVVAVRTIFNDEEQRRIELLRERERRKEEEVTALKRSMQTGMAQAMKEQALLREEMMYQYKLGNFEAAAAIQKRLDPDAPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0C5</accession>
    <name>I1J0C5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100834760</name>
        <name evidence="2" type="ORF">BRADI_5g17670v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580280.1">
        <property type="nucleotide sequence ID" value="XM_003580232.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0C5"/>
    <dbReference type="FunCoup" id="I1J0C5">
        <property type="interactions" value="1621"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J0C5"/>
    <dbReference type="EnsemblPlants" id="KQJ83935">
        <property type="protein sequence ID" value="KQJ83935"/>
        <property type="gene ID" value="BRADI_5g17670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834760"/>
    <dbReference type="Gramene" id="KQJ83935">
        <property type="protein sequence ID" value="KQJ83935"/>
        <property type="gene ID" value="BRADI_5g17670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834760"/>
    <dbReference type="eggNOG" id="ENOG502QVYD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_788486_0_0_1"/>
    <dbReference type="OMA" id="WHAVMEN"/>
    <dbReference type="OrthoDB" id="737041at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016606">
        <property type="entry name" value="UCP012943"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33625:SF3">
        <property type="entry name" value="OS04G0550700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33625">
        <property type="entry name" value="OS08G0179900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012943">
        <property type="entry name" value="UCP012943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ83935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="346" mass="37241" checksum="132730464DBCE699" modified="2012-06-13" version="1">MVVHVVYRRRENSWRGSDSSVRFLGAAMSNPPAKHHIRLTSRAAGSPLCSGIALTPASPTGRSGARTGGISLRATSPPPPSSTISIASAASWDSLRLDGEENWEEVVAAGDETLGAEGAADVYGVVLDDPPTDDEVRAAVASIQQVFENPSGVDSDALELQVLALPISGLTSSGMIVNYFAADPDASGMQPVQLVNLGHIDDCAEPAAVSLNSTALLPREHQNVLDAFQLLKEDSSVQKMVMALSTDKAVWNAVMNNEVVQEFKKSFQDAKETDIKGSHSSPPGMMKWVLENTQAKIKEFLEKILQLVNTLFQAQSKDYDLSDDLVRMSFMLSVFVFIVVTIARIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1J1X7</accession>
    <name>I1J1X7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100821566</name>
        <name evidence="4" type="ORF">BRADI_5g22070v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580571.1">
        <property type="nucleotide sequence ID" value="XM_003580523.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J1X7"/>
    <dbReference type="STRING" id="15368.I1J1X7"/>
    <dbReference type="EnsemblPlants" id="KQJ84652">
        <property type="protein sequence ID" value="KQJ84652"/>
        <property type="gene ID" value="BRADI_5g22070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821566"/>
    <dbReference type="Gramene" id="KQJ84652">
        <property type="protein sequence ID" value="KQJ84652"/>
        <property type="gene ID" value="BRADI_5g22070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821566"/>
    <dbReference type="eggNOG" id="KOG4361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043370_1_2_1"/>
    <dbReference type="OMA" id="AKIQTAH"/>
    <dbReference type="OrthoDB" id="417450at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J1X7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
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    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF17">
        <property type="entry name" value="OS04G0619900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="61"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ84652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="266" mass="28929" checksum="9C016795B52CED40" modified="2012-06-13" version="1">MATVMQRSSSDGGSSSGWSEAGAAAAAGEEKAGWEVRPSGMVVQARDREDGAGVPPRPPPPEIRVRVKYGAARHEVAVSPIATFGDLKKVLEARTGLRPAEQLVTYKGRERSNSEYLDACGVKNKSKLVVSEDPVSLERRFIERQRNARIQSANRALGAIALEVDKLADQVKSIEKSVSGGRKVAEVQITTLIELLMRHAVKLESIAADGDSSSSSQKNIQSKRVQKCVETLDVLKVSNARLQTVVVTTKWETFDTPATTQWELFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E5Z3</accession>
    <name>A0A0Q3E5Z3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100828959</name>
        <name evidence="4" type="ORF">BRADI_5g01872v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014750892.1">
        <property type="nucleotide sequence ID" value="XM_014895406.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E5Z3"/>
    <dbReference type="FunCoup" id="A0A0Q3E5Z3">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3E5Z3"/>
    <dbReference type="EnsemblPlants" id="KQJ81628">
        <property type="protein sequence ID" value="KQJ81628"/>
        <property type="gene ID" value="BRADI_5g01872v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828959"/>
    <dbReference type="Gramene" id="KQJ81628">
        <property type="protein sequence ID" value="KQJ81628"/>
        <property type="gene ID" value="BRADI_5g01872v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828959"/>
    <dbReference type="OrthoDB" id="9514740at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3E5Z3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31681">
        <property type="entry name" value="C2H2-LIKE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31681:SF12">
        <property type="entry name" value="C2H2-LIKE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="223"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ81628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="486" mass="50233" checksum="F3F711648DBED298" modified="2016-01-20" version="1">MALSTLLLPTAASAADTSDRRRQHHGKRKKKPPPSPSPRTTPPPAARTSTSGSRHRAAASPSKIKSPAAAMDASSKLGAAGKSHQRHHSKPSKKAAASSSSSSSSSSWEQLKSLLSCRSATAAARVHDPAASSSSARLRAGGACGASLCSIRDVLIDGGASASSVASSAAPLNRSSRRTHRSVTPSSTGGHPSSLRGLSGCYECRAINVEPVSRRYPRPRELCPCSQCGEVFTKAESLEHHLAIRHAVSELGPEDSGRNIVEIIFKSSWRQQRQSPVGMGAAMCQIERILKVHNPARTVARFESYRDAVRARCRAATAATTRAAADGNELLRFHPARLSCALGLAGSSSLCSSTTAADDTDPQSASATPSGNGECCGVCAAIRHGFAPWAGEHALGVRTTASSGRAHQGAGAAGDGAGSARAMLVCRVIAGQVRKAGGEEEGLFDSVAGEEDGGGAYGSMEELFVANPRAILPCFVVIYRVAAPSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E8I0</accession>
    <name>A0A0Q3E8I0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100828851</name>
        <name evidence="3" type="ORF">BRADI_5g10200v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ82644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_5g10200v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ82644"/>
        <property type="gene ID" value="BRADI_5g10200v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="903824at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="entry name" value="Lea14-like_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31852">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
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            <begin position="16"/>
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        </location>
    </feature>
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        <location>
            <begin position="86"/>
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        </location>
    </feature>
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            <end position="116"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="442" mass="47509" checksum="E806EB34243A1297" modified="2016-01-20" version="1">MLPVVQLARIRVPQTIPGAGSHRSALLPSPNYPSRDASRSSAHATPLHDESKNRRGRALRPLHTSTRPRATRDSHATHTNAASDQRRRRPRISPRFRWRPEPHARPEAMHHAKTDSEVTSSMAPSSPPARAAYYVQSPSHDDGEHKTTAASSFHSSPAASPPRSLGNHSRESSSSRFSGKVLPSSAATGSSRRTGPGGRGGEAGAGAGGRRSSPWMKEAAIEEEGLLMEDDSEDAAHGGGFAALPKKVRYGLGFVGAFFALFFFFALILWGASRNQKPVVSLNSVTFHNFVIQAGTDASLVPTELSTINATVKLTFRNTGTFFGVHVTAQPVTLYYSQLLMASGNMKYFYQPRKSQRNVAVTVVGSKVPLYGGGAGLSSTPGPKGAPPPPVPLQLTLRIRARALVLGKLVKPKFYSDFQCSLRLDVAKLGKPVSLKKSCTHV</sequence>
</entry>
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        </submittedName>
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    </gene>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblPlants" id="KQJ86182">
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        <property type="gene ID" value="BRADI_4g03800v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_4g03800v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830779"/>
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    </dbReference>
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    </evidence>
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    <sequence length="721" mass="79728" checksum="130B0FACE6DCA166" modified="2016-01-20" version="1">MFLLPFSDQPKPKPRKSPIPNPQCLLSNDALFLLFPPASASAPFLPAAALSPRRRRASHRLPISWIASLRCGDDEVHVLLLKNWCEVCPMDKQKNHKNAEMGKDLFRRTILSQIAGGVSFEEENAPCNTPRNSVHSQLGGSFVASTSESTGAESVSPGEYVRDPGSILSLQPWIFKRSGAQNSEERIVVSGSKVGRGKNLVDGFRDGSTVDVSARSPALGSGPGRGRGALRSRRSHRHFIKPLVPMEDSYIPQLYNGNFEFEECTFAPVPSPASARPFIVTDGRRIISKSSYGPVPVPFDIGLNKEECRNGSNVLESVVGIAPLPELNRDSTVSHDVRLSLSSSKRGSEPSKSAGLLERMLMFSSGVSIGVISSSLSNKKDLDSLNGTLKRMENLVQDLQDELEMKEGLTVKELPYESEHDDGNIKGYTVDSEPMSKIEAELEAELARLELNITSNHLEEEQPFDFSEVDQEFVGDIVQGELKIDMIRRDIADYSSESDHGRDSRQSSPDCTRDANYPVSPRDLSLRLHKVIQHRLEERIKELETALAQSQKQAQLQMMARERVFSEQTSSNSVSGSSSNQDSPLFIQETSSSAEPFCLNLAGDALEAYDEAYEEFMRIADSPCTTSTNGKPQVNEYYSVDRSLVWGLEDDSATELKEVPTWEQAVKSGDPNRAQESDGEDDCSDEDDHDSKMLIQQIIERTKQGSPVLIHAQKMLFSVDE</sequence>
</entry>
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    <accession>A0A0Q3EFW7</accession>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
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    </dbReference>
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        <location>
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        </location>
    </feature>
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            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ86583.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="243" mass="26611" checksum="5012936431432075" modified="2018-03-28" version="2" fragment="single">LLPQPPAPGNPSRHRRRLVLLRPIPHQHPAPAAASFMANADPNSAAFLVDLLAEESVTEPPPLQEGAAEPETEFVPVQGANVEVIMVGGLPMEVEPMSPDWPFPQPASLQEPPRALGSPSHKRRLSDDLDSEPEMAGFDDAMGLPYGTGIHNEMPSLPPDASSTIYAEGLPSNITGRELAHIFRPFNGFREVRLVNDNSHKIAFVDYATPAEAFSAMRALQGYWLDIDDQDSRLHLQLSRDQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EIQ9</accession>
    <name>A0A0Q3EIQ9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100834861</name>
        <name evidence="9" type="ORF">BRADI_4g04370v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane magnesium transporter (TC 1.A.67) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579044.1">
        <property type="nucleotide sequence ID" value="XM_003578996.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EIQ9"/>
    <dbReference type="STRING" id="15368.A0A0Q3EIQ9"/>
    <dbReference type="EnsemblPlants" id="KQJ86268">
        <property type="protein sequence ID" value="KQJ86268"/>
        <property type="gene ID" value="BRADI_4g04370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834861"/>
    <dbReference type="Gramene" id="KQJ86268">
        <property type="protein sequence ID" value="KQJ86268"/>
        <property type="gene ID" value="BRADI_4g04370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834861"/>
    <dbReference type="OrthoDB" id="44756at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EIQ9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018937">
        <property type="entry name" value="MMgT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181:SF7">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10270">
        <property type="entry name" value="MMgT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ86268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="106" mass="11553" checksum="C07F5A66F45043DB" modified="2016-01-20" version="1">MGIGYVVGVLGGALLAHAAYATIQYRAVLKITEEEFSRPPVDVMMQLLLGLALCMWAGVSVPAKFVSVLPHSEENRIVSLPANMDFMIFNHRGRALPSDADLKLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FX81</accession>
    <name>A0A0Q3FX81_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100824371</name>
        <name evidence="10" type="ORF">BRADI_2g11430v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565694.2">
        <property type="nucleotide sequence ID" value="XM_003565646.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FX81"/>
    <dbReference type="STRING" id="15368.A0A0Q3FX81"/>
    <dbReference type="EnsemblPlants" id="KQK04064">
        <property type="protein sequence ID" value="KQK04064"/>
        <property type="gene ID" value="BRADI_2g11430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824371"/>
    <dbReference type="Gramene" id="KQK04064">
        <property type="protein sequence ID" value="KQK04064"/>
        <property type="gene ID" value="BRADI_2g11430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824371"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FX81">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000011">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000107">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044676">
        <property type="entry name" value="EOBI/EOBII-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675:SF6">
        <property type="entry name" value="OS01G0298400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="19"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="19"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="72"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="76"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK04064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="295" mass="32792" checksum="3CA8858E024847D0" modified="2016-01-20" version="1">MSTGMDAMWTAAGSEDSAAAELRRGPWTVEEDVLLAGYIKAHGEGRWNELARLAGLRRTGKSCRLRWLNYLRPGVRRGDFSPQEQLLILELHFRWGNRWSRIAQELPGRTDNEVKNYWRTRVQKHAKQLRCDVDSRQFQDVMRHLWMPRLAERIHAAARASATTSSASSTTSSEHAAATLHPMYRPNNSLLGPMSDMPSPDTTTTWSTATARPSSGALFPSNQVSMAAAAPGCSADWSAGSASARSDDMFDGSWSELLARACDDGADSLVFPDFGLGESRDNSWSLDDIWSQHQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G176</accession>
    <name>A0A0Q3G176_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin B-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100835402</name>
        <name evidence="7" type="ORF">BRADI_2g18740v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Interacts with CIPK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231116.1">
        <property type="nucleotide sequence ID" value="XM_010232814.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G176"/>
    <dbReference type="STRING" id="15368.A0A0Q3G176"/>
    <dbReference type="EnsemblPlants" id="KQK05215">
        <property type="protein sequence ID" value="KQK05215"/>
        <property type="gene ID" value="BRADI_2g18740v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK05216">
        <property type="protein sequence ID" value="KQK05216"/>
        <property type="gene ID" value="BRADI_2g18740v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT70823">
        <property type="protein sequence ID" value="PNT70823"/>
        <property type="gene ID" value="BRADI_2g18740v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT70825">
        <property type="protein sequence ID" value="PNT70825"/>
        <property type="gene ID" value="BRADI_2g18740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835402"/>
    <dbReference type="Gramene" id="KQK05215">
        <property type="protein sequence ID" value="KQK05215"/>
        <property type="gene ID" value="BRADI_2g18740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05216">
        <property type="protein sequence ID" value="KQK05216"/>
        <property type="gene ID" value="BRADI_2g18740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70823">
        <property type="protein sequence ID" value="PNT70823"/>
        <property type="gene ID" value="BRADI_2g18740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70825">
        <property type="protein sequence ID" value="PNT70825"/>
        <property type="gene ID" value="BRADI_2g18740v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3G176">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000073">
        <property type="entry name" value="calcineurin B-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045198">
        <property type="entry name" value="CNBL1-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056">
        <property type="entry name" value="CALCINEURIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056:SF110">
        <property type="entry name" value="CALMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="158"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="195"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="239"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK05215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="307" mass="34928" checksum="B1B6825284951C12" modified="2016-01-20" version="1">MGQIHFDLVLSERKDGASRLTSRADRELDPSRAQQRAGEEPPAAGFRFPSLLFPLTSRSESNRRSRIEELFFLGVLDWLPRRRRETEKMGCGASSALKRHKRAPGYEEPAVLASETTFTVNEVEALYELYKKLSFSIIKDGLIHKEEFQLALFRSSKGANLFADRVFDLFDLKRNGVIEFGEFVRSLSIFHPKTPDSEKTAFAFKLYDLRGTGYIEKEELKEMVLALLDESDLRLSDSAVEAIVDTTFNQADSNGDGRIDPKEWEEFVKKNPTSLRNMSLPYLQDITTAFPSFVMHSEAEDYSGTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GBR8</accession>
    <name>A0A0Q3GBR8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g38731v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GBR8"/>
    <dbReference type="EnsemblPlants" id="KQK07973">
        <property type="protein sequence ID" value="KQK07973"/>
        <property type="gene ID" value="BRADI_2g38731v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07973">
        <property type="protein sequence ID" value="KQK07973"/>
        <property type="gene ID" value="BRADI_2g38731v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GBR8"/>
    <dbReference type="OrthoDB" id="695058at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK07973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="17584" checksum="1F85F58930C17245" modified="2016-01-20" version="1">MQTEQLSDFVALWECIQGVHLSEENDSISWRWTPSGSYTTASAYRIQFAGSLPHFESRKIWKVWRHIQTWSLPSPSTTDINAHWECLIHGLPKKETRTLSERLITSWWGIWKELNRRIFRSSALPPLEVAYLVWEEVQSRSLASSIDPGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GSC6</accession>
    <name>A0A0Q3GSC6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100823517</name>
        <name evidence="4" type="ORF">BRADI_1g12200v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559565.1">
        <property type="nucleotide sequence ID" value="XM_003559517.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GSC6"/>
    <dbReference type="FunCoup" id="A0A0Q3GSC6">
        <property type="interactions" value="2165"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GSC6"/>
    <dbReference type="EnsemblPlants" id="KQK13744">
        <property type="protein sequence ID" value="KQK13744"/>
        <property type="gene ID" value="BRADI_1g12200v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823517"/>
    <dbReference type="Gramene" id="KQK13744">
        <property type="protein sequence ID" value="KQK13744"/>
        <property type="gene ID" value="BRADI_1g12200v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823517"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GSC6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001066">
        <property type="term" value="F:amylopectin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000014">
        <property type="term" value="P:regulation of endosperm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019252">
        <property type="term" value="P:starch biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47434:SF1">
        <property type="entry name" value="PROTEIN PTST HOMOLOG 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47434">
        <property type="entry name" value="PROTEIN PTST HOMOLOG 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="3">
        <location>
            <begin position="455"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="403"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK13744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="532" mass="57998" checksum="20F850D6C5FB71AB" modified="2016-01-20" version="1">MPPFLTCSFLSLPFPPLTLPFLPAPPPRRRRVSAAPLAAAAYRPPQRQPYYRRRPAPPPPQPQTRPSNAGPPPPRGAMGQEDLEAAIYDFMRSSDKPGAFPTRAELLAAGRADLARAVESSGGWLSLGWSSGDSVSPCVTASASSLDGGAGVHPDYPPEAAGTSDLAPGAEGASWREPEASPSGRPPETEGTEEVVSGAGLEGMLTRLQRERERARPPPRSNNRGGGGGGGDNGVLMNHNGAPSHTATGGMRTRRVFDNGNIHSSQLQNGILADNKSSRNSTNDAWRTWSLDRGGFSDFQVAEIHPSGSKKVLKHDDLDIALVQDDIPGPSNGVAISDYPSDGVDSERDEIHARLQNLELDLTDALKTLRSRFDSVLTDLSNGDGTNVVNGLSDDWEFEETKVMHAQEELRSIRAKIAVLEGKMALEIIERNKIIEEKQRRLDEVEKALSELRTVYLVWPNPASEVLLTGSFDGWTSQRRMEKSERGIFSLNLRLYPGRYEIKFIVDGVWRNDPLRPTLNNHGHENNLLIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GSY2</accession>
    <name>A0A0Q3GSY2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_5g18850v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GSY2"/>
    <dbReference type="FunCoup" id="A0A0Q3GSY2">
        <property type="interactions" value="596"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GSY2"/>
    <dbReference type="EnsemblPlants" id="KQJ84122">
        <property type="protein sequence ID" value="KQJ84122"/>
        <property type="gene ID" value="BRADI_5g18850v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ84122">
        <property type="protein sequence ID" value="KQJ84122"/>
        <property type="gene ID" value="BRADI_5g18850v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GSY2"/>
    <dbReference type="OrthoDB" id="1932364at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GSY2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045277">
        <property type="entry name" value="DRE1A-I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31839:SF85">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31839">
        <property type="entry name" value="DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="13"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="167"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ84122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="197" mass="20439" checksum="5FA2BF719A5FB987" modified="2016-01-20" version="1">MSEHGGSSGKHPFYRGIRSRCGKWVSEIREPRKARRIWLGTFPTPEMAAVAYDVAARALRGPDAALNFPAIAASRPAPASTSADDIRAAAAAAAASLAGGGGIAPPGGAAGSAVQQQQQFGGGSGTAAGSEEAASIGANYYNQQYFLDEEALFETPQFLRSMAAGMMMSPPRLSPDSSDESPDPSEAGESLWSYRDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H160</accession>
    <name>A0A0Q3H160_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100827326</name>
        <name evidence="18" type="ORF">BRADI_1g30160v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16712.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560328.3">
        <property type="nucleotide sequence ID" value="XM_003560280.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H160"/>
    <dbReference type="STRING" id="15368.A0A0Q3H160"/>
    <dbReference type="EnsemblPlants" id="KQK16712">
        <property type="protein sequence ID" value="KQK16712"/>
        <property type="gene ID" value="BRADI_1g30160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827326"/>
    <dbReference type="Gramene" id="KQK16712">
        <property type="protein sequence ID" value="KQK16712"/>
        <property type="gene ID" value="BRADI_1g30160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827326"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H160">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010080">
        <property type="term" value="P:regulation of floral meristem growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048833">
        <property type="term" value="P:specification of floral organ number"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000565">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase protein FLORAL ORGAN NUMBER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000201">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000219">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000108">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000560">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF44">
        <property type="entry name" value="RECEPTOR PROTEIN KINASE CLAVATA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="761"/>
            <end position="1050"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK16712.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1068" mass="112650" checksum="907318CFB9365650" modified="2018-03-28" version="2">MIPIPPPIDSFPPYSDSLLHHHPPFIPASLSMHSPSPFPAFAAGNRNRKFAMPRPIILFFFFVFLGPHAAASPDRDIYALAKLKAALVPNPSSSPSTALADWDPAAVSPSHCSFSGVTCDPATSRVVSINITSVPLHTGGQLPPELALLDALTNLTIAACSLPGLLPLHPNIPSLPNLRHLNLSNNNLIGPFFLPDSVTTTPYFPSLELLDCYNNNLSGPLPPFGAPHSATLRYLQLGGNYFSGPIPPSYGHLASLRYLGLNGNALSGRVPPELARLAKLEDLYLGYFNQYDGGVPPEFGELASLVRLDMSSCNLTGPVPPELGKLSKLQTLFLLWNRLQGAIPPELGELASLQSLDLSVNELAGEIPVSLGKLSNLKLLNLFRNHLRGDIPAFVAELPGLEVLQLWENNLTGSLPPGLGKKGPLKTLDVTTNHLTGLVPPDLCAGNKLETLVLMDNGFFGPIPASLGACKTLVRVRLSRNFLSGAVPAGLFDLPDANMLELTDNLLSGELPDVIGGGKIGMLLLGNNGIGGRIPAAIGNLPALQTLSLESNNFSGELPTEIGRLRNLSRLNVSGNSLTGAIPEEITSCASLAAVDVSRNRLSGEIPQSVTSLKILCTLNLSRNAIGGSIPPAMANMTSLTTLDVSYNRLSGPVPSQGQFLVFNESSFLGNPGLCNAGADNDDCSSSSSSSPAAGGGLRHWDSKKTLACLVAVFLALAAAFIGAKKACEAWREAARRRSGAWKMTVFQKLDFSAEDVVECLKEDNIIGKGGAGIVYHGAIVSSSTGSVGAELAIKRLVGRGAGGDRGFSAEVATLGRIRHRNIVRLLGFVSNREANLLLYEYMPNGSLGEMLHGGKGGHLGWEARARVALEAARGLCYLHHDCAPRIIHRDVKSNNILLDSAFEAHVADFGLAKFLGGAGAGGGNGASECMSAIAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLELVTGRRPVGGFGEGVDIVHWVHKVTAELPDTAAAVLAIADRRLSPEPVALVAGLYDVAMACVEEASTARPTMREVVQMLSQPVIAAAAAGDAAQPDDLLVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H1T5</accession>
    <name>A0A0Q3H1T5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TTF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g31303v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16895.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H1T5"/>
    <dbReference type="STRING" id="15368.A0A0Q3H1T5"/>
    <dbReference type="EnsemblPlants" id="KQK16895">
        <property type="protein sequence ID" value="KQK16895"/>
        <property type="gene ID" value="BRADI_1g31303v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16895">
        <property type="protein sequence ID" value="KQK16895"/>
        <property type="gene ID" value="BRADI_1g31303v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H1T5"/>
    <dbReference type="OrthoDB" id="1705400at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055298">
        <property type="entry name" value="AtLOH3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006580">
        <property type="entry name" value="Znf_TTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 2-RELATED ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697:SF230">
        <property type="entry name" value="ZINC FINGER, MYM DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
        <property type="entry name" value="ZnF_TTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTF-type" evidence="2">
        <location>
            <begin position="135"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK16895.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="813" mass="91960" checksum="1BE740E7717676D2" modified="2018-03-28" version="2">MKKRSVSSGDRSTGDLRRYLTKKSSAPPTDDGADETERTIPNPPRNESDPIPPISESTNPATNVSTNSTVDESTHRPKRLRLEFDPNHIIPDPALRIPIKDYANEIRDEVRRAYLLKGRTEANGHVFEQTLDGKVWRSFQRSWLGKYDWLEYSVKENAAFCFYCFLFKKTAQPNTFGNDVFSRDGYSRWKTATAAFKKHVGGPNSFHNIARGKCEDFINQRSSVSSKIHTYSKESEIAYTIRLTASLDCARYLIAQGEAFRGHDESSTSINKGNFKELLEWYKDKNSEVKEAFEKGPGNALMVCSDIQKQLAKCCAQEVTSVIKSEIGDRNFAVLIDEARDSSIKEQMAVIVRFVNNQGKVMERFLAMKHISDCTSAALKEALLHLLALHDLPISRLRGQGYDGASNMRGEFNGLQKLIRDDSPHAFYVHCFAHQLQLVVVTVAKCSKCISDFFNHVPLIVTQVSASCKRKDTLIAKHQDVLLEKLENGKISSGKGLHQESGLARPGDTRWGSHLKTLLRIYQMWESVVDVLTIVSTDAREHSSVGGAGGLVKKMECYEFVFILNFLIGLLTITNDLSQALQRKDQDIVEAMGLISDVKTRLQDVRDNGWEPLLDQVKSFCEEYGINVPNMDDLTVPMGESVRSKNKVTLRHYYKVDIFNVAIDATMTEINHRFNEVSSELLVCMSCLNPSNSFAMFNVDKLVRLAEIYEADFTISNRLMLRSQLQIFILNIRRSEKFHGCSDISKLSQLMVETKKDKTFPLVYRLIELTLILPVATASVERIFSAMSIIKTDLRNKLSDGWLNDRAGDIQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I777</accession>
    <name>A0A0Q3I777_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_3g22538v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I777"/>
    <dbReference type="STRING" id="15368.A0A0Q3I777"/>
    <dbReference type="EnsemblPlants" id="KQJ96339">
        <property type="protein sequence ID" value="KQJ96339"/>
        <property type="gene ID" value="BRADI_3g22538v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96339">
        <property type="protein sequence ID" value="KQJ96339"/>
        <property type="gene ID" value="BRADI_3g22538v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3I777"/>
    <dbReference type="OrthoDB" id="682279at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF961">
        <property type="entry name" value="DISEASE RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="22"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="163"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="310"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="450"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ96339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="741" mass="85001" checksum="E1EBED0EFF6276EA" modified="2016-01-20" version="1">MIWFPVCIQALAGLHDMAEGIVVSLILKLGDALSSKVFELVGSLFRLEGYALECLFCEIWQVKGELESIQAFLRAAESFRDTDETTAAFVRQIRILCFHIEDVIDEFTYELGKDGGGMLLLKALRRMRQIKIWHRLAARLRDIKEDDPVGIKEQQDFLMTWVEDEEPRNSIASVWGMGGIGKTTLANHVYSVVKDGFHTCAWITVAQSSEADDLLRKIVQEFHKNDRTKEFPKDVDLTDFRSLVKAIRCYLEDKRYILVLDDVWDMNVWFDIKYAFLDGNGRILFTSRIYQVSLEDLPHSIRSCFLYCSMFPEKYVMQRKSLVRLWVAEGFAEESEHKTLEEVAEDYLTELIHRCLLLVFKRNDSGCVYEVQMHDIVRVLARSKAREENFCRVFNPLRTYAIRESRRISTEAGDIQLLTQNALHLRSLLVFQSSFSFVSLRSLLMTNKLLSVLNLQDSSIEKLPAEVFELFNLRLKNLLVLDAWKCKIMKLPPQITKLHKLTHLIVTSKPGLPTLQFVPSVGVPAPFGICSLTSLQTLLQVEASAEMIRLIGALVELRTLRISKVQGLHCEDLFRAIGSMVHLTRLGIQAEDNQEMLWLGKFEPPPLLEKLFLLGALSKESSPDFFSSRSKLKNLKFLRLVGSRLDKDTFSCLKGLEHLVKLQLYDAYAGDSMYFPAASFPKLRLLLCPQLKMLPHGIEYVSTLEELTLDTAQELVDRVRQKKESKISHVQRVMVELQNCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I9J9</accession>
    <name>A0A0Q3I9J9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100823971</name>
        <name evidence="14" type="ORF">BRADI_2g01746v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02483.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565277.1">
        <property type="nucleotide sequence ID" value="XM_003565229.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315640.1">
        <property type="nucleotide sequence ID" value="XM_024459872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I9J9"/>
    <dbReference type="STRING" id="15368.A0A0Q3I9J9"/>
    <dbReference type="EnsemblPlants" id="KQK02483">
        <property type="protein sequence ID" value="KQK02483"/>
        <property type="gene ID" value="BRADI_2g01746v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823971"/>
    <dbReference type="Gramene" id="KQK02483">
        <property type="protein sequence ID" value="KQK02483"/>
        <property type="gene ID" value="BRADI_2g01746v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823971"/>
    <dbReference type="OrthoDB" id="4788989at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16571">
        <property type="entry name" value="RING-HC_SIAHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF103">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="35"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="SIAH-type" evidence="13">
        <location>
            <begin position="89"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK02483.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="312" mass="33995" checksum="C5B0795587AE4B61" modified="2018-03-28" version="2">MDGSYSRKRTAEAQQEGEHSAKRLNVTVGMETLDCPICYNPLEPPIFQCSVGHFICSSCRGKQLDKKCPSCCIKTSFKRYFGMEHVVQSATVPCSNAKYGCAVKVAYYHKEEHEKACPNTPCFCPESGCGFAGTTMALLDHLTNQHKCPSTTLPDSAFSADSGTVNLCLQPGLHLLRCGRSVTSYFFLFSMASEPFGHAISVVCVQPNVTKPKSCNMKYECSTIGYCEISSCQIRSSSLSDGLPTGYDLILPKGKVSDDRNGIRLRTTIIQPLSFSLVTSCLQGKGLTPTLQEQPFAARGIPDDSDSDPDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IF29</accession>
    <name>A0A0Q3IF29_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100824305</name>
        <name evidence="11" type="ORF">BRADI_3g41910v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572474.3">
        <property type="nucleotide sequence ID" value="XM_003572426.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235363.2">
        <property type="nucleotide sequence ID" value="XM_010237061.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IF29"/>
    <dbReference type="EnsemblPlants" id="KQJ99235">
        <property type="protein sequence ID" value="KQJ99235"/>
        <property type="gene ID" value="BRADI_3g41910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824305"/>
    <dbReference type="Gramene" id="KQJ99235">
        <property type="protein sequence ID" value="KQJ99235"/>
        <property type="gene ID" value="BRADI_3g41910v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824305"/>
    <dbReference type="OrthoDB" id="774413at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IF29">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000003">
        <property type="entry name" value="Probable disease resistance protein At5g66910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF64">
        <property type="entry name" value="OS12G0206100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="9"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="170"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="427"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="10">
        <location>
            <begin position="680"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ99235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1201" mass="137515" checksum="F0C32FFF2C5A1ABF" modified="2016-01-20" version="1">MAESLLFAVVRAAARKAADVLVQNMTRMCGIEDDRCKLERQLLAVQCKLADAELRSETNQYVKRWMKDFRTVAYEAADVLDDFQYEALRREAQIGESRTRKVLDHFTPHCALLFRLTMSRKLHNVLEKINQLVEEMNKFGLVERAEPPQFLYRQTHSGLDDSAGIFGRDDDKELVVKLLLDQRDQLKVHVLPIFGMGGLGKTTLAKMVYNDGRVQQHFQLNMWHCVSENFEAIDLVKSVIELATQKNCDLPYTIELLRGRLQEVIGQKRFLLVLDDVWNEEKRKWEDDLKPLLCSVGGPGSVILVTCRSRQVASIMTTLRPHELECLSEDDSWELFSEKAFSNGVEEQAELATIGRRIVKKCRGLPLALKRIGGLMSSKQQVQQWEAIAERNIGDNSRGKDEIISILKLSYRHLSPEMKQCFAFCSVFYKDCEMEKDMLIQLWIANGFIQEEGTMDLPQKGEFIFHYLVWRSFLQDVKLKEVHFSRKVICCKMHDLMHDLAKDVTDECATMEDLIQEIQQRASIKDARHMQIITPGQWEQFNGLFKGTRYLHTLLGSFATHKNLKELRLMSVRALHSYVPSIIHYQVINAKHLRYLDLSESGIGRLPDSICVLYNLQSLRLNGCWKLRQLPEYMSNMRKLIHLYLFGCDGLERMPPKLSLLNNLHTLTTFVVDSGDGHGIEELKDLQHLANRLELYNLRKVKSGENAMEANLHEKQNLRELLLYWGRCTYDQSEHEACNEEQVLDCLAPHSKLQILNVAGYNGLKVSQWMRDPQMFQCLRKLKISNCPRCKDLPVVWLSVSLEYMCLESMGGLTTLGKNIGVEEDGYNTHLQIFPRLKGMALNDLPSLDRWMENSAGEPINYIMFPMLEVLSISCCPKIASVPESPVLKNLRIGGLCSPPISSLTHLTTLSELAYFGNDIVSKSMPLGSWPSLKKLQVGSLANMMMVPPEDWHSQSQRRALETLQSLSLYGPYCFVAPSRLSRSHLGYWECFAFVEELTIHSSNELVLWPMEELRILSRLRSLCIFFCANLEGKGSLSEESLPLPQLERLDIRNCHSLVKIPNLPTSLEQLKIFDCENLVELPSNLEDLAKLRVLDVNTCRCLKALPDGMDGLTSLEQLRIGYCPGINEFPQGLLQRLPLLKSLCISTCPELQRRWREGGEYFHLLSSIPEKSIRYTETESSSKNFLRRLIPSCQLPESAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IFS4</accession>
    <name>A0A0Q3IFS4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g15805v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IFS4"/>
    <dbReference type="FunCoup" id="A0A0Q3IFS4">
        <property type="interactions" value="697"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK04763">
        <property type="protein sequence ID" value="KQK04763"/>
        <property type="gene ID" value="BRADI_2g15805v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04763">
        <property type="protein sequence ID" value="KQK04763"/>
        <property type="gene ID" value="BRADI_2g15805v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IFS4"/>
    <dbReference type="OrthoDB" id="696382at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF5">
        <property type="entry name" value="OS09G0314100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK04763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="469" mass="52143" checksum="515F7A07B1C51ADF" modified="2016-01-20" version="1">MEISPAQRRNNSQGTDSVGNGDLGANSGGTDSVRTKRAKVAAVETRGRHKRSRTQLRNLYEDGKQFKYRDYTRGDYKYTTQLGCILKRQYHGALTDGNGELYPATTWADYYEKTNDQGETCAHKRLFTVADEHKREAARVLENYCKKRVWNIMYQVRVDAVKLHYENQGEILDDTLACRRELNENEYPNARVEWCKEDVWPLLAAYWDSDEFKEKRSKAHDSRLSNEDLAWCLQEVVHGPEKATILNTYAVMKSGAKNMNNNGSSGVIPSQKAQKRMRDYQEGVEAAHPDDPEQTEVDGLVLYTKGGGLPHGRLLIGDGTVRKVNVIAAAKGKKSRPSTSDSYQHLSEENHQLRRANEGLTQHNVHLTQQQEVDHELIMGAPGSSHAGSQSIHNDGMDGAGTSTNGNNNVVNVEINDTTTIGLGNNRGDILAEENGSDDDSEALYANSDIDAGHDNDACAGREADRDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IM29</accession>
    <name>A0A0Q3IM29_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g55110v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IM29"/>
    <dbReference type="FunCoup" id="A0A0Q3IM29">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3IM29"/>
    <dbReference type="EnsemblPlants" id="KQK01316">
        <property type="protein sequence ID" value="KQK01316"/>
        <property type="gene ID" value="BRADI_3g55110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01316">
        <property type="protein sequence ID" value="KQK01316"/>
        <property type="gene ID" value="BRADI_3g55110v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IM29"/>
    <dbReference type="OrthoDB" id="653596at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IM29">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK01316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="20026" checksum="24855F16CFCA6658" modified="2016-01-20" version="1">MLQKPDRFDSSGSPTPNPESNATQSPLICSPSCSAAAPPRAGATRNRRRSACVAMAAPPTGPIACPVYPWPSDGAAQGRKKLFMQSDCAACHSALPYAGLREATASADVRAQEANIVIAAAAEVAQPQPRHLHGGPPPDLAALVTRIQGSTGGEVAKTTMMAGGTAVCEELKKKITAPLSPVWLHYTRTFQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ITF5</accession>
    <name>A0A0Q3ITF5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_4g25601v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89408.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ITF5"/>
    <dbReference type="FunCoup" id="A0A0Q3ITF5">
        <property type="interactions" value="466"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3ITF5"/>
    <dbReference type="EnsemblPlants" id="KQJ89408">
        <property type="protein sequence ID" value="KQJ89408"/>
        <property type="gene ID" value="BRADI_4g25601v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89408">
        <property type="protein sequence ID" value="KQJ89408"/>
        <property type="gene ID" value="BRADI_4g25601v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3ITF5"/>
    <dbReference type="OrthoDB" id="687302at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033239597" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="44"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ89408.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="255" mass="26409" checksum="B78C629653ABC053" modified="2018-03-28" version="2">MATKLCLALLLVVMAVAATPAAATRRRPESRVLWYPGIRPRGGSSPSVFPGWPRRPSSPPASSRFSPPSPRPSPPASPSPVVPTPPSSTPPPCVAAARPLPGFPGVSSSPSTSSSSAPADCVTPLAGLMTCGPFLTGSAQDTPAPGSECCSGLGMFLNSTAAAEDRSLRCLCPVILGDVNRMLPRPVDPVRMMYLPISCGVVLPPQVLFICFTGQPTPPVVSRIPDSWRKTFFSSSSLSSSSSSGNQSTLTPPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IY63</accession>
    <name>A0A0Q3IY63_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor MYBS1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Myb-related protein S1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100830302</name>
        <name evidence="13" type="ORF">BRADI_2g55217v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564630.1">
        <property type="nucleotide sequence ID" value="XM_003564582.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IY63"/>
    <dbReference type="STRING" id="15368.A0A0Q3IY63"/>
    <dbReference type="EnsemblPlants" id="KQK10627">
        <property type="protein sequence ID" value="KQK10627"/>
        <property type="gene ID" value="BRADI_2g55217v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830302"/>
    <dbReference type="Gramene" id="KQK10627">
        <property type="protein sequence ID" value="KQK10627"/>
        <property type="gene ID" value="BRADI_2g55217v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830302"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IY63">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009739">
        <property type="term" value="P:response to gibberellin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009744">
        <property type="term" value="P:response to sucrose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="12">
        <location>
            <begin position="143"/>
            <end position="199"/>
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        <location>
            <begin position="143"/>
            <end position="195"/>
        </location>
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        <location>
            <begin position="219"/>
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        </location>
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        <location>
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            <end position="242"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51293"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="KQK10627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="308" mass="33360" checksum="77B321EC669C9E72" modified="2016-01-20" version="1">MMAEAWTDVLLPPASFFSAHPCNSWFAQADRRGASGGGGGAWTAEENKVFEEALAAIDLGAPDGWEMVALMLPEKTVAEVVSHFRALENDVGFIEAGLVPFPRYDHDHDASPPSSAGFTLDWDDGGGFRGRGYFLRRGGRADKERKKGVAWTEEEHRLFLKGLKKYGRGDWRNISRSYVTSRTPTQVASHAQKYFNRLSSSGGGGKDGKRRASIHDITIVNLPDDDHGHGSTSPSALTTASDGQFGAHVDAKPSSLSPPSLGRHRHFLPPGTIISSHHPYGSVKLEQQSSFMAGSTGLDLLQMQCGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JT48</accession>
    <name>A0A0Q3JT48_BRADI</name>
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        <recommendedName>
            <fullName evidence="7">GTD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_1g56922v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3JT48"/>
    <dbReference type="EnsemblPlants" id="KQK20819">
        <property type="protein sequence ID" value="KQK20819"/>
        <property type="gene ID" value="BRADI_1g56922v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20819">
        <property type="protein sequence ID" value="KQK20819"/>
        <property type="gene ID" value="BRADI_1g56922v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JT48"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080115">
        <property type="term" value="F:myosin XI tail binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="GTD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31422">
        <property type="entry name" value="BNAANNG28530D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31422:SF21">
        <property type="entry name" value="OS07G0162200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zein-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GTD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GTD-binding" evidence="7">
        <location>
            <begin position="38"/>
            <end position="132"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
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            <end position="225"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK20819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="270" mass="30036" checksum="3E42A60A26CEC65C" modified="2016-01-20" version="1">MDPMATTWSRSVRRRAPASEQDEAERRELIQKAEELEEAVEALRAERDAAAEEEAALRAELEAERGAAESAASEAMRMIERLQRETAALLLEARQLRRLAESRAGRDRELESRLASLSALARGYLSLLRAHGIDPDAEEDDQQERPPVAGEEFEDTGRVVAMAAAAEEEREQDRVAVDAAAEDLCARLEALEADSTAERREVAALRAERARVVLAREMARRLRQEAAAVAERPPDAAVRAAHKPRFCVLAVCKVLCLLRYRLRAAVEFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LL30</accession>
    <name>A0A0Q3LL30_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100838403</name>
        <name evidence="9" type="ORF">BRADI_3g03020v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SFH family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574138.1">
        <property type="nucleotide sequence ID" value="XM_003574090.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LL30"/>
    <dbReference type="EnsemblPlants" id="KQJ93165">
        <property type="protein sequence ID" value="KQJ93165"/>
        <property type="gene ID" value="BRADI_3g03020v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838403"/>
    <dbReference type="Gramene" id="KQJ93165">
        <property type="protein sequence ID" value="KQJ93165"/>
        <property type="gene ID" value="BRADI_3g03020v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838403"/>
    <dbReference type="OrthoDB" id="1434354at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LL30">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000011">
        <property type="entry name" value="SEC14 cytosolic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL_PHOSPHATIDYLCHOLINE TRANSFER PROTEIN SFH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="8">
        <location>
            <begin position="151"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="14"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ93165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="625" mass="71223" checksum="41A9C5AF3E8ADA43" modified="2016-01-20" version="1">MSGHLDRLARPCFEGCSSHDERRDHKSDMEVSEDEKKTRMGSLKKKAIDASSKLRHSLKKKNRRKSGSRVLSVSIEDFRDLEELQAVEAFRQALILDELLPARHDDYHMLLRFLKARKFDIEKAKQMWMDMLQWRKEYHTDTIIEDFEYDELDTVLQYYPHGYHGVDREGRPVYIERLGKVDPNKLMNVTTLERYVQYHVKEFERSFLIKFPACSLAAKRHINSSTTILDVQGVGLKNFSKTARELIMRLQKIDNDNYPETLYQMFIVNAGPGFRMLWGTVKSFIDPKTTSKIHVLGNKYQSKLLEIIDASELPEFLGGTCTCPEYGGCLRGEKGPWKDANILKKVLNGEAQCARQIVTVSNGEETIISYAKSKYQTIRGSDTSTAESGSEAEDVTSPKALRSYISHPKLTPVREEVKMIRATSFSTRMPEYDIPVVDKAVDATWKRELPRKTPFPSKDSSSTSTTRASNWSLGQIVPALMAFVLAILTLFRSVKDLATKRLQDKKDSEEQCSALYPDPVAKGEFRPPSPGPGFAEADLFALVLQRLAELEEKVQTLQEKPSEMPCEKEELLNAAVRRVDALEAELIVTKKALHEALIRQEELLAYVDSKAIAKAQKKKKAMFCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LQW5</accession>
    <name>A0A0Q3LQW5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g14236v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LQW5"/>
    <dbReference type="STRING" id="15368.A0A0Q3LQW5"/>
    <dbReference type="EnsemblPlants" id="KQJ94941">
        <property type="protein sequence ID" value="KQJ94941"/>
        <property type="gene ID" value="BRADI_3g14236v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ94941">
        <property type="protein sequence ID" value="KQJ94941"/>
        <property type="gene ID" value="BRADI_3g14236v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LQW5"/>
    <dbReference type="OrthoDB" id="5948799at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF443">
        <property type="entry name" value="MATH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="4">
        <location>
            <begin position="78"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="285"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="315"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="458"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ94941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="52756" checksum="0AF59599372ADB2E" modified="2016-01-20" version="1">MVVPETTTGNNKALATWALPQYLFYSSSSSSSSSNESPAAAEPEEAEETTSPSSPPRPAPAAEALVVTTSSCTTETAEGVHVFHVDRYRLIKDLGVGKSVRSGDFSVGGYRWAVLFYPSGSEPPKQPGGARHVSAYLELLTPNVDEVRVMFDLSLTAGTHHPWVVAEDFSPADSAELRRRSFFKEGDFRRHASFSTTPPPLRPPRPLPPHHPPRCWGVAEFARGDLAPFIVDGGLVIRCDVTVIKPPRVTPTRRLLQAPTAMPPLGLGEDRRRLLEKEKRDTAGADVEVLVRGESVAAHRALLAARSPVLRKRLHETGNTAVAGDDDEKENDKDGDGKKRRHTAAKRVAVTVDDVEPAVFKAMLHFIYTDELPPESAGEATELLQWLFEAAKAYGVERLPQMCEAVLCARLSKATIKAAAAFARRHGGSCDKLKTACLEFYKATHRVSETAIIGSEQRLKRSLPDGEADDDDSEAAADKRLKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NZV4</accession>
    <name>A0A0Q3NZV4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g03868v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81929.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NZV4"/>
    <dbReference type="FunCoup" id="A0A0Q3NZV4">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ81929">
        <property type="protein sequence ID" value="KQJ81929"/>
        <property type="gene ID" value="BRADI_5g03868v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81929">
        <property type="protein sequence ID" value="KQJ81929"/>
        <property type="gene ID" value="BRADI_5g03868v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NZV4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3NZV4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ81929.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="283" mass="29744" checksum="F842E9F6F8F64B3E" modified="2018-03-28" version="2">MPLSAACSSPSPTMQSLLLRFGGPPDLGIPTTLASPSGLGDKPVDPPSPALLGDDRIYLEDGPNVASSREAAPGLGDSPVADLGCEVLAQVGTAHAAPPPSPRPCALGTPAPHRSDSPRARSLSPTPQRSGGSRPGRSRSSPASVEDPRSVGSRFASPPIHYGRTRTSSARALIDAPRPRNLGEFLAAAKSRTDDVLEKPPARRRQLAPDLLPRRSSWLAGQASGLNSEQRAHRVLLRKLGVVKEDEVPSTEAIGAYRRLFEVPLEEDMVAAILDFWCGTSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PBT8</accession>
    <name>A0A0Q3PBT8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g06536v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_4g06536v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ86593"/>
        <property type="gene ID" value="BRADI_4g06536v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="705709at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ86593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="67" mass="7544" checksum="F6E69238CE4E250F" modified="2016-01-20" version="1">MGQTQSPFMRLPEAEQLLGPVVATSSGNRNRKEQFGKSTKARNKYYKNSTKDDLVLRVTLESITRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RVX7</accession>
    <name>A0A0Q3RVX7_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g32115v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodium</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RVX7"/>
    <dbReference type="STRING" id="15368.A0A0Q3RVX7"/>
    <dbReference type="EnsemblPlants" id="KQK17034">
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        <property type="gene ID" value="BRADI_1g32115v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17034">
        <property type="protein sequence ID" value="KQK17034"/>
        <property type="gene ID" value="BRADI_1g32115v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3RVX7"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RVX7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK17034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="98" mass="10884" checksum="4652F5BD58D9F542" modified="2016-01-20" version="1">MGPFSLCNSQKFIQFQLTVNVAVLIINFVSGISSGDVPLNAFQSISYVSSKSFPSVRILSKYPRSYIPPNVAQVSYVELGDNDIVETRFCWKVMEATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="5">Replication protein A OB domain-containing protein</fullName>
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CF61"/>
    <dbReference type="STRING" id="15368.A0A2K2CF61"/>
    <dbReference type="EnsemblPlants" id="PNT60661">
        <property type="protein sequence ID" value="PNT60661"/>
        <property type="gene ID" value="BRADI_5g03020v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60661">
        <property type="protein sequence ID" value="PNT60661"/>
        <property type="gene ID" value="BRADI_5g03020v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="696691at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CF61">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <location>
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            <end position="258"/>
        </location>
    </feature>
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        <location>
            <begin position="227"/>
            <end position="243"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT60661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="31774" checksum="6778AD82B8351346" modified="2018-03-28" version="1">MVPGCVYYLQYFEVTPAKDRFRPVPHRFELRLARWSTVNEICPAPVDFPIHAYKLRSFQSMIDNIGDETFLVDLIGIVTGVPPLVSVDVKGRQVSKRVVRLTVMRQTAILALWGENADGLDADALVVSSRKEPVIALVMGCTYNLEDSMLSLSASYGSKICINMANPDVVFLRNRRIGSVQCTIEWIIDKGQRFRSLEVENVDVSCLASMIPHVAVELESLPDAKDLGKKDGTGARGAAADKRKDKRQRSGTSKSHVSKKLFTYGAGGRRRATTMLINCSACPNDHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHV8</accession>
    <name>A0A2K2CHV8_BRADI</name>
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        <recommendedName>
            <fullName evidence="4">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100838144</name>
        <name evidence="1" type="ORF">BRADI_5g17830v3</name>
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    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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        </lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHV8"/>
    <dbReference type="EnsemblPlants" id="PNT61618">
        <property type="protein sequence ID" value="PNT61618"/>
        <property type="gene ID" value="BRADI_5g17830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61618">
        <property type="protein sequence ID" value="PNT61618"/>
        <property type="gene ID" value="BRADI_5g17830v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="588330at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CHV8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000066">
        <property type="entry name" value="Acyl-acyl carrier protein thioesterase ATL3 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050563">
        <property type="entry name" value="4-hydroxybenzoyl-CoA_TE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793">
        <property type="entry name" value="4-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793:SF25">
        <property type="entry name" value="OS04G0553100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT61618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="24445" checksum="3E3AEB08E02A702E" modified="2018-03-28" version="1">MQQIGAPVPLANHQFLQHPCRICTAGAGRSNSGHLLMSLHKAYLHRHQFRVAPRTSSTLQAVTTSTVDQNSNIRKDKYFKVEMEVHETELDQYGVVNNVIYTSYIQNGRDKLLESLGISVDSIISKGDALALSELRLKYFAPLKHCQNGDRFVVKTKLMEIKGVRIIFEHIIETLADHKLVLEAKGTAVCLNKDYRPTRVFPDMSTKLLQYFSSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CKS3</accession>
    <name>A0A2K2CKS3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g06055v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CKS3"/>
    <dbReference type="EnsemblPlants" id="PNT62627">
        <property type="protein sequence ID" value="PNT62627"/>
        <property type="gene ID" value="BRADI_4g06055v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62627">
        <property type="protein sequence ID" value="PNT62627"/>
        <property type="gene ID" value="BRADI_4g06055v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CKS3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT62627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="60" mass="6828" checksum="3A6C1294FDDDE88C" modified="2018-03-28" version="1">MDKKETRRCELLMWTKASAPLWLKTKASAPCTGHQNHNEASALPLFRAPDLSKHTLHLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQ22</accession>
    <name>A0A2K2CQ22_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH OST-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104585057</name>
        <name evidence="3" type="ORF">BRADI_4g24700v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239289.1">
        <property type="nucleotide sequence ID" value="XM_010240987.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQ22"/>
    <dbReference type="FunCoup" id="A0A2K2CQ22">
        <property type="interactions" value="675"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CQ22"/>
    <dbReference type="EnsemblPlants" id="PNT64121">
        <property type="protein sequence ID" value="PNT64121"/>
        <property type="gene ID" value="BRADI_4g24700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104585057"/>
    <dbReference type="Gramene" id="PNT64121">
        <property type="protein sequence ID" value="PNT64121"/>
        <property type="gene ID" value="BRADI_4g24700v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104585057"/>
    <dbReference type="OrthoDB" id="549353at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CQ22">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10910">
        <property type="entry name" value="PIN_limkain_b1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="entry name" value="LOTUS domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024768">
        <property type="entry name" value="Marf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14379:SF91">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14379">
        <property type="entry name" value="LIMKAIN B LKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
        <property type="entry name" value="HTH_OST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH OST-type" evidence="2">
        <location>
            <begin position="245"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="672"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="769"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="797"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT64121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="843" mass="92944" checksum="CAC8C04E33D346D9" modified="2018-03-28" version="1">MSQQALLLRFRVQGGLRGFSSQAAERWMGQQAEREEESKAVKVSVWWDFERCHLPPGANPCRVAPRVTAALRSAGVRGPVEITAFGNVNVLPRAVQEGLAATGVAFSHVPRLGGKEGSDRSFMADLVYWIAQNPPPAHFFLISGDKDFANILHRLRMSNYNILLACPSADPSVLCSAATIMWPWEALVNGVGFSPKHFNHPPDGLSSSWYSHYRGALDDPFLQAEPKHSMTLPMHTKKAENPPVVPKFVIHCIRRTLNSYSEGVNIQDLRSELKRNGVPTDKGFFGFKTFTDLLRALPEYVKFIDPLPGDSLPAVVGEKNFKIIESGESNGEVKCLNESKTVKSPSSSVPSSPSDILSAEQKKIPAVDTRSSLSDSLSRDQRKAPPIDFITQSEPPACHMEADAVTASGTPLPGLQGTSKKGLFERIRILWNGPSPTMPETYHSKGSDNVARHGGHNTDQHNRHFETINKNVSRTDNLDVKDLGSHSAVGTSLSNFPSTKLSDLNVKENFANSKNQSSKAENVRGLGETNYGIFSWAARWWSSGKSATQENTDYTDVTDETKEDLDKGSAFVKRPDSASGQQVGAELFEKSYFWDALEQYLLTPHGSKLVSEAKTRHELAHGLQKQGCWPLKGLDKKNLHQLVHLLVSEKKWIQESNSETFHFLLTLPQRGGSAPLPSSKSGGSSPFARPARNRNGQASYKGDENQGNGDDLAWEELGPVSSSSDPRQETDKVPLYQPPTPSDDDFSDDETNAASLQERKDSDDEFREDENHEASQQGRKDESGSSLVKILASWSTSKDDGSRKRDHGVGRLHGTPPRNIMVDCSRSDGSYRQCWRPSSKHHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CVE6</accession>
    <name>A0A2K2CVE6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100827074</name>
        <name evidence="11" type="ORF">BRADI_3g05611v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317500.1">
        <property type="nucleotide sequence ID" value="XM_024461732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CVE6"/>
    <dbReference type="STRING" id="15368.A0A2K2CVE6"/>
    <dbReference type="EnsemblPlants" id="PNT66001">
        <property type="protein sequence ID" value="PNT66001"/>
        <property type="gene ID" value="BRADI_3g05611v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827074"/>
    <dbReference type="Gramene" id="PNT66001">
        <property type="protein sequence ID" value="PNT66001"/>
        <property type="gene ID" value="BRADI_3g05611v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CVE6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03564">
        <property type="entry name" value="ANTH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000005">
        <property type="entry name" value="Clathrin assembly protein AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000003">
        <property type="entry name" value="Putative clathrin assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="10">
        <location>
            <begin position="21"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="307"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT66001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="565" mass="64134" checksum="28267FB155C7A30B" modified="2018-03-28" version="1">MGSWRKAYGALKDSTRVGLAKVNSDFKDLDIAIVKATNHVECPPKERHVRKIFFATSVNRPRADVSYCIYALSRRLSKTKNWTVALKTLIVLHRLLREGDPTFKEEFLAYSYRGNVLHMSNFKDDSSILAWDCSAWVRTYALFLEERLECFRALKYDIETERLMKSPQCSTKAHSRTRTLPCLDLLEHLPALQQLLFRLMGCQPEGAACSNYLIQYALALVLKESFKIYCAINDGIINLVDMFFETPRYDAVKALAIYKRAGMQAENLADFYDFCKQLELARTFQFPTLRQPPPSFLTTMEEYIREAPRPSIKSVQESEERKLLTYSQEAPKEPENPAEAEKEEPAEPKQEQEPGPEPEPEQQPQPLQTTGDLLNLDEEVNPLVTELEEHNALALAIIGPGDHSKPSTCQDLFSCNTSGWELALVTAPSSHTSRAVETKMAGGFDKLLLNSLYEDGARRQQIASMTYNGSLGQANPFETNDPFAMSYSFAPPSTVQLAMATQHQHQHQYNQHQLQQPYFQPQHQYFQQQPQHFSQPAGSYNPFGDPFSDLIAMVPPPRQSSSSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CVJ8</accession>
    <name>A0A2K2CVJ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g06256v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CVJ8"/>
    <dbReference type="FunCoup" id="A0A2K2CVJ8">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66049">
        <property type="protein sequence ID" value="PNT66049"/>
        <property type="gene ID" value="BRADI_3g06256v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66049">
        <property type="protein sequence ID" value="PNT66049"/>
        <property type="gene ID" value="BRADI_3g06256v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CVJ8"/>
    <dbReference type="OrthoDB" id="581290at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF84">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="20"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="284" mass="32042" checksum="5EE84B5B115531B2" modified="2018-03-28" version="1">MGAPVKSSTGIQHYSIYPVVAIHAAALSAAPTPDGAYIVAALTSSQTNIAFWRPGMAHWSPPLHTEVDRRASWANKMIPTATEDGSMEDVVHYRRRDLERFCVLTSTERLFFYEPVITADGTLTMRRTVRIRMFQAVGLTPAGSGEIVARYLVVSGEDLLIVRRYASLPVAPDRIGLHVFTLREEGQPQDSCLWRNCSSLDLRGKVLFLGRGCSRAYTYTTAVHRHPGGYIYLLDAERFHDLTAVDYRCSDTGRCDYRRFLLEIRRVLPRGPASDCSPWIWFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CXR2</accession>
    <name>A0A2K2CXR2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g17320v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CXR2"/>
    <dbReference type="EnsemblPlants" id="PNT66822">
        <property type="protein sequence ID" value="PNT66822"/>
        <property type="gene ID" value="BRADI_3g17320v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66822">
        <property type="protein sequence ID" value="PNT66822"/>
        <property type="gene ID" value="BRADI_3g17320v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CXR2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="95" mass="10016" checksum="11B2F5188BE6CD71" modified="2018-03-28" version="1" fragment="single">SGQAISLVRHLVIVWDPPWPYQGNPEPCCPSRPQDGTTASPPHAPPPAPALPPSSSRPEPCLPLLAPFRRSPASPASARSAPRPAPLLSSHRPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CZH7</accession>
    <name>A0A2K2CZH7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="4">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Plastid-encoded RNA polymerase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_3g27306v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNA polymerase alpha chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CZH7"/>
    <dbReference type="STRING" id="15368.A0A2K2CZH7"/>
    <dbReference type="EnsemblPlants" id="PNT67431">
        <property type="protein sequence ID" value="PNT67431"/>
        <property type="gene ID" value="BRADI_3g27306v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67431">
        <property type="protein sequence ID" value="PNT67431"/>
        <property type="gene ID" value="BRADI_3g27306v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CZH7"/>
    <dbReference type="OrthoDB" id="666954at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06928">
        <property type="entry name" value="RNAP_alpha_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="11">
        <location>
            <begin position="88"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT67431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="282" mass="30899" checksum="5C08E6D148D6C405" modified="2018-03-28" version="1">MAKAVPKIGSRKRVRIGLRRNARFSLRKSAHPQGRVVFWSSAGTCGFKSSRKASPYAGQRTAVDAIHTVGLQRAEVMVKGAGSGRDAALRAIARSGQADTVGIALRRALLGEIEGTCITRAKFGSVPHEYSTIAGIEESVQEILVNLKEIVLRSNLYGVRDASICVKGPKCITAQDIILPPSIEVIDTAQPIANLTEPIDFCIQLQIKRDRGYQTELRKNYQDGSYPIDVVSMPVRNVNYSIFSCGNGNEKHEILFLEIWTNGSLTPKEALYEASRNLIDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D2P3</accession>
    <name>A0A2K2D2P3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100828907</name>
        <name evidence="12" type="ORF">BRADI_3g42190v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D2P3"/>
    <dbReference type="STRING" id="15368.A0A2K2D2P3"/>
    <dbReference type="EnsemblPlants" id="PNT68541">
        <property type="protein sequence ID" value="PNT68541"/>
        <property type="gene ID" value="BRADI_3g42190v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68541">
        <property type="protein sequence ID" value="PNT68541"/>
        <property type="gene ID" value="BRADI_3g42190v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D2P3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01374">
        <property type="entry name" value="KISc_CENP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000016">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021881">
        <property type="entry name" value="NACK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF40">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-7H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11995">
        <property type="entry name" value="DUF3490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="117"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="568"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="873"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="439"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="513"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="568"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="195"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT68541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1102" mass="123457" checksum="D1FA9CD773CBBD92" modified="2018-03-28" version="1">MGRKVFLCPVKEHLFLYTCPLPSFLGALFSPAVCQFPLFVFVFFSAAASKEERKWKRRGDGTGLAASCSFFSFFRARIARPSSGSTPQKPAQGAEGPAMGAGEEEAAMAAAEAKEERIMVSVRLRPLNGREAGDCSDWECVSPTTVMFRSTVPTAYTYDRVFGPDCSTRQVYEEGAKEVALSVVSGINSSIFAYGQTSSGKTYTMTGITEYSVMDIYDHIEKHPEREFILKFSAIEIYNEAVRDLLSHDTTPLRLLDDPEKGTTVEKLIEETLRDKDHLRDLLAMCEAQRETGETALNEASSRSHQILRLTIESSVRQYVGRGKSSTLVACVNFVDLAGSERASQTVSVGRFKEGSHINKSLLTLGKVVRQLSTGRNGHIPYRDSKLTRILHSSLGGNARTAIVCTMSPARTHIEQSKNTLLFATCAKEVVTNAQVNVVISDKALVKHLQRELARLESELKSPESPSCTSHAEALREKDAQIKKLEKQLRELMEEKDTVQSQLNCLLKSDGDDRTAKRWDEHSRSSESLVRNTPEEALSVSDTYGGSYRDQGHAVFDGSYVFSADHDDSSFPDQTRDRTLTSPWHPPSNYSSDGTESYNMKDVAFRTASEVSEEHCREVQCIEIHEHRRCISHELNILPPVDTKLHKPEVVEISRDALPQPDEEQKFGSITKKMVDPIRMYSDKEEQQTEIIANAVEGPVEVHQCESNGFADNVVKLYTCDSSISLDISKPCPHECLTVKRFIMSSKDSALARSQSCRASFTIIPNNWFDDSENTRRTPPDEIFKYPSRRPGKVRRSLYSENDDCQNDTTSVDHSAVSAEVVCDEVVKDMSVSDEGVKDMSVSDEGANDMSADDAEQDILINDVSCVRGLEEKTEKHHEDQPEDFKAEQQAVRDDSTAVKSVKDVGIDSSLSTIESPSRWPINFTRQQLEIIKLWYDCNVSLVHRTYFFLLFKGDPADSVYMEVEHRRLSFLMSTFIASPAAGPGKLHPDFVSSFKNLERERDMLYRQMLRKLGGEGEKESIYSTWGINLSSKQRRLQLSRLVWSRRGDVEHVRQSASLVARLIDPVEPGQQALREMFGLNFTLAPRRSARRSSFSLLGGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D313</accession>
    <name>A0A2K2D313_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g44246v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D313"/>
    <dbReference type="EnsemblPlants" id="PNT68684">
        <property type="protein sequence ID" value="PNT68684"/>
        <property type="gene ID" value="BRADI_3g44246v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68684">
        <property type="protein sequence ID" value="PNT68684"/>
        <property type="gene ID" value="BRADI_3g44246v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D313"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT68684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="100" mass="10630" checksum="6985A9BCFE7D3B7C" modified="2018-03-28" version="1">MYGSTSLLFLNLQLRCPCRRPPPLRVRRPRSPSPARAAANLVPRPPSPRPPPPPEACPAPPAPSEPRSNRPLRNPLLAPSAPPAPPPSRGALPPSAPDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D7Z5</accession>
    <name>A0A2K2D7Z5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC112270983</name>
        <name evidence="8" type="ORF">BRADI_2g11400v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315554.1">
        <property type="nucleotide sequence ID" value="XM_024459786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D7Z5"/>
    <dbReference type="STRING" id="15368.A0A2K2D7Z5"/>
    <dbReference type="EnsemblPlants" id="PNT70404">
        <property type="protein sequence ID" value="PNT70404"/>
        <property type="gene ID" value="BRADI_2g11400v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT70406">
        <property type="protein sequence ID" value="PNT70406"/>
        <property type="gene ID" value="BRADI_2g11400v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT70408">
        <property type="protein sequence ID" value="PNT70408"/>
        <property type="gene ID" value="BRADI_2g11400v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT70409">
        <property type="protein sequence ID" value="PNT70409"/>
        <property type="gene ID" value="BRADI_2g11400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112270983"/>
    <dbReference type="Gramene" id="PNT70404">
        <property type="protein sequence ID" value="PNT70404"/>
        <property type="gene ID" value="BRADI_2g11400v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70406">
        <property type="protein sequence ID" value="PNT70406"/>
        <property type="gene ID" value="BRADI_2g11400v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70408">
        <property type="protein sequence ID" value="PNT70408"/>
        <property type="gene ID" value="BRADI_2g11400v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70409">
        <property type="protein sequence ID" value="PNT70409"/>
        <property type="gene ID" value="BRADI_2g11400v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1297892at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044841">
        <property type="entry name" value="LUX/BOA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31442:SF29">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31442">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="178"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="120"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT70404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="288" mass="32911" checksum="73AC18FE5EE1BCAF" modified="2018-03-28" version="1">MSSMLQPGSPCPRALVVAEYASRRHAIETRLRLLHGFEVEAYSDPTMAMAMRRGSYQRVMVDMFPLRGGAVELLECAFSEMHADAYALSKVGDSYGPLSMNNIETFKRAIKFHISTSIEATPNHRSNNSATHTVSTTLSSSRRRATSREMRRQSETKIQEAEQDGEHSEEEQQPVKKESKKVRLVWTEELKKKFVNVYNKLPPKDAVPTKILKLMNDPTLTRENIASHLQKYRNLKHGEAHTIHKNNSRTNHHTSAEGSFLPHNISQQVPHMMIQVQSRENFQNIFMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DC03</accession>
    <name>A0A2K2DC03_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100830098</name>
        <name evidence="4" type="ORF">BRADI_2g35660v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DC03"/>
    <dbReference type="STRING" id="15368.A0A2K2DC03"/>
    <dbReference type="EnsemblPlants" id="PNT71799">
        <property type="protein sequence ID" value="PNT71799"/>
        <property type="gene ID" value="BRADI_2g35660v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71799">
        <property type="protein sequence ID" value="PNT71799"/>
        <property type="gene ID" value="BRADI_2g35660v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="781822at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DC03">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057235">
        <property type="entry name" value="DUF7913"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057237">
        <property type="entry name" value="DUF7915"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33913">
        <property type="entry name" value="ALEURONE LAYER MORPHOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33913:SF1">
        <property type="entry name" value="DRBM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25500">
        <property type="entry name" value="DUF7913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25502">
        <property type="entry name" value="DUF7915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7913" evidence="2">
        <location>
            <begin position="18"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7915" evidence="3">
        <location>
            <begin position="169"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT71799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="648" mass="71920" checksum="0D048D07B4AF651F" modified="2018-03-28" version="1">MKGGGVAEEMKTAGGDREVVQMEEAVSMLVEYLVLPVLPRGMRAADITPEMEQAVAQQIHAAVILYNCYHRNLSPQLDFADVKQFLLCASLSVGEDLFAFSSIVHEKNPGEDVKPSVTDKAAMDACVVAEALYGSQDFPDMLKWPIAKVAVLLLDPTRKKCLIEYSSDTKGVWSMIEKKIVASAGNSHGSNQSLQSPYMLQQLAFTEVDRRTGMKRSNLRLLGEDLAYSLSTKRTTAMLFIVEYKQTMNRYLREIPIQELISSMTGPLFVNGPFPKTSSVVEHYHILPYKQILRKWSSDAAAEQHEANRKSKMQKGTRKISTPKQNKQAIKSVVTNSNTSCSTDKGTKNSKRTYEAETAAALNVEDRDGESPVRENYPLISVDVDTSKLPTKPRNTKESAATSGGKIVLQADVQMDNKKTQRQSICDDITPDVFPTKAPSIDYVTKNIILEGQNRGVSEKSGGITENNIDQMYDSLQSIQKKRDDLLHKHHILAELSAQCDMDIQTILSEGKMTQIVKSIIDKYKNMGSNMAEVANSSCSGGDGQTLTTKRIKLREAPLLRNKCQELDEISRDSNWILPRYTVLPSEDGMFQASVHLRGVDFDLSIEGGLRMTPQEARWSAAANMILELSRQAEEEKSEDESTISGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DCY1</accession>
    <name>A0A2K2DCY1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100833795</name>
        <name evidence="15" type="ORF">BRADI_2g39720v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DCY1"/>
    <dbReference type="FunCoup" id="A0A2K2DCY1">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DCY1"/>
    <dbReference type="EnsemblPlants" id="PNT72129">
        <property type="protein sequence ID" value="PNT72129"/>
        <property type="gene ID" value="BRADI_2g39720v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72129">
        <property type="protein sequence ID" value="PNT72129"/>
        <property type="gene ID" value="BRADI_2g39720v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="544400at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DCY1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="298"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="370"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="PNT72129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="682" mass="76619" checksum="F5D79A691CEC041A" modified="2018-03-28" version="1">MSSSLSTKYKKWVALLRQETGTAILTSAPDVKGGDDVCPPFSCGHLQDISYPFRRQGDPVECGVKEYELICSSSGKATIQINTGTYYVTAINNGSKYFSVMDANFHTSSSCPLPMWKPFNCYRYNSKTDSYVFNLCVEEFYIACFANCSRAITNNSVYKPVDCLSANNSHVYVLVSELGCSVHELEPYCGYLAMSPLSFGDNHSSDWLQPQNTSYEDITQLIRKGFVVGFPGETTTRPWRRGVCVTINRCLNNSISYFKEQTSGGSIMNWTHAFFWSEIHFLECVITSSNNYYTTRSLLVVTTIVSSIAILKFIFVLCRFVLAPMVVWIFLAYKYWKTRITIDVVEKFLRIQQMIGPTRYAYTDITAITGHFRDKLGQGGYGSVYKGVLLPGDVHVAVKMLEGNSSCNGEDFISEVSTIGRIHHVNVVRLVGFCSEEMKRALVYEYMPHGSLDKYIFSAEKSFSWDKLNEIALGIARGINYLHQGCDMQILHFDIKPHNILLDSNFVPKVADFGLAKLYPRDNSFVPLSALRGTIGYIAPEMISRSFGVISSKSDVYSFGMLLLEMAGGRRNADPNAARSSQAYYPSWVYDQLTQQEAGEISPAADMHELEKKLCVVGLWCIQMKSHDRPTMSEVIEVLEAGAHGLQMPSRPFFCDEEHIHVEDSYHFASELTAVSEEDGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGF1</accession>
    <name>A0A2K2DGF1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g57406v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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    </evidence>
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        <source>
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</entry>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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</entry>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
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            <strain evidence="1 2">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="1">Bd21</strain>
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        <source>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">Bd21</strain>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DNP9"/>
    <dbReference type="STRING" id="15368.A0A2K2DNP9"/>
    <dbReference type="EnsemblPlants" id="PNT75908">
        <property type="protein sequence ID" value="PNT75908"/>
        <property type="gene ID" value="BRADI_1g41651v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75908">
        <property type="protein sequence ID" value="PNT75908"/>
        <property type="gene ID" value="BRADI_1g41651v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DNP9"/>
    <dbReference type="OrthoDB" id="7692063at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45786">
        <property type="entry name" value="DNA BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45786:SF68">
        <property type="entry name" value="OS02G0701800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="2">
        <location>
            <begin position="371"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT75908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="484" mass="57499" checksum="D881D38FB886A104" modified="2018-03-28" version="1" fragment="single">TKKEKRERERDAKRTDEEKQEKLKKRRESYKQKTDEEKQEQLEKRREAYEQKKEKVTEQKTKQQAQHRRRYANMQPEQKKARINQIGANRELKRSTPSRDSIAMENPAYITAEQEVNISPLKVRRRKTVTPGERQTLVHRRNKEFSTRQRKTVQVSVQEDTSKMKTHNDEIEATNKNVDEQPQPFFNENERHPKHLELYFYDDDPSLEHRYRRCRKETYEQDKHVVGIITEILRENPYSKQFRSLGQAEDLDDYRLILNLDQRLDQRTYNAPITSEVAAVWIKGNQRRNTFDKNVIIHGNNNEIQIIKSYYGCYDPLSYPLFFPRGELGLHSKIPKANAEEEDMKKAGAEENDDDDDPDATSRLWVTMKEYYCYKLHMRPNIFNPILYGGRLLQQLDYMFHHQKEIRADLYQGLLDSIKAREQDGNAIGKRTVLASSFIGGPRDKLRRYLDAMALVRKYGKPDIFLTMTCNPNWEEIKCELECG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DPT6</accession>
    <name>A0A2K2DPT6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100840482</name>
        <name evidence="5" type="ORF">BRADI_1g46804v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DPT6"/>
    <dbReference type="STRING" id="15368.A0A2K2DPT6"/>
    <dbReference type="EnsemblPlants" id="PNT76287">
        <property type="protein sequence ID" value="PNT76287"/>
        <property type="gene ID" value="BRADI_1g46804v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76287">
        <property type="protein sequence ID" value="PNT76287"/>
        <property type="gene ID" value="BRADI_1g46804v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="49309at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DPT6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052435">
        <property type="entry name" value="YY1-Transcr_Regul"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16088:SF3">
        <property type="entry name" value="GON-4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16088">
        <property type="entry name" value="YY1 ASSOCIATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="211"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="619"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="913"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1053"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1105"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="221"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="641"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="936"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1053"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1063"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1140"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1159"/>
            <end position="1174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT76287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1183" mass="131932" checksum="C0E96BB8FD28CBC3" modified="2018-03-28" version="1">MEVDDDVEDDDMDFNPFLREESPSETSSSLTSEAECEETSFENQPSTEVYLHNSLGNENTGDCARPQKRLSSKGACKENAPESTSTQFYCEHGEGHSNVLEKEPLQNEASFAPTVQSSHQQATGSEEEDAICRRTWARYSLANYALEELETFLQESDDEGGLQNVDEEEEYCKFLAAVLSGEGDDSQACQGDETQDEDENDVGFELEIEEALESDGDENAENCKNRNGRNDKDGHRPQTRQKRLELSRSTNCHHESTNSTLRPILPYISPALLAPGHACGQQYPSRNVNLPSSLVSVTGAAVMGGFTDQQLGQWHMLIYEHVQLMIQIFSLCVLDPSKQRVAADLKKMIIELVGYCDQALSSPIMSILDVSPFHLVLNYLSDVAAAVVKYRKSHLDGTAEKNRSRKEPLFPTPVLSTTKDATNVSQGRSNIVSTASPASRGQLQPKKSLAATLVENTKKESVALVPFDIARLAQRFYPLFNFSLFPHKPPPAVMVNRVLFTDAEDRLLALGLLEYNNDWGAIQKRFHQIFVRQKNRSSSKAPDNPVKDVRRMKNSPLSVEEVQRIEEGLKIFKNDWTSVWMFLLPHRDPALLQRQWRVATGVQLSYSKSETLKEKRRSYEAKRRKLRASVPDSQEVCGQEADNDAPEGVENDDDSYVNEAFLADTDNRSMSMMRQMGNSLDDECGAAHGCFEQDNRNGRKHGVSASCIPFSSCASDGPSSSKRVSGGTLDELQGSLRKEKGGHVVKLAPDLPPVNLPPSVCVISQKEFHQSAAHFNGTSDNTAKDLFPVPPPTFTESVYRQLNLFPDHSTSDRLQQHVVNNRNTVDDGAEQDFPMHPLLFQFPQEVNLSYSHPVQNLISNSRNYDLFPFEKVQVEKSNRQTTDDMEEGAPNNANTIDFHPLLQRTEAEMREEVPEEDGHQFANQSDCRMRKPPVDDQSTARQASTSPCEGEHRIDLQASISPCGRENNIDLDIHLCSSTDFMSAKDFRSTKSSIQPEGSMKDRASISIIEPGNVCSYHDTEGPSEEAIQGIVMEQEELSDTEEDSQHVEFECEEMDDSEEEQAQGTEPCLTQNKGTSTSVVCSEFQESNDQCQIQQGLVHVVKQGVTSPEKLHGSSSARSAKAKLKPEHAKRTRSRKHQQVSTSRTSEPSLAKTRRPKAQKEEAGAEYKSSDSRRSRKSPAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQ29</accession>
    <name>A0A2K2DQ29_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g47614v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQ29"/>
    <dbReference type="EnsemblPlants" id="PNT76382">
        <property type="protein sequence ID" value="PNT76382"/>
        <property type="gene ID" value="BRADI_1g47614v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76382">
        <property type="protein sequence ID" value="PNT76382"/>
        <property type="gene ID" value="BRADI_1g47614v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DQ29"/>
    <dbReference type="OrthoDB" id="693277at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="125" mass="14670" checksum="931EBA2931483DEF" modified="2018-03-28" version="1">MAKMGESRTGGRHSRFLVHKPSSLPPKVPSRYGNACWEYICPTFSAKQNVHDNVIHLKEELKVPFHLEIMVLCTWSIWKSRNDFIFNNIQPSFYRCRRLFKEELILSFIDLGERNMPTLSLDLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DUM9</accession>
    <name>A0A2K2DUM9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC104582403</name>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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            <strain evidence="2">Bd21</strain>
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        <source>
            <dbReference type="EMBL" id="PNT78330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="9193" checksum="849AC5B7434FA457" modified="2018-03-28" version="1">MGCSSSMSMALLLLLLVAAADLTTVTRSAWVGDYASNHGCGVTAVAELCDPDDESANRACSDACHYSGCGGGRCVFLPSSSGGASRGCHCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNA8</accession>
    <name>I1GNA8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Iron hydrogenase small subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100834252</name>
        <name evidence="7" type="ORF">BRADI_1g08620v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NARF family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559595.1">
        <property type="nucleotide sequence ID" value="XM_003559547.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNA8"/>
    <dbReference type="FunCoup" id="I1GNA8">
        <property type="interactions" value="1702"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GNA8"/>
    <dbReference type="EnsemblPlants" id="KQK13213">
        <property type="protein sequence ID" value="KQK13213"/>
        <property type="gene ID" value="BRADI_1g08620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834252"/>
    <dbReference type="Gramene" id="KQK13213">
        <property type="protein sequence ID" value="KQK13213"/>
        <property type="gene ID" value="BRADI_1g08620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834252"/>
    <dbReference type="eggNOG" id="KOG2439">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018240_0_0_1"/>
    <dbReference type="OMA" id="GYLHHVL"/>
    <dbReference type="OrthoDB" id="10253113at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GNA8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070482">
        <property type="term" value="P:response to oxygen levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000042">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.950.10">
        <property type="entry name" value="Fe-only Hydrogenase (Larger Subunit), Chain L, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050340">
        <property type="entry name" value="Cytosolic_Fe-S_CAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003149">
        <property type="entry name" value="Fe_hydrogenase_ssu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11615">
        <property type="entry name" value="NITRATE, FORMATE, IRON DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02256">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00902">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="Fe-only hydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron hydrogenase small subunit" evidence="6">
        <location>
            <begin position="404"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK13213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="471" mass="52084" checksum="3708D19B4250138B" modified="2012-06-13" version="1">MSSSRFSPALQASDLNDFIAPSQDCVISLNKSSSAARRLPIKQKEIVVSNKPPEDSVKISLKDCLACSGCITSAETVMLEKQSLDDFVSRINSGKAVIVSVSPQSRASLAAFFGLSQSQVFRKLTALFKSMGVKAVYDTSSSRDLALIEACNEFISRYQLKQSSNGKEVGTSLPLLSSACPGWICYAEKTLGSYILPYISSVKSPQQVIGAAIKHHMVEKLGLKPYDVYHVTVMPCYDKKLEAVRDDFVFSVEEKEITEVDSVLTTGEVLDLIQSKSFDFKTMEESPLDRLLTNVDEDGHLYGVSGGSGGYAETIFRYAARALFNREIEGPLDFKILRNSDFREVTLEVDGKPVLKFALCYGFRNLQNIVRKIKMGKCEYQFIEVMACPSGCLNGGGQIKPVKGQSAKDLIQQLEGVYIQDVSISNPFDNPIATRLYDDWLVQPGSENAKKYLHTKYHPVVKSVASQLQNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNQ4</accession>
    <name>I1GNQ4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100842286</name>
        <name evidence="2" type="ORF">BRADI_1g09890v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559469.2">
        <property type="nucleotide sequence ID" value="XM_003559421.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNQ4"/>
    <dbReference type="FunCoup" id="I1GNQ4">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13397">
        <property type="protein sequence ID" value="KQK13397"/>
        <property type="gene ID" value="BRADI_1g09890v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842286"/>
    <dbReference type="Gramene" id="KQK13397">
        <property type="protein sequence ID" value="KQK13397"/>
        <property type="gene ID" value="BRADI_1g09890v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842286"/>
    <dbReference type="eggNOG" id="ENOG502R585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1186662_0_0_1"/>
    <dbReference type="OMA" id="FRGCGIW"/>
    <dbReference type="OrthoDB" id="611517at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33696:SF18">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33696">
        <property type="entry name" value="T22J18.15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK13397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="257" mass="27270" checksum="8DDA4BC2D0E6E6F6" modified="2012-06-13" version="1">MMSRRTRRADLQQQKQPVPEFAGTNTIVSNPIFSYEDGSGSTQLKPVDDVLLGCGYKEPAGSGQDDVIPDAVFPAAGAGVVKVKPLPLPPSMAHRLPASSSRAGPAALVMMARSQHGGWVLTRRAPHHDPFLAAYVACTKSGGKDDSSPATVKQQHRQRKKNETNNNTRGDDALRGCGIWSGWAAAAGAKKKKYAGAMSCKHGSAVAVAVQRKGDDPAQALASPGHPRLHLSRQLVVIPARKKALVVLPRGRAQGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPD2</accession>
    <name>I1GPD2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_1g11860v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPD2"/>
    <dbReference type="STRING" id="15368.I1GPD2"/>
    <dbReference type="EnsemblPlants" id="KQK13689">
        <property type="protein sequence ID" value="KQK13689"/>
        <property type="gene ID" value="BRADI_1g11860v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13689">
        <property type="protein sequence ID" value="KQK13689"/>
        <property type="gene ID" value="BRADI_1g11860v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RRQR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057948_1_0_1"/>
    <dbReference type="InParanoid" id="I1GPD2"/>
    <dbReference type="OMA" id="NWVIMGD"/>
    <dbReference type="OrthoDB" id="687653at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052422">
        <property type="entry name" value="Auxin_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47986:SF32">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47986">
        <property type="entry name" value="OSJNBA0070M12.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094005" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="10">
        <location>
            <begin position="21"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="11">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK13689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="305" mass="32022" checksum="C303B6C9437B278B" modified="2012-06-13" version="1">MSIAVILLVVMLGVSRPAGAATDPKDVVAMQALAKSTGAATSLGWGVKSADPCDGTWVGVRCNNDDLSHVTSIVASRAGLDGSLPGNELSMLEFLTELDLSFNKLGDELPTVPIPLKHLTALDLRANSFSKMPDFFFRGFPALETVVLDDNAVVMGDMTEAPACPGLRSFSANNFTIYGELPIYFGNKTLFPALESLSVARNQLTFGVSPYFGKDSGIKFLDISGQIDDGVGKLSGRLDFVSGMTSLVEIRVAGNGFYGPLPDVSGLVNLKVFDAADNKLCGPVKFPPAVAVNVAGNPKVGNSCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQP0</accession>
    <name>I1GQP0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF1230 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100828869</name>
        <name evidence="5" type="ORF">BRADI_1g15830v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562361.1">
        <property type="nucleotide sequence ID" value="XM_003562313.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQP0"/>
    <dbReference type="FunCoup" id="I1GQP0">
        <property type="interactions" value="748"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GQP0"/>
    <dbReference type="EnsemblPlants" id="KQK14379">
        <property type="protein sequence ID" value="KQK14379"/>
        <property type="gene ID" value="BRADI_1g15830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828869"/>
    <dbReference type="Gramene" id="KQK14379">
        <property type="protein sequence ID" value="KQK14379"/>
        <property type="gene ID" value="BRADI_1g15830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828869"/>
    <dbReference type="eggNOG" id="ENOG502QS25">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071172_0_0_1"/>
    <dbReference type="OMA" id="DSTLYSW"/>
    <dbReference type="OrthoDB" id="192326at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009631">
        <property type="entry name" value="CGLD27-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34214">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34214:SF3">
        <property type="entry name" value="PROTEIN CONSERVED IN THE GREEN LINEAGE AND DIATOMS 27, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06799">
        <property type="entry name" value="CGLD27-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="18"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK14379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="34038" checksum="9DDF5112DCC45B35" modified="2012-06-13" version="1">MLLRLKIVTVQPAAASLHHGSTAPRRRLALQGGGRASKRTTRTKRLKTVAKALKEEGEPESSRSRFPGGGPSWDPGLEIGVPYDQRPVNEYSALKDSILYSWAELSPGSFFMRLGSLCLVTFTVLAAPISAASFSPGKDPLKFVLAAGIGTLLLVSLVVLRIYLGWSYVGDRLLSAVVPYEETGWYDGQMWVKPPEVLARDRLLGSYKVKPVINQLKQTLVGTGALLVGAVSLFAFAAPVQDFVHSFNAAPSAASSSKPTMRREELLRLPAEVKTDDDLAAAAAEAAGGRPVYCRDRYYRALAGGQYCKGEDLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRZ8</accession>
    <name>I1GRZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anaphase-promoting complex subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100829266</name>
        <name evidence="11" type="ORF">BRADI_1g20320v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the APC13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562618.1">
        <property type="nucleotide sequence ID" value="XM_003562570.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235097.1">
        <property type="nucleotide sequence ID" value="XM_010236795.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRZ8"/>
    <dbReference type="STRING" id="15368.I1GRZ8"/>
    <dbReference type="EnsemblPlants" id="KQK15052">
        <property type="protein sequence ID" value="KQK15052"/>
        <property type="gene ID" value="BRADI_1g20320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829266"/>
    <dbReference type="Gramene" id="KQK15052">
        <property type="protein sequence ID" value="KQK15052"/>
        <property type="gene ID" value="BRADI_1g20320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829266"/>
    <dbReference type="eggNOG" id="ENOG502S8ST">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183994_0_0_1"/>
    <dbReference type="OMA" id="ALENHRH"/>
    <dbReference type="OrthoDB" id="25675at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010229">
        <property type="term" value="P:inflorescence development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061614">
        <property type="term" value="P:miRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048827">
        <property type="term" value="P:phyllome development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010152">
        <property type="term" value="P:pollen maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008401">
        <property type="entry name" value="Apc13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28672">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28672:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05839">
        <property type="entry name" value="Apc13p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="23"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK15052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="68" mass="7583" checksum="EE38A20DC9F37794" modified="2012-06-13" version="1">MGDQSLSLGVLIDVVDEQWMRDTLPADDIPLPPQMTVKTEETEDPAPANQESQPAQGDVWRDFAMENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I1GUR9</accession>
    <name>I1GUR9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100823013</name>
        <name evidence="1" type="ORF">BRADI_1g28680v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GUR9"/>
    <dbReference type="STRING" id="15368.I1GUR9"/>
    <dbReference type="EnsemblPlants" id="PNT75209">
        <property type="protein sequence ID" value="PNT75209"/>
        <property type="gene ID" value="BRADI_1g28680v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75209">
        <property type="protein sequence ID" value="PNT75209"/>
        <property type="gene ID" value="BRADI_1g28680v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S8EV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2336520_0_0_1"/>
    <dbReference type="OrthoDB" id="1894836at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GUR9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT75209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="98" mass="10852" checksum="5E2031A2487405C3" modified="2012-06-13" version="1">MEVPAARVATAKEVEKKVELMKEVRAREVAIGELNNLPPSRAAYQKTCNIFFRKNIKTAVISEQSSSMNFLSKGGRLTLTNSQRSHYFTSLASSFPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1GVE3</accession>
    <name>I1GVE3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100832392</name>
        <name evidence="4" type="ORF">BRADI_1g30420v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560341.1">
        <property type="nucleotide sequence ID" value="XM_003560293.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GVE3"/>
    <dbReference type="STRING" id="15368.I1GVE3"/>
    <dbReference type="EnsemblPlants" id="KQK16757">
        <property type="protein sequence ID" value="KQK16757"/>
        <property type="gene ID" value="BRADI_1g30420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832392"/>
    <dbReference type="Gramene" id="KQK16757">
        <property type="protein sequence ID" value="KQK16757"/>
        <property type="gene ID" value="BRADI_1g30420v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832392"/>
    <dbReference type="eggNOG" id="ENOG502S03M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055715_3_0_1"/>
    <dbReference type="OMA" id="PVTHPPK"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01958">
        <property type="entry name" value="HPS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027923">
        <property type="entry name" value="Hydrophob_seed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051636">
        <property type="entry name" value="Plant_LTP/defense-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31731">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14547">
        <property type="entry name" value="Hydrophob_seed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094134" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="3">
        <location>
            <begin position="191"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK16757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="274" mass="27925" checksum="5D5791537EEF98B9" modified="2012-06-13" version="1">MEASRTQKQLAFLLILLLSTAAAAASAPAATVACDCDKPSHSHPPKTKPSFPSPKTGPTKPAPKPKPKGPSYPSPSPAIRPPKPKTPAPVVVGPPKGRPVPRPPVYSPPKGPITVPPVVGPPKGPVVSPPVVYPSPPVTAPPVTYPSPPITAPPITYPSPPVTAPPVTYPTPPAVTTPCPPPPPTPATLTCPVDSLKIGACVDLLGGLVHVGLGDPAVNTCCPLLAGLVELEAAVCLCTTIRLKLLNINLYLPLALQLLLTCGKTPPPGYTCSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWQ0</accession>
    <name>I1GWQ0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100823937</name>
        <name evidence="2" type="ORF">BRADI_1g34250v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010227428.1">
        <property type="nucleotide sequence ID" value="XM_010229126.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWQ0"/>
    <dbReference type="STRING" id="15368.I1GWQ0"/>
    <dbReference type="EnsemblPlants" id="KQK17406">
        <property type="protein sequence ID" value="KQK17406"/>
        <property type="gene ID" value="BRADI_1g34250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823937"/>
    <dbReference type="Gramene" id="KQK17406">
        <property type="protein sequence ID" value="KQK17406"/>
        <property type="gene ID" value="BRADI_1g34250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823937"/>
    <dbReference type="eggNOG" id="ENOG502S0KJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098106_0_1_1"/>
    <dbReference type="OMA" id="HEEDGLR"/>
    <dbReference type="OrthoDB" id="625231at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF13">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK17406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="128" mass="14228" checksum="13C30FA1F24ECB7A" modified="2012-06-13" version="1">MMVMGYFRAPKKRGSRKKNVNSEEAGALREALLEQPAGVGGEGDGGVPKGYFAVYVGEEEEEAMEPRRFVVPTGYLREPAFRELMERAADEFGFAQAAGLRVPCALDDFEDLLRRLRCKNNGKKKPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWY1</accession>
    <name>I1GWY1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100831110</name>
        <name evidence="8" type="ORF">BRADI_1g34890v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563547.1">
        <property type="nucleotide sequence ID" value="XM_003563499.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010227464.1">
        <property type="nucleotide sequence ID" value="XM_010229162.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWY1"/>
    <dbReference type="STRING" id="15368.I1GWY1"/>
    <dbReference type="EnsemblPlants" id="KQK17507">
        <property type="protein sequence ID" value="KQK17507"/>
        <property type="gene ID" value="BRADI_1g34890v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK17508">
        <property type="protein sequence ID" value="KQK17508"/>
        <property type="gene ID" value="BRADI_1g34890v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831110"/>
    <dbReference type="Gramene" id="KQK17507">
        <property type="protein sequence ID" value="KQK17507"/>
        <property type="gene ID" value="BRADI_1g34890v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17508">
        <property type="protein sequence ID" value="KQK17508"/>
        <property type="gene ID" value="BRADI_1g34890v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831110"/>
    <dbReference type="HOGENOM" id="CLU_077522_0_0_1"/>
    <dbReference type="OrthoDB" id="1506770at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GWY1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013260">
        <property type="entry name" value="mRNA_splic_SYF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264">
        <property type="entry name" value="GCIP-INTERACTING PROTEIN P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264:SF6">
        <property type="entry name" value="OS06G0711100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="33"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="96"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="214"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK17507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="376" mass="40003" checksum="3506CF05F7E6249E" modified="2012-06-13" version="1">MGDEARGRAVNPNCPNAVNPFHRCADYCPVAAPAVKPRTPPRGRAAQNGTVHSDGEEAERGGRQRGAVNPDCPNAANPFHRCAEYCPVAAPAVKPPPPPPGRAAQNGTVHSDGEEAERGEVYPDRSNAASAAKPPPLPPGVAAQNGGSAHSDGELQPKTRRRDRAGGSGGLPLYVFLREGSDGEGKKVDPRCPNAPNPFHVCTDHCLAKMVEAGRSSEGGKSPISLFSRRSNRSTSSSEDGSIKSGGSKKTDPKCPNAGNPFHECGEHCTAKMKEVEKQKRTDKKSPRRKGGKDIAVMPNWKVDPRCPNAGNPFHICAQYCFDHLNEAAQKPSSKPDTKGKAVMKAAQTGEINPDCVNASNPYHKCGEYCKRNGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXQ1</accession>
    <name>I1GXQ1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100845352</name>
        <name evidence="19" type="ORF">BRADI_1g37160v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560627.1">
        <property type="nucleotide sequence ID" value="XM_003560579.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXQ1"/>
    <dbReference type="STRING" id="15368.I1GXQ1"/>
    <dbReference type="EnsemblPlants" id="KQK17850">
        <property type="protein sequence ID" value="KQK17850"/>
        <property type="gene ID" value="BRADI_1g37160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845352"/>
    <dbReference type="Gramene" id="KQK17850">
        <property type="protein sequence ID" value="KQK17850"/>
        <property type="gene ID" value="BRADI_1g37160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845352"/>
    <dbReference type="eggNOG" id="ENOG502QTIM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024953_2_0_1"/>
    <dbReference type="OMA" id="KMEVYLF"/>
    <dbReference type="OrthoDB" id="408788at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000008">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000002">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094182" description="glucan endo-1,3-beta-D-glucosidase" evidence="17">
        <location>
            <begin position="24"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="18">
        <location>
            <begin position="367"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQK17850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="484" mass="52656" checksum="C59CC24403535C0D" modified="2012-06-13" version="1">MAPLLRLLAGAAALLVAVSHASAWSSAVDVGVNWGSQLTHPLLPSSVVKMLKDNGIMKVKLFDADPWPVEALLDSGIEVMLGIPNDMLEIMSSYGSAQDWVKENVTAYGDKLKLKYVAVGNEPFLKSYNGTFMKTTVPALKNIQKALDAAGLGDKVKATVPLNADVYVSPDDKPSSGQFRPDIDDVMTDMVKFLHDHGAPFVVNIYPFLSLYQSDDFPFEFAFFDGGRNIQDKDGVSYSNVFDANYDTLVSALKKAGVGGLKVVVGEVGWPTDGNKNANMKLARRFYDGLMKKLAKNEGTHLRPGKLDVYLFGLFDEDLKSIAPGNFERHWGILTYDGKPKFPMDLSGQGHDKLLAGVSGVQYLPHQWCVLDDEAKAQDKLPGNIQYACAGGDCTALGYGCSCDGLDEKSNISYAFNMYFQMQDQDVRACDFDGLANITDKNASTKGCLFPVQIISAGGRMAPALRWTTLLALVAHVLVMGFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZQ3</accession>
    <name>I1GZQ3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100844834</name>
        <name evidence="14" type="ORF">BRADI_1g45620v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560909.1">
        <property type="nucleotide sequence ID" value="XM_003560861.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZQ3"/>
    <dbReference type="STRING" id="15368.I1GZQ3"/>
    <dbReference type="EnsemblPlants" id="KQK18942">
        <property type="protein sequence ID" value="KQK18942"/>
        <property type="gene ID" value="BRADI_1g45620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844834"/>
    <dbReference type="Gramene" id="KQK18942">
        <property type="protein sequence ID" value="KQK18942"/>
        <property type="gene ID" value="BRADI_1g45620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844834"/>
    <dbReference type="eggNOG" id="KOG1001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_2_3_1"/>
    <dbReference type="OMA" id="CHPHLIN"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000068">
        <property type="entry name" value="SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF24">
        <property type="entry name" value="HELICASE-LIKE TRANSCRIPTION FACTOR CHR28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="472"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="794"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="893"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="533"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="558"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK18942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1070" mass="117889" checksum="BECF25D56D5697CF" modified="2012-06-13" version="1">MLASNMNNDNDNVIDLISDSDDDFDFDSDDPTSTSVTSVRNGEGQPIRLQDEDLARTMLSTPSSSRPLKNSNGQYRTLPPSFANGIEPLKNSNGQYRTLPPSFANGIDIKKARYTRGSGDRTYPHSSSYVGSPYDSARATLTSNRIASVVKELNGSALDANDNDKMILPSSFSNGSTAKSINPNVASDSRRLPSRFANGNSQKMVENMMESNDAKQIGQPSSSKFPTQNLSLSNAQKVITDDDDDDDIYVYGGTSSNRVLPSSFGGNNSANNTDLVNGNGMQARPNLESRFLDSEERAVYQEALQNISLDKKEDDLPEGYLSVSLLKHQKMALAWMVSKENSSHCAGGILADDQGLGKTVSTIALIQKQRIPQSKFMSADSDALKSEALNLDEDDETVTVVDKGEQILNNEPKELGASLSSTAASISGVKPCISEIEVPDRMAESKVECKKKTKTGTSSASSMHSMTRPAAGTLVVCPASVLKQWANELTDKVGESAKLSVLVYHGGARTKNPSELAKYDVVVTTYTIVANEVPKQNADDDPDQKNAEESSAGNKTKPSSKSKKRKKKLKDSDIDLNSGPVARVRWFRVVLDEAQTIKNFRTQVAKACCGLRAKRRWCLSGTPIQNAIDELYSYFRFLKYDPYSTYSSFCTMIKHPIARDAVHGYKKLQTVLRIVLLRRTKETKINGEPIINLPPKTINLNKVDFTKEERAFYSTLEERSQQQFKEYAAAGTLNQNYANILLLLLRLRQACDHPLLVKGHQSVFKGDGSIERARQLPKDLVIDLLAKLEVSSLCAVCRDTPEDAVVAMCGHIFCYQCIHERITTDENMCPTPNCRTTLSTESVFSSGTLRICIAGNTCTYATASSSADDELSSISQSSYMSSKIRATVDELNTIINTHAITDSDTSESNPSQVAPVKAIVFSQWTGMLDQLELSLNNNLIRYRRLDGTMSLNLRDRAVKDFNTDPEVRVMIMSLKAGNLGLNMVAACHVILLDVWWNPYAEDQAIDRAHRIGQTRAVTVSRLTIKDTVEDRILALQEEKRAMVNSAFGEDKSGGHATRLTVEDLRYLFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZR7</accession>
    <name>I1GZR7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100844857</name>
        <name evidence="4" type="ORF">BRADI_1g45747v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GEM family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003564036.4"/>
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    <dbReference type="AlphaFoldDB" id="I1GZR7"/>
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    <dbReference type="EnsemblPlants" id="KQK18984">
        <property type="protein sequence ID" value="KQK18984"/>
        <property type="gene ID" value="BRADI_1g45747v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844857"/>
    <dbReference type="Gramene" id="KQK18984">
        <property type="protein sequence ID" value="KQK18984"/>
        <property type="gene ID" value="BRADI_1g45747v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844857"/>
    <dbReference type="eggNOG" id="ENOG502QUT3">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_063785_1_1_1"/>
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    <dbReference type="OrthoDB" id="640718at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GEM-like"/>
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        <property type="entry name" value="GRAM"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR31969">
        <property type="entry name" value="GEM-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="167"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK18984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="291" mass="31110" checksum="695CEB6B7F60F243" modified="2012-06-13" version="1">MQPAAEMQPPAGSPTASQSDTPQPPPPSDPSPPPSPAPAPAPAPEAADPPAPAPALAPVQLQAQQKSVTWSEKLTSESPTHVPAAAAAEASQYVSRGPASSSSKGAVEAMKDTLSRWGKSMGETTKMVESLSRDTWQHFKTGPSFTEAAMGRLAQGTKVLAEGGYDKIFKQTFEVLPDEQLKISYACYLSTSAGPVMGVMYISTAKIAFCSDNPLSYKAGNKTEWSYYKVVIPLHQLRSANPSVSKVNSAEKYIQVVSVEGHEFWFMGFLMYDKAVATLQEALDCSRELQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1H088</accession>
    <name>I1H088_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Origin recognition complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100821084</name>
        <name evidence="20" type="ORF">BRADI_1g47050v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">Bd21</strain>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the origin recognition complex (ORC) composed of at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle and development dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Binds unmodified and methylated histone H3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ORC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564177.1">
        <property type="nucleotide sequence ID" value="XM_003564129.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H088"/>
    <dbReference type="STRING" id="15368.I1H088"/>
    <dbReference type="EnsemblPlants" id="KQK19227">
        <property type="protein sequence ID" value="KQK19227"/>
        <property type="gene ID" value="BRADI_1g47050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821084"/>
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        <property type="protein sequence ID" value="KQK19227"/>
        <property type="gene ID" value="BRADI_1g47050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821084"/>
    <dbReference type="eggNOG" id="KOG1514">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_010923_0_0_1"/>
    <dbReference type="OMA" id="RVCFKAG"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009744">
        <property type="term" value="P:response to sucrose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd04718">
        <property type="entry name" value="BAH_plant_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000082">
        <property type="entry name" value="Origin recognition complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Origin recognition complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Origin recognition complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Origin recognition complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041083">
        <property type="entry name" value="AAA_lid_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF23">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17872">
        <property type="entry name" value="AAA_lid_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 16" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="18">
        <location>
            <begin position="159"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="BAH" evidence="19">
        <location>
            <begin position="217"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="339"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KQK19227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="818" mass="92544" checksum="FA4B3A0CFDC77354" modified="2012-06-13" version="1">MDLSATPTRSKSKPRSTPSKTIAASAAAQMDLSTPSKPTTRRKSKSVSSPPSIAPATPSTVRRSRRLLETPTRRLLETPTKDLVETPAKPAPAPTLKRKRAAPSPKTPIQAEPKRQRRLPKKKAHYRKVVYDGGEFAAGDDVYVKRREGAESDEEEPEEEECRVCFHAGGEVMVECDACLGGFHLRCVRPPLRRVPEGDWACPYCEAERAGRAIERPKQPVGKSIRRTAKEKLLSSDLWAARIESLWREPDGTFWAKVRWYTIPEETAAGRQPHNLRRELYRTNDLADIEMETILRHCYVMSPKEFRDASNEGDDVFYCEYEYDVHWHNFKRLADIDDEPETQEDPSDEPYNAGNDYNSDTDEDSEFEEEDGPAKRCSARKNQSHQFAANSRKGRIYGLQKIGIRKIPEHVRCHQKTDLEKAKATLLLATLPKSLPCRDKEMEEISTFVKDAICNDQCLGRCLYIHGVPGTGKTMSVLAVMRRLRSELDSGALRPYCFIEINGLKLASPENIYKVIYEQLSGHRVGWKKALHYLTEHFSDGTKIGKQTSQPIILLIDELDLLLTRNQSVLYNVLDWPTKPNSNLVVIGIANTMDLPEKLLPRISSRMGIQRLCFGPYNYRQLQEIITSRLKGIDAFEDQAIEFASRKVAAMSGDARRALEICRRAAEFADYRIKQFQQSGQAPSSANRGNGVVCMGDVEDAIQEVFQAPHIQVMKNCPKFGKIILAAMVHELYRSGLGEVLFDKLAATVLSWCHVNRELLPGYDTLLKICCKLGESKIVLCEEGTKHKFQKVQLNYPSDDVTFALKESPDLPWLSKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1H1N6</accession>
    <name>I1H1N6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein arginine N-methyltransferase PRMT10</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100826165</name>
        <name evidence="11" type="ORF">BRADI_1g51130v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Ring-like homodimer.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557204.1">
        <property type="nucleotide sequence ID" value="XM_003557156.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H1N6"/>
    <dbReference type="STRING" id="15368.I1H1N6"/>
    <dbReference type="EnsemblPlants" id="KQK19897">
        <property type="protein sequence ID" value="KQK19897"/>
        <property type="gene ID" value="BRADI_1g51130v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK19898">
        <property type="protein sequence ID" value="KQK19898"/>
        <property type="gene ID" value="BRADI_1g51130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826165"/>
    <dbReference type="Gramene" id="KQK19897">
        <property type="protein sequence ID" value="KQK19897"/>
        <property type="gene ID" value="BRADI_1g51130v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19898">
        <property type="protein sequence ID" value="KQK19898"/>
        <property type="gene ID" value="BRADI_1g51130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826165"/>
    <dbReference type="eggNOG" id="KOG1499">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017375_1_2_1"/>
    <dbReference type="OMA" id="NSEPTHW"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044020">
        <property type="term" value="F:histone H4R3 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000012">
        <property type="entry name" value="Protein arginine N-methyltransferase PRMT10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000132">
        <property type="entry name" value="Protein arginine N-methyltransferase PRMT10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF68">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE PRMT10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="10">
        <location>
            <begin position="192"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK19897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="380" mass="42569" checksum="0609551E540DBCB6" modified="2012-06-13" version="1">MASLPNGAAAAAASSAAGGAPPVDKEVDFANYFCTYAYLYHQKEMLCDRVRMDAYHSAVFRNPHHFRGKVVLDVGTGSGILAIWSAQAGARKVYAVEATNVAEHARELVRANGVADIVEVIQGTMEDIVLPEKVDVIISEWMGYYLLRESMFDSVICARDRWLNPGGVMYPSHARMWLAPIRSGLGDKKMEDFEIAMNDWNLFVEDTDAYYGVNMNALTKAYRAEHEKFYLKSSIWNNLHPNQLIGQPAVIKEIDCLTATVDEIREVRAQVMLPISQERTRLAALAGWFDVHFRGSNQNPAVQEVELSTAPDENGGTHWGQQVFLLTPSLRVNEGDNVKVSFSMVRSKENHRLMDMDFTYELHESSGKKLPAVTTKIYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4I7</accession>
    <name>I1H4I7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tetraacyldisaccharide 4'-kinase</fullName>
            <ecNumber evidence="2">2.7.1.130</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100828121</name>
        <name evidence="10" type="ORF">BRADI_1g59740v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.130" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228484.1">
        <property type="nucleotide sequence ID" value="XM_010230182.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H4I7"/>
    <dbReference type="STRING" id="15368.I1H4I7"/>
    <dbReference type="EnsemblPlants" id="KQK21258">
        <property type="protein sequence ID" value="KQK21258"/>
        <property type="gene ID" value="BRADI_1g59740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828121"/>
    <dbReference type="Gramene" id="KQK21258">
        <property type="protein sequence ID" value="KQK21258"/>
        <property type="gene ID" value="BRADI_1g59740v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828121"/>
    <dbReference type="eggNOG" id="ENOG502QRUA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038816_4_0_1"/>
    <dbReference type="OMA" id="RAFPDHH"/>
    <dbReference type="OrthoDB" id="10266567at2759"/>
    <dbReference type="UniPathway" id="UPA00359">
        <property type="reaction ID" value="UER00482"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009029">
        <property type="term" value="F:lipid-A 4'-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001289">
        <property type="term" value="P:lipid X metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00409">
        <property type="entry name" value="LpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003758">
        <property type="entry name" value="LpxK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00682">
        <property type="entry name" value="lpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42724">
        <property type="entry name" value="TETRAACYLDISACCHARIDE 4'-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42724:SF1">
        <property type="entry name" value="TETRAACYLDISACCHARIDE 4'-KINASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02606">
        <property type="entry name" value="LpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK21258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="403" mass="44932" checksum="501F9AB43AC5D555" modified="2012-06-13" version="1">MATEKARQLLARLAVTPDAAVPDLPFLHRALLLPLLSAASVLLRLSVHSPLRPRLRQALLPVPVVSVGNLTWGGNGKTPMVDFLARSFHAMAISPLVLTRGYAGGDESKMLRRRLADTSAKIGVGANRAAVASSMLQKYGYVHPCEAFHPEKLPSACNRAVIDKSAKIGVAILDDGMQHRSLLRDVEIVMVNGLTPWGNTHFIPRGPMREPLSALTRADIVVIHNADLACEVQLETIQSTVQDSGATCPVFFSKLSPSHIFEVNQPWQRLPLNVLDGTIVLCISAIGCPDAFIHTVREIGPLKIDRLDFSDHHFFHDHDLKLIQERVRKLVDQHNKETIVIVTEKDYDRDPDALRMLDVKVWVLSSSLQIMPLKEQREDEFMRKVKDIITTRHGKSHRERAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1H699</accession>
    <name>I1H699_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100836996</name>
        <name evidence="2" type="ORF">BRADI_1g64620v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558091.1">
        <property type="nucleotide sequence ID" value="XM_003558043.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H699"/>
    <dbReference type="FunCoup" id="I1H699">
        <property type="interactions" value="1820"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H699"/>
    <dbReference type="EnsemblPlants" id="KQK22024">
        <property type="protein sequence ID" value="KQK22024"/>
        <property type="gene ID" value="BRADI_1g64620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836996"/>
    <dbReference type="Gramene" id="KQK22024">
        <property type="protein sequence ID" value="KQK22024"/>
        <property type="gene ID" value="BRADI_1g64620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836996"/>
    <dbReference type="eggNOG" id="ENOG502S3ZE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082558_1_0_1"/>
    <dbReference type="OMA" id="KTPHWLF"/>
    <dbReference type="OrthoDB" id="1921976at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33052">
        <property type="entry name" value="DUF4228 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="193" mass="21150" checksum="625A1A36CEE565A9" modified="2012-06-13" version="1">MGNLVSAAGAKSGGGKVVMADGSVRALSEPVSVAELMMDHPRHFVVDAHALRQQEKHHQQQRKAATKVAPLPADHVLGAGGVYVLLPAVRGKVSADEARRVLASASLARSRSMPGELRRKLSFRKSREEAGDERSAPLAEVDRTEETEAISAEGFEELRPEFLSRELSIRGWKPSLNTIEERVMPKKVSHWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCR2</accession>
    <name>I1HCR2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF295 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100829685</name>
        <name evidence="3" type="ORF">BRADI_2g05330v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564464.1">
        <property type="nucleotide sequence ID" value="XM_003564416.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230554.1">
        <property type="nucleotide sequence ID" value="XM_010232252.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314153.1">
        <property type="nucleotide sequence ID" value="XM_024458385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCR2"/>
    <dbReference type="STRING" id="15368.I1HCR2"/>
    <dbReference type="EnsemblPlants" id="KQK03069">
        <property type="protein sequence ID" value="KQK03069"/>
        <property type="gene ID" value="BRADI_2g05330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829685"/>
    <dbReference type="Gramene" id="KQK03069">
        <property type="protein sequence ID" value="KQK03069"/>
        <property type="gene ID" value="BRADI_2g05330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829685"/>
    <dbReference type="eggNOG" id="ENOG502R3DY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019286_1_2_1"/>
    <dbReference type="OMA" id="DYVIIWI"/>
    <dbReference type="OrthoDB" id="1023001at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050942">
        <property type="entry name" value="F-box_BR-signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44259:SF91">
        <property type="entry name" value="OS01G0183800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44259">
        <property type="entry name" value="OS07G0183000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="19"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="2">
        <location>
            <begin position="78"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK03069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41594" checksum="537189BC090262B5" modified="2012-06-13" version="1">MSSKESRLMELSSVRLADWANLQTDLVGLIIKKLGIPDFVKFRAVCTSWNRVCREVSNHPRVDPWLMLPTDLLDGAKFFSIPEKINQTIRIPSTATIFGSMWIPVGSSHGWLIFFSPTQGTIQLVNPISSAQIQLPPIGTRRFSKAILLDMSESNFTVAVIYGNHKGYKVARKGSKSWSFVDSKHILVDVFKHRRQLYTIDIYGTVEVWAEPPRAWQDEDAPQVDPHVHHNLVHYPQQKFNCLVETPAGDLVRVKRQSQNKFALWVLDKETLSFEKTTDIGEFGLFVSHYSSFCFPAKDHPNLKVNCIYFIDGYNNLCAFNLEHGTKELVEALETAHANNQQAPHLHRHQPLQEPFLWFTPSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HEA1</accession>
    <name>I1HEA1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100823973</name>
        <name evidence="4" type="ORF">BRADI_2g09930v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565630.1">
        <property type="nucleotide sequence ID" value="XM_003565582.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HEA1"/>
    <dbReference type="STRING" id="15368.I1HEA1"/>
    <dbReference type="EnsemblPlants" id="KQK03806">
        <property type="protein sequence ID" value="KQK03806"/>
        <property type="gene ID" value="BRADI_2g09930v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823973"/>
    <dbReference type="Gramene" id="KQK03806">
        <property type="protein sequence ID" value="KQK03806"/>
        <property type="gene ID" value="BRADI_2g09930v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823973"/>
    <dbReference type="eggNOG" id="ENOG502SQ4G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_570383_0_0_1"/>
    <dbReference type="OMA" id="HIGYGVY"/>
    <dbReference type="OrthoDB" id="673865at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HEA1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF65">
        <property type="entry name" value="FBD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="At1g61320/AtMIF1 LRR" evidence="3">
        <location>
            <begin position="127"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="FBD" evidence="2">
        <location>
            <begin position="419"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK03806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="520" mass="57868" checksum="2873A25DDA4D8436" modified="2012-06-13" version="1">MAAGAAEDPQDRLSGLPDDVLHSIIGRVPFKQAVRTNALSRRWPRLWLHALAASGVLDFTDRGFVRSQSRAHIVATVNRCLRVRGGGAPIDVLRVSLCPFGAFERDVVGWIAAALRRGAREVDVNLTQGRVELGAAFVNCRTELELPGDLFCAESSLARLSLGRCSLSNVPPGAPGLAGLTSLSLTHVDITDDAVRDTVACCRLLEFLSLRNCHLLKFVRIAGENLRGLEVVGCLDVRQLQVAAPALESFAFHGDILYFKYREDESLEFEPVEFIGKGNNTQTPSEATPLLRDAYLSHIGFGVYDDPLHEFAFSNLMEKVAHAKILTICSVGLLHIEETCNFYELMIDTPNLQELQLLMDSMDDDDVTRISGFFHPTPPPLLERLFIRLPAAACEHTNSGGATAIGNDEDIVLDFEIALDHLTFIKLVNFRGTRREMRLLRFVLRRAPILEHVVLLTPEEEGRALRPGDDDQNQQLLKIVQEHVSEIGKAFLWREAHVVVCRPREDCSRSPAHTKYYHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1HEP7</accession>
    <name>I1HEP7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100846191</name>
        <name evidence="2" type="ORF">BRADI_2g11160v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565684.1">
        <property type="nucleotide sequence ID" value="XM_003565636.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HEP7"/>
    <dbReference type="STRING" id="15368.I1HEP7"/>
    <dbReference type="EnsemblPlants" id="KQK04006">
        <property type="protein sequence ID" value="KQK04006"/>
        <property type="gene ID" value="BRADI_2g11160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846191"/>
    <dbReference type="Gramene" id="KQK04006">
        <property type="protein sequence ID" value="KQK04006"/>
        <property type="gene ID" value="BRADI_2g11160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846191"/>
    <dbReference type="eggNOG" id="ENOG502S7JI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146298_0_0_1"/>
    <dbReference type="OMA" id="HEEVMAC"/>
    <dbReference type="OrthoDB" id="1858060at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33181">
        <property type="entry name" value="OS01G0778500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33181:SF10">
        <property type="entry name" value="SSRA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK04006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="84" mass="9507" checksum="EC9AC0A35A7647EA" modified="2012-06-13" version="1">MGGSSWVRTMTSPFRKACTIFGPPPHKEGKKQPQQQQPTSAMMHHIVEAERAKLHCEVMACAYEDVQVMWSMLDKAKIQEFNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HG22</accession>
    <name>I1HG22_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Water stress and hypersensitive response domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100827152</name>
        <name evidence="3" type="ORF">BRADI_2g15410v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LEA type 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567827.1">
        <property type="nucleotide sequence ID" value="XM_003567779.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HG22"/>
    <dbReference type="STRING" id="15368.I1HG22"/>
    <dbReference type="EnsemblPlants" id="KQK04706">
        <property type="protein sequence ID" value="KQK04706"/>
        <property type="gene ID" value="BRADI_2g15410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827152"/>
    <dbReference type="Gramene" id="KQK04706">
        <property type="protein sequence ID" value="KQK04706"/>
        <property type="gene ID" value="BRADI_2g15410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827152"/>
    <dbReference type="eggNOG" id="ENOG502RZ6Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118295_0_0_1"/>
    <dbReference type="OMA" id="KGLSREC"/>
    <dbReference type="OrthoDB" id="588983at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009269">
        <property type="term" value="P:response to desiccation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1820:FF:000001">
        <property type="entry name" value="Desiccation protectant protein Lea14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045043">
        <property type="entry name" value="Lea14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013990">
        <property type="entry name" value="WHy-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31459">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31459:SF29">
        <property type="entry name" value="LEA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00769">
        <property type="entry name" value="WHy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Water stress and hypersensitive response" evidence="2">
        <location>
            <begin position="24"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK04706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="169" mass="18358" checksum="8286BAFB8E0F36FC" modified="2012-06-13" version="1">MASLIDKAKGFVADKIAHMPKPEASLDSVSFKGVSREALTVHSNINVTNPYSHRIPICDITFTLKCGGKVVASGTIPDPGWIEEGGEVTKLEVPAKVPYDFLISIMKDLGRDWDIDYELQVGLTIDLPIIGNFTIPLSTAGELKMPTFSDFFGGGGKDDEDDKKAKDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HI69</accession>
    <name>I1HI69_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100837869</name>
        <name evidence="5" type="ORF">BRADI_2g21470v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231232.1">
        <property type="nucleotide sequence ID" value="XM_010232930.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HI69"/>
    <dbReference type="STRING" id="15368.I1HI69"/>
    <dbReference type="EnsemblPlants" id="KQK05658">
        <property type="protein sequence ID" value="KQK05658"/>
        <property type="gene ID" value="BRADI_2g21470v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837869"/>
    <dbReference type="Gramene" id="KQK05658">
        <property type="protein sequence ID" value="KQK05658"/>
        <property type="gene ID" value="BRADI_2g21470v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837869"/>
    <dbReference type="eggNOG" id="KOG0027">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_2_0_1"/>
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    <dbReference type="OrthoDB" id="26525at2759"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="term" value="F:enzyme regulator activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Myosin 1 light chain cam2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Probable calcium-binding protein CML9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
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    </dbReference>
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        <property type="entry name" value="CALM/Myosin/TropC-like"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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    <dbReference type="PANTHER" id="PTHR23048:SF51">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK05658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="151" mass="16136" checksum="BF94BE237EBA407D" modified="2012-06-13" version="1">MAGKLTQAQADECREVFDLFDGDEDDRIAAGELVTALRSLGQNVDEAEARQFLEDAGAGSGGSGSIDFATFLAVAARKAGAGVSEKGLATWLDAFDDAQSGAIPAEQLRQVMVTHGDRLTEEEADEMLRKADPRGEGRVLCKEFVKVLMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HKM4</accession>
    <name>I1HKM4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sphingomyelin synthase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100828690</name>
        <name evidence="12" type="ORF">BRADI_2g31130v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingomyelin synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568684.1">
        <property type="nucleotide sequence ID" value="XM_003568636.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HKM4"/>
    <dbReference type="FunCoup" id="I1HKM4">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK06896">
        <property type="protein sequence ID" value="KQK06896"/>
        <property type="gene ID" value="BRADI_2g31130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828690"/>
    <dbReference type="Gramene" id="KQK06896">
        <property type="protein sequence ID" value="KQK06896"/>
        <property type="gene ID" value="BRADI_2g31130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828690"/>
    <dbReference type="eggNOG" id="KOG3058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078641_1_0_1"/>
    <dbReference type="OMA" id="NIRFIKM"/>
    <dbReference type="OrthoDB" id="422827at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HKM4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047493">
        <property type="term" value="F:ceramide cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045140">
        <property type="term" value="F:inositol phosphoceramide synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033188">
        <property type="term" value="F:sphingomyelin synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045221">
        <property type="entry name" value="Sphingomyelin_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290:SF62">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL:CERAMIDE INOSITOLPHOSPHOTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290">
        <property type="entry name" value="SPHINGOMYELIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingomyelin synthase-like" evidence="11">
        <location>
            <begin position="173"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK06896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="311" mass="35398" checksum="7EEE7D7ED8CB9744" modified="2012-06-13" version="1">MTPFYLARSASKVLRRITSETSVELKILSQKWRLLLAGVLFQYIHGLAARGVHYLHRPGPTLQDLGFMVLPELGKERGYISETLFTFIFLSFVLWTFHPFILQTKRFYTVLIWRRVLAFLCASQFLRIVTFYSTQLPGPNYHCREGSPLARLPPPQNVAEVLLINFPRGVIYGCGDLIFSSHMIFTLVFVITYQKYGSIRFIKVLAWCIAVAQSLLIVASRKHYSVDVVVAWYTVNLLVFFVDKKLTELPDRSAVSASALPLSVKDIKLKEESTRLLNGNSVEAADRRPRTQINGKPLENGNHLDTETTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HL79</accession>
    <name>I1HL79_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC104582731</name>
        <name evidence="6" type="ORF">BRADI_2g33676v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the heat shock protein 70 (TC 1.A.33) family. HSP110/SSE subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231644.1">
        <property type="nucleotide sequence ID" value="XM_010233342.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315949.1">
        <property type="nucleotide sequence ID" value="XM_024460181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HL79"/>
    <dbReference type="STRING" id="15368.I1HL79"/>
    <dbReference type="EnsemblPlants" id="KQK07192">
        <property type="protein sequence ID" value="KQK07192"/>
        <property type="gene ID" value="BRADI_2g33676v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104582731"/>
    <dbReference type="Gramene" id="KQK07192">
        <property type="protein sequence ID" value="KQK07192"/>
        <property type="gene ID" value="BRADI_2g33676v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104582731"/>
    <dbReference type="eggNOG" id="KOG0103">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_5_1_1"/>
    <dbReference type="OMA" id="WEQSPEI"/>
    <dbReference type="OrthoDB" id="434160at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HL79">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd24095">
        <property type="entry name" value="ASKHA_NBD_HSP70_AtHsp70-14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000171">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000018">
        <property type="entry name" value="Heat shock 70 kDa protein 4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF4">
        <property type="entry name" value="HSC70CB, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="497"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="771"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="547"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="777"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="791"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="827"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK07192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="836" mass="92728" checksum="BA7111EE89D7B2DC" modified="2012-06-13" version="1">MSVVGFDLGNESCIVGVARQRGIDVVLNEESKRETPAIVCFGDKQRFIGTAGAANSTMNPKNSISQIKRLLGRKFTDPEVQHDIQSFPFRVSEGPDGFPLVHARYLGEERTFTPTQLLAMVLSNLKGIAEGNLNTAVSDCCIGIPVYFTDLQRRAVLDAATIAGLRPLRLFHETTATALAYGIYKTDLPENDQLNVAFVDIGHASMQVSIVGYKKGQLKMLSHAYDRSLGGRDFDEALFKHFAAKFKEEYKIDVYQNARACIRLRVACEKLKKMLSANPEAPMNIECLMDEKDVRGFIKRDEFEQISGPVLERVKGPLDKALAEAGLTTESVHFVEVVGSGSRVPAIMKIITEFFGKEPRRTMNASECVARGCALQCAILSPTFKVREFQVNEGFPFSVALSWKPDSPGNEAQQTVVFPKGNPIPSIKALTFYRSNTFAVDVLNVDTDDMQITQKISTYTIGPFQSSKGDKAKLKVKVRLNIHGIVSLESATMLEEEEVEVPVTSEVPKDATKMDTDDATGTDVNMQESKGATDTAEGAAENGAQDSEEKSVPMDTDTKVEPSKKKVKKTNVPVTELVYGAMSAAELEKAVEKEYEMALQDRVMEETKDKKNSVEAYVYDMRNKLSEKYNDFVMSEDMEGLRVKLQEVEDWLYEDGEDETKGVYVAKLEELKKVGDPIEMRYKEWAERGQALEQLVYCIRSFREAALSSDQKFDHIDIAEKQKVVNECSNAETWLLEKKQEQDALPKHINPVLLVSDIKKKAEALDRFCKPIMTKPKPAPKPQTPPPAENPAPEAQTPEQQSSGASEADEPANEGASQDQPAAEQMETDRAEPSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1HS78</accession>
    <name>I1HS78_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100845063</name>
        <name evidence="9" type="ORF">BRADI_2g51630v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564345.1">
        <property type="nucleotide sequence ID" value="XM_003564297.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HS78"/>
    <dbReference type="EnsemblPlants" id="KQK10036">
        <property type="protein sequence ID" value="KQK10036"/>
        <property type="gene ID" value="BRADI_2g51630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845063"/>
    <dbReference type="Gramene" id="KQK10036">
        <property type="protein sequence ID" value="KQK10036"/>
        <property type="gene ID" value="BRADI_2g51630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845063"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005738_7_0_1"/>
    <dbReference type="OMA" id="PTWILIV"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000018">
        <property type="entry name" value="Aspartic proteinase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000020">
        <property type="entry name" value="Aspartic proteinase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF875">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094815" description="Peptidase A1 domain-containing protein" evidence="7">
        <location>
            <begin position="26"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="8">
        <location>
            <begin position="89"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK10036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="506" mass="53334" checksum="06D3D39FA7D6C190" modified="2012-06-13" version="1">MRPPEVSLAAAFVAVAALLAGAAAAAPFPAALTLERALPHKGVPVEHLKERDGAHHARRRGLLGGAPAVAGVVDFPVEGSANPYMVGLYFTRVKLGNPAKEYFVQIDTGSDILWVACSPCTGCPTSSGLNIQLEFFNPDSSSTSSRIPCSDDRCTAALQTGEAVCQSSDSPSSPCGYTFTYGDGSGTSGFYVSDTMYFDTVMGNEQTANSSASVVFGCSNSQSGDLMKTDRAVDGIFGFGQHQLSVVSQLYSLGVSPKTFSHCLKGSDNGGGILVLGEIVEPGLVFTPLVPSQPHYNLNLESIAVSGQKLPIDSSLFATSNTQGTIVDSGTTLVYLVDGAYDPFINAIAAAVSPSVRSVVSKGIQCFVTTSSVDSSFPTATLYFKGGVSMTVKPENYLLQQGSVDNNVLWCIGWQRSQGITILGDLVLKDKIFVYDLANMRMGWADYDCSLSVNVTSSSGKNQYVNTGQFDVNGSPLPLYRSCLVPTGVAVILVHMLIFGMLPSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HTG8</accession>
    <name>I1HTG8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Coronatine-insensitive protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100829997</name>
        <name evidence="3" type="ORF">BRADI_2g55210v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564629.1">
        <property type="nucleotide sequence ID" value="XM_003564581.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HTG8"/>
    <dbReference type="FunCoup" id="I1HTG8">
        <property type="interactions" value="641"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HTG8"/>
    <dbReference type="EnsemblPlants" id="KQK10625">
        <property type="protein sequence ID" value="KQK10625"/>
        <property type="gene ID" value="BRADI_2g55210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829997"/>
    <dbReference type="Gramene" id="KQK10625">
        <property type="protein sequence ID" value="KQK10625"/>
        <property type="gene ID" value="BRADI_2g55210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829997"/>
    <dbReference type="eggNOG" id="KOG1947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022456_1_0_1"/>
    <dbReference type="OMA" id="CYTATPD"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002213">
        <property type="term" value="P:defense response to insect"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009753">
        <property type="term" value="P:response to jasmonic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22159">
        <property type="entry name" value="F-box_AtTIR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000124">
        <property type="entry name" value="Coronatine-insensitive protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000034">
        <property type="entry name" value="Coronatine-insensitive protein homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041567">
        <property type="entry name" value="COI1_F-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041101">
        <property type="entry name" value="Transp_inhibit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134:SF43">
        <property type="entry name" value="CORONATINE-INSENSITIVE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134">
        <property type="entry name" value="F-BOX/TPR REPEAT PROTEIN POF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18511">
        <property type="entry name" value="F-box_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18791">
        <property type="entry name" value="Transp_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COI1 F-box" evidence="1">
        <location>
            <begin position="19"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Transport inhibitor response 1" evidence="2">
        <location>
            <begin position="78"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK10625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="594" mass="66420" checksum="1A0ACC2ABAB582C3" modified="2012-06-13" version="1">MGGEVPEPRRLHRALSFGCGVPDEALHLVMGYVDGPRDREAASLVCRRWHRIDALTRKHVTVAFCYAAEPSRLRARFPRLESLALKGKPRAAMYGLIPDDWGAYAAPWVAELAVPLDCLKALHLRRMTVTDADLAALVRARGHMLLELKLDKCSGFSTDALRLVARSCRSLRTLFLEECFITDKGDEWLHELAANNSVLVTLNFYMTELKVAPADLELLAKNCKSLLSLKMSECDLSDLIGFFQRANALEDFAGGSFYEVGELTKYEKVNFPPRVCLLGLTYMGKNEIPVIFPFSARLKKLDLQYTFLTAEDHCQVISKCPNLFVLEVRNVIGDRGLVVVGDTCKKLRRLRIERGDDDPGLQEEQGGVSQLGLTAVAVGCRDLEYIAAYVSDITNGALESIGTFCKNLYDFRLVLLDRQKEVTDLPLDNGVRALLRSCTKLRRFALYLRPGGLSDMGLEYIGQYSGNIQYMLLGNVGESDHGLVRFAMGCTNLRKLELRSSCFSERALSLAVLQMPSLRYIWVQGYRASPTGVDLLLMARPFWNIEFTPPDIVRHVTADGELCVDRQPQVLAYYSLAGKRPDCPGWVIPLHPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUD7</accession>
    <name>I1HUD7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100827868</name>
        <name evidence="11" type="ORF">BRADI_2g58120v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564878.1">
        <property type="nucleotide sequence ID" value="XM_003564830.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUD7"/>
    <dbReference type="FunCoup" id="I1HUD7">
        <property type="interactions" value="435"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HUD7"/>
    <dbReference type="MEROPS" id="C01.024"/>
    <dbReference type="EnsemblPlants" id="KQK11105">
        <property type="protein sequence ID" value="KQK11105"/>
        <property type="gene ID" value="BRADI_2g58120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827868"/>
    <dbReference type="Gramene" id="KQK11105">
        <property type="protein sequence ID" value="KQK11105"/>
        <property type="gene ID" value="BRADI_2g58120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827868"/>
    <dbReference type="eggNOG" id="KOG1543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012184_1_0_1"/>
    <dbReference type="OMA" id="EKGNHWI"/>
    <dbReference type="OrthoDB" id="10253408at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000326">
        <property type="term" value="C:protein storage vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000023">
        <property type="entry name" value="Senescence-specific cysteine protease SAG39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982785" description="Peptidase C1A papain C-terminal domain-containing protein" evidence="8">
        <location>
            <begin position="25"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="10">
        <location>
            <begin position="42"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="9">
        <location>
            <begin position="136"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK11105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="40814" checksum="DEADF9B3C572B4C3" modified="2012-06-13" version="1">MGLLSKKLLVACLALLAVVSLCSAIPFDAKDLESEEALWELYTRWQSAHRLPPQHHAEKHRRFGTFKSNVLFIHAHNTRLNDTSTNNNGPSYRLRLNRFGDMDQAEFRSTFAGPLHRHTRPAQSIPGFIYDTVKDIPQAVDWRQKGAVTGVKDQGKCGSCWAFSAVASVEGLNAIRTGSLVSLSEQELIDCDTGGDDNGCQGGLMESAFEFIAHSAGGLATEAAYPYHASNGTCNANRGSSVSVRIDGHQSVPAGNEEALAKAVAHQPVSVAIDAGGQAFQFYSEGVFTGDCGSELDHGVAVVGYGVAEEDGKEYWIVKNSWGPGWGEHGYVRMQRDSGVDGGLCGIAMEASYPVKNEQTKKKPRRALGEKKAYYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1HVM5</accession>
    <name>I1HVM5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100834697</name>
        <name evidence="8" type="ORF">BRADI_2g62067v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565161.1">
        <property type="nucleotide sequence ID" value="XM_003565113.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HVM5"/>
    <dbReference type="STRING" id="15368.I1HVM5"/>
    <dbReference type="EnsemblPlants" id="PNT73669">
        <property type="protein sequence ID" value="PNT73669"/>
        <property type="gene ID" value="BRADI_2g62067v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834697"/>
    <dbReference type="Gramene" id="PNT73669">
        <property type="protein sequence ID" value="PNT73669"/>
        <property type="gene ID" value="BRADI_2g62067v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834697"/>
    <dbReference type="eggNOG" id="ENOG502RIEW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055357_1_0_1"/>
    <dbReference type="OMA" id="PQAYHHA"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044841">
        <property type="entry name" value="LUX/BOA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31442">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31442:SF21">
        <property type="entry name" value="TRANSCRIPTION FACTOR BOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="111"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="64"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT73669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="256" mass="26735" checksum="B4F44BBD421CEDC9" modified="2012-06-13" version="1">MGEEAGGGGGGEYARVSEWETGLPGSDELTPLSQPLVPPGLAAAFRIPPEPGRTLLDVHRASSATVSRLRSSSSSSSGGGGSFPTFPSGHGGAASDTGADSAAAASELEKTSKRPRMVWNPQLHKRFVDVVAHLGIKSAVPKTIMQLMNVEGLTRENVASHLQKYRLYVKRMQGLSNEGPSPSDHIFASTPVPPSLREPQVPSAAMAPMYHHHPMGGGGGGMTGGYYQPQQQHGGHAVYNGYGGGGGYSQYHHGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1HVT2</accession>
    <name>I1HVT2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100842942</name>
        <name evidence="3" type="ORF">BRADI_2g62480v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565185.1">
        <property type="nucleotide sequence ID" value="XM_003565137.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HVT2"/>
    <dbReference type="STRING" id="15368.I1HVT2"/>
    <dbReference type="EnsemblPlants" id="KQK11798">
        <property type="protein sequence ID" value="KQK11798"/>
        <property type="gene ID" value="BRADI_2g62480v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73692">
        <property type="protein sequence ID" value="PNT73692"/>
        <property type="gene ID" value="BRADI_2g62480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842942"/>
    <dbReference type="Gramene" id="KQK11798">
        <property type="protein sequence ID" value="KQK11798"/>
        <property type="gene ID" value="BRADI_2g62480v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73692">
        <property type="protein sequence ID" value="PNT73692"/>
        <property type="gene ID" value="BRADI_2g62480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842942"/>
    <dbReference type="eggNOG" id="ENOG502SC5G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170630_1_0_1"/>
    <dbReference type="OrthoDB" id="724715at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48183">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48183:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK11798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="76" mass="8732" checksum="781A764972C48ED1" modified="2012-06-13" version="1">MAALDLSKLVKEKKFWVASFLVAWAAALQGHMMWLQRQDAFKDKFGDPQAPNNKVLTETEKPPPKNEQLTEDAEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I1HWP7</accession>
    <name>I1HWP7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106866491</name>
        <name evidence="2" type="ORF">BRADI_3g02480v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756234.1">
        <property type="nucleotide sequence ID" value="XM_014900748.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HWP7"/>
    <dbReference type="EnsemblPlants" id="KQJ93055">
        <property type="protein sequence ID" value="KQJ93055"/>
        <property type="gene ID" value="BRADI_3g02480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106866491"/>
    <dbReference type="Gramene" id="KQJ93055">
        <property type="protein sequence ID" value="KQJ93055"/>
        <property type="gene ID" value="BRADI_3g02480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:106866491"/>
    <dbReference type="eggNOG" id="ENOG502R47V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1519916_0_0_1"/>
    <dbReference type="OrthoDB" id="737602at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045501">
        <property type="entry name" value="DUF6490"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46610">
        <property type="entry name" value="OS05G0181300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46610:SF10">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20100">
        <property type="entry name" value="DUF6490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ93055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="177" mass="18531" checksum="AA000F3B6E3798CE" modified="2012-06-13" version="1">MEGGDDGGIRVDGDSGIGLIGVDGVGIGVIGGDVGIDGGGGGGFSWTFAASLLAFLTFNCGMAVWTSRKDASIVAFVATSYLDLLLLFCCLWFYNRAAPGSPRRSRLKVSVWTLTTLLTFSYCYMVLGTAAGLTLQVALLVWLIPAATGVGASYAFFDDCRNNEQPLDRGIMLAPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1HX51</accession>
    <name>I1HX51_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100823693</name>
        <name evidence="2" type="ORF">BRADI_3g03670v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316623.1">
        <property type="nucleotide sequence ID" value="XM_024460855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HX51"/>
    <dbReference type="STRING" id="15368.I1HX51"/>
    <dbReference type="EnsemblPlants" id="KQJ93290">
        <property type="protein sequence ID" value="KQJ93290"/>
        <property type="gene ID" value="BRADI_3g03670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823693"/>
    <dbReference type="Gramene" id="KQJ93290">
        <property type="protein sequence ID" value="KQJ93290"/>
        <property type="gene ID" value="BRADI_3g03670v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S0KJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098106_2_0_1"/>
    <dbReference type="OMA" id="MCYSSSE"/>
    <dbReference type="OrthoDB" id="670661at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF422">
        <property type="entry name" value="OS02G0143400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ93290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="129" mass="14646" checksum="BB6405F4D3FA8F0B" modified="2012-06-13" version="1">MGEQGRASSNKIRDIVRLQQLLKKWKRLALAPKAGNGKHGSGGAADVPRGFFAVCVGEEMRRFVIPTEYLGHWAFEELLREAEEEFGFRHEGALRIPCDVEVFEGILRLVGRKKEATCYFSSEYEVLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYS7</accession>
    <name>I1HYS7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100836967</name>
        <name evidence="10" type="ORF">BRADI_3g08210v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571714.1">
        <property type="nucleotide sequence ID" value="XM_003571666.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYS7"/>
    <dbReference type="FunCoup" id="I1HYS7">
        <property type="interactions" value="1577"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HYS7"/>
    <dbReference type="EnsemblPlants" id="KQJ94051">
        <property type="protein sequence ID" value="KQJ94051"/>
        <property type="gene ID" value="BRADI_3g08210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836967"/>
    <dbReference type="Gramene" id="KQJ94051">
        <property type="protein sequence ID" value="KQJ94051"/>
        <property type="gene ID" value="BRADI_3g08210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836967"/>
    <dbReference type="eggNOG" id="ENOG502RY7Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066104_2_1_1"/>
    <dbReference type="OMA" id="FSMFEPP"/>
    <dbReference type="OrthoDB" id="755577at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006459">
        <property type="entry name" value="CASP/CASPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01569">
        <property type="entry name" value="A_tha_TIGR01569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573:SF30">
        <property type="entry name" value="CASP-LIKE PROTEIN 2C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573">
        <property type="entry name" value="CASP-LIKE PROTEIN 4A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="9">
        <location>
            <begin position="5"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ94051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="180" mass="19326" checksum="635933BD974B41B5" modified="2012-06-13" version="1">MATARFKVLEMALRACVVPLAVASLWEMATNKQADDTYGEISFSNLSGFKYLVFANAITAAYSVASILLSSIKSFARYDWLIFLLDQAAAYLLVTSCSAAAELVYLAREGDKEVSWGEVCSYFGRFCGRATVSVALQGAALLCFVVLSLGSAFRVFSKCDAPCRPGAAAGSKQPEGQFAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZ81</accession>
    <name>I1HZ81_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC104583983</name>
        <name evidence="10" type="ORF">BRADI_3g09390v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236345.1">
        <property type="nucleotide sequence ID" value="XM_010238043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZ81"/>
    <dbReference type="EnsemblPlants" id="KQJ94236">
        <property type="protein sequence ID" value="KQJ94236"/>
        <property type="gene ID" value="BRADI_3g09390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583983"/>
    <dbReference type="Gramene" id="KQJ94236">
        <property type="protein sequence ID" value="KQJ94236"/>
        <property type="gene ID" value="BRADI_3g09390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583983"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084614_1_0_1"/>
    <dbReference type="OMA" id="QRSHMPA"/>
    <dbReference type="OrthoDB" id="663606at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF625">
        <property type="entry name" value="OS02G0248240 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="156"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ94236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="211" mass="22617" checksum="B24EDFC1E3A6E461" modified="2012-06-13" version="1">MGCFGRVLLLAMVSIVCFGGASMFLSLIISESRGGRLSNCSVAVLAVVLLFWVIIAGGMYVNCCDMYFPLSTTLGRCFRGAVGWLLFLPCRCCARSSQRPEIESGSGSDLVVPGQGRHLVVLAREPPVGGGGARVATTDDIPTTYEQPEGGGASECAACLGEVEQGETVKRLPACLHVFHRRCIDLWLRDHSTCPVCRRNVLAPLLPEQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1I506</accession>
    <name>I1I506_BRADI</name>
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        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100829417</name>
        <name evidence="3" type="ORF">BRADI_3g29740v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234857.1">
        <property type="nucleotide sequence ID" value="XM_010236555.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I506"/>
    <dbReference type="FunCoup" id="I1I506">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I506"/>
    <dbReference type="EnsemblPlants" id="KQJ97257">
        <property type="protein sequence ID" value="KQJ97257"/>
        <property type="gene ID" value="BRADI_3g29740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829417"/>
    <dbReference type="Gramene" id="KQJ97257">
        <property type="protein sequence ID" value="KQJ97257"/>
        <property type="gene ID" value="BRADI_3g29740v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0154">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="EFERAFY"/>
    <dbReference type="OrthoDB" id="21470at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I506">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039146">
        <property type="entry name" value="GPANK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20923">
        <property type="entry name" value="BAT4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20923:SF1">
        <property type="entry name" value="G PATCH DOMAIN AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="6"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ97257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="114" mass="13055" checksum="43A5D5729FF3CD7E" modified="2012-06-13" version="1">MASIGSSNIGFQLLKKSGWKEGTGLGAQEQGRLEPVETRVKNNKRGLGSKEPKPKPLVEDEVEKAPKRPKQDTMSTKKAKLAAKRIRKMQEEEKRLQEKEFEIAFFREFWPDNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5L1</accession>
    <name>I1I5L1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutathione S-transferase</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100841559</name>
        <name evidence="7" type="ORF">BRADI_3g31750v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Tau family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574231.1">
        <property type="nucleotide sequence ID" value="XM_003574183.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5L1"/>
    <dbReference type="FunCoup" id="I1I5L1">
        <property type="interactions" value="260"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I5L1"/>
    <dbReference type="EnsemblPlants" id="KQJ97548">
        <property type="protein sequence ID" value="KQJ97548"/>
        <property type="gene ID" value="BRADI_3g31750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841559"/>
    <dbReference type="Gramene" id="KQJ97548">
        <property type="protein sequence ID" value="KQJ97548"/>
        <property type="gene ID" value="BRADI_3g31750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841559"/>
    <dbReference type="eggNOG" id="KOG0406">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_18_0_1"/>
    <dbReference type="OMA" id="AKHIDHE"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000044">
        <property type="entry name" value="Glutathione S-transferase GSTU6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000023">
        <property type="entry name" value="Probable glutathione S-transferase GSTU6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054416">
        <property type="entry name" value="GST_UstS-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF790">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22041">
        <property type="entry name" value="GST_C_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="92"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ97548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="222" mass="23967" checksum="E9F1F191FDECE4B4" modified="2012-06-13" version="1">MAGGGGDLKMLGMWASPPALRVRLALSLKGVTYEYVEEDLKNKSDLLLKSNPVHNKVPVLIHGGKPVCESLGILEYIDEAFAGVGPSLLSADPYERAVARFWGAFIDETLVKAMYQASWGTKEEKEEGKKKVTAAVETLEGALRECSKGKPFFGGDSAGYLDVVLGGLLAWVRAVDGMQGLKIFDPSTMPLLAAWADSFAALDEVEAVMPDVNRLIEFARAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1I623</accession>
    <name>I1I623_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100841655</name>
        <name evidence="3" type="ORF">BRADI_3g33070v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the QWRF family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574314.1">
        <property type="nucleotide sequence ID" value="XM_003574266.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I623"/>
    <dbReference type="STRING" id="15368.I1I623"/>
    <dbReference type="EnsemblPlants" id="KQJ97757">
        <property type="protein sequence ID" value="KQJ97757"/>
        <property type="gene ID" value="BRADI_3g33070v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67861">
        <property type="protein sequence ID" value="PNT67861"/>
        <property type="gene ID" value="BRADI_3g33070v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67862">
        <property type="protein sequence ID" value="PNT67862"/>
        <property type="gene ID" value="BRADI_3g33070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841655"/>
    <dbReference type="Gramene" id="KQJ97757">
        <property type="protein sequence ID" value="KQJ97757"/>
        <property type="gene ID" value="BRADI_3g33070v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67861">
        <property type="protein sequence ID" value="PNT67861"/>
        <property type="gene ID" value="BRADI_3g33070v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67862">
        <property type="protein sequence ID" value="PNT67862"/>
        <property type="gene ID" value="BRADI_3g33070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841655"/>
    <dbReference type="eggNOG" id="ENOG502QR1J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035946_0_0_1"/>
    <dbReference type="OMA" id="EYHRFRV"/>
    <dbReference type="OrthoDB" id="663033at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007573">
        <property type="entry name" value="QWRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31807">
        <property type="entry name" value="AUGMIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31807:SF27">
        <property type="entry name" value="QWRF MOTIF-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04484">
        <property type="entry name" value="QWRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ97757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="378" mass="40575" checksum="0D3793138FAF3952" modified="2012-06-13" version="1">MESGGGTPRPQPVSSRRLGRVQSAASRAGAGAFLYDGMRSAPIFSSATFARSLRKAASFGSSHNKKPSVIAGDVAPPRRALSSKDQNTDAAAVTLSPRRSLPEPGRQGNCWEPTRRRGRSTGASSTSPEYAAAGRGSLACALRETPAAAARKIKEAPTGEKEEDAHRARVLATRLLQWRFANARLEVAVSRATSSAENKLFYTWLRVAELRNIQAAKRIVAQRRRQKLKLARLLRPELPLLASWEPHAAPHADAVAGLASALSAACTSVPVTSGAQADMEAMRETISACVDTVTEIETNADTFYTTAGATSGTLGELAKTIREEVEGLEEAMRLARIVTRLQTQEVSLRANLVQAKQRRLVDGDMMLVPGITTSGWCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1I633</accession>
    <name>I1I633_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100826873</name>
        <name evidence="8" type="ORF">BRADI_3g33160v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the expansin family. Expansin B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234991.1">
        <property type="nucleotide sequence ID" value="XM_010236689.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I633"/>
    <dbReference type="STRING" id="15368.I1I633"/>
    <dbReference type="EnsemblPlants" id="KQJ97768">
        <property type="protein sequence ID" value="KQJ97768"/>
        <property type="gene ID" value="BRADI_3g33160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826873"/>
    <dbReference type="Gramene" id="KQJ97768">
        <property type="protein sequence ID" value="KQJ97768"/>
        <property type="gene ID" value="BRADI_3g33160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826873"/>
    <dbReference type="eggNOG" id="ENOG502R4J6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027462_1_0_1"/>
    <dbReference type="OMA" id="HRMQAPF"/>
    <dbReference type="OrthoDB" id="613806at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22275">
        <property type="entry name" value="DPBB_EXPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005795">
        <property type="entry name" value="LolPI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF9">
        <property type="entry name" value="EXPANSIN-B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00829">
        <property type="entry name" value="LOLP1ALLERGN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095137" evidence="5">
        <location>
            <begin position="24"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="6">
        <location>
            <begin position="73"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="7">
        <location>
            <begin position="192"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ97768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="284" mass="31163" checksum="F3C99075F035E476" modified="2012-06-13" version="1">MGTLSSFAALAALLAVLAADAGAVEFNVTDATPADYGGAGLNSSEAAVYWGPWTPARATWYGQPNGAGPDDNGGACGFKHTNQYPFASMTSCGNQPLFKDGKGCGSCYKIRCRKDQSCSGRTETVIITDMNYYPVAPFHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVYFAVLVEYEDGDGDVVQVDLMESKTAHGPLTGQWTRMRESWGSIWRLDSNHRLQAPFSIRITNESGKRLIAREVIPANWRPNTFYRSFVQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1I670</accession>
    <name>I1I670_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF632 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100823081</name>
        <name evidence="4" type="ORF">BRADI_3g33490v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574338.1">
        <property type="nucleotide sequence ID" value="XM_003574290.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I670"/>
    <dbReference type="FunCoup" id="I1I670">
        <property type="interactions" value="1336"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I670"/>
    <dbReference type="EnsemblPlants" id="KQJ97818">
        <property type="protein sequence ID" value="KQJ97818"/>
        <property type="gene ID" value="BRADI_3g33490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823081"/>
    <dbReference type="Gramene" id="KQJ97818">
        <property type="protein sequence ID" value="KQJ97818"/>
        <property type="gene ID" value="BRADI_3g33490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823081"/>
    <dbReference type="eggNOG" id="ENOG502QU01">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010985_5_0_1"/>
    <dbReference type="OMA" id="FAFCDEW"/>
    <dbReference type="OrthoDB" id="1919226at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006868">
        <property type="entry name" value="DUF630"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006867">
        <property type="entry name" value="DUF632"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21450:SF9">
        <property type="entry name" value="BZIP DOMAIN CLASS TRANSCRIPTION FACTOR (DUF630 AND DUF632)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21450">
        <property type="entry name" value="PROTEIN ALTERED PHOSPHATE STARVATION RESPONSE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04783">
        <property type="entry name" value="DUF630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04782">
        <property type="entry name" value="DUF632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF630" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="DUF632" evidence="2">
        <location>
            <begin position="341"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ97818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="784" mass="87228" checksum="2C1D3EA140957431" modified="2012-06-13" version="1">MGCAASKVEQEDTVRRCKERRRNIRDAVAARQQLASAHADYLRSLRLTAAALSRFAQGHPSLAVSHHTAPVLLSTAAPPAALAPAPPAPAISSSVAPSSSLPPPTPPLPRHQAHHHPPPPPQPPQQQHAAAVSARERDPRMMRRLKVPHILSDSSVASPAQSSFRKPPVVGTPSSSSAWDWENFYPPSPPDSEFFDRRKTDLEEANRLRELDEEVDKARTRSYLHPGSRDRLKEEDEDEVATDDEEDDEDAHCGGWEDEEDHYASTTTSETRSEEGELGNRSECGFAARSEYGGTAPSEYAAAVPAPLQLRRRDERSEAGDSSSTVTAATEMRMVVRHRTLSEIVAAIEEYFVKAADAGDGVSELLEASRAQLDRNFRQLKKTVYHSNSVLSALSSTWTSKPPLAVRYKLDTNALEMESTEGKSHGSTLERLLAWEKKLYEEVKARESVKIEHEKKLSTLQSLEYRGRDSAKLDKTKASINKLQSLIVVTSQAATTTSTAIVRVRDTELAPQLVELCFALLSMWRSMNHFHEIQSEIVQQVRGLVDNSLAESTSDLHRLATRDLEAAVSAWYSNFNRLIKYQRDYIRSLYGWLKLTLFQVDSVAPQQAHASLISRELTSFCDEWKQALDRLPDAVASEAIKSFVNVVHVIYTKQAEEMKIKKRTETYAKELEKKANSLRAIEKKYYQSYSMVGLGLPGSGRDGIEGHSFDARDPLSEKKTEIAQCRRKVEDEITRHAKAVEVTRSMTLNNIQTGLPGMFQAIAAFSSTVAEALDVVCRRAGSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1I9V5</accession>
    <name>I1I9V5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100822142</name>
        <name evidence="13" type="ORF">BRADI_3g43890v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574998.1">
        <property type="nucleotide sequence ID" value="XM_003574950.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I9V5"/>
    <dbReference type="FunCoup" id="I1I9V5">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I9V5"/>
    <dbReference type="EnsemblPlants" id="KQJ99559">
        <property type="protein sequence ID" value="KQJ99559"/>
        <property type="gene ID" value="BRADI_3g43890v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822142"/>
    <dbReference type="Gramene" id="KQJ99559">
        <property type="protein sequence ID" value="KQJ99559"/>
        <property type="gene ID" value="BRADI_3g43890v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822142"/>
    <dbReference type="eggNOG" id="KOG0156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_4_0_1"/>
    <dbReference type="OMA" id="HRMILSY"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11075">
        <property type="entry name" value="CYP77_89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000012">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051103">
        <property type="entry name" value="Plant_metabolite_P450s"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298:SF80">
        <property type="entry name" value="OS02G0108800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ99559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="57161" checksum="72FE081C2A85DAE3" modified="2012-06-13" version="1">MELTIWPTLLLLITLSFSLTAALLFLLNGKNKQEQLPPGPPALLFLAKFLALRRSIFDLGPLLLELHARYGPVISVRLFRTLVFVSDRCLAHRVLVQSGSTFADRPRLFDPGLLFTSGSRNINAAPYGAYWRLVRRNLASEALHPARVSLFAPARRRMRDALVRDLLARGGGDGSRAVTVRTPFRNAMFELLVYMSLGASGLAPEVLDEIQELQLWVVRTITGFPFFSFFPALTKRLFRKRWAAHLAVRRRQDEIFLPFIEARRARVGAAANDEPPCYADFLLTLRVAEEGDRGLTDSELVSLCSEFLSGGTDSTMTLLEWIMAELVTHPEMQAKVYEEVKAKPELNESDLQVMPYLKAVVLEGLRLHPPAHFLLPHGVQSDRGAEIGGYRIPNGAEVNFLVAEFGRDEAVWAAAREFRPERFLDGGDGCGVDITGSREIRMMPFGAGRRMCPGYTLALLHLEFFVGSLVRELEWLPAAEGEAVDMTEMLDFTTLMKHPLRARTVPRTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1ID69</accession>
    <name>I1ID69_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100842967</name>
        <name evidence="9" type="ORF">BRADI_3g53270v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317637.1">
        <property type="nucleotide sequence ID" value="XM_024461869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ID69"/>
    <dbReference type="FunCoup" id="I1ID69">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1ID69"/>
    <dbReference type="EnsemblPlants" id="KQK01010">
        <property type="protein sequence ID" value="KQK01010"/>
        <property type="gene ID" value="BRADI_3g53270v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842967"/>
    <dbReference type="Gramene" id="KQK01010">
        <property type="protein sequence ID" value="KQK01010"/>
        <property type="gene ID" value="BRADI_3g53270v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842967"/>
    <dbReference type="eggNOG" id="KOG2744">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029075_2_1_1"/>
    <dbReference type="OMA" id="AMSHDGH"/>
    <dbReference type="OrthoDB" id="338531at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00298">
        <property type="entry name" value="ACD_sHsps_p23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000009">
        <property type="entry name" value="AT-rich interactive domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000014">
        <property type="entry name" value="AT-rich interactive domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045147">
        <property type="entry name" value="ARI3A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN ARID DOMAIN- CONTAINING PROTEIN DEAD RINGER PROTEIN B-CELL REGULATOR OF IGH TRANSCRIPTION BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348:SF4">
        <property type="entry name" value="OS02G0714300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ARID" evidence="8">
        <location>
            <begin position="175"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="SHSP" evidence="7">
        <location>
            <begin position="373"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK01010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="52339" checksum="422117F8EC5E0714" modified="2012-06-13" version="1">MSDSTDSDHDLTPADGANGDEAHQPEPLAVKEEEEEEVPMVADEFKDAPVSPETNSDGEGEDSPDRAERPNAGEGRKPAPPDAGGDEGEEVTVGVKVETNGEDAMSHDGDEEDGDDEDDDDDDEEEEEDDDDSTPDASPRAEVKAEGDSSTGLAPCSSQPVEPDPFLDGHDSGTEEEQTAFMAELERFHRDHNLEFKPPKFYGKGLNCLKLWRQVAHLGGHEQVTVCKLWRQVGETFRPPKTCTTVSWSFRIFYEKALLEYEKHKVRTGQFKLSLSALPQSGGIDREMGVNPSSSARVRRDAAACAMQGWHAHRLLSNGMYGDHMLKDKDSIPLPSRDKNLKGFGVLKRKKASSPERAFKVSRSKVNKSQEDSMVIDVGEPADWVKINVRQTKECFEIYALVPGLLREEVHVQSDPAGRLVITGDPDQPDNPWGITPFKKVINLPLRIDPHQTSAVVTLHGQLFVRAPFGHPDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDU6</accession>
    <name>I1IDU6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100843074</name>
        <name evidence="3" type="ORF">BRADI_3g55180v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572941.1">
        <property type="nucleotide sequence ID" value="XM_003572893.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDU6"/>
    <dbReference type="STRING" id="15368.I1IDU6"/>
    <dbReference type="EnsemblPlants" id="KQK01328">
        <property type="protein sequence ID" value="KQK01328"/>
        <property type="gene ID" value="BRADI_3g55180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843074"/>
    <dbReference type="Gramene" id="KQK01328">
        <property type="protein sequence ID" value="KQK01328"/>
        <property type="gene ID" value="BRADI_3g55180v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843074"/>
    <dbReference type="eggNOG" id="ENOG502QRJ2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_991738_0_0_1"/>
    <dbReference type="OMA" id="WADEAHK"/>
    <dbReference type="OrthoDB" id="1909968at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04051">
        <property type="entry name" value="C2_SRC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044750">
        <property type="entry name" value="C2_SRC2/BAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF143">
        <property type="entry name" value="CALCIUM-DEPENDENT LIPID-BINDING (CALB DOMAIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK01328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="278" mass="29915" checksum="2695DCFD4A7D64A6" modified="2012-06-13" version="1">MSAGGLDGCSPPPHLLEVTVISAQDLHRGRLGLGRRRVRAYAMAWTDGARKLRTGVDLAGGADPTWNDRFLFRVDPGFLRSETASVAVEVRGARSLLGGDAVLGHTRIVVSAFVSASRGDGRPIGGRQVAALQLRRPRSLRPQGIVNVAVALLDGATHAVPPVYNAPGSPDAFAVKDLMAVAARQLPPALGKIAEEDEEEGGAVLDHHGRRRRFVENSGPLDARGAAVEQSKLEMKLEKWKADLSPDHGEEGGRGGKSARRRRRRSSCFFGSEEDWER</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IH43</accession>
    <name>I1IH43_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metal transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100828548</name>
        <name evidence="8" type="ORF">BRADI_4g03490v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP (TC 2.A.55) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579243.1">
        <property type="nucleotide sequence ID" value="XM_003579195.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IH43"/>
    <dbReference type="FunCoup" id="I1IH43">
        <property type="interactions" value="1563"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IH43"/>
    <dbReference type="EnsemblPlants" id="KQJ86130">
        <property type="protein sequence ID" value="KQJ86130"/>
        <property type="gene ID" value="BRADI_4g03490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828548"/>
    <dbReference type="Gramene" id="KQJ86130">
        <property type="protein sequence ID" value="KQJ86130"/>
        <property type="gene ID" value="BRADI_4g03490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828548"/>
    <dbReference type="eggNOG" id="KOG1291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020088_5_1_1"/>
    <dbReference type="OMA" id="KMCFNYF"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006828">
        <property type="term" value="P:manganese ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF104">
        <property type="entry name" value="METAL TRANSPORTER NRAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="511"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ86130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="59431" checksum="B50ED3C9752AFB70" modified="2012-06-13" version="1">MSAGPGNGAGAAALPSDGAGAEDEARALLPASTPASDAAGGGGKGEEQEEDLEERAFEASEKVIVSLSDDPDGEDDDDLEGQLSTSSASPPFSWRKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAAAGDTLLWLLMWATAMGLLVQLLAARLGVATGRHLAELCRDEYPDWARRALWLMAEVAMVGADIQEVIGSAIAIKILSKGYLPLWAGVVITALDCFIFLSLENYGVRKLEAVFAVLIATMAVSFAWMFTDTKPNGKDLLIGILVPKLSSKTIRQAVGVVGCVIMPHNVFLHSALVQSRKVDPSKEYRVREALRYYSIESTIALAISFMINLFVTTVFAKGFYGTKEAGSIGLENAGQYLQEKFGGGFLPILYIWGIGLLAAGQSSTITGTYAGQFIMGGFLNLRLKKWIRSLITRSFAIVPTIIVALFFDSSDALDVLNEWLNVLQSIQIPFALIPLITLVSKEEVMGVFKIGRKMQAVTWTVAALLITINGYLLLDFFSSEVRGLLYGSLLCVAVLAYASFVLYLILRGTEMSNHIIVAIRKRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMH1</accession>
    <name>I1IMH1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g22005v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IMH1"/>
    <dbReference type="FunCoup" id="I1IMH1">
        <property type="interactions" value="493"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ88902">
        <property type="protein sequence ID" value="KQJ88902"/>
        <property type="gene ID" value="BRADI_4g22005v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88902">
        <property type="protein sequence ID" value="KQJ88902"/>
        <property type="gene ID" value="BRADI_4g22005v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2349636_0_0_1"/>
    <dbReference type="InParanoid" id="I1IMH1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IMH1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ88902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="99" mass="10138" checksum="A06E933F374E052D" modified="2012-06-13" version="1">MSAAVANPPGAYFVGHPTHPEEQKEPHPVDNQNAANVQASGTGAYYSGQPLGLNAADGANVQASRHDAGNLTEPSAAKKKRGSFAKLFSGCFSSGEVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1IP84</accession>
    <name>I1IP84_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100838837</name>
        <name evidence="7" type="ORF">BRADI_4g27620v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577958.1">
        <property type="nucleotide sequence ID" value="XM_003577910.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238039.1">
        <property type="nucleotide sequence ID" value="XM_010239737.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IP84"/>
    <dbReference type="STRING" id="15368.I1IP84"/>
    <dbReference type="EnsemblPlants" id="KQJ89767">
        <property type="protein sequence ID" value="KQJ89767"/>
        <property type="gene ID" value="BRADI_4g27620v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89768">
        <property type="protein sequence ID" value="KQJ89768"/>
        <property type="gene ID" value="BRADI_4g27620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838837"/>
    <dbReference type="Gramene" id="KQJ89767">
        <property type="protein sequence ID" value="KQJ89767"/>
        <property type="gene ID" value="BRADI_4g27620v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89768">
        <property type="protein sequence ID" value="KQJ89768"/>
        <property type="gene ID" value="BRADI_4g27620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838837"/>
    <dbReference type="eggNOG" id="ENOG502QU84">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053944_0_0_1"/>
    <dbReference type="OMA" id="TPDSACH"/>
    <dbReference type="OrthoDB" id="551907at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025756">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF79">
        <property type="entry name" value="HTH MYB-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="23"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="158"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ89767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="281" mass="30541" checksum="A435A9BE2748C80F" modified="2012-06-13" version="1">MELGGNNMGSNDGANSKASLAARQRLRWTDELHEQFVEAVTQLGGPDRATPKGVLRIMGTPGLTIYHVKSHLQKYRLAKYIPDSSTDGNKSDNKDPGDSLAGLDGSSGLQISEALKLQMEVQKRLHEQLEVQRQLQLRIEAQGKYLKKIIEEQQRYGGIKSETPGAGGTATVSSDQFPDSERTDPSTPAPTSESSQGVPFKRDNGGQTEATKSPCHDEQLTTDSNCHPGSPTVSPKHERAAKRQRGNGTEFSEADLSLPQHIFESSSGPEFQQCSVPYSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQB9</accession>
    <name>I1IQB9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100822379</name>
        <name evidence="14" type="ORF">BRADI_4g30940v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578152.1">
        <property type="nucleotide sequence ID" value="XM_003578104.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQB9"/>
    <dbReference type="FunCoup" id="I1IQB9">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ90345">
        <property type="protein sequence ID" value="KQJ90345"/>
        <property type="gene ID" value="BRADI_4g30940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822379"/>
    <dbReference type="Gramene" id="KQJ90345">
        <property type="protein sequence ID" value="KQJ90345"/>
        <property type="gene ID" value="BRADI_4g30940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822379"/>
    <dbReference type="eggNOG" id="KOG2287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051579_0_0_1"/>
    <dbReference type="OMA" id="MWHETAQ"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000037">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF122">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ90345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="322" mass="35744" checksum="6218670D5327F0A3" modified="2012-06-13" version="1">MKPTSSSSSFQASTLCTPYLLLVPLGLLAAVLVLPSLGSSHVRSDGLGVVLCPVLPATEAAASGAEKKADDASPTTAPEFRLLVGVLTTPARHERRDIVRLAYALQPPAPAYAHVDVRFVFCDVADPTERVLVSLEAARHGDVLILNCTENMNDGKTHEYFSSVPRLFAAAPYDYVMKTDDDTYLRVAAMAEELRPKPRRDVYLGHGFAVGDDPMPFMHGMGYVVSWDVAAWVSDNEDILRHNDTHGPEDLLFGKWLGIGGRGKNRYSLKPRMYDLNWYMDNFRPDTVAVHMLKDNRRWAAAFRYFNVTQEVNSSSTLYHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IRL4</accession>
    <name>I1IRL4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_4g34650v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Stipodae</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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        <source>
            <strain evidence="8 9">Bd21</strain>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Chromosome</location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <accession>I1ISI5</accession>
    <name>I1ISI5_BRADI</name>
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        </submittedName>
    </protein>
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ISI5"/>
    <dbReference type="STRING" id="15368.I1ISI5"/>
    <dbReference type="EnsemblPlants" id="KQJ91342">
        <property type="protein sequence ID" value="KQJ91342"/>
        <property type="gene ID" value="BRADI_4g37052v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_4g37052v3"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT 2"/>
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    </dbReference>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ91342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="263" mass="29677" checksum="BCC29F3D7202CE07" modified="2012-06-13" version="1">MIWHVQNENFILDSTRIFMKALHLLVFHGSFIFPECILIFGLILLLMIDSTSNQKDRPWFYFISSTSLVISIMALLFRSREEPIISFSGNFQTNNFNEIFQFLILLCSTLCIPLSVEYIECTEMAITEFLLFVLTATLGGMFLCGANDLITIFVASEYYSLCSYLLSGYTKRDLRSNEATMKYLLMGGASSSILVHGFSWLYGSSGGEIELQEIVNGLINTQMYNSPGISIALISITVGLGFKLSPAPFHQWTPDVYEGVWFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITX5</accession>
    <name>I1ITX5_BRADI</name>
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        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_4g41200v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
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            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1ITX5"/>
    <dbReference type="EnsemblPlants" id="KQJ92014">
        <property type="protein sequence ID" value="KQJ92014"/>
        <property type="gene ID" value="BRADI_4g41200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92014">
        <property type="protein sequence ID" value="KQJ92014"/>
        <property type="gene ID" value="BRADI_4g41200v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R3QU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048499_0_0_1"/>
    <dbReference type="InParanoid" id="I1ITX5"/>
    <dbReference type="OMA" id="IFHADMY"/>
    <dbReference type="OrthoDB" id="673859at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF10">
        <property type="entry name" value="OS12G0136300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="43"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="316"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="351"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="419"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ92014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="455" mass="49796" checksum="703CD785E521BA8C" modified="2012-06-13" version="1">MAIKNKNRKKRASNAASVAPPAMAVAIPPPPKPAANPLQPPPLPPGVYFSPTRDESVGILNRWIAGEPLHDTLGFVFVEDIYGSGPEELRRRYPPACVRGRKHSWWFLSQTKFQSSKQPPSGVRRRADRRVATGGYWRLEQSRKEIPVAGGVKNCFGFYLGPPGGGKRNKTPWLVEEFTSALDDGTGKGGVPALYRLYVTPRASNDDVRGIFGEDGIECVRGVKRPMRVVVPAHLFDAVAGLLPPGRVRYRAPAQQHPPPPPPPPPAALLDYEQQGQFFSGAGVLPGQYDYEQQFWGVAPPSEGVLLGQYDQQFWGFRPPPPPPPASPDLFHEVPAGDHGLYGEQFFSENAPPPPPPPPTSPGPFHEIPAGGLEQHDDQMFSGTAPPPPPPPESPGLYLEISGDNMSVTMDELLQLIDEKPAEEAEKPAEEAEADQEWDSFVTIVDDFERFSQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITZ5</accession>
    <name>I1ITZ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104584768</name>
        <name evidence="3" type="ORF">BRADI_4g41367v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238663.1">
        <property type="nucleotide sequence ID" value="XM_010240361.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238664.1">
        <property type="nucleotide sequence ID" value="XM_010240362.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITZ5"/>
    <dbReference type="EnsemblPlants" id="KQJ92035">
        <property type="protein sequence ID" value="KQJ92035"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92036">
        <property type="protein sequence ID" value="KQJ92036"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92037">
        <property type="protein sequence ID" value="KQJ92037"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92038">
        <property type="protein sequence ID" value="KQJ92038"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT65369">
        <property type="protein sequence ID" value="PNT65369"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT65370">
        <property type="protein sequence ID" value="PNT65370"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584768"/>
    <dbReference type="Gramene" id="KQJ92035">
        <property type="protein sequence ID" value="KQJ92035"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92036">
        <property type="protein sequence ID" value="KQJ92036"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92037">
        <property type="protein sequence ID" value="KQJ92037"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92038">
        <property type="protein sequence ID" value="KQJ92038"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65369">
        <property type="protein sequence ID" value="PNT65369"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65370">
        <property type="protein sequence ID" value="PNT65370"/>
        <property type="gene ID" value="BRADI_4g41367v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584768"/>
    <dbReference type="eggNOG" id="ENOG502RYTW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003068_2_0_1"/>
    <dbReference type="OMA" id="ANEWIRY"/>
    <dbReference type="OrthoDB" id="582804at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053197">
        <property type="entry name" value="F-box_SCFL_complex_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223:SF26">
        <property type="entry name" value="OS02G0188900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223">
        <property type="entry name" value="OS11G0201299 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="12"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="482" mass="55081" checksum="1554C27A9864166A" modified="2012-06-13" version="1">MADPPASRRRPVARINDLPDDILRNVLSFLSSRDAVRDCMLSRRWRDLWKSVPRIDADITDFDDQGYSDEQCEVLLKRFMNRFLEARDPVPLQEFCLWYSVCKDVPKTESEDASGDDEDAQFAEDDDEEAQFEEDDDEDAQFAEDDDEEAPIAEDDDEEAQLADAPEDDEEAQLADDEDEQDKSADVSEEDEQDKSAAANEWIRYALKHNASVIVVVNRLYALNLDHAGFTSNYLKSLNLSSAILMQGFFEQLQSGCPKLKHLSLYDCVIMDTEVSSQTLEVLTLPDQVHFPPDHQATISAPRLISLAFNNSEGPRLPLLRNMASLEEAWIQISENIKAIDAPDITQFLDSLSRVRRLSFNYDNRRLTMENNCQWCPTFSNLTDLTLDEWCVHEDFYALIVFLQKSPKLKDLTLILNEPHQRFGEEPAIIGALEERSFACNQLESVEIICPSECSLLPKVQTFLLDGGITLDKIRIKQKQHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IUJ3</accession>
    <name>I1IUJ3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100830988</name>
        <name evidence="11" type="ORF">BRADI_4g43090v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Type-A response regulators seem to act as negative regulators of the cytokinin signaling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARR family. Type-A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238744.1">
        <property type="nucleotide sequence ID" value="XM_010240442.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IUJ3"/>
    <dbReference type="STRING" id="15368.I1IUJ3"/>
    <dbReference type="EnsemblPlants" id="KQJ92353">
        <property type="protein sequence ID" value="KQJ92353"/>
        <property type="gene ID" value="BRADI_4g43090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830988"/>
    <dbReference type="Gramene" id="KQJ92353">
        <property type="protein sequence ID" value="KQJ92353"/>
        <property type="gene ID" value="BRADI_4g43090v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="LEQQEPM"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IUJ3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17581">
        <property type="entry name" value="REC_typeA_ARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF167">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="1 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="10">
        <location>
            <begin position="11"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="160"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="162"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="8">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ92353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="226" mass="24910" checksum="05EC3C586A3C35E0" modified="2012-06-13" version="1">MAAAIAETQFHVLAVDDSLPDRKLIERLLKTSSFQVTTVDSGTKALEFLGIHGQDTPISLHADHLQDVEVNVNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENVPSRINRCLEEGANEFFLKPVRLSDMSKLKPHIMKSRCKEHCHHEEEDLLSNSNSNNSCNPTNNSSSDSSNPTNNNTDSANNSSGDSGNTRKRKATEDEILPKTSRSKAQLAAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1IWK5</accession>
    <name>I1IWK5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC112269529</name>
        <name evidence="4" type="ORF">BRADI_5g04740v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312192.1">
        <property type="nucleotide sequence ID" value="XM_024456424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IWK5"/>
    <dbReference type="STRING" id="15368.I1IWK5"/>
    <dbReference type="EnsemblPlants" id="PNT60781">
        <property type="protein sequence ID" value="PNT60781"/>
        <property type="gene ID" value="BRADI_5g04740v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT60782">
        <property type="protein sequence ID" value="PNT60782"/>
        <property type="gene ID" value="BRADI_5g04740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112269529"/>
    <dbReference type="Gramene" id="PNT60781">
        <property type="protein sequence ID" value="PNT60781"/>
        <property type="gene ID" value="BRADI_5g04740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60782">
        <property type="protein sequence ID" value="PNT60782"/>
        <property type="gene ID" value="BRADI_5g04740v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R47K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101981_0_0_1"/>
    <dbReference type="OMA" id="EACHDIE"/>
    <dbReference type="OrthoDB" id="681126at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF428">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="118"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT60781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="281" mass="29487" checksum="3D5890EFE19AB336" modified="2012-06-13" version="1">MSAVAPPLAATAAAGCDPPAQKPQEEIITVNPKPDLTMEEDPAAGSYPVLVQITTASSSSLADGMPMLLMRSDDSLPGSLQGVQAPLQLVPATASFKDAGAAPTTQHQQQVKRESAARELRGWLMVLATVVASITYASGLSPPGGFQRLHAESSSAGAVAATVAPPPPHLGASFVDRMMPVLRDVSPTRYRTFYYSNTAAFALSLSIVLLLASHDLRKLAKLKALEALVGMDVLALLVAYIAGSTFSFVELVACVGLVLIVPLALVLMSSRLCGKFFWDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IYD0</accession>
    <name>I1IYD0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100841000</name>
        <name evidence="12" type="ORF">BRADI_5g12020v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581298.1">
        <property type="nucleotide sequence ID" value="XM_003581250.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IYD0"/>
    <dbReference type="EnsemblPlants" id="KQJ82915">
        <property type="protein sequence ID" value="KQJ82915"/>
        <property type="gene ID" value="BRADI_5g12020v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841000"/>
    <dbReference type="Gramene" id="KQJ82915">
        <property type="protein sequence ID" value="KQJ82915"/>
        <property type="gene ID" value="BRADI_5g12020v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841000"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="OMA" id="AYWLCYG"/>
    <dbReference type="OrthoDB" id="648285at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015145">
        <property type="term" value="F:monosaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17361">
        <property type="entry name" value="MFS_STP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000002">
        <property type="entry name" value="Sugar transport protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044778">
        <property type="entry name" value="MFS_STP/MST-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF92">
        <property type="entry name" value="OS04G0452600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="22"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ82915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="508" mass="54210" checksum="5420E753130BBA04" modified="2012-06-13" version="1">MAAGDGAASAYGGELTFSVIITCLVAASGGLIFGYDIGISGGVSQMKPFLETFFPKVLRRMADAKRSQYCMFDSHALTAFTSSLYIAGLVASLFAGRVTRSLGRRGVMLVGGALFFAGGIMTGAAVNLAMLIVGRMLLGFGVGFTNQATPLYLAEMAPAQWRGSLGVAFQFFLSLGILIANLVNYGTARVQWGWRVSLGLAGAPAVVMVVGAFFLTDTPSSYVMRGKADLARAALVRVRGRGGDVDAELKDITRAVEAARSSQKGGFRKLIGSREYRPHLTFALALPLCHQLSGMMVLTFFSPLVFRIAGFGSNAALMGAVILAGVKFVSLILSTLVIDRYGRKVLVIAGAAIMIVCQVANAWIMRAQGGKNGEVPLPRPYGLALLVLTCVQGAGFGMSWAPLIWVIPGEIFPMEIRSAGQSVSVSVVLGLTFLQTQTFLALLCAFKYATFVYYASWVAVMTAFVLVFLPETKGVPLESMGSVWEGHWYWRRFVGGDGHRVKASPSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZS8</accession>
    <name>I1IZS8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100824868</name>
        <name evidence="4" type="ORF">BRADI_5g16067v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240152.1">
        <property type="nucleotide sequence ID" value="XM_010241850.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240153.1">
        <property type="nucleotide sequence ID" value="XM_010241851.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311655.1">
        <property type="nucleotide sequence ID" value="XM_024455887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZS8"/>
    <dbReference type="STRING" id="15368.I1IZS8"/>
    <dbReference type="EnsemblPlants" id="KQJ83656">
        <property type="protein sequence ID" value="KQJ83656"/>
        <property type="gene ID" value="BRADI_5g16067v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83657">
        <property type="protein sequence ID" value="KQJ83657"/>
        <property type="gene ID" value="BRADI_5g16067v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61508">
        <property type="protein sequence ID" value="PNT61508"/>
        <property type="gene ID" value="BRADI_5g16067v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61509">
        <property type="protein sequence ID" value="PNT61509"/>
        <property type="gene ID" value="BRADI_5g16067v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61510">
        <property type="protein sequence ID" value="PNT61510"/>
        <property type="gene ID" value="BRADI_5g16067v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824868"/>
    <dbReference type="Gramene" id="KQJ83656">
        <property type="protein sequence ID" value="KQJ83656"/>
        <property type="gene ID" value="BRADI_5g16067v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83657">
        <property type="protein sequence ID" value="KQJ83657"/>
        <property type="gene ID" value="BRADI_5g16067v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61508">
        <property type="protein sequence ID" value="PNT61508"/>
        <property type="gene ID" value="BRADI_5g16067v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61509">
        <property type="protein sequence ID" value="PNT61509"/>
        <property type="gene ID" value="BRADI_5g16067v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61510">
        <property type="protein sequence ID" value="PNT61510"/>
        <property type="gene ID" value="BRADI_5g16067v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824868"/>
    <dbReference type="eggNOG" id="KOG2051">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002633_2_0_1"/>
    <dbReference type="OMA" id="ESVREYH"/>
    <dbReference type="OrthoDB" id="27832at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IZS8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000026">
        <property type="entry name" value="Regulator of nonsense transcripts UPF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000030">
        <property type="entry name" value="Regulator of nonsense transcripts UPF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000033">
        <property type="entry name" value="Regulator of nonsense transcripts UPF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF10">
        <property type="entry name" value="MIF4G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="3">
        <location>
            <begin position="65"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="3">
        <location>
            <begin position="468"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="3">
        <location>
            <begin position="671"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="323"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="388"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="890"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1039"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="892"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="935"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT61510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1192" mass="134931" checksum="0AD9B59470A01227" modified="2012-06-13" version="1">MDYMENESHEEKKQDEEEHHNKQANEEARLEEYKKLIDVKAALRQSNLNPERPDASYLRTLDSSIKRNTAVIKKLRTITNEQKDGLMEELKSVNLSKFVSEAVSYICEAKPRSADIQATVQICSLLHQRYKDFSPCLVQGLLKAFFPGKSGDCLALDKNSRAMKKRSTLKLLMELYFVGIVDDATTFINIVKDLTSVEHLKDRETTQTNLSLLASFARQGKHFLGLQKHGQEASDEFFVGLSVTDDVKSFFKKALSSYYDATTVVLQSEHASLRVMESENSKILNAKGELSDENTALYEKLRKSFDQLLHCVSSLAEALDMQPPVMPDDGHTTRVTTGTDLSPTGKESSTVESIWDDEDTKAFYESLPDLRVFVPGVLLGEAEPKLVDQHGKVHEQTSESTLEHGNEVQDNAETSVPEHQLEVKANIISKNREDKEKVDKGKSKEKDAGRKEDIESDKVRVTDGASLDNLLQRLPRCVSRDLIDQLTVEFCYLNSKANRKKLVRTLFNVPRTSLELLPYYSRLVATLSKCMKDLPSMLLPMIEEEFNFLINKKDQIKIETKIRNIRFIGELCKFKIAPSGLVFSCLKACLDDFSHHNIDVACNLLETCGRFLYLSQGTTIRMSNMLEILRRLKNVKNLDPHHSTLVENAYYLCKPPERSARVSKVRPPLHQYIRKLLFSDLDKTSVQHVLRQLRKLPWAECEQYLVKCFLKVHKGKYSQVHLIALLTAGLSHYHDGFPVAVIDEVLEEIRVGLELNDYAMQQRRLAHMRFLGELYNYEHIDSSVIFETLNLIIVFGCGTVEQDVLDPPEDCFRIRMIITLLQTCGHYFDRGSAKRKLDRFLLYFQRYVLNKGPLSLDVEFDVQDMFAELRPNMTRYSSTEMLDSVLAELEENEHGSSAEKGGCERDSDNRPQMKQSESAAFDANCKRLVNRPNKNGRDHEAAADSESHSGRGSVYRDGHEDENFLYEEKSDDMSEIGVDNDYGCMLVGSDEESFQVRHKVVQVDPKEQEDFDRELKALVQESLESRKSELRAKPTLNMTVPMNACDGSKDPKATGAESGQEITAEESGSAGSGSKVKVCVRVLVKKGHRQQTKQMLIPGDCSLVQSTKQQEAALHQEKQNIKQKILEYNEREEEESKGEPLQTGYWGQGASSTGSSIRSAGCGAWDGANRGGGNRQRCYIAGGIYHGYGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2024-03-27" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EAF6</accession>
    <name>A0A0Q3EAF6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g22854v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EAF6"/>
    <dbReference type="STRING" id="15368.A0A0Q3EAF6"/>
    <dbReference type="EnsemblPlants" id="KQJ84791">
        <property type="protein sequence ID" value="KQJ84791"/>
        <property type="gene ID" value="BRADI_5g22854v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ84791">
        <property type="protein sequence ID" value="KQJ84791"/>
        <property type="gene ID" value="BRADI_5g22854v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EAF6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ84791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="135" mass="14423" checksum="363F752F61C315E4" modified="2016-01-20" version="1">MASPKLAAGDGSYDFHLRSLSAASRDSAAAADPASDPKLLQSVRRMVARAFPVVRKLFLRCAAAPTLSTASSGMLLLERKGSEFSESQGGPRKFRRRSRSAHSVCASLPGQSLEPRDVCSLLSFCLCLKAPGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ECD8</accession>
    <name>A0A0Q3ECD8_BRADI</name>
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        <recommendedName>
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    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glutathione peroxidase family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQJ85383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ECD8"/>
    <dbReference type="STRING" id="15368.A0A0Q3ECD8"/>
    <dbReference type="EnsemblPlants" id="KQJ85383">
        <property type="protein sequence ID" value="KQJ85383"/>
        <property type="gene ID" value="BRADI_5g26725v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832728"/>
    <dbReference type="Gramene" id="KQJ85383">
        <property type="protein sequence ID" value="KQJ85383"/>
        <property type="gene ID" value="BRADI_5g26725v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832728"/>
    <dbReference type="OrthoDB" id="446890at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3ECD8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutathione peroxidase"/>
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        <property type="entry name" value="GPX_CS"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <position position="46"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ85383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="171" mass="19083" checksum="CF5CA66CF94137D5" modified="2016-01-20" version="1">MGAAESSSKPVGSVHDFIVKDAKGNDVELSRYKGEVLLIVNVASRCGLTNSNYTELGQVYEKYRDKGFKILAFPCNQFAGQEPSSNEQIVEFACNRFKAEFPIFGKVDVNGNNAAPLYKFLKSERGGLFGERIKWNFTKFLVDKEGHVVNRYAPTCSPFNIENDIKKLLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EGZ1</accession>
    <name>A0A0Q3EGZ1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC104584414</name>
        <name evidence="1" type="ORF">BRADI_4g09013v3</name>
    </gene>
    <organism evidence="1">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Stipodae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3EGZ1"/>
    <dbReference type="EnsemblPlants" id="KQJ87015">
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        <property type="gene ID" value="BRADI_4g09013v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584414"/>
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        <property type="protein sequence ID" value="KQJ87015"/>
        <property type="gene ID" value="BRADI_4g09013v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584414"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EGZ1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012535">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="EMBL" id="KQJ87015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="150" mass="16116" checksum="0DAD3173984C65F2" modified="2016-01-20" version="1">MVPLLFGRGMLGMGRMEVTARGGEGSSLQLEGSGCGDGELAGAQVRWRLSRESTLARHTGLRGAMADLSFLLARGPPNLLGFLRSTASASGYSQLLLFRHSQSIPDLQGFDIAITSGCFDVGPSCLAPVRRNACESVFFWVYCRLAHYCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GSQ1</accession>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim17/Tim22/Tim23 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_1g13010v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK13871"/>
        <property type="gene ID" value="BRADI_1g13010v3"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="PANTHER" id="PTHR10485:SF0">
        <property type="entry name" value="AT05822P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="111"/>
            <end position="133"/>
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    </feature>
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        <location>
            <begin position="148"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="156"/>
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        </location>
    </feature>
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        <source>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK13871.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="168" mass="17747" checksum="471D211F88A67D5D" modified="2018-03-28" version="2">MPHPDIKRCPDCIIDSAGCCFVAGAIGASAYHFPKGLYNSPNGQRLAAGARAVRINAPRVGGSLAAFGVCLEAFRCAMLSARKTDDFWSYVLPWFAAGICVPVPLGRGPRAVAISALTSLSFSVLAYGLRFCIGRVDVASLFPPPSVDSGPMGRQGRIEKTETTARRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GV74</accession>
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            <fullName evidence="1 2">Uncharacterized protein</fullName>
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        <name evidence="1" type="ORF">BRADI_1g15150v3</name>
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            <taxon>Poaceae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Rokhsar D."/>
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            <strain evidence="1">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
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        <property type="expression patterns" value="baseline"/>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="KQK14285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="216" mass="22744" checksum="C113AD7B995F38D8" modified="2016-01-20" version="1">MPLDQHYNLEEQCSSKQPKEAGMASTSGSISVGVSQVAFVLAAIASLSLIPSSLAIASSLPLGAALSARELLAALCGLVATKKALFVLCNVIFLFLAADCRCFSGICSLSPSANDAVDDGLTASESLPCAVPQPSEDVAVSSLRPPEAKSVPLETAVVVEESACSEAAQQELEKLEIDELNRKFEEFIQSRRIKWQQEEALPLQCQTANAKASSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GXB0</accession>
    <name>A0A0Q3GXB0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g24143v3</name>
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    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_1g24143v3"/>
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        </location>
    </feature>
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        </location>
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    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK15647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="125" mass="12392" checksum="3E7B10B79681E61C" modified="2016-01-20" version="1">MGVVSSTVPAALLLTSFLLISKLPRCRPLSFSYNFSDSATYDRADITTEGAATYSPTVAAGYSGKRNPAAAGSPAPGDSSSAGASSSSFTTGGARSTATSSSTTVGPSSCCPESSVVSMQQTAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H3M3</accession>
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    </protein>
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        <name evidence="7" type="ORF">BRADI_1g34581v3</name>
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    <organism evidence="7">
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">EF-1-beta and EF-1-beta' stimulate the exchange of GDP bound to EF-1-alpha to GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">EF-1 is composed of 4 subunits: alpha, beta (1B-alpha=beta'), delta (1B-beta), and gamma (1B-gamma).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17463.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H3M3"/>
    <dbReference type="STRING" id="15368.A0A0Q3H3M3"/>
    <dbReference type="EnsemblPlants" id="KQK17463">
        <property type="protein sequence ID" value="KQK17463"/>
        <property type="gene ID" value="BRADI_1g34581v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17463">
        <property type="protein sequence ID" value="KQK17463"/>
        <property type="gene ID" value="BRADI_1g34581v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H3M3"/>
    <dbReference type="OrthoDB" id="331763at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000001">
        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049720">
        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF21">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="6">
        <location>
            <begin position="6"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00058380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK17463.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="95" mass="10372" checksum="19E379A4737C83A6" modified="2018-03-28" version="2">MAAVGKSSVLLDVKPWDDETDMVKPEEAVRSVEKEGLTWGASKLVPVGFGVNKLQIMITVVDELVSVDDLIEDCLCAEPVNEYVQSCDIASFNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HQ32</accession>
    <name>A0A0Q3HQ32_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g17987v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HQ32"/>
    <dbReference type="STRING" id="15368.A0A0Q3HQ32"/>
    <dbReference type="EnsemblPlants" id="KQJ95594">
        <property type="protein sequence ID" value="KQJ95594"/>
        <property type="gene ID" value="BRADI_3g17987v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95594">
        <property type="protein sequence ID" value="KQJ95594"/>
        <property type="gene ID" value="BRADI_3g17987v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HQ32"/>
    <dbReference type="OrthoDB" id="2095648at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000037">
        <property type="entry name" value="F-box protein SKIP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF77">
        <property type="entry name" value="OS08G0195000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ95594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="261" mass="29325" checksum="3A955BF274A08DCC" modified="2016-01-20" version="1">MRKVIAPNGKIGKDAKEAVQAFVSEFIAFVTREASGKCRKEKQEAITGDHLLWAMATLGFQDYIQPLKLYLQKYRGVLPRKMVAEPNPLPVPEVRDWSELTVDALSAIFTKLGTIEILMGAGLVCHSWLEAAKLTELWRFEDMTGHNVVFSKAGNVMCKMAKVAIDRSDGRMESFWAEKFVSSELLDYIASRGNSLKSIQIISSGCFQDDKVARLAAKCPMLEEIECSHQKQPAYFYKQLGAVRPELKRLRIVCLGSTQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HWC9</accession>
    <name>A0A0Q3HWC9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100826247</name>
        <name evidence="8" type="ORF">BRADI_3g33106v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the expansin family. Expansin B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234989.1">
        <property type="nucleotide sequence ID" value="XM_010236687.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HWC9"/>
    <dbReference type="FunCoup" id="A0A0Q3HWC9">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ97762">
        <property type="protein sequence ID" value="KQJ97762"/>
        <property type="gene ID" value="BRADI_3g33106v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826247"/>
    <dbReference type="Gramene" id="KQJ97762">
        <property type="protein sequence ID" value="KQJ97762"/>
        <property type="gene ID" value="BRADI_3g33106v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826247"/>
    <dbReference type="OrthoDB" id="595323at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HWC9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22275">
        <property type="entry name" value="DPBB_EXPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005795">
        <property type="entry name" value="LolPI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF12">
        <property type="entry name" value="EXPANSIN-B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00829">
        <property type="entry name" value="LOLP1ALLERGN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129267" evidence="5">
        <location>
            <begin position="25"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="6">
        <location>
            <begin position="64"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="7">
        <location>
            <begin position="183"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ97762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="269" mass="29405" checksum="1985029790A0AD44" modified="2016-01-20" version="1">MAMAGAVALMSVLVATYGACAAAAQQEQQPAENYDTSAAYSSGWLPARATWYGAPTGAGPDDNGGACGFKNVNQYPFSSMTSCGNQPLFKDGKGCGSCYQIRCDKDQSCSGNIKTVMITDMNYYPVAQYHFDLSGTAFGAMAKPGLNDKLRHSGIIDIQFRRVPCNFPGLKINFHVVEGSNAVYLAVLIEYEDMDGDLTQVDMKEANSGQWVPMRESWGSIWRMDSNHRLQAPFSMRITSDSGKQLVANNVIPANWRANTDYRSFVQFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I094</accession>
    <name>A0A0Q3I094_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g06843v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I094"/>
    <dbReference type="FunCoup" id="A0A0Q3I094">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93811">
        <property type="protein sequence ID" value="KQJ93811"/>
        <property type="gene ID" value="BRADI_3g06843v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93811">
        <property type="protein sequence ID" value="KQJ93811"/>
        <property type="gene ID" value="BRADI_3g06843v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3I094"/>
    <dbReference type="OrthoDB" id="430364at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34996:SF2">
        <property type="entry name" value="OS02G0193732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34996">
        <property type="entry name" value="OS06G0327400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ93811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="108" mass="12524" checksum="7166DEE70A9F849B" modified="2016-01-20" version="1">MNRKESRRGRRLRHVRLGSLLRLRVRLFRLVGMVARCLEELNWCPGRRSMPLTTRSAGRCRHRPAALPAAHRENSASFHADAIADCLEFIKRSYMKPVQDDHRASTDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I0B4</accession>
    <name>A0A0Q3I0B4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100839004</name>
        <name evidence="14" type="ORF">BRADI_3g07031v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. Binds to the hexamer motif 5'-ACGTCA-3' of histone gene promoters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Binds DNA as a dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571241.1">
        <property type="nucleotide sequence ID" value="XM_003571193.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I0B4"/>
    <dbReference type="STRING" id="15368.A0A0Q3I0B4"/>
    <dbReference type="EnsemblPlants" id="KQJ93843">
        <property type="protein sequence ID" value="KQJ93843"/>
        <property type="gene ID" value="BRADI_3g07031v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839004"/>
    <dbReference type="Gramene" id="KQJ93843">
        <property type="protein sequence ID" value="KQJ93843"/>
        <property type="gene ID" value="BRADI_3g07031v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839004"/>
    <dbReference type="OrthoDB" id="643223at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3I0B4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000019">
        <property type="entry name" value="BZIP family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025422">
        <property type="entry name" value="TGA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693:SF22">
        <property type="entry name" value="DOG1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693">
        <property type="entry name" value="TRANSCRIPTION FACTOR TGA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14144">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51806">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="12">
        <location>
            <begin position="153"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="DOG1" evidence="13">
        <location>
            <begin position="218"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="92"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ93843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="464" mass="50382" checksum="FCC7D3CA10C3E1D3" modified="2016-01-20" version="1">MGDRPWQQPHEQLAASCSAHTGMIQASSAAAATSSIHGNIIRKDPGGGYDDMAELDQALFLYLNSQDQTSVQEQPQTLNIFPSRPMHVVVEPSPRAAANPPPNVAAGSSKQQPSRPAAPAPGKNGKAAVKREGSGGGGGTPSTSEQEGPRTPNAKTLRRLAQNREAARKSRLRKKAYIQNLETSRIRLSQMEQEMQRCSAQGAILGGGAGIGGLSPEAAWFDGEHARWVEEHERMMRHLRAAVELDDNNLQHQQGHQDDGGQLRQLVDAAAAHHVVLAELKSAVARADVFHLVSGTWLPAAERCFLWIGGSRPSDLVKVVLRHVEPLTEQQVASVCDVQRWVREREEALDQELQAARLSLSDVVCSDALLSPYPDMAAYMAHMSLAIANLSSLEAFVRQADTLRLQMLHRLPQILTARQAARCFLAIADYSQRLRALSSLWLARPRQGQGGQPTPPGAGGRIHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I735</accession>
    <name>A0A0Q3I735_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g03483v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81856.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblPlants" id="KQJ81856">
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        <property type="gene ID" value="BRADI_5g03483v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_5g03483v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ81856.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="119" mass="12683" checksum="75D06B0760CD5E50" modified="2018-03-28" version="2">MRKEFLYRRQGAAPVARPSRGLLRAQRERGRGRNFANIMRAPFGVSGLLCWAHGPVLPSLSCLLSGDGGAAAVDLCPLLPPLAVLLAEQRRWGAGPVDLSFPPLPAPATPGWKAVMDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IV11</accession>
    <name>A0A0Q3IV11_BRADI</name>
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            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">BRADI_4g27940v3</name>
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    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3IV11"/>
    <dbReference type="EnsemblPlants" id="KQJ89824">
        <property type="protein sequence ID" value="KQJ89824"/>
        <property type="gene ID" value="BRADI_4g27940v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89824">
        <property type="protein sequence ID" value="KQJ89824"/>
        <property type="gene ID" value="BRADI_4g27940v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IV11"/>
    <dbReference type="OrthoDB" id="649300at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IV11">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45224">
        <property type="entry name" value="OS01G0527900 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45224:SF10">
        <property type="entry name" value="OS09G0317700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="124"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
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            <begin position="91"/>
            <end position="122"/>
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            <end position="282"/>
        </location>
    </feature>
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            <end position="442"/>
        </location>
    </feature>
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            <begin position="269"/>
            <end position="280"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="415"/>
            <end position="425"/>
        </location>
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            <end position="437"/>
        </location>
    </feature>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ89824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="536" mass="61753" checksum="6DC5640DCD7FFEAC" modified="2016-01-20" version="1">MSPRPPGGSLNRKHCPNEPTSTPSHVNFCHPQFPQHMQFSQPPYAMNIPFQQFPQQPLYPPNVHYVVVQPQYAPYSLPPPLPPPQPALVMRSTSVSDSGTPHVETCPEEHGIDNVDNDDSAEPERTARRLAWTEKEDIRLIKKYDMYWENVAAAYNSTTPRDRRREAKHLKCHWHKITKKIACFDDCWCQVKAKYPSSLSDNMQLMDKTWVMFNEEARAIYLEEEKHCFAFDHCWKAVWDQPKWKAYISSLSSKTTKLSESGDYTSSTEDTEDYPEEMDEQGCATAKEIHEGKGKMPSSSEVEKDIQCSVDLHNTLQVNREEMTGVQLMHSDQKLELSKLEQTERADNSSFISKNQQELLMADAAWINEISARERAGYKELDYGRATVRENIPEKETGPQVCNKQEHGRAVRGNAPQSETPTQSCKAAKLKRKRKGKSVPCSSEVQEDIKRAVDLQIMLTKDREKMSEVQLRLSKEKLEFAKLKQQEAKDKKETTLYDKYSELLMSDTSRFNELQKAEYEKAVRRMGEMLFGRDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IVG7</accession>
    <name>A0A0Q3IVG7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC104585088</name>
        <name evidence="5" type="ORF">BRADI_4g28883v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQJ90000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239347.1">
        <property type="nucleotide sequence ID" value="XM_010241045.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IVG7"/>
    <dbReference type="SMR" id="A0A0Q3IVG7"/>
    <dbReference type="STRING" id="15368.A0A0Q3IVG7"/>
    <dbReference type="EnsemblPlants" id="KQJ90000">
        <property type="protein sequence ID" value="KQJ90000"/>
        <property type="gene ID" value="BRADI_4g28883v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104585088"/>
    <dbReference type="Gramene" id="KQJ90000">
        <property type="protein sequence ID" value="KQJ90000"/>
        <property type="gene ID" value="BRADI_4g28883v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104585088"/>
    <dbReference type="OrthoDB" id="1893065at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
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    </dbReference>
    <dbReference type="Pfam" id="PF03195">
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    </dbReference>
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        <location>
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            <end position="168"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="147"/>
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        </location>
    </feature>
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        <location>
            <begin position="20"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ90000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="382" mass="41558" checksum="892ABC6AD71EDE3B" modified="2016-01-20" version="1">MTSNSNTKQPPPPTRHLHITTPNPTSSSATTSSHSSRSPRVSTTAGGASSPSSGSGHHQNPNQQQNQACAACKYQRRKCNPDCPLARYFPADQQLRFLNAHRLFGVSNIQRTLRETPPELRTDAMRALIFQAEVRAYDPVGGCCRMAIDLQRQLDLAQAELSALLQQLELCRRQQHHAVDDDDQMLLVAGAGNGQDVVDDVAADALYANGAGGQENVVLGQANLGEHYLLEEQGQPEPEPHQLYDYFYYDGTAGDETSSHAWTNDVHAHQQQYGNNGGSPVELVEQLEQHCQIETQFVDVFDVKAERAAASSTAIESHINADNGNASQFEDLHENEEKAMVSTAVVKSELDHQVAAAAQMAESSGTGSHCQLELGFSSSNAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J900</accession>
    <name>A0A0Q3J900_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7597 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="3">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_2g44843v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08884">
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        <property type="gene ID" value="BRADI_2g44843v3"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="entry name" value="DUF7597"/>
    </dbReference>
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        <property type="entry name" value="OS02G0303350 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="402"/>
        </location>
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        <location>
            <begin position="249"/>
            <end position="277"/>
        </location>
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        <location>
            <begin position="491"/>
            <end position="528"/>
        </location>
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        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
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        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24530"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK08884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="539" mass="60829" checksum="C7722B1B215B4707" modified="2018-03-28" version="2">MAALDLLAMERRPSPCLPPLLSGRCKFRLTEVSVGNLLNVCLGEVPEDFRVQHLCDRTFRFSVTNKIIGFHISSLKSFTRSFFVVYFHLWGFGGPDYVKEFYSWRQEEQLLWESPKKSLSPVKAHKSYAQAVANGGSILTSANAIPIQRPSALHRLSFNATSSSLNPWSLTDEVDLADVGYSEEDIQQCKKDHLLQVDRLQKKPIDIGTVFKRLQFLAASPAPAPEKNRFESENGPRHYGGLNSGVIKPSAHSQGTVSQASFPRQIDGPRDDARPTCTRCLRTGHTSGRRFDIVELAGRPQHGRYHIRGQIERANEDVAIVTLNPPPHPDDPFAVTRGIISHFVGEHLHLRVEHLHRCSLGHAYIRMNSTADKDWLVQHSPMVHNGISFSVTEHNRGTNWRGFTYNQEVWLMLLGYHLDLWSAEHLANVVSEWGKMILWDRSLSNMNRIILKVKVANVTMIPYSILLSHGADFQGPVFILSSSLLGVIPPDEQDPPENGQYKKSKKLLNDSTTTKDKKTKKDKKPPRNGWFQGYLSCFA</sequence>
</entry>
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        </submittedName>
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JJB6"/>
    <dbReference type="EnsemblPlants" id="KQJ98376">
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        <property type="gene ID" value="BRADI_3g36542v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ98376">
        <property type="protein sequence ID" value="KQJ98376"/>
        <property type="gene ID" value="BRADI_3g36542v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JJB6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JJB6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ98376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="178" mass="20549" checksum="CF4EDE622E5F01ED" modified="2016-01-20" version="1">MPFTCPWQKRRASNHVIWWRTQASQPIQIRQVPSSTSSLRGPLCGFQAMPAFKQKSSDASSESKRRQPIEGAIAPSLAHRLNQLSWRHREGLLPPVLEVFVREQIPSFRQRRAMESRRRQRQPARKHFIRRVAIRTSGVDTCVVYVSNRDRVVISSSIWVHEPSSGCNFARRSFDLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JN31</accession>
    <name>A0A0Q3JN31_BRADI</name>
    <protein>
        <recommendedName>
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    <gene>
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        <name evidence="3" type="ORF">BRADI_3g45061v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317646.1">
        <property type="nucleotide sequence ID" value="XM_024461878.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_024461880.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317649.1">
        <property type="nucleotide sequence ID" value="XM_024461881.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317650.1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQJ99752">
        <property type="protein sequence ID" value="KQJ99752"/>
        <property type="gene ID" value="BRADI_3g45061v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g45061v3"/>
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    </dbReference>
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        <property type="entry name" value="Zn-ribbon_GIR1"/>
    </dbReference>
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        <property type="entry name" value="OS02G0536500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33177">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="197"/>
        </location>
    </feature>
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        <location>
            <begin position="83"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
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        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ99752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="227" mass="24834" checksum="AE1CF4637417C373" modified="2016-01-20" version="1">MEELLPHCVCILQHLRTPIDLPPISPSISPYKYLQISSFLPPPLAQRSPKEPSSQHSRFCICKSSHGKARLLCTRYLTDSDSDLGGRKERMSRGNGGGGGRGLDLKLHLSLPARGDSSSTMRRMLAAPADDESSPSSCLSSESEPHGGVGALQWSDSPEATSMVLAACPRCFIYVMLAEADPRCPRCRSPVILDFLHAGNGNANEDHGRDNNNNNQNRRSRRNGRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JT41</accession>
    <name>A0A0Q3JT41_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Knottins-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g56893v3</name>
    </gene>
    <organism evidence="4">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK20811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="79" mass="8603" checksum="CB7AFF8970E5DFD3" modified="2016-01-20" version="1">MDLSVKFCVIVLLVLVTTGVFLNMGLVQVALARDCEAPSHKFHGACVRDSNCASVCETEGFSGDKREGPLMRCYCTKQC</sequence>
</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">Bd21</strain>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
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        <property type="gene ID" value="BRADI_4g29205v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2822301at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="228" mass="24696" checksum="BD02A3A9DCC0945F" modified="2016-01-20" version="1">MARTGRCSGSHPRPPNRRRPHPFLGSNDSHRQPPPPEPLDPVVLSTKSTDPAIPGPDLAAPALTVIATTTEREEERVTSPALPSQPAGTFPLVKCPCCHSRTVLHLVSGSSQNLVRVFYKCPNHRRGPKGCNFFHWEDGEDNYVECLASAGVNLGNVVVYGGDSGGLTEAKEEEEVGGQPAIESKNSASDVLLKELVQKMDEIIGLCRMMVVVLVVFVAMMMYDVVVK</sequence>
</entry>
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    <accession>A0A0Q3NG22</accession>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
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                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
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        <property type="protein sequence ID" value="KQK16334"/>
        <property type="gene ID" value="BRADI_1g28273v3"/>
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    <dbReference type="OrthoDB" id="675880at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </feature>
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        <location>
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            <end position="146"/>
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    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK16334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="161" mass="16772" checksum="DC101B926C395422" modified="2016-01-20" version="1">MAAAVLPPLLPTPPSSAMLPILPTPPRSKMLPLLPTPCGLAMLPKPGRADSVERWDANKNSASHSSASSSSSGGAGIPGRAGSFERWDSKKLGKSSTPMDQGRRSETETSNKRPPSRASSAEQTAASKEITEPAAAFAGPNFFATPEPSMLPMPTSLLRAH</sequence>
</entry>
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    </protein>
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        <name evidence="2" type="ORF">BRADI_2g60161v3</name>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="2 3" key="1">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RE02"/>
    <dbReference type="EnsemblPlants" id="KQK11426">
        <property type="protein sequence ID" value="KQK11426"/>
        <property type="gene ID" value="BRADI_2g60161v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11426">
        <property type="protein sequence ID" value="KQK11426"/>
        <property type="gene ID" value="BRADI_2g60161v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3RE02"/>
    <dbReference type="OrthoDB" id="581771at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RE02">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111">
        <property type="entry name" value="BNAA05G37150D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111:SF136">
        <property type="entry name" value="F-BOX ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box associated beta-propeller type 3" evidence="1">
        <location>
            <begin position="97"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK11426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="274" mass="30160" checksum="928C551753C71A6A" modified="2016-01-20" version="1">MQAAALLAVAAPYARYGGVGIVVPPHRLNRSDGGGALVARTVELEVKDGSMTHRGKGRLAATATGVEAAARDGEAATKSVEAALDAKIDERRGDQHKFACFVFTLGSGEQPRQIGSVYSWVEPEEMHESVLVCGSLHWYREELGSESNMILVFDTTGEVFWQMCAPVVSSSTRLFEMDGTLGMSTVNDEAKIVDIWVMKDYESEVWALKCRVELPVAEIRLQFGTYGNFWTAMVDFWNLIVVSVDGELLVLLAYDDWLLLVDMDGKLVDSSRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RJZ8</accession>
    <name>A0A0Q3RJZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ACT domain-containing protein ACR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Protein ACT DOMAIN REPEATS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100826395</name>
        <name evidence="5" type="ORF">BRADI_1g09860v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds amino acids.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560261.1">
        <property type="nucleotide sequence ID" value="XM_003560213.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RJZ8"/>
    <dbReference type="STRING" id="15368.A0A0Q3RJZ8"/>
    <dbReference type="EnsemblPlants" id="KQK13394">
        <property type="protein sequence ID" value="KQK13394"/>
        <property type="gene ID" value="BRADI_1g09860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826395"/>
    <dbReference type="Gramene" id="KQK13394">
        <property type="protein sequence ID" value="KQK13394"/>
        <property type="gene ID" value="BRADI_1g09860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826395"/>
    <dbReference type="OrthoDB" id="2019938at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RJZ8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04897">
        <property type="entry name" value="ACT_ACR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040217">
        <property type="entry name" value="ACR1-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096:SF7">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="137"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="4">
        <location>
            <begin position="380"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK13394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="506" mass="55349" checksum="E3A21418908E4018" modified="2016-01-20" version="1">MKYVSGPYFEPDFDPVLDRFGTPGVVVDNETREDCTLVKVDSVNRDGVLLEMVQLLTDLDLVISKSYISSDGGWLMDVFHVTDQIGRKLTDPSLPGFIQQALVPFQRRPGHGPSPKFTTCLGNVVGPGGPDVSDCASLEFTVPDRPGLLSSITQVLVDQGCHVASGQSWTHSGRAAGVLYVTMTAAAEAQPPHQSRWAHIERLVSAVVDARESVSGARRWVCMSAPAPGRVHTERRLHQLMHDDRDYESGPAPTPVDEEHFSMGDVRAATMMLMAARRSGARRGAADTRVTIDNWEERGYAVVKMTSRDRPKLLFDTVCGLTDMQYVVFHATVGSQGPLAIQEYYIRHKDGRTVDSNAERQKVSRCLVAAVERRASHGVRVEVRAADRSGLLSDFTRILREHGLSLLRVELKRQKDEAVGTFYLVTDSGGEVRPEVVRAVRARVGEMGISLEVAKEAPGWPPVRKTSVPVQPAAAGQGQEGSGSSLGSLLWSHLGKLSNNFGYIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CFT2</accession>
    <name>A0A2K2CFT2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g07630v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CFT2"/>
    <dbReference type="EnsemblPlants" id="PNT60885">
        <property type="protein sequence ID" value="PNT60885"/>
        <property type="gene ID" value="BRADI_5g07630v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60885">
        <property type="protein sequence ID" value="PNT60885"/>
        <property type="gene ID" value="BRADI_5g07630v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CFT2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT60885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="134" mass="15711" checksum="D09ED96D8530D080" modified="2018-03-28" version="1">MTSTSRHMILLKNHNTHSKRNHEIQKQDHSTRFKKIQKHTHPVQSAARHPDPADRLVHEHGQRRLRAKWWGRRRRREEMRASWGWERERDAREREEGGVEAVGSGAVHRGVLARGGWWGGSRRGGTLAELKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGY1</accession>
    <name>A0A2K2CGY1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_5g13265v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CGY1"/>
    <dbReference type="STRING" id="15368.A0A2K2CGY1"/>
    <dbReference type="EnsemblPlants" id="PNT61292">
        <property type="protein sequence ID" value="PNT61292"/>
        <property type="gene ID" value="BRADI_5g13265v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61292">
        <property type="protein sequence ID" value="PNT61292"/>
        <property type="gene ID" value="BRADI_5g13265v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CGY1"/>
    <dbReference type="OrthoDB" id="65569at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CGY1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033907">
        <property type="term" value="F:beta-D-fucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000020">
        <property type="entry name" value="Beta-glucosidase 12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF154">
        <property type="entry name" value="BETA-GLUCOSIDASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033311379" evidence="8">
        <location>
            <begin position="32"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT61292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1061" mass="120171" checksum="F05052D235ABDED6" modified="2018-03-28" version="1">MGGGGRQHCWSNKHMDGRLVLPFLLLAVASAAYDAAGRTPIGRTSFPKGFVFGTSSSSYQYEGGAMEGGKGPSIWDNFTHQHPGIRNHIASLSGPDGTCFSEDDGKNVLLWEPYKGRLGQSDDYDLKKLYIKITHRTCSLNFLQEDVRLMKDMGMDAYRFSISWTRILPNGTLRGGVNTEGIKYYNSLINELLCKGVQPFVTLFHWDSPQALEDKYGGFLNPNIINDYKDYAEVCFREFGDRVKHWITFNEPWAFSVGAYAMGVLAPGRCSPWELGKCDAGDSGREPYIAAHHQILAHASAVRIYKEKYQALQKGKIGVSLVSHWFVPFSCSNSDNDAARRAIDFMLGWFMDPLTSGNYPMSMRGLVGNRLPQFTKEQSRLVKGAFDFIGINYYSANYADDLPPSNGLNISYNTDARVNLTGVRNGVPIGPQYASPGLYVYPQGLRDLLLYVKGHYGNPDIYITENGFNEVNNKSLPLQESLMDDARVEYHHKHLDILQSAIRDGANVKGYFAWSLLDNFEWVYGYTMRFGLHFVDYDDERKRYPKHSARWFKKYLQVKRPLVAMLARLVLPFLLVAAASGAGHQELISRRSFPEGFVFGTASSSYQYEGGAMEGGRGPSIWDNFTHQHPDKITDRSNGDVAADSYHLYKEDVRLMKDMGMDAYRFSISWTRILPYGTLRGGVNREGIKYYNNLINELLSKAICHPFHWDSPQALEDKYGGFLNPNIINDYKDYTEVCFREFGDRVKHWITFNEPWSFCVTGYARGVFPPGRCSPWEKGNCSSGDSGREPYTVCHHQLLAHAATARLYKAKYEALQKGKIGISLVSNWFLPLSRSKSNGDAAIRSVEFMLGWFMDPLIRGDYPLSMRRLVGNRLPRFTKEQSELVKGAFDFIGINYYTTSYADNLPPSNGLKNSYNTDARANLTGVRNGVAIGPQAASPWLYVYPPGFRELLLYVKKNYGNPIVYITENGVYEANNKSLPLKEALKDDARIEYHHKHLLALLSAIRDGANVKGYFAWSLLDNFEWASGYTVRFGLNFVDYCDGQKRYPKNSARWFRNFLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHD9</accession>
    <name>A0A2K2CHD9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">methylated diphthine methylhydrolase</fullName>
            <ecNumber evidence="6">3.1.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100836424</name>
        <name evidence="9" type="ORF">BRADI_5g15180v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphthine methyl ester-[translation elongation factor 2] + H2O = diphthine-[translation elongation factor 2] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42656"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="3.1.1.97"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH7 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.97" evidence="6"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHD9"/>
    <dbReference type="STRING" id="15368.A0A2K2CHD9"/>
    <dbReference type="EnsemblPlants" id="PNT61438">
        <property type="protein sequence ID" value="PNT61438"/>
        <property type="gene ID" value="BRADI_5g15180v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61439">
        <property type="protein sequence ID" value="PNT61439"/>
        <property type="gene ID" value="BRADI_5g15180v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61440">
        <property type="protein sequence ID" value="PNT61440"/>
        <property type="gene ID" value="BRADI_5g15180v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61438">
        <property type="protein sequence ID" value="PNT61438"/>
        <property type="gene ID" value="BRADI_5g15180v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61439">
        <property type="protein sequence ID" value="PNT61439"/>
        <property type="gene ID" value="BRADI_5g15180v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61440">
        <property type="protein sequence ID" value="PNT61440"/>
        <property type="gene ID" value="BRADI_5g15180v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1930760at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CHD9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061685">
        <property type="term" value="F:diphthine methylesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052415">
        <property type="entry name" value="Diphthine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042:SF1">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="264"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT61439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="407" mass="45200" checksum="94A5EF96B90FDC95" modified="2018-03-28" version="1">MATEPRRCRRPPAWLDAGETATTEIAAAKVPMAKRFRVLESLPPPPRAKRGLEMDLGSFHLGGNADVVEFCPHRPFRHILAAATYTLQEQVTEEQQQDRAGTVSLFSVDAGAEDESQRLRLLHTVDTAGVFDMKWSPTAPLLAQADAHGRLVLRRLEQEDGSDKGVVLTDVCSGDVSSSMCLYVDWNQNADSLSVGLSDGSLSVISMREDCLEVSEHWTAHQYEVWTCYFDRARPHLLYSGSDDCSFSCWDLRESPSNAVFRDKKSHSMGVCCIAQNPLDGNMLLTGSYDEFLRVWDMRSMVKPVNEKSLNLGGGVWRMKYHPSIADVVLSACMHNGFAIVKVGAGDATVMETYCKHESLAYGADWQTSEAPEHSTASSVIATCSFYDRLLRVWQPEELVKHPGFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CIF0</accession>
    <name>A0A2K2CIF0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nuclear pore complex protein NUP88</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_5g21070v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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        <subcellularLocation>
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    </dbReference>
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</entry>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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                <consortium name="EnsemblPlants"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <accession>A0A2K2CKU2</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
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                <person name="Shu S."/>
                <person name="Lindquist E."/>
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                <consortium name="EnsemblPlants"/>
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        </citation>
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            <begin position="99"/>
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        </location>
    </feature>
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            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT62640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="152" mass="16494" checksum="1CA8D10A854967C6" modified="2018-03-28" version="1">MSKHSSSKHSSAIIALDSGQLSRHLLRPLKPCSWPPSWPCPFPSSSAPHLPSSTIFFLTGQWCVSLLNGDAGGCEGKVHGVRRLFTRVGALESSGEARHTPACSARDAGGCGDEVHRPRGRGRDLRDRGQRSHCWTIVRVGNNHTPPRPCLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMK3</accession>
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CMK3"/>
    <dbReference type="FunCoup" id="A0A2K2CMK3">
        <property type="interactions" value="209"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63249">
        <property type="protein sequence ID" value="PNT63249"/>
        <property type="gene ID" value="BRADI_4g13441v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63249">
        <property type="protein sequence ID" value="PNT63249"/>
        <property type="gene ID" value="BRADI_4g13441v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47069:SF11">
        <property type="entry name" value="OS04G0275550 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="3"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="239" mass="27442" checksum="DB439E2F5FED1E36" modified="2018-03-28" version="1" fragment="single">PTKTLSVTGYKNLSEEFHKQTGRLYTRKQFKNHWDELKSIWTAWNTITASEEQWADLIKVHKDIRRFRKGPPDNLEWLEKMFQKASVTGNSSVMPGAEEDDIAEHQDVDGIVDLEADDEGTPQPSPLPMPKMKRGGASAIPCSPKRPKKDPYGKDFKRFVDHVISEEKSEASSNVLVANDIDAIMEEVVKCGAPETSDEYYIATKLFGKLENRCFFYAMKTSEGRLQWLKRQYADRKRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMW8</accession>
    <name>A0A2K2CMW8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g14953v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CMW8"/>
    <dbReference type="STRING" id="15368.A0A2K2CMW8"/>
    <dbReference type="EnsemblPlants" id="PNT63377">
        <property type="protein sequence ID" value="PNT63377"/>
        <property type="gene ID" value="BRADI_4g14953v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63377">
        <property type="protein sequence ID" value="PNT63377"/>
        <property type="gene ID" value="BRADI_4g14953v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CMW8"/>
    <dbReference type="OrthoDB" id="1748457at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF16">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20870" checksum="78F3159982338B44" modified="2018-03-28" version="1">MLSRSGFGWDWDNNKISVDSDDVWNTYVQANEDATSYRHKTIRFWDMISLVYSKNRANGAGARTAAESVVEMAAENGDNSKDTDATSSTQVDGDRKKKRYRSDETIASMLGEKLDNFTDAFQADVCEPPPKPASPEEILAALDAIPGLGEDDVLAAYDILVSNDRKFKSLQALPDGMKKKWILKQVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CN92</accession>
    <name>A0A2K2CN92_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836795</name>
        <name evidence="3" type="ORF">BRADI_4g16910v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311141.1">
        <property type="nucleotide sequence ID" value="XM_024455373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CN92"/>
    <dbReference type="STRING" id="15368.A0A2K2CN92"/>
    <dbReference type="EnsemblPlants" id="PNT63511">
        <property type="protein sequence ID" value="PNT63511"/>
        <property type="gene ID" value="BRADI_4g16910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836795"/>
    <dbReference type="Gramene" id="PNT63511">
        <property type="protein sequence ID" value="PNT63511"/>
        <property type="gene ID" value="BRADI_4g16910v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836795"/>
    <dbReference type="OrthoDB" id="599271at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170:SF18">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170">
        <property type="entry name" value="BNAC04G53230D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="452" mass="52357" checksum="85187ABF29D3AD8A" modified="2018-03-28" version="1">MLQDAWVVDMERKLDESEPSAKTEKWPKHSIFRVPPRFKMVHGRGEASIYMPQTVALGPFHHDNDGLKPMEEHKMRAVRHLLQRAQRTIGELVKSMEEVAQDLEDAYMDLGDEWRGENRVKFLEMMIADGCFLLEVMREEDSRKDYVNSDPIFSKKGVRHVQSFVQRDMLMLENQLPLKVLQRIVAVETGKPPSDGDVVINNILLKFLCTENWDPRVASNGLGLHPLDLYRRSLLKWPGPVHRGVAGILGRKRPPPDVPRSAQKLADAGIRFRTSRRKFQLDDIEFKTTRTVMMPRLYLDDTTHYKYHNMMAFEAMHVRTSNDVTAFVLFMKDIIDSAEDVAHLRRKRIIVHDLADDDAAVVKMFNTLTKDVPKYGDSRLCGVREEMEDYYKKSICKSLWFVPWSFLKNKHFRSPWTFIALVAAICLVATDIMQTFYAVMSYEVAKTSLQAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CPW3</accession>
    <name>A0A2K2CPW3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_4g24017v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CPW3"/>
    <dbReference type="STRING" id="15368.A0A2K2CPW3"/>
    <dbReference type="EnsemblPlants" id="PNT64064">
        <property type="protein sequence ID" value="PNT64064"/>
        <property type="gene ID" value="BRADI_4g24017v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64064">
        <property type="protein sequence ID" value="PNT64064"/>
        <property type="gene ID" value="BRADI_4g24017v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CPW3"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF492">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE PTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="7">
        <location>
            <begin position="429"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="8">
        <location>
            <begin position="609"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT64064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="711" mass="80405" checksum="864321FB62FEF2B3" modified="2018-03-28" version="1">MTMQPQEKAPLHMQCSDKFLVQSTRVSEACTTEDITSETFNKEMGKIVDEVKVSVAYVAPSQQLSTVWEGDSDTKDATLCNRNEDEDKEDSTLCEHDDDKEESEIVQTLDATNFDALIATHPFIVIEFCVPWFKDFEISAREHTFPLCNLSQYEKAAQVLSKNNPPIVLAKLDASDKNNRSILTKLGVKVIPTVKIVTNEGMNMQTYDGPRDADGIVEYSKRQIRPASMEIKSAKDVAELFNDDKLHIVGIFLEFTGSEFANFMEVAEKLRCDYEFHHTLNTTHLPGGGTVASSPLVRIFKPFDELVSDKFLFSPTDNKVVYRPSRGTTVPHPPSLPTTPKVVADENPDNLLPTLEVLQGSSTSSAWFDDLDANYSGSSSPLQAWYENQVHCLDPEMDPPPQMITPDQVKRCKKALKVLDKKLRQRATILQEFRSLPAIRTALLTQKFSVARSPANREKNCYTDVLPFDETRIRLQSSTGNHTAKNDYINASPTKLDNRNQTKFISTQGPLVNTFEDFWQMVFENCCPVIVMITKFDNVKCDEYLPLSEGEGDYGRFCIKIMKIRMDGQLVLRSLKVQCNESDRFTTVLHIQYSEWPDHGVPNDSSVVRKIMKRLHHIKSRRGFPIVVHGSAGLGRTGAYITIHNTIERILLGEQDALDVVETVRKFRSQRAGVVQTEEQLMFCYKAIVDELKELVQNSPVPSSSFSWKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQD4</accession>
    <name>A0A2K2CQD4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhamnogalacturonan endolyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g26420v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQD4"/>
    <dbReference type="STRING" id="15368.A0A2K2CQD4"/>
    <dbReference type="EnsemblPlants" id="PNT64234">
        <property type="protein sequence ID" value="PNT64234"/>
        <property type="gene ID" value="BRADI_4g26420v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64234">
        <property type="protein sequence ID" value="PNT64234"/>
        <property type="gene ID" value="BRADI_4g26420v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQD4"/>
    <dbReference type="OrthoDB" id="2130367at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CQD4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10317">
        <property type="entry name" value="RGL4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10316">
        <property type="entry name" value="RGL4_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10320">
        <property type="entry name" value="RGL4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051850">
        <property type="entry name" value="Polysacch_Lyase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029413">
        <property type="entry name" value="RG-lyase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029411">
        <property type="entry name" value="RG-lyase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010325">
        <property type="entry name" value="Rhamnogal_lyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32018:SF2">
        <property type="entry name" value="OS11G0134100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32018">
        <property type="entry name" value="RHAMNOGALACTURONATE LYASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14683">
        <property type="entry name" value="CBM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14686">
        <property type="entry name" value="fn3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06045">
        <property type="entry name" value="Rhamnogal_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="3">
        <location>
            <begin position="344"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="2">
        <location>
            <begin position="432"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT64234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="666" mass="72468" checksum="4EDBC5737F109EBD" modified="2018-03-28" version="1">MPARLTCGADLWGWGFIFFRKSTGEIMLDSTEFRVVTSTPDKVELSFRSAYDPTRRDSVRLAVDKRLVLLRGVSGFYPYAVFSHSAALPPLNITEARLAFKLGTSRFRYMAVSDKLQRAMPSAADRDPPRGRPLAYKEAVLLVNPAEPEFKGEVDDKYMYSLDNKDNKVHGWISEGTTSSSPAPASPAAVGFWVVTPSNEFKSGGPMKRELTSHVGPTALTMFMGTHYVGDAIVANIGDGENWTKVLGPVFVYLNSVPEKKQKQQKKSFWGGGRGAPVVMDPKAALWEDAKARAAAEAAKWPYNFPESPEFHKAGERATVAGRLLVRDRYLVAAGAGEDGMFPARNAYVGLAAPGQAGSWATESKGYQFWTRSAASGGFAITGVRAGLYSLYAWVPGVHGEFVSSYPVNVTGAGEVLKLGDLVFAPPRAGPTVWEMGIPDRSAAEFFVPDPNQKLVNRLFLGPAMAAEKFRQYGLWDRYAELYPNGADPVFTVGASHYSKDWFFAHVTRKLPVVVSGSGGQQQQQYAPTTRQIKFALAAVASDGNYTLRIALAAAQMARLRIEVNPPPLESGKTRRPAAVFTTPEFGEGNAIARHGDHGTWWSFEFGIQGRMLVEGENVISITQVRAFGAFLGVMYDYIRLEAPPASRRDPTAAAAAMNGTSSIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D1C6</accession>
    <name>A0A2K2D1C6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g35852v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D1C6"/>
    <dbReference type="EnsemblPlants" id="PNT68097">
        <property type="protein sequence ID" value="PNT68097"/>
        <property type="gene ID" value="BRADI_3g35852v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68097">
        <property type="protein sequence ID" value="PNT68097"/>
        <property type="gene ID" value="BRADI_3g35852v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D1C6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT68097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="77" mass="8931" checksum="ABE3A3DC48B7909F" modified="2018-03-28" version="1">MARVPPGRPYSPTNFHGSIRFTYCGSSFLRWNGASEHICATVKLMMPCLDFRREFVQKTLFLRLRASYITESFFVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D1M2</accession>
    <name>A0A2K2D1M2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g36830v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="635" mass="73467" checksum="0691FA25374D8126" modified="2018-03-28" version="1">MCFPMRALQSYRTTIMLSDDTTKRRTTKSKSFKDVENFEVLVLEKNCGCKFKSLRYLLIAIVSATFVTLLTPTLYEHQLQSSSLYVDVDWIWDKTSTDPRYVSSVNVQWMDVYKAIEDLNGGDQKLKVGLLNFNSTEYGSWSQLLPESHVSIIRLEHAKDSITWPVLYPEWIDEEEETEIPSCPSLPEPNVRKGVWFDVIAVKLPCTRVGGWSRDVARLHLQLSAAKLAVISSRGNRNVHVLFVTDCFPIPNLFPCKNLVKHEGNAWLYRPDLKALKEKLKLPVGSCELAVPLKAKARLYSVDRRREAYATILHSASEYVCGAITAAQSIRQAGSTRDLVILVDDSISDHHRKGLEAAGWKVRIIQRIRNPKAERDAYNEWNYSKFRLWQLTDYDKIIFIDADLLILRNVDFLFAMPEITATGNNATLFNSGVMVIEPSNCTFQLLMEHINEITSYNGGDQGYLNEIFTWWHRIPRHMNFLKHFWEGDSEAAKAKKTQLFGADPPNLYVLHFLGLKPWLCFRDYDCNWNSLVMRDFASDVAHNRWWKVHDKMPRKLQSYCLLRSRQKAGLEWDRRRAEKANFEDGHWQRKITDPRLKICIDKFCFWESMLWHWGETNQTKSLPVPVTPAVSLSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DAI5</accession>
    <name>A0A2K2DAI5_BRADI</name>
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    </protein>
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        <name evidence="1" type="ORF">BRADI_2g25687v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
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            <taxon>Poaceae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="1">Bd21</strain>
        </source>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
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    </dbReference>
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            <dbReference type="EMBL" id="PNT71287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="195" mass="22441" checksum="89CCC606633BFB48" modified="2018-03-28" version="1">MSRFDEIVYLRDCVRETERQLLALRGEVAVWKSKAHATATREEFLLGELVSISSDLYEIIPESHEEAKRVRHRLNTLQRAGPTVPSFCFDSGRGYVLALLQDRAARAKSCLTSCRQIMTDIHRALFPNMPVLDILRGLVRLFTVGRAFVRLRYPGLDLGGLHVVPFFEDGKINMEYYYDAVEWHARELVNLVEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DBE3</accession>
    <name>A0A2K2DBE3_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">BRADI_2g31880v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_024459640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DBE3"/>
    <dbReference type="EnsemblPlants" id="PNT71600">
        <property type="protein sequence ID" value="PNT71600"/>
        <property type="gene ID" value="BRADI_2g31880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112270938"/>
    <dbReference type="Gramene" id="PNT71600">
        <property type="protein sequence ID" value="PNT71600"/>
        <property type="gene ID" value="BRADI_2g31880v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="661484at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36138:SF9">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36138">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="214" mass="23813" checksum="175F7258B592377D" modified="2018-03-28" version="1">MGDARSRFLRSAKFKPHSLDQTGAKADRPAASQVLEHKSLPIPAGSRIGCAPDFSIIAAGHLWRRHEYAKMATDAKTEEKGMNVEKRRKTKKTFMVPQHQLDEILGYKVRPVPPSLAEQNPSRAAKIEKLNEIIVAKQEKFRREYEAQGYATFEAEVTDDEQEKEANPSAASSIEEDANPSAASSIEEDANPSAAFSIPQGRRGRRRFPPARES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DC48</accession>
    <name>A0A2K2DC48_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g36584v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DC48"/>
    <dbReference type="EnsemblPlants" id="PNT71866">
        <property type="protein sequence ID" value="PNT71866"/>
        <property type="gene ID" value="BRADI_2g36584v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71866">
        <property type="protein sequence ID" value="PNT71866"/>
        <property type="gene ID" value="BRADI_2g36584v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DC48"/>
    <dbReference type="OrthoDB" id="678620at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT71866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15744" checksum="AFB919918AEEF6E0" modified="2018-03-28" version="1">MDAPALEVLVSTQCPRLRKLSVSVTLVPASGISLRSATLQHLKFHVPTPRLDIAAPALRVLDTLHGCDDAHIAAPNLAEVTWGLCDSFVFADAGRHLRRLDLTSCTRAAMARLVRRFDLVDELRIPAPLEGDKFWRFSCCNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGD1</accession>
    <name>A0A2K2DGD1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g57288v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGD1"/>
    <dbReference type="EnsemblPlants" id="PNT73343">
        <property type="protein sequence ID" value="PNT73343"/>
        <property type="gene ID" value="BRADI_2g57288v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73343">
        <property type="protein sequence ID" value="PNT73343"/>
        <property type="gene ID" value="BRADI_2g57288v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DGD1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT73343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="91" mass="10400" checksum="EF1A7E17438D4D1C" modified="2018-03-28" version="1">MSASFLFSLSRRLLFIELYRVLNRGIVGSSVCLRLVPADGSFSRRRNRRSIALNASISAPRRAGRLLDPFPLLFFRSPSFFFLSCCRSLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DHF2</accession>
    <name>A0A2K2DHF2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_2g62620v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DHF2"/>
    <dbReference type="FunCoup" id="A0A2K2DHF2">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DHF2"/>
    <dbReference type="EnsemblPlants" id="PNT73703">
        <property type="protein sequence ID" value="PNT73703"/>
        <property type="gene ID" value="BRADI_2g62620v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73703">
        <property type="protein sequence ID" value="PNT73703"/>
        <property type="gene ID" value="BRADI_2g62620v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DHF2"/>
    <dbReference type="OrthoDB" id="616075at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DHF2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011684">
        <property type="entry name" value="NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056889">
        <property type="entry name" value="NET2A-D/KIP1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056888">
        <property type="entry name" value="NET2A-D/KIP1-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31631:SF5">
        <property type="entry name" value="OS01G0976500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31631">
        <property type="entry name" value="PROTEIN NETWORKED 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07765">
        <property type="entry name" value="KIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25014">
        <property type="entry name" value="NET2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24918">
        <property type="entry name" value="NET2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51774">
        <property type="entry name" value="NAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAB" evidence="4">
        <location>
            <begin position="10"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="461"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="660"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="829"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="347"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="621"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="482"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="532"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="672"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT73703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="971" mass="109530" checksum="25F3A23D18E521DD" modified="2018-03-28" version="1">MLQRAASNAYSWWWASHIRTKQSKWLDNHLQAIVFTTGQCNFKNLDMEHRVKCMLLLLGEEADSFSKRAEMYYKRRPEVITQVEEVYRAYRGLADRYDIMSGELHKANHTIATAFPDQVQYAMLEEEDDNIPKAFTPVDPRKIHKSTVDGLMKKKKKGDDPGGVGGAKDTAAPIINKENAREEISRLQKAILVMQTEKEFIKNSYESGIAKYWDLEKEINEMQGQVCHFQDKFDESAVIEDDEARALMTATALKSCEDTILRLQEQRKTSANQALDESERVKVLREKLKPILNEHGKSLPDLSDKSLRKNHVTEMGDVYHVKLGELEMETIVGKIKEHFERDCNISMSDITEQIDELVNKVVDLELMVSSQTSQIDRMRRENDELENTVKSLEEENPDLVSGSSELDEKLKQVEEELIRVQALESSFHKDESTVRSNFTEAISRFSDFSDMLQSPVCEHQAGAMHTSEEAHPSEPCTPDCDDGIKEEEGPQEQNEEKKDTVMADHSSEEAHEPHAVPASHDHTAAPDDVANKTEAAAAAEAAASSDTSADGSKSSDNGQDNKILRPGSLARLRHISSDNQGEEQEGANKQGSVDGAGDMKKLQERLMDGLEDKEKVLLTEYTSLLEDYKDAKRRLLEVEKKNQECLNEIKSLRDQLLGDKERNCSSGERPGRGHRRTPSYGYHQRRHSLSSISKLIRMGSAVQEAAGNNESNDADSGLEDMRLPTIAEVEKPSPLEEKFRRDIDTLLEENLEFWMKFSSSLQRVQEFQSKYDDLQRVTVADNSDGEKKLRALKTEVQVWSEQNAMLRGELQCRFTSLCDIQEEITAALENQEAGEKEEEEEEEGAPRFTSYSAGKFQGEVLNMQQENNRVSDELQSGLDHVKTLQSEIEKKLNVGLSSSSLASGSAEESLDPNASAVVVLARVPSKAKVPLQSFLFPTKAKKPSLFARVTPAMLHKQQVDMKFLARLPRPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKY2</accession>
    <name>A0A2K2DKY2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g24475v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DKY2"/>
    <dbReference type="EnsemblPlants" id="PNT74921">
        <property type="protein sequence ID" value="PNT74921"/>
        <property type="gene ID" value="BRADI_1g24475v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74921">
        <property type="protein sequence ID" value="PNT74921"/>
        <property type="gene ID" value="BRADI_1g24475v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DKY2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT74921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="96" mass="11068" checksum="569C3C4515810BBB" modified="2018-03-28" version="1">MKHYLVVQVTPILQENYSQCSIWNQVFPSKACLHEASELMSRRSCGVVHRRPSHGCPSSVLMKLNQAKRQGETYDSRCSPQVLQDSVCRRRLEFDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DLT1</accession>
    <name>A0A2K2DLT1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g29022v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DLT1"/>
    <dbReference type="EnsemblPlants" id="PNT75242">
        <property type="protein sequence ID" value="PNT75242"/>
        <property type="gene ID" value="BRADI_1g29022v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75242">
        <property type="protein sequence ID" value="PNT75242"/>
        <property type="gene ID" value="BRADI_1g29022v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DLT1"/>
    <dbReference type="OrthoDB" id="696190at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="60"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="183" mass="21111" checksum="BAD088FF1415C137" modified="2018-03-28" version="1">MRTVREELDGAWIADVGPDLWGEALQEFFTLWDWTRDLLLDDSVPDSLTWSWSKGDCYTAKSVYLNLFAGRAEDPMAKELTRRGLQHPPRCPLCDQEMETICHLLLGCVVARKVWTSLLSSRGHADWIPEADSRLRDWWTSLPLPCQTRKDFRTAIILVLWTIWRHRNDVVFNGASLAAARIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DR60</accession>
    <name>A0A2K2DR60_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g53273v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DR60"/>
    <dbReference type="EnsemblPlants" id="PNT76774">
        <property type="protein sequence ID" value="PNT76774"/>
        <property type="gene ID" value="BRADI_1g53273v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76774">
        <property type="protein sequence ID" value="PNT76774"/>
        <property type="gene ID" value="BRADI_1g53273v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DR60"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="72" mass="7983" checksum="A168AD5D8940096D" modified="2018-03-28" version="1">MRSVNDNLHEANAAIHARRRAAAGHELCLGSCISRISAIYELHRDREGRTSISICALLVSLFFFLAMKGDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLI7</accession>
    <name>I1GLI7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100839070</name>
        <name evidence="10" type="ORF">BRADI_1g03640v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563572.1">
        <property type="nucleotide sequence ID" value="XM_003563524.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLI7"/>
    <dbReference type="FunCoup" id="I1GLI7">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GLI7"/>
    <dbReference type="EnsemblPlants" id="KQK12421">
        <property type="protein sequence ID" value="KQK12421"/>
        <property type="gene ID" value="BRADI_1g03640v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839070"/>
    <dbReference type="Gramene" id="KQK12421">
        <property type="protein sequence ID" value="KQK12421"/>
        <property type="gene ID" value="BRADI_1g03640v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839070"/>
    <dbReference type="eggNOG" id="ENOG502QR06">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027462_0_1_1"/>
    <dbReference type="OMA" id="ANDPEWC"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GLI7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009828">
        <property type="term" value="P:plant-type cell wall loosening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.760:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093910" description="Expansin" evidence="7">
        <location>
            <begin position="28"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="56"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="182"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK12421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="266" mass="28834" checksum="7BDB2407C8AE38C5" modified="2012-06-13" version="1">MPSPPLPFFAVLLASVLLLLLPHAADARIPGVYTGGDWQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNNAALSTALFNGGQRCGACFEIKCVNQPDWKWCHPGSPSILVTATNFCPPNYALPSDDGGWCNPPRPHFDLAMPMFLHIAEYRAGIVPVTYRRVACRKKGGVRFTMHGFRYFNLVLITNVAGAGDLVRASVKGSSTGWMPMSRNWGQNWQSNSVLVGQSLSFRVTSSDRRTSTSWNVAPGGWYFGQTFEGKNFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMK4</accession>
    <name>I1GMK4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WPP domain-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100845931</name>
        <name evidence="3" type="ORF">BRADI_1g06570v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558204.1">
        <property type="nucleotide sequence ID" value="XM_003558156.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMK4"/>
    <dbReference type="STRING" id="15368.I1GMK4"/>
    <dbReference type="EnsemblPlants" id="KQK12880">
        <property type="protein sequence ID" value="KQK12880"/>
        <property type="gene ID" value="BRADI_1g06570v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845931"/>
    <dbReference type="Gramene" id="KQK12880">
        <property type="protein sequence ID" value="KQK12880"/>
        <property type="gene ID" value="BRADI_1g06570v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845931"/>
    <dbReference type="eggNOG" id="ENOG502QPVR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021230_0_0_1"/>
    <dbReference type="OMA" id="IKIPAIC"/>
    <dbReference type="OrthoDB" id="619142at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GMK4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037490">
        <property type="entry name" value="WAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33883">
        <property type="entry name" value="WPP DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33883:SF6">
        <property type="entry name" value="WPP DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="576"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK12880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="713" mass="81541" checksum="F24DEA6CE9D20AA3" modified="2012-06-13" version="1">MEALLPVKSDPITRALSERMNPLNRPTFPDGWSITSEDSPAMSPRLLSFKSNSSTESYFQGSGRVLSQSDRHICTPNVDINYAEYLDLMKLEVNAQLDKLKGDVTGLENYALPDNGYIMGTHLGMSLDVMLIEIDERFNALKLLLAVVFRQAREMLGLTSPSVTDLQWEHELQLEVVSITIGECISGLQEELERKLYEQMSVTNTMSKNWQEVVAQFAAMREDLGAVSKLLLPSVPESHISHSKSESSGNRSSRWKYNLFGKKTKEEHSSSRSEGSKSFRKQKSMVISEKSDFRHLNAMTKEETISYFKSEISKLKRTHELALQEKTEELFKLKREKGSLALQNDVEFEPLRRKIPEIITRMDKIISKNIKVPALRETHTDKCRLASRIDSLYYDNQHLRGLLADNMNDVKVLSSQLSEASRDMSLQFSSEEELLRKIEKSKDECEDLLIEGVVKDGLYRTVTGSLLNDSMHEMHDAALNFDAKLFSLEAVISEKEKALCLSNEENWKLKEKLAALEKECLFRDHQEDPEVIKQESVEMILCDIEVEPQTSPGISHEYVELIKLNRNMDYDGALSKKEQEKQLECILISIMKLSKEFVEIEQKLSTERTENRSKDLSDHCSHMVRQAMVLTKISLWYKQMLETRRSELQKAEAKVVILGDKVNAHLGLLQKMHVTLDHYSPTLQQYPGLLDTFLKTCKLVAGLRSRQDEDDAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNI1</accession>
    <name>I1GNI1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100834051</name>
        <name evidence="3" type="ORF">BRADI_1g09240v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559445.1">
        <property type="nucleotide sequence ID" value="XM_003559397.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNI1"/>
    <dbReference type="FunCoup" id="I1GNI1">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GNI1"/>
    <dbReference type="EnsemblPlants" id="KQK13302">
        <property type="protein sequence ID" value="KQK13302"/>
        <property type="gene ID" value="BRADI_1g09240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834051"/>
    <dbReference type="Gramene" id="KQK13302">
        <property type="protein sequence ID" value="KQK13302"/>
        <property type="gene ID" value="BRADI_1g09240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834051"/>
    <dbReference type="eggNOG" id="ENOG502QV14">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066743_0_0_1"/>
    <dbReference type="OMA" id="GCAVVMK"/>
    <dbReference type="OrthoDB" id="1912077at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005134">
        <property type="entry name" value="UPF0114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31721:SF3">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31721">
        <property type="entry name" value="OS06G0710300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03350">
        <property type="entry name" value="UPF0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK13302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="322" mass="34891" checksum="EBA2594DC7B709CA" modified="2012-06-13" version="1">MAAGAARLLRATPAEAIQHPDIRRLPSSSSSPTTSGHVTTSCRPCRIPSLKFPPFLWETTKTRPHGVVSRAAEQRAALITLGSAAPDTTQLEKKRRGLLRLPGDAGSADLLLPLAYEVARRLVLRQFWATRLALLTHRCWAKVAEAVIHQVVVRCQSFTLIGVAGSLVGSVPCFLEGCGVVLESFLLQFRAMSQVVDQAEIIKLLIEALDMFLIGTAMLTFGMGMYFMFYGSRTAQNPIHQRLKEGARFQSIVQAKSRFGHAILLLLQAGVLEKFKSVPLVTGLDMACFAGAVLASSAGVFLLSKMAMHPQRPKPAAFAVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSA5</accession>
    <name>I1GSA5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100822825</name>
        <name evidence="14" type="ORF">BRADI_1g21300v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562679.1">
        <property type="nucleotide sequence ID" value="XM_003562631.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317558.1">
        <property type="nucleotide sequence ID" value="XM_024461790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSA5"/>
    <dbReference type="FunCoup" id="I1GSA5">
        <property type="interactions" value="242"/>
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    <dbReference type="STRING" id="15368.I1GSA5"/>
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        <property type="protein sequence ID" value="KQK15213"/>
        <property type="gene ID" value="BRADI_1g21300v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK15215">
        <property type="protein sequence ID" value="KQK15215"/>
        <property type="gene ID" value="BRADI_1g21300v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822825"/>
    <dbReference type="Gramene" id="KQK15213">
        <property type="protein sequence ID" value="KQK15213"/>
        <property type="gene ID" value="BRADI_1g21300v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15215">
        <property type="protein sequence ID" value="KQK15215"/>
        <property type="gene ID" value="BRADI_1g21300v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822825"/>
    <dbReference type="eggNOG" id="KOG0022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_14_0_1"/>
    <dbReference type="OMA" id="HICEGDA"/>
    <dbReference type="OrthoDB" id="417550at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GSA5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051903">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000007">
        <property type="entry name" value="Alcohol dehydrogenase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF10">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="13">
        <location>
            <begin position="36"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="210"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK15213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="43018" checksum="BBEAC6569FE65093" modified="2012-06-13" version="1">MEQSTAKKPIRCKAAVSKVAGQPLEMVEVDVAPPRAHEVRIRILCTSLCHTDVTFWRMKDFPAMYPSILGHEAAGVVESVGEEVVEVSPGDTVVPVFSGQCGDCPDCLSDRSNICSGLPFRFLPGMPRDGTTRFAFAGTGEPIHNFINVSSFVEYTVVDVAHLVRLGPGLMIPPDKACLLSCGVSTGVGAAWKVAAVEPGSTVAVFGLGAVGLAVAQGSKMRGAKRIIGVDLNPDKCEIGKRMGITEFINPNDTGGKAVSEVIKEMTGGGADYCFECIGSTAVTAEAFSSSRMGWGKTIVLGVASGAAAPISIPSYEILRGRSVVGSLFGGLKPKTDVPLLAQKYLDEELELDEFVTHEMGFDDINGAFDLLTQGKCLRCIIWMDGASAKENGNGGVNVKPKQNGTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSY1</accession>
    <name>I1GSY1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">U-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100835388</name>
        <name evidence="5" type="ORF">BRADI_1g23270v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562806.1">
        <property type="nucleotide sequence ID" value="XM_003562758.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSY1"/>
    <dbReference type="STRING" id="15368.I1GSY1"/>
    <dbReference type="EnsemblPlants" id="KQK15496">
        <property type="protein sequence ID" value="KQK15496"/>
        <property type="gene ID" value="BRADI_1g23270v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835388"/>
    <dbReference type="Gramene" id="KQK15496">
        <property type="protein sequence ID" value="KQK15496"/>
        <property type="gene ID" value="BRADI_1g23270v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835388"/>
    <dbReference type="eggNOG" id="KOG0167">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050445_0_0_1"/>
    <dbReference type="OMA" id="TIFRTDC"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000308">
        <property type="entry name" value="U-box domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000322">
        <property type="entry name" value="U-box domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF129">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="4">
        <location>
            <begin position="193"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="219"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK15496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="473" mass="48560" checksum="F76C81FEEF41FB1F" modified="2012-06-13" version="1">MVSLAAAGSQILSPKQSPSCASSSSTAAAARRPGYSMRTIRSALLHPDSPPGPGSPLRVAAADEGGDSDMENLTDSVIDFHLSELAATAGPAHPAAVAKSSSANAAATEMLELSRDFSDYSSFNSDISGELERLAAAAATATPRSDAPERLAAVDLNDLESMDLSVDAAPLERVEPFVLACVQALGPDAAPDARRTAAARIRLLAKHRSDIRELVGVSGAIPALVPLLRSTDPVAQENAVTALLNLSLEERNRSAITAAGAIKPLVYALRTGTASAKQNAACALLSLSGIEENRATIGACGAIAPLVSLLSAGSTRGKKDALTTLYRLCSARRNKERAVSAGAVLPLVLLIGERGTGTSEKAMVVLASLASIAEGRDAVVEAGGIPALVETIEDGPAREKEFAVVALLQLCSECSSNRALLVREGAIPPLVALSQSGSARAKHKAETLLGYLREQRQGGGCRVGPVAATSMAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTC5</accession>
    <name>I1GTC5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100842830</name>
        <name evidence="2" type="ORF">BRADI_1g24410v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562918.1">
        <property type="nucleotide sequence ID" value="XM_003562870.4"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK15693"/>
        <property type="gene ID" value="BRADI_1g24410v3"/>
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    <dbReference type="GeneID" id="100842830"/>
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        <property type="protein sequence ID" value="KQK15693"/>
        <property type="gene ID" value="BRADI_1g24410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842830"/>
    <dbReference type="eggNOG" id="ENOG502S86B">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_158239_0_0_1"/>
    <dbReference type="OMA" id="ATHAQYM"/>
    <dbReference type="OrthoDB" id="1931827at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034565">
        <property type="entry name" value="Put_cell_wall"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36733">
        <property type="entry name" value="CELL WALL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36733:SF1">
        <property type="entry name" value="CELL WALL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094037" evidence="1">
        <location>
            <begin position="26"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK15693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="103" mass="10742" checksum="8E9694083E643436" modified="2012-06-13" version="1">MAKSASSLLILAVVVAAACFGAAAAARGVPAEKKDEVKRPETIQEGTVLIPGIGRYELGTHYRPDIGGLDHSIPAAAHGQFMPGADDTWVPNPGFEVPNPFHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1GU17</accession>
    <name>I1GU17_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100826113</name>
        <name evidence="7" type="ORF">BRADI_1g26410v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563035.1">
        <property type="nucleotide sequence ID" value="XM_003562987.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GU17"/>
    <dbReference type="FunCoup" id="I1GU17">
        <property type="interactions" value="642"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16042">
        <property type="protein sequence ID" value="KQK16042"/>
        <property type="gene ID" value="BRADI_1g26410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826113"/>
    <dbReference type="Gramene" id="KQK16042">
        <property type="protein sequence ID" value="KQK16042"/>
        <property type="gene ID" value="BRADI_1g26410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826113"/>
    <dbReference type="eggNOG" id="KOG0522">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028500_0_0_1"/>
    <dbReference type="OMA" id="NYPLHWL"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANKRD13"/>
    </dbReference>
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        <property type="entry name" value="ANKRD13_C"/>
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        <property type="entry name" value="Ankyrin_rpt"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12447">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12447:SF7">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11904">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Ankyrin repeat" evidence="6">
        <location>
            <begin position="213"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="381"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="586"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="600"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK16042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="611" mass="66241" checksum="953A40B5C1FAADE8" modified="2012-06-13" version="1">MSVEDPSSAPIPPAGAGAGAARPKRTSSAPIRPSDYAHSPAHHCVALRDAAGLSAILSGLPPLAHPSRILSAADASREARLAASVSAALDRRDVPGGDTALHLAVRLRLPSHASALAAAGADPTLQNHAGWTPLQEALCLGCKDIAPSLLRAHRLAAWAKLRRRAPALSAALRRVQDFYLEVDFHFESSVVPLLSRAAPSDTYRIWKRGADLRADTTLAGFDGLRIRRADHSFLFFGEEAEAGGRRLPPGSLLVLHRGKREVHDAFAAAAAAGDEDAATSDAAAYRPGLNITSARLVPRTTWLRKEKTESVGEWKARVFDVHNVVFSFRTLKAANAGRKDFTFELAGDDEGEEFLPLEIRDDDEDGDFLVADIPPAPPRRSCYVPGRRSVAGPPSHMGTPQRRRNSVDMPRRLPTCASVGRGEDGLFGRHATTGGAKWKEEETVKTLRPTVWLTEDFPLSVDEFLPLLDILATRVRAVRRLRELLTTKFPTGTFPVKVAIPVVPTVRVVITFTKFVPLVEPEEFFTPMSSPSLLASPGPGSIMPKPDTHRSSYLRWASKNSRAKPVNLSQVADNTDPFTIPSDYTWVNLGSSKSQDKKSSKSGKKGKSRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1GUH2</accession>
    <name>I1GUH2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RecA family profile 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100827134</name>
        <name evidence="12" type="ORF">BRADI_1g27880v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RecA family. RAD51 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003563075.4"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010240110.3"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="15368.I1GUH2"/>
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        <property type="protein sequence ID" value="KQK16266"/>
        <property type="gene ID" value="BRADI_1g27880v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16267">
        <property type="protein sequence ID" value="KQK16267"/>
        <property type="gene ID" value="BRADI_1g27880v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16268">
        <property type="protein sequence ID" value="KQK16268"/>
        <property type="gene ID" value="BRADI_1g27880v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16269">
        <property type="protein sequence ID" value="KQK16269"/>
        <property type="gene ID" value="BRADI_1g27880v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16270">
        <property type="protein sequence ID" value="KQK16270"/>
        <property type="gene ID" value="BRADI_1g27880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827134"/>
    <dbReference type="Gramene" id="KQK16266">
        <property type="protein sequence ID" value="KQK16266"/>
        <property type="gene ID" value="BRADI_1g27880v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16267">
        <property type="protein sequence ID" value="KQK16267"/>
        <property type="gene ID" value="BRADI_1g27880v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16268">
        <property type="protein sequence ID" value="KQK16268"/>
        <property type="gene ID" value="BRADI_1g27880v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16269">
        <property type="protein sequence ID" value="KQK16269"/>
        <property type="gene ID" value="BRADI_1g27880v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16270">
        <property type="protein sequence ID" value="KQK16270"/>
        <property type="gene ID" value="BRADI_1g27880v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827134"/>
    <dbReference type="eggNOG" id="KOG1433">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="QRIHTFR"/>
    <dbReference type="OrthoDB" id="336321at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GUH2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:single-stranded DNA binding"/>
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        <property type="term" value="P:DNA strand invasion"/>
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    </dbReference>
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        <property type="term" value="P:double-strand break repair via homologous recombination"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Rad51D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA repair protein RAD51 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051988">
        <property type="entry name" value="HRR_RAD51_Paralog"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047323">
        <property type="entry name" value="Rad51D_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46457">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46457:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="11">
        <location>
            <begin position="28"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007095"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK16269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="262" mass="28352" checksum="5BBC8CB1AD8AC5CE" modified="2012-06-13" version="1">MNEDQPSVENGCVLWSNGMDLFKDAAERNNFLPTGLEGIDMLLGGGLRKGHLTEITGQSSSGKTQVCLYSASHVAARHMGVVLYLDTSNSFSPSRIAHIVDELPISLIKEPKDMRLKRVMSSIVCESVFDVFALFEVLDQLEASMNDKVNSGGNKICLLIIDSVSSLLAPIIGGKNSQGRSMMISVAMILKKLADKHNLSVLVTNHTVGGNGAPKPALGESWKAVPHVRLMISRDHGSNMYTATVLKHTLLASGRHMKFVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1GVD8</accession>
    <name>I1GVD8_BRADI</name>
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        <recommendedName>
            <fullName evidence="5">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100829883</name>
        <name evidence="2" type="ORF">BRADI_1g30390v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563297.1">
        <property type="nucleotide sequence ID" value="XM_003563249.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GVD8"/>
    <dbReference type="STRING" id="15368.I1GVD8"/>
    <dbReference type="EnsemblPlants" id="KQK16753">
        <property type="protein sequence ID" value="KQK16753"/>
        <property type="gene ID" value="BRADI_1g30390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829883"/>
    <dbReference type="Gramene" id="KQK16753">
        <property type="protein sequence ID" value="KQK16753"/>
        <property type="gene ID" value="BRADI_1g30390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829883"/>
    <dbReference type="eggNOG" id="ENOG502R2FM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OMA" id="TNGSEWA"/>
    <dbReference type="OrthoDB" id="440455at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GVD8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd14279">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31245:SF2">
        <property type="entry name" value="OS06G0642650 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31245">
        <property type="entry name" value="UBIQUITIN SYSTEM COMPONENT CUE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK16753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28390" checksum="8589CF63917F6B2A" modified="2012-06-13" version="1">MAAKVAGGRKRGTAAACLDGSGGDDPFFFALKRGRCSPEADVAADLGVSLEFDPVDALQLIFPGADPQLLQGYYEASGNVLDAAIRGFKDYLASGLATTSDDAASSGGTVKSDVPAINTPTNGSEWAELVVKEMYSAKNLVDAKDRAFRILELFDKSTANCNTPDEKHKMHEEHKILKQMLGGLLHQNGVLKRAFLIQHNRLKDYQNMVQERSQFKEIVDKYQQQIKALEDRNYALSFYLEQSKQGNSICGYRNPDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2023-09-13" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I1GVG4</accession>
    <name>I1GVG4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g30570v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GVG4"/>
    <dbReference type="EnsemblPlants" id="KQK16784">
        <property type="protein sequence ID" value="KQK16784"/>
        <property type="gene ID" value="BRADI_1g30570v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16784">
        <property type="protein sequence ID" value="KQK16784"/>
        <property type="gene ID" value="BRADI_1g30570v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1770593_0_0_1"/>
    <dbReference type="InParanoid" id="I1GVG4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094089" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK16784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="15936" checksum="159C412AEA27FA53" modified="2012-06-13" version="1">MQPTRMLAAGLLLLRLLCEASGSSRRLGAAAKLACCGPDLAALRRIRRPKTHPLSASFTGLRFVLCYSSLLRSSAGYVRFFSGSGVRRADPWKAPTHLAPTWPTAASRLRLQLRSLSWPSSPLPHFLAAPLRGSATIGQPRQPPSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I1GX59</accession>
    <name>I1GX59_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g35592v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GX59"/>
    <dbReference type="FunCoup" id="I1GX59">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK17601">
        <property type="protein sequence ID" value="KQK17601"/>
        <property type="gene ID" value="BRADI_1g35592v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17601">
        <property type="protein sequence ID" value="KQK17601"/>
        <property type="gene ID" value="BRADI_1g35592v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SCCZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190956_1_0_1"/>
    <dbReference type="InParanoid" id="I1GX59"/>
    <dbReference type="OMA" id="FHASTRF"/>
    <dbReference type="OrthoDB" id="654716at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33511">
        <property type="entry name" value="OS06G0632400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK17601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="68" mass="7524" checksum="1861B6DFCF247ACA" modified="2012-06-13" version="1">MGGSMSCFGGSGGDGRDYYGEEPRRSSRKVRPSDEDRLWYVGERDVDRKATEFIARFHASASFVDAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYL3</accession>
    <name>I1GYL3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100829887</name>
        <name evidence="6" type="ORF">BRADI_1g42257v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010227694.1">
        <property type="nucleotide sequence ID" value="XM_010229392.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752992.1">
        <property type="nucleotide sequence ID" value="XM_014897506.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYL3"/>
    <dbReference type="STRING" id="15368.I1GYL3"/>
    <dbReference type="EnsemblPlants" id="KQK18395">
        <property type="protein sequence ID" value="KQK18395"/>
        <property type="gene ID" value="BRADI_1g42257v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829887"/>
    <dbReference type="Gramene" id="KQK18395">
        <property type="protein sequence ID" value="KQK18395"/>
        <property type="gene ID" value="BRADI_1g42257v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829887"/>
    <dbReference type="eggNOG" id="KOG0582">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="CSNLPIG"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GYL3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06610">
        <property type="entry name" value="STKc_OSR1_SPAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000099">
        <property type="entry name" value="Serine/threonine-protein kinase BLUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000208">
        <property type="entry name" value="serine/threonine-protein kinase BLUS1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047173">
        <property type="entry name" value="STRAD_A/B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014:SF10">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE FRAY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="440"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="504"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="626"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="653"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK18395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="698" mass="77714" checksum="97A868FF798925AB" modified="2012-06-13" version="1">MEHAPRSRGYPTDPKEYKLYEEVGEGVSATVYRALCVPLNVLVAIKVLDLEKCSNDLDGIRREVQTMSLINHPNLLRACCSFANGHQLWVVMPFMAAGSALHIIKTNFPDGFEESVIATLLWEVLKALVYLHSQGHIHRDVKAGNILIDTNGAVKLGDFGVSACMFDTGNRQRARNTFVGTPCWMAPEVMQQLHGYDYKADIWSFGITALELAHGHAPFSKYPPMKVLLMTLQNAPPGLDYERDKRFSKSFRDLVAACLVKDPQKRPSSEKLLKHSFFKQARSAEFLARSILEGLPPLGDRFRALKAKEADLLLNNKLGPESKEQLSQKEYIRGISGWNFNLEDLKTAAALLDSSNGTYSFNGTNKRDVNGLREAYNEAENVYQERVNHGASARHVEDEIQEVEDLNGELASSFPTRPLEALKSCFDVCGDGILDPTATNLRVQPSAEPISPVQQSSQIEHDRSSNCNGESLERSVSVPSNLGNSVYQKFSSGSLIPEHVLSPYRNVGSDSQRSEFHQRSQSTRNRSGPLLFRQMKDSRPHPSVAPDEPSEGNVVHRRGRFQVTSDNPAQKVAPLASSSRVNLASGVTRPISNSSTVLPTLQFLMQQNTMQKEILSRLISSIEETSDASDASIVGPSQSSGTHSKEKELESYVVNLQRSVTELAEEVQRLKLRNNQLEKQINALSKEDERLRREDTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYP7</accession>
    <name>I1GYP7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100838543</name>
        <name evidence="10" type="ORF">BRADI_1g42556v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560772.1">
        <property type="nucleotide sequence ID" value="XM_003560724.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYP7"/>
    <dbReference type="FunCoup" id="I1GYP7">
        <property type="interactions" value="744"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GYP7"/>
    <dbReference type="EnsemblPlants" id="KQK18454">
        <property type="protein sequence ID" value="KQK18454"/>
        <property type="gene ID" value="BRADI_1g42556v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838543"/>
    <dbReference type="Gramene" id="KQK18454">
        <property type="protein sequence ID" value="KQK18454"/>
        <property type="gene ID" value="BRADI_1g42556v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838543"/>
    <dbReference type="eggNOG" id="KOG4735">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022400_0_0_1"/>
    <dbReference type="OMA" id="SVRMREW"/>
    <dbReference type="OrthoDB" id="2526284at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF56">
        <property type="entry name" value="GALACTAN BETA-1,4-GALACTOSYLTRANSFERASE GALS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="72"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK18454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="502" mass="56432" checksum="7B16FFC4EED82DDB" modified="2012-06-13" version="1">MKPAVKKDAGGVGGGAFGISCLDIKSFAASLALLTLIMALWQLHPCQPLLAITRSPSCPILPLSHTTNATAALPSSTTKLPSSNFTTDRAPPTAAAARPAPLRDPNKRELRPYGSAAALFVQMGAYRGGPRTFAVVGLASKPAFVFNTPYFKCEWVPNPGAGEPVRTKAYKMLPDWGYGRVYTVVVVNCTFPSNPNAGNRGGKLLVHAYYSTKSRRYERFLALEEAPGSYDESRFRPPFPYEYLYCGSSLYGNLSAPRLREWLAYHARFFGPRSHFVLHDAGGVSPEVRAVLDPWVRAGRVTVQDIRAQAEYDGYYYNQFLVVNDCLHRYRHAANWTFFFDVDEYMYLPDGRTLEEVLGQLQRYTQFTIEQNPMSSKLCLEDPAKEYSRKWGFEKLVFRNSITGVRRDRKYAVQARNAYSAGVHMSQNVYGRTTHRTESLIRYYHYHNTINVMGEPCREFVSKPTNGSKVTFEGIPYVYDDNMKRLAGDIKHFEEATVGPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZ94</accession>
    <name>I1GZ94_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DOG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100845860</name>
        <name evidence="3" type="ORF">BRADI_1g44237v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560850.1">
        <property type="nucleotide sequence ID" value="XM_003560802.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZ94"/>
    <dbReference type="STRING" id="15368.I1GZ94"/>
    <dbReference type="EnsemblPlants" id="KQK18712">
        <property type="protein sequence ID" value="KQK18712"/>
        <property type="gene ID" value="BRADI_1g44237v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845860"/>
    <dbReference type="Gramene" id="KQK18712">
        <property type="protein sequence ID" value="KQK18712"/>
        <property type="gene ID" value="BRADI_1g44237v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845860"/>
    <dbReference type="eggNOG" id="ENOG502QW7X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024782_2_2_1"/>
    <dbReference type="OMA" id="CCFKEWM"/>
    <dbReference type="OrthoDB" id="1897224at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051886">
        <property type="entry name" value="Seed_Dev/Stress_Resp_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025422">
        <property type="entry name" value="TGA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46354">
        <property type="entry name" value="DOG1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46354:SF7">
        <property type="entry name" value="PROTEIN DOG1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14144">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51806">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOG1" evidence="2">
        <location>
            <begin position="3"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK18712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="301" mass="32377" checksum="522A17D701049F9D" modified="2012-06-13" version="1">MDMTRYFACHQQWIAGQEAGLGELTAAAANAAARRATEAELRAVVERCLRGYEEYATTRRAMAPEDGAAFFAPPWCTTFEKAVLWLGGCRPSLSIRLLYCVSSEGLEAQLQEFLSGHGRAGGDDMIRPTGSGLLGINAMQLEQINNLHGRTIHEEGILSERLASLQEKIADRPLLPIVREREQERARAAALPRGSASSNGLVGRFAAMGLAGVDAEVDAAMESYTAGLAKLLEEADQLRLSTTRELATEILTPRQAVEMLAAAKQLHLSICDWSRRKEGAQSALLPPAATASSSSSGRPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1H416</accession>
    <name>I1H416_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CSC1-like protein ERD4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100832580</name>
        <name evidence="12" type="ORF">BRADI_1g58380v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557644.1">
        <property type="nucleotide sequence ID" value="XM_003557596.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H416"/>
    <dbReference type="STRING" id="15368.I1H416"/>
    <dbReference type="EnsemblPlants" id="KQK21049">
        <property type="protein sequence ID" value="KQK21049"/>
        <property type="gene ID" value="BRADI_1g58380v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832580"/>
    <dbReference type="Gramene" id="KQK21049">
        <property type="protein sequence ID" value="KQK21049"/>
        <property type="gene ID" value="BRADI_1g58380v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832580"/>
    <dbReference type="eggNOG" id="KOG1134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002458_7_1_1"/>
    <dbReference type="OMA" id="CSCKKEN"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H416">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF100">
        <property type="entry name" value="CSC1-LIKE PROTEIN ERD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="570"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="645"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="5"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="193"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="370"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK21049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="729" mass="81550" checksum="54848F593C605B79" modified="2012-06-13" version="1">MDIASFVTSLLTSFVIFVVLVLVFAWLSRRPGNAPVYYPSVLLRGLDPWEGRGKGTRSPVGWVRQAFSAPEADVIAAGGVDAAVYLVFLSSVLAILVLSGIVLLPVLLPLAATDHALEDPSGSRNGSTSQNFTVIERLALGNVQKKSMRLWAFILSVYWVSFVTYFVLWKSYKHVSNLRAAARSSSDVKPEEFAVLVRDIPVPPPDQTIKDSVDSYFRALHPDTFYKSMVVTDNKEADKIFQEIEGHKQKIAHAEAVYAESKKANKPEGSKPTHRTGFLGLIGKKVDTIEYCNEKIKELLPKLEDEQKNTLQEKQQRAAIIFFNSRAAATSASQTLHAQLFDKWTVTEAPEPREIIWPNLPRKIYDRQIRQSVVYFIVFLTVFFYTIPITAISAVTTLEKLREKLPFLKVVVDQPAIKTVLQAYLPQLALIVFLALLPALLLFLSKSEGIPSQSHVVRAASGKYFYFIIFNVFIGFTISSSLFSALKTIINNPPGIISMLANSLPGSATFFLTFVALKFFVGYGLELSRLVPLIIFHLKKKYLCKTEDEVRAAWAPGDLGYNTRVPNDMLVVTIVLCYSVIAPLIIPFGVAYFALGWLIAKNQVLRVYVPSYESNGRMWPHMHTRVIAALMIYQATMIGVIILKLFYYSTILFPLLAISLIFAYTCHTRFYPAFAKTPLEVACQGLKETPNMGAIYTAYIPPCLKPEKLEDVDIFEDAQSRTTSRAPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4R4</accession>
    <name>I1H4R4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
            <ecNumber evidence="2">2.7.1.150</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">FYVE finger-containing phosphoinositide kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">PIKfyve</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Phosphatidylinositol 3-phosphate 5-kinase type III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC100840550</name>
        <name evidence="23" type="ORF">BRADI_1g60357v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">Bd21</strain>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Plays an important role in maintenance of endomembrane homeostasis including endocytosis, vacuole formation, and vacuolar acidification processes. Required for development of viable pollen. Might mediate recycling of auxin transporters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the PI(3,5)P2 regulatory complex at least composed of ATG18, SAC/FIG4, FAB1 and VAC14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.150" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228521.1">
        <property type="nucleotide sequence ID" value="XM_010230219.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228522.1">
        <property type="nucleotide sequence ID" value="XM_010230220.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312529.1">
        <property type="nucleotide sequence ID" value="XM_024456761.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312530.1">
        <property type="nucleotide sequence ID" value="XM_024456762.1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H4R4"/>
    <dbReference type="EnsemblPlants" id="KQK21360">
        <property type="protein sequence ID" value="KQK21360"/>
        <property type="gene ID" value="BRADI_1g60357v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK21361">
        <property type="protein sequence ID" value="KQK21361"/>
        <property type="gene ID" value="BRADI_1g60357v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK21362">
        <property type="protein sequence ID" value="KQK21362"/>
        <property type="gene ID" value="BRADI_1g60357v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77270">
        <property type="protein sequence ID" value="PNT77270"/>
        <property type="gene ID" value="BRADI_1g60357v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77273">
        <property type="protein sequence ID" value="PNT77273"/>
        <property type="gene ID" value="BRADI_1g60357v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840550"/>
    <dbReference type="Gramene" id="KQK21360">
        <property type="protein sequence ID" value="KQK21360"/>
        <property type="gene ID" value="BRADI_1g60357v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21361">
        <property type="protein sequence ID" value="KQK21361"/>
        <property type="gene ID" value="BRADI_1g60357v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21362">
        <property type="protein sequence ID" value="KQK21362"/>
        <property type="gene ID" value="BRADI_1g60357v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77270">
        <property type="protein sequence ID" value="PNT77270"/>
        <property type="gene ID" value="BRADI_1g60357v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77273">
        <property type="protein sequence ID" value="PNT77273"/>
        <property type="gene ID" value="BRADI_1g60357v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840550"/>
    <dbReference type="eggNOG" id="KOG0230">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000480_4_0_1"/>
    <dbReference type="OMA" id="VGPKFLF"/>
    <dbReference type="OrthoDB" id="158357at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H4R4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009555">
        <property type="term" value="P:pollen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03334">
        <property type="entry name" value="Fab1_TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17300">
        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000007">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000384">
        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase FAB1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000006">
        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase FAB1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044769">
        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF3">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL-3-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="21">
        <location>
            <begin position="36"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="22">
        <location>
            <begin position="1462"/>
            <end position="1785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="331"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="716"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1799"/>
            <end position="1818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1801"/>
            <end position="1818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KQK21360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1818" mass="204796" checksum="359AA5B829931F3E" modified="2012-06-13" version="1">MEASDKTFADVVKLLTSWLPRRSKPDNVSRDFWMPDHSCRVCYDCDTQFTIFNRRHHCRRCGRIFCGKCTAKSVPVRSGPDKSVYEADKIRVCNFCFKQWEQEQTNVFKQMQPVLSPSLSEASLFSTKSTITVNSVSTPAGCYSTGKYQHNMARASNICPPKISQDKASHSTESTHVPEKNMQSISNRDDSSSVQFDYYRNRSDDEDEEYSYFSDRQVQHQQHNNQYFHSDEFDEFDASYNSTISRAIEENVISKELSSYVVDQGLPSTLPITKVEDDPEPDNSSDCGAASSIYALETTDTNPVDFEKNDFFWIPPEPEDVEDEVEADLFDDDYDDDEPVSDGGRWRIRSSSSFGSGEFRSRDRSSEEHKKVMKNVVDGHFRALISQLLQVENIALQEGDDMGWLEIVTSVSWEAANFLRPDTSQGGGMDPGGYVKVKCLAGGHCSESTVVKGVVCKKNVAHRRMTSRIEKPRLLLLAGALEYHRVTNQLSSIDTLLQQETDHLKMAVAKIVAQKPNLLLVENTVSRYAQDLLLEKNISLVLNIKQSLLQRIARCTGAQIVPSIDLLPSQKLGYCELFHVDKHIEHSMTSDNKTKKMLKTMMFFEGCAKPLGCTVLLKGGSMDELKKIKHVVQYGIFAAYHLALETSFLADEGATLPELPLKSPLTVTLPDKRSAADNSISTVPGFTIDISNSQQAIGSFGHLGTDSIMSTDPGRTAVDEPPVDTACPSSPKTNSHSIRPWYTSNIWNDAKLEKDTTSDVLTDHSHIYSTVEKERMYSGDYHDGYLTRSDGKTVRADSTNTNCSYNQNTSVINTNHTSCSNHKEPLDGSIALADVRISNTNDMVVVRPVASPAVQNQETSQGYEITSTKEEVLPSDHQSILVSLSIRCVWKRTICERSQLFRIKYYGNFDKPLGRFLRDYLFDQGYQCRSCDKPPEAHVHCYTHRQGSLTISVRKLPNVVLPGERDGKIWMWHRCLKCPWSDGFPPATQRIVMSDAAWGLSLGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGKMVACFRYAPINVHSVHVPPHKLDFTHQPLDWIQKEANEVIDRAKVLFDEILRSLHVISEKKAHSSSLNVECPNYITDLEGMFRKEKLEFEGCLNKVFKKEAQKCQPDILEINRLRRQLLFHSYLWDQRLRFAARSDRSRHELSNIRQGDKEMIHPVDSFAGPNATDQPQKEISGTEVANKDVKYVEKLQESICRQNCAAVDASNSYCNLDQQIATCESDSLQRSIQTPLYSSVSVNGDTVPLESDLVARRTLSEGQFPSILDVSNALDAKWTGENDPITSKVIVPDSIASSEDSEEHISDTTPSYASVLLNKLGDSAEDHSNWIGMPFLQLYRSLNKQWSRSKRFDALIEYTPVHISFLRAMERQVGPKFLFPIGINDTVVGVYDDEPTSIISYALTSHEYHLQLSDELDRETTETSPSLCDLRSVSLSESIDETSSELLRSVVSAEDNARSISGSKNTSTSDPLLHGKVTHIKVNFGDEGPLEQVKYTVICYYAKQFDALRRICCPSERDFVRSLSRCKKWGAQGGKSNVFFAKSMDDRFIIKQVTKTELESFMKFAPDYFKYLLESIGTGSPTCIAKILGIYQVKSLKGGKETRMDVLVMENLLFERHVTRLYDLKGSTRSRYNPDSNGSDKVLLDENLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLASIDVMDYSLLVGVDEKKHELVMGIIDFMRQYTWDKHLETWVKASGILGGPKNVSPTVISPKQYKKRFRKAMSAYFLVVPDQWSPPAIIPSKQVSESGQDNDQFLSTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9S1</accession>
    <name>I1H9S1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100828535</name>
        <name evidence="2" type="ORF">BRADI_1g75262v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558814.1">
        <property type="nucleotide sequence ID" value="XM_003558766.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H9S1"/>
    <dbReference type="EnsemblPlants" id="KQK23664">
        <property type="protein sequence ID" value="KQK23664"/>
        <property type="gene ID" value="BRADI_1g75262v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828535"/>
    <dbReference type="Gramene" id="KQK23664">
        <property type="protein sequence ID" value="KQK23664"/>
        <property type="gene ID" value="BRADI_1g75262v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828535"/>
    <dbReference type="eggNOG" id="ENOG502QTF3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070206_0_0_1"/>
    <dbReference type="OMA" id="NKVFCNS"/>
    <dbReference type="OrthoDB" id="426136at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018971">
        <property type="entry name" value="DUF1997"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34131">
        <property type="entry name" value="(RAP ANNOTATION RELEASE2) GALACTOSE-BINDING LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34131:SF3">
        <property type="entry name" value="(RAP ANNOTATION RELEASE2) GALACTOSE-BINDING LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09366">
        <property type="entry name" value="DUF1997"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK23664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="232" mass="25407" checksum="417BA94E9BBB1281" modified="2012-06-13" version="1">MAASLRSPPPVPASAFRRSRPGAAVVRASSSSSSSSSTAVSSAPKARFVARRSESTSVQQLARPLAEYMGLPASQYSVLDAERIERVDDSTFRCYVYRFRFFALEVCPVLLVRVDEEPNGCCIRLLSCKLEGSPLVEAQNDKFSASMANRVFCNNSSDGSQQLTSDATIEVTIDIPFPFRAIPVEAIESSGRQVLEQLLRVMLPRFLKQLDKDYQAWASGDSSRKPLGTGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I1HA72</accession>
    <name>I1HA72_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC104581891</name>
        <name evidence="4" type="ORF">BRADI_1g76570v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229266.1">
        <property type="nucleotide sequence ID" value="XM_010230964.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HA72"/>
    <dbReference type="EnsemblPlants" id="KQK23859">
        <property type="protein sequence ID" value="KQK23859"/>
        <property type="gene ID" value="BRADI_1g76570v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581891"/>
    <dbReference type="Gramene" id="KQK23859">
        <property type="protein sequence ID" value="KQK23859"/>
        <property type="gene ID" value="BRADI_1g76570v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104581891"/>
    <dbReference type="HOGENOM" id="CLU_084324_4_0_1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045320">
        <property type="entry name" value="JAGGED/SL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45730:SF108">
        <property type="entry name" value="PROTEIN LATE FLOWERING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45730">
        <property type="entry name" value="ZINC FINGER PROTEIN JAGGED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK23859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="153" mass="16557" checksum="917B5C19E37843CA" modified="2012-06-13" version="1">MEEAKRKAPRVFGHAQAAAELSLSLAPAGSIVQAAAAAVGAPTAARVGGGEERRRLFQCLFCDRTFLKSQALGGHQNAHRWKDRAGGRFRDPYGEEEEPGYYCHGHHEQPGFVHDTSTPRGLEVMGGGHADVERSWPRASGDGGEKLDLQLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAK1</accession>
    <name>I1HAK1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100823956</name>
        <name evidence="14" type="ORF">BRADI_1g77597v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAK1"/>
    <dbReference type="STRING" id="15368.I1HAK1"/>
    <dbReference type="EnsemblPlants" id="KQK24019">
        <property type="protein sequence ID" value="KQK24019"/>
        <property type="gene ID" value="BRADI_1g77597v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK24019">
        <property type="protein sequence ID" value="KQK24019"/>
        <property type="gene ID" value="BRADI_1g77597v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_4_0_1"/>
    <dbReference type="OMA" id="NIMCRIG"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000126">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053062">
        <property type="entry name" value="CYP450_84A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47945">
        <property type="entry name" value="CYTOCHROME P450 84A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47945:SF10">
        <property type="entry name" value="P450 84A1, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="298"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK24019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="537" mass="59671" checksum="C3FFED7BE02984E7" modified="2012-06-13" version="1">MEQFLQEYSSSLVLLLWLALAATLSVPLLRLLLTRRGPPLPPGPWQWPVIGNMLMMGQLTHRGLAALAGRYGGLFHLRLGRVHAVVVSSPAHAREVLGVQDAAFSNRPASAAVAFLTYGRADMAFAHYGRFWRQVRKLSSARLFSRRGSRAARSWLAVRDESAKLVRAIDAEISGSGGGEAAVDIGELLFVLTKNVVFRAAFGDRGRRQEDELGELLREFSELMGAFSVGDFIPWLRWVDGLRGVDERLRKARAGIDELLDRIIDEHLEGKKKSKDDVDADMLDDMLEFFEEDDDDPAAGKKDGGDEPHNDKGLRLTRNNIKAITMDFMFGGMETVAAAMEWAMAELLRSPDNLRRVQQELAETIGLDRTVLDSDINTVPDSLPFLRCIVKETLRLHPPIPLLLHETATDCVVGGYAVPRRSRVIVNLWAIGRDRSAWVDPDTFRPARFMAEAAEVDLKGGSFELLPFGSGRRACPAIVFGLYEMELALARLLHAFEWALPASEVPEDLDMDDVFGLSAPRAVRLRAVPKLRLTCPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJ66</accession>
    <name>I1HJ66_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100845388</name>
        <name evidence="15" type="ORF">BRADI_2g24700v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231346.1">
        <property type="nucleotide sequence ID" value="XM_010233044.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJ66"/>
    <dbReference type="STRING" id="15368.I1HJ66"/>
    <dbReference type="EnsemblPlants" id="KQK06122">
        <property type="protein sequence ID" value="KQK06122"/>
        <property type="gene ID" value="BRADI_2g24700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845388"/>
    <dbReference type="Gramene" id="KQK06122">
        <property type="protein sequence ID" value="KQK06122"/>
        <property type="gene ID" value="BRADI_2g24700v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845388"/>
    <dbReference type="eggNOG" id="KOG2288">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040730_3_0_1"/>
    <dbReference type="OMA" id="MTLLMPP"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HJ66">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000011">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025298">
        <property type="entry name" value="DUF4094"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF275">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13334">
        <property type="entry name" value="DUF4094"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="DUF4094" evidence="14">
        <location>
            <begin position="22"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK06122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="405" mass="44655" checksum="03178A328A804291" modified="2012-06-13" version="1">MMALSERQPQSEKKAPRARPMSAKAVFVLCATSFFVGLLLSGRMTTRLTAPSGSGRGGSGHGSRISLFSDDCEHRRKLEESNNPNDIMNEVSRTHQAIQSLDKSVSSLEMELAVERAKRNGGLGASVSSKGLPKAFVVVGINTAFSSKKRRDSLRDTWVPRGDKLRRLEKEKGIVVRFVIGHSATPGGALDRAIDVEDAETRDFMRLDHVEGYHELSSKTRIYFTAAVATWDAAFYVKVDDDVHVNLGMLTSRLARYRTTPRVYVGCMKSGPVLSQKGVKYHEPESWKFGDEGNKYFRHATGQIYAISRDLASYISINQPILHRFANEDVSLGAWLIGLEVEHIDDRSLCCATPPDCEWKKQAGNVCAASFDWSCSGICKSVDRMRAIHSACGEGDGAVWNFAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1HK06</accession>
    <name>I1HK06_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">signal peptidase I</fullName>
            <ecNumber evidence="5">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100844570</name>
        <name evidence="14" type="ORF">BRADI_2g27080v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S26B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="5"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568566.1">
        <property type="nucleotide sequence ID" value="XM_003568518.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HK06"/>
    <dbReference type="STRING" id="15368.I1HK06"/>
    <dbReference type="MEROPS" id="S26.A04"/>
    <dbReference type="EnsemblPlants" id="KQK06575">
        <property type="protein sequence ID" value="KQK06575"/>
        <property type="gene ID" value="BRADI_2g27080v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844570"/>
    <dbReference type="Gramene" id="KQK06575">
        <property type="protein sequence ID" value="KQK06575"/>
        <property type="gene ID" value="BRADI_2g27080v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844570"/>
    <dbReference type="eggNOG" id="KOG3342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089996_0_2_1"/>
    <dbReference type="OMA" id="ILMNEYP"/>
    <dbReference type="OrthoDB" id="10257561at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000003">
        <property type="entry name" value="Signal peptidase complex catalytic subunit SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF39">
        <property type="entry name" value="SIGNAL PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S24/S26A/S26B/S26C" evidence="13">
        <location>
            <begin position="37"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK06575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="180" mass="20075" checksum="DE4C9AFC6B1FFD3F" modified="2012-06-13" version="1">MGFIGDQVESIRSMQVRHVLSQIISLGMIVTSALIIWKGLMVATGSESPVVVVLSGSMEPGFKRGDILFLRMSKEPIRTGEIVVFNIDGREIPIVHRVIKVHERQESAEVDILTKGDNNFGDDRLLYAQGQLWLHQHHIMGRAVGYLPYVGWVTIVMTEKPIIKYLLIGALGLLVITSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1HM38</accession>
    <name>I1HM38_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Agenet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g36700v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HM38"/>
    <dbReference type="STRING" id="15368.I1HM38"/>
    <dbReference type="EnsemblPlants" id="KQK07633">
        <property type="protein sequence ID" value="KQK07633"/>
        <property type="gene ID" value="BRADI_2g36700v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07633">
        <property type="protein sequence ID" value="KQK07633"/>
        <property type="gene ID" value="BRADI_2g36700v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWQ5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049518_2_0_1"/>
    <dbReference type="InParanoid" id="I1HM38"/>
    <dbReference type="OMA" id="ASARDTW"/>
    <dbReference type="OrthoDB" id="1894168at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HM38">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014002">
        <property type="entry name" value="Agenet_dom_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36805">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36805:SF7">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00743">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Agenet" evidence="2">
        <location>
            <begin position="112"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK07633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="316" mass="35738" checksum="81ADF633F6010B83" modified="2012-06-13" version="1">MDLIIPFKIGDFAEARSFVAGFTGAWFRCKINDIRVVESGRLEYYLEYIDYTEEAKEWTEVFQNNPRDPACHRRKSRASSHMMLRPPFPQWCQGEQNPEHLLNSKLIASARDTWKVGDCVDWFCEGCYWTAKIIRLLSEDEVEVQLLKPPLGEGGVHRVNPKDLRPALDWSMVDGWTVPLSKARGRSWYAVCLIHPKSDIEENTDGEMTSDDDEEEGQNSLRRASNATNCVSRTSNPRDTIMTSTEILKPTSASKSVGNQQGTCRSSKRQASSMEHVPGTVKGAMSEPNGLSNGAGAQQYALRTRKHMNNRSIYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1HRK5</accession>
    <name>I1HRK5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription repressor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ovate family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100823456</name>
        <name evidence="9" type="ORF">BRADI_2g49880v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional repressor that regulates multiple aspects of plant growth and development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567117.1">
        <property type="nucleotide sequence ID" value="XM_003567069.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HRK5"/>
    <dbReference type="STRING" id="15368.I1HRK5"/>
    <dbReference type="EnsemblPlants" id="KQK09737">
        <property type="protein sequence ID" value="KQK09737"/>
        <property type="gene ID" value="BRADI_2g49880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823456"/>
    <dbReference type="Gramene" id="KQK09737">
        <property type="protein sequence ID" value="KQK09737"/>
        <property type="gene ID" value="BRADI_2g49880v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823456"/>
    <dbReference type="eggNOG" id="ENOG502QQT7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_957828_0_0_1"/>
    <dbReference type="OMA" id="CFHKPAS"/>
    <dbReference type="OrthoDB" id="1928390at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038933">
        <property type="entry name" value="Ovate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006458">
        <property type="entry name" value="Ovate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01568">
        <property type="entry name" value="A_thal_3678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057:SF82">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR OFP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR OFP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04844">
        <property type="entry name" value="Ovate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51754">
        <property type="entry name" value="OVATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OVATE" evidence="8">
        <location>
            <begin position="241"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="46"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="151"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK09737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="317" mass="34623" checksum="146688194DCDF44B" modified="2012-06-13" version="1">MRWWSGSGSSNKQQPRDQGQEESKRGKSKAAFSPLAWLSKLTAAKSNNAAASKPKHCAPPAPETKNAGSGFPSFFHKRATPTPSPAASQSSPADSSPAAATTAPEVPLAPRRLSVGNEDAETAAAVRQLCRRRHYSVGGDRDLPPLRHLIPFSRAASPPEPAPTTPMPPLPASDTDEEEEEEKRPRVIIRPRRRCRGGKSERRPSFSGRNPLTPGATRLAVRVRSPRCAVSGSSGLERFAVVRRTSDPQREFRESMVAMIASRRIGRPEELETLLACYLSLNADEHHDCIVKVFRQVWFDLKLKPPRAAAAPARPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HS05</accession>
    <name>I1HS05_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tunicamycin induced 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100837263</name>
        <name evidence="2" type="ORF">BRADI_2g51010v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569901.1">
        <property type="nucleotide sequence ID" value="XM_003569853.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HS05"/>
    <dbReference type="FunCoup" id="I1HS05">
        <property type="interactions" value="794"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HS05"/>
    <dbReference type="EnsemblPlants" id="KQK09932">
        <property type="protein sequence ID" value="KQK09932"/>
        <property type="gene ID" value="BRADI_2g51010v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837263"/>
    <dbReference type="Gramene" id="KQK09932">
        <property type="protein sequence ID" value="KQK09932"/>
        <property type="gene ID" value="BRADI_2g51010v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837263"/>
    <dbReference type="eggNOG" id="ENOG502QQ67">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038643_0_0_1"/>
    <dbReference type="OMA" id="RFTTLWP"/>
    <dbReference type="OrthoDB" id="513870at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HS05">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053283">
        <property type="entry name" value="TUNICAMYCIN_INDUCED_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34454:SF2">
        <property type="entry name" value="PROTEIN TUNICAMYCIN INDUCED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34454">
        <property type="entry name" value="TUNICAMYCIN INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094809" description="Tunicamycin induced 1" evidence="1">
        <location>
            <begin position="27"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK09932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="44657" checksum="C9C91998F2713C71" modified="2012-06-13" version="1">MAAAQARWRFLALPLALFLAIGSSNAAPKPPVPKAISDLREAIVKGLGIQSEELRVSGFDVRDALVGQAVAYEFDMEVGRKAMPVRLLEDVNRWDFVDLPIFRSQADADDTALAEIGRGARGSFEPTLPPFQLAGPMELWIQDGDDVRLALPHDVDAGTLKKVVLADGAVVTVKGARAVSLRLPLELPLPLNRTTYKGRLSSLLSIARALRGAARSNQKPLLSLRIEGPTSLSSTPSMSPNNKLKLKRLAPGQVELSSRAIPAVTEDEDESHSTGLWPLLSLNGSDSSLQGLEELLASVLGKKAGEKGTFKLLNARASAQTYVKMGFTVEKSIADGEVNWSDFPEWKTKPKKLRAHYEVLARVERGQAIPERIAQVQPFQVDEAMSESMLNGNVSRSKVEVVNPPPVYFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSD2</accession>
    <name>I1HSD2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 22">Peroxidase</fullName>
            <ecNumber evidence="5 22">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC100824183</name>
        <name evidence="24" type="ORF">BRADI_2g52077v3</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19 22">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="19 22">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19 22">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="19 22">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 22">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="5 22"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567193.1">
        <property type="nucleotide sequence ID" value="XM_003567145.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSD2"/>
    <dbReference type="FunCoup" id="I1HSD2">
        <property type="interactions" value="195"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HSD2"/>
    <dbReference type="EnsemblPlants" id="KQK10120">
        <property type="protein sequence ID" value="KQK10120"/>
        <property type="gene ID" value="BRADI_2g52077v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824183"/>
    <dbReference type="Gramene" id="KQK10120">
        <property type="protein sequence ID" value="KQK10120"/>
        <property type="gene ID" value="BRADI_2g52077v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824183"/>
    <dbReference type="eggNOG" id="ENOG502R6KP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010543_0_3_1"/>
    <dbReference type="OMA" id="LEDECPM"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000008">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF84">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 22" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 22" id="KW-0349">Heme</keyword>
    <keyword evidence="17 22" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="13 19 22" id="KW-0408">Iron</keyword>
    <keyword evidence="9 19 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 22" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="16" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 22" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982739" description="Peroxidase" evidence="22">
        <location>
            <begin position="27"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="23">
        <location>
            <begin position="31"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="20">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="41"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="74"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="126"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="205"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQK10120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="335" mass="36688" checksum="7539264ABFDB4B92" modified="2012-06-13" version="1">MALDVVLVRLLIIAAVASVLPARGRADGAAGLAIGFYNEKCPGAEDLVLEEMRDIVHEDRTLGPALLRLLFHDCFVRGCDASIMLKSRSKKGERDAKPMSYSLRGFDEVERIKAKLEEACPLTVSCADIIIMAARDAVYLNNGPRFPVETGRRDGKVSNCVDAENDLAPPNANIVDLKTYFSVKNLSWKDLVVLSGSHTIGSSQCAAFAGDRLYNHSGKGMQDPTLNKTYAPDLRMMCEAGNETDTTPVSMDPGSPHEFDLSYYRDVYSNKGLFVSDQALLDDKLTHDYVARMAAAPSPDEFFDDYAAAMINMGRMEVLTGHNGEIRKICGAYVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSM8</accession>
    <name>I1HSM8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DOG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100820986</name>
        <name evidence="14" type="ORF">BRADI_2g52860v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. Binds to the hexamer motif 5'-ACGTCA-3' of histone gene promoters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Binds DNA as a dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564436.1">
        <property type="nucleotide sequence ID" value="XM_003564388.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSM8"/>
    <dbReference type="FunCoup" id="I1HSM8">
        <property type="interactions" value="784"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HSM8"/>
    <dbReference type="EnsemblPlants" id="KQK10238">
        <property type="protein sequence ID" value="KQK10238"/>
        <property type="gene ID" value="BRADI_2g52860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100820986"/>
    <dbReference type="Gramene" id="KQK10238">
        <property type="protein sequence ID" value="KQK10238"/>
        <property type="gene ID" value="BRADI_2g52860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100820986"/>
    <dbReference type="eggNOG" id="ENOG502QU32">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024782_1_3_1"/>
    <dbReference type="OMA" id="FERGQST"/>
    <dbReference type="OrthoDB" id="2015618at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14708">
        <property type="entry name" value="bZIP_HBP1b-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000019">
        <property type="entry name" value="BZIP family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025422">
        <property type="entry name" value="TGA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693">
        <property type="entry name" value="TRANSCRIPTION FACTOR TGA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693:SF48">
        <property type="entry name" value="TRANSCRIPTION FACTOR TGAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14144">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51806">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="12">
        <location>
            <begin position="189"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="DOG1" evidence="13">
        <location>
            <begin position="256"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK10238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="52040" checksum="44278B4A1AB7DA42" modified="2012-06-13" version="1">MESRRGGGPAAAAAEDARGPMPGFGAPQHTIPTDVNIMQTSRVTDFGALAQSAGFRIEDLANLSANTLFNLKPNNHTFISDPLQFGNYGKSISPTDLATTAAAAAAAAITTVDPQALLQQKGVQSNIVALRTRNSENWGESSMADTSPRTDTSTDPDIDVDERNQMFEQGHLAAPTGSDSSDKSRDQLDHKSLRRLAQNREAARKSRLRKKAYIQNLETSRLKLTQLEQELQRARQQGIFISSSGDQSHSTSGNGALAFDMEYARWLEEHNKHINELRAGVNAHAGDDDLRSIVDCIMAHYDEFFRLKGVAAKADVFHVLSGMWKTPAERCFMWLGGFRSSELLKLLAGQLEPLTEQQLAGICNLQQSSQQAEDALSQGMEALQQSLAETLASGSLGPAGSSSNVANYMGQMAMAMGKLGTLENFLRQADNLRLQTLQQMQRILTTRQSARALLAISDYFSRLRALSSLWLARPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUB7</accession>
    <name>I1HUB7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100822835</name>
        <name evidence="5" type="ORF">BRADI_2g57940v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564863.1">
        <property type="nucleotide sequence ID" value="XM_003564815.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUB7"/>
    <dbReference type="STRING" id="15368.I1HUB7"/>
    <dbReference type="EnsemblPlants" id="KQK11070">
        <property type="protein sequence ID" value="KQK11070"/>
        <property type="gene ID" value="BRADI_2g57940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822835"/>
    <dbReference type="Gramene" id="KQK11070">
        <property type="protein sequence ID" value="KQK11070"/>
        <property type="gene ID" value="BRADI_2g57940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822835"/>
    <dbReference type="eggNOG" id="ENOG502QRCE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011924_0_6_1"/>
    <dbReference type="OMA" id="ACEGYTI"/>
    <dbReference type="OrthoDB" id="1861598at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="121"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="321"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="PFYRE" evidence="3">
        <location>
            <begin position="362"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="456"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="272"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK11070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="531" mass="57381" checksum="1A00CAB1F1E31A2A" modified="2012-06-13" version="1">MGMSPSPFTTSYCLTEQDASICTETQELDYHHSYYYGIEDVSLDEVELELAASSGRAHKATKVDYHSSPYHISWPPPPQQASADVESSRVRKKQFRDVLESCKQKVEAMEAMEQHSPPLSGGGVGFQDQQGDSGHGVAVGGEGSSNSGGGTDGMRLVQLLVACAEAVACRDRAQAASLLRELQAGAPVHGTAFQRVASCFVQGLADRLALAHPPALGPASMAFCIPQSSSSASCAGRGEALAVAYEVCPYLRFAHFVANASILEAFEGESKVHVVDLGMTLGLDRAHQWRALLDGLAARGVARPARVRVTGVGARVDAMRAVGLELEAYAEELGMCVEFRAIDRTLESLHVDDLGVEADEAVAINSVLELHCVVKESRGALNSVLQTIRKLAPKAFVLVEQDAGHNGPFFLGRFMEALHYYAALFDALDAALPRYDARRARVEQFHFGAEIRNVVGCEGAARVERHERADQWRRRMSRAGFQSMPIKMAAKAREWLEENAGGTGYTVAEEKGCLVLGWKGKPVIAASCWKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUC6</accession>
    <name>I1HUC6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100824369</name>
        <name evidence="14" type="ORF">BRADI_2g58007v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SKA3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232706.1">
        <property type="nucleotide sequence ID" value="XM_010234404.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUC6"/>
    <dbReference type="STRING" id="15368.I1HUC6"/>
    <dbReference type="EnsemblPlants" id="KQK11085">
        <property type="protein sequence ID" value="KQK11085"/>
        <property type="gene ID" value="BRADI_2g58007v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824369"/>
    <dbReference type="Gramene" id="KQK11085">
        <property type="protein sequence ID" value="KQK11085"/>
        <property type="gene ID" value="BRADI_2g58007v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824369"/>
    <dbReference type="eggNOG" id="ENOG502QWCY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078354_0_0_1"/>
    <dbReference type="OMA" id="VRMNHLV"/>
    <dbReference type="OrthoDB" id="552789at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000940">
        <property type="term" value="C:outer kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033341">
        <property type="entry name" value="SKA3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48118">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48118:SF1">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="12" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="171"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK11085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="309" mass="33470" checksum="7252D5421585AC03" modified="2012-06-13" version="1">MEASISSLCGALSDVLAHADSSSRALSDALSRRPIPLESATSAFLQGLDRRVEAAGADLARLESMAFDTVSVEELLGHFREALGIVSRHADAVESRLTSFGYVAPEVEDEAEDGNEGELEVPENGCFGGSSSVLRSGRERFDDDDALFDDSMSLKNLGFSDACLATLSSQDNDFSASPKISDRKPESVDDAQEITEAEEFIPPEKETDGQDGDSSQGMIRASKEQYEQLPPYMKTLASWEELHDAVSKLNSFFGGAKSEESVALNQDDVGSIGLGRKGRSYLLILLRLNQLTMQTIEGSIFYTLRKSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUW3</accession>
    <name>I1HUW3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100840904</name>
        <name evidence="4" type="ORF">BRADI_2g59770v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565009.1">
        <property type="nucleotide sequence ID" value="XM_003564961.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUW3"/>
    <dbReference type="FunCoup" id="I1HUW3">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HUW3"/>
    <dbReference type="EnsemblPlants" id="KQK11371">
        <property type="protein sequence ID" value="KQK11371"/>
        <property type="gene ID" value="BRADI_2g59770v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840904"/>
    <dbReference type="Gramene" id="KQK11371">
        <property type="protein sequence ID" value="KQK11371"/>
        <property type="gene ID" value="BRADI_2g59770v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840904"/>
    <dbReference type="eggNOG" id="ENOG502QUCE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010441_0_0_1"/>
    <dbReference type="OMA" id="CACKQAH"/>
    <dbReference type="OrthoDB" id="6359943at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HUW3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47369">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47369:SF1">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="69"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK11371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="823" mass="89971" checksum="721F1EB744DED87F" modified="2012-06-13" version="1">MSAAAAAQPHPPAEGSAPAAPLSTQPPSAAAPRPYELAVAAAELRPVDCNLAALCDHIQAEGFGAGSFSDIVVEAMGATYRLHRFILSRSTYFMNMLHGPWREAGAPTVVLHIDDANVDSEAISIALAYLYGQAPKLNDNNAFRVLAAASFLDLQGLCTICTDFIISELWTSNFLQYQLFAESQDYGRHGERVRNACWGYLCQSATLELREVLPKLSSQTLHALLTSDVLWVPNEEKRFELALFALLAKITVSDVEVSGNENLDLMSSSPTVDRSIGKGKSPMNETGEEQLMESELQKLKLHDNMRSNTAHNIIDVPNIIIPQDSIPHSVPQNAEASKRMENDISTGGPSSESTSYQFNENIWLSSEQTKNYYSRTSSSNGLVPAEWGKPTAPIWGGRVVGRRQVRCISGNSSLSADDYNAFMNIFERGSLLYCNMSFDALLSVRKQLEEFEFPCKAINDGLWLQMLLCHRVQAIVADTCRNCCLTSNACACKQAYVSTHSHYRQEHDRSSASGTAGSIYLAEAQGDGNGMFGPVRVNVRGAVDGLAGIGRGNSNVPGAAWAPTRYVFSRVPYGLGSRNGQQSFANDESEPRVDHNGDIPGDGLTALVNLSQESSASHHQAESIFETGMQTGYSSAASVSTPGGSSLQMQESKEHELGSNWESADNTTISLDMRTPLSHFPPFRFGVEFEDVHRLGDGQVKHSAEVFYAGSLWKVSVQAFNDEDPHGRRTLGLFLHRRKAELLLEPTRKAHMYVDPREKVTARYQLICPSKREVMIFGSLKQAGTLLPKAPKGWGWRTAILFDELADLLQGGALRIAAVVQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1HVA6</accession>
    <name>I1HVA6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100822116</name>
        <name evidence="9" type="ORF">BRADI_2g61040v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314274.1">
        <property type="nucleotide sequence ID" value="XM_024458506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HVA6"/>
    <dbReference type="STRING" id="15368.I1HVA6"/>
    <dbReference type="EnsemblPlants" id="KQK11591">
        <property type="protein sequence ID" value="KQK11591"/>
        <property type="gene ID" value="BRADI_2g61040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822116"/>
    <dbReference type="Gramene" id="KQK11591">
        <property type="protein sequence ID" value="KQK11591"/>
        <property type="gene ID" value="BRADI_2g61040v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRJ7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028504_1_0_1"/>
    <dbReference type="OMA" id="HRFINRK"/>
    <dbReference type="OrthoDB" id="1854593at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090447">
        <property type="term" value="F:glycerol-3-phosphate 2-O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010143">
        <property type="term" value="P:cutin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056462">
        <property type="entry name" value="HAD_RAM2/GPAT1-8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15486">
        <property type="entry name" value="ANCIENT UBIQUITOUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15486:SF53">
        <property type="entry name" value="PHOSPHOLIPID_GLYCEROL ACYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23270">
        <property type="entry name" value="HAD_RAM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="8">
        <location>
            <begin position="306"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK11591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="495" mass="53226" checksum="505E6D8B9AAECD96" modified="2012-06-13" version="1">MKGYAHRLLGLHRAVKERLVAGLLRRPSPTTTAAAMAAPVVIETTVAAAILDADALLLAGKTTPAALFPPFFLVAVEAGSFARGLLLLALYPFLHFVKLEACARAMAMVAFCGLRRDEAARIGRAVLPRHFSRSTSIPSPAPEKMTAAVSSSFPTVMVEAFLKEYVGFDAVVGREVRKAGQHYYSGFMEEDDSAEKEARFMAATKGAAEKSKSENNNRGSQQCYPKPMVFHDGRLAFTPTPARALAMYAYLPLAVFLAAVRMAIYTLLPRRLINPAAALAGVRVIVTSSGNNTTSTTSKTGNGGGRLYACNHRTLLDPIAIASALNKPVSAVTYSLSRVSELLSPIPLLRLTRRREEDRARMSALLARGDDVVVCPEGTTCREPYLLRFSPLFAELADEVSPVAVDEKSAVFYGTSTSPGAKCLDSVYFLMNPRPEYSVRFLQPVRTGGGKSSIEVANTVQGAIADALGFQPTAMTRKDKYLLLAGNEGVVPTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1Q3</accession>
    <name>I1I1Q3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100825425</name>
        <name evidence="17" type="ORF">BRADI_3g17330v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>oxalate + O2 + 2 H(+) = H2O2 + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:21880"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
            <dbReference type="EC" id="1.2.3.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 15">Secreted</location>
            <location evidence="2 15">Extracellular space</location>
            <location evidence="2 15">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the germin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573513.1">
        <property type="nucleotide sequence ID" value="XM_003573465.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1Q3"/>
    <dbReference type="SMR" id="I1I1Q3"/>
    <dbReference type="FunCoup" id="I1I1Q3">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ95458">
        <property type="protein sequence ID" value="KQJ95458"/>
        <property type="gene ID" value="BRADI_3g17330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825425"/>
    <dbReference type="Gramene" id="KQJ95458">
        <property type="protein sequence ID" value="KQJ95458"/>
        <property type="gene ID" value="BRADI_3g17330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825425"/>
    <dbReference type="eggNOG" id="ENOG502QQ4A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015790_0_0_1"/>
    <dbReference type="OMA" id="PCKDAMA"/>
    <dbReference type="OrthoDB" id="1850619at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050162">
        <property type="term" value="F:oxalate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0052">Apoplast</keyword>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 12 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094973" description="Germin-like protein" evidence="15">
        <location>
            <begin position="24"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="16">
        <location>
            <begin position="63"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ95458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="225" mass="24276" checksum="BD5DF672478550EC" modified="2012-06-13" version="1">MASSSSFLLLAALLALVSSQVIASDPSPLQDFCVADKNSPVLVNGFVCKNPMYVNADDFFKAAELDKPRMTNKVGSNVTLINVMQIAGLNTLGISIARIDYAPLGQNPPHTHPRATEILTVLEGTLYVGFVTSNPDNKFFSKVLNKGDVFVFPVGLIHFQFNPNPYKPAVAIAALSSQNPGAITIANAVFGSKPPISDDVLAKAFQVEKGTIDWLQAQFWENNHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I1I263</accession>
    <name>I1I263_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g18633v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I263"/>
    <dbReference type="EnsemblPlants" id="KQJ95708">
        <property type="protein sequence ID" value="KQJ95708"/>
        <property type="gene ID" value="BRADI_3g18633v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95708">
        <property type="protein sequence ID" value="KQJ95708"/>
        <property type="gene ID" value="BRADI_3g18633v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174294_0_0_1"/>
    <dbReference type="InParanoid" id="I1I263"/>
    <dbReference type="OMA" id="VFCWCID"/>
    <dbReference type="OrthoDB" id="786260at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095004" description="Dirigent protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ95708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="7320" checksum="1EAEC9DD17B81C93" modified="2012-06-13" version="1">MARALLYPCLLFLASTATASLHLPCLENPSDLTAGGNEVGQVVDNLAGFTAYVTGAVHSNRAIVLASDIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1I343</accession>
    <name>I1I343_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein PHLOEM PROTEIN 2-LIKE A10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100822562</name>
        <name evidence="2" type="ORF">BRADI_3g21530v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573755.1">
        <property type="nucleotide sequence ID" value="XM_003573707.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I343"/>
    <dbReference type="FunCoup" id="I1I343">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I343"/>
    <dbReference type="EnsemblPlants" id="KQJ96208">
        <property type="protein sequence ID" value="KQJ96208"/>
        <property type="gene ID" value="BRADI_3g21530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822562"/>
    <dbReference type="Gramene" id="KQJ96208">
        <property type="protein sequence ID" value="KQJ96208"/>
        <property type="gene ID" value="BRADI_3g21530v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822562"/>
    <dbReference type="eggNOG" id="ENOG502QRNR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038216_0_0_1"/>
    <dbReference type="OMA" id="EMLVAIC"/>
    <dbReference type="OrthoDB" id="1641131at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019141">
        <property type="entry name" value="DUF2045"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21477:SF25">
        <property type="entry name" value="OS10G0102400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21477">
        <property type="entry name" value="ZGC:172139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ96208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="38989" checksum="EBD28534E4D1B5F9" modified="2012-06-13" version="1">MDALAAPTRRSRRLLLAAAAAAASGYGLYRLYLHHRRRVAAVLSLADALSQVGSDLSDFLRSDSDQVPRSLLQLSKLAASDHVSSVASTISESVASGILRALSSSSSRGAARDPSAPPLHDRILDRLFSPAGTGFASAVLGSFARNLVLFSCHDPSSTQPQAGREPEWLAALCSDRGKEAAAELVRVFVATAVAAYLDRTVTVRSSGQVLAGITNPKHEAKLKDLLVSMCNGAVESYVRTSRQVAKEASISRNEDSMLRKVRNSDPSCVMERVSSTLAMPSNRRFVLDVTGRVTAETVRSFLEFSAQRMSDGARKSIVVARDEVTERGLVAVKYLSTKSMAIFTICLAMCMHISVGMRFLLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8E9</accession>
    <name>I1I8E9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100844393</name>
        <name evidence="10" type="ORF">BRADI_3g39850v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in environmental stress response.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574738.1">
        <property type="nucleotide sequence ID" value="XM_003574690.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8E9"/>
    <dbReference type="STRING" id="15368.I1I8E9"/>
    <dbReference type="EnsemblPlants" id="KQJ98899">
        <property type="protein sequence ID" value="KQJ98899"/>
        <property type="gene ID" value="BRADI_3g39850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844393"/>
    <dbReference type="Gramene" id="KQJ98899">
        <property type="protein sequence ID" value="KQJ98899"/>
        <property type="gene ID" value="BRADI_3g39850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844393"/>
    <dbReference type="eggNOG" id="KOG3173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057016_5_0_1"/>
    <dbReference type="OMA" id="YKGVGRD"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4770:FF:000006">
        <property type="entry name" value="Zinc finger A20 and AN1 domain-containing stress-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF75">
        <property type="entry name" value="ZINC FINGER A20 AND AN1 DOMAIN-CONTAINING STRESS-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="A20-type" evidence="8">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="9">
        <location>
            <begin position="106"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="49"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ98899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="18028" checksum="A0811EC1686CB9ED" modified="2012-06-13" version="1">MAQRDKKVEEPTEVQLHAPAEITLCANSCGFPGNPATNNLCQNCFLASSSSSSSTAASPPSPSSSSSSPSAPAPFPLFDKPRPAPSSSAPAAVVVDGPGPAASASRSSVNRCRSCRKRVGLTGFRCRCGEMFCCAHRYSDRHDCSYDYKSAARDAIARENPVVRAAKIVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8L7</accession>
    <name>I1I8L7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_3g40390v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8L7"/>
    <dbReference type="STRING" id="15368.I1I8L7"/>
    <dbReference type="EnsemblPlants" id="KQJ98989">
        <property type="protein sequence ID" value="KQJ98989"/>
        <property type="gene ID" value="BRADI_3g40390v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ98989">
        <property type="protein sequence ID" value="KQJ98989"/>
        <property type="gene ID" value="BRADI_3g40390v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTTS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="I1I8L7"/>
    <dbReference type="OMA" id="YAMEVEV"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I8L7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045009">
        <property type="entry name" value="CASPL-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32021:SF19">
        <property type="entry name" value="CASP-LIKE PROTEIN 5A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32021">
        <property type="entry name" value="CASP-LIKE PROTEIN 5B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="9">
        <location>
            <begin position="36"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ98989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="202" mass="20321" checksum="446C7A6D36A8E409" modified="2012-06-13" version="1">MSHAAIYAMEVEVEGPVAVAAAPPQGVRMDDVPGKSGTPFGLAFRAGQFVCAIIAVAVMSSSDFADIPAFSFLVAASALLCLWSLSMMILDAYALLVKRLLRHYRLFRVITVGDAIIGALLFTAASASGGSSGTYSLQIEMCGTNPCTSYATSTVFAYFSLLALFFTFFLNCSSAAARGAGAAGAAGGGAGAATGAGGAGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8R1</accession>
    <name>I1I8R1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100825623</name>
        <name evidence="3" type="ORF">BRADI_3g40728v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317221.1">
        <property type="nucleotide sequence ID" value="XM_024461453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8R1"/>
    <dbReference type="EnsemblPlants" id="PNT68446">
        <property type="protein sequence ID" value="PNT68446"/>
        <property type="gene ID" value="BRADI_3g40728v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68447">
        <property type="protein sequence ID" value="PNT68447"/>
        <property type="gene ID" value="BRADI_3g40728v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825623"/>
    <dbReference type="Gramene" id="PNT68446">
        <property type="protein sequence ID" value="PNT68446"/>
        <property type="gene ID" value="BRADI_3g40728v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68447">
        <property type="protein sequence ID" value="PNT68447"/>
        <property type="gene ID" value="BRADI_3g40728v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1987">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004253_2_2_1"/>
    <dbReference type="OMA" id="SEMFHAH"/>
    <dbReference type="OrthoDB" id="10261408at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF238">
        <property type="entry name" value="OS08G0523100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="163"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT68446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="223" mass="24106" checksum="3FE996CD2292A44C" modified="2012-06-13" version="1">MVDSGFIEFKLDYQKTRKLALGHKLSKSIRAGEHTLTVDCYPRGLFGGNYPGDDLPLHLFLAIKSKNINLIFEAFVVGKDGAPSPSHAKRTVAFESIVGGGTRHATLFSFLKQSDLESLCAANGGVVTVVCGVILRHDENPITVPAPNIGVHLRDMMGCTDGSDVSFSVGRETFHAHRAMLAARSPVFKAELFGSMAESKLPCVTVCGIEPSIFKALPHFIYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1I940</accession>
    <name>I1I940_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">biotin synthase</fullName>
            <ecNumber evidence="3">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100827697</name>
        <name evidence="16" type="ORF">BRADI_3g41750v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="13">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="3"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574854.1">
        <property type="nucleotide sequence ID" value="XM_003574806.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I940"/>
    <dbReference type="STRING" id="15368.I1I940"/>
    <dbReference type="EnsemblPlants" id="KQJ99204">
        <property type="protein sequence ID" value="KQJ99204"/>
        <property type="gene ID" value="BRADI_3g41750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827697"/>
    <dbReference type="Gramene" id="KQJ99204">
        <property type="protein sequence ID" value="KQJ99204"/>
        <property type="gene ID" value="BRADI_3g41750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827697"/>
    <dbReference type="eggNOG" id="KOG2900">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033172_1_2_1"/>
    <dbReference type="OMA" id="CTERSEQ"/>
    <dbReference type="OrthoDB" id="2414104at2759"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000011">
        <property type="entry name" value="Biotin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="BIOTIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976:SF2">
        <property type="entry name" value="BIOTIN SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01060">
        <property type="entry name" value="BATS_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00272">
        <property type="entry name" value="biotin_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01278">
        <property type="entry name" value="biotin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095139" description="biotin synthase" evidence="14">
        <location>
            <begin position="20"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="15">
        <location>
            <begin position="73"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001619-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQJ99204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="374" mass="41021" checksum="73F2ECE0B6801A76" modified="2012-06-13" version="1">MMLLARSSLRLRLAPMASAAAPFSAAAEAAERAVRDGPRNDWTRPEIQAIYDSPLLDLLFHGAQVHRNVHKFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGLKADRLMKKDAVLEAAKKAKEAGSTRFCMGAAWRETIGRKTNFNQILEYVKDIRGMGMEVCCTLGMLEKQQAEQLKTAGLTAYNHNLDTSREYYPNIITTRSYDDRLQTLQHVREAGISVCSGGIIGLGEAEEDRVGLLHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIASARIVMPKAMVRLSAGRVRFSMPEQALCFLAGANSIFAGEKLLTTANNDFDADQAMFKILGLIPKAPNFGDEEATSAASTESCEQAASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBK0</accession>
    <name>I1IBK0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100825740</name>
        <name evidence="3" type="ORF">BRADI_3g48690v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575351.1">
        <property type="nucleotide sequence ID" value="XM_003575303.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBK0"/>
    <dbReference type="FunCoup" id="I1IBK0">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IBK0"/>
    <dbReference type="EnsemblPlants" id="KQK00329">
        <property type="protein sequence ID" value="KQK00329"/>
        <property type="gene ID" value="BRADI_3g48690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825740"/>
    <dbReference type="Gramene" id="KQK00329">
        <property type="protein sequence ID" value="KQK00329"/>
        <property type="gene ID" value="BRADI_3g48690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825740"/>
    <dbReference type="eggNOG" id="ENOG502S1AC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116500_0_0_1"/>
    <dbReference type="OMA" id="MAWCLSA"/>
    <dbReference type="OrthoDB" id="498786at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35713">
        <property type="entry name" value="ARGININE/SERINE-RICH-LIKE SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35713:SF1">
        <property type="entry name" value="ARGININE_SERINE-RICH-LIKE SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095172" evidence="2">
        <location>
            <begin position="23"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK00329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="139" mass="15306" checksum="3548F9A73277D106" modified="2012-06-13" version="1">MASAWCLLAPAAAPVAAPGALGVSASGGAFVARAAVPARRRCRWDALVVCMAPDEEKITRRSPLDFPIEWEKPKPGRRPDIFPKFSPMKTPLPHPLPADDPLDDDEEEEEEEAQPQEEPQEDDPDKEDPEEDDPDKPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICL9</accession>
    <name>I1ICL9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CAAX prenyl protease</fullName>
            <ecNumber evidence="15">3.4.24.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100821110</name>
        <name evidence="18" type="ORF">BRADI_3g51730v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Proteolytically removes the C-terminal three residues of farnesylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic side chains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.24.84"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14 15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 15">Belongs to the peptidase M48A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.84" evidence="15"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570103.1">
        <property type="nucleotide sequence ID" value="XM_003570055.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICL9"/>
    <dbReference type="STRING" id="15368.I1ICL9"/>
    <dbReference type="EnsemblPlants" id="KQK00774">
        <property type="protein sequence ID" value="KQK00774"/>
        <property type="gene ID" value="BRADI_3g51730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821110"/>
    <dbReference type="Gramene" id="KQK00774">
        <property type="protein sequence ID" value="KQK00774"/>
        <property type="gene ID" value="BRADI_3g51730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821110"/>
    <dbReference type="eggNOG" id="KOG2719">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025947_3_3_1"/>
    <dbReference type="OMA" id="FVIEEKF"/>
    <dbReference type="OrthoDB" id="360839at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ICL9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07343">
        <property type="entry name" value="M48A_Zmpste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.10:FF:000005">
        <property type="entry name" value="CAAX prenyl protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027057">
        <property type="entry name" value="CAXX_Prtase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032456">
        <property type="entry name" value="Peptidase_M48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16491">
        <property type="entry name" value="Peptidase_M48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 15" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 14 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="332"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 1 N-terminal" evidence="17">
        <location>
            <begin position="27"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="16">
        <location>
            <begin position="214"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK00774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="425" mass="48584" checksum="B6FE60B7CFB4CEC4" modified="2012-06-13" version="1">MALPYLEAVLCFMILLYIFETYLNIRQHRALKLPTLPKPLVGVISGEKFERSRAYSLDKSNFNFVREAVTITSDIIILYYKVLPWFWKKSGELATNIGLNAENEIIHTLAFLAGVMVWSQITDLPFSLYSTFVIEARHGFNKQTIWLFIRDMIKGILLSTLLAPPIVSAIIIIVQNGGPYLAIYLWGFMFALALLMMTIYPIMIAPLFNKFTPLPEGTLREKIEKLADSLKFPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCTNENEIVSVLAHELGHWKLNHTAYSFVAVQLLTFLQFGGYTLVRNSKDLFESFGFEDQSVIIGLIIFMHTIIPVQHLLSFCLNLVSRAFEFQADAFAKNLGYAPELRAALVKLQEENLSAMNTDPWYSAYHYSHPPLVERLSAIEDLDSKKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICY3</accession>
    <name>I1ICY3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pectin acetylesterase</fullName>
            <ecNumber evidence="5">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100843877</name>
        <name evidence="6" type="ORF">BRADI_3g52600v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Secreted</location>
            <location evidence="2 5">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the pectinacetylesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235850.1">
        <property type="nucleotide sequence ID" value="XM_010237548.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235851.1">
        <property type="nucleotide sequence ID" value="XM_010237549.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317109.1">
        <property type="nucleotide sequence ID" value="XM_024461341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICY3"/>
    <dbReference type="STRING" id="15368.I1ICY3"/>
    <dbReference type="ESTHER" id="bradi-i1icy3">
        <property type="family name" value="Pectinacetylesterase-Notum"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00906">
        <property type="protein sequence ID" value="KQK00906"/>
        <property type="gene ID" value="BRADI_3g52600v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00907">
        <property type="protein sequence ID" value="KQK00907"/>
        <property type="gene ID" value="BRADI_3g52600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843877"/>
    <dbReference type="Gramene" id="KQK00906">
        <property type="protein sequence ID" value="KQK00906"/>
        <property type="gene ID" value="BRADI_3g52600v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00907">
        <property type="protein sequence ID" value="KQK00907"/>
        <property type="gene ID" value="BRADI_3g52600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843877"/>
    <dbReference type="eggNOG" id="KOG4287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031008_0_3_1"/>
    <dbReference type="OMA" id="LSPGMCF"/>
    <dbReference type="OrthoDB" id="2015280at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ICY3">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052793">
        <property type="term" value="F:pectin acetylesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF93">
        <property type="entry name" value="PECTIN ACETYLESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK00906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="395" mass="43502" checksum="D4051377E4B5E616" modified="2012-06-13" version="1">MANDGSKLRLWCSAFACALAFLGADGFFVDITYVDSAVAKGAVCLDGSAPAYHLARGFGSGVDSWLVHFEGGGWCSNVTTCLERKNTRLGSSKEMAKQVAFSGILSNTPDHNPDFYNWNKVRVRYCDGSSFTGDKEEVDPTTKLHYRGARVWQAVMEDLLAKGMDRAENALISGCSAGGLTSVLHCDRFRDRMPVEANVKCLSDAGFFIDVKDIAGEKHAADFFNDVVTTHGSAKNLPSSCTSKLPPGMCLFPQNEVKQIQTPLFILNAAYDSWQVRNILVPGGSDPHWRSCKHDINQCSEKQLKTLQGFRDDFLKALEEQGSSSSRGLFINSCFAHCQSEIQEIWFAPDSPVLGNKKIANAIGDWFYDRSPFQEIDCPYPCDSSCHVFKNSSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IE67</accession>
    <name>I1IE67_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100844679</name>
        <name evidence="15" type="ORF">BRADI_3g56030v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase A22B family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570436.1">
        <property type="nucleotide sequence ID" value="XM_003570388.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IE67"/>
    <dbReference type="MEROPS" id="A22.A05"/>
    <dbReference type="EnsemblPlants" id="KQK01463">
        <property type="protein sequence ID" value="KQK01463"/>
        <property type="gene ID" value="BRADI_3g56030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844679"/>
    <dbReference type="Gramene" id="KQK01463">
        <property type="protein sequence ID" value="KQK01463"/>
        <property type="gene ID" value="BRADI_3g56030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844679"/>
    <dbReference type="eggNOG" id="KOG2442">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023799_4_1_1"/>
    <dbReference type="OMA" id="IAPVNCP"/>
    <dbReference type="OrthoDB" id="29661at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030660">
        <property type="term" value="C:Golgi-associated vesicle membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000007">
        <property type="entry name" value="Signal peptide peptidase-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF75">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095235" description="PA domain-containing protein" evidence="13">
        <location>
            <begin position="28"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="275"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="14">
        <location>
            <begin position="95"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK01463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="546" mass="59574" checksum="7B95DBC2309471C6" modified="2012-06-13" version="1">MGAGSPETAAVAALLVMAAALAGVAAGGDIVHQDDDAPKIPGCSNDFMLVKVQTWVNNRETDEFVGVGARFGPIIESKEKHANRTGLLQADPFDCCAPLKEKVAGDVLLVRRGGCRFTTKTKIAEDAGASAIIIMNNRHELYKMVCDKNETDLDINIPAVLLPQDAGTILQGLLSLGQVSVQLYSPDRPLVDTAEVFLWLMAVGTILCASYWSAWSARESVIEQEKLLKDGHETSVNFEAGGSSGMVDITMVSAILFIVVASCFLIMLYKLMSHWFVELLVVIFCIGGVEGLQTCLVALLSRWFKPAAGSFVKVPFFGAVSYLTLAVCPFCIVIAVIWAVYRRQPYAWIGQDVLGIALIVTVIQIVRIPNLKVGSVLLGCSFLYDIFWVFISKMWFHESVMIVVARGDKTDEDGVPMLLKIPRMFDPWGGYSIIGFGDILLPGLLVAFALRYDWAAKKTLQSGYFLWSMVAYGSGLLITYVALNLMDGHGQPALLYIVPFTLGTFISLGKKRGELRNLWTRGQPPRICTHTQHPSKESSTAAVTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEL1</accession>
    <name>I1IEL1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100843973</name>
        <name evidence="8" type="ORF">BRADI_3g57210v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM192 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570516.1">
        <property type="nucleotide sequence ID" value="XM_003570468.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEL1"/>
    <dbReference type="FunCoup" id="I1IEL1">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IEL1"/>
    <dbReference type="EnsemblPlants" id="KQK01629">
        <property type="protein sequence ID" value="KQK01629"/>
        <property type="gene ID" value="BRADI_3g57210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843973"/>
    <dbReference type="Gramene" id="KQK01629">
        <property type="protein sequence ID" value="KQK01629"/>
        <property type="gene ID" value="BRADI_3g57210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843973"/>
    <dbReference type="eggNOG" id="ENOG502QT7U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069294_0_0_1"/>
    <dbReference type="OMA" id="ICAGWHI"/>
    <dbReference type="OrthoDB" id="564295at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029399">
        <property type="entry name" value="TMEM192"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31592">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31592:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14802">
        <property type="entry name" value="TMEM192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="306"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK01629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="354" mass="40387" checksum="7DE266FA4CF77E17" modified="2012-06-13" version="1">MSHERGGAASSFAVPASEEDALFIDLLHEAPLSGHREPRSIVSGTLYCIVLAGYAAVTVSAPWIFLLVPDMVPPLLCSSNVILLLLTGIFEQYWVHQVRKVRLQGYYDFSQKLKRIARLPFATIAYGTGLMLLIIVWQPLVQILSVSLLLRIAIVVEAICAGLFMGLYIWYIHKYNSLNGQPDILRSLYSALQPSNTLEDRRYYDGRLSDQQMALLQYQRENIHYLSEEVLRLQECLSKYQRTDVGNTPQVDLAHLLASRDQELRALSAEMNQVHSELHLARGLIDEKDSEIQRIRVSNNQYVEENDRLRAILGEWSARAAKLERALEAERMSNIELRKNIAKFRGQLHKEQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1IHR3</accession>
    <name>I1IHR3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100836056</name>
        <name evidence="1" type="ORF">BRADI_4g05410v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575655.1">
        <property type="nucleotide sequence ID" value="XM_003575607.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IHR3"/>
    <dbReference type="STRING" id="15368.I1IHR3"/>
    <dbReference type="EnsemblPlants" id="KQJ86425">
        <property type="protein sequence ID" value="KQJ86425"/>
        <property type="gene ID" value="BRADI_4g05410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836056"/>
    <dbReference type="Gramene" id="KQJ86425">
        <property type="protein sequence ID" value="KQJ86425"/>
        <property type="gene ID" value="BRADI_4g05410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836056"/>
    <dbReference type="eggNOG" id="ENOG502R3G3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039568_1_0_1"/>
    <dbReference type="OMA" id="WIRDYSA"/>
    <dbReference type="OrthoDB" id="681719at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IHR3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040249">
        <property type="entry name" value="Ricin_B-like_lectin_EULS3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31257:SF5">
        <property type="entry name" value="ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31257">
        <property type="entry name" value="RICIN B-LIKE LECTIN EULS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ86425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="155" mass="17259" checksum="666190D9645FCBC8" modified="2012-06-13" version="1">METEKPMKIFCKMNMSLNAAVRGDKVMLVTADSSDKSQHWIQDYSATGNLTDDDGRRAFALVNRTTGQAMVNINGEGPVQLAPYSGHVAVELNKLWSLGEERDDGFSNVRSLQNTFFTLNAMGGYPVKSGALLGTYRPQEYDHTYWKIVSVNDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IN83</accession>
    <name>I1IN83_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Abscisic stress ripening</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100844019</name>
        <name evidence="3" type="ORF">BRADI_4g24650v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the abscisic acid and water stress-induced protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577811.1">
        <property type="nucleotide sequence ID" value="XM_003577763.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IN83"/>
    <dbReference type="STRING" id="15368.I1IN83"/>
    <dbReference type="EnsemblPlants" id="KQJ89283">
        <property type="protein sequence ID" value="KQJ89283"/>
        <property type="gene ID" value="BRADI_4g24650v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844019"/>
    <dbReference type="Gramene" id="KQJ89283">
        <property type="protein sequence ID" value="KQJ89283"/>
        <property type="gene ID" value="BRADI_4g24650v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844019"/>
    <dbReference type="eggNOG" id="ENOG502S1T2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094741_0_0_1"/>
    <dbReference type="OMA" id="FHHKDKA"/>
    <dbReference type="OrthoDB" id="696887at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IN83">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003496">
        <property type="entry name" value="ABA_WDS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33801">
        <property type="entry name" value="ABSCISIC STRESS-RIPENING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33801:SF24">
        <property type="entry name" value="ABSCISIC STRESS-RIPENING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02496">
        <property type="entry name" value="ABA_WDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ89283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15399" checksum="CA3B59E0B29CC5D2" modified="2012-06-13" version="1">MAEEKKHHLFHHKKEDTDVVVNPTTGAVDEYGYSAETVVTPTGADGEYERITKEEKHHKHKEHLGEMGAVAAGAFALYEKHEAKKDPENAHRHKIAEEVGAAAAVGAGGFVFHEHHEKKKDHKEAQEVSGEKKGHHLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IR64</accession>
    <name>I1IR64_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1664 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100845945</name>
        <name evidence="3" type="ORF">BRADI_4g33460v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578313.1">
        <property type="nucleotide sequence ID" value="XM_003578265.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IR64"/>
    <dbReference type="STRING" id="15368.I1IR64"/>
    <dbReference type="EnsemblPlants" id="KQJ90715">
        <property type="protein sequence ID" value="KQJ90715"/>
        <property type="gene ID" value="BRADI_4g33460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845945"/>
    <dbReference type="Gramene" id="KQJ90715">
        <property type="protein sequence ID" value="KQJ90715"/>
        <property type="gene ID" value="BRADI_4g33460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845945"/>
    <dbReference type="eggNOG" id="ENOG502QRN8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="QDMTNSG"/>
    <dbReference type="OrthoDB" id="544175at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IR64">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1480.30">
        <property type="entry name" value="Designed four-helix bundle protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012458">
        <property type="entry name" value="DUF1664"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46667">
        <property type="entry name" value="OS05G0182700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46667:SF1">
        <property type="entry name" value="OS09G0482740 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07889">
        <property type="entry name" value="DUF1664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1664" evidence="2">
        <location>
            <begin position="92"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ90715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="320" mass="34941" checksum="A08C8EE0719BF840" modified="2012-06-13" version="1">MAMATQTGFAASKVLILVGAGLTGSIVLRNGRLSDVLAELQELMKGVNLGEGSSPYDIALIQAQIRNLAQEVRDLTLSRPITILSGKSDSGGSFSSYILPAAAVGALGYGYMWIKGWSLSDVMFVTKRNMASAVESMSKQLEQVSSALAATKKHLTQRLQNLDGKMDEQVEISKHIRNEVNDVKTDLSQIGFDVEAIQQMVAGLEEKIYLLDNKQDAANAGIWYLCKMAGGIKEGINAKFFQEADEKLKLLDQAQTEKKSVKGLELFLESNEELKAIDSKPNTVTQNDSKKTMTTFDVPVKSAAVHRSNRFSFRKEGLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1ISK0</accession>
    <name>I1ISK0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal protein L18e/L15P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100826705</name>
        <name evidence="5" type="ORF">BRADI_4g37180v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238469.1">
        <property type="nucleotide sequence ID" value="XM_010240167.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758696.1">
        <property type="nucleotide sequence ID" value="XM_014903210.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310852.1">
        <property type="nucleotide sequence ID" value="XM_024455084.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310853.1">
        <property type="nucleotide sequence ID" value="XM_024455085.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310854.1">
        <property type="nucleotide sequence ID" value="XM_024455086.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310855.1">
        <property type="nucleotide sequence ID" value="XM_024455087.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310856.1">
        <property type="nucleotide sequence ID" value="XM_024455088.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310857.1">
        <property type="nucleotide sequence ID" value="XM_024455089.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310858.1">
        <property type="nucleotide sequence ID" value="XM_024455090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ISK0"/>
    <dbReference type="STRING" id="15368.I1ISK0"/>
    <dbReference type="EnsemblPlants" id="KQJ91362">
        <property type="protein sequence ID" value="KQJ91362"/>
        <property type="gene ID" value="BRADI_4g37180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826705"/>
    <dbReference type="Gramene" id="KQJ91362">
        <property type="protein sequence ID" value="KQJ91362"/>
        <property type="gene ID" value="BRADI_4g37180v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826705"/>
    <dbReference type="eggNOG" id="KOG1870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160999_0_0_1"/>
    <dbReference type="OMA" id="FECGRTC"/>
    <dbReference type="OrthoDB" id="736100at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000006">
        <property type="entry name" value="50S ribosomal protein L18-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_uL18_bac/plant/anim"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899:SF21">
        <property type="entry name" value="OS09G0549700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095498" description="Ribosomal protein L18e/L15P domain-containing protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ91362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13570" checksum="11ABC3B91A85A7E1" modified="2012-06-13" version="1">MAQAKRYVLRLFISLKFVTANVVDRQSGRVVVTASSVERPLRDGLECGRACNAKAAAAVGEVLAMRLRVDGLAREPIHANADKEIEKKGFKNRTKVWAILNALRTHGVNLRLDDDGDHRRHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITD3</accession>
    <name>I1ITD3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100842894</name>
        <name evidence="8" type="ORF">BRADI_4g39500v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576929.1">
        <property type="nucleotide sequence ID" value="XM_003576881.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITD3"/>
    <dbReference type="STRING" id="15368.I1ITD3"/>
    <dbReference type="EnsemblPlants" id="KQJ91742">
        <property type="protein sequence ID" value="KQJ91742"/>
        <property type="gene ID" value="BRADI_4g39500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842894"/>
    <dbReference type="Gramene" id="KQJ91742">
        <property type="protein sequence ID" value="KQJ91742"/>
        <property type="gene ID" value="BRADI_4g39500v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842894"/>
    <dbReference type="eggNOG" id="ENOG502S2JP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077246_0_0_1"/>
    <dbReference type="OMA" id="ACQNTWS"/>
    <dbReference type="OrthoDB" id="204175at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040327">
        <property type="entry name" value="At5g14285-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31766">
        <property type="entry name" value="GLABROUS1 ENHANCER-BINDING PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31766:SF5">
        <property type="entry name" value="STOREKEEPER PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="35"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ91742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="252" mass="27724" checksum="7C4AE99F06588762" modified="2012-06-13" version="1">MEAGDSQSMFLPFLLMFASIYLVAYLFLFRRWSRDQRPDASSCLTSLFHGTPAALLALRAVLSNPSFAGGDLALAAPNTAAEDLVLDFSTAYFAVDLLHYLVFLRHETLFIAHHLATLYVFATCRAAARRGAYGLLALEVLAEATSLAQNLWTLAGMRRAQGSPAAARAHAALSLPFYVAYTAMRGVLGPLWFVKMVRFYAAAGRQSGLPAWAWVSWSVVIGAGILLSVLWVANLWLAYFRDRIHSSKTKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVT0</accession>
    <name>I1IVT0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphotransferase</fullName>
            <ecNumber evidence="12">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100835291</name>
        <name evidence="15" type="ORF">BRADI_5g01836v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="12"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580531.1">
        <property type="nucleotide sequence ID" value="XM_003580483.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVT0"/>
    <dbReference type="FunCoup" id="I1IVT0">
        <property type="interactions" value="1210"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IVT0"/>
    <dbReference type="EnsemblPlants" id="KQJ81622">
        <property type="protein sequence ID" value="KQJ81622"/>
        <property type="gene ID" value="BRADI_5g01836v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835291"/>
    <dbReference type="Gramene" id="KQJ81622">
        <property type="protein sequence ID" value="KQJ81622"/>
        <property type="gene ID" value="BRADI_5g01836v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835291"/>
    <dbReference type="eggNOG" id="KOG1369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014393_5_1_1"/>
    <dbReference type="OMA" id="YPNFEGY"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd24020">
        <property type="entry name" value="ASKHA_NBD_HK_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000034">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000033">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF63">
        <property type="entry name" value="HEXOKINASE-LIKE 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="13">
        <location>
            <begin position="43"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="14">
        <location>
            <begin position="249"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQJ81622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="494" mass="53497" checksum="220196578791081C" modified="2012-06-13" version="1">MSAAVGSPFRAAPLAQHRRRRGYAVVRRCSAVEAGCGAAPPILKELRLQCATPLPLLRRVADAMAADMRAGLAADGAGELKMIPSYVYSLPTGNETGLFYALDLGGTNFRVLRVQLGGKDKRVVGTEFEQVSIPKEIMHGTTEELFDFIADRLSAFVAKEGGDFHLAEGRNREIGFTFSFPVKQTSIDSGILIKWTKGFAVSGTAGKDVVACLNAAMERQGLDMRVSALVNDTVGTLAGAHYWDDDVMVAVILGTGTNACYIERTEVIPKLQHLGLGAGNTIINTEWGAFSDNLPLTEFDRAMDIESINPGEQIFEKTISGMYLGEIVRRVLVKMAQVSDLFGHSFPNKLAEPFVLRTPHLCAMQQDNSEDLGQVKSIMHDIVDVNLSSLAARRVIIEVSDCIIKRGGRLAGAGIAGILQKMEDNTKGLIRRRKVVAMDGGLYENYPQYRVYMLEAMAELLGPRDSEDVVVEHTKDGSGIGAALLAAVNSKYAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IW12</accession>
    <name>I1IW12_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H(+)/Pi cotransporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100845257</name>
        <name evidence="12" type="ORF">BRADI_5g02750v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581010.1">
        <property type="nucleotide sequence ID" value="XM_003580962.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IW12"/>
    <dbReference type="EnsemblPlants" id="KQJ81735">
        <property type="protein sequence ID" value="KQJ81735"/>
        <property type="gene ID" value="BRADI_5g02750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845257"/>
    <dbReference type="Gramene" id="KQJ81735">
        <property type="protein sequence ID" value="KQJ81735"/>
        <property type="gene ID" value="BRADI_5g02750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845257"/>
    <dbReference type="eggNOG" id="KOG0252">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_14_1"/>
    <dbReference type="OMA" id="FSKHEGM"/>
    <dbReference type="OrthoDB" id="433512at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000175">
        <property type="entry name" value="Inorganic phosphate transporter 1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="22"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ81735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="511" mass="56241" checksum="F45808D26D1F1125" modified="2012-06-13" version="1">MAQLRVLQALDVARTQLYHFTAIVIAGMGFFTDAYDLFTISLVADLIGYKYYAGRHDKYLSVSAAISSIALCGTVPGQLLFGWLGDKMGRKRIYGVTLIIMVFCSLASGFSFSKRTGASVVTVLCFFRFWLGFSIGGDYPLSATIMSEYANKRTRGTFIAAVFAMQGFGNLAAGIVGMIASAAFLNSSPDNVDYVWRIVLMFGAIPALLTYYWRMKMPETARYTALVAKNAKMAAADMSTVLNMHIVPEEEAVDELPAREEDQYGLFSTEFLHRHGLHLLGTTVCWFVLDVSFYSLNIFMKDIFTIVDLVPDIAEADNNPLRRMINITGLHTLIALAGSLPGYFFAVVLVDRIGRVKIQLLGFAMMAALTLGLAIPYDSWREHKNKKGFAIMYALIFFFANFGPNTTTFILPAELFPARLRSTCHGISGAVGKIGAILGVFAFSFMEKAKFKTLLFVLVGCNLVGLVFTLLLPETNGKSLEEITGEMEESQPPDDEAAVAAPHHDIRTVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1IY57</accession>
    <name>I1IY57_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100828447</name>
        <name evidence="13" type="ORF">BRADI_5g11261v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239955.1">
        <property type="nucleotide sequence ID" value="XM_010241653.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IY57"/>
    <dbReference type="STRING" id="15368.I1IY57"/>
    <dbReference type="EnsemblPlants" id="KQJ82832">
        <property type="protein sequence ID" value="KQJ82832"/>
        <property type="gene ID" value="BRADI_5g11261v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828447"/>
    <dbReference type="Gramene" id="KQJ82832">
        <property type="protein sequence ID" value="KQJ82832"/>
        <property type="gene ID" value="BRADI_5g11261v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828447"/>
    <dbReference type="eggNOG" id="KOG0134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_0_0_1"/>
    <dbReference type="OMA" id="GLAYCHM"/>
    <dbReference type="OrthoDB" id="1663137at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009695">
        <property type="term" value="P:jasmonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000073">
        <property type="entry name" value="12-oxophytodienoate reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="12">
        <location>
            <begin position="9"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ82832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="369" mass="40809" checksum="72897048C6202151" modified="2012-06-13" version="1">MSNTGTAPLLTPYKMGRFDLSHRVVLAPLTRERSFGNVPQPHAILYYQQRATKGGLLIAEATGVSDTAQGYKDTPGIWTKEQVEAWKPIVDGVHAKGGIFFCQIWHVGRVSNHNFQPNGQAPISSTNKPLKPVVRANGIVVATISTPRRLETDEIPLVIDDFRIAARNAIEAGFDGVEIHGAHGYLIDQFLKDQVNDRTDKYGGSLENRCRFALEVVQAVVDEIGADKVGIRLSPFASYSDASDSNPEALGLYMAHALNEFGILYCHMVEPRMVNIGEKFETPHSLRPIRNAFKGTFIVAGGYGREDGNKAISDGYADLVAYGRLFLSNPDLPRRFEIDVPLNKYNRDTFYLSDPVVGYTDYPFLSSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZV6</accession>
    <name>I1IZV6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100828963</name>
        <name evidence="2" type="ORF">BRADI_5g16290v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581446.1">
        <property type="nucleotide sequence ID" value="XM_003581398.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZV6"/>
    <dbReference type="STRING" id="15368.I1IZV6"/>
    <dbReference type="MEROPS" id="I03.004"/>
    <dbReference type="EnsemblPlants" id="KQJ83688">
        <property type="protein sequence ID" value="KQJ83688"/>
        <property type="gene ID" value="BRADI_5g16290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828963"/>
    <dbReference type="Gramene" id="KQJ83688">
        <property type="protein sequence ID" value="KQJ83688"/>
        <property type="gene ID" value="BRADI_5g16290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828963"/>
    <dbReference type="eggNOG" id="ENOG502S0PJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090145_0_0_1"/>
    <dbReference type="OMA" id="QTTEWHV"/>
    <dbReference type="OrthoDB" id="1918435at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015066">
        <property type="term" value="F:alpha-amylase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011065">
        <property type="entry name" value="Kunitz_inhibitor_STI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002160">
        <property type="entry name" value="Prot_inh_Kunz-lg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107:SF5">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00197">
        <property type="entry name" value="Kunitz_legume"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00452">
        <property type="entry name" value="STI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50386">
        <property type="entry name" value="STI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00283">
        <property type="entry name" value="SOYBEAN_KUNITZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095645" evidence="1">
        <location>
            <begin position="30"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ83688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="236" mass="25120" checksum="7E132B7D3FF5A98D" modified="2012-06-13" version="1">MSSSPRARLLLISLVAAATLLVSCRGAAASPPPPPVYDTDGHELRADTSYHVLPLIRRGGPYGARRGGLTLAPLHGGQRCPLFVAQDASREHLGLPVRFAPHGKASASADPTTVVRVSTDVRVSFRAYTTCVQSTEWHVESGKNILGARRHVVTGPVVGGPSPISGRENAFRVERVERYGGVATDDEAREYKLMWCGDEAACQALGVFRDGEGGAWFLGAAAEQVHVVVFQKAPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZW9</accession>
    <name>I1IZW9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100831606</name>
        <name evidence="12" type="ORF">BRADI_5g16400v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580184.1">
        <property type="nucleotide sequence ID" value="XM_003580136.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZW9"/>
    <dbReference type="FunCoup" id="I1IZW9">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83708">
        <property type="protein sequence ID" value="KQJ83708"/>
        <property type="gene ID" value="BRADI_5g16400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831606"/>
    <dbReference type="Gramene" id="KQJ83708">
        <property type="protein sequence ID" value="KQJ83708"/>
        <property type="gene ID" value="BRADI_5g16400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831606"/>
    <dbReference type="eggNOG" id="KOG0061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="MAVTYWM"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IZW9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015245">
        <property type="term" value="F:fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080051">
        <property type="term" value="P:cutin transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000504">
        <property type="entry name" value="ABC transporter G family member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052215">
        <property type="entry name" value="Plant_ABCG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042:SF25">
        <property type="entry name" value="OS04G0528300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="492"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="532"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="560"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="648"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="70"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ83708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="715" mass="78868" checksum="AB52BE965F38BABE" modified="2012-06-13" version="1">MRGGGEAENTPRRTAAGQAMVELQANAATATSASGAMVVGLSPLSETLWRDSKSLPGAGAALIGEVSARLTWKDLSVTVALGPGKTQTVLDELTGFAEPGSLTALMGPSGSGKSTLLDALAGRLAANAFLSGNVLLNGRKAKLSFGAAAYVTQDDNLIGTLTVRETIGYSALLRLPDKMPREDKRALVEGTIVEMGLQDCADTVIGNWHLRGVSGGEKRRVSIALELLMRPRLLFLDEPTSGLDSSSAFFVTQTLRGLARDGRTVIASIHQPSSEVFELFDMLFLLSGGKTVYFGQASQACEFFAQVGFPCPPLRNPSDHFLRCVNSDFDKVKATLKGSMKARIERSDDPLDKITTSEAIRKLVSSYNRSQYYYAAREKVNDIARIKGTVLDSSGSQASFLMQACTLTRRSFINMSRDFGYYWLRLLIYLLVTVCIGTIYLDVGTKYTSILARAACAAFVFGFVTFMSIGGFPSFVEEMKVFQRERLNGHYGVAAFVIANTLSALPFLILICFLSGTVCYFMVRLHPGFSHYIFFVLNLYASVTVVESLMMAIASVIPNFLMGIIIGAGIQGIFMLVSGYFRLPYDIPKVFWRYPMQYISFHYWALQGQCQNDMDGLVFDNQYPDQPKIPGDFILKYIFQINVNRSKWIDLSVIFSMIFIYRLLFFIMIKVNEDVLPWVRGYIARKRLQKKVPAIGKTPSLRGYVVDPELGPNDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0C6</accession>
    <name>I1J0C6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aquaporin TIP5-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100822509</name>
        <name evidence="11" type="ORF">BRADI_5g17680v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC 1.A.8.10) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581502.1">
        <property type="nucleotide sequence ID" value="XM_003581454.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0C6"/>
    <dbReference type="SMR" id="I1J0C6"/>
    <dbReference type="STRING" id="15368.I1J0C6"/>
    <dbReference type="EnsemblPlants" id="KQJ83937">
        <property type="protein sequence ID" value="KQJ83937"/>
        <property type="gene ID" value="BRADI_5g17680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822509"/>
    <dbReference type="Gramene" id="KQJ83937">
        <property type="protein sequence ID" value="KQJ83937"/>
        <property type="gene ID" value="BRADI_5g17680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822509"/>
    <dbReference type="eggNOG" id="KOG0223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_3_4_1"/>
    <dbReference type="OMA" id="VPTAMFY"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006833">
        <property type="term" value="P:water transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000017">
        <property type="entry name" value="Probable aquaporin TIP5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF27">
        <property type="entry name" value="AQUAPORIN TIP5-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ83937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="26955" checksum="FF8F3D1E9E59EC96" modified="2012-06-13" version="1">MASNLRAHMKHCFSAASLRSYLAEFVSTFLFVFVAVGSAMSARMLTPDVTSDASSLVATAVAQSFGLFAAVFIAADVSGGHVNPAVTFALAIGGHIAVPSAIFYWSCQLLGSTFACLVLHYFSAGQAVPTTRIAVEMTGFGAAIVEGVMTFMLVYAVHVAADPRACGRSRGLATTAMGSLVVGLVAGACVLAAGSLTGASMNPARSFGPAVVSGDFKNQAVYWVGPMIGAAVAALVHQNLVFPSAPEPLPHEVRHGSVETVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1J1D8</accession>
    <name>I1J1D8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ubiquinol oxidase</fullName>
            <ecNumber evidence="18">1.10.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100830584</name>
        <name evidence="19" type="ORF">BRADI_5g20540v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the cyanide-resistant oxidation of ubiquinol and the reduction of molecular oxygen to water, but does not translocate protons and consequently is not linked to oxidative phosphorylation. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a ubiquinol + O2 = 2 a ubiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30255"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="EC" id="1.10.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="18">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the alternative oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.11" evidence="18"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580438.1">
        <property type="nucleotide sequence ID" value="XM_003580390.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J1D8"/>
    <dbReference type="FunCoup" id="I1J1D8">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ84389">
        <property type="protein sequence ID" value="KQJ84389"/>
        <property type="gene ID" value="BRADI_5g20540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830584"/>
    <dbReference type="Gramene" id="KQJ84389">
        <property type="protein sequence ID" value="KQJ84389"/>
        <property type="gene ID" value="BRADI_5g20540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830584"/>
    <dbReference type="eggNOG" id="ENOG502QSB5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041974_0_1_1"/>
    <dbReference type="OMA" id="AGVNTWI"/>
    <dbReference type="OrthoDB" id="16906at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009916">
        <property type="term" value="F:alternative oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106292">
        <property type="term" value="F:superoxide-generating NADPH oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102721">
        <property type="term" value="F:ubiquinol:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010230">
        <property type="term" value="P:alternative respiration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01053">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.140:FF:000001">
        <property type="entry name" value="Ubiquinol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.140">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002680">
        <property type="entry name" value="AOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038659">
        <property type="entry name" value="AOX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803:SF3">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01786">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 18" id="KW-0408">Iron</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQJ84389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="333" mass="37338" checksum="A10CF2022BEF33CC" modified="2012-06-13" version="1">MSSRMAGSVLLRHAGASRLFSATATSPAAAAARPFLAGGESVPGAWVRLMSTSAGSQAKQEAAKAAAPPKDKEGGEKKEVVVNSYWGIEQAKKLVREDGTEWKWSCFRPWETYTADTSIDLTKHHVPKTMLDKIAYWTVKSLRFPTDIFFQRRYGCRAMMLETVAAVPGMVGGMLLHLRSLRRFEQSGGWIRALLEEAENERMHLMTFMEVAQPRWYERALVIAVQGVFFNAYFFGYLISPKFAHRVVGYLEEEAVHSYTEFLKDLEAGKIDDVPAPSIAIDYWRLPANATLKDVVTVVRADEAHHRDVNHFASDVYYQGMELKATPAPIGYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EH66</accession>
    <name>A0A0Q3EH66_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100824418</name>
        <name evidence="19" type="ORF">BRADI_4g09312v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">Bd21</strain>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575633.1">
        <property type="nucleotide sequence ID" value="XM_003575585.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757931.1">
        <property type="nucleotide sequence ID" value="XM_014902445.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757932.1">
        <property type="nucleotide sequence ID" value="XM_014902446.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757933.1">
        <property type="nucleotide sequence ID" value="XM_014902447.2"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ87095">
        <property type="protein sequence ID" value="KQJ87095"/>
        <property type="gene ID" value="BRADI_4g09312v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824418"/>
    <dbReference type="Gramene" id="KQJ87095">
        <property type="protein sequence ID" value="KQJ87095"/>
        <property type="gene ID" value="BRADI_4g09312v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824418"/>
    <dbReference type="OrthoDB" id="2668416at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EH66">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF174">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0611">Plant defense</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="16">
        <location>
            <begin position="10"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="14">
        <location>
            <begin position="196"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="17">
        <location>
            <begin position="442"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="18">
        <location>
            <begin position="590"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="15">
        <location>
            <begin position="1194"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="15">
        <location>
            <begin position="1537"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="15">
        <location>
            <begin position="1864"/>
            <end position="2011"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQJ87095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2089" mass="236357" checksum="0B2F189FD06D6DA3" modified="2016-01-20" version="1">MAMVLDAFASYLQGMLTEMAADAIHLLLGVSVEIDKMSDKLGDLKNFLADADRRNITDKSVQGWVTELKRAMYDATDILDLCQLQVMERGESTVDAGCCNPFLFCIRNPFHAHDMGTRIKALNERLDSIGKRSATFKFIDLGSYEDRGRNMHASRHGNPNRETSGELDQLGVVGEKIEEDTRALVDKILQTREGVTNNIMVFAIVGVGGIGKTTLAQNVFNNQSIQSEFDKMIWLSINQNFDQTELLRTAITHAGGDHCGEKVLSVLQPILTKALKGKKFFLVMDDLWSHGSWEGVLQTPLVNAAASGSRVLITTRDEAVARGMTATWPHHYIDTLSPDDAWSLLKKQVVLSGERDEYDVNILKDIGLKIIQKCGGLPLAIKVMGGLLRQREMHRRDWEQVLDNSDWSTSKMPEDLNNAVYLSYQDMPPELKQCFLYYSLLPKSRGFNVDQVVAMWIGEGFIPGNSNHLEETGRNYYEGLLSRNLIQLDELDDFLFHQKFCSMHDVVRSFGQYMARDEALVAQDGEIDILDKLNSKKFLRLSIQLETDEVQSDDLDWVSLREQQQSMRTLISTVPIKMKPGDSLVTSSSLRTLYIDSEDVALVESLHQLKHLRYLNLISGKWNQANTGISALPEDIGKMIFLQYLGINNCKNLVSLPDSIVKLGHLRYVNLPEKVSMIPRGFRGLTNMRILCGFPALVDGDWCSLDELGPLSQLRFLRLVKLENASSAANASLSEKKHLTNLLLHCTPREKLNEDEQQRIEKVFNELCPPSSVENLDIYDYFGKKLPSWMMSTPRVPLNNLKSLLLCHLACCTQLPDGLCHLPCLQVLEVVRAPCIKRVGNEFLHTSQATAVPFPRLQKMLLNGMMELEEWEWEEKVQAMPRLEELLLWNCKLRRVPPGLASNARALRKLSIEDVKQLSYLENFPSAVELTVDECPDLERITNLPKLQKLTITRCRKLKSVNDVPALQRLVLQDDEMETVPGYLRDVNPRHFELRCSLTLLTSIAAGESGPEFAKFRHVDHVKARRLYTSDFYVLYTRGPCSFDTNVNPSFLSRGTLPIFEDAQRLESALKMPRRAFDYVCNLLKENSWQHANKYTFPDGRYLCLEDGVAVALIVLNSGETPATVGSSVALNESTVSQVTESFVVAMDTPGWPGTTEMEKIKYKFDKIRGLPNCCGVVHAARIPFGSQNSDREKKNEDLLMQVVVDSDMRFIDVHLGSPDNMKESSISHDSNLFKRCEKGTRLNGSKLNLSDGRQVGEYVIGDAGYPLLPWLLTPYHPENDLSDYQVEFNRRHSEAMAVVPRSALERLKDTWKFLHGRGWHPEDQYVLRNAIQTCCKLHTIVIEMECKAGTTSYGAGSKEVRQLADEDAAMARDILSQHLTSKPLESGGTLAIFEDAQRLESVLKMPRTTFDYVCSLLKESSLEIMNSYFFFDMRSFSLEERVAIALIMLNSGDPPATVGCYIGVNEATVSLVTRTFAGVMLERALEHLWWPGSTEIDKIKYKFDKIHGLPNCCGVVHTVHITSGSHNKDHEVNDDMLMQAVVDTDMRFINTWLTSPDSMKQSSILHDSELFTECEKGTGLNGSKLNLPDGLQVGEYIIGDAGYPLLPWLLTPYELENELSDSQVEFNRRHLEATTIVPRAVTRLKDTWKFLHGGAWRPQNHHWAIYACFVLHNIVIHMECKAGTTSYNSQHVRRLADEDAVVARDILSQHLTRKPLESGGTLTIFEDAQRLESVLKMPRRTFDYICSLLKESSLEIMNNYFFFDMRFFSLEERVAIALIMLNSGDPPATVGSFLGVNESTVSLVTKSFVDAMYERAKYHVYWPQSDEREKMKSKFAKIHGLPNCCGVLHTTHVSPASRSWDHQDKDSILFQGVVGPDMRFTSILVGLQAGNTNQLSFLHDSMLFKECEKGAWLNGNKLKVSSEEVGEYVIGDAGYPLLPWLLTPYELQNDLPDISSYQAEFNRRHSSARNITLKAMARFESTWKIMHSEWRPDNPREMTRAINVCCRLHNIVIGMEEGARMPSEQEVNYSDDQVRQLADEDAVRMRDILSQHLLTSISSQSEEEQQEIFASSGSGYRAKCEAREENWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ET93</accession>
    <name>A0A0Q3ET93_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g26082v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ET93"/>
    <dbReference type="EnsemblPlants" id="KQJ89500">
        <property type="protein sequence ID" value="KQJ89500"/>
        <property type="gene ID" value="BRADI_4g26082v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89500">
        <property type="protein sequence ID" value="KQJ89500"/>
        <property type="gene ID" value="BRADI_4g26082v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3ET93"/>
    <dbReference type="OrthoDB" id="610340at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47127">
        <property type="entry name" value="10A19I.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="9"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ89500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="296" mass="32726" checksum="CFDAB120DA0E6D1A" modified="2016-01-20" version="1">MEGGGVIVWTNSMSAMMLSNLANLVAIGVRTSSGFKRVHLNNCAKALNEHFKTGITHAQVSNHLKKWRKVWINVTKLRNLSGALWDEDTSTILLDPEHYANHIKVSWTDSSFFDANYLNTPIEHYNAMATIFGASMATGKFAKSENDPLNSVEVHDIEDEDTPNESATEVNMSPNQGDTSQTNQGESSHTKPERKKAKLDPKEEDPLLSTLKSGLERVATALEKSGKDPEAIPEGLWDDLASLLDGDNLPRFINKSHLAHYYACLVDKPRTAQAFVTLSTKNKLVLGGKVCHGHFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F145</accession>
    <name>A0A0Q3F145_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g03645v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F145"/>
    <dbReference type="EnsemblPlants" id="KQJ93287">
        <property type="protein sequence ID" value="KQJ93287"/>
        <property type="gene ID" value="BRADI_3g03645v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93287">
        <property type="protein sequence ID" value="KQJ93287"/>
        <property type="gene ID" value="BRADI_3g03645v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3F145"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ93287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="110" mass="12510" checksum="6FC1995CFC69979E" modified="2016-01-20" version="1">MLCVLRREMRVVQRPGRRVRADVRQVPSVGVRELQAAGRPDLRLPVLRALLRQLHFHRLLSSSSAACAPGLIHLDAWSLFPSLVTVTCKQINFVFVHYFPIHIAQVFTCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FW82</accession>
    <name>A0A0Q3FW82_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g01535v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FW82"/>
    <dbReference type="EnsemblPlants" id="KQK02453">
        <property type="protein sequence ID" value="KQK02453"/>
        <property type="gene ID" value="BRADI_2g01535v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02453">
        <property type="protein sequence ID" value="KQK02453"/>
        <property type="gene ID" value="BRADI_2g01535v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FW82"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK02453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="42" mass="4731" checksum="32BEBFF400111781" modified="2016-01-20" version="1">MYPQEGYCSSNQSTPALIQSRMGNHPSTTWQKQMKEVTEAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FZU3</accession>
    <name>A0A0Q3FZU3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g10550v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FZU3"/>
    <dbReference type="EnsemblPlants" id="KQK03901">
        <property type="protein sequence ID" value="KQK03901"/>
        <property type="gene ID" value="BRADI_2g10550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03901">
        <property type="protein sequence ID" value="KQK03901"/>
        <property type="gene ID" value="BRADI_2g10550v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FZU3"/>
    <dbReference type="OrthoDB" id="686404at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994:SF41">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN LEA-2 SUBGROUP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994">
        <property type="entry name" value="OS04G0515000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK03901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="158" mass="17248" checksum="DE91792E078A2CDF" modified="2016-01-20" version="1">MNLYVAIDSVSGLDFLPPPPANIAIAAARRRPHRPDDQPGVQPHDARTYAEVSYRCVPLVASRALPAYLCIGVRKSADWRDAARGVGVRLPGFLLDGLAEDARSGVEAFDVTLKRVGADEEPGILAMCGVRRVGDAVEKCDTESYRFKKCFRSTKHPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GB28</accession>
    <name>A0A0Q3GB28_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100835524</name>
        <name evidence="5" type="ORF">BRADI_2g37350v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol polyphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314207.1">
        <property type="nucleotide sequence ID" value="XM_024458439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GB28"/>
    <dbReference type="EnsemblPlants" id="KQK07733">
        <property type="protein sequence ID" value="KQK07733"/>
        <property type="gene ID" value="BRADI_2g37350v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835524"/>
    <dbReference type="Gramene" id="KQK07733">
        <property type="protein sequence ID" value="KQK07733"/>
        <property type="gene ID" value="BRADI_2g37350v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="62798at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045849">
        <property type="entry name" value="IP5P_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666:SF15">
        <property type="entry name" value="TYPE I INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666">
        <property type="entry name" value="TYPE IV INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="4">
        <location>
            <begin position="104"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="224"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK07733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="567" mass="62822" checksum="2DB2C98733B5B344" modified="2016-01-20" version="1">MRTESMRTDHNARIPKKSLSWPRRTTAAVKKWLNVKNTDQQLAPDFIDESFGKVGQQRRKSCSDRDGCSRPLNTRDVSGGWLVEENLGRPPPGYGSSSSWHPPNKLRVLVGTWNVGGRAPHQGLDISEWLLDHGQASSSPPHIYVLGFQEIVPLNAGNVLGAEDKGPASKWLGLIGQALNPSSTSERTQSFSNNYWPAATEAKTPGNGFQKAKVSFSDLLAMDDTVSEEPEEDEGNDSEPSTSNPDSSSEEEEMTREFTHGRRAGGAGHSGYYRLAASKQMVGIFLCVWVRADVMPRVTSLRVSCVGTGIMGYMGNKGSISISLTLQEGRSTSTSLCFVCTHLASGEKDGDEVRRNCDVAEILRRTRFPPRAWLSAPETILEHDKVVWLGDLNYRLSTSGGDMRGLLERNEWRALLEKDQLRAEQRAGRVLDGWEEGEIRFPPTYKYLADSDTYAMTMNSSSSSGSGKSSSSREKKKRTPAWCDRILWRGEGMEQAWYERRESRFSDHRPVASLFSLTLHAANCNNAPSCCSRSTAKGAAVEAEEMLAVLPPRRSRTSCHHHSSSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GC60</accession>
    <name>A0A0Q3GC60_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_2g39666v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GC60"/>
    <dbReference type="EnsemblPlants" id="KQK08109">
        <property type="protein sequence ID" value="KQK08109"/>
        <property type="gene ID" value="BRADI_2g39666v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08109">
        <property type="protein sequence ID" value="KQK08109"/>
        <property type="gene ID" value="BRADI_2g39666v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GC60"/>
    <dbReference type="OrthoDB" id="690341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GC60">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF901">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="3">
        <location>
            <begin position="38"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="4">
        <location>
            <begin position="268"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="359"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK08109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="742" mass="83560" checksum="52B5B0D250D56CDF" modified="2016-01-20" version="1">MKYKIDESSRTRNEKVDPRLRAVYKDASELVRIDGPTDELVKWLRDKEGEVAHHPKLVSVVGYGGLGKTTLARQVYNKLGANFECRAFVSISRSPDMMKILGSILSEVSNGKEHARSTHQQIIDQIRDILKDKRYFIIIDDVWDIETWKTLDCALVKNDCGSIQPLSVTDSKKLFFKRIFGCEEKCPPNLKIASEDILKRCGGLPLAINALSSLLAAGKTKEEWDRVRLSISFAQGKNCDIDAMNYILSFSYFDLPLDLRSCLLYLTMFPEDYVVERQRLVHRWIYEGCIHGKGGEDLIELGVAYFHELVNRSLIQPVNIGYDGKALACRVHDTILDFLIGKSIEENFSTLMEQLDLSHARSLAVFGNVGQMPSLVKSNALRVLDLAVCDGLKNHHIKDIGRLLQLRYLVFHWSGITELPTQIGDLVYLETLDVSQTILLELPKSATCLKRMAQLFVSASTRLPDCIGNMEKLQELGHLIDIFKQSVKFLEELGKLINLRKLSVHLDYDEPDKASYEKKEMLVSSLCKLDTCKLHTLSIVFSFGGKDAETLIEGHPCFIPALKSIREISLIGGQLCWITKWMLSLANLEKLTLWGQRTGQQDVEMVGSIPSLLEFTGSSFSEPVSISSNGGFQQLQVFGFSLCVREFMFEAGAMPNVRRLYVKIWPKDINSASGCRGGFDDIGIQHLSSLAELCVSIYCQGTRAADVEAVEVAFKSMAEANPNRPKLEMRRYQAEEMLKDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GGG3</accession>
    <name>A0A0Q3GGG3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g48345v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09445.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GGG3"/>
    <dbReference type="EnsemblPlants" id="KQK09445">
        <property type="protein sequence ID" value="KQK09445"/>
        <property type="gene ID" value="BRADI_2g48345v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09445">
        <property type="protein sequence ID" value="KQK09445"/>
        <property type="gene ID" value="BRADI_2g48345v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GGG3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK09445.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="43" mass="5214" checksum="FFE058FF4E6261AD" modified="2018-03-28" version="2">MTIHCTTRHVKNPTLGCLHGPSKSDSRTWMRWRFENRILVYRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GJJ3</accession>
    <name>A0A0Q3GJJ3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histidine kinase</fullName>
            <ecNumber evidence="4">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100824891</name>
        <name evidence="19" type="ORF">BRADI_2g59127v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">Bd21</strain>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Cytokinin receptor related to bacterial two-component regulators. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="4"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564955.1">
        <property type="nucleotide sequence ID" value="XM_003564907.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GJJ3"/>
    <dbReference type="STRING" id="15368.A0A0Q3GJJ3"/>
    <dbReference type="EnsemblPlants" id="KQK11271">
        <property type="protein sequence ID" value="KQK11271"/>
        <property type="gene ID" value="BRADI_2g59127v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824891"/>
    <dbReference type="Gramene" id="KQK11271">
        <property type="protein sequence ID" value="KQK11271"/>
        <property type="gene ID" value="BRADI_2g59127v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824891"/>
    <dbReference type="OrthoDB" id="10266508at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GJJ3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019955">
        <property type="term" value="F:cytokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000137">
        <property type="entry name" value="Histidine kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000015">
        <property type="entry name" value="Histidine kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.350:FF:000001">
        <property type="entry name" value="Histidine kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.350">
        <property type="entry name" value="CHASE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006189">
        <property type="entry name" value="CHASE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042240">
        <property type="entry name" value="CHASE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056839">
        <property type="entry name" value="Receiver_AHK4/CRE1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF73">
        <property type="entry name" value="HISTIDINE KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03924">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24896">
        <property type="entry name" value="Receiver_CRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01079">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50839">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="CHASE" evidence="18">
        <location>
            <begin position="149"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="16">
        <location>
            <begin position="441"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="17">
        <location>
            <begin position="728"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="17">
        <location>
            <begin position="876"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="14">
        <location>
            <position position="779"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="14">
        <location>
            <position position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQK11271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1019" mass="113239" checksum="8943800668404208" modified="2016-01-20" version="1">MTGARLARGGGGAAGTTTMEAEKGGGAGIGVGFLGMGLYRLRLLPLPPLPEKLSSAKTLRSHVYANYLGSRPVRERWWRWLLLLWVLGWTLASCWIFYYENSQAVEKRRESLASMCDERARMLQDQFNVSMNHLQALAILVSTFHHAQTPSAINQATFARYAERTAFERPLTSGVAYAVRVTHGERDQFERQQGWSIKKMYSSKKKSPGPGPGDAATAEIREPAEEYAPVIFAQDAYKHVLSFDMLSGDDDRENILRARESGKGVLTAPFKLLNNRLGVISTYTVYKSEFPAYARPQERIQAAIGYLGGIFDIEALVDKLLHQLAGKQSIMVNVYDTTNEKPISMYGSNDTGGGMYHNSTLNFGDPSRRHEMHCRFMQKPPLPWLAITSSLGTLVIALLTGYIFHATVHRIAKVEDDYQNMMELKKRAEAADIAKSQFLATVSHEIRTPMNGVLGMLQMLMDTDLDTTQQDFVRTAQASGKALVSLINEVLDQAKIESGKLELEAVPFDLRIVCDDILSLFCGKAQEKGLELAVYVSDQVPQTLIGDPGRMRQIITNLMSNSIKFTERGHIYLTVHVVEEVMGCLEVKTGTHYTNTLSGYPVANRRRSWESFRLFDMDLHSSEMPFTPVTPDTIRLIISVEDTGAGIPFEAQYRIFTPYMQVGPSIARIHGGTGIGLSISKCLVHLMKGEIGFVSKPHVGSTFSFTAVLTRAQSNGNLNKSSGFKGINALVVDHRPVRAKVAKYHLQRLGVQTELTTDVNQIIPKLNCGPLAAKLVLVDKETWLKESHSMPHLVSKLRNKDQADPPKFFLLENPTSSIKSSSHISSEHNLNVIKKPLRASMLQVSLRQALGGVDKVHCKNGVVGNSTLGSLLHKKQIIVVDDNAVNLKVAAGALKKYGAVVTCADSGKKAIAYLSPPHIFDACFMDIQMPEMDGFQATKEIRKMESKLNEKIESGDVPPECVNVRRWRTPILAMTADVIQATYEECLKCEMDGYVSKPFEGEQLYREVTRFFQNHDQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H3I5</accession>
    <name>A0A0Q3H3I5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC112272527</name>
        <name evidence="3" type="ORF">BRADI_4g14761v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024319390.1">
        <property type="nucleotide sequence ID" value="XM_024463622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H3I5"/>
    <dbReference type="STRING" id="15368.A0A0Q3H3I5"/>
    <dbReference type="EnsemblPlants" id="KQJ87994">
        <property type="protein sequence ID" value="KQJ87994"/>
        <property type="gene ID" value="BRADI_4g14761v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63366">
        <property type="protein sequence ID" value="PNT63366"/>
        <property type="gene ID" value="BRADI_4g14761v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63367">
        <property type="protein sequence ID" value="PNT63367"/>
        <property type="gene ID" value="BRADI_4g14761v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112272527"/>
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        <property type="gene ID" value="BRADI_4g14761v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT63366"/>
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        <property type="protein sequence ID" value="PNT63367"/>
        <property type="gene ID" value="BRADI_4g14761v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052398">
        <property type="entry name" value="Ubiquitin_hydrolase_53/54"/>
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        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22975">
        <property type="entry name" value="UBIQUITIN SPECIFIC PROTEINASE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ87994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="237" mass="26819" checksum="86CC48402D40E1DE" modified="2016-01-20" version="1">MASTSSCQESVIVFNKHGADKDLFNFSLIIQSLCNLRHFRDKFLTEPLVWAPSVDNPCMAQIFYEIFSSWEKNEHYLTNILLNYMKTLLCGLAACTSFYEKLQVRKFFASEIVATILIGSHMSETSSRFSFNKVTERQVVNPITCGDCICPTHKLFGIKFEAQMSCGCGKSSDGYLYTTLFHKLDAGSPQTTKIKSFADLPVLLDEQFCKENNCKDCEDLQAVELSLSNTAHFITMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H4E4</accession>
    <name>A0A0Q3H4E4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBZ4-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100836299</name>
        <name evidence="2" type="ORF">BRADI_5g13070v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579948.1">
        <property type="nucleotide sequence ID" value="XM_003579900.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H4E4"/>
    <dbReference type="STRING" id="15368.A0A0Q3H4E4"/>
    <dbReference type="EnsemblPlants" id="KQJ83106">
        <property type="protein sequence ID" value="KQJ83106"/>
        <property type="gene ID" value="BRADI_5g13070v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83107">
        <property type="protein sequence ID" value="KQJ83107"/>
        <property type="gene ID" value="BRADI_5g13070v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61274">
        <property type="protein sequence ID" value="PNT61274"/>
        <property type="gene ID" value="BRADI_5g13070v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61275">
        <property type="protein sequence ID" value="PNT61275"/>
        <property type="gene ID" value="BRADI_5g13070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836299"/>
    <dbReference type="Gramene" id="KQJ83106">
        <property type="protein sequence ID" value="KQJ83106"/>
        <property type="gene ID" value="BRADI_5g13070v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83107">
        <property type="protein sequence ID" value="KQJ83107"/>
        <property type="gene ID" value="BRADI_5g13070v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61274">
        <property type="protein sequence ID" value="PNT61274"/>
        <property type="gene ID" value="BRADI_5g13070v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61275">
        <property type="protein sequence ID" value="PNT61275"/>
        <property type="gene ID" value="BRADI_5g13070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836299"/>
    <dbReference type="OrthoDB" id="1929441at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H4E4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35767">
        <property type="entry name" value="HAPLESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35767:SF1">
        <property type="entry name" value="HAPLESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="595"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="778"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="784"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ83107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1232" mass="135907" checksum="FD3E72F13D8C7AF3" modified="2016-01-20" version="1">MLPSGHPSGPSYSSKSVAGAAVGADPVTFAADTPSLECQDHADLIPPCPKFSIRDYVLDSRSKGIKRSWPFHPESLELCLNCDVKDVLPPFEPPGLIRSRFSYTCIHFEQSAVCSEVDASAGLVKIKDASSSHVNTTAINFQSCRSVDESLGLSQYTPKEEGKTATEEGRNTNEPSHTTEFIQADQQDKFCTKAMRRIGVAVPSCRLKNLGSSHETSEKKCNLIVRSGSVTNIRQRKDVSSNSSSVSDPKAWKTCPVCKVFSSTSNTTLNAHIDQCLYAESNTELVVETVIVKPKVKQRKKQLMVEIYKTALPYTLEDLDRRNGTNWAIEWSLRTSNKEVCTKKRSPEVVPFEARDDERDRDVYVDSNGIKIRILSKCSDAPLVFRDKLSLKKVAKHETGKSISMTKTFLESKTFRNEKFKVHGKKFNRLNHFNSQDQAYPDGDMHDYTSEEEPAMHTRKPAESTSCGGLETIKQRVCSKRVDIAKNFSRKLDSKASDSTQAFSPKSPEEMAITLEASDDDRGNGSSKLLGSIPRWSSNNLSSSSVMPKVPRSAAALAKRKIKEIGRREAYTSDNYDKARNSTLLKSSGVHRLSISNTRPSYDPNRVASTSKVLRKHRSVPRARKREFSPFISGLVHGFGQQHELDHRHVNKKFGVTNNDTPKKVVKRSQEDTADNGVSYGIDVSALGQGNDQYDVAQQTTDTNMDFEGEEPATRVQYASASRNTHEDCCSVISSGSLSPENSNTDDDVLAKGNVAREDPCSTEQSTHHTHVSNIVANNEMGERQTNAASTKESSASFTNNRDMAITTPQDNSSITSNRDDFNQDHGFLAFDRDLSDSPISIASTMPPPIALKDSSIKESEPGPSTVDVRTIQENMSGSSNKETKSMPLAREGEHLPNEKHYCCSCQASISRESQAHHETSTARSLTFTKEQVPQLDIGLRASSSFRSYQRTGRKANPCLDLHDQPSASKVSTESTMCFPSDTANYIRPSLQTPTPSPPSPMLRLMGKNLMVMNSQESGHPQAPSSEYAPRGNYMAPVGFMSPNYQHSDSAFINRTPAITSHQIPIPSVQAGNFVGPPFHGGSMVQFNHQSLQKPYRNVAPVMHHPAYMMNEVMMMKDSPVFRSGPQVGLLLPTGTYPASISVPNTTAPRPFYCLPSPIQILPKGSVDGSMPVFTNINPVIGVSTVSQSKQVRYRQSLHLTSSLVQSPEGYINPPVYYLQNLPQQLSNPNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H7Q9</accession>
    <name>A0A0Q3H7Q9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g43921v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H7Q9"/>
    <dbReference type="STRING" id="15368.A0A0Q3H7Q9"/>
    <dbReference type="EnsemblPlants" id="KQK18661">
        <property type="protein sequence ID" value="KQK18661"/>
        <property type="gene ID" value="BRADI_1g43921v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18661">
        <property type="protein sequence ID" value="KQK18661"/>
        <property type="gene ID" value="BRADI_1g43921v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H7Q9"/>
    <dbReference type="OrthoDB" id="691737at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H7Q9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF307">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="333"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK18661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="468" mass="54920" checksum="94B30609446F1E78" modified="2016-01-20" version="1">MDRIPFTKRSLKTLCGKISREQLDNDAAKTLDMFSKMLEADPDFKYTVQVEDDSRIKNLMWTSGKSMDQYICFGDVIAFDTTYGTNLYDMPFGLFVAVNNHFQSIILGGVLMRDEKVESFKWVFAEFMRMIGEKDRHPNTILTDQARSMEVAIAEVLPNMKHHWCKWHVLKKAKESLRALYGKKSEFRTEFHRLVNLMCTEEEFEKCWAEMLEKYGLQKQPYLTQIYEVRRKWAKPYFREIFCAKMTSTQRSESANHVLKTYVPPGCPMHLFVKQYEKLRFDRDSKEIYQEKRTSLTLFKSGQYVPEEMEAGSKFIVHRASHVFTEKWIKSIYRVQVDEGEGLFKCECARFEHCVMPCCHQLKVLLYLDYMEIPKTLIMKRWTRDATDILPPHLRSYEREHGRPRAVTYRHSHLALAAFEVVRMGDSNTTAYEKAIEGMVNLKKSLEPLSVTHDGLGLADREHSTAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HA89</accession>
    <name>A0A0Q3HA89_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter C family member 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Multidrug resistance-associated protein 13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">OsMRP5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Protein LOW PHYTIC ACID 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100827867</name>
        <name evidence="20" type="ORF">BRADI_1g48697v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">Bd21</strain>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ABC transporter that may affect phytic acid transport and compartmentalization. May function directly or indirectly in removing phytic acid from the cytosol or in vesicle trafficking. Required for phytic acid accumulation in developing seeds. Phytic acid is the primary storage form of phosphorus in cereal grains and other plant seeds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564288.2">
        <property type="nucleotide sequence ID" value="XM_003564240.4"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK19509">
        <property type="protein sequence ID" value="KQK19509"/>
        <property type="gene ID" value="BRADI_1g48697v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827867"/>
    <dbReference type="Gramene" id="KQK19509">
        <property type="protein sequence ID" value="KQK19509"/>
        <property type="gene ID" value="BRADI_1g48697v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827867"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HA89">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000003">
        <property type="entry name" value="ABC transporter C family member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000923">
        <property type="entry name" value="ABC transporter C family member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000169">
        <property type="entry name" value="ABC transporter C family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000002">
        <property type="entry name" value="ABC transporter C family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056228">
        <property type="entry name" value="ABCC10-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF421">
        <property type="entry name" value="ABC TRANSMEMBRANE TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24358">
        <property type="entry name" value="ABCC10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="631"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1014"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1053"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1142"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="407"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="719"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="1027"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="1333"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
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    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KQK19509.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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    </evidence>
    <sequence length="1576" mass="174475" checksum="44E19DFD3CB6B1B9" modified="2016-01-20" version="1">MRGTARPPATATATALPAAPTRAVTTSRALSWHVTLLLYPPAASTDEKEAPRRPVTHGLASDCPFLPPPQPGCADNQARGSRWRGPEQDLIHPAQMGSLTSSWMMNLCGSTVCSNQHVISCALKEIFDSSTCMNHLVAIGIGALLILTLSLRLLVKIPKTRASAQQLVKLGSPLQLAAVIFSGCLGLVYLGLGLWMLGIFNEFTPVYLPHWWLVTLSQGFSLILSSFAFSIRPWFLGASFVRFWSVLVTMYAAFICCSSVVDIVAEKAITIKACLDVLSLPGALLILLYGIQHSHDEDGYEGIGNIVYKPLNTEADGEIIGSESEVTPFAKAGVFSKMSFWWLNHLMKMGYDKPLEDKDVPDLQTTDRAHNQYLMFLEKLNSKQSQSHAKPSIFWTIVSCHKRGIMVSGFFALLKVLTLSLGPLLLKAFINVSLGKGTFKYEGFVLAVTMFVCKCCESLAQRQWYFRTRRLGLQVRSFLSAAIYKKQQKLSNSAKLRHSSGEIMNYVTVDAYRIGEFPYWFHQTWTTSVQLCIALAILYNAVGAATVSSLLVIIITVLCNAPLAKLQHKFQSKLMEAQDVRLKAMSESLVHMKVLKLYAWEAHFKKVIEGLREAEYKWLSAFLLRRAYNSLLFWSSPVLVSAATFLTCFILEIPLDASNVFTTVATLRLVQDPVRSIPDVIAVVIQAKVAFTRISKFLDAPELNGQVRKKYCVGMDYPIAMSSCGFSWDENSSRPTLKNINLVVKAGEKVAICGEVGSGKSTLLAAVLGEVPKTGGTIQVCGKIAYVSQNAWIQTGTLQDNILFGSLMDKQIYQETLVRCSLVKDLELLPFGDQTQIGERGVNLSGGQKQRVQLARALYQNADIYLLDDPFSAVDAHTATSLFNDYVMGVLSDKTVILVTHQVDFLPVFDSILLMSDGEVIRSAPYQDLLVDCQEFIDLVNAHRDTAGVSDLNHMGPDRALEIPTKETDLVHGNKYIESVKPSPVDQLIKKEERESGDSGLKPYMLYLRQNKGFLYASLSIISHIVFLAGQISQNSWMAANVQNPRVSTLKLISVYVVIGVCTVFFVLSRSLFVVVLGVQTSRSLFSQLLNSLFRAPMSFFDCTPLGRVLSRVSSDLSIVDLDVPFGFMFCLSASLNAYSNLGVLAVVTWEVLFVSLPMIVLAIQLQRYYLASAKELMRINGTTKSALANHLGESISGAITIRAFEEEDRFLAKNLELVDKNAGPYFYNFAATEWLIQRLETMSALVLSSSAFIMAILPQGTFSPGFVGMALSYGLSLNNSFVNSIQKQCNLANQIISVERVNQYMDIQSEAAEVIEENRPGPDWPQVGSVELRDLKIRYRRDAPLVLHGISCKFQGRDKIGIVGRTGSGKTTLIGALFRLVEPVGGKIIIDSVDITTIGLDDLRSRLGIIPQDPTLFQGTVRYNLDPLGQFSDQQIREVLDKCQLLEAVQEKEHGLDSLVAEDGSNWSMGQRQLFCLGRALLRRCRILVLDEATASIDNATDAVLQKTIRTEFKYCTVITVAHRIPTVMDCDMVLAMSDGRVVEYDKPTKLMETEGSLFCDLVKEYWSYTSNGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HGZ6</accession>
    <name>A0A0Q3HGZ6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g00471v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92754">
        <property type="protein sequence ID" value="KQJ92754"/>
        <property type="gene ID" value="BRADI_3g00471v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92754">
        <property type="protein sequence ID" value="KQJ92754"/>
        <property type="gene ID" value="BRADI_3g00471v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33115:SF58">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ92754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="76" mass="8415" checksum="6D98FCD378547C3B" modified="2016-01-20" version="1">MADRKKLLADGEWKEVDYINTYGVFMVYLSMAIKGSGFLVLTWTTVVLLGGFVSKLSKKDFWSLAFIALSQTGGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HJ89</accession>
    <name>A0A0Q3HJ89_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100837607</name>
        <name evidence="6" type="ORF">BRADI_1g70250v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Leucine-rich repeat protein that likely mediates protein interactions, possibly in the context of signal transduction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SHOC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228985.2">
        <property type="nucleotide sequence ID" value="XM_010230683.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HJ89"/>
    <dbReference type="STRING" id="15368.A0A0Q3HJ89"/>
    <dbReference type="EnsemblPlants" id="KQK22944">
        <property type="protein sequence ID" value="KQK22944"/>
        <property type="gene ID" value="BRADI_1g70250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837607"/>
    <dbReference type="Gramene" id="KQK22944">
        <property type="protein sequence ID" value="KQK22944"/>
        <property type="gene ID" value="BRADI_1g70250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837607"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HJ89">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="85"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK22944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="262" mass="29171" checksum="36462A601C28618E" modified="2016-01-20" version="1">MGCCTSRSSDSPASRVTRWRSTGIVALRDARLKVVPNEVLQVGNSLRTLDLTNNKIVEIPQEIGKIVNMQRLVLAGNLIEIIPADIGYLRNLKILTLDRNRITILPEELGSLSNLQQLTISQNSLLCLPKSVGDLRNMLLLNVSDNKLNAFPESIGGCNSLEELQANGNSIEDLPSSICNLVCLKSISLNRNKIRQLPQGLLKDCKALQNISLHDNPISMDQFQQMDGFEEFEARRRKKFDKQIDSNVMMSSTALDEGVDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HWD8</accession>
    <name>A0A0Q3HWD8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100842568</name>
        <name evidence="9" type="ORF">BRADI_3g33170v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234992.1">
        <property type="nucleotide sequence ID" value="XM_010236690.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HWD8"/>
    <dbReference type="STRING" id="15368.A0A0Q3HWD8"/>
    <dbReference type="EnsemblPlants" id="KQJ97770">
        <property type="protein sequence ID" value="KQJ97770"/>
        <property type="gene ID" value="BRADI_3g33170v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67867">
        <property type="protein sequence ID" value="PNT67867"/>
        <property type="gene ID" value="BRADI_3g33170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842568"/>
    <dbReference type="Gramene" id="KQJ97770">
        <property type="protein sequence ID" value="KQJ97770"/>
        <property type="gene ID" value="BRADI_3g33170v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67867">
        <property type="protein sequence ID" value="PNT67867"/>
        <property type="gene ID" value="BRADI_3g33170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842568"/>
    <dbReference type="OrthoDB" id="71302at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HWD8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11445">
        <property type="entry name" value="bHLH_AtPIF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000059">
        <property type="entry name" value="transcription factor UNE10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031066">
        <property type="entry name" value="bHLH_ALC-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047265">
        <property type="entry name" value="PIF1-like_bHLH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45855:SF23">
        <property type="entry name" value="TRANSCRIPTION FACTOR MEE8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45855">
        <property type="entry name" value="TRANSCRIPTION FACTOR PIF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="260"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ97770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="452" mass="48710" checksum="95436F0B9D7EAD2E" modified="2016-01-20" version="1">MNNQCVPSWDLEDTAGLNQRSASGPAHQPRGLAAPVVAVPMPEPDQYDEVAELTWEKGNIFWQGLLSRPAPIKYPPPSAAAPMHASPGDRREQTLEAVVGEAAARSSSHSHHLAPNWLAVGVDQVAAGVVPCAAGDADEAEEDARVRNDNKRARRLQACAASQGSAAAPGAARDSPTLLTMEPCGFTATTTNNSTSLDLDHGSPEETTENTSFGGGAASDSRYFSRRSQRDGLCDEAETVVIKEEPPMRMRSSISTKRSRAAAIHNESERKRRDRINQKMQTLQKLVPNSSKTDKASMLDEVIEHLKQLQAQVQMMSRMGSMMMPMGMAMAPPLQMSVMANMAQMAQMAQMGLGMMNMAAQPAAYPGLASPMNIMQPPNPFVPIQPWDAAGDRQKQPAGIAAVPAYSAFLACQAAAQQQQQAQPNGMEAYNRMVAMYQKLSQQQSQPGNSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J0Y9</accession>
    <name>A0A0Q3J0Y9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(+) kinase</fullName>
            <ecNumber evidence="2">2.7.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100826438</name>
        <name evidence="10" type="ORF">BRADI_2g61240v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="2"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567496.2">
        <property type="nucleotide sequence ID" value="XM_003567448.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J0Y9"/>
    <dbReference type="FunCoup" id="A0A0Q3J0Y9">
        <property type="interactions" value="2208"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3J0Y9"/>
    <dbReference type="EnsemblPlants" id="KQK11619">
        <property type="protein sequence ID" value="KQK11619"/>
        <property type="gene ID" value="BRADI_2g61240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826438"/>
    <dbReference type="Gramene" id="KQK11619">
        <property type="protein sequence ID" value="KQK11619"/>
        <property type="gene ID" value="BRADI_2g61240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826438"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3J0Y9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000006">
        <property type="entry name" value="probable NAD kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000018">
        <property type="entry name" value="Probable NAD kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK11619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="573" mass="63140" checksum="2A39D95F22C5A31D" modified="2016-01-20" version="1">MDRAAMPAQEELPLPSPEAIQFAQPADSRFRTLNPGPIPIPAAPSSRSLVESVSDQRVNLDVISSYQSENGSLSTVSSTVSSEESEKAAYELLAHTPIKSTDAHLVEFSEAMRTVAKALRRVAEGKAAAQAEAAEWKRKYELETAQKQQTKIKDCGSSTGNNLGKLASQRTLETPASDQTACCGNHGICSHEVLQDEVPGSNPRSSHKMVGRKASFRLSWGCNGDKNGQHKHDFVSFEKGDITTAERSNKQILLRWESRPQTVLFITKPNSNPVRVLCAEMVRWLKEHKNINVFVEPWVSKDLLTEDSSHNLVQTWDNDEERKVLHKKVDLIVTLGGDGTVLWAASLFKGPVPPVVAFSMGSLGFMTRFPSQQYRDCLDNVLNGPFSITLRNRLQCRVIRDAAKDELETEEPILALNEVTIDRGISSYLTYLECYCDSSFVTCVQGDGLIISTTSGSTAYSLAAGGSMVHPQVPGILFTPICPHSLSFRPLILPEYVTLRVQVPYNSRGHAWASFDGKDRKQLAPGDALICSISPWPVPTACLVDSTTDFLHSIHEGLHWNLRKTQSLDGPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JK51</accession>
    <name>A0A0Q3JK51_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100838803</name>
        <name evidence="8" type="ORF">BRADI_3g38500v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235228.1">
        <property type="nucleotide sequence ID" value="XM_010236926.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JK51"/>
    <dbReference type="STRING" id="15368.A0A0Q3JK51"/>
    <dbReference type="EnsemblPlants" id="KQJ98696">
        <property type="protein sequence ID" value="KQJ98696"/>
        <property type="gene ID" value="BRADI_3g38500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838803"/>
    <dbReference type="Gramene" id="KQJ98696">
        <property type="protein sequence ID" value="KQJ98696"/>
        <property type="gene ID" value="BRADI_3g38500v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838803"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JK51">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00257">
        <property type="entry name" value="beta-trefoil_FSCN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057232">
        <property type="entry name" value="DUF7910"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010431">
        <property type="entry name" value="Fascin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10551">
        <property type="entry name" value="FASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10551:SF36">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25490">
        <property type="entry name" value="DUF7910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129374" evidence="5">
        <location>
            <begin position="23"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7910" evidence="7">
        <location>
            <begin position="56"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="6">
        <location>
            <begin position="227"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ98696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="523" mass="58230" checksum="364E2B04566A7AC6" modified="2016-01-20" version="1">MRLPCTGVLVFCSLCVFQLSHSSSDDSFTKVRAVNLGGWLVVEGWIKPSLFDGIPNGDMLDGTQVQLKSVALQKYVSAYSGGGSNVTVDRAATSSWETFKLWRVSDSEFQFRCFNGQFLTASNGDTISATADSPGDPETFYIERNNTLLHIKLLNGNYLQVTDNNQLASNYHSQPGWGDDMATFEMTIVANNLHGDYQLANGYGPAQAKVVLTEHRKSFVTGKDFYFLSRNSINAVRIPVGWWIAYDPDPPAPFIGGSLDTLDRAFYWAQVYGLKCIIDLHAAPGSQNGMEHSASRDGSVDWPSAANIKKTLEVINFLAQRYADNPSLLGIELLNEPSADGVPLGTLVSYYKAGYKIVRSYSETAYVIFCQRIGNADPVELYQADLGPTNTVVDLHYYNLFDPYFEKLNATENIRFIYKDRLPQVQALSGANGPLVFIGEWVNEWNVANASQAQYQLFGKAQLEVFGEASFGWSYWTVRCNSVHWDYEWNIRNRYLAGGSPLESPNYMMLVAGCVLYILSIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3L4A4</accession>
    <name>A0A0Q3L4A4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100835151</name>
        <name evidence="4" type="ORF">BRADI_4g11137v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577273.2">
        <property type="nucleotide sequence ID" value="XM_003577225.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3L4A4"/>
    <dbReference type="FunCoup" id="A0A0Q3L4A4">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ87445">
        <property type="protein sequence ID" value="KQJ87445"/>
        <property type="gene ID" value="BRADI_4g11137v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835151"/>
    <dbReference type="Gramene" id="KQJ87445">
        <property type="protein sequence ID" value="KQJ87445"/>
        <property type="gene ID" value="BRADI_4g11137v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835151"/>
    <dbReference type="OrthoDB" id="412814at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3L4A4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032870">
        <property type="entry name" value="ALKBH7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21052:SF0">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21052">
        <property type="entry name" value="SPERMATOGENESIS ASSOCIATED 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="159"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ87445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="276" mass="30919" checksum="E7AF2DEAF5E16279" modified="2016-01-20" version="1">MVVDRNRVQGALHQFMAAAAASYDDDELRGRAALREAFGDSSESESDAPREEDPSPVGRGRWRWEAVAGVRGLWLCAAFLSADEQSRLLAAIQREGWFIDAHNQAMRFGDLPPWAVELSVLVREAICVGDVNVDFGPDSSEENEDSCPLPSDLLWREPLFDQLIANRYKPGEGICAHVDLMRFDDGIAIVSLESACVMHFSQESTAYDMLKNDESEFTNVPVYLSPGSLVVMSGDARYHWKHEINRKPGAQIWNGQELEQHRRTSVTLRKLRASPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LH73</accession>
    <name>A0A0Q3LH73_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106866788</name>
        <name evidence="3" type="ORF">BRADI_4g41021v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758075.1">
        <property type="nucleotide sequence ID" value="XM_014902589.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LH73"/>
    <dbReference type="EnsemblPlants" id="KQJ91989">
        <property type="protein sequence ID" value="KQJ91989"/>
        <property type="gene ID" value="BRADI_4g41021v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106866788"/>
    <dbReference type="Gramene" id="KQJ91989">
        <property type="protein sequence ID" value="KQJ91989"/>
        <property type="gene ID" value="BRADI_4g41021v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:106866788"/>
    <dbReference type="OrthoDB" id="784693at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34964">
        <property type="entry name" value="MEMBRANE LIPOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34964:SF12">
        <property type="entry name" value="OS12G0182000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ91989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="153" mass="16065" checksum="F55FF602ADD93AAE" modified="2016-01-20" version="1">MGRPDEPPAGISALCVWAVAALLLAAALAGGGCLALSLALPPADAPPWLPAAGLALVALPWAFWIATCLYRCCCSWSPSAPAGVVERQPSRSVVPLPSSANLKSALSRKNHEKRPPDEAPRRVRFGDSVVLGEEKKEKEDDDETPLASSMEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LK85</accession>
    <name>A0A0Q3LK85_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100826356</name>
        <name evidence="9" type="ORF">BRADI_3g01270v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in nitrate transport, but does not seem to be able to mediate transport by its own. Acts as a dual component transporter with NAR2.1. Imports nitrate with high affinity when expressed with NAR2.1 in a heterologous system (Xenopus oocytes).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572550.1">
        <property type="nucleotide sequence ID" value="XM_003572502.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LK85"/>
    <dbReference type="FunCoup" id="A0A0Q3LK85">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3LK85"/>
    <dbReference type="EnsemblPlants" id="KQJ92869">
        <property type="protein sequence ID" value="KQJ92869"/>
        <property type="gene ID" value="BRADI_3g01270v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826356"/>
    <dbReference type="Gramene" id="KQJ92869">
        <property type="protein sequence ID" value="KQJ92869"/>
        <property type="gene ID" value="BRADI_3g01270v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826356"/>
    <dbReference type="OrthoDB" id="605532at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LK85">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071249">
        <property type="term" value="P:cellular response to nitrate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015706">
        <property type="term" value="P:nitrate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17341">
        <property type="entry name" value="MFS_NRT2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000048">
        <property type="entry name" value="High affinity nitrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000053">
        <property type="entry name" value="Nitrate transporter 2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="HIGH-AFFINITY NITRATE TRANSPORTER 2.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ92869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="54692" checksum="261DF7C8A6C4529D" modified="2016-01-20" version="1">MEVETGDMAAKSKFSLPVDSEHKAESIRLFSFANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLAKADIGNAGVASVSGSIFSRLAMGAVCDLLGPRYGCAFLVMLAAPTVFCMSLIDDAAGYITVRFLIGFSLATFVSCQYWMSTMFNSKIIGTVNGLAAGWGNMGGGATQLIMPLVFHAIQKCGSTPFVAWRIAYFVPGLMHVVMGLLVLTMGQDLPDGNLRSLQKKGEMNKDKFSKVLWGAVTNYRTWIFVLLYGYCMGVELTTDNVIAEYYYDHFHLDLRAAGTIAACFGMANLVARPMGGYLSDLGARYFGMRARLWNIWILQTAGGAFCLWLGRASALPASVTAMVLFSICAQAACGAVFGVAPFVSRRSLGIISGMTGAGGNVGAGLTQLLFFTSSSYSTGRGLEYMGIMIMCCTLPVALVHFPQWGSMLFPANAGATEEEYYAAEWSEEEKSKGLHLPGQKFAENCRSERGRRNVILATSATPPNNTPQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QPV5</accession>
    <name>A0A0Q3QPV5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RWP-RK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100844863</name>
        <name evidence="8" type="ORF">BRADI_2g08177v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <scope>IDENTIFICATION</scope>
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            <end position="20"/>
        </location>
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        </location>
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        </location>
    </feature>
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        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK16906.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="746" mass="82326" checksum="45707EB3E19DC0F3" modified="2018-03-28" version="2">MEKAPRAILFLLLIVGFGVAQNTTSKGDEFHVGVILDLGTLAGKVARTSISLAVEDFYSVHRNHRTKLVLHIRDSMGSDVQAASAAIELLENYKVQAIVGPQKSSEVLFISNIGNITQVPIVSFTATSPSLTSDSMPYFVRATLNDSAQVNSIALLIKAYGWREVVPVYEDTDYGRGILPSLIEALQEIDARVPYRSVTPSSATSEIITQELYKLKAMQTRVFIVHMSPTMTSLFFTKAKEAGMMNKGFVWITTDGVANIIDSLDPSVIEAMNGVLGVRYYVPKSQELDSFTIRWNRMYQRDNPDESPFNKLSIVGLWGYDMIRALAQAAEKAGISSVTNKQPQSIKNSTCLDSMVISTNGPDLLTAIVQNKFRGISGDFDLTNRQLTVSVFQIINVVGRGWREIGFWTVNSGLSRQFNQTGLKITGPALMIDLNPVIWPGESTEIPRGWEIPTIGKKLRVGVRTSIYPEFIETFRDHATGETRASGLSVEIFEEAVKRLPFALTYEYLAFDTADPRSKGSYDDFVNQVYLQKYDIAVADTTIRCNRSLFVDFTVPYTESGVLPKNSPLRAEISRAILNITESAKIIQIEKKWVDSNSHQNDDKTDGSDAITFESFGGLFLLTGIVTACSLFVAVLMNGYKKYQQNGGSKGDNQNECGHEKKGEDGDSQGEQRDPNINDMEKQATLPVSHSLNTNGDQLPDSKDNDICCRCTQNNKATSLTHIGSQIIHRGDKSTRSSSGSKLVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3SCC2</accession>
    <name>A0A0Q3SCC2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g68469v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3SCC2"/>
    <dbReference type="EnsemblPlants" id="KQK22626">
        <property type="protein sequence ID" value="KQK22626"/>
        <property type="gene ID" value="BRADI_1g68469v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22626">
        <property type="protein sequence ID" value="KQK22626"/>
        <property type="gene ID" value="BRADI_1g68469v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3SCC2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK22626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="84" mass="9221" checksum="09D208B6C1FA6735" modified="2016-01-20" version="1">MACLFHFQLVPHGVARWRKGKGDFAACFSPFFFHPSFSPARFLPLPTFAALCVFGSPVGLSRSGSLIGRLREAGTQFSEELKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CF67</accession>
    <name>A0A2K2CF67_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g03159v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CF67"/>
    <dbReference type="EnsemblPlants" id="PNT60672">
        <property type="protein sequence ID" value="PNT60672"/>
        <property type="gene ID" value="BRADI_5g03159v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60672">
        <property type="protein sequence ID" value="PNT60672"/>
        <property type="gene ID" value="BRADI_5g03159v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CF67"/>
    <dbReference type="OrthoDB" id="649363at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF17">
        <property type="entry name" value="OS01G0114800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="139"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT60672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="326" mass="36933" checksum="1C87DFA52108C584" modified="2018-03-28" version="1">MDVLNSLFRKAEAVGLLGRLASHGLAQRASFFADDALCRAAISACVGNGRQVCFWTDQWLLGDSFDNLTPCLWKKLSPRAKRVSVAEALADDFWIRSIGTDLSLPEIEEFLSLWSSIHMVVLDVSDDLFRWGWCNSGSFAASSAYAAFFRSRELLPCAEEIWELRAPNKCKFFMWLAVRDRCWTTDRLSRRGLPHPASCPLCDQEPESISHLLLGCVAARQVWALILHRWGLPLWLPGIEENLQSWCSARISPERSRKDVNIGITLVCWCTWRHRNAVVFEGARPDFRTVLRLLDLEGVAWTRAGLLKDVRFSFASSVGALWSVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHF4</accession>
    <name>A0A2K2CHF4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g15328v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHF4"/>
    <dbReference type="EnsemblPlants" id="PNT61450">
        <property type="protein sequence ID" value="PNT61450"/>
        <property type="gene ID" value="BRADI_5g15328v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61450">
        <property type="protein sequence ID" value="PNT61450"/>
        <property type="gene ID" value="BRADI_5g15328v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CHF4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT61450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="183" mass="18876" checksum="7488F46B891EFA6A" modified="2018-03-28" version="1">MSPPSVPRAGVVLFQRVAVGPGPACRCPRPSQRTPAPPSGAPGGVLLGRQLRPCSGRLRPPLARRRPLPSAPSPPSAQAAAAALFRRRPPPASSAPTASSSGPPRPSSSVPKLPFAPLDVTAVPRCAEVPFAALAVVVLATAPVGLQRAGRRRGLCTGRRHPPRAPAPSSQRPSPTPPNSRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHT5</accession>
    <name>A0A2K2CHT5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_5g17333v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
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                <person name="Barry K."/>
                <person name="Shu S."/>
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</entry>
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    <reference evidence="2 3" key="1">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="2">Bd21</strain>
        </source>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </dbReference>
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        </location>
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        </location>
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        </location>
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        <source>
            <dbReference type="EMBL" id="PNT61901.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT61901"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A2K2CKG0</accession>
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        </submittedName>
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        <name evidence="2" type="ORF">BRADI_4g04426v3</name>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CKG0"/>
    <dbReference type="EnsemblPlants" id="PNT62501">
        <property type="protein sequence ID" value="PNT62501"/>
        <property type="gene ID" value="BRADI_4g04426v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62501">
        <property type="protein sequence ID" value="PNT62501"/>
        <property type="gene ID" value="BRADI_4g04426v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CKG0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="61"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT62501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="235" mass="25170" checksum="026262657EBF6FDA" modified="2018-03-28" version="1">MWRHTGHAVRAVPSMSCTNWSRSLLSPAATAPAGSAARPPPCQVAPVSIFFPWPSSLFSSPSSSSPHLPSFSQAEPGEDDEREDVRRHPRGTGPPAKRHCRSVRAPPATRAAANQLVRRSLSRRAHAMPPALFADPMRCSTSSALSRRSPTLALSPSPATSRSRRHRTSPLETPPPWPPSASIHASSSRPASEPDPGASCPPLRTRSVPVASLLTHASSDSLRFLLGLRWQRGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CLX6</accession>
    <name>A0A2K2CLX6_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g10457v3</name>
    </gene>
    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CLX6"/>
    <dbReference type="EnsemblPlants" id="PNT63025">
        <property type="protein sequence ID" value="PNT63025"/>
        <property type="gene ID" value="BRADI_4g10457v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63025">
        <property type="protein sequence ID" value="PNT63025"/>
        <property type="gene ID" value="BRADI_4g10457v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CLX6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="203" mass="21649" checksum="728931A9D5FF7AB4" modified="2018-03-28" version="1" fragment="single">LARRRALESTHVEHRGEDVGPDEVVGEATRGAEAAGGQGGGAVEKLTGDPHVRSTSATRWPRPSTVSCPDPTPSLPRLLPSSSFCSRMAAITSPSRPSGCTAPPQRPRPYPQASSPTPSLFLLHPCSFPGFLPHRELLSLILPRILSVFSSDEPRPAAPACPRPRIRANMVSCSASFQDLHRVDVDSLQGRRAAQRLSSPSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CNQ6</accession>
    <name>A0A2K2CNQ6_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g19710v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CNQ6"/>
    <dbReference type="EnsemblPlants" id="PNT63668">
        <property type="protein sequence ID" value="PNT63668"/>
        <property type="gene ID" value="BRADI_4g19710v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63668">
        <property type="protein sequence ID" value="PNT63668"/>
        <property type="gene ID" value="BRADI_4g19710v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CNQ6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="92" mass="10418" checksum="1345FFFDB7CC3AE4" modified="2018-03-28" version="1">MGSCSCHRTASTRLRPRLLIRVDESDSRDAAPFRVPGFYVEVARMHARLLEMDRGRGSEDMETGEGSPGWSSRGYLLHGRRRPRNHVAIPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CP67</accession>
    <name>A0A2K2CP67_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine carboxypeptidase-like 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g21637v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
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        <property type="protein sequence ID" value="PNT63837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CP67"/>
    <dbReference type="STRING" id="15368.A0A2K2CP67"/>
    <dbReference type="EnsemblPlants" id="PNT63837">
        <property type="protein sequence ID" value="PNT63837"/>
        <property type="gene ID" value="BRADI_4g21637v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63837">
        <property type="protein sequence ID" value="PNT63837"/>
        <property type="gene ID" value="BRADI_4g21637v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CP67"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF46">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033311531" description="Serine carboxypeptidase-like 19" evidence="2">
        <location>
            <begin position="33"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22024" checksum="0AC65198A3992915" modified="2018-03-28" version="1">MEMEVQASLPQRLLRFVLLLCFSSLCYRFVSSADSAPTPVSRLPGFDGDLHSTSRQGRYVSVEEENGAELFYYFIESEGDPRRDPVLLWLTGGDRCSVLSGLFFEIGPLKFVVEPYNEGSIPRLRYHPYSWAKFASILFVIRRSWFTEHQDYLANPFYVGGDSIAARIVPFLALKISEDIEAGRRPTINLKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CW45</accession>
    <name>A0A2K2CW45_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106866527</name>
        <name evidence="1" type="ORF">BRADI_3g09013v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756418.1">
        <property type="nucleotide sequence ID" value="XM_014900932.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CW45"/>
    <dbReference type="EnsemblPlants" id="PNT66250">
        <property type="protein sequence ID" value="PNT66250"/>
        <property type="gene ID" value="BRADI_3g09013v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106866527"/>
    <dbReference type="Gramene" id="PNT66250">
        <property type="protein sequence ID" value="PNT66250"/>
        <property type="gene ID" value="BRADI_3g09013v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="686565at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CW45">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34287:SF16">
        <property type="entry name" value="OS02G0234700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34287">
        <property type="entry name" value="OS06G0551500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT66250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="90" mass="9983" checksum="C96079392EBB47B9" modified="2018-03-28" version="1">MAASPSLLDLMSLSPIPPPDASSPVSAGATVQVVPRDVSEELLGKFMDTSEFGFDYDRSGLWSPLVPRPLAFAPAKPRRRSWRRKMFCCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CW75</accession>
    <name>A0A2K2CW75_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g09532v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CW75"/>
    <dbReference type="EnsemblPlants" id="PNT66277">
        <property type="protein sequence ID" value="PNT66277"/>
        <property type="gene ID" value="BRADI_3g09532v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66277">
        <property type="protein sequence ID" value="PNT66277"/>
        <property type="gene ID" value="BRADI_3g09532v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CW75"/>
    <dbReference type="OrthoDB" id="675134at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34303">
        <property type="entry name" value="OS01G0890400 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34303:SF8">
        <property type="entry name" value="OS09G0372600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="51197" checksum="1FCFBD517998BEED" modified="2018-03-28" version="1">MVAEGSFFIKDEEEEDPEERIPATPSEDGLPLHPLSDLIAAPVPLPPPALPEFIPAFSSYSDFEEEFLSGDSEYEPAPDAIPARGNGSPPPTRPDCVNVLMPHVPLENYANLAYAYIFPPAVSPAFFIRRALNPRPPCPTPQLRPFSQGSSLVIFNSTYDRELAMLNEPFRGYEHTVHLVRHDDTENRFLFDHLNLAGLAIDDFPLKHWFAKHIFASVVPFASPYKINPICITDVDYSAVLITVKACSINDVPHMLAFYCFFGIGTLANIDIVHSQALPPIHDNPFPPRSPRGSVRSDTASDDGDSDDSGGSGPSDPELAMPPPLPKEEPTPSDAAAGPAAAAAPGTERIVNLPGGALSPLVHASPLLSRPADVEVRLFLGFFKLHVTGADGERGLYRLPLERVGEMGMFVANLATCSMSPPRGCHERSLATEVDIICRDPAFTPPVSAVEHVQPVAAPPVDARFLHPPAPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CXM0</accession>
    <name>A0A2K2CXM0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_3g16758v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CXM0"/>
    <dbReference type="FunCoup" id="A0A2K2CXM0">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66766">
        <property type="protein sequence ID" value="PNT66766"/>
        <property type="gene ID" value="BRADI_3g16758v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66766">
        <property type="protein sequence ID" value="PNT66766"/>
        <property type="gene ID" value="BRADI_3g16758v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CXM0"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051420">
        <property type="entry name" value="Ser_Thr_Kinases_DiverseReg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005">
        <property type="entry name" value="LEUCINE RICH REPEAT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005:SF15">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="100"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT66766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="385" mass="42061" checksum="80EA809B7F2E9D05" modified="2018-03-28" version="1">MRARLAAAESRKEHVSSCCPAGHVSTHHHGGDDLATVLIDPFGGGTPSTGIAISNVEVQVLKMNPGWKVSIRRCFGYSERSNRHPLIYNEQDVLNNLGDANAMSIINGGTWTSVLYRVQLPNQSRAVVVKKLQNEGGNAEYAGLTNQCQSEVNLLGSIHHNNIISLEDCFRESNFIVLVYNHKENGSLYQWLHNPADPPSWVHRGGFWTGRRGGASPLVPLKGSTTCTMDATTPSCTTTSTLPAYCLTLTSRPRSHASISHRSALPDMTSRCQSTSPLLSTCLATQLQVDVYSFGVVLPELVTGRVANKAIVDGHLAAWAGRHCNGLMENAGDFSGIVDMAIPDRARYLKEMAAVFKLGVDCTVKEPQERPSMHEVLYRLRNRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYG2</accession>
    <name>A0A2K2CYG2_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g20187v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT67072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CYG2"/>
    <dbReference type="EnsemblPlants" id="PNT67072">
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        <property type="gene ID" value="BRADI_3g20187v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67072">
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        <property type="gene ID" value="BRADI_3g20187v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CYG2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="131"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="243" mass="25995" checksum="48D9D7CDE00315D9" modified="2018-03-28" version="1">MRDRIEPNRPSPNRPATLTDRWDPRVIFNLRPKNPATHARELPTTPPPISPRSGCFFQILARHPEGLDFLLHFLQTPSPISHRLESNCSPEFVAATAVLRVSPPFRLPPLFLAHAVTRVVSDLALRALAAPPSSSAPAAAARRATSGRRPAPPPRPPARAPAPCPRARAPPRARAPPPPAALRALSVPAQPPALAPAARPRAARAARPRCCPRSASARPAPAPRRPAPPARAAAGELARRPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYJ4</accession>
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            <fullName evidence="1">F-box domain-containing protein</fullName>
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        <name evidence="3" type="primary">LOC100832968</name>
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    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571648.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CYJ4"/>
    <dbReference type="STRING" id="15368.A0A2K2CYJ4"/>
    <dbReference type="EnsemblPlants" id="PNT67096">
        <property type="protein sequence ID" value="PNT67096"/>
        <property type="gene ID" value="BRADI_3g20818v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832968"/>
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        <property type="protein sequence ID" value="PNT67096"/>
        <property type="gene ID" value="BRADI_3g20818v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832968"/>
    <dbReference type="OrthoDB" id="673147at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CYJ4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31264:SF3">
        <property type="entry name" value="OS07G0554100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31264">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="12"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="407" mass="45898" checksum="906914E0845238BD" modified="2018-03-28" version="1">MTNQEGMAALTDDLLAAIFLRLPAPADLLRASAVCAAFRRLVTARSFLRRFRSLHAAPFLGFVDRDAFHRALPPHPSAPAARAVSLAGDFSFSFLPTPRDGRWTVLDVRDGRFLLDLGRAPEEEDEYADEEYPAAFTDLAVCDPLQRRCLLLPRIPDTLAAVSVDHPFHVQFGCLCEPFLVPSGGDDDEDEEETSFTVIRIAHSETNLLALVFSSRTGQWRAVASQAWSDLLRGSGVSMGVIFSRQYACNCFYWMVGSTENLLVFDTRRMEFSVACLPPGCYGQNHIAIVDAGESRPGLFSVREHVRDEAVDLCYTVRSSNQWQMEKTIPLDSGYRYYIRGATERYLLLVRCRREMNLRSLLDTRHFEWFSLDFKTLQLENVCVLKHGSLRAHIYTNFPPSLLSPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CZ98</accession>
    <name>A0A2K2CZ98_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g25668v3</name>
    </gene>
    <organism evidence="1">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CZ98"/>
    <dbReference type="EnsemblPlants" id="PNT67346">
        <property type="protein sequence ID" value="PNT67346"/>
        <property type="gene ID" value="BRADI_3g25668v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67346">
        <property type="protein sequence ID" value="PNT67346"/>
        <property type="gene ID" value="BRADI_3g25668v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CZ98"/>
    <dbReference type="OrthoDB" id="10671943at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT67346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="50" mass="5672" checksum="8EE89209E1844452" modified="2018-03-28" version="1">MRAWHAWKMTARLFLGAAGKGRRHEGTRQRKVANGTKRHMMALPAASLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D1I5</accession>
    <name>A0A2K2D1I5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g36493v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
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                <person name="Harmon-Smith M."/>
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                <person name="Bruce D."/>
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    </evidence>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
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                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="2">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="2 3">Bd21</strain>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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        </source>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="PNT76186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="72" mass="8070" checksum="F3474050386BF5F3" modified="2018-03-28" version="1">MHLDSSVTLHQRIRLSLLVAAICRTSNWTKINPGTSLLDHTKFCIMVRPRSCHMFALSSTIAGRSKTHHPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQ89</accession>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT76440"/>
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    </dbReference>
    <dbReference type="Gramene" id="PNT76440">
        <property type="protein sequence ID" value="PNT76440"/>
        <property type="gene ID" value="BRADI_1g48194v3"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
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        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="99" mass="9997" checksum="8290DBB94112B2BE" modified="2018-03-28" version="1">MLPHPSALLQPARAQAQGREHQAASASAAAAPTSIRPGASSTHLGSGRRRAPPRRIRPAPSAPTSMASCCASPRRGGGGGGGGGRRRGSLVVGRERRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <property type="protein sequence ID" value="PNT76849"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT76849.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
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        <name type="common">Purple false brome</name>
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            <taxon>Poaceae</taxon>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DRL3"/>
    <dbReference type="STRING" id="15368.A0A2K2DRL3"/>
    <dbReference type="EnsemblPlants" id="PNT76912">
        <property type="protein sequence ID" value="PNT76912"/>
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        <property type="protein sequence ID" value="PNT76912"/>
        <property type="gene ID" value="BRADI_1g55511v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RBD_domain_sf"/>
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    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
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        <property type="entry name" value="RRM"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="349"/>
            <end position="437"/>
        </location>
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        <location>
            <begin position="436"/>
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            <begin position="43"/>
            <end position="52"/>
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            <begin position="135"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT76912.1"/>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT76912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="472" mass="49521" checksum="5DFB8EE112C26C4A" modified="2018-03-28" version="1" fragment="single">MAPQAGLGVEWADAQEGTDTLWSGVSEVAPPVGPTSREVGTPQDGQQDQISAGRSPPLPSALLPPKKRFLRNASTLIPTPPPPTTTTTNAPAETLVAPTPSPLPPVADSGILPPPPKAAEPSTTPIPIADSKLPSNPPPPTPMAAEPPTPPIPAAEAKLPTTPPSAPGAAAEDPAPERKVQKRKVLKKVIVKVPKGTLAARKAAAAAAAAASADGAGAKRNETLGEKGCNTGCSHLGCKEGQSSIKNCKLAAAAARAREALIKRKQLLLEEQALATKPAIDCNAPVEGVKEGLSEQTLARKPAVDCNAPVEGRKEGLLEQTLARKPAVNCSAAAEEGEGMSEAESMRMKEVFVGGLHYDAKEEDVRAVFRKAGEITQVRVIPNTQTGKNKGYCFVQYREAAHAKKAIEELGNVKICGKHCRTAALDGNNIEKKRKKEDVNIKDSLPKPVEKSKQNREKYTSNISQSDMTVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DSV8</accession>
    <name>A0A2K2DSV8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g61695v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DSV8"/>
    <dbReference type="EnsemblPlants" id="PNT77366">
        <property type="protein sequence ID" value="PNT77366"/>
        <property type="gene ID" value="BRADI_1g61695v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT77366"/>
        <property type="gene ID" value="BRADI_1g61695v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="expression patterns" value="differential"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
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            <begin position="59"/>
            <end position="138"/>
        </location>
    </feature>
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        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT77366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="250" mass="27811" checksum="19024F1C2431B546" modified="2018-03-28" version="1">MEGTRKKGAPSPRRTEREARRSGPSSLAAMENEEGSSLAEPYVAGALVVAAAVDGERTLPTVGNVGAGCGGGDGQDWGREGSGGQDTEEAEENDERKTWSGFCRLLTPYNPSQSGDSGGDRTREGHSAGSEACSSSSGDRLRHRCRFRGRFGYVYQSEAKQDPTETSLNIKQKLYSLIDQSPSNQRLILLATMMYWMIHSHWQIRREQSVRGCFHCKARRLHIALMMHTLLLLYYLMMPVRSCFFYFVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>I1GL52</accession>
    <name>I1GL52_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Cellulose synthase</fullName>
            <ecNumber evidence="23">2.4.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC100833360</name>
        <name evidence="25" type="ORF">BRADI_1g02510v3</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">Bd21</strain>
            <strain evidence="26">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 23">
            <text>[(1-&gt;4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19929"/>
            <dbReference type="Rhea" id="RHEA-COMP:10033"/>
            <dbReference type="Rhea" id="RHEA-COMP:10034"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18246"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="23">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 23">Glycan metabolism; plant cellulose biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 23">Cell membrane</location>
            <topology evidence="2 23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 23">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.12" evidence="23"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562886.1">
        <property type="nucleotide sequence ID" value="XM_003562838.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GL52"/>
    <dbReference type="STRING" id="15368.I1GL52"/>
    <dbReference type="EnsemblPlants" id="KQK12261">
        <property type="protein sequence ID" value="KQK12261"/>
        <property type="gene ID" value="BRADI_1g02510v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833360"/>
    <dbReference type="Gramene" id="KQK12261">
        <property type="protein sequence ID" value="KQK12261"/>
        <property type="gene ID" value="BRADI_1g02510v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833360"/>
    <dbReference type="eggNOG" id="ENOG502QQGG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001418_0_2_1"/>
    <dbReference type="OMA" id="SAMLMFE"/>
    <dbReference type="OrthoDB" id="72851at2759"/>
    <dbReference type="UniPathway" id="UPA00695"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GL52">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016759">
        <property type="term" value="F:cellulose synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009833">
        <property type="term" value="P:plant-type primary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16617">
        <property type="entry name" value="mRING-HC-C4C4_CesA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000031">
        <property type="entry name" value="Cellulose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000009">
        <property type="entry name" value="Cellulose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027934">
        <property type="entry name" value="CES_Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 23" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17 23" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12 23" id="KW-0135">Cellulose biosynthesis</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 23" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 23" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 23" id="KW-1133">Transmembrane helix</keyword>
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    <keyword evidence="10 22 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="274"/>
            <end position="292"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
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            <begin position="304"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="870"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="904"/>
            <end position="925"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
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            <begin position="937"/>
            <end position="956"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="989"/>
            <end position="1012"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1018"/>
            <end position="1040"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1052"/>
            <end position="1072"/>
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        <location>
            <begin position="39"/>
            <end position="85"/>
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        <location>
            <position position="398"/>
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    <feature type="active site" evidence="19">
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        <location>
            <position position="362"/>
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        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
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        <location>
            <position position="368"/>
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            <name>Mn(2+)</name>
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    </evidence>
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    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12261"/>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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    </evidence>
    <sequence length="1092" mass="123616" checksum="8A750668DE432957" modified="2012-06-13" version="1">MEASAGLVAGSHNRNELVVIRRDGEPGQRPLKQHNSRACQICGDDVGLTPDGEPFVACNECAFPVCRDCYEYERREGTQNCPQCKTRFKRLKGCARVPGDEEEEGADDLENEFNWRDRDADSQYVAESMLHAHMTYGRGGDIDGVPQPFMPIPNVPLLTNGQMVDDIPPEQHALVPSFMGGGGKRIHPLPYADPNLPVQPRSMDPSKDLAAYGYGSVAWKERMESWKQKQERLHQTRNDGGKDWGGDGDDADLPLMDEARQPLSRKVAISSSLINPYRMIIIIRLVIVGFFFHYRVMHPVNDAFVLWLISVICEIWFAMSWILDQFPKWFPIERETYLDRLTLRFDKEGQPSQLAPVDFFVSTVDPLKEPPIVTANTILSILAVDYPVDKLSCYVSDDGAAMLTFEGLSETSEFAKKWVPFCKKYSLEPRAPEWYFQQKIDYLKDKVEPNFVRDRRAMKREYEEFKVRINALVAKAQKVPEEGWTMQDGTPWPGNNVRDHPGMIQVFLGQSGGHDVEGNELPRLVYVSREKRPGYDHHKKAGAMNALVRVSAVLTNAPYMLNLDCDHYVNNSKAVKEAMCFMMDPLVGKKVCYVQFPQRFDAIDRHDRYANRNVVFFDINMKGLDGIQGPIYVGTGCVFRRQALYGYDAPKTKKPPSRTCNCWPKWCFCCFCFGNRKNKKKVTKAKTEKKKRFFFKKAENQSPAYALSEIDEAAAGAETEKAGIVNQQKLEKKFGQSSVFVASTLLENGGTLRSASPASLLKEAIHVIGCGYEDKTDWGKEIGWIYGSVTEDILTGFKMHCHGWRSIYCMPKRAAFKGSAPLNLSDRLNQVLRWALGSIEIFFSNHCPLWYGYGGGLKFLERFSYINSIVYPWTSIPLLAYCTLPAICLLTGKFITPELSNLASVWYMSLFICIFVTGILEMRWSHVAVDDWWRNEQFWVIGGVSAHFFAVFQGLLKVIAGVDTSFTVTTKAGDDGEFSELYTFKWTTLLIPPTTLLLLNFIGVVAGISNAINNGYESWGPLFGKLFFAFWVIVHLYPFLKGLLGRQNRTPTIVIVWSILLASILSLLWVRVNPFLAKNDGPLLEQCGLDCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GL66</accession>
    <name>I1GL66_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Electron transfer flavoprotein subunit alpha</fullName>
            <shortName evidence="11">Alpha-ETF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100829269</name>
        <name evidence="14" type="ORF">BRADI_1g02630v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11 12">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562959.1">
        <property type="nucleotide sequence ID" value="XM_003562911.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GL66"/>
    <dbReference type="FunCoup" id="I1GL66">
        <property type="interactions" value="1915"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GL66"/>
    <dbReference type="EnsemblPlants" id="KQK12278">
        <property type="protein sequence ID" value="KQK12278"/>
        <property type="gene ID" value="BRADI_1g02630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829269"/>
    <dbReference type="Gramene" id="KQK12278">
        <property type="protein sequence ID" value="KQK12278"/>
        <property type="gene ID" value="BRADI_1g02630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829269"/>
    <dbReference type="eggNOG" id="KOG3954">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_1_1"/>
    <dbReference type="OMA" id="HHICGIG"/>
    <dbReference type="OrthoDB" id="1715808at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GL66">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000157">
        <property type="entry name" value="Electron transfer flavoprotein subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 11 12" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="13">
        <location>
            <begin position="35"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="274"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="288"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="305"/>
            <end position="312"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000089-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK12278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="360" mass="37840" checksum="0EF95F3B498439FA" modified="2012-06-13" version="1">MVMAAALLAGALRRGRAGGGARYFAGESLRRLVSTLVVAEHEGGLVKPSSLSALAAAEAIAKENKVSLLLGGSGPTLHKAAQHAASSHPLVNEVLVADSDVFAHPLAEPWAELLRSVQQKGGYSHVIASSTSFGKNLLPRAAALLDVSPVTDVTAISEPRVFVRPIYAGNALCTVRYTGENPCMMSIRSTSFSPTTESMPETKVAPITQVDLSFLNEVSSRKSSWVNLTSQDTERPDLANACVVVTGGRGLKSAENFKLLEQLAEKLGAAVGATRAAVDAGYVPNELQVGQTGKIVAPELYIAFGVSGAIQHLAGMRDSKVIVAVNKDADAPIFQVADYGIVGDLFQVLGELLRKMPDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLB6</accession>
    <name>I1GLB6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100831215</name>
        <name evidence="3" type="ORF">BRADI_1g03050v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563218.1">
        <property type="nucleotide sequence ID" value="XM_003563170.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLB6"/>
    <dbReference type="FunCoup" id="I1GLB6">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK12338">
        <property type="protein sequence ID" value="KQK12338"/>
        <property type="gene ID" value="BRADI_1g03050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831215"/>
    <dbReference type="Gramene" id="KQK12338">
        <property type="protein sequence ID" value="KQK12338"/>
        <property type="gene ID" value="BRADI_1g03050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831215"/>
    <dbReference type="eggNOG" id="KOG2088">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020385_1_0_1"/>
    <dbReference type="OMA" id="GARNDKQ"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GLB6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023">
        <property type="entry name" value="LIPASE CLASS 3 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023:SF2">
        <property type="entry name" value="LIPASE FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="195"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK12338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="591" mass="64396" checksum="9C133E8B188C9680" modified="2012-06-13" version="1">MPAAVAAAAAAATSGAALVVYLLITCRPQPPRGAEGAAAAEEESLLSRHAKAGSAGGGAEEEDEEWPHSPPASCCEAAAVAARTARRAWELTVGRWGLHGLIFGINSHMKRQGNLQHEYSGNDCLQLKGHEVHTEVAYLLEYLKLCMFYSKKRFSEFLKFGGYSQKDVLIHKCRARLMRPSFAIVRDQNSKCFLLFIRGAISTKERLTAAASVEVPFHHIVLNEGQIDNVILGYAHYGMLAAARWIANLAIPLLHSAVREFPDYQLKVIGHSMGAGIGAILTYILHEHYDFTSCTCLAFAPPACMTWELAESGKGFITSLVNRNDMVPAFSKVAFESLRSEVMVSSKLDDLQDQDHLSLFAKISQRVALAKSHMLSISHSVGKTADSDPSISEPLLKQAPEIITPEANRLNIDCSQQRADVAANLEHDFSAVSVATSEERIILVDKDDFASAKTVTESCCASQGDVDSNQVLDTEEASLETKEEAPSLTQNGAGKEKQKEPPSGPGSRQLFPPGRIIHMVAQLPLEPNPGEGTSSNEIVSIYETPRDLYSKIRLAPNMIDEHYMTSYISTMESLLEQLQSEDTVSTAPNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLT4</accession>
    <name>I1GLT4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100845335</name>
        <name evidence="5" type="ORF">BRADI_1g04410v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229441.1">
        <property type="nucleotide sequence ID" value="XM_010231139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLT4"/>
    <dbReference type="STRING" id="15368.I1GLT4"/>
    <dbReference type="EnsemblPlants" id="KQK12544">
        <property type="protein sequence ID" value="KQK12544"/>
        <property type="gene ID" value="BRADI_1g04410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845335"/>
    <dbReference type="Gramene" id="KQK12544">
        <property type="protein sequence ID" value="KQK12544"/>
        <property type="gene ID" value="BRADI_1g04410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845335"/>
    <dbReference type="eggNOG" id="ENOG502RXRA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087111_2_0_1"/>
    <dbReference type="OMA" id="MYRIQEM"/>
    <dbReference type="OrthoDB" id="644695at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21495">
        <property type="entry name" value="NUCLEOPORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982576" description="Dirigent protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK12544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="180" mass="19821" checksum="5FFC8E9C5B12280E" modified="2012-06-13" version="1">MRTPALLLLVLVAFQRATATDTDYPLGPEKVTNLHFFMHDTLSGKDPSAVLVARAAGANYTPRPDNLFPFSSVYVFNDVLTEGRERSSRVVGNAHGTYIVTAKNEKTILMAVDYQLADYQNSSFAVFTRNPVGVDGRELTVVGGHGAFRMARGFAILPSHYLNTENGDAILEYNVTLFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMF7</accession>
    <name>I1GMF7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100838415</name>
        <name evidence="9" type="ORF">BRADI_1g06190v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in environmental stress response.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557923.1">
        <property type="nucleotide sequence ID" value="XM_003557875.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMF7"/>
    <dbReference type="STRING" id="15368.I1GMF7"/>
    <dbReference type="EnsemblPlants" id="KQK12822">
        <property type="protein sequence ID" value="KQK12822"/>
        <property type="gene ID" value="BRADI_1g06190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838415"/>
    <dbReference type="Gramene" id="KQK12822">
        <property type="protein sequence ID" value="KQK12822"/>
        <property type="gene ID" value="BRADI_1g06190v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838415"/>
    <dbReference type="eggNOG" id="KOG3173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057016_5_3_1"/>
    <dbReference type="OMA" id="CGAAMCA"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF136">
        <property type="entry name" value="AN1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="A20-type" evidence="7">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="8">
        <location>
            <begin position="101"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK12822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="17221" checksum="47ADE0434C89ED59" modified="2012-06-13" version="1">MAQQGAATAQKRKCPSDEESTAAGLCANGCGFFGAAATANMCSKCYRDHVAADGTTPSVYFAPAAAASSSTATPEKKVKISAAVDASSDAPAAVDPASVKPAAANRCATCRKKVGILGFRCRCEGTFCSVHRYADKHACGFDYKTAAQEQIATQNPVVVADKMVTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMS4</accession>
    <name>I1GMS4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Erythronate-4-phosphate dehydrogenase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100843908</name>
        <name evidence="2" type="ORF">BRADI_1g07070v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558612.1">
        <property type="nucleotide sequence ID" value="XM_003558564.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMS4"/>
    <dbReference type="STRING" id="15368.I1GMS4"/>
    <dbReference type="EnsemblPlants" id="KQK12962">
        <property type="protein sequence ID" value="KQK12962"/>
        <property type="gene ID" value="BRADI_1g07070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843908"/>
    <dbReference type="Gramene" id="KQK12962">
        <property type="protein sequence ID" value="KQK12962"/>
        <property type="gene ID" value="BRADI_1g07070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843908"/>
    <dbReference type="eggNOG" id="ENOG502QQ72">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080009_0_0_1"/>
    <dbReference type="OMA" id="TRCSMYS"/>
    <dbReference type="OrthoDB" id="2016101at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42938:SF11">
        <property type="entry name" value="ERYTHRONATE-4-PHOSPHATE DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42938">
        <property type="entry name" value="FORMATE DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK12962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="33551" checksum="BD96503EDB7BA89E" modified="2012-06-13" version="1">MGDGMAFEAARKIIMHPLYASRSSPWLDLKVFYVRVSNCVVDESAPERLTLNHIPLSPDTVIEVNGRRSSMHTEFISSSLRRDRIDTKTEEATYVSTDSIRMTGSVRFQVFDKNDLLLTGDLELCNANGVVGESKNSSRNWNIKCQSSASSSGFLKGKMSTGPDSVHPVVEVYVAGTFSGTPIILTKTIQFISRRKSQMKLKLDSIPENEATEQKENLHRDSLKVSEFQDPKSDTDVDVDYNSLYSRHDFLEGEDGELSWFNAGVRVGVGIGLGICVGVGLGVGLLVRTYQSTSRNFRRRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNC7</accession>
    <name>I1GNC7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100827932</name>
        <name evidence="11" type="ORF">BRADI_1g08780v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230001.1">
        <property type="nucleotide sequence ID" value="XM_010231699.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNC7"/>
    <dbReference type="FunCoup" id="I1GNC7">
        <property type="interactions" value="2453"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GNC7"/>
    <dbReference type="EnsemblPlants" id="KQK13235">
        <property type="protein sequence ID" value="KQK13235"/>
        <property type="gene ID" value="BRADI_1g08780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827932"/>
    <dbReference type="Gramene" id="KQK13235">
        <property type="protein sequence ID" value="KQK13235"/>
        <property type="gene ID" value="BRADI_1g08780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827932"/>
    <dbReference type="eggNOG" id="ENOG502QUYB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024424_0_0_1"/>
    <dbReference type="OMA" id="GHCIQRG"/>
    <dbReference type="OrthoDB" id="1145453at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GNC7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055508">
        <property type="entry name" value="DUF7081"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056034">
        <property type="entry name" value="DUF7615"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032881">
        <property type="entry name" value="Oberon-like_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33345">
        <property type="entry name" value="ADAPTER PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33345:SF6">
        <property type="entry name" value="OS03G0747200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23299">
        <property type="entry name" value="DUF7081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24590">
        <property type="entry name" value="DUF7615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07227">
        <property type="entry name" value="PHD_Oberon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="8">
        <location>
            <begin position="31"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7081" evidence="9">
        <location>
            <begin position="253"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Oberon-like PHD finger" evidence="7">
        <location>
            <begin position="374"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7615" evidence="10">
        <location>
            <begin position="597"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK13235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="711" mass="80335" checksum="49A5CD9DB0EF9D90" modified="2012-06-13" version="1">MLSTSTDYLHSGGNDINGGNGSRPLGRECVSWTEEMSGYLIDALLHQQVIGNRTAEGRFHTTAFDNVINLVSGKFGVNIDRSHIKNRLKHVKDTFHECESLFGKESGIKWNQGTRRFHADPQVWREFIERKPEAKRWMTKTINHYDRLLELFGKDRERSPAVSSPKVNPKQKAQIEPSKERPPDKKVRVEPPKERSGRQWMPSDGLQLDVVQSSNQPLNAGEVRAEVVTEKNIPRELHLSELCRSENGLVAIPVCANAYGKGLPYAPENWPLDGDEWYWKVGNRSAAGGHWVDRYLIPPSRFRDATGKKTSFTSRLKVEEFIKKDFPDVDPSTFFSKFIWKIPAEESINQRGTQQVRLHEPESGFPDPAGPCKARNNSCNLEREGFIESSALHDCQICCPEPNFCRECCCILCGRVVDNSFGGYSYIKCKEVVKDNYICGHVAHLDCALRCYMTGTVGGTIGLDVQYYCRWCDKKTNLMMHVEKLLETCRSLESRDEIEPILNMGLCILRGSEQAKAKDLENYMGSALAKMKSGVDLAEVWKIENGDGMSIPSSGELSPPITDVTLLGVEQKYPYLSDPLAANEAVENLPIFITGDHNVMSAKFENEIDRALLELKRSQEAEFRLAEQRLYSQRDYILSLYRQLESDRSELADPKPLSDSSRYNVLVSNITTLVNQAKREEEKFKAMLKVSKGFGKTSAKVVQEHFGLSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1GND7</accession>
    <name>I1GND7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100838235</name>
        <name evidence="5" type="ORF">BRADI_1g08880v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559747.1">
        <property type="nucleotide sequence ID" value="XM_003559699.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GND7"/>
    <dbReference type="STRING" id="15368.I1GND7"/>
    <dbReference type="EnsemblPlants" id="KQK13248">
        <property type="protein sequence ID" value="KQK13248"/>
        <property type="gene ID" value="BRADI_1g08880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838235"/>
    <dbReference type="Gramene" id="KQK13248">
        <property type="protein sequence ID" value="KQK13248"/>
        <property type="gene ID" value="BRADI_1g08880v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838235"/>
    <dbReference type="eggNOG" id="ENOG502QVJH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049517_1_0_1"/>
    <dbReference type="OMA" id="GNWEVKC"/>
    <dbReference type="OrthoDB" id="671439at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF316">
        <property type="entry name" value="PROTEIN ECERIFERUM 26-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK13248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="466" mass="48476" checksum="9C66CD519A25D54A" modified="2012-06-13" version="1">MPTAAAATAAGMATGPGSRVTRYAKSTAASVTPVRPGKTHALSALDNAMEWHAVHLVLYCRAAPGVGRDALKESLSEALSLYPAMTGRLVRAAQGEEGGAAGGWIVKCNDAGVRTVDATAAVTLDEWLATASADEEMDLAYFEQMGAEPYIWSPFYVQLTEFTDKSYALGLSCTHVHNDPTAASLFFHGWAAAHRRATSPYPPFLHSPSFAVSPTTPPSPPPLLAAKSTAAPAPPNSDAAMSSATFHFPAAAMRALLSSLEPGATPFAALAALFWLRVAGHDAAESRQKELTLALDFRKKMRAPLPTGYYGSAVHFTRARADLSSPLAAVAAALDACVAGVPEEELWAALEWLHARQASAPGGGGGGEPFQMYGPELTCVALDHVPMYGTEFVAGEPPARVACRVGGAEGEGVVIVMPAAEGGEARDVVVTLPADAAARVCRDGELLRYGARVVAGSKVETGPKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQF5</accession>
    <name>I1GQF5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100845032</name>
        <name evidence="2" type="ORF">BRADI_1g15100v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752040.1">
        <property type="nucleotide sequence ID" value="XM_014896554.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQF5"/>
    <dbReference type="STRING" id="15368.I1GQF5"/>
    <dbReference type="EnsemblPlants" id="KQK14276">
        <property type="protein sequence ID" value="KQK14276"/>
        <property type="gene ID" value="BRADI_1g15100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845032"/>
    <dbReference type="Gramene" id="KQK14276">
        <property type="protein sequence ID" value="KQK14276"/>
        <property type="gene ID" value="BRADI_1g15100v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845032"/>
    <dbReference type="eggNOG" id="ENOG502QUPJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076222_1_0_1"/>
    <dbReference type="OMA" id="MCESARP"/>
    <dbReference type="OrthoDB" id="1667873at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47000:SF11">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASE-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47000">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="68"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="246" mass="25985" checksum="299557EEF7FAEB36" modified="2012-06-13" version="1">MGRRLPALCRGRAATRVRKRVSYCSSKLLPAVTSCGSSTIKNAAAAGAGGWYGGNGAAASMVGMGGGRRVMVVADGRAEAVGALEWALSQAVRSNDAVLLLAVVKPDLADAGADGCVKMSRTRCYEHLDAMRSLCESTRPEVRVEVCVVEAEERAPAVVDAARRHGASLLVLGQSRRAATARWIMGLWPAAAKRQCSSSGGRRRGIGGLVEHCIEHAPCEALGVRRRSSGGYLVSSKRHKDFWLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRD0</accession>
    <name>I1GRD0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100841504</name>
        <name evidence="1" type="ORF">BRADI_1g18420v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562488.1">
        <property type="nucleotide sequence ID" value="XM_003562440.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRD0"/>
    <dbReference type="STRING" id="15368.I1GRD0"/>
    <dbReference type="EnsemblPlants" id="KQK14755">
        <property type="protein sequence ID" value="KQK14755"/>
        <property type="gene ID" value="BRADI_1g18420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841504"/>
    <dbReference type="Gramene" id="KQK14755">
        <property type="protein sequence ID" value="KQK14755"/>
        <property type="gene ID" value="BRADI_1g18420v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841504"/>
    <dbReference type="eggNOG" id="ENOG502QW64">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_827279_0_0_1"/>
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    <dbReference type="OrthoDB" id="10017101at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37886:SF1">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK14755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38226" checksum="C5FCECF5EB3A7B5D" modified="2012-06-13" version="1">MALLSPGSPTARLLTVPSLLLLLSCAALLVFLLLPGLSPSPSPSAGAHLCACADPVTTHTTTSVTTTTTTASPAPVATSPDDVAWLKAQLASNSLALLLASAGDDAWHRLRKGINPRTRQQQLFDINRHHGISHYPDEEATNHTALPCPGELLVEEHHSNYGEPWAGGRDVFEFLANASALVPRDQVLEIGCGTLRVGLHFIRFLETGRFHCLERDELSLMAALRYELPSQGLLYKRPMIVRGEDMDFSKFGDTVMYDLIYASAVFLHIPDKLVWTGLERLAGKLRPQRGRIFVSHNIKFCSRLGGDECTRRLAELGLEYVGKHTHDSLLFNHYEIWFEFRRPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWB0</accession>
    <name>I1GWB0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g33075v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWB0"/>
    <dbReference type="FunCoup" id="I1GWB0">
        <property type="interactions" value="313"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK17213">
        <property type="protein sequence ID" value="KQK17213"/>
        <property type="gene ID" value="BRADI_1g33075v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17213">
        <property type="protein sequence ID" value="KQK17213"/>
        <property type="gene ID" value="BRADI_1g33075v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R66B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2389404_0_0_1"/>
    <dbReference type="InParanoid" id="I1GWB0"/>
    <dbReference type="OMA" id="ACRHDAK"/>
    <dbReference type="OrthoDB" id="669818at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK17213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="91" mass="10136" checksum="7CF33E018ED195E5" modified="2012-06-13" version="1">MCDGRECKRESAVRTSLRRLHGNNTGEPHYYTHAPPAAAAAPRAEADDDNAVEATRASSWKQAGQREMTPRRTAEIARTEKLLHLLLWGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWE2</accession>
    <name>I1GWE2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100828467</name>
        <name evidence="12" type="ORF">BRADI_1g33340v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-homoarginine + 2-oxoglutarate + O2 = 6-hydroxy-L-homoarginine + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:79839"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:143006"/>
            <dbReference type="ChEBI" id="CHEBI:231270"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563457.1">
        <property type="nucleotide sequence ID" value="XM_003563409.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWE2"/>
    <dbReference type="FunCoup" id="I1GWE2">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK17264">
        <property type="protein sequence ID" value="KQK17264"/>
        <property type="gene ID" value="BRADI_1g33340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828467"/>
    <dbReference type="Gramene" id="KQK17264">
        <property type="protein sequence ID" value="KQK17264"/>
        <property type="gene ID" value="BRADI_1g33340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828467"/>
    <dbReference type="eggNOG" id="KOG0143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="ARETGFF"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GWE2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000024">
        <property type="entry name" value="Probable 2-oxoglutarate-dependent dioxygenase At3g49630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="11">
        <location>
            <begin position="181"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK17264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="328" mass="36746" checksum="A9F58C3774362FEA" modified="2012-06-13" version="1">MGSDFKAIPLIDIGPLVEKIDDPDMVGDEGLLGVVRKLDDACREAGFFYVKGHGIAESLMTEVRDVTRKFFQLPDEEKLKIKMTPQSGYRGYQRVGENVTKGKPDMHEAIDCYTPIEPGKYGDLAKPMEGSNLWPDYPSNFKVLLEEYLSLVRDLSRKIMRGIALALGAPLDAFEGGMAGDAFWVFRLIGYPVSADIPQEQRTDIGCGAHTDYGLLTLVNQDDDICALEVRNQSGEWIYAKPVPGTFVCNIGDMLKVWSNGIYQPTLHRVVNNSPRYRVSVAFFYESNFDTTVEPVEFCREKTGGVAKYEKVVYGEHLVQKVLTNFVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXC2</accession>
    <name>I1GXC2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Exopolygalacturonase</fullName>
            <ecNumber evidence="8">3.2.1.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Galacturan 1,4-alpha-galacturonidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Pectinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100831111</name>
        <name evidence="19" type="synonym">LOC100830804</name>
        <name evidence="17" type="ORF">BRADI_1g36090v3</name>
        <name evidence="18" type="ORF">BRADI_1g36110v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl](n) + H2O = alpha-D-galacturonate + [(1-&gt;4)-alpha-D-galacturonosyl](n-1)</text>
            <dbReference type="Rhea" id="RHEA:14117"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14572"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58658"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.2.1.67"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.67" evidence="8"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563631.1">
        <property type="nucleotide sequence ID" value="XM_003563583.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563632.1">
        <property type="nucleotide sequence ID" value="XM_003563584.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXC2"/>
    <dbReference type="STRING" id="15368.I1GXC2"/>
    <dbReference type="EnsemblPlants" id="KQK17684">
        <property type="protein sequence ID" value="KQK17684"/>
        <property type="gene ID" value="BRADI_1g36090v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK17686">
        <property type="protein sequence ID" value="KQK17686"/>
        <property type="gene ID" value="BRADI_1g36110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830804"/>
    <dbReference type="GeneID" id="100831111"/>
    <dbReference type="Gramene" id="KQK17684">
        <property type="protein sequence ID" value="KQK17684"/>
        <property type="gene ID" value="BRADI_1g36090v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17686">
        <property type="protein sequence ID" value="KQK17686"/>
        <property type="gene ID" value="BRADI_1g36110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830804"/>
    <dbReference type="KEGG" id="bdi:100831111"/>
    <dbReference type="eggNOG" id="ENOG502QRSR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016031_2_2_1"/>
    <dbReference type="OMA" id="CSAVKPC"/>
    <dbReference type="OrthoDB" id="640420at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047911">
        <property type="term" value="F:galacturan 1,4-alpha-galacturonidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000004">
        <property type="entry name" value="Pectin lyase-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094172" description="Exopolygalacturonase" evidence="16">
        <location>
            <begin position="23"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK17684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK17686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="412" mass="43239" checksum="0D0E55580C734BBD" modified="2012-06-13" version="1">MAFRNDAIRAFFLLMVVSAAYAKAAEKEAAGSAAAASGPGATLDIVKLGATGDGKTDSTKAVEEAWASACGGTGKQTILIPKGDFLTGPLNFTGPCTGDITIQVDGNLLGSNDLAKYKANWIEIMRVKNLVITGKGKLDGQGPAVWGKNSCAKNYNCKILPNTLVLDFCNNTLIEGITLLNSKFFHLNIYECRGITVKDVTITSPGDSPNTDGIHMGDASNITITDTKIGTGDDCISIGPGTSGVNISGVNCGPGHGISVGSLGRYKDEKDVTDITVKNCVLNKSTNGVRIKSYEDAKSPLVASKLTYENIKMMDVGYPIIIDQKYCPNKICPPKPNSAKVTVKDVTFRNITGTSSTPEAVSLLCSDKQPCSGVQMFDVKVEYSGTNNKTMAVCTNAKVTAKGCSEALACAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZU1</accession>
    <name>I1GZU1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g45945v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZU1"/>
    <dbReference type="EnsemblPlants" id="KQK19014">
        <property type="protein sequence ID" value="KQK19014"/>
        <property type="gene ID" value="BRADI_1g45945v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19014">
        <property type="protein sequence ID" value="KQK19014"/>
        <property type="gene ID" value="BRADI_1g45945v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1515">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_22_0_1"/>
    <dbReference type="InParanoid" id="I1GZU1"/>
    <dbReference type="OMA" id="TLWRVMC"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF541">
        <property type="entry name" value="OS06G0214800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="1">
        <location>
            <begin position="55"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK19014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="307" mass="32135" checksum="C22BC53C3C42DB33" modified="2012-06-13" version="1">MNSAPIPAGVDPATGVASKDVVINQATGLWARVFLPPPGSGHHAAPAGGSKLPVVVYYHGGAFVLGSAAAPTTHGYLNCLVAAANVVAVAVEYRLAPEHPLPAAFDDSWEGLKWVVASHAIATATGDAGCETWLVNHGDFARVFLAGSSAGGTIAHAMAVRAGGPGVGISIRGVLVMHPFFSGVADIGKETTKGKAWTDAFWRFRHPAAPLGLDDPLANPFSEAAGGSAARVAGERVLVCVAEKDTLRDRGVWYYENLKASGYGGEVELFESMGEGHVFHCINPRSEKAIKMQERVVRVLRPRLDTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1H362</accession>
    <name>I1H362_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100838413</name>
        <name evidence="5" type="ORF">BRADI_1g55690v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557496.1">
        <property type="nucleotide sequence ID" value="XM_003557448.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H362"/>
    <dbReference type="STRING" id="15368.I1H362"/>
    <dbReference type="EnsemblPlants" id="KQK20620">
        <property type="protein sequence ID" value="KQK20620"/>
        <property type="gene ID" value="BRADI_1g55690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838413"/>
    <dbReference type="Gramene" id="KQK20620">
        <property type="protein sequence ID" value="KQK20620"/>
        <property type="gene ID" value="BRADI_1g55690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838413"/>
    <dbReference type="eggNOG" id="KOG2241">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_6_2_1"/>
    <dbReference type="OMA" id="EGHDMPY"/>
    <dbReference type="OrthoDB" id="19141at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H362">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02799">
        <property type="entry name" value="tRNA_bind_EMAP-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000225">
        <property type="entry name" value="tyrosine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF47">
        <property type="entry name" value="NUCLEIC ACID-BINDING, OB-FOLD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="4">
        <location>
            <begin position="92"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK20620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="260" mass="27787" checksum="D778E943C3A46DD4" modified="2012-06-13" version="1">MMAAARMSFASCVRTHLLLPSTPSSPILARTPRVGFGGLAKSTSFLPLPFAARSTNHAALFCSSSSSPAEAVVAPAAVAAEEEKREVSAGELAGLLDIRVGRIVKAWMHPEADTLYVEEVDVGEEQPRTICSGLVKYLSLDQLQDSSVIVLANLKPRNMRGIKSNGMLMCASDASHETVELLTPPEGSVPGERVWFGTDDEKTLQSEPATANQVQKKKIWEAVQPHLKTTDNCIVVLGEHSMRTSAGKVFCKSLQGAGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1H3F3</accession>
    <name>I1H3F3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">33 kDa subunit of oxygen evolving system of photosystem II</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">33 kDa thylakoid membrane protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">OEC 33 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100827507</name>
        <name evidence="11" type="ORF">BRADI_1g56580v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PsbO family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557544.1">
        <property type="nucleotide sequence ID" value="XM_003557496.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H3F3"/>
    <dbReference type="FunCoup" id="I1H3F3">
        <property type="interactions" value="878"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H3F3"/>
    <dbReference type="EnsemblPlants" id="KQK20742">
        <property type="protein sequence ID" value="KQK20742"/>
        <property type="gene ID" value="BRADI_1g56580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827507"/>
    <dbReference type="Gramene" id="KQK20742">
        <property type="protein sequence ID" value="KQK20742"/>
        <property type="gene ID" value="BRADI_1g56580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827507"/>
    <dbReference type="eggNOG" id="ENOG502QRXA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063138_0_0_1"/>
    <dbReference type="OMA" id="GKANDCP"/>
    <dbReference type="OrthoDB" id="2860at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030095">
        <property type="term" value="C:chloroplast photosystem II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009654">
        <property type="term" value="C:photosystem II oxygen evolving complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010242">
        <property type="term" value="F:oxygen evolving activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010207">
        <property type="term" value="P:photosystem II assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042549">
        <property type="term" value="P:photosystem II stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2050.10:FF:000001">
        <property type="entry name" value="Oxygen-evolving enhancer protein 1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2050.10">
        <property type="entry name" value="photosynthetic oxygen evolving center domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.30">
        <property type="entry name" value="Photosystem II, cytochrome c-550 precursor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_B-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002628">
        <property type="entry name" value="PsbO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34058">
        <property type="entry name" value="OXYGEN-EVOLVING ENHANCER PROTEIN 1-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01716">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="OMPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="7" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0793">Thylakoid</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK20742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="330" mass="34580" checksum="7117D413050901CC" modified="2012-06-13" version="1">MAASLQAAATLMQPAKMGRSASSSSAVLLPSSARPSSHAARTISCSLTSDLREVATKCADAAKLAGFALATSALLVSGASAEGAPKRLTFDEIQSKTYMEVKGTGTANQCPTIDGGVDAFPFKAGKYEMKKFCLEPTSFTVKAEGIAKNAPPEFQKTKLMTRLTYTLDEIEGPLEVAADGTLKFEEKDGIDYAAVTVQLPGGERVPFLFTVKQLVATGKPESFSGPFLVPSYRGSSFLDPKGRGGSTGYDNAVALPAGGRGDEEELAKENVKNASSSQGNITLSVTKSKPETGEVIGVFESVQPSDTDLGAKAPKDVKIQGVWYAQLESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4P3</accession>
    <name>I1H4P3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Thymidine kinase</fullName>
            <ecNumber evidence="2 11">2.7.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100835449</name>
        <name evidence="13" type="ORF">BRADI_1g60190v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>thymidine + ATP = dTMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19129"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the thymidine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.21" evidence="2 11"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557737.1">
        <property type="nucleotide sequence ID" value="XM_003557689.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H4P3"/>
    <dbReference type="FunCoup" id="I1H4P3">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H4P3"/>
    <dbReference type="EnsemblPlants" id="KQK21323">
        <property type="protein sequence ID" value="KQK21323"/>
        <property type="gene ID" value="BRADI_1g60190v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK21324">
        <property type="protein sequence ID" value="KQK21324"/>
        <property type="gene ID" value="BRADI_1g60190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835449"/>
    <dbReference type="Gramene" id="KQK21323">
        <property type="protein sequence ID" value="KQK21323"/>
        <property type="gene ID" value="BRADI_1g60190v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21324">
        <property type="protein sequence ID" value="KQK21324"/>
        <property type="gene ID" value="BRADI_1g60190v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835449"/>
    <dbReference type="eggNOG" id="KOG3125">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064400_1_1_1"/>
    <dbReference type="OMA" id="EAYEPRC"/>
    <dbReference type="OrthoDB" id="439028at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004797">
        <property type="term" value="F:thymidine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010225">
        <property type="term" value="P:response to UV-C"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046104">
        <property type="term" value="P:thymidine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000051">
        <property type="entry name" value="Thymidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000948">
        <property type="entry name" value="Thymidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001267">
        <property type="entry name" value="Thymidine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020633">
        <property type="entry name" value="Thymidine_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11441">
        <property type="entry name" value="THYMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11441:SF0">
        <property type="entry name" value="THYMIDINE KINASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00265">
        <property type="entry name" value="TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00603">
        <property type="entry name" value="TK_CELLULAR_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK21323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="279" mass="30416" checksum="6D935CC0B4C0D68F" modified="2012-06-13" version="1">MRSVCAMTALLSVCSSTSLRSSAFIPRAPFLSLPFRRDLSCSKLRLARSISTAAAAVKARAGGGRPLMEVRAAQSGEIHVIVGPMFAGKTTALIRRVQAEAATGRTVALIKSDKDNRYGLDSAVTHDGTKMACWALPKLSSFQDKLGTEAYDKVDVIGIDEAQFFDDLYDFCCKAADHDGKIVVVAGLDGDYKRNKFGSVLDIIPLADSVTKLTARCELCGHRAFFTLRKTQETRTEFIGGADVYMPVCRQHYLDGQIVIEATRIVMEVERSTVTHALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6J7</accession>
    <name>I1H6J7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100840490</name>
        <name evidence="5" type="ORF">BRADI_1g65480v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228750.1">
        <property type="nucleotide sequence ID" value="XM_010230448.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6J7"/>
    <dbReference type="FunCoup" id="I1H6J7">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H6J7"/>
    <dbReference type="EnsemblPlants" id="KQK22151">
        <property type="protein sequence ID" value="KQK22151"/>
        <property type="gene ID" value="BRADI_1g65480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840490"/>
    <dbReference type="Gramene" id="KQK22151">
        <property type="protein sequence ID" value="KQK22151"/>
        <property type="gene ID" value="BRADI_1g65480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840490"/>
    <dbReference type="eggNOG" id="KOG0714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="FFISYND"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H6J7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009860">
        <property type="term" value="P:pollen tube growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000045">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052842">
        <property type="entry name" value="ER_Co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45184">
        <property type="entry name" value="DNAJ PROTEIN ERDJ3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45184:SF1">
        <property type="entry name" value="DNAJ PROTEIN ERDJ3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094380" description="J domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="28"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK22151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="580" mass="63261" checksum="538E1D2C4DA419A8" modified="2012-06-13" version="1">MGISIRSLIVASLILNSIALVLAAKTLDPYKVLGVDKNASQRDIKKAFHKLSLKYHPDKNKGKGAQEKFEEINNAHEILSDEEKRKNYDLYGDEKGNPGFGGGNFGSRDGHTHFTGGGPKTTYFSSGDGWQTMGGQGDSKTFSFSFGGHPGAGGANPFGFDVGDVFANMFAGGSMGGSQHGGSAGSARRSARTSSQHSNSVTIPEVTMETFNKEVADQGVTWLLLFYKQHAKDQFVVESIMEDVARSLDGAVRAGKVNCNNEKALCKKTGVSVGKSARLFIYSYSTTEKGSLHEYSGDNDAKNLKTFCQEHLPRFSRRVDISQFSFPSNAFSNLPQVLLLSTKKDTPVMWRAVSGMFRNRLIFYDAEVQDVSLPLLKRLGVKNVPALIGRSVNGEEHLLKDGISVKNLSSGIKELKTLLESFEKKNKKLASSQPKKPSKTSEPKENKIPLLTASNYGEICGEKASVCIIGVFKSNKAKEKLEIILSEISQKTLIRGTSYNSGNPIAYALLDAGKQSAFLSSFDKSAYKSSDKLLLAYKPRRGRFAAYNGEVTVEEAERFVASVLNGDVQFSATRQKPVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6V4</accession>
    <name>I1H6V4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC104581807</name>
        <name evidence="12" type="ORF">BRADI_1g66330v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228788.1">
        <property type="nucleotide sequence ID" value="XM_010230486.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6V4"/>
    <dbReference type="FunCoup" id="I1H6V4">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H6V4"/>
    <dbReference type="EnsemblPlants" id="KQK22299">
        <property type="protein sequence ID" value="KQK22299"/>
        <property type="gene ID" value="BRADI_1g66330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581807"/>
    <dbReference type="Gramene" id="KQK22299">
        <property type="protein sequence ID" value="KQK22299"/>
        <property type="gene ID" value="BRADI_1g66330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104581807"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059471_3_3_1"/>
    <dbReference type="OMA" id="SGHHYQH"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26374:SF471">
        <property type="entry name" value="OS03G0279700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26374">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK22299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="198" mass="20829" checksum="DDE147EAE30EB9A3" modified="2012-06-13" version="1">MAHGKRSRQQAEAAAAGVSLLDLDSGDMARILMLFSGHNHQHHIEQHGFAAPSSSPERVFECKTCSRQFPSFQALGGHRASHKKPRLADGAQSDPPKPKVHGCSVCGLEFAVGQALGGHMRRHRAAVAADGMGLGLGLGLGLGLGPKVDSGKKTTHAAELVLDLNVPALEEEPAAVGVDRARTGLAAELFPVVVDFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9I8</accession>
    <name>I1H9I8_BRADI</name>
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        <recommendedName>
            <fullName evidence="16">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100828864</name>
        <name evidence="13" type="ORF">BRADI_1g74547v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751834.1">
        <property type="nucleotide sequence ID" value="XM_014896348.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H9I8"/>
    <dbReference type="EnsemblPlants" id="KQK23552">
        <property type="protein sequence ID" value="KQK23552"/>
        <property type="gene ID" value="BRADI_1g74547v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828864"/>
    <dbReference type="Gramene" id="KQK23552">
        <property type="protein sequence ID" value="KQK23552"/>
        <property type="gene ID" value="BRADI_1g74547v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828864"/>
    <dbReference type="eggNOG" id="ENOG502QVIY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000625_4_3_1"/>
    <dbReference type="OMA" id="TMTIKRT"/>
    <dbReference type="OrthoDB" id="1922155at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094450" description="Subtilisin-like protease" evidence="9">
        <location>
            <begin position="24"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="11">
        <location>
            <begin position="31"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="10">
        <location>
            <begin position="138"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="12">
        <location>
            <begin position="677"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="786"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK23552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="808" mass="85738" checksum="D679E4B76F372061" modified="2012-06-13" version="1">MAALLLLLGVFSVLFAAIYPVQSTPASHSHVHIVYLGHNNGLSPSLTAQFHLRLLSRVFSGPDEARQAILYNYSYGFSGFAAVLNSTQADTLSETEEVISVFRSRMLQLHTTRSWDFMGISLHLQMQQSSSRVHLKYGADVIVGILDTGVWPESESFRDDPHLGPVPSSWRGTCVGGQQFDPATACNRKLIGARYYLAGFESDLGPLNTSGGAEYRSPRDRVGHGTHTASTAVGSVSPNSSYFGGLGRGAVRGGAPGARLAVYKVCWFKDMTGRCSDADILAAFDDALHDGVHVISASLGSPPPLSPLFETSTEVGSFHAMQLGVSTVFSAGNDGPDAAMVQNVSPWALTVAASTIDRRFPTVITLGNNASFVGESFVVEDMKMPLVESSSVFTDGTCTFDQLVNRTAASGKMVLCFSTMGMVSSEGAALAVYAGGGSGVIFADTITRKSTQDNFLPTVHVDLRQGTQILDYIHVRSRQPASVHVSPSRTVVGRTPAPAVAYFSSRGPSSISPNILKPDVTAPGVNILAAWPPKSPPTVLPLDKRSTHWNFDTGTSMSCPHVSGIVALLKSIHPAWSPTAVKSALMTTAYMLDNTGDAMQAGGTLKAADAFDAGAGHVDPLRALDPGLVYDADARDHVRFLCGLGYTSAQVRRMVLPSPSINATCPSGPSAETEADDLNYPAIVLADLGGTRTVKRTLTNVGPRRDVVYWASAVVSPQGARVEVWPRELAFCAGRDWAEFYVTVTPGKMSRGRFDFGEIVWSDAFRHRVRTPLVVSVANLPDNGVGAGSGARSMDGHGAADPPSQAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9M7</accession>
    <name>I1H9M7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100822166</name>
        <name evidence="3" type="ORF">BRADI_1g74910v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558794.1">
        <property type="nucleotide sequence ID" value="XM_003558746.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H9M7"/>
    <dbReference type="STRING" id="15368.I1H9M7"/>
    <dbReference type="EnsemblPlants" id="KQK23610">
        <property type="protein sequence ID" value="KQK23610"/>
        <property type="gene ID" value="BRADI_1g74910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822166"/>
    <dbReference type="Gramene" id="KQK23610">
        <property type="protein sequence ID" value="KQK23610"/>
        <property type="gene ID" value="BRADI_1g74910v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822166"/>
    <dbReference type="eggNOG" id="KOG0092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_2_1"/>
    <dbReference type="OMA" id="SESHRTC"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000851">
        <property type="entry name" value="Ras-related small GTP-binding family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK23610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="199" mass="21687" checksum="B3FDFE97867DC17E" modified="2012-06-13" version="1">MGCSSSVPARSTGGLNNINDNSTATDSNELRAKLVLLGDSGVGKSCIVLRFVRGQFDPTSKVTVGASFLSQTLALEDSTIVKFEIWDTAGQERYAALAPLYYRGAAAAIVVYDITSPESFKKAQYWVKELQKHGNPGIIMVLVGNKADLHESRSVPTQEAQEYAEKNSMFFMETSAKTADNINQLFEEIAKRLPRPTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9S3</accession>
    <name>I1H9S3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100840494</name>
        <name evidence="6" type="ORF">BRADI_1g75280v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CKS family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229218.1">
        <property type="nucleotide sequence ID" value="XM_010230916.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229219.1">
        <property type="nucleotide sequence ID" value="XM_010230917.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H9S3"/>
    <dbReference type="STRING" id="15368.I1H9S3"/>
    <dbReference type="EnsemblPlants" id="KQK23666">
        <property type="protein sequence ID" value="KQK23666"/>
        <property type="gene ID" value="BRADI_1g75280v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK23667">
        <property type="protein sequence ID" value="KQK23667"/>
        <property type="gene ID" value="BRADI_1g75280v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840494"/>
    <dbReference type="Gramene" id="KQK23666">
        <property type="protein sequence ID" value="KQK23666"/>
        <property type="gene ID" value="BRADI_1g75280v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK23667">
        <property type="protein sequence ID" value="KQK23667"/>
        <property type="gene ID" value="BRADI_1g75280v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840494"/>
    <dbReference type="eggNOG" id="KOG3484">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140546_2_0_1"/>
    <dbReference type="OMA" id="MSENEWR"/>
    <dbReference type="OrthoDB" id="440676at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000003">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00944">
        <property type="entry name" value="CKS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 5" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK23666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="90" mass="10591" checksum="9F548F919288F480" modified="2012-06-13" version="1">MGQIQYSEKYFDDTFEYRHVVLPPEVAKLLPKNRLLAENEWRALGVQQSRGWVHYAVHRPEPHIMLFRRPLNYQQQQEAAAAAAAQMMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAN3</accession>
    <name>I1HAN3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g77860v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAN3"/>
    <dbReference type="EnsemblPlants" id="KQK24049">
        <property type="protein sequence ID" value="KQK24049"/>
        <property type="gene ID" value="BRADI_1g77860v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK24049">
        <property type="protein sequence ID" value="KQK24049"/>
        <property type="gene ID" value="BRADI_1g77860v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R64H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184794_0_0_1"/>
    <dbReference type="InParanoid" id="I1HAN3"/>
    <dbReference type="OMA" id="SSRRMMQ"/>
    <dbReference type="OrthoDB" id="663321at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094488" evidence="2">
        <location>
            <begin position="22"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK24049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="118" mass="12435" checksum="63C78629B0998E30" modified="2012-06-13" version="1">MATQSFRFLLLPLLGPLLLLGAELTTVCHGRVTPVFAVEAATTAMAHDADSPPSNGHSSFHGSDHQEGAMASSSSSRKLAKVRGVVQFSSGHAMLRRHDGVSAESKRVVPQGPNPLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HDF5</accession>
    <accession>A0A0Q3MG03</accession>
    <name>I1HDF5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="9">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100832029</name>
        <name evidence="10" type="ORF">BRADI_2g07407v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>indole-3-pyruvate + NADPH + O2 + H(+) = (indol-3-yl)acetate + CO2 + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:30854"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.168"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03366.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565511.1">
        <property type="nucleotide sequence ID" value="XM_003565463.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HDF5"/>
    <dbReference type="STRING" id="15368.I1HDF5"/>
    <dbReference type="EnsemblPlants" id="KQK03366">
        <property type="protein sequence ID" value="KQK03366"/>
        <property type="gene ID" value="BRADI_2g07407v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832029"/>
    <dbReference type="Gramene" id="KQK03366">
        <property type="protein sequence ID" value="KQK03366"/>
        <property type="gene ID" value="BRADI_2g07407v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832029"/>
    <dbReference type="eggNOG" id="KOG1399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006909_2_0_1"/>
    <dbReference type="OMA" id="GQIMHSS"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103075">
        <property type="term" value="F:indole-3-pyruvate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009901">
        <property type="term" value="P:anther dehiscence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009851">
        <property type="term" value="P:auxin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000100">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF88">
        <property type="entry name" value="INDOLE-3-PYRUVATE MONOOXYGENASE YUCCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK03366.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="446" mass="49594" checksum="D9CCCC52392A0ED4" modified="2012-06-13" version="1">MDCFAEAEGKRSHDPLYCNPPAALATGFPIEDVKFEKVAGPLIVGAGPAGLATAALLGQQNVPYVLLERCSCIGSLWRHRTYDRLCLHLPKNFCELPLMPFPSSFPTYPTREQFLDYLESYAKRFDVQPVFRQAVVSAEFNGEFWWVRTKQVISAAIGGQQAVLGSAITMYRCKWLVVATGENAEPRVPEIEGAKRFKGQIMHSSEYRSGEGYAGKRVLVVGCGNSGMEVSLDLSNHNAHAAMVVRDTVNVLPREILGHSTFGISAWLLKWLPIKTVDMILLTMSRFVMGDITRLGIARPSLGPMELKSVSGKTPVLDVGTIAKIWSGNIQVFPAIQCFKEHGVEFIDGRIKNFDVVILATGYKSNVPYWLKEKQFFSEKDGFPRNSKEWKGKNGLYAVGFSRRGLTGVAMDATQISDDITKNWRDMHTRDVREDPSSRSGTVIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HEA3</accession>
    <name>I1HEA3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PQ-loop repeat family protein / transmembrane family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_2g09940v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03810.1"/>
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    <dbReference type="STRING" id="15368.I1HEA3"/>
    <dbReference type="EnsemblPlants" id="KQK03810">
        <property type="protein sequence ID" value="KQK03810"/>
        <property type="gene ID" value="BRADI_2g09940v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03810">
        <property type="protein sequence ID" value="KQK03810"/>
        <property type="gene ID" value="BRADI_2g09940v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="PQ-loop repeat family protein / transmembrane family protein"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF44">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
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            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
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        <location>
            <begin position="316"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="133"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK03810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="43465" checksum="E805ED982BB65A50" modified="2012-06-13" version="1">MGIFSGTPPPSCPSSPRCAQWAQTYLKYCLCSTKDGVALGLGLASVISWGVAEVPQIITNYKQKSTEGLSIAFLMTWIVGDLFNLAGCFLEPATLPTQFYMALLYTITTVILTGQTLYYSHIYHHVKATKTRATGKPQKHLRRDASLRDKLLGHRDDEAPRNNSQSGVTIPIPSSPIQVSTEVFRQRHGSISPSSDYYYVSARSLSRSPVPIGGAWLGNNRQTTKTPQTNDQNESLVGEFAPAQSAPATITKNSLSVVPWISVLLGMCVLHILVGTAHKEVSNGIIIPVGRKLLALADDHADSSLRHGSGSEIGSFLGWAMAIIYMGGRLPQIFLNMQRGHAEASSFPSSKIFSYLIYCVNIFILKVRFLSTFIFSLLKKCRSRHFATERTLHLPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HG96</accession>
    <name>I1HG96_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-ketoacyl-CoA synthase</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100837378</name>
        <name evidence="15" type="ORF">BRADI_2g16050v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567862.1">
        <property type="nucleotide sequence ID" value="XM_003567814.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HG96"/>
    <dbReference type="FunCoup" id="I1HG96">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HG96"/>
    <dbReference type="EnsemblPlants" id="KQK04806">
        <property type="protein sequence ID" value="KQK04806"/>
        <property type="gene ID" value="BRADI_2g16050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837378"/>
    <dbReference type="Gramene" id="KQK04806">
        <property type="protein sequence ID" value="KQK04806"/>
        <property type="gene ID" value="BRADI_2g16050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837378"/>
    <dbReference type="eggNOG" id="ENOG502QPKZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013238_2_1_1"/>
    <dbReference type="OMA" id="HHSKLCG"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3-ketoacyl-CoA synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="FAE" evidence="13">
        <location>
            <begin position="98"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="14">
        <location>
            <begin position="402"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR036417-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK04806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="518" mass="57406" checksum="045A6018994D1A52" modified="2012-06-13" version="1">MNGGGGMAAPAAAAATPSHRRLPDFLQSVNLKYVKLGYHYLITHLITLLLLPLMAVIVLEAGRTDPDDLRQLWLHLQYNLVSVLLLSAVLVFGATVYALTRPRPVYLVDFACYKPPPHLKVSFQEFLRHSGLCGFSDDALDFQRKILERSGLSEETYCPEGMHAIPPEPTMANARAEAESVMFGALDNLFSATGVKPKDVGILVVNCSLFNPTPSLSAMIVNRYKLRGNVRSFNLGGMGCSAGVIAIDLARDMLQVHRSTYAVVVSTENITQNWYFGNRKSMLIPNCLFRVGGSAVLLSNRGADRRRAKYSLRHVVRTHKGADDKAFNCVYQEQDAEGKTGVSLSKDLMAIAGGALKTNITTLGPLVLPFSEQLLFFATLVSKKLFNAKVKPYIPDFKLAFEHFCIHAGGRAVIDELEKNLHLSPVHVEASRMTLHRFGNTSSSSIWYELAYMEAKGRVRRGDRIWQIAFGSGFKCNSAVWHALRNVKPAANSPWEDCIDRYPVELVDGFPTHNHKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJ36</accession>
    <name>I1HJ36_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100840215</name>
        <name evidence="11" type="ORF">BRADI_2g24300v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-arogenate from prephenate (L-Asp route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-arogenate from prephenate (L-Glu route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568386.2">
        <property type="nucleotide sequence ID" value="XM_003568338.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJ36"/>
    <dbReference type="STRING" id="15368.I1HJ36"/>
    <dbReference type="EnsemblPlants" id="PNT71187">
        <property type="protein sequence ID" value="PNT71187"/>
        <property type="gene ID" value="BRADI_2g24300v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840215"/>
    <dbReference type="Gramene" id="PNT71187">
        <property type="protein sequence ID" value="PNT71187"/>
        <property type="gene ID" value="BRADI_2g24300v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840215"/>
    <dbReference type="eggNOG" id="KOG0257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_1"/>
    <dbReference type="OMA" id="DMVLACD"/>
    <dbReference type="OrthoDB" id="2414662at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033853">
        <property type="term" value="F:aspartate-prephenate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033854">
        <property type="term" value="F:glutamate-prephenate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009095">
        <property type="term" value="P:aromatic amino acid family biosynthetic process, prephenate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000033">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="89"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT71187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="514" mass="55466" checksum="7B1EC2A7CC74540F" modified="2012-06-13" version="1">MAFSSLSTSSSSSSAFTLPTKPSLSTGSVSFSRASCEWQGKQATGKSRMAVVRAEAVDTSISPRVSALRPSKTMAITDQASALRQAGVPVIGLAAGEPDFDTPAAISEAGMNAVKDGFTRYTPNAGTMELRKAICNKLQEDNGLSYSPDQVLVSNGAKQCITQAVLAVCSPGDEVLIPAPFWVSYPEMARLAGATPVILPTSISDNFLLKPESLASVVNENSRLLILCSPSNPTGSVYPRELLEEIADIVKKHPRLLVLSDEIYEHIIYHPAKHTSFAALPGMWERTLTVNGFSKAFAMTGWRLGYLAAPKHFVSACGKVQSQFTSGASNISQKAGLAALNLGYAGGEAVSTMVKAFQERRDYLVRSFRELPGVKISEPKGAFYLFIDFSSYYGSEVEGFGTIKDSETLCMFLLEKAQVALVPGDAFGDNKGVRISYAAALSTLQAAMEKIKEAMALLSPPLLVECHFHQKCHIRVHHPFFLFFLANLQRICFVSTFLTGIFEQQTSSMFSPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJV5</accession>
    <name>I1HJV5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g26650v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJV5"/>
    <dbReference type="FunCoup" id="I1HJV5">
        <property type="interactions" value="708"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HJV5"/>
    <dbReference type="EnsemblPlants" id="KQK06500">
        <property type="protein sequence ID" value="KQK06500"/>
        <property type="gene ID" value="BRADI_2g26650v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06500">
        <property type="protein sequence ID" value="KQK06500"/>
        <property type="gene ID" value="BRADI_2g26650v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0448">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014646_0_0_1"/>
    <dbReference type="InParanoid" id="I1HJV5"/>
    <dbReference type="OMA" id="HFWEDVQ"/>
    <dbReference type="OrthoDB" id="422720at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900425">
        <property type="term" value="P:negative regulation of defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034051">
        <property type="term" value="P:negative regulation of plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010027">
        <property type="term" value="P:thylakoid membrane organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd09912">
        <property type="entry name" value="DLP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000243">
        <property type="entry name" value="Probable transmembrane GTPase FZO-like, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001052">
        <property type="entry name" value="Probable transmembrane GTPase FZO-like, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051943">
        <property type="entry name" value="TRAFAC_Dynamin-like_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681:SF1">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="361"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK06500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="915" mass="99771" checksum="92A32B53114282CD" modified="2012-06-13" version="1">MFAASTATTRLLLPRPAGPCRGLPFHPLLLPRHRSRRAVDARSAASGGSGAAKQPPRTLFPGGFKRPEIQVPALVLRVGVDEALGSGDTVASAVARGVGIVVLEAGEEGGGRAYEAARALKAAVGDRAYLLIAERVDVASAVGASGVVLADDGIPAIVARSMMMKSNSESIYLPLVARTVQSSDSARSASSSEGADFLIVNTRTDDFSSAISGVGAQNVKIPIFFTLNDSQSEGSYSDTSSKLLQSGASGIVLSLAGIHHLTDNIIERDFSRVDTTDGVPQATYLSASTSEETNNVMVLSREKTKVAGFTKLDEKVMELIAMEKPILNEAVAVIRKAAPMMEEVELLVDAASRLSEPFLLVIVGEFNSGKSTFINALLGRQYLQEGVVPTTNEITLLSYSEVDSESMERCERHPDGQFTCYLSAPILKEMNLVDTPGTNVILQRQQRLTEEYVPRADLILFVLSSDRPLTESEVGFLQYVQQWKKKVVFVLNKLDLYRNSDELEEATAFIKENARKLLNTEHVTLFPVSSRSALEVKLSYSKNNDREHYGKVLLNDPRWRSSKFYDLEHYLLSFLDGSTDNGKERVRLKLETPIGIADRLLTSCQRLVKLEYEKSIDDLTAIKDLVSGANSYAVKIEADSNSWQKQISSLIERAKSRAITLMESTLQLSNIDLISTYMLAGEKGTSAKATSFVQNDILSPALDDAVNLMGEYSTWLSSSNIREANLYLECFHERWSSLLTQEERLPSDPNELVNEGEKLSIKALNGFSAYAAAKVFEAEIREVATGTFGGLGVAGLSASLLTSVLTTTLEDLLALALCSAGGFFAISNFPGRRKLALEKVSKAADDLSRKVDEAIQEDISQSASKLVQFVDVLSKPYQDACQRKIDWLQGVQGEMSAVERKLQTFKVDIQNLHGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HK66</accession>
    <name>I1HK66_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phytochrome A-associated F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106866213</name>
        <name evidence="2" type="ORF">BRADI_2g27810v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_014899155.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HK66"/>
    <dbReference type="STRING" id="15368.I1HK66"/>
    <dbReference type="EnsemblPlants" id="KQK06678">
        <property type="protein sequence ID" value="KQK06678"/>
        <property type="gene ID" value="BRADI_2g27810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106866213"/>
    <dbReference type="Gramene" id="KQK06678">
        <property type="protein sequence ID" value="KQK06678"/>
        <property type="gene ID" value="BRADI_2g27810v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ62">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049819_0_0_1"/>
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    <dbReference type="OrthoDB" id="730977at2759"/>
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        <property type="component" value="Chromosome 2"/>
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        <location>
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            <end position="175"/>
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        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="40093" checksum="A78B202654BE14A4" modified="2012-06-13" version="1">MSGAEHKEIVKRRRDDKEEEAAAAPAGAVEALADDVLLQILGRLEADPRDWARASCASPRLAALLRAACFPPRLSRALPAELLPAPAADGAPAAWAALHKMSVCCPGLLRAGILLEPSDDFGLELDIGPDLSVPSASLDATPISSAKPPPPKSAPAESSSSHGKSRTSAELMTPSDPPAAWSLYDDLYLDAAYDCSSSSEAAAQIPAAAEEPTEPAAANAVRARCQGVASGSRGRRARRWLGPVGSHLASGSWTLSREQGNKLLASRFRGDRLYICDWPGCVHAEERRKYMVFRGVFQDFARSQVRRALRDTRRPTVAVACAFCACTEAWDLYSAFCLRSFYGYHDDGEPVVRAYVCENGHVAGAWTERPLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1HMH6</accession>
    <name>I1HMH6_BRADI</name>
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        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100845584</name>
        <name evidence="12" type="ORF">BRADI_2g37790v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003566601.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HMH6"/>
    <dbReference type="STRING" id="15368.I1HMH6"/>
    <dbReference type="EnsemblPlants" id="KQK07814">
        <property type="protein sequence ID" value="KQK07814"/>
        <property type="gene ID" value="BRADI_2g37790v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845584"/>
    <dbReference type="Gramene" id="KQK07814">
        <property type="protein sequence ID" value="KQK07814"/>
        <property type="gene ID" value="BRADI_2g37790v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845584"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045458_1_0_1"/>
    <dbReference type="OMA" id="KVFRDSW"/>
    <dbReference type="OrthoDB" id="427030at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HMH6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:5S rDNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 574"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
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    </dbReference>
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        <property type="entry name" value="Zinc_finger_trans_reg"/>
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    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="105"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="134"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="171"/>
            <end position="200"/>
        </location>
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        <location>
            <begin position="261"/>
            <end position="291"/>
        </location>
    </feature>
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        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK07814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="372" mass="42560" checksum="8E5C2207DCB83B17" modified="2012-06-13" version="1">MGERDFDGGDAGARETEPEAAAPVRDIRRYKCEFCDVVRSKKQLIRAHVLEHHKDEVDDLEEYNEAAGGVQRKEITHDCEECGARFKKPAHLKQHMQSHSSERPFACHVDGCPFSYSRKDHLNRHLLTHQGKLFICPMEGCNRKFSTRGNMQRHVQEMHKDGSPCESKKEFICPEVNCGKAFKFASKLQKHEDSHAKLEYTEVICCEPGCMKTFTNVECLKAHNQSCHRYVQCDVCDTKQLKKNFKRHQRMHEGSRVAETVKCHFEDCKCSFSKKSNLDKHIKAVHEQSRPYACGFAGCGKKFSYKHVRDNHEKSSAHVYFEGDFVESGPHPGGRKRKPIPVDTFTRKRVAAPGDVPTYADGTGYLRWLLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1HP91</accession>
    <name>I1HP91_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100843054</name>
        <name evidence="3" type="ORF">BRADI_2g43040v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569323.1">
        <property type="nucleotide sequence ID" value="XM_003569275.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HP91"/>
    <dbReference type="FunCoup" id="I1HP91">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HP91"/>
    <dbReference type="EnsemblPlants" id="KQK08648">
        <property type="protein sequence ID" value="KQK08648"/>
        <property type="gene ID" value="BRADI_2g43040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843054"/>
    <dbReference type="Gramene" id="KQK08648">
        <property type="protein sequence ID" value="KQK08648"/>
        <property type="gene ID" value="BRADI_2g43040v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843054"/>
    <dbReference type="eggNOG" id="KOG0714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_11_2_1"/>
    <dbReference type="OMA" id="FESPQWF"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44743:SF10">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44743">
        <property type="entry name" value="PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="10"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK08648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="200" mass="21684" checksum="8FAA3701B517E052" modified="2012-06-13" version="1">MQSAESHYQSYYAVLGVHPGASAAEIRAAYHRLAMRWHPDKIANGRADPALAEEAKGRFQKIHEAYQVLSDEKRRALYDAGVYDPLEDGQEEVEGFHDFLQEMLSLMATVGREEPVYSLGELQSMLDGMMQDFASPQPAAAAAAPPSSSSSSSSSFFTGAGSSRFNAPSSGQKRRRSSSRVRPQGFGSSACFSRTAFSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSZ3</accession>
    <name>I1HSZ3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC104582894</name>
        <name evidence="2" type="ORF">BRADI_2g53770v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232503.1">
        <property type="nucleotide sequence ID" value="XM_010234201.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSZ3"/>
    <dbReference type="STRING" id="15368.I1HSZ3"/>
    <dbReference type="EnsemblPlants" id="KQK10386">
        <property type="protein sequence ID" value="KQK10386"/>
        <property type="gene ID" value="BRADI_2g53770v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104582894"/>
    <dbReference type="Gramene" id="KQK10386">
        <property type="protein sequence ID" value="KQK10386"/>
        <property type="gene ID" value="BRADI_2g53770v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104582894"/>
    <dbReference type="eggNOG" id="ENOG502S11E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094736_0_0_1"/>
    <dbReference type="OMA" id="HVKHVSG"/>
    <dbReference type="OrthoDB" id="1856818at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33052">
        <property type="entry name" value="DUF4228 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK10386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="166" mass="17890" checksum="1AABFA2B5376FE30" modified="2012-06-13" version="1">MGNSLRCCLTCMLPCGSLDVVRIVHLSGRVDEFGCPITGAAVLAAHPNHTLATAWSSAGVGCPTKKLVIVSPDSELKRGRIYFLIPSATVPGADRRKKNRPPCSKSKQSSKRTTRASSTAEQDNYLTELLLSEKAASAGAHRRRRSGCRIGVWRPELESIVEETSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUN9</accession>
    <name>I1HUN9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100823043</name>
        <name evidence="7" type="ORF">BRADI_2g59047v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564949.2">
        <property type="nucleotide sequence ID" value="XM_003564901.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUN9"/>
    <dbReference type="FunCoup" id="I1HUN9">
        <property type="interactions" value="864"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HUN9"/>
    <dbReference type="EnsemblPlants" id="KQK11258">
        <property type="protein sequence ID" value="KQK11258"/>
        <property type="gene ID" value="BRADI_2g59047v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823043"/>
    <dbReference type="Gramene" id="KQK11258">
        <property type="protein sequence ID" value="KQK11258"/>
        <property type="gene ID" value="BRADI_2g59047v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823043"/>
    <dbReference type="eggNOG" id="KOG1021">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033763_1_1_1"/>
    <dbReference type="OMA" id="EKWLEMW"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF48">
        <property type="entry name" value="OJ1485_B09.5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Exostosin GT47" evidence="6">
        <location>
            <begin position="56"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK11258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="433" mass="48541" checksum="D8DE9D7CBA0A7C0C" modified="2012-06-13" version="1">MMAAPSSRAVAIGGSLLLLLVFALSSTVIYLTSSGPAASPSSILLNLRPAKCPPAPPLRVFMYDLPARFHVAMMTTAANGSGGEGFPAWPPSAGGIRRQHSVEYWMMASLQGGGGEGKFGAREAVRVADPEAAEAFFVPFFSSLSFNVHGRNMTDPDTEADRLLQVELIDVLWKSKYWQRSAGRDHVIPMHHPNAFRFLRDMVNASVLIVADFGRYTQELASLRKDVVAPYVHVVDSFINDDPPDPFEARPTLLFFRGRTVRKAEGKIRAKLAKILKDKDGVRFEDSLATGEGINTSTEGMRSSKFCLHPAGDTPSSCRLFDAIVSHCIPVIVSSRIELPFEDEIDYSEFSLFFSVEEALKPDYLLDQLRQMPKEKWVEMWSKLKNVSSHYEFQYPTRKGDAVNMIWRQVRHKIPAVNLAIHRNRRLKIPDWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HVY8</accession>
    <name>I1HVY8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SKP1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100843377</name>
        <name evidence="11" type="ORF">BRADI_3g00340v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends on the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, a RING-box and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends on the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of a SCF (SKP1-cullin-F-box) protein ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573486.1">
        <property type="nucleotide sequence ID" value="XM_003573438.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HVY8"/>
    <dbReference type="STRING" id="15368.I1HVY8"/>
    <dbReference type="EnsemblPlants" id="KQJ92734">
        <property type="protein sequence ID" value="KQJ92734"/>
        <property type="gene ID" value="BRADI_3g00340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843377"/>
    <dbReference type="Gramene" id="KQJ92734">
        <property type="protein sequence ID" value="KQJ92734"/>
        <property type="gene ID" value="BRADI_3g00340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843377"/>
    <dbReference type="eggNOG" id="KOG1724">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059252_6_1_1"/>
    <dbReference type="OMA" id="IAIPGCK"/>
    <dbReference type="OrthoDB" id="1903179at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009867">
        <property type="term" value="P:jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000057">
        <property type="entry name" value="SKP1-like protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="10">
        <location>
            <begin position="8"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="9">
        <location>
            <begin position="119"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ92734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="168" mass="18709" checksum="5C2D632FC244DC45" modified="2012-06-13" version="1">MASDGEKKMITLKSSDGEEFEVEETVAMESQTIRHMIEDDCADNGIPLPNVNSKILSKVIEYCNKHVHAADATDAAAANTSAAPAAPTDDLKNWDADFVKVDQATLFDLILAANYLNIKGLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEIRRENQWAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HY37</accession>
    <name>I1HY37_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glycerophosphodiester phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100827169</name>
        <name evidence="10" type="ORF">BRADI_3g06402v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a sn-glycero-3-phosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:83408"/>
            <dbReference type="EC" id="3.1.4.46"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.4.46" evidence="1"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570935.1">
        <property type="nucleotide sequence ID" value="XM_003570887.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233932.1">
        <property type="nucleotide sequence ID" value="XM_010235630.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HY37"/>
    <dbReference type="STRING" id="15368.I1HY37"/>
    <dbReference type="EnsemblPlants" id="KQJ93736">
        <property type="protein sequence ID" value="KQJ93736"/>
        <property type="gene ID" value="BRADI_3g06402v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827169"/>
    <dbReference type="Gramene" id="KQJ93736">
        <property type="protein sequence ID" value="KQJ93736"/>
        <property type="gene ID" value="BRADI_3g06402v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827169"/>
    <dbReference type="eggNOG" id="KOG2258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010414_0_0_1"/>
    <dbReference type="OMA" id="GFDYWAD"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08603">
        <property type="entry name" value="GDPD_SHV3_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08604">
        <property type="entry name" value="GDPD_SHV3_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000011">
        <property type="entry name" value="Glycerophosphodiester phosphodiesterase GDPDL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000013">
        <property type="entry name" value="Glycerophosphodiester phosphodiesterase GDPDL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620:SF44">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE GDPDL6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094899" description="glycerophosphodiester phosphodiesterase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="37"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="355"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="704"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="704"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ93736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="750" mass="81425" checksum="57195131250F9290" modified="2012-06-13" version="1">MGGRYPHMLLILLFLHGTNAALDAPVPKWQTLEGRPPLVIAHAGFSGLFPDSSQLAYQVAMATSLHDVILHCDLQLSSDAIGFCKTGLRLDKSTLIAEIFPKRDKTYKVGAEDVHGWFSVDFTADELSHNVTLMQSIFSRPSTFDGSMGMYTLDDVAELRPQQIWVNVEFDSFFKDHKLSTEDYLLGLPKEYPITYISSPDISFLESISGKLKGKTKIILRCLYENVTETTVKKKYGEIVKDLKSIKAFASGIMVPRNYIWPLNHEQYLLPPTSLVKDAHALGLEVYAAGFANDVFTSYNYSYDPATEYLQFIDNSDFCVDGVLTDFPPTASGAIACLAHTKGNVLRPTDDGERPFIITHNGASGVFPGCTDLAYQQAVKDGADIIDCPVRMSKDGVAFCLASADLTSSTTAATTFMTKVVTINEIQNKSGIFSFDLSWSEIQSVKPDLIGPYAPVGLKRNPAVKNAGKFLTLTEFLDFAKTSNVSGIMIEIEHASYLATRGLGVVDAVTKSLVNASYDKEDKQHVLIQSDDSSVLSAFKNFPTFQRVLVVHSVISDASKPSIDEIKEFAHAVSVTRTSLVEVNGFFLTGFTNLVERLHAANISVYAGVLKNEFMNLGFDYWADPMMEIATYSASLAVDGLVTEFPATAIAYFKSPCSDLTLNLSYTILPAEPGSLVSQVPPGALPPALPPAPVLEPADVLDPPLPPVSVSSPPEATPKAADDSASAASSNVSNYLLVVGIAAFLSLSFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYD6</accession>
    <name>I1HYD6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Signal recognition particle subunit SRP68</fullName>
            <shortName evidence="12">SRP68</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100830023</name>
        <name evidence="14" type="ORF">BRADI_3g07210v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). The SRP complex interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the SRP68 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571269.1">
        <property type="nucleotide sequence ID" value="XM_003571221.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYD6"/>
    <dbReference type="FunCoup" id="I1HYD6">
        <property type="interactions" value="3154"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HYD6"/>
    <dbReference type="EnsemblPlants" id="KQJ93876">
        <property type="protein sequence ID" value="KQJ93876"/>
        <property type="gene ID" value="BRADI_3g07210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830023"/>
    <dbReference type="Gramene" id="KQJ93876">
        <property type="protein sequence ID" value="KQJ93876"/>
        <property type="gene ID" value="BRADI_3g07210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830023"/>
    <dbReference type="eggNOG" id="KOG2460">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018649_1_0_1"/>
    <dbReference type="OMA" id="DERFIHI"/>
    <dbReference type="OrthoDB" id="10255118at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15481">
        <property type="entry name" value="SRP68-RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3450.40:FF:000001">
        <property type="entry name" value="Signal recognition particle subunit SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.40">
        <property type="entry name" value="Signal recognition particle, SRP68 subunit, RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026258">
        <property type="entry name" value="SRP68"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034652">
        <property type="entry name" value="SRP68-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038253">
        <property type="entry name" value="SRP68_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 68 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16969">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038995">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8 12" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ93876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="603" mass="68398" checksum="6214C112EDD4CBB0" modified="2012-06-13" version="1">MSKLANQSPPPPQQPSDMEVDSVAAAEEKSTVRFSINVLELMREAQMQHGLRHGDYTRYRRYCTARLRRLYKSLKFLHGRGKYTRRSITESTVTDVRFLHVVFYMSERAWSHAMEKKTAGPSARQRIYMLGRFRKAVKWATLFSQLCSIKGDSRTSLEAEAYASYMKGTLLFEQEKNIEAAMMNFKNTRTVYEELGKYGSIENQLLCRQRIEEVEPMIGFCSRKLGGSALQEHELLDMEKEGPAYDLFKAKIEAVLSETRSQQAASMTEFNWLGRRFPISSAKTRVSILKAQQLERDLNGAAKESIPADKKLAIFDKLFSAYHDARGCIRNDLASAGNAENIRDDLNGLDKAVSAVLGLRTIERNQLLVSIAKSKFTKHQDEKNEKITKPEELVRLYDLLIQNTTDLTDLVSSGRDKNKEENTFLQEYELKGLAFRAERCFYLAKSYSSAGKRAEAYALFCYARSLTDSALQQLANSSDKALIQDLKALSDNCRSNSCIEHATGIMENESAPEKLSKGVSTMSLGDDKRKENKYLLEMLESYESALGEPNTKATSRIAQFPPPFQSVPCNPIVLDMAYNAIDFPNLENRMKKEKKGLLSRLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYS9</accession>
    <name>I1HYS9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cytokinin dehydrogenase</fullName>
            <ecNumber evidence="4">1.5.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100839711</name>
        <name evidence="11" type="ORF">BRADI_3g08230v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-dimethylallyladenine + A + H2O = 3-methyl-2-butenal + adenine + AH2</text>
            <dbReference type="Rhea" id="RHEA:13625"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15825"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="EC" id="1.5.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.99.12" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571719.1">
        <property type="nucleotide sequence ID" value="XM_003571671.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYS9"/>
    <dbReference type="STRING" id="15368.I1HYS9"/>
    <dbReference type="EnsemblPlants" id="KQJ94054">
        <property type="protein sequence ID" value="KQJ94054"/>
        <property type="gene ID" value="BRADI_3g08230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839711"/>
    <dbReference type="Gramene" id="KQJ94054">
        <property type="protein sequence ID" value="KQJ94054"/>
        <property type="gene ID" value="BRADI_3g08230v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839711"/>
    <dbReference type="eggNOG" id="KOG1231">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024955_1_0_1"/>
    <dbReference type="OMA" id="TAKWNSH"/>
    <dbReference type="OrthoDB" id="415825at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019139">
        <property type="term" value="F:cytokinin dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009690">
        <property type="term" value="P:cytokinin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.10">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016170">
        <property type="entry name" value="Cytok_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015345">
        <property type="entry name" value="Cytokinin_DH_FAD/cytokin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050432">
        <property type="entry name" value="FAD-linked_Oxidoreductases_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006093">
        <property type="entry name" value="Oxy_OxRdtase_FAD_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878:SF127">
        <property type="entry name" value="CYTOKININ DEHYDROGENASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878">
        <property type="entry name" value="GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09265">
        <property type="entry name" value="Cytokin-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00862">
        <property type="entry name" value="OX2_COVAL_FAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094900" description="cytokinin dehydrogenase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="10">
        <location>
            <begin position="66"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ94054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="532" mass="57773" checksum="799AC2B47E748A0D" modified="2012-06-13" version="1">MAARARLVAFLIGMASFLSIVAAGQIRPLPSGGQLPSGLFVHSIASMIRTDRNATTKASSDFGRIVEAAPEAVLHPATPADIAALIRFSASSKAPFPVAPRGQGHSARGQSLAPGGVVVDMRALGRGHRRINVSAGAGYVDAGGEQLWIDVLRATLEHGLAPRAWTDYLRLTVGGTLSNAGIGGQAFRHGPQIANVRELDVVTGTGELVTCSREESSDLFFAVLGGLGQFGVITRARIALEPAPKRARWVRLAYTDVTVFTGDQELLISKKASEAGFDYVEGQVQLNRTLIDGPESTPFFSGADINRLAGLVSRSGSGAIYFIEAAMYYDEATASSVDQKLEAVLEQLSFTPGFVFTKDVTYVQFLDRVRVEEEVLRSVGVWDVPHPWLNLFIPRSRIIGFDTGVLKGILGGTNPVGVILMYPMNTNKWDDRMTAVTPQTEEDVFYAVSLLRSAVAVGDVERLERENEAVLAFCTREAIRCKQYLPHYTSEDGWRRHFGTKWERIAELKAKYDPQTIMAPGQRIFQSSIASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6I0</accession>
    <name>I1I6I0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100840138</name>
        <name evidence="14" type="ORF">BRADI_3g34410v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class IV subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235052.1">
        <property type="nucleotide sequence ID" value="XM_010236750.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318297.1">
        <property type="nucleotide sequence ID" value="XM_024462529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6I0"/>
    <dbReference type="FunCoup" id="I1I6I0">
        <property type="interactions" value="1163"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I6I0"/>
    <dbReference type="EnsemblPlants" id="KQJ97974">
        <property type="protein sequence ID" value="KQJ97974"/>
        <property type="gene ID" value="BRADI_3g34410v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67971">
        <property type="protein sequence ID" value="PNT67971"/>
        <property type="gene ID" value="BRADI_3g34410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840138"/>
    <dbReference type="Gramene" id="KQJ97974">
        <property type="protein sequence ID" value="KQJ97974"/>
        <property type="gene ID" value="BRADI_3g34410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67971">
        <property type="protein sequence ID" value="PNT67971"/>
        <property type="gene ID" value="BRADI_3g34410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840138"/>
    <dbReference type="eggNOG" id="ENOG502QU3P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015002_2_1_1"/>
    <dbReference type="OMA" id="QAPVMGC"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08875">
        <property type="entry name" value="START_ArGLABRA2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000229">
        <property type="entry name" value="Homeobox-leucine zipper protein HDG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042160">
        <property type="entry name" value="HD-Zip_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057993">
        <property type="entry name" value="HD-Zip_IV_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45654:SF11">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HDG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45654">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN MERISTEM L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25797">
        <property type="entry name" value="PDF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="123"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="13">
        <location>
            <begin position="349"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="125"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="805"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="805"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="PNT67971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="888" mass="95761" checksum="E56449DC0D9170C0" modified="2012-06-13" version="1">MFGDCQVLSSMAAMAGGSSSADALFASPLMQPNPGGFNMGMAPFHHHHFSTTLIPKEEGVMSMGKDDDMDLTEMDMAELSGGSGSAHLDGLLAFDDDDHKPHHNEPNMASTGAANNGNNAANGGKKKRYHRHTAHQIQQMEALFKECPHPDDKQRLKLSQELGLKPRQVKFWFQNRRTQMKAQQDRADNVILRAENESLKTDNFRLQAALRNVVCPTCGHPGAVLADVPYEEQTLRVENARLKDELDRLASIATRYGGGRQQQQPGGGMSSLLGCMSAMSAPMLMPPQQLDLDMSVYSRHFADHQQHGGGAMADHHLMTSVPQLQMADHGHQHPGVSAVSSYMAPVVVQEQDRQLVLDLAATAAEHLARMCRAGDPLWARRVAGASGEVMDAAEHARVFSWPVVDAGNKQQQQGDLAAASAALRTEGTRDGAVVIMNSITLVDAFLDANKWMELFPSIVSKARTIQVIHNGAASGHLASGSLILMQAEVQFLSPLVPAREVVFFRYCVHNADEGSWSIVDFPADGFQDDLLQQQQQTSAVVRCRRRPSGCIIQDAPNGYSRVVWVEHMEVVGDEKPLQPVFKDHVAGGAAFGATRWVAVLQRQCERLASELARNIADQGVIRTPEARTNMMRLSQRMITAFCANISASGSQSWTALSDSTEDTIRVTTRKNTEPGQPSGVILTAVSTSWLPFSHQQVFELLADEQQRCQLEIMSNGSSLQEVAHIANGSHPRNCISLLRINAASNSSQNVELLLQETSTHPDGGSLVVFATVDVDAIQVTMSGEDPSYIPLLPLGFAIFPATNPSPSATRANTGDGESTPGNADEPANGCLLTVGMQVLASAVPSAKLNLSSVTAINSHICNTIHQITTALKGAGASRSEPVSAGSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8B6</accession>
    <name>I1I8B6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Micro-fibrillar-associated protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100838204</name>
        <name evidence="4" type="ORF">BRADI_3g39560v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574719.1">
        <property type="nucleotide sequence ID" value="XM_003574671.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756403.1">
        <property type="nucleotide sequence ID" value="XM_014900917.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8B6"/>
    <dbReference type="STRING" id="15368.I1I8B6"/>
    <dbReference type="EnsemblPlants" id="KQJ98858">
        <property type="protein sequence ID" value="KQJ98858"/>
        <property type="gene ID" value="BRADI_3g39560v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ98859">
        <property type="protein sequence ID" value="KQJ98859"/>
        <property type="gene ID" value="BRADI_3g39560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838204"/>
    <dbReference type="Gramene" id="KQJ98858">
        <property type="protein sequence ID" value="KQJ98858"/>
        <property type="gene ID" value="BRADI_3g39560v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ98859">
        <property type="protein sequence ID" value="KQJ98859"/>
        <property type="gene ID" value="BRADI_3g39560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838204"/>
    <dbReference type="eggNOG" id="KOG1425">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023077_0_1_1"/>
    <dbReference type="OMA" id="FHNERAG"/>
    <dbReference type="OrthoDB" id="782526at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I8B6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033194">
        <property type="entry name" value="MFAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15327">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Micro-fibrillar-associated protein 1 C-terminal" evidence="3">
        <location>
            <begin position="170"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="58"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="414"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ98858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="437" mass="51442" checksum="FE558CB4F5AFFDDD" modified="2012-06-13" version="1">MSVAAGVSDAAIAVRDKLRGKIGQTKVKRYWPGKAPEWGGEDADDDIDIRTARVSLDKAFPKDDDGGRPAKDDRRLRRLAEARGESKEELRADHRRIRQAEIVSTAEEERDRQEAELEEEGDEEAQEERRRRIRARQLLRQQEEELLPQEEEPVEEEQEEDGEESEYETDSEDEQMGMAMVKPVFIPKSQRDTIAERERLEEEERQLEELVKKRLQARKIETRQIVVEEIRKEEQIHKTLNEEASIEDIDTDDELNEAEEYESWKNREIARIKRDREERDARLKEKEEIDKVRNMTEEERREWERKNPKPLRQSKQKWNFMQKYYHKGAFFQEGADDVSQSVGRDDIYTRDFSAPTGEDKMDKSILPKVMQVKHFGRSGRTKWTHLVNEDTTDWNAPWSTNGPLRAKYNAKMAGMNGPIAKPKGSKKMKDWDTKQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1I979</accession>
    <name>I1I979_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RHOMBOID-like protein</fullName>
            <ecNumber evidence="6">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100827999</name>
        <name evidence="8" type="ORF">BRADI_3g42110v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Serine protease involved in intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="6"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572482.1">
        <property type="nucleotide sequence ID" value="XM_003572434.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I979"/>
    <dbReference type="STRING" id="15368.I1I979"/>
    <dbReference type="MEROPS" id="S54.015"/>
    <dbReference type="EnsemblPlants" id="KQJ99263">
        <property type="protein sequence ID" value="KQJ99263"/>
        <property type="gene ID" value="BRADI_3g42110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827999"/>
    <dbReference type="Gramene" id="KQJ99263">
        <property type="protein sequence ID" value="KQJ99263"/>
        <property type="gene ID" value="BRADI_3g42110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827999"/>
    <dbReference type="eggNOG" id="KOG2289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011531_0_0_1"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936:SF85">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="7">
        <location>
            <begin position="152"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ99263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="364" mass="38169" checksum="A11411E212A41541" modified="2012-06-13" version="1">MAIIEVVDGQEIMEAMANGAHGPGVAVAVVMANDIHCTGGAMALTMANGVECPGIIKVGPNGFSIEMLSDPLARFWPMFVPYVICVCVSVFSIVMYVNDCPGNTPGAAASCVAAGFGRVSFQPIHENPLLGPSYATLEKMGALDWAKVVHGHQISRLFTCFWVHAGLIHLFVTQLSLCSFGVRFEQQFGFLRIVIIYLLSGLGGSVLSALFLPAGSVSVGASGPVLGLIGAMVSEIAINWNAYSNRKPALAILGLIAVINMVMGIFPHTDNFTNIGGFLTGFLLGFLVLADPARIGFLPQSRTSEQPKYKSYHYAVFGASLLLLLVGFAVALTVLFEGKNGGGGGGGLFLNCVPTSGWKCSSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJ70</accession>
    <name>I1IJ70_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100827504</name>
        <name evidence="3" type="ORF">BRADI_4g09520v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
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        <property type="protein sequence ID" value="KQJ87164.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="F:GTP binding"/>
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        <property type="term" value="F:GTPase activity"/>
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        <property type="entry name" value="Obg"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="G_OBG"/>
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        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
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        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 2"/>
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        <property type="entry name" value="MMR_HSR1"/>
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        <property type="entry name" value="GTP1OBG"/>
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        <property type="entry name" value="G_OBG"/>
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    <proteinExistence type="predicted"/>
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    <feature type="domain" description="OBG-type G" evidence="2">
        <location>
            <begin position="32"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ87164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="209" mass="23076" checksum="2628BA27F969B38A" modified="2012-06-13" version="1">MSKENRLQEIARLSGGQQGTESFLVLELKSIADVGLVGLPNAGKSTLLSALSRAQPEIADYAFTTLRPNIGSLTYEDYFSVKVADIPGLIKGARENRGLGHAFLRHIERTKVLTYVLDLAATLNGRKGTPPWEQLHDLVVELEHYQEGLTKRPSLMVANNIDEEGADAMYEELKRRVQVVPIFPVCAILRDLMDASDPQGVDMSKVMVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJL0</accession>
    <name>I1IJL0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TCTP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100832998</name>
        <name evidence="5" type="ORF">BRADI_4g10920v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TCTP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003577218.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJL0"/>
    <dbReference type="FunCoup" id="I1IJL0">
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    </dbReference>
    <dbReference type="STRING" id="15368.I1IJL0"/>
    <dbReference type="EnsemblPlants" id="KQJ87403">
        <property type="protein sequence ID" value="KQJ87403"/>
        <property type="gene ID" value="BRADI_4g10920v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832998"/>
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        <property type="protein sequence ID" value="KQJ87403"/>
        <property type="gene ID" value="BRADI_4g10920v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832998"/>
    <dbReference type="eggNOG" id="KOG1727">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095877_1_1_1"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Translationally-controlled tumor protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="Mss4-like_sf"/>
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    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
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        <property type="entry name" value="Translation_control_tumour_CS"/>
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        <property type="entry name" value="Translational_control_tumour_p"/>
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    <dbReference type="PANTHER" id="PTHR11991">
        <property type="entry name" value="TRANSLATIONALLY CONTROLLED TUMOR PROTEIN-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991:SF0">
        <property type="entry name" value="TRANSLATIONALLY-CONTROLLED TUMOR PROTEIN"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TCTP" evidence="4">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ87403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="168" mass="18829" checksum="88D4CB31C519CDDC" modified="2012-06-13" version="1">MLVYQDKLTGDELLSDSFPYKELENGVLWEVDGHWVVQGAVDVDIGANPSAEGGGEDEGVDDQAVKVVDIVDTFRLQEQPAFDKKQFVAYIKRYIKNLTPKLEADEQEVFKKNVEGATKFLLSKIKDLQFFVGESMGDDASVVFAYYKEGATDPTFLYFAHGLKEVKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJQ5</accession>
    <name>I1IJQ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC112268859</name>
        <name evidence="6" type="ORF">BRADI_4g11320v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family. CC-type subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310814.1">
        <property type="nucleotide sequence ID" value="XM_024455046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJQ5"/>
    <dbReference type="STRING" id="15368.I1IJQ5"/>
    <dbReference type="EnsemblPlants" id="KQJ87464">
        <property type="protein sequence ID" value="KQJ87464"/>
        <property type="gene ID" value="BRADI_4g11320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112268859"/>
    <dbReference type="Gramene" id="KQJ87464">
        <property type="protein sequence ID" value="KQJ87464"/>
        <property type="gene ID" value="BRADI_4g11320v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_6_0_1"/>
    <dbReference type="OMA" id="MAMEHVA"/>
    <dbReference type="OrthoDB" id="620792at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011905">
        <property type="entry name" value="GlrX-like_pln_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02189">
        <property type="entry name" value="GlrX-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10168">
        <property type="entry name" value="GLUTAREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="5">
        <location>
            <begin position="13"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ87464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="105" mass="11213" checksum="3D3DCF7308C78CCF" modified="2012-06-13" version="1">MDQVATLASERAVVVFTTSRCSMCPVVTSLLRELGVNAAVHELDKDPQGREMERELARRLGRGSTSVVPAVFIGGNLVGGTNRVMALHLAGQLVPMLKNAGALWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IKL5</accession>
    <name>I1IKL5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100844508</name>
        <name evidence="14" type="ORF">BRADI_4g14490v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577389.1">
        <property type="nucleotide sequence ID" value="XM_003577341.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IKL5"/>
    <dbReference type="FunCoup" id="I1IKL5">
        <property type="interactions" value="1448"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IKL5"/>
    <dbReference type="EnsemblPlants" id="KQJ87941">
        <property type="protein sequence ID" value="KQJ87941"/>
        <property type="gene ID" value="BRADI_4g14490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844508"/>
    <dbReference type="Gramene" id="KQJ87941">
        <property type="protein sequence ID" value="KQJ87941"/>
        <property type="gene ID" value="BRADI_4g14490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844508"/>
    <dbReference type="eggNOG" id="KOG0335">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_16_3_1"/>
    <dbReference type="OMA" id="SYAGMQP"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17967">
        <property type="entry name" value="DEADc_DDX3_DDX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000397">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044763">
        <property type="entry name" value="Ded1/Dbp1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="172"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="385"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="539"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="541"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="572"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ87941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="609" mass="64895" checksum="CFD4C935151AD913" modified="2012-06-13" version="1">MAAPSMTSWADVADAEPAPAPAPASDGPARSDRSSYVPPHLRNRSSAAPPASSAPPPRAAPGLLGRPAPGVPGRFVGGGGGGAAPPPRRWDREPNPFGNDEPVPAAEPEGFDEHQNTGINFDAYEDIPVETSGREVPPAVSTFAEIDLGAALNDNIRRCKYVRPTPVQRHAIPISLAGRDLMACAQTGSGKTAAFCFPIISGIMRGPPVQRPQRGGSRTACPLALILSPTRELSMQIHEEARKFSYQTGVRVVVAYGGAPITQQLRDLERGVDILVATPGRLVDLLERARVSLQSIRYLALDEADRMLDMGFEPQVRRIVEQMDMPPRGVRQTLLFSATFPGEIQRMASDFLENYIFLAVGRVGSSTELIAQRVEFVHEADKRSHLMDLLHAQRDSADHGKQALTLVFVETKRGADSLENWLCTNGFPATSIHGDRNQQEREYALRSFKSGQTPILVATDVAARGLDIPHVAHVVNFDLPNDIDDYVHRIGRTGRAGKSGLATAFFSDNNASMAKSLADLMQESNQEVPAWLTRYAARPSYGGGGGGRNRRGGGGGSRFGGRDFRRESSSFGKGGDYGGGSNGGAGSYGGSSSYGGGGYGGAGAPSAWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPJ8</accession>
    <name>I1IPJ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100832380</name>
        <name evidence="12" type="ORF">BRADI_4g28627v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238085.1">
        <property type="nucleotide sequence ID" value="XM_010239783.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPJ8"/>
    <dbReference type="FunCoup" id="I1IPJ8">
        <property type="interactions" value="1862"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89945">
        <property type="protein sequence ID" value="KQJ89945"/>
        <property type="gene ID" value="BRADI_4g28627v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832380"/>
    <dbReference type="Gramene" id="KQJ89945">
        <property type="protein sequence ID" value="KQJ89945"/>
        <property type="gene ID" value="BRADI_4g28627v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_21_1_1"/>
    <dbReference type="OMA" id="TSTHCEI"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000258">
        <property type="entry name" value="Probable serine/threonine-protein kinase PBL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000228">
        <property type="entry name" value="Serine/threonine-protein kinase BIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="141"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="63"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="447"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="448"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ89945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="495" mass="54589" checksum="12FE39DF48F2BF24" modified="2012-06-13" version="1">MGLAPPELGQFDGWESSGEEERERWGWCRRNRSNRRRLPRKGSEDASVTTGCCIRLWPMGSCPPPPRSKGDTSTSSASTHGEKSTENGSRNQPVASVVSGSTSTSNAESSSSASKAGEEIKVSSQLRKFAFNDLKCATRNFRPESLLGEGGFGCVFKGWIEENGTAPVKPGTGLTVAVKTLNHDGLQGHKEWVAEVDFLGNLHHPNLVRLIGYCVEDDQRLLVYEFMPRGSLDNHLFRRSLPLPWSIRMKVALGAAQGLSFLHEEAERPVIYRDFKTSNILLDADYNAKLSDFGLAKDGPVGDKTHVSTRVMGTYGYAAPEYVMTGHLTSKSDVYSFGVVLLEMMSGRRSMDKNRPNGEHNLVEWARPLLGERQRFYKLVDPRLEGNFSVKGAQKAAQLARACLSRDPKARPLMSQVVEALKPLLNLKDMASSSYFYQTMQAERMAHSSSLNGRSSHSAKAHGSFARASGQQPVRSISDGPRASPFRYSPKPNMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQ50</accession>
    <name>I1IQ50_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100837931</name>
        <name evidence="12" type="ORF">BRADI_4g30290v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578119.1">
        <property type="nucleotide sequence ID" value="XM_003578071.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQ50"/>
    <dbReference type="FunCoup" id="I1IQ50">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ90241">
        <property type="protein sequence ID" value="KQJ90241"/>
        <property type="gene ID" value="BRADI_4g30290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837931"/>
    <dbReference type="Gramene" id="KQJ90241">
        <property type="protein sequence ID" value="KQJ90241"/>
        <property type="gene ID" value="BRADI_4g30290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837931"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="OMA" id="FMQTFAP"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015145">
        <property type="term" value="F:monosaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17361">
        <property type="entry name" value="MFS_STP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000002">
        <property type="entry name" value="Sugar transport protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044778">
        <property type="entry name" value="MFS_STP/MST-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF595">
        <property type="entry name" value="OS09G0416200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="30"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ90241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="521" mass="56458" checksum="9F7FB93BF3FFF1DD" modified="2012-06-13" version="1">MAGGGVGALGVKKERAAEYKGRMTWAVAMACLVAAVGGAIFGYDIGISGGVTSMDPFLEKFFPVVFRRKNSGHQNNYCKYDNQGLSAFTSSLYLAGLVSSLVASPVTRNYGRRASIVCGGVSFLIGAVLNVAAVNLAMLILGRIMLGVGIGFGNQGVPLYLSEMAPAHLRGGLNMMFQLATTLGIFTANMINYGTQNLKPWGWRLSLGLAAAPALLMTVGGLLLPETPNSLIERGRAQEGRRVLERIRGTADVDAEFTDMAEASELANTIEHPFRNILEPRNRPQLVMAVCMPAFQILTGINSILFYAPVLFQTMGFGADASLYSSVITGAVLFFSTLISIATVDRLGRRKLLISGGIQMIVCQVIVAVILGVKFGTDKQLTRSYSVAVVVVICLFVMAFGWSWGPLGWTVPSEIFPLETRSAGQSITVAVNLFFTFVIAQAFLSLLCAFKFGIFLFFAGWIAVMTVFVYVFLPETKGVPIEEMVLLWRKHWFWKKVMPDMPLHEEDGWGGAGDGARAHHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQX2</accession>
    <name>I1IQX2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100830982</name>
        <name evidence="6" type="ORF">BRADI_4g32680v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578265.1">
        <property type="nucleotide sequence ID" value="XM_003578217.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQX2"/>
    <dbReference type="FunCoup" id="I1IQX2">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IQX2"/>
    <dbReference type="EnsemblPlants" id="KQJ90590">
        <property type="protein sequence ID" value="KQJ90590"/>
        <property type="gene ID" value="BRADI_4g32680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830982"/>
    <dbReference type="Gramene" id="KQJ90590">
        <property type="protein sequence ID" value="KQJ90590"/>
        <property type="gene ID" value="BRADI_4g32680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830982"/>
    <dbReference type="eggNOG" id="ENOG502RZ8U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058719_3_2_1"/>
    <dbReference type="OMA" id="NTPGTHY"/>
    <dbReference type="OrthoDB" id="206968at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000003">
        <property type="entry name" value="Blue copper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028871">
        <property type="entry name" value="BlueCu_1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF439">
        <property type="entry name" value="OS09G0469300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00196">
        <property type="entry name" value="COPPER_BLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095479" description="Phytocyanin domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="5">
        <location>
            <begin position="23"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ90590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="166" mass="15848" checksum="AD0203F969397EFC" modified="2012-06-13" version="1">MASSSVALVALLVASCAGMAAAASFTVGDAQGWVAGIDYSGWTSGKSFAVGDTLVFTYASKVHTVTEVSKSGYAACSGSSALGNDDSGSTTVTLSTPGTHYYICNIPGHCASGMKLAVNVGGGGGGSSSGSGAGIPSGAAAVRVPAMSAAIVLAAASGVLIKAALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITK4</accession>
    <name>I1ITK4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutathione transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100832817</name>
        <name evidence="7" type="ORF">BRADI_4g40170v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Zeta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578780.1">
        <property type="nucleotide sequence ID" value="XM_003578732.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITK4"/>
    <dbReference type="STRING" id="15368.I1ITK4"/>
    <dbReference type="EnsemblPlants" id="KQJ91858">
        <property type="protein sequence ID" value="KQJ91858"/>
        <property type="gene ID" value="BRADI_4g40170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832817"/>
    <dbReference type="Gramene" id="KQJ91858">
        <property type="protein sequence ID" value="KQJ91858"/>
        <property type="gene ID" value="BRADI_4g40170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832817"/>
    <dbReference type="eggNOG" id="KOG0868">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_20_1_1"/>
    <dbReference type="OMA" id="RMDCDLS"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ITK4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016034">
        <property type="term" value="F:maleylacetoacetate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03191">
        <property type="entry name" value="GST_C_Zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03042">
        <property type="entry name" value="GST_N_Zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000100">
        <property type="entry name" value="Glutathione S-transferase Z1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000017">
        <property type="entry name" value="Maleylacetoacetate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005955">
        <property type="entry name" value="GST_Zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034330">
        <property type="entry name" value="GST_Zeta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034333">
        <property type="entry name" value="GST_Zeta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01262">
        <property type="entry name" value="maiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673:SF5">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="86"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ91858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="212" mass="23641" checksum="307DBAF32FEA2687" modified="2012-06-13" version="1">MATAKPILYGSWISSCSHRVRIALNLKGLDYEYKAVNPLTDPDYEKINPIKYIPALVDGDIVVSDSLAIILYLEDKYPQHPLLPKDLKTKALDLQIANIVSSSIQPLQGYGVIGLYDGRLSPDESLQVVQHYINKGFRAIEKLLEGCDSKYSVGDEVHLGDVYLAPQIHAAINRFQIDMTKYPILARFHDAYMEIPAFQAALPQNQPDAPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IUY3</accession>
    <name>I1IUY3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC104584803</name>
        <name evidence="7" type="ORF">BRADI_4g44205v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
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        <property type="gene ID" value="BRADI_4g44205v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_4g44205v3"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL21B"/>
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    </dbReference>
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        <location>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OBAP family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="I1IXV8"/>
    <dbReference type="STRING" id="15368.I1IXV8"/>
    <dbReference type="EnsemblPlants" id="KQJ82685">
        <property type="protein sequence ID" value="KQJ82685"/>
        <property type="gene ID" value="BRADI_5g10450v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835371"/>
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        <property type="gene ID" value="BRADI_5g10450v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835371"/>
    <dbReference type="eggNOG" id="ENOG502QR3B">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="236" mass="26131" checksum="07DFCC588EC8C548" modified="2012-06-13" version="1">MAASCHDVDVPGKPTETGTALLETATGAIQGFAPINQIHQHLCAFHFYGDDMTRQVEAHHFCAHLNEDMRQCLIFDGPDAGARLIGLEYIVSEELFLTLPDEEKPLWHTHEFEVKSGVLFMPGVPGVVERRDLEKVAKTYGKTIHFWQVDRGDALPLGVPQIMMALTRDGQLRQDLADCVEKKYGVSFQKERENRAYMSGPAHGIHPLANAAGKGLKTDIREVDLPTAIAGARIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1J361</accession>
    <name>I1J361_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100831192</name>
        <name evidence="13" type="ORF">BRADI_5g25580v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="I1J361">
        <property type="interactions" value="1483"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J361"/>
    <dbReference type="EnsemblPlants" id="PNT62099">
        <property type="protein sequence ID" value="PNT62099"/>
        <property type="gene ID" value="BRADI_5g25580v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_5g25580v3"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000147">
        <property type="entry name" value="EH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040990">
        <property type="entry name" value="DUF5600"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031692">
        <property type="entry name" value="EHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF145">
        <property type="entry name" value="OS04G0669300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18150">
        <property type="entry name" value="DUF5600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16880">
        <property type="entry name" value="EHD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="10">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="54"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Dynamin-type G" evidence="12">
        <location>
            <begin position="197"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="PNT62099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="548" mass="61676" checksum="FCF92757BA1772EA" modified="2012-06-13" version="1">MASSASASANSSPVRWGLKEQRSVYLRWFYIADDDGDGRVTGKDALKFFAMSNLARDELKQVWAIADSKRQGYLGFAEFMAAMQLVSLAQAGQEISKDTIAHADLDNLQPPTMEGLDKKLKKKSANRSSSDITAYHPTESPLSSSWFNSKSGKKITLKSVTSIIDGLKKSYIEKLRPLEKTYQYNDFVSPLLTSSDFDAKPMIMLLGQYSTGKTTFVKHLLKTSYPGAHIGPEPTTDRFVVITSGPDERCIPGNTIAVQADMPYSGLSAFGTAFLSKFECSQMPHQLLEHITFVDTPGVLSGEKQRTQRSYDFTGVTSWFAAKCDLILLLFDPHKLDISDEFKRVIGSLKGNDDKIRIVLNKADQVDAQQLMRVYGALLWSLGKVLNTPEVMRVYIGSFNDKPIRETAAGPLGMELFEREQEDLMSDLNDIPKKACDRRINEFVKRARSAKVHAYIIGHLKKEMPALMGKGKAQQRLLETLEEQFDKVQKEMRLPAGDFPSVEEYRDTLSACNFDRFERLRPKMLQAVDDMLAYDIPDLLKQFRNPYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EGZ6</accession>
    <name>A0A0Q3EGZ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NPH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100833208</name>
        <name evidence="7" type="ORF">BRADI_4g08917v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237344.1">
        <property type="nucleotide sequence ID" value="XM_010239042.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237345.1">
        <property type="nucleotide sequence ID" value="XM_010239043.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237346.1">
        <property type="nucleotide sequence ID" value="XM_010239044.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237348.1">
        <property type="nucleotide sequence ID" value="XM_010239046.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237349.1">
        <property type="nucleotide sequence ID" value="XM_010239047.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237351.1">
        <property type="nucleotide sequence ID" value="XM_010239049.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758620.1">
        <property type="nucleotide sequence ID" value="XM_014903134.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758621.1">
        <property type="nucleotide sequence ID" value="XM_014903135.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758623.1">
        <property type="nucleotide sequence ID" value="XM_014903137.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758624.1">
        <property type="nucleotide sequence ID" value="XM_014903138.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318673.1">
        <property type="nucleotide sequence ID" value="XM_024462905.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318674.1">
        <property type="nucleotide sequence ID" value="XM_024462906.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318675.1">
        <property type="nucleotide sequence ID" value="XM_024462907.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318676.1">
        <property type="nucleotide sequence ID" value="XM_024462908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EGZ6"/>
    <dbReference type="SMR" id="A0A0Q3EGZ6"/>
    <dbReference type="EnsemblPlants" id="KQJ87004">
        <property type="protein sequence ID" value="KQJ87004"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ87005">
        <property type="protein sequence ID" value="KQJ87005"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ87006">
        <property type="protein sequence ID" value="KQJ87006"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ87007">
        <property type="protein sequence ID" value="KQJ87007"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT62853">
        <property type="protein sequence ID" value="PNT62853"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT62854">
        <property type="protein sequence ID" value="PNT62854"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT62855">
        <property type="protein sequence ID" value="PNT62855"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT62856">
        <property type="protein sequence ID" value="PNT62856"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT62857">
        <property type="protein sequence ID" value="PNT62857"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833208"/>
    <dbReference type="Gramene" id="KQJ87004">
        <property type="protein sequence ID" value="KQJ87004"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87005">
        <property type="protein sequence ID" value="KQJ87005"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87006">
        <property type="protein sequence ID" value="KQJ87006"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87007">
        <property type="protein sequence ID" value="KQJ87007"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62853">
        <property type="protein sequence ID" value="PNT62853"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62854">
        <property type="protein sequence ID" value="PNT62854"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62855">
        <property type="protein sequence ID" value="PNT62855"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62856">
        <property type="protein sequence ID" value="PNT62856"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62857">
        <property type="protein sequence ID" value="PNT62857"/>
        <property type="gene ID" value="BRADI_4g08917v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833208"/>
    <dbReference type="OrthoDB" id="1699162at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EGZ6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="NPH3" evidence="6">
        <location>
            <begin position="203"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="477"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="507"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ87006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="552" mass="60625" checksum="DD1F549B7F33F666" modified="2016-01-20" version="1">MKYMKLGTKPDTFYTEQAVRSVASDIPADLIIHVNNTQYMLHKFPLLLKCGLLQRLCADTDTDTDEPVPVALDDIPGGPDAFELCAKFCYGIAISVSAGNIVEATLAARFLRMTEQVCKGNLASKLETFFESCVLRGWKDSITALRAAWRASGWSESRIVQPCVDSIVEKILLPPSKVSWSYTYTRPGGRHAAKPSSSSVPKDWWTEDVSELDIELFRSVVSTVRAARLLPPPLIGEALHVYACKHLPDPALNLGGVMDGHEELELAKLRRVVETVVTMIPGDAGAVTGRFLLRLLRVANYVGASSSTRAQLVRQAGAQLDEARPADLLIPLPAASGSGSYDVGAAEAVLEQFLAQSQRRRRTSVAMDKVVRVLDEYLQTIALVDAGFPVASFVDLAGCLPAVARNQHDGLYHAIDTYLKEHPELSKADKKRLCRLIDCRKLSPDVRAQAIANDRMPLRTIVQLLFVEQERTVIGTTAGTSSSHAPPPDRASVSRVTAIDDDDDREEEHKSDVHRPRGDRAERLQRAAMTRSLSASTKTEATDHRGSRLRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EIB9</accession>
    <name>A0A0Q3EIB9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3615 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g11478v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ87487">
        <property type="protein sequence ID" value="KQJ87487"/>
        <property type="gene ID" value="BRADI_4g11478v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87487">
        <property type="protein sequence ID" value="KQJ87487"/>
        <property type="gene ID" value="BRADI_4g11478v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EIB9"/>
    <dbReference type="OrthoDB" id="684795at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33326:SF14">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33326">
        <property type="entry name" value="OS05G0543800 PROTEIN"/>
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    </dbReference>
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            <end position="151"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ87487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="168" mass="19723" checksum="1E1468F764A19CBB" modified="2016-01-20" version="1" fragment="single">EELRRSAKEYRAESEESYHDDCRSNDSDDDYEELSDQQIIENGRKWMSEEVMIAFKKYTENCADQTGLDYQFGELLHQCLNVENYYKTFHHFNFTMKMKDPTSSGWRVELYFAEIMQMFGKKHYFCCPLESLEDGHCYGCKNQGVNDLKHPATGGFDQGCPASTFPYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ELN8</accession>
    <name>A0A0Q3ELN8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g10035v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ELN8"/>
    <dbReference type="EnsemblPlants" id="KQJ87260">
        <property type="protein sequence ID" value="KQJ87260"/>
        <property type="gene ID" value="BRADI_4g10035v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87260">
        <property type="protein sequence ID" value="KQJ87260"/>
        <property type="gene ID" value="BRADI_4g10035v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3ELN8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ87260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="75" mass="8336" checksum="2D88EF2A92777D6E" modified="2016-01-20" version="1">MVCSRPILDAPAPAPALPGVFRDAPSRKKGRKETNFCWGSPHAARSISIAWICIWMQRENLLLLEIGVLVIIIHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EMR9</accession>
    <name>A0A0Q3EMR9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g21611v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ88830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EMR9"/>
    <dbReference type="FunCoup" id="A0A0Q3EMR9">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3EMR9"/>
    <dbReference type="EnsemblPlants" id="KQJ88830">
        <property type="protein sequence ID" value="KQJ88830"/>
        <property type="gene ID" value="BRADI_4g21611v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88830">
        <property type="protein sequence ID" value="KQJ88830"/>
        <property type="gene ID" value="BRADI_4g21611v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EMR9"/>
    <dbReference type="OrthoDB" id="167869at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF51">
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    </dbReference>
    <dbReference type="Pfam" id="PF14303">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="188"/>
            <end position="317"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="234"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ88830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="326" mass="38044" checksum="06A4C2E660A1D5A0" modified="2016-01-20" version="1">MDSQSPMGLYTNLLSEGYTQEAWDQNLGSPFREQVMQSQVTPPVKANTKRTRNFTDKEDEVLVAAWLHASMDPIVGSEQKHATYWNRIHEEYELHKPQGSERNVNSISHHWSVVKEQVGRFCGCYEQIMHRHESGKPEQDKLKLIGHLIVIKSRSQAPMQVQVCLLLELMRYTVDAIHIDDFEATSPVKAGHMKRPIGKKAEKERQRRGKNVTSLEDSNVVMALDVLFSKRTKLEEARDMARETSRQAREMARETARQAREDAREASKEKRYVGALAMEQRKFEFEERKMEMDIMNKDLSSLDDDQKEYYKMIRRDIIDRQSKRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ER36</accession>
    <name>A0A0Q3ER36_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g21005v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F635</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BRADI_3g13904v3</name>
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <text evidence="1">Belongs to the ARG7 family.</text>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_3g41415v3"/>
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        <property type="gene ID" value="BRADI_3g41415v3"/>
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        <source>
            <dbReference type="EMBL" id="KQJ99150.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ99150"/>
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    </evidence>
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</entry>
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    <accession>A0A0Q3GM73</accession>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_010238459.3"/>
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    <dbReference type="STRING" id="15368.A0A0Q3GM73"/>
    <dbReference type="EnsemblPlants" id="KQK12224">
        <property type="protein sequence ID" value="KQK12224"/>
        <property type="gene ID" value="BRADI_1g02280v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842077"/>
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        <property type="protein sequence ID" value="KQK12224"/>
        <property type="gene ID" value="BRADI_1g02280v3"/>
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    <dbReference type="KEGG" id="bdi:100842077"/>
    <dbReference type="OrthoDB" id="654677at2759"/>
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        <property type="entry name" value="probable receptor-like serine/threonine-protein kinase At5g57670"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987:SF5">
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    <dbReference type="Pfam" id="PF00069">
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    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="588"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="209"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="316"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="431"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="279"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="385"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="616"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK12224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="927" mass="100054" checksum="3B3D59DF3E2CF368" modified="2016-01-20" version="1">MKVSKKQREQEEEGAMDSAGAGVGKGAEEGVAGGGGGGGTVVVGVRADAESRALLTWTFVNAVAAGDRVVAVHVVLASGAEAAAAVDFDSMLAVYEGFCNLKQINLKVKICKDSSVRKALVREATLFGASKVVLGITKKKRAIASSLSAAKYCAKKLPAKCAILAVNSGKIVYRREANGHSGKVSAEVPGCGEDEMYCVLPFRASEVKESTLPCDEPKDGGGGDEAQHDVGTMGSQSEEIVSEEQRPSIVKPSELLTDQVQSDADSSVKAEEVQNDADPSGKAEESTMDQKDDISDLPGEGASVLYCVLPARDDHSVASSSSRQHNDSIDPPAEVDGELYCILPPRNGHSGRSSNGSKRSTISQKDDNSASLSPGGDGELYCRLSGNRRSGGSSGGSKRSVGVRGMIRAIRRSSSFSSDIQFNLEASADKRDDSVCTSATERTSSTASTELEDLPKDSAHNVETPSSSPMSLRRMIEGRSDRCRLRRRIFNHQRSSSFEWAKVSMVQWAMRLPSRYNSIHPDNKSLKSDASSMLECESTSPVEPESTFSFSLYDVAWPPSELESLKEKYYSVCRLFSYEELKLATSNFSPDMLIGKGGTSHVYKAQLVDGTLYAAKILKPSVDALQEFITEVETVTSLQHENIVSLRGFSFDNYSLVLVYDYMHQGSLDKALHGKCENSLSWEKRNKIAIHIATALEFLHHGGLTLSVIHGDVKSANILLSENFQAQLCDFGLAKHVSASTPHLTCTDITGTFGYLAPEYFSHGKVNEKIDVYAFGVVLLEIISGRRPITTGCAKGQESLVGWARPLLSSGEIKQVVDPVLGNNYDCDEMERMTLAASLCTRMSSYARPETPLVLKLLQGDDETIHWARSQLTAGSDVSDEEAVTPGSNMQSHLNLALLGVDEDDTISRSSTEQTVDTYWSRSSSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GP08</accession>
    <name>A0A0Q3GP08_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS2c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">30S ribosomal protein S2, chloroplastic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_1g05788v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GP08"/>
    <dbReference type="FunCoup" id="A0A0Q3GP08">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GP08"/>
    <dbReference type="EnsemblPlants" id="KQK12760">
        <property type="protein sequence ID" value="KQK12760"/>
        <property type="gene ID" value="BRADI_1g05788v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12760">
        <property type="protein sequence ID" value="KQK12760"/>
        <property type="gene ID" value="BRADI_1g05788v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GP08"/>
    <dbReference type="OrthoDB" id="773813at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.610:FF:000001">
        <property type="entry name" value="30S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.610">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_uS2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK12760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="236" mass="26864" checksum="1297C1FA964707C5" modified="2016-01-20" version="1">MTRRYWNINLKEMIEAGVHFGHGIKKWNPKMAPYISAKRKGTHITNLARTARFLSEACDLVFDAASQGKSFLIVGTKKRAADLVASAAIRARCHYVNKKWFSGMLTNWSITKTRLSQFRDLRAEEKMGKFHHLPKRDVAILKRKLSTLQRYLGGIPYMTRLPDIVIVLDQQKEYIALRECAILGIPTISLVDTNCDPDLANISIPANDDTMTSIRLILNKLVFAICEGRSLYIRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GSD6</accession>
    <name>A0A0Q3GSD6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g09290v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13309.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GSD6"/>
    <dbReference type="STRING" id="15368.A0A0Q3GSD6"/>
    <dbReference type="EnsemblPlants" id="KQK13309">
        <property type="protein sequence ID" value="KQK13309"/>
        <property type="gene ID" value="BRADI_1g09290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13309">
        <property type="protein sequence ID" value="KQK13309"/>
        <property type="gene ID" value="BRADI_1g09290v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GSD6"/>
    <dbReference type="OrthoDB" id="10536535at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GSD6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046533">
        <property type="entry name" value="DUF6598"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065:SF121">
        <property type="entry name" value="DUF6598 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065">
        <property type="entry name" value="OS07G0486400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20241">
        <property type="entry name" value="DUF6598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6598" evidence="1">
        <location>
            <begin position="144"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK13309.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="210" mass="23471" checksum="32AF014E4C1E7272" modified="2018-03-28" version="2">MASTAELLLRASGATLWLPLPSHSAPYPSSCSEDLGDLLDQLEEIELDHHSKPPRMVTGKDYTKSEAVDIEIEYMDRYCERMGVYLAMRDAIIAPEDYDTPFPPFPLKVLPPATSACFVDGCCYHSVYKTHDTSTNHNVCYSSSLCVCQALEPSYPTSVYGIFAVRDDLDKRRNYVFNCPRYAAVGIGKQDSFALPLCSPCRGMYVLDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GXM3</accession>
    <name>A0A0Q3GXM3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst component Exo84 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC104584265</name>
        <name evidence="6" type="ORF">BRADI_1g24297v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EXO84 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="15368.A0A0Q3GXM3"/>
    <dbReference type="EnsemblPlants" id="KQK15672">
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        <property type="gene ID" value="BRADI_1g24297v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584265"/>
    <dbReference type="Gramene" id="KQK15672">
        <property type="protein sequence ID" value="KQK15672"/>
        <property type="gene ID" value="BRADI_1g24297v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584265"/>
    <dbReference type="OrthoDB" id="1710909at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:Golgi to plasma membrane transport"/>
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    </dbReference>
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        <property type="term" value="P:intracellular protein localization"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Exo84p, C-terminal helical domain"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </evidence>
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        <source>
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</entry>
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    <accession>A0A0Q3H687</accession>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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            <dbReference type="EMBL" id="KQK18423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18423"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
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    </dbReference>
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        <location>
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            <end position="56"/>
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        </location>
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            <end position="111"/>
        </location>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
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    <name>A0A0Q3H896_BRADI</name>
    <protein>
        <submittedName>
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        </submittedName>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ84443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3H896"/>
    <dbReference type="EnsemblPlants" id="KQJ84443">
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        <property type="gene ID" value="BRADI_5g20910v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ84443"/>
        <property type="gene ID" value="BRADI_5g20910v3"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33320:SF30">
        <property type="entry name" value="OS04G0606200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ84443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="75" mass="8004" checksum="CD6EB49150DACAB0" modified="2016-01-20" version="1">MPAAAMAVKVICGAEESRVVGTGKAPGACPCCGGPVVATDVESERRILFCLPLCLKNKRKYSCSRCLRRLVTLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HLL6</accession>
    <name>A0A0Q3HLL6_BRADI</name>
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        <recommendedName>
            <fullName evidence="5">Knottin scorpion toxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK23807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A0Q3HLL6">
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    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK23807">
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        <property type="gene ID" value="BRADI_1g76294v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK23807">
        <property type="protein sequence ID" value="KQK23807"/>
        <property type="gene ID" value="BRADI_1g76294v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HLL6"/>
    <dbReference type="OrthoDB" id="612953at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036297538" description="Knottin scorpion toxin-like domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK23807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="7864" checksum="A6646A139E4E6397" modified="2016-01-20" version="1">MCFLVLFVLHGNPATMAEKCGYRLLPLSFCFDAMCKSECWIEGVALGAQVKEYECGGPGPKTYCICLFCQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HWL0</accession>
    <name>A0A0Q3HWL0_BRADI</name>
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        <recommendedName>
            <fullName evidence="7">Cyclin</fullName>
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    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100835427</name>
        <name evidence="4" type="ORF">BRADI_3g33590v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin U/P subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="KQJ97834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HWL0"/>
    <dbReference type="STRING" id="15368.A0A0Q3HWL0"/>
    <dbReference type="EnsemblPlants" id="KQJ97834">
        <property type="protein sequence ID" value="KQJ97834"/>
        <property type="gene ID" value="BRADI_3g33590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835427"/>
    <dbReference type="Gramene" id="KQJ97834">
        <property type="protein sequence ID" value="KQJ97834"/>
        <property type="gene ID" value="BRADI_3g33590v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HWL0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="29743" checksum="E87EC4130C8C7B99" modified="2016-01-20" version="1">MPTKQQISFSFFNLPSSLPLAFSTFPPFITTSTTTPPSRSLSLSVSDRPTTWPATNKMMAAGDQEKQAPVPRVVSILSALLERVAERNDVVAAAPEKEKKEEKAVSAFQGLTKPAISVGVYLERIFRFAGCSPSCYVVAYIYLDRFLRRRPALAVDSFNVHRLLITSVLTAVKFVDDICYNNAYFARVGGISLMEMNYLEVDFLFGVAFDLNVTPATFDSYCSVLQAEMAFLDHPPPSPVDDVSPALLQHQDQVDAGRHRHEQQQLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HZZ7</accession>
    <name>A0A0Q3HZZ7_BRADI</name>
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        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_3g41543v3</name>
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    <organism evidence="8">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="KQJ99170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HZZ7"/>
    <dbReference type="FunCoup" id="A0A0Q3HZZ7">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3HZZ7"/>
    <dbReference type="EnsemblPlants" id="KQJ99170">
        <property type="protein sequence ID" value="KQJ99170"/>
        <property type="gene ID" value="BRADI_3g41543v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99170">
        <property type="protein sequence ID" value="KQJ99170"/>
        <property type="gene ID" value="BRADI_3g41543v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HZZ7"/>
    <dbReference type="OrthoDB" id="786597at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HZZ7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003677">
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ99170.1"/>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ99170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="289" mass="30868" checksum="D9045782612CB317" modified="2016-01-20" version="1">MSPLPLPPPPERRRRGDDGGGEYYYHGEAPEVVGEAAQGGGGRRRSCAEHELDVMVAALTHVISSGREPLAAAAGAVFEGAVSRQQVTTCQQFYSVAAPPLMARPASVAASTASEEQRAEEQHAETPRYRGVRRRPWGKWAAEIRDPVKAARVWLGTFSTAEDAARAYDTAALRFKGPDKAKLNFPLSSQPLAGVSSHVAPPPRRRPAAASIDDVASGPSGQEELSCFFPDIGRYAHILQSGGGDDDVLRDVHQHVIVGAGSGSAPPGHYSEEEEHRPPSTWREKSAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name evidence="2" type="ORF">BRADI_3g15806v3</name>
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
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                <person name="Mockler T.C."/>
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                <consortium name="EnsemblPlants"/>
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            <begin position="34"/>
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    </feature>
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        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ95211.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="1 2" key="1">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
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        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
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    </dbReference>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ82664"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="42733" checksum="0E3B253A35D01BC4" modified="2016-01-20" version="1">MARGRKFQGISFTSILEDIQQEILARLMAKSVFRCRIVWWAWHRLTSGSRFFLEHHLHQPEFPLVATSSNDTDLGISLGVVDFCVSGIHPLATPPIGYNCQCSIEASCNGLVIIGGYIGNPSTRQWAPFAAHVDSIVVLFLAHQLSSEYRVLFWRYKGVSRPPSPIEVHCPIEYFVLTVGCKNPRVVKCLLMPKMPKIEYELIVFIQNRLFLHWRNRAESICYHKILVFDTLAETFRHTKTPGVKTCDQMQLFDMERVLAAYSSTYRMTGMRIFIMRGHYERHNTFWVFHHRIKLLKFDIRRFQEQGDWWAKIVSKDCDLLVSCFGMLLHFDKSGKLAGKFKYDDDVPVILGQKLKYSLIEHRFVLGH</sequence>
</entry>
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    <accession>A0A0Q3IEE9</accession>
    <name>A0A0Q3IEE9_BRADI</name>
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        <recommendedName>
            <fullName evidence="12">Cytochrome P450</fullName>
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    </protein>
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        <name evidence="9" type="ORF">BRADI_5g21430v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the cytochrome P450 family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQJ84528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581650.3">
        <property type="nucleotide sequence ID" value="XM_003581602.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IEE9"/>
    <dbReference type="EnsemblPlants" id="KQJ84528">
        <property type="protein sequence ID" value="KQJ84528"/>
        <property type="gene ID" value="BRADI_5g21430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828667"/>
    <dbReference type="Gramene" id="KQJ84528">
        <property type="protein sequence ID" value="KQJ84528"/>
        <property type="gene ID" value="BRADI_5g21430v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IEE9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000064">
        <property type="entry name" value="Cytochrome P450 monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF21">
        <property type="entry name" value="OS06G0642300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ84528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="55976" checksum="A47FD8F51EE44F67" modified="2016-01-20" version="1">MSSSTTTTTNPYYLIFLAAVSVLLLLLPSLLLAKLRRRPCKPGAMNPPPGPWQLPVIGSLHHLVGALPHRAMRDLALRHGPLMLLRMGELPVVVASSADAAREVMKTHDAAFATRPRTDTIRTITRDGLGIVLAPHGDHWRQVRKLCVTELLSARRVRSFRGSREAEADSLVASVASASEGKAVNVSYLVATYVANAVVRAVVGGRIEDSDAFLASLEEGAKVAAGFSLPDLFPSSRLARALSGTARRVEAVVGELSRLMDAVIEDKRDRRSGAGDEEEDILDVLLRLQHVDDAPLDIGTIRAVIRDLFGAGSETSATTLHWAMSELMRNPAALRRAQAEVRGVLAGLGHSRVREDALPELRYLQLVIKETLRLHAPVPLLLPRECREPCRVMGYDVPQGAMVLVNAWAIGRDAASWGPDVEEFRPERFEDAVPAVDFKGSDFEFVPFGAGRRMCPGITFGVTVMELALASLLFHFDWELPEGASGLDMEEALGITARRKNNLWLRASVHAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IIF7</accession>
    <name>A0A0Q3IIF7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100825454</name>
        <name evidence="7" type="ORF">BRADI_4g02767v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IIF7"/>
    <dbReference type="STRING" id="15368.A0A0Q3IIF7"/>
    <dbReference type="EnsemblPlants" id="KQJ85992">
        <property type="protein sequence ID" value="KQJ85992"/>
        <property type="gene ID" value="BRADI_4g02767v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ85992">
        <property type="protein sequence ID" value="KQJ85992"/>
        <property type="gene ID" value="BRADI_4g02767v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="651300at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IIF7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038920">
        <property type="entry name" value="At3g05675-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058039">
        <property type="entry name" value="At3g05675-like_ankyrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31060:SF29">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31060">
        <property type="entry name" value="OSJNBA0011J08.25 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25553">
        <property type="entry name" value="BTB-POZ_ANK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="130"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="At3g05675-like ankyrin-like" evidence="6">
        <location>
            <begin position="281"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="37"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ85992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="578" mass="64240" checksum="CD99DA4BC2212D76" modified="2016-01-20" version="1">MEESRPRRAPARIRAVPIAVTPEGFWCCPSPAALHKSLKNPHHHHHHHGSNNGGGHGKQPRKIPSAPPSKAPSVQTAPSVADESGRREGAGEQDAPAVEAAAADGREEPAGGGEAQLQQHEICVGFGQPETSDLTVMLYGKEGIAVRMNVHRDVLCGSSAFFAAKLSGGGNGGPPVPSVEIHDCDDAEIYVETVGLMYCDEAKHKLLKQNVSRVLRIMKVAESLGFEACVKSCLDYLEAVPWVGEEEDNVVSSIRHLQSKAYGVSPLLKRVASDSLNSPADTLSHIMEMVLKSNDDRGRREMKALVLNLLKDSNRCTDGTSDICSELFHSSCQGCIDRLKLLFAEASEAGFPSQVTRQITLETDNLLWLVEILVNQRICDDFVVMWASQSELASLHAKLSVASRHAVSCITARLFVGIGRGEMLPSKNTRLLLLQVWLQALIDDYSWLQCSCRSFDRKLVEEGIGQTILTLPLEDQRSILLSWLGRFLKLGDNCPNLQRAFEVWWRRTFVRPYVNQARWWMDELAVRGCWGYGQDKERSYQLPMTMPVYINFSGTQMLSVSWNQWKLRLRDVMESDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IR47</accession>
    <name>A0A0Q3IR47_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g40953v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IR47"/>
    <dbReference type="EnsemblPlants" id="KQK08291">
        <property type="protein sequence ID" value="KQK08291"/>
        <property type="gene ID" value="BRADI_2g40953v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08291">
        <property type="protein sequence ID" value="KQK08291"/>
        <property type="gene ID" value="BRADI_2g40953v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IR47"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK08291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="80" mass="8375" checksum="FBFB5F082FCC66FA" modified="2016-01-20" version="1">MLFVDDLYSCFCRLSNVPCGGGGVCSASAVLRQLLTVCFCCTFGTLVAILLSSLVSSMAILSLIVSERSDVGLPYLAATM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IXC4</accession>
    <name>A0A0Q3IXC4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BRADI_2g18110v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="5"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IXC4"/>
    <dbReference type="FunCoup" id="A0A0Q3IXC4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3IXC4"/>
    <dbReference type="EnsemblPlants" id="KQK05116">
        <property type="protein sequence ID" value="KQK05116"/>
        <property type="gene ID" value="BRADI_2g18110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05116">
        <property type="protein sequence ID" value="KQK05116"/>
        <property type="gene ID" value="BRADI_2g18110v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IXC4"/>
    <dbReference type="OrthoDB" id="9977870at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22582">
        <property type="entry name" value="BRcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000230">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="283"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="287"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK05116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="475" mass="51805" checksum="D85595E5E4BE738F" modified="2016-01-20" version="1">MAGGDELAALHEQAVLASCAAVSASDLDLAFQLQVAEAIQASLRTNGNPSSSNAATAVASSSFQPAPALESSDVAYAMAIQAADACRAHHALAAASVRVAAHDALFARELAAVPEDKWARDGDNIERPLDPARPLFRVFFKGMSSKEMVGPRERDPGVAVLAVAVCGPRGEVVLRIQKPVEASVGGRMTLEVLALNEALHAALGLGIRSIKIVTDYKLLGIWRPAQKKLADMINQVLLVRKNFEKCEILLVERSQLQYGRKLAMDSLGSQIAKAIAVNAGMGMRENCPICLEDTDVSKIHAVEGCSHRFCFSCMKEHVKVKLLHGMLPACPQDGCTKQLTVEGSKVFLSPRLLGIMVQRIREGQIPPTQKVYCPYPKCSALMSLSEVIQPMEASSSKYTAADAATLRKCIKCRGSFCIRCKVPWHDRLTCYQYKIMYPDAQSEDAKLKNLAKHWMWRNCAKCNHMIELAEGCYHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JMZ5</accession>
    <name>A0A0Q3JMZ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_1g09877v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13396.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JMZ5"/>
    <dbReference type="FunCoup" id="A0A0Q3JMZ5">
        <property type="interactions" value="366"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3JMZ5"/>
    <dbReference type="EnsemblPlants" id="KQK13396">
        <property type="protein sequence ID" value="KQK13396"/>
        <property type="gene ID" value="BRADI_1g09877v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13396">
        <property type="protein sequence ID" value="KQK13396"/>
        <property type="gene ID" value="BRADI_1g09877v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JMZ5"/>
    <dbReference type="OrthoDB" id="6119954at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010179">
        <property type="term" value="F:IAA-Ala conjugate hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009850">
        <property type="term" value="P:auxin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08017">
        <property type="entry name" value="M20_IAA_Hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000001">
        <property type="entry name" value="N-acetyldiaminopimelate deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044757">
        <property type="entry name" value="ILR1-like_Hyd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF99">
        <property type="entry name" value="IAA-AMINO ACID HYDROLASE ILR1-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="6">
        <location>
            <begin position="354"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK13396.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="566" mass="60174" checksum="0C5CDB2FDB140F53" modified="2018-03-28" version="2">MASPTSFLALPTLQHGSASATEVDGVEHVGDGGDDGHGHIGDRHGADGFRERVERHGTEGDGGGGGCSRCWIQRHRRRHRRRAGGARRRQGGRGGGGGGEERDSMEVAEVRAAADGEDRPERTEARSGAKGGKAACEREMESEEREAEMEGQWRRKAGLQGMATALGRKDLLDEARAPGFAGWVRGLRRRIHQHPELAFQEHRTSALVRAELDAIGIAYAWPVARTGVVATIAGRGGAGSGPVFALRADMDALPIQEMVEWEFKSQEDGKMHACGHDAHVAMLLGAAKLLQSRKDDLKGTVKLVFQPAEEGHAGGYHVLQEGVLDDVDAIFAVHIDPCLPVGTVGSRPGPFLAGSARFRATIAGKGGHAAVPHAAVDPVVAASSAVLSLQQLVAREIDPLESAVVSVTFIKGGSAFNVIPESVTLGGTCRSMTTQGLSYLMKRIREVIEGQAAVGRCAAAADFMEEELRPYPATVNDEAVYAHAKSVAEGMLGECNFRLCPQVMAAEDFGFYAEKIPAAFFSVGVRGGEDEEISHVHTPHLVIHEDVLTVGAALHAAVAIEFLNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JP69</accession>
    <name>A0A0Q3JP69_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g48965v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JP69"/>
    <dbReference type="EnsemblPlants" id="KQK19547">
        <property type="protein sequence ID" value="KQK19547"/>
        <property type="gene ID" value="BRADI_1g48965v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19547">
        <property type="protein sequence ID" value="KQK19547"/>
        <property type="gene ID" value="BRADI_1g48965v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JP69"/>
    <dbReference type="OrthoDB" id="642536at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF71">
        <property type="entry name" value="F-BOX_KELCH-REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="146"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK19547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="411" mass="45038" checksum="F8FF89B9014EF67B" modified="2016-01-20" version="1">MAPTKPAAKTAVKNSDGDAADIPLHLTEKILCGISPLESARYLTVCKSWASTIFSRLAKPCPHLLALQGLGPQPGEAPRRRGSIFSLPIDGSKELNPVMRAKLPIMKSHAHANHMKLSGALPNGCVSFAFPNADAAQGGRSPARSNRVGLVNPVTGAAQSVEVYRKERLFGFQPKVRAAAGAEAFFVSEVSSDSEKRIYLLWRPEGEQEWPARIRLPMASMRQHQFSHSTDAMDLVAYADGAFYAMEFLGFTFVVDTRAPPPMRMARLNTRSILEQYTAISGTGFLRSCHMLVSEGEVLFVGPVFRLDVEGERWVKVESLASDTALFVSEQSSFSVRASETPGCRSNCIYFLTELGDKYSNGTCNWGVYSMEEEKVLFQSSVGSPGKYIAARWFLPRVGTAPQHVPVQPME</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K0X2</accession>
    <name>A0A0Q3K0X2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_1g74135v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23483.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K0X2"/>
    <dbReference type="FunCoup" id="A0A0Q3K0X2">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3K0X2"/>
    <dbReference type="EnsemblPlants" id="KQK23483">
        <property type="protein sequence ID" value="KQK23483"/>
        <property type="gene ID" value="BRADI_1g74135v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK23483">
        <property type="protein sequence ID" value="KQK23483"/>
        <property type="gene ID" value="BRADI_1g74135v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K0X2"/>
    <dbReference type="OrthoDB" id="1047602at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K0X2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080115">
        <property type="term" value="F:myosin XI tail binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007656">
        <property type="entry name" value="GTD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039306">
        <property type="entry name" value="MYOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31448:SF45">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31448">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04576">
        <property type="entry name" value="Zein-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51775">
        <property type="entry name" value="GTD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="GTD-binding" evidence="8">
        <location>
            <begin position="377"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="519"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="572"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="447"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="607"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="576"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="589"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK23483.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="671" mass="75701" checksum="D29147299AD9E9E3" modified="2018-03-28" version="2">MAVKARARSREFSRQFWSVLRHALSECFLIVMLLVVAVVSYTATKFAHICRIRSPCMLCSRLDQVLHGKAWFSEESICAAHSKLAHSDDLCEKFFLSCSESVRKPGNLKNMSVKEKVNSRLRFRHTELCSCCSKPFKKKRDAHRLSEEVSDMFLDDSMSKVKERSIAMASVGCSSDDDVDQLPHGCYRKLSADHDSESEIRISDDDDGCLAMVYEAKQRTRDKPCADAWLQPMITSTNTLSPSPSESTDQTKFMSITPLVPLDTAEGTTNAAKSSDPAIGDGLEEINWGQPNVSNNNLDVQLNTVPEQDCQELQKEKRSPDEEATKIFATSAYDGTSSTADPQHRKKTFLSQLSSARCFDGPWSEVSASPRISIQIDEYSQSDATGSRQFLDLEPTDAHVTSEDEGEISLELSKKKLSVLYKDEAMAMINRLQQDKAAMHMEAMQYLRMMEEQADHDQEAIEKLNDLLTEREKEILDLEAELDNYQIKYSDESFDVRKFDATDGDAKILESLSRLEETLGMPSTNRFDMGGTNDNLQNGTLRDHPTGDGQYVENSELESQCSQLLGEHLNDEPVTSVPSQRNVENQSSESEKDFDSSRLDDDQISSITSIRQEISLLNSRFKALEADQKFLKQILGSLKCSSDGLQYMQEITSHLRQLRRMMTEQRDRTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3N4F6</accession>
    <name>A0A0Q3N4F6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="13">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100833052</name>
        <name evidence="16" type="ORF">BRADI_2g60755v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="13"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753921.1">
        <property type="nucleotide sequence ID" value="XM_014898435.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3N4F6"/>
    <dbReference type="STRING" id="15368.A0A0Q3N4F6"/>
    <dbReference type="EnsemblPlants" id="KQK11542">
        <property type="protein sequence ID" value="KQK11542"/>
        <property type="gene ID" value="BRADI_2g60755v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833052"/>
    <dbReference type="Gramene" id="KQK11542">
        <property type="protein sequence ID" value="KQK11542"/>
        <property type="gene ID" value="BRADI_2g60755v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3N4F6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007127">
        <property type="term" value="P:meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21036">
        <property type="entry name" value="WH_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000307">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047417">
        <property type="entry name" value="WHD_MUS81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21136">
        <property type="entry name" value="WHD_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 13" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MUS81 winged helix" evidence="15">
        <location>
            <begin position="22"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="117"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK11542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="148" mass="16326" checksum="513E8BED3FF83A03" modified="2016-01-20" version="1">MKDSFPGSIPGKKRRRTKCYVPQKNSASYAILITLYREMTRGKAFMMKQELSDAADASGLTQFAIETNPGCSQNDYYTGWSCMKTLLSNALVVKWSNPAKYRLTEEVEKLLSIVSARSGSAAKEISLSDSDSDSDSDELHEGNKPLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ND00</accession>
    <name>A0A0Q3ND00_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BLE2 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g19460v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14910.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ND00"/>
    <dbReference type="STRING" id="15368.A0A0Q3ND00"/>
    <dbReference type="EnsemblPlants" id="KQK14910">
        <property type="protein sequence ID" value="KQK14910"/>
        <property type="gene ID" value="BRADI_1g19460v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14910">
        <property type="protein sequence ID" value="KQK14910"/>
        <property type="gene ID" value="BRADI_1g19460v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3ND00"/>
    <dbReference type="OrthoDB" id="682451at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33115">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33115:SF73">
        <property type="entry name" value="OS07G0649300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK14910.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="797" mass="89873" checksum="70D9639A1E994DEF" modified="2018-03-28" version="2">MANVAEHRVQMMQPATPTAGKKDAGKPEKRLNRFVRLVVLLERLGNALGTLAFTRATVILLGGYPTVLADKDFWFATALIFMEAASIGNDLKIHKLSFKDSTAKLTLYLVLCVVVLLVIISRMQFPAMMMSIFQGKYNRFAITASIVSVLVIVSFGNLQIPAAVARIVLAMMRLIQQHYYGDQENVDKEYLEDRTHLVPSLSIFYAMVLGQGILYIVPGILEIFSFIPRRSLIRRAGFKGHLGVEYVNLYYKYAFEKFMEGAVSASKKITIVIFAMDSLESDLPKKQLCGLQILYSVLRKDPLKTKAVSELTTSMKTMTCLINMLGWTNEKDEDIRSFAAKVTAELADNLRKIKDPLFYIGSPEAKQHNLIQQVHRDERTAPMLKWLKQMAVYCLIPREEPTKMDEQNICVLRCWKRFTKRWSIPEEEPSTDQHLLPVLGMLILDKLARFDIENCVETCRATGHISKIIDFTSNKTDMTNINETQETLLKGSSLKLLRRLASTEGNFGVILRQKISEHPFLLNNLAEILDDGGSSQELKELTAGLLRNLAMDENMKAEIGHMPLIISRLLHAFLSKGAPSSTDSELLRMIAGKPLAVLAVESANNCLVMLAEPGYVFIKELTIMIHDDKYRYVASSLLQNMCVHARPELGNSDLKEVSYILREVLEGIMDAEGAELEVLVGLSSQICNVIPEDFTRELEHGQIKERFMERLVNALNSNKIPSAHCPGIRRARVEHVIYMMKCDPSYAVCFRKCCMMVQRTHSRAENYRTFSGDAGLMEHSIPLSTLVARAKELMSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NHB5</accession>
    <name>A0A0Q3NHB5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_1g30801v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NHB5"/>
    <dbReference type="EnsemblPlants" id="KQK16814">
        <property type="protein sequence ID" value="KQK16814"/>
        <property type="gene ID" value="BRADI_1g30801v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16814">
        <property type="protein sequence ID" value="KQK16814"/>
        <property type="gene ID" value="BRADI_1g30801v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NHB5"/>
    <dbReference type="OrthoDB" id="600709at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF7">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="12"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="246"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="298"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK16814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="464" mass="51462" checksum="520664EBEFAE16B6" modified="2016-01-20" version="1">MASRGEFWSGRRVLAIDANHDSLKALVSALRHLDYNVTPVMDAKTALQTLWEAKEPFKLAIGEVEMPDTNGVDLLRYIKLQTGIPVIAVTANMDTETVMKGMEHGVCNYLLKPVRVEQLRNICQHALRRNPAAWNDEGDANQRVQSAGIVEGDPGPKSTSMKNSERIIDGDADSDENMGLKHSRRPRVTWTKELHDKFEDAYHQLGKADAVPKKICEMMNVDYISRLTVASHLQKYRAYLRRISDPKARASRRRNSSMSNLESSRHYVENRIPRPSSVVASSSSNNPFTTRPIEPRKNLGKKKAVKHGARSRKSKDHAKALCGFLLNTNQGKACSHLDYPFENMPDEETLQPVNQLPVQPPELVSQPSVMMNAPAFFQKQDAPATNLVGALSDASKYFPNLVGNCNPWQTALPSMFPGPSQVNIPEISQLTSFAGTSSSQMPMVTNEMSNPVAAFISSSTPGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NMS6</accession>
    <name>A0A0Q3NMS6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HhH-GPD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100825895</name>
        <name evidence="3" type="ORF">BRADI_1g44750v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564024.1">
        <property type="nucleotide sequence ID" value="XM_003563976.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NMS6"/>
    <dbReference type="STRING" id="15368.A0A0Q3NMS6"/>
    <dbReference type="EnsemblPlants" id="KQK18796">
        <property type="protein sequence ID" value="KQK18796"/>
        <property type="gene ID" value="BRADI_1g44750v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK18798">
        <property type="protein sequence ID" value="KQK18798"/>
        <property type="gene ID" value="BRADI_1g44750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825895"/>
    <dbReference type="Gramene" id="KQK18796">
        <property type="protein sequence ID" value="KQK18796"/>
        <property type="gene ID" value="BRADI_1g44750v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18798">
        <property type="protein sequence ID" value="KQK18798"/>
        <property type="gene ID" value="BRADI_1g44750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825895"/>
    <dbReference type="OrthoDB" id="5607at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3NMS6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47203">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47203:SF1">
        <property type="entry name" value="HYPOTHETICAL BASE EXCISION DNA REPAIR PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="2">
        <location>
            <begin position="86"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK18798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="277" mass="30730" checksum="3CFA14DB73E5DC57" modified="2016-01-20" version="1">MTRKPKRKLPASPARSDSTSLEPYRGHVSPSPAQCLAVRDALLAFHGFPDEFALFRLLRLGLSPENEADPLTERPTVLDGLVTTLLSQNTTDAISRRAFASLKAAFPSWDQVVDEEGKGLEDAIRCGGLAATKAARIRAMLRDVRERRGAICLEYLRELSVDEVKRELSQFKGIGPKTMACVLMFYLQKDDFPVDTHVLRITKAIGWVPSTATREKAYIHLNKKIPDDLKFDLNCLFVTHGKLCPSCTKKLGAQKLKVANATCPLASYCCIAEKIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NZQ5</accession>
    <name>A0A0Q3NZQ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g03559v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81865.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NZQ5"/>
    <dbReference type="EnsemblPlants" id="KQJ81865">
        <property type="protein sequence ID" value="KQJ81865"/>
        <property type="gene ID" value="BRADI_5g03559v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81865">
        <property type="protein sequence ID" value="KQJ81865"/>
        <property type="gene ID" value="BRADI_5g03559v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NZQ5"/>
    <dbReference type="OrthoDB" id="684810at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ81865.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14193" checksum="A87670BE19A4B28F" modified="2018-03-28" version="2">MAEIKMHGWPHGEFCCLWDQVVETALHLVLECPFSKSIWVSFQRDRPRVAAALQDVYIKGWWAILMKIKNGKRNLDSAVASMVAWHIWQERNKRVFKNCSSMPVGVAAVVRSELALLLEIDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PJR6</accession>
    <name>A0A0Q3PJR6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TNase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100822376</name>
        <name evidence="3" type="ORF">BRADI_4g26860v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577905.1">
        <property type="nucleotide sequence ID" value="XM_003577857.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PJR6"/>
    <dbReference type="EnsemblPlants" id="KQJ89625">
        <property type="protein sequence ID" value="KQJ89625"/>
        <property type="gene ID" value="BRADI_4g26860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822376"/>
    <dbReference type="Gramene" id="KQJ89625">
        <property type="protein sequence ID" value="KQJ89625"/>
        <property type="gene ID" value="BRADI_4g26860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822376"/>
    <dbReference type="OrthoDB" id="430293at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3PJR6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF17">
        <property type="entry name" value="STAPHYLOCOCCAL-LIKE NUCLEASE CAN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="160"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ89625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="348" mass="39710" checksum="347B127C0257F353" modified="2016-01-20" version="1">MGNILKRCFSGGEEEDGQYPYYHPASRPHYQPHSPHEQEEGHGVAALAQDLLNFESTASMVPEGLRQHVTASKKAQVKWYQNMLEAYKNTTPPPRTPAEAAQLVATALDWIQRADLEGILEFYGFSIPSLPAASSTHHPQLLPEGVQFVLNTLPVNNKNIGDGDGFTAYVATTDPRESAIVPLEVHEMVIERNQARNRRDYQSADALQNRLNEAGYKILVCSDEEILARKYRIRMRGIDAPELKMAYGKESRNALVKLIGGKRTIIHVYGLDQFERYVGDIYCDNVFIQEQMLKNGHAWYFKKYDKRGEFAKWEREARAAYRGLFVLENPEKPWDWRKKQRNGGIPVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PLW5</accession>
    <name>A0A0Q3PLW5_BRADI</name>
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        </recommendedName>
    </protein>
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    </gene>
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003578135.4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3PLW5"/>
    <dbReference type="EnsemblPlants" id="KQJ90409">
        <property type="protein sequence ID" value="KQJ90409"/>
        <property type="gene ID" value="BRADI_4g31336v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831896"/>
    <dbReference type="Gramene" id="KQJ90409">
        <property type="protein sequence ID" value="KQJ90409"/>
        <property type="gene ID" value="BRADI_4g31336v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831896"/>
    <dbReference type="OrthoDB" id="525027at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="11"/>
            <end position="48"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
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        </source>
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            <dbReference type="EMBL" id="KQJ90409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="635" mass="71119" checksum="0F3C3ADF905EA4B3" modified="2016-01-20" version="1">MTSVFPGAQLYMGKGAESRRGEGNETNQAEASMASSPQQGQAQGGGGGWTAEQFWSLLDKADRRFARVRDLPLFGRHEPEEYGKAFRIYTQLWRMQQEHRHRLLDAGLRRWQVGEIAARIAHLYYSQYQRTSDTALLSEAFVFYHAVLDRAYFLDDHLGGPTKHLRFLARFLLVALLLARRAHTVPRLATNIRALLDESKKTLQEAEYKEWKHVVQEISRFLRADSSFMNMRPLRYSYAFDHPPDTLPTIPPTVKKRGLLLSDAILCSYYHNEVKYTDLTIDTFRMLQCLEWEPCGSFALDNGYSAHDESGQNHPNLLKDLRDAALPPNPLKTVLYRPSVTHFLTVLATKCEELPSNGMMLIYLSAAGEVGSSGFGPDTSERVVSSFNKFDISNTRPINSKEDNEPCLWLGCRESEGSNCIYPCDLIPFTRRPLFLVIDSSVSSTFKSIHGTEKGEATAMLLSPSSRSSSVGFSGDSTRQSGSQFTMFLTAPLQAFCFVIGNNGLDIDRDFYNKAEELLSLSLNEWAMTLVASTSLLPVWIEVLGDPLLRRLLLRFIFCRATLSLLKASNDKVECLPSCVPPLPESVGEESMLSQCCVMRVASFFGATDQFSFSEVTTWPDIEDATSSGGAGKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PR21</accession>
    <name>A0A0Q3PR21_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g40785v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PR21"/>
    <dbReference type="EnsemblPlants" id="KQJ91953">
        <property type="protein sequence ID" value="KQJ91953"/>
        <property type="gene ID" value="BRADI_4g40785v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ91953">
        <property type="protein sequence ID" value="KQJ91953"/>
        <property type="gene ID" value="BRADI_4g40785v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3PR21"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ91953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="210" mass="22583" checksum="2994195A74A8111D" modified="2016-01-20" version="1">MALRGITMDDAIICASLGHNTIINHLTKDQLCLVDGSGLVHRIDNQVICHHIRRNPLSHHLMEQIACLAYLSFLKEAFDDFVIRHHVGGASQLGHPARQHAQSLICPIRVAKSLLEGAEDDPARPQARPEVTGKEGERQVRAARAAAAVHEDAIGAGCGRRHPWADALGDAGVGRPRAGDGDKKVVQGGARPPEWPAASICRTSGVRPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3Q270</accession>
    <name>A0A0Q3Q270_BRADI</name>
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        <name evidence="3" type="ORF">BRADI_3g18745v3</name>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ95729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3Q270"/>
    <dbReference type="EnsemblPlants" id="KQJ95729">
        <property type="protein sequence ID" value="KQJ95729"/>
        <property type="gene ID" value="BRADI_3g18745v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95729">
        <property type="protein sequence ID" value="KQJ95729"/>
        <property type="gene ID" value="BRADI_3g18745v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3Q270"/>
    <dbReference type="OrthoDB" id="584579at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="F-box_dom-containing"/>
    </dbReference>
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        <source>
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            <dbReference type="Pfam" id="PF24758"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ95729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="412" mass="46331" checksum="E4A47BA281F1266F" modified="2016-01-20" version="1">MGVVTTSQEKKRKLGVAKHQGMSHEDVEGIDFISHLPDDIIGTVISFLPTDDAIRTSTLSLECHILSEHMGPIRRLAISNSNLANFYATFDGWLQSPKLNKLQELEFNFGYGFHGPPLPPTTHRFFSTLRVAIMAGCHIHDDIIHAVTISEETIQSVLAGCPALHNLLLSQIAGCRHIQIISGILKIIGLRVGRVWHRLEEIIIQAPSLERLLYLGNCESLQVSLGIAPKLKVLGVLYKRKPSSFEVGPVNKGCLILAWRAQCLVFALTTRQLSLEKVIPFLRIFPCLEKLYYESTDLWGHKNAWRDTNLDDIGCLDLHLKKIVLTNYVGNVSHVNFAMFFLVNAKVLESMRLELWNPENNCRNWIARQSRRLQHEERASSCARVTFATRKSCSKFATIKGACDLSIADPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QHR5</accession>
    <name>A0A0Q3QHR5_BRADI</name>
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            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BRADI_3g54135v3</name>
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    <organism evidence="2">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK01154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QQZ9</accession>
    <name>A0A0Q3QQZ9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1618 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="primary">LOC100833370</name>
        <name evidence="2" type="ORF">BRADI_2g10465v3</name>
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    <dbReference type="STRING" id="15368.A0A0Q3QQZ9"/>
    <dbReference type="EnsemblPlants" id="KQK03890">
        <property type="protein sequence ID" value="KQK03890"/>
        <property type="gene ID" value="BRADI_2g10465v3"/>
    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="bdi:100833370"/>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK03890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="410" mass="44482" checksum="C8DC021A13851E6E" modified="2018-03-28" version="2">MAPPSSSSSASSWVILGSIPRVSNLPADEGDVSVALAAPPRVSILTVSPRVFPARPTPHYFPFVLAADPSGLLLLQANQGCPATREVVDHPGHQSSCWKFSKPHYFVLDAATSSSAFHLPDPSRTNILHQAMLGLLVSPAGAGHFMVAELRPIIGSDKADLLCFSTEVGEWVEKPVHYPLPPRPLAPIGVVSHHGRLWWVDLSWGVIHCDPFADHPVLGFVPFPPDRVLECREGWGVSDNYRCVGVSGGKLRFVDTFMSRRAGGGAPTVSVWTLADPDSNLWALEHEATFADIWADDSYKATGLSRKIPTLATIHPNNPAVVYFFLEEHLFGVDVRARKIVECEIYGLVAPPSLCIASRFVRPWVLPRALSSAGNWSNGLSPAESANARPYQPSPGDYHLVGESRQTFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R1F2</accession>
    <name>A0A0Q3R1F2_BRADI</name>
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            <fullName evidence="9">RNA polymerase III subunit C4</fullName>
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    </protein>
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        <name evidence="7" type="primary">LOC100846711</name>
        <name evidence="6" type="ORF">BRADI_2g34200v3</name>
    </gene>
    <organism evidence="6">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R1F2"/>
    <dbReference type="FunCoup" id="A0A0Q3R1F2">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3R1F2"/>
    <dbReference type="EnsemblPlants" id="KQK07266">
        <property type="protein sequence ID" value="KQK07266"/>
        <property type="gene ID" value="BRADI_2g34200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07266">
        <property type="protein sequence ID" value="KQK07266"/>
        <property type="gene ID" value="BRADI_2g34200v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="747670at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3R1F2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007811">
        <property type="entry name" value="RPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05132">
        <property type="entry name" value="RNA_pol_Rpc4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK07266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37536" checksum="F3B53E3EC7B03D9A" modified="2016-01-20" version="1">MDNKSKVKKEIDGSVAPAPRKVSVCFVLFFYPETDFVQFDAYNICPSHFKGGLKFAPKKPPKKPAKIVPKTEPVEEIKDEAIDKELLMKLKTSQITDPFGRRLKIEKKEEIHTQVAFGQGNSSYARSFPTRHYSAAPKAPKEYVDPWDYTHSNYPVTLPLRKPYSGDPEILDEEEFGESSASRAQDELTAAEELGLMDRSDTPQLLFFQLPSSLPLPNQAQSVEQPNTGSEENAEPASINSMLTCEKTRPSSLTGSKVKDLPGGFMGKILVYKSGKVKMKIGDALFDVSPGANCKFAQEVTAINTRENQCCALGEISKRAVVTPDVEYLLDSIDKMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RC58</accession>
    <name>A0A0Q3RC58_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100842019</name>
        <name evidence="9" type="ORF">BRADI_2g55690v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754583.1">
        <property type="nucleotide sequence ID" value="XM_014899097.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RC58"/>
    <dbReference type="FunCoup" id="A0A0Q3RC58">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3RC58"/>
    <dbReference type="EnsemblPlants" id="KQK10714">
        <property type="protein sequence ID" value="KQK10714"/>
        <property type="gene ID" value="BRADI_2g55690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842019"/>
    <dbReference type="Gramene" id="KQK10714">
        <property type="protein sequence ID" value="KQK10714"/>
        <property type="gene ID" value="BRADI_2g55690v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77201at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RC58">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129555" description="glucan endo-1,3-beta-D-glucosidase" evidence="8">
        <location>
            <begin position="27"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK10714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="402" mass="42818" checksum="55525BB56BE6ED1C" modified="2016-01-20" version="1">MEPRARASSLLLCLMLSLFSVDIVSAQQRFGINYGQIADNLPDPTQVVRLLRSMNVNKVKLYDADSKVLTSFANTGVEFIISVGNENLQTMATTPGAARQWVSQHVQPFIPATRISCVIVGNEVLGNNDNGMAASLVPAMQAIYDALVDLGLSRQVTVSSAHSVNVLASSFPPSSGVFQEGLAQYIKPLLEFHSKTGSPFLINAYPFFAYKGSPGSVSLPYVLFEPNAGVVDPKTNLSYDNMLYAQIDAVYAAMKAMGHTDIGVRVSETGWPSKGDEDEVGATVQNAAAYNGNLMQRIAMNQGTPLKPEVPIDVFVFALFNENMKPGPASERNYGLFYPNGSPVYAINAGTGAGSGPGAGSGSVGRFDPYSSQMFSAASKLAVRTTSLTLLLVLPILSAFSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3REM4</accession>
    <name>A0A0Q3REM4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g61468v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3REM4"/>
    <dbReference type="EnsemblPlants" id="KQK11658">
        <property type="protein sequence ID" value="KQK11658"/>
        <property type="gene ID" value="BRADI_2g61468v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11658">
        <property type="protein sequence ID" value="KQK11658"/>
        <property type="gene ID" value="BRADI_2g61468v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3REM4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK11658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="88" mass="10315" checksum="7418EB279A03BAF7" modified="2016-01-20" version="1">MAFTEAVDLAARFLNIYVHSVLSSDSKLLMQMGGCSFLLKFQLCFVRPINFYRRHKLLYPNGRLMVDVAISFCRCLDQNLWLVCRRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGT5</accession>
    <name>A0A2K2CGT5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100823734</name>
        <name evidence="6" type="ORF">BRADI_5g12557v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239993.2">
        <property type="nucleotide sequence ID" value="XM_010241691.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CGT5"/>
    <dbReference type="STRING" id="15368.A0A2K2CGT5"/>
    <dbReference type="EnsemblPlants" id="PNT61238">
        <property type="protein sequence ID" value="PNT61238"/>
        <property type="gene ID" value="BRADI_5g12557v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823734"/>
    <dbReference type="Gramene" id="PNT61238">
        <property type="protein sequence ID" value="PNT61238"/>
        <property type="gene ID" value="BRADI_5g12557v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823734"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CGT5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000558">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g39710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF13">
        <property type="entry name" value="OS03G0201400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="232"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="266"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="371"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="441"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="476"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="511"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT61238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="601" mass="65770" checksum="C4D64ADB7269464D" modified="2018-03-28" version="1">MRRALRPRQHPPPPSSLPWYAPPPQPPPSHSPSTTEADPLIVAASEVALALPVHPAPLPATAPPPLLRLLPAFTSGHFLSLLRRNPLSLPPLPILSLFRLLLLASPPGLFRHTPSSFLSMSHHLLVHRLPHLARPLLRLLASRLGRSSAPRLLPDLLPAASPGDPAPLVSELAAAYADEGLLPDACSLVLLALRRGISLPAPVCSGLMSRLPSTPEAYTFYLQLLDAGMAPETRLFNVLMRDFVRLGELVSARKVFDEMRRSVQPTVVTFNTLISGMCRARDLDAVDGLYKEMSDVGVKPDVYTYGALIKGFCRTGRMENAVKMFNEMRDTGVNPNAVVFTTLIDAHCKEGNVNAGMDLYQDMRVRGVMPDLVAYNALVNGLCRARNLKAAESIVEEMKNAGLKPDKVTYTTLIDGCCKDGKLDMAMDIKQKMAEKEVSLDEVTYTALISGLSKAGRPVDAERVLREMMEAALEPDNTTYTMVIDAFCRKGDVKTGFKLLKEMQNKGKKPGVVTYNVIMNGLCKLGQMKNADMLLHAMLNIGVSPDDITYNILLDGQCKHGKVANSEELESSKGMVPDFAVYTSLISELAKKKPAKNYHDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CIG0</accession>
    <name>A0A2K2CIG0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g21381v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CIG0"/>
    <dbReference type="EnsemblPlants" id="PNT61824">
        <property type="protein sequence ID" value="PNT61824"/>
        <property type="gene ID" value="BRADI_5g21381v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61824">
        <property type="protein sequence ID" value="PNT61824"/>
        <property type="gene ID" value="BRADI_5g21381v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CIG0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT61824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="60" mass="6664" checksum="0751E4A89CD12873" modified="2018-03-28" version="1">MRSRRAIRASASGVVEKLKNVPMIQGMRGHILRNRGGKKYGNSSTNHIYVLPFPCPCLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CK70</accession>
    <name>A0A2K2CK70_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g03041v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CK70"/>
    <dbReference type="EnsemblPlants" id="PNT62422">
        <property type="protein sequence ID" value="PNT62422"/>
        <property type="gene ID" value="BRADI_4g03041v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62422">
        <property type="protein sequence ID" value="PNT62422"/>
        <property type="gene ID" value="BRADI_4g03041v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CK70"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT62422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="106" mass="12431" checksum="7BB6CDCFD9560C6B" modified="2018-03-28" version="1">MHIVVTPRFNHILSWRIHQSPYPAARTELLSWSNIDPLPSYLSSCRYGRPHRLPSMQIRRHRAPPWSHIPVASLPVVKTSLLFVLNLATFFINRKERARSFVHLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CKC5</accession>
    <name>A0A2K2CKC5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g03987v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CKC5"/>
    <dbReference type="EnsemblPlants" id="PNT62475">
        <property type="protein sequence ID" value="PNT62475"/>
        <property type="gene ID" value="BRADI_4g03987v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62475">
        <property type="protein sequence ID" value="PNT62475"/>
        <property type="gene ID" value="BRADI_4g03987v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CKC5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033311481" evidence="2">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT62475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="100" mass="9917" checksum="55DAE50BBA01A9B9" modified="2018-03-28" version="1">MYISMQGNRRPAGMLLAALLLVAVADAAARGAAHTGGGHATAGMTMTMTMAASLLVSAAGVADSVFNPNKAVCVRLCAAKGPGDSYTGARGCNAYNQCPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CKL5</accession>
    <name>A0A2K2CKL5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g05372v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CKL5"/>
    <dbReference type="EnsemblPlants" id="PNT62574">
        <property type="protein sequence ID" value="PNT62574"/>
        <property type="gene ID" value="BRADI_4g05372v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62574">
        <property type="protein sequence ID" value="PNT62574"/>
        <property type="gene ID" value="BRADI_4g05372v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CKL5"/>
    <dbReference type="OrthoDB" id="685916at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116:SF86">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33116">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="169"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT62574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="282" mass="32330" checksum="98EEB5B566E3FC93" modified="2018-03-28" version="1">MCVPKFKGGMGFRDLFCFNFALLAKQCWRLITNDESLCTRVLRAKYFPDGNLLDVPLKKRASYSWQSIWAGIQTFKRGCIWRVGNGEKINIWTDPWIPSSPTRRIITPRGQTLITSVSDLIDPTTGTWDNALIHDIFWPIDAQRILKVPIARTFVEDFVAWHFNKSGLFSVRSAYHMEWDHQHGEKLRRTNGAGAVQTHPIWRKLRRLKVPLKVKFFGWKGLYNTIPCLATLANRHVPVSAQCPVCPNEVEDLKHVLFTCSRAQEVWHFLGISELIGGTVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CLX1</accession>
    <name>A0A2K2CLX1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g10500v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CLX1"/>
    <dbReference type="STRING" id="15368.A0A2K2CLX1"/>
    <dbReference type="EnsemblPlants" id="PNT63028">
        <property type="protein sequence ID" value="PNT63028"/>
        <property type="gene ID" value="BRADI_4g10500v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63028">
        <property type="protein sequence ID" value="PNT63028"/>
        <property type="gene ID" value="BRADI_4g10500v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CLX1"/>
    <dbReference type="OrthoDB" id="630678at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CLX1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21037">
        <property type="entry name" value="MLKL_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045766">
        <property type="entry name" value="MCAfunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059179">
        <property type="entry name" value="MLKL-like_MCAfunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46146:SF9">
        <property type="entry name" value="OS06G0151700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46146">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE-LIKE PROTEIN CCR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19584">
        <property type="entry name" value="MCAfunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="184"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="545" mass="60812" checksum="AA3AFA9173960788" modified="2018-03-28" version="1">MALWTGLGQAATVAQLVGADIGGLISMIMQAALTARQNRRECEQLARRVFMIAELLPHLQLQDPEAVRPLAGLGDTLRDAHELVLSYQGRSVAYQFVMAGKQADRFREVQSRIDSYLILFPVISYIGITRRLDRIYNILVPDSATSSHSTQLQESAARVAEEVPRHVGAEEFTLAELVEATNNFACDAKLGSGGSAMRGRLHDRREVAVKRYYNHWRNHTELAILSSLRHKHIIRLLGWCTVDKEYKRLLSFRRKEQEKEQLLILEYMENGTLSDHLHRHPSSPVIVSWKMRINVLLGVVRAMEHLHCHAVPPVIHRDIKPSNILFDLSWVPRLSDFGVSVTSDEEPSSIAGTCGYLAPEYPYTGLATPAVDVYSLGVVILEVLTGKAAIFQGERDSQMDLVCFALPVIEDGNYGELLDKRPVPEPTLEQLNVLEHVAQTAACCVQMQWQDRPAISDIVASLEIALQHITLPSTSLAGLCAEVQVVPKEISPGQSRGCMWPQALTLSQAFLEHLVEEAILQTNIVRFAWNSRFQIHASSSGETKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CUB7</accession>
    <name>A0A2K2CUB7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g45170v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CUB7"/>
    <dbReference type="STRING" id="15368.A0A2K2CUB7"/>
    <dbReference type="EnsemblPlants" id="PNT65619">
        <property type="protein sequence ID" value="PNT65619"/>
        <property type="gene ID" value="BRADI_4g45170v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65619">
        <property type="protein sequence ID" value="PNT65619"/>
        <property type="gene ID" value="BRADI_4g45170v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CUB7"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.210:FF:000002">
        <property type="entry name" value="Ribonuclease P protein subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="18"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="33"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT65619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="305" mass="34502" checksum="97278FF7E1F97019" modified="2018-03-28" version="1">MDSSILEIKIRCRAAQPRRSVARLPPRASAATPSRCRAPQPRSSSPSPSHCRAPPLRRSSPSSRHQLKQLPVINKNLLLLQVKKPILYTLNFHLLFMDLDSTDLVQSALYDIIQKGGAGKITKGAKKLKLEKGILLDNYVQRGPRLVDAQSRSLLIHSKRSKRHMSLKQHKKCGSFHLHDTFHRFDLYKPMHEMWKEYMRELTKSTPKKQLAESLLSSDLHGALLIVAQCKAALYEGVSGIMICDTAETFGIISEDNHFRVVPKAGSVFVLQADCWKVTLMGDKLSPKETLKENQRQQRAQSLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CVQ1</accession>
    <name>A0A2K2CVQ1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100826868</name>
        <name evidence="15" type="ORF">BRADI_3g07280v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571078.1">
        <property type="nucleotide sequence ID" value="XM_003571030.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CVQ1"/>
    <dbReference type="FunCoup" id="A0A2K2CVQ1">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66106">
        <property type="protein sequence ID" value="PNT66106"/>
        <property type="gene ID" value="BRADI_3g07280v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826868"/>
    <dbReference type="Gramene" id="PNT66106">
        <property type="protein sequence ID" value="PNT66106"/>
        <property type="gene ID" value="BRADI_3g07280v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826868"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CVQ1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000001">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043158591" description="Subtilisin-like protease" evidence="10">
        <location>
            <begin position="36"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="13">
        <location>
            <begin position="42"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="11">
        <location>
            <begin position="163"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="12">
        <location>
            <begin position="425"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="14">
        <location>
            <begin position="699"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="PNT66106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="800" mass="84335" checksum="E850619B38953189" modified="2018-03-28" version="1">MAGARSSGVRSMAANSLPLAALLICTLLFLDPAAAAGEARSSYVVYLGDHAHGSRLGGLDAADLAALEEKAAGSHHDLLATILGDKDKAREAIFYSYTKHINGFAANLNAAEAAQLARLPEVVSVFPNRAQQQLHTTRSWQFLGLSGPDGVSRGASWRKAKFGEGIIIGNIDTGVWPESESFRDHGLGSVPKNWKGTCEKGQDDKFHCNGKLIGARFFNKGYASGVGAPSDDPTFNSPRDNGGHGTHTLSTAAGAPSPGASVFGLGNGTATGGSPRARVAGYRVCFKPVNGSSCFEADILAAFDAAIHDGVHVLSVSLGGVGDRYDYFEDSIAIGSFHAVRHGITVVCSAGNSGPKPSKISNVAPWMFTVGASTMDRKFSSDVVFNGTKIKGESLSSNTLNQKTPYPMIDSTQAAAPGRSEDEAQLCLKGSLDPKKVHGKIVVCLRGDNARVAKGEVVHEAGGAGMVLANDASSGNEIISDPHVLPATHVGFHDGLLLFSYLKIDKAPVGMIEKPTTSVYTKPAPYMAAFSSQGPSPVNPEILKPDITAPGVGVIAAWTRATSPTELDNDKRRVAYNAISGTSMSCPHVAGIAGLIKALHPDWSPAAVRSALMTTAIEVDNKGQQILNSSFAAAGPFERGAGHVWPSRSFNPALVYDLSPDHYLEFLCALKYNASSMALFSGGGKAAYKCPESPPKLQDLNYPSITVLNLTSSGTTVKRTVKNVGWPGKFKAAVRDPPGVRVSVRPDVLLFAKKGEEKTFEVKFEVKNAKLAKDYSFGQLVWSNGKQFVKSPIVVQTKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CXZ3</accession>
    <name>A0A2K2CXZ3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g17971v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CXZ3"/>
    <dbReference type="EnsemblPlants" id="PNT66880">
        <property type="protein sequence ID" value="PNT66880"/>
        <property type="gene ID" value="BRADI_3g17971v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66880">
        <property type="protein sequence ID" value="PNT66880"/>
        <property type="gene ID" value="BRADI_3g17971v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CXZ3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT66880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="82" mass="9577" checksum="2B0729EB7A2D063F" modified="2018-03-28" version="1">MLYFAPAVRKVPYHAKTIFIQAQSHTNSLRKHFFCVYESVFLSPCEQERPTVRCPSNSKNKNHKPLLPARMKNCNFCGFTDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CY63</accession>
    <name>A0A2K2CY63_BRADI</name>
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        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g19040v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CY63"/>
    <dbReference type="STRING" id="15368.A0A2K2CY63"/>
    <dbReference type="EnsemblPlants" id="PNT66974">
        <property type="protein sequence ID" value="PNT66974"/>
        <property type="gene ID" value="BRADI_3g19040v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66974">
        <property type="protein sequence ID" value="PNT66974"/>
        <property type="gene ID" value="BRADI_3g19040v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CY63"/>
    <dbReference type="OrthoDB" id="431169at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CY63">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Cell wall integrity protein scw1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000037">
        <property type="entry name" value="RNA-binding protein with multiple splicing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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    <dbReference type="SMART" id="SM00360">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="47"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="218"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT66974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="303" mass="32602" checksum="96D0F187FC1761D3" modified="2018-03-28" version="1">MAGAGIHPFHQQWPPAAAASVPPAVPPPPPVPGVPDAAARPGSDEVRTIFITGLPVDVKERELHNLLRWLPGFEASQINFKGDQPMGFALFSYAHHANAAKAALQDLVFDAETKSALHIEMAKKNLFIKRGADANAMDQSKRLRTGGDYTHSPYAPPFHPPPPAVAVWGNAGYLAAPPPYNPYAAYPVPPVPMASPSPLPGPTAYAPVQNTKDNPPCNTLFIGNLGETVVEEELRGLFSVQPGFKQMKVLRQDRNTVCFIEFDDVSAASAVHHTLQGAVIPSSGRGGMRIQYPLLLSFFCFMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CY71</accession>
    <name>A0A2K2CY71_BRADI</name>
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        <recommendedName>
            <fullName evidence="12">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100826244</name>
        <name evidence="9" type="ORF">BRADI_3g19077v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpA/ClpB family.</text>
    </comment>
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        <property type="protein sequence ID" value="PNT66979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234520.2">
        <property type="nucleotide sequence ID" value="XM_010236218.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CY71"/>
    <dbReference type="STRING" id="15368.A0A2K2CY71"/>
    <dbReference type="EnsemblPlants" id="PNT66979">
        <property type="protein sequence ID" value="PNT66979"/>
        <property type="gene ID" value="BRADI_3g19077v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826244"/>
    <dbReference type="Gramene" id="PNT66979">
        <property type="protein sequence ID" value="PNT66979"/>
        <property type="gene ID" value="BRADI_3g19077v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1929681at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CY71">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_SMAX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058680">
        <property type="entry name" value="NBD_SMAX1-like"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051650">
        <property type="entry name" value="SL_signaling_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43572">
        <property type="entry name" value="CHAPERONE PROTEIN CLPD, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43572:SF13">
        <property type="entry name" value="PROTEIN SUPPRESSOR OF MAX2 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26587">
        <property type="entry name" value="AAA_lid_SMAX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23569">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SMAX1-like nucleotide binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase AAA-type core" evidence="6">
        <location>
            <begin position="435"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="SMAX1-like AAA+ ATPase lid" evidence="8">
        <location>
            <begin position="676"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="322"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT66979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="782" mass="84959" checksum="1F05693726B6F59D" modified="2018-03-28" version="1">MLKPTRRNPVLVGDAGPDAVLKEAIRRIPTASSPALAAAKVLQLEAELAQLAGDMAAIAERIGELGAVIERLVGEHGGVVLDLGDLKWLVEGPAAASSEGGKAAVAEMGRLLRRFGRGVVWAVATAACATYLRCKIYHPGMEDEWDLQAVPIARGTPLAGAALRPGGSGILSNSVGMLSPMLRPTPVTPTSLRWPPGGDHTQMSKPVMCMLCKGGYERELAKLVADRVDKPASRPETANPSLPHWLQRSTDQQSKEQELKWKRSTDDLEKKWRETCVRIHSTRAGAPALSMPLASFGPRPPVEPKLAIARSAAIPTLKMNTNREKPTATPTLELHKSPPRSPVKTDLMLGRLDPGTNPSLEKEQKESYEGLTAMQRAKIAGISDIESFKRLLKGLTEKVSWQSDAASAIAAVVIQCRSGSEKRRNLGTRGDMWLLFVGPDQAGKRKMVNALSELMVNTRPVVVNFGGDSQLGRVRNDGLNMGFWGKTALDRVTEAVRQNPFSVIVLEGIDHLDIVVRGKIKRAMETGRLPDSRGREVSLGNVIFVLTTNWIPDELKGPNVETLLQAEGRMLEITSSGWQLELSVVDKQVKHRADWLCDDVRPAKVAKELSSGHGLSLDLNLAVGLVDDTEGSHNSSDVSVEQDQDKGQLAVMCSAPVPDRDLLELVDDAIVFRPVDFCPFRKTITDCLSAKFESVIGSSNAFRIDEDAIDRMAGSIWLNDEKLEDWAGKVLMPSIERLWHNVKRDNARAVVRLASVADKASPRWGGGREGLPASVTIAIDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D912</accession>
    <name>A0A2K2D912_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_2g17140v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D912"/>
    <dbReference type="FunCoup" id="A0A2K2D912">
        <property type="interactions" value="688"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2D912"/>
    <dbReference type="EnsemblPlants" id="PNT70747">
        <property type="protein sequence ID" value="PNT70747"/>
        <property type="gene ID" value="BRADI_2g17140v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70747">
        <property type="protein sequence ID" value="PNT70747"/>
        <property type="gene ID" value="BRADI_2g17140v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D912"/>
    <dbReference type="OrthoDB" id="696656at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF155">
        <property type="entry name" value="OS04G0316900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="106"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="26"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT70747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="661" mass="76506" checksum="2CD92A28C3BA3400" modified="2018-03-28" version="1">MNKYYRINNNMKADFENRPLKKAKCSESSVLDDQLPSPSVSASSLGDDNQTVSADHDKPPDESEHINDGAYDYLPQDYALTELDVCAHVVIEDSSEKEILVKIDQVYVKQCELVCLLDSAKWLNDDQVHVQNKSDTKVYFENTFLAGLLKRDGKYGIHGATFMTEIVGNYLNHDMIHLPVNIEHSHWYLACINVEKSEIQVLDSLCWEHNRVDLTNTLQGLQYHLDILKTQENLSKHNWKDFDVTKWTIREQLHNPIPKDSSSCGLFMLKFMEYWTGHALTHSITQENIIDFRYKLAAVLLCWNTNTAQGDPNDIMMFESLDEENQPNPLNSLSIEKRYQSPISVVSSMSVHELEGGLCNYIKSINSAETLEKVWVQSSDPYTISLTLKRLQGMLNEKLPMERDCFNLVVRKIMFDDIQTLKKRNRLISKHYLDMRFWMITDFGRHPNYRKKLDVEQLACSVCSWPGIKYNVSSCKTVRPLSYTCQHRDFLKSIHLISILFQIHIPIQSNNGFILFVLAKDTRTVYILDPTPIDPIYQRNPYAKYVPRLQWIAEHLPKAMSKTCPGSTWNENIFLWHHHIINNIPIHNRESSGYLITLFMSAWEDEKLNLPFSKDGYELSKQILGKLLTFKKNECEVNMPAGVLDIINSIRNIQTNMNVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D9E7</accession>
    <name>A0A2K2D9E7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100825807</name>
        <name evidence="2" type="ORF">BRADI_2g19460v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566027.2">
        <property type="nucleotide sequence ID" value="XM_003565979.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D9E7"/>
    <dbReference type="STRING" id="15368.A0A2K2D9E7"/>
    <dbReference type="EnsemblPlants" id="PNT70895">
        <property type="protein sequence ID" value="PNT70895"/>
        <property type="gene ID" value="BRADI_2g19460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825807"/>
    <dbReference type="Gramene" id="PNT70895">
        <property type="protein sequence ID" value="PNT70895"/>
        <property type="gene ID" value="BRADI_2g19460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825807"/>
    <dbReference type="OrthoDB" id="675864at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D9E7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33265">
        <property type="entry name" value="AVR9/CF-9 RAPIDLY ELICITED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33265:SF9">
        <property type="entry name" value="OS05G0520600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT70895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="276" mass="30639" checksum="05740D2BF6E74D0F" modified="2018-03-28" version="1">MNRGFEMGSDQEWAAMEEQHDGDGDIDVGRREDGPAAPAALLSGKKLWRMARAVHLVLARGLGKHQPKLAALGVHLHHLLSPMSSSMRHCSFAAPALSCRSVDAVHVHPYPRSGGGRITGGAASSLSCRSMDPGAAVYSKYEYRPREVEFSCSSTPLHSRRRRRRRRSRRRLLQQDEDPEHGSAAAVTRLYDLMDGDEEEEGDVVEISGAGAPRQVRITDSPFPAAREEEDDEAGLVDRRADEFIVWFHGQLRMQQQQQQPQRPAAARELARHVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D9T6</accession>
    <name>A0A2K2D9T6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g22227v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D9T6"/>
    <dbReference type="FunCoup" id="A0A2K2D9T6">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT71052">
        <property type="protein sequence ID" value="PNT71052"/>
        <property type="gene ID" value="BRADI_2g22227v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71052">
        <property type="protein sequence ID" value="PNT71052"/>
        <property type="gene ID" value="BRADI_2g22227v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="682414at2759"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47482:SF5">
        <property type="entry name" value="FAR1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47482">
        <property type="entry name" value="OS11G0632001 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_SWIM"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="561"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT71052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="703" mass="80989" checksum="196D99474B7AE73B" modified="2018-03-28" version="1" fragment="single">MEVGIGSSSACSFASCDNLDGVQRNCRSDVPQCADEASVEEWSEVGAASVEASGLSASSRSPLENALRGFAHRTTENILLPEIGLCFDSLPEAYEYFNLYSWEVRFGIRYGKNKSYKCKSGPKKDGIPKKKNTSSVRCGCKCFIRLLRSEDHGWYIKECRLVHSHKLSRNSGEKMCWPSHKHIDRYTRELVRNLRENNVPLTKVYNVLGTYFGSTEDIPFNKRSLENLCKQISREEANDDVRKTMELFTKMRDEDPSFVYTADLDSDGRIRTLIWVNGKSKLDYIYFGDAITFDTTYKTNLYGMTFGLFVGVDNHFQSIIFSGVLMRQETIESFEWIFREFTSLMGGKAPITILTDQCRAMEVAIERVFTETTHRWCKWHVLKMAKERLGSVYTKYSTFKAEFHKLINYSITVPEFEAAWEELLQKYDLKDHHFLTPIYESRHRWAKPYFTAVFCAKMTSTQRSESANHMLKGYVPPGAPMHLFVKQYNKLLADRISKEDSENQRTRMHAATIYTSVMLEMFSEHIFDSAGYNVIEVVPNKKYLTVHFDASRREKWSKVHYEVTISDDGGQYTCECGLAEHMGMLCCHSIRVMLQLGVDKVPQAHILKRWTKDASDVLPDHLAHLQKDRGSARSQSYRHTSLHVAALELANIGDKNIDCYHDVLKYITDGTKKFGKCTSKQMDEVFKSLLPLQMYIRNQWIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DAT3</accession>
    <name>A0A2K2DAT3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Photosystem II reaction center protein M</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100839994</name>
        <name evidence="9" type="ORF">BRADI_2g26754v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DAT3"/>
    <dbReference type="STRING" id="15368.A0A2K2DAT3"/>
    <dbReference type="EnsemblPlants" id="PNT71378">
        <property type="protein sequence ID" value="PNT71378"/>
        <property type="gene ID" value="BRADI_2g26754v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71378">
        <property type="protein sequence ID" value="PNT71378"/>
        <property type="gene ID" value="BRADI_2g26754v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="564131at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019684">
        <property type="term" value="P:photosynthesis, light reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00438">
        <property type="entry name" value="PSII_PsbM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007826">
        <property type="entry name" value="PSII_PsbM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037269">
        <property type="entry name" value="PSII_PsbM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03038">
        <property type="entry name" value="PS_II_psbM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35774">
        <property type="entry name" value="PHOTOSYSTEM II REACTION CENTER PROTEIN M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35774:SF1">
        <property type="entry name" value="PHOTOSYSTEM II REACTION CENTER PROTEIN M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05151">
        <property type="entry name" value="PsbM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161033">
        <property type="entry name" value="Photosystem II reaction center protein M, PsbM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="7" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="2" id="KW-0674">Reaction center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT71378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="32" mass="3563" checksum="A9E634AB42221197" modified="2018-03-28" version="1">MEVNILAFIATALFILVPTAFLLIIYVKTVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DLM9</accession>
    <name>A0A2K2DLM9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g28484v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DLM9"/>
    <dbReference type="EnsemblPlants" id="PNT75185">
        <property type="protein sequence ID" value="PNT75185"/>
        <property type="gene ID" value="BRADI_1g28484v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75185">
        <property type="protein sequence ID" value="PNT75185"/>
        <property type="gene ID" value="BRADI_1g28484v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DLM9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="283" mass="28935" checksum="74AF84A12BE8855C" modified="2018-03-28" version="1">MSYRAAHLEIHARAKPAAAACVRQIRSSRAGTQTSASEEGGAATAAEQRAAAGAGGTRCGERPEQGAHDAESRGARRRGPSGALRPEQGARDAESRDAAAGAGGPDAEGRGAAAGAGGPDAEGRDAAAGAGGPDEEGRDAAAAEQGGRMRSAGTPRRRSSAMLPEQGARRRSTSTGAESRDAAARCVARLPDRDETAVHSGRSRGAAATPQRRSRGGAREGSANRRKAMEVGDGVQDLPLKEINTTTSSALHRWRPLKWMRSPLFLLSPAAGFFFLASHGGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DM45</accession>
    <name>A0A2K2DM45_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g30651v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DM45"/>
    <dbReference type="EnsemblPlants" id="PNT75346">
        <property type="protein sequence ID" value="PNT75346"/>
        <property type="gene ID" value="BRADI_1g30651v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75346">
        <property type="protein sequence ID" value="PNT75346"/>
        <property type="gene ID" value="BRADI_1g30651v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DM45"/>
    <dbReference type="OrthoDB" id="597900at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46506">
        <property type="entry name" value="OS05G0143600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="79"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT75346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="147" mass="15585" checksum="E6F4E0046FAC3664" modified="2018-03-28" version="1" fragment="single">GEVIDSIAYSYIDQAGKKQTAGPWGGNRGLTFTIVLAPSETIKTISGTTVDFMGNTVVTSLTFVTNVTTYGPFGKANVGEEAHGSADSGARRGPRGLAVGPRGCGGVRGLGGGRIDGERPEEFGTVAEQEQRVRERKTWGRRRAREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQ91</accession>
    <name>A0A2K2DQ91_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chalcone/stilbene synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g48182v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQ91"/>
    <dbReference type="STRING" id="15368.A0A2K2DQ91"/>
    <dbReference type="EnsemblPlants" id="PNT76438">
        <property type="protein sequence ID" value="PNT76438"/>
        <property type="gene ID" value="BRADI_1g48182v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76438">
        <property type="protein sequence ID" value="PNT76438"/>
        <property type="gene ID" value="BRADI_1g48182v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DQ91"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF24">
        <property type="entry name" value="OS07G0526400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="121" mass="13312" checksum="29C23DB82AB63E4A" modified="2018-03-28" version="1">MCEKSGVEKRFFHHTEELLAAHPHFLDRRKPSLDDRLSIVAAAAPALAASAATKAIVNFTWRRITSNNVCRTHSIRCLELPVLNGMTSSGQCTLAVPVPSWTTSTLPSGWIRGSWRRAGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLM6</accession>
    <name>I1GLM6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100840771</name>
        <name evidence="8" type="ORF">BRADI_1g03980v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559246.1">
        <property type="nucleotide sequence ID" value="XM_003559198.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLM6"/>
    <dbReference type="EnsemblPlants" id="KQK12482">
        <property type="protein sequence ID" value="KQK12482"/>
        <property type="gene ID" value="BRADI_1g03980v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840771"/>
    <dbReference type="Gramene" id="KQK12482">
        <property type="protein sequence ID" value="KQK12482"/>
        <property type="gene ID" value="BRADI_1g03980v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840771"/>
    <dbReference type="eggNOG" id="KOG1304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009646_6_0_1"/>
    <dbReference type="OMA" id="NQMKNPQ"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GLM6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF349">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="21"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK12482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="439" mass="45778" checksum="0F0BF9E12BD75D76" modified="2012-06-13" version="1">MAPECCKAPLLEGRGTKGCATPAQTLGNIVVSIVGTGVLGLPYAFRTAGWLAGSLGVAGAGAATFYCMLLLLDCRDKLREEELEEGQRQGQQDEERRHGSYTYGDLGERCFGPIGRYFTEAIIILCQTGGTVAYLVFIGQNISSVFPGSVRVSPATVVLAFLLPAEVALSFVRSLSALAPFSILADACTALAVAAVVKEDLALLAGQSAFDGGRSAFAGLWGVPFACGVAVFCFEGFCLTLALEASMADRARFRPVLLQAIAGVSAVYVGFGVCGYLAYGDATKDIVTLNLPSTWSTAAIKVVLCVALALTFAVMMHPIHEIVEARLFGAGGWWARRRGDTAGAGARGDAVERAALQLSRVAVVTALAGVACFVPAFGEFAAFVGSTVCALLSFVLPALFHLRVVGPAAGAWARAVDCGFLIFGVLFAAHGLYTAFSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRV5</accession>
    <name>I1GRV5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 6</fullName>
            <shortName evidence="5">eIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100846580</name>
        <name evidence="5" type="synonym">EIF6</name>
        <name evidence="6" type="ORF">BRADI_1g19960v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May also be involved in ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <text evidence="5">Shuttles between cytoplasm and nucleus/nucleolus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562588.1">
        <property type="nucleotide sequence ID" value="XM_003562540.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRV5"/>
    <dbReference type="STRING" id="15368.I1GRV5"/>
    <dbReference type="EnsemblPlants" id="KQK14985">
        <property type="protein sequence ID" value="KQK14985"/>
        <property type="gene ID" value="BRADI_1g19960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846580"/>
    <dbReference type="Gramene" id="KQK14985">
        <property type="protein sequence ID" value="KQK14985"/>
        <property type="gene ID" value="BRADI_1g19960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846580"/>
    <dbReference type="eggNOG" id="KOG3185">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071894_0_0_1"/>
    <dbReference type="OMA" id="WCAFCGM"/>
    <dbReference type="OrthoDB" id="4155914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK14985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="245" mass="26528" checksum="27949493353758CB" modified="2012-06-13" version="1">MATRIQFENNCEVGVFSKLTNAYCLVAIGGSENFYSTFESELADVIPVVKTSIGGTRIIGRLCVGNKNGLLLPHTTTDQELQHLRNCLPDQVVVQRIDERLSALGNCISCNDHVALTHPDLDKATEELIADVLGVEVFRQTIAGNILVGSYCTFSNRGGLVHPHTSIEDLDELSTLLQVPLVAGTVNRGSEVIAAGMTVNDWTAFCGSDTTATELSVIESVFKLREGQPTAIVDDMRKSLIDSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSR9</accession>
    <name>I1GSR9_BRADI</name>
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        <recommendedName>
            <fullName evidence="6">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100823134</name>
        <name evidence="7" type="ORF">BRADI_1g22700v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cystatin family. Phytocystatin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562767.1">
        <property type="nucleotide sequence ID" value="XM_003562719.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSR9"/>
    <dbReference type="MEROPS" id="I55.001"/>
    <dbReference type="EnsemblPlants" id="KQK15427">
        <property type="protein sequence ID" value="KQK15427"/>
        <property type="gene ID" value="BRADI_1g22700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823134"/>
    <dbReference type="Gramene" id="KQK15427">
        <property type="protein sequence ID" value="KQK15427"/>
        <property type="gene ID" value="BRADI_1g22700v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823134"/>
    <dbReference type="eggNOG" id="ENOG502S46Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113093_2_2_1"/>
    <dbReference type="OMA" id="KFAITEH"/>
    <dbReference type="OrthoDB" id="2016588at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027214">
        <property type="entry name" value="Cystatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47116">
        <property type="entry name" value="PHLOEM FILAMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982598" description="Cystatin domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="6">
        <location>
            <begin position="36"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK15427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="128" mass="13507" checksum="760B76300177E835" modified="2012-06-13" version="1">MARAFLLLAVLAAAVAAGASAAGIGGGGGGRRGHGTLFGGWRPIPDVSEPRIQELGGWAVAQHARLATDRLRFLRVTRGEQQVVAGMNYRLFVDAQDAAGRSAPYVAVVYEQSWTNTRKLTSFDPAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYI7</accession>
    <name>I1GYI7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100824366</name>
        <name evidence="13" type="ORF">BRADI_1g41840v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563854.1">
        <property type="nucleotide sequence ID" value="XM_003563806.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYI7"/>
    <dbReference type="STRING" id="15368.I1GYI7"/>
    <dbReference type="EnsemblPlants" id="KQK18343">
        <property type="protein sequence ID" value="KQK18343"/>
        <property type="gene ID" value="BRADI_1g41840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824366"/>
    <dbReference type="Gramene" id="KQK18343">
        <property type="protein sequence ID" value="KQK18343"/>
        <property type="gene ID" value="BRADI_1g41840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824366"/>
    <dbReference type="eggNOG" id="KOG1962">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109152_0_0_1"/>
    <dbReference type="OMA" id="MEMAVIL"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000011">
        <property type="entry name" value="B-cell receptor-associated protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF18">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="112"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK18343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="226" mass="24818" checksum="29FA11F15ADABFF6" modified="2012-06-13" version="1">MIQLLFTVLGAEAGLAAALLFKTPLRKLAMLALDRLKRGKGPVMVRTVAATVLVVLASSLHSMAKIHGHASGGELDAPGALTPTDQVLLARHLLEASLMGYSLFLALIIDRLHRYIREMRGLKKNLEAVSKQNKTLEEVKLVTSDGSKPYQKDIASLNEEIKKLRLQLKEKADEVKDAEARAVAAQTQSEGLMLKYDRLLEDKKHLHDQLQSGDIALSHSDGKKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1H245</accession>
    <name>I1H245_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U-box domain-containing protein 12</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Plant U-box protein 12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">RING-type E3 ubiquitin transferase PUB12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100826371</name>
        <name evidence="12" type="ORF">BRADI_1g52390v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557288.1">
        <property type="nucleotide sequence ID" value="XM_003557240.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H245"/>
    <dbReference type="FunCoup" id="I1H245">
        <property type="interactions" value="1967"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H245"/>
    <dbReference type="EnsemblPlants" id="KQK20089">
        <property type="protein sequence ID" value="KQK20089"/>
        <property type="gene ID" value="BRADI_1g52390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826371"/>
    <dbReference type="Gramene" id="KQK20089">
        <property type="protein sequence ID" value="KQK20089"/>
        <property type="gene ID" value="BRADI_1g52390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826371"/>
    <dbReference type="eggNOG" id="KOG0167">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006348_5_1_1"/>
    <dbReference type="OMA" id="DSHAIPK"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070696">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000082">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000114">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057623">
        <property type="entry name" value="PUB12-19-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF111">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25368">
        <property type="entry name" value="PUB10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="11">
        <location>
            <begin position="230"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="368"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="409"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK20089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="607" mass="65528" checksum="173885EA4A1626D2" modified="2012-06-13" version="1">MPARVAAEIAALPEPRGPLRRLSADLARRVRLLAPLLDEPSSSPDSSSPSYPLLADALRAARDLLQDVHHGSKIYQAMRGGDGLLHRFAGVNRQIQVALDQLPYQTFDMPEEVQEQVALVHSQFKRAAARTDSPDTQLSRDLDAALSDKACDAELLTRISEKLQLETMADMKKESVALHEMVISSGGEPDGSLDQMSFLLKKLKDCVIAQAPASDTLGGRSSSVKHRSPIIPDEFRCPISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKMQVPLSHTSLTPNFVLKSLIAQWCEANGIELPKNKANCRDKKAVKSSDYDNAGLVSLMNRLRSGNQDEQRAAAGEIRLLAKRNVNNRICIAEAGAIPLLVNLLSSSDPRTQEHAVTALLNLSIHENNKASIVDSNAIPKIVEVLKTGSMEARENAAATLFSLSVVDENKVTIGAAGAIPPLINLLCDGSPRGKKDAATAIFNLCIYQGNKVRAVKAGIIIHLMNFLVDPTGGMLDEALTLLAILAGNPEGKAVITQSEPIPPLVEVIRTGSPRNRENAAAILWSLCSADSEQTMAARAAGGEDALKELSETGTDRAKRKASSILELMRQAQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2Y6</accession>
    <name>I1H2Y6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable D-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="6">1.1.99.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100825375</name>
        <name evidence="12" type="ORF">BRADI_1g55030v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:38295"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.39" evidence="6"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561332.1">
        <property type="nucleotide sequence ID" value="XM_003561284.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2Y6"/>
    <dbReference type="FunCoup" id="I1H2Y6">
        <property type="interactions" value="910"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H2Y6"/>
    <dbReference type="EnsemblPlants" id="KQK20516">
        <property type="protein sequence ID" value="KQK20516"/>
        <property type="gene ID" value="BRADI_1g55030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825375"/>
    <dbReference type="Gramene" id="KQK20516">
        <property type="protein sequence ID" value="KQK20516"/>
        <property type="gene ID" value="BRADI_1g55030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825375"/>
    <dbReference type="eggNOG" id="KOG1232">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_4_1_1"/>
    <dbReference type="OMA" id="YNEDWMR"/>
    <dbReference type="OrthoDB" id="5332616at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H2Y6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051990">
        <property type="term" value="F:(R)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2190:FF:000001">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000002">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000002">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000001">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051264">
        <property type="entry name" value="FAD-oxidored/transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716:SF1">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="11">
        <location>
            <begin position="188"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK20516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="621" mass="67515" checksum="869C4DF093259BBB" modified="2012-06-13" version="1">MAILPFLSSRTRHSVLRGPLREAGRRALLSPAAPTYVHSPARPAEHTPTLPSTEFSSTGHRRPPPLDWTGAGARMISSSVGISHTRYGSTNHTLNSHKRFYWVPRPQRPSYRPGAGVATNEAHQSVNDSFEVQRRTFGSAAEPIQRNPAYSVLNPDDVSYFKSILGNNGVVQDKDRVAVANVDWMGKYKGASQLLLLPKSTNEVSKILAYCDSRRLAVVPQGGNTGLVGGSVPVHDEVIVNLGGMDKIVSFDNVNGILTCEAGCVLENLSTFVENEGFIMPLDLGAKGSCQIGGNISTNAGGLRFIRYGSLHGNVLGLEVVLANGTVLDMLTTLRKDNTGYDLKHLFVGSEGSLGIVTKVAILTPAKLPSTNVAFLSCNDYMSCQKLLLAARRSLGEIISAFEFMDRQCIDLAMTHLEGVHNPLPNLPYKFYVLIETTGSDESYDKAKLEAFLLRSMEDGLVTDGVIAQDISQASNFWRIREGISEASVKVGAVYKYDLSIPVEKLYDIVEEMRSRVGDMAEVLGYGHLGDGNLHLNILSSKYSDNILAQIEPFVYEWTAGHRGSISAEHGLGLMKAEKIHYSKSPEAVQLMASIKKLIDPNSILNPYKVLPRSVSAAGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I1H456</accession>
    <name>I1H456_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g58685v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H456"/>
    <dbReference type="STRING" id="15368.I1H456"/>
    <dbReference type="EnsemblPlants" id="KQK21093">
        <property type="protein sequence ID" value="KQK21093"/>
        <property type="gene ID" value="BRADI_1g58685v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21093">
        <property type="protein sequence ID" value="KQK21093"/>
        <property type="gene ID" value="BRADI_1g58685v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2944868_0_0_1"/>
    <dbReference type="InParanoid" id="I1H456"/>
    <dbReference type="OrthoDB" id="9948461at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK21093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="60" mass="7006" checksum="637A37082CCEA866" modified="2012-06-13" version="1">MKGLMILLEVHIMSPQRFYTHNIIWKQTLSIGVIIYILLCGSRPFWAQTEYGIFCICVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1H8B5</accession>
    <name>I1H8B5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100844310</name>
        <name evidence="8" type="ORF">BRADI_1g70600v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GOT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558530.1">
        <property type="nucleotide sequence ID" value="XM_003558482.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H8B5"/>
    <dbReference type="STRING" id="15368.I1H8B5"/>
    <dbReference type="EnsemblPlants" id="KQK22999">
        <property type="protein sequence ID" value="KQK22999"/>
        <property type="gene ID" value="BRADI_1g70600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844310"/>
    <dbReference type="Gramene" id="KQK22999">
        <property type="protein sequence ID" value="KQK22999"/>
        <property type="gene ID" value="BRADI_1g70600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844310"/>
    <dbReference type="eggNOG" id="KOG1743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124519_1_0_1"/>
    <dbReference type="OMA" id="MWLTDAQ"/>
    <dbReference type="OrthoDB" id="204784at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF253">
        <property type="entry name" value="GOLGI TRANSPORT 1 PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK22999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="140" mass="15799" checksum="7C669F2899CFA7D0" modified="2012-06-13" version="1">MVSFEMNDLKKIGLGLTGFGIFFSFLGIVFFFDKGLIAMGNILFLSGLGLTIGLKSTMQFFTKPKNYKGTISFGVGFFLVLIGWPFFGMLLEAYGFVVLFSGFWPTLAVFLQRIPILGWIFQQPFVTSFLDRYRGKRVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAE2</accession>
    <name>I1HAE2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Peroxidase</fullName>
            <ecNumber evidence="18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC100843299</name>
        <name evidence="21" type="ORF">BRADI_1g77130v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="18"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558948.1">
        <property type="nucleotide sequence ID" value="XM_003558900.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAE2"/>
    <dbReference type="FunCoup" id="I1HAE2">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HAE2"/>
    <dbReference type="EnsemblPlants" id="KQK23930">
        <property type="protein sequence ID" value="KQK23930"/>
        <property type="gene ID" value="BRADI_1g77130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843299"/>
    <dbReference type="Gramene" id="KQK23930">
        <property type="protein sequence ID" value="KQK23930"/>
        <property type="gene ID" value="BRADI_1g77130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843299"/>
    <dbReference type="eggNOG" id="ENOG502QPX7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010543_0_3_1"/>
    <dbReference type="OMA" id="CGMAEFI"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000007">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 18" id="KW-0349">Heme</keyword>
    <keyword evidence="12 18" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="9 15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="5 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 18" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982671" description="Peroxidase" evidence="18">
        <location>
            <begin position="23"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="28"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="38"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="71"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="126"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="205"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQK23930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="338" mass="34774" checksum="BDF5A4F74179445F" modified="2012-06-13" version="1">MAVLVLAAAVLWLCCAGRDAQAQAPATQLQLGFYAQSCPQAEVIVRDEVGRAVSGDPGLAAGLLRLHFHDCFVKGCDASVLLDTIAGNGSTAAEKDAAPNRTLRGFEVIDGAKKRLESACAGTVSCADILAFAARDSVVLTGGSPYGVPAGRRDGNESSASDAQASLPPPTANVAQLTQIFARNGLSQEDMVTLSGAHTIGVTHCSSFSARLYSGDNNNSDNTGHDPAMDDATATELARRCPPGSADTVPMDLGGGGGPVDENAFDTGYFQALLAHRGLLGSDQALTADNATAALVAQNAGNLYLFVTRFADAMVRMGAVRVLTGSDGQIRTSCRVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCR1</accession>
    <name>I1HCR1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100830918</name>
        <name evidence="8" type="ORF">BRADI_2g05320v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003565392.4"/>
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        <property type="gene ID" value="BRADI_2g05320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830918"/>
    <dbReference type="Gramene" id="KQK03068">
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        <property type="gene ID" value="BRADI_2g05320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830918"/>
    <dbReference type="eggNOG" id="KOG0955">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029005_0_0_1"/>
    <dbReference type="OMA" id="CIEYQEA"/>
    <dbReference type="OrthoDB" id="20839at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15571">
        <property type="entry name" value="ePHD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHD_BRPF_JADE_like"/>
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    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="Histone_Mod_Regulator"/>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING_FYVE_PHD ZINC FINGER SUPERFAMILY PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="399"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="465"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="261"/>
            <end position="291"/>
        </location>
    </feature>
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        <location>
            <begin position="306"/>
            <end position="331"/>
        </location>
    </feature>
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        <location>
            <begin position="368"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="316"/>
            <end position="329"/>
        </location>
    </feature>
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        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="596" mass="62941" checksum="11799F8B7916937B" modified="2012-06-13" version="1">MEIAADAAASGGCRIRDLPPSKRFKFVGSSRLGPAPCVPLPETAPLPVCLPAKKRAYAPAVAEVALKACLPAKKRAYAPLPDDIVPACLPAKKRVYVPPPPPLEDAAAHPPVPAKKRVHPLLPPGGAAAAPSSTKKRVHMPLPSLPENSAVSPPIPVRKRVQPVLPPRGATAAPSASAKKRVHVPLPLPPADAAASSSIPAKKKVHPPEAIAAPLSIPSKKLAQTPSPAEDDSSLAPVSLQANKRVMSPFICPSPCPPVESDGARVAAVKQPRSQRSVKRGGATDPRAAKGAVDCTRAEAFKVPANKPVKPKEVQEQASIKSGRASTAKESSDLHRKKLCDVVNGRQSEVQAEVLEKFEQAIDPQVVAPAREEEPKKEAEEVATEQKQEAAGEEDEEDGVLCAVCGSTDGDPSDPIVFCDGCDLMVHASCYGSPLAQSIPEGDWFCSLCSDKALATAKKGGKPPRPHCCLCPARGGAMKRTTDGAWAHIACALLVPEVFFQDPDGRDGIDCSRVPGHRYTKRCYICESSRGCALECSQPKCGLGFHVSCGLKGGLCIEYREEKAGAVVAGFCREHTKLWDKQQLTGKYKIVSRGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HEX2</accession>
    <name>I1HEX2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fungal lipase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100835215</name>
        <name evidence="5" type="ORF">BRADI_2g11800v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567598.1">
        <property type="nucleotide sequence ID" value="XM_003567550.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HEX2"/>
    <dbReference type="FunCoup" id="I1HEX2">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HEX2"/>
    <dbReference type="EnsemblPlants" id="KQK04122">
        <property type="protein sequence ID" value="KQK04122"/>
        <property type="gene ID" value="BRADI_2g11800v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835215"/>
    <dbReference type="Gramene" id="KQK04122">
        <property type="protein sequence ID" value="KQK04122"/>
        <property type="gene ID" value="BRADI_2g11800v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835215"/>
    <dbReference type="eggNOG" id="KOG2088">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020385_2_0_1"/>
    <dbReference type="OMA" id="EGEMWFE"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HEX2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005592">
        <property type="entry name" value="Mono/diacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023:SF6">
        <property type="entry name" value="LIPASE CLASS 3 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023">
        <property type="entry name" value="LIPASE CLASS 3 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03893">
        <property type="entry name" value="Lipase3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094580" description="Fungal lipase-like domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Mono-/di-acylglycerol lipase N-terminal" evidence="4">
        <location>
            <begin position="49"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="3">
        <location>
            <begin position="181"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK04122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="69444" checksum="076F4C6E78B9C4DD" modified="2012-06-13" version="1">MGTATMATALGAAMLLYFVLSRRLAHHEDSAGSPGGGGGVKRRRGRAARRPAQPPATWIEAVGTLAETLRFTYSETLGKWPIGDLAFGIKYLMRRQGNLHVASVYAGSNCTELKGHEVMEELIVLRRLIDLCFLFSKKSFPVFLELAGFSQEDVLIEEPKAGILKPAHTILRDECTKSFLVLIRGTHSMKDTLTAATGAVVPFHHSLLDEGGVSKLVLGYAHCGMVAAARWIARGITPCLLQAVSQCPEYQIKIVGHSLGGGTAALLTYILREHTEFSATTCVAFAPASCMTWELAESGKHFVTTIVNGADLVPTVSTASIDDLRSEVTASSWLNDLRDQIQQTRFLNVVYRSATALGTRLQSFSGARARVAGAGALLRPVSSKTLVVMKQAQNVAQAVARSRSALSSWSCMGARRRAVGVVAATTNDEMTAETHVTTTVDSDSFVVEQCGTKVVEELQYTATSISVHEETEEEALLSEHETSREHAEEITDGELWFEFEKDRDRQAEVEAQTREEEAAAAKEIMEEESAVLKNVEDRQSFSSDSLEGQQFYPPGRIMHMVAMPPPDAGPDDPVVTDECTVGIYQTPRDLYSKIRLSNTMINDHYMPMYKKMMEILIENFANNEDNFCADSTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJ17</accession>
    <name>I1HJ17_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100836638</name>
        <name evidence="9" type="ORF">BRADI_2g24130v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568374.1">
        <property type="nucleotide sequence ID" value="XM_003568326.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJ17"/>
    <dbReference type="STRING" id="15368.I1HJ17"/>
    <dbReference type="EnsemblPlants" id="KQK06057">
        <property type="protein sequence ID" value="KQK06057"/>
        <property type="gene ID" value="BRADI_2g24130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836638"/>
    <dbReference type="Gramene" id="KQK06057">
        <property type="protein sequence ID" value="KQK06057"/>
        <property type="gene ID" value="BRADI_2g24130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836638"/>
    <dbReference type="eggNOG" id="KOG2946">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059592_2_1_1"/>
    <dbReference type="OMA" id="FVAMMMA"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HJ17">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF20">
        <property type="entry name" value="PROTEIN YIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="140"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK06057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="279" mass="29700" checksum="B1797D7A4BD3739C" modified="2012-06-13" version="1">MSHPHPQGDTVPLHPSSAQSDMDEIESLIHAAPTAAAVLPARPSSPPRASIPISNSPPVAAPTSSKPQPVPPFSSAVSSSSAPPRSASVTIPIAADGFGAPPNTLTEPVWDTVQRDLSRIVSNLKLVVFPNPFREDPGKALRDWDLWGPFFFIVFLGLTLSWSASVKKSEVFAVAFAVLAAGAIILTLNVLLLGGHIIFFQSLSLLGYCLFPLDVGALICLMKDNVVLKIVVVAVTIAWSSWAAYPLMSTAVNPRRKALALYPVFLMYVSVGFLIIAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJV2</accession>
    <name>I1HJV2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Membrane-associated kinase regulator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100835220</name>
        <name evidence="2" type="ORF">BRADI_2g26620v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568537.1">
        <property type="nucleotide sequence ID" value="XM_003568489.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJV2"/>
    <dbReference type="STRING" id="15368.I1HJV2"/>
    <dbReference type="EnsemblPlants" id="KQK06489">
        <property type="protein sequence ID" value="KQK06489"/>
        <property type="gene ID" value="BRADI_2g26620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835220"/>
    <dbReference type="Gramene" id="KQK06489">
        <property type="protein sequence ID" value="KQK06489"/>
        <property type="gene ID" value="BRADI_2g26620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835220"/>
    <dbReference type="eggNOG" id="ENOG502QVKJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_765951_0_0_1"/>
    <dbReference type="OMA" id="MYCGDTS"/>
    <dbReference type="OrthoDB" id="1927169at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019210">
        <property type="term" value="F:kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039620">
        <property type="entry name" value="BKI1/MAKR1/3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33312:SF8">
        <property type="entry name" value="MEMBRANE-ASSOCIATED KINASE REGULATOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33312">
        <property type="entry name" value="MEMBRANE-ASSOCIATED KINASE REGULATOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="40266" checksum="15CEC4FA2ECDBB25" modified="2012-06-13" version="1">MGRSRAKDGAVKSSFPSPASSSSASSSEFEFTVTQSPASKQRSAAQLCPADDLFYKGQLLPLHLSPRISMVRTLLLASASTSSASASDSTSRDSNGSTSSSFSADCASADDLLLLPDSAPSSSRPSSAAADVDGRHFNHHLLRGTSDAAAASFPPAASRRSGKQYLSSFATRFSSVFLHRGGAPAPASGKKPSSSNKSLAKEVIKKYAKKVKPLYEKLSQIPKNQSSHPPQPQPQQQGFKKPFSFSIRKKRGDEDHAALVTPSMAADQAGGGNGKYAHSNSFSGNLRFPRQKRCAASCPSSMRSSPNHSGLLSFGGAGGVGFPDVPAAAAAAMAGGIAGGRPAVSLSTASSSSMEELQSAIEGAIAHCKYSMGGVVSLCPRKGAAGEICAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HLS4</accession>
    <name>I1HLS4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transport inhibitor response 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100822026</name>
        <name evidence="9" type="ORF">BRADI_2g35720v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of a SCF (SKP1-cullin-F-box) protein ligase complex. May interact with auxin and auxin-responsive proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568922.1">
        <property type="nucleotide sequence ID" value="XM_003568874.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HLS4"/>
    <dbReference type="FunCoup" id="I1HLS4">
        <property type="interactions" value="446"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HLS4"/>
    <dbReference type="EnsemblPlants" id="KQK07484">
        <property type="protein sequence ID" value="KQK07484"/>
        <property type="gene ID" value="BRADI_2g35720v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822026"/>
    <dbReference type="Gramene" id="KQK07484">
        <property type="protein sequence ID" value="KQK07484"/>
        <property type="gene ID" value="BRADI_2g35720v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822026"/>
    <dbReference type="eggNOG" id="KOG1947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022456_1_0_1"/>
    <dbReference type="OMA" id="TDDCLEM"/>
    <dbReference type="OrthoDB" id="423607at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000029">
        <property type="entry name" value="Transport inhibitor response 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041567">
        <property type="entry name" value="COI1_F-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041101">
        <property type="entry name" value="Transp_inhibit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134">
        <property type="entry name" value="F-BOX/TPR REPEAT PROTEIN POF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134:SF66">
        <property type="entry name" value="PROTEIN TRANSPORT INHIBITOR RESPONSE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18511">
        <property type="entry name" value="F-box_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18791">
        <property type="entry name" value="Transp_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="COI1 F-box" evidence="7">
        <location>
            <begin position="20"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Transport inhibitor response 1" evidence="8">
        <location>
            <begin position="78"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK07484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="590" mass="64846" checksum="EF7DFF3BC02EE82B" modified="2012-06-13" version="1">MGRGVGSRSGGGAAQPPWHSLPDEVWEHAFSFLPAAADRGAAAGACRGWLRAERRSRRRLAVANCYATSPRDAVERFPSVRAAEVKGKPHFADFGLVPPAWGAEAAPWVAAAADGWPLLEELSFKRMVVTDECLEMIAASFRNFQVLRLVSCEGFSTAGLAAITEGCRNLRELDLQENYIEDCSSHWLSSFPESFTSLETLNFSCLDGEVNFAVLERLVTRCHNLKTLKLNNAIPLDKVASLLRKAPHIVELGTGKFSADYHPDLFAKLEAAFAGCKSLRRLSGAWDAVPDYLSAFYGVCEGLTSLNLSYATVRGPELIKFISRCKNLQQLWVMDLIEDHGLAVVASTCSKLQELRVFPSDPFGAGQVLLTERGLVDVSASCPMLESVLYFCRRMTNEALITIAKNRPNFTCFRLCILEPRTPDYITQQSLDAGFSAIVESCKGLRRLSVSGLLTDLVFKSIGEHADRLEMLSIAFAGNSDLGLHYILSGCKSLKKLEIRDCPFGNKPLLANAAKLETMRSLWMSSCSLTLGACRQLAQKMPRLTVEIMNDPGRACPLDALPDESPVEKLYVYRTIAGPRSDTPDYVQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPH2</accession>
    <name>I1HPH2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100846907</name>
        <name evidence="4" type="ORF">BRADI_2g43760v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566893.1">
        <property type="nucleotide sequence ID" value="XM_003566845.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPH2"/>
    <dbReference type="STRING" id="15368.I1HPH2"/>
    <dbReference type="EnsemblPlants" id="KQK08768">
        <property type="protein sequence ID" value="KQK08768"/>
        <property type="gene ID" value="BRADI_2g43760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846907"/>
    <dbReference type="Gramene" id="KQK08768">
        <property type="protein sequence ID" value="KQK08768"/>
        <property type="gene ID" value="BRADI_2g43760v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846907"/>
    <dbReference type="eggNOG" id="KOG1190">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033500_0_0_1"/>
    <dbReference type="OMA" id="FCDTETA"/>
    <dbReference type="OrthoDB" id="296632at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12686">
        <property type="entry name" value="RRM1_PTBPH1_PTBPH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000324">
        <property type="entry name" value="Polypyrimidine tract-binding protein-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034792">
        <property type="entry name" value="PTBPH1/PTBPH2_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="17"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="237"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="524"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK08768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="544" mass="58468" checksum="BEA7878F520DFB92" modified="2012-06-13" version="1">MASGGNPQFRYTQPPSKVIHVRNLPWECTDEELAELGSPFGKVVNTKCNVGANRNQAFIEFADQNQAIAMISYYASSAEPAQVRGKNVYLQYSNRQEIVNSKSTGDAAGNVLLVTMEGVLPDAVSIDVLHLVFSAFGYVHKIATFEKASGYQALIQFSDAETASSAKAALDGRCIPSYLLPDLDGACTLRINYSAHSVLNVKFQSHRSRDYTNPYLPLAPSAIDGSGLDGKKQEAESNVLLASVENMQYIVTIDALHEVFSAFGFVHKIAIFEKNSGFHALIQYPDIQTAVKAREALEGHSIYEGGYCKLHLTFSRHTDLNVRINNERGRDYTGGSSAPANYEPSILGPQPTLPVGAVAPPYSSASPAATGAVVPPGTTTILATPSPRPFHPSGEPHPQSAGGPQQYPNQGILQGALGVPPQIPGYGAPEFPQGPSQAQMAHHSGQGSQQMPNHQLPQGMPFPGHGGQQLHPGPQMMQAPGYRGPPFPQGPMQPMPQFPVYGNQQFPVYGNQQFPPGSGPQMMGFPGQGGQYPPFGRPLHPYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPU6</accession>
    <name>I1HPU6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">protein-S-isoprenylcysteine alpha-carbonyl methylesterase</fullName>
            <ecNumber evidence="10">3.1.1.n2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BRADI_2g44942v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the demethylation of isoprenylcysteine methylesters.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + H2O = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48520"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="3.1.1.n2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the AB hydrolase superfamily. Isoprenylcysteine methylesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.n2" evidence="10"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPU6"/>
    <dbReference type="STRING" id="15368.I1HPU6"/>
    <dbReference type="ESTHER" id="bradi-i1hpu6">
        <property type="family name" value="BD-FAE"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72475">
        <property type="protein sequence ID" value="PNT72475"/>
        <property type="gene ID" value="BRADI_2g44942v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72475">
        <property type="protein sequence ID" value="PNT72475"/>
        <property type="gene ID" value="BRADI_2g44942v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1516">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_2_4_1"/>
    <dbReference type="InParanoid" id="I1HPU6"/>
    <dbReference type="OMA" id="STYCLLA"/>
    <dbReference type="OrthoDB" id="6495301at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010296">
        <property type="term" value="F:prenylcysteine methylesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000084">
        <property type="entry name" value="Isoprenylcysteine alpha-carbonyl methylesterase ICME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049492">
        <property type="entry name" value="BD-FAE-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF33">
        <property type="entry name" value="KYNURENINE FORMAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20434">
        <property type="entry name" value="BD-FAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="BD-FAE-like" evidence="15">
        <location>
            <begin position="127"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="PNT72475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="409" mass="45184" checksum="4EDBB3BD420C07D7" modified="2012-06-13" version="1">MQPPSSGGDAREADALLARSDSAGRRRRASPVQSASPAPQGWGGPRRQSSFRDDVGHAASETYLVTRLTFTLLQYLGLGYRWMTQLLALAVYAILLMPGFLQVGYYYFFSSQVRRSIVYGEQPRNRLDLYIPKDNTRPCPVMAFVTGGAWIIGYKAWGALLGRRLAERGIIVACIDYRNFPQGTISDMVADASQGISFVCNNIASYGGDPNQIYLMGQSAGAHIAACALMEQAVKESSGQPISWSVTQIKAYFGLSGGYNIYNLVDHFHQRGLYRSIFLSIMDGEESLSRYSPEIVVKESSPQTIALLPPIVLMHGTDDYSIPSSASQTFVDVLQQVGAQARLLLYEGKTHTDIFLQDPLRGGKDPLVEDVLSVIHTDDATRRNIASAPTPRRLVFEWQLQLARRISPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1HR60</accession>
    <name>I1HR60_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g48780v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HR60"/>
    <dbReference type="EnsemblPlants" id="KQK09564">
        <property type="protein sequence ID" value="KQK09564"/>
        <property type="gene ID" value="BRADI_2g48780v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09564">
        <property type="protein sequence ID" value="KQK09564"/>
        <property type="gene ID" value="BRADI_2g48780v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008602_1_0_1"/>
    <dbReference type="InParanoid" id="I1HR60"/>
    <dbReference type="OMA" id="CTPASIG"/>
    <dbReference type="OrthoDB" id="678085at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HR60">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36892:SF7">
        <property type="entry name" value="HVA22-LIKE PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36892">
        <property type="entry name" value="OS01G0201800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK09564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1009" mass="109959" checksum="62CB00ECD2789C43" modified="2012-06-13" version="1">MAGAFAAASAAEPFSIRGFAARMRAVDAAKSCPFGGCGNGESVLPPQQLPPMDPPPRSRWWAHELSAARARSGFAAGGEEAAMGSGEGDSLRKAMKRKGSRGSGGAERAKKRQKALQFSLFLKHKERTSKPQSASCSLQNVFYKGLRKHTSCTTRRLRELAVRKKLQERQDHTLTCGNSLKKQCVRGMDHKLRIKVSRPINYPPNSGCEVVKHVAYPPKDDIFGDLPLLESSKIMFHTGVDILPTVIEDPFAVNQSVPDAILGTEPLKLMPASEISKQTPLPFEDFVKKEGTPEKETTCISPNDVGKGHSSNAEFDGLLNHGSDTIGKTYLGEMQMKSTNVSALSSYSNEGAKSCSNNNSPRSCFYTNTNCFQRIKKTGTSSAAVRTRIEETKNDRDAAVIVKTSNCISGRQVPNEYHLSSEGSVLSSAISQGAKSCSSNPPQSCFYMGTNCVRTIKKTGTSSAAVRTRIEATKNNRDAAVIAKKRNYISGRQVPTEYHLSSEGSLVSSAISQGTVNAGTNTDGLSSCRSVPAQEYVSTSSPFYKFASNACDESRKSVDTCASSSVKDQGSLYPKHQPAYPSASIGLAFMKLPGLERMEISSHDPRTGENKFMNGLSLNTRRCQEQQMASGMTNIIQGQNMIGFSDSQAGKKVPDCFVRQDNFHSHQPTMRLMGKTVSVCERVKEHKLSTTGKVWIDSSITEEDRPSPVSCQLPQKRLCPYPVSVIPRACVNESSYIFSSISNSTLAEARPIASDAQNQRLQLINSVSSTVKDHISNPISHFVCQAQINKESVIDVNSRTRHLELYQPQKLMNVPQNQYPHLNTPASSMYMEDHTSLGSAVNHSSPSFPQRVLNTSMQGKYQKCTSLGYEDTSSVPTHKHCQVPGANLLSTSIVSFREYGTNSAELRNSYQAGRPSVTTSSSSKSIPTISPTCTGSLFNIDGRKGVSFINQSNKRPACADNVLYQPAKRQLVADKLELTTPMVPNIQNYSLGWSLNDAVGPRILDFSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HTS9</accession>
    <name>I1HTS9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100842337</name>
        <name evidence="8" type="ORF">BRADI_2g56250v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232619.1">
        <property type="nucleotide sequence ID" value="XM_010234317.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HTS9"/>
    <dbReference type="FunCoup" id="I1HTS9">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK10795">
        <property type="protein sequence ID" value="KQK10795"/>
        <property type="gene ID" value="BRADI_2g56250v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK10796">
        <property type="protein sequence ID" value="KQK10796"/>
        <property type="gene ID" value="BRADI_2g56250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842337"/>
    <dbReference type="Gramene" id="KQK10795">
        <property type="protein sequence ID" value="KQK10795"/>
        <property type="gene ID" value="BRADI_2g56250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10796">
        <property type="protein sequence ID" value="KQK10796"/>
        <property type="gene ID" value="BRADI_2g56250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842337"/>
    <dbReference type="eggNOG" id="KOG4474">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1013267_0_0_1"/>
    <dbReference type="OMA" id="WPWAFEA"/>
    <dbReference type="OrthoDB" id="10266980at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HTS9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071709">
        <property type="term" value="P:membrane assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097035">
        <property type="term" value="P:regulation of membrane lipid distribution"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF4">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="62"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK10795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="276" mass="31093" checksum="AC1A1ED67BF2E977" modified="2012-06-13" version="1">MPPRRAGAGGGDTTAFFAATLVLWAVSVGFEIGARGRRELAAVAAGFAFFQAANTAVRAAVSRDPLFVNTAVSLLHSSLTSASVVFVLLCRWRKKGLENMFEHEELVGSSWVGAYSALCFSCGYFAYDQLDMLRYRLYSGWIPGILMHHLILLICFTLALYRNVTINYLILSLVCEMHSIFLHIRKVRRMAGFRDFNRKMVKLEWILNWSTFLTARVICHILITYKLITDAHKFDKGIELPLALFGMAGMNVLNISLGLDLFKAFARERNQQVHQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1HU55</accession>
    <name>I1HU55_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC100828381</name>
        <name evidence="24" type="ORF">BRADI_2g57410v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">Bd21</strain>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567350.1">
        <property type="nucleotide sequence ID" value="XM_003567302.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HU55"/>
    <dbReference type="EnsemblPlants" id="KQK10982">
        <property type="protein sequence ID" value="KQK10982"/>
        <property type="gene ID" value="BRADI_2g57410v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73355">
        <property type="protein sequence ID" value="PNT73355"/>
        <property type="gene ID" value="BRADI_2g57410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828381"/>
    <dbReference type="Gramene" id="KQK10982">
        <property type="protein sequence ID" value="KQK10982"/>
        <property type="gene ID" value="BRADI_2g57410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73355">
        <property type="protein sequence ID" value="PNT73355"/>
        <property type="gene ID" value="BRADI_2g57410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828381"/>
    <dbReference type="eggNOG" id="ENOG502QUMK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_116_2_1"/>
    <dbReference type="OMA" id="GFHARQP"/>
    <dbReference type="OrthoDB" id="643280at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000227">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000250">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF19">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094810" description="Receptor-like serine/threonine-protein kinase" evidence="20">
        <location>
            <begin position="29"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="459"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="22">
        <location>
            <begin position="29"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="23">
        <location>
            <begin position="359"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="513"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="544"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="PNT73355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="824" mass="89407" checksum="7F620EB6FCF8540D" modified="2012-06-13" version="1">MARRRGRCSAAALWLLGFMLLRGAPSRAADTVAVGRPLSGRQTLVSKRGKFALGFFQPDNSRQNWYIGIWYNQISKHTPVWVANRNAPTSDPASSQLSISDDGNVVLVDKHDANKAPIWSTNLTNIASGSNTVGIILDTGNLVLADASNTSVVLWQSFDHFGDTWLPGGKLGRNNRTGEVTRLFAWKSFDDPATSVFSLELDPDGTSQYLLNWNGTREYWTSGTWNGHMFAAVPEMMASNASPMSLYTFDYVEGKEGSYFVYDVKDDDAAVVTRFVVDVTGQIKFLTWVESVGDWILFWSQPKAQCDVYALCGAFSACTEKSLPSCSCLRGFRERRLSAWMQGDHTAGCARDAELRQQCGGGVVHGAMPKGNNDGFYAMPGVKLPSDGQGVAAAASGGECEAACLAKCACTAYAYNGSCWLWHGGLINLQVQDTGSGGGTIMIRLAASEFSTTGHAKKLTIILVVVAAAVAVFSVLVLALVLRSRNRSRVRAARRVEGSLMAFTYRDMQSVTSNFSEKLGGGAFGSVFKGSLPDATATPVAVKKLEGVRQGEKQFRAEVSTIGTIQHVNLIRLLGFCSDRAHRLLVYEHMPNGSLDKHLFRSNDGHGIGSILSWKTRYRIALDIARGMEYLHEKCRDRIIHCDVKPENILLDGAFAAKVSDFGMAKLVGRGFSRVLTTMRGTVGYLAPEWITGAAVTAKADVFSYGMVLFEIVSGRRNVEQREDGGAVDFFPSMAVSRLHGGGEMKSVVDGRLGGEVDVDQVERACKVACWCVQDDEGARPSMGMVVQVLEGLVDVSVPPIPRSLRVLGDPPSYVKFFSGLPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXY5</accession>
    <name>I1HXY5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 16">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="12 16">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100821010</name>
        <name evidence="19" type="ORF">BRADI_3g06000v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">Bd21</strain>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Ubiquitin-protein hydrolase involved in the release of ubiquitin attached via both peptide and isopeptide linkages. Able to cleave 'Lys-48'-linked polyubiquitin chains. Involved in the direct or indirect regulation of AUX/IAA proteins stability. Acts as a linker between the TREX-2 complex and 26S proteasome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12 15 16">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="12 16"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570695.1">
        <property type="nucleotide sequence ID" value="XM_003570647.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXY5"/>
    <dbReference type="STRING" id="15368.I1HXY5"/>
    <dbReference type="MEROPS" id="C12.A01"/>
    <dbReference type="EnsemblPlants" id="KQJ93652">
        <property type="protein sequence ID" value="KQJ93652"/>
        <property type="gene ID" value="BRADI_3g06000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821010"/>
    <dbReference type="Gramene" id="KQJ93652">
        <property type="protein sequence ID" value="KQJ93652"/>
        <property type="gene ID" value="BRADI_3g06000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821010"/>
    <dbReference type="eggNOG" id="KOG2778">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018316_0_1_1"/>
    <dbReference type="OMA" id="PNLFFAK"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010016">
        <property type="term" value="P:shoot system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09617">
        <property type="entry name" value="Peptidase_C12_UCH37_BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.860:FF:000003">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000003">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017390">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041507">
        <property type="entry name" value="UCH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF30">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18031">
        <property type="entry name" value="UCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038120">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52049">
        <property type="entry name" value="ULD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 12 15" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12 15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 12 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="18">
        <location>
            <begin position="2"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="216"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="13 15">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13 15">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="15">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="14 15">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQJ93652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="327" mass="37293" checksum="05172B6BD81326C7" modified="2012-06-13" version="1">MSWAAVENDPGVFTELLQQMQLKGLQVDELYSLDLDALKDIQPVYGLIILYKWRPPEKDERPVIKDAVPNLFFANQIINSACATQALVSVLLNSSGITLSDDLRKLKEFAKDLPPELKGLAIVNCESIRVTSNSFARSEDSEEQKASSKDDDVYHFISYVPVDGVLYELDGLKDGPISLGKTPGGAGDIGWLRMVQPVIQERIDQFSQNEIRFSVMAITKNRKEMYIVELKELQRKRENLLSQMGDPSVNRQRQSVERSLAEVAAQIEAVTEKIIMEEEKSKKWKTENIRRKHNYVPFLFNFLKILEEKKQLKPLIEKAKQKSPNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZ54</accession>
    <name>I1HZ54_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 21">Peroxidase</fullName>
            <ecNumber evidence="4 21">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BRADI_3g09140v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 21">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 21">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="18 21">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 21">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="18 21">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 21"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZ54"/>
    <dbReference type="FunCoup" id="I1HZ54">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ94196">
        <property type="protein sequence ID" value="KQJ94196"/>
        <property type="gene ID" value="BRADI_3g09140v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ94196">
        <property type="protein sequence ID" value="KQJ94196"/>
        <property type="gene ID" value="BRADI_3g09140v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ2M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010543_0_1_1"/>
    <dbReference type="InParanoid" id="I1HZ54"/>
    <dbReference type="OMA" id="GYAECED"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000009">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF116">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 21" id="KW-0349">Heme</keyword>
    <keyword evidence="15 21" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="11 18 21" id="KW-0408">Iron</keyword>
    <keyword evidence="7 18 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 21" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="14" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982797" description="Peroxidase" evidence="21">
        <location>
            <begin position="24"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="22">
        <location>
            <begin position="24"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="18">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="19">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="34"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="67"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="122"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KQJ94196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="323" mass="35099" checksum="9D3D99E9D167A311" modified="2012-06-13" version="1">MAWSTTTWHCLVALSLLSSAAYGQLNTKFYDYSCPHLEFIVRLSMFKAILTERRMGASLLRLHFHDCFVQGCDGSILLDDVPGKNFTGEKTAFPNVNSVRGFEVIDDIKRNVEYFCPGVVSCADILALAAREGTVLLGGPSWAVPLGRRDSTTASLDAANNDLPPPTLNLSALIQSFANKSLSARDLTALSGAHTIGFSQCLNFRDHVYNDTNIDPAFATLRRGNCPAAAPNGDTNLAPFDVQTQLRFDNAYYGNLLAKRGLIHSDQELFNGASQDALVQQYSANQALFFADFAAAMIKMGNLSPLTGNAGQIRRNCRAVNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZC7</accession>
    <name>I1HZC7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100826874</name>
        <name evidence="6" type="ORF">BRADI_3g09770v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572201.1">
        <property type="nucleotide sequence ID" value="XM_003572153.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZC7"/>
    <dbReference type="FunCoup" id="I1HZC7">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HZC7"/>
    <dbReference type="EnsemblPlants" id="KQJ94306">
        <property type="protein sequence ID" value="KQJ94306"/>
        <property type="gene ID" value="BRADI_3g09770v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826874"/>
    <dbReference type="Gramene" id="KQJ94306">
        <property type="protein sequence ID" value="KQJ94306"/>
        <property type="gene ID" value="BRADI_3g09770v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826874"/>
    <dbReference type="eggNOG" id="ENOG502QT6M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024600_2_0_1"/>
    <dbReference type="OMA" id="RIIPMTP"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000008">
        <property type="entry name" value="BTB/POZ domain-containing protein POB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000106">
        <property type="entry name" value="BTB/POZ domain-containing protein POB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045890">
        <property type="entry name" value="POB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46336">
        <property type="entry name" value="OS02G0260700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46336:SF21">
        <property type="entry name" value="OS02G0260700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21536">
        <property type="entry name" value="BTB_KLHL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="136"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ94306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="552" mass="61989" checksum="3E4A72FF05838C8B" modified="2012-06-13" version="1">MAGRDASTVAGAAAEAEMDPDFSSGEGGPSFEFAFNSVNFSDRVLRIEVVGTDDAPGSRGDVAGGSLSDWARHRKRRREELRKEKECATHMSDQTNCNDVEAEECDAYEENQEEPVAMIEESPPSIGLDGDDGRSIDSWTVVSTPILRVNTIYISSAILAAKSQFFFKLFSNGMKESDQRHATLRITDSEENALMELLSFMYSGKLTTTEPTLLLDILMAADKFEVVSCMRHCSQLLTSLPMTPESALLYLDLPCSISMATAVQPLTDAAKEFLANRYKDLTKFQDEVMSIPLAGIEAILSSNDLQVASEDAIYDFLLRWARGQYPKSEERREILSSRLLPLVRFSHMTCRKLRKVLTCTDIDHEQATKCVTEALLYKADAPHRQRALAADTMTCRKFAERAYKYRPLKVVEFDRPYPQCIAYLDLKRDECSRLFPSGRIYSQAFHLAGQGFFLSAHCNMEQQSTFYCFGLFLGMQEKGSMSVTVDYEFAARTRPSGEFVSKYKGNYTFTGGKAVGYRNLFAIPWSTFMADDSLFFIDGMLHLRAELTIKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I1I4J6</accession>
    <name>I1I4J6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g28310v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I4J6"/>
    <dbReference type="FunCoup" id="I1I4J6">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ97017">
        <property type="protein sequence ID" value="KQJ97017"/>
        <property type="gene ID" value="BRADI_3g28310v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97017">
        <property type="protein sequence ID" value="KQJ97017"/>
        <property type="gene ID" value="BRADI_3g28310v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R4TG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132313_2_0_1"/>
    <dbReference type="InParanoid" id="I1I4J6"/>
    <dbReference type="OMA" id="MMEGKSQ"/>
    <dbReference type="OrthoDB" id="608266at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34538">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34538:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ97017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="86" mass="9861" checksum="EF092F3CF55F46B6" modified="2012-06-13" version="1">MAFLLGLLRVTKSVGSGRRRMDAKSGAGTVSSWRRTPAPMRQLFWRVRRAMLQRASRGGSVRFGYDLKSYTQNFDDGLVYVCRRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6G6</accession>
    <name>I1I6G6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NERD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100838016</name>
        <name evidence="2" type="ORF">BRADI_3g34290v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574386.1">
        <property type="nucleotide sequence ID" value="XM_003574338.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6G6"/>
    <dbReference type="STRING" id="15368.I1I6G6"/>
    <dbReference type="EnsemblPlants" id="KQJ97953">
        <property type="protein sequence ID" value="KQJ97953"/>
        <property type="gene ID" value="BRADI_3g34290v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67965">
        <property type="protein sequence ID" value="PNT67965"/>
        <property type="gene ID" value="BRADI_3g34290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838016"/>
    <dbReference type="Gramene" id="KQJ97953">
        <property type="protein sequence ID" value="KQJ97953"/>
        <property type="gene ID" value="BRADI_3g34290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67965">
        <property type="protein sequence ID" value="PNT67965"/>
        <property type="gene ID" value="BRADI_3g34290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838016"/>
    <dbReference type="eggNOG" id="ENOG502QQW6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074946_0_0_1"/>
    <dbReference type="OMA" id="NCRPSYA"/>
    <dbReference type="OrthoDB" id="1874403at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011528">
        <property type="entry name" value="NERD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35287">
        <property type="entry name" value="SI:ZFOS-911D5.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35287:SF1">
        <property type="entry name" value="SI:ZFOS-911D5.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08378">
        <property type="entry name" value="NERD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50965">
        <property type="entry name" value="NERD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NERD" evidence="1">
        <location>
            <begin position="34"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ97953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="325" mass="36673" checksum="A4387E251FF81C85" modified="2012-06-13" version="1">MWVEIFCGLVAYKLIRTVFFSDDGVDQLADLDSSHSDLCFAVASRLEKLYGGRCFVGLRIPDPDAGERQHIDVVLVTKREVMMVAVKNLSGFVEVDKDGNWSSEKKRKQEVLPNPVLEVKRAATNLQSYLEQRGAALPDELVNGRVLLPNPNCRPSYTINLQPEVISYDQWKDLKADTKGGLSTWIKGAFHGSKSDISDLLLQNLHSILSTSPMCDRLELKGDKNVLGEFMEFKGRHEDIQLLKKLKRSKVSKFIIQKSTLFGGFGRSRVQILYSPRDYRAEGTSSSEWKEISVKQYTEILFQPLHSKKVRKFKLSSVASVTLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1I7R2</accession>
    <name>I1I7R2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HVA22-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100828818</name>
        <name evidence="2" type="ORF">BRADI_3g37920v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574605.1">
        <property type="nucleotide sequence ID" value="XM_003574557.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I7R2"/>
    <dbReference type="EnsemblPlants" id="KQJ98595">
        <property type="protein sequence ID" value="KQJ98595"/>
        <property type="gene ID" value="BRADI_3g37920v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828818"/>
    <dbReference type="Gramene" id="KQJ98595">
        <property type="protein sequence ID" value="KQJ98595"/>
        <property type="gene ID" value="BRADI_3g37920v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828818"/>
    <dbReference type="eggNOG" id="KOG1725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098452_1_0_1"/>
    <dbReference type="OMA" id="DGRDHPQ"/>
    <dbReference type="OrthoDB" id="10009287at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF139">
        <property type="entry name" value="HVA22-LIKE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ98595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="130" mass="15004" checksum="5BB253AD3120D9C4" modified="2012-06-13" version="1">MGKSWALLTHLHSVAGPSITLLYPLYASVCAMESPSKVDDEQWLAYWILYSFITLLELVAEPVLYWIPVWYPVKLLFVAWLALPQFKGASFIYEKLVREQLRKYRGRARRGDADHKVHIAKAEANHGHVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1I9F7</accession>
    <name>I1I9F7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="4">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FAR2</name>
        <name evidence="9" type="synonym">LOC100824003</name>
        <name evidence="8" type="ORF">BRADI_3g42780v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>Three Fatty Acyl-Coenzyme A Reductases, BdFAR1, BdFAR2, and BdFAR3, are involved in primary alcohols biosynthesis of cuticular wax in Brachypodium distachyon.</title>
            <authorList>
                <person name="Wang Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="4"/>
    <dbReference type="EMBL" id="MF285085">
        <property type="protein sequence ID" value="ASK86470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574925.1">
        <property type="nucleotide sequence ID" value="XM_003574877.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I9F7"/>
    <dbReference type="STRING" id="15368.I1I9F7"/>
    <dbReference type="EnsemblPlants" id="KQJ99354">
        <property type="protein sequence ID" value="KQJ99354"/>
        <property type="gene ID" value="BRADI_3g42780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824003"/>
    <dbReference type="Gramene" id="KQJ99354">
        <property type="protein sequence ID" value="KQJ99354"/>
        <property type="gene ID" value="BRADI_3g42780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824003"/>
    <dbReference type="eggNOG" id="KOG1221">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="WRFFIAR"/>
    <dbReference type="OrthoDB" id="429813at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I9F7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010345">
        <property type="term" value="P:suberin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF39">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioester reductase (TE)" evidence="6">
        <location>
            <begin position="24"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="5">
        <location>
            <begin position="412"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASK86470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ99354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="510" mass="57361" checksum="E026444F9838309B" modified="2012-06-13" version="1">MVKMDGSMDAEMIAGYFRGKSVLVTGATGFLGKILVEKILRVQPEVKKIYLLVRAVDAQSAHQRVQDEVTDTEIFCLLKERHGKGGFELFVEEKIVALAGDVIHENLGLDAPRLEELAKEVDIIVNGAATTNFYERYDVSLDVNVMGVKHLCEFAHKCTNLKMFLHVSTAFAAGDREGLIMERPFKRGETLREGTRLDIDAELRLVADVKADLAAAGAGDGDDEKAERKAMKELGLRRSRHFGWSNTYVFTKAMGEMLLSQLRAGPAAAVAIMRPSIITSLLRDPLPGWMQGTRTIDTIIIGYAKQNLTCFLADLEGTMDVIPGDMVVNAMMAVMAAHGGQGSPEPGEEEVALYHATSSLRNPALYGVLYESGRRHFYENPRLSKDGKVIPTKEMYFFKTIASFHLYMLIKYKLPLEILHLVNLLLCGIFSQLYEDLSRKYKFVIHLCDVYGPFAFFRGCFDDMNLERLRLTMARTSPEDDLFNFDPKTVDWNHYFYKIHIPGVLKYVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1IC41</accession>
    <name>I1IC41_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U6 snRNA phosphodiesterase</fullName>
            <ecNumber evidence="5">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100839402</name>
        <name evidence="8" type="ORF">BRADI_3g50320v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 2H phosphoesterase superfamily. USB1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="5"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235776.1">
        <property type="nucleotide sequence ID" value="XM_010237474.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235777.1">
        <property type="nucleotide sequence ID" value="XM_010237475.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235778.1">
        <property type="nucleotide sequence ID" value="XM_010237476.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235779.1">
        <property type="nucleotide sequence ID" value="XM_010237477.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756901.1">
        <property type="nucleotide sequence ID" value="XM_014901415.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317562.1">
        <property type="nucleotide sequence ID" value="XM_024461794.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317563.1">
        <property type="nucleotide sequence ID" value="XM_024461795.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317564.1">
        <property type="nucleotide sequence ID" value="XM_024461796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IC41"/>
    <dbReference type="STRING" id="15368.I1IC41"/>
    <dbReference type="EnsemblPlants" id="PNT69148">
        <property type="protein sequence ID" value="PNT69148"/>
        <property type="gene ID" value="BRADI_3g50320v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69152">
        <property type="protein sequence ID" value="PNT69152"/>
        <property type="gene ID" value="BRADI_3g50320v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69153">
        <property type="protein sequence ID" value="PNT69153"/>
        <property type="gene ID" value="BRADI_3g50320v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69155">
        <property type="protein sequence ID" value="PNT69155"/>
        <property type="gene ID" value="BRADI_3g50320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839402"/>
    <dbReference type="Gramene" id="PNT69148">
        <property type="protein sequence ID" value="PNT69148"/>
        <property type="gene ID" value="BRADI_3g50320v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69152">
        <property type="protein sequence ID" value="PNT69152"/>
        <property type="gene ID" value="BRADI_3g50320v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69153">
        <property type="protein sequence ID" value="PNT69153"/>
        <property type="gene ID" value="BRADI_3g50320v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69155">
        <property type="protein sequence ID" value="PNT69155"/>
        <property type="gene ID" value="BRADI_3g50320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839402"/>
    <dbReference type="eggNOG" id="KOG3102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="KTVVLQY"/>
    <dbReference type="OrthoDB" id="49151at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IC41">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990838">
        <property type="term" value="F:poly(U)-specific exoribonuclease activity, producing 3' uridine cyclic phosphate ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034477">
        <property type="term" value="P:U6 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1140.10:FF:000008">
        <property type="entry name" value="U6 snRNA phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03040">
        <property type="entry name" value="USB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027521">
        <property type="entry name" value="Usb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522:SF3">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09749">
        <property type="entry name" value="HVSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT69148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="276" mass="31457" checksum="6417351582168569" modified="2012-06-13" version="1">MDALMANYASGSDDDEPTASTLENQEASALLPPPPLDLLQPPNFVDYSKMAQGSRIRSFPHVEGNYALHVYIPVVIPFDARKQLTIVMRRAASLVPDLYAVDADYALAELCKDEQKLEKVLLAREFHVSLGRTVAIQVHQIDSIVAMLRQKFQSQQSRYWMDFTKWEHFVNDDSTRSFLSLEVTRTGLSEITKQVHMVDEVYRLHGLPEFYKNPRPHISLVWALGDISSKLKQATKEIENFQNSVNSYKNCNLRCKFSRVVCKVGNKVYDICQAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1IC64</accession>
    <name>I1IC64_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF632 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100832160</name>
        <name evidence="4" type="ORF">BRADI_3g50480v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014755826.1">
        <property type="nucleotide sequence ID" value="XM_014900340.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IC64"/>
    <dbReference type="FunCoup" id="I1IC64">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IC64"/>
    <dbReference type="EnsemblPlants" id="KQK00585">
        <property type="protein sequence ID" value="KQK00585"/>
        <property type="gene ID" value="BRADI_3g50480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832160"/>
    <dbReference type="Gramene" id="KQK00585">
        <property type="protein sequence ID" value="KQK00585"/>
        <property type="gene ID" value="BRADI_3g50480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832160"/>
    <dbReference type="eggNOG" id="ENOG502QU01">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010985_7_0_1"/>
    <dbReference type="OMA" id="CRHGTHV"/>
    <dbReference type="OrthoDB" id="653690at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006868">
        <property type="entry name" value="DUF630"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006867">
        <property type="entry name" value="DUF632"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21450:SF11">
        <property type="entry name" value="OS02G0654600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21450">
        <property type="entry name" value="PROTEIN ALTERED PHOSPHATE STARVATION RESPONSE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04783">
        <property type="entry name" value="DUF630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04782">
        <property type="entry name" value="DUF632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF630" evidence="3">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="DUF632" evidence="2">
        <location>
            <begin position="146"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK00585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="571" mass="63224" checksum="868DEB48FE394162" modified="2012-06-13" version="1">MGTASSRHGDDGTAGRAAAREHTKRCRERRGLMREAARQRRQLAASHAGYLRSLSLVASTLTRFAVGEPLPVSDHTPPAVLVHRPVPVPSTPPPLLRSIEEQQPEEEAQEGVVGEAPFGGATTRMEGAGAGGGVQELRMEVRHRSLAEVAAGLEEYFVKVSVAGDAVSSVLETSNAEFRGGSNSFLGVLCCLSAPSVAHDRIDSMHGGRRHSSTLQQLVAWEKKLYKEVKARERVQIRHDKKLTELRDQEYSRKINVDIQKLKAAWDRSRAQLTTASQAVDATASAISDLRDAHLARQVLGLCHATRDMWKAMRQHHEAQCLIAQQLRGLSSRTSMDATTEIHHEATRALEAAMSAWHAALSHVVKHQRDYVHALHGWLQRTLTPVSSGGAEAAASRVLAVELADFVDRWKQVLDRVHCAEVLKSIKGFAGAVRALYAHQSDELRVARRVRQYSRELDRKSRMLRQVERSYYDSYVPSGISLWRPPMWGDDPRQVHSARNEVAQRKEEVAACRRMLEDEMRRHAKAIDATRSAAVTGVQGRLPAVFQAMAAFSASLANGLEAVCRQDNRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I1IC81</accession>
    <name>I1IC81_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100821609</name>
        <name evidence="9" type="ORF">BRADI_3g50610v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
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        <property type="protein sequence ID" value="KQK00603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003569973.3"/>
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    <dbReference type="EnsemblPlants" id="KQK00603">
        <property type="protein sequence ID" value="KQK00603"/>
        <property type="gene ID" value="BRADI_3g50610v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK00603"/>
        <property type="gene ID" value="BRADI_3g50610v3"/>
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    </dbReference>
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        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="14"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="138"/>
            <end position="222"/>
        </location>
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            <end position="404"/>
        </location>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK00603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="423" mass="46886" checksum="8B2B4A03F3FF709C" modified="2012-06-13" version="1">MFGRMPKKTSNNTKYYEVLGVSKTATPDELKKAYRKAAIKNHPDKGGDPEKFKELAHAYDVLNDPEKREIYDQYGEDALKEGMGGGSSDMHSPFDIFEQLFGGGGGGFGGGSSRGRRQKRGEDVVHTMKVSLEDLYNGATKKLSLSRNVLCGKCKGKGSKSGATATCHGCRGAGVRMITRQIGPGMIQQMNTVCPECRGAGEMISEKDKCPSCRGNKVAQEKKVLEVHVEKGMQHGQKIVFQGEADEAPDTVTGDIVFVLQLKEHPKFKRKSDDLYVEHTISLTEALCGFQFVLTHLDGRQLLIKSNPGEVVKPGQHKAINDEGMPQHGRPFMKGRLFVEFGVEFPEPGVLSPGQCRSLEKILPPRAGNQLSDMELDQCEETTMHDVNIEEEMRRRQHQRRQEAYDEEEEDDGAPRGVQCAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDE4</accession>
    <name>I1IDE4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein HEADING DATE REPRESSOR 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100826239</name>
        <name evidence="3" type="ORF">BRADI_3g53940v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK01118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003570243.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDE4"/>
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    <dbReference type="EnsemblPlants" id="KQK01118">
        <property type="protein sequence ID" value="KQK01118"/>
        <property type="gene ID" value="BRADI_3g53940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826239"/>
    <dbReference type="Gramene" id="KQK01118">
        <property type="protein sequence ID" value="KQK01118"/>
        <property type="gene ID" value="BRADI_3g53940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826239"/>
    <dbReference type="eggNOG" id="ENOG502RXJT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073521_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HDR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37205">
        <property type="entry name" value="F23A5.30 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37205:SF1">
        <property type="entry name" value="F23A5.30 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="186"/>
        </location>
    </feature>
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            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK01118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24136" checksum="B71B24AC111F8E93" modified="2012-06-13" version="1">MEEPSPADPPRIFWKARRRSAPANGRSLQVQELNDETAATREATNEDSMKIDDANAASTTADDGANHPDPKANLSEKRKALFEPLEPINGKRNSASMLLPPPDFEPASYPKGWLVGKKRKLVNVDVVESMRRIAIQEMNRKDREIGGLNEQLEEDSRVLELLQKQLADERRKRSEIEKENSMLQGQVSMLMNMLDENEAFDEEGEEVPPPDSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>I1IIA6</accession>
    <name>I1IIA6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100825853</name>
        <name evidence="9" type="ORF">BRADI_4g07060v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576163.1">
        <property type="nucleotide sequence ID" value="XM_003576115.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IIA6"/>
    <dbReference type="STRING" id="15368.I1IIA6"/>
    <dbReference type="EnsemblPlants" id="KQJ86675">
        <property type="protein sequence ID" value="KQJ86675"/>
        <property type="gene ID" value="BRADI_4g07060v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825853"/>
    <dbReference type="Gramene" id="KQJ86675">
        <property type="protein sequence ID" value="KQJ86675"/>
        <property type="gene ID" value="BRADI_4g07060v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825853"/>
    <dbReference type="eggNOG" id="KOG0176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_2_1"/>
    <dbReference type="OMA" id="RSMIDHA"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IIA6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140640">
        <property type="term" value="F:catalytic activity, acting on a nucleic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03753">
        <property type="entry name" value="proteasome_alpha_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000029">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033812">
        <property type="entry name" value="Proteasome_alpha_type_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 6 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ86675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="237" mass="25947" checksum="6A5BA47D98D642C1" modified="2012-06-13" version="1">MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKDGVVLAVEKRVTSPLLEPSSVEKIMEIDEHVGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQAICDLALRFGEGEEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKELTLQEAETIALSILKQVMEEKVTPNNVDIAKVAPNYHLYTPAEVEAVIGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJ10</accession>
    <name>I1IJ10_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aldehyde oxygenase (deformylating)</fullName>
            <ecNumber evidence="4">4.1.99.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100835573</name>
        <name evidence="14" type="ORF">BRADI_4g09090v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a long-chain fatty aldehyde + 2 NADPH + O2 + H(+) = a long-chain alkane + formate + 2 NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:21440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17176"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:83563"/>
            <dbReference type="EC" id="4.1.99.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sterol desaturase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.5" evidence="4"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577193.1">
        <property type="nucleotide sequence ID" value="XM_003577145.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJ10"/>
    <dbReference type="STRING" id="15368.I1IJ10"/>
    <dbReference type="EnsemblPlants" id="KQJ87030">
        <property type="protein sequence ID" value="KQJ87030"/>
        <property type="gene ID" value="BRADI_4g09090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835573"/>
    <dbReference type="Gramene" id="KQJ87030">
        <property type="protein sequence ID" value="KQJ87030"/>
        <property type="gene ID" value="BRADI_4g09090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835573"/>
    <dbReference type="eggNOG" id="KOG0873">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047036_5_3_1"/>
    <dbReference type="OMA" id="IVHEFIY"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071771">
        <property type="term" value="F:aldehyde oxygenase (deformylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000254">
        <property type="term" value="F:C-4 methylsterol oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080065">
        <property type="term" value="P:4-alpha-methyl-delta7-sterol oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="13">
        <location>
            <begin position="115"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ87030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="267" mass="30831" checksum="0FE0DB94273670CC" modified="2012-06-13" version="1">MAAASPLDSAWECLIDNFSEFQLATVVTFLLHESVFFLSGIPSLLFERFGLFAKYKIQKKSNAPAYQNRCVLRLILYHVGVNLPVMIFSYPAFKFMGLRSSLPLPHWTVIVSQILFYFVLEDFIFYWGHRALHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHSGYHFPWSPSNFLPLYGGSDFHDYHHRVLFTKSGNYASTFVYMDWLFGTDKGYRKIKAIEGEEGKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJW5</accession>
    <name>I1IJW5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plastocyanin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g11880v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJW5"/>
    <dbReference type="STRING" id="15368.I1IJW5"/>
    <dbReference type="EnsemblPlants" id="KQJ87553">
        <property type="protein sequence ID" value="KQJ87553"/>
        <property type="gene ID" value="BRADI_4g11880v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87553">
        <property type="protein sequence ID" value="KQJ87553"/>
        <property type="gene ID" value="BRADI_4g11880v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1899092_0_0_1"/>
    <dbReference type="InParanoid" id="I1IJW5"/>
    <dbReference type="OrthoDB" id="694051at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ87553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="14815" checksum="C31C76E477BB4955" modified="2012-06-13" version="1">MAPWLSGRDPPAAAAAFGSYPFPKAHEEIPIIIGEWWEMDLVELDRRMVNGFFHDNPSAAMINGKPGDLYNCSGNLEPIDFTTIAAELVRQDAIHVSQNMKIDAAEHVAALDNDVRETKLFHIGMKVDSTSSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJZ8</accession>
    <name>I1IJZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GTPase Der</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GTP-binding protein EngA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100845933</name>
        <name evidence="10" type="ORF">BRADI_4g12180v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237502.1">
        <property type="nucleotide sequence ID" value="XM_010239200.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJZ8"/>
    <dbReference type="STRING" id="15368.I1IJZ8"/>
    <dbReference type="EnsemblPlants" id="KQJ87599">
        <property type="protein sequence ID" value="KQJ87599"/>
        <property type="gene ID" value="BRADI_4g12180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845933"/>
    <dbReference type="Gramene" id="KQJ87599">
        <property type="protein sequence ID" value="KQJ87599"/>
        <property type="gene ID" value="BRADI_4g12180v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845933"/>
    <dbReference type="eggNOG" id="KOG1191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="CNLPQYV"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IJZ8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042793">
        <property type="term" value="P:plastid transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01894">
        <property type="entry name" value="EngA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01895">
        <property type="entry name" value="EngA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000004">
        <property type="entry name" value="GTPase Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000040">
        <property type="entry name" value="GTPase Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001185">
        <property type="entry name" value="GTPase Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00195">
        <property type="entry name" value="GTPase_Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031166">
        <property type="entry name" value="G_ENGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016484">
        <property type="entry name" value="GTPase_Der"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032859">
        <property type="entry name" value="KH_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03594">
        <property type="entry name" value="GTPase_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43834">
        <property type="entry name" value="GTPASE DER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43834:SF2">
        <property type="entry name" value="GTPASE DER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14714">
        <property type="entry name" value="KH_dom-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51712">
        <property type="entry name" value="G_ENGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="EngA-type G" evidence="9">
        <location>
            <begin position="145"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G" evidence="9">
        <location>
            <begin position="359"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="623"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="623"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ87599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="648" mass="71355" checksum="3FD2D74BC06AC018" modified="2012-06-13" version="1">MAMASTSSCSSRPPLLHPASRTPNPSRLPPLVSRASSSPRPLLLSFAPPARGRFRAAAVQQSDFEDGEEEWDGEVEEEYYREEEEVEEGEEMDVEAMEEEARAAAADLAARLDRELRVDGDVREKRRTMRDKTSTSKHIPDNNLPKVAIIGRPNVGKSALFNRLVGSNRAIVVDEPGVTRDRLYGRSYWGDQEFMVIDTGGVITLSKSQAGVMEELAITTTVGMDGIPLASREAAIARMPSMIEKQAVAAVEEATVLLFLVDGQAGLVAADIEIADWLRRNFSHKCIILAVNKCESPKKGQMQALEFWSLGFTPVSISAITGTGTGELLDMVCSELKNFEVLQGLEGLDDVEEDENRVPAISIVGRPNVGKSSILNALVGEDRTIVSPVSGTTRDAIDTEFTTEDGQKFKLIDTAGIRRRAAVASAGSTTETLSVKRAFSAIRRSDVVALVVEAMACVTEQDYKIAERIEKEGKACVIVVNKWDTIPNKNNESTTHYELDVREKLRVLDWAPIVYCSAINGNSVEKIISAASLVEKERSRRLGTSILNQVVREAVAFKAPPRTRGGKRGRVYYTTQAAVRPPTFVLFVNDAKLFPEPYRRYMHKQLRSDAGFPGTPIRLLWRSRKRADKQRRKSNNTEARGSLVGASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IP98</accession>
    <name>I1IP98_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mediator of RNA polymerase II transcription subunit 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100843505</name>
        <name evidence="5" type="ORF">BRADI_4g27747v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mediator complex subunit 25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238042.1">
        <property type="nucleotide sequence ID" value="XM_010239740.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IP98"/>
    <dbReference type="FunCoup" id="I1IP98">
        <property type="interactions" value="1197"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IP98"/>
    <dbReference type="EnsemblPlants" id="KQJ89787">
        <property type="protein sequence ID" value="KQJ89787"/>
        <property type="gene ID" value="BRADI_4g27747v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843505"/>
    <dbReference type="Gramene" id="KQJ89787">
        <property type="protein sequence ID" value="KQJ89787"/>
        <property type="gene ID" value="BRADI_4g27747v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843505"/>
    <dbReference type="eggNOG" id="ENOG502QR8W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010796_0_0_1"/>
    <dbReference type="OMA" id="NQMHQQT"/>
    <dbReference type="OrthoDB" id="7690434at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021419">
        <property type="entry name" value="Mediator_Med25_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12433">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12433:SF11">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11265">
        <property type="entry name" value="Med25_VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 25 von Willebrand factor type A" evidence="4">
        <location>
            <begin position="8"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="770"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ89787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="820" mass="88574" checksum="428C22B53B21CF74" modified="2012-06-13" version="1">MVEGMAAERQLVVVVEGTAALGPYWHTIAAEYVEKIVRSFCAAQLSGQKLAGVPPELALVVFHTHGPYSAFVVQRSGWTKDMDTFLSWLSGISFSGGGFSEAAICEGLAEALMILQGSPSNNQNHQNHELQKHCVLVAASNPYPLPTPVYRPFVQSGDHKKNNEVTKESCLADAEAVAISFSQCCVSLSVVSPKQLPTLKAIYNAGKRNPQASDPSVDHVKNPHFLVLLSESFMEARTALSHPSPGNLVPNQIITKMDIAPAATVPGPTSNANPSVNGSMMGRQPTANIKVEPTIITPMVSAPAFSHMTPISNVTSQGVSALQSSSPSIISQETNVANEILQEHKPLVNPIQQQVRPGGPANVSILNNLSQHRHSLSGATSMGPNMGATPIQVHMSNMISSGMTSTPSVISSMSGTVQPTGAQQLVQNTALGSFGSNNSTVSGNSNVAVSSSLANIQNNIATAQSVPPMAQGGLMSGSHGSQSGQGGIGTNQNMISSLGPTAISSAPAMMPTPGMTQQAGVNSLSVNNSSAMNMPMPQQPNGQQQSKYVKIWEGTLSGQRQGQPVFICKLEGYRSGTASDTLAADWPETMQIVRLIAQEHMNNKQYVGKADFLVFRTLNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKAGRLIGMLFPGDMVVFKPQVSTQQPQMQQQQQLQQQHHPLHQQQLQQLQQQQLQQQHMQMQPQGQQLQQQQQIQQQMQQQQQQQQQMQQMQHQQQQQQQIQQQQQMQQQQQQMQQMQQQQPQQLQQQPQMVGPGMGQQQFMQGHGRAVQMMQGKIAPQGPGSMSGGGYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPT4</accession>
    <name>I1IPT4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100833306</name>
        <name evidence="2" type="ORF">BRADI_4g29285v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576478.2">
        <property type="nucleotide sequence ID" value="XM_003576430.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPT4"/>
    <dbReference type="EnsemblPlants" id="PNT64493">
        <property type="protein sequence ID" value="PNT64493"/>
        <property type="gene ID" value="BRADI_4g29285v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833306"/>
    <dbReference type="Gramene" id="PNT64493">
        <property type="protein sequence ID" value="PNT64493"/>
        <property type="gene ID" value="BRADI_4g29285v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833306"/>
    <dbReference type="eggNOG" id="ENOG502R3VA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1743052_0_0_1"/>
    <dbReference type="OMA" id="AHANGQF"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT64493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="150" mass="15281" checksum="5E75E3F4F8D1F0F9" modified="2012-06-13" version="1">MQSNLTRSPSTTATTTTRKAPAISNEKQRGSSGMERFAAMVTGRRAAPAPKPASAAAESEGDKEYLRIQLEEIVIVKDDAYDALAAATAAARAHAHPHPHAHANGQFSSAAASTSMENCAKAAVAAPHGGPPMAAAPPGAWASVGRIVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITQ4</accession>
    <name>I1ITQ4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I and III subunit RPAC2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100839460</name>
        <name evidence="8" type="ORF">BRADI_4g40550v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578801.1">
        <property type="nucleotide sequence ID" value="XM_003578753.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITQ4"/>
    <dbReference type="STRING" id="15368.I1ITQ4"/>
    <dbReference type="EnsemblPlants" id="KQJ91915">
        <property type="protein sequence ID" value="KQJ91915"/>
        <property type="gene ID" value="BRADI_4g40550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839460"/>
    <dbReference type="Gramene" id="KQJ91915">
        <property type="protein sequence ID" value="KQJ91915"/>
        <property type="gene ID" value="BRADI_4g40550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839460"/>
    <dbReference type="eggNOG" id="KOG3438">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090381_4_0_1"/>
    <dbReference type="OMA" id="ITHATFC"/>
    <dbReference type="OrthoDB" id="510325at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07029">
        <property type="entry name" value="RNAP_I_III_AC19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerases I and III subunit RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033898">
        <property type="entry name" value="RNAP_AC19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF28">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="7">
        <location>
            <begin position="12"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ91915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="108" mass="11943" checksum="83197B9409F7B5B7" modified="2012-06-13" version="1">MEHGSVADSTASSFSIMEEDHTLANSVRFVLNQDPRVAFCGYSIPHPADNKVNIRVQTTGDPAKDVMVDALQDLMVMCQHARGTFDTAVADFRANKPMERMDIDLNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IW79</accession>
    <name>I1IW79_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100835382</name>
        <name evidence="8" type="ORF">BRADI_5g03300v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the detoxification of the Fusarium mycotoxin deoxynivalenol by the transfer of glucose from UDP-D-glucose to the hydroxyl group at C-3, forming deoxynivalenol-3-O-beta-D-glucoside.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239695.1">
        <property type="nucleotide sequence ID" value="XM_010241393.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IW79"/>
    <dbReference type="SMR" id="I1IW79"/>
    <dbReference type="FunCoup" id="I1IW79">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ81826">
        <property type="protein sequence ID" value="KQJ81826"/>
        <property type="gene ID" value="BRADI_5g03300v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835382"/>
    <dbReference type="Gramene" id="KQJ81826">
        <property type="protein sequence ID" value="KQJ81826"/>
        <property type="gene ID" value="BRADI_5g03300v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835382"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001724_0_1_1"/>
    <dbReference type="OMA" id="DYMESTW"/>
    <dbReference type="OrthoDB" id="678493at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IW79">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098754">
        <property type="term" value="P:detoxification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000057">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF732">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0216">Detoxification</keyword>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00058521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ81826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="469" mass="51703" checksum="75E25231788B5286" modified="2012-06-13" version="1">MDSTGKSVMATSEGPSILFLPFPGAQGHANPMLQFGHRLAYQYGFRPTLVVTRYVLSTALPPDAPFRVAAISDGFDAGGIRSCLDMAEYWRRLEAVGSETLSRLISDEAREGRPVRVLVYDPHVAWARRVAREAGVPAAAFFSQPCAVDIFYGELHAGRMAMPVTEADARALLVRGAIGVELALDDVPPFVVVPESQPVFTKASIGQFEGLEDADDVLVNSFRDIEPMEVEYMESTWRAKTIGPTLPSFYLDDDRLPSNKSYGFNLFNGGDAVCMKWLDQQSMSSVVLVSYGTVSNYDESQLEELGNGLCSSGKPFIWVVRSNEAHKLSGELKAKCEKKGLIVSWCPQLEVLAHKATGCFLTHCGWNSTLEAIVNGVPVVGIPHWADQPTIAKYVESAWDMGVRVKKSLNGQLRREEIERCIKEVMDSERKDEYTRNAAKWMQKAKETMHAGGSSNKHIAEFAAKYSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZT9</accession>
    <name>I1IZT9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100826404</name>
        <name evidence="9" type="ORF">BRADI_5g16170v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580167.1">
        <property type="nucleotide sequence ID" value="XM_003580119.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZT9"/>
    <dbReference type="FunCoup" id="I1IZT9">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IZT9"/>
    <dbReference type="EnsemblPlants" id="KQJ83669">
        <property type="protein sequence ID" value="KQJ83669"/>
        <property type="gene ID" value="BRADI_5g16170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826404"/>
    <dbReference type="Gramene" id="KQJ83669">
        <property type="protein sequence ID" value="KQJ83669"/>
        <property type="gene ID" value="BRADI_5g16170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826404"/>
    <dbReference type="eggNOG" id="ENOG502QQ2H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015477_2_0_1"/>
    <dbReference type="OMA" id="ACQWKVF"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF76">
        <property type="entry name" value="METAL-NICOTIANAMINE TRANSPORTER YSL8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="499"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="537"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="598"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="635"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ83669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="717" mass="78182" checksum="4B06A7DB7333208A" modified="2012-06-13" version="1">MAPRTTEEDGSAGDEESPVEASTLRHRHNTGKNGAGEDDEGKRNGAALEEEEEEASVERAFEGKAVPTWREQLTLRAFVVSFFLAIMFSVIVMKLNLTTGIIPSLNVSAGLLGFFFVRLWTGAIERMGFLRQPFTRQENTVIQTCVVAAYDIAFSGGFGNYLFAMSDTIANQTTEAKDAQNIKNPHVGWMIAFLFLVSFVGLFALVPLRKIMIVDYKLTYPSGTATAYLINGFHTPEGAKLAKKQVKTLGKFFLFSFLWGFFQWFYTGGDSCGFSSFPTLGLQAYKNRFYFDFSPTYVGVGMICPHIVNVSVLLGGILSWGVMWPLISNKRGIWFSADLPDSSLHGLQGYRVFISISLILGDGLYNFLKVLVRTTAAFATMVKKNNMIPVSDEGGAMVTGEAVSYDDQRRTELFLRDQIPKTVAYAGYAAVAAVSIATLPLLFPQLKWYYILVAYLFAPVLAFCNAYGTGLTDWSLASTYGKLAIFIFGAWAGAPHGGVLVGLAACGVMMSIVSTAADLMQDFKTGYLTLASPRSMFISQVIGTAMGCVIAPCVFWLFYKAFTDIGISGSEYPAPYAIVYRNMAILGVDGFSSLPKNCLTLCFIFFAAAIVINLARDLAPSKVAKFIPLPMAMAIPFYIGSYFAIDMFVGSVILFVWGIVNKAKADAFAPAVASGLICGDGIWTLPQSILALAKVKPPICMKFLSRSVNAKVDDFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1J033</accession>
    <name>I1J033_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peroxisomal membrane protein 11-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100844334</name>
        <name evidence="8" type="ORF">BRADI_5g16860v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peroxisomal proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxin-11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580226.1">
        <property type="nucleotide sequence ID" value="XM_003580178.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J033"/>
    <dbReference type="STRING" id="15368.I1J033"/>
    <dbReference type="EnsemblPlants" id="KQJ83788">
        <property type="protein sequence ID" value="KQJ83788"/>
        <property type="gene ID" value="BRADI_5g16860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844334"/>
    <dbReference type="Gramene" id="KQJ83788">
        <property type="protein sequence ID" value="KQJ83788"/>
        <property type="gene ID" value="BRADI_5g16860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844334"/>
    <dbReference type="eggNOG" id="KOG4186">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080291_0_0_1"/>
    <dbReference type="OMA" id="LVHWHVE"/>
    <dbReference type="OrthoDB" id="411017at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044375">
        <property type="term" value="P:regulation of peroxisome size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF17">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 11B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ83788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25054" checksum="187552E417DF1EF3" modified="2012-06-13" version="1">MSAGDTLDKLVVFLAKRDGIDKLVKTYQYVSKLAHWAAESSHPDLARRAKNWETAAGLSRKVFRSGRSLTGFNTLRRSPGEFGALAVLANAGEMVYFFFDHFTWLSRVGVLEPWLARRMSFVSAFGESVGYVFFIAMDFIMLRRGIRQERKLLREGGGGKEKEGELSKIRTDRVMRLMGTAANLADLVIAIADIEPNPFCNHAVTLGISGLVSAWAGWYRNWPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0B2</accession>
    <name>I1J0B2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100833343</name>
        <name evidence="3" type="ORF">BRADI_5g17550v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580275.1">
        <property type="nucleotide sequence ID" value="XM_003580227.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0B2"/>
    <dbReference type="EnsemblPlants" id="KQJ83919">
        <property type="protein sequence ID" value="KQJ83919"/>
        <property type="gene ID" value="BRADI_5g17550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833343"/>
    <dbReference type="Gramene" id="KQJ83919">
        <property type="protein sequence ID" value="KQJ83919"/>
        <property type="gene ID" value="BRADI_5g17550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833343"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_21_6_1"/>
    <dbReference type="OMA" id="MERSHKI"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052451">
        <property type="entry name" value="Ser/Thr_kinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48008">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48008:SF13">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="82"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ83919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="363" mass="39799" checksum="D59B789A723FB228" modified="2012-06-13" version="1">MRGTIVNAILFPVLAVIVLLSLCYCIRCYRRRRRRHGVLPSHGARADRFQAAGSSAYGTGAGEELLVRFPGGEGLTVAAILEAPGEVVAKSAHSTLYRAGLSAGEAVALLRFLRPVCSAAAEEAAAAARILGAVQHPNLVPIRALYVGPRGEKLLVHPFYAAGSLRRFLQEGINDSQRWEIICKLSMGIVKGLDHLHTRSQRPIIHGNLKANNIMLDADFQPRISDFGLYLLLNPSASQDMLETSAVQGYKAPELIKMKEVTRESDIYSLGVILLEMLAQKEAANDSQPNPRDIHLPASFKDLVLERKISDAFSSELIKQSKNSGKERNLNAFFELATACCNPSPSLRPDTNRILKRLEEIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>I1J2V1</accession>
    <name>I1J2V1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17 18">Plasma membrane ATPase</fullName>
            <ecNumber evidence="3 18">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100846063</name>
        <name evidence="20" type="ORF">BRADI_5g24690v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Cell membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="3 18"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_003580730.1">
        <property type="nucleotide sequence ID" value="XM_003580682.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J2V1"/>
    <dbReference type="SMR" id="I1J2V1"/>
    <dbReference type="FunCoup" id="I1J2V1">
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    </dbReference>
    <dbReference type="STRING" id="15368.I1J2V1"/>
    <dbReference type="EnsemblPlants" id="KQJ85071">
        <property type="protein sequence ID" value="KQJ85071"/>
        <property type="gene ID" value="BRADI_5g24690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846063"/>
    <dbReference type="Gramene" id="KQJ85071">
        <property type="protein sequence ID" value="KQJ85071"/>
        <property type="gene ID" value="BRADI_5g24690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846063"/>
    <dbReference type="eggNOG" id="KOG0205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_6_4_1"/>
    <dbReference type="OMA" id="GHFELVM"/>
    <dbReference type="OrthoDB" id="116380at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
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        <property type="term" value="P:proton export across plasma membrane"/>
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        <property type="term" value="P:proton transmembrane transport"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 18" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="14 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="239"/>
            <end position="265"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="645"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="710"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="751"/>
            <end position="772"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="784"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="817"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="13"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012476"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="RuleBase" id="RU362083"/>
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    </evidence>
    <evidence type="ECO:0000259" key="19">
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            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KQJ85071.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85071"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="951" mass="104783" checksum="67AD6F3B84328EB6" modified="2012-06-13" version="1">MGGLEEIRNEAVDLENIPIEEVFEQLKCTREGLTSDEGAQRVTIFGLNKLEEKKESKVLKFLGFMWNPLSWVMEMAAIMAIALANGEGKPPDWQDFVGIIVLLVINSTISFIEENNAGNAAAALMANLAPKTKVLRDGRWGEQEASILVPGDIVSIKLGDIVPADARLLEGDPLKIDQSGLTGESLPVTKNPGDEVFSGSTCKQGEIEAVVIATGVHTFFGKAAHLVDSTNQVGHFQKVLTAIGNFCIVSIAVGIVIEIIVMFPIQRRKYRAGIENLLVLLIGGIPIAMPTVLSVTMAIGSHKLSQQGAITKRMTAIEELAGMDVLCSDKTGTLTLNKLSVDKNLVEVFAKGVDKEHVLLLAARASRVENQDAIDACMVGMLADPKEARAGIREVHFLPFNPTDKRTALTYIDAEGNWHRASKGAPEQIITLCNCKEDVKRKVHSVIEKYAERGLRSLAVARQEVPEKSKDSPGGPWQFIGLLPLFDPPRHDSAETIRKALVLGVNVKMITGDQLAIGKETGRRLGMGTNMYPSSALLGQSKDGSLESLPVDELIEKADGFAGVFPEHKYEIVKRLQEKKHIVGMTGDGVNDAPALKKADIGIAVDDATDAARSASDIVLTEPGLSVIISAVLTSRCIFQRMKNYTIYAVSITIRIVLGFMLIALIWKFDFAPFMVLIIAILNDGTIMTISKDRVKPSPLPDSWKLNEIFATGVVLGTYLALMTVVFFWAIHKTDFFTNKFGVRSIRNSEFELMSALYLQVSIVSQALIFVTRSRSWSFVERPGFLLVTAFLLAQLVATLIAVYANWEFARIKGIGWGWAGVIWLFSIVFYFPLDVFKFFIRFVLSGRAWDNLLQNKTAFTTKKDYGRGEREAQWATAQRTLHGLQAPESNNNTLFNDKSSYRELSEIAEQAKRRAEIARLRELNTLKGHVESVVKLKGLDIDTINQNYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E2Q2</accession>
    <name>A0A0Q3E2Q2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_5g05173v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E2Q2"/>
    <dbReference type="EnsemblPlants" id="KQJ82047">
        <property type="protein sequence ID" value="KQJ82047"/>
        <property type="gene ID" value="BRADI_5g05173v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82047">
        <property type="protein sequence ID" value="KQJ82047"/>
        <property type="gene ID" value="BRADI_5g05173v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E2Q2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ82047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="72" mass="7938" checksum="6A9FB0D836913284" modified="2016-01-20" version="1">MVGGSCNATAHRGRRASEQSMQYRVGEQQASRPHASTSSNFLIPIVVCLLFLFSIINGFVTSRASYHCDFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EM71</accession>
    <name>A0A0Q3EM71_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100834947</name>
        <name evidence="2" type="ORF">BRADI_4g20030v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576077.2">
        <property type="nucleotide sequence ID" value="XM_003576029.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757677.1">
        <property type="nucleotide sequence ID" value="XM_014902191.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318564.1">
        <property type="nucleotide sequence ID" value="XM_024462796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EM71"/>
    <dbReference type="EnsemblPlants" id="KQJ88631">
        <property type="protein sequence ID" value="KQJ88631"/>
        <property type="gene ID" value="BRADI_4g20030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834947"/>
    <dbReference type="Gramene" id="KQJ88631">
        <property type="protein sequence ID" value="KQJ88631"/>
        <property type="gene ID" value="BRADI_4g20030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834947"/>
    <dbReference type="OrthoDB" id="585353at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EM71">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165:SF36">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="98"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ88631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="438" mass="48077" checksum="34C84C846AE5FE1C" modified="2016-01-20" version="1">MAMAEARAATGHRGRGPAPSWVLMQEDMLQLIASRVLAGDLLDYVRFRAVCLPWRAATACPRGQGLVNPLFHPRRWMMLPEGDGMHPGHPALGGYGRFFNLDTAAFVRVRLPCFQDQDHIVLDCPDGLLLLQRKGDGAICLHHPFTGDIAQFPPLASLITQLDGAVGVAGVDARLLLRFDRFMDVSAAVSVRAGGTVTIMLAFIRLQRMAYVSTGDLQWTATSWMIPGMWTAVPFRGSLYLVKGWNKRKPSLIMRVDPPECSSSVLWSSAPAQTVATCPAEKMTKPYLVECNSELLVVGYAKADRHSLVVFRLADLLLGAPTPAAPLASIGDHVLFIGKRNMTVKSKNIPVLRGNSVAIITASAADRLLQYNLGRGAWSQLCDGDLINGPAPRPHSLVHHIVTCCHHAFWSSGHIWYVKSTEPWRRMTAGAYIKQYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F190</accession>
    <name>A0A0Q3F190_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="10">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100827728</name>
        <name evidence="13" type="ORF">BRADI_4g42950v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(E)-4-coumarate + ATP + CoA = (E)-4-coumaroyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19641"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19642"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(E)-4-coumarate + ATP + H(+) = (E)-4-coumaroyl-AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:72419"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:72420"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(E)-4-coumaroyl-AMP + CoA = (E)-4-coumaroyl-CoA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:72423"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:72424"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="10"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578933.2">
        <property type="nucleotide sequence ID" value="XM_003578885.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F190"/>
    <dbReference type="FunCoup" id="A0A0Q3F190">
        <property type="interactions" value="2055"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3F190"/>
    <dbReference type="EnsemblPlants" id="KQJ92332">
        <property type="protein sequence ID" value="KQJ92332"/>
        <property type="gene ID" value="BRADI_4g42950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827728"/>
    <dbReference type="Gramene" id="KQJ92332">
        <property type="protein sequence ID" value="KQJ92332"/>
        <property type="gene ID" value="BRADI_4g42950v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827728"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3F190">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016207">
        <property type="term" value="F:4-coumarate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106290">
        <property type="term" value="F:trans-cinnamate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="12">
        <location>
            <begin position="136"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ92332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="722" mass="79489" checksum="3F93B2A647DD79A9" modified="2016-01-20" version="1">MWTRGTEEAMAREEEQQRQEQEERSQAMDAAQRRLRAVSAHLEPPTQVDTNGGGLAANPTAGEYAHVQGYSVVLPEKLQTGKWNVHRSAQSPLRLISRFPSTPDIGTLHDNFLYAVETFTDCRYLGTRLSANGAVGEYKWMTYGEASTSRTAIGSGLIYHGIAEGARIGLYFINRPEWIIIDHACAAYSYVSVPLYDTLGPDAVQFIVNHATVEAIFCVPQTLSSLLSFITQMPCVRLIVVVGGDDTNTPSATASTGVEIITYSRLHSQGKMSSQTFRPPKPEDVATICYTSGTTGTPKGAVLSHENLIANVAGSSLVVKFYPSDVYISYLPLAHIYERANQIALLHYGVAIGFYQGDNLKLMDDLAALRPTVFASVPRLYNRIYSAITNAVKESGGLKERLFRTAYNAKRQALMSGRNPSPMWDKLVFNKIKARLGGRVRLMTSGASPLSADVMEFLRICFGGEVLEGYGMTETSCVITTMDIGDRLIGHVGSPNPSCEIKLVDVPEMNYTSEDQPHPRGEICVRGPAIFRGYYKDEVQTRDVIDGDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIENVYAKCKFIAQCFIYGDSFNSFLVAIVAVEPDVLKAWAGSEGIQSEDLRQLCADPRAKAAVLADMDSIGKEAQLRGFEFAKAVSLVAEPFTVENGLLTPTFKIKRPQAKAYFAKEIADMYAQLSKAESARQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FHT3</accession>
    <name>A0A0Q3FHT3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerases I, II, and III subunit RPABC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g32320v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FHT3"/>
    <dbReference type="STRING" id="15368.A0A0Q3FHT3"/>
    <dbReference type="EnsemblPlants" id="KQJ97628">
        <property type="protein sequence ID" value="KQJ97628"/>
        <property type="gene ID" value="BRADI_3g32320v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97628">
        <property type="protein sequence ID" value="KQJ97628"/>
        <property type="gene ID" value="BRADI_3g32320v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FHT3"/>
    <dbReference type="OrthoDB" id="10258858at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FHT3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF9">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT 10-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ97628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11890" checksum="F096B67C45BD85BB" modified="2016-01-20" version="1" fragment="single">AKSFCNVVFLLTLPSPENFSSPLLSGSGGRRATSKMIIPVHYFTCGKVIGNKWDHYLDLLQADYTEGAALDTLGLVRYCCRRMLMTHVDLIEKLLNYNTLEKTESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FKM5</accession>
    <name>A0A0Q3FKM5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g45365v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FKM5"/>
    <dbReference type="FunCoup" id="A0A0Q3FKM5">
        <property type="interactions" value="2469"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99814">
        <property type="protein sequence ID" value="KQJ99814"/>
        <property type="gene ID" value="BRADI_3g45365v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99814">
        <property type="protein sequence ID" value="KQJ99814"/>
        <property type="gene ID" value="BRADI_3g45365v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FKM5"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009960">
        <property type="term" value="P:endosperm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044278">
        <property type="entry name" value="BHLH95-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46772">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46772:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="56"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ99814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="276" mass="29026" checksum="87EA66785995366F" modified="2016-01-20" version="1">MSSGSNLIRAGAVNKGKGIVKEEEQAEEGTGGSKGDKLMSAAQAAAGRAGGSRRRGRRAKHITTERERRKRMSEMFSTLHGLLPSLPDKMDKSSIVMEAIHHIKTLEGTVKELEKRKQDLARGMPAGIGSSVSGISSSSPAVVLPVPAAVVVQPPAAGAGAGAVPPQLGLQTWSGQNVALSLSGDDAYINVCAPRYGPRMLKLVVSVLDKHGLEVITSGIASESSRVMFNFHTRVNAANSVFGDRLSSEDLCKLAVSEIMIWLSSAKYCLHGTSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FW78</accession>
    <name>A0A0Q3FW78_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4220 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_2g01485v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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            <strain evidence="4 5">Bd21</strain>
        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_2g01485v3"/>
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            <strain evidence="2 3">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Bevan M.W."/>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <property type="gene ID" value="BRADI_1g30317v3"/>
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        </source>
    </evidence>
    <sequence length="730" mass="83208" checksum="B0FB7845468E2BC9" modified="2018-03-28" version="2" fragment="single">MAHNVGPLLGRRRISHLQYMISHLRHLIFFTGFSQPAISLPVAEQLWNQWEIQSLVLVSFSLQVFLLFFPAIRKRRHSSIAYLLADYVATFTLGRLTLHLDEPRHQLLLFWTPFLLLHLGGQETIAALSTEDSLLWKRHLLSLVSQVLLAIYIVTKSWHVVSNKHLMAPMVLMFISGTIKYAERTWALMAAGSAMTPGSSSTLSDYVLNVEGSVLDDAQSYFQRLHDYLSATDHKGRIDYQGLVEVAGKGVRICLDFLTDMTPFLMWHKGNIIDRSIKKFQDLKKQEHRHKIAYKLAEIQLSLIYDFMYTKYGVLQYHLNMVTSGIQRFVTFGTTTVALGLFLKADLEGHFSNDVRADVVVSYILLGGAVALDLSSIFLVLSSYWPYLPGRNPFGKDAGFPGKRLMFSLTTRLVYPMRKSFWSGQMSQYNLIGECIHEKEANLLVKVLLKVGLMSDVKTVQVSPELKEFVFRKLLDTGATRHVNEYWKWDSSAFTGQWARWALEKTQEGKSVDQSVLNLEGRRNHRCAMDKDSTTVPYRNMSLHMSDYIMYLVGKCGVKSGSNGHFELGKLRRDVRKAISDRSHFSRGRLEQSKVVMYGYEGRGFFTSRASAAAKELRKITDPSDRWEIIATVWAEMLCYLSQNCEAVFHIKSLSTGGEFITHVRILSIILGIPFLREAWLTTAANGKPIRGEDAILQEQPREDLALWLSMCLPNLEGRKAWVRANELRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HB77</accession>
    <name>A0A0Q3HB77_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100825065</name>
        <name evidence="2" type="ORF">BRADI_1g52980v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228180.1">
        <property type="nucleotide sequence ID" value="XM_010229878.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HB77"/>
    <dbReference type="FunCoup" id="A0A0Q3HB77">
        <property type="interactions" value="1354"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3HB77"/>
    <dbReference type="EnsemblPlants" id="KQK20175">
        <property type="protein sequence ID" value="KQK20175"/>
        <property type="gene ID" value="BRADI_1g52980v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825065"/>
    <dbReference type="Gramene" id="KQK20175">
        <property type="protein sequence ID" value="KQK20175"/>
        <property type="gene ID" value="BRADI_1g52980v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825065"/>
    <dbReference type="OrthoDB" id="1882251at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HB77">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33527">
        <property type="entry name" value="OS07G0274300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33527:SF14">
        <property type="entry name" value="OS07G0274300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK20175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="40969" checksum="F0E803C2015FD6C6" modified="2016-01-20" version="1">MEPDLFNAANSYYMILRGLFFRLVVSLQIESSLSMEIIAFWLWIQGNGYEDYLAHSDLFDDNYFLMFASTGKIFLEVLHIEFDDSDPRSIPSRSFLREAIEGISFYLNNICYKALEDLRERAEIHAMNQAFGESFFEQQAYGESFLERQAYGESFFEQQAYEEYLNNRVPISPEHLLTKIKALYTNTSKHHGEGTSSRSIRHPTSHVPQDRGEEVYERQSTSRLATLLENLSLREKHNDGIMQQLSDVPRDERTLFVTFSNGYPLTKDELYDFFMRHYGDIEEITIEEPMEQRPPLYAHVTFFSQLTLFRVLDGNKKVKFMTRQKHLWARQYVPKKKKTKPNELNPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HCG8</accession>
    <name>A0A0Q3HCG8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_4g36120v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HCG8"/>
    <dbReference type="STRING" id="15368.A0A0Q3HCG8"/>
    <dbReference type="EnsemblPlants" id="KQJ91178">
        <property type="protein sequence ID" value="KQJ91178"/>
        <property type="gene ID" value="BRADI_4g36120v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ91178">
        <property type="protein sequence ID" value="KQJ91178"/>
        <property type="gene ID" value="BRADI_4g36120v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HCG8"/>
    <dbReference type="OrthoDB" id="7130006at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HCG8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="32"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ91178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="272" mass="29397" checksum="6290DD9E4C12C037" modified="2016-01-20" version="1">MATGGAGEVRHWTASVNGITLHVAEQGPATGPAVLLLHGFPELWLSWRHQMSALAARGFRALAPDLRGYDDSEVPAGGAAEYTMLHVVGDVVALLDHLGLLDALVAGHDWGAQVLWHLCLFRPDRVRAAVALGVPYFPRSPAPMADFLAARGDGFYISQFQEPGRAEKAFAKHDIATVLKKFYSLELDDLSAPPGVEVIDFFQASSSPLPWMTEEELAGTPTSSERPASRGPSTTTAPWTCKFEASFITSLHCHLWSSSILFDSGSVLMLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HJI1</accession>
    <name>A0A0Q3HJI1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100840954</name>
        <name evidence="5" type="ORF">BRADI_4g18390v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757818.2">
        <property type="nucleotide sequence ID" value="XM_014902332.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HJI1"/>
    <dbReference type="STRING" id="15368.A0A0Q3HJI1"/>
    <dbReference type="EnsemblPlants" id="KQJ88483">
        <property type="protein sequence ID" value="KQJ88483"/>
        <property type="gene ID" value="BRADI_4g18390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840954"/>
    <dbReference type="Gramene" id="KQJ88483">
        <property type="protein sequence ID" value="KQJ88483"/>
        <property type="gene ID" value="BRADI_4g18390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840954"/>
    <dbReference type="OrthoDB" id="1936481at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GRAS"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="117"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="176"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="458"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="533"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="666"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="489"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="216"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ88483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="743" mass="80506" checksum="09874484D0A80825" modified="2016-01-20" version="1">MCSRMGTLNCSDTTSSVKLQQQQGPTSPTASFSESNIVASSTDPDAIDALAGLQALRFDGDIDGEIQSPDLAMWESLFADQIGASGADFLMSSPRRDFSPLRDFMVSSPKRDYMVSSPKRDYMMSSPKRDYMMSSPKRDYMVSSPKREMGVSSPRRSTFSNLYSSTINQANQQSYMHGMEGSPQTQYSNLASQGNKGKSSPSPLHKVYINNVNAHSNSGKSNGPSSLSCSSSYAHGENLPLPSMDPFLEEYKEGYLAYQLPEKAGGSESARTTAPTSSQLPTLSECLAMPEPGYGDGDDDTAAAIVARAGIQVGGLQQTDHLYYASQFGAAEGSLSSLQHQMAKPEQWADSSSLHSMLGSVIQSEADQQQEQDSGLQLVHLLLACADLVSKGDQPSALRHLHLLRRVASPLGDSMQRVASYFADALAARLALACPSSVVSPGGAPFPFPPSPDTLKIYQILYQACPYIKFAHFTANQAIFEAFQGEDRVHVVDLDILQGYQWPAFLQALAARPGGPPTLRLTGVGHPAAAVRETGRHLASLAASLRVPFEFHAAVADKLERLRPAALQRRVGEALAVNAVNRLHRVPGAHLAPLLSMIRDQAPKIMTLVEQEAGHNGPYFLGRFLEALHYYSAIFDSLDATFPADSAPRMKVEQCLLAPEIRNVVACEGAERVARHERLDRWRRIMEGRGFEAVPLSPAAVGQSQVLLGLYGAGDGYRLNEDKGCLLLGWQDRAIIGASAWRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I645</accession>
    <name>A0A0Q3I645_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYB-CC type transcription factor LHEQLE-containing domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_3g55120v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I645"/>
    <dbReference type="STRING" id="15368.A0A0Q3I645"/>
    <dbReference type="EnsemblPlants" id="KQK01315">
        <property type="protein sequence ID" value="KQK01315"/>
        <property type="gene ID" value="BRADI_3g55120v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01315">
        <property type="protein sequence ID" value="KQK01315"/>
        <property type="gene ID" value="BRADI_3g55120v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3I645"/>
    <dbReference type="OrthoDB" id="551907at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3I645">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="Myb_CC_LHEQLE"/>
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        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
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        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF80">
        <property type="entry name" value="HTH MYB-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
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    </dbReference>
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    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MYB-CC type transcription factor LHEQLE-containing" evidence="5">
        <location>
            <begin position="340"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK01315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="425" mass="47657" checksum="7F831F16FE83FF45" modified="2016-01-20" version="1">MYTAILDTSTMSQFRDGEVPWYLSSDITVLPALPEVMVPSFPQYSASYIERGLCRSSITPLNTFLPASSGLQSTYMSASNFTSDLCINNGLPNEKLSSGSHIIAISGFDTSLSSTHSSYKDDPSSLRMLYPKVSEEIYWVQEPVPGVFDYPASLNVSDQRNLVVSQEMQDIITLDHDTHLAKQKEWFSSESSGKFLENSGCGGSVLKAVDTRSTAPPNYTYFHMQNNVSSHFNVDELCSDNFPSSDTAPTKSRMRWTTELHELFVGAIIKLGGSEKATPKAVQKIMKVEGLTIYHFSFIRFHLCTNLEGYLCLHNFKHISKYPKGGVMPWVCARDLEDMDTSEGLRTQIGLQKQLHEQLEIQRKLQLQVEEHSKYLEMAIAKQGESLKQLGALPVFENSRTQVLDHIKACEDQTVDFSGREALRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IBZ5</accession>
    <name>A0A0Q3IBZ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100837554</name>
        <name evidence="4" type="ORF">BRADI_2g07801v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754539.1">
        <property type="nucleotide sequence ID" value="XM_014899053.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IBZ5"/>
    <dbReference type="FunCoup" id="A0A0Q3IBZ5">
        <property type="interactions" value="1179"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3IBZ5"/>
    <dbReference type="EnsemblPlants" id="KQK03427">
        <property type="protein sequence ID" value="KQK03427"/>
        <property type="gene ID" value="BRADI_2g07801v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837554"/>
    <dbReference type="Gramene" id="KQK03427">
        <property type="protein sequence ID" value="KQK03427"/>
        <property type="gene ID" value="BRADI_2g07801v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837554"/>
    <dbReference type="OrthoDB" id="277832at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IBZ5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019510">
        <property type="entry name" value="AKAP7-like_phosphoesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009210">
        <property type="entry name" value="ASCC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13360">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13360:SF1">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10469">
        <property type="entry name" value="AKAP7_NLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="136"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK03427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="481" mass="53421" checksum="C791F3EB35F50F18" modified="2016-01-20" version="1">MFAHSSLLRFSRLARRPLCAAGSPLLRYLQGSVGQNNLHSFAMERAQGVSGNAAKHRKTNSPVQRWRPVSTEASPQKADLDEISSSGMKQVLETSVTDSENLVSDETTTNVVIEVTTNNASSSENSSTFGSSGTKVVIKDTVEVDVPLMRFLKGKGGSIQKQIEQETGVKIIFPSSKEETLVVLEGQNAESIRKASERIAKVLEEAVQSPMLDYSHFVSLPLAIHPDLVEKLNNFQRSILGASSFNVDSDKDESLSEGSVDETDEVESPSVSTKLLVQEEKSIMVKRDNRGSQPEFGIDKSIFIKPKTFHLTVLMLKLWNKDRLAQASDVLQSISSQVNEALENRPVSIQLRGLTCMKGSPARARVVYAPVLEVGGEGRLVHACKVITEAFVKSGLVLERDARQELRLHATIMNVRHRKSKKRDRRNDSIDARNIFRQYGEHEWGEYPIPAVHLSQRFKFDESGYYHCCSSIPLPEATQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ITR8</accession>
    <name>A0A0Q3ITR8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100824896</name>
        <name evidence="9" type="ORF">BRADI_2g10017v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315827.1">
        <property type="nucleotide sequence ID" value="XM_024460059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ITR8"/>
    <dbReference type="EnsemblPlants" id="KQK03820">
        <property type="protein sequence ID" value="KQK03820"/>
        <property type="gene ID" value="BRADI_2g10017v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT70325">
        <property type="protein sequence ID" value="PNT70325"/>
        <property type="gene ID" value="BRADI_2g10017v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824896"/>
    <dbReference type="Gramene" id="KQK03820">
        <property type="protein sequence ID" value="KQK03820"/>
        <property type="gene ID" value="BRADI_2g10017v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70325">
        <property type="protein sequence ID" value="PNT70325"/>
        <property type="gene ID" value="BRADI_2g10017v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824896"/>
    <dbReference type="OrthoDB" id="10262656at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3ITR8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF109">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="50"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK03820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="492" mass="53579" checksum="D17E5441C280E4FE" modified="2016-01-20" version="1">MSDDAPGGGGSSSGGGAVEPLLKRSRYHPNCPGCRVDRRKEEREGFPFTELSCMWLVTICSSLPLHSLFPFLYFMIRDLHIAKKEEDIGFYAGFVGGSYMFGRALSSVIWGVVADKYGRKPVLVITLFSVIIFNTLFGLSSSYWMALATRGLLGLFSGVLGPIKAYATEVSRKEHSHLALSLISSSRGIGLIVGPAIAGYLAQPADKYPGIFSQNSIFGRFPYFLPCLCISLLAIAALVACFWLPETLHKHKDTISNNSVEAAEESLSDPNAEETFDGSLGLFTHGPLISAITVYCIFSLQDMAYAEVFSLWAVSDRKYGGLSFSSQDVGSILSTSGLFLLIYQILIFPWVAKSIEPIALVRTIAILTIPLLSSYSFMPALSGFSLQLVVNCASFLKNAFSVTTIIVFNILMNDAVSQNLRASVNGFSVTLMSIFKAIGPAIAGVIFSWAQRRQTAIFLPGDHLVFFMLNVVTVVGITLTFRPFFARSGTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J1P3</accession>
    <name>A0A0Q3J1P3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100846916</name>
        <name evidence="3" type="ORF">BRADI_2g27160v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568574.2">
        <property type="nucleotide sequence ID" value="XM_003568526.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A0Q3J1P3">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3J1P3"/>
    <dbReference type="EnsemblPlants" id="KQK06588">
        <property type="protein sequence ID" value="KQK06588"/>
        <property type="gene ID" value="BRADI_2g27160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846916"/>
    <dbReference type="Gramene" id="KQK06588">
        <property type="protein sequence ID" value="KQK06588"/>
        <property type="gene ID" value="BRADI_2g27160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846916"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3J1P3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45181">
        <property type="entry name" value="HEAT SHOCK PROTEIN DNAJ WITH TETRATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45181:SF4">
        <property type="entry name" value="HEAT SHOCK PROTEIN DNAJ WITH TETRATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="1343"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="857"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1435"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="872"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1461"/>
            <end position="1475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK06588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1486" mass="160239" checksum="77B1FE65CF52C681" modified="2016-01-20" version="1">MPSAVAPSTPRLNPSWPHPPPAAPMTSPHPPFAAPMASPHLGLGLAPPPLPTDPSVAPPPRRAPRLAKRRHAATTSRSRAQPSPSAPWNPFGGGGDTDGSGQNVNGGLGSVNGGGGSGFGKGQTGGVVFGSAPAGKQQSSQPPPASSSDAPFVFGSVRDSLPRFVEGSSATSKLRDRMGKLNLRSPDGYTSSSVHVTEANAMQEKLAQVSIGNEAPVRIHSEATTVTPTVFMSGSGHADSTDDVASSSSKNASSAVNGTDDTNALPEKITPLNIGSGTPFQNMKYGGGDSHQTEVFGFGGGRPAAAGTVYGNNSGNTSDKGKDFFSPANNNASTSANVATSLPPESTYNLNVRGGVRHKMKTDNANCLPETFVFGRNGSTSSASEQSARVVVDDGGNFVSGPSTDTRTSDQGSMESSLQEEMTKLNIQYRIPSKSMKDEAASLQPETFVFRSDASSSFFSAQVPSFTNFQTNASSETKDSGKNLADDIIRKSTYAKSNNSNGYRTSNFVFGSGSSATAASEGAEEHTPHDINKLNINMEGPSVCIQVNDASIPDFSFQGKAEAMPGYGTVPRPKVQESCPFTRLNHSSIFSTFENVMPSFSLNPMDAGKETAPGESGAIKQDLPRCSRESLFGIDYIKSAYRDKKEAHKSTRKKKRPTRLKQHAQFNQVSQETCINGVSSDLAGDYSPMDCSPYQVAVEEVSREASVSSDQSIHIPDGGVPNQNTSCAEDDLVSATEHLVIDADLPTYEDEGRAANVDASESNCGSNFSSFDEEVNFCNASEPSFNNINVGANDEPKMCTTEAWFDGYECNVKGKTCEDDTSRKLHESGEPVNFQSSSENFSGLSFTFGASLYPDSTLSTEKRNTKRKSRTKGGQTPKPSAVQASVKTKASRDTKGVQFTPETSKTEHSSKEISAALETCDTWRTSGNQAYENGHFATAEGCYTRGINSISHHGTSGRCSRALTLCYSNRAATRMSLGRMREALEDCSIATSIDPTFLKAKVRAANCQLALGDLDGASRNYTACLKSDNAAGSDFKMFAEASDGLEKAKRVADMISQSRELLKKRTLPEATTALELICNALHISSHSDNLMEMKADALLTLRRYEEVIELCQETVHLAERNSVLVNGNGESNNSSVSEKAECSGSLWRPYLICKSYFLLGKLEEALDLLMRHELVTPVKDSDGSTSQKCLSSLSTSIRQLLSFKDAGNESFKARRYSEAVEQYSAALACNSDSRPFSAVCFCNRAAAYQALGQLTDAIADCSLAMVLDANYPKAISRRATLYEMIRDYGQSANDLRKLISLLQKQANKPGVSPKVLNTYSDLKQARARLLSVEDEAKKDAPLNLYLILGVEPSCSPLDIKKAYRKAALKHHPDKASQLLVRNENADDGFWRDVVKEVHADADHLFKMIGEAYNILSDPQKREEYDMEEELRNAARRTFKGRSTQSPEQHFRRQYDRGFSPRQWQSAGKSGASRSRWSGHGYSDDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J2S9</accession>
    <name>A0A0Q3J2S9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC104585218</name>
        <name evidence="24" type="ORF">BRADI_4g44901v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 27">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 27">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758357.1">
        <property type="nucleotide sequence ID" value="XM_014902871.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J2S9"/>
    <dbReference type="EnsemblPlants" id="KQJ92632">
        <property type="protein sequence ID" value="KQJ92632"/>
        <property type="gene ID" value="BRADI_4g44901v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104585218"/>
    <dbReference type="Gramene" id="KQJ92632">
        <property type="protein sequence ID" value="KQJ92632"/>
        <property type="gene ID" value="BRADI_4g44901v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104585218"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3J2S9">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000479">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000221">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000177">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase At1g17230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF123">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="737"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="801"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="830"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQJ92632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1083" mass="119445" checksum="2E4A1253B79C3A10" modified="2016-01-20" version="1">MSMLCSLISSFKQAHSLAMESLSPKLLASFTILIMATAATLVSVTAAVPPSLEGQAGALLAWKATLNNESQQALQSWANMSAPCNWRGIRCGMDKVQQQPVIVNISLPGMRLRGKLESLDFSALRTLTSLDLSHNELAGSIPWSIEVLVELHALLLQSNQIRGSIPPSLANLTKLRILELSNNQISSEIPRQIGNMSNLVTLNLSVNHLTGPIPPEIGHLEQLSILDLFDNNLSGSIPSSVGDLTKLTILYLDQNHLSGHIPPELGYLGNLEALALSNNTITGSIPRDLCNLTKLTTLYLWRSKLSGHIPPELGYLVNLEELSLQKNTLLGSIPNSLRNLTKLRILYLYTNKLSGYIPQQIGHLINLEELDLSENNLSGSIPNSLGDLKKLIVLNLFKNQISGKVPQEIGNLINLVNLDMSSNNLFGPLPSGLCAGGQLQRFVAMKNNLVGPLPTSLLICKSLVRVRLEQNQLEGDISKMGSYPNIVYIDISSNKLYGQLSHRWGECYKLTMLRASNNNINGAIPPSIGRLSRLRVFDVSSNKLDGEIPPEIGNLLALFNLSLGNNFLWGTIPHEVGFLTNLEYLDLSSNNLSGSIQGSIEHCCKLRFLKLSHNRLNGSIPIELGMLGNLQALLDLSDNSFKDKIPSQLGSLRMLEALNLSKNALNGSIPSSFLSLVSLLSIDMSYNQLEGSVPQNRFFEEAPIEWFLHNSKLCGVVKGLPACQITQSHGQDKKSKLFLLAIIPSVIFIILITAIVRILQCKRKKSNERSQIELQQTNLFAIWNFDGEDVYEKIMDATENFSDTHCIGIGGNGSVYRAQLPTGEIFAVKKIHAIEDSETFNREIDALLHIRHCNIAKLFGYCSAPRGRFLVYEYMDRGSLASYLKSEVTAIELDWARRLNIVRDVAHALSYMHHDCFAPIVHRDITSNNILLDLKFRACISDFGIAKILDVNAANCTKLAGTKGYLAPELAYTTRVTEKCDVYSFGVLVLELFMGHHPGDFLLSMATESTSLEDLLDTRLQLPEVGLASEIFKVIMIAIRCIEPNPSHRPTMQHVINMSSTTEGPDTTLDYLHTGIVTSVCWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J9E4</accession>
    <name>A0A0Q3J9E4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g16690v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J9E4"/>
    <dbReference type="EnsemblPlants" id="KQK14499">
        <property type="protein sequence ID" value="KQK14499"/>
        <property type="gene ID" value="BRADI_1g16690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14499">
        <property type="protein sequence ID" value="KQK14499"/>
        <property type="gene ID" value="BRADI_1g16690v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3J9E4"/>
    <dbReference type="OrthoDB" id="686549at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33127:SF85">
        <property type="entry name" value="OS11G0436500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33127">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="19"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="318" mass="34748" checksum="77B4E98AC464E3C6" modified="2016-01-20" version="1">MFSVAGKHPIAGGGMYSDELADKAFCPTPQGWVLVRDRLDATDTYLLDHHTGDKIRLPPLTIDPDRLIDCTCLLSHTRPATSPSCVLLVVQPDEPIIWYVHVNAGNGSNGWAWTRHEYDIGTQGNEACGFESILMDMAAACRGKFYFVTSPIDFGVLEFSPAPVFTAFVVTEDDDEGGYDSTETGRAFLVESDDELYMVKQLTSQDYNEALVYKLEESSTSSGQRQWRWRPVHDLGGRAFFVAPFNFGTSCLVAGDESGIQPNCCLTVFDVTDGTMQLCSIEQALSFNGGAGSEEVPPPATAKTMTWMLPTDPKAAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JT66</accession>
    <name>A0A0Q3JT66_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">trimethyltridecatetraene synthase</fullName>
            <ecNumber evidence="14">1.14.14.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BRADI_1g57000v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(6E,10E)-geranyllinalool + reduced [NADPH--hemoprotein reductase] + O2 = (3E,7E)-4,8,12-trimethyltrideca 1,3,7,11-tetraene + but-3-en-2-one + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13545"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:48058"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:74299"/>
            <dbReference type="ChEBI" id="CHEBI:74322"/>
            <dbReference type="EC" id="1.14.14.58"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13546"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.58" evidence="14"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JT66"/>
    <dbReference type="STRING" id="15368.A0A0Q3JT66"/>
    <dbReference type="EnsemblPlants" id="KQK20835">
        <property type="protein sequence ID" value="KQK20835"/>
        <property type="gene ID" value="BRADI_1g57000v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20835">
        <property type="protein sequence ID" value="KQK20835"/>
        <property type="gene ID" value="BRADI_1g57000v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JT66"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JT66">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080027">
        <property type="term" value="P:response to herbivore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20618">
        <property type="entry name" value="CYP71_clan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000097">
        <property type="entry name" value="Cytochrome P-450 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944:SF3">
        <property type="entry name" value="TRIMETHYLTRIDECATETRAENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15 16" id="KW-0349">Heme</keyword>
    <keyword evidence="10 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK20835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="519" mass="57041" checksum="5A38A1DA28715EE8" modified="2016-01-20" version="1">MALLPIIVFLVVTYIFSQRFSSYKPLNLPPGPRGWPVIGSLPLLAGPLPPHRALASLAARHGPLMHLRLGSFPTIVASTADAARLVLKTHDLSFADRPRTAAGQHAAYGYRGIVHTPYGAYWRMARRLCATELFSPRRVASFEPVRAQELRALARVLYELGLGAGHGDGGVPVAVREHVAGLTMRNALRMAVGEKWSACYGSAEGVEFRRALDEAFAVTGAVNSVGEWIPWLGRLDLQGHVRSMKRLSERFDRFLEKILDDHEQEHDRRPREAAPRDLVDVLLRLADEEEEGLTREGVKAFVQDIIAGGTESSAVTVEWAIAELLRRPESMAAATEELDRLVGRARWVAESDLPELPYLDAVVKETMRLHPVGPLLVPHMARERTVVAGGYEVPAGARVLVNAWAVGRDPASWPDRPDEFRPERFRLLDVDVDVRGQHFQLLPFGAGRRMCPAVGLAMKVVAGGLATLLQGFAWRLPDGVAPGDLSMEEFVGLSTRRKVPLVAVPVPRLPAHLYAAAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K3W8</accession>
    <name>A0A0Q3K3W8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1677 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g20325v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K3W8"/>
    <dbReference type="FunCoup" id="A0A0Q3K3W8">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3K3W8"/>
    <dbReference type="EnsemblPlants" id="KQK05489">
        <property type="protein sequence ID" value="KQK05489"/>
        <property type="gene ID" value="BRADI_2g20325v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT70944">
        <property type="protein sequence ID" value="PNT70944"/>
        <property type="gene ID" value="BRADI_2g20325v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05489">
        <property type="protein sequence ID" value="KQK05489"/>
        <property type="gene ID" value="BRADI_2g20325v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70944">
        <property type="protein sequence ID" value="PNT70944"/>
        <property type="gene ID" value="BRADI_2g20325v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K3W8"/>
    <dbReference type="OrthoDB" id="1911663at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012876">
        <property type="entry name" value="DUF1677_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33108">
        <property type="entry name" value="OS01G0745000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33108:SF36">
        <property type="entry name" value="OS05G0509800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07911">
        <property type="entry name" value="DUF1677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK05489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11063" checksum="59AA7D4E8FA56974" modified="2016-01-20" version="1">MDTESMRCACCDLKEECTQEYIKSVKDNFGGKWLCGLCSEAVGDELTRDRRGQEGVEEAIKAHMAFCRMTLSSPAVRVADGMKEMLRRRSRDKGSSSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K501</accession>
    <name>A0A0Q3K501_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g22695v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K501"/>
    <dbReference type="FunCoup" id="A0A0Q3K501">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3K501"/>
    <dbReference type="EnsemblPlants" id="KQK05817">
        <property type="protein sequence ID" value="KQK05817"/>
        <property type="gene ID" value="BRADI_2g22695v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05817">
        <property type="protein sequence ID" value="KQK05817"/>
        <property type="gene ID" value="BRADI_2g22695v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K501"/>
    <dbReference type="OrthoDB" id="645570at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011676">
        <property type="entry name" value="DUF1618"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33086">
        <property type="entry name" value="OS05G0468200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33086:SF5">
        <property type="entry name" value="OS05G0468400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07762">
        <property type="entry name" value="DUF1618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1618" evidence="1">
        <location>
            <begin position="269"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK05817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="466" mass="50609" checksum="2E644D7ED6C0CC98" modified="2016-01-20" version="1">MLLPLRGLSGRLRRSLSTGASRLPWALIYRISTADESARAVSLSLAAPPLASRVSIPKKAFGLDPIPATSSTGRDFSNAFGTGIPPPLLAHATAPGQASGPYSSADSKFVNVFGSGVLAASSDGFLLLSTFKNRARAWSMFVHQVPNAAPFEVLDHVYARSSCDPMSVSRFVCNPITGQLFRLPDIDGAENTYTAATGLLTEADGGHGPPRKYAAAQLSVVDGGHQFLLRRFSSDTGEWEELVLPSPLPPERRMYMNHEVLDFGGRLWWVDVSWGAVCVDPFSDRPELCPVEFPGGIKLPDQQGETEMGQLVKHRRMGVSDGRLRFVEVSKEQPFRIKSFTLDDESGCWKLEHQLSFSTLISDEGRRLKEAPLIGALDPLSTDLLYLTAPDVGKGFCFSIDMRWKRPITSSALGGGVHPSKCESSFLLPCVLPSFLGSSTIPGTNDVTKNKTLADVLVRSDRRKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K765</accession>
    <name>A0A0Q3K765_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC100832364</name>
        <name evidence="21" type="ORF">BRADI_1g54167v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">Bd21</strain>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K765"/>
    <dbReference type="EnsemblPlants" id="KQK20380">
        <property type="protein sequence ID" value="KQK20380"/>
        <property type="gene ID" value="BRADI_1g54167v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20380">
        <property type="protein sequence ID" value="KQK20380"/>
        <property type="gene ID" value="BRADI_1g54167v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="543442at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K765">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000051">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000811">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000517">
        <property type="entry name" value="Putative receptor kinase Lecrk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019825">
        <property type="entry name" value="Lectin_legB_Mn/Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00307">
        <property type="entry name" value="LECTIN_LEGUME_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 19" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044546038" description="non-specific serine/threonine protein kinase" evidence="19">
        <location>
            <begin position="22"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="355"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQK20380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="687" mass="75821" checksum="8F174A51E27769D4" modified="2016-01-20" version="1">MMKLMTFFLLSFSFQLSAVLCAGDGFQFAFPGLAGANLTLDGNASVTRDGLLVLTTLKTNLKGHAFYPTPLQFRRSPGGKVRSFSSAFVFAIVSDYTDFSAHGMAFVVCPSPESFSSALPAGYLALLNVQNNGNATNHLLAVELDTTQNTDFQDIDANHVGIDINSLHSLQSHTAGYYPDHGDGDSGGFENLTLFSRDAMQVWVSYEGAGAGQINVTLAPIGAPKPEKPLLSAAYDLSTVLTDQAYIGFSSSTGGINSRHYVLGWSFAMDEGGPAPAIDIARLPKLPRFGPKPRSKVLEIVLPIATAAIIITVGTVVALLVLRRLRYAQVLEDWEVEFGPHRFSYKDLYHATKGFKNKQLLGAGGFGKVYKGVLRKSKLEVAVKKISHESRQGMKEFIAEVVSIGRLRNRNLVQLLGYCRRKGELLLVYEFMPNGSLDKYLHLGEDMPVLLDWPKRFHVIRGVASGLLYIHDKWEKVVIHRDIKASNVLLDSEMNGRLGDFGLSRLYDHGTDPQTTHVVGTMGYLAPELIRTGKASTLTDVFAFGAFLLEITCGKRPVNDGLQGDYEMLVDWVLENLHKGSLTETVDARLEGDYDADEVCLVLKLGLLCSHPFTNIRPSMRTKGSIHTPCLIHLQLELALAPYQTYQGVRYLYSWIKENTVHMPTNANIYERRQCCTTSTRKPLNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KCX3</accession>
    <name>A0A0Q3KCX3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100826339</name>
        <name evidence="14" type="ORF">BRADI_2g44117v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KCX3"/>
    <dbReference type="STRING" id="15368.A0A0Q3KCX3"/>
    <dbReference type="EnsemblPlants" id="KQK08822">
        <property type="protein sequence ID" value="KQK08822"/>
        <property type="gene ID" value="BRADI_2g44117v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK08825">
        <property type="protein sequence ID" value="KQK08825"/>
        <property type="gene ID" value="BRADI_2g44117v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08822">
        <property type="protein sequence ID" value="KQK08822"/>
        <property type="gene ID" value="BRADI_2g44117v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08825">
        <property type="protein sequence ID" value="KQK08825"/>
        <property type="gene ID" value="BRADI_2g44117v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KCX3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF271">
        <property type="entry name" value="OS09G0403300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK08825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="59631" checksum="0D90B1800CE25C63" modified="2016-01-20" version="1">MGGGTSAAGVALLRDASPWTLLAGAAAVAILCCAVQILEWAWWAPRRMDRSLRAQGLRGTQYRFFRGDLKEEQRLMAAALSRPVPMDRAHDIVSRVAPLLHRVMEEHGKLSFTWFGPCPRIIITDPELVREVLSNKFGHFEKTKLARLSKLLVGGLAVLDGEKWIKHRRIMNPAFHAEKLKRMLPAFSASCSELIGKWENLFAVSHGGIQLDVWSEFQNLSGDVISRAAFGVSHQEGCRIFLLQAEQAERLVQSFWTCYIPGYSLLPTENNRRMKAINKEIKAILRGIIEKRQKSMQSGETNEDDLLGLLLESNMDYSDSDGKLSKGMTVEDVIGECKLFYFAGMETTAVLLTWTVVVLSMHPEWQDRAREEVLHVFGQSKPDLNGLNRLKVVTMILNEVLRLYPPVVQINRRTNKKIELRGVMYPQGVMLALPLICIHRDPSVWGNDADKFNPGRFSEGVPKACRETGAFFPFSWGPRTCIGQNFALLEAKIRGPIPNGKKVPCSFDALETPSENLSGLNLLASFPQISGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PR27</accession>
    <name>A0A0Q3PR27_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106866775</name>
        <name evidence="2" type="ORF">BRADI_4g40850v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="2">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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</entry>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_2g42643v3"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="35"/>
            <end position="78"/>
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    </feature>
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        <source>
            <dbReference type="EMBL" id="KQK08578.1"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="78" mass="7623" checksum="05E5CDD3F616EEF0" modified="2016-01-20" version="1">MSDFVASGESEAAVSCLSQDSVISVSETTIEIAPASVSDNGYDGDGEESVGFGGGADSDAEGLGEEEATAEGLGTRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
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            <location evidence="1">Secreted</location>
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        <property type="gene ID" value="BRADI_1g38859v3"/>
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        <property type="gene ID" value="BRADI_1g38859v3"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK18114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="83" mass="8671" checksum="132236D09622C893" modified="2016-01-20" version="1">MKLLMCLCVLVVLAVGVASSPVPLSGDRPLTLLGRRGLQDVVVIGSSPPAPASTAGTWPRDTPDISYDRSKRLSPGGSNPQHH</sequence>
</entry>
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the E2F/DP family.</text>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGH6</accession>
    <name>A0A2K2CGH6_BRADI</name>
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        </submittedName>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="PNT61132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="93" mass="10124" checksum="C0FCC302ED563BAD" modified="2018-03-28" version="1">MPPPCLTLRSRRDGIKPSSALISAWNDQEAATGGAGSWLGTLEGARWSVAETCDFSASWDQLASHQSSGNLENKGFGSLDWGIRRKNRKFSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGR4</accession>
    <name>A0A2K2CGR4_BRADI</name>
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    </protein>
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    <organism evidence="1">
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT61219">
        <property type="protein sequence ID" value="PNT61219"/>
        <property type="gene ID" value="BRADI_5g12212v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT61219"/>
        <property type="gene ID" value="BRADI_5g12212v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CGR4"/>
    <dbReference type="OrthoDB" id="800068at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT61219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="90" mass="10359" checksum="6A49CECE422326C7" modified="2018-03-28" version="1">MVARLTPDQKVACSIHVGFNTPNLIRISFVFQFITQRHQGFSHSGVTELFLLGCQWTVKIWISSPWIGFSIMDIQAWIPVNSWSGHEYHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHQ5</accession>
    <name>A0A2K2CHQ5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g16775v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHQ5"/>
    <dbReference type="EnsemblPlants" id="PNT61562">
        <property type="protein sequence ID" value="PNT61562"/>
        <property type="gene ID" value="BRADI_5g16775v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61562">
        <property type="protein sequence ID" value="PNT61562"/>
        <property type="gene ID" value="BRADI_5g16775v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CHQ5"/>
    <dbReference type="OrthoDB" id="691180at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT61562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="80" mass="8913" checksum="11727DCE60E93C13" modified="2018-03-28" version="1" fragment="single">CCKVQFQTENSLQGKELGKVVASISLLVLWETWKERNRRMFQNKLRPKEGVLSIIKEEAAVWKQAGAGIGVLCSGPDDVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CJJ7</accession>
    <name>A0A2K2CJJ7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Pre-mRNA cleavage factor Im 25 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100834278</name>
        <name evidence="8" type="ORF">BRADI_5g26810v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family. CPSF5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT62185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751655.1">
        <property type="nucleotide sequence ID" value="XM_014896169.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751656.1">
        <property type="nucleotide sequence ID" value="XM_014896170.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751657.1">
        <property type="nucleotide sequence ID" value="XM_014896171.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751658.1">
        <property type="nucleotide sequence ID" value="XM_014896172.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751659.1">
        <property type="nucleotide sequence ID" value="XM_014896173.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751660.1">
        <property type="nucleotide sequence ID" value="XM_014896174.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311436.1">
        <property type="nucleotide sequence ID" value="XM_024455668.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311437.1">
        <property type="nucleotide sequence ID" value="XM_024455669.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311438.1">
        <property type="nucleotide sequence ID" value="XM_024455670.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311439.1">
        <property type="nucleotide sequence ID" value="XM_024455671.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311440.1">
        <property type="nucleotide sequence ID" value="XM_024455672.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311441.1">
        <property type="nucleotide sequence ID" value="XM_024455673.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311442.1">
        <property type="nucleotide sequence ID" value="XM_024455674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CJJ7"/>
    <dbReference type="STRING" id="15368.A0A2K2CJJ7"/>
    <dbReference type="EnsemblPlants" id="PNT62185">
        <property type="protein sequence ID" value="PNT62185"/>
        <property type="gene ID" value="BRADI_5g26810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834278"/>
    <dbReference type="Gramene" id="PNT62185">
        <property type="protein sequence ID" value="PNT62185"/>
        <property type="gene ID" value="BRADI_5g26810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834278"/>
    <dbReference type="OrthoDB" id="277288at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CJJ7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18871">
        <property type="entry name" value="NUDIX_Cfim25_Nudt21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000020">
        <property type="entry name" value="Pre-mRNA cleavage factor Im subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016706">
        <property type="entry name" value="Cleav_polyA_spec_factor_su5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13047">
        <property type="entry name" value="PRE-MRNA CLEAVAGE FACTOR IM, 25KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13869">
        <property type="entry name" value="NUDIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT62185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="32651" checksum="05CEF5FB7B8657F0" modified="2018-03-28" version="1">MRGSLAHVRPLSPPFVLSVNTASGTVAHTSIYFARLREDGTLLPKPSPSRKPTTSLFLLLAAASSGDHRSPRRKRKSESSARRRKEMVSASSSSVVNVYPLANYTFGTKEPKMEKDTSVADRLARMKVNYMKEGMRTSVEAIMLVQEHNHPHVLLLQIGNTFCKLPGGRLKPGENEIDGLKRKLCSKLGVNSPSFPPNWQVGECVGVWWRPNFDNVMYPYCPPHITKPKECKKLFIVHLTEREYFAIPRKLKLRAVPLFEIYDNVQRFGPVISTIPLQLSRFQFNMVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CM79</accession>
    <name>A0A2K2CM79_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Laccase</fullName>
            <ecNumber evidence="5 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100842590</name>
        <name evidence="17" type="ORF">BRADI_4g11840v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Secreted</location>
            <location evidence="3 13">Extracellular space</location>
            <location evidence="3 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="5 13"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318715.1">
        <property type="nucleotide sequence ID" value="XM_024462947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CM79"/>
    <dbReference type="EnsemblPlants" id="PNT63130">
        <property type="protein sequence ID" value="PNT63130"/>
        <property type="gene ID" value="BRADI_4g11840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842590"/>
    <dbReference type="Gramene" id="PNT63130">
        <property type="protein sequence ID" value="PNT63130"/>
        <property type="gene ID" value="BRADI_4g11840v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="590181at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CM79">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF356">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="11 13" id="KW-0186">Copper</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043074896" description="Laccase" evidence="13">
        <location>
            <begin position="26"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="35"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="162"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="440"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="PNT63130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="606" mass="66218" checksum="4A351B1E5D044C8B" modified="2018-03-28" version="1">MAAMALSATSVAVFFFLAVLSGGDAAVVEHTFVVSQVKMNRACRGDTLVTVVNGQLPGPAIEVTEGDSVVVHLVNKSPYGLTIHWHGVKQRLNCWADGVDMVTQCPIQPGRNFTYRFNVAGQEGTLWWHAHVASFRATVHGALIIRPRSGVTSYPFPEPHKEIPIFIGEWWEVDLVKLDTTLGDGVDYNPVNTTINGKLGDLYNCSVHLCDSGCEGALEDNFIIEVEQGKTYLLRIVNAALFSEYYLKIAGHRFTVVAADANYVKPYTTDIIAIAPGESVDAIVLADAPPGKYYMVALGNQQPPPDIQIPPLSSRVLVKYNYNPSKEQALTLGASVMAPEMPDQHNTIASFYFHGNMTGSFAHLPVPVHVDEHLFISLALGVICRGGHVPSPSLPCSKLNGSILAATMNNISFEFPSNVSLLEAHYRGNMSNAGMLHKLPDKPPIVYNFTDRAMISEGSEEAALESTLIATTVRRFRFNTTVEIVFQSTTVLQSDSNPMHLHGHDFFVLAQGLGNYNAERDVGRYNLVDPPVRNTVLVPSSGWAAIRFVTDNPGVWFLHCHYGFHMSIGMAVVFEVDNGQTLNTTLAPPPADLPICEQHDSSVAYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMC3</accession>
    <name>A0A2K2CMC3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box protein AT5G49610-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g12755v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CMC3"/>
    <dbReference type="FunCoup" id="A0A2K2CMC3">
        <property type="interactions" value="813"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63184">
        <property type="protein sequence ID" value="PNT63184"/>
        <property type="gene ID" value="BRADI_4g12755v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63184">
        <property type="protein sequence ID" value="PNT63184"/>
        <property type="gene ID" value="BRADI_4g12755v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CMC3"/>
    <dbReference type="OrthoDB" id="592687at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CMC3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056594">
        <property type="entry name" value="AT5G49610-like_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33207">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23635">
        <property type="entry name" value="Beta-prop_AT5G49610-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box protein AT5G49610-like beta-propeller" evidence="1">
        <location>
            <begin position="184"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="F-box protein AT5G49610-like beta-propeller" evidence="1">
        <location>
            <begin position="272"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="465" mass="52159" checksum="0FBD853361E74410" modified="2018-03-28" version="1">MGLRVDVRAGLGSRCRTARGELGPSQTGIHPGTGLLPAVRIEPPTAVGACAPTNPPSPLAIPDRPTMDFLPSPAAAAALVSKVLDDDNLLRVGLPTTLARAALVCKRWFHNAANRAFLRQFRQRHPPRLLGLYVNGVFFTDTPESAKVLPRFVPLLTQTQEPELASVLRRMATLSLDTINRIMIEQCRNDSVFISRFQNHKYTYEVHNPLFPDRGVVVLPAPTRAMTDDSHKPTFSCFLSTGGDGCLSYMWMAMEINVIVGRSTAKVHMLPKPLLINYRIYMAGGTCNDILVLDLASSSFFTIQLPEGVEWSSGYKYGMGFHSRDAVFSRADDSGVYLIDLKELQFRIWLHKDDNWLLVDTICLRQMCAAFQMSDVMVEDEHTTAVSITQVGDNAEFVFLQMGPYTLYLDIRRRVLRKVYDEMTEKDRYVGHIHPCMMIWPPAFSVLKDDSTRFVFGLWVITALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMN9</accession>
    <name>A0A2K2CMN9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g13807v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CMN9"/>
    <dbReference type="EnsemblPlants" id="PNT63286">
        <property type="protein sequence ID" value="PNT63286"/>
        <property type="gene ID" value="BRADI_4g13807v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63286">
        <property type="protein sequence ID" value="PNT63286"/>
        <property type="gene ID" value="BRADI_4g13807v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CMN9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33086:SF10">
        <property type="entry name" value="DUF1618 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33086">
        <property type="entry name" value="OS05G0468200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT63286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="45277" checksum="623443F972EFAFEF" modified="2018-03-28" version="1" fragment="single">RCWTWYLLSKTAAAAGPAPGPAVAFASPPSISRLCVPQHLAKHSCGLPLPDPDDDVVRLLAVQACCASQNGLLLFNVQDIRAAAPILARQGAEQGGNHVRRFTGLADPGQLPGMTHYVFNPCNRDFILAGFKLGLLTQADAAGGFGPPDRYAVAEMEAHLGLMLRFLSETGEWEIVQGSPCQLPAGRRLLPNQEAVAWSGRLWWVDLAFGAVCAEPFSDRPEPHFVQLPSGSVLPADMGEKAFREALRRGTHLPDAEGNVSQVEPFVLSSFKLDTDCGGWTLEHRVALSRLWADGGHPWLPLQGGTTPLISALDMAESNVVYISVGKHIVGVDMHKGEVTGDCPAGGGHPVLRCIITPWLATTRIPGKKDVMKKDTLADVLVRSDRCGEMKVPTDASLLTHSYYLSYNHIFSTYISYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CNG7</accession>
    <name>A0A2K2CNG7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g17885v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CNG7"/>
    <dbReference type="EnsemblPlants" id="PNT63591">
        <property type="protein sequence ID" value="PNT63591"/>
        <property type="gene ID" value="BRADI_4g17885v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63591">
        <property type="protein sequence ID" value="PNT63591"/>
        <property type="gene ID" value="BRADI_4g17885v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CNG7"/>
    <dbReference type="OrthoDB" id="684023at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF5">
        <property type="entry name" value="OS05G0421675 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="147"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="316" mass="35801" checksum="B5031F718C3258EF" modified="2018-03-28" version="1">MKLESGDQDLSCQILQIKYLGEGGFFQSSSARGRHSFGKACMLLSNGLAQGVLIRLAMVVGFASARMFGVDEVPLSVHFFRLFRCCEQQGETVAEVLRDEDIHLSFRRSFGPEEQVEWEELCLILERVQLSDSCDQAYWCLTKNKRYATQSLYSALLHSGVRNLEIMDLWKASIPLKHKIFVWLGIQGRIQVTEELAKKGWPGERGCKLCGEPETADHVLLLCPIASFVWVILRDSFGWSAAPQSFGDFRALCLLKEGPSRHKWCVQGTGVSYWKTLLHEKRREPTMKMVAKVLEVLQLCVKEEKNRKLAMLVPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CPL2</accession>
    <name>A0A2K2CPL2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g22805v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CPL2"/>
    <dbReference type="EnsemblPlants" id="PNT63962">
        <property type="protein sequence ID" value="PNT63962"/>
        <property type="gene ID" value="BRADI_4g22805v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63962">
        <property type="protein sequence ID" value="PNT63962"/>
        <property type="gene ID" value="BRADI_4g22805v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CPL2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036319171" description="Secreted protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="10997" checksum="42FC69B466770097" modified="2018-03-28" version="1">MQALRSLSLHLPPLCLTLLLSPSSPCAGATPPFPSKRSGRRLQPPSHVPWRGGSCSVAVLPRVTVPDPMQPSLLPNLITLTAFRACPGTDPMRGTRQAMYVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CTV9</accession>
    <name>A0A2K2CTV9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100826602</name>
        <name evidence="13" type="ORF">BRADI_4g42860v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757510.1">
        <property type="nucleotide sequence ID" value="XM_014902024.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CTV9"/>
    <dbReference type="FunCoup" id="A0A2K2CTV9">
        <property type="interactions" value="389"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CTV9"/>
    <dbReference type="EnsemblPlants" id="PNT65463">
        <property type="protein sequence ID" value="PNT65463"/>
        <property type="gene ID" value="BRADI_4g42860v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT65464">
        <property type="protein sequence ID" value="PNT65464"/>
        <property type="gene ID" value="BRADI_4g42860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826602"/>
    <dbReference type="Gramene" id="PNT65463">
        <property type="protein sequence ID" value="PNT65463"/>
        <property type="gene ID" value="BRADI_4g42860v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65464">
        <property type="protein sequence ID" value="PNT65464"/>
        <property type="gene ID" value="BRADI_4g42860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826602"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CTV9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990538">
        <property type="term" value="F:xylan O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF213">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="12">
        <location>
            <begin position="125"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="11">
        <location>
            <begin position="179"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="PNT65464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="491" mass="54427" checksum="DB5AA356DC28B5FD" modified="2018-03-28" version="1">MVGSPTKRSAARRVMAARRPLEKVGMAGLAAAAAAAAALLLLFVCATASLRCSAAVGYALAAPQKLWWSGGVSIAAEASSSSAAERPRVDVRAAAAAGETEQVVGPVAAAERAQAVVPVAAVGAEECDLFDGEWAWDDGRPLYESRDCPFLDTGFRCSENGRPDSSYAKWRWRPSRCDLPRFDAKSMLEKLRNRRVVFVGDSIGRNQWESLLCMLSSAVPDKSSIYEIHGSPITKHMGFLIFKFSDYNCTVEYYRSPFIVLQGRVPAGAPKVVKYTVRVDVMDWMSGRGKWRDADVLIFNTGHWWNYEKTIRGGTYFQEGDEVKMEMTVHDAYQRSIQTLFDWLHKEVNTSKTQVIFRTYAPVHFRGGDWRTGGSCHLETLPDTTPFKSLEQWADLLQPVHNVLGSSINPKLPGLDILNVTQMTARRKDGHLSVYLSPSGPVPLHRQDCSHWCLPGVPDTWNELLYAVFMKRQAMMDQNVSLAGPRTLSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CV51</accession>
    <name>A0A2K2CV51_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_3g04198v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CV51"/>
    <dbReference type="STRING" id="15368.A0A2K2CV51"/>
    <dbReference type="EnsemblPlants" id="PNT65909">
        <property type="protein sequence ID" value="PNT65909"/>
        <property type="gene ID" value="BRADI_3g04198v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65909">
        <property type="protein sequence ID" value="PNT65909"/>
        <property type="gene ID" value="BRADI_3g04198v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CV51"/>
    <dbReference type="OrthoDB" id="647974at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000394">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000403">
        <property type="entry name" value="Receptor-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF95">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="534"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="563"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT65909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="781" mass="85958" checksum="F0EAE9B408F5848D" modified="2018-03-28" version="1">MLVSPENLADTPFFHPDRPQEIEHAFPWPRSCAADLLCLSYSSCTPQEKGFLLQFINGLSHDGGLATSWQDVTDCCKWEGITCRQANTVTDVLLVSKGLEGYISESLGNLTGLKHLNLSHNLLSGGLPLGLVSSHSITVLDVSFNLLNGALQELPTSISAQPLQVLKISSNLFTGEFPSTIWKAMENLILLNASNNSFSGGSIPPGIGDCSKLKVLRAGYNNLSGTIPDELFNTTLLEYLSFPNNNLHGALGEARIINLSYLETLDLGGNKFNGEIPDSIGQLKKLKEFHLNNNKMSDLYFNNFNGIVPESIYSCSNLTALRLAGNKLHGQLSPQIGNLKDLTFMSLSHNSFQNITNALHILQSCRKLTTLLIAFNFIGETIPEDENFDGLENLQVLGPSLQYRVPIALRKLLNLSKNKLTGEIPLEIGQLKALLALNFSYNRLTGQIPQSICNLTNLQVLDLSSNNLTAFNVSNNGLEGPIPSGGQFNAFQNSSFDGNPKLSEQTLVLMRMPHGKGEENKLKFTDILKATNNFDKENIIGCGGYGLVYKAELPDGSKLAIKKLNGEICLMEREFSAEVDALSMAPHENLVPLLGYSIQGNSRLLIYSYMENGSLDDWLHNRDDNASSFLDWPTRLKIAQGTSLGISYIHDVCNPQIVHRNIKSSYIPPEYGQAWVATLRGDMYSLGVVLLELLTGMRPLPVLSKSKELVPWVLDMRSQGKLVEVLDPTLRGTGYEDQMLKMLEAACKCIDHNQFMRPTIMEVVSCLASIDADSQMQNAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CVB0</accession>
    <name>A0A2K2CVB0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100825821</name>
        <name evidence="8" type="ORF">BRADI_3g04981v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of a sulfur ion from a donor to cyanide or to other thiol compounds. Substrate preference is 3-mercaptopyruvate &gt; thiosulfate. Involved in embryo and seed development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2-oxo-3-sulfanylpropanoate + [thioredoxin]-dithiol = [thioredoxin]-disulfide + hydrogen sulfide + pyruvate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21740"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57678"/>
            <dbReference type="EC" id="2.8.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>thiosulfate + hydrogen cyanide = thiocyanate + sulfite + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16881"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:18022"/>
            <dbReference type="ChEBI" id="CHEBI:18407"/>
            <dbReference type="ChEBI" id="CHEBI:33542"/>
            <dbReference type="EC" id="2.8.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569950.1">
        <property type="nucleotide sequence ID" value="XM_003569902.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CVB0"/>
    <dbReference type="FunCoup" id="A0A2K2CVB0">
        <property type="interactions" value="1175"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CVB0"/>
    <dbReference type="EnsemblPlants" id="PNT65964">
        <property type="protein sequence ID" value="PNT65964"/>
        <property type="gene ID" value="BRADI_3g04981v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825821"/>
    <dbReference type="Gramene" id="PNT65964">
        <property type="protein sequence ID" value="PNT65964"/>
        <property type="gene ID" value="BRADI_3g04981v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825821"/>
    <dbReference type="OrthoDB" id="270167at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CVB0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016784">
        <property type="term" value="F:3-mercaptopyruvate sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000001">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000019">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008557">
        <property type="entry name" value="PRK11493.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF12">
        <property type="entry name" value="OS02G0167100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="7">
        <location>
            <begin position="18"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="7">
        <location>
            <begin position="186"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT65964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="307" mass="33985" checksum="6C8E8D7F7131E748" modified="2018-03-28" version="1">MAQDDPVVSAQWLQRHLGQPDIKVLDASWHMPQESRDPWQEYKVAHIPGALFFDIDGIADRMTDLPHMLPSAEAFAAAVSALGIRNHDKVIVYDSKGFYSAPRVWWMFRILGHNEVWVLDGGLPQWQASGYNLGSNCPEDAILKSKAANTAVETTYNGELESAATFQTEFQPQLLWTLEKVKHNVAAQTHQVVDARSRGRFDGIEPEPRKGVRSGHIPGTKNVPFPEMFDGAPKLLPADELSKKFEQAGISLDHPIVVTCASGVTACIVALGLYRVGKHDIPVYDGSWTEWEAQPDIDYPKVTSTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D100</accession>
    <name>A0A2K2D100_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100846420</name>
        <name evidence="2" type="ORF">BRADI_3g34127v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D100"/>
    <dbReference type="STRING" id="15368.A0A2K2D100"/>
    <dbReference type="EnsemblPlants" id="PNT67950">
        <property type="protein sequence ID" value="PNT67950"/>
        <property type="gene ID" value="BRADI_3g34127v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67950">
        <property type="protein sequence ID" value="PNT67950"/>
        <property type="gene ID" value="BRADI_3g34127v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="201709at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042462">
        <property type="entry name" value="ARMC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46263">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46263:SF1">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="128" mass="13576" checksum="7221C22941BB9D5B" modified="2018-03-28" version="1">MFTNAQLQVERTGRGGTLREQYLQLNILELFLDCITEPNEWLVEFGVGGICNSCADPANASVITQCGGIPLVVQCLSSPVKNTVYEGREDREPTMIGAQETATGTSAPPLGASAVASDQSGEEIIVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D1K6</accession>
    <name>A0A2K2D1K6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g36465v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D1K6"/>
    <dbReference type="STRING" id="15368.A0A2K2D1K6"/>
    <dbReference type="EnsemblPlants" id="PNT68132">
        <property type="protein sequence ID" value="PNT68132"/>
        <property type="gene ID" value="BRADI_3g36465v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68133">
        <property type="protein sequence ID" value="PNT68133"/>
        <property type="gene ID" value="BRADI_3g36465v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68132">
        <property type="protein sequence ID" value="PNT68132"/>
        <property type="gene ID" value="BRADI_3g36465v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68133">
        <property type="protein sequence ID" value="PNT68133"/>
        <property type="gene ID" value="BRADI_3g36465v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D1K6"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D1K6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140750">
        <property type="term" value="F:nucleosome array spacer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="13"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT68133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="317" mass="37461" checksum="D33B85E066DDBCD6" modified="2018-03-28" version="1">MSYDKMIGFRDVRLKDLFYFGVTKDRTKMRKEFMCKTVGPDFPIILTTYWMAMTDEQRLTNCEWKYVVVDEGRELKKWEFELLDKLKGLPMDHKLLLIRSPFQNNLAELWSRLNFVMPRMFSSREEFDSWFDFPGKGGEEQQHEEKRALLSKLHAILRPFLRRQMEEDIENRVPQKKDIKNRVPQKKDIENRVPQKKVVTCYRNLFPSDNLLSSQYALEGNVVKRARTNAAPRPTSTLEECNNSFQAPSKPAVLVQVPNSTSSELIFKSLKEIPELARSDILRYYSALVRDDRQFESLMALPMDMRKDWLLMEIGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D1U1</accession>
    <name>A0A2K2D1U1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g37495v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D1U1"/>
    <dbReference type="EnsemblPlants" id="PNT68231">
        <property type="protein sequence ID" value="PNT68231"/>
        <property type="gene ID" value="BRADI_3g37495v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68231">
        <property type="protein sequence ID" value="PNT68231"/>
        <property type="gene ID" value="BRADI_3g37495v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D1U1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT68231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="60" mass="6454" checksum="785DCD9D9434BEFB" modified="2018-03-28" version="1">MPGKFVRVQTAPIIASLGLEMGPLLIMISFSAPKLGFASSWILATRVPKSGSWTKRYTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D9N4</accession>
    <name>A0A2K2D9N4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC104583133</name>
        <name evidence="12" type="ORF">BRADI_2g20931v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SWEET sugar transporter family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="BRADI_2g20931v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583133"/>
    <dbReference type="Gramene" id="PNT70985">
        <property type="protein sequence ID" value="PNT70985"/>
        <property type="gene ID" value="BRADI_2g20931v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF214">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-0677">Repeat</keyword>
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    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <end position="164"/>
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            <end position="198"/>
        </location>
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            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
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            <end position="300"/>
        </location>
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            <end position="256"/>
        </location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT70985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="300" mass="33058" checksum="CF6AF3797CAE6ABC" modified="2018-03-28" version="1">MEPAGAMEPAGQDTPAELTPAQTAVLILGYLSTFYVLLAPLKTIRAIKAAGAVGQETATMYLCVLCNSLVWLLYGVGADDKAIMGINGIGVLFQAYFLYVFIRASTDRFKQCSYVAMVLAASASVAIYVYVFPFIPDPKDRKKAFGQAGIFSGLAMYCGSWGRFRTVWRKGNVVRDLDLNVVVSSLLNGICWSIYAMLIDDDLLLTPNAVGIGLAEFQLGVYAWYYYMGSNDNNGDGGDDNINLLRDDDDEEEDDDHNNRRDDEEDDGDNNNRHDGGDDDNEEELLAPGRQATYRIQTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D9U7</accession>
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        <lineage>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </dbReference>
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    </dbReference>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="118" mass="13785" checksum="689FFADA9DD1D7E6" modified="2018-03-28" version="1">MAAINWNDDNTRIITELFADQVHRENRPNSHLNNVAYEEISQRFKDKTGIESKKTQIKNKWDKLKNEYDIWKKLLLEQTGAGWEGEPSTRTSNGGGRPKWTFLVVEGFKTKVFVMRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DB22</accession>
    <name>A0A2K2DB22_BRADI</name>
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            <fullName evidence="2 3">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="2" type="ORF">BRADI_2g28143v3</name>
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    <organism evidence="2">
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT71486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="PNT71486"/>
        <property type="gene ID" value="BRADI_2g28143v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT71486"/>
        <property type="gene ID" value="BRADI_2g28143v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DB22"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
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            <begin position="31"/>
            <end position="40"/>
        </location>
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            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="156"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="177" mass="18813" checksum="59940F046D113568" modified="2018-03-28" version="1" fragment="single">PWIWIGQTSGGLGFTGGGGARRRRRRRRRGTGGGEAGGSGKRASGAARPGEDGDGLGEARGERCACLGRRRGGGRPQNSSELRATAAVHENGDGARQKKRTKNDGGKTAAGGARVSGRNPAAWTHDSVAKDGGKREEAQGFKKTRSGSRIRDGARRSRRWRRRRRAGVTMRSRAVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DFM9</accession>
    <name>A0A2K2DFM9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g53182v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DFM9"/>
    <dbReference type="EnsemblPlants" id="PNT73093">
        <property type="protein sequence ID" value="PNT73093"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73094">
        <property type="protein sequence ID" value="PNT73094"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73095">
        <property type="protein sequence ID" value="PNT73095"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73096">
        <property type="protein sequence ID" value="PNT73096"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73097">
        <property type="protein sequence ID" value="PNT73097"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73098">
        <property type="protein sequence ID" value="PNT73098"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73093">
        <property type="protein sequence ID" value="PNT73093"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73094">
        <property type="protein sequence ID" value="PNT73094"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73095">
        <property type="protein sequence ID" value="PNT73095"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73096">
        <property type="protein sequence ID" value="PNT73096"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73097">
        <property type="protein sequence ID" value="PNT73097"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73098">
        <property type="protein sequence ID" value="PNT73098"/>
        <property type="gene ID" value="BRADI_2g53182v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DFM9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT73098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="361" mass="39483" checksum="48335F899EC36193" modified="2018-03-28" version="1">MVSTLRPSRKSMVNDRASPPTRAFSLAKPLYQSGGLVQRFGAGLVRRAGGDELRSEGWLVGDWEVWRSVAEIARFSTRLFALVETRNQRNLSGSLYLSSLKAEKSPALSTWPLGRRPFGIRGRFFAVCFRSPPEARDHPSLPAGEHSGPASPSPFPSLYLFGARVRACAAASLRVRHRHRVRTDQIDPDLLYALQYVLPKIDTRTYVIEDPASGGADLPFRADTHGEVINAPVPSPARERSKRRVEEENLWTDVSPIPSSARARGKRRMEENPSAEIPWYEASPSTPERPKSPTQSAAPLPPPVDGQSAIPSVGGSPAPISLPYYPLSPTAPLNPPLAIESQNPRAGLPEAGTQPQAHENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DMS3</accession>
    <name>A0A2K2DMS3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BUB1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g34982v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DMS3"/>
    <dbReference type="EnsemblPlants" id="PNT75570">
        <property type="protein sequence ID" value="PNT75570"/>
        <property type="gene ID" value="BRADI_1g34982v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75570">
        <property type="protein sequence ID" value="PNT75570"/>
        <property type="gene ID" value="BRADI_1g34982v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DMS3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT75570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="18720" checksum="629B2F5377AAA878" modified="2018-03-28" version="1">MGKAKPGPGETTQRRRMRAYESLDLDSAAALEPGSAQRLREAVRAMRAAEGREEARQLLRSAFSVPGAEDYAAVYKTWVAMEAAAGNVAAARELGTADEYAAFWIAYVAFELRHGGGAGSASARDAAVRATCAMAGVRLEEGGGAAGRPAPAPRCRWWTRLALLAVVRTAAAWVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DNV3</accession>
    <name>A0A2K2DNV3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g42365v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DNV3"/>
    <dbReference type="EnsemblPlants" id="PNT75959">
        <property type="protein sequence ID" value="PNT75959"/>
        <property type="gene ID" value="BRADI_1g42365v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75959">
        <property type="protein sequence ID" value="PNT75959"/>
        <property type="gene ID" value="BRADI_1g42365v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DNV3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT75959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="179" mass="19185" checksum="D580E84FE5B9D063" modified="2018-03-28" version="1">MALPSLNNNSTTEVAFTFLTFWSFSPSRAPALPNSRARPTSSLYPARVAAPPSHRHEGFRPCAEIPVAADRGEPVAGARAARRCRTRGESGRLYPSCTAPSTSFCCPSSGPVSGHGRRLASGHGGRLRCLRGCGDDVRWRGTHRRCAGSLASPRTAQAWAPATCSMICPNRHRSIKKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DPB7</accession>
    <name>A0A2K2DPB7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_1g44425v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DPB7"/>
    <dbReference type="STRING" id="15368.A0A2K2DPB7"/>
    <dbReference type="EnsemblPlants" id="PNT76124">
        <property type="protein sequence ID" value="PNT76124"/>
        <property type="gene ID" value="BRADI_1g44425v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76124">
        <property type="protein sequence ID" value="PNT76124"/>
        <property type="gene ID" value="BRADI_1g44425v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DPB7"/>
    <dbReference type="OrthoDB" id="1907415at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF30">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="26"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT76124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="526" mass="58572" checksum="07342CE066A1530F" modified="2018-03-28" version="1">MLPSLKVLLLVGCGLNNSPDFLLHSNLTSIETIDISWNHLQKSIAPNWFWDLTNLKQLDISYNKFSGPFPNEIGNMTSMKQLHLSNNNLVGMIPSNLKNLCNLEELHLSSNNINGSITEIFQRFPTCSLNKLAALALSDTDLAGSLPTNLQESFSNLTFLDLSENKLTGPVPLWIGELTKLTSLDLTNNNLDGVLHEGHLSGLPSLENLLLSGNSIAIIVNSTWFPPFSLTVIKLRSCILGPRFPLWLRWQKPLELDISNTGISDMVPDWFWIMVSLLDEINMPQNQLSGFLPSTMEHMRAYRIDLSSNQFSGPMPKLPVNLTYLDLSRNKLSGLPIEFGAPQLQVLILFGNSISGSIPSYFCELGSISVLDLSENKLTGPLPNCLHDESTTKKTTLSIRNLYLRNNNLSGDFPLLLQNCQELIFLDLAYNQFSGTLPSWIGEKLPSLAFLRLRSNMFHGHIPVGLTKLADLQYLDLSNNNISGGIPKSIVNFTGMIQRRDKYGKYDGTVILHKWRARGDLLAGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DT65</accession>
    <name>A0A2K2DT65_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g63273v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DT65"/>
    <dbReference type="EnsemblPlants" id="PNT77464">
        <property type="protein sequence ID" value="PNT77464"/>
        <property type="gene ID" value="BRADI_1g63273v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77464">
        <property type="protein sequence ID" value="PNT77464"/>
        <property type="gene ID" value="BRADI_1g63273v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DT65"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT77464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="143" mass="16001" checksum="63110DDFA2383E90" modified="2018-03-28" version="1">MIEFLQLADLLAAVVLFPGREDVLRWKWSASGVYSAQNRNTMAHLLMGCAFTKAVLHEVLARVGALCCLPRPSEDLVAWLSAGARRLQGKVKVTRSLLNLTLWRIWKLRNACIFEGATPVVARLVEDIFVEADLWRAAEPSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DV45</accession>
    <name>A0A2K2DV45_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g74434v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DV45"/>
    <dbReference type="STRING" id="15368.A0A2K2DV45"/>
    <dbReference type="EnsemblPlants" id="PNT78146">
        <property type="protein sequence ID" value="PNT78146"/>
        <property type="gene ID" value="BRADI_1g74434v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78146">
        <property type="protein sequence ID" value="PNT78146"/>
        <property type="gene ID" value="BRADI_1g74434v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DV45"/>
    <dbReference type="OrthoDB" id="360088at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT78146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="10321" checksum="735AFBE9EDA4577F" modified="2018-03-28" version="1">MPVRNVNYSIFSCGNGNEKHEILFLEIWTNGSLTPKEALYEASRNLIDLFLPFLHAEEEGTGFEENKNRFTPPLFTFQKRLTALTLRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0A341M6I1</accession>
    <name>A0A341M6I1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EnsemblPlants" id="PNT63746">
        <property type="protein sequence ID" value="PNT63746"/>
        <property type="gene ID" value="BRADI_4g20566v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63746">
        <property type="protein sequence ID" value="PNT63746"/>
        <property type="gene ID" value="BRADI_4g20566v3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="214" mass="23073" checksum="42EED0BD0D85AF83" modified="2025-10-08" version="1">MVLCSGPHVSASAFVSSSFLIPIASPDLKVSLASLFLSLSLLRFQQRFRPGYSPWSSGTARSVLSSAWSCVFGGGVWWRGLLAELAEGAKFCGEAGGRGSSCMSRGGVRGGYAEATAPLIHVLLPKSISASHLDHHGRIDFTGVAAFDHWWVESTTGFASREFPRSGHLCPSSFVPYFSGRFPLQFLIQATRCACTMMIHRSVASRRSNIAFCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPX1</accession>
    <name>I1GPX1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">NOT2/NOT3/NOT5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100823027</name>
        <name evidence="14" type="ORF">BRADI_1g13450v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231942.1">
        <property type="nucleotide sequence ID" value="XM_010233640.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPX1"/>
    <dbReference type="FunCoup" id="I1GPX1">
        <property type="interactions" value="2323"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GPX1"/>
    <dbReference type="EnsemblPlants" id="KQK13929">
        <property type="protein sequence ID" value="KQK13929"/>
        <property type="gene ID" value="BRADI_1g13450v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823027"/>
    <dbReference type="Gramene" id="KQK13929">
        <property type="protein sequence ID" value="KQK13929"/>
        <property type="gene ID" value="BRADI_1g13450v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2150">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013819_3_0_1"/>
    <dbReference type="OMA" id="YKPQTPY"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GPX1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1020:FF:000003">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012270">
        <property type="entry name" value="CCR4-NOT_su3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005290">
        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="12">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="13">
        <location>
            <begin position="722"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="265"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="470"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="579"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="41"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="486"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="628"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="645"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK13929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="866" mass="95344" checksum="7B727452F07E963A" modified="2012-06-13" version="1">MGASRKLQGEIDRVLKKVQEGVDVFDSIWNKVYDTENANQKEKFEADLKKEIKKLQRYRDQIKTWIQSSEIKDKKVSASYEQALMDARKQIEREMERFKVCEKETKTKAFSKEGLGQQPKTDPREKAKAETRDWLNSVVSDLENQIDNFEAELEGLSFKKGKQRPPRLVHLEKSITRHKAHIKKLESILRLLDNDELSPEQVNDVKDFLEDYVERNQEDFDEFSDVEDLYSTLPMEKVEALEDMVSLAPSSLIKGVAAVSTTAVLSTKSPVATSPTQATISTTSQSTSQDQAEETASLESNPELVPQTPPPKGGNLGPSVPVVPTVISTSIAAVSVPADTISSPGPVRLTVPPTVPAIFSASATVRSAPESIPAVTSIPANASSALKDEDSMNFPPRRSSPAITEIGLGRGITRGLTSQTLGAAPISVVPVPVPGNGSISAVPAINDLSKRNTLSTDERVNSVGLSQQLISPLGNKVQPQPVPRTNDATNSDSNNQSESAMLGGRVFSPPVVSGVQWRPQAPAGFQNQSETGQFRGRPELADQREKYLQRLQQVQQQQGNLLNVSNITGINQKQFSTQQPNSLLQQFNSQNSSSSSQAGLGLGGQGQDNVHTKSEEQQGMTEDISVESAATTGANKTTNEDDTKTPYSNPSASIAEGTQLSRDSDLSPGQPLQPGMPSSGVGVIGRRSVSDFGAIGDNLSGTSAASGHDQLYNLQMLEAAYHRLPQPKDSERAKNYIPRHPSVTPASYPQIQAPIVTNPAFWERIGSDALATDMLFFAFYYQQNSYQQYLAARELKKQSWRFHRKYNTWFQRHVEPQVTTDDYERGSYVYFDFHLSDDGNGWCQRIKNDFTFEYNFLEDELSVQPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSB9</accession>
    <name>I1GSB9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100826006</name>
        <name evidence="3" type="ORF">BRADI_1g21380v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562690.1">
        <property type="nucleotide sequence ID" value="XM_003562642.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSB9"/>
    <dbReference type="STRING" id="15368.I1GSB9"/>
    <dbReference type="EnsemblPlants" id="KQK15233">
        <property type="protein sequence ID" value="KQK15233"/>
        <property type="gene ID" value="BRADI_1g21380v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826006"/>
    <dbReference type="Gramene" id="KQK15233">
        <property type="protein sequence ID" value="KQK15233"/>
        <property type="gene ID" value="BRADI_1g21380v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826006"/>
    <dbReference type="eggNOG" id="KOG0207">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080283_1_0_1"/>
    <dbReference type="OMA" id="PARCFAG"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000047">
        <property type="entry name" value="Copper-transporting ATPase PAA1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="76"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK15233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="157" mass="16163" checksum="FEA9462FDCB56CE7" modified="2012-06-13" version="1">MESTLTTRLPLLPSPSKSRALAYPPLAGARRVASISFSPGSVHGFLLSRGDGDAGVTSSITAAAAAIGDAVDPASEAILLSVQGMMCDGCAASVKRILEGQPEVTSATVDFKEAKAVVWTTPEVKLAEDWQKQCGEKLASHLGTCGFESQAQGQGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTK5</accession>
    <name>I1GTK5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100844026</name>
        <name evidence="4" type="ORF">BRADI_1g25050v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560104.1">
        <property type="nucleotide sequence ID" value="XM_003560056.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GTK5"/>
    <dbReference type="FunCoup" id="I1GTK5">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GTK5"/>
    <dbReference type="EnsemblPlants" id="KQK15818">
        <property type="protein sequence ID" value="KQK15818"/>
        <property type="gene ID" value="BRADI_1g25050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844026"/>
    <dbReference type="Gramene" id="KQK15818">
        <property type="protein sequence ID" value="KQK15818"/>
        <property type="gene ID" value="BRADI_1g25050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844026"/>
    <dbReference type="eggNOG" id="KOG0108">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109125_0_0_1"/>
    <dbReference type="OMA" id="DETRCRN"/>
    <dbReference type="OrthoDB" id="272703at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001304">
        <property type="entry name" value="Os06g0248200 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF4">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="17"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK15818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="230" mass="24722" checksum="5D14EC2CC28BCCFF" modified="2012-06-13" version="1">MDGLAVAAAAANCRCSRVVYVGNIPFHATEKEVRDACELIGPVLSFRLAADAATGKRKGYAFVEYADDATARSACRNLHGHPLRGRDLRVGLADRASIRRRRHGDHDPIGMDDAIHAASLVDSAARPAVIASMARHLAGLSRHQLREAAAEFEKHGPDTCEILKKHIPGLDTAMEMVQRLLEMAAADDAAEEAKRKKRASGCLNAEESDQHAKLRKMEDGGKVPAGVACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1GVN9</accession>
    <name>I1GVN9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein GLUTAMINE DUMPER 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100821871</name>
        <name evidence="10" type="ORF">BRADI_1g31210v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLUTAMINE DUMPER 1 (TC 9.B.60) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560379.1">
        <property type="nucleotide sequence ID" value="XM_003560331.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GVN9"/>
    <dbReference type="STRING" id="15368.I1GVN9"/>
    <dbReference type="EnsemblPlants" id="KQK16881">
        <property type="protein sequence ID" value="KQK16881"/>
        <property type="gene ID" value="BRADI_1g31210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821871"/>
    <dbReference type="Gramene" id="KQK16881">
        <property type="protein sequence ID" value="KQK16881"/>
        <property type="gene ID" value="BRADI_1g31210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821871"/>
    <dbReference type="eggNOG" id="ENOG502S4AK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112624_0_0_1"/>
    <dbReference type="OMA" id="CEQGVPR"/>
    <dbReference type="OrthoDB" id="1930784at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080143">
        <property type="term" value="P:regulation of amino acid export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040359">
        <property type="entry name" value="GDU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33228:SF82">
        <property type="entry name" value="OS06G0654400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33228">
        <property type="entry name" value="PROTEIN GLUTAMINE DUMPER 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="141"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK16881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="18247" checksum="21650D4C5F5AA811" modified="2012-06-13" version="1">MRPGAEFAMVHGGAMMAPTAAPGGHALTNGTHSPWQSPVPYLFGGLAAMLGLIAFALLILACSYWKLSGYLDGGAHGSGAGGGDDDGEKGSVGGAARPAAAVQEHVVVIMAGEERPTFLAMPSASRAAAVELGAMANPEASASALGGGDGAPELEKKIEDSPQLVGGGSGSESSGATSTALRESSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1GW57</accession>
    <name>I1GW57_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100840500</name>
        <name evidence="6" type="ORF">BRADI_1g32660v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563412.1">
        <property type="nucleotide sequence ID" value="XM_003563364.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GW57"/>
    <dbReference type="FunCoup" id="I1GW57">
        <property type="interactions" value="882"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GW57"/>
    <dbReference type="EnsemblPlants" id="KQK17141">
        <property type="protein sequence ID" value="KQK17141"/>
        <property type="gene ID" value="BRADI_1g32660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840500"/>
    <dbReference type="Gramene" id="KQK17141">
        <property type="protein sequence ID" value="KQK17141"/>
        <property type="gene ID" value="BRADI_1g32660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840500"/>
    <dbReference type="eggNOG" id="ENOG502QRZB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035664_5_1_1"/>
    <dbReference type="OMA" id="WDIPEIA"/>
    <dbReference type="OrthoDB" id="744205at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF96">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 21_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="5">
        <location>
            <begin position="11"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK17141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="303" mass="33758" checksum="298AB284F2F38D17" modified="2012-06-13" version="1">MNSLSMVEARLPPGFRFHPRDDELVLDYLARKLVGGGGAAAPTIYGCPTMVDVDLNKVEPWDLPEIACIGGKEWYFYSLRDRKYATGQRTNRATESGYWKATGKDRPISRKGLLVGMRKTLVFYQGRAPKGKKTEWVMHEFRKEGQGDPMKLPFKEDWVLCRVFYKSRATIAKPPTGNNYNIGSAAATSLPPLIDNYIAFDHSGMSVQNLEGYEQVPCFSNNPSHPLSSTSMSMNIPVTAGAPTADQEQQHVGKAIKDVLSQFTRFDQGNVKREAPPQGGLFAQDGFEYLAESGFSQMWNSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>I1GY15</accession>
    <name>I1GY15_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC100844548</name>
        <name evidence="21" type="ORF">BRADI_1g37960v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">Bd21</strain>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563758.1">
        <property type="nucleotide sequence ID" value="XM_003563710.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GY15"/>
    <dbReference type="EnsemblPlants" id="KQK18000">
        <property type="protein sequence ID" value="KQK18000"/>
        <property type="gene ID" value="BRADI_1g37960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844548"/>
    <dbReference type="Gramene" id="KQK18000">
        <property type="protein sequence ID" value="KQK18000"/>
        <property type="gene ID" value="BRADI_1g37960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844548"/>
    <dbReference type="eggNOG" id="ENOG502QQPF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_22_1_1"/>
    <dbReference type="OMA" id="VKRIWNT"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000714">
        <property type="entry name" value="Kinase family with leucine-rich repeat domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000642">
        <property type="entry name" value="Leucine-rich receptor-like protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000221">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000228">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000512">
        <property type="entry name" value="Receptor-like protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF29">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN KINASE HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094183" description="Protein kinase domain-containing protein" evidence="19">
        <location>
            <begin position="22"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="630"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="684"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="995"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="722"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQK18000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1028" mass="111758" checksum="25117DA942D3899F" modified="2012-06-13" version="1">MSNTYPFFPLLLLLLAGESLSQPTTGDQASLLAIKNAWGNPSQLASWDPAAHADHCRNWTGVACQGAVVTGLTLPSLNLTGKVPESLCDLASLARLDLSSNKLSGAFPGAALYGCSKLRFLDLSYNAFDGALPDDINLILSPAMEHLNLSNNHFSGVLPPAVARLPLLKSLLLDTNQFTGSYPAREISELKGLQQLTLALNAFEPAPAPVEFAQLTNLTYLWMSNMNVTGEIPEAYSSLTELTVLGLSTNNLTGEIPAWVWRHPKLQLVYLFTNGLNGELPRSIAAANWVEFDVSTNRLTGQISEDFGNHKNLTLLFLYKNQLTGTIPASIATLPNLKDIRLFENKLSGELPKELGKHSPLGNLEVCNNNLSGPLPASLCANGKLYDIVVFNNDFSGQLPAGLGDCVLLNNLMMYNNRFSGEFPAKMWSFPMLTTLMIQNNGFTGALPAQISENLTRIEMGNNKFSGSFPTSATGLHVFKAENNLLSGELPANMSGFANLSDLLIAGNRLSGSIPTSVSLLQKLNSLNMSGNRISGAIPPSSIGLLPSLTLLDLSHNELTGAIPSDFSNLNFNLLNMSSNQLIGEVPLSLQIAAYEHSFLGNVGLCTKHDSGIGLPACGSIARDELSKGLIILFAMLAAIVLIGSVGIAWLLFRRRKDSQDVTDWKMTQFTHVGFTESDVLNNIREENVIGSGGSGKVYRIHLPARGRDEEHGGGGMVAVKKIWNAKKMDAKHDKEFESEVKVLGNIRHNNIVKLLCCISSTDAKLLVYEYMENGSLDRWLHHREREGAPAPLDWPTRLAIAIDSAKGLSYMHHDCAQSIVHRDIKTSNILLDPEFHAKIADFGLARMLVKFGEPESVSAIGGTFGYMAPEYGHRPRMNEKVDVYSFGVVLLELTTGKVANDSGADFCLAEWAWRRYQKGPPLNDAIDEHIRDPAYLPDILAVFTLGVICTGENPSTRPSMKEVLQHLTRCDRMSNAEAQACQLDYVNGGGTPLLEAKKGSRRRSMSSSGRWGDDDGEDSGNFVVHAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYX9</accession>
    <name>I1GYX9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane protein 245</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100823966</name>
        <name evidence="8" type="ORF">BRADI_1g43240v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563938.1">
        <property type="nucleotide sequence ID" value="XM_003563890.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYX9"/>
    <dbReference type="EnsemblPlants" id="KQK18550">
        <property type="protein sequence ID" value="KQK18550"/>
        <property type="gene ID" value="BRADI_1g43240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823966"/>
    <dbReference type="Gramene" id="KQK18550">
        <property type="protein sequence ID" value="KQK18550"/>
        <property type="gene ID" value="BRADI_1g43240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823966"/>
    <dbReference type="eggNOG" id="KOG2365">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028708_1_0_1"/>
    <dbReference type="OMA" id="WLVSFWI"/>
    <dbReference type="OrthoDB" id="5970161at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF72">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN C9ORF5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="495"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="559"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="611"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK18550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="642" mass="69822" checksum="96861B8595ECD233" modified="2012-06-13" version="1">MALVPSGDPAAVCNTTIPWSKMFRDASVRRPKQPEDPPPKPTAPGKKAAASPDIAGLSLEPDARLALYIAMAHAGLATALLVLYGLYMLLADFLRPMQWALLCSIPLRETQHALVAFWEPPLRGGFSATVLALPLAALRSCAATLADARAALLRRPLPPSPSFPRLLRWLASSLFFLLLFERLGSAAALLLLALSLAFFAASPKPSSFLSRAATSRISSRNPSSRGLLLTGGILRHLKTLVAVGLMLGMIAGFLAGSVFFSYKIGLEGKDAVMSLKSHVENGNYSEKIGLKNWLDDNDIPGLVDQYSAKLYDTVWEQLDQLAVQYNLTDFTSGFRHFLISQSVGPSGAKSKELITSGPHPYSMKLQSIAVRVRKREWVEIYKELDSFFRELLITREDLVVKAKDLALQGTEIAKSLLSSSTSVLGGSANLMLSIALRILSGAAEVLNFVSQLMVFLWVLYYLITVEGGGATEQVIDLLPVSKQVKDRCVEVIDHAISSVLLATAKIAIFQGCLTWLLLKFFKVHFVYTSTVLTIISALFPILPPWLSSIFAAGQLLTEGRYVLTVMVTVIHLVIMDYGTTVIQEDIRGYNGYLTGLSIIGGMTLFPNALEGAILGPLIMTVVIALKNLYTEFVLADTEETSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1H108</accession>
    <name>I1H108_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquinone biosynthesis O-methyltransferase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinol 3-O-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.64</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinone 3-O-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Polyprenyldihydroxybenzoate methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.114</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100838272</name>
        <name evidence="5" type="synonym">COQ3</name>
        <name evidence="7" type="ORF">BRADI_1g49240v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">O-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis. Catalyzes the 2 O-methylation of 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid into 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid. Also catalyzes the last step of ubiquinone biosynthesis by mediating methylation of 3-demethylubiquinone into ubiquinone. Also able to mediate the methylation of 3-demethylubiquinol into ubiquinol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-methionine = a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="2.1.1.114"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinone + S-adenosyl-L-methionine = a ubiquinone + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:81215"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.64" evidence="5"/>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EC" id="2.1.1.114" evidence="5"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564324.1">
        <property type="nucleotide sequence ID" value="XM_003564276.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H108"/>
    <dbReference type="FunCoup" id="I1H108">
        <property type="interactions" value="1871"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H108"/>
    <dbReference type="EnsemblPlants" id="KQK19599">
        <property type="protein sequence ID" value="KQK19599"/>
        <property type="gene ID" value="BRADI_1g49240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838272"/>
    <dbReference type="Gramene" id="KQK19599">
        <property type="protein sequence ID" value="KQK19599"/>
        <property type="gene ID" value="BRADI_1g49240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838272"/>
    <dbReference type="eggNOG" id="KOG1270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042432_2_0_1"/>
    <dbReference type="OMA" id="LASRWWD"/>
    <dbReference type="OrthoDB" id="3265906at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061542">
        <property type="term" value="F:3-demethylubiquinol 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010420">
        <property type="term" value="F:polyprenyldihydroxybenzoate methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK19599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="329" mass="35033" checksum="FA78C95AD85D1BC0" modified="2012-06-13" version="1">MLRRLPSSLRRALASSAIPPRGARVANPTLSSPHALTPQWRGCASASAAGSSSPSSLPPPPPSPPQGPPPHGRGSAASSLNSAEVAKFAAIAETWWDSQGPFKPLHLMNPTRLSFIRSTLCRHFRRDPYSAKPLEGLKVIDVGCGGGILSEPLARMGAAVTGIDAVDKNIKIAQIHAASDPTTASIEYCCTTAEDLVKESNQFDAVISLEVIEHVANPLEFCKSLSALTVPNGATVISTINRSMRAYATAIVAAEYILRWLPKGTHEWSKLVTPEELALALQRASISVQEMAGFVYNPLTGGWSLSDDISINYIAYGIKKSETPSTMPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2K9</accession>
    <name>I1H2K9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100826063</name>
        <name evidence="3" type="ORF">BRADI_1g53857v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228219.1">
        <property type="nucleotide sequence ID" value="XM_010229917.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2K9"/>
    <dbReference type="STRING" id="15368.I1H2K9"/>
    <dbReference type="EnsemblPlants" id="KQK20328">
        <property type="protein sequence ID" value="KQK20328"/>
        <property type="gene ID" value="BRADI_1g53857v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826063"/>
    <dbReference type="Gramene" id="KQK20328">
        <property type="protein sequence ID" value="KQK20328"/>
        <property type="gene ID" value="BRADI_1g53857v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826063"/>
    <dbReference type="eggNOG" id="ENOG502QRYT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074465_0_0_1"/>
    <dbReference type="OMA" id="FVWIQMY"/>
    <dbReference type="OrthoDB" id="1916950at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H2K9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35471">
        <property type="entry name" value="OS07G0223700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35471:SF1">
        <property type="entry name" value="OS07G0223700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK20328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="252" mass="27818" checksum="853F92F1033AF970" modified="2012-06-13" version="1">MPWCPGFGPRVRSFLRDYDALQSLALALIYLQIGCALIGSLGALFNGVLVINLVIGLFAVVAIESSSQRLGRTYAVLLFFAIVLDVAWFILFSHAIWNTTPDENYGELFVFSLRLALWMQIIGFSVRFLSSFVWIQMYRLGVSSSTPTYYEANDARNSFLSPRSNSVRRSSMADDILGGSIYDPSYYSSLFEDVRNSACNHQGDKQSGSNDSGSTSAGQSPRLKTFGTRSFLSNDVEAGLRKPLNSCTVETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H8K7</accession>
    <name>I1H8K7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100834742</name>
        <name evidence="12" type="ORF">BRADI_1g71370v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558585.1">
        <property type="nucleotide sequence ID" value="XM_003558537.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H8K7"/>
    <dbReference type="FunCoup" id="I1H8K7">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK23114">
        <property type="protein sequence ID" value="KQK23114"/>
        <property type="gene ID" value="BRADI_1g71370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834742"/>
    <dbReference type="Gramene" id="KQK23114">
        <property type="protein sequence ID" value="KQK23114"/>
        <property type="gene ID" value="BRADI_1g71370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834742"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="OMA" id="CGIQAFT"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000025">
        <property type="entry name" value="probable polyol transporter 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF49">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="392"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="32"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003346"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK23114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="526" mass="56498" checksum="58B46292A02BE72C" modified="2012-06-13" version="1">MMSTGTDTTADAVPVAVAPAKRPPINKYAFACALLASMNSVLLGYDISVMSGAQLFMKEDLKITDTQIEILAGVINIYSLFGSLAAGLTSDWLGRRYTMVLAAAIFFTGALLMGLAPNYAFLMAGRFVAGIGVGYALMIAPVYTAEVAPTSARGFLTSFPEVFNNFGILLGYVSNFAFARLPVHLSWRAMFLVGAVPPIFLGFAVLAMPESPRWLVMRGRIDDARYVLQRTSDSPEEAEERLLDIKRVVGIPADATDADDVVAIVRANEAARGQGVWKELLINPSRPVRRMLVAGLGLMFIQQATGVDCVVMYSPRVFEQAGIKSKTNSLGASMAVGVCKTFFIPISTLLLDRVGRRPLLLASGGGMAIFLFTLATSLLMMDRRPESESKALGAVSIAAMLSFVASFASGLGPVAWVYCSEIYPLRLRAQAAAIGTGLNRLMSGATTMSFLSLSHTITIAGSFYLYACVATAGWVFMYFFLPETMGKSLEDTGKLFGKDTDDDVDGGAVVAVRHERKKSTELVAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAE3</accession>
    <name>I1HAE3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Peroxidase</fullName>
            <ecNumber evidence="17">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100843601</name>
        <name evidence="19" type="ORF">BRADI_1g77140v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 17">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="14 17">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 17">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="14 17">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="17"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558949.1">
        <property type="nucleotide sequence ID" value="XM_003558901.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAE3"/>
    <dbReference type="FunCoup" id="I1HAE3">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HAE3"/>
    <dbReference type="EnsemblPlants" id="KQK23931">
        <property type="protein sequence ID" value="KQK23931"/>
        <property type="gene ID" value="BRADI_1g77140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843601"/>
    <dbReference type="Gramene" id="KQK23931">
        <property type="protein sequence ID" value="KQK23931"/>
        <property type="gene ID" value="BRADI_1g77140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843601"/>
    <dbReference type="eggNOG" id="ENOG502QPX7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010543_0_1_1"/>
    <dbReference type="OMA" id="MLCPAAE"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF84">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 17" id="KW-0349">Heme</keyword>
    <keyword evidence="11 17" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="7 14 17" id="KW-0408">Iron</keyword>
    <keyword evidence="4 14 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 17" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="10" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0964">Secreted</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982731" description="Peroxidase" evidence="17">
        <location>
            <begin position="35"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="18">
        <location>
            <begin position="35"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="15">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="45"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="78"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="130"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQK23931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="333" mass="34699" checksum="1A813098EF93F350" modified="2012-06-13" version="1">MEAQARGGLRIPIPIRMRLLVVMLVLMAARPAMAQLAVGYYDTLCPAAEIIVQEEVSKGVSGSPGTAAGLLRLHFHDCFVRGCDASVLLDSTPGNKAEKDAPPNSSLRGFDVIDKAKTRLEQACYRVVSCADILAFAARDALALVGGSAYQVPAGRRDGNVSSAGETNGNLPPPTANVNQLTQIFGSKGLSKAQMVTLSGAHTVGAAQCSSFSSRLYSSGPNGGQDPTMDPKYLTALTAQCPQKGAQQAVPMDPVTPNAFDTNYYANLVANRGLLSSDQALLADPNASAQVVAYTSSPDTFQTDFANAMIAMGNVGVLTGNAGNIRTNCRVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1HEZ4</accession>
    <name>I1HEZ4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100840113</name>
        <name evidence="14" type="ORF">BRADI_2g11990v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the splicing factor SR family. SR subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567614.1">
        <property type="nucleotide sequence ID" value="XM_003567566.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HEZ4"/>
    <dbReference type="STRING" id="15368.I1HEZ4"/>
    <dbReference type="EnsemblPlants" id="KQK04151">
        <property type="protein sequence ID" value="KQK04151"/>
        <property type="gene ID" value="BRADI_2g11990v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840113"/>
    <dbReference type="Gramene" id="KQK04151">
        <property type="protein sequence ID" value="KQK04151"/>
        <property type="gene ID" value="BRADI_2g11990v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840113"/>
    <dbReference type="eggNOG" id="KOG0105">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="FRSHEHT"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HEZ4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12599">
        <property type="entry name" value="RRM1_SF2_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000410">
        <property type="entry name" value="ASF/SF2-like pre-mRNA splicing factor SRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000062">
        <property type="entry name" value="serine/arginine-rich splicing factor SR34A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF62">
        <property type="entry name" value="SERINE_ARGININE (SR)-TYPE SHUTTLING MRNA BINDING PROTEIN NPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="7"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="110"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="192"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="228"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK04151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="262" mass="29917" checksum="0DBE0115077CE894" modified="2012-06-13" version="1">MGRTNSCTIYVGNLPGDIREREVEDLFYKYGRIVDIDLKIPPRPPGYAFVEFEDPRDADDAIYGRDGYNFDGYRLRVELAHGGRGQSYQYERPRSYSSGRRGGVSRRSEYRVLVDGLPSSASWQDLKDHMRRAGDVCFSDVYREAGAIVGIVDYTNYDDMKYAIRKLDGTEFRNAFSRSYIRVREYDARSRSRSRSLSYSRSRSCSRSESPRSVSRSPLPVDERSLSRSRSPDSSASPRKSVSRSPSRSRSLSRSASPVRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HFN9</accession>
    <name>I1HFN9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100835633</name>
        <name evidence="13" type="ORF">BRADI_2g14120v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase HD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567768.1">
        <property type="nucleotide sequence ID" value="XM_003567720.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HFN9"/>
    <dbReference type="FunCoup" id="I1HFN9">
        <property type="interactions" value="1707"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HFN9"/>
    <dbReference type="EnsemblPlants" id="KQK04537">
        <property type="protein sequence ID" value="KQK04537"/>
        <property type="gene ID" value="BRADI_2g14120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835633"/>
    <dbReference type="Gramene" id="KQK04537">
        <property type="protein sequence ID" value="KQK04537"/>
        <property type="gene ID" value="BRADI_2g14120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835633"/>
    <dbReference type="eggNOG" id="ENOG502QVH6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061903_0_0_1"/>
    <dbReference type="OMA" id="GKNEVHI"/>
    <dbReference type="OrthoDB" id="2019803at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HFN9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.340:FF:000004">
        <property type="entry name" value="Histone deacetylase HDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="97"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="160"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK04537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="296" mass="31739" checksum="AB4DAECB853CD23D" modified="2012-06-13" version="1">MEFWGLELKPGQTVKVEPEPEHFLHLSQGALGESKKDDKATMFVKIDGKKLAIGTLSTDKFPQIQFDLVFEKDFELSHSSKSSSVFFSGYKVFQPAEGDEMDFDSEGSEESEEDEEEAVIPVTKENGKAEGKQKQVKIDAGSSKAKPVLKAVGNKKGGDESSDDDESDESDDDIADLSDDDSGSEDGTSEESDEEEEEETPKKSTGKKRAAETVLKTPASDKKAKAATPSGQKTGDKKGAVHVATPHPAKKASKTPATSDKSPKSGGSVACKSCSKTFNSETALQSHSKAKHNEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HFQ2</accession>
    <name>I1HFQ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100837783</name>
        <name evidence="5" type="ORF">BRADI_2g14220v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567775.1">
        <property type="nucleotide sequence ID" value="XM_003567727.4"/>
    </dbReference>
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    <dbReference type="FunCoup" id="I1HFQ2">
        <property type="interactions" value="1222"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK04553">
        <property type="protein sequence ID" value="KQK04553"/>
        <property type="gene ID" value="BRADI_2g14220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837783"/>
    <dbReference type="Gramene" id="KQK04553">
        <property type="protein sequence ID" value="KQK04553"/>
        <property type="gene ID" value="BRADI_2g14220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837783"/>
    <dbReference type="eggNOG" id="ENOG502QRVZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="WINGPAR"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HFQ2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="CCT_domain"/>
    </dbReference>
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        <property type="entry name" value="CONSTANS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319:SF71">
        <property type="entry name" value="CCT MOTIF FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51017">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="275"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK04553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="327" mass="34716" checksum="328A8B54BDCE0CA5" modified="2012-06-13" version="1">MYADAGFGGFSVYSPPAPGSSCFFSTHPDLSYSCFSSSPAPAPAPAPAAAAAAAAPPLLLPADAPLDAAIFDNLGDMGQPSLISEYDLGAEGDLFKAPEPIIEEPLLSLDPVAAAISMMSGGAAENAMDETIKAAAADMGLSEVLYECEKELMEKSAIEETISELLDVKIPMLQVEQEASAAAGECSLHKSVSSGCLNSADWMNGTAAAANATVRPNFLDFQGLDFEAAFGMRRAFSEGDIQNLGANTPRTGNSGNVQASCERLVTIADLKTEERKQKLSRYRKKKVKRNFGRKIKYACRKALADSQPRVRGRFAKMDDGDMLKPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJP1</accession>
    <name>I1HJP1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g26115v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJP1"/>
    <dbReference type="FunCoup" id="I1HJP1">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HJP1"/>
    <dbReference type="EnsemblPlants" id="KQK06385">
        <property type="protein sequence ID" value="KQK06385"/>
        <property type="gene ID" value="BRADI_2g26115v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06385">
        <property type="protein sequence ID" value="KQK06385"/>
        <property type="gene ID" value="BRADI_2g26115v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138190_0_0_1"/>
    <dbReference type="InParanoid" id="I1HJP1"/>
    <dbReference type="OMA" id="SGIMLIH"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44579:SF4">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44579">
        <property type="entry name" value="OS01G0730500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="55"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK06385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="119" mass="12862" checksum="F6119640F258F7CA" modified="2012-06-13" version="1">MATPVASSSFPPHGRAVPFGRRAPLPSPRRSRALPVSALGSGEGNERRILDPLATPFQILGLNASAAYSASQLKAAFRARVKEFHPDVCKDKESANLIMRRVLEAYEMLSGNNGMMVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1HKQ6</accession>
    <name>I1HKQ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BRADI_2g31600v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class IV subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HKQ6"/>
    <dbReference type="EnsemblPlants" id="KQK06940">
        <property type="protein sequence ID" value="KQK06940"/>
        <property type="gene ID" value="BRADI_2g31600v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06940">
        <property type="protein sequence ID" value="KQK06940"/>
        <property type="gene ID" value="BRADI_2g31600v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUAY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="I1HKQ6"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
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        <property type="entry name" value="HD-Zip_IV"/>
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    <dbReference type="InterPro" id="IPR017970">
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        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN MERISTEM L1"/>
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    </dbReference>
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        <property type="entry name" value="PDF2_C"/>
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        <property type="entry name" value="START"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="62"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="14">
        <location>
            <begin position="250"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="64"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="116"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK06940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="772" mass="83400" checksum="99501C12905C16CB" modified="2012-06-13" version="1">MDGEGQQGSSDHNIELDFSDDNNLGDETDGVSYEPNTDDDANAAAANEDSNAGGGGAAAAGRTAPRSSKRHRREQIQQLEAVFQQCPHPDEQLRLDLSKRLGMGLLQVKFWFQNRRSAKKNKMEQQEGKKLREENEMLLAENKAMKAEIQSRTCIGCGGPRMHIHDCRDTPEKQRLRMENAMLKDQLMRTKVFVSVLTGKDVDDAAAAAEEGALPAAYSPYGLNNGGRPLVINPAAAVPAPSMSSAARSIAASQITLLDHLIGACEEFKMIASMNEPMWLRTSDGDVLNNQAYKNATYPGILGICPKGFAVDGTRTTGIVLGNAADLTSIFMDPARWSEMFPGIIVAGVTSSAIINSSASQPRLVQLVQMSAELKVLAPRAPVVRVRFMRQCMRLQPRLWAVVDVSVDGFIGHNDEKTLDLYQTAAALPTMYTAYRLLPSGCIIEEMDDGHCKVTCIVHAEYDESNIQPLFHPLLRSGQAFGARRWLASLQRQSEYFAVLRSDLVLNLNNTGAAMKPAGKKGILELAQRMTMNFYVAMCGLPAGQPWNDWNGGIGVGSERFELAVHFVTLDHQGVLSATTTVWLPGVPAGRVFHYLCDGSRRGEWDSLASSGPMKEVACVATGQLYGNSVSVLRPSVYNGTTNKNDKLILQEAYADASCMFMAYAPIDKQSMHQVMNGGGHTSFSLSPCGFIVLPDGQGQQSVQTISAGSSSSAGVSATCAGSIVSALYQTELSAPLQQSVAIGLMDDIGNLFCHAIKKIKDAVQAKLVVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1HL93</accession>
    <name>I1HL93_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100840918</name>
        <name evidence="9" type="ORF">BRADI_2g34000v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DVL/RTFL small polypeptides family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568811.1">
        <property type="nucleotide sequence ID" value="XM_003568763.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HL93"/>
    <dbReference type="STRING" id="15368.I1HL93"/>
    <dbReference type="EnsemblPlants" id="KQK07219">
        <property type="protein sequence ID" value="KQK07219"/>
        <property type="gene ID" value="BRADI_2g34000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840918"/>
    <dbReference type="Gramene" id="KQK07219">
        <property type="protein sequence ID" value="KQK07219"/>
        <property type="gene ID" value="BRADI_2g34000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840918"/>
    <dbReference type="eggNOG" id="ENOG502SCQN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150897_2_0_1"/>
    <dbReference type="OMA" id="LRRRCYK"/>
    <dbReference type="OrthoDB" id="1693826at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012552">
        <property type="entry name" value="DVL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051525">
        <property type="entry name" value="DVL_RTFL_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33102">
        <property type="entry name" value="DVL19-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08137">
        <property type="entry name" value="DVL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK07219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="80" mass="8921" checksum="CE2FA82CEBD51A18" modified="2012-06-13" version="1">MELAASASASVCSSYHHLSTQADADDGGAPSTPRRRKHAAAGCGLRRRCYTVLKQQRTRLYILRRCVSMLLCWNEHDLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I1HN91</accession>
    <name>I1HN91_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100832132</name>
        <name evidence="1" type="ORF">BRADI_2g40010v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566730.1">
        <property type="nucleotide sequence ID" value="XM_003566682.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315411.1">
        <property type="nucleotide sequence ID" value="XM_024459643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HN91"/>
    <dbReference type="STRING" id="15368.I1HN91"/>
    <dbReference type="EnsemblPlants" id="KQK08156">
        <property type="protein sequence ID" value="KQK08156"/>
        <property type="gene ID" value="BRADI_2g40010v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832132"/>
    <dbReference type="Gramene" id="KQK08156">
        <property type="protein sequence ID" value="KQK08156"/>
        <property type="gene ID" value="BRADI_2g40010v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832132"/>
    <dbReference type="eggNOG" id="ENOG502S8S6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091877_0_0_1"/>
    <dbReference type="OMA" id="MEVICPE"/>
    <dbReference type="OrthoDB" id="1917254at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33384">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33384:SF27">
        <property type="entry name" value="OS05G0102500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK08156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="161" mass="17845" checksum="F46012381BF076A1" modified="2012-06-13" version="1">MEHCTMRMPDRRSRFWQMDAQPVRIEVICPQPRRASRSPFLMENRANSKPNGTLPMYRADSSSEIYDLILSKNNPDVDTDSTRFFCGSPPVRTNNPLVRDPKFGTELPSLSPVGSPISKMLEMLGRIEVGTSPTRGASSNSISPKVRVEGFACGGNKDAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPG0</accession>
    <name>I1HPG0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100829496</name>
        <name evidence="2" type="ORF">BRADI_2g43670v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569366.1">
        <property type="nucleotide sequence ID" value="XM_003569318.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPG0"/>
    <dbReference type="STRING" id="15368.I1HPG0"/>
    <dbReference type="EnsemblPlants" id="KQK08747">
        <property type="protein sequence ID" value="KQK08747"/>
        <property type="gene ID" value="BRADI_2g43670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829496"/>
    <dbReference type="Gramene" id="KQK08747">
        <property type="protein sequence ID" value="KQK08747"/>
        <property type="gene ID" value="BRADI_2g43670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829496"/>
    <dbReference type="eggNOG" id="ENOG502S5IW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096241_0_0_1"/>
    <dbReference type="OMA" id="SGMPVAC"/>
    <dbReference type="OrthoDB" id="1112980at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HPG0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062064">
        <property type="term" value="F:box C/D methylation guide snoRNP complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027921">
        <property type="entry name" value="NOPCHAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28674:SF1">
        <property type="entry name" value="NOP PROTEIN CHAPERONE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28674">
        <property type="entry name" value="SIMILAR TO DNA SEGMENT, CHR 10, WAYNE STATE UNIVERSITY 102,-EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15370">
        <property type="entry name" value="NOPCHAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK08747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="184" mass="19944" checksum="8A49EF8EF8258C61" modified="2012-06-13" version="1">MGEPSKDLLDVPSEPKQPFFIESLFAGKEQHNEQGKRKAGPPTDPLPKSQVLGKVKDFLGEMAKANEKLQLDAQNKPPQEYDIEALTGNEKEYIEMDLLLGVADLHSEQAVGAAEATMSGFPPSGRSYASSSSESEDGSDDSDEDSGDEPDMSNKVKCDDPAAQETHTAKGKKPIKRQKIVVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPZ4</accession>
    <name>I1HPZ4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100836868</name>
        <name evidence="12" type="ORF">BRADI_2g45390v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569474.1">
        <property type="nucleotide sequence ID" value="XM_003569426.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPZ4"/>
    <dbReference type="STRING" id="15368.I1HPZ4"/>
    <dbReference type="MEROPS" id="C26.964"/>
    <dbReference type="EnsemblPlants" id="KQK09000">
        <property type="protein sequence ID" value="KQK09000"/>
        <property type="gene ID" value="BRADI_2g45390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836868"/>
    <dbReference type="Gramene" id="KQK09000">
        <property type="protein sequence ID" value="KQK09000"/>
        <property type="gene ID" value="BRADI_2g45390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836868"/>
    <dbReference type="eggNOG" id="KOG2387">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_1"/>
    <dbReference type="OMA" id="DTHEAYQ"/>
    <dbReference type="OrthoDB" id="1739076at2759"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HPZ4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000305">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000012">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF13">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="313"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK09000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="610" mass="66453" checksum="DEDD390A58960845" modified="2012-06-13" version="1">MKYVVVTGGVVSGLGKGVTASSIGAVLKSCGLRVTTIKIDPYLNTDAGTMSPIEHGEVFVLDDGGEVDLDLGNYERFLDIKLTGDNNITTGKIYQAVIDKERRGDYLGKTVQVVPHITDEIQDWIERVAMNPVDGKEGPPDVCVIELGGTIGDIESMPFIEALGQFSYRVGTGNFCLVHVSLVPVLTVVGEQKTKPTQHSVRGLRGLGLAPDILACRSTEPLEEHVTAKLSQFCNVPISSIVNLHDVTNIWHIPLLLRDQKAHEAILKVLDLQLLGKVPREPKLAEWTERASKLDKLKTPVTIAVVGKYTGLSDSYLSIQKALLHASVVMERKLVVEWIPSCDLEDSAAKDTHEAYQKAWKLLKGADGVLVPGGFGDRGVQGKILAAKYARENNVPYLGICLGMQIAVIEFARSVMKLCSANSTEFDPATTSPCVIFMPEGSRTQMGATMRLGSRRTYFLGNNCKSGKLYGNASFVDERHRHRYEVNPEIVPELEKAGLSFVGKDESGSRMEIVELPSHDFFIGVQFHPEFKSRPGKPSPLFLGLIAAASGQLDLLLQRSSGVVSSNLAPRCITKGTTVPVIKVYPNGHIKKPLKSLVNGYYTNSNGIHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUI8</accession>
    <name>I1HUI8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF506 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100838073</name>
        <name evidence="1" type="ORF">BRADI_2g58590v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564912.1">
        <property type="nucleotide sequence ID" value="XM_003564864.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUI8"/>
    <dbReference type="STRING" id="15368.I1HUI8"/>
    <dbReference type="EnsemblPlants" id="KQK11174">
        <property type="protein sequence ID" value="KQK11174"/>
        <property type="gene ID" value="BRADI_2g58590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838073"/>
    <dbReference type="Gramene" id="KQK11174">
        <property type="protein sequence ID" value="KQK11174"/>
        <property type="gene ID" value="BRADI_2g58590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838073"/>
    <dbReference type="eggNOG" id="ENOG502QTYV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042726_1_1_1"/>
    <dbReference type="OMA" id="LREDSYG"/>
    <dbReference type="OrthoDB" id="548115at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01615">
        <property type="entry name" value="A_thal_3542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF84">
        <property type="entry name" value="F21O3.6 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK11174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="30674" checksum="74457FCBDBD59A59" modified="2012-06-13" version="1">MAVYIRLDDAVRARLRGAASGSTGSTTSSGSEHDASACLSGLVQAFLETENSAALEEDHPAPKGSGAADDDGSSASAAAAAAAAAMRELLDPPAAEDPFRIRLAAAVSAALEAEGSHGHVHGAALRRAMVRRLRAAGYDAGVCKSRWEASGGGLTAGAHEYVDVVFSPSAAPSTRYIVDPEFRAGMEVARATAEYAAVVAAVPSPAVVAREESLGRAVRVAADAARRSLRAQGLHVPPWRKSRYMLAKWLGPYKRSPASTASAPAMAVLTAAPAGMMVEGVKCRAVGFFPPAAAAARIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUK6</accession>
    <name>I1HUK6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100841718</name>
        <name evidence="2" type="ORF">BRADI_2g58750v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564924.1">
        <property type="nucleotide sequence ID" value="XM_003564876.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUK6"/>
    <dbReference type="STRING" id="15368.I1HUK6"/>
    <dbReference type="EnsemblPlants" id="KQK11204">
        <property type="protein sequence ID" value="KQK11204"/>
        <property type="gene ID" value="BRADI_2g58750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841718"/>
    <dbReference type="Gramene" id="KQK11204">
        <property type="protein sequence ID" value="KQK11204"/>
        <property type="gene ID" value="BRADI_2g58750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841718"/>
    <dbReference type="eggNOG" id="ENOG502QR8G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037686_0_0_1"/>
    <dbReference type="OMA" id="CFCKGVG"/>
    <dbReference type="OrthoDB" id="1925372at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35729">
        <property type="entry name" value="T1B9.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35729:SF1">
        <property type="entry name" value="T1B9.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK11204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="553" mass="60391" checksum="FEB56F456A560EFE" modified="2012-06-13" version="1">MGVWREGAGWCFCSGGGGGRSERVKAAIFSAKAAALAAVVGSGGHGTGLLLHRNLLLTTHGNLPSAAAAEDADALLGHARLPARLVPHRFFITSSILDLTIVGVEPTENESTLQGQQPHYLKTCCKPSLDHGSAVYLLGHTGKNELVIGEGKVVIGTDNLIKLSTDGVTWCPGSAGFDAQGNLAFMICDPMKLASSSPTARSSSASSSSSHSSKKDQPMQFGIPISVVCDWLYQHWQGNLDEVSKPKLPLVRLMSSRSDHSSTSFNRRHVFKPADDENDDASVSSSQVTSKPKYQQASGSPANARISHDANLLVDLRMNNEQGISTPEIYESPRGSSCQSQKDPAPIQLLDINFVPRAPKTIFLPLPLKQMLSDENNAETSKPRNQSRENCFPSGFIWHRNCEADSREPTVAPLQEDCSSEGQSSSSPAEQLGYRNQDQFSSEEETMYSAETMESRNIPSPREKHVGRSQSCVNYSRWSSPRKLSVQNGTLRKQHTLIPVRKTHSQSTSLPQRSHDYLSPTVSSAMKKRNSMEQQQPPKPRRIIVQSSPKWMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HVE5</accession>
    <name>I1HVE5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Gamma-secretase subunit PEN-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100821291</name>
        <name evidence="9" type="ORF">BRADI_2g61420v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PEN-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565118.1">
        <property type="nucleotide sequence ID" value="XM_003565070.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HVE5"/>
    <dbReference type="STRING" id="15368.I1HVE5"/>
    <dbReference type="EnsemblPlants" id="KQK11650">
        <property type="protein sequence ID" value="KQK11650"/>
        <property type="gene ID" value="BRADI_2g61420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821291"/>
    <dbReference type="Gramene" id="KQK11650">
        <property type="protein sequence ID" value="KQK11650"/>
        <property type="gene ID" value="BRADI_2g61420v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821291"/>
    <dbReference type="eggNOG" id="KOG3402">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124142_1_0_1"/>
    <dbReference type="OMA" id="WAINFCY"/>
    <dbReference type="OrthoDB" id="524898at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070765">
        <property type="term" value="C:gamma-secretase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019379">
        <property type="entry name" value="Gamma_Secretase_Asp_P_PEN2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16318">
        <property type="entry name" value="GAMMA-SECRETASE SUBUNIT PEN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16318:SF0">
        <property type="entry name" value="GAMMA-SECRETASE SUBUNIT PEN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10251">
        <property type="entry name" value="PEN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK11650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="17337" checksum="CFCD5F130E967A4C" modified="2012-06-13" version="1">MEARVSGVPEDEESGLLPRQSAAAAAGRRPSSSSAFPRRAPPPAVWATVDGPLGMPLEDAEGHARRFFLWGFACLPFLWAINFCYFWPVLRSPAASSPPAFASIRPYVVRSVIGFTIFAAVLLTWATTFIVGGERLFGPAWNDLVMYNVADKLGLTGFMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1HX75</accession>
    <name>I1HX75_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">W2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100831458</name>
        <name evidence="9" type="ORF">BRADI_3g03850v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574697.1">
        <property type="nucleotide sequence ID" value="XM_003574649.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HX75"/>
    <dbReference type="STRING" id="15368.I1HX75"/>
    <dbReference type="EnsemblPlants" id="KQJ93319">
        <property type="protein sequence ID" value="KQJ93319"/>
        <property type="gene ID" value="BRADI_3g03850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831458"/>
    <dbReference type="Gramene" id="KQJ93319">
        <property type="protein sequence ID" value="KQJ93319"/>
        <property type="gene ID" value="BRADI_3g03850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831458"/>
    <dbReference type="eggNOG" id="KOG2767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026663_1_1_1"/>
    <dbReference type="OMA" id="CKACMLD"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HX75">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11561">
        <property type="entry name" value="W2_eIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000046">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="8">
        <location>
            <begin position="305"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="143"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="269"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="147"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ93319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="454" mass="49143" checksum="FC8F04411C8AAB0B" modified="2012-06-13" version="1">MALQNIGASNKDDAFYRYKMPRMLTKIEGRGNGIKTNIVNMVDIAKALARPASYTTKYFGCELGAQSKFDEKTGISLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEVLISKTQMITLKCAACGFLSDVDMRDKLTTFILKNPPEQKKGGKDKKAMRRAEKERLKEGEAADEEMKKLKKDAKKKGGSSKESTAKGVSKKKAGGGSDEEHATSPTHSRDADFAASADDDDDNDVQWATDTSAEAAKKRMQEQLSAATAEMVMLATEETEKKKKQTLQKEASANGNGAAKDKDDSNGNQTGAKATPYDDLVEEIKATLGNAATTAQLKTLLSSSALSPKEVMDALFEALFHGVGKGFAKDVLKNKKFLAAAVPDEDAQMLLLQTIEAFCGKCSAEALKEVPVVLKALYDGDVLDEETIVQWYNEAVAAGKNSQVVKNAKPVVEWLQSAESDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1F6</accession>
    <name>I1I1F6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100823272</name>
        <name evidence="8" type="ORF">BRADI_3g16540v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234385.1">
        <property type="nucleotide sequence ID" value="XM_010236083.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1F6"/>
    <dbReference type="EnsemblPlants" id="KQJ95324">
        <property type="protein sequence ID" value="KQJ95324"/>
        <property type="gene ID" value="BRADI_3g16540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823272"/>
    <dbReference type="Gramene" id="KQJ95324">
        <property type="protein sequence ID" value="KQJ95324"/>
        <property type="gene ID" value="BRADI_3g16540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823272"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013137_18_2_1"/>
    <dbReference type="OMA" id="DLWALQP"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969:SF81">
        <property type="entry name" value="OS08G0157400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094991" description="RING-type domain-containing protein" evidence="6">
        <location>
            <begin position="41"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="111"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ95324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="184" mass="19268" checksum="6CD67C889DA76671" modified="2012-06-13" version="1">MGFPSVCYCVILPQPLILVLQLLDFLRHAVLLCLSSLGLAAPPTSDEHPAYAPPPDLWAEDSSSPSPSPSLGAAAMAMAAPADIKARLPAVRYADLRSRRGAGNAPAPACCAVCLGALEARHRVRELGNCAHAFHKACIDKWVDKGQATCPLCRALLLPAAEDAAGKLPLPLPLPHSSSSSFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2T6</accession>
    <name>I1I2T6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glabrous enhancer-binding protein-like DBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100828604</name>
        <name evidence="4" type="ORF">BRADI_3g20610v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GeBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571636.1">
        <property type="nucleotide sequence ID" value="XM_003571588.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2T6"/>
    <dbReference type="STRING" id="15368.I1I2T6"/>
    <dbReference type="EnsemblPlants" id="KQJ96045">
        <property type="protein sequence ID" value="KQJ96045"/>
        <property type="gene ID" value="BRADI_3g20610v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828604"/>
    <dbReference type="Gramene" id="KQJ96045">
        <property type="protein sequence ID" value="KQJ96045"/>
        <property type="gene ID" value="BRADI_3g20610v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828604"/>
    <dbReference type="eggNOG" id="ENOG502R6HP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039244_0_0_1"/>
    <dbReference type="OMA" id="KIAEVRM"/>
    <dbReference type="OrthoDB" id="662253at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007592">
        <property type="entry name" value="GEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053932">
        <property type="entry name" value="GeBP-like_DBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31662">
        <property type="entry name" value="BNAANNG10740D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31662:SF10">
        <property type="entry name" value="OS02G0288200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04504">
        <property type="entry name" value="GeBP-like_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glabrous enhancer-binding protein-like DBD" evidence="3">
        <location>
            <begin position="191"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ96045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="454" mass="49429" checksum="F3C179E87C24180C" modified="2012-06-13" version="1">MARKRRAPSPPPPPPPPQEESSSEETSSGEEEEVEPTPTRATQKPHEIPKSPPTATAADATAEGSDDESSDSEIDAEAFQLRQVVTSPSKPPAAASKLESDADEEQGEPSDKPEPLGKKKEMKPKAPSARKRQATESTPSGKSKKAKAEVEKAVPEPAPPPKAKKAKAGAENTKKAVPDPSPSSKSEKLKRWTLADEIKILEAFIGYVKTNGTQPGALDLIAAVGDTLDRKNCSKVEMYEKVRNLRQRYEKAVSTGTLPVKDDDLRKFKLSEVAWGANAKEVASASISQNDGAKGKKTQTTKEKIEGDTKGRSSKSKKQGKHIEELEDNETTDPQIGGTLVRSKREKSDKKMGGDVDSLGPKEATEEMDTAANVKRIRKGFDELQNLYPNLSTYVESIKAQHPCGETLKRAFELIDDEKACTLESKIKKQRVAEVKTEIRRADTKKEVANMLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1I333</accession>
    <name>I1I333_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Inositol-tetrakisphosphate 1-kinase</fullName>
            <ecNumber evidence="12">2.7.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100821944</name>
        <name evidence="17" type="ORF">BRADI_3g21470v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>1D-myo-inositol 1,3,4-trisphosphate + ATP = 1D-myo-inositol 1,3,4,5-tetrakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13253"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57895"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.159"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1D-myo-inositol 1,3,4-trisphosphate + ATP = 1D-myo-inositol 1,3,4,6-tetrakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20940"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57660"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.159"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>1D-myo-inositol 3,4,5,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12452"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57539"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.134"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12 14">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the ITPK1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.134" evidence="12"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573753.1">
        <property type="nucleotide sequence ID" value="XM_003573705.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I333"/>
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            <begin position="40"/>
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1I390</accession>
    <name>I1I390_BRADI</name>
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        <recommendedName>
            <fullName evidence="4">L-gulonolactone oxidase</fullName>
            <ecNumber evidence="4">1.1.3.8</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="14" type="primary">LOC100831264</name>
        <name evidence="13" type="ORF">BRADI_3g22060v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32363"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="EC" id="1.1.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; L-ascorbate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQJ96275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:membrane"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
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        <property type="entry name" value="FAD-bd_PCMH"/>
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        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
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        <property type="entry name" value="FAD-linked_Oxidoreductases_BP"/>
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        <property type="entry name" value="GULLO2-like_C"/>
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        <property type="entry name" value="GULO_Plant"/>
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        <property type="entry name" value="Oxid_FAD_bind_N"/>
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        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
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        <property type="entry name" value="pln_FAD_oxido"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878">
        <property type="entry name" value="GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALO"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FAD_binding_4"/>
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        <property type="entry name" value="GULLO2-like_3rd"/>
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        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0060">Ascorbate biosynthesis</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095006" description="L-gulonolactone oxidase" evidence="11">
        <location>
            <begin position="21"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="12">
        <location>
            <begin position="49"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ96275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="589" mass="63630" checksum="186560EFA6DBECF1" modified="2012-06-13" version="1">MESLLPVLFLGLLLLHHAGCSPLPDPVVCTRGTSNCTVSNTYGSFTDRTVCHAANVTYPSTEEELVAAVAAVASAKRKAKVATRHSHSIPKLACPGGSDGTIISTARLNRTVRIDVAKRLMTVESGVVLRDLITAAAEAGLSLPHSPYWYGLTIGGLLATGAHGSSLWGKGGAVHEYVVGLRIVTPAPASQGFAVVRELGADHPDLDAAKVSIGVLGVVSQVTLALQPLFKRSVTFVKRDDSDLANQAVVWGRLHEFGDMTWRPQEGKVLYRQDDRVDISSPGNGLNDLIIFRPRPTRGLIDARAAEERLQENGTDKAYCAAVRAQAATTEQLAYGFTNDGISFTGFPVVGYQHRIQASGTCLNGPEDGLLTSCTWDPRIRGSFYYNSGFSVALSKAPAFIADMQRLRDINPDAFCAGIDARVGVVLRYIKASSAYLGKPEDSIDFDVVFYRSHTEGEPRVHGDVVDEIEQIALLKYGGLPHWGKNRNFAFDGAIRKYPKAAEFLRMKERYDPDGIFSSEWSDQVLGISGSTNIVKKGCAVEGLCICSEDSHCAPDQGYFCRPGKVYTKARVCSFQPTGHAHPRSGIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6K1</accession>
    <name>I1I6K1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100827689</name>
        <name evidence="10" type="ORF">BRADI_3g34567v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WRKY group II-e family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572136.1">
        <property type="nucleotide sequence ID" value="XM_003572088.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6K1"/>
    <dbReference type="EnsemblPlants" id="PNT67981">
        <property type="protein sequence ID" value="PNT67981"/>
        <property type="gene ID" value="BRADI_3g34567v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827689"/>
    <dbReference type="Gramene" id="PNT67981">
        <property type="protein sequence ID" value="PNT67981"/>
        <property type="gene ID" value="BRADI_3g34567v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827689"/>
    <dbReference type="eggNOG" id="ENOG502QU8N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067677_0_0_1"/>
    <dbReference type="OMA" id="HRSTIIV"/>
    <dbReference type="OrthoDB" id="1937086at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I6K1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000005">
        <property type="entry name" value="probable WRKY transcription factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096:SF108">
        <property type="entry name" value="WRKY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="9">
        <location>
            <begin position="176"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="265"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="265"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT67981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="352" mass="38503" checksum="6D371052432FF7BE" modified="2012-06-13" version="1">MADMIADLFWLSPGEQGDLSDVVRASLHPQPEPEHYYQQLPAAAASFSYLQQPEELAEGGLQLHGDHGQLGIMTDDASSKRPSSSDHKQVISPDYSLAEMIHGMSPPQRPPGMERLQEAAGDDVVPERSDRMRQHMLSMDHHPARVPAASKPRKSQTKRVVCIPAPTAASGRQSTSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRTDPNMLVITYTSDHNHPWPTQRNALAGSTRLSSSASAIAAAAKNTSSSSSSSLPTAAQGPSRNSDSINNNDPAAAAAHHRQLKQEDDLDLFADMDSLNILSSITKTHEEDSKQQQQLFDPFSSCFFDCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8E7</accession>
    <name>I1I8E7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PsbP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100843794</name>
        <name evidence="3" type="ORF">BRADI_3g39830v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574737.1">
        <property type="nucleotide sequence ID" value="XM_003574689.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8E7"/>
    <dbReference type="FunCoup" id="I1I8E7">
        <property type="interactions" value="534"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I8E7"/>
    <dbReference type="EnsemblPlants" id="KQJ98897">
        <property type="protein sequence ID" value="KQJ98897"/>
        <property type="gene ID" value="BRADI_3g39830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843794"/>
    <dbReference type="Gramene" id="KQJ98897">
        <property type="protein sequence ID" value="KQJ98897"/>
        <property type="gene ID" value="BRADI_3g39830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843794"/>
    <dbReference type="eggNOG" id="ENOG502QUTY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089114_0_0_1"/>
    <dbReference type="OMA" id="PTPEWNK"/>
    <dbReference type="OrthoDB" id="1916780at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009654">
        <property type="term" value="C:photosystem II oxygen evolving complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048564">
        <property type="term" value="P:photosystem I assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002683">
        <property type="entry name" value="PsbP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407:SF20">
        <property type="entry name" value="THYLAKOID LUMENAL 19 KDA PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01789">
        <property type="entry name" value="PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PsbP C-terminal" evidence="2">
        <location>
            <begin position="102"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ98897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="248" mass="26477" checksum="47671DC1712675CB" modified="2012-06-13" version="1">MTILASLLSPSPPLTTSPHPAPAQSAAVRLLAPSARPPFPATLAATAAAGLLLLSPLTPPPSHAAAADPDPSSFKVYYGTAASAANYGGYGGNASKKDAAEYVYDVPGTWKERLVSKVEKGTNGTDSEFYNPRKRSEREYLTFLSGFRALAPIGAVLDNLALSDVGLQDQISTADEVRSTEERKEDGQVYYEYEVAGAGAHSLISVTCARNKLYAHFVTAPNPEWGRDEEVLRRLHQSFKTVDFSGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICC0</accession>
    <name>I1ICC0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100838009</name>
        <name evidence="4" type="ORF">BRADI_3g50900v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572782.1">
        <property type="nucleotide sequence ID" value="XM_003572734.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICC0"/>
    <dbReference type="STRING" id="15368.I1ICC0"/>
    <dbReference type="EnsemblPlants" id="KQK00656">
        <property type="protein sequence ID" value="KQK00656"/>
        <property type="gene ID" value="BRADI_3g50900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838009"/>
    <dbReference type="Gramene" id="KQK00656">
        <property type="protein sequence ID" value="KQK00656"/>
        <property type="gene ID" value="BRADI_3g50900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838009"/>
    <dbReference type="eggNOG" id="ENOG502S26K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055715_2_1_1"/>
    <dbReference type="OMA" id="HIEMASN"/>
    <dbReference type="OrthoDB" id="696558at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd01958">
        <property type="entry name" value="HPS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.110.10:FF:000003">
        <property type="entry name" value="pEARLI1-like lipid transfer protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027923">
        <property type="entry name" value="Hydrophob_seed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051636">
        <property type="entry name" value="Plant_LTP/defense-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31731">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14547">
        <property type="entry name" value="Hydrophob_seed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095196" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="3">
        <location>
            <begin position="41"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK00656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="123" mass="12378" checksum="AA3051C9997B6AD4" modified="2012-06-13" version="1">MAPKTALLLLALSLVLLFTVASACGDSCPTPATPSYYDGKCPKNALKLAACADVLGLVSAEVGHPPAEPCCSILGGLADLEAAVCLCTAIKANVLGISLDIPVKLSLLVNYCGKSLPSGFICG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDE8</accession>
    <name>I1IDE8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anthocyanin acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100825626</name>
        <name evidence="3" type="ORF">BRADI_3g53980v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572896.1">
        <property type="nucleotide sequence ID" value="XM_003572848.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDE8"/>
    <dbReference type="STRING" id="15368.I1IDE8"/>
    <dbReference type="EnsemblPlants" id="KQK01128">
        <property type="protein sequence ID" value="KQK01128"/>
        <property type="gene ID" value="BRADI_3g53980v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825626"/>
    <dbReference type="Gramene" id="KQK01128">
        <property type="protein sequence ID" value="KQK01128"/>
        <property type="gene ID" value="BRADI_3g53980v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825626"/>
    <dbReference type="eggNOG" id="ENOG502QPXT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014546_7_2_1"/>
    <dbReference type="OMA" id="EMERFAC"/>
    <dbReference type="OrthoDB" id="1862401at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051504">
        <property type="entry name" value="Plant_metabolite_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31625">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK01128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="49689" checksum="AAA6E4341F22F18C" modified="2012-06-13" version="1">MAPAPSSFQHVTVLSRCEVSPSPPAPGPRALPLTFFDLVFWGFPPVQRLFFYDDPAGLVDVPGFLAHDLPLFRDSLAAALHRFYPLAGKLPCELPPEGPPEVVCSDGDSVRLTVAVAGEDDDFRDLAGDHPRDTARLRPLLPPLFEHGGCRDVFAVQLTVFPRAGICVGTTLHHAVADGSGYAHFIRTWAVFHRLGPKKKNAIDVAPPLLDRGAVRDDGGRLREAFLRDHRALAAAGDTRLGDWDLSVQGAGGVQLATFRFTGELLRKLGGRVESETSARGCSPYAVACGAAWAGIAHARGGIGVGIGKEQQFGFVTGCKPRANPPVPASYFGNCLGLCRVRVEEQEGKELTASVASAAIWREIEGLAEPGRALRGARGWVRLVRDSAAARAVTVAGSPKLGMYAAADMGGAPWGRPRKVEIASVERTGALALAEDGGGIEVGVALPRAEMEAFQSFFSALVASCDSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDJ8</accession>
    <name>I1IDJ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Heparanase-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100837386</name>
        <name evidence="12" type="ORF">BRADI_3g54370v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Lysosome membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 79 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570329.1">
        <property type="nucleotide sequence ID" value="XM_003570281.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDJ8"/>
    <dbReference type="FunCoup" id="I1IDJ8">
        <property type="interactions" value="206"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01193">
        <property type="protein sequence ID" value="KQK01193"/>
        <property type="gene ID" value="BRADI_3g54370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837386"/>
    <dbReference type="Gramene" id="KQK01193">
        <property type="protein sequence ID" value="KQK01193"/>
        <property type="gene ID" value="BRADI_3g54370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837386"/>
    <dbReference type="eggNOG" id="ENOG502QQST">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021823_3_0_1"/>
    <dbReference type="OMA" id="CTPFTKN"/>
    <dbReference type="OrthoDB" id="726732at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IDJ8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004566">
        <property type="term" value="F:beta-glucuronidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000023">
        <property type="entry name" value="heparanase-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005199">
        <property type="entry name" value="Glyco_hydro_79"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363:SF17">
        <property type="entry name" value="HEPARANASE-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363">
        <property type="entry name" value="HEPARANASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03662">
        <property type="entry name" value="Glyco_hydro_79n"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095222" description="Heparanase-like protein 3" evidence="11">
        <location>
            <begin position="26"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK01193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="58412" checksum="1070790447A858D2" modified="2012-06-13" version="1">MAAGLLLQVVGFCVWAVFSLGGSSATVSSTSAAAAAAGGALVVVDARSAIAETEQAFVCATLDWWPPEKCDYGTCAWGLASLLNLNLNNKILLNAVKAFSPLKLRLGGSLQDVLVYGTGDGPEPCTPFTFNSSVMFSFSQGCLPMHRWDELNAFFKKSGAQIIFGLNALNGRVPMPDGSLGGPWNYTNAASFIRYTVSKGYDIHGWELGNELSGSGVGARVGADQYAADVITLHQIIDKTYQGPKPLVIAPGGFFDAAWFNELVSKTKPNQMDVITHHIYNLGPGVDTHLIEKILDPSYLDGEASTFSNLQGILKSAGTSTVAWVGESGGAYNSGHHLVTDAFVFSFWYLDQLGMSAKYDTKSYCRQTLVGGNYGLLNTTTFEPNPDYYSALLWHRLMGTKVLSTTFNGTNKIRAYTHCARDTEGITLLLINLSGNDTNHIYVTSEGAHLYSAKKENRRFAHVPGLGEVAEFKRQEYHLTPKDGNLQSQHMLLNGNLLATDANGDIPKLEPVQVEGSQPITVGPYSIVFAHIPSFYAPACM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDR7</accession>
    <name>I1IDR7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100822439</name>
        <name evidence="3" type="ORF">BRADI_3g54950v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDR7"/>
    <dbReference type="FunCoup" id="I1IDR7">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IDR7"/>
    <dbReference type="EnsemblPlants" id="KQK01290">
        <property type="protein sequence ID" value="KQK01290"/>
        <property type="gene ID" value="BRADI_3g54950v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01290">
        <property type="protein sequence ID" value="KQK01290"/>
        <property type="gene ID" value="BRADI_3g54950v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_0_1"/>
    <dbReference type="OMA" id="CILESIM"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000219">
        <property type="entry name" value="Cinnamoyl-CoA reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF353">
        <property type="entry name" value="CINNAMOYL-COA REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK01290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="347" mass="37733" checksum="ED2FD1E7CFF8BCA4" modified="2012-06-13" version="1">MPSIATKASGNNGEIKQQQKELVCVTGAGGFIGSWVVKELLLRGYRVRGTARDPADRKNAHLLALEGAEARLTLCRADVLDYDGLRAAFAGCHGVFHVASPVSNDPDLAPVAVEGTRNVINAAADAGVRRVVFTSSYGAVHMDPKRSPDAVLDESCWSDYEFCKQTGNLYCCAKMMAEITATEEAARRGLELAVVVPSMTMGPMLQQQLNFSSGHVARYLTGAKAAYPDAVAAYVDVRDVARAHVLAYERAAAANGQRFLCIGAVLHRASFLQLLKELFPQFPVTAKCQVDGEPMAKPYRFSNQRLKDLGLEFTPLRESLYETVMSLQQYGHLPQLPAPVAPKRAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDW6</accession>
    <name>I1IDW6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g55340v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDW6"/>
    <dbReference type="FunCoup" id="I1IDW6">
        <property type="interactions" value="2796"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IDW6"/>
    <dbReference type="EnsemblPlants" id="KQK01351">
        <property type="protein sequence ID" value="KQK01351"/>
        <property type="gene ID" value="BRADI_3g55340v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01351">
        <property type="protein sequence ID" value="KQK01351"/>
        <property type="gene ID" value="BRADI_3g55340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831041"/>
    <dbReference type="eggNOG" id="KOG2865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_4_1"/>
    <dbReference type="OMA" id="PEDQFTN"/>
    <dbReference type="OrthoDB" id="275457at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05271">
        <property type="entry name" value="NDUFA9_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000326">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 39 kD subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="79"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK01351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="412" mass="44717" checksum="962C768860AF18BC" modified="2012-06-13" version="1">MQAAAWRRQLLGQNQLSPASSAAIAAFRSASQPALAPQGFGGVDGARYMSARTPAVKGAGHLVRKGTGGRSSVSGIVATVFGATGFLGRYVVQQLAKMGSQVLVPFRGCEDSHRHLKLMGDLGQIVPMKYNPRDVDSIKTAVAKSNVVINLIGREYETRNYGFEEVNHHMAEQLAMISKEHGGIVRFIQVSSLGASSSSPSRMLRAKAAGEESVLKEFPEATIMRPATLIGTEDRILNRWAMYAKNWGFLPLMGGGSTKIQPVYVVDVAAAIVNSLKDDGTSMGKTYELGGPDIYTVHDLAELMFETIREWPRYVNVPFPIARAIASPREMLLNKVPFPLPTPSIFNLDQINALSVDNLVSENALTFADLEIRPHKLKGYPVEFLVCYRKGGPSFGSTVSEKMGSSDVAPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEK9</accession>
    <name>I1IEK9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Endoglucanase</fullName>
            <ecNumber evidence="9">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100838698</name>
        <name evidence="11" type="ORF">BRADI_3g57190v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="9"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEK9"/>
    <dbReference type="FunCoup" id="I1IEK9">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IEK9"/>
    <dbReference type="EnsemblPlants" id="KQK01627">
        <property type="protein sequence ID" value="KQK01627"/>
        <property type="gene ID" value="BRADI_3g57190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838698"/>
    <dbReference type="Gramene" id="KQK01627">
        <property type="protein sequence ID" value="KQK01627"/>
        <property type="gene ID" value="BRADI_3g57190v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838698"/>
    <dbReference type="eggNOG" id="ENOG502QQ06">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008926_1_4_1"/>
    <dbReference type="OMA" id="PSWRGDS"/>
    <dbReference type="OrthoDB" id="10257085at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000020">
        <property type="entry name" value="Endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00592">
        <property type="entry name" value="GH9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 9" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982804" description="Endoglucanase" evidence="9">
        <location>
            <begin position="30"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="10">
        <location>
            <begin position="43"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK01627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="502" mass="54511" checksum="CD2AF7BE1795667A" modified="2012-06-13" version="1">MKNKRAPASGGVRTVALAILAALLVVVSGAVQAAAGAAVSFNYKDALSKSIMFLEAQRSGKLPPNNRIKWRGDSGMEDGKLAHVDLTGGYYDAGDNVKYGLPLAFTVTTLAWTALAFKPELQAAGELNNVHAAIRWGTDYFLKCAAKKNHMYVQVGDPNLDHQCWVRPENMNTPRTLYEIDDKTPGTEIAAETAAALAASSLVFRDTDKGYSRRLLNKAKLLYQFGRSHMKTYDGECPFYCSYSGYNDELLWAATWLYMATKRQAYADFISHEAISSSVAEFSWDLKYPGAQVLLADLNMTANGGLQSFKTQADNFVCAVLPDTPFHQVFITPGGMIHLRDGANTQYVTGTAFLFVVYSDILLRSGQQVMCGSQPIPPARLKEFAKQQIDYLLGANPRGRSYVVGFGVNPPTQPHHRGASTPVLKPGTVVNCGMSFGDWFAPDRPNPNELTGAIMGGPDGNDEFVDKRNASSCTEPCTYINSLSIGPLAALAVRGNDLVASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IIM3</accession>
    <name>I1IIM3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100846944</name>
        <name evidence="3" type="ORF">BRADI_4g07980v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575553.1">
        <property type="nucleotide sequence ID" value="XM_003575505.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IIM3"/>
    <dbReference type="EnsemblPlants" id="KQJ86832">
        <property type="protein sequence ID" value="KQJ86832"/>
        <property type="gene ID" value="BRADI_4g07980v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846944"/>
    <dbReference type="Gramene" id="KQJ86832">
        <property type="protein sequence ID" value="KQJ86832"/>
        <property type="gene ID" value="BRADI_4g07980v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846944"/>
    <dbReference type="eggNOG" id="ENOG502QQ23">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="QMRNNIA"/>
    <dbReference type="OrthoDB" id="1923031at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IIM3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33825">
        <property type="entry name" value="CHITINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33825:SF14">
        <property type="entry name" value="CHITINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ86832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="26393" checksum="62673EAF1FC14A72" modified="2012-06-13" version="1">MAGGQQLHGLLCLPPPRPVLPISARPPPCRARPPLASLRILEPSPPVPKKRPSSATAAKSKAAAVLEDENGAREEAQEEEEEEEGYVATVGSGDIAGVPARHLAAARAGLGDPVFYLLTFVAITTSLAFTGMVAVAIPTMLAMRRAANSFTQLADAALEELPSTMAAVRLSGMEISDLTLELSDLSQEIADGVNKSANIVQAVEDGIGQMRNIARQQTTSMIQERANLRTIPTAGPAKKSNESLNRRQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1IIT9</accession>
    <name>I1IIT9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100829731</name>
        <name evidence="9" type="ORF">BRADI_4g08520v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_4g08520v3"/>
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    <dbReference type="GeneID" id="100829731"/>
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        <property type="gene ID" value="BRADI_4g08520v3"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="term" value="P:transmembrane transport"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
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    </dbReference>
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        <property type="entry name" value="S04_transporter_CS"/>
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    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
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        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
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        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="186"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="198"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="244"/>
            <end position="262"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="454"/>
            <end position="484"/>
        </location>
    </feature>
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        <location>
            <begin position="509"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="665"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="676"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ86925"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1ILJ0</accession>
    <name>I1ILJ0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carboxypeptidase</fullName>
            <ecNumber evidence="11">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100835772</name>
        <name evidence="12" type="ORF">BRADI_4g17900v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable carboxypeptidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="11"/>
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        <property type="protein sequence ID" value="KQJ88430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003577481.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ILJ0"/>
    <dbReference type="MEROPS" id="S10.005"/>
    <dbReference type="EnsemblPlants" id="KQJ88430">
        <property type="protein sequence ID" value="KQJ88430"/>
        <property type="gene ID" value="BRADI_4g17900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835772"/>
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        <property type="protein sequence ID" value="KQJ88430"/>
        <property type="gene ID" value="BRADI_4g17900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835772"/>
    <dbReference type="eggNOG" id="KOG1282">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="OMA" id="VANKLWV"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF281">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982821" description="Carboxypeptidase" evidence="11">
        <location>
            <begin position="29"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ88430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="468" mass="52013" checksum="108F48B83F359F95" modified="2012-06-13" version="1">MPSLLYCSKPLAMVSLLLIAALCHLGSCNGGGRGDRITKLPGQPEVSFGQYSGYIGVDGKGSRALFYYFVEAELDPISKPLVLWLNGGPGCSSLGVGAFSENGPFRPSGQVLVKNEYSWNKEANVIYLETPAGVGYSYSADAAYYQGVNDKMTAMDNMVFMQRWLEKFPQYKGRELYIAGESYAGHYIPQLAEVMVEFNKNEKIFNLKGLALGNPVLEFTTDFNSRAEYFWSHGLISDSTYRIFTSVCNYSQYVSEYYGGSLSPLCARVMNQVTRETSRFVDKYDVTLDVCLASVLSQSMILSPHRHVGQRIDVCIEDETVNYLNRKDVQEALHAKLIGVKNWAVCSSVLQYELLNLQIPTINIVGSLVKSGIRVLVYSGDQDSVIPLTGSRTLVQNLAHDLGLKTSTPYRVWFEGKQVGGWTQVYGDMLSFATIRGASHEAPFSQPKRSLVLYRAFLQGRPLPENFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMA0</accession>
    <name>I1IMA0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100822482</name>
        <name evidence="9" type="ORF">BRADI_4g21220v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577657.1">
        <property type="nucleotide sequence ID" value="XM_003577609.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IMA0"/>
    <dbReference type="FunCoup" id="I1IMA0">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IMA0"/>
    <dbReference type="EnsemblPlants" id="KQJ88791">
        <property type="protein sequence ID" value="KQJ88791"/>
        <property type="gene ID" value="BRADI_4g21220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822482"/>
    <dbReference type="Gramene" id="KQJ88791">
        <property type="protein sequence ID" value="KQJ88791"/>
        <property type="gene ID" value="BRADI_4g21220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822482"/>
    <dbReference type="eggNOG" id="ENOG502QSV3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025359_1_2_1"/>
    <dbReference type="OMA" id="NIMQRCN"/>
    <dbReference type="OrthoDB" id="1728340at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="22"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="198"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="359"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ88791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="389" mass="41849" checksum="CBA1F32207165A71" modified="2012-06-13" version="1">MADVEARRVCGMPEKMQLHVAMLSLQFGYAGFHVVSRLALNMGISKLVFPVYRNIIALILLVPFAYFLEKKDRPQLTLNFVIQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFAMAAALRIEKVRLDRRDGVAKVVGTLACVAGASVITLYKGPTIFGPGGGDKLMSMGRPEVSWTAALAGEGKNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPARLSVTSYTCFFGVIQFLVIAAFLERDAEAWVFHSGSEIFTILYAGFIASGVAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASLTLGEKFYLGGIIGAALIITGLYLVLWGKSEERARMGKEAALMAATASNSSGGDHVARSTKASSITQPLLPSSTSSDNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITR5</accession>
    <name>I1ITR5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100843720</name>
        <name evidence="10" type="ORF">BRADI_4g40640v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578815.1">
        <property type="nucleotide sequence ID" value="XM_003578767.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITR5"/>
    <dbReference type="FunCoup" id="I1ITR5">
        <property type="interactions" value="508"/>
    </dbReference>
    <dbReference type="MEROPS" id="C01.104"/>
    <dbReference type="EnsemblPlants" id="KQJ91932">
        <property type="protein sequence ID" value="KQJ91932"/>
        <property type="gene ID" value="BRADI_4g40640v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843720"/>
    <dbReference type="Gramene" id="KQJ91932">
        <property type="protein sequence ID" value="KQJ91932"/>
        <property type="gene ID" value="BRADI_4g40640v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843720"/>
    <dbReference type="eggNOG" id="KOG1543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012184_1_0_1"/>
    <dbReference type="OMA" id="IYFEPRC"/>
    <dbReference type="OrthoDB" id="10253408at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000023">
        <property type="entry name" value="Senescence-specific cysteine protease SAG39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982834" evidence="7">
        <location>
            <begin position="24"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="9">
        <location>
            <begin position="37"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="8">
        <location>
            <begin position="123"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ91932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="339" mass="36612" checksum="C4778A615148F45E" modified="2012-06-13" version="1">MAIPKASILAILGCLCFCSSVLAARELNDDLSMAARHETWMAQYGRVYKDAAEKAQKFEVFKANARFIDSFNAENHKFWLGINQFADLTNEEFKATKTNKGFISNKARVSTGFKYENLKIEALPTSIDWRTKGAVTPVKDQGQCGCCWAFSAVAATEGIVKLSTGKLVSLSEQELVDCDVHGEDQGCEGGLMDDAFKFIITNGGLTQESSYPYDAEDGKCKSGSKSAGTIKSYEDVPANNEGALMKAVANQPVSVAVDGGDMTFQFYSGGVMTGSCGTDLDHGIAAIGYGVTSDGTKFWLMKNSWGTTWGENGFLRMEKDIADKKGMCGLAMEPSYPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITX6</accession>
    <name>I1ITX6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartic proteinase Asp1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucellin-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100827218</name>
        <name evidence="13" type="ORF">BRADI_4g41210v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238658.1">
        <property type="nucleotide sequence ID" value="XM_010240356.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITX6"/>
    <dbReference type="FunCoup" id="I1ITX6">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="MEROPS" id="A01.A27"/>
    <dbReference type="EnsemblPlants" id="KQJ92016">
        <property type="protein sequence ID" value="KQJ92016"/>
        <property type="gene ID" value="BRADI_4g41210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827218"/>
    <dbReference type="Gramene" id="KQJ92016">
        <property type="protein sequence ID" value="KQJ92016"/>
        <property type="gene ID" value="BRADI_4g41210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827218"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="CVDQMCA"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ITX6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05475">
        <property type="entry name" value="nucellin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000027">
        <property type="entry name" value="Aspartic proteinase Asp1 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000015">
        <property type="entry name" value="Aspartyl protease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033823">
        <property type="entry name" value="Nucellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF800">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095534" description="Aspartic proteinase Asp1" evidence="11">
        <location>
            <begin position="26"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="12">
        <location>
            <begin position="58"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ92016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="451" mass="49037" checksum="74863433BB9A9FCC" modified="2012-06-13" version="1">MSARWGPPAGLVLLLLLLSAVAARADRPARAGKAAEAEPEESSAVFQLYGDVYPHGLYYVAMSIGNPPRPYFLDVDTGSDLTWLQCDAPCVSCNKVPHPLYRPTKNKIVPCVDQLCSSLHGGLSGKHKCDSPKQQCDYEIKYADQGSSLGVLLTDSFAVRLANSSIVRPSLAFGCGYDQQVGSSTEVAPTDGVLGLGSGSISLLSQLKQHGITKNVVGHCLSIRGGGFLFFGDNLVPYSRATWVPMVRSAFKNYYSPGTASLYFGGRSLGVRPMEVVLDSGSSFTYFGAQPYQALVTALKSDLSKTLKEVFDPSLPLCWKGKKPFKSVLDVKKEFKSLVLSFSNGKKALMEIPPENYLIVTKFGNACLGILNGSEIGLKDLNIVGDITMQDQMVIYDNERGQIGWIRAPCDRIPNENTIHGFEEGYCWPQFPGIIGIQNEECAANYRSNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVF8</accession>
    <name>I1IVF8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATG8-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100825367</name>
        <name evidence="3" type="ORF">BRADI_5g00580v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579746.1">
        <property type="nucleotide sequence ID" value="XM_003579698.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239596.1">
        <property type="nucleotide sequence ID" value="XM_010241294.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVF8"/>
    <dbReference type="STRING" id="15368.I1IVF8"/>
    <dbReference type="EnsemblPlants" id="KQJ81412">
        <property type="protein sequence ID" value="KQJ81412"/>
        <property type="gene ID" value="BRADI_5g00580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825367"/>
    <dbReference type="Gramene" id="KQJ81412">
        <property type="protein sequence ID" value="KQJ81412"/>
        <property type="gene ID" value="BRADI_5g00580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825367"/>
    <dbReference type="eggNOG" id="KOG1347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054659_0_0_1"/>
    <dbReference type="OMA" id="VQFYDEG"/>
    <dbReference type="OrthoDB" id="604034at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040304">
        <property type="entry name" value="ATG8-IP-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34797">
        <property type="entry name" value="ATG8-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34797:SF1">
        <property type="entry name" value="ATG8-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ81412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="25855" checksum="85C02607364269CB" modified="2012-06-13" version="1">MSDNEKDVAEGATSRGADWEVVTLTASAYAAAPGPGGTEGGPAAESKGLDTSKDGRGSSNALLMSDHFVFPPSEHENLPIETAIKEIAPEKDVQEESTSVEDDSFKNVGRSYDVGSERIQYYDEGKNLSVDEAELKGDIAEQGSFHAEDDRHGSVVHDDDNDDGDKSDKPSDSKSRDSGPPCQCWLKKHMSCLYDQAKETNALWSVVVAAALVGLVILWRKDKLHLNCLKWRSRSAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZ83</accession>
    <name>I1IZ83_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF642 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100841904</name>
        <name evidence="8" type="ORF">BRADI_5g14470v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580051.1">
        <property type="nucleotide sequence ID" value="XM_003580003.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZ83"/>
    <dbReference type="STRING" id="15368.I1IZ83"/>
    <dbReference type="EnsemblPlants" id="KQJ83354">
        <property type="protein sequence ID" value="KQJ83354"/>
        <property type="gene ID" value="BRADI_5g14470v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841904"/>
    <dbReference type="Gramene" id="KQJ83354">
        <property type="protein sequence ID" value="KQJ83354"/>
        <property type="gene ID" value="BRADI_5g14470v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841904"/>
    <dbReference type="eggNOG" id="ENOG502QRP6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040251_0_0_1"/>
    <dbReference type="OMA" id="GPNKSEM"/>
    <dbReference type="OrthoDB" id="602524at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006946">
        <property type="entry name" value="DGR2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052437">
        <property type="entry name" value="Pectin_Meth_Modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31265:SF46">
        <property type="entry name" value="DUF642 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31265">
        <property type="entry name" value="OS02G0527500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04862">
        <property type="entry name" value="DUF642"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095652" description="DUF642 domain-containing protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="DUF642" evidence="7">
        <location>
            <begin position="27"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="DUF642" evidence="7">
        <location>
            <begin position="195"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ83354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="373" mass="40080" checksum="02386D8D08443C2C" modified="2012-06-13" version="1">MTKSRRCMALLLLVGMAAPAVLAVTDGLLPNGRFSQGPDKSQMNGTVVTGKHAMPNWELSGFVEYIESGHKEADMLLAVPVGANAVRLGNDATIRQQLSVTRKAYYSITFVAARSCAQAEKLNVSVDPEFGVLPIQTVYTSTGWDSYSWAFKARRSTVTLSIHNTGIEEDPACGPLIIAVAIKALALPQRIKGNMLRNGDFELGPYIFPDTPWGVMVPPILEDVHSPLPGWMIMSHTKVVKYVDTPHHAVPHGAYAVELVAGRESALVQEVGTVEGWTYRLSFFVGDAGNGCTGSLAVEVYAAGASLRAAYESDGKGGSKPFKLQFTAVGNSTRVVFQSSNHHMKSDATLCGPVVDNVSLVGVRVPAPRRLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1J051</accession>
    <name>I1J051_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)H-quinone oxidoreductase subunit M, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">NAD(P)H dehydrogenase subunit M</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NADH-plastoquinone oxidoreductase subunit M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100822606</name>
        <name evidence="17" type="ORF">BRADI_5g17027v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42608"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the chloroplast NDH complex, composed of a mixture of chloroplast and nucleus encoded subunits. Component of the NDH subcomplex A, at least composed of ndhH, ndhI, ndhJ, ndhK, ndhL, ndhM, ndhN and ndhO.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast thylakoid membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Stromal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDH complex subunit M family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580241.1">
        <property type="nucleotide sequence ID" value="XM_003580193.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J051"/>
    <dbReference type="STRING" id="15368.I1J051"/>
    <dbReference type="EnsemblPlants" id="KQJ83821">
        <property type="protein sequence ID" value="KQJ83821"/>
        <property type="gene ID" value="BRADI_5g17027v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822606"/>
    <dbReference type="Gramene" id="KQJ83821">
        <property type="protein sequence ID" value="KQJ83821"/>
        <property type="gene ID" value="BRADI_5g17027v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822606"/>
    <dbReference type="eggNOG" id="ENOG502QUN1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089187_0_0_1"/>
    <dbReference type="OMA" id="YMASTHF"/>
    <dbReference type="OrthoDB" id="2013483at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J051">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018922">
        <property type="entry name" value="NdhM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36900">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT M, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36900:SF1">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT M, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10664">
        <property type="entry name" value="NdhM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0618">Plastoquinone</keyword>
    <keyword evidence="6" id="KW-0874">Quinone</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="17"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ83821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="212" mass="23965" checksum="7835850D1826B6A8" modified="2012-06-13" version="1">MATAAASSFLSPAKFALQSRSPSLKPARGVRFVPARAQQQVKEQDPEATLPPPQEAAEEPAARAKRKGPAVSLPRQPLAESKNMGREYGSQWLSCTTRHVRIYAAYIDPETHAFDQTQTDKLTLILDPTEEFVWNDDTCQMVYNEFQDLVDHYEGAPLSEYTLRLIGSDLEHYIRKLLYDGDIKYNMNSRVLNFSMGKPRVKFNSSQIPDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1J182</accession>
    <name>I1J182_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100845148</name>
        <name evidence="3" type="ORF">BRADI_5g20090v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580482.1">
        <property type="nucleotide sequence ID" value="XM_003580434.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J182"/>
    <dbReference type="EnsemblPlants" id="KQJ84322">
        <property type="protein sequence ID" value="KQJ84322"/>
        <property type="gene ID" value="BRADI_5g20090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845148"/>
    <dbReference type="Gramene" id="KQJ84322">
        <property type="protein sequence ID" value="KQJ84322"/>
        <property type="gene ID" value="BRADI_5g20090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845148"/>
    <dbReference type="HOGENOM" id="CLU_037069_1_0_1"/>
    <dbReference type="OrthoDB" id="592687at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J182">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056594">
        <property type="entry name" value="AT5G49610-like_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33207">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23635">
        <property type="entry name" value="Beta-prop_AT5G49610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="23"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="F-box protein AT5G49610-like beta-propeller" evidence="2">
        <location>
            <begin position="121"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ84322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="408" mass="45847" checksum="1AE2521E9A8122E6" modified="2012-06-13" version="1">MEDDTTTAAAKDAVSMVLRLDGLLIEILLRVGFPTTLVRAAAVCKRWYHLASDMGFLRRFRKLNPPRLLGLYIHGSPVSSPITQRFVPVLPEPPELLAAAVRTVEGYSFPTRESEANLARHCRNGSVFTTLCGDLYGYQLQHEVHNPLCPRATAISINPRLTFNPAPPATGFEVLTREEEDGSLSYYYVFMELSYDEIGSTVPPKHWTARVYMLQDGIWRSHTTATPRIPVGRAGHSVLIQNKVYMTVTMRDISVLDLNTSSFSTVQLPDGVQHPASDFMLSCTPDGSSIYLVELKDFQLCILIYKGGCWSIADTILLVMCAGLMISDCTIEDAHSSSPWIKHVGDHAEFVLLQMGRHVRYLDTRCRTLRTLYEMSAEEQTWESIAIIPFMMIWPPVFPSLRNCNVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1J194</accession>
    <name>I1J194_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Oligopeptide transporter 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100835812</name>
        <name evidence="11" type="ORF">BRADI_5g20190v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter (TC 2.A.67.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581597.1">
        <property type="nucleotide sequence ID" value="XM_003581549.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J194"/>
    <dbReference type="FunCoup" id="I1J194">
        <property type="interactions" value="182"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ84335">
        <property type="protein sequence ID" value="KQJ84335"/>
        <property type="gene ID" value="BRADI_5g20190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835812"/>
    <dbReference type="Gramene" id="KQJ84335">
        <property type="protein sequence ID" value="KQJ84335"/>
        <property type="gene ID" value="BRADI_5g20190v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835812"/>
    <dbReference type="eggNOG" id="KOG2262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004965_0_1_1"/>
    <dbReference type="OMA" id="AMIWPAD"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="528"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="600"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="649"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="679"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ84335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="736" mass="82322" checksum="FB1D792018A1B0D9" modified="2012-06-13" version="1">MEIERTGGHGHPAAVDEDDDVSPVEQVRLTVPVTDDPTLPLWTFRMWTIGFVSCALLSFFNQFFAYRTEPIIISQITIQVAALPIGHFMARVLPERKLRVFGHECSLNPGPFNVKEHVLISIFANAGAAFGSGSAYAVGIVNIVKAFYKRNISFFTSLLLIITTQVLGYGWAGLLRKYVVEPAHMWWPQSLVQVSLMRAMHEKENRRMTRGKFFLIALICSFAWYTVPGYLFPTLTAISWVCWVFPSSITMQQIGSGMNGLGVGAVTLDWSMVASFLSSPLVSPFFATANVFAGYVLFVYIMLPVSYWVLNLYNASTYPLFSNDLFTGSGQLYNISAIVNDRFAIDLDAYGKQGKIHLSLFFAVSYGLGFATIAATLSHVVLFYGKEMYQRFRESYKGKTDVHTRLMRKYEDIPNWWFYLLLTVSMVLSMVLCTVFKEEVQLPWWGLLLACVVAFVFTLPISVITATTNTTPGLNIITEYCLGLIMPGKPIANVCFKVYGYMSMNQAVSFLTDFKLGHYMKIPPKSMFLVQFIGTLIASTVNMIVAWWLLTTVPHICEKDMLPEGSPWTCPGDHVFFDASVIWGLVGPRRIFGPLGYYNALNWFFLVGLFGPVVVWLAARAVPRHAGWISLVNMPVILGATANMPPASALNYTSWCLVGTVFNFFVFRYRKGWWKRYNYVLSAAMDAGVAIMGVLIFFALTSSGRQLDWWGSRGEHCDLAACPTAKGVVVDGCPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-04-02" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1J232</accession>
    <name>I1J232_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">At1g61320/AtMIF1 LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g22520v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
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                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="15368.I1J232"/>
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        <property type="gene ID" value="BRADI_5g22520v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_5g22520v3"/>
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            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ84740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="188" mass="20858" checksum="FF4D4E1EE5EC5525" modified="2012-06-13" version="1">MISTPTLPSKFVHLKYLRFTLHEDEAISPAYDYLSLVSFLEASPCLETFIFQVIQQGMKHDSIIGDSSHLRQLPEHRHDKLKSVAIGGFCSAKSLVELTCHIVENATSLEFLLLDTTQGSFSSDGCSVDKPGKCVAMGRDILLEACRARLAIRTHIEGIIPSRVKLDVVEPCSRCHDVQYIDKVSIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
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    </gene>
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
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        <property type="gene ID" value="BRADI_5g26760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104585469"/>
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        <property type="gene ID" value="BRADI_5g26760v3"/>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="48"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT62179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="195" mass="22841" checksum="82B282608E9F84AD" modified="2012-06-13" version="1">MGSVLTRLLDQPWGPYSSLPIDDDDICIRGPLTKKQQDAEYALFHAWTALKKYNSNHPGPDFCPVEAKAACVGFRQDFWYHVGFSARTIRRDDEQQQQQQQQERYYFFAELRFDRRSRGHLHVESCIMLEKPPGSYRSRCAFCPDRFKILHPSDAEFVCGKKGHRKKFFRERNMLGRPLCIRGFSQKHCFADVSV</sequence>
</entry>
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        <name evidence="1" type="ORF">BRADI_4g06936v3</name>
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    <organism evidence="1">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_4g06936v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ86654"/>
        <property type="gene ID" value="BRADI_4g06936v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EG11"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EG11">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ86654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ES67</accession>
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            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
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        <name evidence="3" type="ORF">BRADI_4g30910v3</name>
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ90342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003576511.4"/>
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    <dbReference type="STRING" id="15368.A0A0Q3ES67"/>
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        <property type="protein sequence ID" value="KQJ90342"/>
        <property type="gene ID" value="BRADI_4g30910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838629"/>
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        <property type="protein sequence ID" value="KQJ90342"/>
        <property type="gene ID" value="BRADI_4g30910v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838629"/>
    <dbReference type="OrthoDB" id="718560at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
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        <property type="expression patterns" value="baseline and differential"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="JAGGED/SL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45730:SF137">
        <property type="entry name" value="OS09G0430600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45730">
        <property type="entry name" value="ZINC FINGER PROTEIN JAGGED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ90342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="127" mass="13764" checksum="7AC010D84129E1F3" modified="2016-01-20" version="1">MMELNNSEQDEHEVNLELTLAPAAPPEPRGFFFCVYCDRKFRCSQALGGHQNGHKLERSLAKRRREIAAATRAHGAGAPSASDLLMPAAGKAAAAPVRGMARKRGRSLSEHGYGTIEGADEEDITFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F9D9</accession>
    <name>A0A0Q3F9D9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trichome birefringence-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_3g23055v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F9D9"/>
    <dbReference type="STRING" id="15368.A0A0Q3F9D9"/>
    <dbReference type="EnsemblPlants" id="KQJ96431">
        <property type="protein sequence ID" value="KQJ96431"/>
        <property type="gene ID" value="BRADI_3g23055v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96431">
        <property type="protein sequence ID" value="KQJ96431"/>
        <property type="gene ID" value="BRADI_3g23055v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3F9D9"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF53">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ96431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="150" mass="17447" checksum="8BB8A825820754CA" modified="2016-01-20" version="1">MVGKRLNVTTDIKEAFRLSLQTMTDWELSRARLSKSYFFFRSYSPSHYRNGTWDTGGSEEEYSWMNAMISSATRGLRTKGRNARFLNITYMTELRRDGHLSRHREPGTPPDAPEDCNHWCLPGVPDAWNQALYVHLLALGYDSRTKTEHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FCD9</accession>
    <name>A0A0Q3FCD9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100844092</name>
        <name evidence="16" type="ORF">BRADI_3g30230v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-malate + NAD(+) = pyruvate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:12653"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="12">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756963.1">
        <property type="nucleotide sequence ID" value="XM_014901477.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FCD9"/>
    <dbReference type="FunCoup" id="A0A0Q3FCD9">
        <property type="interactions" value="1103"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FCD9"/>
    <dbReference type="EnsemblPlants" id="KQJ97351">
        <property type="protein sequence ID" value="KQJ97351"/>
        <property type="gene ID" value="BRADI_3g30230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844092"/>
    <dbReference type="Gramene" id="KQJ97351">
        <property type="protein sequence ID" value="KQJ97351"/>
        <property type="gene ID" value="BRADI_3g30230v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844092"/>
    <dbReference type="OrthoDB" id="5365701at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FCD9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000005">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000237">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF73">
        <property type="entry name" value="NAD-DEPENDENT MALIC ENZYME 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="15">
        <location>
            <begin position="114"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="14">
        <location>
            <begin position="304"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQJ97351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="617" mass="68074" checksum="76FE282A741A660D" modified="2016-01-20" version="1">MWRHAAARRSAQIRRLLSSAAPPAVAGSAVRGPCIVHKRGTDILHDPWYNKDTAFPLTERDRLGLRGLLPPRVMSFEQQYERFINSYRSLEHNTKGEPESVVSLAKWRILNRLHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCENYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAEKVDMIVVTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQKVLPIMLDVGTNNEKLLENKLYLGLRQPRLEGEEYLAVVDEFMEAIHARWPKAIVQFEDFQMKWAFETLQRYRSRFCMFNDDVQGTAGVALAGLLGAVRAQGRPLTDFTKQKIVVVGAGSAGIGVLNMAKHAMLRMPGIHKIGELGEGHNQFWVLDKDGLITKARKGLDPAAARFARGFGPEEVQDLHEGASLVEVVRRVKPHVLLGLSGVGGIFNEEVLKAMKDSDSPCPAIFAMSNPTTKAECTPEDVFKYVGENAVFASGSPFSNVALSNGRKGYANQANNMYLFPGIGLGALLSGARHISDGMLHAAAECLASYITDDAIRKGILFPSISSIRHITARVGAAVARAAVDEDLAEGCCDMDPKELRSMSESDTVDYVARKMWYPVYSPLVNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FMD8</accession>
    <name>A0A0Q3FMD8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_3g48890v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FMD8"/>
    <dbReference type="FunCoup" id="A0A0Q3FMD8">
        <property type="interactions" value="825"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00367">
        <property type="protein sequence ID" value="KQK00367"/>
        <property type="gene ID" value="BRADI_3g48890v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00367">
        <property type="protein sequence ID" value="KQK00367"/>
        <property type="gene ID" value="BRADI_3g48890v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FMD8"/>
    <dbReference type="OrthoDB" id="29657at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FMD8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054103">
        <property type="entry name" value="CAND6-7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF23">
        <property type="entry name" value="OS02G0618700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21904">
        <property type="entry name" value="CAND6-7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999542" evidence="7">
        <location>
            <begin position="22"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="CAND6/7 N-terminal" evidence="9">
        <location>
            <begin position="24"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="8">
        <location>
            <begin position="200"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK00367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="467" mass="51422" checksum="7EFC0D773BEFED87" modified="2016-01-20" version="1">MGTISVAVAVVLVFLARAIHAEIRTMLIASDARPLILFEQFGFARGGKAALSIRRSEWSLRPGSRLAGGGVDPTLMGFVLISGAQFPKINNASEYAAADPAAADDGPGPGGKQHGARGFCLLTSGYAVPMIRLSDVPPGGATSILTIDDPDEYAVVFNNCQDGVEVTMDVRTEMYNVRPGGGDKAAGLLIRDYLPVGLQPLPTIYTVVSAVYLAFLAAWAWTCLRRQRATAERIHAVMGALLLFKALKMACAAEDMWYVERTGTPHGWDVAFYVFGFFKGVLLFTVIVLIGTGWSFLKPYLQDREKSVLMIVIPLQVIENLVLVVIGETGPTGRDWVVWNQVFLLVDVICCCAVFFPIIWSIRGLREASKTDGKAARNLQKLTLFKRFYIVVVGYLYFTRIIVSAFLAVLSYKYQWVVNVAIEAASLAFYLFVFYNFHPVEKNPYLYIADEQEESAGGQLEMDDRAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GTS0</accession>
    <name>A0A0Q3GTS0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DSBA-like thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g15070v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GTS0"/>
    <dbReference type="FunCoup" id="A0A0Q3GTS0">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GTS0"/>
    <dbReference type="EnsemblPlants" id="KQK14271">
        <property type="protein sequence ID" value="KQK14271"/>
        <property type="gene ID" value="BRADI_1g15070v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14271">
        <property type="protein sequence ID" value="KQK14271"/>
        <property type="gene ID" value="BRADI_1g15070v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GTS0"/>
    <dbReference type="OrthoDB" id="1930760at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GTS0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd03024">
        <property type="entry name" value="DsbA_FrnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887:SF41">
        <property type="entry name" value="THIOREDOXIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DSBA-like thioredoxin" evidence="1">
        <location>
            <begin position="11"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="217" mass="24418" checksum="88F432A9BF5969EB" modified="2016-01-20" version="1">MASDTGKKLIQIDVSSDTVCPWCFVGKKNLEKAMEQTKDKFDFEVRWHPFFLNPEAPKEGIRKSDFYKTKFGPVQFERATVRMTEMFRGLGFEYDMSGLTGNTMDSHRLIAFAGHQGYDKQNALVEELFLSYFCQGKYIGDRQVLLDAARKVGIEGAEELLQDPSKGVDEVKEELNKYSSGISGVPHFVINGKYQLSGGQPPNIFMRAFDAAAKDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H348</accession>
    <name>A0A0Q3H348_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100829423</name>
        <name evidence="5" type="ORF">BRADI_4g13857v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H348"/>
    <dbReference type="EnsemblPlants" id="KQJ87842">
        <property type="protein sequence ID" value="KQJ87842"/>
        <property type="gene ID" value="BRADI_4g13857v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87842">
        <property type="protein sequence ID" value="KQJ87842"/>
        <property type="gene ID" value="BRADI_4g13857v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="657261at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18280">
        <property type="entry name" value="BTB_POZ_BPM_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF483">
        <property type="entry name" value="OS11G0619800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="4">
        <location>
            <begin position="23"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="201"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ87842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="40142" checksum="6D5E11FC55423E2D" modified="2016-01-20" version="1">MSSSPLSAVLPTTASAVVTKAVAGSHVLKVKGYSLIKGLGVGKFIEFGKFSVGGSSWTVRFYPDGGPGSDYCADWVSIALFLLDPNPTTDVRANFKFNLLDQAQGKHVELNPQPGMRSFSNAKTGFGQDRFIKRMELDESTYLKDDCLEIRCDVTFALMKQISKVAVEAAKTQFVAVPPSDMHRHLAALLSGASGGGEGSVDVRFEVGGETFAAHRCLLAARSSVFSALLFGPMKEKTATCVKIEDMEPRVFKAMLHFIYTDSLPEMDKGETTVMAQHLLVAADMYNLPRLKLICEHELCKHVDGSTVDTMLALADQHGCRGLKEACLSTINFTRNLKETTASDGSEDSASAFEILAGREVTLRRGQAAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H6Q5</accession>
    <name>A0A0Q3H6Q5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100834370</name>
        <name evidence="14" type="ORF">BRADI_1g43717v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560820.1">
        <property type="nucleotide sequence ID" value="XM_003560772.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H6Q5"/>
    <dbReference type="STRING" id="15368.A0A0Q3H6Q5"/>
    <dbReference type="EnsemblPlants" id="KQK18631">
        <property type="protein sequence ID" value="KQK18631"/>
        <property type="gene ID" value="BRADI_1g43717v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834370"/>
    <dbReference type="Gramene" id="KQK18631">
        <property type="protein sequence ID" value="KQK18631"/>
        <property type="gene ID" value="BRADI_1g43717v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834370"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H6Q5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990538">
        <property type="term" value="F:xylan O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF64">
        <property type="entry name" value="OS06G0273700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="13">
        <location>
            <begin position="74"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="12">
        <location>
            <begin position="136"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK18631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="442" mass="48982" checksum="73DB702FAFF92CAE" modified="2016-01-20" version="1">MIPSLRGMLRGLLTRGTEGRRRRHAIHKTAPVPGAAVAIVFFATGTLCFVFLGPLRSPPTPTTTSSSGKAAATACDATRGEWVHDPSLRPHYTNATCGFIQDYQNCMKHGKPSLEFLQWKWRPDAADAGCGELMERFDAARFFRAVRGRSILFVGDSLASSHVLSLVCTLSELETPTKRHSTGFEQWWFEAHDFAVSFFWTPFQIRWRLTSDGPESTAPGPARQGEVFAGPSDLHLDEPDERWMSAAKEHDYVVLSASHWFRRPAVYYRGGHVVGCHGCSAKDADNVTGTELSPEHAQRVAFRTVLRALARGEHGFEGTAVLRTVAPTHYEKGGWFDGGECPSTRPLGRDRTAEMVEPEAGFYRAQVEEFRAAAKEARRSGGAVRLRLMDVTGMMLRRPDGHPGRYGHGPGQHDGFDVDCLHWCLPGPIDVWNELLLQILDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HDN7</accession>
    <name>A0A0Q3HDN7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g55775v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20629.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g55775v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g55775v3"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47746:SF75">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47746">
        <property type="entry name" value="ZF-RVT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK20629.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14410" checksum="BA02BB8B975257D2" modified="2018-03-28" version="2" fragment="single">WCPFCHNVIETAAHILLDCSFARAIWTRIAAQFNSPLIDPSSWSGVTSVRSWWIERSLASSTMGKPRAKATASLILLTLWETWKERNRRIFQHKLLRSSVVCALIKEEAALWIKAGAGIGELVSESTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I1F4</accession>
    <name>A0A0Q3I1F4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g44996v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99674.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I1F4"/>
    <dbReference type="EnsemblPlants" id="KQJ99674">
        <property type="protein sequence ID" value="KQJ99674"/>
        <property type="gene ID" value="BRADI_3g44996v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99674">
        <property type="protein sequence ID" value="KQJ99674"/>
        <property type="gene ID" value="BRADI_3g44996v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3I1F4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ99674.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="88" mass="10138" checksum="3B5176BAA33E0347" modified="2018-03-28" version="2">MQAGRHDRSSCPRMDCHQAMYTSRGIFHVGHGANWKLEVVLANTEVQLSGYLSRKTTTVRNRQLWHARSDTDTVEQTRTTDDGRRTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I3B0</accession>
    <name>A0A0Q3I3B0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC112271480</name>
        <name evidence="2" type="ORF">BRADI_3g14886v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95051.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316393.1">
        <property type="nucleotide sequence ID" value="XM_024460625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I3B0"/>
    <dbReference type="STRING" id="15368.A0A0Q3I3B0"/>
    <dbReference type="EnsemblPlants" id="KQJ95051">
        <property type="protein sequence ID" value="KQJ95051"/>
        <property type="gene ID" value="BRADI_3g14886v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112271480"/>
    <dbReference type="Gramene" id="KQJ95051">
        <property type="protein sequence ID" value="KQJ95051"/>
        <property type="gene ID" value="BRADI_3g14886v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="695450at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835:SF60">
        <property type="entry name" value="OS10G0490300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ95051.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="68434" checksum="EBB1E3AED6F79C5A" modified="2018-03-28" version="2">MELSSPSSLNTTAGGGGHDHHESESEDSAEENNPKCRVQFISDSSYEDDDHINELEHTDSDNQHTKFFHDVTDMVLQGLKRKRRRKSMRKESRKRAKFGIQLAGGNEAEIVPKAYSRCNVHYLHEVIRSFRNCERKCGLIRGAGFGDILQFDGCIVPRSFAQWIADCTNVRTEEVCVGEQRIQLGTEHVASIVGIPSAGLTIQTTGEDGKISFLASIGLKELPPIKFFGSKVMNEDLPDDMFIRCVLVVVLSTFLCPTSSTFPSTKYLGALVDIDKIKDMDWAKLTHNWMINCIKKYQKQRAKRKRLTCTLGGCIYSLAVRCLDEVDFEGIHLEQTLPRILVWKGDMIIQYSKLYMCPNGKYGFLPVKENNKSSFSIANKKESREKLLMELKNFLECSMTQSLSSEVKENVVSIFDAYMGTEDEHCWEKAKRLLTDVLMSITTNCNIMLKEEHHNGNIGILSGDTEVAGSSQEASYNCEGASDNTLKVDSDGASTNEGTTNGERKKEQPPHSGDKSKQDSMEHKDVTTTQFESGNKGGDGMHFAHTVQELDGTNGNVTGLSNPIMPTVMELDTYAKDVNKDVHLTDHQYEKTSKLPTTDTSAVQNEIALHVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I912</accession>
    <name>A0A0Q3I912_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100822959</name>
        <name evidence="6" type="synonym">MED17</name>
        <name evidence="7" type="ORF">BRADI_2g00620v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569428.2">
        <property type="nucleotide sequence ID" value="XM_003569380.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I912"/>
    <dbReference type="FunCoup" id="A0A0Q3I912">
        <property type="interactions" value="1434"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3I912"/>
    <dbReference type="EnsemblPlants" id="KQK02291">
        <property type="protein sequence ID" value="KQK02291"/>
        <property type="gene ID" value="BRADI_2g00620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822959"/>
    <dbReference type="Gramene" id="KQK02291">
        <property type="protein sequence ID" value="KQK02291"/>
        <property type="gene ID" value="BRADI_2g00620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822959"/>
    <dbReference type="OrthoDB" id="2020583at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3I912">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019313">
        <property type="entry name" value="Mediator_Med17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114:SF7">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10156">
        <property type="entry name" value="Med17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK02291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="659" mass="73830" checksum="24253E3DF71FED6E" modified="2016-01-20" version="1">MAGMDEGVRLDLDKLPIKRLDAIDEAGNEHYPPDTSSEEQRLAAIRRVDFSWVIERDAKKAKKAAEDTAQQSWQWQGLMESLQQAQQELTVVLDLISTVEANDAVAVTTTSKPKSLPNEVLDDMAVSAATKLQRLRHLGRYFKQSAKTMEQQFQKEARFYGSLIRLQQNWKVKRHRFGATGPGSDSFMFDVVDTSQLDTAAMPRMSSMSLVPIDQDSSGTLSVQVPQKSCRFLSLQLRGDNASSAESYACKMKGISSTTSAAENDALENDDVNKSVKHAHSILRNIHKSIFEEQVFDMVIRETFVPSQGINVTGMREDFLQLAIGQENLLCLSLEHSGLDTDSEMEGLEEPTDSEDASNLVLATINGKQEPSKMDASGLNPKSLEIYLLHMFHENILRKVREKYRNIVRYPSPAQAAGDDCNLLGHFCMTVAHRIFSNKVHLELESVVSRVPYLHLQSLPTWHSRTSSWSLCLRVPQPILAADRVTKPLDNDEPKYKSRSQFNTKVILKDGQISLMGEGSPSIAGSLTRKPSDGYLINSYNCDLEDLPTMLLQQVASQVINWLHEEALVLGMNVTRDFLCLYFDLDQGDTLGLVAHVDPDDAYGCISWYLTIDHSAKDGKMSTDNPEFEKRRFLGYLSLEVLYSTLMDLINLCSTGVHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IBW2</accession>
    <name>A0A0Q3IBW2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hydrophobic seed protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104583695</name>
        <name evidence="3" type="ORF">BRADI_3g32950v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756205.1">
        <property type="nucleotide sequence ID" value="XM_014900719.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IBW2"/>
    <dbReference type="FunCoup" id="A0A0Q3IBW2">
        <property type="interactions" value="1399"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3IBW2"/>
    <dbReference type="EnsemblPlants" id="KQJ97739">
        <property type="protein sequence ID" value="KQJ97739"/>
        <property type="gene ID" value="BRADI_3g32950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583695"/>
    <dbReference type="Gramene" id="KQJ97739">
        <property type="protein sequence ID" value="KQJ97739"/>
        <property type="gene ID" value="BRADI_3g32950v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583695"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd01958">
        <property type="entry name" value="HPS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027923">
        <property type="entry name" value="Hydrophob_seed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051636">
        <property type="entry name" value="Plant_LTP/defense-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31731">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14547">
        <property type="entry name" value="Hydrophob_seed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129289" description="Hydrophobic seed protein domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrophobic seed protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ97739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="118" mass="12250" checksum="7D99F688A8019C1D" modified="2016-01-20" version="1">MAMAPRAVLLLAVSLLAMASAASALTCPKDGLKLKACVDVLGALKLRVNVPQDKACCSLLDGLAGVDAALCLCSRLTADVLGGLGLGLVNLDLPVNLRLLLNNCGMKCPDNFRCPHRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IIP9</accession>
    <name>A0A0Q3IIP9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g03166v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IIP9"/>
    <dbReference type="EnsemblPlants" id="KQJ86080">
        <property type="protein sequence ID" value="KQJ86080"/>
        <property type="gene ID" value="BRADI_4g03166v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ86080">
        <property type="protein sequence ID" value="KQJ86080"/>
        <property type="gene ID" value="BRADI_4g03166v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IIP9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ86080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="227" mass="24722" checksum="94E4B2A1707B7376" modified="2016-01-20" version="1">MAGQHTAALLCSAAEDMEGDAELPRPRRWPCGLLDLARPRTAAIGVIFPVSGPICFLTDSRTSLPEPRALSRRQEPKSHPPVSLAARRAPASDVLRPRCSSRRSPVSPTAAGPRDAPLCDRRPRQPLQRTASPSLVDSGRATSPPPPCAALARRPASLTPSCCVRRPLRSPSSPPSQPVAPSCALLVREEEKRGEKKREGMEGENGWRRERRGESMTGRLEKKIEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JDG2</accession>
    <name>A0A0Q3JDG2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g22605v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JDG2"/>
    <dbReference type="EnsemblPlants" id="KQJ96352">
        <property type="protein sequence ID" value="KQJ96352"/>
        <property type="gene ID" value="BRADI_3g22605v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96352">
        <property type="protein sequence ID" value="KQJ96352"/>
        <property type="gene ID" value="BRADI_3g22605v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JDG2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ96352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="95" mass="10198" checksum="0C8B675721FA772A" modified="2016-01-20" version="1">MTELKTETSGEKCPFTSRVQPRAPLGKPQIAIVATPLTPMAAPQHAREATRPPRLETRRNSTAAAVRRGSDTPPNGPPPPRRANAAPLGIAHHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K647</accession>
    <name>A0A0Q3K647_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g25475v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K647"/>
    <dbReference type="EnsemblPlants" id="KQK06281">
        <property type="protein sequence ID" value="KQK06281"/>
        <property type="gene ID" value="BRADI_2g25475v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06281">
        <property type="protein sequence ID" value="KQK06281"/>
        <property type="gene ID" value="BRADI_2g25475v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K647"/>
    <dbReference type="OrthoDB" id="692410at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK06281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14495" checksum="072B5C03DF0BBBF5" modified="2016-01-20" version="1">MKQQILTSDECPFGCNSSESALHLAVLCPRSALIFQYLGLDVSNVQDMRDLFTDGKVVIAPNKKNVWVVVLVAILWNIWLARNRKVFDDRNIPHIVVARQCTETLKLWSSRLKKNERQAADQWIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LCC6</accession>
    <name>A0A0Q3LCC6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytokinin hydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100829850</name>
        <name evidence="12" type="ORF">BRADI_4g29860v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578093.1">
        <property type="nucleotide sequence ID" value="XM_003578045.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LCC6"/>
    <dbReference type="STRING" id="15368.A0A0Q3LCC6"/>
    <dbReference type="EnsemblPlants" id="KQJ90173">
        <property type="protein sequence ID" value="KQJ90173"/>
        <property type="gene ID" value="BRADI_4g29860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829850"/>
    <dbReference type="Gramene" id="KQJ90173">
        <property type="protein sequence ID" value="KQJ90173"/>
        <property type="gene ID" value="BRADI_4g29860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829850"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LCC6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF228">
        <property type="entry name" value="CYTOKININ HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ90173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="59665" checksum="0E366CF69551453E" modified="2016-01-20" version="1">MGFVEAMAMATASATLALLLGAAWVTLSCYLLTPMRIRRAMAAQGVHGPPPRPLVGNLREVSALVAEATAGDMPSLSHDIVGRLMPHYVLWSRTYGKLFVYWYGSEPRLCLTDTGMIKEFLSSKYAHATGKSWLQRQGTKHFIGRGLLMANGARWSHQRHVVAPAFMPDKLKGRVGHMVECTKQTMVSLRDAAARGRGEVEIGAHMTRLAGDIISRTEFDTSYETGKRIFHLLEDLQRLTARSGRYLWIPGSQYFPSKYRREIGRLNGELECVVMESIRRSGEIADEGRTTSTYGRGLLAMLLAEMEKKKEKAGSTADDGKFSYDTRLVIDECKTFFFAGHETSALLLTWTLMLLATHPEWQDKARAEVATVCGDQPPSADHLPKLTVLQMIIHETLRLYPPATLLPRMAFEDMTLGEGRLRLPRGLSVWIPVLAIHHDEFIWGADAHEFRPERFAPGARRGSSSSGAARFLPFAAGPRNCVGQTYALFEAKVVLAMLLAEFRFAISDDYRHAPVNVLTLRPKHGVPVHLRPLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3MBR8</accession>
    <name>A0A0Q3MBR8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YbaK/aminoacyl-tRNA synthetase-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100831042</name>
        <name evidence="4" type="ORF">BRADI_3g57868v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRORSD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570559.1">
        <property type="nucleotide sequence ID" value="XM_003570511.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3MBR8"/>
    <dbReference type="EnsemblPlants" id="KQK01734">
        <property type="protein sequence ID" value="KQK01734"/>
        <property type="gene ID" value="BRADI_3g57868v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831042"/>
    <dbReference type="Gramene" id="KQK01734">
        <property type="protein sequence ID" value="KQK01734"/>
        <property type="gene ID" value="BRADI_3g57868v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831042"/>
    <dbReference type="OrthoDB" id="424586at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3MBR8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04335">
        <property type="entry name" value="PrdX_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.960.10:FF:000005">
        <property type="entry name" value="Putative prolyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="entry name" value="YbaK/aminoacyl-tRNA synthetase-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040285">
        <property type="entry name" value="ProX/PRXD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31423:SF3">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE ASSOCIATED DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31423">
        <property type="entry name" value="YBAK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="YbaK/ProRS associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YbaK/aminoacyl-tRNA synthetase-associated" evidence="3">
        <location>
            <begin position="24"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK01734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="320" mass="34637" checksum="2FCA89AF469FFEEF" modified="2016-01-20" version="1">MGYSKDELLARLEELKIEFTCYGHPAVLTVEEQAKHVGNFGGALSKNLLLKDKKHRLYIVSALADTKVDMKILSQRLGLGKGGLRMAPEENLQQVLQVPLGCVTPFALFNESASAVSLLLDQGFKSKTSCYFHPLTNDVTIALNSSNLDKFLISIGKEPAYVDLEASPAVGKDNPPDLADFVPSGALNSSEPVENMTPTPAPQRNNVPKEKTPEVKAKPKVQNKGAEKPESKVSANGTNVDKFVNDVFDIISPLFLSEVSKKLNIKPEELSPIVDDLRGRAAPDLESLTGILKGASYTAGFHAGFETFRDFGLSGIPSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NM20</accession>
    <name>A0A0Q3NM20_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g43152v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18526.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NM20"/>
    <dbReference type="EnsemblPlants" id="KQK18526">
        <property type="protein sequence ID" value="KQK18526"/>
        <property type="gene ID" value="BRADI_1g43152v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18526">
        <property type="protein sequence ID" value="KQK18526"/>
        <property type="gene ID" value="BRADI_1g43152v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NM20"/>
    <dbReference type="OrthoDB" id="686875at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039266">
        <property type="entry name" value="EN-1/SPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157">
        <property type="entry name" value="AUTONOMOUS TRANSPOSABLE ELEMENT EN-1 MOSAIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33157:SF12">
        <property type="entry name" value="TRANSPOSASE TNP1_EN_SPM-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="125"/>
        </location>
    </feature>
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        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK18526.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="369" mass="41596" checksum="2F23395F6B37AF12" modified="2018-03-28" version="2">MVGGRSTSQRRVEELYPPPKVKANPPKKTVARKSKGKARREPSPLPPPLDPLYDPEDDREEEIQEEVREMEMEMEAGDFGEHELQDHSVGTSAGASAGAASSSGTDKVYQRGPTRLPTKQPSCEAEKPPVLGVLLKEHFPGMVLLEGKEEPEVARTWANHVAQPDFQDRENLVYANKAERVKGEFWRFFKLAEGIYDATIARHFKASVKKALSEMFYHTRAYSVRKYYAEVKKQKLDKAQCIVTHLKHEQYMQVPPHCPEYVETYGLLRERRLANPSGMHHQASVNLHGYINNYAKAHPDKERLTSFTAVDYTLTGKASTVSEYTYATPTDHAKVTGYAEVFEEVHGPGADPSQHDLDVEVVVRTCCRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NUN0</accession>
    <name>A0A0Q3NUN0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g59130v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NUN0"/>
    <dbReference type="FunCoup" id="A0A0Q3NUN0">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3NUN0"/>
    <dbReference type="EnsemblPlants" id="KQK21174">
        <property type="protein sequence ID" value="KQK21174"/>
        <property type="gene ID" value="BRADI_1g59130v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21174">
        <property type="protein sequence ID" value="KQK21174"/>
        <property type="gene ID" value="BRADI_1g59130v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NUN0"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3NUN0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF57">
        <property type="entry name" value="OS07G0112400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="4">
        <location>
            <begin position="94"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="225"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="322"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="395"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="234"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK21174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="615" mass="65849" checksum="A09430AFC273BEDA" modified="2016-01-20" version="1">MDYGGGRTGPATLAATRLRRSTESHDAYTVRIAYRRLVALATATPADARRWICTTRWLHASLLRSGRLVIGLGVQWTPVRGPLPPNHTPPPATLQLCAGHRCLVFHLAQLDSPIPAALYRFLADPRVLCVGYGSAYDRRMLLDHFGLRVASGRDLRALAGMGGNVSVEEMAARFLGFVGVRKPREVAMSAWHRPRLDAAQVEYAAADAYLAFRLGVLLCSGEAAGHHHHHHQQPAPPAPVIRRAPPPAAAAAPRAPPRIVHEPLPPGPRVAVLNRAPAPAPAQVPPRAPVVRRGPQLIVHAPLPPGPRVAELRRAPAPAQVLPRAPPRAPVVHHAPAPAQARPRATPTAPRAVFVPRAPAPAPRALVIPCPPPVTRAPEQQPALAPTAVVVDAADGSSSKTAETHPAGVTGSLTTGGGGLPMIRSSYASSDSDDGLPMIRSSYSPGSEDSDFCSDDFELLGREAVTNEDEEEQQHISDYLAGFLSDGEYVVDTGVPIDDNEEDGVVEEYTGMGVLTVRDHIAEGYKEYTGILTVRNDTDTTYNDNDDEAFVVGSEEPEYYSVVGGADCYGNSTAEAFNGGEDDGYYCQDGGSVWYDQGDDGDRYVQDDDSYDAFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PKI4</accession>
    <name>A0A0Q3PKI4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BRADI_4g28292v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQJ89881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PKI4"/>
    <dbReference type="STRING" id="15368.A0A0Q3PKI4"/>
    <dbReference type="EnsemblPlants" id="KQJ89881">
        <property type="protein sequence ID" value="KQJ89881"/>
        <property type="gene ID" value="BRADI_4g28292v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT64412">
        <property type="protein sequence ID" value="PNT64412"/>
        <property type="gene ID" value="BRADI_4g28292v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89881">
        <property type="protein sequence ID" value="KQJ89881"/>
        <property type="gene ID" value="BRADI_4g28292v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64412">
        <property type="protein sequence ID" value="PNT64412"/>
        <property type="gene ID" value="BRADI_4g28292v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840345"/>
    <dbReference type="OrthoDB" id="672805at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000168">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5006206498" description="Protein kinase domain-containing protein" evidence="19">
        <location>
            <begin position="29"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="353"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="652"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="652"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="705"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ89881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="718" mass="78495" checksum="6BF4679D3DF919F7" modified="2016-01-20" version="1">MEMKTSSCLLGLSFPCLLLLVIFPCATSLSFNYNFSGADAGVLTDAGILKYMEDSAAATDRIELTNTSRSWSTGRVAHMQPVRLWEDKKYVASFTSSFTFAIVESSDGRPRGDGMAFFVAPYTTSPREMPVEMPQDARGGFLALFNNPNNSANTLPRTVAVELDTNRNDGWDPPSPIIDHIGIDVNDIRSIKYENLTSGSLNGIMSALVRYDAKAATLSATLWFVDPPRQGPVTVSANVDLREAGLPQDAAVGFSAATGNSSELHQILAWSFESTMTDTTTKNIGLVAGLVSGGVFILLAIAAWVGYLQYHKRKGMQLEDAEIHLDQDMDEEFEMCGPRKFSYTELSQATQGFSEKEKLGGGGFGAVYRGFLQEQVLHVAIKKVSETSRQGRREYIAEVTIIGRLRHRNLVNLVGWCHKSDEFLLVYELMENRSLDVHLYNSKQVLAWPTRYKIILGMGSALFYLHQECEQCVLHRDIKPSNVMLDSSFNAKLGDFGLARLVDHSRGGFTTAQVAGTRGYMDPEYVYSGRATTESDVYSFGVVLLEIACGRRPVIEHRDESKVVLVDWVWALHGRKMLLDAVDARLDGGFDAQEMERVLVVGLWCVHPDYGFRPSIRQALNVLRCEAQLPDLPPEMPVATYARPHAVHGWSSYMSSSTGAGSSATTGGRSSKSDQMIGSAKSHSFATAADTGGATRPSAAAADQNDATSSTGEHSGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RD95</accession>
    <name>A0A0Q3RD95_BRADI</name>
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        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100841629</name>
        <name evidence="4" type="ORF">BRADI_2g58500v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK11162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3RD95"/>
    <dbReference type="EnsemblPlants" id="KQK11162">
        <property type="protein sequence ID" value="KQK11162"/>
        <property type="gene ID" value="BRADI_2g58500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841629"/>
    <dbReference type="Gramene" id="KQK11162">
        <property type="protein sequence ID" value="KQK11162"/>
        <property type="gene ID" value="BRADI_2g58500v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841629"/>
    <dbReference type="OrthoDB" id="767661at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RD95">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051240">
        <property type="entry name" value="Mito_RNA-Proc/Resp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933:SF32">
        <property type="entry name" value="OS06G0152500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="329"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="401"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK11162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="54827" checksum="BB2A15129120D557" modified="2016-01-20" version="1">MGGGATPVRWPRVLAPAHLAGAIRRQKNPLEAARLYDDAPRWYARSSYRHSDTVHASLLGAASASPSLLPSLLRRILRSSPSADALLASSIPHLPPEDAVTIFRSSLPSSPAPSWSLSFSALLRRLLSRGLLPEAARLLADFQGRPEVSVSSEDISLLISEMCRVRRPDLALQVLEEMPGQCLAPDRGAYHTIVPALCNAGMLEEATHVVYSMLWRVSQRGCDVDVVVYRALLVALCAAGQGEQAEIVLDKIIRKGLRTPGSRRSLRVPMLGGLSIDDAREAIDRALVVRGGRTVASFESMVLDLYDEGCLNEADNLCKDMGKKGFKPTICMYEAKIIALCREQRVDDAIKVLEEELAKSGLVPTVTTYNLLMKGLCDIMQSMKALGFLKRMDKQLGCVARKETFSILIKGLCSESQFVEAARVMERMVKGHRRPERTEFSNVIEGLCYLGRTYDALLWLEEMIDHGETPDVRVWSSLVSSALGLGESVVVAAACELVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S744</accession>
    <name>A0A0Q3S744_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g57575v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3S744"/>
    <dbReference type="EnsemblPlants" id="KQK20922">
        <property type="protein sequence ID" value="KQK20922"/>
        <property type="gene ID" value="BRADI_1g57575v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20922">
        <property type="protein sequence ID" value="KQK20922"/>
        <property type="gene ID" value="BRADI_1g57575v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3S744"/>
    <dbReference type="OrthoDB" id="43654at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="1">
        <location>
            <begin position="10"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK20922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="127" mass="13516" checksum="0FC270507BB4711D" modified="2016-01-20" version="1">MENYCSSKPGTLQAILAAHDAARAEVGVASLKWDAAAAAYAQNYANERRADCRPVHSGGPYGENIFVGGPRESAVAAWVAQKADFDRAGNTCLNGRPCGHDTQVVWRGLFTVCSYSPRGNILGLSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CEW4</accession>
    <name>A0A2K2CEW4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g01744v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CEW4"/>
    <dbReference type="EnsemblPlants" id="PNT60569">
        <property type="protein sequence ID" value="PNT60569"/>
        <property type="gene ID" value="BRADI_5g01744v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60569">
        <property type="protein sequence ID" value="PNT60569"/>
        <property type="gene ID" value="BRADI_5g01744v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CEW4"/>
    <dbReference type="OrthoDB" id="1921280at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CEW4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35992">
        <property type="entry name" value="CYTOMATRIX PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35992:SF6">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="131"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT60569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="184" mass="20981" checksum="384826BD160BE373" modified="2018-03-28" version="1">MGRGGGAGPARGDGGSWLRTYDRVVAMVGKVEALVAGRAQLEAVNELQHEFWEDRGGVLQARFLQAEESRKKWEAAYMDLLPHDDHRKFAELQESDLEDFTTFIDLLTAENSELKIQLKEVRSCVEDKKNTTDHEQTARSLRAELRKLKEAHETLSSKKDKEVAALIAEKDLVQNQLRRKKEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGA8</accession>
    <name>A0A2K2CGA8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_5g09871v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CGA8"/>
    <dbReference type="EnsemblPlants" id="PNT61063">
        <property type="protein sequence ID" value="PNT61063"/>
        <property type="gene ID" value="BRADI_5g09871v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61063">
        <property type="protein sequence ID" value="PNT61063"/>
        <property type="gene ID" value="BRADI_5g09871v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CGA8"/>
    <dbReference type="OrthoDB" id="657261at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CGA8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18280">
        <property type="entry name" value="BTB_POZ_BPM_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF483">
        <property type="entry name" value="OS11G0619800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="4">
        <location>
            <begin position="24"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="188"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT61063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="39528" checksum="9A3DFB54A07F73CD" modified="2018-03-28" version="1">MLAGCDSDASAQHTASAIVAKTVSGSHVLKIKSYSHTKGLGLGGILSDTFRVGGHCCRIVYYPDGCRSDCADWVSIGLLFVETHASHINARIKFSLLDHGGKPVPLYTTETHTVTTLSTRQEPLVSARFIERKTLESSYLKGDCFTVRCDVTITSGIRTEDTTKQSVVVPLSDIHQHLGQLLLTGKGTDVTFEVGRETFAAHRCVLAARSPVFMAELFGPMKENTATSIRIDDLEPSVFQAFLYFVYNDSLPDIDKGEEILMAQHLLVAADRYDLKRLKLICEDILCNSIDTETAAATLALAEQHGCCGLKDECFKFLTTPGKNLKLVMTSDNFQHLWSVSPSVLKELLAKLDAINGLQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CIP7</accession>
    <name>A0A2K2CIP7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g22737v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CIP7"/>
    <dbReference type="EnsemblPlants" id="PNT61903">
        <property type="protein sequence ID" value="PNT61903"/>
        <property type="gene ID" value="BRADI_5g22737v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61903">
        <property type="protein sequence ID" value="PNT61903"/>
        <property type="gene ID" value="BRADI_5g22737v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CIP7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT61903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="172" mass="19766" checksum="0C28B5073733304F" modified="2018-03-28" version="1">MLYTDEPPLPHAWVGCSDAAIIFSVKATKLDDDLEWPCGISSILRNRNLLFNRRPQTTVRRSQRRVMLGHCWIIKHGFAVAVSIGYFTQVQEPRSRLWTLLLAGPILRGEAAELSARCSPRFEGFLDDADLWYEDLHKFLALNCGRSDRGYVWLLDGLESVQFFLCLDHERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CJI5</accession>
    <name>A0A2K2CJI5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106865586</name>
        <name evidence="2" type="ORF">BRADI_5g26941v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT62192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CJI5"/>
    <dbReference type="EnsemblPlants" id="PNT62192">
        <property type="protein sequence ID" value="PNT62192"/>
        <property type="gene ID" value="BRADI_5g26941v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62192">
        <property type="protein sequence ID" value="PNT62192"/>
        <property type="gene ID" value="BRADI_5g26941v3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CJI5">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT62192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="207" mass="23400" checksum="14BEB11B00D600AD" modified="2018-03-28" version="1">MAALLGRRLRGSALQPTPVGLHFLPRLSHAHAHAQHSRVGGGAKPAIESRRNQKTPSRKARRRSMAALVGRRLGGSVLQPTQATTRLLFFPGLIHSQIHIMDDSPASQIQQKKDELYDLLDKQKRLTCQNGRLMHNLSVQINPRPDDLRWHGFRIRKRLDYVVDAVMHSTIFILLFGAWLSWNEESSAEDGKEEALGVKNTQEQGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CL79</accession>
    <name>A0A2K2CL79_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g08195v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CL79"/>
    <dbReference type="EnsemblPlants" id="PNT62787">
        <property type="protein sequence ID" value="PNT62787"/>
        <property type="gene ID" value="BRADI_4g08195v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62787">
        <property type="protein sequence ID" value="PNT62787"/>
        <property type="gene ID" value="BRADI_4g08195v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CL79"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT62787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="10671" checksum="A03FB1828CA7B879" modified="2018-03-28" version="1">MMLTCYFDHVIYFLKSCYLRQTRINLYIVFITCYGDQIIASYLMCRIIEHGKAFKVKPFILLLPSKYLIFCNIIYILLKIFLSLCFYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CN86</accession>
    <name>A0A2K2CN86_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_4g16674v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CN86"/>
    <dbReference type="EnsemblPlants" id="PNT63498">
        <property type="protein sequence ID" value="PNT63498"/>
        <property type="gene ID" value="BRADI_4g16674v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63498">
        <property type="protein sequence ID" value="PNT63498"/>
        <property type="gene ID" value="BRADI_4g16674v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CN86"/>
    <dbReference type="OrthoDB" id="779507at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
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        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
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        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
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        <property type="entry name" value="OS01G0216300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT63498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="38287" checksum="43C31F062A2301E6" modified="2018-03-28" version="1">MDPLRGKRALPEARNLLLCRGFSDSYSDTGNFVELAGGILPVVPFSNLPYGETFFGQPTGRASNGRLIVDFVGRNFTGGANFAVVGATALDQAFFLKEKIAVPPFNSSLDVQLEWFQKLKPTLCSTKRGCKDCFRRSLFFMGEFGGNYTFIMAAGKTVLQAMSYIPDVVRAISVGVEALLEEGDRYVVVPGTPPGGCLPIILTLYARQDEAKYDMGTGCLKAHNTLAAYHNAAVLTYPKAKVIYADYYKPVMAFLREPKIYGFNASSRLRECCGAGGPYNYDVTAACGFPTASACENPAAALNWDGVHLTETAYERIASGWLQGPYAHPPILTATDMPGLLTSDNTDEAHDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CNJ3</accession>
    <name>A0A2K2CNJ3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g18203v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CNJ3"/>
    <dbReference type="FunCoup" id="A0A2K2CNJ3">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CNJ3"/>
    <dbReference type="EnsemblPlants" id="PNT63600">
        <property type="protein sequence ID" value="PNT63600"/>
        <property type="gene ID" value="BRADI_4g18203v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63600">
        <property type="protein sequence ID" value="PNT63600"/>
        <property type="gene ID" value="BRADI_4g18203v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CNJ3"/>
    <dbReference type="OrthoDB" id="604989at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000767">
        <property type="term" value="P:positive regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Znf_CCHC"/>
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        <property type="entry name" value="Znf_CCHC_NABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23002">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="230"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="259" mass="28697" checksum="20CDD3F123518F18" modified="2018-03-28" version="1">MSGIVTCLVCGKEGHYTCDCPMKDQEGKQNMSDNRACTRCGEIGHSASTHGLDCSSCDKYHPLGECRMSKVTCLMCESRDHFLAQCPLNSVLTTVLQDQRENFRGALLLALTKQVTSDNTPTRVFGRLCLNCHEEGHYAKHCPLKLQDVSQSSLSGESNTRAISGDLSVELKEHGPSTAKHSSKINLTSSYECFNCGVEGHRVWNCPLKQQLLTPNKSRPLVKAGKTRVCYSCGEVGHYAQQCPLKRQKCQDYRTKSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CSL9</accession>
    <name>A0A2K2CSL9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g36106v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CSL9"/>
    <dbReference type="EnsemblPlants" id="PNT65012">
        <property type="protein sequence ID" value="PNT65012"/>
        <property type="gene ID" value="BRADI_4g36106v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65012">
        <property type="protein sequence ID" value="PNT65012"/>
        <property type="gene ID" value="BRADI_4g36106v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CSL9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036043260" evidence="1">
        <location>
            <begin position="22"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT65012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="54" mass="6158" checksum="5F5D4CE86A2FBE04" modified="2018-03-28" version="1">MRKRVWQLALLFSMALRLGDPAHSLDESLCLHCCNTCSLLIGWELEPVPRPTVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CU24</accession>
    <name>A0A2K2CU24_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein PRD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100832689</name>
        <name evidence="2" type="ORF">BRADI_4g44050v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757920.1">
        <property type="nucleotide sequence ID" value="XM_014902434.2"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CU24"/>
    <dbReference type="EnsemblPlants" id="PNT65531">
        <property type="protein sequence ID" value="PNT65531"/>
        <property type="gene ID" value="BRADI_4g44050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832689"/>
    <dbReference type="Gramene" id="PNT65531">
        <property type="protein sequence ID" value="PNT65531"/>
        <property type="gene ID" value="BRADI_4g44050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832689"/>
    <dbReference type="OrthoDB" id="2019943at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CU24">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044968">
        <property type="entry name" value="PRD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36379">
        <property type="entry name" value="PROTEIN PRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36379:SF1">
        <property type="entry name" value="PUTATIVE RECOMBINATION INITIATION DEFECT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT65531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1290" mass="141379" checksum="5E9B341D75A250E1" modified="2018-03-28" version="1">MESDGEVSPPSPPQAQGPCGAGHRASHSLPTSAGGRVCVSCAAALLSSASAPSHHVSHVLASLSLALADPAFLAPLRAAHPHLLAAPLADALAGAASRRDAALAAQASDLAADLASAVGPPAASGLVARLARLLSSGSLVKHLHTLHCMGVLLNSIEGAASCIGDKISLFLNLVNDLRLPSDEIRGEILFVLYKLSLLNSTPWDNICDNDNVDLSAIGRSLLQLSLEVLLKTQINAVRLNCIALLLTLAKKGAFDILLLGDQNLMNSIEAEGSRQTDDVSLNASIIVLFADAVKGSLLSTNLEVQTGTLDLIFHFLSSDANIFVLLQTLIDENVADYVFEVLRLSGSNDKLVISSIQVLSLLATSEERFKEKLAIGFSTLLPVFHYVAEIPFHPVQPQVLRLVWICIVNCSGILSLSQEEQIACTLTSVLRRNGNGELGMCSETFVLVCLILIEILRSPSAHNIQALPSFIEEASKHAISSTLPHGYDCAFLVPHSLRLLKEALIFCLEGNKENISVKKDLEDSVINICGTNLLQWLESAVADGDDDEALGEILQIFHIVLSRTSHNKQLKFAEMLASSSWFSLSFGFMGLFPTEDVKSVVYLVTSSVVDKILGCKYGETIRDAYVYLPSDPAELMHLLGQCSLEDFNLAACQCAILVILYACSFYNERLVGDNQILALVEQYILLNGGKFPYEITGHVMLTLLVHLYAFVRGISFSCSISHSPEVEKTLFRVMACNEWDFLLIRVHPIALKWLFQKGELMEPLSFQMLNFCRTFSEDKTVMLSNSSQLVDIQMIAELVLVGETSISYLLVSLLNQVVNEGTEDDVFSVVNVIHEILVIAPCSSDQFTSCGVVDAVGSIYSSPYSSRIKSLCSFLIFNILCSASVMALAQEDEWLALTMKLLEYFNSSLDCISSHQKHKILIGTFCLILHHSANKVLMEPAKAIILNSSLVSLTDGVIQEARAKGPSLLQHNQETDFGGFMIFILQLVYFSLRSLHAILDPNIDWQEFLQDSDNPQSFCVVGISCHDLCRLMYFGPCPIKLIASQCLLELITSISEQRSYLNAELRCSAKYMKSIIAVVEGLVFSQDSRVAENCGSSLSMILRWENFGSQENTLVRKSKWSRLIMEEFTVALTAPGLTSKSFSNHQKIAASIAVSLLKLSPVPDWLTSLFDNSLISGVVANLSARNITAEIVNLFSELMATKYLTQEHIVALHNLFQVCRRQVYEGSSKACLSEKKAEERVARSVDDERALLFDMVLNQHAASCTVQMEQQKLLHEIDLFFQESSQRQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYK7</accession>
    <name>A0A2K2CYK7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100840015</name>
        <name evidence="2" type="ORF">BRADI_3g21175v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756203.1">
        <property type="nucleotide sequence ID" value="XM_014900717.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CYK7"/>
    <dbReference type="STRING" id="15368.A0A2K2CYK7"/>
    <dbReference type="EnsemblPlants" id="PNT67112">
        <property type="protein sequence ID" value="PNT67112"/>
        <property type="gene ID" value="BRADI_3g21175v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840015"/>
    <dbReference type="Gramene" id="PNT67112">
        <property type="protein sequence ID" value="PNT67112"/>
        <property type="gene ID" value="BRADI_3g21175v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840015"/>
    <dbReference type="OrthoDB" id="674730at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056594">
        <property type="entry name" value="AT5G49610-like_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133:SF266">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133">
        <property type="entry name" value="OS07G0120400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23635">
        <property type="entry name" value="Beta-prop_AT5G49610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="1">
        <location>
            <begin position="397"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="454" mass="50429" checksum="E7CC22D74D462311" modified="2018-03-28" version="1">MELPYFSDDVFYEIILRLPPRPSTLLRASLVCKRWRGIVSDPQFLPKFVIHHRSPPLLGFFSGYFGKPAFTAALDPPDRIPAELFSMPRQLYESFSIPPRLNERWSLVGCRHGLVLFLHQAPLAMVWDPITGANTCIAVPLEFATADRIICHGAVLCAAAAGGAHVHCGLRTCPFKVVVIFIDDHYTQAFASVYSSESGKWGRVFSTPIPSPVSPRQPSTLVGNALYWMLINNGILELHLDRQCLAVIENPVGGPLLTDHFGFQITASPDGELGLASLARSIIQLWERKTNSGGVPTWEWGETIDLDMILALESGKDRIRLGILGFSEDANAILVSMGTDAFLVHLDSKQVRKLATSVSVTAYHPYTSFAIPGCDAKRLIHADKGGDLLMVPTDLFLSGWHYLDPRGDEQGPFSMVELRQWKRSGYFSQDFRVWRAGQTREQAILLTDAMLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D5V5</accession>
    <name>A0A2K2D5V5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RWP-RK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC104584323</name>
        <name evidence="8" type="ORF">BRADI_3g59420v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756080.1">
        <property type="nucleotide sequence ID" value="XM_014900594.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D5V5"/>
    <dbReference type="STRING" id="15368.A0A2K2D5V5"/>
    <dbReference type="EnsemblPlants" id="PNT69647">
        <property type="protein sequence ID" value="PNT69647"/>
        <property type="gene ID" value="BRADI_3g59420v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69648">
        <property type="protein sequence ID" value="PNT69648"/>
        <property type="gene ID" value="BRADI_3g59420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584323"/>
    <dbReference type="Gramene" id="PNT69647">
        <property type="protein sequence ID" value="PNT69647"/>
        <property type="gene ID" value="BRADI_3g59420v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69648">
        <property type="protein sequence ID" value="PNT69648"/>
        <property type="gene ID" value="BRADI_3g59420v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584323"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044607">
        <property type="entry name" value="RKD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003035">
        <property type="entry name" value="RWP-RK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373">
        <property type="entry name" value="PROTEIN RKD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373:SF29">
        <property type="entry name" value="RWP-RK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02042">
        <property type="entry name" value="RWP-RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51519">
        <property type="entry name" value="RWP_RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RWP-RK" evidence="7">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT69648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="151" mass="17980" checksum="1CD2292A5E7E11AC" modified="2018-03-28" version="1">MEKATKNEEHLSDGSQREKPSLRLEQVSQYFCMPLKQAAEELKVGQTTLKRSCRELRILRWPHRKVKSLQTLIENVQELEKDDVLGRSEKIRAVVQILQQRQKLMEERPAGVVELDKETRIFMQACYKEMYNRRRKGVKFAWMRSDRAGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D958</accession>
    <name>A0A2K2D958_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g18242v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D958"/>
    <dbReference type="EnsemblPlants" id="PNT70797">
        <property type="protein sequence ID" value="PNT70797"/>
        <property type="gene ID" value="BRADI_2g18242v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70797">
        <property type="protein sequence ID" value="PNT70797"/>
        <property type="gene ID" value="BRADI_2g18242v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D958"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT70797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="80" mass="8463" checksum="B2651742379E4159" modified="2018-03-28" version="1">MSAGVSRSLCASRLASSDVGDRSLAGDSLTPESGSTQRTATSGLPEESRERPATVLLCCFVSFCDDGLMAFLAVVDLFRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DDY2</accession>
    <name>A0A2K2DDY2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100836138</name>
        <name evidence="14" type="ORF">BRADI_2g45006v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232078.2">
        <property type="nucleotide sequence ID" value="XM_010233776.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DDY2"/>
    <dbReference type="FunCoup" id="A0A2K2DDY2">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DDY2"/>
    <dbReference type="EnsemblPlants" id="PNT72483">
        <property type="protein sequence ID" value="PNT72483"/>
        <property type="gene ID" value="BRADI_2g45006v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836138"/>
    <dbReference type="Gramene" id="PNT72483">
        <property type="protein sequence ID" value="PNT72483"/>
        <property type="gene ID" value="BRADI_2g45006v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836138"/>
    <dbReference type="OrthoDB" id="2396at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DDY2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07859">
        <property type="entry name" value="STKc_TDY_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000017">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000578">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="21"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="PNT72483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="494" mass="56572" checksum="7A9D0956D8A61ACC" modified="2018-03-28" version="1">MARKGSDEAEFFTEYGEANRYEVGEVVGKGSYGVVAAAVDTHTGERVAIKKINDVFEHVSDATRILREIKLLRLLRHPDIVEIKHIILPPSRREFRDIYIIFELMESDLHQVIKANDDLTPEHHQFFFYQLLRGMKYIHAANVFHRDLKPRNILANADCKLKICDFGLARVSFNDTPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIGCIFAEMLSGRPLFPGKNVVHQLDLMTDLLGTPSAESLSRIRNEKARRYLGNMRKKHPVPFSQKFPGVDPMALDLLERLLAFDPKDRPTAAEALADPYFTGLANSDREPTTQPISKLEFEFERRKLARDDVRELIYREILEYHPQMLHEYHHGGDQANFVYPSGVDRFKRQFVHLEEGVTKGEKTSPQLRQHASLPRERIIGIGDELGRPNADYCIKLHVGEEPGHTSVTDGLSKPLLNARNFLKSESISASQCVVIKEKREKDEESMSEYMHEASDATPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DFY8</accession>
    <name>A0A2K2DFY8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g54795v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DFY8"/>
    <dbReference type="EnsemblPlants" id="PNT73179">
        <property type="protein sequence ID" value="PNT73179"/>
        <property type="gene ID" value="BRADI_2g54795v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73179">
        <property type="protein sequence ID" value="PNT73179"/>
        <property type="gene ID" value="BRADI_2g54795v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DFY8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT73179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="86" mass="10065" checksum="3D92C2F5448DB138" modified="2018-03-28" version="1">MGTIHLFIHAHVIFRIFLNFYTSIQFFFKSSVLLRHFSGRSFLNRHIFVGMFAVTMYRVVAHSEAERQVEPRVEIGINQKYSTPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGL3</accession>
    <name>A0A2K2DGL3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_2g58231v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGL3"/>
    <dbReference type="STRING" id="15368.A0A2K2DGL3"/>
    <dbReference type="EnsemblPlants" id="PNT73422">
        <property type="protein sequence ID" value="PNT73422"/>
        <property type="gene ID" value="BRADI_2g58231v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73423">
        <property type="protein sequence ID" value="PNT73423"/>
        <property type="gene ID" value="BRADI_2g58231v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73422">
        <property type="protein sequence ID" value="PNT73422"/>
        <property type="gene ID" value="BRADI_2g58231v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73423">
        <property type="protein sequence ID" value="PNT73423"/>
        <property type="gene ID" value="BRADI_2g58231v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DGL3"/>
    <dbReference type="OrthoDB" id="442066at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DGL3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053211">
        <property type="entry name" value="DNA_repair-toleration"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48060">
        <property type="entry name" value="DNA DAMAGE-REPAIR/TOLERATION PROTEIN DRT100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48060:SF24">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014294233" evidence="8">
        <location>
            <begin position="28"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="9">
        <location>
            <begin position="34"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="77"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT73423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="537" mass="59455" checksum="9DC7C0FA71A6F070" modified="2018-03-28" version="1" fragment="single">MKDLHPVSLLMLSCTCLLLCSVSPAMARAGIRPEAEALVNWKASFASTDGSLGSWSLANSTGLCNWEYINCNSARHITELDLSAASLNGTLHQLDFSAFPHLKRLTLFEEGLYGTIPAGIGNLTSLVVLQITYSEYLSGSIPRSIGQLKHLVELRLKHLGLDSTLPEEIGNLTSLEELFLDSLTLTGSIPPTIERAKQQVRYLDLENNNLTGSIPLEIGNMTELRGMYFDVTIWKGSYRQNLLFGTLLTDQREVFFCNYTRLYFLDLSNNAFHGGLSKCFWDMPHLSFVDLTSNSFSGTVPFSRMCSLSYLHLANNHFKGTFPLVLKECKDLITLDLGGNSFSGAIPSWVSKSLPELKFLRLSSNMFDGAIPHEIVQFRFLQLLDLSKNKLAGPLPNDFANFTAMTREQKTTDYVYASYYMHPQQIQIVWKNVDHGIDLSGNSLSQEIPNGLTTLLGLMYLNLSGNHLSGCIPKDIGNLVLLEALDLSENQLSGEIPLSFADLKGMSALNLSSNGLSGRIPTGIVLFVTLQRFNIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DI96</accession>
    <name>A0A2K2DI96_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g06376v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DI96"/>
    <dbReference type="EnsemblPlants" id="PNT74002">
        <property type="protein sequence ID" value="PNT74002"/>
        <property type="gene ID" value="BRADI_1g06376v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74002">
        <property type="protein sequence ID" value="PNT74002"/>
        <property type="gene ID" value="BRADI_1g06376v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DI96"/>
    <dbReference type="OrthoDB" id="721765at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF48">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT74002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="366" mass="39965" checksum="26CAB9D01275E5AE" modified="2018-03-28" version="1">MALKRYAVPRCTLAGDATAKKPKEKERPPEISNADWAADVTRRNIESASRRERERRAKERNADLTRQMEAQRVAASAHMAAQRGGAAASAQMAGIPVPRPPSGQHWSSCSQVSSSSSSPSPAGISLVSPHMPHYHQGNATPSLSLFSPDYPDTDPLGGFNPNTFATDPLGGFNPNFASPPLWRGPLSYGGSSPSASFQQFPAGCSQPVPNLFGGMSQGDSIMADMINHSSQHAHYTYTQEEEPYAAEDIEEREEWADGTEEPKKGGGGGRGPKWTAKEDECLAEAWKVVSLDPFTGANQSGDTYWRRVKTAYDECRVIIREFASVTHDRNESGLSHRRANDPTSVQQVAWHSRRGAPATRKRHQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DJM1</accession>
    <name>A0A2K2DJM1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g15513v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DJM1"/>
    <dbReference type="EnsemblPlants" id="PNT74483">
        <property type="protein sequence ID" value="PNT74483"/>
        <property type="gene ID" value="BRADI_1g15513v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74483">
        <property type="protein sequence ID" value="PNT74483"/>
        <property type="gene ID" value="BRADI_1g15513v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DJM1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036319239" evidence="1">
        <location>
            <begin position="20"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT74483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="64" mass="7342" checksum="FC92CC191CA0A326" modified="2018-03-28" version="1">MRLSADFLLVYWGRLLVLEQCLLMAHRSCLQETLLFPSCSFPSKPTMEGYSLCSFSFMTQQSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DJT0</accession>
    <name>A0A2K2DJT0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100843535</name>
        <name evidence="13" type="ORF">BRADI_1g16810v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DJT0"/>
    <dbReference type="FunCoup" id="A0A2K2DJT0">
        <property type="interactions" value="336"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DJT0"/>
    <dbReference type="EnsemblPlants" id="PNT74534">
        <property type="protein sequence ID" value="PNT74534"/>
        <property type="gene ID" value="BRADI_1g16810v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74534">
        <property type="protein sequence ID" value="PNT74534"/>
        <property type="gene ID" value="BRADI_1g16810v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DJT0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000013">
        <property type="entry name" value="probable protein phosphatase 2C 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="12">
        <location>
            <begin position="29"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="PNT74534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="440" mass="47115" checksum="D998119BF8EB954D" modified="2018-03-28" version="1">MAGAEREKARLPPALPLATLIGRELRAGGSERPALRYGHAGFAKRGEDYFLVKPDCLRVPGDPSTAFSVFAVFDGHNGVSAAVFSKEHLLEHVMSALPPDIGGREEWLQALPRALVAGFVKADIEFQRKGEVSGTTATLVVVDGFTVTVASVGDSRCILDTQGGELQLLTVDHRLEENVEERERVTASGGEVGRLNLFGGQEVGPLRCWPGGLCLSRSIGDMDVGEYIVPVPHVKQVKLSNVGGRLIIASDGIWDTLSNEAAAKSCRGLPAELAAKLVVKQALKKCGLKDDTTCVVVDIIPSDRLVSPQLSPKRNQNKLKSLLFGRRSHSSVGKFGGKSASFGSVEELFEEGSAMLEERLGRNLSLKAASPPSRCAICQADQEPFEGLMAENGGSHCSSPYTPWGGPYLCLECRKKKDAMEGKRSSCSTACSDHQKGCKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQC0</accession>
    <name>A0A2K2DQC0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_1g48605v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQC0"/>
    <dbReference type="STRING" id="15368.A0A2K2DQC0"/>
    <dbReference type="EnsemblPlants" id="PNT76475">
        <property type="protein sequence ID" value="PNT76475"/>
        <property type="gene ID" value="BRADI_1g48605v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76475">
        <property type="protein sequence ID" value="PNT76475"/>
        <property type="gene ID" value="BRADI_1g48605v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DQC0"/>
    <dbReference type="OrthoDB" id="637156at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF21">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT76475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="87" mass="9900" checksum="D6EE272E26196554" modified="2018-03-28" version="1">MGKRGSQKPAPKKKPKKLETTFTCPFCQPSDGVDIDLKLRIAVATCWACEETYATKAHALTEAPRRLYSEWIDECEKANQEDHMEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DTN2</accession>
    <name>A0A2K2DTN2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g66258v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DTN2"/>
    <dbReference type="STRING" id="15368.A0A2K2DTN2"/>
    <dbReference type="EnsemblPlants" id="PNT77637">
        <property type="protein sequence ID" value="PNT77637"/>
        <property type="gene ID" value="BRADI_1g66258v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77637">
        <property type="protein sequence ID" value="PNT77637"/>
        <property type="gene ID" value="BRADI_1g66258v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DTN2"/>
    <dbReference type="OrthoDB" id="40823at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT77637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="78" mass="9258" checksum="2B812C7135DBC472" modified="2018-03-28" version="1">MIWVHFWRMVFILSYTLLLTARIAAGWFYTSINYCSLCCLKCFIVFLVVVWVIQEFTTFHVLKYVILFTGVMNSLFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DUF3</accession>
    <name>A0A2K2DUF3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g70260v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DUF3"/>
    <dbReference type="FunCoup" id="A0A2K2DUF3">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77905">
        <property type="protein sequence ID" value="PNT77905"/>
        <property type="gene ID" value="BRADI_1g70260v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77905">
        <property type="protein sequence ID" value="PNT77905"/>
        <property type="gene ID" value="BRADI_1g70260v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DUF3"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000154">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF53">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT77905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="471" mass="49842" checksum="133E4923035526F1" modified="2018-03-28" version="1">MYFIPFPTPGHALPMSDLARLFASRGADATLVLTHGNAARLGAPVARAAAAGLRIRIHGLTLPADAAGLKGGHESADDLPTREAAGPFAVAVDLLAPLFADLLRSQPADAVVFDGVLPWAATAAPELGIPRYAFTGTGCFALSVQRSLLLHSPQEAVGSSEHEPFLVPGLPDEVRLTRSRLAEATLPGAVSREFLNRMFDGERATAGWVVNSFSGLEERYVAHYERETRKPVFAVGPVCLINSDGDDALDRGGRRGEAAARVLRWLDGKPARSVVYACFGSLTRFPREQVAELGAGLANSGASFVWVVGEHTDMAALVPEDEERGLVVRGWAPQVAILRHAAVRCFVTHCGWGAVTEAAAAGVPVVAWPVFAEQFYNEALVVGVVGTGVGAGAERGYVWGAEETGGVVVGRETLAEKVRAAMADEGLRRRAGEMGERARRAVEDGGSSYQAVGALLEDVRRRRRSRGGRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DUS4</accession>
    <name>A0A2K2DUS4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">ABC transporter B family member 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BRADI_1g72517v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with 1-naphthylphthalamic acid (NPA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A2K2DUS4">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DUS4"/>
    <dbReference type="EnsemblPlants" id="PNT78029">
        <property type="protein sequence ID" value="PNT78029"/>
        <property type="gene ID" value="BRADI_1g72517v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78029">
        <property type="protein sequence ID" value="PNT78029"/>
        <property type="gene ID" value="BRADI_1g72517v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827033"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DUS4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000066">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000205">
        <property type="entry name" value="ABC transporter B family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF11">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="748"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="790"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="870"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="896"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="973"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="110"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="431"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="750"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="1072"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="669"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="PNT78029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1318" mass="142120" checksum="22156537813E7AC4" modified="2018-03-28" version="1">MVRYGPTSTAGARTPVSREVSFSRDNHNKLYVTSAQPARQDLPSFGYDVSVTSYNSRSQYYGDEDDDGGGVGQYDFDDEDDGEVELRAGKAVSVTGLFKYSTPLDIVLLVLGCVGAMINGGSLPWYSYLFGNFVNKIVTSDKTQMMKDVRQISVYMVILAVIVVIGAYLEIMCWRIVAERSALRVRREYLKAVLRQEIGFFDTEVSTGEVMQSISSDVAQIQEVMGDKMAGFVHHVFTFIFGYVVGFKTSWRITLAVLAVTPLMMACGLAYKAIYGGLTAKEEASYQRAGNVAQQAISSIRTVLSFVMEDRLADKYADWLQRSSPIGVKMGFAKGAGMGMIYLVTYSQWALALWYGAKLVAQGEIKGGDAIACFFGVMVGGRGLALSLSYSAQFAQGTAAAGRVFEIIDRAPEIDPYGTAGRALSSVRGRIEFKDVEFAYPSRPDSLILYNLNLTVPAAKMLALVGVSGGGKSTVFALIERFYDPTRGTITLDGQELGSLNLKWLRSQIGLVGQEPILFATSIIENVMMGKENATRQEAVAACTKANAHTFVLGLPDGYDTQVGDRGTQMSGGQKQRIALARAIIREPRILLLDEPTSALDAESEAVVQQSIDRLSVGRTVLVIAHRLATVRNADTIAVLDRGAVVESGRHADLMTRNGPYAGLVKLASNSGRTESDKPDAATPGRGTYNNNSFTDDSGYDVSVSKSKYAGIRTIHEEEAETKDNDKAKDTRFRISEIWELQRREGPLLILGFLMGINAGAVFSVFPLLLGQAVQVYFDPDTEKMRRQVGYLALAVVGLGVACILTMTGQQGFCGWAGARLTMRVRDRLFRAIMRQEPAWFDEDDNAMGVLVTRLARDAVAFRSMFGDRYAVLLMAVGSAGVGLGICFGLDVRLTLVAMACTPLTLGASYLNLLINLGARSDDGAYARASSIAAGAVSNVRTVAALCAQGGIVGTFNRALDGPSAKAQRRSQYMGLILGISQGAMYGAYTVTLWAGAYFIKKGQSSFGDVSKIFLILVLSSFSVGQLAGLAPDTSGAPTAIAGILAILKRRPAISEEGSKRRAIKEGKPMDVELRKVVFAYPSRPEITVLNDFSLRVKSGSTVALVGASGSGKSTVVWLVQRFYDPLGGTVMVGGLDVRDLDLKWLRGECALVGQEPALFSGSIRENIGFGNPKASWAEIEDAAKEANIHKFIAGLPQGYDTQVGESGVQLSGGQKQRIAIARAILKGSRILLLDEASSALDLESEKHVQEALRRVSRRATTITVAHRLSTVREADRIAVVSAGRTVEFGSHDGLLASHRDGLYAAMVKAEIEAQAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0A341N6Y3</accession>
    <name>A0A341N6Y3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Single-stranded DNA binding protein Ssb-like OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EnsemblPlants" id="PNT72873">
        <property type="protein sequence ID" value="PNT72873"/>
        <property type="gene ID" value="BRADI_2g50139v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72873">
        <property type="protein sequence ID" value="PNT72873"/>
        <property type="gene ID" value="BRADI_2g50139v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="591239at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472:SF13">
        <property type="entry name" value="OB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472">
        <property type="entry name" value="OS05G0244600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="1">
        <location>
            <begin position="18"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="112" mass="11970" checksum="F5DF488A5848B936" modified="2025-10-08" version="1">MATATAKRKPVFVKVNQLRPATAGHTLVVKVLSSEAVPQKPGAGANPGPTGRPTRIAECLVGDETGSILCSARDEQGFAVDKWGLVEVTEPANFNVKEDNNFSLIEYELVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNL0</accession>
    <name>I1GNL0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g09487v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNL0"/>
    <dbReference type="FunCoup" id="I1GNL0">
        <property type="interactions" value="205"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13341">
        <property type="protein sequence ID" value="KQK13341"/>
        <property type="gene ID" value="BRADI_1g09487v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13341">
        <property type="protein sequence ID" value="KQK13341"/>
        <property type="gene ID" value="BRADI_1g09487v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S6W9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158942_0_0_1"/>
    <dbReference type="InParanoid" id="I1GNL0"/>
    <dbReference type="OMA" id="RCKGKSS"/>
    <dbReference type="OrthoDB" id="599354at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.10">
        <property type="entry name" value="Trypsin Inhibitor V, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000864">
        <property type="entry name" value="Prot_inh_pot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036354">
        <property type="entry name" value="Prot_inh_pot1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091:SF7">
        <property type="entry name" value="INHIBITOR I FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091">
        <property type="entry name" value="PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00280">
        <property type="entry name" value="potato_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00292">
        <property type="entry name" value="POTATOINHBTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54654">
        <property type="entry name" value="CI-2 family of serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00285">
        <property type="entry name" value="POTATO_INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK13341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="67" mass="7234" checksum="9009D344D7ACC372" modified="2012-06-13" version="1">MGDAKSSWPEVLGAPAEAAKQTILRDRPDVQVFIVPPGNSVTGDFNNKRVRVFVDSSNKVVKVPKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPB3</accession>
    <name>I1GPB3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ataxin-2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100829450</name>
        <name evidence="2" type="ORF">BRADI_1g11710v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560939.1">
        <property type="nucleotide sequence ID" value="XM_003560891.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPB3"/>
    <dbReference type="STRING" id="15368.I1GPB3"/>
    <dbReference type="EnsemblPlants" id="KQK13666">
        <property type="protein sequence ID" value="KQK13666"/>
        <property type="gene ID" value="BRADI_1g11710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829450"/>
    <dbReference type="Gramene" id="KQK13666">
        <property type="protein sequence ID" value="KQK13666"/>
        <property type="gene ID" value="BRADI_1g11710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829450"/>
    <dbReference type="eggNOG" id="ENOG502S1J2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108773_3_0_1"/>
    <dbReference type="OMA" id="CFQDPQF"/>
    <dbReference type="OrthoDB" id="1918588at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GPB3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040414">
        <property type="entry name" value="CID1/CID2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33790">
        <property type="entry name" value="OS05G0344200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33790:SF1">
        <property type="entry name" value="PROTEIN EARLY RESPONSIVE TO DEHYDRATION 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK13666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15517" checksum="DA84B3D92154A119" modified="2012-06-13" version="1">MEVARGSNGGKLNPWAEPFVPGSWCRPMAVEAEAEAEVDDFSPEWWRLVASSSSFRDRWLRDYGDLGLLDDADDAHEGDGRKEEVAAGKEGGGGEVAPWGIEKWWRAHVTQPEVPKYAEKAPKKVPGGARFSPRTIQQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRA0</accession>
    <name>I1GRA0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100838739</name>
        <name evidence="6" type="ORF">BRADI_1g18185v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant rapid alkalinization factor (RALF) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559814.1">
        <property type="nucleotide sequence ID" value="XM_003559766.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRA0"/>
    <dbReference type="EnsemblPlants" id="KQK14706">
        <property type="protein sequence ID" value="KQK14706"/>
        <property type="gene ID" value="BRADI_1g18185v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838739"/>
    <dbReference type="Gramene" id="KQK14706">
        <property type="protein sequence ID" value="KQK14706"/>
        <property type="gene ID" value="BRADI_1g18185v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838739"/>
    <dbReference type="eggNOG" id="ENOG502R3QA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127895_1_1_1"/>
    <dbReference type="OMA" id="YAALMRN"/>
    <dbReference type="OrthoDB" id="1613518at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008801">
        <property type="entry name" value="RALF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33136:SF81">
        <property type="entry name" value="RAPID ALKALINIZATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33136">
        <property type="entry name" value="RAPID ALKALINIZATION FACTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05498">
        <property type="entry name" value="RALF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0372">Hormone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094031" evidence="5">
        <location>
            <begin position="27"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK14706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="102" mass="10714" checksum="8E5FFB48B9345008" modified="2012-06-13" version="1">MAPRALPLPLLLVLVLAVGLFAAAHASAAGGDFGERRRWLQEDNSSSTAGAASLLMGYISYSALFADSVPCSRQGASYYNCQPGAEANPYQRGCSAITQCRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSF1</accession>
    <name>I1GSF1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Exportin-T</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">Exportin(tRNA)</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 13">tRNA exportin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100830083</name>
        <name evidence="16" type="ORF">BRADI_1g21620v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <text evidence="13">Shuttles between the nucleus and the cytoplasm.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235692.1">
        <property type="nucleotide sequence ID" value="XM_010237390.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSF1"/>
    <dbReference type="STRING" id="15368.I1GSF1"/>
    <dbReference type="EnsemblPlants" id="KQK15279">
        <property type="protein sequence ID" value="KQK15279"/>
        <property type="gene ID" value="BRADI_1g21620v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK15280">
        <property type="protein sequence ID" value="KQK15280"/>
        <property type="gene ID" value="BRADI_1g21620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830083"/>
    <dbReference type="Gramene" id="KQK15279">
        <property type="protein sequence ID" value="KQK15279"/>
        <property type="gene ID" value="BRADI_1g21620v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15280">
        <property type="protein sequence ID" value="KQK15280"/>
        <property type="gene ID" value="BRADI_1g21620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830083"/>
    <dbReference type="eggNOG" id="KOG2021">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004414_1_1_1"/>
    <dbReference type="OMA" id="HEMFLFG"/>
    <dbReference type="OrthoDB" id="26399at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GSF1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000295">
        <property type="entry name" value="Exportin-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045546">
        <property type="entry name" value="Exportin-T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040017">
        <property type="entry name" value="XPOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952:SF11">
        <property type="entry name" value="EXPORTIN-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952">
        <property type="entry name" value="EXPORTIN-T/LOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19282">
        <property type="entry name" value="Exportin-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="6 13" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="14">
        <location>
            <begin position="102"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-T C-terminal" evidence="15">
        <location>
            <begin position="358"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF19282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK15280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="980" mass="108167" checksum="1D518A7278C0FF3A" modified="2012-06-13" version="1">MDDLEKAILLASDSPAAASASPAVREEALAFCARARDESPPSSLLRLCLSGLASSPHAHVHFWCLQSLHDALLRRRLALPDDLALLRSSLLSLASASNAASPPFLRNKLAQLIALLVRLDYPHVYPSYFLDLLPPSPPQSGPADMFARVLISLDDDLLSQDYPRNADEAADAGRVKDAMRAQCVPQIALHWHDAAVSLRAADPATAAVALDAARRCISWIDVALVANDVFVPLLFDIALSPGSAAPLAAAAVGCLSAVAAKRMDARAKVALLRSLLSAQQGLGSPDSGLKMATLVTTYAAEALECYRKLGPSDTDGTVALEMLEEVLPAVFSAAESSEEEEVDSGSVLEFLAGYVSTMKAPSDKQLGHLGRILEVVRQQMSYDPVYREHLDVPDKIGKEEEDLMAEQRKDLVALFRNICRVAPSATQQFIRGLMVTALSSAEATVEDVEVTLALFYRLGETVGEEEIRTGSGLLGELVPMLLSARFSCHTHRIVALVYLETVTRYIKFMQENVQYVPHLLAAYLDERGIHHQNSHVSRRAGYLFMKAVKLLKARLVPYLDTILQSLEDVLGQFTAMDWGNKDAKFSSSEDGSQVFEAVGLLIGIEEVSPEKQVQCLTALLNPLCHQIESLVMGAKAQGLEESSPRAMSLQQIVVALNMLTKGFNERLVMVSRPTIGVMFKKTLDVVLQVLISFPNMRSLRSKVISFLHRMIEILGISVLPCIPVALRQLLVNNEAKDMVDFLVLINQIISKFNSSASGILEDVFPTIASRLSVILSQDAFSTGPASNTEEMRELQELQRTLYTFLHAMVTHDLSTILLAPTCRQYLETIMQLLLFTSCSHKDILLRKACVQIFVQLIKDWCTTSKVDDKLPGFRVFMIGKFATGCCLYSVLDKSFDLRDANSLVLFGEIVMAQKIMYERFREDFVVNFVVKDLPEAHCPPDLAEQYYQKLQGNDIKAFRSFYQSLIEKIRQQQNGSLVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTM5</accession>
    <name>I1GTM5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100826112</name>
        <name evidence="8" type="ORF">BRADI_1g25230v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562948.1">
        <property type="nucleotide sequence ID" value="XM_003562900.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GTM5"/>
    <dbReference type="STRING" id="15368.I1GTM5"/>
    <dbReference type="EnsemblPlants" id="KQK15841">
        <property type="protein sequence ID" value="KQK15841"/>
        <property type="gene ID" value="BRADI_1g25230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826112"/>
    <dbReference type="Gramene" id="KQK15841">
        <property type="protein sequence ID" value="KQK15841"/>
        <property type="gene ID" value="BRADI_1g25230v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826112"/>
    <dbReference type="eggNOG" id="ENOG502SPJG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108216_0_0_1"/>
    <dbReference type="OMA" id="ATENMPH"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GTM5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051358">
        <property type="entry name" value="TF_AMS/ICE1/BHLH6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945">
        <property type="entry name" value="TRANSCRIPTION FACTOR SCREAM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945:SF68">
        <property type="entry name" value="TRANSCRIPTION FACTOR UDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="56"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK15841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="229" mass="25197" checksum="0C7B14CEBF829A98" modified="2012-06-13" version="1">MPRRPRAPRGGGSGGGEEVKMEDFVESMLNFGGGGGGGGEDESEEGEQLPAGEETKYKSKNLDAERRRRGRLNNNILALRAVVPKITKMSKESTLSDAIDHIKKLQNEVLELQSQLADSPGEAWEKQGSASCSESFVPTDNIHYQGQVELIPLGSFKYNLKIFWTKKAGLFTKVLEALCSYNVQVLSLNTITFYGYAESFFSIEVKGEPDVVMVELRNLLSSIVEVPSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWL4</accession>
    <name>I1GWL4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100838878</name>
        <name evidence="4" type="ORF">BRADI_1g33950v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563490.1">
        <property type="nucleotide sequence ID" value="XM_003563442.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWL4"/>
    <dbReference type="STRING" id="15368.I1GWL4"/>
    <dbReference type="EnsemblPlants" id="KQK17358">
        <property type="protein sequence ID" value="KQK17358"/>
        <property type="gene ID" value="BRADI_1g33950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838878"/>
    <dbReference type="Gramene" id="KQK17358">
        <property type="protein sequence ID" value="KQK17358"/>
        <property type="gene ID" value="BRADI_1g33950v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838878"/>
    <dbReference type="eggNOG" id="KOG4210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066544_0_1_1"/>
    <dbReference type="OMA" id="KHVNSAG"/>
    <dbReference type="OrthoDB" id="696276at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051992">
        <property type="entry name" value="OxStress_Response_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33172:SF90">
        <property type="entry name" value="OS06G0698748 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33172">
        <property type="entry name" value="OS08G0516900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="72"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="173"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK17358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="213" mass="21457" checksum="5408086BBB1D7639" modified="2012-06-13" version="1">MSVVAAAAVGPPAPVVAPAPAVAPFGLYGGGGGGGARKRKDVVRPEETAEDGGGGVHGLFVLETVVEEEAEAEGERSSIGAVSDDEAVEDGDEAESSPGQRKKKAGLACLDALDDALPIKRGLSSFFSGKSRSFANLQDVVAVAGETGGANVLAKPENPFNKRRRVLRCSSMRRVSSTSLTALPPFLPPGPRPSSFNIGNDDSATNGGGGGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXA2</accession>
    <name>I1GXA2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100827354</name>
        <name evidence="11" type="ORF">BRADI_1g35920v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563620.1">
        <property type="nucleotide sequence ID" value="XM_003563572.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXA2"/>
    <dbReference type="FunCoup" id="I1GXA2">
        <property type="interactions" value="1251"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GXA2"/>
    <dbReference type="EnsemblPlants" id="KQK17660">
        <property type="protein sequence ID" value="KQK17660"/>
        <property type="gene ID" value="BRADI_1g35920v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827354"/>
    <dbReference type="Gramene" id="KQK17660">
        <property type="protein sequence ID" value="KQK17660"/>
        <property type="gene ID" value="BRADI_1g35920v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827354"/>
    <dbReference type="eggNOG" id="KOG1677">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045191_0_0_1"/>
    <dbReference type="OMA" id="LDNSMPH"/>
    <dbReference type="OrthoDB" id="361242at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GXA2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22464">
        <property type="entry name" value="KH-I_AtC3H36_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000069">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF154">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK17660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="298" mass="31104" checksum="9BAC91F07F0B4812" modified="2012-06-13" version="1">MDAGRKRAVPEGTNGGGAAKRPRESESFQMGVGSKSKPCTKFFSTAGCPFGSGCHFLHNFPGGHQAVSKMTNLGGPAVATPPGRMPMGPGVPDGPPTPGVKTRMCNKFNTAEGCKWGNKCHFAHGERELGKPMLLNNSMVPPMGPRPNGHFVPPPMPAPDMVPPSTFGASATAKISVDASLAGAIIGKGGVNTKHISRITGAKLAIRDNEADPNFKNIELEGTFDQIKYASSMVTDLIVRIGGNAPPAKNPTRGPHVGGGGNNFKTKLCDNFSKGSCTFGDRCHFAHGESELRKSVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1H1A1</accession>
    <name>I1H1A1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SWN7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">SWN7</name>
        <name evidence="8" type="ORF">BRADI_1g50057v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Valdivia E."/>
                <person name="Herrera M.T."/>
                <person name="Gianzo C."/>
                <person name="Revilla G."/>
                <person name="Zarra I."/>
                <person name="Sampedro J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="journal article" date="2013" name="J. Exp. Bot." volume="64" first="1333" last="1343">
            <title>Regulation of secondary wall synthesis and cell death by NAC transcription factors in the monocot Brachypodium distachyon.</title>
            <authorList>
                <person name="Valdivia E.R."/>
                <person name="Herrera M.T."/>
                <person name="Gianzo C."/>
                <person name="Fidalgo J."/>
                <person name="Revilla G."/>
                <person name="Zarra I."/>
                <person name="Sampedro J."/>
            </authorList>
            <dbReference type="PubMed" id="23386682"/>
            <dbReference type="DOI" id="10.1093/jxb/ers394"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="5">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
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        <property type="protein sequence ID" value="AFJ91967.1"/>
        <property type="molecule type" value="mRNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="PNT76596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NM_001279906.1"/>
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    <dbReference type="STRING" id="15368.I1H1A1"/>
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        <property type="gene ID" value="BRADI_1g50057v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT76596"/>
        <property type="gene ID" value="BRADI_1g50057v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830276"/>
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        <property type="protein sequence ID" value="KQK19730"/>
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        <property type="gene ID" value="BRADI_1g50057v3"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF221">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 43-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="11"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="201"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFJ91967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT76596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="415" mass="44463" checksum="CE94A2C4F13DF258" modified="2012-06-13" version="1">MSISVNGQSVVPPGFRFHPTEEELLTYYLAKKVASCRIDLDVIPDVDLNKLEPWDIQERCRIGTGPQSDWYLFSHKDKKYPTGTRTNRATAAGFWKATGRDKPIYSSSSGSGTGKIGMRKTLVFYKGRAPHGTKSDWIMHEYRLHDNAQNHHSSPGAASSSPIRGGDQSAAQQEEGWVICRVFKKKNLICCNNNNNQALASHPQISSNNNKQPSPSRSCSAVSDDHHAASRAAAAQMLHHYSAGGSGDVDTHTLDHILRYMGSNSGKQESKPAPLTSACSLPNMDQYSLVNGGGGGTLYDKFMKLPPLEHVVVSGSGGGLLQLPPPTPQGSQYGGADCWADTLAAYELNGGLSDNDVDGSTKTTNGGMSTPGCFFVAEQHHQHGHGGGGDGDLWSLARSSVSTLHADLATAMDNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1H1Z9</accession>
    <name>I1H1Z9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MADS-box transcription factor 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104581664</name>
        <name evidence="3" type="ORF">BRADI_1g52060v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2014" name="PLoS ONE" volume="9" first="e84781" last="e84781">
            <title>Genome-wide analysis of the MADS-box gene family in Brachypodium distachyon.</title>
            <authorList>
                <person name="Wei B."/>
                <person name="Zhang R.Z."/>
                <person name="Guo J.J."/>
                <person name="Liu D.M."/>
                <person name="Li A.L."/>
                <person name="Fan R.C."/>
                <person name="Mao L."/>
                <person name="Zhang X.Q."/>
            </authorList>
            <dbReference type="PubMed" id="24454749"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="KF469304">
        <property type="protein sequence ID" value="AIG21818.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001303842.1">
        <property type="nucleotide sequence ID" value="NM_001316913.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228134.1">
        <property type="nucleotide sequence ID" value="XM_010229832.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H1Z9"/>
    <dbReference type="STRING" id="15368.I1H1Z9"/>
    <dbReference type="EnsemblPlants" id="KQK20037">
        <property type="protein sequence ID" value="KQK20037"/>
        <property type="gene ID" value="BRADI_1g52060v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT76716">
        <property type="protein sequence ID" value="PNT76716"/>
        <property type="gene ID" value="BRADI_1g52060v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581664"/>
    <dbReference type="Gramene" id="KQK20037">
        <property type="protein sequence ID" value="KQK20037"/>
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    </dbReference>
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        <property type="protein sequence ID" value="PNT76716"/>
        <property type="gene ID" value="BRADI_1g52060v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34212:SF2">
        <property type="entry name" value="MADS-BOX TRANSCRIPTION FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34212">
        <property type="entry name" value="OS02G0104200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AIG21818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK20037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="226" mass="24663" checksum="FF3CB8222141CF51" modified="2012-06-13" version="1">MDRAGGNQAGQVLKKGKKKHAKDESDRQKQAEKKRRRLEKALANSAAIISELEKKRRQKQEEQQRLDDEGAAIAEAVALHVLIGEDCDEPCQLMLNNRKRCDGFLNLELTPGAQGAGDGGYLCGSRLARGSHAHVPQWRWTDCGPFSFSSWEEVGDFEALYRRGAFAQSDSDTCPDHVAAWAVSPLQGGVGSCEDAFPVQGAAASAINIMLSDTTTTSSLNIYREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1H3H7</accession>
    <name>I1H3H7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histone deacetylase</fullName>
            <ecNumber evidence="4">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100831369</name>
        <name evidence="18" type="ORF">BRADI_1g56740v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:58197"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H3H7"/>
    <dbReference type="STRING" id="15368.I1H3H7"/>
    <dbReference type="EnsemblPlants" id="KQK20778">
        <property type="protein sequence ID" value="KQK20778"/>
        <property type="gene ID" value="BRADI_1g56740v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK20781">
        <property type="protein sequence ID" value="KQK20781"/>
        <property type="gene ID" value="BRADI_1g56740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20778">
        <property type="protein sequence ID" value="KQK20778"/>
        <property type="gene ID" value="BRADI_1g56740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20781">
        <property type="protein sequence ID" value="KQK20781"/>
        <property type="gene ID" value="BRADI_1g56740v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1343">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="VCGEHRE"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H3H7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09992">
        <property type="entry name" value="HDAC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000014">
        <property type="entry name" value="Histone deacetylase 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF5">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="17">
        <location>
            <begin position="140"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK20778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="644" mass="69146" checksum="152AC3DBF9A5B871" modified="2012-06-13" version="1">MASDMRPPNSKKGGVPSHARVSDQACHDKFQSCDGDGKPSRAAVNGVSVKENHGTCSSNPDHAGAVLVDGAKGSIAQSGHVDSADYDCAGCLHGENGSPMACDLQQESDRDQAGHTLDDLFFFDDEEEDDIDWEPSARIVENRWFCTNCTMPNMDEVTHCQNCNDHKGSEVLAGYDVFQASFAQAALISADTALPPGSTAIGFDEQMLLHSEVEIKPNPHPERPNRLRAIAASLAAAGIFPSKCALVPPREITKKELLMVHTSDHVESVEQTKNMLYSYFTSDTYANKESARAAKLAAGLCADLASLIVSGRVNNGFALVRPPGHHAGVKQAMGFCLHNNAAVAALAAQKAGAKKVLIVDWDVHHGNGTQEIFEGNKSVLYISLHRHEDGNFYPGTGAAHEVGVLDGQGFSVNIPWSCGGVGDDDYIFAFQHVVLPIAAEFAPDITIVSAGFDAARGDPLGCCDVTPAGYSQMTSMLTTCSEGRLLIILEGGYNLRSISSSATEVVKVLLGDGPCYGKGAAAPSKEGMQTVLQVLEIQQQYWPVLVPILASLQAQQGLALSKYECVSRELRRGMCRKNFFVGWRREPFPSLTPRCPRTAGNRCQWLPTSTIRLRLISQGSSLECGTSGRRDSDMCRLCSSSVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4H9</accession>
    <name>I1H4H9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_1g59660v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Plant hormone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H4H9"/>
    <dbReference type="STRING" id="15368.I1H4H9"/>
    <dbReference type="EnsemblPlants" id="KQK21247">
        <property type="protein sequence ID" value="KQK21247"/>
        <property type="gene ID" value="BRADI_1g59660v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21247">
        <property type="protein sequence ID" value="KQK21247"/>
        <property type="gene ID" value="BRADI_1g59660v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_15_5_1"/>
    <dbReference type="InParanoid" id="I1H4H9"/>
    <dbReference type="OMA" id="CPTAFLV"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000022">
        <property type="entry name" value="Taxadiene 5-alpha hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF364">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK21247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="53831" checksum="0EB66FB6EE9966E7" modified="2012-06-13" version="1">MEMDASVLAPLVALFLASFFLVLRKHRSSPPDDRRHQQLPPGSFGLPVIGQTLSFLSALRGNTGEAWLRQWAAVYGPVSRFSFFGVRTALIVGPSANKFLFSSAGLTAKTTTAMNRMVGRRNIRELTGNNHRHVRAMFVQFLKLDVVRGYVGAIDDEVRHHLSANWHGHATIAVMPSMKTLTFDIMCTVIFGLDRGAHAAMRRDLSMEFQQLVRGVWSIPVNLPFTTFRKCLAASQRGRHTVAKIIDEKRRKLERGESSSSNDLMTHMLAEGMADDDIIDNIMFMILAAHDTTATLLTFLIRHLDSNKDAYAKVIAEQEEVARSKARGEALSWDDLGKMRYTWAAAMETLRMVPPIFGNFKRVVEDVEFNGHLIPKGWQVMTAMNLTQWDPAIFPDPGRFDPTRFESPLPPYSFVAFGGGGRVCPGNEFARVEALVAMHYIITGFRWKLADGCDGSFSRYPLPSPAQGLLIDIEPKDTTTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6E5</accession>
    <name>I1H6E5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100845835</name>
        <name evidence="2" type="ORF">BRADI_1g65060v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558119.1">
        <property type="nucleotide sequence ID" value="XM_003558071.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6E5"/>
    <dbReference type="STRING" id="15368.I1H6E5"/>
    <dbReference type="EnsemblPlants" id="KQK22081">
        <property type="protein sequence ID" value="KQK22081"/>
        <property type="gene ID" value="BRADI_1g65060v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK22082">
        <property type="protein sequence ID" value="KQK22082"/>
        <property type="gene ID" value="BRADI_1g65060v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845835"/>
    <dbReference type="Gramene" id="KQK22081">
        <property type="protein sequence ID" value="KQK22081"/>
        <property type="gene ID" value="BRADI_1g65060v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22082">
        <property type="protein sequence ID" value="KQK22082"/>
        <property type="gene ID" value="BRADI_1g65060v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845835"/>
    <dbReference type="eggNOG" id="ENOG502S1DT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111786_0_0_1"/>
    <dbReference type="OMA" id="ARIWMEK"/>
    <dbReference type="OrthoDB" id="2384430at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36792">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36792:SF5">
        <property type="entry name" value="SEL1 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="157" mass="16982" checksum="B8A755814C11DC8B" modified="2012-06-13" version="1">MGKRLPAAALAAAFRPYARSSPIASKAAKPPTAPLDTPRNAGAGAGASSGRAEVRDLAAACGMQEHERVPLGEVVLDCTKRWFQDSLKEARAGDAAMQVLVGQMYRSGYGVNKNEHKARIWMEKASRYRSTVWKVSNKRPGYNASDSDTDDIQETSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1H807</accession>
    <name>I1H807_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">plant cystathionine gamma-synthase</fullName>
            <ecNumber evidence="7">2.5.1.160</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100842309</name>
        <name evidence="11" type="ORF">BRADI_1g69730v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-homoserine + L-cysteine = L,L-cystathionine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:80891"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="EC" id="2.5.1.160"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-succinyl-L-homoserine + L-cysteine = L,L-cystathionine + succinate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20397"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57661"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.160" evidence="7"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561880.1">
        <property type="nucleotide sequence ID" value="XM_003561832.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H807"/>
    <dbReference type="STRING" id="15368.I1H807"/>
    <dbReference type="EnsemblPlants" id="KQK22864">
        <property type="protein sequence ID" value="KQK22864"/>
        <property type="gene ID" value="BRADI_1g69730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842309"/>
    <dbReference type="Gramene" id="KQK22864">
        <property type="protein sequence ID" value="KQK22864"/>
        <property type="gene ID" value="BRADI_1g69730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842309"/>
    <dbReference type="eggNOG" id="KOG0053">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_1_0_1"/>
    <dbReference type="OMA" id="EVTWTDP"/>
    <dbReference type="OrthoDB" id="588613at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003962">
        <property type="term" value="F:cystathionine gamma-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000046">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000033">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044639">
        <property type="entry name" value="CGS1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43379:SF3">
        <property type="entry name" value="CYS_MET METABOLISM PLP-DEPENDENT ENZYME FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43379">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK22864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="467" mass="51134" checksum="CBE642A7A7AA5FB0" modified="2012-06-13" version="1">MARSSSAVKSSLPKQRRSLRPRRSPKPNFQRHAAALNPDALVDATLPVFEPEANHPGCADQDMVVPTAARRRRSVSDETLAVHAGEKLGKDGMAETDSIATPVVSGTTHWFRSSEDLIAFKEGRRHSFEYGRYGNPTVKVLEEKISALERAEATLVTSSGMNAIVATLLALVPPGGHVVTTNDCYSEARAFIRDRLSKMGIRSTFVDLDDIETLKAVLDQGDVTLFYADSPTNPHLKCIDIKLVAELCHRKGALVCIDSTLSSPINQKPLTLGADIVVHSATKYIAGHHDVIAGCISGSDALLSRIRAWHHDLGGAISPDAAYMIIRGLKTMALRVETQNRTALRMARLLENHPKIERVYYPGLKSSPWHHVAKSQMTGFGGVISFEVASDLRGVMRFVDALEIPFIATSLGGCESLVQQPAVMSFWGQSDEEKSKNGITDNLVRFSFGIEKFEDLRDDILQALEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAQ0</accession>
    <name>I1HAQ0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="6">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100834548</name>
        <name evidence="9" type="ORF">BRADI_1g77990v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Extracellular space</location>
            <location evidence="6">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="6"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559007.1">
        <property type="nucleotide sequence ID" value="XM_003558959.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAQ0"/>
    <dbReference type="FunCoup" id="I1HAQ0">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HAQ0"/>
    <dbReference type="EnsemblPlants" id="KQK24074">
        <property type="protein sequence ID" value="KQK24074"/>
        <property type="gene ID" value="BRADI_1g77990v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834548"/>
    <dbReference type="Gramene" id="KQK24074">
        <property type="protein sequence ID" value="KQK24074"/>
        <property type="gene ID" value="BRADI_1g77990v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834548"/>
    <dbReference type="eggNOG" id="ENOG502QQC7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048041_1_1_1"/>
    <dbReference type="OMA" id="YRADYAY"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000025">
        <property type="entry name" value="Xyloglucan endotransglucosylase/hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094424" description="Xyloglucan endotransglucosylase/hydrolase" evidence="7">
        <location>
            <begin position="42"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="8">
        <location>
            <begin position="39"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK24074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="318" mass="35236" checksum="C8CF082EE25CB933" modified="2012-06-13" version="1">MMMMRVIRRCSDNEDGGAASSVVALVRAAALLVALMQPAAAQQVPSPGYYPSSTVRSMAFSQGYTNLWGPQHQSLSPDQSSLTLFMDRSSGSGFKSKKSYRSGYFGVSIKVQPGYTAGVNTAFYLSNSELYPGQHDEIDMELLGTIPGEPYTLQTNVYVHGTGDSNPIIGREMRFHLWFDPTADFHHYAILWDQNQVVFLVDDVPIRRYQRKPGAAALFPGREMWAYGSVWDASDWATDGGKYKADYKYAPFVARFSEFKLAGCEVGGPDGCRPVATGPGGGGLSGKQEETMRWAQQRSMVYYYCTDRSKNRANYPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HGT7</accession>
    <name>I1HGT7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">threonine synthase</fullName>
            <ecNumber evidence="7">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100839814</name>
        <name evidence="20" type="ORF">BRADI_2g17620v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-homoserine + H2O = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="7"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567955.1">
        <property type="nucleotide sequence ID" value="XM_003567907.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HGT7"/>
    <dbReference type="FunCoup" id="I1HGT7">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HGT7"/>
    <dbReference type="EnsemblPlants" id="KQK05047">
        <property type="protein sequence ID" value="KQK05047"/>
        <property type="gene ID" value="BRADI_2g17620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839814"/>
    <dbReference type="Gramene" id="KQK05047">
        <property type="protein sequence ID" value="KQK05047"/>
        <property type="gene ID" value="BRADI_2g17620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839814"/>
    <dbReference type="eggNOG" id="ENOG502QSQC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_5_0_1"/>
    <dbReference type="OMA" id="HLAGMND"/>
    <dbReference type="OrthoDB" id="7773036at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01563">
        <property type="entry name" value="Thr-synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000030">
        <property type="entry name" value="Threonine synthase 1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="15" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0934">Plastid</keyword>
    <keyword evidence="13 17" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="12" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="14" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="19">
        <location>
            <begin position="163"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="17">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KQK05047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="525" mass="57487" checksum="7EDEB61A8C21EF53" modified="2012-06-13" version="1">MAAAATHASSLSFLLSRPHSSSPHQLRLRRPADHRVRCATDASAAAAAPAKHRRAADENIREEAARHPAPNNDFSAWYEPFPPGAPGGDNPDERYSLDEIVYRSSSGGLLDVRHDMEALARFPGSYWRDLFDSRIGRTTWPYGSGVWSKKEFVLPEIDPEHIVSLFEGNSNLFWAERLGRDHLGGMKDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRRAPLSRPIAGVGCASTGDTSAALSAYCAAAGIPAIVFLPANRISLEQLIQPIANGATVLSLDTDFDGCMRLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWIIVPGGNLGNIYAFYKGFEMCRVLGLVDRLPRLVCAQAANANPLYRYYKSGWTEFQPQVAEPTFASAIQIGDPVSVDRAVVALKATNGIVAEATEEELMNAMSLADRTGMFACPHTGVALAALFKLREQRVIGPNDRTVVVSTAHGLKFSQSKIDYHDRKIEDMACKYANPPVSVKADFGAVMDVLKTRLKGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HHR6</accession>
    <name>I1HHR6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100834702</name>
        <name evidence="10" type="ORF">BRADI_2g20160v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566053.1">
        <property type="nucleotide sequence ID" value="XM_003566005.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HHR6"/>
    <dbReference type="STRING" id="15368.I1HHR6"/>
    <dbReference type="MEROPS" id="A01.A60"/>
    <dbReference type="EnsemblPlants" id="KQK05459">
        <property type="protein sequence ID" value="KQK05459"/>
        <property type="gene ID" value="BRADI_2g20160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834702"/>
    <dbReference type="Gramene" id="KQK05459">
        <property type="protein sequence ID" value="KQK05459"/>
        <property type="gene ID" value="BRADI_2g20160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834702"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005738_8_1_1"/>
    <dbReference type="OMA" id="PQGNHFY"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000098">
        <property type="entry name" value="Chloroplast nucleoid DNA-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000055">
        <property type="entry name" value="Probable aspartyl protease At4g16563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF47">
        <property type="entry name" value="CHLOROPLAST NUCLEOID DNA-BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="9">
        <location>
            <begin position="25"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="331"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK05459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="441" mass="46999" checksum="73DD006EACD8C935" modified="2012-06-13" version="1">MGLAYSTKFDFLDIIEPIATYTDGYLLSLNLGTPPQVFQVYLDTGSDLTWVPCGTNTSYQCLECGNEHSISKPTPAFSLSQSYSSTRDLCGSRFCVDVHSSDNSHDACAAAGCSIPVFMSGLCTRLCPPFAYTYGGRALVLGSLARDTIALHGSIYGISVPIEFPGFCFGCVGSSIREPIGIAGFGKGKLSLPSQLGFLDKGFSHCFLGFWFARNPNITSPMVIGDLALSVKDGFLFTPMLKSLTYPNFYYIGLEGVTIGDNAAIPAPPSLSGIDSEGNGGVIVDTGTTYTHLSDPFYASVLSSLSSTVPYNRSYELEIRTGFDLCLKVPCMHAPCNDDELPPITVHLGGDVTLALPKESCYYAVTAPRNSVVIKCLLFQRKDDDGVFSADNDDGEDASFSAGGPAAVLGSFQMQNVEVVYDLESGRVGFQPRDCALGVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1HIK8</accession>
    <name>I1HIK8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100829384</name>
        <name evidence="6" type="ORF">BRADI_2g22800v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566168.1">
        <property type="nucleotide sequence ID" value="XM_003566120.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314137.1">
        <property type="nucleotide sequence ID" value="XM_024458369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HIK8"/>
    <dbReference type="STRING" id="15368.I1HIK8"/>
    <dbReference type="EnsemblPlants" id="KQK05831">
        <property type="protein sequence ID" value="KQK05831"/>
        <property type="gene ID" value="BRADI_2g22800v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT71081">
        <property type="protein sequence ID" value="PNT71081"/>
        <property type="gene ID" value="BRADI_2g22800v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT71082">
        <property type="protein sequence ID" value="PNT71082"/>
        <property type="gene ID" value="BRADI_2g22800v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829384"/>
    <dbReference type="Gramene" id="KQK05831">
        <property type="protein sequence ID" value="KQK05831"/>
        <property type="gene ID" value="BRADI_2g22800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71081">
        <property type="protein sequence ID" value="PNT71081"/>
        <property type="gene ID" value="BRADI_2g22800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71082">
        <property type="protein sequence ID" value="PNT71082"/>
        <property type="gene ID" value="BRADI_2g22800v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829384"/>
    <dbReference type="eggNOG" id="ENOG502QUBD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073450_0_0_1"/>
    <dbReference type="OMA" id="GAANWQH"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045281">
        <property type="entry name" value="CONSTANS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319:SF115">
        <property type="entry name" value="OS05G0466100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51017">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCT" evidence="5">
        <location>
            <begin position="177"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK05831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="314" mass="35100" checksum="99E1C17005F1AC34" modified="2012-06-13" version="1">MFNPSSSSVPTYNDLSMHHAVSFSTASLPTAPTEIPRRSSGFFHDNGAVISPSNVAASAPPPYPSSLPSYYVHNTQSFPFHLQYPDALNRNATFSCQSPSACQVHPPPASYSPSSSSGGFLEFCPGTMRRVFSAGDLQGKNVSPPTPPPPQFSGDNCSQEVVEPFLEKVGRYSTEERKERIERYRTKRQQRNFQKKITYACRKTLADSRPRVQGRFARNVETDGEVVADFERDVGSDISYEYCSYNDLSSNSYDSQNQHRETGSSMTFNDNKWWWGMPVAANEHQQQQQPQQLGFNLDDEDQLWASLADMCSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1HIW6</accession>
    <name>I1HIW6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor MYBS1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Myb-related protein S1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100826230</name>
        <name evidence="13" type="ORF">BRADI_2g23710v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568340.1">
        <property type="nucleotide sequence ID" value="XM_003568292.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HIW6"/>
    <dbReference type="FunCoup" id="I1HIW6">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HIW6"/>
    <dbReference type="EnsemblPlants" id="KQK05980">
        <property type="protein sequence ID" value="KQK05980"/>
        <property type="gene ID" value="BRADI_2g23710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826230"/>
    <dbReference type="Gramene" id="KQK05980">
        <property type="protein sequence ID" value="KQK05980"/>
        <property type="gene ID" value="BRADI_2g23710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826230"/>
    <dbReference type="eggNOG" id="KOG0724">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060837_1_1_1"/>
    <dbReference type="OMA" id="MHCGQLQ"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009739">
        <property type="term" value="P:response to gibberellin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009744">
        <property type="term" value="P:response to sucrose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000009">
        <property type="entry name" value="transcription factor MYB1R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000154">
        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042:SF41">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="12">
        <location>
            <begin position="135"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="135"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="11">
        <location>
            <begin position="143"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="207"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK05980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="301" mass="32875" checksum="0CF0C52D2D6B7577" modified="2012-06-13" version="1">MMKESWMEVLPLPPAPYLAGGWFLQEQQRAWSPAENKLFEEALARVDGDAPGRWERVAALLPGKSVADVMAHYDDLENDVGFIEAGLVPFPQYNGGGGGSPASGFTLDWDGGGDDLAFKRSCYIVGGKRARGPDQERKKGVPWTEEEHKLFLMGLKKYGRGDWRNISRNFVTSRTPTQVASHAQKYFIRLNSGGKDKRRSSIHDITTVNIPDDDHGSNPSPSPPSSVLTPNSSSGAAPPAISEQFGVLVDSKPQISRGGHHHFIPHLYGNVKLEASNGYHGGRLDDSVLMQMQCGQLQPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HKZ1</accession>
    <name>I1HKZ1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100823880</name>
        <name evidence="12" type="ORF">BRADI_2g32900v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568756.1">
        <property type="nucleotide sequence ID" value="XM_003568708.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HKZ1"/>
    <dbReference type="EnsemblPlants" id="KQK07074">
        <property type="protein sequence ID" value="KQK07074"/>
        <property type="gene ID" value="BRADI_2g32900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823880"/>
    <dbReference type="Gramene" id="KQK07074">
        <property type="protein sequence ID" value="KQK07074"/>
        <property type="gene ID" value="BRADI_2g32900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823880"/>
    <dbReference type="eggNOG" id="ENOG502QT4C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025298_0_0_1"/>
    <dbReference type="OMA" id="PEISWMV"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HKZ1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009640">
        <property type="term" value="P:photomorphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19821">
        <property type="entry name" value="Bbox1_BBX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000589">
        <property type="entry name" value="B-box zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051979">
        <property type="entry name" value="B-box_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049808">
        <property type="entry name" value="CONSTANS-like_Bbox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31832">
        <property type="entry name" value="B-BOX ZINC FINGER PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31832:SF9">
        <property type="entry name" value="OS05G0204600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="61"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="117"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="174"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="239"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK07074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="333" mass="35192" checksum="D4150BD8F2A84CDC" modified="2012-06-13" version="1">MKVLCSACEAAEACVLCCADEAALCDRCDRDVHAANRLAGKHQRLPLLSPGSASADPAPPASPPKCDICQECHAYFFCLEDRALLCRGCDVAVHTANAFVSTHRRFLLTGVQVSLDEQEDDCLPDPPEPSPAPAPPPPAPAAKRDQASLYGEGDFSWAAATADANESLPDWSIVNEQFGSPAPRHAEAASASAAASRTPPKRAPAFSGQGGMMDWPLGEFFGGFSNFNAGFGFGFGESGTSKADSGKQGGSTGGSPYYVSSSDDRNADELFGQVPEMQRSAAELPSPPTASGLHWQRRPADYGAFVPDISSPDSSLRYCFPADQTAVKRRRKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HLT9</accession>
    <name>I1HLT9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100833483</name>
        <name evidence="6" type="ORF">BRADI_2g35840v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566555.1">
        <property type="nucleotide sequence ID" value="XM_003566507.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315275.1">
        <property type="nucleotide sequence ID" value="XM_024459507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HLT9"/>
    <dbReference type="EnsemblPlants" id="KQK07501">
        <property type="protein sequence ID" value="KQK07501"/>
        <property type="gene ID" value="BRADI_2g35840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833483"/>
    <dbReference type="Gramene" id="KQK07501">
        <property type="protein sequence ID" value="KQK07501"/>
        <property type="gene ID" value="BRADI_2g35840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833483"/>
    <dbReference type="eggNOG" id="KOG2458">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027109_2_0_1"/>
    <dbReference type="OMA" id="EDDCMFP"/>
    <dbReference type="OrthoDB" id="202415at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF35">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="5">
        <location>
            <begin position="195"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="473"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK07501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="493" mass="55048" checksum="FDDF34160314062F" modified="2012-06-13" version="1">MGGYIPESGTGTVVRAARAGAGRYPPLASLVVSTIAAFSAVIVIAVLHSAYDEAVSRTRTLLGHNLEPTPWHPFPHDKGRPPPRAALRCAPLLSCLPPLSHPHPSPPNASRSRRTKQCPAYFAAIHRDLAPWRRHGHGGITRELLDSARSRASMRVTITGNGRRLHVDLYYACVQSRALFTVWSLLQLMRRYPGRVPDVDLMFDCMDRPAINRTTGGPNPPLPPPLFRYCTTKDHLDIPFPDWSFWGWPETHINPWAKEFRAIKQGSRRVKWGDRVPLAFWKGNPDVASPLRLALLACNDTNLWHAQIMRQNWEEEAKSGYRHSALSTQCAHRYKVYAEGFAWSVSLKYILACGSMALVIDPRYEDFFSRGLEAKVNHWPVRADVGMCESIRDAVEWGNAHPEEAELVGRRGQRLMQELGMDAVYDYMLHLLTEYAKLLDFVPSPPDTAQEACVGSVLCLADEGQRRFLEMSKAEPATGEPCSLPPPPAAADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1HM14</accession>
    <name>I1HM14_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100845071</name>
        <name evidence="2" type="ORF">BRADI_2g36480v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231731.1">
        <property type="nucleotide sequence ID" value="XM_010233429.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HM14"/>
    <dbReference type="FunCoup" id="I1HM14">
        <property type="interactions" value="989"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HM14"/>
    <dbReference type="EnsemblPlants" id="KQK07595">
        <property type="protein sequence ID" value="KQK07595"/>
        <property type="gene ID" value="BRADI_2g36480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845071"/>
    <dbReference type="Gramene" id="KQK07595">
        <property type="protein sequence ID" value="KQK07595"/>
        <property type="gene ID" value="BRADI_2g36480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845071"/>
    <dbReference type="eggNOG" id="ENOG502QUNS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034794_1_0_1"/>
    <dbReference type="OMA" id="CKTAYLV"/>
    <dbReference type="OrthoDB" id="1918704at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35095">
        <property type="entry name" value="OS05G0143300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35095:SF1">
        <property type="entry name" value="OS05G0143300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK07595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="426" mass="45769" checksum="DB958401CF01BA08" modified="2012-06-13" version="1">MVGDLSLLAVASASPVLRAPSKELHGVLPFQGRGAASAAAPVSSASQLSPQQHILEGAYQAQQQPVHMMVPGLAQAAGAAYQAFDMAALIDVQDSHPDSVQLSLGIAEQCSRQEKILKFLMSGSDVKELDASILAELMGPQTLPINMSTQPYIPDDKLSIYEFGLEEPQQYLPENQLIIPDPLLEFAQSNGSALTIDENGRVVFPGNGDEMRDLLSVFLEFNVTKRESSGCKAAYLVPYFHRKRGRSQSNSASMAVDASKSTANVKAKSPLKKKQKGKNTNERDMYQNNYFHASEAILSILLDKDKSSSTILSLKKAGPEISELLTQCSIGIAGTGLAVLLSVVCKIATGGRTPFATTRLLNTSVGLGLFWLSSAVNGLRDLITSIFRGPSDTNLEDEVAVKIQRSTNDILFRAVTLLAITALKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSE9</accession>
    <name>I1HSE9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100826211</name>
        <name evidence="5" type="ORF">BRADI_2g52227v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564369.1">
        <property type="nucleotide sequence ID" value="XM_003564321.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSE9"/>
    <dbReference type="STRING" id="15368.I1HSE9"/>
    <dbReference type="EnsemblPlants" id="KQK10137">
        <property type="protein sequence ID" value="KQK10137"/>
        <property type="gene ID" value="BRADI_2g52227v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826211"/>
    <dbReference type="Gramene" id="KQK10137">
        <property type="protein sequence ID" value="KQK10137"/>
        <property type="gene ID" value="BRADI_2g52227v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826211"/>
    <dbReference type="eggNOG" id="ENOG502QSQ6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011924_2_0_1"/>
    <dbReference type="OMA" id="LSICARQ"/>
    <dbReference type="OrthoDB" id="642315at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="89"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat I (LRI)" evidence="3">
        <location>
            <begin position="385"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="464"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="545"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="685"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="3">
        <location>
            <begin position="495"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK10137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="765" mass="84151" checksum="7D146B6FCC3905C4" modified="2012-06-13" version="1">MDATPEEFLRTGGYLVDPEPLSPSLFLDLPPTPRADGPPAAAASAAADLDLDFISRMLMEEDIDDKFFYQYPDQPALLAAQRPYAQIISDTTSSGSPTGTTATSSDDSVINSNTATSSSDDTTNYTWPYDPIELSQLLRSPPYDGDMGFGGLVTDSPADEDTRSSFFPGDGAPTAEFQQSAALFKGAAEGGTTSFGQNGGSPGTQNSAILNDPAVEEEAKPAVFYDGDGDQGALVSAFFSGQTGSDMEMLNMAFLKGMEEANKFLPTNNTLPEVISDKPALRGFTVKKEELVDGTLTSGNGRGRRYRHDVDDLEAETGRNSKLMMPEHEETGAREMFDEIMLEGYEMCMKGMEDLRVAMDSEAKKNNTKGTGKAARAKRGTSEVVDLHTMLIHCAQAVAAGDRRSATELLKQIRQHSGPRGDATQRLAHCFAEGLEARLAGTGSQVYQSLVAKRTSVVEFLKAYKLFMAACCFKKVSFGFANLTILDAVVGKSRLHIVDFGVQYGLQWPGLMRLLAERDGGPPEVRITGIDLPQPGFRPACQIEETGRRLSNCAREFGVPFKFHSIAAKWETVRAEDLGIDRNEVLVVLCQCGLSNLMDESLVTDGLSPRDLVLRNIRNMRPDVFIQCVANGTYGAPFFVTRFREALFFYSAHFDMLDATIPRDNDERLLIERDIIGRAALNVIACEGADRVDRPETYKQWQVRNHRAGLRQLPLNPEIVKLAKEKVKNHYHKDFIIDVDHQWLLRGWKGRVLYAVSAWIAEDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUD5</accession>
    <name>I1HUD5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein terminal ear1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein LEAFY HEAD2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein PLASTOCHRON2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100835939</name>
        <name evidence="11" type="ORF">BRADI_2g58100v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Probable RNA-binding protein. Involved in the regular timing (plastochron) of lateral organs formation. May regulate the rate of leaf initiation and the duration of vegetative phase. Seems to be redundant to the function of PLASTOCHRON1, but to act in an independent pathway.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567374.3">
        <property type="nucleotide sequence ID" value="XM_003567326.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753738.1">
        <property type="nucleotide sequence ID" value="XM_014898252.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUD5"/>
    <dbReference type="FunCoup" id="I1HUD5">
        <property type="interactions" value="1246"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK11103">
        <property type="protein sequence ID" value="KQK11103"/>
        <property type="gene ID" value="BRADI_2g58100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835939"/>
    <dbReference type="Gramene" id="KQK11103">
        <property type="protein sequence ID" value="KQK11103"/>
        <property type="gene ID" value="BRADI_2g58100v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835939"/>
    <dbReference type="eggNOG" id="KOG4660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023146_1_0_1"/>
    <dbReference type="OMA" id="QELMPAP"/>
    <dbReference type="OrthoDB" id="417481at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12530">
        <property type="entry name" value="RRM3_EAR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000750">
        <property type="entry name" value="Protein terminal ear1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001402">
        <property type="entry name" value="Terminal EAR1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034458">
        <property type="entry name" value="EAR1-like_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="226"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="600"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="636"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="352"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="638"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="663"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00053062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00079602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK11103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="685" mass="73400" checksum="AE73CE9D8B50ED92" modified="2012-06-13" version="1">MEGRGAGMGGMPGAPPIILDAGAQEYYPAVVGGAAAYPPFLPLTPQQLYCPPPYQAMPPPPPPMALPLPLPMPMPIPIPQQIGPTAAAAAAAVDAPASRAVVLSLVPPHAQEGDVARAMAAFGAVRAVDASAVPSERVATVHFFDLRAAELAVAAVRAQHMRQQCRLSQLYAATVSWPPSAAGPGPAPAAWDWPHDDILGLVLGQAVWAQFAAASTLPDDGFSRGSLVVLNSLPDDVSLLELRQAFQAFGDLKDLRQSPHRPSHKFVEFFDTRDAARALAELNGQDFFGHRLVLEFTRPSTPGFRRRGYVLQQQPMAPIPPRLQQAWRPTFPQASSSSSGTGRGREGVVLMRRSSSAKSSGSGDRSKGGNNNNNNGAGRSHERKGKGGKKPTIVVVASSSASSSSTTEATTASSSGKQQCVKSVGRAGSGRSHRGWKGRFDKQFEFKEPEAAAADDTDTQEPETRTTVMIRNIPNKYSQKLVLNMLDAHCIVHNKKQIEAGESECQGQQQPLSSYDFLYLPIDFKNKCNVGYGFVNLTSPEAAVRLHKAFHQQPWEVFNSRKICQVTYARVQGLEALKQHFKNCSFPCESDEYLPVVFSPPRDGQQLTEPVPLVQPSLRAPTSNVDPLALDLMAAAPSSTSGDGASSTMSTHADEGHGASCSNDDDDDNDDEGLAEELQRLGYTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUR0</accession>
    <name>I1HUR0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100827968</name>
        <name evidence="8" type="ORF">BRADI_2g59240v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564965.1">
        <property type="nucleotide sequence ID" value="XM_003564917.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUR0"/>
    <dbReference type="STRING" id="15368.I1HUR0"/>
    <dbReference type="EnsemblPlants" id="KQK11288">
        <property type="protein sequence ID" value="KQK11288"/>
        <property type="gene ID" value="BRADI_2g59240v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73507">
        <property type="protein sequence ID" value="PNT73507"/>
        <property type="gene ID" value="BRADI_2g59240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827968"/>
    <dbReference type="Gramene" id="KQK11288">
        <property type="protein sequence ID" value="KQK11288"/>
        <property type="gene ID" value="BRADI_2g59240v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73507">
        <property type="protein sequence ID" value="PNT73507"/>
        <property type="gene ID" value="BRADI_2g59240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827968"/>
    <dbReference type="eggNOG" id="ENOG502QQMV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070441_1_0_1"/>
    <dbReference type="OMA" id="HPHHMWA"/>
    <dbReference type="OrthoDB" id="1911901at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017887">
        <property type="entry name" value="TF_TCP_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005333">
        <property type="entry name" value="Transcription_factor_TCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072:SF91">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03634">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51369">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TCP" evidence="7">
        <location>
            <begin position="88"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="270"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK11288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="319" mass="33465" checksum="8D71126312D1E8BC" modified="2012-06-13" version="1">MDPKFPPPPPLNKTEPTTGVTTTTTTTSQQQLDHEQYHQPQQHLQIQVHQQQQEEDGGGGKEQQQQVVAAAGAGERRVQGLGPKRSSNKDRHTKVDGRGRRIRMPALCAARIFQLTRELGHKSDGETVQWLLQQAEPAIVAATGSGTIPASALASVAPSLPSPTSALARPHHHHHLWGPSAAGFSPAGFMNSAPAGADSGGGLGGLMQRIGLPAGMELPGGGGGGHIGFAPMFASHAAAAAAMPGLELGLSQEGHIGVLAAQSFSQFYHQVGGAGGSGQLQHPHPQQHHHHQQPQQQQQQEDGEDERDEGDSDEESGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUV7</accession>
    <name>I1HUV7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100840298</name>
        <name evidence="14" type="ORF">BRADI_2g59710v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a cardiolipin + a 1-acyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:33731"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
            <dbReference type="ChEBI" id="CHEBI:64743"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33732"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:33733"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the taffazin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754612.1">
        <property type="nucleotide sequence ID" value="XM_014899126.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUV7"/>
    <dbReference type="STRING" id="15368.I1HUV7"/>
    <dbReference type="EnsemblPlants" id="KQK11365">
        <property type="protein sequence ID" value="KQK11365"/>
        <property type="gene ID" value="BRADI_2g59710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840298"/>
    <dbReference type="Gramene" id="KQK11365">
        <property type="protein sequence ID" value="KQK11365"/>
        <property type="gene ID" value="BRADI_2g59710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840298"/>
    <dbReference type="eggNOG" id="KOG2847">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031593_0_0_1"/>
    <dbReference type="OMA" id="PSAEICS"/>
    <dbReference type="OrthoDB" id="193467at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000872">
        <property type="entry name" value="Tafazzin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497:SF0">
        <property type="entry name" value="TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497">
        <property type="entry name" value="TAZ PROTEIN TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00979">
        <property type="entry name" value="TAFAZZIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="13">
        <location>
            <begin position="146"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK11365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="491" mass="54561" checksum="A88A053C96C44F91" modified="2012-06-13" version="1">MAAGGGIPWAERARAVGVQIRNRFRVAPVDRRWVWGRPDGRAASEAVRQWSDRIRDRLRRDRAPDQSSTEAASRPSSSALRFYRKKVGKVVDGAEDSVIIRSLQALAVPLIGNACHVFMHGLNSVQIYGAEKLEQALHGRPKGKPLLTVSNHVAAMDDPFVIASLLPPSVMMEAQKLRWTLCATDRCFTNPILSTFFRSVKVLPVSRGEGIYQKGMDMALSKLNNGGWVHIFPEGSRSRDGGKTVAPAKRGVGRLVMDADSLPVVIPFVHTGMQDIMPVGKRIPRTGKRVIVVVGDPIHFDDLIVDNGEDTQHISRGILYDKATERIGQQLQQLKVEVDRLAAEQKAELQSRCIDDTVNDGYRLWQQVDWEAFGIGNMLSSDREPLKQVQHELLLAEQSTSLPKQAEPELHLEEQSVSPVPGAAISPDVGVPHWFRRHSDPSELMGFAARGLLKNGRFMEEGYRQFPDSTALDDVWWGAQANNAMPRWSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1HWW9</accession>
    <name>I1HWW9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100832981</name>
        <name evidence="6" type="ORF">BRADI_3g03010v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin T subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233768.2">
        <property type="nucleotide sequence ID" value="XM_010235466.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HWW9"/>
    <dbReference type="STRING" id="15368.I1HWW9"/>
    <dbReference type="EnsemblPlants" id="KQJ93161">
        <property type="protein sequence ID" value="KQJ93161"/>
        <property type="gene ID" value="BRADI_3g03010v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832981"/>
    <dbReference type="Gramene" id="KQJ93161">
        <property type="protein sequence ID" value="KQJ93161"/>
        <property type="gene ID" value="BRADI_3g03010v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832981"/>
    <dbReference type="eggNOG" id="KOG0834">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022000_8_2_1"/>
    <dbReference type="OMA" id="NGSDARF"/>
    <dbReference type="OrthoDB" id="10264655at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HWW9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008024">
        <property type="term" value="C:cyclin/CDK positive transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032786">
        <property type="term" value="P:positive regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000081">
        <property type="entry name" value="Cyclin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="51"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="166"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="391"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="421"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="447"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="487"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="503"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="554"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00061204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ93161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="616" mass="68136" checksum="CC4297F834BBD006" modified="2012-06-13" version="1">MAPAAAAGEEGGGGRGRSWYLTKEEVEAGSPSRKHGVTKDKEDRIRGIYCSFMRDVGGVLKLPQITVATAIMLCHRFYLLQSHVKNEWQTIATACILLASKIEDTPCSLKRVVIAAYETMYRRKPDTARRIHEKEFLEKRKSLVVVGERLLLSTIRFDFNIQHPYGPLNCALENLGISQKEVQQAAVNLIHDALRSTLVVQFKPHYIAAASLFLAAKREGFKLPLGKGKVWWQQFDVAPQQLEAAVSQMREVCVKRKPGPTVPAVRPTPDPTPVEKQPEMNFPKPVLKYVYSRRPQSRPAPAGTPTLVEKQQIISTVDPVLRPTQSSGGDLSRPTAVPTATPTLVEKEQIIRSVGAVLKPTNSSGGGPSEPTAVPTATPDVVEKQQIISTPDSVLRHADPSRGGLSRHTTAPTVTPETSTLAKKQQKISTPDSVLRHTHPSKGGLSRPTTSATRDSTLVKKQQVVSTLESVQSHAHPSRRGLTSNNFDREAPRRMGVDRLVHHNSTGSSVRNGSNKPLPRNEDNKPLRRHMDLGSNRTVRDERSEKQSSQSALKADHVHGEQKDIDVARVRKRRIQEDGEHPTPVDRSDQDSWTGQHIQSVMVVEPKLPSLKRHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1I0U3</accession>
    <name>I1I0U3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Knottin scorpion toxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g14700v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I0U3"/>
    <dbReference type="EnsemblPlants" id="KQJ95019">
        <property type="protein sequence ID" value="KQJ95019"/>
        <property type="gene ID" value="BRADI_3g14700v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95019">
        <property type="protein sequence ID" value="KQJ95019"/>
        <property type="gene ID" value="BRADI_3g14700v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R4YF">
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    </dbReference>
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    <dbReference type="InParanoid" id="I1I0U3"/>
    <dbReference type="OMA" id="GAICECY"/>
    <dbReference type="OrthoDB" id="706664at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094941" description="Knottin scorpion toxin-like domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ95019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9195" checksum="D7A4959F326BB0FE" modified="2012-06-13" version="1">MEAWRKTTVGFLVLILVAASSYEVLSVENNCFWKFSSRHCVNSPACRDACIERGADDGQCRNRYKKITGAICECYPKECNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1D2</accession>
    <name>I1I1D2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="13">CslF6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100834026</name>
        <name evidence="14" type="ORF">BRADI_3g16307v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2015" name="Sci. Adv." volume="1" first="e1500069" last="e1500069">
            <title>Membrane pore architecture of the CslF6 protein controls (1-3,1-4)-?-glucan structure.</title>
            <authorList>
                <person name="Jobling S.A."/>
            </authorList>
            <dbReference type="PubMed" id="26601199"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KP260637">
        <property type="protein sequence ID" value="AKJ66173.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573454.1">
        <property type="nucleotide sequence ID" value="XM_003573406.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1D2"/>
    <dbReference type="FunCoup" id="I1I1D2">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I1D2"/>
    <dbReference type="EnsemblPlants" id="KQJ95295">
        <property type="protein sequence ID" value="KQJ95295"/>
        <property type="gene ID" value="BRADI_3g16307v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834026"/>
    <dbReference type="Gramene" id="KQJ95295">
        <property type="protein sequence ID" value="KQJ95295"/>
        <property type="gene ID" value="BRADI_3g16307v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834026"/>
    <dbReference type="eggNOG" id="ENOG502QU14">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001418_3_1_1"/>
    <dbReference type="OMA" id="WIEPSEN"/>
    <dbReference type="OrthoDB" id="72851at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009833">
        <property type="term" value="P:plant-type primary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000027">
        <property type="entry name" value="Cellulose synthase-like protein D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="705"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="737"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="772"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="827"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="856"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="890"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="628"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AKJ66173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ95295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="939" mass="104272" checksum="D91F704E70087319" modified="2012-06-13" version="1">MAPAVAGGSSRGAGCKCGFQVCVCSGSAAVASAGSSLEVERAMAVTPVEGQAAPVDGESWVGVELGPDGVETDESGAGVDDRPVFKTEKIKGVLLHPYRVLIFVRLIAFTLFVIWRISHKNPDTMWLWVTSICGEFWFGFSWLLDQLPKLNPINRIPDLAVLRQRFDRADGTSTLPGLDIFVTTADPIKEPILSTANSVLSILAADYPVDRNTCYISDDSGMLMTYEAMAESAKFATLWVPFCRKHGIEPRGPESYFELKSHPYMGRAHDEFVNDRRRVRKEYDDFKAKINSLETDIQQRNDLHNAAVPQNGDGIPRPTWMADGVQWQGTWVEPSANHRKGDHAGIVLVLIDHPSHDRLPGAPASADNALDFSGVDTRLPMLVYMSREKRPGHNHQKKAGAMNALTRASALLSNAPFILNLDCDHYINNSQALRAGICFMVGRDSDTVAFVQFPQRFEGVDPTDLYANHNRIFFDGTLRALDGMQGPIYVGTGCLFRRITVYGFDPPRINVGGPCFPALGGLFAKTKYEKPSMEMTMARANQAVVPAMAKGKHGFLPLPKKTYGKSDKFVDTIPRASHPSPYAAEGIRVVDSGAETLAEAVKVTGSAFEQKTGWGSELGWVYDTVTEDVVTGYRMHIKGWRSRYCSIYPHAFIGTAPINLTERLFQVLRWSTGSLEIFFSKNNPLFGSTYLHPLQRVAYINITTYPFTAIFLIFYTTVPALSFVTGHFIVQRPTTMFYVYLGIVLATLLIIAVLEVKWAGVTVFEWFRNGQFWMTASCSAYLAAVCQVLTKVIFRRDISFKLTSKLPAGDEKKDPYADLYVVRWTPLMITPIIIIFVNIIGSAVAFAKVLDGEWTHWLKVAGGVFFNFWVLFHLYPFAKGLLGKHGKTPVVVLVWWAFTFVITAVLYINIPHIHGGGGKHSVGHGMHHGKKFDGYYLWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2I4</accession>
    <name>I1I2I4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_3g19600v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2I4"/>
    <dbReference type="EnsemblPlants" id="KQJ95885">
        <property type="protein sequence ID" value="KQJ95885"/>
        <property type="gene ID" value="BRADI_3g19600v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95885">
        <property type="protein sequence ID" value="KQJ95885"/>
        <property type="gene ID" value="BRADI_3g19600v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1278">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1231403_0_0_1"/>
    <dbReference type="InParanoid" id="I1I2I4"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF154">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ95885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="225" mass="25330" checksum="24653EFB854A5466" modified="2012-06-13" version="1">MAPEKRAFFDQLRLRHAEPSITRSSRRTRSQRNFLRCVPTTSHAFRCGLLQGGDKEEGGLSIPSSDDGKHVAGPPSICSYSARARCLVHVPYYNIGVHCYGCPNDMKHPNKADAYTGGHEKAKETRIRRMFADQRSCKRSRYATVSTTCGVGGHTLTTGSSALYLFLYATFYFFTKLEITKFVSDVLYFGYMLISSYAFFALTGTIGFYACFMFTRLIYSSVKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2S3</accession>
    <name>I1I2S3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100825830</name>
        <name evidence="3" type="ORF">BRADI_3g20490v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571630.1">
        <property type="nucleotide sequence ID" value="XM_003571582.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2S3"/>
    <dbReference type="STRING" id="15368.I1I2S3"/>
    <dbReference type="EnsemblPlants" id="KQJ96026">
        <property type="protein sequence ID" value="KQJ96026"/>
        <property type="gene ID" value="BRADI_3g20490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825830"/>
    <dbReference type="Gramene" id="KQJ96026">
        <property type="protein sequence ID" value="KQJ96026"/>
        <property type="gene ID" value="BRADI_3g20490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825830"/>
    <dbReference type="eggNOG" id="ENOG502QRNX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057230_1_1_1"/>
    <dbReference type="OMA" id="PTWPRGV"/>
    <dbReference type="OrthoDB" id="440455at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14279">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31245:SF3">
        <property type="entry name" value="OS08G0313600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31245">
        <property type="entry name" value="UBIQUITIN SYSTEM COMPONENT CUE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="48"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ96026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="277" mass="30535" checksum="55289359114E2B0B" modified="2012-06-13" version="1">MSAVVCGKRASSIFEELSNGSGSPPAAKRARFFGSASGPLPAWPRAAADPALVADLSARFPAMSIQFIEKALEESGNDLDSAIKSLLNLQLDPVENIGDHACERPNQIINEVQASVQGLSDGDRVTAPSECAPSSANLMSDGSGWVEYFTNQMATAGNIDEARVRAARALEAFQKDVIARSNAQAPHEIQKENIALKVQLESLIKENTILRKLFTKQHERQKDYDEKNQELQQMKQHIAQYQERIRTLEVNNYALSMHLRQAQQSSSIPGRHHPDVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1I703</accession>
    <name>I1I703_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100839728</name>
        <name evidence="10" type="ORF">BRADI_3g35732v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574476.1">
        <property type="nucleotide sequence ID" value="XM_003574428.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235120.1">
        <property type="nucleotide sequence ID" value="XM_010236818.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I703"/>
    <dbReference type="STRING" id="15368.I1I703"/>
    <dbReference type="EnsemblPlants" id="KQJ98251">
        <property type="protein sequence ID" value="KQJ98251"/>
        <property type="gene ID" value="BRADI_3g35732v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839728"/>
    <dbReference type="Gramene" id="KQJ98251">
        <property type="protein sequence ID" value="KQJ98251"/>
        <property type="gene ID" value="BRADI_3g35732v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839728"/>
    <dbReference type="eggNOG" id="KOG1590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099502_5_1_1"/>
    <dbReference type="OMA" id="FLQHFWG"/>
    <dbReference type="OrthoDB" id="1697690at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050833">
        <property type="term" value="F:pyruvate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ98251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="107" mass="11854" checksum="4B8941FD06AF6474" modified="2012-06-13" version="1">MATAFKAFVNSPVGPKTTHFWGPVANWGFVLAGLVDLNKPPEMISGNMTAAMCVYSGLFMRFAWMVQPRNYLLLACHASNETVQLYHLSRCAKAQGYIGKKEPEAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8F2</accession>
    <name>I1I8F2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dilute domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100845006</name>
        <name evidence="3" type="ORF">BRADI_3g39880v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574740.1">
        <property type="nucleotide sequence ID" value="XM_003574692.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8F2"/>
    <dbReference type="STRING" id="15368.I1I8F2"/>
    <dbReference type="EnsemblPlants" id="KQJ98902">
        <property type="protein sequence ID" value="KQJ98902"/>
        <property type="gene ID" value="BRADI_3g39880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845006"/>
    <dbReference type="Gramene" id="KQJ98902">
        <property type="protein sequence ID" value="KQJ98902"/>
        <property type="gene ID" value="BRADI_3g39880v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845006"/>
    <dbReference type="eggNOG" id="ENOG502QQNG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013939_2_0_1"/>
    <dbReference type="OMA" id="IAHFTAQ"/>
    <dbReference type="OrthoDB" id="20172at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF25">
        <property type="entry name" value="OS08G0505200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="630"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ98902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="78089" checksum="F076BE33A43C551F" modified="2012-06-13" version="1">MGAKENGEVRDEKGAGPDYEPARVSRSSQGEAHGNEDNRVRRVSGVPKKLVKKETKESSPRMAKSNTGRQVQNKLQHKALSDTQNRSPKPRKAVNAAKAIEVRRPDVVKIPSRSPSEFSEETDDIISEAGTTDDRGIEEAKEMDVLDEAPHCDQSTGTDDDIVDIEEKIIDDEKTVLHQRNEELQSKLEKLEQELREVAALEVSLYSVMPEHGSSAHKLHTPARRLSRLYIHASKFWPIDKTASVAKNTVSGLVLVAKSCSNDASRLTFWLSNTVVLREIIAKSFGISRQSTPTMKTKNINDSAKWFDGKSMPMLQNNNSNGKQTNLAAIQILDDWQETSTLLAALEKIESWIFSRIVETVWWQALTPHMQTRAEGSSTPKAGKVLGPSLGDQQQGTFSVNLWKAAFHDAFSRICPLRAGGHECGCLPVLAKLVMEQCVARLDVAMFNAILRESASEIPTDPISDPIVDPKVLPIPAGELSFGSGAQLKNSIGNWSRWLTDNLGIDDDDSEDECLDIGNGNDERSGAAEGKSFRLLNELSDLLMLPKDMLLENSIRKEICPSVGLPLVTRILCNFTPDEFCPDPVPSIVLEELNSESLLERSIGKDAASAFPCIAAPVTYRPPSQLDVAEKVSDTGGGAKLDRRGSMVQRRGYTSDDDLDDLDSPLASLFDKSAPPSPSNGVTPFTAQLRGASMENARYTLLREVWSERH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8X4</accession>
    <name>I1I8X4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic</fullName>
            <ecNumber evidence="7">5.4.3.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100845106</name>
        <name evidence="13" type="ORF">BRADI_3g41270v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="7"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574826.1">
        <property type="nucleotide sequence ID" value="XM_003574778.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8X4"/>
    <dbReference type="FunCoup" id="I1I8X4">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I8X4"/>
    <dbReference type="EnsemblPlants" id="KQJ99122">
        <property type="protein sequence ID" value="KQJ99122"/>
        <property type="gene ID" value="BRADI_3g41270v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845106"/>
    <dbReference type="Gramene" id="KQJ99122">
        <property type="protein sequence ID" value="KQJ99122"/>
        <property type="gene ID" value="BRADI_3g41270v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845106"/>
    <dbReference type="eggNOG" id="KOG1401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_1"/>
    <dbReference type="OMA" id="WGPLIFG"/>
    <dbReference type="OrthoDB" id="425114at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00668"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000021">
        <property type="entry name" value="Glutamate-1-semialdehyde 2,1-aminomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000012">
        <property type="entry name" value="Glutamate-1-semialdehyde 2,1-aminomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000818">
        <property type="entry name" value="PRK00062.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713:SF3">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ99122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="472" mass="49578" checksum="CAF1FA77C4278989" modified="2012-06-13" version="1">MAGAAAAAVASGISIRPAVAPKSSLAPRGRSMVRAAVSVEKGASAYTVQKSEEIFNAAKELMPGGVNSPVRAFKSVGGQPIVFDSVKGSHMWDVDGNEYIDYVGSWGPAIIGHADDTVNAALIETLKKGTSFGAPCALENVLAQMVISAVPSIEMVRFVNSGTEACMGALRLVRAFTGREKILKFEGCYHGHADSFLVKAGSGVATLGLPDSPGVPKGATVGTLTAPYNDAEAVKKLFEDNKGEIAAVFLEPVVGNAGFIPPSPAFLTALRELTKQDGALLVFDEVMTGFRLAYGGAQEYFGITPDVTTLGKIIGGGLPVGAYGGRKDIMEMVAPAGPMYQAGTLSGNPLAMTAGIHTLKRLMEPGTYEYLDKITGELVRGILDSGTKTGHEMCGGHIRGMFGFFFAGGPVNNFDDAKKSDTAKFGRFHRGMLEEGVYLAPSQFEAGFTSLAHTPQDIEKTVEAAEKVLRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1I9I1</accession>
    <name>I1I9I1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA primase</fullName>
            <ecNumber evidence="12">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100835644</name>
        <name evidence="13" type="ORF">BRADI_3g42937v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="12"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572504.1">
        <property type="nucleotide sequence ID" value="XM_003572456.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I9I1"/>
    <dbReference type="STRING" id="15368.I1I9I1"/>
    <dbReference type="EnsemblPlants" id="KQJ99380">
        <property type="protein sequence ID" value="KQJ99380"/>
        <property type="gene ID" value="BRADI_3g42937v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835644"/>
    <dbReference type="Gramene" id="KQJ99380">
        <property type="protein sequence ID" value="KQJ99380"/>
        <property type="gene ID" value="BRADI_3g42937v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835644"/>
    <dbReference type="eggNOG" id="KOG2851">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028288_3_2_1"/>
    <dbReference type="OMA" id="NVTRGFN"/>
    <dbReference type="OrthoDB" id="19606at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I9I1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.920.10:FF:000001">
        <property type="entry name" value="DNA primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5 12" id="KW-0639">Primosome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ99380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="450" mass="51618" checksum="27F2CE978EEE623B" modified="2012-06-13" version="1">MEDERAMEIDGQQQQREVAAAVPEGFNADYLRIYYGKLFPYGDFFKWLSYGNDAKHPGCDQSYIGRRELSFTLENDIYLRFQSFDSAAELESSIKEKCPFKIDIGPVYSVDPAKRHAYAQSGNNVFAPVERELIFDIDISDYDDVRYCCSGADTCVDCWPLMTIVIKILDTSLRGDFGFNHILWVYSGRRGVHCWVCDSRARKLSNEQRSAIADYFRVYKGGENTSKKVSLTGPVLHPFLARSYTDVLKCFFEDKLLHSQQLFSSEERCNKILELIPDENVASELHDKWQGNRRSSISKEDVNAARWEQLKTTLQSGKHKAQGLRRCVEEIVFSYTYPRLDMEVSKHMNHLLKAPFCIHPKTGRVCVPIDPNNCDDFDPAAVPTLSQLLGELNAAGMQIDSESDWERTSLEKSIRFFRTSFLQPMLKACKVELENAYNAKLEQSKNALNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I1IA31</accession>
    <name>I1IA31_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g44500v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IA31"/>
    <dbReference type="EnsemblPlants" id="KQJ99652">
        <property type="protein sequence ID" value="KQJ99652"/>
        <property type="gene ID" value="BRADI_3g44500v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99652">
        <property type="protein sequence ID" value="KQJ99652"/>
        <property type="gene ID" value="BRADI_3g44500v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1837864_0_0_1"/>
    <dbReference type="InParanoid" id="I1IA31"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ99652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="140" mass="16358" checksum="7166F31948A1A2DF" modified="2012-06-13" version="1">MASSLPWRPLRIPFPWLRIPFPWLSSLSLMWFLSLLLNPRTTTCTLQQSRLEKGMCFLKFTAKVPVCNKNPYFQLRIAIRVIVKHPDKWYSTQNTMVAEQFCMKQVNEIFSVMGMHETMCQLCSQSIVAQKCRSIPNLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IA83</accession>
    <name>I1IA83_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">N-carbamoylputrescine amidase</fullName>
            <ecNumber evidence="5">3.5.1.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100821425</name>
        <name evidence="8" type="ORF">BRADI_3g44960v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from N-carbamoylputrescine (amidase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the carbon-nitrogen hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.53" evidence="5"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575077.1">
        <property type="nucleotide sequence ID" value="XM_003575029.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IA83"/>
    <dbReference type="STRING" id="15368.I1IA83"/>
    <dbReference type="EnsemblPlants" id="KQJ99737">
        <property type="protein sequence ID" value="KQJ99737"/>
        <property type="gene ID" value="BRADI_3g44960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821425"/>
    <dbReference type="Gramene" id="KQJ99737">
        <property type="protein sequence ID" value="KQJ99737"/>
        <property type="gene ID" value="BRADI_3g44960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821425"/>
    <dbReference type="eggNOG" id="KOG0806">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_4_0_1"/>
    <dbReference type="OMA" id="APYFCQV"/>
    <dbReference type="OrthoDB" id="412018at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050126">
        <property type="term" value="F:N-carbamoylputrescine amidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033388">
        <property type="term" value="P:putrescine biosynthetic process from arginine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07573">
        <property type="entry name" value="CPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000012">
        <property type="entry name" value="N-carbamoylputrescine amidohydrolase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017755">
        <property type="entry name" value="N-carbamoylputrescine_amidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03381">
        <property type="entry name" value="agmatine_aguB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF2">
        <property type="entry name" value="BETA-UREIDOPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="7">
        <location>
            <begin position="11"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ99737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="302" mass="33640" checksum="FFE60C6EB8A7FF0C" modified="2012-06-13" version="1">MAGGGGAGRKVAVAAVQFACTDTESDNVAAAERLIREAHKKGANIVLIQELFEGHYFCQAQRMDFFRRAKPYKENPTILRMQKLAKELDVVIPVSFFEEANNAHYNSVAIIDADGTDLGLYRKSHIPDGPGYQEKFYFNPGDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDINLDSREHWKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDIDEEVLVAEFDLDEIKSTRHGWGIFRDRRIDLYKVLLTLDGEKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IB58</accession>
    <name>I1IB58_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="13">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100827804</name>
        <name evidence="15" type="ORF">BRADI_3g47590v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Probable methyltransferase required to silence rDNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
            <location evidence="1 13">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="13"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235629.1">
        <property type="nucleotide sequence ID" value="XM_010237327.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IB58"/>
    <dbReference type="FunCoup" id="I1IB58">
        <property type="interactions" value="665"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IB58"/>
    <dbReference type="EnsemblPlants" id="KQK00144">
        <property type="protein sequence ID" value="KQK00144"/>
        <property type="gene ID" value="BRADI_3g47590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827804"/>
    <dbReference type="Gramene" id="KQK00144">
        <property type="protein sequence ID" value="KQK00144"/>
        <property type="gene ID" value="BRADI_3g47590v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3045">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="HEGFRAQ"/>
    <dbReference type="OrthoDB" id="10258825at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IB58">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2150:FF:000001">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000068">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 13" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK00144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="298" mass="32598" checksum="2AC8F85DBC3DBEC8" modified="2012-06-13" version="1">MEQDSAAVGRKRRRRGGAANRRKTSSSSQEACPAAPQAAAPSSEKRRRKAGEVAAAPKAKGRKTTSLLDKMRARLSGGHFRMLNEKLYTCSGEDAFEYFKNEPELFDVYHAGYQEQMSRWPEQPVNVIINWLKSHNKSWTVADFGCGSAAVAKNVKNKVFSIDLVSDDPSVIACDMAHTPLEPSSVDVAIFCLSLMGTNFPSYLQEANRVLKPSGWLLIAEVRSRLDPNTGGADPDKFSEAISQLGFSLVSKAYFMSTAKSALCFLIAIRLLSAGCEQQNVCPVLFQEKGKEQGGGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBI3</accession>
    <name>I1IBI3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100835125</name>
        <name evidence="3" type="ORF">BRADI_3g48567v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572709.1">
        <property type="nucleotide sequence ID" value="XM_003572661.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBI3"/>
    <dbReference type="STRING" id="15368.I1IBI3"/>
    <dbReference type="EnsemblPlants" id="KQK00308">
        <property type="protein sequence ID" value="KQK00308"/>
        <property type="gene ID" value="BRADI_3g48567v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835125"/>
    <dbReference type="Gramene" id="KQK00308">
        <property type="protein sequence ID" value="KQK00308"/>
        <property type="gene ID" value="BRADI_3g48567v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835125"/>
    <dbReference type="eggNOG" id="KOG2714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045194_1_0_1"/>
    <dbReference type="OMA" id="NDICWID"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18316">
        <property type="entry name" value="BTB_POZ_KCTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057441">
        <property type="entry name" value="Beta_prop_At2g24240"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF77">
        <property type="entry name" value="OS02G0612700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25279">
        <property type="entry name" value="Beta_prop_At2g24240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="11"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK00308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="468" mass="49991" checksum="192CF2D60394E835" modified="2012-06-13" version="1">MCTPAAAAGRGRVRLNVGGKVFETTAATLSSAGRDTMLGAMVDASWNAGPAAAAEYFIDRDPACFAVLLDLLRTGALSVPPHVSERALCREALYYGLLDRVRAARLEDGDGFDGDRLRLAASVPGPGRAPGHDGTAVRAAPDGGCCVAHGGALVRVYNWMLEERPPVHLDHAPVNDAAYLDASTLLLAARERPGSGGRRDGHHGNGGLAVFSALTGEIRHRFRVTHDKQPRPFTPGALAFDDRQNSKIFASCKGRLNEYGVGAWDATTGQQTDFFYEPPGCALGDADKLQWLEGTSTLMAATMFPRTDSSFISVLDFRAKDVAWSWSDVGTPASLEDKHAVHAVAMDDGRSVCVVNQYDDLGFLDLRSNAGGVRWRSRSKLLRKTKKAMHAEETCYPKLAAHGGQLFASTDDAVSVFSGPDHALTSTLRGSDGGGAICDFSIGGGRLFALHNEENVFDVWETPPPPII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBJ5</accession>
    <name>I1IBJ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100824824</name>
        <name evidence="1" type="ORF">BRADI_3g48660v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235695.1">
        <property type="nucleotide sequence ID" value="XM_010237393.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBJ5"/>
    <dbReference type="STRING" id="15368.I1IBJ5"/>
    <dbReference type="EnsemblPlants" id="KQK00323">
        <property type="protein sequence ID" value="KQK00323"/>
        <property type="gene ID" value="BRADI_3g48660v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00324">
        <property type="protein sequence ID" value="KQK00324"/>
        <property type="gene ID" value="BRADI_3g48660v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69054">
        <property type="protein sequence ID" value="PNT69054"/>
        <property type="gene ID" value="BRADI_3g48660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824824"/>
    <dbReference type="Gramene" id="KQK00323">
        <property type="protein sequence ID" value="KQK00323"/>
        <property type="gene ID" value="BRADI_3g48660v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00324">
        <property type="protein sequence ID" value="KQK00324"/>
        <property type="gene ID" value="BRADI_3g48660v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69054">
        <property type="protein sequence ID" value="PNT69054"/>
        <property type="gene ID" value="BRADI_3g48660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824824"/>
    <dbReference type="eggNOG" id="KOG3487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085828_0_2_1"/>
    <dbReference type="OMA" id="FFQELHE"/>
    <dbReference type="OrthoDB" id="10252102at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IBJ5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14825">
        <property type="entry name" value="TRAPPC2_sedlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT69054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15367" checksum="9F18F019414E9A8D" modified="2012-06-13" version="1">MASTACFVIVSKNDIPIYDAEVGSAPKKEDQAYQHQFILHAALDVVQDLAWTTNAMSLKSVDRFNELVVSVYVTAGHTRFMLLHDTRTEDGIKSFFQEVHELYIKIFLNPLYLPGSRITSSHFDTKVRALARKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDA9</accession>
    <name>I1IDA9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100821020</name>
        <name evidence="6" type="ORF">BRADI_3g53630v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572883.1">
        <property type="nucleotide sequence ID" value="XM_003572835.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDA9"/>
    <dbReference type="STRING" id="15368.I1IDA9"/>
    <dbReference type="EnsemblPlants" id="KQK01063">
        <property type="protein sequence ID" value="KQK01063"/>
        <property type="gene ID" value="BRADI_3g53630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821020"/>
    <dbReference type="Gramene" id="KQK01063">
        <property type="protein sequence ID" value="KQK01063"/>
        <property type="gene ID" value="BRADI_3g53630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821020"/>
    <dbReference type="eggNOG" id="KOG3017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035730_8_1_1"/>
    <dbReference type="OMA" id="ERDEYNF"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05381">
        <property type="entry name" value="CAP_PR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000006">
        <property type="entry name" value="Putative pathogenesis-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095210" description="SCP domain-containing protein" evidence="4">
        <location>
            <begin position="33"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="5">
        <location>
            <begin position="42"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK01063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="180" mass="19452" checksum="FEA124755FA9E4E3" modified="2012-06-13" version="1">MASSTCPRAHLAIALFVTGLLFSSNAITVVDAYPRNGGGGGDLRPQFLYPQNAARAAMGLPPLRWDEGVASYARSYAESRRGDCALVHSSGPYGENLFWGSGGDGGWTPAQAVGAWLAERPRYDYWSNRCSGGMCGHYTQIVWRGSTRVGCAMVNCYNGRGTFITCNYDPPGNYVGMRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDJ0</accession>
    <name>I1IDJ0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100835228</name>
        <name evidence="14" type="ORF">BRADI_3g54290v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570321.1">
        <property type="nucleotide sequence ID" value="XM_003570273.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDJ0"/>
    <dbReference type="FunCoup" id="I1IDJ0">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01180">
        <property type="protein sequence ID" value="KQK01180"/>
        <property type="gene ID" value="BRADI_3g54290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835228"/>
    <dbReference type="Gramene" id="KQK01180">
        <property type="protein sequence ID" value="KQK01180"/>
        <property type="gene ID" value="BRADI_3g54290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835228"/>
    <dbReference type="eggNOG" id="KOG0698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013173_21_1_1"/>
    <dbReference type="OMA" id="TAMGESW"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IDJ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000004">
        <property type="entry name" value="Probable protein phosphatase 2C 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF684">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C 27-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="13">
        <location>
            <begin position="60"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK01180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="360" mass="40200" checksum="983CA1D788DF7C87" modified="2012-06-13" version="1">MCVEGFEDAERLDFGAAEIERPADFLMERVCENMVSLDFKQTKMVSGTGESWHNFVPAIRSGEWSDIGGRDYMEDAHVCISNLVKNFGCNKADDEIISFYGVFDGHGGKDAAHYVRDNLPRIIVEDADFPLELEKVVRRSFVQTDSQFAEKCSRHDALSSGTTALTAMIFGRSLLVANAGDCRAVLSRRGAAIEMSKDHRTCCLNERKRVESLGGYVDDGYLNGQLAVTRALGDWHLDGLKEMGEPGGPLSAEPELKMITLTKEDEFLLIGSDGIWDYFSNQNSVDFARRRLQEHNDLRLCCKEIIEEAIRRGATDNLTAVMVSFHQEAPPQIRVNRTGRVERTISAEGLHSLRVLLEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IF08</accession>
    <name>I1IF08_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100844180</name>
        <name evidence="5" type="ORF">BRADI_3g58600v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570602.1">
        <property type="nucleotide sequence ID" value="XM_003570554.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IF08"/>
    <dbReference type="FunCoup" id="I1IF08">
        <property type="interactions" value="2458"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IF08"/>
    <dbReference type="EnsemblPlants" id="KQK01815">
        <property type="protein sequence ID" value="KQK01815"/>
        <property type="gene ID" value="BRADI_3g58600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844180"/>
    <dbReference type="Gramene" id="KQK01815">
        <property type="protein sequence ID" value="KQK01815"/>
        <property type="gene ID" value="BRADI_3g58600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844180"/>
    <dbReference type="eggNOG" id="KOG4209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_23_4_1"/>
    <dbReference type="OMA" id="SSCCANA"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12306">
        <property type="entry name" value="RRM_II_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF22">
        <property type="entry name" value="OS02G0757900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="84"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK01815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="210" mass="23859" checksum="DC937174F1094412" modified="2012-06-13" version="1">MDEEEHEVYGQEIPVDGDMEGGDVDMTTGGDDAAKELDEMKRRLKEMEEEAAALRDMQAKVAKEMQGGDANASTAEAKEQVDARSVYVGNVDYACTPEEVQQHFQACGTVNRVTILTDKFGQPKGFAYVEFLEQEAVQEALNLNESELHGRQIKVSPKRTNVPGMKQRPPRGFNPYHGYPYRSYGAQYFPPYGYGRVPRFRRPTRYRPYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IF18</accession>
    <name>I1IF18_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100846612</name>
        <name evidence="6" type="ORF">BRADI_3g58700v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570610.1">
        <property type="nucleotide sequence ID" value="XM_003570562.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IF18"/>
    <dbReference type="FunCoup" id="I1IF18">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IF18"/>
    <dbReference type="EnsemblPlants" id="KQK01833">
        <property type="protein sequence ID" value="KQK01833"/>
        <property type="gene ID" value="BRADI_3g58700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846612"/>
    <dbReference type="Gramene" id="KQK01833">
        <property type="protein sequence ID" value="KQK01833"/>
        <property type="gene ID" value="BRADI_3g58700v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846612"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001724_0_0_1"/>
    <dbReference type="OMA" id="ADVPMVC"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000027">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000055">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1459">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="5">
        <location>
            <begin position="10"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK01833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="486" mass="53273" checksum="F5010E71BA2FC021" modified="2012-06-13" version="1">MSSSSRPHAVLIPYPAQGHVTPLLHLAKVLHARGFYITFVNSEYNHRRLVRSRGAASLSLPATDGFRFETMPDGLPPCDNEDVTQDIPTLCTSLSTHGADLLRHLLARLVNDGETPPVTCLIPDGVMSFALDVAEEMRVPALVFWTTSACGFMGYLHFAELIERGIVPLKDESCLSNGYLDTELDWVPGMPGIRLRDMPSFVRTTDKDDVMLNFDSREAQNAYRAQGVILNTFHAVEEDVVNAFRGIFPQGVYAVGPLQAFAASASLAHPELATIGGNLWTEDISCLTWLDTKETGSVVYVNFGSITVMSPGHLAEFAWGLARCGRPFLWVIRPDLVAGEKAVLPEDFVSETKGRGMFASWCPQEEVLRHPATGLFLTHSGWNSTLESICAGVPMVCWPFFAEQMTNCRYACTTWGIGMEIGSDVRREEVARLVGEAMDGDRGKEMRAMAEMWKEKSVAATEDGGTSSVDIVRLVEFLLAGCVPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1ILS2</accession>
    <name>I1ILS2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ELMO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100822997</name>
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        <name type="scientific">Brachypodium distachyon</name>
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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    </organism>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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            <strain evidence="2">Bd21</strain>
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    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_4g19140v3"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="103"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ88531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="282" mass="32259" checksum="3AAF3CC01B113D4F" modified="2012-06-13" version="1">MEAARPAETTTQHHGGGCLAAARTFMPPAGPVARCSGGSSLAGSSDEASCGSPKWIEKGLSCVCIKRRGAYERICMNLTPLQEQRLQRLRHRMKVYFDPSRRNHQEALRALWYATYPDQELQGLISEQWKDMGWQGRDPSTDFRGAGFISLENLLFFAKTFSASFQRLLKKQSGNRATWEYPFAVAGVNITFMIMQMLDLQSTKPRTFVRAVFIQMLSEDEWAFDLLYCVAFLVMDKQWLEKNASYMDFNEILKSTRTQLERELLLEDVMRIEDMPSYGLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQW5</accession>
    <name>I1IQW5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100830057</name>
        <name evidence="8" type="ORF">BRADI_4g32620v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the purine permeases (TC 2.A.7.14) family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003578215.4"/>
    </dbReference>
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        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IQW5"/>
    <dbReference type="EnsemblPlants" id="KQJ90579">
        <property type="protein sequence ID" value="KQJ90579"/>
        <property type="gene ID" value="BRADI_4g32620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830057"/>
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        <property type="gene ID" value="BRADI_4g32620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830057"/>
    <dbReference type="eggNOG" id="ENOG502QTN9">
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015211">
        <property type="term" value="F:purine nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030182">
        <property type="entry name" value="PUP_plant"/>
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        <property type="entry name" value="OS09G0467300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376:SF99">
        <property type="entry name" value="PURINE PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16913">
        <property type="entry name" value="PUNUT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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    </feature>
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            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006213"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="397" mass="42412" checksum="0E282E455480F5FA" modified="2012-06-13" version="1">MEVETPPAVLSRAQAQQQACKNISSSNNKQLPSAGATSNNKQLHHMYSPLFVVNFLLMVVGSACGPLLLRAYFLHGGNRKWLSSLLQTAGWPLLLPALGFSFVSRRRRRKATKGATAAPLFLMSPRLLAATVAVGFMTGLDDLLYAYGLAYLPVSTSSILISTQLVFTAAFALLLVRQRFTAFSVNAVVLLSVGAAMLGMNAGGDRPAGVTKGQYVAGFAMTLGAAALYGLVLPVMELSQARHAARYGRPVTYALVMEMQAVIGLTATAFSAVGMLVNNDFQAIPGEAREFGLGQVGYYLLLAGSATVYQFFFLGTIGAIFYGSALLAGVIMTVLIPVTEVLAVMFFHEPFNGTKGIALALSLWGFVSYLYGEVRAKAHKSESDKPPNTVHTEHLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1IRR4</accession>
    <name>I1IRR4_BRADI</name>
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        <recommendedName>
            <fullName evidence="4">Phospholipase A(1) LCAT3</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">BRADI_4g35000v3</name>
    </gene>
    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003578373.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IRR4"/>
    <dbReference type="STRING" id="15368.I1IRR4"/>
    <dbReference type="EnsemblPlants" id="KQJ90987">
        <property type="protein sequence ID" value="KQJ90987"/>
        <property type="gene ID" value="BRADI_4g35000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826284"/>
    <dbReference type="Gramene" id="KQJ90987">
        <property type="protein sequence ID" value="KQJ90987"/>
        <property type="gene ID" value="BRADI_4g35000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826284"/>
    <dbReference type="eggNOG" id="KOG2369">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="OMA" id="MIDMLVK"/>
    <dbReference type="OrthoDB" id="190846at2759"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ90987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="52336" checksum="2D5D56BA4ECB9EAD" modified="2012-06-13" version="1">MAPSTMFGAAFGMRLRVLRRHFLRRRRRWRSRRRGGPARKGEEEEDEGREPVLLVSGMGGSVLHARRRSNPKFDLRVWVRIVLADLEFKKYLWSLYNVDTGCVEPLDDDVEIVVPEDDHGLFAIDILDPSWFVELLNLSMVYHFHDMIDMLVDCGYEKGTTLFGYGYDFRQSNRIDKAMAGLRAKLETAYKASGGKRVNIISHSMGGLLVRCFLSMNHDIFSKYVNKWICIACPFQGAPGCINDSLLTGLQFVYGFESFFFVSRWAMHQLLVECPSIYEMLPNPYFKWKEKPIIQVWRKNPEKDGLVELVQYEAADCVSLFEEALKNNELKYNGKTIALPFNMSIFKWATETRRILENAELPDTVSFYSIHGTSYGTPYDVCYGSESSPIGDLSEVCHTVPTYTYVDGDCTVPVESAMADGFAAKERVGVKADHRGLLCDENVFKLLKKWLGASEKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IRU4</accession>
    <name>I1IRU4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100832086</name>
        <name evidence="9" type="ORF">BRADI_4g35170v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family. Tig subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238370.1">
        <property type="nucleotide sequence ID" value="XM_010240068.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IRU4"/>
    <dbReference type="FunCoup" id="I1IRU4">
        <property type="interactions" value="1413"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IRU4"/>
    <dbReference type="EnsemblPlants" id="KQJ91027">
        <property type="protein sequence ID" value="KQJ91027"/>
        <property type="gene ID" value="BRADI_4g35170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832086"/>
    <dbReference type="Gramene" id="KQJ91027">
        <property type="protein sequence ID" value="KQJ91027"/>
        <property type="gene ID" value="BRADI_4g35170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832086"/>
    <dbReference type="eggNOG" id="ENOG502RYN3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093639_0_1_1"/>
    <dbReference type="OMA" id="EFCFDAM"/>
    <dbReference type="OrthoDB" id="1881930at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IRU4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1050:FF:000004">
        <property type="entry name" value="Trigger factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1050">
        <property type="entry name" value="Trigger factor ribosome-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005215">
        <property type="entry name" value="Trig_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008881">
        <property type="entry name" value="Trigger_fac_ribosome-bd_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036611">
        <property type="entry name" value="Trigger_fac_ribosome-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30560:SF5">
        <property type="entry name" value="OS09G0515400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30560">
        <property type="entry name" value="TRIGGER FACTOR CHAPERONE AND PEPTIDYL-PROLYL CIS/TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05697">
        <property type="entry name" value="Trigger_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102735">
        <property type="entry name" value="Trigger factor ribosome-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="Trigger factor ribosome-binding bacterial" evidence="8">
        <location>
            <begin position="79"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ91027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="198" mass="22351" checksum="5B3A4F5EE1E2ADA1" modified="2012-06-13" version="1">MFRKMMNHKCAKGLRPQLQYSHDLPSSNCLVMFNKQCSKRNNHEVCIVVQAISPVQCTENPMQAFISFEDFHVSVLTEEDGVIKTQIRVTVSSTMTDFIFQKVFAKHVAAAQPLPGFRQMKGGKTPDVPKEVALHLIGPSKVKKAAIKKIINRAVAEYVEKENLDVLKNLKVLQSYEELEAAFEPGKEFCFDATVHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I1ISS7</accession>
    <name>I1ISS7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g37850v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ISS7"/>
    <dbReference type="EnsemblPlants" id="KQJ91458">
        <property type="protein sequence ID" value="KQJ91458"/>
        <property type="gene ID" value="BRADI_4g37850v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ91458">
        <property type="protein sequence ID" value="KQJ91458"/>
        <property type="gene ID" value="BRADI_4g37850v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1181638_0_0_1"/>
    <dbReference type="InParanoid" id="I1ISS7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ91458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="235" mass="24259" checksum="04F8D7F92D0DC382" modified="2012-06-13" version="1">MAPELSIPVISRSATAMTEEEMTWLLSKFKEIEVQLAPLKDMAAILGVPWPSSAADTTTRVGTAAVADDTAPPALVPTTCSTSGPDTDAYPVHVPEASLLQGDVSLAVPAPAEASTTLSSPLPIFPEQDSSTPARCSMPGPDPCIPAVAASQLQKGKLLTSPAPGKASPTHVLLVCAVPKLVQASRPTCSMECPNDAANVLTSRSMLGTSLTTTTQVNNVHVKPDITTIDVILSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IX96</accession>
    <name>I1IX96_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein TIFY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Jasmonate ZIM domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100821983</name>
        <name evidence="9" type="ORF">BRADI_5g08650v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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        <subcellularLocation>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Vogel J.P."/>
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                <person name="Mockler T.C."/>
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        <source>
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                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </dbReference>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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</entry>
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    <organism evidence="9">
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">cv. Bd21</strain>
        </source>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <dbReference type="STRING" id="15368.I1IY38"/>
    <dbReference type="EnsemblPlants" id="KQJ82806">
        <property type="protein sequence ID" value="KQJ82806"/>
        <property type="gene ID" value="BRADI_5g11070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829165"/>
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        <property type="gene ID" value="BRADI_5g11070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829165"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307:SF4">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
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    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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            <end position="158"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ82806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="418" mass="45345" checksum="83381CF294C038C9" modified="2012-06-13" version="1">MDDDDVHGDVSPSPTPSPTSSSPLPVASALPVADPVTVASAPPGGGLMTLALPIQKHGVYPSHGGGGGGGGGREDAWSEGATSALIDAWGERFVALGRGSLRHPQWQEVADSVSARESYSKAAKSDIQCKNRIDTLKKKYKIERAKPVSSWQFYDRLDVLLAPTYNQKPSAHPNGRNPVPTALRAGFPKRSRTPLMPAAAPVKQRAPSPEPSASSESSDGFPPEPVLPVANGKRRRTDEGRGDGSSGNDRVQGLRELAQAIRRFGEAYERMETAKLEQATEMERRRLDFARELESQRVQFFLNTQRELSQAKKHNSLAAAASLAGATIVGGSSRRIASIPDPTSRRKTSVPTVRTSSNHHGRYHISESSRHQHHQHRPAPRPHYQYHDNNFAASAAANDGEQSDDEEEEDEGDEEESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IYU1</accession>
    <name>I1IYU1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribonuclease H2 subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ribonuclease HI subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100841903</name>
        <name evidence="9" type="ORF">BRADI_5g13357v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579966.1">
        <property type="nucleotide sequence ID" value="XM_003579918.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IYU1"/>
    <dbReference type="STRING" id="15368.I1IYU1"/>
    <dbReference type="EnsemblPlants" id="KQJ83146">
        <property type="protein sequence ID" value="KQJ83146"/>
        <property type="gene ID" value="BRADI_5g13357v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83147">
        <property type="protein sequence ID" value="KQJ83147"/>
        <property type="gene ID" value="BRADI_5g13357v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61298">
        <property type="protein sequence ID" value="PNT61298"/>
        <property type="gene ID" value="BRADI_5g13357v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841903"/>
    <dbReference type="Gramene" id="KQJ83146">
        <property type="protein sequence ID" value="KQJ83146"/>
        <property type="gene ID" value="BRADI_5g13357v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83147">
        <property type="protein sequence ID" value="KQJ83147"/>
        <property type="gene ID" value="BRADI_5g13357v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61298">
        <property type="protein sequence ID" value="PNT61298"/>
        <property type="gene ID" value="BRADI_5g13357v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841903"/>
    <dbReference type="eggNOG" id="KOG4705">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086483_0_0_1"/>
    <dbReference type="OMA" id="ANENNMA"/>
    <dbReference type="OrthoDB" id="29098at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09270">
        <property type="entry name" value="RNase_H2-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.530:FF:000002">
        <property type="entry name" value="Ribonuclease H2 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040456">
        <property type="entry name" value="RNase_H2_suB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019024">
        <property type="entry name" value="RNase_H2_suB_wHTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041195">
        <property type="entry name" value="Rnh202_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383:SF11">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09468">
        <property type="entry name" value="RNase_H2-Ydr279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17745">
        <property type="entry name" value="Ydr279_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rnh202 triple barrel" evidence="8">
        <location>
            <begin position="20"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease H2 subunit B wHTH" evidence="7">
        <location>
            <begin position="85"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="222"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ83146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="281" mass="31882" checksum="00B9382CC3AEED84" modified="2012-06-13" version="1">MASWCENMVLPPRVLIAPRPSSANGQGNILSLRHPKLEEEIQYLFSNGRLHEFNWFKERYGSWFLGDYVCEDGSIYYCTVVDPIFILLPLFEAARMSNGKDLGKFRQLDEILYIEGYPGYQHLMSIAGNHMELVCEVKEVANMKFFRLDDSKVLTWLCCKVHNIKEVIPKFGKNYAAQGERELLKDAIQIIRENLKDEPWLPVLCKKLQLDISEINETTKSNDTSFCAESSPIPAPARPSEGNIGNGSAKSSKGRPAKKLKAEVGSKNIKDMFRRVTRSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1J1G2</accession>
    <name>I1J1G2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="8">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100842827</name>
        <name evidence="10" type="ORF">BRADI_5g20734v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Extracellular space</location>
            <location evidence="8">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="8"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581616.1">
        <property type="nucleotide sequence ID" value="XM_003581568.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J1G2"/>
    <dbReference type="STRING" id="15368.I1J1G2"/>
    <dbReference type="EnsemblPlants" id="KQJ84412">
        <property type="protein sequence ID" value="KQJ84412"/>
        <property type="gene ID" value="BRADI_5g20734v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842827"/>
    <dbReference type="Gramene" id="KQJ84412">
        <property type="protein sequence ID" value="KQJ84412"/>
        <property type="gene ID" value="BRADI_5g20734v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842827"/>
    <dbReference type="eggNOG" id="ENOG502QQ71">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048041_0_0_1"/>
    <dbReference type="OMA" id="WNPKHIL"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02176">
        <property type="entry name" value="GH16_XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008263">
        <property type="entry name" value="GH16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01034">
        <property type="entry name" value="GH16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0052">Apoplast</keyword>
    <keyword evidence="8" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="1 8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982869" description="Xyloglucan endotransglucosylase/hydrolase" evidence="8">
        <location>
            <begin position="34"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="9">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="7">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ84412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="295" mass="33657" checksum="CAB8569B56FD431A" modified="2012-06-13" version="1">MAPLPCTEKLRCLWAPLLLWLAFLLLAVELGTADIYKDIELVWGASRTYFFMDGDSESLALSLDKSTGSCFKSKAMYLYAQIDLDIKLIEGDSAGTVCTVYTISEGPWEIHDEIDLEFLGNATGEPYTLHTNVFANGVGGREQQFQLWFDPSADYHTYSIIWNPKHILIQVDGVTIRDFKNNEAHGVAFPKWQQMRVYGSLWNADDWATQGGRVKTDWSKSPFVSYYRNYNVTWCQPSAGVDWCGNEPAGSRHFDLDQKAMSDLAWVRQRYMTYNYCSDYDRFNASTLPKECSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I1J292</accession>
    <name>I1J292_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100844733</name>
        <name evidence="22" type="ORF">BRADI_5g23027v3</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751316.1">
        <property type="nucleotide sequence ID" value="XM_014895830.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J292"/>
    <dbReference type="EnsemblPlants" id="KQJ84813">
        <property type="protein sequence ID" value="KQJ84813"/>
        <property type="gene ID" value="BRADI_5g23027v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844733"/>
    <dbReference type="Gramene" id="KQJ84813">
        <property type="protein sequence ID" value="KQJ84813"/>
        <property type="gene ID" value="BRADI_5g23027v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSMT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_116_4_1"/>
    <dbReference type="OMA" id="MSSANEM"/>
    <dbReference type="OrthoDB" id="660360at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000014">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000402">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1045">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095760" description="Receptor-like serine/threonine-protein kinase" evidence="18">
        <location>
            <begin position="22"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="20">
        <location>
            <begin position="22"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="21">
        <location>
            <begin position="335"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="494"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KQJ84813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="810" mass="90241" checksum="BD33B336869ABDF9" modified="2012-06-13" version="1">MAKHYIILIFFLLLISSFCKSDDQLTRTKPLTDHDILISKDGDFALGFFSPDSSNKSFYLGIWYHSIPGARTVVWVANRDDPITTPSSAKLAITNGSQMILSSSEGRNIWATTSNIATGGAEAYAVLLNTGNFVLRLPNTTDIWQSFDHPTDTILPTMKFWMNYKAQVIMRLVAWKGPDDPSSGDFSCSGDPSSPGLQWLIWHGTMAYARGTTLNGVSVTSSPYLSNASSVLYVTGVNLGDEFYFMLTVSNGLPLARVTLDYTGVLGFTSWNNHSSSWSVISENPKAPCDLYASCGPFSYCDLTGTAPKCQCLDGFEPNDFNFSRGCRRTLELKCDKQSRFVTLPRMKVPDKFLHIKNRSFDECTAECTGNCSCIAYAYANAGAATDSSRCLVWTGDLVDTGKTVNYGDNLYLRLTVDKKSSAIKIVLPIVACLLLLTCIALVCFCKYRGKRRKKEIEKKMMLEYFSTSNELEGEKTDFPFISFQDILWATNRFADSNLLGQGGFGKVYKGTLEGGNEVAVKRLSKGSGQGTLEFRNEVVLIAKLQHKNLVRLLGCCIHEDEKLLIYEYLPNKSLDAFLFDVARKYELDWSTRFKVIKGIARGLLYLHQDSRLTIIHRDLKASNILLDKEMIPKISDFGMARIFDANQNQANTIRVVGTYGYMSPEYVIGGAFSTKSDTYSFGVLLLEIVSGLKISSPQLIPNFSSLITYAWRLWDDKKATELVDSSVVDSCKIHEVLRCIHVGLLCVQDRPDDRPLMSSVMFALENESAVLPAPKQPVYFSPFNYKVGEARENMENSANPMSITTLEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EG67</accession>
    <name>A0A0Q3EG67_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_4g07290v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EG67"/>
    <dbReference type="FunCoup" id="A0A0Q3EG67">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ86710">
        <property type="protein sequence ID" value="KQJ86710"/>
        <property type="gene ID" value="BRADI_4g07290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ86710">
        <property type="protein sequence ID" value="KQJ86710"/>
        <property type="gene ID" value="BRADI_4g07290v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EG67"/>
    <dbReference type="OrthoDB" id="40134at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EG67">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="506"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="540"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="154"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ86710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="577" mass="62546" checksum="8D94392EA06C02FC" modified="2016-01-20" version="1">MHPGSLSCSAPRHEKDGAFSPTHAPPPCLSPTPASTTFSTLKLSSLHSPQHNATQRNALRELTPDPSPRAAMAAAEGAGEPELVSIPATPRGLSTPEGQSTPPVRRGAAAALASGAGTPVRRVVEGLRGYLEEVGHLTRLNPQDAWLPITESRSGNARYAAFHSLNAGLGFQALLLPLAFPCLGWSWGIISLTVAYFWQLYTLWILVKLHEAVPGRRYNRYVELAQAAFGEKLGVWLALFPTIYLSAGTATALILVGGETMKLFYQIVCGPLCSPSPISTVEWYLVFTSLSIILSQLPNLNSIAGISLIGGATAIMYCTMSWVLSVSQQRPPAISYEPVKYTSFGSSLFATLNALGIIAFAFRGHNLALEIQATMPSTFKHPAHVSMWRGAKVAYLLIAMCIFPVAIGGYWAYGNMVPPGGMLTAIYVFHSHDIPRALLAATFLLVVFNCLSSFQIYSMPVFDSFEAFYTGRTNRPCSVWVRSGFRVFYGFISLFIGVALPFLSSLAGLLGGLTLPVTFAYPCFMWICIKKPERFSFSWYLNWGLALLGTAFSVASSVGGVWSIINTGMKLKFFKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2024-05-29" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EL70</accession>
    <name>A0A0Q3EL70_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g17170v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EL70"/>
    <dbReference type="EnsemblPlants" id="KQJ88342">
        <property type="protein sequence ID" value="KQJ88342"/>
        <property type="gene ID" value="BRADI_4g17170v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88342">
        <property type="protein sequence ID" value="KQJ88342"/>
        <property type="gene ID" value="BRADI_4g17170v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EL70"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ88342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="82" mass="9591" checksum="AE30D037AB9F0CE5" modified="2016-01-20" version="1">MFSTIYPSKLMQPPISTLFFSAIADLVHHRQLHHQPFFQGKIHPTLLLMTYLVLVHQPQLLPNRQKTSQKQRDNLILITTMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ESI3</accession>
    <name>A0A0Q3ESI3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100823505</name>
        <name evidence="14" type="ORF">BRADI_4g31727v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Potassium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238226.1">
        <property type="nucleotide sequence ID" value="XM_010239924.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ESI3"/>
    <dbReference type="FunCoup" id="A0A0Q3ESI3">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3ESI3"/>
    <dbReference type="EnsemblPlants" id="KQJ90463">
        <property type="protein sequence ID" value="KQJ90463"/>
        <property type="gene ID" value="BRADI_4g31727v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823505"/>
    <dbReference type="Gramene" id="KQJ90463">
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F6H4</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
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                <person name="Vogel J.P."/>
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    </evidence>
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</entry>
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                <person name="Lail K."/>
                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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            <end position="121"/>
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        </location>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ97958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
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    <organism evidence="6">
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        <name type="synonym">Trachynia distachya</name>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GGJ3"/>
    <dbReference type="STRING" id="15368.A0A0Q3GGJ3"/>
    <dbReference type="EnsemblPlants" id="KQK10191">
        <property type="protein sequence ID" value="KQK10191"/>
        <property type="gene ID" value="BRADI_2g52566v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10191">
        <property type="protein sequence ID" value="KQK10191"/>
        <property type="gene ID" value="BRADI_2g52566v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GGJ3"/>
    <dbReference type="OrthoDB" id="941679at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GGJ3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000010">
        <property type="entry name" value="glucan endo-1,3-beta-glucosidase, basic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK10191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="40539" checksum="8CB84E65CA384F53" modified="2016-01-20" version="1">MATGYIKHGETPRLRTARPRLERTEERKTSSDILLLLQSIGKMTMIALWAAACGLVLFCSSRSGVEGIGVNYGLIGDNLPSPDKVVSLYIAHNITDVRLFHPDTAVLDALRGSGLGVVLGTLNSDLAPLASDASYAASWVASYVAPYASSVRFRYINAGNEVVPGEGSEHILEAIKNLESALSAAGLGNGDGPVRVTTAVATSVLGASFPPSHGAFSDAAMPVMAPLVSYLAERGSPLLVNAYPYFAYAADPASVPLDYALLSPSAAPAVTDDDGVQYVNMFDAIVDAVHAAVEKVTGSGQGVELVVSETGWPSGGGGYGASVENAAAYMNNLVRHVGSGTPRRPGKAVETYIFAMFNENQKPEGVERYFGLFQPDMTEVYHVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GRR5</accession>
    <name>A0A0Q3GRR5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g08135v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GRR5"/>
    <dbReference type="EnsemblPlants" id="KQK13109">
        <property type="protein sequence ID" value="KQK13109"/>
        <property type="gene ID" value="BRADI_1g08135v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13110">
        <property type="protein sequence ID" value="KQK13110"/>
        <property type="gene ID" value="BRADI_1g08135v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13109">
        <property type="protein sequence ID" value="KQK13109"/>
        <property type="gene ID" value="BRADI_1g08135v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13110">
        <property type="protein sequence ID" value="KQK13110"/>
        <property type="gene ID" value="BRADI_1g08135v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GRR5"/>
    <dbReference type="OrthoDB" id="2012261at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK13109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="53" mass="6011" checksum="98EC7AD13D888D28" modified="2016-01-20" version="1">MIVLGGYLYCLYFVAYVCNQSLVMLLDLHYTAQAKVYSVKKHGLIGGNERHNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H0Y6</accession>
    <name>A0A0Q3H0Y6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g02322v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81691.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H0Y6"/>
    <dbReference type="EnsemblPlants" id="KQJ81691">
        <property type="protein sequence ID" value="KQJ81691"/>
        <property type="gene ID" value="BRADI_5g02322v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81691">
        <property type="protein sequence ID" value="KQJ81691"/>
        <property type="gene ID" value="BRADI_5g02322v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H0Y6"/>
    <dbReference type="OrthoDB" id="677671at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF149">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="115"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ81691.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="377" mass="43330" checksum="C54273630010D79F" modified="2018-03-28" version="2">MAPPPWSDLPPELLGLVIDRLLSPSSVDDRGHWLRLLQLHILLPLSSSARHAHFRELCRSGHSPSLDRARFRAVCRSWKSAVTEHASAVPPRQTPWIVLADGSFLTLNGVQPLYRLPSFPQNARCIGSTDGWLALDSVDANNNAHAYSLHNPFSAATVPLRELDAVIGKVSRLFQVRKVLMRSTPDDVVALVTNNWNYPVILVRPGKGVWLPKPRTAQYVYIIDVAFLGDKLYGVTQHRPHPPMDYKFYVWSSDDEDDDEDENEDGHNDDDEFEDYYIVSQMDDKCAVHELMISTRDDMIHEPLHYWHVEAGYWWDRRSDTRRFSKSIPAHKEEESDVIYFIDTGDVYNMRTHTMSPSLRNIDHLYSTWIFSLEIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H1V3</accession>
    <name>A0A0Q3H1V3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_1g29424v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus outer membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM53 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
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                <person name="Shu S."/>
                <person name="Lindquist E."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
        </source>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="KQK06803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="106" mass="12621" checksum="6C51DEEB96C55CDF" modified="2016-01-20" version="1">MILSYWPPPLLVYILPKAASPQSLQYIHTGRLHRSILYACWPLMQTVEEYTYLVEVDMIGQIEDHDVNKFMDQINDFRSREKRREREEKRECSDISRYAEAVVGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IQ58</accession>
    <name>A0A0Q3IQ58_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">BRADI_2g01106v3</name>
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    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
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            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <strain evidence="2 3">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQK02380">
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    </dbReference>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK02380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="110" mass="13129" checksum="D7F6727414C11713" modified="2016-01-20" version="1">METGSRRFPRRRSPGSSSGRRRPKLRRRRRHRRQRLHRTARHCRLLRASIDYTRVCMKYEALLSMMTELDKDVVCLCMRQPHLATCYRPVSIRVSFSFRVSTEPRGPRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J5T9</accession>
    <name>A0A0Q3J5T9_BRADI</name>
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        <recommendedName>
            <fullName evidence="6">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="ORF">BRADI_3g05500v3</name>
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    <organism evidence="7">
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        <lineage>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
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        <property type="protein sequence ID" value="KQJ93576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003570297.4"/>
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    <dbReference type="FunCoup" id="A0A0Q3J5T9">
        <property type="interactions" value="1838"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93576">
        <property type="protein sequence ID" value="KQJ93576"/>
        <property type="gene ID" value="BRADI_3g05500v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93577">
        <property type="protein sequence ID" value="KQJ93577"/>
        <property type="gene ID" value="BRADI_3g05500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842553"/>
    <dbReference type="Gramene" id="KQJ93576">
        <property type="protein sequence ID" value="KQJ93576"/>
        <property type="gene ID" value="BRADI_3g05500v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g05500v3"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3J5T9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ93576"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK14635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J9R5"/>
    <dbReference type="EnsemblPlants" id="KQK14635">
        <property type="protein sequence ID" value="KQK14635"/>
        <property type="gene ID" value="BRADI_1g17714v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14635">
        <property type="protein sequence ID" value="KQK14635"/>
        <property type="gene ID" value="BRADI_1g17714v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="619048at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165:SF89">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="36"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="318" mass="34714" checksum="58185218A7AB8D83" modified="2016-01-20" version="1">MRAPRRHPWQAHSVIPWLPTFSTAPNQPPPILGNHASGCGAPGGIMVLINDCTAAIRLLNPITGQTADLPSVAPLMSRARTGRDSMSVVKLPGPDLSETTLWCYTSGVTTASWPPGSTARGHWEMVSRGTVLSSAFSINGKVYFADTDGLMFVDLDKPALRRLLRRRSLADTETVVHMADDGAGRLLVIINRLYFKKEATGDDRDYTVEFNVFRLDAEARKLVPVRNLGKRALFLGNYCGALVVSASASAPGTIVHNGIYFQHDGHPTFFVRRLSRRNNNSLAIKAPKGSFLDDLTSYVKWNGRKTIVAPAASIYGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JFZ2</accession>
    <name>A0A0Q3JFZ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Knottin scorpion toxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g59775v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JFZ2"/>
    <dbReference type="EnsemblPlants" id="KQK11372">
        <property type="protein sequence ID" value="KQK11372"/>
        <property type="gene ID" value="BRADI_2g59775v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11372">
        <property type="protein sequence ID" value="KQK11372"/>
        <property type="gene ID" value="BRADI_2g59775v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JFZ2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033725128" description="Knottin scorpion toxin-like domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="209"/>
        </location>
    </feature>
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        <location>
            <begin position="104"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK11372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="22610" checksum="1122116B0D5CEA29" modified="2016-01-20" version="1">MAFNGAHAFFTLGFLLLMASGEQSSAAAALGTCEFPSTTFRGRCQDGLCAAACHGEHFKGDASKPVIGGRCSDDYYRWLSGGDGGYHVGPRCICIVDCGGKDGKAPPPPPAEQYDPESGRHHHHHSPPPPPAQEPPAEKDEVESRYGEKWWHHHHHSPPPPPAQEPPAAKEEAESRYGEMWWHHHHKSPPPPPAEEPPAPEAPAIAVTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JH80</accession>
    <name>A0A0Q3JH80_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g32791v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK17163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JH80"/>
    <dbReference type="EnsemblPlants" id="KQK17163">
        <property type="protein sequence ID" value="KQK17163"/>
        <property type="gene ID" value="BRADI_1g32791v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17163">
        <property type="protein sequence ID" value="KQK17163"/>
        <property type="gene ID" value="BRADI_1g32791v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JH80"/>
    <dbReference type="OrthoDB" id="692410at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK17163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17642" checksum="D7833C3DEEA4E742" modified="2016-01-20" version="1">MAHYPRKDLGDFANYKGRIKVRTVLKKQQILATDECPFGCTASESPTHLALECSCSRLVFQMLGIDVVGVRQIDDLFIHEKTLIAQNKVAACEVVTVAVLWNIWLARNRKVFDDQIISVPMVVRQCSETLKLWCHRLKSKEKLAAQKWLEDWPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JUC0</accession>
    <name>A0A0Q3JUC0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c-553</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytochrome c553</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Soluble cytochrome f</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100829962</name>
        <name evidence="14" type="ORF">BRADI_1g24320v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions as an electron carrier between membrane-bound cytochrome b6-f and photosystem I in oxygenic photosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c family. PetJ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312452.1">
        <property type="nucleotide sequence ID" value="XM_024456684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JUC0"/>
    <dbReference type="STRING" id="15368.A0A0Q3JUC0"/>
    <dbReference type="EnsemblPlants" id="KQK15676">
        <property type="protein sequence ID" value="KQK15676"/>
        <property type="gene ID" value="BRADI_1g24320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829962"/>
    <dbReference type="Gramene" id="KQK15676">
        <property type="protein sequence ID" value="KQK15676"/>
        <property type="gene ID" value="BRADI_1g24320v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1930491at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JUC0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000021">
        <property type="entry name" value="Cytochrome c6, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023655">
        <property type="entry name" value="Cyt_C6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34688">
        <property type="entry name" value="CYTOCHROME C6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34688:SF2">
        <property type="entry name" value="CYTOCHROME C6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 12" id="KW-0349">Heme</keyword>
    <keyword evidence="7 12" id="KW-0408">Iron</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="13">
        <location>
            <begin position="74"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK15676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="178" mass="18805" checksum="F003C575FF51F85B" modified="2016-01-20" version="1">MHRLPLATRPPPAHRTSSLLSVPQRRRLVCCCSLNSLADAARRCAAAPLLAASLLLGAAAASPGIPAATPPAIAQPGGGAALFRKACIGCHDTGGNILQPGATLFMKDLERNGVATEEEIYNITYYGKGRMPGYGEKCTSRGQCTFGPRLPEDDIKMLAAFVKSQAENGWPKIEADME</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K915</accession>
    <name>A0A0Q3K915_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100821673</name>
        <name evidence="11" type="ORF">BRADI_1g57680v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751879.1">
        <property type="nucleotide sequence ID" value="XM_014896393.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K915"/>
    <dbReference type="STRING" id="15368.A0A0Q3K915"/>
    <dbReference type="EnsemblPlants" id="KQK20940">
        <property type="protein sequence ID" value="KQK20940"/>
        <property type="gene ID" value="BRADI_1g57680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821673"/>
    <dbReference type="Gramene" id="KQK20940">
        <property type="protein sequence ID" value="KQK20940"/>
        <property type="gene ID" value="BRADI_1g57680v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1937997at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K915">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000008">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000034">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="260"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK20940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="487" mass="54140" checksum="0278E323346B155F" modified="2016-01-20" version="1">MDSCNNPAGNLAAAMKTCCFCMHVAVCKIISAVTTLVIGPKWKLLLGWCTNGSNSNQLRSGLVAVVIGSGRVEFTATRLQSFLRSTAMAVIVDDAAYRTTAFGQYRLLLAICLDYDFFARTAREKVKNAKLEFEKTEDQLKVFQSCGQKIGEVVRQLDDERFIVKLGRGPRHEIVGCHSKVNRKNLTPNTRVCLDPTTHTIMRTLPREVDPIVYNMLREDPGNVSYSAVGGLSDQIRELRECIELPLMNPDLFLRVGIKPPKGVLLYGPPGTGKTLLARAIASNIDATFMKVVSSAIVRPYIGESSRLIREMFAYARDNQPCIIFMDEIDAIGGRRFSVGSSADREIQRTLMELLNQLDGFDELGKVKIIMATNRPDVLDPALLRPGRLDRKIEIPLPNEHSRLEILKIHAAEMAKHGEIDYEAAAKLAEGFNAADMRNVCTEAGMSAIRAERDYVMNEDFMNAVRKLSDVKKLESSAQYNANFGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KB71</accession>
    <name>A0A0Q3KB71_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g62191v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KB71"/>
    <dbReference type="FunCoup" id="A0A0Q3KB71">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK21652">
        <property type="protein sequence ID" value="KQK21652"/>
        <property type="gene ID" value="BRADI_1g62191v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21652">
        <property type="protein sequence ID" value="KQK21652"/>
        <property type="gene ID" value="BRADI_1g62191v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3KB71"/>
    <dbReference type="OrthoDB" id="683654at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK21652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="194" mass="20836" checksum="0DC7E69B4F7E7813" modified="2016-01-20" version="1">MATANEHVLVSNPDKLVLFAEISGMDSCDREPTVLLRLVVQLCSGYGAGLEDVDTMEDLICRVPLRGLGCQGTAERAFGELVASINNPMLRPEVTAAAKAAAARVRARCGDSAAVDELSSTGFLLRVMFIDAWDESSDGEFGEFARSGMFIGMWEDDDEVQDDDDDGSGVHFSVRQYNGGFARDGGGSSDFCNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KYG8</accession>
    <name>A0A0Q3KYG8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g27405v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A0Q3KYG8">
        <property type="interactions" value="791"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3KYG8"/>
    <dbReference type="EnsemblPlants" id="KQK16208">
        <property type="protein sequence ID" value="KQK16208"/>
        <property type="gene ID" value="BRADI_1g27405v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16209">
        <property type="protein sequence ID" value="KQK16209"/>
        <property type="gene ID" value="BRADI_1g27405v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16208">
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        <property type="gene ID" value="BRADI_1g27405v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16209">
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        <property type="gene ID" value="BRADI_1g27405v3"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KYG8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="PDRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030482">
        <property type="entry name" value="PDRG1"/>
    </dbReference>
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        <property type="entry name" value="P53 AND DNA DAMAGE-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21162:SF0">
        <property type="entry name" value="P53 AND DNA DAMAGE-REGULATED PROTEIN 1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="42"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK16209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="296" mass="32614" checksum="75F298C33798314B" modified="2016-01-20" version="1">MPTTARRSFISIDLENVLQYNNRGCILLHCLSLAARFPLRKTSRGRAPRRPNPRRSSSSTSERRHPLPPRPAAERPSPHAPSLVFLLVRAPPPQVKSVGSLASAASAIISGSVLSASAIASGGSALSKFIMDPNMKQLQEALVDIETDAEQLLLARHQLVENDRMRNANREALTALRKRARTSKTSVPSPFEIIMKEMEGSSGKHLVKEICPTCGNHDPKENTWLMFPGSDIFARVPFHVAHTVLDKDQERLDYDTKKLQSFVKEKSFVISEKGALADKISPGIVKSLVSLTDKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LSQ3</accession>
    <name>A0A0Q3LSQ3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Terpene cyclase/mutase family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g17507v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LSQ3"/>
    <dbReference type="STRING" id="15368.A0A0Q3LSQ3"/>
    <dbReference type="EnsemblPlants" id="KQJ95492">
        <property type="protein sequence ID" value="KQJ95492"/>
        <property type="gene ID" value="BRADI_3g17507v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95492">
        <property type="protein sequence ID" value="KQJ95492"/>
        <property type="gene ID" value="BRADI_3g17507v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LSQ3"/>
    <dbReference type="OrthoDB" id="21502at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LSQ3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016866">
        <property type="term" value="F:intramolecular transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF4220"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007658">
        <property type="entry name" value="DUF594"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="TERPENE CYCLASE/MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764:SF38">
        <property type="entry name" value="TERPENE CYCLASE_MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13968">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="476"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene cyclase N-terminal" evidence="3">
        <location>
            <begin position="15"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4220" evidence="4">
        <location>
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        </location>
    </feature>
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            <begin position="817"/>
            <end position="866"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ95492.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ95492"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="878" mass="100426" checksum="D4514DFC54EC8DE4" modified="2016-01-20" version="1">MHTLFCKHMTDIGQVIGVYDWSGNNPVIPELWILPQFLPIHPGKFWCFCRMVYMPMAYIYGQKIVGPISPTILAIRDELYKEEYSHIDWNKAHSSCAKEELTYPTSRGHNIAFAFLNKLVEPMLSCWPFTKIGERALISLMEHIHYEDENSNYVGLCPINKVLNMICCWIENPNSNSFKQHIPRIHDYLWISEDGMKTKVYSGCQSWETALIVQALCSLNLIEEVAPTLRKAHEFIKYSQVNQNFPSYKNYYRERSKGSWTLSNGENGWSIADTTAEAIKALLMLSDISPDLVGDPIKEERLHDAVDCLLSYVVTVAIYVFCKSWPSSADKKLLTAAILLFIPGILQCFAKPWALQNASFNSLASSDPVHKDANMNREEELENFVEKARIFIMDKDSASVEDKENLSIPKILFVDFTYTYSHRLTNLKSFCVLDDKEAYSSLKEGLSNIFDLLYTRKKTGPVEETDLKCREERGTLLWMISVLVPIPAIILFHISHKKAYNTSDIIITFMLMYSVVVLEYLDGGLIMILYSEWPNTVAQHSIIGHFAHNRRHNKLMSLARRLRCKDLIDEYWSMKPCHSSQNITMLVRQHVQNGWKLYITDAESYREFNDIKGQWTLEHEGCHPSLGWSLEKSFDESILLWHIATDFCFHSMGSSTSLVHGQCREISNYMVHLLFDNPEMLMAGSRKKLFKIACDELEDILKGEEAPVDEDKIAKKVIEKVQSTQGSKGSFSFVQDAWVLAEGLMNLGSAKMWKVIQGVWLEMMCFSAGRCRGYLHAKSLGYGGEYLSYVWLALAHSGMETFPERLQRTKRLHFGKEKTKHLPNRSKYNAAPSTSGMELPNEEERVADQSASRGTEEIIMAAPSASQDDRTVEIVVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3N248</accession>
    <name>A0A0Q3N248_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g55673v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3N248"/>
    <dbReference type="EnsemblPlants" id="KQK10710">
        <property type="protein sequence ID" value="KQK10710"/>
        <property type="gene ID" value="BRADI_2g55673v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10710">
        <property type="protein sequence ID" value="KQK10710"/>
        <property type="gene ID" value="BRADI_2g55673v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3N248"/>
    <dbReference type="OrthoDB" id="670445at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK10710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="104" mass="10460" checksum="098F90ACCA10BBB1" modified="2016-01-20" version="1">MADDLGDPAAQAAALAQQQQAAQLQAQQQAAQLQAQATAAAQAQTQALAAAQEVVKAAAAAGVNIDATGLVTDLNKQTQEKSTAPKGSFQSGNGKEGKEKKETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3N4A1</accession>
    <name>A0A0Q3N4A1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cytokinin dehydrogenase</fullName>
            <ecNumber evidence="4">1.5.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100826954</name>
        <name evidence="11" type="ORF">BRADI_2g60456v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-dimethylallyladenine + A + H2O = 3-methyl-2-butenal + adenine + AH2</text>
            <dbReference type="Rhea" id="RHEA:13625"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15825"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="EC" id="1.5.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.99.12" evidence="4"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565050.1">
        <property type="nucleotide sequence ID" value="XM_003565002.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3N4A1"/>
    <dbReference type="STRING" id="15368.A0A0Q3N4A1"/>
    <dbReference type="EnsemblPlants" id="KQK11478">
        <property type="protein sequence ID" value="KQK11478"/>
        <property type="gene ID" value="BRADI_2g60456v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826954"/>
    <dbReference type="Gramene" id="KQK11478">
        <property type="protein sequence ID" value="KQK11478"/>
        <property type="gene ID" value="BRADI_2g60456v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826954"/>
    <dbReference type="OrthoDB" id="415825at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3N4A1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019139">
        <property type="term" value="F:cytokinin dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009690">
        <property type="term" value="P:cytokinin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.462.10:FF:000001">
        <property type="entry name" value="Cytokinin dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.10">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016170">
        <property type="entry name" value="Cytok_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015345">
        <property type="entry name" value="Cytokinin_DH_FAD/cytokin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050432">
        <property type="entry name" value="FAD-linked_Oxidoreductases_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006093">
        <property type="entry name" value="Oxy_OxRdtase_FAD_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878:SF53">
        <property type="entry name" value="CYTOKININ DEHYDROGENASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878">
        <property type="entry name" value="GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09265">
        <property type="entry name" value="Cytokin-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00862">
        <property type="entry name" value="OX2_COVAL_FAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044546077" description="cytokinin dehydrogenase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="10">
        <location>
            <begin position="59"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK11478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="523" mass="57832" checksum="6CFF62E9CA6B4630" modified="2016-01-20" version="1">MVKASVVCCFKLLLLLALGGVTMHVPDADVLAPLESLQLDGRLTFHDVSAVARDFGNRCSLLPAAVLHPGSVSDVATAVRHVFQLGERSPLTVAARGHAHSLMGQSQAAGGIVVKMESLGGGMRVVSSRGVSPYVDAPGGELWINVLHETLKHGLAPKSWTDYLHLTVGGTLSNAGVSGQAFRQGPQVSNVNQLEIVTGRGDVVTCSPEENSDLFYGALGGLGQFGIITRARIALEPAPRMVRWIRVLYSDFTSFTEDQEMMISAEKTFDYIEGFVIINRTGILNNWRTSFKPQDPVQASQFQSDGRVLYCLEMTKNFNHDEADSMEQEVGVLLSRLRYIQSTLFHTDVTYLEFLDRVHSSELKLRAQGLWEVPHPWLNLLIPRSTIHKFAKEVFGKILKDSNNGPILLYPVNKSKWDNRTSVVIPDEEIFYLVGFLSSAPSLSGHGSVEHAVNLNNQIVDFCEKAGVGMKQYLAPYTTQQQWKAHFGARWETFERRKHMYDPLAILAPGQRIFPKASLPMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PUG9</accession>
    <name>A0A0Q3PUG9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_3g02705v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PUG9"/>
    <dbReference type="EnsemblPlants" id="KQJ93089">
        <property type="protein sequence ID" value="KQJ93089"/>
        <property type="gene ID" value="BRADI_3g02705v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93089">
        <property type="protein sequence ID" value="KQJ93089"/>
        <property type="gene ID" value="BRADI_3g02705v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3PUG9"/>
    <dbReference type="OrthoDB" id="1937564at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF115">
        <property type="entry name" value="OS02G0129200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="25"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="2">
        <location>
            <begin position="89"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ93089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="372" mass="40939" checksum="3DC73682EAC3B213" modified="2016-01-20" version="1">MENLTARPSKRMRRSQPAAWAAGGWSDLASELCGVILSRVPSLADRARFRCVCRQWRHAAKQQWWLLPPPFPWLFPTASDDDDRRRRRFVSLPDGATHHIAVPQINLDQYPTCEAFGEWLHFPNAETGTHLLVNPLVPSGSGATTIELPPMKLLKLVVCPGGDLVAAIACPSGSWSWSGVLRAPPRAAGLLLDIALHGNELYALYGRTTLYAYGLDDCEPRCVIADPRPITEAEERFIALHHYDIRYFLVPGGGGGGKKLLLVRSEGESFVVFEADGGTGRWSEVAGLDDGEALFVSANCSGALPVYGGAGNCVFFVWESPRPRYHWSVDRSGRSFRVYDMSTKTIRDAIVSPLKSHGCHTTGSWLFPSLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3Q2Q5</accession>
    <name>A0A0Q3Q2Q5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g19722v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3Q2Q5"/>
    <dbReference type="EnsemblPlants" id="KQJ95905">
        <property type="protein sequence ID" value="KQJ95905"/>
        <property type="gene ID" value="BRADI_3g19722v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g19722v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3Q2Q5"/>
    <dbReference type="Proteomes" id="UP000008810">
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="155"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ95905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="219" mass="22595" checksum="E18260E76DDC6351" modified="2016-01-20" version="1">MNSEMSATLPDLRGRGGEMGFGLSVEEGGGRGAAIAGGWRGRVAVGNRERRGNQGHAVTGGPAVEHVVTGGRAAADAARGHCVVDVRAASARRRLTTRGPPPSLRSQARAAAVVRLPHQRRLVRAAPRHNTHRPRHRAGAARGVQGGCCVSRRSDSRREATTTLAGDCRAGQGRAGGGSRGGAGHNEGFPTAVVGDFQTAEPSTWTVVRLIATLATTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3Q4D5</accession>
    <name>A0A0Q3Q4D5_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g23369v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ96497"/>
        <property type="gene ID" value="BRADI_3g23369v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ96497"/>
        <property type="gene ID" value="BRADI_3g23369v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ96497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="145" mass="14585" checksum="F617FE789E6F693B" modified="2016-01-20" version="1">MLLRDRRAADGRPPYPRPAADADSASRAGCHRLPRTTPAPAQPSASSPRAGCCPPPSARSSALAGQAPAASLRAAVPSGLPTPPPPGSASPAPPPPPVRVPVPPRIEATAAAADLGRPANPASPATPSPVLPPIDCNFGFQIWIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3Q5E0</accession>
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        <recommendedName>
            <fullName evidence="6">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003573921.3"/>
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    <dbReference type="FunCoup" id="A0A0Q3Q5E0">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3Q5E0"/>
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        <property type="protein sequence ID" value="KQJ96857"/>
        <property type="gene ID" value="BRADI_3g27470v3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0009664">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00837">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="30"/>
            <end position="259"/>
        </location>
    </feature>
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        <location>
            <begin position="49"/>
            <end position="164"/>
        </location>
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    <feature type="domain" description="Expansin-like CBD" evidence="8">
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            <begin position="174"/>
            <end position="253"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU365023"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ96857.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ96857"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="259" mass="27252" checksum="8A52A4F0C3E9AC8B" modified="2016-01-20" version="1">MTMEASKSLPAFLALAVAAALMFLAPASAGWSQGSATFYGGSDASGTMGGACGYGNLYFTGYGKSTAALSVAMFNDGASCGQCYQISCDYQTNPQWCRQGTSVTITATNLCPANYALPSNNGGWCNPPRAHFDMAEPAWLQIGVYQGGYIPVLYQRVSCVKQGGVRFTVTGKNYFELVLVSNVGGSGSVQAMWAKSANTGQVAMSRNWGANWQCLAGLVGQAITFGVTTTGGQTIVFQNVVPASWSFGMSFISNLQFTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QSN9</accession>
    <name>A0A0Q3QSN9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g13856v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3QSN9"/>
    <dbReference type="EnsemblPlants" id="KQK04514">
        <property type="protein sequence ID" value="KQK04514"/>
        <property type="gene ID" value="BRADI_2g13856v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04514">
        <property type="protein sequence ID" value="KQK04514"/>
        <property type="gene ID" value="BRADI_2g13856v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3QSN9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK04514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="160" mass="18210" checksum="5CA502EE44ED7629" modified="2016-01-20" version="1">MQMLKPCAWCIGAFMIPNTSKKCVAVLGKTSVQAHVNASFFVSFCLNTLVYSFVRKRHMEKAREGEETQGRNEGFQALRGRGRRLPIGSAWWFIRWGWHSTSEEEVSPTQEAPLRHPFLGDQAIAKSFLLPIHIHPHVCLNSFTFFWLSVVKGVDVFSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R4M9</accession>
    <name>A0A0Q3R4M9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100843345</name>
        <name evidence="8" type="ORF">BRADI_2g42023v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566817.1">
        <property type="nucleotide sequence ID" value="XM_003566769.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R4M9"/>
    <dbReference type="STRING" id="15368.A0A0Q3R4M9"/>
    <dbReference type="EnsemblPlants" id="KQK08466">
        <property type="protein sequence ID" value="KQK08466"/>
        <property type="gene ID" value="BRADI_2g42023v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843345"/>
    <dbReference type="Gramene" id="KQK08466">
        <property type="protein sequence ID" value="KQK08466"/>
        <property type="gene ID" value="BRADI_2g42023v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843345"/>
    <dbReference type="OrthoDB" id="693960at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="WRKY transcription factor 57"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
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    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF335">
        <property type="entry name" value="OS01G0584900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WRKY"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
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            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK08466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="191" mass="21287" checksum="5EFCD8D6EF34344C" modified="2016-01-20" version="1">MSSYLLSMSPVDEIGGYVDDGSDDMTAGYLSFDIDEYYLPEADHALYTGQQAAADPPVAHSLQADDADSYKSGSKAASSEGLSYHDKINNHQSLTSAGKSCKIAFKTRSEVEVMDDGYRWRKYGKKMVKNSPNPRNYYRCSSEGCRVKKRVERERDDARFVITTYHGVHDHPAPLPPRGCAGYSLAQPHYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S0C2</accession>
    <name>A0A0Q3S0C2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100828651</name>
        <name evidence="12" type="ORF">BRADI_1g41980v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010227687.1">
        <property type="nucleotide sequence ID" value="XM_010229385.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3S0C2"/>
    <dbReference type="FunCoup" id="A0A0Q3S0C2">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3S0C2"/>
    <dbReference type="EnsemblPlants" id="KQK18370">
        <property type="protein sequence ID" value="KQK18370"/>
        <property type="gene ID" value="BRADI_1g41980v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828651"/>
    <dbReference type="Gramene" id="KQK18370">
        <property type="protein sequence ID" value="KQK18370"/>
        <property type="gene ID" value="BRADI_1g41980v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="416741at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3S0C2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000004">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
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        <property type="entry name" value="RNase_III_sf"/>
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    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNASE II-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="11">
        <location>
            <begin position="38"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK18370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="324" mass="36511" checksum="AE86CE2CC37991AC" modified="2016-01-20" version="1">MDTPSPPAADNEMPREEESLEREEAPPWFAPPMGPEDVAAVETLLGYEFGDKSLVGEALTHGSYYYPYPPGDSTYERLEYLGDGVLTCLMSREVFLTYRSLPPGPLTRLRAANVDKEKLARVAVEHGIHRFLRHKAPQLDGQIDDFIKELCKYKYHSNGLLDAPKVLSDIVESLIGAIYLDSNFNQEVVWRVFRNLADPLISLETLGKHPVSELLEFCQKTRRGLQFVKDGWDKHLKVDVLVDGCGRQEVFLRSIGEGGGEVLRIAAVLTFFLGGSQQRHLDFSEVVFSFVAFVIFKSYSYDSLSANASILFQLVLLLRRLVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S9V4</accession>
    <name>A0A0Q3S9V4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g62646v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK21722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3S9V4"/>
    <dbReference type="EnsemblPlants" id="KQK21722">
        <property type="protein sequence ID" value="KQK21722"/>
        <property type="gene ID" value="BRADI_1g62646v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21722">
        <property type="protein sequence ID" value="KQK21722"/>
        <property type="gene ID" value="BRADI_1g62646v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="683390at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3S9V4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072:SF6">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="263"/>
            <end position="339"/>
        </location>
    </feature>
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            <begin position="66"/>
            <end position="124"/>
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        <location>
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            <end position="208"/>
        </location>
    </feature>
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        <location>
            <begin position="432"/>
            <end position="458"/>
        </location>
    </feature>
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        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
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            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK21722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="563" mass="62173" checksum="1C1ABC6F3B09A04F" modified="2016-01-20" version="1">MTVETGEHSHALAVVVLDHELAALALLPHGEGDVGLLMVLGLLQPPEPPFARPSCRSGALCAAARRADGSGSGSTDRGGRGTTAAGGHESTDGGGWGTTASGGCTSRRKGNAGVVGAPRRRSEDEALRRIKDESLCVPFHDKDCIGFDIVQKFLAGTEAGRRACFSRPKWTACSILQRQLPERGNKSPRLRLSKPSSPRHSPVLGRPRGGTGYCGSLPHPLPACFVANSEMAEQSLSKGRGHRTQPRGLVDTEVGGVTEGSHHEIGFRTQKAWSKEAWRNIVCGLNQKFSVSFTISQVKQKEQDLKKDYRSVKSLLDEGSFGWDSERNMVDAPDTVWASFASRRNIKDALQWRDKSFPYYDELIPLYEGRYAEGRTSHGKEQYASMSNHASTLSSHPAEVTEAYHVPSPTIPVLNESDLHFTLDDEVEDANLDPHPLSFTPRQHTQAPPRPAQTSSGKCCTWPRKRQKCDCDNGFLERYLKMKQEEIDLFAAIDKKLEGPYSITECITAIQKLDGLQIGDMLMAADIFRCRDNREIFMSFSRDELRLAWLTREIARNQTNDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHU5</accession>
    <name>A0A2K2CHU5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g17635v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHU5"/>
    <dbReference type="EnsemblPlants" id="PNT61605">
        <property type="protein sequence ID" value="PNT61605"/>
        <property type="gene ID" value="BRADI_5g17635v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61605">
        <property type="protein sequence ID" value="PNT61605"/>
        <property type="gene ID" value="BRADI_5g17635v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CHU5"/>
    <dbReference type="OrthoDB" id="682412at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT61605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="120" mass="14071" checksum="A97E7568B2BCDC80" modified="2018-03-28" version="1">MPDTKHLSCYESNPMDAKHIWCEVALWPNCSDITTAICHVSASIDSFYERMISATQSKHRQGIKSTFILVSWAIWRERNSRVFNDKEILFRQICCFIKDEAREWAFADAKALRKLLWEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CLB2</accession>
    <name>A0A2K2CLB2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Auxin transport protein BIG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100830348</name>
        <name evidence="18" type="ORF">BRADI_4g08567v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the UBR4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A2K2CLB2">
        <property type="interactions" value="1836"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CLB2"/>
    <dbReference type="EnsemblPlants" id="PNT62813">
        <property type="protein sequence ID" value="PNT62813"/>
        <property type="gene ID" value="BRADI_4g08567v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62813">
        <property type="protein sequence ID" value="PNT62813"/>
        <property type="gene ID" value="BRADI_4g08567v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="30336at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
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    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="1424"/>
            <end position="1495"/>
        </location>
    </feature>
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        <location>
            <begin position="2462"/>
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            <begin position="4424"/>
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            <begin position="2999"/>
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            <end position="4759"/>
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        <source>
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        <source>
            <dbReference type="EMBL" id="PNT62813.1"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="4950" mass="552135" checksum="CC299D85C1518823" modified="2018-03-28" version="1">[encoded data, 4950 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CM43</accession>
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        <submittedName>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT63098"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CML2</accession>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">BRADI_4g13583v3</name>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="BRADI_4g13583v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="93" mass="10236" checksum="3EF321D305ABE827" modified="2018-03-28" version="1" fragment="single">RWERRATKFVKTAGSNRPSSASATPSTPRGASTRFQSPQAAMDPGMSFMELLASVAGSAPTACGRSGLYMTQIWNREIRRLKMKSISEPAFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMY0</accession>
    <name>A0A2K2CMY0_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g15005v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CMY0"/>
    <dbReference type="EnsemblPlants" id="PNT63382">
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        <property type="gene ID" value="BRADI_4g15005v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63382">
        <property type="protein sequence ID" value="PNT63382"/>
        <property type="gene ID" value="BRADI_4g15005v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CMY0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT63382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="78" mass="8725" checksum="3FE82EB5FA4ED533" modified="2018-03-28" version="1">MISAIPIEVLPKSSQANGALNVELIQIYKGNQDAPMPMASRIFLIKEKTFFFCLPHPLLMNLHLNLPENHTLTVVSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CN84</accession>
    <name>A0A2K2CN84_BRADI</name>
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        <recommendedName>
            <fullName evidence="6">CASP-like protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">LOC112268850</name>
        <name evidence="3" type="ORF">BRADI_4g16581v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310794.1">
        <property type="nucleotide sequence ID" value="XM_024455026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CN84"/>
    <dbReference type="EnsemblPlants" id="PNT63483">
        <property type="protein sequence ID" value="PNT63483"/>
        <property type="gene ID" value="BRADI_4g16581v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112268850"/>
    <dbReference type="Gramene" id="PNT63483">
        <property type="protein sequence ID" value="PNT63483"/>
        <property type="gene ID" value="BRADI_4g16581v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="685197at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573:SF63">
        <property type="entry name" value="CASP-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573">
        <property type="entry name" value="CASP-LIKE PROTEIN 4A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044576568" description="CASP-like protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="18793" checksum="47EE47E08E422A3C" modified="2018-03-28" version="1">MSANKASSSHGAVLLPMATLLLRGLVGLLCTASRRFVSVALAGLPEFVETCRPTFSSLEQHERTGEHVSWVAGVGCAYSLLAMAFVTFNVVQRKKMIGGSLRGTVFLISADVACSALVIARAAAALGFTVENEMRVEVFDLTNMGFYELVQISCLLLLMATVCMVVIIMLTVSLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQ71</accession>
    <name>A0A2K2CQ71_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g25441v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQ71"/>
    <dbReference type="EnsemblPlants" id="PNT64171">
        <property type="protein sequence ID" value="PNT64171"/>
        <property type="gene ID" value="BRADI_4g25441v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64171">
        <property type="protein sequence ID" value="PNT64171"/>
        <property type="gene ID" value="BRADI_4g25441v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQ71"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT64171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="180" mass="19282" checksum="5AD285EE63C3C6B0" modified="2018-03-28" version="1">MTWMHFPNTAVRRPKMPSLGCGESSPARLALPAIVRSASVFPASGEVFPANSTALLVLDHRFHYQPPHELILPLPLPPACLAGAGASCCRGRVQCNGKMRNAATSSVKRGQNRFKSATSAQKPLHGVIYQLNQYLGGGRPEVLGGGTYALLPASYRYTQLDSSSVTVPGQKFCQPEPPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CS72</accession>
    <name>A0A2K2CS72_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA topoisomerase</fullName>
            <ecNumber evidence="7">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100836701</name>
        <name evidence="11" type="ORF">BRADI_4g34290v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="7"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CS72"/>
    <dbReference type="STRING" id="15368.A0A2K2CS72"/>
    <dbReference type="EnsemblPlants" id="PNT64879">
        <property type="protein sequence ID" value="PNT64879"/>
        <property type="gene ID" value="BRADI_4g34290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64879">
        <property type="protein sequence ID" value="PNT64879"/>
        <property type="gene ID" value="BRADI_4g34290v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="430051at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CS72">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000002">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056452">
        <property type="entry name" value="Zn_ribbon_TOP3B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF20">
        <property type="entry name" value="DNA TOPOISOMERASE 3-BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23546">
        <property type="entry name" value="Zn_ribbon_TOP3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="9">
        <location>
            <begin position="139"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="10">
        <location>
            <begin position="300"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="966"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="966"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="991"/>
            <end position="1003"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT64879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1003" mass="112133" checksum="9783E8D588711F8D" modified="2018-03-28" version="1">MMSRPLMMGRMARCWMAEGFSYPYSKMPRRTSSRSPISSKLVTTSTPREVAIPRPRRDVEALGIVAAAIGPSRCGGDKSKRRDCYKGGSSRLVRTELFALANLPHQHFSSHRCRSRYRAVGNGGRSNPTSRNLLTLGSPSLQVAEKPSIALSIASAISGGRMSTRKGSTDVHEFDGTFQGSQAFFRVTSVIGHVFSVDFPPAYQNWEGTDPVDLFNAPVLRSECNPKVHIRRHLAQEARGCTYLILWLDCDREGENICYEVINCTGIPENEVGRRIFRAKFSSVTEKDISNAMDNLVLPNKDEALSVDARQEIDLKVGVAFTRFQTRYFQGKYGNLDSRVISYGPCQTPTLGFCVQRYQQINTFKPEKFWSLRTYVIKDGDEIQLEWDRKKLFDFDVTVMFQKMVANDGTLIVTDISVKEECKARPSGLNTVNLLKVASSALGIGPQTAMHMAERLYTQGYISYPRTESTAYPASFDFRSVLSALVHNPLWSNNIRTLMDAGFVKPRQGHDAGDHPPITPMRSASEEALETDAWRLYQYICQHFIGTVSPDCRYTRTAVEFTSGGEIFRCVGHRVTSKGFTSIMPWLAVAENNLPTFKNGDTVSIHKVDIYEGSTTPPDYLSESELISLMEKNGIGTDASIPVHINNISERNYVQVNSGRRLVPTALGTTLIRGYQCIDADLCLPDIRSFIEQQITLIAKGKADHLQVIQHVIQQFMKKYSYFVKKIENMDALFEAQFSPLADSGRLLSKCGKCGRYMKYISTQPMRMYCITCEEVYYLPQNGTIKLYKEIICPLDGFELLLFSMVGPDAKSFPLCPCCYNSPPFEGIDKLFGAIKLDDTGKLGKGAGMPCFLCPHPTCKQSMITQGVCACPECSGTLILDPVSAPKWRLLCNMCNCVVLLPHAAHRITTTDKKCSTCESTIIEVDFNKKATPLEDGATLHEGCILCDELLHSLIEMKHGKSFFMRRGRGRGRGRARGRGGSRGRGRRGNSRHDDPKMSFRDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CSE4</accession>
    <name>A0A2K2CSE4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g35200v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CSE4"/>
    <dbReference type="EnsemblPlants" id="PNT64933">
        <property type="protein sequence ID" value="PNT64933"/>
        <property type="gene ID" value="BRADI_4g35200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64933">
        <property type="protein sequence ID" value="PNT64933"/>
        <property type="gene ID" value="BRADI_4g35200v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CSE4"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CSE4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055312">
        <property type="entry name" value="FBL15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709:SF26">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709">
        <property type="entry name" value="OS10G0396666 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="21"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT64933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="442" mass="48930" checksum="AB7E27BC06C63952" modified="2018-03-28" version="1">MKKRNADGTLKLKPVESSREVDRLSDLPDDLIGRVLGFLPTPQAVLTSRLSRRWRRVWPAHVRALNLSVYDGVGRRLSGLSSGALARFATPGIPSISLHIASHVDIATGDTWYTEAMERASGSVSITAPRVLNWLKLPRWEALSLLLNDHGVELQLPPLGVVRFGRLAELALSTVRLPSGTPPLHEFLSACCPRLRRLRLCAVRGDAVRALDLRSDALESLDVNNVDQLVTLHVAAPNLRSLSVRSCFRFPVQSDVETEVVVSAPRMEAVCWYRSYPKRLDFLTADPTAHQVRRLSGLKLPTRGPSGRFDFPYTIQLLRAFSVTAEQLELDLILPDDMTLLNWAGQAAGGEDLLSYVPRLPRVSALSLNGLRWGLGCSVRPSLAELLSRVPNVTRLYVESNPYCRTVFESQGTVPPRGEYQWGCVDVGAEEKLRLNSLRALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CY09</accession>
    <name>A0A2K2CY09_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g18572v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CY09"/>
    <dbReference type="FunCoup" id="A0A2K2CY09">
        <property type="interactions" value="1060"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66918">
        <property type="protein sequence ID" value="PNT66918"/>
        <property type="gene ID" value="BRADI_3g18572v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66918">
        <property type="protein sequence ID" value="PNT66918"/>
        <property type="gene ID" value="BRADI_3g18572v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CY09"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF28">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="137"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="210"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="245"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="315"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="350"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="385"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="420"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="455"/>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="490"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="525"/>
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        </location>
    </feature>
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            <begin position="560"/>
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        </location>
    </feature>
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        <location>
            <begin position="595"/>
            <end position="629"/>
        </location>
    </feature>
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        <location>
            <begin position="630"/>
            <end position="664"/>
        </location>
    </feature>
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        <location>
            <begin position="665"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="700"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT66918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="796" mass="87725" checksum="9F40CB1B78AFB8C4" modified="2018-03-28" version="1" fragment="single">MATLRLRHFSSTPPSSPRRLRSALASATERVRAGTLTPDDAHHLFDGLLRQTAQVPESVLNGFLSALARAPASAALRDGPALAVALFNRMSTGTGPRVALPTVYTYSILIDCCCRARRLDLAFAFFGCLLRQGMKADVIVVSSLLRGLCDAKRTDEAVDVLFHRMPELGCVPDAISYSTVLKSVCDDGRSQWALDILRMAVKQGGGCPCNVVVYSTVVHGLFKEGKVGEACDLFHEMTQQGVPPNVVTYNSVIHALCKARAVDKAQGILRQMVGNGVQPDNVTYNTLIHGYSTLGQWKQAVRMFKEMTSRGVIPNTVTCSTFVAFLCKHGRIEEAREFFDSMLAKGHKLNIISYSTLLHGYATAGCLVDMSNLFNLMVRDGIVPNQHVFNILVNGYAKCGMVREAMFIFEDMQKRGLNPDVLTYLAVIHAFCRMGSMDDAMDKFNHMIDKGVEPNFAVYQCLIQGFCTHGDLVKAEELVYEIRNKGLGPCILSFASLINHLCKEGRVFEAQRIFDMIIRTGEKADVNIFTSLIDGYCLIGKMSEAFRVHDAMVSVGIEPDIVTYGTLVNGCCKNGRIDDGLILFRELLHKGVKPTTFTYGIILDGLFHAGRTAAAKEMFQEMIESGIAVTIPTYSILLTGLCRNNCTEEAITVFQKLCAMNVKFDIVILNIMISKMFKARRREEAEGLFASIPDYGLVPTVQTYTIMMENLIKEGSVEEAEGVFSVMLKSGLSPTSHFINVIVRTLLEKGEIVKAGIYMCRVDGKSILFEASTASMLLSLFSCKGKHREHLNLLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CY72</accession>
    <name>A0A2K2CY72_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g19182v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CY72"/>
    <dbReference type="EnsemblPlants" id="PNT66984">
        <property type="protein sequence ID" value="PNT66984"/>
        <property type="gene ID" value="BRADI_3g19182v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66984">
        <property type="protein sequence ID" value="PNT66984"/>
        <property type="gene ID" value="BRADI_3g19182v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CY72"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT66984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="142" mass="16534" checksum="C5EC24D3D23B5F8D" modified="2018-03-28" version="1">MGDCLEHTWTLQKIEQFHNFLLMQKCPGLLLPALRNNMLLTYTLKEILPKMCCFHVAIVSKKITSSSTFWCLQCREHPYAAIIPAQYMSAEVNYHAYIHISEEHACQKSQNRCLLTRVLERGDHLTVHWRDQRSVLNYCAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D0J9</accession>
    <name>A0A2K2D0J9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g32250v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT67795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D0J9"/>
    <dbReference type="EnsemblPlants" id="PNT67795">
        <property type="protein sequence ID" value="PNT67795"/>
        <property type="gene ID" value="BRADI_3g32250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67795">
        <property type="protein sequence ID" value="PNT67795"/>
        <property type="gene ID" value="BRADI_3g32250v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D0J9"/>
    <dbReference type="OrthoDB" id="671853at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT67795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="64" mass="7373" checksum="A695C42877E8EFE3" modified="2018-03-28" version="1">MQSRGASIPRFAFDFSMFDQLSSKDNQSKLLLDLIGVISYVGPYDYASPTSQYKLRKIHIRNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D2H4</accession>
    <name>A0A2K2D2H4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100832348</name>
        <name evidence="5" type="ORF">BRADI_3g40998v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68479.1"/>
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    <dbReference type="GeneID" id="100832348"/>
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        <property type="gene ID" value="BRADI_3g40998v3"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00443">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="202"/>
            <end position="956"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="353"/>
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        </location>
    </feature>
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        <location>
            <begin position="705"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="399"/>
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        </location>
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            <begin position="721"/>
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            <begin position="755"/>
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        </location>
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            <begin position="769"/>
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            <begin position="821"/>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DBL2</accession>
    <name>A0A2K2DBL2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g33323v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="70" mass="8079" checksum="FF53CB83CF31BE2C" modified="2018-03-28" version="1">MILLIRRQDVIDDKHTTVRVLSFVVRALDNGYGLVYPRLRGLSKRIKIFSSSNLTCLGVFQFYLIHSPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DBR6</accession>
    <name>A0A2K2DBR6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g34775v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DBR6"/>
    <dbReference type="STRING" id="15368.A0A2K2DBR6"/>
    <dbReference type="EnsemblPlants" id="PNT71736">
        <property type="protein sequence ID" value="PNT71736"/>
        <property type="gene ID" value="BRADI_2g34775v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71736">
        <property type="protein sequence ID" value="PNT71736"/>
        <property type="gene ID" value="BRADI_2g34775v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DBR6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038863">
        <property type="entry name" value="Put_Complex_I_su8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36401">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36401:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="103" mass="10888" checksum="1F2E22CCFE5155AF" modified="2018-03-28" version="1">MAGRLTAMGSRVLGGHGAAARAATSALRHRAGMGLPAGRHIVPDKPLPTNDELVWDNGTPFPEQGRRAQFCRPANPPAITAGAAAARVPIRWSLSRSRKTDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DDL0</accession>
    <name>A0A2K2DDL0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g42938v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0A2K2DDL0"/>
    <dbReference type="EnsemblPlants" id="PNT72349">
        <property type="protein sequence ID" value="PNT72349"/>
        <property type="gene ID" value="BRADI_2g42938v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72349">
        <property type="protein sequence ID" value="PNT72349"/>
        <property type="gene ID" value="BRADI_2g42938v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DDL0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT72349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="78" mass="8555" checksum="8203DE7755394AA6" modified="2018-03-28" version="1">MASDGSFCTGVIASVRCLASTVQRELCPNRMLHANAIDIPPKSESESPHMDLVLSTSIGPKPFFTTEYFIFFGFLVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DHD9</accession>
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        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g62098v3</name>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DHD9"/>
    <dbReference type="EnsemblPlants" id="PNT73674">
        <property type="protein sequence ID" value="PNT73674"/>
        <property type="gene ID" value="BRADI_2g62098v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73674">
        <property type="protein sequence ID" value="PNT73674"/>
        <property type="gene ID" value="BRADI_2g62098v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DHD9"/>
    <dbReference type="OrthoDB" id="603189at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35828:SF44">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35828">
        <property type="entry name" value="OS08G0203800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT73674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="285" mass="31721" checksum="C1C56C5E716D4818" modified="2018-03-28" version="1">MGEISWLPDDMLRDILLRFRGDAVALFRCATVCKRWRRLVADPSFLRRCWPHHDGRSRSSLAGFFALSRALFGRRGRRGLKSFIPAAAAGLFNHAVPLAWRHGHLLVRLSPHGGGDTAVIRLAVCDLLTGACHVFPPLNPAREFVVHNKDGYAILTGADCGQRRSPFFKVVVFNVAYMAPRMMARQDDESTDVQAAPERCLCTKTVELRHPEGGKKAGVVKKCGKLLVTDNQDNVYAADLGTGTMEEVTAWPGRRRFNRDDADEVVPLEMDWPATFVSRLGDRAP</sequence>
</entry>
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    <name>A0A2K2DK91_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100831408</name>
        <name evidence="4" type="ORF">BRADI_1g20410v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DK91"/>
    <dbReference type="FunCoup" id="A0A2K2DK91">
        <property type="interactions" value="2053"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DK91"/>
    <dbReference type="EnsemblPlants" id="PNT74693">
        <property type="protein sequence ID" value="PNT74693"/>
        <property type="gene ID" value="BRADI_1g20410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74693">
        <property type="protein sequence ID" value="PNT74693"/>
        <property type="gene ID" value="BRADI_1g20410v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="767702at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DK91">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="DnaJ_domain"/>
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        <source>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
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        <name type="synonym">Trachynia distachya</name>
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            <taxon>BOP clade</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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            <end position="64"/>
        </location>
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        <location>
            <begin position="194"/>
            <end position="355"/>
        </location>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23635"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT75201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="370" mass="41999" checksum="FCC6F70F7454F0A3" modified="2018-03-28" version="1">MAADKRPRPSSPSPPATAAVSKVLDDSDLLHEILLRLDFPTWLVRAALVCKRWLSHVSDPAFLRRFQERHPPRLLGFYLESACKAFQQFVPLPDLPPELAAVARTASFDLTAEFYMHTSIEHCRNGVLLLRHTGYDKFIYQVRSPLNLARDGAVVATCCKSMADEERHYGYMLLPNDGGHGMSVNFVGSHLELFAKLYMLGTTGYILGLELASTRLFFIELPDAIRYEPPGSLQLSLKMSHQTVNSGVYLLNLEGLKINVWLRSTDDNSSWLLVDTICLRQVFGHLVKPSWESGASRINLAGSGDNAEFVFLEVDAEIFCMYIRSRTVVKVYEMEYKDDQLFQIYPFMMVWPPIFPALIQRRDQVEISES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DPU7</accession>
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        </submittedName>
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    <organism evidence="1">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT76294">
        <property type="protein sequence ID" value="PNT76294"/>
        <property type="gene ID" value="BRADI_1g46835v3"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT76294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="69" mass="7784" checksum="0CBBEEFC944C8AEF" modified="2018-03-28" version="1">MVDRLSVDVFVVRQLPLSDGCWRRTSWTVEVTGGRRPPSMHEIQFRVQAHGGRRRLLPVQASKTAAARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DSV1</accession>
    <name>A0A2K2DSV1_BRADI</name>
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        <recommendedName>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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    <reference evidence="6 7" key="1">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_014896591.2"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A2K2DSV1"/>
    <dbReference type="EnsemblPlants" id="PNT77361">
        <property type="protein sequence ID" value="PNT77361"/>
        <property type="gene ID" value="BRADI_1g61653v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843529"/>
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        <property type="protein sequence ID" value="PNT77361"/>
        <property type="gene ID" value="BRADI_1g61653v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843529"/>
    <dbReference type="OrthoDB" id="44867at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46554">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 26A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 26B-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="Med26"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="175"/>
        </location>
    </feature>
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        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
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        <location>
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            <end position="259"/>
        </location>
    </feature>
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        <location>
            <begin position="318"/>
            <end position="354"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT77361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="366" mass="40768" checksum="7BEF1E8F62509E82" modified="2018-03-28" version="1">MAAPPRSLDYWRGFFNGAQASIFDAIDAAIRVAAADHPDGLRARRAAIAEHLYTVLPPSEEAVWPAPAFAGAPPLQEQHGSSDGPAVSTHRDSSGDPVVAEAVRVKAALSSNQQKSEDELLDLLRRLQLLQFTVDTVRVTEIIKAVQPLRKHASKQIRQLAGSLIEGWQATVNEWMNNEAAIIDHTPQSMDVSCLEQEEGGLPSPPMDEAALFATSFAYRELSEFFDEMDDDGNTIINGKEDEQQYPTNEDSVKEQPSMVQQYDPVQNWRLDQSAVRQSRLHELSGWQVSHQSMTEAQGKPSNAAFGLIRPSRLHSEPIGSKIKISPKQLQDISVAHSQRRPKPTMPNQRSNEEYKCWIRKTCRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DVM0</accession>
    <name>A0A2K2DVM0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g77415v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DVM0"/>
    <dbReference type="EnsemblPlants" id="PNT78324">
        <property type="protein sequence ID" value="PNT78324"/>
        <property type="gene ID" value="BRADI_1g77415v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78324">
        <property type="protein sequence ID" value="PNT78324"/>
        <property type="gene ID" value="BRADI_1g77415v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DVM0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036043462" evidence="1">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT78324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="56" mass="6235" checksum="9BDCA3AE1D56E17A" modified="2018-03-28" version="1">MLIFLILMLITDLSTFAPGALSTCQTTGSLEEKKYSLHFTKVTEIQSISQSQLRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1GL27</accession>
    <name>I1GL27_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100842692</name>
        <name evidence="2" type="ORF">BRADI_1g02300v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757045.1">
        <property type="nucleotide sequence ID" value="XM_014901559.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GL27"/>
    <dbReference type="FunCoup" id="I1GL27">
        <property type="interactions" value="664"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK12227">
        <property type="protein sequence ID" value="KQK12227"/>
        <property type="gene ID" value="BRADI_1g02300v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842692"/>
    <dbReference type="Gramene" id="KQK12227">
        <property type="protein sequence ID" value="KQK12227"/>
        <property type="gene ID" value="BRADI_1g02300v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842692"/>
    <dbReference type="eggNOG" id="ENOG502R3N4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137068_0_0_1"/>
    <dbReference type="OMA" id="CACASIE"/>
    <dbReference type="OrthoDB" id="685203at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007789">
        <property type="entry name" value="DUF688"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35466:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35466">
        <property type="entry name" value="SERINE/ARGININE REPETITIVE MATRIX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05097">
        <property type="entry name" value="DUF688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK12227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="151" mass="15492" checksum="B11350DB73BBEA23" modified="2012-06-13" version="1">MAPEESFRRPCSVPFKWELQPGVPKQQQPPAGSHGAAAAGAPPSSSASIALRLPPRLAPPPAAAAVSSCVPSVSGGGLAASASARWRSCAPAAATAPKTTAAAAGRHRRSMSARFATSLAALPFARRRQGRYSREDDDVAFLAIYGDDLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMH0</accession>
    <name>I1GMH0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100840554</name>
        <name evidence="9" type="ORF">BRADI_1g06320v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the intermembrane transfer of phosphatidylglycerol and phosphatidylinositol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558016.1">
        <property type="nucleotide sequence ID" value="XM_003557968.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMH0"/>
    <dbReference type="EnsemblPlants" id="KQK12840">
        <property type="protein sequence ID" value="KQK12840"/>
        <property type="gene ID" value="BRADI_1g06320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840554"/>
    <dbReference type="Gramene" id="KQK12840">
        <property type="protein sequence ID" value="KQK12840"/>
        <property type="gene ID" value="BRADI_1g06320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840554"/>
    <dbReference type="eggNOG" id="KOG4680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097982_1_0_1"/>
    <dbReference type="OMA" id="NHELSCI"/>
    <dbReference type="OrthoDB" id="6409159at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GMH0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00917">
        <property type="entry name" value="PG-PI_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.770:FF:000002">
        <property type="entry name" value="putative phosphatidylglycerol/phosphatidylinositol transfer protein DDB_G0282179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033917">
        <property type="entry name" value="ML_PG-PI_TP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF0">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL_PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093932" description="MD-2-related lipid-recognition domain-containing protein" evidence="7">
        <location>
            <begin position="27"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="8">
        <location>
            <begin position="29"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK12840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="154" mass="16591" checksum="EAAFDFF9CEB09E06" modified="2012-06-13" version="1">MASDRRLHDLLLLAAALLLSASSATAADFGYCKKGRHYPVQVSSVEIVPEPIVRGEPATFKISASTDKSITKGKLVIDVTYFFFHVHSETHDFCAGTTCPATGEFVLAQEQTLPSFTPPGSYTLEMKLLGDKNEVLTCISFGFSIGFISPVAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNV5</accession>
    <name>I1GNV5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BRADI_1g10320v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the two pore domain potassium channel (TC 1.A.1.7) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNV5"/>
    <dbReference type="FunCoup" id="I1GNV5">
        <property type="interactions" value="1668"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13465">
        <property type="protein sequence ID" value="KQK13465"/>
        <property type="gene ID" value="BRADI_1g10320v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13465">
        <property type="protein sequence ID" value="KQK13465"/>
        <property type="gene ID" value="BRADI_1g10320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821349"/>
    <dbReference type="eggNOG" id="KOG1418">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033675_0_1_1"/>
    <dbReference type="OMA" id="FCFFLVR"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF137">
        <property type="entry name" value="TWO PORE POTASSIUM CHANNEL B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="330"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK13465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="362" mass="39319" checksum="EEFCBC879D8A529D" modified="2012-06-13" version="1">MAANGVTTPLLHHCDDQASTEKPPGSATGRRFRLRHCRTAPSPDPAAPGEPPPPRPSNDIRSAPPKRLFESASRPSFRLVGVLLLSYLLAGSTAFYLAMDQMSGHRSASRALDALYFCVVTMTTVGYGDLVPVTDAAKLLAAAFAFAGVAVVGTFLSKAADYLVEKQESLLFRAVHAHENKRHPRLLRATEEANRTRYKLYVSGALLALLVAAGTLFLWKAEGMRALDAFYCACATVTTLGYGDRSFASAPGRAFAAAWVTASTVVVALFFLYAAELCAEGRQRELARWVATRRMTTTDLEAADLDGDRRVGKADFVLYKLKELGKIGQEEIEEFLEEFDRLDADHSGTLSPYDLAVAQPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPW5</accession>
    <name>I1GPW5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine dioxygenase</fullName>
            <ecNumber evidence="3">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_1g13400v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:20442"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPW5"/>
    <dbReference type="FunCoup" id="I1GPW5">
        <property type="interactions" value="264"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GPW5"/>
    <dbReference type="EnsemblPlants" id="KQK13924">
        <property type="protein sequence ID" value="KQK13924"/>
        <property type="gene ID" value="BRADI_1g13400v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13924">
        <property type="protein sequence ID" value="KQK13924"/>
        <property type="gene ID" value="BRADI_1g13400v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4281">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061320_4_1_1"/>
    <dbReference type="InParanoid" id="I1GPW5"/>
    <dbReference type="OMA" id="MNTAGHR"/>
    <dbReference type="OrthoDB" id="271433at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070483">
        <property type="term" value="P:detection of hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20289">
        <property type="entry name" value="cupin_ADO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012864">
        <property type="entry name" value="PCO/ADO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22966">
        <property type="entry name" value="2-AMINOETHANETHIOL DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22966:SF66">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07847">
        <property type="entry name" value="PCO_ADO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK13924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="297" mass="32801" checksum="B8E8B6CCD5BB2DA5" modified="2012-06-13" version="1">MVGKNGAPAPVPMQLIPLGRRAREAADREKAEAAAKERAERGTKRKREALAQKKTQTRSNLRRLVDALRVVFPEGSKCDTLPSAHSVSLICRLLEEIRPEDVGLRPDGARYFQELDERGARGTPAITTKNLYECDTFTVAAFLLSPGKVMPLHDHPRMTVFSKILMGSAHVTSYDWVQPSLCLDRRWLLAQKVLDKEFTPESGAWALYPDTGGNVHRLAAGADGPCAFIDVLSPPYGSPLQQIGGSFYEDMPFKKIHPAVPNQISEEQKGKLAWLQQIGVPKDLNLITLQNRGPGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQ48</accession>
    <name>I1GQ48_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100828163</name>
        <name evidence="4" type="ORF">BRADI_1g14110v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
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        <property type="protein sequence ID" value="KQK14061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_1g14110v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g14110v3"/>
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        <property type="term" value="P:leaf vascular tissue pattern formation"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0010087">
        <property type="term" value="P:phloem or xylem histogenesis"/>
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    </dbReference>
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        <property type="entry name" value="PH_pln"/>
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        <property type="entry name" value="VAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008546">
        <property type="entry name" value="VAN3-bd-like_auxin_canal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31351:SF4">
        <property type="entry name" value="AUXIN CANALIZATION PROTEIN (DUF828)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31351">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Auxin_canalis"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="19"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Pleckstrin-like plant" evidence="3">
        <location>
            <begin position="345"/>
            <end position="449"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="104"/>
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        </location>
    </feature>
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        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK14061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="48327" checksum="04A621F73CDA35E9" modified="2012-06-13" version="1">MDGHPRARLAGAGGDLRPPEPPLDPLEFLSRSWSASSVDVPRARPGAPPPLPALAGPIAEDAACELDDGGAATAGSSFSFASAATSQFIMERILAQSEVAPLTSGRLSHSSGPLTGGGSLTDSPPVSPEIDDSQYCRAAGTPKPQAYRGGSKTVGRWLKDRKEKKKEETRAHNAQVHAAVSVAAVAAAVAAVAAATAAASGSGKDDRAARTDMAMASAATLVAAQCVEAAESMGAEREHLEAVVSSAVNVRTPGDIVTVTAAAATALRGAATLRARALKEVWNIAAVIPVEKGTMGGGGHHHKQSVHKQHHKLESNDSSVSDLSLDEENNFLGICSQEFLVRGTELLKRTRKGALHWKVVSVYINRLGLVALKMKSRHVAGTITKKKKNVVIDVCKDVAAWPGRHLLEDGEHRRYFGLKTADHRVIEFECTSQRDYELWTKGVARLLSIAGERKRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRZ9</accession>
    <name>I1GRZ9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100829568</name>
        <name evidence="5" type="ORF">BRADI_1g20330v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003562571.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRZ9"/>
    <dbReference type="FunCoup" id="I1GRZ9">
        <property type="interactions" value="2080"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GRZ9"/>
    <dbReference type="EnsemblPlants" id="KQK15053">
        <property type="protein sequence ID" value="KQK15053"/>
        <property type="gene ID" value="BRADI_1g20330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829568"/>
    <dbReference type="Gramene" id="KQK15053">
        <property type="protein sequence ID" value="KQK15053"/>
        <property type="gene ID" value="BRADI_1g20330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829568"/>
    <dbReference type="eggNOG" id="ENOG502QV1M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025169_0_0_1"/>
    <dbReference type="OMA" id="WRFRSKK"/>
    <dbReference type="OrthoDB" id="2015179at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055502">
        <property type="entry name" value="DUF7074"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055503">
        <property type="entry name" value="DUF7075"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31469:SF4">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31469">
        <property type="entry name" value="OS07G0633600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23269">
        <property type="entry name" value="DUF7074"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7074" evidence="3">
        <location>
            <begin position="153"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7075" evidence="4">
        <location>
            <begin position="279"/>
            <end position="573"/>
        </location>
    </feature>
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            <begin position="1"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
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            <begin position="12"/>
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        </location>
    </feature>
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        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF23269"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK15053.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK15053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="590" mass="66700" checksum="7FA7E54A3FFCB718" modified="2012-06-13" version="1">MSSSDQEEEYHDEGSPLFRDDSEDSGRRRRSSSGPSSAAVGEVPVSQSIIKAASNVCFSLFVLAVLAITVAAVTYQPPDPWLQSSAALTTSLSRVLPNSTFLVPDDSLLPTGEDFNSSSSSAAAQRDATDQAAAADATPAAAAGSCDDNDPDAPVNCSDPRVLAAVKAFNAKAFFRKSIVFLSYEAPVPGPKPGECDVAWRFRNRREKSWRRYRDYRRFALTAGEGCALEIVSVGKFRSGKNAARSPRRTKGPKSPHVAAPPVDAEINDTVPVVGSEAEFRKGKYLYYMRGGDHCKSMNQFIWSFLCGLGEAKFLNRTFVMDLNMCLSGAHTEDGRDVDGKDFRFYFDFEHLKDSVSLVEEGDFLKDWRRWDKKKGPGRITVRKVPTYKVTPMQLRREKSNIIWRQFDGKEPENYWYRVCEGRAAKVIQRPWYAIWKSKRLMNIVTEIAGRMDWDYDGLHVVRGWKAQNKKMYPNLDDDTSPGAIVEKVTKLIKPMRNLYIATNEPFYNYFDKLRSYFHVHLLDDYKELWSNTSEWYNETTTITGGKPVPFDAYMKVIVDTEVFYRSKAKVETFNNLTRDCKDGINTCNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSJ0</accession>
    <name>I1GSJ0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MADS-box transcription factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100836119</name>
        <name evidence="10" type="ORF">BRADI_1g21980v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2014" name="PLoS ONE" volume="9" first="e84781" last="e84781">
            <title>Genome-wide analysis of the MADS-box gene family in Brachypodium distachyon.</title>
            <authorList>
                <person name="Wei B."/>
                <person name="Zhang R.Z."/>
                <person name="Guo J.J."/>
                <person name="Liu D.M."/>
                <person name="Li A.L."/>
                <person name="Fan R.C."/>
                <person name="Mao L."/>
                <person name="Zhang X.Q."/>
            </authorList>
            <dbReference type="PubMed" id="24454749"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KF469300">
        <property type="protein sequence ID" value="AIG21814.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK15334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NM_001302866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSJ0"/>
    <dbReference type="STRING" id="15368.I1GSJ0"/>
    <dbReference type="EnsemblPlants" id="KQK15334">
        <property type="protein sequence ID" value="KQK15334"/>
        <property type="gene ID" value="BRADI_1g21980v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836119"/>
    <dbReference type="Gramene" id="KQK15334">
        <property type="protein sequence ID" value="KQK15334"/>
        <property type="gene ID" value="BRADI_1g21980v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836119"/>
    <dbReference type="eggNOG" id="KOG0014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="WGDEYGI"/>
    <dbReference type="OrthoDB" id="1933443at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GSJ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000003">
        <property type="entry name" value="MADS-box transcription factor MADS-MC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="89"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AIG21814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK15334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="309" mass="35159" checksum="111D61AF19ECA6E1" modified="2012-06-13" version="1">MGRGPVQLRRIENKINRQVTFSKRRNGLLKKAHEISVLCDAEVALIVFSTKGKLYEYSSQDSSMDVILERYQRYSFEERAVLDPNIGDQANWGDEYGRLKIKLDALQKSQRQLLGEQLEPLTTRELQQLEQQLDSSLKHIRSRKNQLLFDSISELQKKEKSLKDQNGVLQKHLVETEKEKNNALSNIQHREQLNEKNTALPNTHDREQQNGATTSSPSPTPLTVLDSMPNLNIGYYASHIHCIDVCSRHINLEKQEENQNLSRLQHKQTAASYRHGCSAPSVTDEGFQCPHVTLHPKGISVPSGVHHGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSS9</accession>
    <name>I1GSS9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100825270</name>
        <name evidence="3" type="ORF">BRADI_1g22770v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562774.2">
        <property type="nucleotide sequence ID" value="XM_003562726.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSS9"/>
    <dbReference type="EnsemblPlants" id="KQK15438">
        <property type="protein sequence ID" value="KQK15438"/>
        <property type="gene ID" value="BRADI_1g22770v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825270"/>
    <dbReference type="Gramene" id="KQK15438">
        <property type="protein sequence ID" value="KQK15438"/>
        <property type="gene ID" value="BRADI_1g22770v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825270"/>
    <dbReference type="eggNOG" id="ENOG502R3M5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1066935_0_0_1"/>
    <dbReference type="OMA" id="VRPAIWS"/>
    <dbReference type="OrthoDB" id="695484at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37206">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37206:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK15438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="235" mass="25266" checksum="9F8360C5398BF0C2" modified="2012-06-13" version="1">MDERLPPPPQFQLVSPSSMSAPASPYSALHPLLLPSPNPHLLLKPKTLTLSLSSSSLASIPSSSSPAPAASDAWELVQPTVTVAAAAPIDGGLDDCAIFPPRLHEGLGLEGEEEEAAAKEEKDEEETDDEEVEWIWGWGRWRAAARRAWAAGAEGVLVHGECGCPGVRPAVWSAAAAAVVVGALLYVRRRDRRERDLLVLLSQEKDKRIAQLLHQIALLSDIRSGSEAIKILRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSX4</accession>
    <name>I1GSX4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100834774</name>
        <name evidence="8" type="ORF">BRADI_1g23200v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562804.2">
        <property type="nucleotide sequence ID" value="XM_003562756.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSX4"/>
    <dbReference type="EnsemblPlants" id="KQK15490">
        <property type="protein sequence ID" value="KQK15490"/>
        <property type="gene ID" value="BRADI_1g23200v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834774"/>
    <dbReference type="Gramene" id="KQK15490">
        <property type="protein sequence ID" value="KQK15490"/>
        <property type="gene ID" value="BRADI_1g23200v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834774"/>
    <dbReference type="OrthoDB" id="679615at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094072" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="6">
        <location>
            <begin position="16"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="165"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK15490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="224" mass="23583" checksum="6BC04A8687B8C00A" modified="2012-06-13" version="1">MALIFILFFLATAAADSELPPLWNFPPLPPLPPLPSLPPLPALPPLPPGTPPSNGPQPSPSPPPETSSCMLPLLRAAPCLDFLTNTSVPAPSSRCCDRFRSLVGNASESICICHLVLSDPNVTSIVGNVDLGRLVALQLRCNVLVPPMLIFSCVSRPIPPLTMIPSSEPAASMSTESSYEHFDLGTMSASHEKVQQPELVSTIEQPPPSPPPPKPAGTGENKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1GU27</accession>
    <name>I1GU27_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100827650</name>
        <name evidence="14" type="ORF">BRADI_1g26510v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563040.1">
        <property type="nucleotide sequence ID" value="XM_003562992.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GU27"/>
    <dbReference type="STRING" id="15368.I1GU27"/>
    <dbReference type="EnsemblPlants" id="KQK16058">
        <property type="protein sequence ID" value="KQK16058"/>
        <property type="gene ID" value="BRADI_1g26510v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827650"/>
    <dbReference type="Gramene" id="KQK16058">
        <property type="protein sequence ID" value="KQK16058"/>
        <property type="gene ID" value="BRADI_1g26510v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827650"/>
    <dbReference type="eggNOG" id="ENOG502QTII">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024953_3_3_1"/>
    <dbReference type="OMA" id="HFFLIAY"/>
    <dbReference type="OrthoDB" id="941679at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GU27">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000009">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000002">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094095" description="glucan endo-1,3-beta-D-glucosidase" evidence="12">
        <location>
            <begin position="29"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="13">
        <location>
            <begin position="390"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK16058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="538" mass="56743" checksum="A2BBFD4D5DA4EB81" modified="2012-06-13" version="1">MGSRSRGGRSSSGLLLLSLHLLLGLHSSLRCFVSAAGGGGSGEPYVGVTIGTAVTNLLSPSDLAAFLRAQRITRVRLYDADPRLLSALASSGARAIVGVPNDELLALGSSPATASAWVSRRVLPFAGANSSSPGLISAIAVGDEVPTALPSALPVLLPAIQSLAAALAAANLSSIPVSTPLPFSIVLDPFPPSQAYFNQSLAKSFILPLLSHLANTSAPLMLNLYPYYSLMQSSGVVPVDNAMFKPLPPSLEMVDPNTLLHYTNVFDAMLDAVYVALKNLNVSAPVPVLVTETGWPSYGDRKEEPYASRDNANTYNSNLIKHVVTDKPGTPMRPGAQASVYIYELFNEDLRPGPVSEANWGLFHGNGTPVYLLHASGADGFLGNATTDRTFCIASDDADEKAVQAAMDWACGPGRSDCTAIQPGEGCYQPNDVRSHASFAFDTYYQSQGKAGGSCYFQGAGMVTTTDPSHDSCIFPGSKLYSNATKSDGANTTSSQTSDAEGSAIWRLRTGRGEGFLLVFRFLLSVMVVIMTNSNLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1GY35</accession>
    <name>I1GY35_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100823549</name>
        <name evidence="6" type="ORF">BRADI_1g38170v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563773.1">
        <property type="nucleotide sequence ID" value="XM_003563725.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GY35"/>
    <dbReference type="FunCoup" id="I1GY35">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GY35"/>
    <dbReference type="EnsemblPlants" id="KQK18036">
        <property type="protein sequence ID" value="KQK18036"/>
        <property type="gene ID" value="BRADI_1g38170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823549"/>
    <dbReference type="Gramene" id="KQK18036">
        <property type="protein sequence ID" value="KQK18036"/>
        <property type="gene ID" value="BRADI_1g38170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823549"/>
    <dbReference type="eggNOG" id="KOG1843">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025299_2_0_1"/>
    <dbReference type="OMA" id="HICRAAQ"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11526">
        <property type="entry name" value="SYLF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000151">
        <property type="entry name" value="Zinc finger family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF35">
        <property type="entry name" value="OS06G0552400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="5">
        <location>
            <begin position="215"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK18036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="524" mass="56953" checksum="6CB6C7F5878C312D" modified="2012-06-13" version="1">MEGADHLYPSIEPYDLEPPQVGIAAAGETRVCDQTVRWDEEPKEGTHLFEQGTKDNPDTNNVEDCLGGVDIYECNDETITMKEYGEDISPDCTLREQIGIWIPPSVPPMIKHDHEEWQKGFGSNGGYFPEEEYQWDIDEEIREMTMWDVFAEMAVAGKDKLISIASYDFGRHSMSLISHFLLQAALEDEARTLAEASAGSEHALLEAEPTKWLPDSAASSCMLCGARFHPIICTRHHCRFCGGIFCGGCSKGRSLMPPKFGTSDPQRVCDVCGVRLECIQPRLMNKISRACQLPTKDLTDLSTLRSWINIPWAHTMEYEIYKAANSIHGYCKIGKLNPEKSIPDSILRQAKGLAIITVVKVGVMITYKIGTGLVIARRADGSWSPPSAISTCGIGYGAQAGGELADFIIVLRNTDAIKTFSGNAHLSVGAGIGASAAHLGRVAEADFRAGDGGYAACYTYSCSKGAFVGCALNGSLVSTRNSENARFYGGPIKASEILLGSLAKPPAAATLYKALSILFEKIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYB2</accession>
    <name>I1GYB2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100835921</name>
        <name evidence="4" type="ORF">BRADI_1g39150v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563809.1">
        <property type="nucleotide sequence ID" value="XM_003563761.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYB2"/>
    <dbReference type="STRING" id="15368.I1GYB2"/>
    <dbReference type="EnsemblPlants" id="KQK18152">
        <property type="protein sequence ID" value="KQK18152"/>
        <property type="gene ID" value="BRADI_1g39150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835921"/>
    <dbReference type="Gramene" id="KQK18152">
        <property type="protein sequence ID" value="KQK18152"/>
        <property type="gene ID" value="BRADI_1g39150v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835921"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042473_0_0_1"/>
    <dbReference type="OMA" id="NIKMQNG"/>
    <dbReference type="OrthoDB" id="7763451at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12459">
        <property type="entry name" value="RRM1_CID8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12460">
        <property type="entry name" value="RRM2_CID8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000530">
        <property type="entry name" value="Polyadenylate-binding protein-interacting protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034823">
        <property type="entry name" value="CID8-like_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034825">
        <property type="entry name" value="CID8-like_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343:SF22">
        <property type="entry name" value="LD29830P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343">
        <property type="entry name" value="SERINE/ARGININE-RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="166"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="263"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="119"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK18152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="351" mass="38267" checksum="B58308FB9029CA27" modified="2012-06-13" version="1">MVAVAADGLIRADAVPAAAGAGTGAERVDAAAAGGAGAAKAREDEVREYKSDVRKLEELFKKLNPSAEEFVPLSRRQADGGRRLSADAPVFVSPAIDYYAPHHPFQQQQPQQMHVLQVVGGGGRDSSSDGSVNGQPNRRRRNGFNQGRRRMGPRPRRTDREDSVRRTVYVSDIDQHVTEQKLAEVFSNCGQVVDCRICGDPNSVMRFAFIEFADDVGARAALSLAGTILGYYPVRVLPSKTAILPVNPKFLPRTEDEKEMVSRTVYCTNIDKNVPEDVVKKFFEGICGEVARLRLLGDYVHSTCIAFVEFVQAEGAIMALNCSGMLLGSLPVRVSPSKTPVRPRSPRAMSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYZ8</accession>
    <name>I1GYZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RIN4 pathogenic type III effector avirulence factor Avr cleavage site domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100826114</name>
        <name evidence="2" type="ORF">BRADI_1g43430v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563945.1">
        <property type="nucleotide sequence ID" value="XM_003563897.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYZ8"/>
    <dbReference type="STRING" id="15368.I1GYZ8"/>
    <dbReference type="EnsemblPlants" id="KQK18574">
        <property type="protein sequence ID" value="KQK18574"/>
        <property type="gene ID" value="BRADI_1g43430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826114"/>
    <dbReference type="Gramene" id="KQK18574">
        <property type="protein sequence ID" value="KQK18574"/>
        <property type="gene ID" value="BRADI_1g43430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826114"/>
    <dbReference type="eggNOG" id="ENOG502S4YA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169219_1_0_1"/>
    <dbReference type="OMA" id="NHKHTAT"/>
    <dbReference type="OrthoDB" id="1903947at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GYZ8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040387">
        <property type="entry name" value="RIN4/NOI4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008700">
        <property type="entry name" value="TypeIII_avirulence_cleave"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33159:SF100">
        <property type="entry name" value="OS06G0286375 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33159">
        <property type="entry name" value="RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05627">
        <property type="entry name" value="AvrRpt-cleavage"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIN4 pathogenic type III effector avirulence factor Avr cleavage site" evidence="1">
        <location>
            <begin position="6"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK18574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="80" mass="8862" checksum="10B819D6AC038527" modified="2012-06-13" version="1">MSEESGRPLPKFGEWDVNDPASADGFTVIFNKARDEKKAGNGQDTESPCKDTRTERVESYAAKTNSKKWFCCVTPSPTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1H095</accession>
    <name>I1H095_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC100845353</name>
        <name evidence="24" type="ORF">BRADI_1g47097v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560978.1">
        <property type="nucleotide sequence ID" value="XM_003560930.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H095"/>
    <dbReference type="EnsemblPlants" id="KQK19242">
        <property type="protein sequence ID" value="KQK19242"/>
        <property type="gene ID" value="BRADI_1g47097v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845353"/>
    <dbReference type="Gramene" id="KQK19242">
        <property type="protein sequence ID" value="KQK19242"/>
        <property type="gene ID" value="BRADI_1g47097v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845353"/>
    <dbReference type="eggNOG" id="ENOG502QPTD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_22_0_1"/>
    <dbReference type="OMA" id="IPETLFC"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000363">
        <property type="entry name" value="Leucine-rich repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000358">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001028">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF89">
        <property type="entry name" value="OS06G0585982 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="669"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="980"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="994"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="697"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQK19242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1007" mass="107534" checksum="14982AFD136850F1" modified="2012-06-13" version="1">MAKLPIIVIVVIFHVGLIVLLPIAVAAMAPVAGPVPDEDLSALLAFCSSVSSDPGGALADWGRSPAFCNWTGVACNSSSSTRRVTQLVLSGRGIRGVISPALGKMAFLTVLDLSSNGFAGEIPSELSALSRLTQLSLTNNLLSGAIPAGIGLLPELYYLDLSGNRLTGGIPETLFCNCSALQYMDLSNNSLAGDIPYADECRLPSLRFLLLWSNSLSGPIPRAISNSAALEWVDLESNYLAGELPHNVFDRLPRLQFLYLSYNNFSSSHGNTNLDPFFQSLSNCTRLQELELAGNGLGGPLPPSIGELSRGLRQLHLEDNAISGSIPPNISGLVNLTYLNLSNNHLNGSIPPEISRLRLLERLYLSNNFLSGEIPRSIGELPRLGLVDLSGNILAGAIPDTFSNLTQLRRLMLHHNRLTGAIPPSLGDCQNLEILDLSYNGLRGEIPAHVVAGLSSLKIYLNLSSNHLQGALPIELSKMDMVLALDLSSNEIAGGIPSQLGACVALEYLNLSRNALRGALPSSVAALPFLRAIDVSRNELSGALPEPALRASTSLRDADFSYNDFSGVVPVLPNLPGAEFRGNPGLCVIAACGGGSRRRHRRAVVPAVVSIVGAVCAMLCAAAGCRWVAAVRARRRESTWRVDVEGQGEREHHHPRISYRELSEATGGFEETSLIGAGRFGRVYEGTLRGGARVAVKVLDPKLGGGGGEVSVSFRRECEALRRTRHKNLIRVITTCSTPSFHALVLPLMPRGSLEDHLYPRDRERHGGPEGLDFRQLVSVASDVAEGMAYLHHYSPVRVVHCDLKPSNVLLDDGMRAVISDFGIARLVAGAGAGETTSSTTSDESAPCNNSIATGLLQGSVGYIAPEYGLGGNPSARGDVYSFGVMLLQLITGKRPTDVIFDEGLTLHDWVRRHHPHDIAAALAHAPWARRDAAAANGMVAVELIELGLACTHYSPALRPTMEDVCHEITLLREDLAKHGGAHDNEDDSGGGRSFSTTKDSLFSNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1H0T2</accession>
    <name>I1H0T2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Starch synthase, chloroplastic/amyloplastic</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100826095</name>
        <name evidence="15" type="ORF">BRADI_1g48610v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Plastid</location>
            <location evidence="11">Chloroplast</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Plastid</location>
            <location evidence="11">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561063.1">
        <property type="nucleotide sequence ID" value="XM_003561015.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H0T2"/>
    <dbReference type="STRING" id="15368.I1H0T2"/>
    <dbReference type="EnsemblPlants" id="KQK19495">
        <property type="protein sequence ID" value="KQK19495"/>
        <property type="gene ID" value="BRADI_1g48610v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826095"/>
    <dbReference type="Gramene" id="KQK19495">
        <property type="protein sequence ID" value="KQK19495"/>
        <property type="gene ID" value="BRADI_1g48610v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826095"/>
    <dbReference type="eggNOG" id="ENOG502QTWM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_18_2_1"/>
    <dbReference type="OMA" id="TWCPWYM"/>
    <dbReference type="OrthoDB" id="512920at2759"/>
    <dbReference type="UniPathway" id="UPA00152"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009501">
        <property type="term" value="C:amyloplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009011">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (ADP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010021">
        <property type="term" value="P:amylopectin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009960">
        <property type="term" value="P:endosperm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019252">
        <property type="term" value="P:starch biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000025">
        <property type="entry name" value="Starch synthase, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000048">
        <property type="entry name" value="Starch synthase, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02095">
        <property type="entry name" value="glgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825:SF11">
        <property type="entry name" value="ALPHA AMYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825">
        <property type="entry name" value="GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="4 11" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="6 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Starch synthase catalytic" evidence="14">
        <location>
            <begin position="150"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="13">
        <location>
            <begin position="460"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="50"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK19495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="656" mass="72122" checksum="E6D38ADFE8D4750D" modified="2012-06-13" version="1">MATPGVGLGAACLAAAASVAPRRRADPAAPLARRARLLRRVVRGRCVAELSREGAPAAPSQPPVPRPLPSADQQLPPPVLPSFLAPTEAPAPTPLPLPDADAGVGELGPELDIQGIAEDYIDSTDVAASEQDSEIMDAKEQSQAKVTRSIVFVTGEAAPYAKSGGLGDVCGSLPIALAARGHRVMVVMPRYLNGSSDKNYAKAFYTGKNIKIPCFGGSHEVTYFHEYRDNVDWVFVDHPSYHRPGSLYGDNFGAFGDNQFRYTLLCYAACEAPLILELGGYIYGQNCMFVVNDWHASLVPVLLAAKYRPYGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHALDKGEAVNFLKGAVVTSDRIVTVSQGYSWEVTTAEGGQGLNELLSSRKSVLNGIVNGIDINDWNPTMDKCLPYHYSVDDLSGKAKCKAELQKELGLPIRPDIPLIGFIGRLDYQKGIDLIKLAIPDLMRDDVQFVMLGSGDPVFEGWMRSTESSYKDKFRGWVGFSIPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHETGGLRDTVQTFNPFGKTGEEGTGWAFSPLTVDKMLWALRTAMTTFREHKPSWEGLMKRGMSKDHTWDHAAEQYEQIFEWAFVDQPYVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4G7</accession>
    <name>I1H4G7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100835996</name>
        <name evidence="7" type="ORF">BRADI_1g59570v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561524.1">
        <property type="nucleotide sequence ID" value="XM_003561476.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311470.1">
        <property type="nucleotide sequence ID" value="XM_024455702.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311474.1">
        <property type="nucleotide sequence ID" value="XM_024455706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H4G7"/>
    <dbReference type="STRING" id="15368.I1H4G7"/>
    <dbReference type="EnsemblPlants" id="KQK21235">
        <property type="protein sequence ID" value="KQK21235"/>
        <property type="gene ID" value="BRADI_1g59570v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77224">
        <property type="protein sequence ID" value="PNT77224"/>
        <property type="gene ID" value="BRADI_1g59570v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835996"/>
    <dbReference type="Gramene" id="KQK21235">
        <property type="protein sequence ID" value="KQK21235"/>
        <property type="gene ID" value="BRADI_1g59570v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77224">
        <property type="protein sequence ID" value="PNT77224"/>
        <property type="gene ID" value="BRADI_1g59570v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835996"/>
    <dbReference type="eggNOG" id="KOG0143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010119_15_1_1"/>
    <dbReference type="OMA" id="PESYRWG"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045543">
        <property type="term" value="F:gibberellin 2-beta-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010336">
        <property type="term" value="P:gibberellic acid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045487">
        <property type="term" value="P:gibberellin catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="207"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK21235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="383" mass="42620" checksum="85448F8A77F5B4FA" modified="2012-06-13" version="1">MEDHEYEQDESNPPLLATYKHLLDGEHRRLPLGVVAGDEEDDQCDLPVIDLAPLLLDLESDQQTRNNGGSSSAACRAAMVRAASEWGFFQVTNHGVPQPLLDELHGQQLRAFRRPFHRKLAPGDGDDEDQLLLSPEAYRWGNPTATCLHQLSWSEAYHVPITTPSPETKTTTATRRVIHEVSAAMSKLARRILSVLAAEHGPPPETETTTCFLRLNRYPQAPGSGGGHGHGVLGLCGHTDSDFLTILRQDDHVGGLQLLLDDKDGGRRWRTVRPNPGALTVNVGDLLQAWTNDVYASVEHRVVARPDRERFSVAFFLCPSYDTLIRPPLPGKTTTSQVYRNFTFGEYRSQVREDVRLTGRKVGLPRFRRHVSPSPASLTHQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCW3</accession>
    <name>I1HCW3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aurora kinase</fullName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100832120</name>
        <name evidence="13" type="ORF">BRADI_2g05730v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564724.1">
        <property type="nucleotide sequence ID" value="XM_003564676.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCW3"/>
    <dbReference type="FunCoup" id="I1HCW3">
        <property type="interactions" value="1489"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HCW3"/>
    <dbReference type="EnsemblPlants" id="KQK03127">
        <property type="protein sequence ID" value="KQK03127"/>
        <property type="gene ID" value="BRADI_2g05730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832120"/>
    <dbReference type="Gramene" id="KQK03127">
        <property type="protein sequence ID" value="KQK03127"/>
        <property type="gene ID" value="BRADI_2g05730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832120"/>
    <dbReference type="eggNOG" id="KOG0580">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="OMA" id="KIMNLMF"/>
    <dbReference type="OrthoDB" id="377346at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000599">
        <property type="entry name" value="Serine/threonine-protein kinase Aurora-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="28"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="155"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK03127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="291" mass="33926" checksum="36841C28625127E7" modified="2012-06-13" version="1">MAISTDSRSEEKAAQHSQEEKRWVLSDFEVGKALGRGKFGHVYLAREKRSNQIVALKVLFKSQLKQSQVEHQLRREVEIQSHLRHPNILRLYGYFYDQTRVYLILEYAAKGELYKELQRCKHFSERRSATYIASLARALIYLHGKHVIHRDIKPENLLIGVQGELKIADFGWSVHTFNRRRTMCGTLDYLPPEMVEKTEHDYHVDIWSLGILCYEFLYGLPPFEAKEHSETYRRIVKVDLKFPAKPFVSPAAKDLISQMLVKNSAHRLPLNKVLEHPWIVQNADPSGVYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1HD01</accession>
    <name>I1HD01_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS5c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">30S ribosomal protein S5, chloroplastic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100841719</name>
        <name evidence="12" type="ORF">BRADI_2g06080v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565012.1">
        <property type="nucleotide sequence ID" value="XM_003564964.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HD01"/>
    <dbReference type="FunCoup" id="I1HD01">
        <property type="interactions" value="679"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HD01"/>
    <dbReference type="EnsemblPlants" id="KQK03180">
        <property type="protein sequence ID" value="KQK03180"/>
        <property type="gene ID" value="BRADI_2g06080v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841719"/>
    <dbReference type="Gramene" id="KQK03180">
        <property type="protein sequence ID" value="KQK03180"/>
        <property type="gene ID" value="BRADI_2g06080v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841719"/>
    <dbReference type="eggNOG" id="KOG0877">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075168_0_0_1"/>
    <dbReference type="OMA" id="MRQFREV"/>
    <dbReference type="OrthoDB" id="309483at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000001">
        <property type="entry name" value="30S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000002">
        <property type="entry name" value="30S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_B">
        <property type="entry name" value="Ribosomal_uS5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005712">
        <property type="entry name" value="Ribosomal_uS5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01021">
        <property type="entry name" value="rpsE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF61">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US5M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="11">
        <location>
            <begin position="168"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="52"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK03180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="324" mass="34485" checksum="79995D356974B296" modified="2012-06-13" version="1">MAATATATAMATATAPPAAPVVTALFSSLPQRLRFCPKPLLSTSRRLVLPVPKSSSWDGAASDQEDVESEAEAVSGEDAEEDEDGDKKPLAEPVSSSEFQFAAPPEGYVEPAPFDELPPESPDDVTAAYEALYGPAFSGVSLMGNNVFEVKVVDPVDVDRDQRPNDDFTERVVQVNRVTKVVKGGRQLSYRAIVVVGDMKGHVGVGVGKAKEVTEAITKAGMNGRRNLVTVPLTKYNTFPHRASADYGAAKVMLRPACPGSGVTAGGAVRIVLEMAGVENALGKQLRSKNPLNNARATVKATQMMRQFSDVAAERGLPMEELWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HE42</accession>
    <name>I1HE42_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100841926</name>
        <name evidence="2" type="ORF">BRADI_2g09407v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565610.1">
        <property type="nucleotide sequence ID" value="XM_003565562.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HE42"/>
    <dbReference type="STRING" id="15368.I1HE42"/>
    <dbReference type="EnsemblPlants" id="KQK03704">
        <property type="protein sequence ID" value="KQK03704"/>
        <property type="gene ID" value="BRADI_2g09407v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841926"/>
    <dbReference type="Gramene" id="KQK03704">
        <property type="protein sequence ID" value="KQK03704"/>
        <property type="gene ID" value="BRADI_2g09407v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841926"/>
    <dbReference type="eggNOG" id="ENOG502SQ4G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_570383_0_0_1"/>
    <dbReference type="OMA" id="RNIWAST"/>
    <dbReference type="OrthoDB" id="673865at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF65">
        <property type="entry name" value="FBD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="10"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="522" mass="57621" checksum="B37010B5FD9F83FC" modified="2012-06-13" version="1">MAAGAADEEEDRLSGLPDDVLHSILARLPFKHAVRTSALSRRWPRLWLHALAASPVLDFTDPDFVRGHSRAQFVATVNRCLSARGAAPLRALRVAFSPFGAFERDVVGWISAALGSGATEVDVDLRAQGRRRSGGIEADAEDDGGGTFRRTEVELRGDLFCSPSSLSRLSLSRFSLRHVPPGAAGLAGLTSLSLSHVDVTDDALRDAVSGCPVLEFLSLRSCDLLSNVRIAGGRLRGLEVVRCQVRQLQVAAPALESFAFHGDLMYLREDDSKPLEFIGSKGNNVTPPPSEATPELRDAYLSHVDFSGEDEVFDGFAYTEFLNKVAHAKILTICSVALLHIEEERFFSMPTIDTPNLQELQVLMGSLGDDDDTRFPDFFELAAPPLLERLFIQLPADCDGDSEEEDTDMVPGHEISLGRLTFIKVANFRGTRRELRLLRFLVGMAPALEQLVLVTPDPEAEQGGTTLRHDDQDKQQLLQIVQEQLSEVRKARRDQAHVVVCRARDDGSRRPAHSKYYHHDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HM69</accession>
    <name>I1HM69_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prokaryotic-type class I peptide chain release factors domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100841223</name>
        <name evidence="6" type="ORF">BRADI_2g36930v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568985.1">
        <property type="nucleotide sequence ID" value="XM_003568937.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HM69"/>
    <dbReference type="STRING" id="15368.I1HM69"/>
    <dbReference type="EnsemblPlants" id="KQK07674">
        <property type="protein sequence ID" value="KQK07674"/>
        <property type="gene ID" value="BRADI_2g36930v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841223"/>
    <dbReference type="Gramene" id="KQK07674">
        <property type="protein sequence ID" value="KQK07674"/>
        <property type="gene ID" value="BRADI_2g36930v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841223"/>
    <dbReference type="eggNOG" id="KOG2726">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_0_2_1"/>
    <dbReference type="OMA" id="ECQQSRS"/>
    <dbReference type="OrthoDB" id="2019491at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HM69">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1660:FF:000002">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00093">
        <property type="entry name" value="Rel_fac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001859">
        <property type="entry name" value="PRK00591.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF7">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="5">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="100"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK07674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="417" mass="46752" checksum="E73D733A1214BF0E" modified="2012-06-13" version="1">MQHQIRRCGGVAFAALRRVGHFPAAAAAAAATPAAWRRLTSPRLYSTADTSQQLPANLVQIMEQRMKLIEQRSAYLQEQINQPAASPEEYSRANKEFHKLESTMELIKELQSKQKEIEGLKSLVTNSVEEKDMREMAAEELLEAIEEEKQLQHGLFRTLLPKDEADERDCILEVRAGTGGEEASLFAMDIFKMYEKYSQKNGWKFDVLDLMESVSKGYKEASGTISGPGAYGKLKFESGVHRVQRVPVTEKSGRVHTSAVSVAVLPQADEVDVQLRNEDLRIDTYRSGGSGGQSVNTTDSAVRITHVPTGTVVAIQDERSQHQNKAKALKVLRARLYEMERHRLHADRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRVGITHHSIEDVMEGESLDVFIDALLLQEEMDAIASFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HN73</accession>
    <name>I1HN73_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1664 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100831231</name>
        <name evidence="3" type="ORF">BRADI_2g39880v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231872.1">
        <property type="nucleotide sequence ID" value="XM_010233570.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HN73"/>
    <dbReference type="EnsemblPlants" id="KQK08137">
        <property type="protein sequence ID" value="KQK08137"/>
        <property type="gene ID" value="BRADI_2g39880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831231"/>
    <dbReference type="Gramene" id="KQK08137">
        <property type="protein sequence ID" value="KQK08137"/>
        <property type="gene ID" value="BRADI_2g39880v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831231"/>
    <dbReference type="eggNOG" id="ENOG502R402">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 2"/>
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    <dbReference type="ExpressionAtlas" id="I1HN73">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012458">
        <property type="entry name" value="DUF1664"/>
    </dbReference>
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        <property type="entry name" value="OS01G0112300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47289">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE (DUF1664)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07889">
        <property type="entry name" value="DUF1664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1664" evidence="2">
        <location>
            <begin position="89"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK08137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="309" mass="33497" checksum="A662E5D1046C7C39" modified="2012-06-13" version="1">MVLGNIAILLGSGYLGTILTSDEGSKVPILGGLLSGAAKFVSQDGKEASSNNDQHTAQLMSQVDRLREDLRRHLGCREVTVVTTRSSGPGALTITAVVAAGVIGYAYIRWKGWKVSDFMWVTKRGLSDACNVVGNQLTEVSDSVHVAKKHLSGRIDRVDASLDEAQEIIEGTRDEVAIIHEDLSTFQRELQEVNHTVQIWGSRLSSIEDTQDRTVRATEALVGFGQQMEHDQNANIRQVSSFLPAPGPSEQNSKRQLPPPPPLAVPTVPSVAKSKPTAEESQEKHGASPEASMRWKLPGLSFLRTSSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQ11</accession>
    <name>I1HQ11_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100840424</name>
        <name evidence="8" type="ORF">BRADI_2g45530v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569485.1">
        <property type="nucleotide sequence ID" value="XM_003569437.4"/>
    </dbReference>
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    <dbReference type="FunCoup" id="I1HQ11">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HQ11"/>
    <dbReference type="EnsemblPlants" id="KQK09018">
        <property type="protein sequence ID" value="KQK09018"/>
        <property type="gene ID" value="BRADI_2g45530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840424"/>
    <dbReference type="Gramene" id="KQK09018">
        <property type="protein sequence ID" value="KQK09018"/>
        <property type="gene ID" value="BRADI_2g45530v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840424"/>
    <dbReference type="eggNOG" id="ENOG502R7AV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062946_1_0_1"/>
    <dbReference type="OMA" id="FWDFEPE"/>
    <dbReference type="OrthoDB" id="777519at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF140">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="98"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="151"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK09018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="300" mass="31945" checksum="749A5C839909DA45" modified="2012-06-13" version="1">MSRSRTVRIFWDDPDVTDSSGEEESCGGARRVARMVRELPPAPAPALLVQAGFTSAGFPEQCILGEDDDQARPVGGACTGARKRSAKGGSAAAAGTNKFRGVRRRPWGKYAAEIRDPWRGVRVWLGTFDTAEEAARVYDSAAIQLRGASATTNFSTSSEGATQDPAATGYESGAESSQAVSSPTSVLRKVPSMSSLAEDRGDDSEAYQGDNGGSSSLAVLEELGEFVPFEDAPVYSSSSFWDFEPQAGFLYAEPSSPDEASWGDASAGEPWAAAPVQDNNDYFFQDLRDLFPLNPPPAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQE0</accession>
    <name>I1HQE0_BRADI</name>
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        <recommendedName>
            <fullName evidence="11">60S ribosomal protein L37a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100838292</name>
        <name evidence="7" type="ORF">BRADI_2g17040v3</name>
        <name evidence="8" type="ORF">BRADI_2g46580v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569563.1">
        <property type="nucleotide sequence ID" value="XM_003569515.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQE0"/>
    <dbReference type="SMR" id="I1HQE0"/>
    <dbReference type="STRING" id="15368.I1HQE0"/>
    <dbReference type="EnsemblPlants" id="KQK04962">
        <property type="protein sequence ID" value="KQK04962"/>
        <property type="gene ID" value="BRADI_2g17040v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09189">
        <property type="protein sequence ID" value="KQK09189"/>
        <property type="gene ID" value="BRADI_2g46580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838292"/>
    <dbReference type="Gramene" id="KQK04962">
        <property type="protein sequence ID" value="KQK04962"/>
        <property type="gene ID" value="BRADI_2g17040v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09189">
        <property type="protein sequence ID" value="KQK09189"/>
        <property type="gene ID" value="BRADI_2g46580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831339"/>
    <dbReference type="KEGG" id="bdi:100838292"/>
    <dbReference type="eggNOG" id="KOG0402">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141199_1_0_1"/>
    <dbReference type="OMA" id="GPRYGRK"/>
    <dbReference type="OrthoDB" id="1842026at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000002">
        <property type="entry name" value="60S ribosomal protein L37a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48149">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48149:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK04962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK09189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="10243" checksum="EBBC9B226FE3E3DB" modified="2012-06-13" version="1">MTKRTKKAGIVGKYGTRYGASLRKQIKKMEVSQHSKYFCEFCGKFAVKRKAVGIWGCKDCGKVKAGGAYTMNTASAVTVRSTIRRLREQTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQE3</accession>
    <name>I1HQE3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TAFII28-like protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100838901</name>
        <name evidence="10" type="ORF">BRADI_2g46610v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TAFs are components of the transcription factor IID (TFIID) complex that is essential for mediating regulation of RNA polymerase transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569565.1">
        <property type="nucleotide sequence ID" value="XM_003569517.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQE3"/>
    <dbReference type="FunCoup" id="I1HQE3">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HQE3"/>
    <dbReference type="EnsemblPlants" id="KQK09195">
        <property type="protein sequence ID" value="KQK09195"/>
        <property type="gene ID" value="BRADI_2g46610v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838901"/>
    <dbReference type="Gramene" id="KQK09195">
        <property type="protein sequence ID" value="KQK09195"/>
        <property type="gene ID" value="BRADI_2g46610v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838901"/>
    <dbReference type="eggNOG" id="KOG3219">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088696_1_0_1"/>
    <dbReference type="OMA" id="MQRYEVF"/>
    <dbReference type="OrthoDB" id="28335at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08048">
        <property type="entry name" value="HFD_TAF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000055">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045127">
        <property type="entry name" value="TAF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006809">
        <property type="entry name" value="TAFII28_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04719">
        <property type="entry name" value="TAFII28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII28-like protein" evidence="9">
        <location>
            <begin position="114"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK09195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="216" mass="23818" checksum="A0D6FF10A6B9DD49" modified="2012-06-13" version="1">MKDPFEAAVEEQESPPDSPAQPEEDAAAEAPHAISEDYDGGAASGGPRAPPPRPQPSSHAGPSTSVAPALPRTKVRHQKEQDDDDDEEDHMEVDLDKLPSGTSDPDKLAKMNALLSQFTEDQMNRYESFRRSGFQKSNMKKLLASITGSQKISMPTTIVVSGIAKMFVGEVIETARIVMTERKDSGPIRPCHIREAYRRLKLEGKIPKRSVPRLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HT60</accession>
    <name>I1HT60_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100834088</name>
        <name evidence="10" type="ORF">BRADI_2g54320v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564559.1">
        <property type="nucleotide sequence ID" value="XM_003564511.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HT60"/>
    <dbReference type="EnsemblPlants" id="KQK10475">
        <property type="protein sequence ID" value="KQK10475"/>
        <property type="gene ID" value="BRADI_2g54320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834088"/>
    <dbReference type="Gramene" id="KQK10475">
        <property type="protein sequence ID" value="KQK10475"/>
        <property type="gene ID" value="BRADI_2g54320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834088"/>
    <dbReference type="eggNOG" id="KOG2569">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020277_4_0_1"/>
    <dbReference type="OMA" id="YVLVIGY"/>
    <dbReference type="OrthoDB" id="29657at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054103">
        <property type="entry name" value="CAND6-7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF2">
        <property type="entry name" value="RE59932P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21904">
        <property type="entry name" value="CAND6-7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094800" evidence="7">
        <location>
            <begin position="25"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="CAND6/7 N-terminal" evidence="9">
        <location>
            <begin position="31"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="8">
        <location>
            <begin position="179"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK10475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="446" mass="50189" checksum="847D8DA787410B14" modified="2012-06-13" version="1">MASAAARLLLAVVVAAAFLRPAAAEIKQESFKDDSRDVILFEKFGFSPRGSVSISLTGASASSKLAKPDPTQLGFFILSDQSLFEAIYQQPPPTEQNPNPDPNTGCVLSSPYVIPLFNFADLDASGNYKHTFPISHPDEYTLFFANCAPQTSVTMGVRTDMYNTNLDGTKDYLSVGQAPVPTIYAFFAFGYVVFLAVWLYVTLYHNRLSAHRIHHLMSCLLLARMLYCISAAEDQHYIRIAGTSHGWDVMFYLFQLVKGVILFAVIALIGTGWSFLKPFLQDKEKKVLMIVIPLQVAANIAAAVVGETGPFLQEWVTWNQIFLFVDVACCCAVLFPVVWSMRSLRESSKTDGKAARTLAKLTLFRQFYVVVIGYLYFTRIIVYALKTITNYKFRWVSVAAEEVATMAFYIFMFYMFKPAERNQYFALDDEEEEAAELALREEEFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HTQ6</accession>
    <name>I1HTQ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100823859</name>
        <name evidence="6" type="ORF">BRADI_2g56011v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564695.1">
        <property type="nucleotide sequence ID" value="XM_003564647.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HTQ6"/>
    <dbReference type="STRING" id="15368.I1HTQ6"/>
    <dbReference type="EnsemblPlants" id="PNT73249">
        <property type="protein sequence ID" value="PNT73249"/>
        <property type="gene ID" value="BRADI_2g56011v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823859"/>
    <dbReference type="Gramene" id="PNT73249">
        <property type="protein sequence ID" value="PNT73249"/>
        <property type="gene ID" value="BRADI_2g56011v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823859"/>
    <dbReference type="eggNOG" id="KOG1745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078295_4_0_1"/>
    <dbReference type="OrthoDB" id="1861411at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000001">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="5">
        <location>
            <begin position="45"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT73249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="137" mass="15596" checksum="25123F8176480E79" modified="2012-06-13" version="1">MARTKQATRMSTNGKVPRKQPVAKKVARKSALAINRVKKPHRFRPGTVALREIRVYQKITEMLICKLPFQRLVREIVQDIKIDIRFQSSAVSALQEAGEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARCIRGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HW83</accession>
    <name>I1HW83_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">hydroperoxide dehydratase</fullName>
            <ecNumber evidence="15">4.2.1.92</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100831133</name>
        <name evidence="17" type="ORF">BRADI_3g01110v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the biosynthesis of jasmonic acid, a growth regulator that is implicated also as a signaling molecule in plant defense. Converts 13-hydroperoxylinolenic acid to 12,13-epoxylinolenic acid.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Lipid metabolism; oxylipin biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Plant hormone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.92" evidence="15"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570794.1">
        <property type="nucleotide sequence ID" value="XM_003570746.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HW83"/>
    <dbReference type="STRING" id="15368.I1HW83"/>
    <dbReference type="EnsemblPlants" id="KQJ92851">
        <property type="protein sequence ID" value="KQJ92851"/>
        <property type="gene ID" value="BRADI_3g01110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831133"/>
    <dbReference type="Gramene" id="KQJ92851">
        <property type="protein sequence ID" value="KQJ92851"/>
        <property type="gene ID" value="BRADI_3g01110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831133"/>
    <dbReference type="eggNOG" id="ENOG502QQNS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045757_0_0_1"/>
    <dbReference type="OMA" id="QCAAKDY"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009978">
        <property type="term" value="F:allene oxide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11071">
        <property type="entry name" value="CYP74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000024">
        <property type="entry name" value="Allene oxide synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF49">
        <property type="entry name" value="INACTIVE LINOLENATE HYDROPEROXIDE LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ92851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="480" mass="52827" checksum="223AB813F0AA2325" modified="2012-06-13" version="1">MLPSFSPAATAAQPRRIPGSHGPPILGPLKDRLDYFWFQGPDEFFRRRAASHKSTVFRANIPPTFPFFLGVDPRVVAVVDAAAFTALFDSSLVDKRDCLIGPYNPSAAFTGGTRVGVYLDTAEPDHARTKAFAMDLLRRSSKIWADEFLSGLDSMISNLESELNNGAAAASYLVPLQQCVFKFLCKAVVGADPAMDKLVDRFGFFILDLWLGLQLIPTQKIGALPQPLEELLFHSFPFPAFLIRPGYDVLYRFVSKHGADAVAVGVDTHGLSQKDAINNILFVLGFNAFGGFSVFLPFLILEIGAKTPTGVNLRPKLREEVRRVLDENNGAIGFGAVKGMPLVRSTVYEVLRTRPPVPLQFGRARENFTLRSHGSEGFAVAAGEMLCGYQPLAMRDPAVFDRPEEFVPDRFVGEEGEKLLKHVYWSNGPETADPAVGNKQCAAKDAVVATACMLVAEMFRRYDDFECEGTSFTKLQKRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYW3</accession>
    <name>I1HYW3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxisomal membrane protein PMP22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100841647</name>
        <name evidence="7" type="ORF">BRADI_3g08480v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571763.1">
        <property type="nucleotide sequence ID" value="XM_003571715.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYW3"/>
    <dbReference type="STRING" id="15368.I1HYW3"/>
    <dbReference type="EnsemblPlants" id="KQJ94097">
        <property type="protein sequence ID" value="KQJ94097"/>
        <property type="gene ID" value="BRADI_3g08480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841647"/>
    <dbReference type="Gramene" id="KQJ94097">
        <property type="protein sequence ID" value="KQJ94097"/>
        <property type="gene ID" value="BRADI_3g08480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841647"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="KMAIYGA"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HYW3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF86">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ94097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22701" checksum="76DB1E29E0818F1E" modified="2012-06-13" version="1">MAGGVAGGGGGGGRGDSLPQRAWRQYLLQLRLHPLRTKMITAGCLAGVSDSVAQKLSGYQKIEKRRLLLKMIFGFAYGGPFGHFLHKVLDYIFKGKKDTKTVAKKVLLEQITSSPWNNILFLFYYGYVVERRPFKEVKTRVRKQYPSVQLSAWMFWPIVGWINHQYVPLQFRVIVHSFVACCWGIFLNLRARAMSLKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2B3</accession>
    <name>I1I2B3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ADP-ribosylation factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100825312</name>
        <name evidence="15" type="ORF">BRADI_3g19000v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234516.1">
        <property type="nucleotide sequence ID" value="XM_010236214.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2B3"/>
    <dbReference type="STRING" id="15368.I1I2B3"/>
    <dbReference type="EnsemblPlants" id="KQJ95788">
        <property type="protein sequence ID" value="KQJ95788"/>
        <property type="gene ID" value="BRADI_3g19000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825312"/>
    <dbReference type="Gramene" id="KQJ95788">
        <property type="protein sequence ID" value="KQJ95788"/>
        <property type="gene ID" value="BRADI_3g19000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825312"/>
    <dbReference type="eggNOG" id="KOG0070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_9_3_1"/>
    <dbReference type="OMA" id="MGAGMSW"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04151">
        <property type="entry name" value="Arl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000510">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQJ95788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20342" checksum="441D61992C4588A1" modified="2012-06-13" version="1">MGIVFTRLFSSVFGNREARILVLGLDNAGKTTILYRLQMGEVVSTIPTIGFNVETVQYNNIKFQVWDLGGQTSIRPYWRCYFPNTQAIIYVVDSSDTDRLVTAKEEFHSILEEDELKGAVVLVYANKQDLPGALDDAAITESLELHKIKSRQWAIFKTSAIKGEGLFEGLDWLSNALKSGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2R9</accession>
    <name>I1I2R9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100828410</name>
        <name evidence="6" type="ORF">BRADI_3g20450v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SEC8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573690.1">
        <property type="nucleotide sequence ID" value="XM_003573642.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2R9"/>
    <dbReference type="FunCoup" id="I1I2R9">
        <property type="interactions" value="2666"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I2R9"/>
    <dbReference type="EnsemblPlants" id="KQJ96022">
        <property type="protein sequence ID" value="KQJ96022"/>
        <property type="gene ID" value="BRADI_3g20450v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828410"/>
    <dbReference type="Gramene" id="KQJ96022">
        <property type="protein sequence ID" value="KQJ96022"/>
        <property type="gene ID" value="BRADI_3g20450v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828410"/>
    <dbReference type="eggNOG" id="ENOG502QRJ3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009514_0_0_1"/>
    <dbReference type="OMA" id="HMEVRCR"/>
    <dbReference type="OrthoDB" id="272977at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="5">
        <location>
            <begin position="21"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="233"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="831"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="233"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="831"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ96022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1052" mass="116527" checksum="403EF6BB40A7E1AC" modified="2012-06-13" version="1">MSRGRRGLFDGLPIPADKSYLKEGLSRIDEGWAAARFDSLPHVVHILTSKDREGEIQFLKEQSELIEDVVDEVVHAYHHGFNKAIQNYSQILRLFSESAESITGLKGEMAEAKKLLGRKNKHLGQLWYRSLTLRHVISLLDQVEDVAKVPARIENLMAEKQLYAAVQLHVQSMLILEREGLQAVGALQDVRSDLTKLRGALFYKILEELHCHLYNNGEYSLVTLNMVDSEEVPTSSATGRLGNSMQPLSRRTRSIKGENHFGGPAIADGIPKSNSVDGGSSFDGPDDDSSLDGRGQKNSKSVSREIPIFLSFATPDEFLESMIKADAPLNVKYLRTLVQCLSMLRKIAAAGAVICQRVRPTIHDVITSKIKAYAGEASKSNIDKVVNRTTSDVSNSHGPVARYQPKQKTKNTASVMATQLVASPISPAMAPTGDAQRAASQLLGSIFECLVDILENHITVGELLEQKSSNEVDNLSTPHISNGDVSWNPDSEFSQATGGFSVAFSLSVVQSECQQLLCEILRATPEAATADAAVQTARLANKDPAKEKRDGSEGLSFAFRVTDAAMSAQNEGQGWRRNSTVPQEGYGTASVIPDQGIFLAASVYRPVFEFMNKIGLMLPQKYSQLGNEGLLTFVNNFLKEHFLPAIFVDYRKCVQQAISSPAAFRPRVNATSVYSSLVDNGRPVLQGLLAVDIIAKEVLGWVQLMPNYASELVEYVRTFLERTHERCRASYMEAVLEKQSYILLSRSDVESLMRLDPANASLQNSFGELDNNIPEAGVEVEIELSDVLLDMCPIKQENLIHDDQKLILLASLSDSLEYLADSVERLGESFISPSTTSESNNHVHQGRHTRSTSAIPKSLASLANEYRRLAIDCVRVLRLEMQLETIYHMQEMTKREYVEDQDAEDPDDFIISLTTQIARRDEEMAPYIAESKRNYIFGGISSVAANASIKALAQMKSINLLGVQQICRNSIALEQALAAIPSIDSEAVQQRLDRVRTFYELLNLPFESLLGFIAEQEYLFSAKEYLSVLKVNVPGREMPMDAERRISQILGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1I511</accession>
    <name>I1I511_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100830038</name>
        <name evidence="13" type="ORF">BRADI_3g29780v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Aquaporins facilitate the transport of water and small neutral solutes across cell membranes. May be involved in transport from the vacuolar compartment to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC 1.A.8.10) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574110.1">
        <property type="nucleotide sequence ID" value="XM_003574062.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I511"/>
    <dbReference type="SMR" id="I1I511"/>
    <dbReference type="STRING" id="15368.I1I511"/>
    <dbReference type="EnsemblPlants" id="KQJ97261">
        <property type="protein sequence ID" value="KQJ97261"/>
        <property type="gene ID" value="BRADI_3g29780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830038"/>
    <dbReference type="Gramene" id="KQJ97261">
        <property type="protein sequence ID" value="KQJ97261"/>
        <property type="gene ID" value="BRADI_3g29780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830038"/>
    <dbReference type="eggNOG" id="KOG0223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_3_4_1"/>
    <dbReference type="OMA" id="LALNTMH"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006833">
        <property type="term" value="P:water transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000002">
        <property type="entry name" value="Probable aquaporin TIP1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF23">
        <property type="entry name" value="AQUAPORIN TIP3-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ97261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="265" mass="27588" checksum="81FE5838ED657DD4" modified="2012-06-13" version="1">MSTAARSTAGRRGFTVGRSEDATHPDTIRAAISEFLATAIFVFAAEGSILSLGKLYHDMSTAGGLVAVALAHALALAVAVSVAVNISGGHVNPAITFGALLGGRITLVRALFYWVAQLLGAIVASLLLRLTTGGMRPPGFSLAAGVRDWHAVLLEAVMTFGLMYAYYATVMDPKRGSVGTIGPLAVGFLLGANMLAGGPFDGAGMNPARVFGPALVGWRWGHHWVYWVGPFLGAGIAGLLYEYLVIPASETAAAHTHQPLAPEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6W2</accession>
    <name>I1I6W2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100841745</name>
        <name evidence="10" type="ORF">BRADI_3g35390v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family. CPR flippase (TC 3.A.1.211) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572173.1">
        <property type="nucleotide sequence ID" value="XM_003572125.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6W2"/>
    <dbReference type="FunCoup" id="I1I6W2">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ98195">
        <property type="protein sequence ID" value="KQJ98195"/>
        <property type="gene ID" value="BRADI_3g35390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841745"/>
    <dbReference type="Gramene" id="KQJ98195">
        <property type="protein sequence ID" value="KQJ98195"/>
        <property type="gene ID" value="BRADI_3g35390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841745"/>
    <dbReference type="eggNOG" id="KOG0059">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_19_5_1"/>
    <dbReference type="OMA" id="GTKGMEW"/>
    <dbReference type="OrthoDB" id="8061355at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I6W2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000633">
        <property type="entry name" value="ABC transporter A family member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF259">
        <property type="entry name" value="OS08G0398300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24526">
        <property type="entry name" value="ABCA12_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="632"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ98195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="954" mass="106178" checksum="EEB9A81F7C7073F4" modified="2012-06-13" version="1">MDSSTGSRGPATFSTQANALLRKNLCFQKRNLKTNVCITLFPILLCVLLVALQGAIDREIDKPKYRCGCACVDTAADGSCRRTECGIQYSTLDQVASCPIPNPPRWPAVVQVPRPESRAIRTASQPFDGLPDPTCRDNGSCPAAFLITGKNRSFAESLSAELFPTLSPSLNFTDYLDVLSKIVPGSDTWTSFRQLLEPTFVPGNTLYIVQPQCRSNLSQTISVNAGPIPLQLNVDCIEGLSLWRESASVINDELFKGYRQQRESGGGKTNEFAAGYDFLNTNKDSLDLSIWFNSTYNNNTAYSNIALLRVPRLVNMVSNSYIKFLRGSGVAMLLEYVKDMPKVGTKFKFDLSSLIGALFFTWIIELLFPVVLTYLVYEKQQKLKIMMKMHGLKDGPYWMITYAYFLALSVVYMILFVIFGSLIGLRFFTNNDYSIQFAFYFIYINLQIALAFFAASFFSSVKIATVVGYIYVFGSGLLGEFLLRFFVEDNGFPKGWIVVMELIPGFSLFRGLYELGQYSFSGNAMGTNGMKWSNLSDPVNGMRTVLIIMVVEWAILLPLAFYLDQVSLSGGGYRKRFLLFLKCFKKRAVSFRRHSFGRIGSKVVVEMENPDSTQEREVVEQLLLEPNANHAIICDNLKKVYHGKDGNPDKLAVRGLSLALPKGQCFGMLGPNGAGKTSFISMMIGLIPPTSGTAYVHGMDIRTDMNDIYANMGVCPQHDLLWETLTGREHLLFYGRLKNLKGAELLKATDDSLKSVNLFHGGVGDKQVGKYSGGMKRRLSVAISLIGDPKVVFMDEPSTGLDPASRNNLWSVVKEAKKNRAIILTTHSMEEAEVLCDRLGIFVDGGFQCIANPKELKARYGGTYVFTMTTASEHEQEVEHLVHRLSPSANRIYHISGTQKFELPKQEVKIADVFHEVESAKCRFSIHAWGLADTTLEDVFIKVAKGAEAFSVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8J0</accession>
    <name>I1I8J0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100826576</name>
        <name evidence="3" type="ORF">BRADI_3g40221v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574765.1">
        <property type="nucleotide sequence ID" value="XM_003574717.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8J0"/>
    <dbReference type="STRING" id="15368.I1I8J0"/>
    <dbReference type="EnsemblPlants" id="PNT68406">
        <property type="protein sequence ID" value="PNT68406"/>
        <property type="gene ID" value="BRADI_3g40221v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68407">
        <property type="protein sequence ID" value="PNT68407"/>
        <property type="gene ID" value="BRADI_3g40221v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826576"/>
    <dbReference type="Gramene" id="PNT68406">
        <property type="protein sequence ID" value="PNT68406"/>
        <property type="gene ID" value="BRADI_3g40221v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68407">
        <property type="protein sequence ID" value="PNT68407"/>
        <property type="gene ID" value="BRADI_3g40221v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826576"/>
    <dbReference type="eggNOG" id="ENOG502S6A4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121625_1_0_1"/>
    <dbReference type="OMA" id="WIKPERE"/>
    <dbReference type="OrthoDB" id="746946at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37213">
        <property type="entry name" value="SUBTILISIN-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37213:SF1">
        <property type="entry name" value="SUBTILISIN-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT68407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="100" mass="11066" checksum="CE799F872D6FDA1C" modified="2012-06-13" version="1">MVPGRGWSRTAGNARSFVGNALGGVRGWSNLASWAVAGTLAYYLWVRPARQLQKEQQERAALASASDPYRYVEKRKPIPDPQDTGLIYGKKKDDPTKSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBC6</accession>
    <name>I1IBC6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_3g48097v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBC6"/>
    <dbReference type="FunCoup" id="I1IBC6">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00237">
        <property type="protein sequence ID" value="KQK00237"/>
        <property type="gene ID" value="BRADI_3g48097v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00237">
        <property type="protein sequence ID" value="KQK00237"/>
        <property type="gene ID" value="BRADI_3g48097v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830838"/>
    <dbReference type="eggNOG" id="ENOG502QQUR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015101_2_1_1"/>
    <dbReference type="OMA" id="VAGPCHA"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF666">
        <property type="entry name" value="OS02G0608801 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095236" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK00237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="42941" checksum="F2C87C268D866001" modified="2012-06-13" version="1">MANTATIIACIVVALYLVSPCQARPLPQPPAPSPNQGIVDGITAIFNFGDSISDTGNFIREGAVGMMRHTGVLPYGSAIADGATGRCSDGYLMIDNLAKDLGLPLLKPYLDKGADFTHGVNFAVTGSTALTTAALARRGITVPHTNSSLDVQLKWFKDFMAATTKSPQEIRDKLGSSLVLMGEIGGNDYNYAFVTNKPAAAAEGSIYNAIRTTVGAVEAMALVPEVVQSVLDAARELLEMGATRMVIPGNFPVGCVPSYLSAVDEKDPAAYDGNGCLIGLNFFAQMHNVALQRGIRELRGAYPDATISYADYFSAYVRLLRDAGRMGFDSAAATKACCGVGRGAYNVDMDRMCGAPGTTVCARPNEYVSWDGVHLTQHAYKVLSDLLYHGGLASPAPVNFTETRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBF6</accession>
    <name>I1IBF6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100844395</name>
        <name evidence="13" type="ORF">BRADI_3g48360v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575327.1">
        <property type="nucleotide sequence ID" value="XM_003575279.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBF6"/>
    <dbReference type="FunCoup" id="I1IBF6">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00273">
        <property type="protein sequence ID" value="KQK00273"/>
        <property type="gene ID" value="BRADI_3g48360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844395"/>
    <dbReference type="Gramene" id="KQK00273">
        <property type="protein sequence ID" value="KQK00273"/>
        <property type="gene ID" value="BRADI_3g48360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844395"/>
    <dbReference type="eggNOG" id="KOG0192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_7_35_1"/>
    <dbReference type="OMA" id="CREGNAM"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000211">
        <property type="entry name" value="Integrin-linked protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000355">
        <property type="entry name" value="Integrin-linked protein kinase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000180">
        <property type="entry name" value="serine/threonine-protein kinase STY46-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF7">
        <property type="entry name" value="OS02G0608500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="196"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK00273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="483" mass="53929" checksum="D9E6BB5EEF30CD47" modified="2012-06-13" version="1">MAAAAKLTRNLSRQLSSGAARIWRQLSLEPHTPRRGVGAAAAAGAVAGPTRFGIARQSSLDPTPAAADVAMLAVPDNLDATMRLLFAACQGDVGGVEELLREGVDVDSIDLDGRTALHIASCEGQGEVVRLLLAWKANINARDRWGSTPALDAKHYGHFEVYNLLRARGAILPKSKKTPMVVSNPKEVPEYELNPLELEFRRGEEVTKGYYIAKWYGSKVFVKILDKESFSDCDSIDSFKHELTLLEKARHPNLVQFVGAVTQNVPLMIVSEYHQNGDLASYLETKGRLQSYKAIRFALDIARGLNYLHECKPEPIIHGDLSPKNIVRDDEGTLKVAGFGSFGLIKVSEDKLRMARPVSKFDSVYVAPEIYRNETFDRSVDTFAFGLILYEMIEGTPAFHPKPPEEAAKMICLEGLRPLFKNKPKSYPEDVKELIQECWDTTPSVRPTFSDIIERLNKIYASCSKQTRWRDNFKLPWKQAAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IET4</accession>
    <name>I1IET4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100829500</name>
        <name evidence="8" type="ORF">BRADI_3g57830v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570554.1">
        <property type="nucleotide sequence ID" value="XM_003570506.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IET4"/>
    <dbReference type="FunCoup" id="I1IET4">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01727">
        <property type="protein sequence ID" value="KQK01727"/>
        <property type="gene ID" value="BRADI_3g57830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829500"/>
    <dbReference type="Gramene" id="KQK01727">
        <property type="protein sequence ID" value="KQK01727"/>
        <property type="gene ID" value="BRADI_3g57830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829500"/>
    <dbReference type="eggNOG" id="ENOG502QQ3S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025359_1_1_1"/>
    <dbReference type="OMA" id="SPMNGRD"/>
    <dbReference type="OrthoDB" id="1728340at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IET4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="15"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="188"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK01727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="398" mass="41999" checksum="7285AAD99741341A" modified="2012-06-13" version="1">MGVGRVLNDVKPYLAMVLLQVGFAGMYVVAVASLKGGMSHFVLVVYRNLVATAVMAPFALYFERGQRPPMTIKVFLKIMGLAFLEPVLDQNLYFMGAKLTSASFASALINILPAVTFVLALILRMEKVRLRSLHSQAKIAGTVLTVAGAVLMILYHGPAVQFPWTKGRAGAANHGAGQGQGQGAGWLSGTIMTIAACVAWACFFVLQSSTLQSYPAELSLTVLICAVGSLMSGSVALVAERGNTQAWVIGFDTRLFTAVYAGIVCSGVAYYVQGIVSRQRGPVFVTAFNPLCMIITSVMGSIILKEEITLGSVIGAAIIVAGLYFLIWGKSKDEVSQVPVKGAGELPLTSEISQVSAKGAGEPPLTSVTTDNKGKQEIGNGGHVFDVKKPAAANGLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IFJ1</accession>
    <name>I1IFJ1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100833096</name>
        <name evidence="17" type="ORF">BRADI_3g60011v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell junction</location>
            <location evidence="12">Plasmodesma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cysteine-rich repeat secretory protein family. Plasmodesmata-located proteins (PDLD) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573132.1">
        <property type="nucleotide sequence ID" value="XM_003573084.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IFJ1"/>
    <dbReference type="STRING" id="15368.I1IFJ1"/>
    <dbReference type="EnsemblPlants" id="PNT69689">
        <property type="protein sequence ID" value="PNT69689"/>
        <property type="gene ID" value="BRADI_3g60011v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833096"/>
    <dbReference type="Gramene" id="PNT69689">
        <property type="protein sequence ID" value="PNT69689"/>
        <property type="gene ID" value="BRADI_3g60011v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833096"/>
    <dbReference type="eggNOG" id="ENOG502QWR4">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:plasmodesma"/>
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        <property type="entry name" value="Cysteine-rich repeat secretory protein 15"/>
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        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Cell2Cell_Antifungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
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    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32080">
        <property type="entry name" value="ANTIFUNGAL PROTEIN GINKBILOBIN-2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32080:SF3">
        <property type="entry name" value="PLASMODESMATA-LOCATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GNK2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003644753" description="Gnk2-homologous domain-containing protein" evidence="15">
        <location>
            <begin position="22"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="16">
        <location>
            <begin position="33"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="16">
        <location>
            <begin position="143"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022949"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00024184"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038393"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="PNT69689.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69689"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="275" mass="27465" checksum="65176C2DCE0E3133" modified="2012-06-13" version="1">MAPTRVASFLFLFFLVSGTYTSSVHGAADDDTASFVYAGCSQGRYAAGTQYESGVDSVLTSLANSAPYAPYANTTSPASSAVSAVSGLYQCRSDLASSICTSCVRSAINRLSSLCAWSSGGAVQLRGCFVRYGNDSFVGKPNTAVLFKKCGGGSPDAGAGAMAMRDSALGALASEAAPGYRAGGAGGVQAMAQCVGDLDAKACSECVSAAAAQLKAGCGNAGAGEVYLGKCYARFWSNGGGFVSSGAGNRRLALAVAVAGGFFASSLAYLPAVLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IHH8</accession>
    <name>I1IHH8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100835984</name>
        <name evidence="8" type="ORF">BRADI_4g04650v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579195.1">
        <property type="nucleotide sequence ID" value="XM_003579147.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IHH8"/>
    <dbReference type="STRING" id="15368.I1IHH8"/>
    <dbReference type="EnsemblPlants" id="KQJ86313">
        <property type="protein sequence ID" value="KQJ86313"/>
        <property type="gene ID" value="BRADI_4g04650v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835984"/>
    <dbReference type="Gramene" id="KQJ86313">
        <property type="protein sequence ID" value="KQJ86313"/>
        <property type="gene ID" value="BRADI_4g04650v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835984"/>
    <dbReference type="eggNOG" id="KOG2030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003612_1_0_1"/>
    <dbReference type="OMA" id="MFLEFFA"/>
    <dbReference type="OrthoDB" id="207084at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="AGAP008075-PA-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.310.10:FF:000002">
        <property type="entry name" value="nuclear export mediator factor Nemf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021846">
        <property type="entry name" value="NFACT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11923">
        <property type="entry name" value="NFACT-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="988"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="185"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="745"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="847"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="217"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="745"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="767"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="859"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="883"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="923"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ86313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1163" mass="129791" checksum="C6B95F873C23B752" modified="2012-06-13" version="1">MVKARMTTADVAAEVKCLRRLIGMRLSNVYDITPKTYLFKLMNSSGITESGESEKVLLLMESGVRLHTTQYVRDKSTTPSGFTLKLRKHVRSKRLEDVRMLGYDRMILFQFGLGSNAHFIILELYAQGNIILTDSEYTVMTLLRSHRDDNKGLAIMSRHRYPVEACRTFERTDFTKLKDTLKLSNTVDGEDSSQVTPNSADSHEPSESVNDGVPVTDKLEEPSNRTEKKSAVKIKQPGSNAKASNGTQSNKSTLKTLLGEALAYGPALAEHIILDAGLLPSTKVGKDPESSIDDHTIQSLVESVTRFEDWLVDIISGQRIPEGYILMQNKMSAKKNITPSEVSSTNQKIYDEYCPILLKQFKAREYDEFETFDAALDEFYSKIESQRVNQQQKAKEDSAVQRLNKIKLDQENRVHTLRKEADHCIKMAELIEYNLEDVDAAIVAVRVSLANGMSWEALARMIKEERRAGNPVAGLIDKLSFENNCITLLLSNNLDDMDEDEKTAPVEKVEVDLSLSAHANARRWYEMKKKQETKQEKTITAHDKAFKAAEKKTRLQLAQEKTVAAITHMRKVHWFEKFNWFISSENYLIVSGRDAQQNELVVKRYMSKGDLYVHAELHGASSTIIKNHKPDSPIPPLTLNQAGCFTVCHSKAWDSKIVTSAWWVYPHQVSKTAPTGEYLTVGSFMIRGKKNFLPPHPLVMGFGILFRLDESCLASHLNERRIRGEDEALPEIEVEPWKRHNISELDDKLANDNETSKGIHENESSRDYTSVQQNYDASPSNQPSNMGTASSSEQLSEAQTVENNGVASTFNEETRDDSVSSQLEDLLDKNLGLGPAKVSGKSSLLISSHSSLPEDTDDLDVKKTIQREKPYVSKAERRKLKKGQNSCESTSDPQNGEAVKKPGNSQQEKGKDNTKTANPKTSRGQKGKLKKIKEKYAEQDDEEREIRMALLASSGKASQKGKPSQDGEDTNAKQAKSSTGEVDSVKICYKCKRSGHLSRDCPESTSVVVPTDVNVGRSRDVTDKSASAPVDGSIDMDEDDIHELGDEEKEKLIDLDYLTGIPLPSDILLYAVPVCAPYNALQTYKYRVKITPGTAKKGKAAKTALSLFMHIPDATNREKELMKACTDPELVAAIIGNAKITAPGLTQLKQKQKQKGKKAAKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJJ0</accession>
    <name>I1IJJ0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100836178</name>
        <name evidence="11" type="ORF">BRADI_4g10550v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575711.1">
        <property type="nucleotide sequence ID" value="XM_003575663.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757951.1">
        <property type="nucleotide sequence ID" value="XM_014902465.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJJ0"/>
    <dbReference type="EnsemblPlants" id="KQJ87362">
        <property type="protein sequence ID" value="KQJ87362"/>
        <property type="gene ID" value="BRADI_4g10550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ87363">
        <property type="protein sequence ID" value="KQJ87363"/>
        <property type="gene ID" value="BRADI_4g10550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836178"/>
    <dbReference type="Gramene" id="KQJ87362">
        <property type="protein sequence ID" value="KQJ87362"/>
        <property type="gene ID" value="BRADI_4g10550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87363">
        <property type="protein sequence ID" value="KQJ87363"/>
        <property type="gene ID" value="BRADI_4g10550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836178"/>
    <dbReference type="HOGENOM" id="CLU_000288_158_1_1"/>
    <dbReference type="OMA" id="CENEEFI"/>
    <dbReference type="OrthoDB" id="665057at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21037">
        <property type="entry name" value="MLKL_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000604">
        <property type="entry name" value="Proline-rich receptor-like protein kinase PERK8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059179">
        <property type="entry name" value="MLKL-like_MCAfunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054000">
        <property type="entry name" value="MLKL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27006:SF606">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27006">
        <property type="entry name" value="PROMASTIGOTE SURFACE ANTIGEN PROTEIN PSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22215">
        <property type="entry name" value="MLKL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095313" description="non-specific serine/threonine protein kinase" evidence="9">
        <location>
            <begin position="25"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="190"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ87362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="482" mass="53069" checksum="C2F355AE4CC17A40" modified="2012-06-13" version="1">MVAAAVAAGSALKLIFSLVIAIKAASNKAKRNKDDCRDIASRACSLEASLTSLDDGGGAAAKHPAVASALDRLHDALYRALQAVMECEEDGAVARRFNAGKVSDELRKLNQSIGDRVLDVILATSLHTNSILLGARQSKHLGDERLLLPPLPQIQESRLANSCKEGEPAAASSGLKKFNLSELEVATKNFSKQNLIEESDSCTVYKGELRDGSKVAIKAYSKMQYKACRKECENEEFIAAKLLHKNIVELVGCCSSGGLFYQVYEYMHNRSLSDHLHGSKIEWAKIFNTIIQGIARGIDYLHEQCGLCIVHLHLKPSSILLDHDYTPKICDFGISKILPTSAKEGIVDTVIGTWGFTAPEYMLSHRFSIKSDVYSFGVILLELITGWSRHQEAKNCKDPVNVLVWGLWKAGKLDECVDPRLACATGVTESQVAEMKRCIHVALLCIEEDPALRPDMSDVLRMLRDNSPITDRRSPRRPAYTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJJ2</accession>
    <name>I1IJJ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7792 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836793</name>
        <name evidence="3" type="ORF">BRADI_4g10570v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237440.1">
        <property type="nucleotide sequence ID" value="XM_010239138.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJJ2"/>
    <dbReference type="EnsemblPlants" id="KQJ87366">
        <property type="protein sequence ID" value="KQJ87366"/>
        <property type="gene ID" value="BRADI_4g10570v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836793"/>
    <dbReference type="Gramene" id="KQJ87366">
        <property type="protein sequence ID" value="KQJ87366"/>
        <property type="gene ID" value="BRADI_4g10570v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R4U0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1035647_0_0_1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IJJ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21037">
        <property type="entry name" value="MLKL_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056694">
        <property type="entry name" value="DUF7792"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059179">
        <property type="entry name" value="MLKL-like_MCAfunc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35832">
        <property type="entry name" value="OS12G0248400 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35832:SF9">
        <property type="entry name" value="OS12G0276800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25055">
        <property type="entry name" value="DUF7792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7792" evidence="2">
        <location>
            <begin position="14"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ87366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="276" mass="31132" checksum="A756300DAB7C3CAD" modified="2012-06-13" version="1">MVDLAVFGVGGGVLRLIFTLAVAIGAKADTAKQNREECLRIARRAAKLHGTLAAQAYTLAEATRRHPAVASVLNDLREALREALEGVKACQRDCGALIRLLKAARVSAQLRVLNRDIKDRIMDVLLVTSLYTNGLVYTMHLYHMEHLRIQRHIMMHGHHMEHHRLPRPLPQMQIQLEQHGYGNTHAQSLSNQSYAIQAASPSLPDQRATSSSIPCQIEWRAAVPEDVVDFRREARESNIEPRLVVLLILVLLLLGTRCGLERLLLFYLWRLLFRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1INF2</accession>
    <name>I1INF2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100824846</name>
        <name evidence="8" type="ORF">BRADI_4g25170v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577828.1">
        <property type="nucleotide sequence ID" value="XM_003577780.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INF2"/>
    <dbReference type="FunCoup" id="I1INF2">
        <property type="interactions" value="553"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1INF2"/>
    <dbReference type="EnsemblPlants" id="KQJ89377">
        <property type="protein sequence ID" value="KQJ89377"/>
        <property type="gene ID" value="BRADI_4g25170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824846"/>
    <dbReference type="Gramene" id="KQJ89377">
        <property type="protein sequence ID" value="KQJ89377"/>
        <property type="gene ID" value="BRADI_4g25170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824846"/>
    <dbReference type="eggNOG" id="ENOG502R9U7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038898_2_0_1"/>
    <dbReference type="OMA" id="PWSSCAS"/>
    <dbReference type="OrthoDB" id="2020802at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044800">
        <property type="entry name" value="LEC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR ABI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140:SF133">
        <property type="entry name" value="TF-B3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="7">
        <location>
            <begin position="38"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="142"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ89377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="277" mass="31134" checksum="255EA2390D88C630" modified="2012-06-13" version="1">MAMNPLSQEHHPNAWPWGVAMYTNLHYQQYHYEKEHLFEKALTPSDVGKLNRLVIPKQHAERCFPLGGDSGEKGLLLSFDDEAGKPWRFRYSYWTSSQSYVLTKGWSRYVKEKQLDAGDVVHFERVRGLGTGDRLFIGCRRRGDVGAPTPPPAVRVMPASGQNHPREQHQQQPWSPMCYSTSGSYPTSPANSQAYRRSVDHDHSDMHHAGESQWDADGRTCSPASAPTRRLRLFGVNLDCGPEPEADTSTATEAMYGYMHQIPYAAVSPVPSNWGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1ISC4</accession>
    <name>I1ISC4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836908</name>
        <name evidence="3" type="ORF">BRADI_4g36650v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. Sedlin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578539.1">
        <property type="nucleotide sequence ID" value="XM_003578491.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ISC4"/>
    <dbReference type="STRING" id="15368.I1ISC4"/>
    <dbReference type="EnsemblPlants" id="KQJ91260">
        <property type="protein sequence ID" value="KQJ91260"/>
        <property type="gene ID" value="BRADI_4g36650v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836908"/>
    <dbReference type="Gramene" id="KQJ91260">
        <property type="protein sequence ID" value="KQJ91260"/>
        <property type="gene ID" value="BRADI_4g36650v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836908"/>
    <dbReference type="eggNOG" id="KOG3444">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085828_2_3_1"/>
    <dbReference type="OMA" id="FHYIVHC"/>
    <dbReference type="OrthoDB" id="10258445at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14854">
        <property type="entry name" value="TRAPPC2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044760">
        <property type="entry name" value="TRAPPC2L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ91260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="143" mass="15949" checksum="620DAB996C4C29CC" modified="2012-06-13" version="1">MIVCVAVVGHQNNPLYLQSFTEADDALKLHHVVHCSLDVIDERVNNPKRNAPTLNETFLGLLYPTENYKVYGYLTNTKVKFLMVTTDLDVKDADARNFFRKFHAAYVDAVSNPFHVPGKKIASRSFGARVSTIVKSFGSGTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>I1IWY7</accession>
    <name>I1IWY7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC100844338</name>
        <name evidence="24" type="ORF">BRADI_5g07360v3</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">Bd21</strain>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Protein kinase that acts as a blue light photoreceptor in a signal-transduction pathway for phototropic responses. Regulates a wide range of physiological activities in plants that maximize the efficiency of photosynthesis, such as chloroplast relocations, stomata opening, and leaf expansion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581132.1">
        <property type="nucleotide sequence ID" value="XM_003581084.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239787.1">
        <property type="nucleotide sequence ID" value="XM_010241485.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239788.1">
        <property type="nucleotide sequence ID" value="XM_010241486.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751394.1">
        <property type="nucleotide sequence ID" value="XM_014895908.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IWY7"/>
    <dbReference type="STRING" id="15368.I1IWY7"/>
    <dbReference type="EnsemblPlants" id="KQJ82208">
        <property type="protein sequence ID" value="KQJ82208"/>
        <property type="gene ID" value="BRADI_5g07360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844338"/>
    <dbReference type="Gramene" id="KQJ82208">
        <property type="protein sequence ID" value="KQJ82208"/>
        <property type="gene ID" value="BRADI_5g07360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844338"/>
    <dbReference type="eggNOG" id="ENOG502QPPH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006321_3_0_1"/>
    <dbReference type="OMA" id="THVCLIS"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009882">
        <property type="term" value="F:blue light photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000133">
        <property type="entry name" value="LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000002">
        <property type="entry name" value="LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000265">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000036">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0157">Chromophore</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="22">
        <location>
            <begin position="103"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="23">
        <location>
            <begin position="177"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="22">
        <location>
            <begin position="380"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="23">
        <location>
            <begin position="454"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="581"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="18"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="610"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQJ82208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="909" mass="101370" checksum="23A069E9337351F2" modified="2012-06-13" version="1">MAGAGSSKGMADAVEKWMAFPAGGEEETTGGFTLPMPPLSPPGGKEIVEEPGNQQQQQGWWPKPAAEQRGSVSGKPAEPRGSGVGSSKPSMEGRASYDSLPRVSQELKDALSNLQQTFVVSDATRPDCPIIYASAGFYTMTGYSAKEIIGRNCRFLQGAETDQKEVSKIRDAVKAGKSFCGRLLNYRKDGTPFWNLLTVTPIRDDQGKVIKFIGMQVEVSKYTEGLSDKCMRPNEMPVSLIRYDDRQRENAMSSMTEVVQTVKHRKADTPEMMMMETPKLSNADKDGGSRMAVAGSSPLVTPATPSGGGLKSPLWDLKKEESRMSRLMSGRKSLMGFKVGKRSSVGSREPAVVQEAPEVKMTVERTDSWERAEREKDIRQGIDLATTLERIEKNFVITDPRLPDNPIIFASDSFLELTEYTREEILGRNCRFLQGAETDQTTVDKIRDAIREQKEVTVQLINYTKSGKKFWNLFHLQPMWDQKGELQYFIGVQLDGSDHVEPLRNRLSETTEIQSAKLVKATAGNVDDAVRELPDANLRPEDLWAIHSLSVSPKPHKRNNSSWKAIAKIVQTGEKIGLKHFKPVKPLGCGDTGSVHLVELQGSGELFAMKAMDKSVMLNRNKVHRAIIEREIYSLLDHPFLPTLYTSFQTPTHVCLITDFCPGGELFAALDKQPLKIFREESARFYAAEVVIGLEYLHCLGIIYRDLKPENILLQADGHVVLTDFDLSFLTSSKPHVIKHAASLKRRRSKEFLPPSFVADPSTPSNSFVGTEEYIAPEVISGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRRKTFYNILHKDLTFPSSIPVSLAAKQLIHGLLQRDPSSRIGSNTGANDIKEHPFFEDIYWPLIRCMSPPELHVPLKLIGKESQPKLNPEEGVLDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZ19</accession>
    <name>I1IZ19_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100829864</name>
        <name evidence="2" type="ORF">BRADI_5g13930v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580012.1">
        <property type="nucleotide sequence ID" value="XM_003579964.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZ19"/>
    <dbReference type="EnsemblPlants" id="KQJ83247">
        <property type="protein sequence ID" value="KQJ83247"/>
        <property type="gene ID" value="BRADI_5g13930v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829864"/>
    <dbReference type="Gramene" id="KQJ83247">
        <property type="protein sequence ID" value="KQJ83247"/>
        <property type="gene ID" value="BRADI_5g13930v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829864"/>
    <dbReference type="eggNOG" id="ENOG502RZ3E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073969_1_1_1"/>
    <dbReference type="OMA" id="TFIVIID"/>
    <dbReference type="OrthoDB" id="1716650at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IZ19">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003832">
        <property type="entry name" value="DUF212"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31446">
        <property type="entry name" value="ACID PHOSPHATASE/VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31446:SF8">
        <property type="entry name" value="OS04G0486900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02681">
        <property type="entry name" value="DUF212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ83247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="16251" checksum="DCFFB94269FF5E84" modified="2012-06-13" version="1">MAAGVVNYPLVAGLLAFAVAQSTKFFTTWYKEKRWDARQFIASGGMPSSHSATVTALAVSVGIQEGFRSATFATAMIFACVVMHDAFGVRLHAGKQAEVLNQIVYELPLEHPLSETKPLREILGHTVPQVVAGCILGIVTAVIMLLALGSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1J2K3</accession>
    <name>I1J2K3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100831999</name>
        <name evidence="18" type="ORF">BRADI_5g23920v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the peptidase C19 family. UBP8 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579384.1">
        <property type="nucleotide sequence ID" value="XM_003579336.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J2K3"/>
    <dbReference type="FunCoup" id="I1J2K3">
        <property type="interactions" value="2252"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J2K3"/>
    <dbReference type="MEROPS" id="C19.A11"/>
    <dbReference type="EnsemblPlants" id="KQJ84954">
        <property type="protein sequence ID" value="KQJ84954"/>
        <property type="gene ID" value="BRADI_5g23920v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831999"/>
    <dbReference type="Gramene" id="KQJ84954">
        <property type="protein sequence ID" value="KQJ84954"/>
        <property type="gene ID" value="BRADI_5g23920v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831999"/>
    <dbReference type="eggNOG" id="KOG1867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008279_11_0_1"/>
    <dbReference type="OMA" id="NVSCNCI"/>
    <dbReference type="OrthoDB" id="47475at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070461">
        <property type="term" value="C:SAGA-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000089">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF33">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="17">
        <location>
            <begin position="8"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="16">
        <location>
            <begin position="185"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQJ84954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="563" mass="62682" checksum="2C5443635346EF8B" modified="2012-06-13" version="1">MSTPSAPPPCPHLAAHRLTSRPLRFLRRCLRVRPLGRPEIRRDAREVPRCSPCASSTSPSPSPPRLYACLSCAAVFCASHAASHAATPPSPGHQIAVDVDRAELFCAACGDQVYDSDFDHAVVLAQSSTLCPASTSSASAPSPRKRRRVDYRAWAPDPAESALMSSADPATSARAAAAAAPPGLRGLNNLGNTCFMNSVLQALLHAPPLRNYFLGDRHNRFLCPRRTPMRHRAAGADAKAACLACDLDEIYSATFSGERTPYSPAKFLYSWWQHASNLASYEQQDAHEFFISILDHIHENIKDEQHKSHEQGHGDCCIAHRVFSGILRSDVICTNCGFSSTTFEPCVDISLDLDAGSNNSLANPKLHARNGERNLAGTNSKVSTLMRCLERFTRAERLDAEQKFFCERCKERQESLKQMSIRRLPLVSCFHIKRFEHSSVKKMSRKVDHSLQFPFSLDMAPYLSSSVLRSRYGNRIFPSEASDSDAVSELSSEFEIFAVITHSGKLDAGHYVTYLRLNNLWYRCDDAWVTRVEEHTVRTSQAYMLFYVQKTLYYKACERAGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E3U0</accession>
    <name>A0A0Q3E3U0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g09165v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E3U0"/>
    <dbReference type="FunCoup" id="A0A0Q3E3U0">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ82479">
        <property type="protein sequence ID" value="KQJ82479"/>
        <property type="gene ID" value="BRADI_5g09165v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82479">
        <property type="protein sequence ID" value="KQJ82479"/>
        <property type="gene ID" value="BRADI_5g09165v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E3U0"/>
    <dbReference type="OrthoDB" id="696926at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053253">
        <property type="entry name" value="Sex_diff_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087:SF49">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087">
        <property type="entry name" value="OS07G0539200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="644"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ82479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="741" mass="79560" checksum="E1BA1FA67D19348B" modified="2016-01-20" version="1">MCSGSHAPRRASPPPGPAGSSHAAAGVARSLVRSSRSSPSPFRSAAAALSSSTLHAPSRSYADAVALGGPCDAPALLGKAHSAPSSPRSVWRSEARPRLSSVVVGSLPSLPAAEKGNPWISVPSRSRRPSPLNGRLPKLPGSSRRPNGASSPSPARAAFLRRFRGRCFRCLSTNHRRAACRDPPRCIECWAWGHLASESSRCRAARRSPATPAPLTAARPSLPVRFPAPPPSAEMLSRAITMPAPRRSGSTHSVVMASRFVEHQVFVLRNHGVLAKAIDKHHSASPILVGKAIRGTRHHPEAFFIHFDFPAHRDRPWRELDHGSIERHDLHVRVAIEKMPLHLWSIEGAEQVFGKDVIVDRLYSRTYAKEDTRLFSCWVWCRSLDRIPSDHAFTVFRSGAGRIAPPPEGIRFNTIIHIDLVEDWTVRELYSWFEGLYDGDVPTGDRRRIPVSCRDAFTLAPRRDDTGDDDDLQRRPGRNSGTARAQPPRVDAAPSGLVARVTGARLVDPLAPAPLPVHALAPAPPASLVTASSQRSTSSEDPLAELIASERLSDIDLSTQEFDPMVAATPLLFSPSPAGGRPGLGSFSPLPLGPVSPSLTLPVGESMPQDVATDSDDTDGILQSLFGPAACLGPRRFIAPVLADRQGEARPTPRRSARQAGRASSTPVAQRATICLAKELAVIEPGDQRTDAAASALVQRFKEPLSDVDIDGFAVLTRIDLDALLRAAAQASASRAATQAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EPP9</accession>
    <name>A0A0Q3EPP9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g16660v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88268.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EPP9"/>
    <dbReference type="STRING" id="15368.A0A0Q3EPP9"/>
    <dbReference type="EnsemblPlants" id="KQJ88268">
        <property type="protein sequence ID" value="KQJ88268"/>
        <property type="gene ID" value="BRADI_4g16660v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88268">
        <property type="protein sequence ID" value="KQJ88268"/>
        <property type="gene ID" value="BRADI_4g16660v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EPP9"/>
    <dbReference type="OrthoDB" id="651046at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF15">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="15"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ88268.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="186" mass="21060" checksum="F571145C810805FC" modified="2018-03-28" version="2">MAEAGGKKFSRSYVQWTPKMDSALLDVFVEHHNNGDHAQNGWKPHVYHNAIKAVREKCGVDVTKEKIVSRCKTFDKDYEILSKILAQSGFGWDWENKVLLMDSDEVWDKYVEANEKAAIYKNKVIHNWHEICIVYSKDKANGEGAMTGNETEVDATAQENDMSPGIVEPAPKRQRASEALLCMSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ESJ6</accession>
    <name>A0A0Q3ESJ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100825034</name>
        <name evidence="11" type="ORF">BRADI_4g31770v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238230.2">
        <property type="nucleotide sequence ID" value="XM_010239928.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ESJ6"/>
    <dbReference type="FunCoup" id="A0A0Q3ESJ6">
        <property type="interactions" value="863"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3ESJ6"/>
    <dbReference type="EnsemblPlants" id="KQJ90470">
        <property type="protein sequence ID" value="KQJ90470"/>
        <property type="gene ID" value="BRADI_4g31770v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825034"/>
    <dbReference type="Gramene" id="KQJ90470">
        <property type="protein sequence ID" value="KQJ90470"/>
        <property type="gene ID" value="BRADI_4g31770v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825034"/>
    <dbReference type="OrthoDB" id="6354171at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3ESJ6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000554">
        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055185">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055186">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031140">
        <property type="entry name" value="IDD1-16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593:SF168">
        <property type="entry name" value="PROTEIN SHOOT GRAVITROPISM 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22992">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22996">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="472"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="350"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ90470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="502" mass="53406" checksum="970C877CBA067DA9" modified="2016-01-20" version="1">MLGPTAGAGPPPEEDMPEPFGSLQIATTSAGAATKRKRRPAGTPDPDAEVVSLSPRTLLESDRYVCEICNQGFQRDQNLQMHRRRHKVPWKLLKREAGEAARKRVFVCPEPSCLHHDPSHALGDLVGIKKHFRRKHSGHRQWACARCSKAYAVHSDYKAHLKTCGTRGHSCDCGRVFSRVESFIEHQDTCNAGCPQAAGSDVPAATARGVAAVASSQQQQQVPAPAASLSRTASSTSPSSDIVISPVAWPSAGAGAMRSPTAAAFHRFDQAPSPPEGRGGGHNLELQLMPPSSGCTVGGSLAPGVAPSCYGAPPCSPGDDAAMQLQLTIGFCSGDVNRGELRPFGRDDAAAHARAKQEAAREELRQAMGEKAAADEARAQAKRQGELAEQELASAKRMRHQAQVELSRAHALREHAVRQVNATLLQITCLSCRQKFRAAAATTAIRPAPAAAMSAEQQVACSYVSSSVVTEGGDADTGNVDDQPLNLGGFRRRQQHARMDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F426</accession>
    <name>A0A0Q3F426_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Ile)-lysidine synthetase</fullName>
            <ecNumber evidence="1">6.3.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100842962</name>
        <name evidence="8" type="ORF">BRADI_3g09901v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(34) in tRNA(Ile2) + L-lysine + ATP = lysidine(34) in tRNA(Ile2) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="1"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234122.1">
        <property type="nucleotide sequence ID" value="XM_010235820.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F426"/>
    <dbReference type="STRING" id="15368.A0A0Q3F426"/>
    <dbReference type="EnsemblPlants" id="KQJ94327">
        <property type="protein sequence ID" value="KQJ94327"/>
        <property type="gene ID" value="BRADI_3g09901v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842962"/>
    <dbReference type="Gramene" id="KQJ94327">
        <property type="protein sequence ID" value="KQJ94327"/>
        <property type="gene ID" value="BRADI_3g09901v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842962"/>
    <dbReference type="OrthoDB" id="198857at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3F426">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032267">
        <property type="term" value="F:tRNA(Ile)-lysidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010098">
        <property type="term" value="P:suspensor development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="TRNA(ILE)-LYSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033:SF5">
        <property type="entry name" value="TRNA(ILE)-LYSIDINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="7">
        <location>
            <begin position="58"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ94327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="672" mass="74927" checksum="DF64EC11F63ABE4F" modified="2016-01-20" version="1">MLLLLSCRPSPSPCLPLRRLLRCCCTSSSVSAPSPIAPYHQSFARRMALAGIHPEHRIAVGVSGGPDSMALCVLVAAWKKAVDGEPGRKSGEDGSVGPGFVDGLLGVVVDHGLRPESAEEAQVVRDRVHGMGVLCEIAKCEWPNGRPKLGHLQEAAREIRYQKLLDICIKQRIGVLLIAHHSDDQAELFVLRLSRNSGVLGLAGTAFVSQLFAPNLKYDGHNFSRYGILLVRPMLEFSKDDMYKICQGSNNLWVEDPTNTSMQYARNRIRASLKCLSAEGTFLSELQRLIYACQLTRAHVDNTCSLVVKKSVTITEYGYIVIDLEKLDPQNVDDLCLSRYLACVLQFVSQRHRPIRGRSAQLFMDYIRNTPCKGALTVAGCYLCAFPGSKGAKILVCCSVDLMESFSAEMSYKCSYEEQPPPVPEIEQIVLEAHSYSNQFLQCTNIPFVNYRSSSDVLDRAKDLNIIGDSTLEKLTYLQTEELQKFTTTKHRHEDQYLEKTSFRDSKVLCLWPGETCHFLSRFLITWNALKVLANGICSHDSKKSLCQHCVVNEDGSLTVRRMHDTDWLLLAEVSKIHSVEQNQNVSKVLSKKLDDDELLNHYRYLQLSATKAIEILRSIPASARRTLPVLTNSQGDILSIPSIGFRCCPSLSIKAVFHPRVPLGGGYVSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FGE0</accession>
    <name>A0A0Q3FGE0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100839716</name>
        <name evidence="5" type="ORF">BRADI_3g38527v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757040.1">
        <property type="nucleotide sequence ID" value="XM_014901554.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FGE0"/>
    <dbReference type="STRING" id="15368.A0A0Q3FGE0"/>
    <dbReference type="EnsemblPlants" id="KQJ98703">
        <property type="protein sequence ID" value="KQJ98703"/>
        <property type="gene ID" value="BRADI_3g38527v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839716"/>
    <dbReference type="Gramene" id="KQJ98703">
        <property type="protein sequence ID" value="KQJ98703"/>
        <property type="gene ID" value="BRADI_3g38527v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839716"/>
    <dbReference type="OrthoDB" id="272703at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FGE0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000549">
        <property type="entry name" value="Plastid-specific 30S ribosomal protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF17">
        <property type="entry name" value="28 KDA RIBONUCLEOPROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="86"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="179"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ98703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="283" mass="30718" checksum="008B7F0E82BBDA74" modified="2016-01-20" version="1">MALPLVRLLLPSLPACQPPLRHPEHSSPPPRHGHVSFSVSAAARSSSCSRAAGFAACAASASVAPAPVPAEAETEAAEEEPVGPRTRLIAMNIPWDYTPDDMRALFEKQGSVVSVELSMYSSKKNRGLAFVTMGSEEEALSAINNLNSSTLNDRTIKVDFAKPRKKQPTVPSAPMQNKHIVFVGNLTWRVRSRHLRELFASTPGVQSVEVIFQTTTPRRSAGYAFVSFSSKEEAEAAISTFNGKELLGRPINVTFKEETAKKKISSHSEEENLEAESSEQSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FTV5</accession>
    <name>A0A0Q3FTV5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100835845</name>
        <name evidence="19" type="ORF">BRADI_2g03947v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">Bd21</strain>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754541.1">
        <property type="nucleotide sequence ID" value="XM_014899055.2"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FTV5"/>
    <dbReference type="EnsemblPlants" id="KQK02824">
        <property type="protein sequence ID" value="KQK02824"/>
        <property type="gene ID" value="BRADI_2g03947v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835845"/>
    <dbReference type="Gramene" id="KQK02824">
        <property type="protein sequence ID" value="KQK02824"/>
        <property type="gene ID" value="BRADI_2g03947v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FTV5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
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        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="598"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="629"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="832"/>
            <end position="976"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="67"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="455"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="979"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1019"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1309"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="598"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="85"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="272"/>
            <end position="335"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="346"/>
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        </location>
    </feature>
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        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
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        <location>
            <begin position="1019"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
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            <end position="1047"/>
        </location>
    </feature>
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            <end position="1216"/>
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            <end position="1251"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FU48</accession>
    <name>A0A0Q3FU48_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gag1-like clamp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100834614</name>
        <name evidence="2" type="ORF">BRADI_2g04471v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02928.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK02928"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="190" mass="20993" checksum="718342A6C5FFD02D" modified="2018-03-28" version="2">MAARGGGIGGGGSAGRVGDWDVNLGARWDWRAIPQLLSSACIFICSGGCFGCCEKAVKHVGELSKSLLTQDLHPTVGEEFWSTTTIEVDQADLRGIPPFHPTSFPLDQHGAGSSHNPSEFGFSLWEQIREEWTDNRKEQPVVKQIHEPVLSWNAAYESLLGSNKPFPQPIPLHEMVDFLVDMWEQEGLYD</sequence>
</entry>
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            <strain evidence="4 5">Bd21</strain>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
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    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
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        <property type="gene ID" value="BRADI_2g02830v3"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
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        <lineage>
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    <reference evidence="4 5" key="1">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="4">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="5">cv. Bd21</strain>
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    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
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            <strain evidence="3 4">Bd21</strain>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
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        <property type="nucleotide sequence ID" value="XM_003564352.4"/>
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        <property type="gene ID" value="BRADI_2g52417v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835510"/>
    <dbReference type="Gramene" id="KQK10168">
        <property type="protein sequence ID" value="KQK10168"/>
        <property type="gene ID" value="BRADI_2g52417v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835510"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GGG2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025315">
        <property type="entry name" value="DUF4220"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007658">
        <property type="entry name" value="DUF594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31325">
        <property type="entry name" value="OS01G0798800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13968">
        <property type="entry name" value="DUF4220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04578">
        <property type="entry name" value="DUF594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4220" evidence="2">
        <location>
            <begin position="60"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK10168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="695" mass="79009" checksum="515A1C30D3F56070" modified="2016-01-20" version="1">MKGNGTSTHNMSMREGLLTVWNEWEIRVLVLTSLALQVFLLFSAGIRKRNVSGLLGVLLWLAYLLADSIAIYALGYLSQARVPKGVDDPEFFNRAHGIQVFWAPFLLLHLGGQDTITAFSIEDNELWKRHLLSLLSQVALAVYVFSKSRPGDDILYPAVFMFLSGILKYGERTWALKCASMDNLRSGMVTTPDPGPNYAKFMEEYRFTREAGLQAEIVIEPERRAGVTSAAITEETVPYATVITEARCFFVTFKRLFVNLILSFHDRTQSQATFLRLTPGQAYKVIEIELSLMYDTLHSKAVVIHTWYGRLFRCVTLVSTTTACVLFNVLRKGRRGSSYDGTDIFITNMLFGGALFLEVSAIGMMLVSYWTYAALQGSNCHWLSHLILRCIKYFRPENRAKWSNLMAQHNLISFCLQDKPTLLTKVLSLLGLKGRWEGWLNIRHIDVSSDLKTLVFRELKDKAASIVDAESYRKFSNHRGQWALQCKGYYKELGWSVEVEFDESILLWHIATDLCFYSDDSNDNTKLAQYVSISRAVSDYMLFLLAERPFMLTAGIGQIRFGDTCAEAKNFFGREMARTDKRAAARMVLEVNAEIAPKDVKGDRSKSVLFDACRLAKSLLELQPANKRWRLIRVVWVEMLCYAASKCQSNFHAKQLSNGGELLTVVWFLMTHLGMGEQYRIEAGHARAKLIVEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GMW8</accession>
    <name>A0A0Q3GMW8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_1g03360v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12383.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GMW8"/>
    <dbReference type="FunCoup" id="A0A0Q3GMW8">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GMW8"/>
    <dbReference type="EnsemblPlants" id="KQK12383">
        <property type="protein sequence ID" value="KQK12383"/>
        <property type="gene ID" value="BRADI_1g03360v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12383">
        <property type="protein sequence ID" value="KQK12383"/>
        <property type="gene ID" value="BRADI_1g03360v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GMW8"/>
    <dbReference type="OrthoDB" id="676172at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000444">
        <property type="entry name" value="probable L-type lectin-domain containing receptor kinase S.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033724909" description="Protein kinase domain-containing protein" evidence="8">
        <location>
            <begin position="28"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="322"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="623"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="647"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK12383.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="715" mass="78508" checksum="C5FDD88B86B67BD0" modified="2018-03-28" version="2">MAIQLKHTTHCLCSILLLVFLMSQAKAEPCKCPDSDQQDRWADMGDLGLQYHLIDQDSGLVRTVGSNLLRDLSDSDAKNKNHTWVLSTIDGSVALLNHRNSPGAEAASFVITIVYQPLWNQNQSGITFLILPAEALEDLEAHQHGPRSSDGIMAVDISFASSSDENYLSMLYTVCIKYNHFERRLSVYMGVAEDPQPDNAIAEVHLNISTVVSQNVYFGLLSTVAQKLHMGAGGFRATILYYIPRAYDNNRGSLSRKVTILSSVLGSAAATAALAAAIAYYLNSKYRQRRKELEKLARSMERLPGVPIQVEFADIKKATRNFHDDMKLGAGGFGAVYRCTLPAAASKTGQPMEVAVKRFTRRGEQNRCYDDFLAEVSIINRLRHKNIVPLVGWSYHKGEPLLVFEFMTNGSLDQHLFPRAAGSANAAIRRWATRYEIVRGIATGLHYVHHEYEPVVLHRDIKASNIMLDSSFCARLGDFGLACTVAVDRNSATGVGGTWGYIAPEYAYCGKATRQTDIYALGVLILEVVTGKRALLAADLVVDEDDVHITDRVWRLHREGRLGECVDTVLLAASSEDNDSDEQLAGLGEDTERLLLLGLACSNPNPSDRPSMPDVVQVIAKSAPPPEVPSQKPRFVWPPPEWDANTGDDSTSTAMATTNPDSRSRVSMDELQTLGMPPTAPAPAAVGHASFELEHSVYATATSSLLPGEIPRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GRK0</accession>
    <name>A0A0Q3GRK0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC104581907</name>
        <name evidence="2" type="ORF">BRADI_1g07611v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13037.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313167.1">
        <property type="nucleotide sequence ID" value="XM_024457399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GRK0"/>
    <dbReference type="EnsemblPlants" id="KQK13037">
        <property type="protein sequence ID" value="KQK13037"/>
        <property type="gene ID" value="BRADI_1g07611v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581907"/>
    <dbReference type="Gramene" id="KQK13037">
        <property type="protein sequence ID" value="KQK13037"/>
        <property type="gene ID" value="BRADI_1g07611v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104581907"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK13037.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="102" mass="11971" checksum="8EBA6612FFF5A7FB" modified="2018-03-28" version="2">MLQVQEVLRLFKIRWKLKKEELRLDQSYIVLSIPTRKESLWMPILQERWLIRKRSSNNGDSQTGQKKPTKRRRTSNPTTRKSSSKKDSQANASMQNALVAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GWK0</accession>
    <name>A0A0Q3GWK0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100829440</name>
        <name evidence="6" type="ORF">BRADI_1g22460v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK15391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560003.3">
        <property type="nucleotide sequence ID" value="XM_003559955.3"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A0Q3GWK0">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GWK0"/>
    <dbReference type="EnsemblPlants" id="KQK15391">
        <property type="protein sequence ID" value="KQK15391"/>
        <property type="gene ID" value="BRADI_1g22460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829440"/>
    <dbReference type="Gramene" id="KQK15391">
        <property type="protein sequence ID" value="KQK15391"/>
        <property type="gene ID" value="BRADI_1g22460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829440"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="SGNH_plant_lipase_like"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
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        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF599">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
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    </dbReference>
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        <property type="entry name" value="SGNH hydrolase"/>
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    </dbReference>
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    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129176" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="31"/>
            <end position="378"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK15391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="40414" checksum="4057BCA73F35AED2" modified="2016-01-20" version="1">MSTVPFPSRLCGGLLVVMVALLLPVHSAAGKSSYTGVLSFGDSLADTGNALAHTGGGVGSQLPYGETFFGHPTGRASDGRIVLDFIVEELGMEYPTPYFAGKTAADFQHGVNFAYGGATALDPEFLRSRGLTPFVLLSLANQTAWFRQVLHLVRSVHAQRELMARSLVMVGEMGINDYLVAFFAKRTPSEVEPLVPHVIQAVRSLVNEVISAGAKTVVVRGMIPLGCQPQMLALFENTAGAEYNGKTGCLTRLNELARIHNRKLFRMVLELRLANLGRGVDIFYADQYGPVDSIVRTPRRYGFGEKPLVACCGGGGGKYNFGFSTFCGVEGATLCSDPSKYVSWDGIHMTDTANGRVAAAVLRSTGILRPKSLGVASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GWM7</accession>
    <name>A0A0Q3GWM7_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">BRADI_1g19142v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GWM7"/>
    <dbReference type="EnsemblPlants" id="KQK14869">
        <property type="protein sequence ID" value="KQK14869"/>
        <property type="gene ID" value="BRADI_1g19142v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14869">
        <property type="protein sequence ID" value="KQK14869"/>
        <property type="gene ID" value="BRADI_1g19142v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GWM7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK14869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="30" mass="3542" checksum="B7DFEF0A032666E7" modified="2016-01-20" version="1">MTSCKDVPDCNFIHAAIQCRKYEHMLIQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IBE2</accession>
    <name>A0A0Q3IBE2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g14900v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3IBE2"/>
    <dbReference type="EnsemblPlants" id="KQJ83432">
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        <property type="gene ID" value="BRADI_5g14900v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83432">
        <property type="protein sequence ID" value="KQJ83432"/>
        <property type="gene ID" value="BRADI_5g14900v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IBE2"/>
    <dbReference type="OrthoDB" id="10252102at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="Sedlin"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12696" checksum="7804903B9AA74D2D" modified="2016-01-20" version="1">MATTACFVIVSKNDIPIYEAEVGSAPKKEDLAYHHQFILHAALDVVQDLAWTTSAIFMLLHDSRSEDGIKGFFQEVHELYIKIFLNPLYLPGSRIASSHFDTKVRALARKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IGK4</accession>
    <name>A0A0Q3IGK4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g17646v3</name>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IGK4"/>
    <dbReference type="EnsemblPlants" id="KQK05051">
        <property type="protein sequence ID" value="KQK05051"/>
        <property type="gene ID" value="BRADI_2g17646v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05051">
        <property type="protein sequence ID" value="KQK05051"/>
        <property type="gene ID" value="BRADI_2g17646v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IGK4"/>
    <dbReference type="OrthoDB" id="587756at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF121">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="97"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK05051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="362" mass="40094" checksum="BBBA499AFC11C0FB" modified="2016-01-20" version="1">MAAPRPWKDLLRAALRAIIRHVPCEADRLGLGAVCFSWRLALARLAPLPPAPRQLPWVLCPSPLGPTFSCLLCGGGAAHPPPSTATRPRRERAASARHEILNLRSGKRLPIPDDFPMRLGKRAPMVICAATLSHPPDNPRCVAAAIVEMLPVGTDSPLLTFWRMGHPVGAKVTRSGNEREDIIHREGAFLVLSRAETLLAYTPRFHGFKGDLLIGCDVVPFLRGGRDYGDHRVHGRYLVESRGELLMVGRLAPRPLAPTSAFRVFQMERTRALGSGNVGYTWRELPALDGRMLFVQRVCSRSYEVSDYPGFEDGVYFLDDGVDVCDPCFDNGKWSAPPSHVDRRFPPGQVLSVYRSPVWFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IZW1</accession>
    <name>A0A0Q3IZW1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100846080</name>
        <name evidence="3" type="ORF">BRADI_2g58912v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564939.1">
        <property type="nucleotide sequence ID" value="XM_003564891.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IZW1"/>
    <dbReference type="STRING" id="15368.A0A0Q3IZW1"/>
    <dbReference type="EnsemblPlants" id="KQK11235">
        <property type="protein sequence ID" value="KQK11235"/>
        <property type="gene ID" value="BRADI_2g58912v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846080"/>
    <dbReference type="Gramene" id="KQK11235">
        <property type="protein sequence ID" value="KQK11235"/>
        <property type="gene ID" value="BRADI_2g58912v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846080"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051340">
        <property type="entry name" value="Haloalkane_dehalogenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42977:SF3">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42977">
        <property type="entry name" value="HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="124"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK11235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="378" mass="42361" checksum="B7764BAF6C75A675" modified="2016-01-20" version="1">MAPPLAAPPFHAALPRRLLLLRRQRPLPSWRRAARPDDQDYLYVDDSIGDGFSFSGGKYAEAEELSKSDEWFAQGRMVKAHALYGSKEKAKDPFFGLTMGKESQSSDDVFSWFCVEAGSSSNHTVLLVHGFPSQAYSYRNVLPVLSDKYHPIAFDWLGFGFSDKPQPKYGFDYTLDEFTSSLESLINAVAPDKVSIVVQGYFAPIVVKYASKHQDKLNHLILVNPPITDKHAKLPSTLACFSNFLLGEVFSQDPLRASDKALTSSGPYMMKEEDATVYRRPYLVSGSSGFALNAMTRAMGKDLKVYIESMRSILVSDSWNTKTTICWGLRDRWLSYDGVEDFCDGVKHKFVELPMAGHHVQEDRGEELGNIIKRILSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JHL1</accession>
    <name>A0A0Q3JHL1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100840933</name>
        <name evidence="12" type="ORF">BRADI_3g32470v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RING-type zinc finger family. NIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572033.2">
        <property type="nucleotide sequence ID" value="XM_003571985.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JHL1"/>
    <dbReference type="EnsemblPlants" id="KQJ97655">
        <property type="protein sequence ID" value="KQJ97655"/>
        <property type="gene ID" value="BRADI_3g32470v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ97656">
        <property type="protein sequence ID" value="KQJ97656"/>
        <property type="gene ID" value="BRADI_3g32470v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ97657">
        <property type="protein sequence ID" value="KQJ97657"/>
        <property type="gene ID" value="BRADI_3g32470v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840933"/>
    <dbReference type="Gramene" id="KQJ97655">
        <property type="protein sequence ID" value="KQJ97655"/>
        <property type="gene ID" value="BRADI_3g32470v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97656">
        <property type="protein sequence ID" value="KQJ97656"/>
        <property type="gene ID" value="BRADI_3g32470v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97657">
        <property type="protein sequence ID" value="KQJ97657"/>
        <property type="gene ID" value="BRADI_3g32470v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840933"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JHL1">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000505">
        <property type="entry name" value="NEP1-interacting protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46151:SF7">
        <property type="entry name" value="NEP1-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46151">
        <property type="entry name" value="NEP1-INTERACTING PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="190"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ97656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="235" mass="24173" checksum="03B5DD3FCB6BD2BF" modified="2016-01-20" version="1">MDTWAMASPSSAGPSPPAPEPEPERGQWGGCGSVACRVACAVATCVLAAVGSAAGAVAGAAIGLATESGVVRGAGVGAISGAVFSIEVAESSRQLWRATGSPAWTVLYVVDIIFSLLSGRLVREKVGPAVQNAVQSQISAINAPFAEQSDLFETGSGARGGLPASALRRLPEIRIDEDTAVDAGGEALCCSVCLQDLQVGEPARRLPVCRHVFHAPCIDRWLARHASCPLCRRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K0K8</accession>
    <name>A0A0Q3K0K8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100833145</name>
        <name evidence="7" type="ORF">BRADI_1g72910v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229109.1">
        <property type="nucleotide sequence ID" value="XM_010230807.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K0K8"/>
    <dbReference type="EnsemblPlants" id="KQK23355">
        <property type="protein sequence ID" value="KQK23355"/>
        <property type="gene ID" value="BRADI_1g72910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833145"/>
    <dbReference type="Gramene" id="KQK23355">
        <property type="protein sequence ID" value="KQK23355"/>
        <property type="gene ID" value="BRADI_1g72910v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833145"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K0K8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17351">
        <property type="entry name" value="MFS_NPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="501"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="546"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK23355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="571" mass="61446" checksum="2D087372FE477EC6" modified="2016-01-20" version="1">MEASDDERPLLIHYQPPQDAGSQCTCDGTVDIDDQPATKKSTGNWRACFFILGAEVSEGICFFGVQKNLVTFLTSVLHESNVDAARSVSTWTGTCFFTPLIGAFLADTYLGRYLTVVTFLSVYVVGLLVMTFSASLPLLMPSSYSIDIQHVVVYLGLYLVALGTGGIKPCASSFGADQFDSADPVEQVAKGSFFNWYYFLINIGSVLSATLLVWVQDNIGWGVGFGILMVLMVFGLTVFVTGKKIYRYKKLGASPLKRVSQVVVAALRNYRLKLPDDISALHGVPSLAGANCKTEHTNQFRFFDKAAIVLPPLDGKGSSVMSPWRLCTVSQVEELKTLLRLCPVWASLLFFFAVTGQMTSTLIEQGMAMDNRVGGFTVPPASLSTFDIITVVALIPLYDVFLVPLARRATGKNRGISQLQRIGTGLALSAAAMAYSALVETRRLAAAAGADQSAAAVAAPLSIMWQAPSYIVLGAAEVFASIGALEFFYDESPESMKSLGAALAQLAIAGGSYLNSALLGVVASATARGGAPGWIPDDLNQGHLDYFFWMMAGLSVLNLLHFMYCSMRYKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KD39</accession>
    <name>A0A0Q3KD39_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g66571v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KD39"/>
    <dbReference type="EnsemblPlants" id="KQK22332">
        <property type="protein sequence ID" value="KQK22332"/>
        <property type="gene ID" value="BRADI_1g66571v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22332">
        <property type="protein sequence ID" value="KQK22332"/>
        <property type="gene ID" value="BRADI_1g66571v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3KD39"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="74" mass="8318" checksum="F1EBDADD4F48A120" modified="2016-01-20" version="1">MNLVLSFAGRWFVSQISVTGVFWLRPATFRLLRQQRFLGSTWFGDLETASNFAHGTPPASAGGRQRRLSTRTCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KGA3</accession>
    <name>A0A0Q3KGA3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g52011v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KGA3"/>
    <dbReference type="STRING" id="15368.A0A0Q3KGA3"/>
    <dbReference type="EnsemblPlants" id="KQK10105">
        <property type="protein sequence ID" value="KQK10105"/>
        <property type="gene ID" value="BRADI_2g52011v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10105">
        <property type="protein sequence ID" value="KQK10105"/>
        <property type="gene ID" value="BRADI_2g52011v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3KGA3"/>
    <dbReference type="OrthoDB" id="682522at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45085">
        <property type="entry name" value="F21J9.14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45085:SF3">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="2">
        <location>
            <begin position="85"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK10105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="178" mass="18671" checksum="2EBC45526368AFF2" modified="2016-01-20" version="1">MDWHVRRLLNRVSIALAAVATAALRHLFRHHSSSSAPPASPPARPPTPPVLPALATPQRSPLHVGARPARLAARLRLLGGSSRVLCLAAGAGQAVDALHVAGVGDVTGVDLVDFPPLVRRADPHNLPFFDDAFDLVLSDDPAALTGALFPSRFAAAVERTVRPGGAIALAVDRRLIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LAM8</accession>
    <name>A0A0Q3LAM8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100833425</name>
        <name evidence="8" type="ORF">BRADI_4g26400v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium sensor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calmodulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237972.1">
        <property type="nucleotide sequence ID" value="XM_010239670.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LAM8"/>
    <dbReference type="STRING" id="15368.A0A0Q3LAM8"/>
    <dbReference type="EnsemblPlants" id="KQJ89557">
        <property type="protein sequence ID" value="KQJ89557"/>
        <property type="gene ID" value="BRADI_4g26400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833425"/>
    <dbReference type="Gramene" id="KQJ89557">
        <property type="protein sequence ID" value="KQJ89557"/>
        <property type="gene ID" value="BRADI_4g26400v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="429467at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LAM8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000034">
        <property type="entry name" value="Calmodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000251">
        <property type="entry name" value="Calmodulin-related protein 97A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF54">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="78"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="114"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="210"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="210"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ89557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="255" mass="28646" checksum="4DA272E45CE21DE9" modified="2016-01-20" version="1">MDELSQEQLDEFRAAFSLFDRDGDGTITLEELGTVMRSLGQRPTEAELRDMIAEVDADGNGAVDFAEFLALVDRKLLDAEDDLRDAFRVFDADGNGFISLDELRRVMLDLGERLSDEELAQMLLEADGDGDGQINYSEFAKLMMSKLELAGSKPMVPPLFKNGIRVCRYLLAFRILQVGRNVVRRRYRSGPMDWMSGPILKVMSGPFEKVLTRPTEKERSGPLEEEKEKEKSGPLEEKSAGGDHRRRCGTSCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LQ86</accession>
    <name>A0A0Q3LQ86_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g12843v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LQ86"/>
    <dbReference type="EnsemblPlants" id="KQJ94725">
        <property type="protein sequence ID" value="KQJ94725"/>
        <property type="gene ID" value="BRADI_3g12843v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ94725">
        <property type="protein sequence ID" value="KQJ94725"/>
        <property type="gene ID" value="BRADI_3g12843v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LQ86"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ94725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="91" mass="10395" checksum="1BB041C8EF7174B5" modified="2016-01-20" version="1">MPLERVWANPCVALPIIPITHFTHTHWTSGQYWANRKIISQTQADGSPESKSSNHPRKKNPITIPGFVEAMHHKHKYLQLYSQSIARVAPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3MY08</accession>
    <name>A0A0Q3MY08_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">E2F/DP family winged-helix DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100837384</name>
        <name evidence="13" type="ORF">BRADI_2g46527v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754350.1">
        <property type="nucleotide sequence ID" value="XM_014898864.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3MY08"/>
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            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="259" mass="29254" checksum="12ED2C749C61D33C" modified="2016-01-20" version="1">MAPPRGDTAAAASASLDLTGVHILEASMPPPLPECGGNAAERKEEGDKNRKKEKAETQRITGWGLREYSKIVCEKVEAKGRTTYNEVADEVYSELKSMAHIGQGFEEKNVRRRVYDAFNVLIALRVIAKEKKEIKWMGLSNYRYETIKKLEEVRKELNNRIKNKKKLLKEIGKQFDDLQNIKLRNQTLQSSAENANGIQLPFLLVKTSRKASVEIEISEDSKFARFEVTGAPFTLHDDLSILEGMRHNSIARADLGTLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3N093</accession>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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        </lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
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    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK09870.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="116" mass="12572" checksum="75B854B47BD1747E" modified="2018-03-28" version="2">MRAEQPCRQLGTPWNLASVSATAARSGGILASFVQVLRRAEDKRESFDRQLPGAEPACCPLSSSFSFLFSGFANTSNIDNRSRSGVVGIPRSTCWVWLACVDCGACGRNLIRDGMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3N6M9</accession>
    <name>A0A0Q3N6M9_BRADI</name>
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        <recommendedName>
            <fullName evidence="4">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g03080v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK12342.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3N6M9"/>
    <dbReference type="EnsemblPlants" id="KQK12342">
        <property type="protein sequence ID" value="KQK12342"/>
        <property type="gene ID" value="BRADI_1g03080v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12342">
        <property type="protein sequence ID" value="KQK12342"/>
        <property type="gene ID" value="BRADI_1g03080v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3N6M9"/>
    <dbReference type="OrthoDB" id="654755at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009827">
        <property type="term" value="P:plant-type cell wall modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051955">
        <property type="entry name" value="PME_Inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080:SF140">
        <property type="entry name" value="PECTINESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080">
        <property type="entry name" value="PECTINESTERASE INHIBITOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033725099" description="Pectinesterase inhibitor domain-containing protein" evidence="3">
        <location>
            <begin position="30"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="4">
        <location>
            <begin position="34"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK12342.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="232" mass="23670" checksum="1BB0C1C9FCA24A07" modified="2018-03-28" version="1">MAACRRSRPTASLLLMSLLLSSPIVLVSGGLPAKEACLKSPDPAFCAKFLASIPDSKSAPDARGLAELAIRAAAKAGAEMGSSARAKLDAVTVKGPEWLCMDACVADVEEAVSRLDVDKGKKSSKSKAMGDAKFEDARDYVETAESDGLTFNCDLCRGGLPAPVQTGLLPKDNEFQKIMGVLGALLKLVPGGGDGPSPAPAPGPEPELGPSSGPAPAPAPSPHPFSLLGQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NFQ5</accession>
    <name>A0A0Q3NFQ5_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g27516v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16222.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NFQ5"/>
    <dbReference type="EnsemblPlants" id="KQK16222">
        <property type="protein sequence ID" value="KQK16222"/>
        <property type="gene ID" value="BRADI_1g27516v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16222">
        <property type="protein sequence ID" value="KQK16222"/>
        <property type="gene ID" value="BRADI_1g27516v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NFQ5"/>
    <dbReference type="OrthoDB" id="696112at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK16222.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="301" mass="34385" checksum="A8114BBA1E49D585" modified="2018-03-28" version="2" fragment="single">MWTLIFQCKIPGRTEDDFNTARVDKADVTLSTIISFKEQLGFTGRDYVYYKKRSGLDVASLQVIDLCKHALQMVEDLAAEKEVRLVLSKEEQQQKNVNITSVKRPLEKEPEEDDDDDVGSYDALDAYKDWLKENMEESLSPAYENSISKYNDIQAEGDSQETIESRGSSTRPPNQWPSHARYNKDKKAFKKRGRGTLKGLIATRKRLMQCTHKLKVEFSKNLGGPCGENARTFVDEVVTFTRLHTPLIGIQRLEGCARLVRLTIVRAILDGWDIAKNNETKDMILKIAKERYKGWRSSLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NVE4</accession>
    <name>A0A0Q3NVE4_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g61036v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK21478.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NVE4"/>
    <dbReference type="STRING" id="15368.A0A0Q3NVE4"/>
    <dbReference type="EnsemblPlants" id="KQK21478">
        <property type="protein sequence ID" value="KQK21478"/>
        <property type="gene ID" value="BRADI_1g61036v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21478">
        <property type="protein sequence ID" value="KQK21478"/>
        <property type="gene ID" value="BRADI_1g61036v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NVE4"/>
    <dbReference type="OrthoDB" id="670961at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK21478.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="218" mass="22634" checksum="0CDD7B89EDA9DAA9" modified="2018-03-28" version="2">MKVGWPPYLPLPGPPPVVLGASPGSGDGGGGGGGGSGGQSVDTSARRHRRMSAPSTGIRLMPAFNDAAGDDDGGSWGLSSSSSGGLDLGPDASQPRYNRHRLYPEFSQMSNMGRLLFHGSALGSRTTDDDVLVMDGVLVANSSSSGPRRSSSFSDLGFVHAGYPGCSGRYASNRQCSYGKEESCAVPPMNRRHSEVSSLIPVVFSFEIGHSKQLLGCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PJF5</accession>
    <name>A0A0Q3PJF5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4220 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g26073v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PJF5"/>
    <dbReference type="STRING" id="15368.A0A0Q3PJF5"/>
    <dbReference type="EnsemblPlants" id="KQJ89497">
        <property type="protein sequence ID" value="KQJ89497"/>
        <property type="gene ID" value="BRADI_4g26073v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89497">
        <property type="protein sequence ID" value="KQJ89497"/>
        <property type="gene ID" value="BRADI_4g26073v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3PJF5"/>
    <dbReference type="OrthoDB" id="1689146at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3PJF5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025315">
        <property type="entry name" value="DUF4220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31325">
        <property type="entry name" value="OS01G0798800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13968">
        <property type="entry name" value="DUF4220"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4220" evidence="2">
        <location>
            <begin position="47"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4220" evidence="2">
        <location>
            <begin position="203"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ89497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="447" mass="50701" checksum="FD4A21F52D252E2C" modified="2016-01-20" version="1">MLSLLQVLNEWEIQLLVLLSFSLQVFLFFTGGLRRRSTCSLLRFSMWVAYLGADLVAVYALGFLSRHKDVTAGKGTLKEAHPLAFLWAPFLLIHLGGQDTITAFAIQDNDLWLRHLLKLAVQVVVALYVFWVSTTGRGHRNVQLPGIFVFIAGIVKYGERTLALMYGNLKSMSSSSITIGNENIEDLNRLVLTSDILVDHLPKLLEVELDLMYDDIYTKAMVLRTRSGIIFWCVSQVSLVVGFVLFIVGAVNNKHKYSRVDLAITCVLFTGGLCLEVCAVFIFLMSPWTWAWLKARSRHWLARISSFLLSSKIGRPERRPLWSNSMGQYNFLCYVSSDHQLGLSKLVKKVTKKVMRLVVGAREETKSFLWMSKLLDTEYVEVDKKIMDSVVDMICRDRPGESIMNAQEWPNLGALLAKLLPDFGASFGYGIVCFHIFTEAHLSNYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3Q041</accession>
    <name>A0A0Q3Q041_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100842349</name>
        <name evidence="3" type="ORF">BRADI_3g14690v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571372.1">
        <property type="nucleotide sequence ID" value="XM_003571324.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3Q041"/>
    <dbReference type="STRING" id="15368.A0A0Q3Q041"/>
    <dbReference type="EnsemblPlants" id="KQJ95017">
        <property type="protein sequence ID" value="KQJ95017"/>
        <property type="gene ID" value="BRADI_3g14690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842349"/>
    <dbReference type="Gramene" id="KQJ95017">
        <property type="protein sequence ID" value="KQJ95017"/>
        <property type="gene ID" value="BRADI_3g14690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842349"/>
    <dbReference type="OrthoDB" id="687020at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF466">
        <property type="entry name" value="OS08G0138100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044546093" description="Phytocyanin domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="2">
        <location>
            <begin position="24"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ95017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="195" mass="20425" checksum="EFCAF51A04AB869C" modified="2016-01-20" version="1">MAITKALLLTTIASATLFGMALGASYTVGAPAGSWDLKANYTQWVSGRRFFPGDSLRFQYPAAATHNVLEVTKAAYDTCSNISSIPGSGGSINSSAVIATYQTGNDVILLAASGVTRYFVCGFPGHCAAGMKLKVHVGTQEQPPVQCRGRGREAKRIRCTRPAPASPAVSSAVYVDRSSLWLAAAVAAGYLLPCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QNF4</accession>
    <name>A0A0Q3QNF4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_2g04935v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03002.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3QNF4"/>
    <dbReference type="EnsemblPlants" id="KQK03002">
        <property type="protein sequence ID" value="KQK03002"/>
        <property type="gene ID" value="BRADI_2g04935v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03002">
        <property type="protein sequence ID" value="KQK03002"/>
        <property type="gene ID" value="BRADI_2g04935v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3QNF4"/>
    <dbReference type="OrthoDB" id="775830at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF128">
        <property type="entry name" value="ASPARTIC PROTEINASE CDR1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="8">
        <location>
            <begin position="125"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="476"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK03002.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="504" mass="52626" checksum="6E0F9CACCC92AA88" modified="2018-03-28" version="2">MSPGGAAAAPASGSPRPPSAPASISLPPPRPPGCRPPLLLAPASNHGSEAKWFSVEFIHRDSVKSLFHDPTLTPEARLRQAARRSMARHAHAARINNSAAAAGASGSDDSDADVVSPMVPQNFEYLMALDVSTPPVRMLALADTGSSLVWLKCKLPAAHTPAATNGASSSYARLPCDAFACKALGDAASCRATGSGNNICVYRYAFADGSCTAGPVTVDAFTFSTLRKEKKQPTSVPRLDFGCATRTEGLSVPDDGLVGLANGPISLVSQLSAKTPFAHKFSYCLVPYSSSETVSSSLNFGSHAIVSSSPGAATTPLVAGRNKSFYTIALDSIKVAGKPVPLQTTTTKLIVDSGTMLTYLPKAVLDPLVAALTAAIKLPRVKSPETLYAVCYDVRRRAPEDVGKSIPDVTLVLGGGGEVRLPWGNTFVVENKGTTVCLALVESHLPEFILGNVAQQNLHVGFDLERRTVSFAPAIARAPATPPPPHTLITSRGRTSGLSEIGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QUG4</accession>
    <name>A0A0Q3QUG4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor TFIIB cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104583107</name>
        <name evidence="3" type="ORF">BRADI_2g17760v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3QUG4"/>
    <dbReference type="STRING" id="15368.A0A0Q3QUG4"/>
    <dbReference type="EnsemblPlants" id="KQK05066">
        <property type="protein sequence ID" value="KQK05066"/>
        <property type="gene ID" value="BRADI_2g17760v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05066">
        <property type="protein sequence ID" value="KQK05066"/>
        <property type="gene ID" value="BRADI_2g17760v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="704629at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
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        <source>
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    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
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                <person name="Mockler T.C."/>
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                <person name="Rokhsar D."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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        </source>
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        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Rokhsar D."/>
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        </source>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Bevan M.W."/>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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        </source>
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    </dbReference>
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        <property type="gene ID" value="BRADI_4g07568v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT62723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT63203"/>
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    </dbReference>
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        <property type="protein sequence ID" value="PNT63203"/>
        <property type="gene ID" value="BRADI_4g12817v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CME6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <end position="227"/>
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            <end position="246"/>
        </location>
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            <begin position="1"/>
            <end position="62"/>
        </location>
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            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="363" mass="39977" checksum="258465DE5173475F" modified="2018-03-28" version="1">MAEIEGDGRDAYVHHQAPTRKRAPPEVTRLATPATVAISPLPCPSSHQPHPRIPTPASSTPVRFSWADACDEEEEEEEFVPDSLPPPPLVPCRLDSLTSSMQPDLVGRGSYGHPDGFCRLVLLARLPMDSPASRAALRPRAHPPPIHSSAACHVPARAARPDGDSSHHEEGWQLVRRPHWWRRRNQGAPQLASTKGKEERRAALLKRMRGRCFRCLSQRHKASGCREPQHCWKCHSPGHLESACLAVPVSPPPCPRSSRPHDHFRILVVLARISPGPVHLLEHFMVGSHLGKAKLGGVGFGPTDGTVIRHGARLSPEGAVYGLKLREASSIQIILRLKQNTTRFLGVRLANEELDGEKHIQCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CPN2</accession>
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            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">BRADI_4g22815v3</name>
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    <organism evidence="2">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="18"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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        </location>
    </feature>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="PNT63963.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT63963"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
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            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT64783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_4g32945v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_4g32945v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT64783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="85" mass="10048" checksum="5AD05A6D52A04D6F" modified="2018-03-28" version="1">MQQPRMAGRCRVLLVHCCPRDRGEQTEEEQMKEARSPPSPSWPTSTVMIKIHLYLQYSSPIRGTMRDAWFVDLSANNFFYVLYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CTU8</accession>
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            <fullName evidence="5">RING-type domain-containing protein</fullName>
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        <name evidence="6" type="ORF">BRADI_4g42671v3</name>
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    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A2K2CTU8">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CTU8"/>
    <dbReference type="EnsemblPlants" id="PNT65451">
        <property type="protein sequence ID" value="PNT65451"/>
        <property type="gene ID" value="BRADI_4g42671v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65451">
        <property type="protein sequence ID" value="PNT65451"/>
        <property type="gene ID" value="BRADI_4g42671v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CTU8"/>
    <dbReference type="OrthoDB" id="696294at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger, C3HC4 type family protein, expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF23">
        <property type="entry name" value="OS12G0134500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="114"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT65451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="165" mass="18071" checksum="A9113C8F2CA43861" modified="2018-03-28" version="1">MTARGVSLDWVIIPIVGAVVISRRALPWTYGVTASALQRIEDEDNDEIVTMEEEGRYLREVLGMEDEDDDYGDADVMMDVAYANGGFGAVPASGESVSTLETRKHDGSGGDGGCVICLEEYEVGEELSVVPCEHRHEFHRSCLEQWLALSRLCPLCRHALPAGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CW11</accession>
    <name>A0A2K2CW11_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_3g08655v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CW11"/>
    <dbReference type="EnsemblPlants" id="PNT66214">
        <property type="protein sequence ID" value="PNT66214"/>
        <property type="gene ID" value="BRADI_3g08655v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66214">
        <property type="protein sequence ID" value="PNT66214"/>
        <property type="gene ID" value="BRADI_3g08655v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CW11"/>
    <dbReference type="OrthoDB" id="694273at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06222">
        <property type="entry name" value="RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044730">
        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052929">
        <property type="entry name" value="RNase_H-like_EbsB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074">
        <property type="entry name" value="BNAC02G40300D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074:SF11">
        <property type="entry name" value="REVERSE TRANSCRIPTASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="154"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT66214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="303" mass="32994" checksum="F7436BF5CB16AD43" modified="2018-03-28" version="1">MDHSSTCAICGSEPETSFHAVMSCTKARALRFDIRNSWKLPAEKELKFTGPDWVLLILANSSQDTRRNLPLLWWRAWHLRNGIILKGQATVAASVTFLLKFSQETATSFTTEIPDQKGKRPIGVTTPAASTKQAVSSSGSGWTAPGDPFLKLNVDDGFVASSEEASWGAVLRNHLGEALLSAWGRLPYCCSAEETELAACIEGLRISRAARTTENILESDCQMMTNALNQVHRNHSHLAAVVHDAKVLMIEPLSCSVAFVSRGNNSVAHALAMKGKTSRCNRLVLEDVPPDVRPLALGDLMPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CXN1</accession>
    <name>A0A2K2CXN1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">transaldolase</fullName>
            <ecNumber evidence="3">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100844483</name>
        <name evidence="7" type="ORF">BRADI_3g16927v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transaldolase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573490.2">
        <property type="nucleotide sequence ID" value="XM_003573442.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CXN1"/>
    <dbReference type="FunCoup" id="A0A2K2CXN1">
        <property type="interactions" value="2603"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CXN1"/>
    <dbReference type="EnsemblPlants" id="PNT66787">
        <property type="protein sequence ID" value="PNT66787"/>
        <property type="gene ID" value="BRADI_3g16927v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844483"/>
    <dbReference type="Gramene" id="PNT66787">
        <property type="protein sequence ID" value="PNT66787"/>
        <property type="gene ID" value="BRADI_3g16927v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844483"/>
    <dbReference type="OrthoDB" id="2015515at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CXN1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00957">
        <property type="entry name" value="Transaldolase_TalAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000163">
        <property type="entry name" value="Transaldolase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004730">
        <property type="entry name" value="Transaldolase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF18">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT66787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="429" mass="46501" checksum="48D54494B5135819" modified="2018-03-28" version="1">MRLGDPVCDCLLFFFFSSAAPKPPPIPARIHPMALSVSAPASSSFLPSSRQVGGRWSSPASSARPVASSLRRPVLAARAAAGNVPSSPVDQVVTELDVVSSFSEIVPDTVVFDDFEKFAPTAATVSSSLLLGIAGLPDTKFKSAIDTALADGECNTMENSGDRMSCFLTKALGNVGAELAHQVPGRVSTEIDSRLAYDTQGIIQRVHELLQIYNEHDVPSERLLFKIPATWQGIEASRLLESEGTQTHLTFVYSFAQAAAAAQAGASVVQIFVGRIRDWARNHSGDPEIDEALRKGEDAGLALVKKVYAYIHKNGYRTKLMAAAVRNKQDVFSLLGIDYIITPLKILQSLDESVTDPDEKYGYVQRLTPSLDKMYNFSEEELLKWDQLSLAAAMGPAAEDLLASGLEGYADQARRVEELFGKIWPPPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CXP8</accession>
    <name>A0A2K2CXP8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g17303v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
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            <begin position="1"/>
            <end position="10"/>
        </location>
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        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="83" mass="8835" checksum="6CC79C63847D47ED" modified="2018-03-28" version="1">MLDRNTQSVPNPRFGGRRIKARPSSPSSRTSLLPRRTSPPCPHSLLPPPRSPPSSPPPSLPPQQRPPLSPTISPIPISPAPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CZE5</accession>
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            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g27069v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67404">
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        <property type="gene ID" value="BRADI_3g27069v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g27069v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g27069v3"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="PNT67403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="95" mass="10317" checksum="063394EC7B3FD267" modified="2018-03-28" version="1">MLKDVAVPLFSNAPNNIYEKTVEVPLPVSIEPNSIQFPPLINGGGSFDHGMGRANQNLTESHTFLVKGQALQRWSMVSEPGCRAHITLHGPILSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D0G1</accession>
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        <lineage>
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            <taxon>BOP clade</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT67768">
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    </dbReference>
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        <property type="gene ID" value="BRADI_3g31973v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
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    </dbReference>
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        <location>
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            <end position="90"/>
        </location>
    </feature>
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        <location>
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            <end position="185"/>
        </location>
    </feature>
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        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT67768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
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    <name>A0A2K2D0P7_BRADI</name>
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            <fullName evidence="1">Neprosin PEP catalytic domain-containing protein</fullName>
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    </protein>
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        <name evidence="2" type="ORF">BRADI_3g32845v3</name>
    </gene>
    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A2K2D0P7"/>
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        <property type="protein sequence ID" value="PNT67847"/>
        <property type="gene ID" value="BRADI_3g32845v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67847">
        <property type="protein sequence ID" value="PNT67847"/>
        <property type="gene ID" value="BRADI_3g32845v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="609523at2759"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
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        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF211">
        <property type="entry name" value="OS06G0682600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
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    </dbReference>
    <dbReference type="Pfam" id="PF03080">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="25"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="280" mass="30324" checksum="FE96EEB11875061A" modified="2018-03-28" version="1">MSNGQDLAPDDDGAVMSYVSTTACSSFLNNFIYAAHHTQERASGYYGLVATMDVYGHNLNVDQLTMGAIWIINSNGHVSNVNAITVGWLVWPSHFNDSRTYLFTEWINDSNGQTKGCMNGDCNPGFQFVSGSPIFPGDVLDPVSQPNNARQNLTIKVFKEKSMGYWWVHCGFNSDPVPVGFFRGTLFDSLSSKATKILVGGYTTKYKKDVPSPPMGSGASASSDTRKAALVRDIQFIDEDGNSTPIGDDDMLATIVDNRLYFALPIAGGQFSYGGPGGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D2L5</accession>
    <name>A0A2K2D2L5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g41993v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D2L5"/>
    <dbReference type="EnsemblPlants" id="PNT68534">
        <property type="protein sequence ID" value="PNT68534"/>
        <property type="gene ID" value="BRADI_3g41993v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68534">
        <property type="protein sequence ID" value="PNT68534"/>
        <property type="gene ID" value="BRADI_3g41993v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D2L5"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT68534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="80" mass="8991" checksum="884169425B1C4FEE" modified="2018-03-28" version="1">MQNWGEDIDPLQKRKMPTKASLLLRCRRSLLASGARGLLAGVGKQRSSKMKKKILFLVDFSETWWNDTAQSKVGLSLSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D3D2</accession>
    <name>A0A2K2D3D2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH-plastoquinone oxidoreductase subunit K</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g45403v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D3D2"/>
    <dbReference type="STRING" id="15368.A0A2K2D3D2"/>
    <dbReference type="EnsemblPlants" id="PNT68804">
        <property type="protein sequence ID" value="PNT68804"/>
        <property type="gene ID" value="BRADI_3g45403v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68804">
        <property type="protein sequence ID" value="PNT68804"/>
        <property type="gene ID" value="BRADI_3g45403v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D3D2"/>
    <dbReference type="OrthoDB" id="1889813at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT68804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13108" checksum="50B70CE7BA253603" modified="2018-03-28" version="1">MVLTEYLEKKKEEKDSIETVMSLIEFPLLDQTSSNSVISTTPNDLSNWSRLSSLWPLLYGTSCCFIEFASLIGSRFDFDRYGLVPRSSPRQADLILTAGTVTMKMAPFDDELMIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D3E3</accession>
    <name>A0A2K2D3E3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g45245v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D3E3"/>
    <dbReference type="EnsemblPlants" id="PNT68790">
        <property type="protein sequence ID" value="PNT68790"/>
        <property type="gene ID" value="BRADI_3g45245v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68790">
        <property type="protein sequence ID" value="PNT68790"/>
        <property type="gene ID" value="BRADI_3g45245v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D3E3"/>
    <dbReference type="OrthoDB" id="691879at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT68790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="98" mass="10879" checksum="65345995F1943548" modified="2018-03-28" version="1">MISTHPFLSSYVPYISLLDQGLSWSSAPMELCCREGPEGGTVPRGKKPKKLLEMPLASGLPLNGRVPLDAWQLDTSNRSTRRNHHGCTHGLLLLRAWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D425</accession>
    <name>A0A2K2D425_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100831752</name>
        <name evidence="8" type="ORF">BRADI_3g48271v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D425"/>
    <dbReference type="FunCoup" id="A0A2K2D425">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2D425"/>
    <dbReference type="EnsemblPlants" id="PNT69029">
        <property type="protein sequence ID" value="PNT69029"/>
        <property type="gene ID" value="BRADI_3g48271v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69029">
        <property type="protein sequence ID" value="PNT69029"/>
        <property type="gene ID" value="BRADI_3g48271v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="645822at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="130"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="7">
        <location>
            <begin position="228"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="235"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT69029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="330" mass="36750" checksum="BE8A53B42CF94E98" modified="2018-03-28" version="1" fragment="single">QEVFDAYLRSIGVVLPSLPPVEFAPAPVHHAADAKIEHTVKPRASCELYAAAMGARRAQRAVEVPVLPDPKIGHSKNSKTRRRLCPDYEDDIDANLKMMEKNAGERPLPDYLTTVQGDKISPLTRGALVLWMDKLGRHYNLAPGTLHLAVACIDRFLSVRTARNYRAYEFQLLGATAVFTAAKYEDQSTQHKLNTAEIARYCGLETSEEVRETERDMMKALGFQISGPTAYTFVGHFTRYFSQGKEELRVQRLAHRIADQSLLSHVCVGFLPSVVASSAIFLARFALNPSGVLPWNAELQELTGYDSLDLSSCVQIMYSFSQSLICNPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D4V7</accession>
    <name>A0A2K2D4V7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g53334v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D4V7"/>
    <dbReference type="EnsemblPlants" id="PNT69312">
        <property type="protein sequence ID" value="PNT69312"/>
        <property type="gene ID" value="BRADI_3g53334v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69312">
        <property type="protein sequence ID" value="PNT69312"/>
        <property type="gene ID" value="BRADI_3g53334v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D4V7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT69312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="79" mass="9288" checksum="5FAC9C3C7D4CA362" modified="2018-03-28" version="1">MDKGSCTPTYTKEQERIYTQELGRSSYKETNCTKDWGSTKQKERNFLQHWRKPEFTDKLPANQNDKVATFSLCFQPGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DDG4</accession>
    <name>A0A2K2DDG4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g42736v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DDG4"/>
    <dbReference type="EnsemblPlants" id="PNT72323">
        <property type="protein sequence ID" value="PNT72323"/>
        <property type="gene ID" value="BRADI_2g42736v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72323">
        <property type="protein sequence ID" value="PNT72323"/>
        <property type="gene ID" value="BRADI_2g42736v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DDG4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT72323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="75" mass="8175" checksum="AA89862C44E1A6C4" modified="2018-03-28" version="1">MSRCPGDRTPQASPLSSEAPILTSLLSLPSLRLARDTLSACFSSSRRSNGRGSPMVFLLPPPRTYNLLVVFVEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DEW4</accession>
    <name>A0A2K2DEW4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein cereblon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein ohgata</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100828195</name>
        <name evidence="10" type="ORF">BRADI_2g49550v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Has an essential role in mediating growth by negatively regulating insulin signaling. It also has a role in maintaining presynaptic function in the neuromuscular junction synapses of third-instar larvae.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Likely a component of a DCX (DDB1-CUL4-X-box) protein ligase complex. May interact with pic/DDB1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRBN family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DEW4"/>
    <dbReference type="STRING" id="15368.A0A2K2DEW4"/>
    <dbReference type="EnsemblPlants" id="PNT72836">
        <property type="protein sequence ID" value="PNT72836"/>
        <property type="gene ID" value="BRADI_2g49550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72836">
        <property type="protein sequence ID" value="PNT72836"/>
        <property type="gene ID" value="BRADI_2g49550v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="267517at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DEW4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15777">
        <property type="entry name" value="CRBN_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000005">
        <property type="entry name" value="Blast:Protein cereblon homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000007">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034750">
        <property type="entry name" value="CULT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255">
        <property type="entry name" value="CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255:SF23">
        <property type="entry name" value="OS01G0743600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51788">
        <property type="entry name" value="CULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="8">
        <location>
            <begin position="92"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="CULT" evidence="9">
        <location>
            <begin position="366"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT72836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="497" mass="55957" checksum="095087040DFD6ECC" modified="2018-03-28" version="1">MAEQPAWMRRQMEQILELDMEELEVEEVDDSASSSSSDVSTFLRNTHGGGETGTSEEVTINTSRASLHTYVGEVVDTRSRFAFLNGGEVLNLPMLYLQGFVLFPEATLTLRVIQPRFLATVDKAINHVDAPCMIGVVHVYRHINDGLHSVASIGTTAEIHQVRQSGDGTSNVSTRGQQRFRLRRSWIDADEVPWGEVQIIEEDTPSRTPRNAFVQLAANTGMCYFSNEDEDLMRELSWQNHDCINEFDASVNKTNMGDDDDLRFAERQRQYRAACNSKMALEAPLSFWPRWAYKMYDSYSLARRAADLWRQIVANPSMDDHVRKPDLLSFCIGSKLPISGSARQELLEIDGISYRLQREIQLLKAFNLMRCRNCLVLIARRSDMVVISNGCPVGAYVKPFDGAQEMITVYNASGLALYGNPSEVHSWFPGYTWTIALCAACESNIGWLFSAAKKNLVPKSFWGQPTRCWPDINPAKKGNYSARAHNGSKQGSRSLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DN38</accession>
    <name>A0A2K2DN38_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g36782v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DN38"/>
    <dbReference type="EnsemblPlants" id="PNT75693">
        <property type="protein sequence ID" value="PNT75693"/>
        <property type="gene ID" value="BRADI_1g36782v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75693">
        <property type="protein sequence ID" value="PNT75693"/>
        <property type="gene ID" value="BRADI_1g36782v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DN38"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT75693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="249" mass="27324" checksum="81CBAA6B1D9E5BF9" modified="2018-03-28" version="1" fragment="single">QRTSTSPRRIRLPPHTAPPLRPPPWPRASISPRHICRPPLTTPPLCPSQPSTLCPLLSRGWPATDPPGASQRPRRSSEQRGDKGGRRGRRIDVQLVWTNQAGAPYPPIFLIRGGRRSHGAFSSPPPPAQLSSSRPPTPVKSSPPRGIKSCTKIIFRSIPAELWSLKSLISLDFYNSYICRDNTKRTQQLKSLVFLSDILLTALILLASPPVKSPPPHAPVSNPPPPLPAPVSSPPPRVKLHAYRNPQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DN51</accession>
    <name>A0A2K2DN51_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g36752v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DN51"/>
    <dbReference type="EnsemblPlants" id="PNT75689">
        <property type="protein sequence ID" value="PNT75689"/>
        <property type="gene ID" value="BRADI_1g36752v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75689">
        <property type="protein sequence ID" value="PNT75689"/>
        <property type="gene ID" value="BRADI_1g36752v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DN51"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT75689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="140" mass="13939" checksum="B5B09A0BA698FEA4" modified="2018-03-28" version="1">MAESGESSRAATSAVQHTAAAAVAPMPTTGQEVISGVKIPDAQHTANADGGSLLHTGDQMMAVAQLVPSFSLDSSGDQVAASNLLVASDKTMQASAGSTSTMGNHGSISIGTMLTGSATTSSPAKVQIYVLVCANKIMIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DTI9</accession>
    <name>A0A2K2DTI9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g65571v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DTI9"/>
    <dbReference type="FunCoup" id="A0A2K2DTI9">
        <property type="interactions" value="3140"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DTI9"/>
    <dbReference type="EnsemblPlants" id="PNT77596">
        <property type="protein sequence ID" value="PNT77596"/>
        <property type="gene ID" value="BRADI_1g65571v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77596">
        <property type="protein sequence ID" value="PNT77596"/>
        <property type="gene ID" value="BRADI_1g65571v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DTI9"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DTI9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF10">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHATASE SAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="138"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT77596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="584" mass="65168" checksum="3A8161853665A35C" modified="2018-03-28" version="1">METDADEAPLLAEEPLRPGSCSSELELREFRDRYVIRSLDGGGAFAVARSDGSLRPLSPEEAASGSDCKISRIYGVAGMIRMLAGSYILVITSRKDAGSYQASTVYHVNSMKFLCCNEAIKHLTSQEKRDEAYFMSLLRIAETTCGLYYSYDRDLTLNLQRASKLVAGRVHKPLWKQADPRFVWNRNLLEELIEAKTAQFTLKHGPVRITLFSRRCNRRLGTRMWRRGANLEGATANFVETEQLVEYEGLTSSFIQVRGSIPLLWEQIVDLSYKPRPSIIEHDEMTKVVQRHFHDLSQRYGDTMVVDLTDKQGDEGNLSNAFAAEMEKFPNIRYVHFDFHHICGGGNFHNLQALYDEVEETIHKQGYFLMNSKGEILLDQSGVVRSNCIDCLDRTNVTQSFLARKSLDSQLQRMGALSSAESISQSDGINDTFKKLWVEHGDELSLEYAGSYALKGDLVRYGRQTLPGLIKDGMSALSRYYLNNFHDGVRQDALDLISGYYTVSKSSSSPFQIVGFESAPYLPVASAIIVGGITVTTFTLSQVGRSAQHLISSIICAGLTAGVVALVKANGKQFCSRPRLCGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DUU0</accession>
    <name>A0A2K2DUU0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein BIG GRAIN 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_1g72920v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BIG GRAIN 1 (BG1) plant protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DUU0"/>
    <dbReference type="FunCoup" id="A0A2K2DUU0">
        <property type="interactions" value="760"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DUU0"/>
    <dbReference type="EnsemblPlants" id="PNT78047">
        <property type="protein sequence ID" value="PNT78047"/>
        <property type="gene ID" value="BRADI_1g72920v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78047">
        <property type="protein sequence ID" value="PNT78047"/>
        <property type="gene ID" value="BRADI_1g72920v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DUU0"/>
    <dbReference type="OrthoDB" id="680041at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DUU0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060918">
        <property type="term" value="P:auxin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009630">
        <property type="term" value="P:gravitropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010929">
        <property type="term" value="P:positive regulation of auxin mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080113">
        <property type="term" value="P:regulation of seed growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039621">
        <property type="entry name" value="BG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33541:SF28">
        <property type="entry name" value="PROTEIN BIG GRAIN 1-LIKE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33541">
        <property type="entry name" value="PROTEIN BIG GRAIN 1-LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="71"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT78047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="34276" checksum="64B9944350BBBB21" modified="2018-03-28" version="1">MERRGEKGAAAARARRPQGAAEQPSFSSTLLDAIYKSMDEPGHDAVSKKKQQEKEEALHYSYYYRPSLAGSYRARAPGPAHATTTTSSSSDCSSYGGFSSSEAETSSGRHHHRRLRPIRTAAAAPAPPAPEKKASKKQQVAPGASIRAKLRDLRKAPASPGARLAGFLNTIFAGGGGKRAPQTPPSASAAAEYACSTASSAASYSRSCLSKTPSTRGGGGQQQAGRTVRFVDSAAEAPATVPGRRMPARAVEQMLLRRMEMESDEEDEESSDASSDLFELENFTAAPPGAAGDELPVYETTRVVLNRGSIGGHHGYGHGRSARVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLM3</accession>
    <name>I1GLM3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100842729</name>
        <name evidence="14" type="ORF">BRADI_1g03947v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563753.1">
        <property type="nucleotide sequence ID" value="XM_003563705.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLM3"/>
    <dbReference type="STRING" id="15368.I1GLM3"/>
    <dbReference type="EnsemblPlants" id="KQK12471">
        <property type="protein sequence ID" value="KQK12471"/>
        <property type="gene ID" value="BRADI_1g03947v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842729"/>
    <dbReference type="Gramene" id="KQK12471">
        <property type="protein sequence ID" value="KQK12471"/>
        <property type="gene ID" value="BRADI_1g03947v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842729"/>
    <dbReference type="eggNOG" id="ENOG502QTQP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020953_3_1_1"/>
    <dbReference type="OMA" id="ATMMLEM"/>
    <dbReference type="OrthoDB" id="1932925at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GLM3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990538">
        <property type="term" value="F:xylan O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045492">
        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF67">
        <property type="entry name" value="XYLAN O-ACETYLTRANSFERASE 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093929" evidence="11">
        <location>
            <begin position="21"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="13">
        <location>
            <begin position="41"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="12">
        <location>
            <begin position="96"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK12471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="391" mass="43465" checksum="A13D24D546069467" modified="2012-06-13" version="1">MAKTLLAILALAAPFLLAAAQGEGGGRPERLPFAVGASPEGCDVGVGEWVRDEAARPLYEEASCPYIPQELTCQAHGRPDAAYQNWRWQPRGCGELPSFNATVMLEMLRGKRMLFVGDSLNRGQYVSLLCLLHRAIPEGSRSFETIDALSIFRAKDYDATIEFYWAPLLAESNSDAAVEHRLEERVIRGAPMDRHSRFWKGADVLVFNSYLWWTTGTKIQILRGADNDMSKDIVEMPAEEAYRLVLHQVANWLDANVDPSKSRVLFVTASPTHGGGKGNDCYGQKTPITGDEHASYTSRAMLKVADEVLGGSRSRRAVVPVGVVNVTRMSEYRRDAHTQVYREQWGKTGVAGKQEQSDADCTHWCLPGVPDAWNELLYWKLFFPAADDQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMA8</accession>
    <name>I1GMA8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Photosystem I iron-sulfur center</fullName>
            <ecNumber evidence="3">1.97.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">9 kDa polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">PSI-C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Photosystem I subunit VII</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">PsaC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">BRADI_1g05756v3</name>
    </gene>
    <organism evidence="28">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">Bd21</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Bd21</strain>
        </source>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="28">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Apoprotein for the two 4Fe-4S centers FA and FB of photosystem I (PSI); essential for photochemical activity. FB is the terminal electron acceptor of PSI, donating electrons to ferredoxin. The C-terminus interacts with PsaA/B/D and helps assemble the protein into the PSI complex. Required for binding of PsaD and PsaE to PSI. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>reduced [plastocyanin] + hnu + oxidized [2Fe-2S]-[ferredoxin] = oxidized [plastocyanin] + reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:30407"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10039"/>
            <dbReference type="Rhea" id="RHEA-COMP:10040"/>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
            <dbReference type="ChEBI" id="CHEBI:30212"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="EC" id="1.97.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="25">The eukaryotic PSI reaction center is composed of at least 11 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="24">Plastid thylakoid membrane</location>
            <topology evidence="24">Peripheral membrane protein</topology>
            <orientation evidence="24">Stromal side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.97.1.12" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMA8"/>
    <dbReference type="FunCoup" id="I1GMA8">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GMA8"/>
    <dbReference type="EnsemblPlants" id="KQK12746">
        <property type="protein sequence ID" value="KQK12746"/>
        <property type="gene ID" value="BRADI_1g05756v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12746">
        <property type="protein sequence ID" value="KQK12746"/>
        <property type="gene ID" value="BRADI_1g05756v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S26M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_8_0_1"/>
    <dbReference type="InParanoid" id="I1GMA8"/>
    <dbReference type="OMA" id="GHMSHAV"/>
    <dbReference type="OrthoDB" id="1865383at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009522">
        <property type="term" value="C:photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055035">
        <property type="term" value="C:plastid thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009773">
        <property type="term" value="P:photosynthetic electron transport in photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000001">
        <property type="entry name" value="Photosystem I iron-sulfur center"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01303">
        <property type="entry name" value="PSI_PsaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050157">
        <property type="entry name" value="PSI_iron-sulfur_center"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017491">
        <property type="entry name" value="PSI_PsaC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03048">
        <property type="entry name" value="PS_I_psaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960:SF79">
        <property type="entry name" value="PHOTOSYSTEM I IRON-SULFUR CENTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960">
        <property type="entry name" value="PHOTOSYSTEM I IRON-SULFUR CENTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="12" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="17" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="26">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="26">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00063727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KQK12746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="81" mass="8889" checksum="AB1F63DC8FC601D2" modified="2012-06-13" version="1">MSHSVKIYDTCIGCTQCVRACPTDVLEMIPWDGCKAKQIASAPRTEDCVGCKRCESACSTDFLSVRVYLGPETTRSMALSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMZ2</accession>
    <name>I1GMZ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100831587</name>
        <name evidence="8" type="ORF">BRADI_1g07640v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558997.1">
        <property type="nucleotide sequence ID" value="XM_003558949.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMZ2"/>
    <dbReference type="STRING" id="15368.I1GMZ2"/>
    <dbReference type="EnsemblPlants" id="KQK13042">
        <property type="protein sequence ID" value="KQK13042"/>
        <property type="gene ID" value="BRADI_1g07640v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831587"/>
    <dbReference type="Gramene" id="KQK13042">
        <property type="protein sequence ID" value="KQK13042"/>
        <property type="gene ID" value="BRADI_1g07640v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831587"/>
    <dbReference type="eggNOG" id="KOG1946">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046065_0_1_1"/>
    <dbReference type="OMA" id="FSMNKYI"/>
    <dbReference type="OrthoDB" id="10251073at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000500">
        <property type="term" value="C:RNA polymerase I upstream activating factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10567">
        <property type="entry name" value="SWIB-MDM2_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.245.10:FF:000004">
        <property type="entry name" value="Upstream activation factor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DEK-C" evidence="7">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="DM2" evidence="6">
        <location>
            <begin position="137"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="DM2" evidence="6">
        <location>
            <begin position="253"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="213"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK13042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="37637" checksum="64960B819D47AED7" modified="2012-06-13" version="1">MVSDSELVERLREVLKDSDLTTTTTGALRRRLEEDFGVDLSDKKAFVREQVDILLSEFSDKAEQEDVAAPEEEEPEERVPEPEGGEGSGVDGEEEEEPEEVEEEEDEDEEDGDSSGGHKRKRRLDKGNSDGKRKGGGFTKLCSISPTLQEFVGASELARTEVVKKLWAYIRENNLQDPSNKRKILCDETLKKLFKVNSIDMFQMNKALTKHIWPLNSEGPASPKKSTPKEKPQKREKNEGKKQKVGSSRPGTGLNAPLQLSDDLANFIGTGESMLSRSDVVKIMWDYIKENNLQDPSDRRKIICDEKLKNLFQVDSFTGFTVSKLLSPHFTKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1GP09</accession>
    <name>I1GP09_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pinin/SDK/MemA protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100834865</name>
        <name evidence="11" type="ORF">BRADI_1g10810v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pinin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560660.1">
        <property type="nucleotide sequence ID" value="XM_003560612.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GP09"/>
    <dbReference type="FunCoup" id="I1GP09">
        <property type="interactions" value="396"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GP09"/>
    <dbReference type="EnsemblPlants" id="KQK13537">
        <property type="protein sequence ID" value="KQK13537"/>
        <property type="gene ID" value="BRADI_1g10810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834865"/>
    <dbReference type="Gramene" id="KQK13537">
        <property type="protein sequence ID" value="KQK13537"/>
        <property type="gene ID" value="BRADI_1g10810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834865"/>
    <dbReference type="eggNOG" id="KOG3756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044657_0_0_1"/>
    <dbReference type="OMA" id="WKANRRQ"/>
    <dbReference type="OrthoDB" id="330772at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039853">
        <property type="entry name" value="Pinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006786">
        <property type="entry name" value="Pinin_SDK_MemA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12707:SF0">
        <property type="entry name" value="PININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12707">
        <property type="entry name" value="PINN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04696">
        <property type="entry name" value="Pinin_SDK_memA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Pinin/SDK/MemA protein" evidence="10">
        <location>
            <begin position="150"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="88"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="172"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="379"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK13537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="404" mass="46201" checksum="A9A67B756806D874" modified="2012-06-13" version="1">MAAATGKTAEEIRRELQELQRQHREISERLRDPRGLRRGAPAAGPGAARPLRGFARPAAESGDQPEPKRRLLSAVVKVDGAEVKDDFANDPVMEGLEDGSTAAEGGERRGVSNGGFRRDGSQWVSRRELNNALPEPLPREFPKDEDQSLVRRNKRMLGKLLVGTLEKFQQEDKKLSNSEAYMRRSEAQLKADQKAREDSERLRQQEREQAAEKRRRDMTLRARVAAKAEEKRLELLYIQWAEHHKKLSNFLRTKTEPPIYYTMAKPIIDDPAIVERNKEKVFEEWKTVRRAELTQFQKQVEEQYLSNVERQLERMQNARNARRGNGPANMQEMDKELDTHRAEHGPKARRILDGGIDDEEEVEDMAAEDDLMDEVLGVNEAINEDPTKPSDEATDVTPIPDKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQY2</accession>
    <name>I1GQY2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Nicotianamine synthase</fullName>
            <ecNumber evidence="2 6">2.5.1.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100845767</name>
        <name evidence="7" type="ORF">BRADI_1g17090v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Synthesizes nicotianamine, a polyamine which serves as a sensor for the physiological iron status within the plant, and/or might be involved in the transport of iron.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3 S-adenosyl-L-methionine = nicotianamine + 3 S-methyl-5'-thioadenosine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16481"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:58249"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.43"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the nicotianamine synthase (NAS)-like family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.43" evidence="2 6"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752314.1">
        <property type="nucleotide sequence ID" value="XM_014896828.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQY2"/>
    <dbReference type="EnsemblPlants" id="KQK14538">
        <property type="protein sequence ID" value="KQK14538"/>
        <property type="gene ID" value="BRADI_1g17090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845767"/>
    <dbReference type="Gramene" id="KQK14538">
        <property type="protein sequence ID" value="KQK14538"/>
        <property type="gene ID" value="BRADI_1g17090v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTU6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031919_0_0_1"/>
    <dbReference type="OMA" id="DTIFHNF"/>
    <dbReference type="OrthoDB" id="1858069at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030410">
        <property type="term" value="F:nicotianamine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030418">
        <property type="term" value="P:nicotianamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004298">
        <property type="entry name" value="Nicotian_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266:SF12">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03059">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51142">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK14538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="375" mass="39552" checksum="E8081BC0C59DB98D" modified="2012-06-13" version="1">MGMEAKPTPAAISEDESALVQKITKLATAIGDLPSLHPSPEVNSLFTELVTACIPPSTIDVDTLGPEAQSMRRRLITLCADAEGHLESHYSDLLAAHDGDPLDHLEIFPYFGNYVKLSQLEHGLLSRHVSESFSRVAFLGSGPLPLSSLVLAARHLPDAAFVNYDISPDANARASRLVHADADADAGIGIGARMEFRTADVTDEAVAGEMGRYDVVFLAALVGMAAEEKARVVAHLGRHMAPGAALVVRSAHGARGFLYPVVDPEDVRKGGFEVLCVHHPEDEVINSVIIAQKKMLEVDDEGCVRAENGSGIKGCAAAASENGSGMKGCAAAANGDVLCAAAAAVVSRPCLGCCEMEARAHRKLEEMAMDQELPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRQ8</accession>
    <name>I1GRQ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100837542</name>
        <name evidence="3" type="ORF">BRADI_1g19540v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562559.1">
        <property type="nucleotide sequence ID" value="XM_003562511.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRQ8"/>
    <dbReference type="EnsemblPlants" id="KQK14922">
        <property type="protein sequence ID" value="KQK14922"/>
        <property type="gene ID" value="BRADI_1g19540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837542"/>
    <dbReference type="Gramene" id="KQK14922">
        <property type="protein sequence ID" value="KQK14922"/>
        <property type="gene ID" value="BRADI_1g19540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837542"/>
    <dbReference type="eggNOG" id="KOG2690">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033676_0_1_1"/>
    <dbReference type="OMA" id="HIFDRAK"/>
    <dbReference type="OrthoDB" id="73788at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF5">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="2">
        <location>
            <begin position="184"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK14922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="457" mass="49692" checksum="EC83F6216141AC12" modified="2012-06-13" version="1">MDFLFNSMLTPPDPSGEHPDSPPPEPEPAGSASSPPDSAPAGDDGGGWGFGGLLKTLTSQSETVLEAYRRDLAEFSTGLRRETDALREAAARAARDLPSSAHALDGLADIVAQGKDAIAQVAAASAPSPADGSSAAAADSGADPSSASGHLRYNRFEAQLRALQSDPATFAADPEDAEDFAAWRAAGFSVEEKQEEIEALCYESDALEALVDRLVPDSVDSEVFWARYFYRVHKLKQQEDARAKLVKRVIAQEEDEDLSWEVDDEVEEEPADKEVKEEGIKQAPTKEEMKHGVEEREIEKPVEESKVEAVEEVGVPVKEQKNADPQQPEVFGSSMVVVDKEEKEEATKSNAEESSGKKTVAEESRSSTGDDVAKEGAKHETSDSSKDSDYSIVSRQRTATEEDLEWDEIEDLGEHEEKKGSTHGSSPAPKEELRKRLSVAEDDEDLSWDIEDDDDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRY5</accession>
    <name>I1GRY5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100822592</name>
        <name evidence="9" type="ORF">BRADI_1g20250v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559914.1">
        <property type="nucleotide sequence ID" value="XM_003559866.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRY5"/>
    <dbReference type="EnsemblPlants" id="KQK15034">
        <property type="protein sequence ID" value="KQK15034"/>
        <property type="gene ID" value="BRADI_1g20250v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK15035">
        <property type="protein sequence ID" value="KQK15035"/>
        <property type="gene ID" value="BRADI_1g20250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822592"/>
    <dbReference type="Gramene" id="KQK15034">
        <property type="protein sequence ID" value="KQK15034"/>
        <property type="gene ID" value="BRADI_1g20250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15035">
        <property type="protein sequence ID" value="KQK15035"/>
        <property type="gene ID" value="BRADI_1g20250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822592"/>
    <dbReference type="eggNOG" id="KOG0048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028567_3_0_1"/>
    <dbReference type="OMA" id="ATDEQCN"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000158">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000394">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051953">
        <property type="entry name" value="Plant_SW-associated_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997:SF19">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="64"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="64"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK15035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="321" mass="33289" checksum="C1F4484ED2C95CED" modified="2012-06-13" version="1">MAKRPAGGGTKKRLRRGLWSPEEDEKLLNHIAKFGNGCWSSVPKIAGLERCGKSCRLRWINYLRPDLKRGAFSQQEEELIISLHSLLGNKWSQIAAQLPGRTDNEVKNFWNSFLKKKLRARGIDPATHKPLVSNLSPAPVFSDAAAELGLPASMAPPPPMPPLGVASAAAESACCYVYGGSSDCGGGGHGFMIPSVSSSSTLSSMAAGGAAAATEEQYSGGGFESSVTTQSSGGGGGAQLPWLELGGSISSYGGAGAGDEEQYGAALDELRWSDYVFDGGYQDQQCAAAAIYDHGKAGGDPAATAAQSFGAHGGGVSWCLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I1GX89</accession>
    <name>I1GX89_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g35850v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TSR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GX89"/>
    <dbReference type="STRING" id="15368.I1GX89"/>
    <dbReference type="EnsemblPlants" id="KQK17644">
        <property type="protein sequence ID" value="KQK17644"/>
        <property type="gene ID" value="BRADI_1g35850v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17644">
        <property type="protein sequence ID" value="KQK17644"/>
        <property type="gene ID" value="BRADI_1g35850v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1344904_0_0_1"/>
    <dbReference type="InParanoid" id="I1GX89"/>
    <dbReference type="OrthoDB" id="10469247at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019398">
        <property type="entry name" value="Pre-rRNA_process_TSR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21250">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094174" evidence="4">
        <location>
            <begin position="32"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="148"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK17644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="204" mass="22270" checksum="2979E54B5AE51E8A" modified="2012-06-13" version="1">MKPLGTSPSPGRLQLALLLIVLLIHRTHLLAADNLTAGAALRPPGYITCPSGDFAFGFRALDHEPSQFLLAVWFNLDLGKTSGDPALKKVVCHTKHGISSLVSIFLLKQVADQLLFIHEQCLQSNYSPIEKLKNSHVQENDVSQRRQILADDNDDDNVTSNDDDHTSTTDDEAAAAEETAVDSNPSPDADGWTVVPPRRHGRMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYG9</accession>
    <name>I1GYG9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g41720v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYG9"/>
    <dbReference type="EnsemblPlants" id="KQK18326">
        <property type="protein sequence ID" value="KQK18326"/>
        <property type="gene ID" value="BRADI_1g41720v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18326">
        <property type="protein sequence ID" value="KQK18326"/>
        <property type="gene ID" value="BRADI_1g41720v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071408_0_0_1"/>
    <dbReference type="InParanoid" id="I1GYG9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="43"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK18326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="173" mass="20140" checksum="244F2975FD465EC3" modified="2012-06-13" version="1">MAVADFLLFNATLLGPDPLLAESTRLAIEYLTQFILKHRDRHFVMIFYDLHGHWVTIAICLDMGRVTYFDPLRRKGNEPQRDFGPIKYVIDSAYRDAVKWYGMSGFSRSPNALLRHNFNFPCEQQHEGSVYCGYYCAHTIQQMINDFKPKGKKTFEKVRNWFLANAGTTLKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZS5</accession>
    <name>I1GZS5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100846893</name>
        <name evidence="13" type="ORF">BRADI_1g45800v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564091.1">
        <property type="nucleotide sequence ID" value="XM_003564043.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZS5"/>
    <dbReference type="STRING" id="15368.I1GZS5"/>
    <dbReference type="EnsemblPlants" id="KQK18991">
        <property type="protein sequence ID" value="KQK18991"/>
        <property type="gene ID" value="BRADI_1g45800v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846893"/>
    <dbReference type="Gramene" id="KQK18991">
        <property type="protein sequence ID" value="KQK18991"/>
        <property type="gene ID" value="BRADI_1g45800v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846893"/>
    <dbReference type="eggNOG" id="KOG0546">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_37_3_1"/>
    <dbReference type="OMA" id="EMEQNCN"/>
    <dbReference type="OrthoDB" id="407558at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GZS5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000009">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7 10" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="12">
        <location>
            <begin position="23"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="314"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="360"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK18991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="390" mass="42714" checksum="35324C8FAEC16F34" modified="2012-06-13" version="1">MEGGGGGVAPAAAAAEAKNPRCFMDVTIGGEMEGRIVIELYASVVPRTAENFRALCTGEKGVGASSGKPLHYKGSYFHRVIKGFMVQGGDFTAGDGTGGESIYGLKFEDENFVLKHERKGILSMANSGPNTNGSQFFITTTRTPHLDGKHVVFGRVIKGMGVVRSCEHIPVGDADRPTADAEIADCGELPEGADDGVVNFFKDGDMYPDWPNDLDEKPLEVSWWINAVEAAKTFGNDNFKKQDYKTALRKYRKALRYLDVCWEKEEIDEEKSSALRKTKSIILTNSSACKMKLGDLKGALLDADFALRETEGNAKAFFRQGQAHIALNDIDAAVESFQHALELEPNDGGIKRELAAAKKKIANRRDKERKAFSRMFQPSGKSDENNNENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I1H1I2</accession>
    <name>I1H1I2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g50690v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H1I2"/>
    <dbReference type="EnsemblPlants" id="KQK19825">
        <property type="protein sequence ID" value="KQK19825"/>
        <property type="gene ID" value="BRADI_1g50690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19825">
        <property type="protein sequence ID" value="KQK19825"/>
        <property type="gene ID" value="BRADI_1g50690v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R7Z7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097698_2_0_1"/>
    <dbReference type="InParanoid" id="I1H1I2"/>
    <dbReference type="OMA" id="LATPINC"/>
    <dbReference type="OrthoDB" id="719145at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36480:SF8">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN LEA-2 SUBGROUP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36480">
        <property type="entry name" value="OS06G0118900 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK19825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="21376" checksum="92ED88A14611FDD4" modified="2012-06-13" version="1">MAKVAPPKSPGKWWSTKRYILAGLAVVLAVVAVVSVFSIVLSPGEIHFSIISQASNSDPDGRHPLIELNLTLAANNTSHRTGVRYRSFLVYYLQYAASSTEKVKYSTPAKVIRAPPISQQPPLSTAEVEVSASLSMDEPAMSGGRRSNNNNIISVLLVSVVQFKAGPAYWLPYQVRVQCNPIDSLFSGSLATPINCTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6F3</accession>
    <name>I1H6F3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100821542</name>
        <name evidence="9" type="ORF">BRADI_1g65130v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PYR/PYL/RCAR abscisic acid intracellular receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558125.1">
        <property type="nucleotide sequence ID" value="XM_003558077.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6F3"/>
    <dbReference type="SMR" id="I1H6F3"/>
    <dbReference type="FunCoup" id="I1H6F3">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H6F3"/>
    <dbReference type="EnsemblPlants" id="KQK22089">
        <property type="protein sequence ID" value="KQK22089"/>
        <property type="gene ID" value="BRADI_1g65130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821542"/>
    <dbReference type="Gramene" id="KQK22089">
        <property type="protein sequence ID" value="KQK22089"/>
        <property type="gene ID" value="BRADI_1g65130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821542"/>
    <dbReference type="eggNOG" id="ENOG502QWFG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077517_1_1_1"/>
    <dbReference type="OMA" id="AHVMSPN"/>
    <dbReference type="OrthoDB" id="4436220at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07821">
        <property type="entry name" value="PYR_PYL_RCAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000021">
        <property type="entry name" value="Abscisic acid receptor PYL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF202">
        <property type="entry name" value="OS03G0297600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK22089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="227" mass="23871" checksum="0494700C8964705F" modified="2012-06-13" version="1">MPCIPASSPSSIHHHHHQRHRVLAMGCGAELAAVQGASGMARCGAHDGEVPAEVSRHHEHAAADPAGSGMRCCSAVVQHVAAPAADVWSVVRRFDQPQAYKRFVRSCALVAGDGGVGTLREVRVVSGLPAASSRERLEVLDDESHVLSFRVVGGEHRLKDYLSVTTVHPSPAAPSSATVVVESYVVDVPPGNTVEDTRVFIDTIVKCNLQSLAKTAEKLAAGGRAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1H7V0</accession>
    <name>I1H7V0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836523</name>
        <name evidence="3" type="ORF">BRADI_1g69290v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H7V0"/>
    <dbReference type="SMR" id="I1H7V0"/>
    <dbReference type="FunCoup" id="I1H7V0">
        <property type="interactions" value="1335"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77835">
        <property type="protein sequence ID" value="PNT77835"/>
        <property type="gene ID" value="BRADI_1g69290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77835">
        <property type="protein sequence ID" value="PNT77835"/>
        <property type="gene ID" value="BRADI_1g69290v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQTK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_99_3_1"/>
    <dbReference type="OMA" id="VHMDIRT"/>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056561">
        <property type="entry name" value="NFP_LYK_LysM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059143">
        <property type="entry name" value="NFP_LysM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059144">
        <property type="entry name" value="NFP_LysM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052611">
        <property type="entry name" value="Plant_RLK_LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927">
        <property type="entry name" value="LYSM-DOMAIN RECEPTOR-LIKE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927:SF2">
        <property type="entry name" value="SERINE_THREONINE RECEPTOR-LIKE KINASE NFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23446">
        <property type="entry name" value="LysM1_NFP_LYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23457">
        <property type="entry name" value="LysM2_NFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23462">
        <property type="entry name" value="LysM3_NFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="353"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT77835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="681" mass="74410" checksum="4AF832F6E06577BE" modified="2012-06-13" version="1">MKLCSAARYWLRTGAVLEIEPSPSSTSHHRCDSLRNTHTGRQMEHRRFGCPLLLLLLVVVLIFRCSEAQDVANGTERFACDVPAPCDTFVVYRTQSPGFLGLGSISDLFGVSRAMIASANNLTAKDGVLLPDQPLLVPVECGCTGNRSFANVTYPIQDGDTYYALALTAFENLTDFNIMQQLNPQAPATRLQAPEEVTVPLFCRCPTQAERAGGIRYHITYLWRPEDDMSTVSTLMNSSKSDIAEANNVTTEFTSPTAQPMLIPVSQPPKLPPLRYDASADGSGANKRGRGVAVAAGVAGSLVAFAALCVAAFAYRRYRKKKETVVQLGSPYATPKLSWHKHQQQHNYGLQSSSSLARMMNGGGDKIITSVSQFIDKPIVFGADEIMEATMNLDERCRIGSSYYRAKLDGEMFAVKPAKGDVSAEMRMTQMVNHASLIKLAGISFGTEGDYTFLVYEFAEKGSLDKWLYQKPPSSLPSSSSSSSSVDTLSWNQRLGIAFDVANGLLYMHEHTLPSMVHGDVRARNILLTADFRAKISNFSVATPAMADAADTSSDVFAFGLLILELLSGRRAMEARVGAEIGMLWRDIRAVLEAGDKRDARLRKWMDPALGSEFHMDAALSLAGMARACTEEDAARRPKMADVVFSLSMLVQPLPVGDAFEKLWQVSSEDNMGIVNEVAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H7Y9</accession>
    <name>I1H7Y9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein kinase 11-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100825962</name>
        <name evidence="6" type="ORF">BRADI_1g69590v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228955.1">
        <property type="nucleotide sequence ID" value="XM_010230653.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H7Y9"/>
    <dbReference type="FunCoup" id="I1H7Y9">
        <property type="interactions" value="2062"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H7Y9"/>
    <dbReference type="EnsemblPlants" id="KQK22836">
        <property type="protein sequence ID" value="KQK22836"/>
        <property type="gene ID" value="BRADI_1g69590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825962"/>
    <dbReference type="Gramene" id="KQK22836">
        <property type="protein sequence ID" value="KQK22836"/>
        <property type="gene ID" value="BRADI_1g69590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825962"/>
    <dbReference type="eggNOG" id="KOG1859">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010741_0_0_1"/>
    <dbReference type="OMA" id="TNISMFH"/>
    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H7Y9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000801">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000502">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF69">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 11-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="448"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="574"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK22836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1115" mass="125650" checksum="8852D03C183A85A6" modified="2012-06-13" version="1">MVTPRTGAGPPVTGDRYLDLLVRFVARHAGELLDGSVTLRLHPVGLHYVASRLEALRELEAVGAGAPVDYLRAYVADLGDHRALEQLRRILRLLTSLKVIAPGPGRDPAPLSLLPFAHLRVLELRGCDLSTSAARGLLDLRHTLEKLVCYNSTDALRHLFVSRVVDIKDSPVWSRLSYVSCASNGLVLMDESLQLLPAVETLDLSRNQFAKVDNLRKCTKLRNLDLGFNHLRSISYLSEVHSRVGKLVLRNNALTTIRGIENLKSLVGLDLSYNIISNFSELEILGSLSLLENLWLEGNPICCARWYRAHVFSFFHNSENLKLDDKGMNTQEYWEKQVMFSSRQKQPAGYGFYFPAKDDCEDEDTSKSMMVLKKISRLACIVEEERSLCDEGVDLQSTPRDSDSSKKDEVSAADGDIQITSLISTAELMKKERSTVWLREFKEWMDENTDNNTEGDRLYADFTNKNRRQKRQNGRQKSHMKRSKSVTDLAQTSEGGSSSNLLESDLSFTDGACNGVTTESPIEVNAEPDHLRVHLNSVQQLPPLEFVGTSHSDSFSELEDGSNILHANGTPSHAMSKLIGPSPSFAYPSPQSPPQYKEDILRRRLFLEEEFLQSSGDFQCVGSLGSGSSCSDDSSGDLCSCNSEDDCVAIQTKMELALNDQMASFPYADSDHEEKDGEEYLSQVGSLSDSSAEDDPTYTDAIEFGTKELHDRKQMNGHVGQHSGHLGRQNGKQKFKRRIVPIFKNQNGSKLEFLKTNGDEMDEEVSVGENGHLRYDLKSTTCKDQHSEKHISSILHKDNLSIGADTVSSNTRRGKYKLIEDFFNVEVANKEESEICEQGARCGYMFQDGSDLVQREVALLRSSQNKLYVLLVDMAQDEREIMPRVLGCYRLEHLEKVLIGLGLQVLRMHMTDHTAHLFLARTPKEAQDVLWLLNLFSFPQLTNGVSLQSWEKIQIKSLEKCICASMKMGIFFYSMLMFWKNDAEEECLVIRSLIVTEGSIFVCIEDLYRFGCFPDDSYPPYFSLDEYCSIKNIKELVMDQRDNKCLTLVLDKHIHEGRSHSTTKNSQNKQADAIYKIQSWKLKWFAEETLMKFISVLRALYSTAGSSSLPVKWIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCG7</accession>
    <name>I1HCG7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100841117</name>
        <name evidence="6" type="ORF">BRADI_2g04497v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565398.1">
        <property type="nucleotide sequence ID" value="XM_003565350.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCG7"/>
    <dbReference type="STRING" id="15368.I1HCG7"/>
    <dbReference type="EnsemblPlants" id="KQK02932">
        <property type="protein sequence ID" value="KQK02932"/>
        <property type="gene ID" value="BRADI_2g04497v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841117"/>
    <dbReference type="Gramene" id="KQK02932">
        <property type="protein sequence ID" value="KQK02932"/>
        <property type="gene ID" value="BRADI_2g04497v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841117"/>
    <dbReference type="eggNOG" id="KOG0406">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_18_0_1"/>
    <dbReference type="OMA" id="WIPVYNG"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000355">
        <property type="entry name" value="Glutathione S-transferase U10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase U9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF232">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="98"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK02932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="254" mass="27496" checksum="A8E519C6C3C32F2C" modified="2012-06-13" version="1">MAAAAAEEGRLKLLGFWSSPFVLRTRYALNLKGIPYTNVEEDLFGAKSELLLAANPAHGGKVPALLLPDGRSVSESLIIVEYLDEAFPGTPPRLLPSDPHRRATARFWAAHVDGALLPAWIPLYGGSTAEERVEAARRVVGVLETFEGVLRRGDCCSSGEEEEGGFGFFGGDSVGLVDVVLGGFIGWLRASEAMCGVKTIEDGSRTPLLAAWAERFAALEGVREILPEVGRLVEYNQMKRALRGLPFLPPHQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HEQ7</accession>
    <name>I1HEQ7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="18">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC100824801</name>
        <name evidence="23" type="ORF">BRADI_2g11250v3</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">Bd21</strain>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="18">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="18">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="18">Binds 2 divalent ions per subunit. The metal ions interact primarily with the substrate. Can utilize magnesium, manganese or cobalt (in vitro).</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 18">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 19">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="18"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567567.1">
        <property type="nucleotide sequence ID" value="XM_003567519.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HEQ7"/>
    <dbReference type="SMR" id="I1HEQ7"/>
    <dbReference type="STRING" id="15368.I1HEQ7"/>
    <dbReference type="EnsemblPlants" id="KQK04025">
        <property type="protein sequence ID" value="KQK04025"/>
        <property type="gene ID" value="BRADI_2g11250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824801"/>
    <dbReference type="Gramene" id="KQK04025">
        <property type="protein sequence ID" value="KQK04025"/>
        <property type="gene ID" value="BRADI_2g11250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824801"/>
    <dbReference type="eggNOG" id="KOG1506">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041802_0_1_1"/>
    <dbReference type="OMA" id="CEYAFRH"/>
    <dbReference type="OrthoDB" id="5852090at2759"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000001">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 18" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="15 18" id="KW-0630">Potassium</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="20">
        <location>
            <begin position="7"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="21">
        <location>
            <begin position="120"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="22">
        <location>
            <begin position="243"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KQK04025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="396" mass="43236" checksum="AA8B8C5FD8D4C943" modified="2012-06-13" version="1">MAEIDTFLFTSESVNEGHPDKLCDQISDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRGIGFVSNDVGLDADHCKVLVNIEQQSPDIAQGVHGNFTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYHNDNGAMVPIRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEQYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYVARQAAKSIVASGLARRCIVQVSYAIGVPDPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGMIIINLDLKRGGNDRYLKTAAYGHFGRDGPDFTWEVVKPLEWKKPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HHK1</accession>
    <name>I1HHK1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Globin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100828385</name>
        <name evidence="8" type="ORF">BRADI_2g19690v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Fe(III)-heme b-[protein] + nitric oxide + H2O = Fe(II)-heme b-[protein] + nitrite + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:77711"/>
            <dbReference type="Rhea" id="RHEA-COMP:18975"/>
            <dbReference type="Rhea" id="RHEA-COMP:18976"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:77713"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant globin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568090.1">
        <property type="nucleotide sequence ID" value="XM_003568042.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HHK1"/>
    <dbReference type="FunCoup" id="I1HHK1">
        <property type="interactions" value="863"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HHK1"/>
    <dbReference type="EnsemblPlants" id="KQK05367">
        <property type="protein sequence ID" value="KQK05367"/>
        <property type="gene ID" value="BRADI_2g19690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828385"/>
    <dbReference type="Gramene" id="KQK05367">
        <property type="protein sequence ID" value="KQK05367"/>
        <property type="gene ID" value="BRADI_2g19690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828385"/>
    <dbReference type="eggNOG" id="KOG3378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_11_2_1"/>
    <dbReference type="OMA" id="GAKQMFP"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001032">
        <property type="entry name" value="Leghaemoglobin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22924">
        <property type="entry name" value="LEGHEMOGLOBIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22924:SF95">
        <property type="entry name" value="OS05G0517600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00188">
        <property type="entry name" value="PLANTGLOBIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="7">
        <location>
            <begin position="2"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK05367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="159" mass="17440" checksum="481E7CF80983A1A1" modified="2012-06-13" version="1">MGFSEAQEELVLRSWKAMKPDSESIALKFFLRIFEIAPAAKPMFPFLREAGGDDVPLETHPKLKAHAVTVFVMACESATQLRKTGDVKVREASLRRLGATHVKAGVADAHFEVVKTALLETIEGAVPGMWTPEMKGAWEEAYDQLAAAIKDEMKLAAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1HHV5</accession>
    <name>I1HHV5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calreticulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100838375</name>
        <name evidence="16" type="ORF">BRADI_2g20510v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 14">Belongs to the calreticulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566063.1">
        <property type="nucleotide sequence ID" value="XM_003566015.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HHV5"/>
    <dbReference type="STRING" id="15368.I1HHV5"/>
    <dbReference type="EnsemblPlants" id="KQK05518">
        <property type="protein sequence ID" value="KQK05518"/>
        <property type="gene ID" value="BRADI_2g20510v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838375"/>
    <dbReference type="Gramene" id="KQK05518">
        <property type="protein sequence ID" value="KQK05518"/>
        <property type="gene ID" value="BRADI_2g20510v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838375"/>
    <dbReference type="eggNOG" id="KOG0674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="NQWTISE"/>
    <dbReference type="OrthoDB" id="1938156at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HHV5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.250.10:FF:000002">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000018">
        <property type="entry name" value="Calreticulin 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009169">
        <property type="entry name" value="Calreticulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF28">
        <property type="entry name" value="CALRETICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002356">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00803">
        <property type="entry name" value="CALRETICULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 11 14" id="KW-0143">Chaperone</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 11 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982674" description="Calreticulin" evidence="14">
        <location>
            <begin position="25"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="216"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="363"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="363"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR002356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR002356-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR002356-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK05518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="422" mass="49691" checksum="FBAD4E39C7E77A88" modified="2012-06-13" version="1">MMGCLYRVLHGVLALSSLLVLASGEVFFEERFEDGWGSRWVKSDWKKSEGKAGAFRHTAGSFSGDPDDKGIQTTLDARHFAISAKYPEFSNKNRTLVVQYSIKIEQDIECGGAYIKLMSGYVNQKKFGGDTPYSFMFGPDICGDQTKKLHLILSYQGQNYPIKKDLKCETDKLTHFYTFILRPDASYSLLIDNREREFGSMYTDWDILPPRKIKQVNAKKPKDWDDREYIEDPDEVKPEGYDSIPKEIPDPNDKKPESWDDDEDGIWKPRMIPNPEYKGPWKHKKIKNPNYKGKWKAPWIDNPEFEDDPDLYVLKPLQYVGIEVWQVKAGSVFDNILICDDPEYARKVVDEIWAANKEVEKEAFEGAEKKRKAREDEEARRAREEGEKRRRERDRHRGRDHYREKHKRRYRRDYMDDYHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1HN33</accession>
    <name>I1HN33_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100830206</name>
        <name evidence="12" type="ORF">BRADI_2g39517v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569114.1">
        <property type="nucleotide sequence ID" value="XM_003569066.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HN33"/>
    <dbReference type="STRING" id="15368.I1HN33"/>
    <dbReference type="EnsemblPlants" id="PNT72119">
        <property type="protein sequence ID" value="PNT72119"/>
        <property type="gene ID" value="BRADI_2g39517v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830206"/>
    <dbReference type="Gramene" id="PNT72119">
        <property type="protein sequence ID" value="PNT72119"/>
        <property type="gene ID" value="BRADI_2g39517v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830206"/>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000837_14_1_1"/>
    <dbReference type="OMA" id="THDERDN"/>
    <dbReference type="OrthoDB" id="590607at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1114">
        <property type="entry name" value="OS02G0475500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="17"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="176"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="417"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="679"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="802"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="970"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="353"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT72119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1220" mass="137731" checksum="22E95B92CC7CA106" modified="2012-06-13" version="1">MEATALSVGKSVLDGAIGYAKSAFAEEVALQLGVRRDQVFITNELEMMKAFLESAHGEGADDKVVKVWVKQVRDMAYDVEDCLQEFAVRLNKQSWWRIRRKMLDRRRVAKQMKELRANVEDVSQRNTRYSLIKGSGSKAATYTAAEQSSITSAALFGIDEARRAAKQEKSQVDLVQMINEEENNLRVIAVWGTSGGLGQTSIVRKAYENPDIESKFSCRAWVRLMHPFDPKDFVQSMVEQFNAAGGVNVLLRKKKTVQELTNKFEQYVNEKRYLIVINDLSTIEEWDRVKKCFPNNNMGSRIIVSTTQVEVASLCAGQGSVASELKQFSAGQNIYAFHDKGSQDATDLINEGSSSNTASTSTANKSTVPTCEISEENTVKKSLTRIRTMVAALEESHLVGREKEKSEIIKLVTGQATGQFEVITVWGMGGLGKTTLVKDIYQSQELSDIFDNRACVTVMRPFNLEELLRSLFMQLDRESSEKKDVVGLMSSTKNTLLLMSLAELIKELAKLFQTKRCLIVLDDVLSTAEWDRIEPIFHEMRKTIRIIVTTREESIAKHCCSQKQENIYKLKDLEYKDAHDLFTKKVFKGTVDLDKQYPELVQPAKLILKKCDQLPLAIVTIACFLATQPKAPLEWRKLSEHLSAELEMNPQLGTIRTVLMRSYDGLPYHLKSCFLYMPIFPEDHRVGRGRLARRWSAEGYCREVRGKSAEEMADSYFMELIARSMILPPQESIRCTKGIDSCQVHDLMREIGISKSMEENLVFTLEQGCSTNSQVTMRHLAISSNWEGDQNEFESMVDVSRVRSLTVFGKWKSFFISDRMRLLRVLDLEDATGLLDHHLKHIGKFLHLRYLSLRGCGGIFHLPDLLGNLRQLETLDIRGTNVVMLPKTIIKLLKLKSLRAGEVLLDEDVSYEEIIDDFPKVMRTRLCILPIFSLECYLFWCAPRRMDADMTRHDVCAFFCCVILPVVAMHLDRYGVLVPRGMKKLKSLHTLGVVNIGRRGKAVLKDIKGLIQLRKLGVTGVNKENGQELCSAILGLSRLESLSIRSEGRPGLCGCLDGTFSFPENLQSLKLYGNLVKLPEWVQGLKNLVKLKLRSSSISEPDAAIQVLGILPNLAFLHLLGKSFVGEEVRLNFPREMFPSMIVLELELEVVLRAVKFEQGATPRLELLKFCNVRINSDSLSGLASLSGLKEIVTSTYLEDVRAAVAENLNRPLVRSWSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPD0</accession>
    <name>I1HPD0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cysteine proteinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100841730</name>
        <name evidence="7" type="ORF">BRADI_2g43410v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566879.1">
        <property type="nucleotide sequence ID" value="XM_003566831.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPD0"/>
    <dbReference type="STRING" id="15368.I1HPD0"/>
    <dbReference type="EnsemblPlants" id="KQK08706">
        <property type="protein sequence ID" value="KQK08706"/>
        <property type="gene ID" value="BRADI_2g43410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841730"/>
    <dbReference type="Gramene" id="KQK08706">
        <property type="protein sequence ID" value="KQK08706"/>
        <property type="gene ID" value="BRADI_2g43410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841730"/>
    <dbReference type="eggNOG" id="KOG1543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012184_1_0_1"/>
    <dbReference type="OMA" id="PRRGHSM"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000142">
        <property type="entry name" value="Cysteine proteinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982778" description="Cysteine proteinase" evidence="4">
        <location>
            <begin position="30"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="6">
        <location>
            <begin position="38"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="5">
        <location>
            <begin position="133"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK08706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="37459" checksum="61069C3BAD4AE833" modified="2012-06-13" version="1">MASIPSSRRLIICCALMLVLAAAASAGEARHVTVASRHERWMARFGRAYKDADEKARRQEVFGANARHVDAVNRSGNRTYTLGLNHFSDLTDHEFLQQHLGYRHHQPGPGGLLRPEDQDMSKATALADYGQDVPDSVDWRAQGAVTEIKNQRSCGSCWAFAAVAATEGLVKIATGNLISMSEQQVLDCTGGGNTCDGGDINAALRYVAASGGLQPEAAYAYAAQKGACRGASPANSAASVGGARFARLGGDEGALRGLAAGQPVAVALEASEPDFRHYKSGVYAGSASCGRRLNHGVTVVGYGAEDDSGDEYWVVKNQWGTLWGEKGYMRVARGDVAGANCGIASYAYYPTMDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1HR87</accession>
    <name>I1HR87_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription repressor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ovate family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100839306</name>
        <name evidence="9" type="ORF">BRADI_2g48970v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional repressor that regulates multiple aspects of plant growth and development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569734.1">
        <property type="nucleotide sequence ID" value="XM_003569686.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HR87"/>
    <dbReference type="STRING" id="15368.I1HR87"/>
    <dbReference type="EnsemblPlants" id="KQK09595">
        <property type="protein sequence ID" value="KQK09595"/>
        <property type="gene ID" value="BRADI_2g48970v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839306"/>
    <dbReference type="Gramene" id="KQK09595">
        <property type="protein sequence ID" value="KQK09595"/>
        <property type="gene ID" value="BRADI_2g48970v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839306"/>
    <dbReference type="eggNOG" id="ENOG502QSY1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072908_0_0_1"/>
    <dbReference type="OMA" id="FGASEWE"/>
    <dbReference type="OrthoDB" id="679311at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038933">
        <property type="entry name" value="Ovate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006458">
        <property type="entry name" value="Ovate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01568">
        <property type="entry name" value="A_thal_3678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057:SF3">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR OFP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04844">
        <property type="entry name" value="Ovate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51754">
        <property type="entry name" value="OVATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OVATE" evidence="8">
        <location>
            <begin position="261"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="24"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="121"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="191"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="220"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK09595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="330" mass="35798" checksum="111A61DA07D03AB2" modified="2012-06-13" version="1">MEGSGSGTGRKKLKHRLAAILSVFSRRSGSSRKRRDEEAPPPPLALPSYARLAGGGKKAAGWGGAQHDRRLSVSERRTVPLIRITIDCAGRRSVDAADPTLLAPLDADNYARKKLGLPYETSDWDGRKCPPSSPFPVAPHLPPMPPRWKERPSSRGRPSTHSSRRLLSSSSSDDEYDEESSSLRNIFSSRSFSSDSSDFYNCPRNSKNTSTTKPRASVSGPCRAPPAAGGARRGASQSCRYSFELPRGSTASAATDGGFAVVKRSADPYEDFRKSMEEMIAEWPAGGHGHGEEDEHSAEGLLETYLVLNSPRHYPAILAAFADVRETLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HVE4</accession>
    <name>I1HVE4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NAD(P)H-quinone oxidoreductase subunit O, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100820990</name>
        <name evidence="7" type="ORF">BRADI_2g61410v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565117.1">
        <property type="nucleotide sequence ID" value="XM_003565069.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HVE4"/>
    <dbReference type="STRING" id="15368.I1HVE4"/>
    <dbReference type="EnsemblPlants" id="KQK11649">
        <property type="protein sequence ID" value="KQK11649"/>
        <property type="gene ID" value="BRADI_2g61410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100820990"/>
    <dbReference type="Gramene" id="KQK11649">
        <property type="protein sequence ID" value="KQK11649"/>
        <property type="gene ID" value="BRADI_2g61410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100820990"/>
    <dbReference type="eggNOG" id="ENOG502RZEJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130659_1_0_1"/>
    <dbReference type="OMA" id="MINETYW"/>
    <dbReference type="OrthoDB" id="2014260at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020905">
        <property type="entry name" value="NdhO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36728">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT O, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36728:SF2">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT O, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11910">
        <property type="entry name" value="NdhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0618">Plastoquinone</keyword>
    <keyword evidence="1" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK11649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17375" checksum="4A059E4FF56889AB" modified="2012-06-13" version="1">MEALASSSPRLALFSRTATAANWASSSSLVRRSPAARVKAAAEPAGEAKPAAAAAATADGAPAPKKILKKKPVYSMKKGQIVRVEKEKYLNSINYLSVGHPPFYKGLDYIYEDRGEVLDIRVFETGEYALIAWVGIPTPPAWLPTYMLIKSDKLDYERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYI4</accession>
    <name>I1HYI4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor HY5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100839215</name>
        <name evidence="13" type="ORF">BRADI_3g07540v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571497.1">
        <property type="nucleotide sequence ID" value="XM_003571449.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYI4"/>
    <dbReference type="STRING" id="15368.I1HYI4"/>
    <dbReference type="EnsemblPlants" id="KQJ93926">
        <property type="protein sequence ID" value="KQJ93926"/>
        <property type="gene ID" value="BRADI_3g07540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839215"/>
    <dbReference type="Gramene" id="KQJ93926">
        <property type="protein sequence ID" value="KQJ93926"/>
        <property type="gene ID" value="BRADI_3g07540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839215"/>
    <dbReference type="eggNOG" id="KOG1414">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068771_1_0_1"/>
    <dbReference type="OMA" id="SAHHMEM"/>
    <dbReference type="OrthoDB" id="674948at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010017">
        <property type="term" value="P:red or far-red light signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009585">
        <property type="term" value="P:red, far-red light phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010099">
        <property type="term" value="P:regulation of photomorphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010218">
        <property type="term" value="P:response to far red light"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14704">
        <property type="entry name" value="bZIP_HY5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.490:FF:000004">
        <property type="entry name" value="Transcription factor HY5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044280">
        <property type="entry name" value="Hac1/HY5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714:SF6">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0607">Phytochrome signaling pathway</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BZIP" evidence="12">
        <location>
            <begin position="82"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="118"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00084091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ93926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="156" mass="17231" checksum="C3711F56D9712F07" modified="2012-06-13" version="1">MQEQGESSRPSSSERSSSSGGHHMEPKEGMESDDEIGMVPELGLEPGGPSTSGRAGGPERAGQSSTAQGSARRRGRTPADKEHKRLKRLLRNRVSAQQARERKKAYLGDLEVKVKDLEKKNSELEERHSTLQNENQMLRQILKNTTVSRRGPSEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1D5</accession>
    <name>I1I1D5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100821114</name>
        <name evidence="10" type="ORF">BRADI_3g16340v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571453.1">
        <property type="nucleotide sequence ID" value="XM_003571405.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1D5"/>
    <dbReference type="FunCoup" id="I1I1D5">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I1D5"/>
    <dbReference type="EnsemblPlants" id="KQJ95298">
        <property type="protein sequence ID" value="KQJ95298"/>
        <property type="gene ID" value="BRADI_3g16340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821114"/>
    <dbReference type="Gramene" id="KQJ95298">
        <property type="protein sequence ID" value="KQJ95298"/>
        <property type="gene ID" value="BRADI_3g16340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821114"/>
    <dbReference type="eggNOG" id="ENOG502QRMZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047766_0_0_1"/>
    <dbReference type="OMA" id="GACYCEP"/>
    <dbReference type="OrthoDB" id="551907at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010158">
        <property type="term" value="P:abaxial cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044847">
        <property type="entry name" value="KAN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31496:SF59">
        <property type="entry name" value="OS08G0160300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31496">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="146"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ95298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="329" mass="35162" checksum="0E253C0C36D53984" modified="2012-06-13" version="1">MELFPSQPDLSLQIGLPATATRHDHHHQARFFGPPSSNNPAMAPPPPLQLPFPMPHLPMPMPPGMGHGGGLYYPNGGLLRPIRGVPLYGPGHGPGPHVVANNPAMAPPCYCDPYARRSGGACGGGGGGGRILGGFPAPKRAARAPRMRWTSTLHSRFVHAVELLGGHDRATPKAVLELMDVKDLTLAHVKSHLQMYRTVKNTERPAASSDQADGFENGSAGEICDDNLLDLHGVGGRTEAARHARLAAANDHDGSISAGALWNSSSREEDWSGFPCDSNNESMQSFKDHMQSKSLEILSDINSCLSETTSSASEPNLDFTLGRPHHRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2A4</accession>
    <name>I1I2A4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100825733</name>
        <name evidence="2" type="ORF">BRADI_3g18920v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573600.1">
        <property type="nucleotide sequence ID" value="XM_003573552.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2A4"/>
    <dbReference type="STRING" id="15368.I1I2A4"/>
    <dbReference type="EnsemblPlants" id="KQJ95771">
        <property type="protein sequence ID" value="KQJ95771"/>
        <property type="gene ID" value="BRADI_3g18920v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825733"/>
    <dbReference type="Gramene" id="KQJ95771">
        <property type="protein sequence ID" value="KQJ95771"/>
        <property type="gene ID" value="BRADI_3g18920v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825733"/>
    <dbReference type="eggNOG" id="KOG2398">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027682_0_0_1"/>
    <dbReference type="OMA" id="QPTNGPD"/>
    <dbReference type="OrthoDB" id="20621at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018808">
        <property type="entry name" value="Muniscin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37769">
        <property type="entry name" value="OS08G0243900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37769:SF1">
        <property type="entry name" value="OS08G0243900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10291">
        <property type="entry name" value="muHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD" evidence="1">
        <location>
            <begin position="395"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ95771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="633" mass="67366" checksum="A14525579CC821F8" modified="2012-06-13" version="1">MACLAISLQPVNGPDILLQTRSWFPVSRALAAVSAFRLARLHLARGKQQPASHASLDAIGDDPLAAGSGQLVVGVESQYRVVYRLVNSIYVLGVTTASDHASPAVHAFAVADAVNQAVSVVVAACRGVDVTPEKVHRKYPEVYLALDLVIHGVGSVRLSQILATIHGDNLARMVNSSPDAEARARGADSWPTVEHLAQDRHAARDAFTGASFELPLETLAAGDEFSASSLAPAAAATTGDEAPPEDAPPVEKDPFAASDMVSKPEEALVGGFKKNKETALVVADPAAALAGLEVTTLPPAEATKPTFIGVEGFEGDYGGIEFGNEEASLAEAFEGFNAPFGGGLDASEFVTTTKKDHKDKSITGLELLAMGGGQALNAAAGSPLENLLVTKSAEMTVPELYIVEEINAEFKESILARVGLKGTIFLRTLPPKKAAGRDTEFSFRLEGTSGMKRAALQSTVLSNLENGLFHVKTPSKEEPIPIMKYSFLPKHSPLPLRMRLVKRHSGTLLSVMIHYASNPILPQPLSNVTFIVKLPVDPTLLNVSPKAVLNRAERELRWHIADIPLKGPAGRLRARMPVDQDSKDGELEVVGMVKFTYQGPFTLSGIKLCPAINSTAQFNQVGHTFSSGSYRCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2I6</accession>
    <name>I1I2I6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100839421</name>
        <name evidence="10" type="ORF">BRADI_3g19620v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573643.1">
        <property type="nucleotide sequence ID" value="XM_003573595.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2I6"/>
    <dbReference type="EnsemblPlants" id="PNT67012">
        <property type="protein sequence ID" value="PNT67012"/>
        <property type="gene ID" value="BRADI_3g19620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839421"/>
    <dbReference type="Gramene" id="PNT67012">
        <property type="protein sequence ID" value="PNT67012"/>
        <property type="gene ID" value="BRADI_3g19620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839421"/>
    <dbReference type="eggNOG" id="KOG1278">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010714_4_1_1"/>
    <dbReference type="OMA" id="TILITYH"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF154">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007362396" description="Transmembrane 9 superfamily member" evidence="9">
        <location>
            <begin position="27"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="343"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="492"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="531"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="571"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="602"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT67012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="641" mass="73282" checksum="23A372221ECE568E" modified="2012-06-13" version="1">MAISPAEASALAVALVVLCLAAPAAGFYLPGVAPNDFDQKDLLPVKVNKLTSIKTQLPYSFYSLPFCKPDTIVDSAENLGEVLRGDRIENSPYVFEMREPQMCQIVCKISVGEKEAKVLKEKIEDEYRVNMILDNLPLVVPIQRVDQEGAYFYQHGFHVGAKGQYSGSKDEKYFIHNHLSFTVKYHRDAQRDVSRIVAFEVKPYSVKHEYEGQWNDKKTRLTTCDPHAKRIITSSDSPQEVEAGKDIVFTYDVDFKESDIKWASRWDSYLLMTDDQIHWFSIVNSLMIVLFLSGMVAMIMLRTLYRDISKYNQLETQEEAQEETGWKLVHGDVFRPPANSDWLCVYVGTGVQFFGMMLVTMVFAVLGFLSPSNRGGLMTAMLLLWVFMGLLAGYSSSRLYKLFKGSEWKNIALRTAFTFPGSVFAIFFFLNILIWGQKSSGAVPFTTMFALVLLWFGISVPLVFVGSFLGFKKPAIEDPVKTNKIPRQVPEQAWYMNPIFSILIGGILPFGAVFIELFFILTSIWLHQFYYIFGFLFLVFLILIVTCAEISIVLCYFQLCSEDYLWWWRSYLTSGSSALYLFLYATFYFFTKLEITKFVSAVLYFGYMLIASYAFFALTGTIGFYACFMFTRLIYSSVKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1I3K0</accession>
    <name>I1I3K0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100823792</name>
        <name evidence="8" type="ORF">BRADI_3g23066v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014755747.1">
        <property type="nucleotide sequence ID" value="XM_014900261.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I3K0"/>
    <dbReference type="FunCoup" id="I1I3K0">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I3K0"/>
    <dbReference type="EnsemblPlants" id="KQJ96433">
        <property type="protein sequence ID" value="KQJ96433"/>
        <property type="gene ID" value="BRADI_3g23066v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823792"/>
    <dbReference type="Gramene" id="KQJ96433">
        <property type="protein sequence ID" value="KQJ96433"/>
        <property type="gene ID" value="BRADI_3g23066v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823792"/>
    <dbReference type="eggNOG" id="ENOG502QQ3S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025359_1_1_1"/>
    <dbReference type="OMA" id="HGNQVDQ"/>
    <dbReference type="OrthoDB" id="1728340at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I3K0">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="15"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="187"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ96433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="366" mass="40487" checksum="454D6A89C0150ED8" modified="2012-06-13" version="1">MELAGRWRKLMPYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNAVAAVIMAPFALWFERKTRPKMTLSLFYKILALGLLEPVLDQNFFYMGAKNTSATFSSALTNVLPAVTFVNAILLRMERISINERRSQAKIAGTMITVAGAAVMILFSGPVVNFPWSRHGSSHSAVADGTSHSSGRMLMGIFMILFSCFCWSLFFILQSYTLRSYPSELSLTTLICAMGVAQSGALALVMERDIKAWAIGFDMRLFTAVYSGIMCSGVAYYVQGIVIQERGPVFVTAFSPLCMIIVTVLGSFILSEVITLGRLIGAIIIVIGLYALIWGKSNDHVVQVVREDSFDKHKVFELPFTATNITKASNLEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1I7E6</accession>
    <name>I1I7E6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100833415</name>
        <name evidence="16" type="ORF">BRADI_3g36912v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574538.1">
        <property type="nucleotide sequence ID" value="XM_003574490.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I7E6"/>
    <dbReference type="STRING" id="15368.I1I7E6"/>
    <dbReference type="EnsemblPlants" id="PNT68191">
        <property type="protein sequence ID" value="PNT68191"/>
        <property type="gene ID" value="BRADI_3g36912v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833415"/>
    <dbReference type="Gramene" id="PNT68191">
        <property type="protein sequence ID" value="PNT68191"/>
        <property type="gene ID" value="BRADI_3g36912v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833415"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_59_0_1"/>
    <dbReference type="OMA" id="TPELCGH"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000279">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000096">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF149">
        <property type="entry name" value="CBL-INTERACTING PROTEIN KINASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="21"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="NAF" evidence="15">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="PNT68191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="452" mass="49087" checksum="CCE7149FAD043D34" modified="2012-06-13" version="1">MAEAAAAEGKKGGGGVLQGRYEMGRVLGHGNFGRVHVARDLRTGRSVAVKVVAKEKVVRAGMAEQMKREIAVMKRVSHPNIVELHEVMATRSKIYLALELVRGGELFARIVRSGRVREDVARHYFRQLISAVDFCHARGVFHRDLKPENLLLDEQGNLKVVDFGLSALADHARADGLLHTLCGTPGYAAPEVLRDKGYDGAKADIWSCGVILFVLLAGSLPFPDDNIVTMYKKVQRGDYRCPPWFSTDARRLVPKLLDPNPATRITIAELVETPWFKKTSIARPVTTEPPPPPIDHPVVCAAKEAEDKDEPETMNAFHLISLSEGFDLSPLFEGNSARGRREGGMLFATREPAKGVVSRLEEVAARGGGRMRVTKSGAGGVRFEGAARGGAKGRLAVAAEIFSVAPSVLVVDVKKDGGDTLEYRSFCSEELRPALQDIVWAADQPASVALAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1IAH6</accession>
    <name>I1IAH6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100838406</name>
        <name evidence="5" type="ORF">BRADI_3g45740v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575134.1">
        <property type="nucleotide sequence ID" value="XM_003575086.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IAH6"/>
    <dbReference type="SMR" id="I1IAH6"/>
    <dbReference type="FunCoup" id="I1IAH6">
        <property type="interactions" value="2536"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IAH6"/>
    <dbReference type="EnsemblPlants" id="KQJ99872">
        <property type="protein sequence ID" value="KQJ99872"/>
        <property type="gene ID" value="BRADI_3g45740v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68827">
        <property type="protein sequence ID" value="PNT68827"/>
        <property type="gene ID" value="BRADI_3g45740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838406"/>
    <dbReference type="Gramene" id="KQJ99872">
        <property type="protein sequence ID" value="KQJ99872"/>
        <property type="gene ID" value="BRADI_3g45740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68827">
        <property type="protein sequence ID" value="PNT68827"/>
        <property type="gene ID" value="BRADI_3g45740v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838406"/>
    <dbReference type="eggNOG" id="KOG1426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005210_1_1_1"/>
    <dbReference type="OMA" id="TGAAKCW"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IAH6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF371">
        <property type="entry name" value="OS02G0554100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="17"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="39"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="93"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="145"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="197"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="249"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="301"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="353"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ99872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="448" mass="48017" checksum="6E9203BC178BE5DD" modified="2012-06-13" version="1">MDAKAEAAAGDEEVPEAPEQTVALVSAGANHSVALLSSKAVCSWGRGEDGQLGHGDAEDRPVPTLLSGFDVPGIASVVICGADHTTAYSDEQMQLYSWGWGDFGRLGHGNSSDVFHPQPIEALQGIKIKQIACGDSHCLAVTDAGQVHSWGRNQNGQLGLGNTEDSLLPQKIHAFEGVCVKMIAAGAEHTAAILEDGDLYGWGWGQYGNLGLGDRNDRLVPEKVSSVEGEKMVLVACGWRHTVTVSSSGDLYTYGWSKYGQLGHGDFEDHLVPHKLEALKDRFISQIAGGWRHTMAVAGDGKLYGWGWNKFGQVGVADNVDHCSPVQVIFPDEQKVAQVACGWRHTLALTENKNVFSWGRGTSGQLGHGEIIDRNTPKMIDALSLDGYACKNLESSKAVPMIAEVRITPSARYALVPDENVCKPGEGILQGNGTDTHVPQSDAKRMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBN4</accession>
    <name>I1IBN4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100833300</name>
        <name evidence="4" type="ORF">BRADI_3g48960v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575376.1">
        <property type="nucleotide sequence ID" value="XM_003575328.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBN4"/>
    <dbReference type="STRING" id="15368.I1IBN4"/>
    <dbReference type="EnsemblPlants" id="KQK00375">
        <property type="protein sequence ID" value="KQK00375"/>
        <property type="gene ID" value="BRADI_3g48960v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69083">
        <property type="protein sequence ID" value="PNT69083"/>
        <property type="gene ID" value="BRADI_3g48960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833300"/>
    <dbReference type="Gramene" id="KQK00375">
        <property type="protein sequence ID" value="KQK00375"/>
        <property type="gene ID" value="BRADI_3g48960v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69083">
        <property type="protein sequence ID" value="PNT69083"/>
        <property type="gene ID" value="BRADI_3g48960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833300"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_0_2_1"/>
    <dbReference type="OMA" id="NHFIYPH"/>
    <dbReference type="OrthoDB" id="597215at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016556">
        <property type="term" value="P:mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002973">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000277">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF453">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT (PPR) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="177"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK00375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="58044" checksum="0D32EFEF9B8AA1CB" modified="2012-06-13" version="1">MLPAPPFSHGDFVAVLSRCATLAHLKQLHGRSVVTARAASQSTTFQLLRFASLRLSCLPYARRLFDSTPHPNVFLYSAMLSAYAAASPAQAYGHDALALFLRMLRRGRPAPNQFVYPLVLRAACAIGVQLVRSIHSHACKSGFHAYDVIRTSLLDGYSRYGMMLDARKLFDGLTERNVISWTALVSGYARAGKIGDAIVLFDRMPERDVAAWNAMITGCTQNGLFVEAVGICSRMVDEGFQLNGTTVSCVLSACGHLGMLKIGKVVHGYAWRSCVGFGSSVVNGLIDMYGKCGNLKEARWMFDEFSDRSLTTWNSLINCLALHGHSKSAIAVFDEMRDEGIEPDVVTFVGLLNACTHGGFVDEGLTYFKLMCDELRIEPEIEHYGCIVDLLGRAGRFEDAMNVINDMKVQPDEVIWGSLLNACRTHRQLELAEFSVRKLLQLNPNNANYVVMLANLYSEGGSWEEVTKIRKLMKEDIMGKKLPGCSWIEVDRKTHRFYSGDDAHPESEDIYDTLDELAASMEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICL1</accession>
    <name>I1ICL1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100823997</name>
        <name evidence="9" type="ORF">BRADI_3g51660v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235817.1">
        <property type="nucleotide sequence ID" value="XM_010237515.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235818.1">
        <property type="nucleotide sequence ID" value="XM_010237516.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICL1"/>
    <dbReference type="STRING" id="15368.I1ICL1"/>
    <dbReference type="EnsemblPlants" id="KQK00764">
        <property type="protein sequence ID" value="KQK00764"/>
        <property type="gene ID" value="BRADI_3g51660v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69234">
        <property type="protein sequence ID" value="PNT69234"/>
        <property type="gene ID" value="BRADI_3g51660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823997"/>
    <dbReference type="Gramene" id="KQK00764">
        <property type="protein sequence ID" value="KQK00764"/>
        <property type="gene ID" value="BRADI_3g51660v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69234">
        <property type="protein sequence ID" value="PNT69234"/>
        <property type="gene ID" value="BRADI_3g51660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823997"/>
    <dbReference type="eggNOG" id="KOG1161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025236_1_0_1"/>
    <dbReference type="OMA" id="RANHPCS"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ICL1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14479">
        <property type="entry name" value="SPX-MFS_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051068">
        <property type="entry name" value="MFS_Domain-Containing_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045264">
        <property type="entry name" value="SPXM_SPX_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YAJR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510:SF23">
        <property type="entry name" value="SPX DOMAIN-CONTAINING MEMBRANE PROTEIN OS02G45520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="512"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="575"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="605"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="469"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK00764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="696" mass="77343" checksum="7FC7B12A712700AB" modified="2012-06-13" version="1">MVNFGKALMADQFEEWKEYYINYKMMKKKVKQYVQQTQNGGRNHEQVLKEFSRMLDDQIETVVLFLLKEQGHLASRIEKLGQQRAILSDQVDVSQVSQLREAYREVGLDLVKLLRFVDMNATGIRKILKKFDKRFGYRFTDYYVSTRANHPYSQLQPIFKQVGIVAVAGALTRNLATLQDHHGSFTSIYDHPSITLKDPVIEQINHSVQKLTHSTTFLKFLGQHALIVPEDMHSSSDDLVDDQSYHFMSLLLNLVNTFLYMVNTYIIVPTADDYSVSLGAAATVCGVIIGSMAVAQVFSSVYFSAWSNKSYFRPLVFSSIMLFSGNLLYALAYDVNSLTVLILGRLLCGLGSARAVNRRYISDCVPLKIRLKASAGFVSASALGMACGPAFAGLLQTEFKIYGVTFNQNTLPGWVMCVAWVANLFWLWISFKEPDHIAKANAANTQSSDSGHGRSDNLEDGLAQPLLTEAKERQDENVEDNDEKEDPKESHKPATSLAAAYRLLTPSVKVQLLIYFMLKFVMEILLSESSVVTSFYFQWSTSSVAIFLAVLGLTVLPVNVIVGSYITNLFQDRQILVASEIMVLIGIATSFHFTSHYSVPQYVSSALITFVFAEVLEGVNLSLLSRVMSSRLSRGTYNGGLLSTEAGTLARVAADVTITAAGYLGQSCLLNITLLPSLAICVTSIAATFCTYNSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICY0</accession>
    <name>I1ICY0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3-dehydrosphinganine reductase</fullName>
            <ecNumber evidence="11">1.1.1.102</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">3-ketodihydrosphingosine reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">3-ketosphinganine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100842960</name>
        <name evidence="16" type="ORF">BRADI_3g52570v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>sphinganine + NADP(+) = 3-oxosphinganine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.102"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.102" evidence="11"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570174.1">
        <property type="nucleotide sequence ID" value="XM_003570126.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICY0"/>
    <dbReference type="STRING" id="15368.I1ICY0"/>
    <dbReference type="EnsemblPlants" id="KQK00903">
        <property type="protein sequence ID" value="KQK00903"/>
        <property type="gene ID" value="BRADI_3g52570v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842960"/>
    <dbReference type="Gramene" id="KQK00903">
        <property type="protein sequence ID" value="KQK00903"/>
        <property type="gene ID" value="BRADI_3g52570v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842960"/>
    <dbReference type="eggNOG" id="KOG1210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_3_2_1"/>
    <dbReference type="OMA" id="ICGVFEE"/>
    <dbReference type="OrthoDB" id="37659at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047560">
        <property type="term" value="F:3-dehydrosphinganine reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006666">
        <property type="term" value="P:3-keto-sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08939">
        <property type="entry name" value="KDSR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000165">
        <property type="entry name" value="3-ketodihydrosphingosine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045022">
        <property type="entry name" value="KDSR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550:SF3">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095205" description="3-dehydrosphinganine reductase" evidence="15">
        <location>
            <begin position="21"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK00903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="326" mass="34589" checksum="0901F3F13F4E8269" modified="2012-06-13" version="1">MASPLFLLLLLLVPPVGLLAALAFLVRPRAARIPIKGRHVFITGGSSGIGLAMATAAAREGAKVSILARNAARLEDARAAIRAATGHDVGVHQADVRDAGAVARALEEAGPVDVLVCNHGVFVAQELEKQDMEEVKWMVDINLMGTFHLIKAALPAMKARTRETRLPASIAIMSSQAGQVGVYGYTAYSASKFALTGLAESLQHEVISDNIHVSLIFPPDTETPGFAEELKKRPEITTIIAGSSGGMKADAVSKKALNGIKSGRFIVPCNFEGAMLAVATSGLTPQSSPLMAFVEVIGAGLMRFAALCFQWNWFSTIESWYANKHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1ID80</accession>
    <name>I1ID80_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100835414</name>
        <name evidence="8" type="ORF">BRADI_3g53380v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570234.1">
        <property type="nucleotide sequence ID" value="XM_003570186.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ID80"/>
    <dbReference type="EnsemblPlants" id="KQK01027">
        <property type="protein sequence ID" value="KQK01027"/>
        <property type="gene ID" value="BRADI_3g53380v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835414"/>
    <dbReference type="Gramene" id="KQK01027">
        <property type="protein sequence ID" value="KQK01027"/>
        <property type="gene ID" value="BRADI_3g53380v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835414"/>
    <dbReference type="eggNOG" id="KOG1237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009313_4_1_1"/>
    <dbReference type="OMA" id="CTPENMQ"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17419">
        <property type="entry name" value="MFS_NPF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="500"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK01027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="585" mass="64119" checksum="B571EA02A11F3668" modified="2012-06-13" version="1">MAPSIVDSKGISPITEDGSMDRRGNPAVKANTGKWKSSILLLVNYGLVTCAFFGVGVNLVVFLRRVLHQDNAEAANSISKWTGTVYIFSLIGAFMSDSYWGRYITCAIFQMIYVTGLVILSLTSWFLLVKPSGCGDGETHCDKPSTAGVALFYLSTYMIAFGNGGYQPSIATLGSDQFDETDPDEARSKVAFFSYFYLALNVGSLFSNTVLVYYEDSGQWVMGFWVSAAAAALALVLFLLGTPNYRYFKPSGNPLTRIAQVLVAASRKWRTHAPRGELLHEVDGDESKVSGIRKILHSDQLRYLDKAATVTEEEYFDPERMKDPWRLCTVTQVEEVKCILKMLPIWMCTIVYSVVFTQMASLFVEQGTTMDTNIVGSFHVPAASMSVFDILSVLAFIAIYRRVLVPVMARLSGNPQGLTELQRMGVGLVIGMGAMVVAGVVEVERLKRVAAPDQPSSLSVLWQVPQYALIGASEVFMYVGQLEFFNGQAPDGVKSFGSSLCMASISLGNYVSIMLVSVVTSLTAGERRPGWIPGNLNSGHLDRFYFLLAALSLVDLAVYVACAMWYKGIKLDNSNEEKGKVPAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDF8</accession>
    <name>I1IDF8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF295 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g54047v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDF8"/>
    <dbReference type="EnsemblPlants" id="PNT69339">
        <property type="protein sequence ID" value="PNT69339"/>
        <property type="gene ID" value="BRADI_3g54047v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69339">
        <property type="protein sequence ID" value="PNT69339"/>
        <property type="gene ID" value="BRADI_3g54047v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SBMB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019286_2_0_1"/>
    <dbReference type="InParanoid" id="I1IDF8"/>
    <dbReference type="OMA" id="HYESAGE"/>
    <dbReference type="OrthoDB" id="10617473at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF82">
        <property type="entry name" value="OS07G0500250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT69339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="29374" checksum="1F0DDB007BFCE430" modified="2012-06-13" version="1">MGDRLKAMPYFRIIDMAFLGDKLYAITMAEDLFVLHLGEDGDGKPTVTNVKHVIKHAPGHDDDRYYSWPSDVDLLGNEETDAELDGEAPSGDDNYGILSHEGVHDNDTVDHGSDTDNDGNDHLVFDDEWTLPVCEEGVCEGYDAIQITSRHLVEYLSCVVSCCWLSGCTMSQPSLQLTTLARLKCEAVDTCTWVPIVGVLGNGRAIFIGNRFSCAIPAYGEVEEDIMYFPDTDDVFDMRSGIIRPLTPIWTGCMIRVGHGCFHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJG0</accession>
    <name>I1IJG0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MCAfunc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC104584443</name>
        <name evidence="2" type="ORF">BRADI_4g10290v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758377.1">
        <property type="nucleotide sequence ID" value="XM_014902891.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJG0"/>
    <dbReference type="STRING" id="15368.I1IJG0"/>
    <dbReference type="EnsemblPlants" id="KQJ87309">
        <property type="protein sequence ID" value="KQJ87309"/>
        <property type="gene ID" value="BRADI_4g10290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584443"/>
    <dbReference type="Gramene" id="KQJ87309">
        <property type="protein sequence ID" value="KQJ87309"/>
        <property type="gene ID" value="BRADI_4g10290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584443"/>
    <dbReference type="eggNOG" id="ENOG502QQIG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100010_0_0_1"/>
    <dbReference type="OMA" id="RPYFVPF"/>
    <dbReference type="OrthoDB" id="606932at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21037">
        <property type="entry name" value="MLKL_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045766">
        <property type="entry name" value="MCAfunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059179">
        <property type="entry name" value="MLKL-like_MCAfunc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46604:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46604">
        <property type="entry name" value="PROTEIN MID1-COMPLEMENTING ACTIVITY 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19584">
        <property type="entry name" value="MCAfunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCAfunc" evidence="1">
        <location>
            <begin position="21"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ87309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="191" mass="22001" checksum="A6C1CCB30E54627A" modified="2012-06-13" version="1">MALVSQAAMVAQFAGVDAYGLIKMIAEAAQTVRRNRATCLQLARRAKMIGDLLHRLHAAQLMQQPETRNPVEQLEETLRRALLLIRSCRGRSYLYRCFMGARHADELREVQSEISFYLQLFPLISYVDTTLTWVRLLNKASPDTSCKEAPMVRPYFVPFLFLALDGWCLVVNYSGSIPWYSIHIVYIYFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMV8</accession>
    <name>I1IMV8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC104585044</name>
        <name evidence="9" type="ORF">BRADI_4g23550v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type IV zinc-finger family. Class A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239253.1">
        <property type="nucleotide sequence ID" value="XM_010240951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IMV8"/>
    <dbReference type="EnsemblPlants" id="KQJ89112">
        <property type="protein sequence ID" value="KQJ89112"/>
        <property type="gene ID" value="BRADI_4g23550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104585044"/>
    <dbReference type="Gramene" id="KQJ89112">
        <property type="protein sequence ID" value="KQJ89112"/>
        <property type="gene ID" value="BRADI_4g23550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104585044"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076470_0_0_1"/>
    <dbReference type="OMA" id="EEKRCGH"/>
    <dbReference type="OrthoDB" id="690813at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF79">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="259"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ89112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="357" mass="39024" checksum="88A3101782B6C0BD" modified="2012-06-13" version="1">MRKPGQYVYLHEDPLALDVVDGLDLSPPGGGLCSPDDPLDKVMTYVALLDDGFLEDLGIDCSTLLDEESSRSALEEGGGGALPVAAAAASPGGTKRSAREVFDGVPGAPSSIPVSTTSAGPFLGTLEAPDFGEDLSWTVLPKPHHRRTAARRRQVWPVSFPLALTTSPGPAAAAHDDSNANDDVKDKDFKLISRCNVGKCNGNKRRRLSEPKKRQRDKDNVEDKDFELVGYCNDVGVGNGKQRRSAEPKQQLDKDGRLRTCTHCCSWQTPQWRHGPNGNGTLCNACGIRYKMGKLCEEYRPSTSPEFSSLKHSNRHRNVEKIRDQKMKQLKVTSPVPVVPVTPDSSEVLLRMCKYET</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1INA6</accession>
    <name>I1INA6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vacuolar iron transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100830351</name>
        <name evidence="11" type="ORF">BRADI_4g24820v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Vacuolar Fe(2+) uptake transporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Fe(2+)(in) = Fe(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28486"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:28487"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576279.1">
        <property type="nucleotide sequence ID" value="XM_003576231.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INA6"/>
    <dbReference type="FunCoup" id="I1INA6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1INA6"/>
    <dbReference type="EnsemblPlants" id="KQJ89313">
        <property type="protein sequence ID" value="KQJ89313"/>
        <property type="gene ID" value="BRADI_4g24820v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830351"/>
    <dbReference type="Gramene" id="KQJ89313">
        <property type="protein sequence ID" value="KQJ89313"/>
        <property type="gene ID" value="BRADI_4g24820v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830351"/>
    <dbReference type="eggNOG" id="KOG4473">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038957_5_1_1"/>
    <dbReference type="OMA" id="WFVAGAY"/>
    <dbReference type="OrthoDB" id="696129at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140315">
        <property type="term" value="F:iron ion sequestering activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ89313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="220" mass="22129" checksum="A4C500E6AAD6A6E7" modified="2012-06-13" version="1">MATSNNTKLAVQELSHRTIVPETGKPCPACVAGYDAMSTSPQHGQWLRAAVLGASDGLVSTAALMLGIGAARPADPRAALLSGVAGLVAGACSMAIGEYVSVHAQLDVELAGLKQVEEARGSSMDRAGLPSPSQAAAASAMSFAVGAAIPLLVAWFVASYKVRVVVVVVTATLTLAVFGTLGAVKGQAPGGRAGLRAAMGGLVAMGITYGLMKLFRTHSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITF1</accession>
    <name>I1ITF1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tyrosine N-monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_4g39657v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITF1"/>
    <dbReference type="EnsemblPlants" id="KQJ91776">
        <property type="protein sequence ID" value="KQJ91776"/>
        <property type="gene ID" value="BRADI_4g39657v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ91776">
        <property type="protein sequence ID" value="KQJ91776"/>
        <property type="gene ID" value="BRADI_4g39657v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_4_0_1"/>
    <dbReference type="InParanoid" id="I1ITF1"/>
    <dbReference type="OMA" id="INERWRQ"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000037">
        <property type="entry name" value="Cytochrome P450 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944:SF4">
        <property type="entry name" value="OS09G0441700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 10" id="KW-0349">Heme</keyword>
    <keyword evidence="7 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ91776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="59738" checksum="1F33D9FEA4D36D8B" modified="2012-06-13" version="1">MLRSFSSVAVAQLLLVATFLYLLQRFLPRRSCRIFSAPPVGPGPRLLPPGPSPWPVVGSLPEMTLNKPAFRWIHRVMHELGTDIACFRLGGVHVIPVTCPRIAREVLQKQDANFASRPLSFASGAVSGGYVDVVLAPFGEQWKNMRRVVLSEIVCPSRHNWLHERRAQEADNLTRYAYSLALQGTTVDVRHVAQHYCGNVIRRLVFGRRYFGGGGIEDGGPGPLEVEHVDASLATLGMVYAFCVSDYLPCLLGLDLDGHEKKVREALVKIDRLHDAVILERWRQWKSGERRPEEVEDFLDILISFKGALLSIDEVRALCRDIVFAAVDNPSNAVEWTMAEMVNRPELLEKAVEELDQVVGRDRLVQESDIPKLQYAKACIREAFRLHPVAPFNVPHVALADATVAGYHIPKGSHVMLSRVGLGRNPAVWPDPLRFDPERHLVNDDVALTENELRFISFSTGRRGCMAVWLGTAMSVMLFGRLLQGFTWSKPEGVEVIHLKEGKHDLFMEKPLLLHAEPRLPVHLYRAAAQPVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITM1</accession>
    <name>I1ITM1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100835474</name>
        <name evidence="12" type="ORF">BRADI_4g40287v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578788.1">
        <property type="nucleotide sequence ID" value="XM_003578740.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITM1"/>
    <dbReference type="SMR" id="I1ITM1"/>
    <dbReference type="STRING" id="15368.I1ITM1"/>
    <dbReference type="EnsemblPlants" id="KQJ91878">
        <property type="protein sequence ID" value="KQJ91878"/>
        <property type="gene ID" value="BRADI_4g40287v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835474"/>
    <dbReference type="Gramene" id="KQJ91878">
        <property type="protein sequence ID" value="KQJ91878"/>
        <property type="gene ID" value="BRADI_4g40287v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835474"/>
    <dbReference type="eggNOG" id="KOG0935">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061221_3_1_1"/>
    <dbReference type="OMA" id="QSNFVEY"/>
    <dbReference type="OrthoDB" id="371463at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14833">
        <property type="entry name" value="AP2_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000004">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027156">
        <property type="entry name" value="APS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="11">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ91878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="142" mass="17066" checksum="EA72FAD9E09EA533" modified="2012-06-13" version="1">MIRFILLQNRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKFTNFVEFRTHKVIYRRYAGLFFSICVDITDNELVYLECIHLFVEILDHFFSNVCELDLVFNFHKVYLILDEFILAGELQETSKKAIIERMGELEKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVK7</accession>
    <name>I1IVK7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4220 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100843617</name>
        <name evidence="3" type="ORF">BRADI_5g01050v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ81488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="BRADI_5g01050v3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="12"/>
            <end position="34"/>
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        <location>
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        <location>
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            <end position="277"/>
        </location>
    </feature>
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        <location>
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            <end position="322"/>
        </location>
    </feature>
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        <location>
            <begin position="48"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ81488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="639" mass="72794" checksum="7661F4BF0EDEBFF5" modified="2012-06-13" version="1">MAGWPIDLWNEWAIQILVLLSFMLQVVLFVFAGIRRREASPVLRLLLWLAYQLADSTAIYALGLLSLNSFPREHQLVPFWAPFLLLHLGGPDNITAYSLEDSKLWKRHLLTVFVQVLGAAYVLYKHIAGRGNLVLAAIFMFVVGVVKYAERIWALRCSTMDSIRSSLNNSRGRNDHYTIPNGVKGKFHEQVLLELAHSLFHFCKLKLVDSSVNMDHPNLVRSRLDWSDIWKLIEMELSLLYDILYTKAAVVHTWYGYCIRFMTPFATAGALLLFHFSNGLGGLSGVDVAITYALLGGALFMEVTALLGAIGSTWTLAFLCALPMRCSWLRHALVCSGRWHRLRRLLLTLSRLDIRTRRRWSGTIGQYNLLHLCACGSSDPLRRLAEILGLKERWNKLHYSRTLVVSEVVKKLVFNRHGTLVSRSSHVNALGLLRAKWGKWALAKNELYDEVFKDCDHLLGVELQEGILIWHIATDVFLANRENKEDTAAAHDVVAAIKALSNYMMFLLVRRPDLLPGLVLNRIYQLTCENLVEIWHGSLQREKLAVILYREKKTLDFNSSELRVRYGVEVAEKLIQQEKAGPTDILGVLLDVWMDFLSYAANRCSRESYVNRLNNGGELIAILWLMAAHFEPQNRASSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1J089</accession>
    <name>I1J089_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_5g17370v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J089"/>
    <dbReference type="FunCoup" id="I1J089">
        <property type="interactions" value="1989"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J089"/>
    <dbReference type="EnsemblPlants" id="KQJ83883">
        <property type="protein sequence ID" value="KQJ83883"/>
        <property type="gene ID" value="BRADI_5g17370v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83883">
        <property type="protein sequence ID" value="KQJ83883"/>
        <property type="gene ID" value="BRADI_5g17370v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="I1J089"/>
    <dbReference type="OMA" id="ITMENGP"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J089">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GATA26/27"/>
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        <property type="entry name" value="Znf_GATA"/>
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        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46855:SF1">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46855">
        <property type="entry name" value="OSJNBB0038F03.10 PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
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    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="3">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="40"/>
            <end position="113"/>
        </location>
    </feature>
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            <end position="361"/>
        </location>
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        <location>
            <begin position="418"/>
            <end position="443"/>
        </location>
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        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
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        <location>
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            <end position="104"/>
        </location>
    </feature>
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        <location>
            <begin position="340"/>
            <end position="352"/>
        </location>
    </feature>
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        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ83883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="443" mass="48601" checksum="9B8D47051DE79ACC" modified="2012-06-13" version="1">MVKEGPCRHCGVTSTPLWRNGPADKPVLCNACGSRWRTKGSLENYTPMHSRDDIDVEEPRVSKLKPPMSRLKEQRQLKKRPSHIIKKNEPFSDQNFRKMGDADPSRSSSGSAVSYSESCAPYGSADASEMTGSAQSHAWESLVPSRKRSCVTRSKPSQVEKLVKDLNSIMHEEQFYCLSGSSEEDLLYHSETAVGSFEIGYGSVLLRHANSKSVDGDSEANSVPADNKSYVTSESLSYSGTASFVVHGESKGASNSNALSEKPKWFPVQIHDNARRDKLHYSKPHTLENVDSALVSVALEVKDSKEIGEKENISAVKCLVKPAMKHLKRPHESQLQSCQEVKETTRSPKRGSESGAMAPQFKGSFLPKSGGALNLFMLPPDKLSMLAPQYVDDSDQDLLLEVPPNGRHPEAELLCPLSQLSSVSRSSTSEGRVADGDGRLRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0I4</accession>
    <name>I1J0I4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
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        <name evidence="12" type="ORF">BRADI_5g18170v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="KQJ84016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240237.1">
        <property type="nucleotide sequence ID" value="XM_010241935.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0I4"/>
    <dbReference type="SMR" id="I1J0I4"/>
    <dbReference type="STRING" id="15368.I1J0I4"/>
    <dbReference type="EnsemblPlants" id="KQJ84016">
        <property type="protein sequence ID" value="KQJ84016"/>
        <property type="gene ID" value="BRADI_5g18170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844431"/>
    <dbReference type="Gramene" id="KQJ84016">
        <property type="protein sequence ID" value="KQJ84016"/>
        <property type="gene ID" value="BRADI_5g18170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844431"/>
    <dbReference type="eggNOG" id="KOG0223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_3_0_1"/>
    <dbReference type="OMA" id="VANHWNH"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000001">
        <property type="entry name" value="Probable aquaporin PIP1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45687">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ84016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="30563" checksum="3527D0DEC207EE21" modified="2012-06-13" version="1">MEGKEEDVRLGANKFSERQPIGTAAQGSEDKDYKEPPPAPLFEPGELKSWSFYRAGIAEFMATFLFLYVTILTVMGVNNSPSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYIVMQCLGAICGAGVVKGFQSGLYMSSGGGANAVAAGYTKGDGLGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNKSHNWADHWIFWVGPFIGAALAAVYHQVVIRAIPFKTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>I1J2L1</accession>
    <name>I1J2L1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g23992v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT62009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J2L1"/>
    <dbReference type="EnsemblPlants" id="PNT62009">
        <property type="protein sequence ID" value="PNT62009"/>
        <property type="gene ID" value="BRADI_5g23992v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62009">
        <property type="protein sequence ID" value="PNT62009"/>
        <property type="gene ID" value="BRADI_5g23992v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2362624_0_0_1"/>
    <dbReference type="InParanoid" id="I1J2L1"/>
    <dbReference type="OrthoDB" id="9008980at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003645492" evidence="1">
        <location>
            <begin position="29"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT62009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="96" mass="9482" checksum="ED3AA83AB4795F2B" modified="2012-06-13" version="1">MEFRGVARATTCTLGVLLTAMLIGGANAGGDANHDVELQSLLTCIVGCATELTGCSTMCVNLPLGEASGCAIGCIDGNTKCLVNCAMPAPPPPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1J2U3</accession>
    <name>I1J2U3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_5g24622v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT62047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J2U3"/>
    <dbReference type="FunCoup" id="I1J2U3">
        <property type="interactions" value="1622"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J2U3"/>
    <dbReference type="EnsemblPlants" id="PNT62047">
        <property type="protein sequence ID" value="PNT62047"/>
        <property type="gene ID" value="BRADI_5g24622v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62047">
        <property type="protein sequence ID" value="PNT62047"/>
        <property type="gene ID" value="BRADI_5g24622v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S8AI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197225_0_0_1"/>
    <dbReference type="InParanoid" id="I1J2U3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37225:SF1">
        <property type="entry name" value="OS04G0657900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37225">
        <property type="entry name" value="OSJNBA0011F23.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT62047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="69" mass="7811" checksum="7EE388283B19110B" modified="2012-06-13" version="1">MEGAAAQIVFPILGIVAAAAATFYAVSFMEIREKSLEELDEKYSEYEESGGRQRRARRRSGRQAKKRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E8M7</accession>
    <name>A0A0Q3E8M7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g10539v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82700.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E8M7"/>
    <dbReference type="EnsemblPlants" id="KQJ82700">
        <property type="protein sequence ID" value="KQJ82700"/>
        <property type="gene ID" value="BRADI_5g10539v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82700">
        <property type="protein sequence ID" value="KQJ82700"/>
        <property type="gene ID" value="BRADI_5g10539v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E8M7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ82700.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="175" mass="19299" checksum="18CFBD3AD5FD6803" modified="2018-03-28" version="2">MGYMARYFWYVRTHVGPFLPSTRSLYRIYVDCHRFSSPSLHGRSVQRKEISTAASLPLLIPIWIASLLSPHIPIWIAAATSPPAAGAMAHPLVPSYYRIPLFRCNLAGPEDQPRLLNPPPPSTSQDQLRPPCPAASLSRPRPPYPPPPHPRTLPKLSPAAPPAAATFSCRTARLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EB41</accession>
    <name>A0A0Q3EB41_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100846783</name>
        <name evidence="3" type="ORF">BRADI_5g15647v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751280.1">
        <property type="nucleotide sequence ID" value="XM_014895794.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311308.1">
        <property type="nucleotide sequence ID" value="XM_024455540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EB41"/>
    <dbReference type="STRING" id="15368.A0A0Q3EB41"/>
    <dbReference type="EnsemblPlants" id="KQJ83588">
        <property type="protein sequence ID" value="KQJ83588"/>
        <property type="gene ID" value="BRADI_5g15647v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846783"/>
    <dbReference type="Gramene" id="KQJ83588">
        <property type="protein sequence ID" value="KQJ83588"/>
        <property type="gene ID" value="BRADI_5g15647v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846783"/>
    <dbReference type="OrthoDB" id="682414at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF307">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MULE transposase" evidence="2">
        <location>
            <begin position="77"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ83588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="361" mass="42353" checksum="879138439F4D207E" modified="2016-01-20" version="1">MDSIPFTKRSLKSLCGKISWEQSDNDAVKTMEVFGKLLDEDPDFKYMVQLDGESRMRTLLWTSARCSEKYACFGDAITFDTTYRTNLYNMPFGLFVGVNNHFQSIILRGVLMRDEKEDSFRWVFREFMRMIGGKDNHPKTILTDQARAMELAIAEELPNTTHRWCKWHVLKKAKECLGALYGKRTSFRAEFHRLVSEQYTEEEFEQSWAEMLNKHGLQNQPYLTQIYEVRHKWAKPYFRKTFCAKMTSTQRSESANHMLKTYIPPGCPMHLFVKQYAKLQFNRDQEESYQEKRTALSGVVLKSNWPIDRHAGKIYTRAMFEQFGICLYESGHYLVDEVEPDAVYIAALNGFFKGKMVQSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F7X9</accession>
    <name>A0A0Q3F7X9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g19611v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F7X9"/>
    <dbReference type="EnsemblPlants" id="KQJ95887">
        <property type="protein sequence ID" value="KQJ95887"/>
        <property type="gene ID" value="BRADI_3g19611v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95887">
        <property type="protein sequence ID" value="KQJ95887"/>
        <property type="gene ID" value="BRADI_3g19611v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3F7X9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ95887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="73" mass="8007" checksum="4A354317A8418602" modified="2016-01-20" version="1">MFSPLLPNASSRRCLPSGLSLQISLQLSDVGMCLGLSNAGCQEFQHEGTCKGKENRITLLPKLLVCCNFYKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GMM7</accession>
    <name>A0A0Q3GMM7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="18 19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BRADI_1g03200v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function as sodium-coupled metabolite transporter across the chloroplast envelope.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12360.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GMM7"/>
    <dbReference type="EnsemblPlants" id="KQK12360">
        <property type="protein sequence ID" value="KQK12360"/>
        <property type="gene ID" value="BRADI_1g03200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12360">
        <property type="protein sequence ID" value="KQK12360"/>
        <property type="gene ID" value="BRADI_1g03200v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GMM7"/>
    <dbReference type="OrthoDB" id="2687058at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
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    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
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    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
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            <end position="63"/>
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    <sequence length="805" mass="84966" checksum="6301B368F8627063" modified="2018-03-28" version="2">MAPAGGGGVRREEADEEAGAYVPIRSTSEGVWQGDDPLRHSLPLLLLQICLVVVVTRSLAFALRPLRQPRVIAEIIGGMLLGPSALGRSKMFLDNVFPRESLTVLDTLANIGLLFFLFLVGLELDPASLKRTGRSALAIAVAGISLPFSLGVGSSLVLRDAVAPDAPRGPFIVFMGVALSITAFPVLARILAELKLLTTELGRMAMSAAAVNDVTAWILLALAIALSGSGSPLASVYVLLSGAAFVAAAVLLVRPLLVHMARRSPDGEPVKESFVCAAMTIVLAAGLTTDTIGIHALFGAFVIGVLVPKEGAFAGALTEKIEDLVSSLLLPLYFVSSGLKTNVGTISGAKSWGLLLLVITTACVGKIGGTVLTSLLMRVPVREAVALGLMMNTKGLVELIVLNIGRDRKVLNEEAFAILVLMALVTTFLTTPAVTAVYKPARRQACYTHRTVERDDADADSELRVLACFHASRGIPTLINLVEASRGTRRSKLTMYAMHLVELSERSSAISMVQRARRNGLPFSSRGKQGGGGGGEVVQVAFEAFQRLSAVKVKPMTAISDLATIHEDIVASAVHKRAALIVLPFHKMLSHDGTALEPLDRAYHHVNVRVLRKAPCSVAVLVDRALGGMAQVSAPEVSYSVLLLFFGGPDDREALAYASRMAEHPGIALTVARFTGSTAPGDHDKPAAAAAAEVVPMDEEAIRKYVVVSGSVRYEEVAAEGRQEVAAAIKAMGRGKNLVVTGRSARAAPVLVEKSDCPELGPVGSYLATAEFSATASVLVVQRYDRRSDPTSTPAAVICTWPVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GU79</accession>
    <name>A0A0Q3GU79_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100836312</name>
        <name evidence="22" type="ORF">BRADI_5g21870v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT61854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010242123.3"/>
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        <property type="protein sequence ID" value="KQJ84618"/>
        <property type="gene ID" value="BRADI_5g21870v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT61854"/>
        <property type="gene ID" value="BRADI_5g21870v3"/>
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    <dbReference type="GeneID" id="100836312"/>
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        <property type="gene ID" value="BRADI_5g21870v3"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Galactose-binding lectin"/>
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    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
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        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
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        <property type="entry name" value="Malectin_dom"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS04G0616500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5006203245" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="21"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="627"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="685"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="983"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="983"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="713"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KQJ84618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1015" mass="110244" checksum="D04A1111AAE612CD" modified="2016-01-20" version="1">MRRLHGAALLLLLLAAVAQAQQASTTHPGDAAALNAVFAKLGQKAGPSWNISGDLCTGAATDTTDIDNDNSFNPAIKCVCSGGNTSVCRITRIKIYALDAVGPIPEELRNLTSLTNLNFGQNYLTGPLPSFIGELTAMQYMTFGINALSGPVPKELGNLTNLVSLSISSNNFSGPLPSELGNLAKLEQLYIDSAGLSGSLPSSLSKLTKMKTLWASDNDFTGQIPDYIGSWNLTELRFQGNSFEGPLPATLSNLVQLTGLRIGDILNGSSSSLAFLNNMTSLSTLVLRNCRISDKLVSVDFSKFRNLNLLDLSFNNITGQVPQTLLNLNSLNFLFLGNNSLSGSLPSSIGSSLKNLDFSYNQLSGSVPSWARSSQLNLVANNFLADSSGNSVLPTGWECLQRNTPCFLGSPQSSSFAVDSGGNGLISGSDNSMYQPDDIGTKLGAASYYVTGAPTWGVSNVGRFMDASNGSYIIYSSQQFQNTLDTELFRNARMSPSSLRYFGIGLENGNYTVTLQFAEFDFPDSQSWKSTGRRVFDIYVQGERKEQNFDIRKAAGGKSYIAVRRQYTIKVTKNFLDIHLFWAGKGTCCIPTQGYYGPAISALSATPNFTPTVRNAAVKKSGSNTGVIVGVLVAVAVLGLLAVAGIFIWRQKRRKLLLEQQELYSIVGRPNVLSYAELRSATENFSSSNLIGQGGYGSVYKGKLVDGRLVAVKQLSEASHQGKREFAAEIETISRVQHRNLVKLYGCCLEGNKPLLVYEYLENGSLDKALFGKGSSNLDWPTRFEICLGIARGLAYLHEESSIRVVHRDIKASNVLLDANLNPKISDFGLAKLYDDQKTHVSTKVAGTFGYLAPEYAMRGHMTEKVDVFAFGVVLLETLAGRPNYYSTLDEDKVYIFEWVWELYEANHPLDVVDPRLEEFDSEEVLRAIKVALVCTQGSPHQRPSMSRVVAMLTGDVEAPDVVSKPSYITEWQIKGGDTTSYMSTDVSAQSSSAPRPNSPRTSSPFLSSIIDEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H3E1</accession>
    <name>A0A0Q3H3E1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100844853</name>
        <name evidence="3" type="ORF">BRADI_1g34150v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563509.1">
        <property type="nucleotide sequence ID" value="XM_003563461.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H3E1"/>
    <dbReference type="STRING" id="15368.A0A0Q3H3E1"/>
    <dbReference type="EnsemblPlants" id="KQK17391">
        <property type="protein sequence ID" value="KQK17391"/>
        <property type="gene ID" value="BRADI_1g34150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844853"/>
    <dbReference type="Gramene" id="KQK17391">
        <property type="protein sequence ID" value="KQK17391"/>
        <property type="gene ID" value="BRADI_1g34150v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844853"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H3E1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024593">
        <property type="entry name" value="DUF3444"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47374:SF9">
        <property type="entry name" value="DUF3444 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47374">
        <property type="entry name" value="ENDOSOME ANTIGEN-LIKE PROTEIN, PUTATIVE (DUF3444)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11926">
        <property type="entry name" value="DUF3444"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="67"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK17391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1043" mass="117264" checksum="3AAF3F0FD68393F9" modified="2016-01-20" version="1">MMECNREEAFRAREIALKQMENEDFIGAQKIALQAQMLFPELEDISKLLNICNVHCAAEARVNGETDWYAILQVEPTADPDHIKKQYHRLAKSVHPDKNCFSGAEAAFKLVSEAYTVLFDQTKRSHYDIRRLNALRKVRKQATQQQKKSDTSKSDVPGYVASFWTICPHCETQYQYPIYVLNTVMCCLSCRKNYFAYNLKEQYVPTSSSVPSSFQVPAKMFPSQQGHPVKLSSVRGTTYVKPRMNVAHCDEYMKGYSRSSMDEKANQSQSGTRVGNQFSAMNQDKSSVPTVNAHVGERSIPIPADPDIIGIQKSGTEEASAAPNAKKVPGPVKLSSTGVNTYTKVKINVARCDIKGNGSTGGDKEANQSDITSRKVEIPTMNQAKSSAQTTNINTDGRWMPNPADPNVDRNNLWKEDISTVSDGAGSSSLRRSARRKQDAAGSSNLDSNSKKKQRINDLQSSDLNCKNMLDDNVTNADRQSVPPHVSSKIDIQEKAKTRDMGDQDNINAEATDTVGQNEQSYSVKLSLPDTDFFDFEKLRDVNMFAVGQIWAIYDNLDGMPRYYARIKRFDASNFNVHLTWLEYIEANEAEKKWTDEEMPVACGSFRLGTADISQDRPMFSHIVSGAKCKKRNYEIHPSKGEVWALYKGWSLQWGSDADNHRSYEYEVVEVLSNVSVSAGVSVVPLVRIKGFVSLFATAKDKSEIVIASSELLRFSHSIPFYRTNGNEKTGVPGGFLELDTACLPVDLDAAFPSVTLESFMSPCNKDDITFTDFCGDGAIGKDEDIFSEQNTSLPKTAHGCNGLGASSTQNCPSPRIYTYPDSEFHNFEEFRSCENFQCGQIWALYSDLDYFPKFYGWISKVELEPFEVHLTWLEACPQLKQEKQWLKQDIPLSSGNFKIRNWETKYETNDTFSHLVYAGPIESNQQIRILPQVGEIWAIYMNWTPDWTPSSIDACEFAMGEIIERTEASTKVSLLAQVDGYRAVFKLDKRKVALEIPSRDNQKFSHRVPSFRLTEESGGKLRGFYELDPASVPDVFLYRDAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HRF8</accession>
    <name>A0A0Q3HRF8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g34053v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HRF8"/>
    <dbReference type="EnsemblPlants" id="KQJ90819">
        <property type="protein sequence ID" value="KQJ90819"/>
        <property type="gene ID" value="BRADI_4g34053v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ90819">
        <property type="protein sequence ID" value="KQJ90819"/>
        <property type="gene ID" value="BRADI_4g34053v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HRF8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ90819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="87" mass="9290" checksum="16D19DF49F614169" modified="2016-01-20" version="1">MEDRGRDPPSVDHRCTADAEGSLPPFSPPATPPLPLPSPFSPVLVPSPPVAVLGRMEPPRIWSSGRRSVAGGDTSALGRKQEGRRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HX57</accession>
    <name>A0A0Q3HX57_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g34707v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HX57"/>
    <dbReference type="EnsemblPlants" id="KQJ98058">
        <property type="protein sequence ID" value="KQJ98058"/>
        <property type="gene ID" value="BRADI_3g34707v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ98058">
        <property type="protein sequence ID" value="KQJ98058"/>
        <property type="gene ID" value="BRADI_3g34707v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HX57"/>
    <dbReference type="OrthoDB" id="1069523at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF83">
        <property type="entry name" value="OS08G0371608 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="517"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ98058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="529" mass="59069" checksum="AF60F0A9CD94584A" modified="2016-01-20" version="1" fragment="single">ITSTFKELQQHLTTRLGEASDAIGSVSKTFLDAFVDSTFTFSHQPLRSTESNFAPVDENGESIAILDIEGTIPADFPEGVYVRNGSNPLFGALHVVRSIFGQSEDIWVEGEGMLHALHFTRGCNGTVDEWSVSYTNRYVQSDTFTVERARQRPCFFSVTKGNPLATLAASILNMLRFGKVIRNMSNTSVFVHAGRVFAAAENDSPHEMDLDSLDTMGSWSIGGDWVMPFTAHPKVVPGSGELVIYGINIMKPYLTVGVVSERCAFCHEIGITKMYNIIMDMPLTLSACRILRAATFIDYENESYARIGVMPRYGDAESVIWFAVEPFCTLHLVNCFEEGDEIIVRGFRVPASIIMGPTLQCNEESGDQELNEEYFSRLYEWTLNLKSRAVTGKWLTGTDVALEFPVINDKYVGLHHSNLYTLVVRPKFGGFAKLYLDQKRKVQINVEYHHLGRNQFCSGATFVPNVNAVHEDDGWIISFVHDEGTNISQAHIISAQRFESGPVAKITLPHRVPYGFHGAFVSRNIKEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I2F7</accession>
    <name>A0A0Q3I2F7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100841860</name>
        <name evidence="8" type="ORF">BRADI_3g46965v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317230.1">
        <property type="nucleotide sequence ID" value="XM_024461462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I2F7"/>
    <dbReference type="STRING" id="15368.A0A0Q3I2F7"/>
    <dbReference type="EnsemblPlants" id="KQK00043">
        <property type="protein sequence ID" value="KQK00043"/>
        <property type="gene ID" value="BRADI_3g46965v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841860"/>
    <dbReference type="Gramene" id="KQK00043">
        <property type="protein sequence ID" value="KQK00043"/>
        <property type="gene ID" value="BRADI_3g46965v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="690947at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044545981" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="6">
        <location>
            <begin position="22"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="122"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK00043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="180" mass="18378" checksum="BF0CDDA842943989" modified="2016-01-20" version="1">MRCSAALIFFLLAVSRLCVLAVVIRADAGASASIPAPAPAPAPDPLLPCLEEVLPCTAYLKSAKRPAQTCCTALSRAAGAGMPCLCRLLADPGMLFTFNVTREQTLRLPSRCGLPVGCRSSATGTSEPVVEAPPPPPAVPPPRRRVPDPSSNGGGRCGTGARRAIVTVLLGGLVSIALLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I6H9</accession>
    <name>A0A0Q3I6H9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">adenylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100829558</name>
        <name evidence="14" type="ORF">BRADI_5g02200v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="3"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0A0Q3I6H9"/>
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    <dbReference type="STRING" id="15368.A0A0Q3I6H9"/>
    <dbReference type="EnsemblPlants" id="KQJ81672">
        <property type="protein sequence ID" value="KQJ81672"/>
        <property type="gene ID" value="BRADI_5g02200v3"/>
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    <dbReference type="Gramene" id="KQJ81672">
        <property type="protein sequence ID" value="KQJ81672"/>
        <property type="gene ID" value="BRADI_5g02200v3"/>
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    <dbReference type="OrthoDB" id="442176at2759"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="expression patterns" value="baseline and differential"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:UMP kinase activity"/>
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        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
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        <property type="term" value="P:CDP biosynthetic process"/>
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        <property type="term" value="P:UDP biosynthetic process"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
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        <property type="entry name" value="Adenylat_kinase_CS"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR006266">
        <property type="entry name" value="UMP_CMP_kinase"/>
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        <property type="entry name" value="UMP_CMP_kin_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ81672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="249" mass="27626" checksum="B6101492D1FEDC83" modified="2016-01-20" version="1">MWRRRLGALLLRSPSSSTPSSSSSYQQHHHHLIPTPNEKPLALSLLRLFASQAGSDGGRPFIAFILGGPGSGKGTQCTRIASDFGFAHVSAGDLLRNEISSGTDKGELILEIIKEGRIVPSEITVELIRKAIESTTAKRVLIDGFPRCEENRIAFEKITGTEPDLVIFFDCPEDEMVKRLLGRNQGRVDDNIETIKKRLKVFESLNIPVVEYYSSRGKAHKINATGTEDEIFEAVRKLFSSLRATYLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IKU7</accession>
    <name>A0A0Q3IKU7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_2g26342v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06435.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IKU7"/>
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        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3IKU7"/>
    <dbReference type="EnsemblPlants" id="KQK06435">
        <property type="protein sequence ID" value="KQK06435"/>
        <property type="gene ID" value="BRADI_2g26342v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06435">
        <property type="protein sequence ID" value="KQK06435"/>
        <property type="gene ID" value="BRADI_2g26342v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IKU7"/>
    <dbReference type="OrthoDB" id="650089at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IKU7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF13">
        <property type="entry name" value="OS09G0290500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="5">
        <location>
            <begin position="504"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK06435.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="606" mass="71292" checksum="1DB1EA3D2183DFF6" modified="2018-03-28" version="2" fragment="single">MEEMEEYHMMVSQMFGSVEEGYEHYNSYAKSKGFNVRLDDKEYISGTTELKRRRFCCSKEGYRLEKYFVAKDQIREPRALTRCGCKAMLEIQREPGTGLWFVKNFVDVHNHPLDPKQSPFLRSHRRLNNAQKADAVEYGLGGLRTCEIMEVMEKHHGGYDQVGFVSRDLYNFFAKYKKKRILGRDAEFVLNHMRAQVERDAEFFFKYSTDDEGHLRNIFWADSQSQIDYEAFGDLVVFDSTYRVNRYNLPFVPFIGVDHHRSTIVFGVGIVSDETVSSYEWLLQSFLEVMSHKNPRSVITDGDAAMRKAIKKVMPRTDHRLCSWHIEQNMIRHLRNPMLRDFRKLIYRKMGVYEFERSWAQFKEKYEITEQSAWMSRMYKLRKRWSAAYTNGRYFLGMLHNHLDRKMSLVDLVEHSEHCMSRIRRNEDELDAKAANSVPFTRIDANPLEKNAARIYTPKMFKKFRYCIRRSSAWEIEEHTERDGLVTYRDALKEGAEGGSRHVFFVECSFHGSSMNGIFCGCRKLECEGVPCSHIFSVLSFLGVETIPLCCVWIRWTMQAKAAFVSETNGNTHVWSEEMGRYRQMRNKASVALFKVVEWAEEAKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IWK3</accession>
    <name>A0A0Q3IWK3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_2g16271v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The LHC complex consists of chlorophyll a-b binding proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Plastid</location>
            <location evidence="2 11">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IWK3"/>
    <dbReference type="STRING" id="15368.A0A0Q3IWK3"/>
    <dbReference type="EnsemblPlants" id="KQK04836">
        <property type="protein sequence ID" value="KQK04836"/>
        <property type="gene ID" value="BRADI_2g16271v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04836">
        <property type="protein sequence ID" value="KQK04836"/>
        <property type="gene ID" value="BRADI_2g16271v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IWK3"/>
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        </ligandPart>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
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    </evidence>
    <sequence length="225" mass="23820" checksum="B879D1421C018480" modified="2016-01-20" version="1">MAATTMSLSSSAFAGNTVKNLPSAALFGEARVTMRKTAAKAKPVSSGSPWYGSDRVLYLGPLSDEPPSYLTGEFPGDYGELEVIHSRWAMLGALGCVFPELLAHNGVKFGEAVWFKIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGTVEGYRVAGGPLGEIVDPLYPGSSFDPLGLADDLEAFAELKAIVTGKGLLENLADHLADPVNNNPWAFATNFIPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J140</accession>
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            <taxon>Poaceae</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">Bd21</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">cv. Bd21</strain>
        </source>
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        <property type="component" value="Chromosome 4"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ92064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="207" mass="23620" checksum="C420225A408D48A4" modified="2016-01-20" version="1">MASHTLSWMIVVCLLAFLLASSGPVPARAQTDGFSEKLWNLPERARYRFDGTVHRMWIFPSDKPYNQGGSTKPRTEMQITGHDYNSGVWQFEGSLFVPFGSSGMSVMQIFGGNPTATTLMLHAYDGNLWYYNQKFVEANISNRWIRVNVIHDVDTSKLTVFINGQLKLTVNGKGGDNHFFKFGVYEQRNPTARMEARWMNVRILKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J1Z5</accession>
    <name>A0A0Q3J1Z5_BRADI</name>
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        <recommendedName>
            <fullName evidence="6">Phospholipase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100831328</name>
        <name evidence="10" type="ORF">BRADI_2g27947v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="6"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231500.1">
        <property type="nucleotide sequence ID" value="XM_010233198.3"/>
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    <dbReference type="STRING" id="15368.A0A0Q3J1Z5"/>
    <dbReference type="EnsemblPlants" id="KQK06708">
        <property type="protein sequence ID" value="KQK06708"/>
        <property type="gene ID" value="BRADI_2g27947v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831328"/>
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        <property type="gene ID" value="BRADI_2g27947v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831328"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Endonuclease Chain A"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF197">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
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    </dbReference>
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    <dbReference type="Pfam" id="PF00614">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="236"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="9">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="9">
        <location>
            <begin position="924"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        <source>
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            <dbReference type="PROSITE" id="PS50035"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="KQK06708.1"/>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK06708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K4P8</accession>
    <name>A0A0Q3K4P8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g48418v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK19468.1"/>
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    </dbReference>
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        <property type="protein sequence ID" value="PNT76465.1"/>
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    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQK19468">
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        <property type="gene ID" value="BRADI_1g48418v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT76465">
        <property type="protein sequence ID" value="PNT76465"/>
        <property type="gene ID" value="BRADI_1g48418v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT76466"/>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="BRADI_1g48418v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K4P8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK19468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="131" mass="13865" checksum="ABEC20975FA9D232" modified="2016-01-20" version="1">MRPLPSSARCRPARRCPAPPPARRWYCRLAPDTCESCCGRPQICRPQPNPDFSIVPKPNSQARITRPPSLGLANCPSHPLLDLGEAARAGGAGGGMCRAQVAQGQAACSGPVRALAGSGSAWWARAARKWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K7Z6</accession>
    <name>A0A0Q3K7Z6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_2g31860v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K7Z6"/>
    <dbReference type="STRING" id="15368.A0A0Q3K7Z6"/>
    <dbReference type="EnsemblPlants" id="KQK06977">
        <property type="protein sequence ID" value="KQK06977"/>
        <property type="gene ID" value="BRADI_2g31860v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT71595">
        <property type="protein sequence ID" value="PNT71595"/>
        <property type="gene ID" value="BRADI_2g31860v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06977">
        <property type="protein sequence ID" value="KQK06977"/>
        <property type="gene ID" value="BRADI_2g31860v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71595">
        <property type="protein sequence ID" value="PNT71595"/>
        <property type="gene ID" value="BRADI_2g31860v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K7Z6"/>
    <dbReference type="OrthoDB" id="443682at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K7Z6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007320">
        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12298:SF6">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12298">
        <property type="entry name" value="PCDC2 PROGRAMMED CELL DEATH PROTEIN 2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04194">
        <property type="entry name" value="PDCD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="188"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK06977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="421" mass="47756" checksum="DD7880F32EE1E845" modified="2016-01-20" version="1">MEPDTKKLQNLHIASHEDETKPVAIFESDYMDDEDEEDSESQVTLGFLQEPEEPLDWHLLLPQHFPDKAGGAPAWLDPVNLPSGKSSTCGFCGDPLRFVLQLNAPVKSKETAYHRTFFVFMCPSMSCLLRDQHEQGKGWAGNPRRSELRCSVKVFRCQLPKNNPFYPVEEPKGCIGTECEAGLHARLCDWCGTWKGEKLCSRCRKASYCSKKHQELHWCASHKNDCCQIPGSFDGSILPVMAGVGWPEFMMVDDEETYCFASCGGNSSKQLVVQGQSKTDDITLSLMDQFEADDDNRCWASFLDRISRNPRDRQQVLRYCGEENAKPLWAVSSGSLRSADIPSCIYCNGPLRYEFQVMPQLLHYFHVENERDSLDWATIVVYTCQESCDKNISYKEEFVCVQLSPDTKGTYRTTSSPADYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KR30</accession>
    <name>A0A0Q3KR30_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_1g10750v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13529.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KR30"/>
    <dbReference type="STRING" id="15368.A0A0Q3KR30"/>
    <dbReference type="EnsemblPlants" id="KQK13529">
        <property type="protein sequence ID" value="KQK13529"/>
        <property type="gene ID" value="BRADI_1g10750v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13529">
        <property type="protein sequence ID" value="KQK13529"/>
        <property type="gene ID" value="BRADI_1g10750v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3KR30"/>
    <dbReference type="OrthoDB" id="673363at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KR30">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
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        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF4">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="6">
        <location>
            <begin position="521"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="618"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="626"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK13529.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="699" mass="81366" checksum="E0E2FCB728DE4556" modified="2018-03-28" version="1" fragment="single">TTTIPDNDNTDEQFPEDEVLSQPHEPYLGMRFDTLSCAKDHYNAYALVNGFSIRSSTSRRSLYTNEVEKQLFVCNKFRKPKVDGKKAPKQRRRETIKGTNCKARLIVKLMDSRWQVVYFIAKHNHPLITRPSLTKYLRSHQGIPKEEENFLRILHDSNLETGRMMQLMSSFYGSSLLVPYTTKAISNYRSRMRTQTHGGDMAETISYFTVLLDEEQRVQNLLWVDGAARKAYEDAYSDCISFDTTYLTNQYNMPFASFIGINRHGQSFMLGCGFLRDEKATSFDWLFETYLDAMHGKAPQNIITDQDWAMRASIAKVFPNTVHRNCRWHIMKKAQEKLGSFLGRRPELVAQFNEVVDYSLTPEEFEQSWAQMIAVHDVASNKHIAWLYNIRATWVPCYFKDCFFPFLQSTQHSEGFNAVLKRYVNPHNSILNFVQQYEKIHLKILVKEGGNDYRTDHVQVRPWSSYPIERQALNVYTRDIYYRFREEFEKIGRYDVRQLANDIYELYPNRKYCCEYGTRTYKVTARVEEASYSCECCNFQKDGLLCCHILKIFTHCSVDEISQDYIIKRWKQQAIREEVAREEEKPDKVAREACGTDAGKQMVEKHVRAMRTELSHLNKTRKTPKKAATTVPTAKTSTTAPTQPVDASAVRNPDRSATKGKTRKRRFENALELRPKRRNKCALCGSEDHNQATCLSKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KS68</accession>
    <name>A0A0Q3KS68_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100840061</name>
        <name evidence="5" type="ORF">BRADI_1g13030v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559610.1">
        <property type="nucleotide sequence ID" value="XM_003559562.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KS68"/>
    <dbReference type="FunCoup" id="A0A0Q3KS68">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13873">
        <property type="protein sequence ID" value="KQK13873"/>
        <property type="gene ID" value="BRADI_1g13030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840061"/>
    <dbReference type="Gramene" id="KQK13873">
        <property type="protein sequence ID" value="KQK13873"/>
        <property type="gene ID" value="BRADI_1g13030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840061"/>
    <dbReference type="OrthoDB" id="687619at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KS68">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010584">
        <property type="term" value="P:pollen exine formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
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        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129421" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="4">
        <location>
            <begin position="33"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK13873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="228" mass="22699" checksum="841B0E177883387B" modified="2016-01-20" version="1">MERSHHLLLVLGLLAALLPAAAATFGTTQPEPGAPCEPTLLATQVSLFCAPDMPTAQCCEPVVASVDLGGGVPCLCRVAAEPQLVMAGLNATHLLTLYTSCGGLRPGGAHLAAACEGPAPPAAVVSAPPPSAAPRRKQPAHEAPPPPPSTEKPSPPPQQDNVTAHGKAIPTHAATSPLAPAASMIHMSPPPACNPCSGSAASSAEGPLLIAALLLVITAIIVGTLDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LJF7</accession>
    <name>A0A0Q3LJF7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g44941v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_4g44941v3"/>
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    </dbReference>
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        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="379" mass="41081" checksum="AF4C49305B30C07B" modified="2016-01-20" version="1">MATAGCPAWVLFASQGYFGDDSNETSATAFTRGGRTIKASFFPARPPHPSNLCVHCPGAATAEGAPRVLSVAGDLILFRVSINTDGRPLSQVQRDYFIYGRPRSGRSPQSAAPAPTSRTTRLPVKLPSGEYNLAGHLTTNVVTIGGERGTMGWVDLWRGIIFCDLLREPLTLRSIPLPLPMTQIIGRTHLGRGMNSRGIGFVNGAGAGCLKFVELQLDAVDVSDETGFPILLVKGWSVTTWSNAGMSSRYEDWQEDLPTPVQAYQIIPDDSAVFSHSQQQQKLGTGLRLVLPAQGGGYCCEKAATTTTHSLQKLLVSDPCASLDGDDVVYLIARPKLYYPKTWILALDMKKKMLQAVVPFGILEEPDPNVIYCTSTLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LKQ4</accession>
    <name>A0A0Q3LKQ4_BRADI</name>
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        </submittedName>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK23881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="96" mass="10840" checksum="0E3D8F9E7292FD0D" modified="2016-01-20" version="1">MNTQLERKTDELAGVRFELNLLRRRMEIEILQYESKVAILERGAHKMRTFRSLVSVLVLMLLVAPVNCSTIVNWALLVTVGAMYANQAAARPILLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="BRADI_2g25843v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3MQ19"/>
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            <end position="138"/>
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        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
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        </location>
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        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="185" mass="19060" checksum="39307CE29007735A" modified="2016-01-20" version="1">MPEPIPPLNRADEASIPSPKPAASAIASNGATINSVSSVVASYVWICPPGFFSRFPAASPRLPLRCLPLSRAGLRSGKSGGAGRACSGGGHGARGAQCGGQQGPRAQAASSGNDNRARRQGTTSGHAGSGGSGRGARWAWHEGRQGSTIAAGGLWAGTRKLELWPREECDLGEGDIVRRRDRLCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
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    <dbReference type="EMBL" id="CM000880">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010232569.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3N9Z1"/>
    <dbReference type="STRING" id="15368.A0A0Q3N9Z1"/>
    <dbReference type="EnsemblPlants" id="KQK13542">
        <property type="protein sequence ID" value="KQK13542"/>
        <property type="gene ID" value="BRADI_1g10850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828134"/>
    <dbReference type="Gramene" id="KQK13542">
        <property type="protein sequence ID" value="KQK13542"/>
        <property type="gene ID" value="BRADI_1g10850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828134"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000088">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049:SF163">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK13542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="464" mass="49719" checksum="705C283E07A4A60A" modified="2016-01-20" version="1">MDAAGSSSPMHIVIFPWLASGHLLPCLELAERLAARGHLVSFVSTPRNLARLPPVSPALAPLVDLVALPLPRVAGLPDGAESTADVPADKFDLHRQAFDGLAAPFAAFLDADVGKKKKPDWIVADFVHHWVAAAAQEREVPCAMLVPCAAAVAVLAGPPPESISNADERQVIVKVMDAAPRFEAEQAMEEFAAEDASGSSSGLSVLSRFYMTLKRCKVVALRSCPELEPDAFPLLTRLYGKPAVPLGLLPPPPNGTRSRGMDDEAIIRWLNAQPASSVVYVALGSEAPLRAELLRELAHGLELAGTRFLWALRKPVGVQDGDSVLPDGFVERTSRRGLVVARWVSQVSILAHGAVGAFLTHCGWGSVVEGLQFGRPLIMLPIAGDQGPNARLMEERKVGVSVPRDEKDGSFTRGGVAGAIRAVVVEEDGRLFAANAEKLREIVASRECHERCIDGFIQHLRCCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NC80</accession>
    <name>A0A0Q3NC80_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BRADI_1g17135v3</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NC80"/>
    <dbReference type="STRING" id="15368.A0A0Q3NC80"/>
    <dbReference type="EnsemblPlants" id="KQK14542">
        <property type="protein sequence ID" value="KQK14542"/>
        <property type="gene ID" value="BRADI_1g17135v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14542">
        <property type="protein sequence ID" value="KQK14542"/>
        <property type="gene ID" value="BRADI_1g17135v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NC80"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000479">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000177">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase At1g17230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000416">
        <property type="entry name" value="Probable leucine-rich repeat receptor-like protein kinase At5g63930"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF123">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="608"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="670"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="699"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KQK14542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="954" mass="105243" checksum="252EAD1877B5B5F8" modified="2016-01-20" version="1">MLHTNNLTGSIPSIFGNLSKLITLHLYGNKLHGWIPREVGYLVNLEELALENNTLTNIIPYSLGNLTKLTKLYLYNNQICGPIPHELGYLINLEEMALENNTLTGSIPYTLGNLTKLTTLNLFENQLSQDIPRELGNLVNLETLMIYGNTLTGSIPDSLGNLTKLSTLYLHHNQLSGHLPNDLGTLINLEDLRLSYNRLIGSIPNILGNLTKLTTLYLVSNQLSASIPKELGKLANLEGLILSENTLSGSIPNSLGNLTKLITLYLVQNQLSGSIPQEISKLMSLVELELSYNNLSGVLPSGLCAGGLLKNFTAAGNNLTGPLPSSLLSCTSLVRLRLDGNQLEGDIGEMEVYPDLVYIDISSNKLSGQLSHRWGECSKLTLLRASKNNIAGGIPPSIGKLSDLRKLDVSSNKLEGQMPREIGNISMLFKLVLCGNLLHGNIPQEIGSLTNLEHLDLSSNNLTGPIPRSIEHCLKLQFLKLNHNHLDGTIPMELGMLVDLQILVDLGDNLFDGTIPSQLSGLQKLEALNLSHNALSGSIPPSFQSMASLISMDVSYNKLEGPVPQSRLFEEAPIEWFVHNKQLCGVVKGLSLCEFTHSGGHKRNYKTLLLATIPVFVAFLVITLLVTWQCRKDKSKKASLDELQHTNSFSVWNFDGEDVYKNIVDATENFSDTYCIGIGGNGSVYKAQLPTGEMFAVKKIHVMEDDELFNREIHALVHIRHRNITKLFGFCSSAHGRFLVYEYMDRGSLATNLKSHETAVELDWMRRLNIVMDVAHALSYMHHDCFAPIVHRDITSNNILLDLEFKACISDFGIAKILDMNSSNCTSLAGTKGYLAPELAYTTRVTEKCDVYSFGVLVLELFMGHHPGEFLSSLSSTARKSVLLKHMLDTRLPIPEAAVPRQIFEVIMVAVRCIEANPLLRPAMQDAIKVLSMNGGPSDLDYLHTEIVIPACWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NGE8</accession>
    <name>A0A0Q3NGE8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100826087</name>
        <name evidence="3" type="ORF">BRADI_1g28825v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238905.1">
        <property type="nucleotide sequence ID" value="XM_010240603.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238909.1">
        <property type="nucleotide sequence ID" value="XM_010240607.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312896.1">
        <property type="nucleotide sequence ID" value="XM_024457128.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312897.1">
        <property type="nucleotide sequence ID" value="XM_024457129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NGE8"/>
    <dbReference type="STRING" id="15368.A0A0Q3NGE8"/>
    <dbReference type="EnsemblPlants" id="KQK16454">
        <property type="protein sequence ID" value="KQK16454"/>
        <property type="gene ID" value="BRADI_1g28825v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826087"/>
    <dbReference type="Gramene" id="KQK16454">
        <property type="protein sequence ID" value="KQK16454"/>
        <property type="gene ID" value="BRADI_1g28825v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4206278at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46325:SF20">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN RIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46325">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN RIC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="2">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK16454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="240" mass="25161" checksum="BDCAF09FDDE15339" modified="2016-01-20" version="1">MSTKMKKGILRPFRYFTNIMDTKEQEIQIGFPTDVKHVAHIGWDGPSTANKKVEEPGAPSWMKDYHSAPLDSASFRSDRGGSAAAAASNPWASQEIVVDGLGDTSFRDTKSDAAGGDSPPSPGARRSRRNRSRDSAATSSMDASAGGGAEPTEKKDKAKKGIRKNRKKDKEKQDKAAEEGGSTTCQDLPAVPKKSNRRKNKGSSEGSGGASAKDGGAGAAPEEGAAPLPQVGEEDKGGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PA64</accession>
    <name>A0A0Q3PA64_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_4g02360v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PA64"/>
    <dbReference type="STRING" id="15368.A0A0Q3PA64"/>
    <dbReference type="EnsemblPlants" id="KQJ85904">
        <property type="protein sequence ID" value="KQJ85904"/>
        <property type="gene ID" value="BRADI_4g02360v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ85905">
        <property type="protein sequence ID" value="KQJ85905"/>
        <property type="gene ID" value="BRADI_4g02360v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT62361">
        <property type="protein sequence ID" value="PNT62361"/>
        <property type="gene ID" value="BRADI_4g02360v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ85904">
        <property type="protein sequence ID" value="KQJ85904"/>
        <property type="gene ID" value="BRADI_4g02360v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ85905">
        <property type="protein sequence ID" value="KQJ85905"/>
        <property type="gene ID" value="BRADI_4g02360v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62361">
        <property type="protein sequence ID" value="PNT62361"/>
        <property type="gene ID" value="BRADI_4g02360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827430"/>
    <dbReference type="OrthoDB" id="442460at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3PA64">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070139">
        <property type="term" value="F:SUMO-specific endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.130">
        <property type="entry name" value="Ubiquitin-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46915">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46915:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="317"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="180"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ85905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="693" mass="78612" checksum="371D0242DF35938D" modified="2016-01-20" version="1">MARPTNQGGGGRIDIDWAEMFKDPNPDNVSFAAASPTPSAAAKRMRKREAAAFDDSPVPWAAAERKVKRMRTRQAALDVELSDDCLRREIEWMTDEELREQMAAIPRPPFSTLLGESRRRNRRLLPLLEAEARRRGIDPRMRSKVVADCNRGNARPQASIVYDFDTDDEQAEDVARKYNKSSPIRPTNENYGPLGVNTRRSVKQTGRRAPIPPDSMYSSKSSSTTSYGNKQRVRAIDPEEHDNKKCLPSKNNYTSYCTKRRKTPHQYTSPLHFQEVHDVVLLDDEDVQPEEPVDCVVSDKWIEKKIYYPSSDDPEAVELSGSDIKCLSPGVYLSSPVINFYILYIKRERFQIEDGRGRFHMFNTYFYSKLQEALSGKGEFLKLRRWWKGVNIFQRGYIIIPIHGTSHWSLVIICIPAKESNSGPIILHLDSLGMHPSAEIFETVGRYLEAEWSHLRKNPPSDISISEAIWEDLPRNIHKEKVEVPGQNNAYDCGIFMLYYIKQFIRQAPERFTRDNLGMFSRSWFRPEDASDLRKRIRELLLEQFESEMVDDAISEAATSDGSDEEGIIKEGQSEVVTTCDSSEMLVGGGDASTSDEDIVEVGSLEEAPSIRECVLSEAAMFSDGTKDDVDTVNLGSVSPKAKPWNEIFPSGGSENNEVFVHRAPSPDIYSDSDIEITGVRNRRHDRPTYCIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PUC4</accession>
    <name>A0A0Q3PUC4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_3g02427v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PUC4"/>
    <dbReference type="EnsemblPlants" id="KQJ93049">
        <property type="protein sequence ID" value="KQJ93049"/>
        <property type="gene ID" value="BRADI_3g02427v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93049">
        <property type="protein sequence ID" value="KQJ93049"/>
        <property type="gene ID" value="BRADI_3g02427v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3PUC4"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF471">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="5">
        <location>
            <begin position="29"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="238"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ93049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="43409" checksum="387EAAFE774B5297" modified="2016-01-20" version="1">MAFAGVSVIDDGKLCASTASPVDAGYDDSGYHLLVVQGYTRTTKDTPTGEYVESRPFKVGGVRFLLRCYPNGRNEDAKEFISVTLDFGEQDPGVVHLFPVSVRCAKSLIDQTAQQDVVRRDVLERSRHLKNDSFTIRCDVVVCRFTFVVEKRRAGTITTMPWPDMPDHFSNLLLTKKGSDITFEVGGKMIAAHRCVLGARSSVFMEQFFGAGVMNEGATATSVVKIDGIKETMHCPTSARTRWRKMKEKSKKETARGRLKQEKKKEKQMKRKQMKRKEKRTDEEERVKDVMWFLHMLEAADRYDLQGLKMSYEEALARSLCVSMVADIIVGAEQRRCQWLKEVCLQFINSHTSLPSVFTPEVLAMVIRTSSLSGLKELMSKFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QPZ7</accession>
    <name>A0A0Q3QPZ7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g08415v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3QPZ7"/>
    <dbReference type="EnsemblPlants" id="KQK03530">
        <property type="protein sequence ID" value="KQK03530"/>
        <property type="gene ID" value="BRADI_2g08415v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03530">
        <property type="protein sequence ID" value="KQK03530"/>
        <property type="gene ID" value="BRADI_2g08415v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3QPZ7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="233" mass="25259" checksum="0773E95140ED427A" modified="2016-01-20" version="1">MTRGSGSRPTTTGTVVAHADRTMEEVAEEAGEGFAAYCRRGKGRNRQAASVTLGGCSPSSMPGDGGPPRRLLALASTMAATQQWAALYPPPTCAFPNPQLRVAPTSSAPRTPPSSARRSSSSASTSTAPICPVSSLPDLAFLHLNSNRFYEVGALWRGWAWSRVAPAAVGEAGGRAEGGACRRWWGRLGKAPRRRRGGRRRAGLRWGRETHEKRDKERWAWTCQWIRFRGGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RAK5</accession>
    <name>A0A0Q3RAK5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="18">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LOC100825085</name>
        <name evidence="27" type="ORF">BRADI_2g51967v3</name>
    </gene>
    <organism evidence="27">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">Bd21</strain>
            <strain evidence="28">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Bd21</strain>
        </source>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="28">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="18"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564365.1">
        <property type="nucleotide sequence ID" value="XM_003564317.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RAK5"/>
    <dbReference type="EnsemblPlants" id="KQK10098">
        <property type="protein sequence ID" value="KQK10098"/>
        <property type="gene ID" value="BRADI_2g51967v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825085"/>
    <dbReference type="Gramene" id="KQK10098">
        <property type="protein sequence ID" value="KQK10098"/>
        <property type="gene ID" value="BRADI_2g51967v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825085"/>
    <dbReference type="OrthoDB" id="741567at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RAK5">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
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        <property type="entry name" value="receptor-like serine/threonine-protein kinase SD1-8"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Bulb-type_lectin_dom"/>
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        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
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    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="S_locus_glycoprot_dom"/>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="SRK-like_kinase"/>
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    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="B_lectin"/>
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        <property type="entry name" value="PAN_2"/>
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    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="B_lectin"/>
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        <property type="entry name" value="PAN_AP"/>
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        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
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    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="14" id="KW-0675">Receptor</keyword>
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        <location>
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    <feature type="domain" description="Apple" evidence="26">
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            <begin position="354"/>
            <end position="446"/>
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    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="538"/>
            <end position="825"/>
        </location>
    </feature>
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        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
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        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00048679"/>
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            <dbReference type="PIRNR" id="PIRNR000641"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
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            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50026"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="857" mass="93230" checksum="33A07159CE56799C" modified="2016-01-20" version="1">MASIDFYHHLLLSLALLLLLPTACQARDTVTPGRPLGANETLVSGGDASFVLGFFTPPGGNGTYLGVWYSKVSVRTVVWVANRERPIPGHVADNLGRATLSVSATGTLSIVNAAGNNNSRHVVVWSVTPASRLASPTAKILDNGNLVLADGNGVAAWQGFDHPTDTLLPDMKLGIDYVTGRNRTLTAWKSPSDPSPGPVVMAMDTSGDPQVFIWNGGEKVWRSGPWDGVQFTGVPDTVTYSGFTFSFVNDAREVTYSFHVHRESIISRLGLNSTGNYGLLQRSTWVESAGTWNLYWYAPKDQCDAVSPCGPNGVCDTNNLPVCSCLRGFSPRSPAAWALRDGRDGCVRTTPLDCRNGSTGAGDDGFVAVRHAKVPDTARSVVDRGLSLEQCREACLGNCSCTAYASANVVGGDRRGTGSGCVMWNSGLTDLRVYPDFGQDLFVRLAAADLGLSSKSRKGSTIIIIAVAASISALAFLLALAGFLVCARKKKRSRKTGSSKWSGSSRSNARRYEGSSHGEDLELPIFDLGTIAAATDGFSINNKLGEGGFGPVYKGKLEDGQEIAVKTLSKTSVQGLDEFKNEVMLIAKLQHRNLVRLLGYSISGQERILVYEYMENKSLDYFLFEKSNSILLDWQLRYRIVEGIARGLLYLHQDSRYRIIHRDMKASNVLLDKEMTPKISDFGLARMFGSEETEINTRKVVGTYGYMSPEYAMDGVFSVKSDVFSFGVLLLEIISGRKNRGVYSYSNHLNLLGHAWSLWNECKGIELADETMNGSFNSDEVLKCIRVGLLCVQENPDDRPLMSQVLLMLSATDPDTLPTPRQPGFAARRILTETDTTSSKPDCSIFDSSTVTILEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RMA4</accession>
    <name>A0A0Q3RMA4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g14093v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14058.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RMA4"/>
    <dbReference type="EnsemblPlants" id="KQK14058">
        <property type="protein sequence ID" value="KQK14058"/>
        <property type="gene ID" value="BRADI_1g14093v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14058">
        <property type="protein sequence ID" value="KQK14058"/>
        <property type="gene ID" value="BRADI_1g14093v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3RMA4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14058.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="211" mass="21604" checksum="9C56794FD5EF6767" modified="2018-03-28" version="2" fragment="single">ANSHARTLSSLPQPPGSFSLSLPPEHSPLPQTLSSFLPGAGHPFLAVGKDGKEELRGVLPASPAPVPPLVPPSLEDEGPSGRPLEQDLARPPPCFAPSAPATATPASTRLHLGWIRPPPSPISLCCRSKPEVSSGRPPISLFPSGTSLFYSRSSRRRWSGAHGGSGHGPSSSGYLSSAPAIGARGGDGQGALGRSTPKTSTSTSSSRSVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RQV9</accession>
    <name>A0A0Q3RQV9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LOC100833129</name>
        <name evidence="27" type="ORF">BRADI_1g22650v3</name>
    </gene>
    <organism evidence="27">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">Bd21</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Bd21</strain>
        </source>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="28">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754410.1">
        <property type="nucleotide sequence ID" value="XM_014898924.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RQV9"/>
    <dbReference type="FunCoup" id="A0A0Q3RQV9">
        <property type="interactions" value="760"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3RQV9"/>
    <dbReference type="EnsemblPlants" id="KQK15418">
        <property type="protein sequence ID" value="KQK15418"/>
        <property type="gene ID" value="BRADI_1g22650v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833129"/>
    <dbReference type="Gramene" id="KQK15418">
        <property type="protein sequence ID" value="KQK15418"/>
        <property type="gene ID" value="BRADI_1g22650v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833129"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RQV9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002215">
        <property type="term" value="P:defense response to nematode"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009825">
        <property type="term" value="P:multidimensional cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009845">
        <property type="term" value="P:seed germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000388">
        <property type="entry name" value="Leucine-rich repeat receptor-like tyrosine-protein kinase PXC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000691">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF89">
        <property type="entry name" value="OS06G0585982 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 25" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129562" description="non-specific serine/threonine protein kinase" evidence="25">
        <location>
            <begin position="24"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="726"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="834"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="758"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="781"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="862"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KQK15418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1126" mass="121396" checksum="8A6D5D23DDCB5BB0" modified="2016-01-20" version="1">MSPLLLELLCLLLVAGSVVLTGAQNGAGEDKAVLVELKRFLQTNNKVNRGDYDAWPETATSPCNWAGVRCDAAGRVASLNLSNSAISGPAFGNFSRLPALVSLDLSDNSITGFLPADDLNQCRGLTHLNLSHNLITGPLHIPGLTNLRTLDVSGNRLNGSVAGNFPSICAGELEQLDMSTNRFTGNITGMLDGCGNKLERVDLSSNNFTGELWPGVSRFSQFSAAENNLTGSIPSSTFQDGCRLQSLDLSANKLAGSFPDSIAKCQNLTYLSLWGNNFAGTIPAGIGELGVLETLILGKNRFDRRIPQALTNCTALQFLDMSNNSFGGDVQEIFGSFAPSLKYLVLHHNGYTGGIVASGVLRLPRLARLDLSFNDFTGYLPPEVAEMKSLKYLMLADNNFSGGIPTEYGRLAELQALDLSNNALSGGIPASVGNLTSLLWLMLAGNKLSGQIPREIGRCSSLLWLNLADNRLTGEIPPEMAEIGNNPGPTFAKNREDSSVLAGSGECQAMKRWIPASYPPFSFVYTVMTRENCRSIWDRILKGYGIFPICTNNSSSQVRTNSISGYVQLSRNMLSGQIPSRIGAMRNLSLLHLDGNGFTGRIPPEIGQLPLVILNVSRNNISGPIPSEVGQIRCLERMDLSFNNLSGELPASLGRLTELAMFNVSYNPLLHGYVPTAGQFGTFDEQSFIGIPNITLHRDRAVAGAGKQQQPMEDATRGRKMLPRTILAWFFFSLVVAFIAGSVVFIVTSLRARYPVDQDPDLEHPKCGGGGGNGKHKLFQTSSSSSSPLPSSGWSSSSATGCSSTSTEAAVKVFRLDKTTAFTYRDIVAATGDFSDGRVIGRGGHGVVYRGVLPDGRTVAVKRLSRCRNDVGEEDGDGEREFRAEMEVLAGRMGFTWPHPNLVTLYGWCLSGSAKILVYEYLEGGTLESLIFSDAGVKWARRKEVAVGVARALVFLHHECAPAVVHRDVKASNVLLDGEGRARVTDFGLARVVRPGDTHVSTVVAGTVGYVAPEYGQTWRATTKGDVYSFGVLLMELATRRRAVGYGEEDDECLVDWARRAAKEGWKGRQQQLVKAQAGGDRLATSGEVFWELLAIGLRCTADAPHERPDMPEVLAALLDVDADGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CG84</accession>
    <name>A0A2K2CG84_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g09405v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CG84"/>
    <dbReference type="EnsemblPlants" id="PNT61038">
        <property type="protein sequence ID" value="PNT61038"/>
        <property type="gene ID" value="BRADI_5g09405v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61038">
        <property type="protein sequence ID" value="PNT61038"/>
        <property type="gene ID" value="BRADI_5g09405v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CG84"/>
    <dbReference type="OrthoDB" id="695426at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CG84">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33207">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="145"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT61038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="301" mass="33060" checksum="8E8C11A69A0DB73F" modified="2018-03-28" version="1" fragment="single">PTQSPRSATPKSPLFLASPAKQALRGIRDMADIDEVDASADEVESPLFLASPDVRLQALRGIRDMADIDEVDASADEVAGDATSKSPLFLASPAVRLQALGAVGEMELNDEVDAAAHRVEGLSLSRTSAETASTRISVSSVLDNDDILREILLRLRFSICLVRAALVCKGWLLIISDPDFLHHFHNLHPPTLLGFYLVADRLLQPRFVSVEDHPPELGLILGRASTYFDTWVDVPVTIWDCRNGQLLIDVFDKLAVQSPLNPRYGIAIYPQSPLKVCLDPAFRYNVLEFCQKMEAEGCTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHA6</accession>
    <name>A0A2K2CHA6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Filament-like plant protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100829663</name>
        <name evidence="5" type="ORF">BRADI_5g15000v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FPP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHA6"/>
    <dbReference type="STRING" id="15368.A0A2K2CHA6"/>
    <dbReference type="EnsemblPlants" id="PNT61416">
        <property type="protein sequence ID" value="PNT61416"/>
        <property type="gene ID" value="BRADI_5g15000v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61416">
        <property type="protein sequence ID" value="PNT61416"/>
        <property type="gene ID" value="BRADI_5g15000v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1917992at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CHA6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008587">
        <property type="entry name" value="FPP_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31580">
        <property type="entry name" value="FILAMENT-LIKE PLANT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31580:SF7">
        <property type="entry name" value="OS04G0505000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05911">
        <property type="entry name" value="FPP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="748"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="66"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="254"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="748"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT61416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="837" mass="94133" checksum="133937DA7EC51C54" modified="2018-03-28" version="1">MPPPRRRGCRAGGGASRSQERAILDAVRQRELFAPAYVSPVHVGLRRWSACGNWPRQWGGGNERFVQALRKKMTEMEGALKSCTEQLLLGREENEHLIIEGANKISSEQKKAQDLQQKLEGANKRFAKVVTENYNLRNTVNSKDKIIRELIESKTHSDQRLTEATAKLEFMQKQCGSLQYEVRMVQKELEIRNKEREYDLKSIDAAQKQQQENVQKIATLEAECQRLRTMVQKRLPGPAALAKMKDEVERRGATSVQNGMRRTRTSTTLQPPLRAANQRHSVSEGYIVKLQEMDDENRHLRQLLARKESEIQSVQLLYADEACKLSVVQRQLKDLWSDHDMEENNHFEQFTSPSVPKPENIRTGKRLTSRSQSRRIAGSDMQLLVDLAEIEKLEMVSRPSSAPHQCDTDASDTHSQMILSEILGRDQIPPQDGFSYKYPEWIQDILKLIIHKHRANKISVDVILDEVICALRSEISAKESDVASLAYNQAEKDSMVATLIEKVSCMIERSTENNVISFGVFLHEKTEVTSQLEHLVHVCSDVLDGKVNLQKLIDEVCLTLEWAMDQCFSCVCGLDNVDSYTNKSDQNESARTLSMHGKQAMQSAKSEMVSRMQQNVQKELIGTIEGQIPGDTLENRSQIQLATCKLDEQELARQEQADNLQEKQSVYYELEIADADGSMENLLEEDGKQVTTNSAISAAAEKLAECQETMTNLTKQLQALQTPASADASGKGKSGTVPLSVPKVLAEHDAKSQDFRSPRSEEEGACKKEQGSHAATKMNLVHEQGADTGHKSSSNGLTRAVLPPVIPKSPRTSFCADMKKKKRRASLLSRLVFRKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CIK0</accession>
    <name>A0A2K2CIK0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-soluble NSF attachment protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N-ethylmaleimide-sensitive factor attachment protein gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_5g21937v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNAP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CIK0"/>
    <dbReference type="FunCoup" id="A0A2K2CIK0">
        <property type="interactions" value="2146"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CIK0"/>
    <dbReference type="EnsemblPlants" id="PNT61864">
        <property type="protein sequence ID" value="PNT61864"/>
        <property type="gene ID" value="BRADI_5g21937v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61864">
        <property type="protein sequence ID" value="PNT61864"/>
        <property type="gene ID" value="BRADI_5g21937v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CIK0"/>
    <dbReference type="OrthoDB" id="9984275at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CIK0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF2">
        <property type="entry name" value="GAMMA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT61864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="223" mass="24723" checksum="4CDEBE0B9A5DEE3C" modified="2018-03-28" version="1">MESAGALAKELGLWNEVSDFYRRASEFYRECGRSQPASDALAKGASALEDKAPEEAIKLYDEACALLEDDGKEQMAFDLYRSAASLYVKLEKYSDAAGFFLRLGSAADKCNAINSQCKGYLSAIIIYLYAHDFQQAQKCYNDCSEVQAFLNSDQNRCAMKLLSAYEEGDAEEVKRAAQSNVINHLDHVVIRLAKKLPTGDLQAIKKDLAADDDRDSLDENDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CM69</accession>
    <name>A0A2K2CM69_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g11694v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CM69"/>
    <dbReference type="FunCoup" id="A0A2K2CM69">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CM69"/>
    <dbReference type="EnsemblPlants" id="PNT63122">
        <property type="protein sequence ID" value="PNT63122"/>
        <property type="gene ID" value="BRADI_4g11694v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63122">
        <property type="protein sequence ID" value="PNT63122"/>
        <property type="gene ID" value="BRADI_4g11694v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CM69"/>
    <dbReference type="OrthoDB" id="692259at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CM69">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="11"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="258" mass="28206" checksum="941921443F2B601F" modified="2018-03-28" version="1">MAAEAAAGVPQLPEELIIWEILTRLPAKPVGRCRAVCRSWRRGLTSDPKFLLAHHRRQPSLPLLTTEDDHERRIDAMDHRTGEQLPVLRTDRVSAAAKNLDLDLDVLASCDGLLLVIAYGGLHICNPATRERAPVPLPQQVSCVAGLYAHRPSGSYRVLRQEGRGRPRRVPRAHRRVRRERAPVRRGASGAVGVRGHGAGDAHDGVPAAARPGGRQDLLAPGDAARRRQHRQGQQHAGVRHGGRVVPAPAVARRRPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CN42</accession>
    <name>A0A2K2CN42_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g15765v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CN42"/>
    <dbReference type="EnsemblPlants" id="PNT63439">
        <property type="protein sequence ID" value="PNT63439"/>
        <property type="gene ID" value="BRADI_4g15765v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63440">
        <property type="protein sequence ID" value="PNT63440"/>
        <property type="gene ID" value="BRADI_4g15765v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63439">
        <property type="protein sequence ID" value="PNT63439"/>
        <property type="gene ID" value="BRADI_4g15765v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63440">
        <property type="protein sequence ID" value="PNT63440"/>
        <property type="gene ID" value="BRADI_4g15765v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CN42"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014294237" evidence="2">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="61" mass="6617" checksum="7B5FA054002B3B86" modified="2018-03-28" version="1">MVCGKTLLSLVSLRVLLEVNKGNAYSTTALSDNRPDSSPSPSTRTDLWKSSKTSIAQLTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CNE9</accession>
    <name>A0A2K2CNE9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104584980</name>
        <name evidence="3" type="ORF">BRADI_4g17400v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310594.1">
        <property type="nucleotide sequence ID" value="XM_024454826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CNE9"/>
    <dbReference type="EnsemblPlants" id="PNT63567">
        <property type="protein sequence ID" value="PNT63567"/>
        <property type="gene ID" value="BRADI_4g17400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584980"/>
    <dbReference type="Gramene" id="PNT63567">
        <property type="protein sequence ID" value="PNT63567"/>
        <property type="gene ID" value="BRADI_4g17400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584980"/>
    <dbReference type="OrthoDB" id="671755at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011676">
        <property type="entry name" value="DUF1618"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074:SF139">
        <property type="entry name" value="OS09G0567000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07762">
        <property type="entry name" value="DUF1618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1618" evidence="2">
        <location>
            <begin position="105"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="347" mass="38548" checksum="27EC230879630631" modified="2018-03-28" version="1">MINERNHTSARCPVVTRRRSDGGKKVETMQVTFCLAPPPHISYFCVTYNSGEPTLLSREPTILAMEGNIVLIGLEHTRRIDDIRVGARSNPPLRHGHHLGGTIVWVDLWQRIILCDVHLPAPGEEEKESSNPVMLRCIQLPEPMQLNNGLPLRGSSSFFRDIAVVQGRIKFVDIQIHAIPGSLVPNGWTAVTWSMAVGDPGFVKDDEIHSHDVVNALEPYFFVAHPTLSSQDGDILFLMAKANLDDPQSWVIAVDMKKKKVERKAKFTTQRTSSVHFAYWRTNISSYLTPAEDSKGIMKRRGPVLMGSSRKKPPTTTCMSGHGGDTVEGEAEDSNNLPSTGDDMDME</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CNJ5</accession>
    <name>A0A2K2CNJ5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g18491v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CNJ5"/>
    <dbReference type="EnsemblPlants" id="PNT63621">
        <property type="protein sequence ID" value="PNT63621"/>
        <property type="gene ID" value="BRADI_4g18491v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63621">
        <property type="protein sequence ID" value="PNT63621"/>
        <property type="gene ID" value="BRADI_4g18491v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CNJ5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
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            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="163" mass="15776" checksum="23BFBE27D362D6E4" modified="2018-03-28" version="1" fragment="single">MRRRKGAGAGRGRAAGGARGRGGGARARGSGAGGGGGGARGRARGHGRAGSGGGAGGGHGSGAARAAAGAGEDGRRRGRRWAERGRRSKNKSVHRFGGSGGEKQGLRGDAQGFGFGGSCAKKLERNRGRLGVVRGRASRAGSLGKKGGGAHRSVGAGGWAGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CPB2</accession>
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        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g21862v3</name>
    </gene>
    <organism evidence="3">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CPB2"/>
    <dbReference type="STRING" id="15368.A0A2K2CPB2"/>
    <dbReference type="EnsemblPlants" id="PNT63865">
        <property type="protein sequence ID" value="PNT63865"/>
        <property type="gene ID" value="BRADI_4g21862v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63865">
        <property type="protein sequence ID" value="PNT63865"/>
        <property type="gene ID" value="BRADI_4g21862v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CPB2"/>
    <dbReference type="OrthoDB" id="639356at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF13">
        <property type="entry name" value="OS09G0290500 PROTEIN"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
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            <end position="551"/>
        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT63865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="567" mass="66436" checksum="1468D74ACA1E3F7B" modified="2018-03-28" version="1">MVDEYLDIVERTFGSENDGFEFYNSYALEKGFSVRKSYVEWDEANEEIILRKFVCSREGSREEKHMKREDRKRRPRNLTRVGCRAKSVIARVKETGHWFVKDFIDEHTHPLAPRDLACLLRSHRRISDEQKADIVEMEISRLRKHKIMDILVMQYGGYDEVGCTTRDIYNFCHLYKQETVAAGDAQMVICHMMERQERDPNFFFKYLVHGEGHLKGLFWADSQSRLDHEVFGDVVVFDSTYRTNKYNLPFVPFVGLNHHRSIVVFGCGIISHETSESYEWMLRTFSAAMAQKHLISVITDGDLAMQRAIRVVWPDTIHRLCVWHIQQNIVRHLGDALVKVEFRSFIYDRSPIEEHEKKWLDFLERTKVTSQESWLHQMYQMRKLWCAPYLVGHCFLGLSSNQRSESLNSVLHTHLDGSMTLFKMLEHYERCLSTRCLNESILDIVALQSVPFTEVDASSLEKHAAQVFTRAMFALVRWSTNAASNCTMFEILDADNLSTYVVAKKDRREKKFQVHCEVKEGSLDRIYCFCHKLECLGTPCSHILYVKTNKRLLFVKFSLLQNQIFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CPT5</accession>
    <name>A0A2K2CPT5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_4g23699v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="PNT64034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CPT5"/>
    <dbReference type="STRING" id="15368.A0A2K2CPT5"/>
    <dbReference type="EnsemblPlants" id="PNT64034">
        <property type="protein sequence ID" value="PNT64034"/>
        <property type="gene ID" value="BRADI_4g23699v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64034">
        <property type="protein sequence ID" value="PNT64034"/>
        <property type="gene ID" value="BRADI_4g23699v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CPT5"/>
    <dbReference type="OrthoDB" id="303876at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CPT5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF41">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="382"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="8">
        <location>
            <begin position="465"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT64034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="542" mass="57736" checksum="F3D9F0B4D6B238D2" modified="2018-03-28" version="1">MYAHMHVRTKPRCPLPFTRSLGRGNSCNQGKTRPPAYIFTAFVITPSSSGSDAGKKRPVSDDDGRMSSELYLAVCQGRKEEAMALLLQQGSGIYQVSAERNTVLHLAAEQGHDELIQELYASCGDNILLSSQNSALETPLHRAARARHDRAVSLIVQLIWDSGDQSIIGCKNKVGDMALHLASRLGHGMAMEAMVSVAPRLASEVNDAGVSPLYLAAMSGSVPSVRAITTMCSDASAVGPNSQNALHAAIFRGSEMVSLLLDWKPSGQSLSYQADSSGSSPLHLALSDGDQAVVGAILNISPPCVVRMQDSGGLSALHIAAAMGHAHIVEALIKACPDITELRDNHGGNFLHSAARGGQSKVVQLVLRKRTLHSLLNVQDGDGNTPLHLAVAACAPSIVEALMRHGKVQADFMNNDGHTPLDLAARSTSFFSMLGLVVTLVAFRAQSHPQRQDRVKQWSSHDIKKRIENTLDSLAVVAVLIATVAFTAANSMPGSYEQADGTAPDRYGNMALRRVMEEPRSGAALYMGVADQYVNGLLRGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CTJ1</accession>
    <name>A0A2K2CTJ1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g41035v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CTJ1"/>
    <dbReference type="EnsemblPlants" id="PNT65351">
        <property type="protein sequence ID" value="PNT65351"/>
        <property type="gene ID" value="BRADI_4g41035v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65351">
        <property type="protein sequence ID" value="PNT65351"/>
        <property type="gene ID" value="BRADI_4g41035v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CTJ1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT65351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="70" mass="7947" checksum="48E4F897607E19B8" modified="2018-03-28" version="1">MASVHQACLSLCPCSSTVSFHSFRLVHIICTAGDNIDPLHHSPDHLDAIPRRRRRPPHTTWYRCSGCRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CV19</accession>
    <name>A0A2K2CV19_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g03831v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CV19"/>
    <dbReference type="EnsemblPlants" id="PNT65873">
        <property type="protein sequence ID" value="PNT65873"/>
        <property type="gene ID" value="BRADI_3g03831v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65873">
        <property type="protein sequence ID" value="PNT65873"/>
        <property type="gene ID" value="BRADI_3g03831v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CV19"/>
    <dbReference type="OrthoDB" id="1932629at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CV19">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT65873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="140" mass="16216" checksum="11390EF5B860F396" modified="2018-03-28" version="1">MEEEHKREIEQLKHANEMSCNTLESKLSCALVQQATDEAVINQLKSDLGAHKAQVDMLNSRLEQDLNDVIMLEQEEKNGMQRKLKKAEDELRVLKMKQAEQQRDSVSVQHVETLKQKVMKLRKENESLKRRLASSELDCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CWI0</accession>
    <name>A0A2K2CWI0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_3g10950v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CWI0"/>
    <dbReference type="STRING" id="15368.A0A2K2CWI0"/>
    <dbReference type="EnsemblPlants" id="PNT66390">
        <property type="protein sequence ID" value="PNT66390"/>
        <property type="gene ID" value="BRADI_3g10950v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66390">
        <property type="protein sequence ID" value="PNT66390"/>
        <property type="gene ID" value="BRADI_3g10950v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CWI0"/>
    <dbReference type="OrthoDB" id="2309723at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CWI0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000037">
        <property type="entry name" value="Glutathione S-transferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000198">
        <property type="entry name" value="Glutathione S-transferase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01148">
        <property type="entry name" value="Xi_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="314"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT66390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="470" mass="53086" checksum="2766FBAF19090F94" modified="2018-03-28" version="1">MVRSSLPSQRHAELNSQVSESAIRREAHSHLPPSCCQANARHCAHHAKTYSTQQPPLQNHEIPRSHLRPFPTFRATPVLLATGASHLAAFPPRFRALQHCYNNKLPPPPLWVGMLGLVPSSSSYLLYRRLAAVRTFSAAAGSVNMAARSALDEVTDSGAFDRSPSTFRNFVSRDSSARFPAVPGRYHLYVSYACPWASRCLAYLKLKGLDNAISFTSVKPIFERTRETDDHLGWVFPATGDEDPGADPDPFNGVKTVRELYEIASTSYTGKPTVPVLWDKQLKTVVNNESSEIIRMFNSEFNEIAENPGLDLNPPHLQASIDEVNDLVYDAINNGVYKCGFAKKQEPYDEAVRNLYEALDKCEEILSKQRYMCGNQLTEADIRLFVTLIRFDEVYAVHFKCNKKLLREYPNLFNYTKDIYQIPGISSTVNMEHIRKHYYGSHPSINPYRIVPAGPNIDYNATHDREKYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D039</accession>
    <name>A0A2K2D039_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g30095v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D039"/>
    <dbReference type="EnsemblPlants" id="PNT67646">
        <property type="protein sequence ID" value="PNT67646"/>
        <property type="gene ID" value="BRADI_3g30095v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67646">
        <property type="protein sequence ID" value="PNT67646"/>
        <property type="gene ID" value="BRADI_3g30095v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D039"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35218:SF11">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35218">
        <property type="entry name" value="RNASE H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT67646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="8016" checksum="A4CA78C4CDF9C98C" modified="2018-03-28" version="1">MNILSWNYLRIGNDVTVRELRTLVRRFAPSILCLQETQVANTRAQNLSFTLDFQNCFAVSSSGRSGGLAMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D1C2</accession>
    <name>A0A2K2D1C2_BRADI</name>
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            <fullName evidence="4">Dipeptidylpeptidase IV N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g35670v3</name>
    </gene>
    <organism evidence="1">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D1C2"/>
    <dbReference type="EnsemblPlants" id="PNT68080">
        <property type="protein sequence ID" value="PNT68080"/>
        <property type="gene ID" value="BRADI_3g35670v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68080">
        <property type="protein sequence ID" value="PNT68080"/>
        <property type="gene ID" value="BRADI_3g35670v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D1C2"/>
    <dbReference type="OrthoDB" id="43744at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D1C2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="WD40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32161">
        <property type="entry name" value="DPP6 N-TERMINAL DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32161:SF26">
        <property type="entry name" value="OS03G0403400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT68080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="748" mass="82344" checksum="FF967E40EF842314" modified="2018-03-28" version="1">MAGESRGNIAFFATYRPPVPLDIFSSPVPPSSSRDEVHLTDGVSYNYDCRPIPASALKALLRRPKLAADGGATEADVDAGRVSGLVFVSERDGGLETLRIALRFDKDNKTKVFSLADVFGSGDFSGTRLEDSGCFGGGYKVGSRTVDHSLIYVSTKEPVKDRRSPWTVVYKTNLRTGKTERLTPKGAYDLSPAVSPSGKKVAVASFRAEGWQGEIENLKTDIFVMNVEKPPLGRKLLVKDGGWPSWGSENFVFFHRGSDKTLPSGMIETSWAVFRLDVATGETVRVTPEGLDAFTPAAISETKVAVATIRQKSKFSDVRVEAQYRHIEIFDVAAGGAQAQQITQKTRPKGDHYNPFVLDGGARVGYHRVRSDLLKPGDDVPRNFHKLESPLKDVGLFRVSGVFPTISKDGSKLAFVDNEFKAVWVADAQGLRIVYETKGPDSIFSTMWNQNPDKDVLYVCMGPSFNAGKPLEICAIPNVSSGARQRRLLTKGKFNNAFPSSSPDGTRFVFRSTRDHAKSERKHKNLYIMEDQEVGEYGEGGKVTRLTDGEWTDTHCQWSPSGDWIVFSSTRDKPPTAAPLDHGLDPGYFAVFMASDPAVVIRVVGSGDDLSGHVNHPVFSPNGRSIAVTSDLAAVSADPISLPLFLHSVRPYGDIFAVDIDDILRLGDDAEKLKKIKDLRRKFHRITHSRYEYSTPEWTMFATDDPNAQWNVLVKKDAAYTPACPYAYPDGGESWHMTGHLCIPKRCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D1J9</accession>
    <name>A0A2K2D1J9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g36725v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D1J9"/>
    <dbReference type="STRING" id="15368.A0A2K2D1J9"/>
    <dbReference type="EnsemblPlants" id="PNT68167">
        <property type="protein sequence ID" value="PNT68167"/>
        <property type="gene ID" value="BRADI_3g36725v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68167">
        <property type="protein sequence ID" value="PNT68167"/>
        <property type="gene ID" value="BRADI_3g36725v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D1J9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D1J9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044176">
        <property type="entry name" value="TRL4_chloroplastic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47912">
        <property type="entry name" value="THIOREDOXIN-LIKE 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47912:SF1">
        <property type="entry name" value="THIOREDOXIN-LIKE 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="365"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT68167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="41258" checksum="3F91F025D7770CD9" modified="2018-03-28" version="1">MENSSFAHGRAQQRFGGELTRYPCNRGMESMRLLSRAMTGGARTGNMTILPAVEASHRVSALGHAMIRRKTPQTFVPESLPVTLDGSLRRRWSLHGNNRSRDQTPRLQLSPPTLGFDNLPRRPPLVNSRLGGRGRQRRVSSSRAIHRSSCEAHRRGGVTCASRTGWEMETVMADRAASTVRGPDSGMGAAQSTWGVANEEARGSTATGDGRSTSSSAERGGSSSPKEPQSSFSGESSSGSENSQGLEEQRMEGDGEAAVDLGATQPPDSLIGQIWSKPTAVEEGDDVEAVSVGGDGGRGEEAGNLEENDEVYPVECVTAFKTGEEFTYFLERSRATGTLLVVDFFRSSCGSCNYVEQGFMKLSSAPATTVTPSSPSSTSAQRLVGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D3D1</accession>
    <name>A0A2K2D3D1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g45162v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D3D1"/>
    <dbReference type="EnsemblPlants" id="PNT68775">
        <property type="protein sequence ID" value="PNT68775"/>
        <property type="gene ID" value="BRADI_3g45162v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68775">
        <property type="protein sequence ID" value="PNT68775"/>
        <property type="gene ID" value="BRADI_3g45162v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D3D1"/>
    <dbReference type="OrthoDB" id="786283at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT68775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11544" checksum="82EA9B01C1421234" modified="2018-03-28" version="1">MTTPSPKILVWNAHGLNSRAKRCAVFQVVSNAGASIICLQESKLQVVIACIVTKCLGVILASNAAVVSLSNPHISDHTSTARVTTVDGCWWLTEAYGPHRVANKDAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D3N9</accession>
    <name>A0A2K2D3N9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100840938</name>
        <name evidence="8" type="ORF">BRADI_3g46900v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572651.2">
        <property type="nucleotide sequence ID" value="XM_003572603.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235593.1">
        <property type="nucleotide sequence ID" value="XM_010237291.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D3N9"/>
    <dbReference type="STRING" id="15368.A0A2K2D3N9"/>
    <dbReference type="EnsemblPlants" id="PNT68893">
        <property type="protein sequence ID" value="PNT68893"/>
        <property type="gene ID" value="BRADI_3g46900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840938"/>
    <dbReference type="Gramene" id="PNT68893">
        <property type="protein sequence ID" value="PNT68893"/>
        <property type="gene ID" value="BRADI_3g46900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840938"/>
    <dbReference type="OrthoDB" id="1424968at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D3N9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000006">
        <property type="entry name" value="NAC domain-containing protein 100-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF12">
        <property type="entry name" value="OS02G0579000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="16"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="305"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT68893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="345" mass="37299" checksum="94298514AF920087" modified="2018-03-28" version="1">MEHDVHQQQLEEPMELPPGFRFHPTDEELVTHYLARKTADPTGFAARAVGEADLNKCEPWDLPSRATMGEKEWYFFVVKDRKYPTGTRTNRATESGYWKATGKDREILRGKALVGMKKTLVFYTGRAPKGGKTGWVMHEYRLEGKHAPAPYSLPRAATSKQDEWVLCRVFKKSLEPSAAGSNNGARKGACTGMAADAVGSSMSLADAGLAALPPLIDVAASGSFAPPPAAHVTCFSNALEGQFLNPPFLLPATTAAAAHSAGENIHIAMESAASPFFASMYTQDVSGGMAHEFLLDGGGGGWYSKGERERLSGASQDTGLTSEVNPTEISSSRQHMDHEPTFWGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D657</accession>
    <name>A0A2K2D657_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_3g60981v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D657"/>
    <dbReference type="FunCoup" id="A0A2K2D657">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69756">
        <property type="protein sequence ID" value="PNT69756"/>
        <property type="gene ID" value="BRADI_3g60981v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69756">
        <property type="protein sequence ID" value="PNT69756"/>
        <property type="gene ID" value="BRADI_3g60981v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D657"/>
    <dbReference type="OrthoDB" id="682957at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D657">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1116">
        <property type="entry name" value="OS12G0273300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="165"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="290"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="499"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="623"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT69756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="989" mass="112076" checksum="BAE5725184F42EC4" modified="2018-03-28" version="1">MGKKKRQQQKRTNENVTGGQQPKEAAVPAKRATNKNVTGQQQPNDPAPQGHTAKNKNNNEEEVPGTSTQRKPTDPNLEVTEAPKEEVCPLTLSTTNPFSEFSFGATTKAAPKNETPPPIFGIPYFVPQTTTPEPLEFSFGATATVEKKNSNQGKEDTVGEVPPDQLDMNVRVWAGKVQDLSYDMEDIVNTFMVQVENGHENEQLTNMKNRAKKLLKKTIKLFNKGKALHQISVAIQEAQDLTKELSDLRKRYKLMIHSNNTGAANTGANIDPRLLDMYKDVTELIGIEKERDELIQSLTEDGYKSKQNLKTISIVGFGGLGKTTLAKAVYNKIKVEFDCGAFVSVSRTPDVKRILKDILYDLDKDKFQNIHSTTRGENLLIDELREFLEDKRYLIMIDDIWDVKTWGIIKCALCGNSLGSRIITTTHNVNVSEACHSSEEYFLVIITVASLLSSNQNRMSKDHWCHVLKSIWRGITEDAIVGYYDLPSHLKTCLLYLSIFPEDYKIERHRLIWRWIAEGFIHHGKQGKGLFEVGESYFNELVNRSMIQPADMNYEGKAYFCRVHDLVLDLICSLSREENFVAIWDANGQSIHESQSNFRRLSLQNISMAELGTPQLGTMNMSQVRSFSLFMVDINPMPSLSCFQVLRVLDLEGCHSVRRKDKIYLRHLGSVLHLKYLGLNRTHVSELPIEIGNLQILQTLDIRRTYVRELPSSVFQLRKLMRLCINSEIKLPAASLGNLVSLQELSTLNVDNFSGEELKELGSLTELRFLMICFSCKMDENQDKALADLEFFGVGCTKFIPVRRVPRPTLHRFDEDLLSAMPSSSLPFLSYVHISVDQVRGDHIVTLGMLPVLRFLYLDARRSKKELAELERGFVLSADGAFPCVTECTFLGFQTFPCMFPQGTMPMLRSFHFSFLARQIFSHHFELSMSHLPLIQQVTADVWCHGANAGEVKFAETEFRRAAADHPNLPTCLIDRYGIDRLVNSCNHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D6J6</accession>
    <name>A0A2K2D6J6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g02622v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT69900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D6J6"/>
    <dbReference type="EnsemblPlants" id="PNT69900">
        <property type="protein sequence ID" value="PNT69900"/>
        <property type="gene ID" value="BRADI_2g02622v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69900">
        <property type="protein sequence ID" value="PNT69900"/>
        <property type="gene ID" value="BRADI_2g02622v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D6J6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT69900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="69" mass="7431" checksum="142619F951269C13" modified="2018-03-28" version="1">MCLPWCRPRQHDYGRNRKDSRPTASSQLGHDGAGDGQKAPPTPPHLQPPAAGGGHGYAQDKVDDETTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DFP8</accession>
    <name>A0A2K2DFP8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100835835</name>
        <name evidence="3" type="ORF">BRADI_2g53440v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232492.1">
        <property type="nucleotide sequence ID" value="XM_010234190.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DFP8"/>
    <dbReference type="EnsemblPlants" id="PNT73118">
        <property type="protein sequence ID" value="PNT73118"/>
        <property type="gene ID" value="BRADI_2g53440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835835"/>
    <dbReference type="Gramene" id="PNT73118">
        <property type="protein sequence ID" value="PNT73118"/>
        <property type="gene ID" value="BRADI_2g53440v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1930285at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DFP8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900150">
        <property type="term" value="P:regulation of defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040244">
        <property type="entry name" value="EDR4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021480">
        <property type="entry name" value="Zinc_ribbon_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31105">
        <property type="entry name" value="EXTRA-LARGE G-PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31105:SF27">
        <property type="entry name" value="ZINC-RIBBON DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11331">
        <property type="entry name" value="Zn_ribbon_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Probable zinc-ribbon" evidence="2">
        <location>
            <begin position="374"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT73118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="498" mass="53075" checksum="CFAC489D36C00039" modified="2018-03-28" version="1">MYYSEHSAIKRSLPAAGEGTNTIKPGKHMQSEQVKMRFGRCPYCRAMVYQDPKAVVYLCSKCRTPIRGKEPEPTDENEYALAQLEILSFDTMSVFSDELEPPSSDFDPTSRETVAASASSSSQFRPYGVIRTGPRSGDLDRDEEPGSGRRNGGSPMHNRVGELRPASRRTRRPSSADLDAPRPAEESEFDVPRTRSASCYQRRASPLSSQELEAAMDPAAAGLAGSPLTDPSFQQDLLQALENLRKLIVAVEEPFRASTGAPRFGSANASAPGHRQTASCNNDSAAQKVTRRDSRILRRLESQLAQALPTNSPRHSQDATTSSSSSSVSASASALSGSRRGASISAPASASASALSSSRRGASTSRNLLCRPILGGTPFVACEKCSELLQLPSALSVKKSAILQCGGCGEELAVKLPARGGPGSSASTDGPRKIFSAPQPAGFGADDAAAEEYMRASARSRLSGEQLRQGPDEGPLHRVLGYSSVSSVLRSRRYSDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DJH5</accession>
    <name>A0A2K2DJH5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g14572v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
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        <property type="protein sequence ID" value="PNT74430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DJH5"/>
    <dbReference type="EnsemblPlants" id="PNT74430">
        <property type="protein sequence ID" value="PNT74430"/>
        <property type="gene ID" value="BRADI_1g14572v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74430">
        <property type="protein sequence ID" value="PNT74430"/>
        <property type="gene ID" value="BRADI_1g14572v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DJH5"/>
    <dbReference type="OrthoDB" id="498125at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF19">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT74430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15468" checksum="7D77AD031795D235" modified="2018-03-28" version="1" fragment="single">DWKILTWNIRGLNGQDKWVQVQRKIKESGCSIICLQETKRTVVDNHFIHNFCPKRFNKFDYLPSNGRFSETPVDVTDFSLSVEFKYTTSNKTWILTNIYGPCSDDRKVQFAHWLQNNIIPDHVEGLIVGDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKA0</accession>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g20765v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DKA0"/>
    <dbReference type="EnsemblPlants" id="PNT74709">
        <property type="protein sequence ID" value="PNT74709"/>
        <property type="gene ID" value="BRADI_1g20765v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74709">
        <property type="protein sequence ID" value="PNT74709"/>
        <property type="gene ID" value="BRADI_1g20765v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DKA0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT74709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="104" mass="11523" checksum="EC86BD586F33DBE9" modified="2018-03-28" version="1">MTRCPPDGRRGCLMRSPAKNVLINPSPHACVYLKPRRRMPSCTQAGEEDMPLHVQDRSICGGGQYVLQWRKRRLPTDDQPATSATCPATTSPADLCLCVIYHDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DMR4</accession>
    <name>A0A2K2DMR4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g34961v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DMR4"/>
    <dbReference type="EnsemblPlants" id="PNT75569">
        <property type="protein sequence ID" value="PNT75569"/>
        <property type="gene ID" value="BRADI_1g34961v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75569">
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        <property type="gene ID" value="BRADI_1g34961v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DMR4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="196" mass="21144" checksum="F7B0BF9573F19CAB" modified="2018-03-28" version="1">MWDAETLVGGATCKRMSEGDATCKKRTWRRCCILGRERHRHAGCARCCDQLIAIYSFFSFILSIFSLLLLLLRPAAAALAAWAAGSPRVTSPCVLLLGAVFVGTCRSTSRACGWRGDYNNDAMRMGLAMVRGPVMERVEELLMPLYFASSGLKTDIATFFSGGGRGARGGGAGHRDVVRIEARNVRGGGAGHRPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DP41</accession>
    <name>A0A2K2DP41_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g43423v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DP41"/>
    <dbReference type="EnsemblPlants" id="PNT76050">
        <property type="protein sequence ID" value="PNT76050"/>
        <property type="gene ID" value="BRADI_1g43423v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76050">
        <property type="protein sequence ID" value="PNT76050"/>
        <property type="gene ID" value="BRADI_1g43423v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DP41"/>
    <dbReference type="OrthoDB" id="695705at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35046:SF24">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35046">
        <property type="entry name" value="ZINC KNUCKLE (CCHC-TYPE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="80"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT76050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="556" mass="64512" checksum="14FB5CC9086EA318" modified="2018-03-28" version="1">MESRPMLSSDRHERFSPYPSSSSLVDIGKAKVYHNPCNANNCFGASFDGNNDAKAYIYWEIALIEQFTCFHVLDSRKVQIASSKFRFDALSWWSELVREKKIPHTWIDMKKIMREKYAPPSYAYELRSKLRRLNQDDKTVDDYYLEFQILTLRSGLDETEGRKMDRFYYGLNFEISYMIEYGQYDSIFCLLNLAREVERKLQRRSTTNESSPQGNDTKDCVAELNFPCDQRVELSSEFSAPCDIDDAPTILIAPPVPISFYNDRSAQINVKDNESCEEIDILSGETCDVDKFGLGCVELITHGVIDKDPPINSLCYITLDTPMYLSHAMDKVSELRISKSFTVHGYTIHLIGQHDVNNNILVHEICITCDNFPVLGEKKFLHMLNHFNMTSNICVHYLPNSCLHDCMNISALTCSKFQHLVVTNLGTINYYSPVLGWYNGEQCKPNTRHCFTYICKKTFCQFSCWNVIHDDCLTNYLMRFCYKTLIVQERRCIKMDDIYIYHAHTLSILSVSFQHKRRRGRLSFQEREDDMDMTMDTTITNLIIGVFNNNSNQASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DV25</accession>
    <name>A0A2K2DV25_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g74087v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DV25"/>
    <dbReference type="EnsemblPlants" id="PNT78127">
        <property type="protein sequence ID" value="PNT78127"/>
        <property type="gene ID" value="BRADI_1g74087v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78127">
        <property type="protein sequence ID" value="PNT78127"/>
        <property type="gene ID" value="BRADI_1g74087v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DV25"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DV25">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000558">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g39710"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051222">
        <property type="entry name" value="PPR/CCM1_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47942:SF89">
        <property type="entry name" value="OS03G0165100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47942">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="50"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="85"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="120"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="190"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="260"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="330"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="365"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="400"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT78127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="49714" checksum="E9A1F5CC09FA8827" modified="2018-03-28" version="1">MALIDSMVSKGIKPGLVTYNSVLKGLLRNGRWDKAREVFRAMDACGVAPDVRSFNMLIGGFCRAGELEEALRFYKEMRGRRVTPDVVSFSCLIGLFTRRGEMDHAAEYLREMREFGLMPDGVIYTMVIGGFCRAGLMLEALRVRDEMVAFGCLPDVVTYNTLLNGLCKERRLSDAEELLNEMKERGVPPDLCTFTTLIHGYCRDGNIEKALQFFDTISDQRLRPDIVTYNTLIDGMCRQGDLGKANELWDDMHSREIFPNHVTYSILIDSHCEKGQVDNAFAFLDEMVNKGIVPNIMTYNSIIKGYCRSGNVSKGQQFLPKMRHDKVMPDLITYNTLIHGYVKEGKMHEAFNLLKIMENENVQPDAVTYNMIISGFSVHGNMQEADWVYKKMGARGIEPDRYTYMSMINGHVVAGNSKKSFQLHDEMLQKGLAPDDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNW8</accession>
    <name>I1GNW8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calcium load-activated calcium channel</fullName>
            <shortName evidence="14">CLAC channel</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100822805</name>
        <name evidence="17" type="ORF">BRADI_1g10416v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Calcium-selective channel required to prevent calcium stores from overfilling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TMCO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560514.1">
        <property type="nucleotide sequence ID" value="XM_003560466.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312977.1">
        <property type="nucleotide sequence ID" value="XM_024457209.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312978.1">
        <property type="nucleotide sequence ID" value="XM_024457210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNW8"/>
    <dbReference type="STRING" id="15368.I1GNW8"/>
    <dbReference type="EnsemblPlants" id="PNT74218">
        <property type="protein sequence ID" value="PNT74218"/>
        <property type="gene ID" value="BRADI_1g10416v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74219">
        <property type="protein sequence ID" value="PNT74219"/>
        <property type="gene ID" value="BRADI_1g10416v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74220">
        <property type="protein sequence ID" value="PNT74220"/>
        <property type="gene ID" value="BRADI_1g10416v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74221">
        <property type="protein sequence ID" value="PNT74221"/>
        <property type="gene ID" value="BRADI_1g10416v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822805"/>
    <dbReference type="Gramene" id="PNT74218">
        <property type="protein sequence ID" value="PNT74218"/>
        <property type="gene ID" value="BRADI_1g10416v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74219">
        <property type="protein sequence ID" value="PNT74219"/>
        <property type="gene ID" value="BRADI_1g10416v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74220">
        <property type="protein sequence ID" value="PNT74220"/>
        <property type="gene ID" value="BRADI_1g10416v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74221">
        <property type="protein sequence ID" value="PNT74221"/>
        <property type="gene ID" value="BRADI_1g10416v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822805"/>
    <dbReference type="eggNOG" id="KOG3312">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081121_0_0_1"/>
    <dbReference type="OMA" id="GMFGDFK"/>
    <dbReference type="OrthoDB" id="342726at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008559">
        <property type="entry name" value="TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917:SF0">
        <property type="entry name" value="CALCIUM LOAD-ACTIVATED CALCIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917">
        <property type="entry name" value="PNAS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023322">
        <property type="entry name" value="DUF841_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 14" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="55"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR023322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="PNT74221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="201" mass="21215" checksum="E58A23F9061CC5F7" modified="2012-06-13" version="1">MASALSSLRYGDSLSVVAISGATAVLCEAISWLLIYRTATYNSLRLSIERHSRKLDSMKSTSSGGEGASSQPAGSSSSSRAKKMDRVETSLKDAARELSLAKLKSGAVVAAVLFVVFGLLNSLFEGRAVAKLPFAPVPLVQRMSHRGLPGTDPTDCAMVFLYFLCSMSIRTNLQKLLGFTPPRAAAAAGGGLFPMPDPKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQQ9</accession>
    <name>I1GQQ9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100833769</name>
        <name evidence="11" type="ORF">BRADI_1g16010v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562378.1">
        <property type="nucleotide sequence ID" value="XM_003562330.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQQ9"/>
    <dbReference type="STRING" id="15368.I1GQQ9"/>
    <dbReference type="EnsemblPlants" id="KQK14407">
        <property type="protein sequence ID" value="KQK14407"/>
        <property type="gene ID" value="BRADI_1g16010v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833769"/>
    <dbReference type="Gramene" id="KQK14407">
        <property type="protein sequence ID" value="KQK14407"/>
        <property type="gene ID" value="BRADI_1g16010v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833769"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059471_1_2_1"/>
    <dbReference type="OMA" id="TWCPLPI"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044653">
        <property type="entry name" value="AZF1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45988">
        <property type="entry name" value="C2H2 TYPE ZINC FINGER TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45988:SF16">
        <property type="entry name" value="ZINC FINGER PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094022" description="C2H2-type domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK14407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="190" mass="19994" checksum="6E850B7CD7F562FB" modified="2012-06-13" version="1">MVVAALELSLSLSLPTPSAAPALSKDDYLAICLAALANTRGHVASKWCPPAPAAEELRFRCMVCGKAFASYQALGGHKSSHRKSPPAASAAADNSQSYETSSGGSSGPHQCTICGRGFSTGQALGGHKRCHYWDGTSVSVSANSASGVTTRRNLFDLNLLPVPESVGIKRWAREEEEEVQSPLPAKKLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1GS50</accession>
    <name>I1GS50_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Histone acetyltransferase</fullName>
            <ecNumber evidence="3 17">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100839490</name>
        <name evidence="20" type="ORF">BRADI_1g20770v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">Bd21</strain>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 17"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562650.1">
        <property type="nucleotide sequence ID" value="XM_003562602.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GS50"/>
    <dbReference type="STRING" id="15368.I1GS50"/>
    <dbReference type="EnsemblPlants" id="KQK15117">
        <property type="protein sequence ID" value="KQK15117"/>
        <property type="gene ID" value="BRADI_1g20770v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839490"/>
    <dbReference type="Gramene" id="KQK15117">
        <property type="protein sequence ID" value="KQK15117"/>
        <property type="gene ID" value="BRADI_1g20770v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839490"/>
    <dbReference type="eggNOG" id="KOG2747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011815_2_1_1"/>
    <dbReference type="OMA" id="DSPEGNN"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GS50">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010224">
        <property type="term" value="P:response to UV-B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18642">
        <property type="entry name" value="CBD_MOF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000022">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000067">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025995">
        <property type="entry name" value="Tudor-knot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11717">
        <property type="entry name" value="Tudor-knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="19">
        <location>
            <begin position="159"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="16">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KQK15117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="435" mass="50178" checksum="3D3E3B5D583F1382" modified="2012-06-13" version="1">MGSMELSTAPENGTAAACNGTAAPPNGGVERRLRSSSTSAAWGSHLPLEVGTRVMCRWRDQKPHPVKVIERRKSSSYSSPADYEYYVHYTEFNRRLDEWVKLEQLDLETVEADVDEKVEDKATSLKMTRHQKRKIDETHVEQGHEELDAASLREHEEFTKVKNIAKIELGKYEIDTWYFSPFPPEYNDSAKLFFCEFCLNFMKRKEQLQRHMKKCDLKHPPGDEIYRCGTLSMFEVDGKKNKVYGQNLCYLAKLFLDHKTLYYDVDLFLFYILCECDDRGCHMVGYFSKEKHSEESYNLACILTLPPYQRKGYGKFLIAFSYELSKKEGKVGTPERPLSDLGLLSYRGYWTRVLLEILKKHKSNISIKELSDMTAIKADDILSTLQSLDLIQYRKGQHVICADPKVLDRHLKAAGRGGLDVDVSKLIWTPYKEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSH4</accession>
    <name>I1GSH4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL6 alpha-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100834080</name>
        <name evidence="5" type="ORF">BRADI_1g21850v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562717.1">
        <property type="nucleotide sequence ID" value="XM_003562669.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSH4"/>
    <dbReference type="FunCoup" id="I1GSH4">
        <property type="interactions" value="1781"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GSH4"/>
    <dbReference type="EnsemblPlants" id="KQK15313">
        <property type="protein sequence ID" value="KQK15313"/>
        <property type="gene ID" value="BRADI_1g21850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834080"/>
    <dbReference type="Gramene" id="KQK15313">
        <property type="protein sequence ID" value="KQK15313"/>
        <property type="gene ID" value="BRADI_1g21850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834080"/>
    <dbReference type="eggNOG" id="KOG3255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_0_0_1"/>
    <dbReference type="OMA" id="CASHITN"/>
    <dbReference type="OrthoDB" id="1872507at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000003">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="12"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="101"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK15313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="189" mass="21307" checksum="52BE340EF88A3132" modified="2012-06-13" version="1">MKTILASETMEIPEEVTVKVAAKMITVSGPRGTLTRNFKHLNLDFQLQEGGRKLKVDAWFGTRRTMAAIRTAISHVQNLITGVTKGFRYKMRFVYAHFPINASITSGNKAIEIRNFLGEKKVRKVDMLDGVTILRSEKVKDELVLDGNDIELVSRSAALINQKCHVKKKDIRKFLDGIYVSDKGAIKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSZ5</accession>
    <name>I1GSZ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100843214</name>
        <name evidence="3" type="ORF">BRADI_1g23390v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_010238057.3"/>
    </dbReference>
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    <dbReference type="FunCoup" id="I1GSZ5">
        <property type="interactions" value="3"/>
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    <dbReference type="EnsemblPlants" id="KQK15514">
        <property type="protein sequence ID" value="KQK15514"/>
        <property type="gene ID" value="BRADI_1g23390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843214"/>
    <dbReference type="Gramene" id="KQK15514">
        <property type="protein sequence ID" value="KQK15514"/>
        <property type="gene ID" value="BRADI_1g23390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843214"/>
    <dbReference type="eggNOG" id="ENOG502RZQ2">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="entry name" value="OS01G0532300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS01G0532300 PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK15514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24006" checksum="D40F8A1965219C3F" modified="2012-06-13" version="1">MEQKQIVRTFSALGQLAKIAAMLLLFLIVPFVSPSLKGPYLYLVFNALVVSLGVQAGIISVSVSVPSNQNKSPSASNLTNPGETADHVTNSDHRHLVSGGTKQRAPLLLVERREVNKLAHRAAIKEGVVGVAKKLTEKINNCPSRASIFFIRSVDADYAGEVVEASSLTVHQVAEERKEWKADDAGVRLSKQELFTNAEAFIGNFYKQLKMQRDESCVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTC3</accession>
    <name>I1GTC3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100834687</name>
        <name evidence="8" type="ORF">BRADI_1g24390v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562891.1">
        <property type="nucleotide sequence ID" value="XM_003562843.4"/>
    </dbReference>
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    <dbReference type="FunCoup" id="I1GTC3">
        <property type="interactions" value="892"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GTC3"/>
    <dbReference type="EnsemblPlants" id="KQK15688">
        <property type="protein sequence ID" value="KQK15688"/>
        <property type="gene ID" value="BRADI_1g24390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834687"/>
    <dbReference type="Gramene" id="KQK15688">
        <property type="protein sequence ID" value="KQK15688"/>
        <property type="gene ID" value="BRADI_1g24390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834687"/>
    <dbReference type="eggNOG" id="ENOG502QRBC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035664_8_1_1"/>
    <dbReference type="OMA" id="HHAIMGA"/>
    <dbReference type="OrthoDB" id="1921961at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GTC3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048653">
        <property type="term" value="P:anther development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009555">
        <property type="term" value="P:pollen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="NAC transcription factor 56"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF248">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAC"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="30"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
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        <location>
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            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK15688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="435" mass="46590" checksum="0072D9475053AAF2" modified="2012-06-13" version="1">MRSMGSPDSSSGSAPPQHQQQQRAGSAPELPPGFRFHPTDEELVVHYLKKKAAKAPLPVTIIAEVDLYKFDPWELPEKATFGEQEWYFFSPRDRKYPNGARPNRAATSGYWKATGTDKPIMASGCAREKVGVKKALVFYRGKPPKGLKTNWIMHEYRLADSSSSGATASRPPPVVAGGSGGRAASLRLDDWVLCRIYKKINKAAAGDQQRSMECEDSVEDAVAAYHPSSYAMAGMVGAGAHGGSNNNYTSLLHHHHHHHDSHEDNFLDGLLTVTEDHAGLSAGGTSLQSHLAAASRASLPTAATKQLLAPPSSSATPFNRLHASTAVGILPQARNFPGFNRSRNNNTVGSMSLSSTTTAAAMDDNGRGNNNAVNTMPAFMNALPVVQDASTYHQQHVILGTTSLPVPPESTVSGAASAFQQQQHPVQISGVNWNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTM3</accession>
    <name>I1GTM3_BRADI</name>
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        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g25200v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GTM3"/>
    <dbReference type="FunCoup" id="I1GTM3">
        <property type="interactions" value="475"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GTM3"/>
    <dbReference type="EnsemblPlants" id="KQK15838">
        <property type="protein sequence ID" value="KQK15838"/>
        <property type="gene ID" value="BRADI_1g25200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15838">
        <property type="protein sequence ID" value="KQK15838"/>
        <property type="gene ID" value="BRADI_1g25200v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825797"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_28_4_1"/>
    <dbReference type="OMA" id="AFLRYAY"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="RNA recognition motif-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48029">
        <property type="entry name" value="NUCLEOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48029:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="40"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK15838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="138" mass="14780" checksum="5B4B5AAAF7BCB634" modified="2012-06-13" version="1">MAAVASRAGAARAGFRRMFSVSAFSPPPPPAARPAAEPCNNLFVSGLNKRTTTDGLREAFSKFGQVTEARVITDRISGYSRGFGFVKYATVEEAGEGIKGMDGKFLDGWVIFAEYAKQREAQQPPQSAGTPSGYQYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXC9</accession>
    <name>I1GXC9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100831413</name>
        <name evidence="12" type="ORF">BRADI_1g36160v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563633.1">
        <property type="nucleotide sequence ID" value="XM_003563585.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXC9"/>
    <dbReference type="EnsemblPlants" id="KQK17695">
        <property type="protein sequence ID" value="KQK17695"/>
        <property type="gene ID" value="BRADI_1g36160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831413"/>
    <dbReference type="Gramene" id="KQK17695">
        <property type="protein sequence ID" value="KQK17695"/>
        <property type="gene ID" value="BRADI_1g36160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831413"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005738_5_2_1"/>
    <dbReference type="OMA" id="CLRSCYK"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000013">
        <property type="entry name" value="Aspartyl protease AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000021">
        <property type="entry name" value="Aspartyl protease AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF902">
        <property type="entry name" value="OS06G0610800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094123" description="Peptidase A1 domain-containing protein" evidence="10">
        <location>
            <begin position="25"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="11">
        <location>
            <begin position="163"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="54"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="111"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK17695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="503" mass="51356" checksum="4E8E3A1956C92F00" modified="2012-06-13" version="1">MATAGSASSSRCCLLLLMMHLVIAAATLVGAAAPAAGSSADRVLLRVDSLFPGPSSCTSTQERKPITATSSAARVPIVHRHGPCSPLAGAHAGKPPSHAEILAADQNRVESLHHRVSSTTTGLGGKPRTKKKTPGHSSVPASSSSSSSSVPASSGLSLGTANYVVPIGLGTPPSRFTVVFDTGSDTTWVQCRPCVVSCYKQKDRLFDPAKSSTYANVSCADPACADLDASGCNAGHCLYGIQYGDGSYTVGFFAKDTLAVAQDAIKGFKFGCGEKNRGLFGQTAGLLGLGRGPTSITVQAYEKYGGSFSYCLPASSAATGYLEFGPLSPSSSGSNAKTTPMLTDKGPTFYYVGLTGIRVGGKQLGAIPESVFSNSGTLVDSGTVITRLPDTAYAALSSAFAAAMAASGYKKAAAYSILDTCYDFTGLSQVSLPTVSLVFQGGACLDLDASGIVYAISQSQVCLGFASNGDDESVGIVGNTQQRTYGVLYDVSKKVVGFAPGAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1H081</accession>
    <name>I1H081_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UDP-glucuronate 4-epimerase</fullName>
            <ecNumber evidence="9">5.1.3.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100846585</name>
        <name evidence="12" type="ORF">BRADI_1g47000v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>UDP-alpha-D-glucuronate = UDP-alpha-D-galacturonate</text>
            <dbReference type="Rhea" id="RHEA:11404"/>
            <dbReference type="ChEBI" id="CHEBI:57635"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="EC" id="5.1.3.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.6" evidence="9"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564175.1">
        <property type="nucleotide sequence ID" value="XM_003564127.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H081"/>
    <dbReference type="STRING" id="15368.I1H081"/>
    <dbReference type="EnsemblPlants" id="KQK19214">
        <property type="protein sequence ID" value="KQK19214"/>
        <property type="gene ID" value="BRADI_1g47000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846585"/>
    <dbReference type="Gramene" id="KQK19214">
        <property type="protein sequence ID" value="KQK19214"/>
        <property type="gene ID" value="BRADI_1g47000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846585"/>
    <dbReference type="eggNOG" id="KOG1371">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_2_1"/>
    <dbReference type="OMA" id="MENPISY"/>
    <dbReference type="OrthoDB" id="202470at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050378">
        <property type="term" value="F:UDP-glucuronate 4-epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033481">
        <property type="term" value="P:galacturonate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000198">
        <property type="entry name" value="UDP-glucuronate 4-epimerase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43574">
        <property type="entry name" value="EPIMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01713">
        <property type="entry name" value="NUCEPIMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="11">
        <location>
            <begin position="100"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK19214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="441" mass="48465" checksum="A3C34297D8AC3A94" modified="2012-06-13" version="1">MRGVLEEDMLPSTPGKVKIERAGAMSRQLHRCFASTGTMFLWALFLVAMTATYLSFHSFVDTSSRYFAASWGGLHWERQIRASASPRRPPGSAEGAGLSVLVTGAAGFVGTHCSLALRKRGDGVVGIDNFNKYYDPSLKKARRALLASHGVFVVEGDINDGRLLAKLFDVVPFTHVLHLAAQAGVRYAMENPSSYVHSNIAGLVSLLEACKDADPQPAVVWASSSSVYGLNDLVPFSEAHRTDRPASLYAATKKAGEEITHTYNHIYGLSVTGLRFFTVYGPWGRPDMAYFSFTRNILQGKPITVYRGKDHVDLARDFTYIDDIVRGCLASLDTAGRSTGTGGKKRGPAPYRIFNLGNTAPVTVPTLVSILERYLRVEAKKNVVEMPGNGDVPFTHANISLAREQLGYKPTTTLEMGLKKFVRWYLSYYGYNRGTHTFSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1H212</accession>
    <name>I1H212_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Membrane protein of ER body-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100820938</name>
        <name evidence="3" type="ORF">BRADI_1g52150v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228138.1">
        <property type="nucleotide sequence ID" value="XM_010229836.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H212"/>
    <dbReference type="FunCoup" id="I1H212">
        <property type="interactions" value="942"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H212"/>
    <dbReference type="EnsemblPlants" id="KQK20053">
        <property type="protein sequence ID" value="KQK20053"/>
        <property type="gene ID" value="BRADI_1g52150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100820938"/>
    <dbReference type="Gramene" id="KQK20053">
        <property type="protein sequence ID" value="KQK20053"/>
        <property type="gene ID" value="BRADI_1g52150v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ85">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_349649_0_0_1"/>
    <dbReference type="OMA" id="NQDANMK"/>
    <dbReference type="OrthoDB" id="1924921at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H212">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010168">
        <property type="term" value="C:ER body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052843">
        <property type="entry name" value="ER_body_metal_sequester"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38937">
        <property type="entry name" value="MEMBRANE PROTEIN OF ER BODY-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38937:SF2">
        <property type="entry name" value="MEMBRANE PROTEIN OF ER BODY-LIKE PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="658"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="708"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="742"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="773"/>
            <end position="799"/>
        </location>
    </feature>
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        <location>
            <begin position="173"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="362"/>
        </location>
    </feature>
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        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
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        <location>
            <begin position="458"/>
            <end position="515"/>
        </location>
    </feature>
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        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="475"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK20053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="835" mass="91371" checksum="521F373606C50F94" modified="2012-06-13" version="1">MMEVVVESKKPWDQFKEDVEIMEEETAAGTLLTRETKKKLLDFGANGNGAHVNGEEEEEEELKAEEVLEAEDQQQQQQHKSIFFDPTKGLWKCRHCDWTYCLTSPYENNTLNHRGYRHHIEWNRESLVHNKSFYASQNKVPEIITEAMRFGAQNEDADATPTPKGKELEAHENGNAQEINDTPNNGKLENGSHSNGSHQVSNGSDTKAVHMFERIGRSEINPTEAEIEAHENGTGQEVIHPPNNYKLEHESYSSDSHQVSNGSETKIAYVFETRREQIYPTEADKGEHVTEAKPHIEEYDLEKIIDQQETHDLYCPNCNSCITRRVILKKRKRTPRIPDEPTKKPHTEEQPSTAAPERDGQESPEVFRCLSCFSFFIPTGCGLNIFRIFGGRDLHQQADVQQPSAPEQMPQSENCASWLLSCFQPGDGPNQPYADAGSATTPLLPDKQSLSGTITTAAASTSVHSHGSIGKPDQSTESSSQVQTTTTTTITTTTATESTSSTTTSEAASSSSHTTAATGFLQTEVIEAMTGQILPPKPAGVAVTDATHLSGKEGTGTTIFENSSLPSPAIPTSGFREIPGVIPRGDKLIPQAADPPRHLVLPVSDAPTTENHPRVPTSGQRDEWDILKSIVYGGLVESVTSLSIVSAAAASGAKTLDIFILGIANLIGGIPLIFHNISDLRNLGDVNGNDEQVGHYWLQLGRRSKFRLHMFLALLSYIMFGLLPPVLYGLSFRKSDDRENKMMTVAAASLACVALLALGKAHVSRSRTYFKTLMYYLMIVVSASGLSYVAGVLITRLLVHFGIIDQGASAPPAPPSLSSSFPYAVGAETSAWASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2R9</accession>
    <name>I1H2R9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC112270121</name>
        <name evidence="1" type="ORF">BRADI_1g54420v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313602.1">
        <property type="nucleotide sequence ID" value="XM_024457834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2R9"/>
    <dbReference type="EnsemblPlants" id="KQK20421">
        <property type="protein sequence ID" value="KQK20421"/>
        <property type="gene ID" value="BRADI_1g54420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112270121"/>
    <dbReference type="Gramene" id="KQK20421">
        <property type="protein sequence ID" value="KQK20421"/>
        <property type="gene ID" value="BRADI_1g54420v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R3JS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129534_5_0_1"/>
    <dbReference type="OMA" id="CAHSERR"/>
    <dbReference type="OrthoDB" id="661559at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33181">
        <property type="entry name" value="OS01G0778500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33181:SF52">
        <property type="entry name" value="OS07G0204400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK20421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7822" checksum="479FA1E4BCD83957" modified="2012-06-13" version="1">MGWLHSLFSPIRRLWVRAHSRRRNRRGMHILYKDVQSCQDEDVQVLWSIFIDSHRHPALMKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1H455</accession>
    <name>I1H455_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100837955</name>
        <name evidence="3" type="ORF">BRADI_1g58680v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561471.2">
        <property type="nucleotide sequence ID" value="XM_003561423.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753825.1">
        <property type="nucleotide sequence ID" value="XM_014898339.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H455"/>
    <dbReference type="EnsemblPlants" id="KQK21091">
        <property type="protein sequence ID" value="KQK21091"/>
        <property type="gene ID" value="BRADI_1g58680v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK21092">
        <property type="protein sequence ID" value="KQK21092"/>
        <property type="gene ID" value="BRADI_1g58680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837955"/>
    <dbReference type="Gramene" id="KQK21091">
        <property type="protein sequence ID" value="KQK21091"/>
        <property type="gene ID" value="BRADI_1g58680v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21092">
        <property type="protein sequence ID" value="KQK21092"/>
        <property type="gene ID" value="BRADI_1g58680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837955"/>
    <dbReference type="eggNOG" id="ENOG502R3DQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011465_2_0_1"/>
    <dbReference type="OMA" id="NFWASPD"/>
    <dbReference type="OrthoDB" id="668319at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046527">
        <property type="entry name" value="PIR2-like_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33120">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33120:SF16">
        <property type="entry name" value="PIR2-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12274">
        <property type="entry name" value="DUF3615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20235">
        <property type="entry name" value="PIR2-like_helical"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIR2-like helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="PIR2-like helical" evidence="2">
        <location>
            <begin position="258"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3615" evidence="1">
        <location>
            <begin position="479"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK21092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="665" mass="74325" checksum="4B7CBB020DA3B565" modified="2012-06-13" version="1">MAGGGGPNTWRGKVLDAIYGHYEDALAALPPALAPRLLAAGVSFGFADPVTNIITNTVVCSDPKPKKRKRPATAREVLSKVVAAGNGPSPPESRTIAERSLVGLLTFVTSYFRHLSSWDALHYLYLSRADLLVAVHLVEVDRCYYRRRKDKFCIRSLAVKTALKCAALSASLPNIDEFLAGSYACAFHLKLITQIAPPAKRCRRLLSGLLQKPPVKLNKSNNDPIDLAVLRLHHHVPDASIQKVPSELTDSLRGVLMDRIHKQYLKAVSQLATKEFRISHHRSLFKAGYCYGPFSSPVSNIIVNTIWYGTAFPALQDFEVDMISTLSHVEFRSLDGLISFMRVSIPEVSDHDAMVYLLKSNLNIRKAIRMATQGGHDTSGRHESGYKAAADVSYHPKPEAYLEFVVHFLPTVRIAVRSLLQDSQTPSSSKVLDLSRLLSPLGAKSLEPIDDELTKNALERLSNFKTGFLTQQSFVCGKVKAVLRKYEQTVGCCYELCIICGVNGSVGKSTGIRHLKFQYTHANFWAMPKNGNSAMLFFAEFSNDEDSKNLRSFCHPVSDLSRHGRCCYCEFQGIRIVHPIEICWEGANDFEKMARGEHKITNELITSSGKVEDYMVGIYKEDYIYMDPTQDAKLIRIMNRATYMMSLDRVDEKGRLRELDPQQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1H5J3</accession>
    <name>I1H5J3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100839631</name>
        <name evidence="12" type="ORF">BRADI_1g62630v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in sequestration of excess metal in the cytoplasm into vacuoles to maintain metal homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557927.1">
        <property type="nucleotide sequence ID" value="XM_003557879.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H5J3"/>
    <dbReference type="STRING" id="15368.I1H5J3"/>
    <dbReference type="EnsemblPlants" id="KQK21720">
        <property type="protein sequence ID" value="KQK21720"/>
        <property type="gene ID" value="BRADI_1g62630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839631"/>
    <dbReference type="Gramene" id="KQK21720">
        <property type="protein sequence ID" value="KQK21720"/>
        <property type="gene ID" value="BRADI_1g62630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839631"/>
    <dbReference type="eggNOG" id="KOG1485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="RNSEAIP"/>
    <dbReference type="OrthoDB" id="435980at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H5J3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000023">
        <property type="entry name" value="Metal tolerance protein C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1350:FF:000008">
        <property type="entry name" value="Metal tolerance protein C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF15">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="72"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="11">
        <location>
            <begin position="294"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK21720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="498" mass="53181" checksum="AAD30272F153C41C" modified="2012-06-13" version="1">MGFRFARLAAGVARATASHRGPHPSSTSARRVLAPLASSGEPSGGYRFVPVRGHGGHSHHHGEASEKIFRLGLASDVVLTVGKAITGYLSGSTAITADAAHSLSDIVLSTVALLSYKAAKAPRDKDHPYGHGKFESLGALGISSMLLVTAGGIAWHSFEVLQGVMSSAPDIIGSTSHINHNHGSGGHNHGIDLEHPVLALTVTTLAISIKEGLYWITKRAGEKEGSGLMKANAWHHRADAISSVVALVGVGGSILGVPLLDPLAGLVVSGMILKAGVQTGYESILELVDAAVDPSLLEPIKETIVKVDGVKGCHRLRGRKAGTSLYLDVHIEVYPFLSVSAAHDIGETVRHHIQKAHNQVAEVFIHIDPSYSVGTDMDKENTLENLERRNSDDIPRQQNAEAIVSDIVLSHFSKKMSLEHLMLHYLQGRVLLQVQVSMPPEILIRDAMDIAKQAEEEILNADASISQVSIQLRLGQQIQKLHLATSKTGAKDLQAEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1H613</accession>
    <name>I1H613_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100843904</name>
        <name evidence="7" type="ORF">BRADI_1g63927v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558027.1">
        <property type="nucleotide sequence ID" value="XM_003557979.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H613"/>
    <dbReference type="STRING" id="15368.I1H613"/>
    <dbReference type="EnsemblPlants" id="KQK21916">
        <property type="protein sequence ID" value="KQK21916"/>
        <property type="gene ID" value="BRADI_1g63927v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843904"/>
    <dbReference type="Gramene" id="KQK21916">
        <property type="protein sequence ID" value="KQK21916"/>
        <property type="gene ID" value="BRADI_1g63927v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843904"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001724_0_0_1"/>
    <dbReference type="OMA" id="WLVHFHV"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000065">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000078">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1161">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK21916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="480" mass="52087" checksum="17F41D34AF1652F9" modified="2012-06-13" version="1">MAAPEKKPCHVVLVPFPAHGHVAPHMQLARLLHARGIHVTLVHTELHYRRLVQANNGTVATTVNIPGFGVEVIPDGLSLEAPPQTLAAHLEALEQNCFEPFRELLRALEDPDDVPRLSCVIADAPMSFASLAARDVGVPDVQFFTASACGLMGHLQFEELIKRGLVPLKGSSYKTDGTFDATLDWVPGMKGMRLKDMPTFCHTTDADNALLRIHVRQMHVVATSKAIILNTFHDYEKDVVDALAALLPRIYTVGPLSSIMAASLTAAPTSNGGDFSGLTDTAPTSLLQEDTGCIKWLDGKEARSVVYVSYGSHAAMSSEKIKEFASGLESCGYPYLWVLRPDMAADVEVGKNGLVVPWCAQEAVLAHPAVGLFVTHCGWNSILETVMAGVPVLGWPMMSEQTTNCRQVSMSWKIGTELPQEARGHEIAALVREMMVGKKGLEARETTLKWKRLAEDATKEGGSSYGNLGSFVEDVLHEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAC9</accession>
    <name>I1HAC9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100840862</name>
        <name evidence="6" type="ORF">BRADI_1g77010v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558940.1">
        <property type="nucleotide sequence ID" value="XM_003558892.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAC9"/>
    <dbReference type="FunCoup" id="I1HAC9">
        <property type="interactions" value="2813"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HAC9"/>
    <dbReference type="EnsemblPlants" id="KQK23919">
        <property type="protein sequence ID" value="KQK23919"/>
        <property type="gene ID" value="BRADI_1g77010v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840862"/>
    <dbReference type="Gramene" id="KQK23919">
        <property type="protein sequence ID" value="KQK23919"/>
        <property type="gene ID" value="BRADI_1g77010v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840862"/>
    <dbReference type="eggNOG" id="KOG0424">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_12_1_1"/>
    <dbReference type="OMA" id="WKPTISI"/>
    <dbReference type="OrthoDB" id="6600758at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061656">
        <property type="term" value="F:SUMO conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000038">
        <property type="entry name" value="SUMO-conjugating enzyme SCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="5"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK23919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="160" mass="18030" checksum="BA68E7C483EC7F95" modified="2012-06-13" version="1">MSGGIARGRLAEERKAWRKNHPHGFVAKPETLGDGTVNLMVWHCTIPGKQGTDWEGGYFPLTLNFSEDYPSKPPKCKFPQAFFHPNVYPSGTVCLSILNEDSGWRPAITVKQILVGIQDLLDQPNPADPAQTDGYHLFIQDPTEYKRRIRLQAKQYPPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I1HC78</accession>
    <name>I1HC78_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1664 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836333</name>
        <name evidence="3" type="ORF">BRADI_2g03750v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568710.1">
        <property type="nucleotide sequence ID" value="XM_003568662.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HC78"/>
    <dbReference type="EnsemblPlants" id="KQK02797">
        <property type="protein sequence ID" value="KQK02797"/>
        <property type="gene ID" value="BRADI_2g03750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836333"/>
    <dbReference type="Gramene" id="KQK02797">
        <property type="protein sequence ID" value="KQK02797"/>
        <property type="gene ID" value="BRADI_2g03750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836333"/>
    <dbReference type="eggNOG" id="ENOG502QTG9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056257_2_0_1"/>
    <dbReference type="OMA" id="GAFKVNH"/>
    <dbReference type="OrthoDB" id="544175at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012458">
        <property type="entry name" value="DUF1664"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47289">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE (DUF1664)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47289:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE (DUF1664)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07889">
        <property type="entry name" value="DUF1664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1664" evidence="2">
        <location>
            <begin position="93"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK02797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="348" mass="37227" checksum="8D6B9BAFFF1B4000" modified="2012-06-13" version="1">MVLGKIALVIGSGIVGTLVTGGGDAKLPDLRDVLSVSFKFMSKQDKKDGTSTSSPHTDQLLSQVNHLREQLRIIVDKKQVHIINVNGTPGAGAYGLTAIVIGAIGYLYIRWKGWKISDLMFVTKRGLSDACNVVGKQLDQVSESVNASKKHLSGRIDRVDCSLDECHEIIEATGKEVTVIHGDLSAFQQEIQSVHLVVRTLETKLGRLAYTQDHTTRGIHELCEFTKRLDRSPKADTLQISSTPLPAIESAEGITRAASLPPGLEPDSPVAQSPRAEAHKVVRSSTTISASGLSMLAETSIPKRGVIGRATSMKEGSSEVTNGTSSSATLKNPVSSSSRFGFLRGFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1HHT8</accession>
    <name>I1HHT8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100845078</name>
        <name evidence="6" type="ORF">BRADI_2g20347v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568148.1">
        <property type="nucleotide sequence ID" value="XM_003568100.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HHT8"/>
    <dbReference type="FunCoup" id="I1HHT8">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HHT8"/>
    <dbReference type="EnsemblPlants" id="KQK05494">
        <property type="protein sequence ID" value="KQK05494"/>
        <property type="gene ID" value="BRADI_2g20347v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845078"/>
    <dbReference type="Gramene" id="KQK05494">
        <property type="protein sequence ID" value="KQK05494"/>
        <property type="gene ID" value="BRADI_2g20347v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845078"/>
    <dbReference type="eggNOG" id="KOG1171">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065905_0_0_1"/>
    <dbReference type="OMA" id="EAMEDAH"/>
    <dbReference type="OrthoDB" id="6283463at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044522">
        <property type="entry name" value="TSO1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46159:SF2">
        <property type="entry name" value="OS05G0509400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46159">
        <property type="entry name" value="PROTEIN TESMIN/TSO1-LIKE CXC 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="87"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="243"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="278"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK05494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="377" mass="41612" checksum="070709EC7847C33A" modified="2012-06-13" version="1">MEATPISMKPPSPAPAPAPAQPPPPPPPPPPPLIEAVPLAIVEPLPSPSPPPAVAGGLEADPSAANQLALATTPKRQRVEEAADGNGCKHCACKKSKCLKLYCPCFAGGGYCSEKCGCVPCFNKSDYAETVQTTRKVLLSRQKRMSLKINRRSEANAEAMEDAHNSSSSTPPRRGCNCKKSSCLKKYCDCYQDGTGCSLFCRCEDCKNPYGKNEGIMTEETKRFIYTGADLDNSEDDHEFVVERSPRMQSPISKESSLQHTPSRNKASSRDTHLFPQGLSQWQPRWQSSKRQINDRAIDDSGENRNLNNDWQLAKHQEDSYSISRCIQILNSMAELSQVEKSVAPDVFLQPGNREIFVSLGIDVRALWLRRKIQHLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HL80</accession>
    <name>I1HL80_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100838785</name>
        <name evidence="6" type="ORF">BRADI_2g33682v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the heat shock protein 70 (TC 1.A.33) family. HSP110/SSE subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315948.1">
        <property type="nucleotide sequence ID" value="XM_024460180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HL80"/>
    <dbReference type="STRING" id="15368.I1HL80"/>
    <dbReference type="EnsemblPlants" id="KQK07194">
        <property type="protein sequence ID" value="KQK07194"/>
        <property type="gene ID" value="BRADI_2g33682v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK07195">
        <property type="protein sequence ID" value="KQK07195"/>
        <property type="gene ID" value="BRADI_2g33682v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838785"/>
    <dbReference type="Gramene" id="KQK07194">
        <property type="protein sequence ID" value="KQK07194"/>
        <property type="gene ID" value="BRADI_2g33682v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07195">
        <property type="protein sequence ID" value="KQK07195"/>
        <property type="gene ID" value="BRADI_2g33682v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0103">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_5_1_1"/>
    <dbReference type="OMA" id="YFYRHKL"/>
    <dbReference type="OrthoDB" id="434160at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd24095">
        <property type="entry name" value="ASKHA_NBD_HSP70_AtHsp70-14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000043">
        <property type="entry name" value="Heat shock 70 kDa protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000171">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000018">
        <property type="entry name" value="Heat shock 70 kDa protein 4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF25">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="499"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="778"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="509"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="550"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="784"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="798"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK07194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="843" mass="93344" checksum="82A543A418FFE7B1" modified="2012-06-13" version="1">MSVVGFDLGNESCIVGVARQRGIDVVLNEESKRETPAIVCFGDKQRFIGTAGAANSTMNPKNSISQIKRLLGRKFTDPELQHDLQSFPFHVSEGPDGFPLVHARYLGEERSFTPTQLLAMVLSNLKGIAEGNLNSAVIDCCIGIPVYFTNLQRRAVLDAATIAGLRPLRLFHETTATALAYGIYKTDLPENDQLNVAFVDVGHASMQVSIVGYKKGQLKMLSHAYDRSLGGRDFDEALFKHFAAKFKEEYKIDVYQNARACIRLRVACEKLKKMLSANPEAPMNIECLMDEKDVRGFIKRDEFEQISGPVLERVKGPLEKALAEAGLTTESVHFVEVVGSGSRVPAIMRIITEFFGKEPRRTMNASECVARGCALQCAILSPTFKVREFQVNEGFPFSIALSWKSDAQSNESQQTVVFPKGNPMPSIKALTFYRSNTFAVDVLNVDTDDLQITQKISTYTIGPFQPSNGEKAKVKVKVRLNIHGIVSLESATMLEEDEVEVPVSSASEVPKDATKMDTDDAQRDPASGNDVNMEDSKGATDTAEGAVENGAHDSEEKSVPMDTDTKVQPSKKRVKKTNVPIAELVYGTLGADELEKAVEKEYEMALQDRVMEETKEKKNSVEAYVYEMRNKLSEKYNDFVMSEDMEVLMAKLQEVEDWLYEDGEDETKGVYVAKLEELKKVGGPIEMRYKEWSERGQALEQLVYCIRSFREAALSSDQKFDHIDISEKQKVVNECSGAETWLLEKKQQQDALPKHVNPVLLVSDIKKKAEALDRFCKPIMTKPKPAPKPQTPPPVETPAPEAQTPEQQSSGAGEADEPANDGGAQDQPAAEQMDTDSAEPSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1HLF7</accession>
    <name>I1HLF7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100827674</name>
        <name evidence="3" type="ORF">BRADI_2g34540v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568853.1">
        <property type="nucleotide sequence ID" value="XM_003568805.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HLF7"/>
    <dbReference type="STRING" id="15368.I1HLF7"/>
    <dbReference type="EnsemblPlants" id="KQK07319">
        <property type="protein sequence ID" value="KQK07319"/>
        <property type="gene ID" value="BRADI_2g34540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827674"/>
    <dbReference type="Gramene" id="KQK07319">
        <property type="protein sequence ID" value="KQK07319"/>
        <property type="gene ID" value="BRADI_2g34540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827674"/>
    <dbReference type="eggNOG" id="ENOG502S11E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094736_0_0_1"/>
    <dbReference type="OMA" id="GCRTAVW"/>
    <dbReference type="OrthoDB" id="1856818at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33052">
        <property type="entry name" value="DUF4228 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094712" evidence="2">
        <location>
            <begin position="18"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK07319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="162" mass="16994" checksum="CE8C2D2538F648CB" modified="2012-06-13" version="1">MGNSLRCCLACVLPCGALDLIRIVHLSGRVEEYGRPVAAGEILAANPNHVLSKPCSQGGGGGVRRILIVSPESELERGEIYFLIPASSVPERKKKSGGGSSPGPAAAAGCQKKAPAEKSSSHVKSKAACEVAEKRSMHRRRVSSGSRAAVWRPHLECIVEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPC7</accession>
    <name>I1HPC7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100824691</name>
        <name evidence="6" type="ORF">BRADI_2g43380v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569350.1">
        <property type="nucleotide sequence ID" value="XM_003569302.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPC7"/>
    <dbReference type="FunCoup" id="I1HPC7">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK08700">
        <property type="protein sequence ID" value="KQK08700"/>
        <property type="gene ID" value="BRADI_2g43380v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824691"/>
    <dbReference type="Gramene" id="KQK08700">
        <property type="protein sequence ID" value="KQK08700"/>
        <property type="gene ID" value="BRADI_2g43380v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824691"/>
    <dbReference type="eggNOG" id="ENOG502QSMM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015101_2_1_1"/>
    <dbReference type="OMA" id="PSILCGH"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF673">
        <property type="entry name" value="OS01G0612900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094685" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="30"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK08700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="43687" checksum="2C6315E2CE3BF292" modified="2012-06-13" version="1">MAFAAGAARLAAAAALVAAVVVMPGPAEGGVCFQRIFSFGDSLTDTGNFVLSVPDDFPDPARSLPYGQTFFGRPSGRYSDGRNLLDFFAEAFGLPYVPPYLGGGDFLNGANFAVGGATALNGSFFRDLGVEPTWTPHSLDEQIQWFKNLLSSIASSESEHRDVMSKSLFLVGEVGGNDYNHLIVRGKSLDELHKLVPNVVGVISSAITELINLGARKLVVPGNFPIGCVPLYLAIFPSQKEGYYNEKTGCIEWLNEFTEYHNRLIQEELDKLRNLHPDVSLIYADYYGATLDIYRAPLQFGFTVPLNSCCGSDAPHNCSPSVMCGNPGSFVCPDPSKYISWDGLHFTEATYKVIIQGVLGSYAFPPLSETCRGGEYKVSQLHQCTENNPTNTVTYDAMSSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQB2</accession>
    <name>I1HQB2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tubby-like F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100833695</name>
        <name evidence="5" type="ORF">BRADI_2g46350v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569549.1">
        <property type="nucleotide sequence ID" value="XM_003569501.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQB2"/>
    <dbReference type="STRING" id="15368.I1HQB2"/>
    <dbReference type="EnsemblPlants" id="KQK09158">
        <property type="protein sequence ID" value="KQK09158"/>
        <property type="gene ID" value="BRADI_2g46350v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833695"/>
    <dbReference type="Gramene" id="KQK09158">
        <property type="protein sequence ID" value="KQK09158"/>
        <property type="gene ID" value="BRADI_2g46350v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833695"/>
    <dbReference type="eggNOG" id="KOG2502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028236_3_0_1"/>
    <dbReference type="OMA" id="PPLQCFI"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HQB2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018066">
        <property type="entry name" value="Tubby_C_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF147">
        <property type="entry name" value="TUBBY-LIKE F-BOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01200">
        <property type="entry name" value="TUB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01201">
        <property type="entry name" value="TUB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="4">
        <location>
            <begin position="100"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK09158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="360" mass="40637" checksum="DF376508FB498E80" modified="2012-06-13" version="1">MPREKPAAERGEVPEVVEVEEKAEEGGQAEAEEQEERWARLLPELLSEVVRRVEASGGERWPARKDVVSCACVCRRWRDAAVAVVRPPAVSGKITFPSSLKQPGTREFPMQCFIKRNKKNSTFYLYLGLTTATVDKGKFLMAARRFRRGPHTEYIISLDADDLSQGSNAYMGKLRSDFWGTNFKVYDSKPPYDGAKASSSRSSRRFGSRRISPQVSAGNYEVGQLSYKYNLLKSRGPRRMYCALECPSTQETWENSLKSKFQKPMGTTVLRNKAPRWHEHLQCWCLNFHGRVTVASVKNFQLVAAADPNDPTGSRDEDTVLLQFGKVDDDIFTMDYRQPLSAFQAFAISLSSFGTKLACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HRI1</accession>
    <name>I1HRI1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100832332</name>
        <name evidence="3" type="ORF">BRADI_2g49700v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569800.1">
        <property type="nucleotide sequence ID" value="XM_003569752.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HRI1"/>
    <dbReference type="EnsemblPlants" id="KQK09711">
        <property type="protein sequence ID" value="KQK09711"/>
        <property type="gene ID" value="BRADI_2g49700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832332"/>
    <dbReference type="Gramene" id="KQK09711">
        <property type="protein sequence ID" value="KQK09711"/>
        <property type="gene ID" value="BRADI_2g49700v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832332"/>
    <dbReference type="eggNOG" id="KOG0192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_7_35_1"/>
    <dbReference type="OMA" id="KLKHTHH"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF161">
        <property type="entry name" value="OS01G0747400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="283"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK09711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="594" mass="66317" checksum="D923FEDEE7212CFD" modified="2012-06-13" version="1">MDDEDYSWVRRTRFSHSVVRSNSGREQFGAFVEQFNRGAALKQKGSDSGFKLHGLNFEPKTKTSSATNSANSSVLRSRSSSAQPKDSSSNGKPAQHDKASDPGLPSQQALTKQEDSSGNGTKGSNLFVDVCCQPEVQESKAEIPVPLEFSFHPDEQSLRLQRVCSSPSPFPSEKMPAFDAPPARSSSLKIIGDGRQPMLRARSPIPSRPVPQVFKEAKSESKRFSTPPPRRKSLSPPRASPVVEPARAPGKVKHRKECRDNGRAAKAAALKVLEKWSVDRSQLLIGHRFASGAHSRLFHGIYQEQPVAVKFIRLPDDEEEAELSAQLEKQFSTEITMLSHLHHRNVIKLVGACSSPPVFCVLTEFLSGGSLRAFLHKQEHKSLPLEKIISVGLDIAHGMAYIHSQGVVHRDVKPENIIFDGECCAKIVDFGIACEEAYCDPLANDPGTFRWMAPEMMKHKPYGRKVDVYSFGLILWEMLTGSVPYDDLTPFQAAFAVFDKNVRPTIPVSCPAALRLLIEQCWALQPDKRPEFWQIVQLLEKFKMFLDRDGTLDNMPSSNCQETHDHKKWLAHWVHKLKHSHHHDLSGPPPPKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HRR9</accession>
    <name>I1HRR9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100821407</name>
        <name evidence="3" type="ORF">BRADI_2g50360v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232350.2">
        <property type="nucleotide sequence ID" value="XM_010234048.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HRR9"/>
    <dbReference type="STRING" id="15368.I1HRR9"/>
    <dbReference type="EnsemblPlants" id="KQK09822">
        <property type="protein sequence ID" value="KQK09822"/>
        <property type="gene ID" value="BRADI_2g50360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821407"/>
    <dbReference type="Gramene" id="KQK09822">
        <property type="protein sequence ID" value="KQK09822"/>
        <property type="gene ID" value="BRADI_2g50360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821407"/>
    <dbReference type="eggNOG" id="KOG1603">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100095_0_1_1"/>
    <dbReference type="OMA" id="MTLVEMC"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF351">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK09822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="141" mass="15167" checksum="A2D1337BC4D0CBE2" modified="2012-06-13" version="1">MTIVEMCVHMCCAGCEKKIRKAVEKLEGVDGVEIDMEMQKVTVNGDVEQKKVLKAVRRTGKRAVLWPSTPYNIPGAGAAHLLLAQPAGGAHTYAAGPTSSYNYYKHGYDDSRLYGANSSLVGGTRATDYFSDENTGGCSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I1HS70</accession>
    <name>I1HS70_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BRADI_2g51560v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HS70"/>
    <dbReference type="FunCoup" id="I1HS70">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK10021">
        <property type="protein sequence ID" value="KQK10021"/>
        <property type="gene ID" value="BRADI_2g51560v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10021">
        <property type="protein sequence ID" value="KQK10021"/>
        <property type="gene ID" value="BRADI_2g51560v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWDY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_35_7_1"/>
    <dbReference type="InParanoid" id="I1HS70"/>
    <dbReference type="OMA" id="DSTCTYV"/>
    <dbReference type="OrthoDB" id="667626at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000007">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000343">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000806">
        <property type="entry name" value="Putative DUF26-domain protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000032">
        <property type="entry name" value="Putative DUF26-domain protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF498">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094774" description="Protein kinase domain-containing protein" evidence="18">
        <location>
            <begin position="34"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="20">
        <location>
            <begin position="53"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="20">
        <location>
            <begin position="163"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="377"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="34"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="288"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQK10021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="697" mass="75433" checksum="6385B3870CC5C785" modified="2012-06-13" version="1">MQQSLNAKHFSPAPPLVLLLLLLAAASIPASHAGVNCGSSSQPSPAASASSPSPSPAPAPTNSNSSSSSAFRTNLLTLLDALPRAAAATGFASLSTPGTGPDRAFVRGLCRGDSTPSECLSNLQSAARDIAGRCPSSSRSAGGWYEKCYITYADTNDTAAYEQLIRALVVDARRVSDPDGYEETYYALMDRLVARAASTGGNGSARTFFATGEAEYARGEPDGTMYGLVQCMRDRSAVECQRCLRAIVPWLPLCCRGQSGGVAQNFNCHLRIQIYTYYDVALDAPPPASPSLPELPSPSPAGDRQGKRRSGHVILTATLLPVGTLLFLAAVLVCVYLQRRRVKANTEPRDDTRDEDTTYVHPEQFTLPLLRAATGNFAAENKLGEGGFGQVFKGKLPDGQAIAVKRLSKSSSQGFHELKNELVLAAKLRHRNLVHLLGVCLEEQEKLVVYEYLPNRSLDTVLFDNGRRRRRHGLDWGKRHAIIRGIARGLQYLHVDSRLRVIHRDLKPSNVLLDESMNPKISDFGLARAFRGDQSRDVTRRAAGTLGYMAPEYAYCGHVSTKSDIFSFGVILLEIVTGRRNNSPSQDTNTKNLLSDVWEKWRAGSVAEIADASLGDLYPRAEMLRCVHVGLLCVQKEPALRPDASEVVLILHSQSTSLQVPSRPAFYSGTTVSGVASRVGTANVSVNGVTMSEFEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUC7</accession>
    <name>I1HUC7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Two-component response regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100824670</name>
        <name evidence="15" type="ORF">BRADI_2g58020v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional activator that binds specific DNA sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARR family. Type-B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564868.1">
        <property type="nucleotide sequence ID" value="XM_003564820.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUC7"/>
    <dbReference type="STRING" id="15368.I1HUC7"/>
    <dbReference type="EnsemblPlants" id="KQK11088">
        <property type="protein sequence ID" value="KQK11088"/>
        <property type="gene ID" value="BRADI_2g58020v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824670"/>
    <dbReference type="Gramene" id="KQK11088">
        <property type="protein sequence ID" value="KQK11088"/>
        <property type="gene ID" value="BRADI_2g58020v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824670"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024359_3_0_1"/>
    <dbReference type="OMA" id="ETMIMEC"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HUC7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17584">
        <property type="entry name" value="REC_typeB_ARR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000132">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017053">
        <property type="entry name" value="Response_reg_B-typ_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF137">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036392">
        <property type="entry name" value="RR_ARR_type-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="14">
        <location>
            <begin position="13"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="267"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="12">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR036392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK11088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="577" mass="64367" checksum="55FC21CD8351471D" modified="2012-06-13" version="1">MAADATVFPYGLRVLVVDDDPTWLKILEKMLRKCSYEVTTCGLARVALEILRERKSKFDIVISDVNMPDMDGFKLLEHIGLEMDLPVIMMSIDGETSRVMKGVQHGACDYLLKPVRMKELRNIWQHVYRKRMHEVKEIEGHDSCDDLQILRYGFEGFDEKGIFITADSDTTRKRKDLDKDHADQDSSDGATAKKARVVWSVDLHQKFVNAVNQIGFDKVGPKKILDLMNVPGITRENVASHLQKYRLYLGRLQKQNEERILGAARQDFSNKGTSENLNLRSSFQDQPSNVSSGYPHASQKIQGQSSMLDSQLEDTKCTVPLPASDKSRNSVNDVADSQNVADASPLSGVLSFKGLPVNQDNNPSETMIMECQTWNGGVPSKQFMQYPKHNHARCDLLGDYACLPKPDLEHPAAPGNFFTPPPLISMSCSMELDARNFSDVKPALLDCIKSLSPALTCTVDSVSVQLSDSVVTSTSSDRKFSTVEGLPYFDQTNSQGPLFRSQEPSTTCGLDLASLPEDLPSYPLQGVPFENIGLSSIDLFHYNDTMLLAGLQSNWYDDQEFISETTDYPLMDGCLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXJ9</accession>
    <name>I1HXJ9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100822144</name>
        <name evidence="15" type="ORF">BRADI_3g04860v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233857.1">
        <property type="nucleotide sequence ID" value="XM_010235555.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXJ9"/>
    <dbReference type="STRING" id="15368.I1HXJ9"/>
    <dbReference type="EnsemblPlants" id="KQJ93480">
        <property type="protein sequence ID" value="KQJ93480"/>
        <property type="gene ID" value="BRADI_3g04860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822144"/>
    <dbReference type="Gramene" id="KQJ93480">
        <property type="protein sequence ID" value="KQJ93480"/>
        <property type="gene ID" value="BRADI_3g04860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822144"/>
    <dbReference type="eggNOG" id="KOG2288">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040730_3_0_1"/>
    <dbReference type="OMA" id="HNDFMRI"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HXJ9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000011">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025298">
        <property type="entry name" value="DUF4094"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF273">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13334">
        <property type="entry name" value="DUF4094"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="DUF4094" evidence="14">
        <location>
            <begin position="21"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQJ93480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="408" mass="45563" checksum="4EA719F4B1636D86" modified="2012-06-13" version="1">MSWRRGSGGGGGGGGGDGGVSRRWAVLLCLGSFCIGLLFTNRMWTLPEATEIARPNQKDDEGGALVAGDCGSKKIQELHNYKDELPVQDTHHDVQTLDKTIASLETELSAARTLQESLLNGSPVAEEFKVSESIVRRKYRMVIGINTAFSSRKRRDSIRYTWMPRGEQRKKLEEEKGIIIRFVIGHSAISGGIIDRAIEAEDRKHGDFMKIDHVEGYLALSGKTKTYFATAVSLWDADFYVKVDDDVHVNIATLGQILSKHAWKPRVYIGCMKSGPVLSEKGVRYYEPEHWKFGEPGNKYFRHATGQLYAISKDLATYISINKHVLHKYINEDVSLGSWFLGLDAEHIDEKRLCCGTPPDCEWKAQAGNICAASFDWRCSGICNSEGRIWEVHNKCAEGESALWNSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZ63</accession>
    <name>I1HZ63_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100832249</name>
        <name evidence="6" type="ORF">BRADI_3g09220v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234087.1">
        <property type="nucleotide sequence ID" value="XM_010235785.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZ63"/>
    <dbReference type="STRING" id="15368.I1HZ63"/>
    <dbReference type="EnsemblPlants" id="KQJ94206">
        <property type="protein sequence ID" value="KQJ94206"/>
        <property type="gene ID" value="BRADI_3g09220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832249"/>
    <dbReference type="Gramene" id="KQJ94206">
        <property type="protein sequence ID" value="KQJ94206"/>
        <property type="gene ID" value="BRADI_3g09220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832249"/>
    <dbReference type="eggNOG" id="ENOG502RRI4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058719_3_2_1"/>
    <dbReference type="OMA" id="HCESAGM"/>
    <dbReference type="OrthoDB" id="687020at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000003">
        <property type="entry name" value="Blue copper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF545">
        <property type="entry name" value="OS09G0572700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094906" description="Phytocyanin domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="5">
        <location>
            <begin position="23"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="125"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ94206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="175" mass="17644" checksum="78B98E2CD5066D34" modified="2012-06-13" version="1">MAAMRIALLALAAMAVLGTASAATYNVGEPGGAWDLRTDYGNWASSKKFHPGDTIVFKYSPAQHDVLEVSKADYDSCNTNSPISTLTTGNDVVSLTSTGTRYFICGFPGHCTTSGTGLMKVKIDVTPGSSSNSPAPAAGPGASNSPPPPPSSAATSVGPTAGFGFAVVLLACLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZV0</accession>
    <name>I1HZV0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100824201</name>
        <name evidence="2" type="ORF">BRADI_3g11210v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573104.1">
        <property type="nucleotide sequence ID" value="XM_003573056.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZV0"/>
    <dbReference type="FunCoup" id="I1HZV0">
        <property type="interactions" value="2720"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HZV0"/>
    <dbReference type="EnsemblPlants" id="KQJ94563">
        <property type="protein sequence ID" value="KQJ94563"/>
        <property type="gene ID" value="BRADI_3g11210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824201"/>
    <dbReference type="Gramene" id="KQJ94563">
        <property type="protein sequence ID" value="KQJ94563"/>
        <property type="gene ID" value="BRADI_3g11210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824201"/>
    <dbReference type="eggNOG" id="KOG1299">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013933_3_1_1"/>
    <dbReference type="OMA" id="VHQLNNA"/>
    <dbReference type="OrthoDB" id="10266265at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HZV0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009846">
        <property type="term" value="P:pollen germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000012">
        <property type="term" value="P:regulation of auxin polar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000067">
        <property type="term" value="P:regulation of root morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031338">
        <property type="term" value="P:regulation of vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ94563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="567" mass="64428" checksum="BD7DE5E5EAE5A441" modified="2012-06-13" version="1">MVLIPLVRDYIDRMLHDISGMKVLVLDPETVGMVSVVYSQSDLLKKEVFLVETVDDASSSKASMAHLKAVYFLRPSSENVQKLRRHLAAPRFAECHLFFSNILKIPQIQVLADSDEQEVVQQVQEFYADFCAIDPFHFTLNIHNNHIYMLRTVVDPPGMQSFCDRAVDGIASVFLALKRRPVIRCQRTSDVAKRIAQETARLMYEQESGLFDFRRTENSSLLLVIDRRDDPVTPLLNQWTYQAMVHELIGIENNKVDLKEFANVPKDQQEVVLSAVQDDFFRANMFENFGDLGMNVKRMVDDFQHLSKSSQNFQSIGDMAKFVANYPEYRKTHGNVTKHVALVSEMSRMVEERKLMQVSQTEQELACTSGQAAAFEAVTSLLNNQNVSDIDRLRLVMLYALRYEKESPVQLMQLFNKLASRSAKYKSGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLFQTMEGIVKGRLRDADYPLVGNHFQQGRPQDVVIFIVGGTTYEEARSVALYNAANPGVRFFLGGSVVLNSKRFLEDLGEAQRILKSSTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1I274</accession>
    <name>I1I274_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100822348</name>
        <name evidence="11" type="ORF">BRADI_3g18700v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAUS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573589.1">
        <property type="nucleotide sequence ID" value="XM_003573541.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I274"/>
    <dbReference type="STRING" id="15368.I1I274"/>
    <dbReference type="EnsemblPlants" id="KQJ95722">
        <property type="protein sequence ID" value="KQJ95722"/>
        <property type="gene ID" value="BRADI_3g18700v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66937">
        <property type="protein sequence ID" value="PNT66937"/>
        <property type="gene ID" value="BRADI_3g18700v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66938">
        <property type="protein sequence ID" value="PNT66938"/>
        <property type="gene ID" value="BRADI_3g18700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822348"/>
    <dbReference type="Gramene" id="KQJ95722">
        <property type="protein sequence ID" value="KQJ95722"/>
        <property type="gene ID" value="BRADI_3g18700v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66937">
        <property type="protein sequence ID" value="PNT66937"/>
        <property type="gene ID" value="BRADI_3g18700v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66938">
        <property type="protein sequence ID" value="PNT66938"/>
        <property type="gene ID" value="BRADI_3g18700v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822348"/>
    <dbReference type="eggNOG" id="ENOG502QSQA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077821_0_0_1"/>
    <dbReference type="OMA" id="CEAQMES"/>
    <dbReference type="OrthoDB" id="5372507at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026243">
        <property type="entry name" value="HAUS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570:SF1">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25762">
        <property type="entry name" value="HAUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT66938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="310" mass="33534" checksum="7D70A0BC5E194F4F" modified="2012-06-13" version="1">MDHAAEHLDTAAPASAPASVAEVNAWLASLATEGGGVGGRGGGGSAAASELSLGPDPTPRGVSYLRALAAASQARSRAAGIAASGLRAQAAEYRAEAARLREALERAGLARDALPSPAASAARAVAAVANLLAIRDTEMSSFVVASADLWLRRAEVEEKRDRVYKESKALLDYTRKAITKLTELKKMLEKFKNDVEKQQVEQMTDWQTKLVMMDSKERQYILQVSNYKAMLNRVGYTPEINHGVLMEMAEHKKDLERKTKPIADTLRSYQDLPPDKALAALAIEDKKRQYAAAEKYLEDVLQSALTTPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1I496</accession>
    <name>I1I496_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100838813</name>
        <name evidence="9" type="ORF">BRADI_3g27450v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573968.1">
        <property type="nucleotide sequence ID" value="XM_003573920.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I496"/>
    <dbReference type="FunCoup" id="I1I496">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ96855">
        <property type="protein sequence ID" value="KQJ96855"/>
        <property type="gene ID" value="BRADI_3g27450v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838813"/>
    <dbReference type="Gramene" id="KQJ96855">
        <property type="protein sequence ID" value="KQJ96855"/>
        <property type="gene ID" value="BRADI_3g27450v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838813"/>
    <dbReference type="eggNOG" id="ENOG502QVVV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027462_0_3_1"/>
    <dbReference type="OMA" id="ANWQCLS"/>
    <dbReference type="OrthoDB" id="628427at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095093" description="Expansin" evidence="6">
        <location>
            <begin position="24"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="7">
        <location>
            <begin position="43"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="8">
        <location>
            <begin position="167"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ96855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="252" mass="27010" checksum="40EBC42CEF2A6C23" modified="2012-06-13" version="1">MDMAKSLVLCAIFLSACLSLAAADWLQATATFYGEADASGTMGGACGYSNLYDQGYGVNNAALSTVLFNNGASCGQCFTITCDSKKSGWCKTGNSITVSATNLCPPNWALPNDNGGWCNPPRQHFDMSQPAWETIAIYRAGIVPVLYQRVKCSRQGGVRFTISGFNYFELVLITNVGGSGSVASVSIKGSNTGWIQMSRNWGANWQSLAGLAGQALSFAVTTTGGQYLVFDNIAPVWWQFGQTFGSYKQFDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICR8</accession>
    <name>I1ICR8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100831352</name>
        <name evidence="8" type="ORF">BRADI_3g52096v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235838.1">
        <property type="nucleotide sequence ID" value="XM_010237536.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICR8"/>
    <dbReference type="FunCoup" id="I1ICR8">
        <property type="interactions" value="945"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00831">
        <property type="protein sequence ID" value="KQK00831"/>
        <property type="gene ID" value="BRADI_3g52096v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831352"/>
    <dbReference type="Gramene" id="KQK00831">
        <property type="protein sequence ID" value="KQK00831"/>
        <property type="gene ID" value="BRADI_3g52096v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831352"/>
    <dbReference type="eggNOG" id="KOG1237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009313_4_1_1"/>
    <dbReference type="OMA" id="RETSGKW"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="525"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="569"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="595"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK00831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="619" mass="67031" checksum="3CEBFCFB244650AA" modified="2012-06-13" version="1">MSANDGDMKMRVIVMDSEAERSAAKEKLCEYTLDGSVDIKGRPAVKGKSGGWLAGGLILVNQGLATMAFFGVNVNLVLFLTRVVQQSNGDAANNVSKWTGTVYMFSLIGAFLSDSYWGRYKTCAIFQAIFVLGLALLSLSSRLYLIRPFGCGTQGTPCAPHSGAEMGIFYIALYMIAFGNGGYQPNIATFGADQFDEEDPAEAHSKVSFFSYFYLALNLGSLFSNTFLSYIQDKGNWVLGFWASTAAAATALLLFLSGTPQYRHAQPCGNPIGSICQVASAACRNWKSGVSPDHEVLYEGDDGSKADSGGRKLLHTEGFRFLDRAALTKASDSNTKLGGACPGPRDQWKLCTVTQVEQVKSILRLLPIWLCTILYSVVFTQMASLFVVQGAAMRRTTMFPGFYVPASSMSAFDILTVATTIFLYRRAICPFLARLTGRPTGPTELQRMGLGLVLGAMAMATAGTVEHFRKAGATGTAANSSDLHIIWQVPQYALIGVSEVMMYVGQLEFFNGEMPDGFKSFGSALCMMSMSLGNYFSDVILTAVTRATAVDGRPGWVPDDLNEGHLNKFYFLLAILAVADFAVYLVFASRYRRSGNKVDGRSDDDEEEGSVHGQVAYAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1ID10</accession>
    <name>I1ID10_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100822547</name>
        <name evidence="2" type="ORF">BRADI_3g52780v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570192.1">
        <property type="nucleotide sequence ID" value="XM_003570144.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ID10"/>
    <dbReference type="STRING" id="15368.I1ID10"/>
    <dbReference type="EnsemblPlants" id="KQK00942">
        <property type="protein sequence ID" value="KQK00942"/>
        <property type="gene ID" value="BRADI_3g52780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822547"/>
    <dbReference type="Gramene" id="KQK00942">
        <property type="protein sequence ID" value="KQK00942"/>
        <property type="gene ID" value="BRADI_3g52780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822547"/>
    <dbReference type="eggNOG" id="ENOG502QWN6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="CLHNCKI"/>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ID10">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47583">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47583:SF1">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="44"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK00942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="180" mass="19893" checksum="8847FCDBDE0AA498" modified="2012-06-13" version="1">METVEEDVEEYSWREVVLPRLIPVVQDAAPELERETGERRRGRDLLVAVDFGPNSKHAFDWALVHLARMADTVHLVHAVSSVHNDLVYDKSQELMEDLAIEAFKVSLVRTKARIVEGNAGKAICLEAERLKPAAVILGTRGRGLIQSVLQGSVSEYCFHNCKAAPVIIVPGKEAGEQSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEW6</accession>
    <name>I1IEW6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA repair and recombination protein RAD54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100831864</name>
        <name evidence="17" type="ORF">BRADI_3g58092v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <sequence length="965" mass="107056" checksum="2431D1503E56EE0B" modified="2012-06-13" version="1">MRPSRSRCDRRGSDADEEEVVALSSDPDESESEPEGGAEVDDDEEYVGESSDAGGVDDEAEEGGGGSDNGGGGRLVRGARRGAVAPDEERKSQNVDALVRGNLVVRRQPLIPRILSVSDAAAIARKPFKPPCENGYSENNEHLARRLSARKRFVPWGSMQPFAVISNLPQSSTIASDDSLEKEESLPPGIEPLILWQPEGCDKENNNFAAIEVDNLLVRYLRPHQREGVQFMFDCVSGLLSDDGIAGCILADDMGLGKTLQSIALLYTLLAQGFDGKPMVKRAVIVTPTSLVSNWESEISKWLKGKVQLLALCESTRADVLSGIGSFLKPLSRLQVLIISYETFRMHSSKFERPASCDLLICDEAHRLKNDQTLTNKALAALPCTRRILLSGTPMQNDLEEFFSMVNFTNPGVLGDAAYFRRYYEAPIICGREPTATAEEKKLGSERSGELSAKVNQFILRRTNALLSNHLPPKIVEVVCCKLTPLQTTLYNHFIHSKNVKRLISEEAKRSKILAYITALKKLCNHPKLIYDTIKSNKSGGSDFDDCLRFFPPELFQGRSGSWTGGGGMWVELSGKMHVLARLLGHLRLKTDDRIVLVSNYTQTLDLFAQLCRERRYPYVRLDGATSINKRQKLVNQFNDLSRDEFVFLLSSKAGGCGLNLVGGNRLVLFDPDWNPANDKQAAARVWRDGQKKRVYIYRFLSTGTIEEKVYQRQMSKEGLQKVIQQEQGNTKMQDQGCSLSTEDLRDLFTFHEQVRSEIHENLKCNRCNKDSYMLLDGNSLESAAIEHNKISTSGEKYYTDIGGFGEISGCVQKMNSSNQQIDQPSEEDLGSWGHHSDPSTVPDTILQSSAGDEVSFVFTNQIDGKLVPVESMVRLTTPNGMTASGEKQVAEINSPNKPGWQSSLGKNLKMTGFNLKNSSLKCPTKSRRTSPICLQGLNKTNSSDYKPQTKKLHVASDMSDDDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1IGD0</accession>
    <name>I1IGD0_BRADI</name>
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        <recommendedName>
            <fullName evidence="6">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">LOC100836094</name>
        <name evidence="3" type="ORF">BRADI_4g01480v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
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        <property type="protein sequence ID" value="KQJ85756.1"/>
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        <property type="protein sequence ID" value="PNT62306.1"/>
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        <property type="protein sequence ID" value="KQJ85756"/>
        <property type="gene ID" value="BRADI_4g01480v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT62306"/>
        <property type="gene ID" value="BRADI_4g01480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836094"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35997:SF6">
        <property type="entry name" value="COTTON FIBER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35997">
        <property type="entry name" value="COTTON FIBER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF761"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="2">
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            <begin position="58"/>
            <end position="80"/>
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            <begin position="154"/>
            <end position="239"/>
        </location>
    </feature>
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            <begin position="171"/>
            <end position="182"/>
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            <end position="208"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT62306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="30736" checksum="4359BA281A50EC24" modified="2012-06-13" version="1">MLPLVNMQAPDHNHHPDKTTRQMEMMRKVSISIIVMALPVLYASVLRVPPATLLRDTTFWFLFSNSIIVFIAADSGTLFFTPSSSSSSDHAVDDDRPFVVSVSGDFAPPTMVLDEYSGANDGHGDAAAAIDEHDVNMPEREETTAMAMWMSAPPSSALTAGVDSGATVRPAMRRRRRSRSHSSRALVVAPPPEQEEKSVVVQEETTTKQLRRTATEPRPPAPEKKEKEDEESEYYARLSDEELNRRVEDFIARFNREIRLQVEREDQQTLPQAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1IGF9</accession>
    <name>I1IGF9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PORR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100842188</name>
        <name evidence="3" type="ORF">BRADI_4g01740v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579141.2">
        <property type="nucleotide sequence ID" value="XM_003579093.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IGF9"/>
    <dbReference type="STRING" id="15368.I1IGF9"/>
    <dbReference type="EnsemblPlants" id="KQJ85793">
        <property type="protein sequence ID" value="KQJ85793"/>
        <property type="gene ID" value="BRADI_4g01740v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT62323">
        <property type="protein sequence ID" value="PNT62323"/>
        <property type="gene ID" value="BRADI_4g01740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842188"/>
    <dbReference type="Gramene" id="KQJ85793">
        <property type="protein sequence ID" value="KQJ85793"/>
        <property type="gene ID" value="BRADI_4g01740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62323">
        <property type="protein sequence ID" value="PNT62323"/>
        <property type="gene ID" value="BRADI_4g01740v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842188"/>
    <dbReference type="eggNOG" id="ENOG502QRK6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024287_6_1_1"/>
    <dbReference type="OMA" id="ILYKCRS"/>
    <dbReference type="OrthoDB" id="689415at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021099">
        <property type="entry name" value="PORR_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045040">
        <property type="entry name" value="PORR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31476">
        <property type="entry name" value="PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31476:SF2">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11955">
        <property type="entry name" value="PORR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PORR" evidence="2">
        <location>
            <begin position="63"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ85793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="490" mass="54543" checksum="C6E29D1EE3EE7549" modified="2012-06-13" version="1">MAASSLSFLTVAHHPIFPLNPGRLRRPHPATPTPKPLSAAIRCSATAPPAAARARPIPPPKLVRCPALDRHASRASRLRFARKLLTLLLSKPRRFLPLRVLRRCRRFLGVPRDRPLVPFVLRYPAIFRLFHAPVSFPLSPSLSTLAVALTPAAHALADDLAALRGSELAPGLAAKFHRLLLMTPRHSLLVSKLAHLAPDLGLAMDFRATLCPNHPDLFTFAHTSHGHALRLVDDPTPPPSPSPLPPPVRSADRLIDRPRRFPHLPLRRGLNLRRAHRDYLLRFHSLPEVSPFEPLEEGASAEMAERRACAVVREVLAMTVEKRTLVDHLTHFRRDFGLPNRLRAMLVRHPEMFYVSIKGVRHSVFLVEAFDDDGRLLVEDEMLVGRDRLEELVREGKRIRRARKKGDLAVDGDSDEDEVEDDNVVDDSLGADGEFGDLFEAGVAGDDWNEVGGIEGDNEGDAESDAMEEFWVKKAVAEGLVDSGSEHDVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IHE1</accession>
    <name>I1IHE1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100845646</name>
        <name evidence="5" type="ORF">BRADI_4g04327v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237144.1">
        <property type="nucleotide sequence ID" value="XM_010238842.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IHE1"/>
    <dbReference type="EnsemblPlants" id="KQJ86261">
        <property type="protein sequence ID" value="KQJ86261"/>
        <property type="gene ID" value="BRADI_4g04327v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845646"/>
    <dbReference type="Gramene" id="KQJ86261">
        <property type="protein sequence ID" value="KQJ86261"/>
        <property type="gene ID" value="BRADI_4g04327v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845646"/>
    <dbReference type="eggNOG" id="ENOG502R6G1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015101_0_2_1"/>
    <dbReference type="OMA" id="PMENGSC"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF23">
        <property type="entry name" value="GDSL-LIKE LIPASE_ACYLHYDROLASE FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095317" description="GDSL esterase/lipase" evidence="4">
        <location>
            <begin position="32"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ86261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="40420" checksum="7E3CE86BFF176953" modified="2012-06-13" version="1">MTRRCAAAALLLAVAASAALLVCSSAPGVPAVYVFGDSLVDVGNNAFLSPPAPRAAFPCGLDLPPGGRSTGGRFTNGYNLADIVAQRMGFNMSPPAYLSLTPQTSLNLLRGQVGANYASGGSGILNITGNGTITLQEQVQLFVNTKASMIDSGKLGNDMANRLLSRSLFLVSTGGNDFAAFTEGRVTIAEAPAYIASMVSTYIKHIKALYKLGARRLGILDVLPVGCVPSTRTWSSDGVCDAPANSLARGFNTLLRAEMANAAAAAMPDLIYSIASIYNIFYDMINNPQLDGLEEVASACCGGGRLNAEDDCSARSNLCADRDRYVFWDKVHGTQAAYKRAVAAMFDGVGAGRYTEPISFEQLVGNQVLLMQGPMVDLELQSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1IHE3</accession>
    <name>I1IHE3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106866656</name>
        <name evidence="17" type="ORF">BRADI_4g04347v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757736.1">
        <property type="nucleotide sequence ID" value="XM_014902250.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IHE3"/>
    <dbReference type="EnsemblPlants" id="KQJ86265">
        <property type="protein sequence ID" value="KQJ86265"/>
        <property type="gene ID" value="BRADI_4g04347v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106866656"/>
    <dbReference type="Gramene" id="KQJ86265">
        <property type="protein sequence ID" value="KQJ86265"/>
        <property type="gene ID" value="BRADI_4g04347v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:106866656"/>
    <dbReference type="eggNOG" id="ENOG502QQPF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_43_5_1"/>
    <dbReference type="OMA" id="FEFRSAY"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:001055">
        <property type="entry name" value="Wall-associated receptor kinase-like 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF273">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095289" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="30"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="308"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="438"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ86265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="752" mass="80360" checksum="11E79DC8C3AEC2AA" modified="2012-06-13" version="1">MEHRTLLLPLAVVVTTLLVSTTWQQLASAAECQRRCGGVDIPYPFGIGRGCFLESPDMAFSLTCNLTAGDGNTSTRRPFSGALELLDVRLGRGQVRVRNRISSWCGHNGTPSMASGGSQLLPSSFRVSDAANRFTVIGCDALAYVGADADNGVIGMYSVGCRSMCPSAARLANGSSCSGMGCCQAAVPPGLSRYQVWFEDELNSSSSGRGAMSRPCSYAVLVEAAAFEFRTTYVTTGEFMEATGGQVPLVLDWVAGNQTCLEAKTQAAGYKCLSGNSECVDSRNGPGYLCNCSAGYQGNPYVLNGCQDIDECEQTGAAYPCSAPDTCSNTAGGFRCLCPAGHSGNAYTGPCEQKRSERPWQAAIGVSVGVVVLAVGMSCTYAAQEKRRLAAIKARHFRQHGGLLLFEEMKNKGNNGMVSSSSFTLFTREELREATGGFDERHVLGRGGNGTVYRGTLRDGTAVAIKRCRAAADGIDDDDGGRRQREFGKETLILSQINHKNIVKLYGCCLEVEVPMLVYQFIPNGTLYHLLHGGSDNNGESAAVPFAVRLRIAHETAEALAYLHSMASPPVIHGDVKSPNILLDGAYAAKVSDFGAATLAPPTDEAHLVTFVQGTCGYLDPEYMQTCRLTEKSDVYSFGVVLLELLTSRKALNLAAPDDERSLAASFLSAARDGRLDGLLDARVKGEVEAEVLEMVAGIAKMCLEMSGERRPSMRVVAEELDRIRKMSSSALQRSCLDEAQAHSVLDGISDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IKV9</accession>
    <name>I1IKV9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100833609</name>
        <name evidence="6" type="ORF">BRADI_4g15337v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of target mRNAs.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575930.1">
        <property type="nucleotide sequence ID" value="XM_003575882.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757573.1">
        <property type="nucleotide sequence ID" value="XM_014902087.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757574.1">
        <property type="nucleotide sequence ID" value="XM_014902088.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318631.1">
        <property type="nucleotide sequence ID" value="XM_024462863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IKV9"/>
    <dbReference type="SMR" id="I1IKV9"/>
    <dbReference type="EnsemblPlants" id="KQJ88097">
        <property type="protein sequence ID" value="KQJ88097"/>
        <property type="gene ID" value="BRADI_4g15337v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ88098">
        <property type="protein sequence ID" value="KQJ88098"/>
        <property type="gene ID" value="BRADI_4g15337v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833609"/>
    <dbReference type="Gramene" id="KQJ88097">
        <property type="protein sequence ID" value="KQJ88097"/>
        <property type="gene ID" value="BRADI_4g15337v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88098">
        <property type="protein sequence ID" value="KQJ88098"/>
        <property type="gene ID" value="BRADI_4g15337v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833609"/>
    <dbReference type="eggNOG" id="KOG2049">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004017_3_0_1"/>
    <dbReference type="OMA" id="SQYFGNW"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IKV9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000237">
        <property type="entry name" value="Pumilio homolog 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF147">
        <property type="entry name" value="PUMILIO HOMOLOG 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PUM-HD" evidence="5">
        <location>
            <begin position="415"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="440"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="476"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="515"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="588"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="624"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="660"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="696"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00058490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ88097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="767" mass="85319" checksum="1D43DC85362A70BB" modified="2012-06-13" version="1">MEDSQSNQDGCKFGAFVDGTPGAALGNAGDMELHCMGGGTSEAGRASLKMRRPLPSSPFTMYDTSTIGSNRSAVGFSHSDSLPDEQSLASAFGDMSFMDHTTDSPTTPTNFVPVSGYYPSGLTPPNVTLNLPIVPAIVQDDLVPLQFSAEHVNQKPNQLTLEHQEQAHRYPLHFGYFPTNSQMENLDRVFRPRYQPPALSASPIEQQFYIDRQSKIYAPYGQQFGSNFMSHHGMEEQTYPTIPPHYVYPQLEQVARSDVRRSRRHRQAAVCTSANGASHIGTPNTHRLGMGNDDPYSNGAAFLKRNNQLHNTYVNSFPGTLYADSSCDSGDFSQQDDKFSHPYALNSLSNGFSEDQISDDLSAASYPEKVLMRSDGVTSARSTKFAPSMNGCIGRDRRFSNEHNHLDNQSNDSSRLDRLHSEFLSLVMQSREPNYNSVDEVAGRIYMLAKDQNGCRFLQKVFAQGSQEDVEKVFGEIIDHIGDLMVDPFGNYLVQKLLEGCSEDQRMRILCEVTKMPGQLIAVSCNMHGTRAVQKIIETINSPDQVSKVVSALSPGAMHLMLDPNGSHVANRCLQKLLPESKVFLLDAATLHYLELATHQQGCCIIQKCIEHSNDEQKYSLLSNIISSALTLSDDQFGNYVIQSILNHNIGWATCKIVDELEGHFGYLSMQKCGSHVVENCLRQAPQHKRDRIIGELMNDPKLPHIMVDQFGNFVIQTALEHCKGTLHTAFVEAIRPHAAAMQSHMYGKRVLSKTYLKNKQHRVVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMJ2</accession>
    <name>I1IMJ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="6">M1Pi</shortName>
            <shortName evidence="6">MTR-1-P isomerase</shortName>
            <ecNumber evidence="6">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100834960</name>
        <name evidence="7" type="ORF">BRADI_4g22150v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="6"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577698.1">
        <property type="nucleotide sequence ID" value="XM_003577650.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IMJ2"/>
    <dbReference type="FunCoup" id="I1IMJ2">
        <property type="interactions" value="2485"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IMJ2"/>
    <dbReference type="EnsemblPlants" id="KQJ88931">
        <property type="protein sequence ID" value="KQJ88931"/>
        <property type="gene ID" value="BRADI_4g22150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834960"/>
    <dbReference type="Gramene" id="KQJ88931">
        <property type="protein sequence ID" value="KQJ88931"/>
        <property type="gene ID" value="BRADI_4g22150v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834960"/>
    <dbReference type="eggNOG" id="KOG1468">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_1_3_1"/>
    <dbReference type="OMA" id="CETRPLN"/>
    <dbReference type="OrthoDB" id="2461at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00874"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000002">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000003">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004326">
        <property type="entry name" value="PRK05720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ88931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="368" mass="38988" checksum="0F38B6513AC32779" modified="2012-06-13" version="1">MGESTSALQSILYDRGSLRLLDQRKLPLEEVYIDVKDSTDGWNAIRDMVVRGAPAIAIAAALSLAVEVFNQDFIGTSEEAASFVSKKLEYLVSSRPTAVNLSDAATKLQNLVLRTAETAKDAKSIFQVFIEATETMLVDDVADNKAIGLHGAEFLQRQVGRSKNISVLTHCNTGSLATAGYGTALGVIRALHSGGVLEKAFCTETRPFNQGSRLTAFELVHDKIPATLIADSAAAALMNNGQIQAVIVGADRIAANGDTANKIGTYNLSISAKHHGVEFYVAAPVTSIDLSLASGKQIVIEERSPKELLNSEGGLGKQVAASGISVWNPAFDVTPANLITAIITEKGVITKSDPNGTFDIKGFIKSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1INF7</accession>
    <name>I1INF7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100825763</name>
        <name evidence="4" type="ORF">BRADI_4g25410v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577831.1">
        <property type="nucleotide sequence ID" value="XM_003577783.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237937.1">
        <property type="nucleotide sequence ID" value="XM_010239635.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237938.1">
        <property type="nucleotide sequence ID" value="XM_010239636.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INF7"/>
    <dbReference type="STRING" id="15368.I1INF7"/>
    <dbReference type="EnsemblPlants" id="KQJ89387">
        <property type="protein sequence ID" value="KQJ89387"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89388">
        <property type="protein sequence ID" value="KQJ89388"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89389">
        <property type="protein sequence ID" value="KQJ89389"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89390">
        <property type="protein sequence ID" value="KQJ89390"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89391">
        <property type="protein sequence ID" value="KQJ89391"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89392">
        <property type="protein sequence ID" value="KQJ89392"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89393">
        <property type="protein sequence ID" value="KQJ89393"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT64170">
        <property type="protein sequence ID" value="PNT64170"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825763"/>
    <dbReference type="Gramene" id="KQJ89387">
        <property type="protein sequence ID" value="KQJ89387"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89388">
        <property type="protein sequence ID" value="KQJ89388"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89389">
        <property type="protein sequence ID" value="KQJ89389"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89390">
        <property type="protein sequence ID" value="KQJ89390"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89391">
        <property type="protein sequence ID" value="KQJ89391"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89392">
        <property type="protein sequence ID" value="KQJ89392"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89393">
        <property type="protein sequence ID" value="KQJ89393"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64170">
        <property type="protein sequence ID" value="PNT64170"/>
        <property type="gene ID" value="BRADI_4g25410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825763"/>
    <dbReference type="eggNOG" id="ENOG502RBI2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023710_0_0_1"/>
    <dbReference type="OMA" id="FSTFDCI"/>
    <dbReference type="OrthoDB" id="1925259at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1INF7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032795">
        <property type="entry name" value="DUF3741-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025486">
        <property type="entry name" value="DUF4378"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46836">
        <property type="entry name" value="AFADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46836:SF6">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14309">
        <property type="entry name" value="DUF4378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14383">
        <property type="entry name" value="VARLMGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3741" evidence="3">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4378" evidence="2">
        <location>
            <begin position="614"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ89387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="786" mass="88811" checksum="E1349312E848F754" modified="2012-06-13" version="1">MEQISVGHRYKECRPRRSSSCPTKLLVGKDVLKELEQRRSSPSVIAKLMGIDVLPPTYVANNRHQEFKDVFEVSEEPPEAVTKERSHPFPKGLPSLKRSAMRFKRLMPSKSPYGDGTFDNDLEYRNGIHHLNSLEIDNPLFEKRPHDVNYSPNHQHERDTASTFRKYPVGLANSSLRDIRNSSRQDYGGFNDIVVLEPGLRNCHDQENSFSMPFFPDVNKNSRRDKKQKQAEFAVMSNQRVSQHFLDAEDVNLARIKKERHFTVNAINPLSKGQEPSFDQFNMADLNSTGSSQRYASGDVNYRKNNMSSSNSLRWKIFRKYEQGDVGSKTLAEMFALSDSERVRRNPDSHAQVEQNKLDQGTGHSKEGCFIVLPTHAPPLPIHSSLDKSSSGGGSPYSENFSNISCNSSKFHVDSLRAKPSLKQIARDSQSNMRNASIVKNIALEQWGPASSSPSLDESRSHSWRLADNISASDCINEKVLFATDEDLVLEPAETVTSAFHSPLSKERKVSAMPFQRHDYDSITISNPVDAPKACKSSEEFQQPSPVSILEPPTDEDSYCSGFFMPALQDMPNVRNRISNCRLRDEPEVSTSSDDDNDSSYQSLEAFQVEEDRDFSYLLEILISSGMIVADWQLLCKSWYSPGFPVGPQVFDRLERKYNKVTTWSKPERLLLFDLANSVLSDVLAPCTDMHPWVSSRRQCSPIWGPEGPVEKVWQMMVRQQAELGIGHPDDKVLDPNWLEVGDDINMVGKQIARMLYADLLGEVVTEFIAGSVVSPRSNSQPPMYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQ69</accession>
    <name>I1IQ69_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100842080</name>
        <name evidence="11" type="ORF">BRADI_4g30470v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578133.1">
        <property type="nucleotide sequence ID" value="XM_003578085.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQ69"/>
    <dbReference type="SMR" id="I1IQ69"/>
    <dbReference type="FunCoup" id="I1IQ69">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IQ69"/>
    <dbReference type="EnsemblPlants" id="KQJ90267">
        <property type="protein sequence ID" value="KQJ90267"/>
        <property type="gene ID" value="BRADI_4g30470v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842080"/>
    <dbReference type="Gramene" id="KQJ90267">
        <property type="protein sequence ID" value="KQJ90267"/>
        <property type="gene ID" value="BRADI_4g30470v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842080"/>
    <dbReference type="eggNOG" id="KOG0001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_0_0_1"/>
    <dbReference type="OMA" id="MMADYGI"/>
    <dbReference type="OrthoDB" id="1649877at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019941">
        <property type="term" value="P:modification-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01806">
        <property type="entry name" value="Ubl_NEDD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000023">
        <property type="entry name" value="NEDD8 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000016">
        <property type="entry name" value="Polyubiquitin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038738">
        <property type="entry name" value="Nedd8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ90267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="153" mass="17130" checksum="1929C1B3AEF7CAC9" modified="2012-06-13" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGTMIKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITX3</accession>
    <name>I1ITX3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-ascorbate peroxidase</fullName>
            <ecNumber evidence="4">1.11.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100826086</name>
        <name evidence="19" type="ORF">BRADI_4g41180v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">Bd21</strain>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-ascorbate + H2O2 = L-dehydroascorbate + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:22996"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.11.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.11" evidence="4"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578843.1">
        <property type="nucleotide sequence ID" value="XM_003578795.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITX3"/>
    <dbReference type="STRING" id="15368.I1ITX3"/>
    <dbReference type="EnsemblPlants" id="KQJ92013">
        <property type="protein sequence ID" value="KQJ92013"/>
        <property type="gene ID" value="BRADI_4g41180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826086"/>
    <dbReference type="Gramene" id="KQJ92013">
        <property type="protein sequence ID" value="KQJ92013"/>
        <property type="gene ID" value="BRADI_4g41180v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826086"/>
    <dbReference type="eggNOG" id="ENOG502QS7Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036959_2_1_1"/>
    <dbReference type="OMA" id="PIKEKHA"/>
    <dbReference type="OrthoDB" id="2859658at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016688">
        <property type="term" value="F:L-ascorbate peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00691">
        <property type="entry name" value="ascorbate_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000007">
        <property type="entry name" value="L-ascorbate peroxidase S chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000005">
        <property type="entry name" value="L-ascorbate peroxidase T, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044831">
        <property type="entry name" value="Ccp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002207">
        <property type="entry name" value="Peroxidase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356:SF1">
        <property type="entry name" value="L-ASCORBATE PEROXIDASE S, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00459">
        <property type="entry name" value="ASPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="15" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="18">
        <location>
            <begin position="68"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="208"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQJ92013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="314" mass="34435" checksum="540E7CFA84326571" modified="2012-06-13" version="1">MAVHHHLLRRGLAGGSPLSPLRGLFLVSQELGRRQASSAAAGDAAAELRGAREDVRQLLKDKHCHPILVRLGWHDSGTYDKNISEWPKCGGANGSLRFEIELKHAANAGLVNALKLVQTIKDKYAGVTYADLFQLASATAIEEAGGPKIPMIYGRVDVSAPEQCPPEGRLPAAGPPSPAEHLREVFYRMGLNDKEIVALSGAHTLGRSRPERSGWGKPETKYTKNGPGATGGQSWTSQWLKFDNSYFKDVKERRDEDLLVLPTDAVLFEDPSFKIYAERYAEDQDTFFEDYAEAHAKLSNLGSKFDPPKGISLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IXA8</accession>
    <name>I1IXA8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100835804</name>
        <name evidence="7" type="ORF">BRADI_5g08750v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581167.1">
        <property type="nucleotide sequence ID" value="XM_003581119.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IXA8"/>
    <dbReference type="STRING" id="15368.I1IXA8"/>
    <dbReference type="EnsemblPlants" id="KQJ82395">
        <property type="protein sequence ID" value="KQJ82395"/>
        <property type="gene ID" value="BRADI_5g08750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835804"/>
    <dbReference type="Gramene" id="KQJ82395">
        <property type="protein sequence ID" value="KQJ82395"/>
        <property type="gene ID" value="BRADI_5g08750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835804"/>
    <dbReference type="eggNOG" id="KOG1100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038018_1_0_1"/>
    <dbReference type="OMA" id="TESHSWQ"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42647:SF64">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42647">
        <property type="entry name" value="SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="264"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ82395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="311" mass="32741" checksum="2AF4D02BE8ED5563" modified="2012-06-13" version="1">MAVQAQHAFHHDLHSYRALEDGTTGASLFLDTPAVAGVVNTAVLSDLTCNDHSYDYAFAPRKRARVAAEAPGFAVELEQPRGVRTPAVPQEFVPVGDMLSRVVGSGTPSTSGRIGNAAGVPRLFHPGMDIDALVRVETERMRACLEEARRRHVRALVAAAERATAGRLRAAESALELARGRTAELEERLRQTIAEGQAWIGVARSHEAVAAGLRDTPDQLLIQSPSCAAQSGECEDAQSCCFETTPACADDGDAASMASAACCCKACGEGGACVLLLPCRHLCLCRACEGAVDTCPVCAATKNASFHVLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IYE1</accession>
    <name>I1IYE1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Endoplasmic reticulum vesicle transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100841488</name>
        <name evidence="8" type="ORF">BRADI_5g12110v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579883.1">
        <property type="nucleotide sequence ID" value="XM_003579835.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IYE1"/>
    <dbReference type="STRING" id="15368.I1IYE1"/>
    <dbReference type="EnsemblPlants" id="KQJ82928">
        <property type="protein sequence ID" value="KQJ82928"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ82929">
        <property type="protein sequence ID" value="KQJ82929"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ82932">
        <property type="protein sequence ID" value="KQJ82932"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ82934">
        <property type="protein sequence ID" value="KQJ82934"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61210">
        <property type="protein sequence ID" value="PNT61210"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61211">
        <property type="protein sequence ID" value="PNT61211"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841488"/>
    <dbReference type="Gramene" id="KQJ82928">
        <property type="protein sequence ID" value="KQJ82928"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82929">
        <property type="protein sequence ID" value="KQJ82929"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82932">
        <property type="protein sequence ID" value="KQJ82932"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82934">
        <property type="protein sequence ID" value="KQJ82934"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61210">
        <property type="protein sequence ID" value="PNT61210"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61211">
        <property type="protein sequence ID" value="PNT61211"/>
        <property type="gene ID" value="BRADI_5g12110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841488"/>
    <dbReference type="eggNOG" id="KOG2667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="QRHEGCR"/>
    <dbReference type="OrthoDB" id="270930at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IYE1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF82">
        <property type="entry name" value="ENDOPLASMIC RETICULUM VESICLE TRANSPORTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="6">
        <location>
            <begin position="149"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ82928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="43447" checksum="E542591B37FF9832" modified="2012-06-13" version="1">MDGLMSKLRNLDAYPKVNEDFYSRTLSGGVITLASSFVMLLLFVSELRLYLHAVTETTLRVDTSRGEKLRINFDITFPALQCSIISIDVMDISGQEHLDVKHDVFKQRIDANGNVIATKQDAVGGMKVEKPLQMHGGRLEHNETYCGSCYGAEEPGEQCCNSCEDVREAYRKKGWGVSNPDSIDQCKREGFLQTIKDEEGEGCNIYGFVEINKVAGNFHFAPGKSFQQSNVHVHDLLPFQKDSFNVSHKINKLSFGEPFPGVVNPLDGAHWFQHSPYGMYQYFVKVVPTVYSHINEQIILSNQFSVTEHARSSESVRMQALPGVFFFYDLSPIKVTFTERHVSFLHFLTNVCAIVGGVFTVSGIIDSFVYHGQRAITKKREIGKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1J1F2</accession>
    <name>I1J1F2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Thylakoid lumenal 29 kDa protein, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">p29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100831503</name>
        <name evidence="17" type="ORF">BRADI_5g20670v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-ascorbate + H2O2 = L-dehydroascorbate + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:22996"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.11.1.11"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 15">Belongs to the peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240367.1">
        <property type="nucleotide sequence ID" value="XM_010242065.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J1F2"/>
    <dbReference type="FunCoup" id="I1J1F2">
        <property type="interactions" value="1475"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J1F2"/>
    <dbReference type="EnsemblPlants" id="KQJ84404">
        <property type="protein sequence ID" value="KQJ84404"/>
        <property type="gene ID" value="BRADI_5g20670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831503"/>
    <dbReference type="Gramene" id="KQJ84404">
        <property type="protein sequence ID" value="KQJ84404"/>
        <property type="gene ID" value="BRADI_5g20670v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QR5T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="EAYTYPR"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J1F2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009543">
        <property type="term" value="C:chloroplast thylakoid lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016688">
        <property type="term" value="F:L-ascorbate peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00314">
        <property type="entry name" value="plant_peroxidase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000013">
        <property type="entry name" value="thylakoid lumenal 29 kDa protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1620:FF:000001">
        <property type="entry name" value="Thylakoid lumenal 29 kDa protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044831">
        <property type="entry name" value="Ccp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002207">
        <property type="entry name" value="Peroxidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356:SF34">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00459">
        <property type="entry name" value="ASPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="9" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="16">
        <location>
            <begin position="116"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ84404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="366" mass="39325" checksum="3F60910ADCCF4B14" modified="2012-06-13" version="1">MRWSSKGGESMAGATFLSAPALLGPRAGGLPSVSVTSSPARRQHAYIQVCCHAKAGGSEAAAAAREEPPRFRRRDLIGGCMGTAVGLEIIDGSTGVATAADLIERRQRSEFQSSIKGTLTAAIQAKPELVPSLLTLALNDAMTYDKATKSGGPNGSIRLSAEINRPENSGLSAALDMLTDAKKEIDSYSKGGPIAFADLIQIAAQQALKQTFLDAAIAKTGGNEEKGRTLYSAYGSSGQWGLFDKIFGREDTQEPDPEGRVPKWSSASVQEMKHKFIAVGFGPRQLAVMSAFLGPDQAATEAKLIADPDCRPWVEKYQRSRETVSRTDYEVDLITTLTKLSYLGQKINYEAYTYPKQKINLGKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E3L6</accession>
    <name>A0A0Q3E3L6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g07715v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E3L6"/>
    <dbReference type="EnsemblPlants" id="KQJ82238">
        <property type="protein sequence ID" value="KQJ82238"/>
        <property type="gene ID" value="BRADI_5g07715v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82238">
        <property type="protein sequence ID" value="KQJ82238"/>
        <property type="gene ID" value="BRADI_5g07715v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E3L6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ82238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="152" mass="17806" checksum="E8197A0AD1FD20AD" modified="2016-01-20" version="1">MLPIRFVELMWMCSIWSSGLQLTCQFIYMVLPPSMFIRATSTMGITWVPRKSTSQCARRTHITSTCLSNHHYMGTKKKEKMTCGSIKKEKGIRKEKNILKREEKKISIDHHWRCPRPHLAISSTKTNSMRFKEVRDFRFGLGPKRLVINANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E9Q0</accession>
    <name>A0A0Q3E9Q0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2-oxoglutarate-dependent dioxygenase DAO</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein DIOXYGENASE FOR AUXIN OXIDATION</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100840988</name>
        <name evidence="12" type="ORF">BRADI_5g13320v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">2-oxoglutarate-dependent dioxygenase essential for auxin catabolism and maintenance of auxin homeostasis in reproductive organs. Catalyzes the irreversible oxidation of indole-3-acetic acid (IAA) to the biologically inactive 2-oxoindole-3-acetic acid (OxIAA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579963.1">
        <property type="nucleotide sequence ID" value="XM_003579915.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E9Q0"/>
    <dbReference type="FunCoup" id="A0A0Q3E9Q0">
        <property type="interactions" value="1603"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3E9Q0"/>
    <dbReference type="EnsemblPlants" id="KQJ83140">
        <property type="protein sequence ID" value="KQJ83140"/>
        <property type="gene ID" value="BRADI_5g13320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840988"/>
    <dbReference type="Gramene" id="KQJ83140">
        <property type="protein sequence ID" value="KQJ83140"/>
        <property type="gene ID" value="BRADI_5g13320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840988"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3E9Q0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009852">
        <property type="term" value="P:auxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000017">
        <property type="entry name" value="2-oxoglutarate-dependent dioxygenase DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="11">
        <location>
            <begin position="147"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ83140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="298" mass="32440" checksum="C78FBEE61E438651" modified="2016-01-20" version="1">MVEIPVIDLRLAGAAPEESARLRDACERLGCFRVFGHGVPAALQADMKAALRALFDLPDDAKRRNTEIIAGSGYVPPSAANPLYEAFGLWDAAVPADVDAFCARLDAPPHAREAVKSYAEKMHELIVDVAGKVAASLGLEGHPFQDWPCQFRMNRYNYTEDTVGSSGVQIHTDSGFLTVLQEDDCVGGLEVLDPAAGEFVPVDPFPGSFLVNIGDVGTAWSNGRLHSVKHRVQCVAAVPRISIAMFLLAPKDDRVCTPEAFVDADHPRRYRAFNYDEYRKLRLSTGERAGEALARMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EDZ8</accession>
    <name>A0A0Q3EDZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100838760</name>
        <name evidence="12" type="ORF">BRADI_5g22146v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3EDZ8"/>
    <dbReference type="EnsemblPlants" id="KQJ84668">
        <property type="protein sequence ID" value="KQJ84668"/>
        <property type="gene ID" value="BRADI_5g22146v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ84668">
        <property type="protein sequence ID" value="KQJ84668"/>
        <property type="gene ID" value="BRADI_5g22146v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="689569at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EDZ8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF73">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="34"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="310"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="577"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="11">
        <location>
            <begin position="824"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="99"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ84668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1523" mass="171473" checksum="56FC22AEC9F11F6F" modified="2016-01-20" version="1">MASPEDTSLEPQIGWLAETILGTLQIEKLDTWIRRAELDDVVQKLKSEVERVEMVVAAVRGRATGNKPLSRSLARLKDLLYDADDAVDELDYYRLQQQVERDPIHNPESTHGAEAERVDETSRGAAAAADIPNSSGGHLRSKHWKDFTVITWESGKAVEAKCNLCGAHPIKCGSGNGTSVLSNHLKSKGCIRKRRGPPNPSSTDDATEIARPIVIGDSSSGKRKRADHESSKFTATNTDTPWDKAELSNRIQKITSQLQDIRGKVSEILKIHGPDFPSSSNNHRIAASDHHLRTTSSLAPRKVYGRVAEKKAIIKMIMQNKSNGVTVLPIVGTAGVGKTTLAQLVYNDPDVESHFDTKLWLRVSHNFDEVRLTREMLDFVPQDRCVGSLHINERHEGISSFAKLQEILKERMEYQLKRFLLILDDVWDNLDDERWNKLLNPLISSQAKGNVILVTTRNFCVAKKKGTLEPIQLCALEDEDFLLLFKSRAFGDENYKGDPSLSLIGRQIAEKLAGNPLAAETAGELLREHLTIDHWTKILKDEHWKSLQLSTGIMCALRLSYDQLPYHIQQCFSYTSIFPRSYRFPGEMLVHIWISQGFVNGNQSSKRLEEIGRHNLTVLVNLGFIQQVEIFLSCQPCYAICGLMHHFGRIVSRTECAIIDGLQCNEMLPTVLHLSIVTDYAYNKDQDGEFSRNELFEKTLNNTVTSVSKLRSLVLLGHYDPCFLQLFQRKFQEAQNLRLLQMSVTSVDFSSFQCSFVKPAHLRYLKLGSVEVRGDFPRVFSKLYQLQVLDVGLYAHPTAPNGIHNLVSLRLLDFKVQTSSGSEITQLQSMNELVQLGVSQLDNVKSSEEAYGAGLRNKGHLENLHLSWKDTLSDNESPADTARKHSEVLEGLKPHKHLKHLQISGYNGTSPTWLNSNMSITSLQTLHLDCCREWEILPSLERFPCLRKLKLNNMRKVTKILVPSLEELVLVDMPELERCSCTSLEGFNSSLRSLRIENCEKLEVFDLFENAGKFKVEHRSWLSGVRELILLDCHLLKVFSHLPPSATFSELQIRGVSTLPSMNGSYEKLHIESDWEDPSTECVGEVLAFHNLRSLKFLSINGYGVNSMSIFFKDLSHLVSLKSLEIARCGIVFSSYVIPEPTCEDVLAANRKLFPSLQSLTVESCRITGKGLSLMLQHSPVLEKLDLFDCIGITLLTVDEEENSLSNLISDRETQDELLHIPFNVSSTLKELSFVDFHCLRFNGSKKGFSGFTSLENLHIWDCPELLSSLVRKDGSDDQANGRWLLPESLGELDIVSYPEESLQRCFPNNLNSLKKLVLWNADLKSLQLHSCTGLEELEFAGCDSLSIVEGLQSLGSLRDLTVLGCPCLPSYLESFSRQCNELLPRLETLVINDPGVLTTSFCKHLTSLHDLKLVWMKVTRLTEEQERALVLLKSLQELTFDGCHHVMHLPAGLHTLPSLKRLKIDSCSSILWQPETGFPDSLEELELLRCSKELDDECMLLATPMSKLKEEVSVPEMLFSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F662</accession>
    <name>A0A0Q3F662_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100833898</name>
        <name evidence="17" type="ORF">BRADI_3g15620v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571415.2">
        <property type="nucleotide sequence ID" value="XM_003571367.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F662"/>
    <dbReference type="STRING" id="15368.A0A0Q3F662"/>
    <dbReference type="EnsemblPlants" id="KQJ95183">
        <property type="protein sequence ID" value="KQJ95183"/>
        <property type="gene ID" value="BRADI_3g15620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833898"/>
    <dbReference type="Gramene" id="KQJ95183">
        <property type="protein sequence ID" value="KQJ95183"/>
        <property type="gene ID" value="BRADI_3g15620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833898"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3F662">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08640">
        <property type="entry name" value="DNA_pol_A_plastid_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000034">
        <property type="entry name" value="DNA polymerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000051">
        <property type="entry name" value="DNA polymerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.10">
        <property type="entry name" value="Taq DNA Polymerase, Chain T, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="DNA POLYMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133:SF27">
        <property type="entry name" value="DNA POLYMERASE NU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="16">
        <location>
            <begin position="757"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="577"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="640"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="583"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ95183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1026" mass="115350" checksum="A69DD50841131682" modified="2016-01-20" version="1">MAAAAGPGPWPAHLRPRCPCSSPPWLSPNLSRRRWPVPPAFPGPRRRDYSNSSGMGIKERGDTILGFFANLNMQSNEQEWVKESRRIFYLRRTNNVRNNVYNGATDLRAGNSNRQPLEDHSSPDCPYLYDIRETLPSKSIVDRHADTGLAKSSMTNQSAHAVSTVMSVVNADIKTLCIPSSSKGDISLHDCSSMEAPLPTISNTEATLGFDDKANVRDENEDRLIAEKVAPSIPANIFLTNEPKSARKALAGIYDKVLVVDSIESAKNVVQLLTTKYKNFVHACGTEVTNIDVKEETPVDHGEVICFSIYSENSGSQADFGNGKTCIWVDVLDGQRDVLMEFAPFFEDPSIKKVWHNYSFDSHVIENCRIKVDGFHADTRHLARLWNSNRKTDGGYSLEGLSNDHRVMTDVPKDLSEIGKASKKTIFGKKKIRKDGAEGKIVNVEPVEKLQREDRNMWICYSSRDSMNTLKLYESLKSKLEAKEWIFDGCPRGTMYDFYEEYLRPFGALLVKMEREGMLVDRGYLSEIEKIAVAERNLAADKFRKWASKYCPDAKYMNINSDAQILQLFFAGRENRDTPGETLPQSKTFRVPNDQSVAPEGKRTSKFRTIKLCGIVENPEVTFSSSVSLDTLKTWPGKIPTDQIYRTDDDQEHDEYSNGSELPDQDVEEVSLYGTAYEVFGGGKKGKEACHAIAALFETRSIAKLISSFIVPLQEDHISCKEGRIHCSLNINTETGRLSARTPNLQNQPALEKDRYKIRQAFVAAPGNSLVVADYGQLELRILAHLTNCKSMLDAFKAGGDFHSRTAMNMYEHIRDAVHEKKVLLEWHPQPGQEKPPVPLLKDAFGAERRKAKMLNFSIAYGKTAKGLSDDWKVTVDEARDTLKLWYRDRKEVSAWQKRQKELVREKCEVYTLLGRSRHFPNMTHAGSSKKGVERAAINAPVQGSAADVAMCAMLEIERNVRLKELGWRLLLQVHDEVILEGPTESAETARAIVVECMSKPFYGTNILKVELAVDAKCVKSWYAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FKP0</accession>
    <name>A0A0Q3FKP0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100830652</name>
        <name evidence="2" type="ORF">BRADI_3g45410v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235518.1">
        <property type="nucleotide sequence ID" value="XM_010237216.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235519.1">
        <property type="nucleotide sequence ID" value="XM_010237217.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FKP0"/>
    <dbReference type="FunCoup" id="A0A0Q3FKP0">
        <property type="interactions" value="900"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99821">
        <property type="protein sequence ID" value="KQJ99821"/>
        <property type="gene ID" value="BRADI_3g45410v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99822">
        <property type="protein sequence ID" value="KQJ99822"/>
        <property type="gene ID" value="BRADI_3g45410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830652"/>
    <dbReference type="Gramene" id="KQJ99821">
        <property type="protein sequence ID" value="KQJ99821"/>
        <property type="gene ID" value="BRADI_3g45410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99822">
        <property type="protein sequence ID" value="KQJ99822"/>
        <property type="gene ID" value="BRADI_3g45410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830652"/>
    <dbReference type="OrthoDB" id="765741at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FKP0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040276">
        <property type="entry name" value="At4g26450-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36056:SF2">
        <property type="entry name" value="OS02G0550000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36056">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ99822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="841" mass="93417" checksum="5BCB7D3E091C3AEC" modified="2016-01-20" version="1">MQYHHRSRLPPPPPPPPFGRGGGAVYPRGHRQLYAPPPPRPPLPAPPPPPQRRYEVLMEAGRLAAEYLVSTGVLPPSSLHRGSGDAWTVPPPPPPPMSIPPPPLQKQQEPPAFYGRRRYENVYSNNNPGSRPRRNSNTSSSGSRDDYSNGGSYNGRGKRKYGEYRRGYSDWGRDREKERGRSSSNGRRHEEDDDEDGAPGFRRDRRGSGGNDEARSCVTEEIREATPLMVKDVVDMEMEDIRSNAVSSSDDVRKDADALSAVHTENEEGETKENKVSSSESEVVEQGVDTNGDVSKVMEAERKLLLDGKVVDEKAEDNGKVLCETSLDRITLDDDATNLENGLHVDRRNLLQHCGFAKAPTRPRSVRAHRNIVTETANRVFSGEASQMAVNEVGEEKLLTNIQSDSREDEFHQENNGSSAACNETVAPMPLQENRTSDVTENMREDKNNAQLHLVQQHKEETDVSSLTTAQKDSFMQENDMSPLTASREDVMIQETNLSMLTDTHEDSLIEETNLSPKIKFQSHSLIEETNLSPSIDVTDQHKDSFIKETDLSPKIKFQSHSLIEETNLSPLIDVTDQHKDSFIKETDLSPLTASHKVSLMQETALPPIMSSDEHNLNLQFKEGSQICDIEILPQDVDLIELSHQRDIAGAELLSIVEAEDIIKMEEEKLDQSRSFEIHNNPGLGQSSAQVSSADPHKLLQGDFGASKLEEMNPELCQASLDSKAVQVIGIEDETNIEVAGFDSSKAKNEMICSSMDDLLHPGIHTDELPVIQDGYNFTLSDYLGDDIPCYTSMQPDLQAGICSNDSEGIASMDDSIYGSLTDIGFMDVWDQPAEDFAKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FYR2</accession>
    <name>A0A0Q3FYR2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BAH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100841016</name>
        <name evidence="4" type="ORF">BRADI_2g14070v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316015.1">
        <property type="nucleotide sequence ID" value="XM_024460247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FYR2"/>
    <dbReference type="EnsemblPlants" id="KQK04528">
        <property type="protein sequence ID" value="KQK04528"/>
        <property type="gene ID" value="BRADI_2g14070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841016"/>
    <dbReference type="Gramene" id="KQK04528">
        <property type="protein sequence ID" value="KQK04528"/>
        <property type="gene ID" value="BRADI_2g14070v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1922186at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FYR2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001217">
        <property type="term" value="F:DNA-binding transcription repressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140566">
        <property type="term" value="F:histone reader activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46871:SF3">
        <property type="entry name" value="BAH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46871">
        <property type="entry name" value="BROMO-ADJACENT HOMOLOGY (BAH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAH" evidence="2">
        <location>
            <begin position="151"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="3">
        <location>
            <begin position="360"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK04528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="61807" checksum="6545BA3B0456027E" modified="2016-01-20" version="1">MGKRRRPRMQYTSDDDDDDGGGGHNAAPEIPKTIKPTVVPDSSRPDKQQRRHTDELVEEGEHENDLVAARRNEEEERREDAHTLHRGGSRKPGRSAEDINEELKEKKAEGAEEEEEDEDGPDAVPVGKPVKVTDKGNAQRKHYDAFECEGNTYKLWDTAMFVPELENQKPYVGIIKDIHKIGGSLSVTAQWFYRPEEADEDGGEPRELFYSSHIDEVPAGSVMHTCMVHFIPQHKQVPSMKEHPGFIVQKVYDHIKEKLWDVADKDYLDNMQHEVDLLVKETMDRIGELPDRDPEDIPGSNADKFSSKFSGGLRKRPVNPKDGPTTGTSQQFVKADTPWNYNLNNYAILERYKAITGNPSRDRWIDKFVDTILTPCKNDTENSYPPNVVVQIVASLERSAFEAFYADFQKYNQKMRTLLFNIKENSVLRKQLMNKELDPVALLTMAPAELKAGLMPAEITPETEEPRQLQMTESRCKGCAEKNVGITELFLANRGGRYKVYIPNTCTRGNLQNYPMHGFRLECLSCGLSWFAFRDDISLRSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FZ59</accession>
    <name>A0A0Q3FZ59_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucosidebeta-D-glucosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_2g09187v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FZ59"/>
    <dbReference type="EnsemblPlants" id="KQK03661">
        <property type="protein sequence ID" value="KQK03661"/>
        <property type="gene ID" value="BRADI_2g09187v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03661">
        <property type="protein sequence ID" value="KQK03661"/>
        <property type="gene ID" value="BRADI_2g09187v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FZ59"/>
    <dbReference type="OrthoDB" id="65569at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FZ59">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF290">
        <property type="entry name" value="4-HYDROXY-7-METHOXY-3-OXO-3,4-DIHYDRO-2H-1,4-BENZOXAZIN-2-YL GLUCOSIDEBETA-D-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999554" description="4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucosidebeta-D-glucosidase" evidence="4">
        <location>
            <begin position="22"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK03661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="54079" checksum="53A00EB4413CC985" modified="2016-01-20" version="1">MRPLRRVVALLLAAFSVVGSAAPSPARSEGIISRDDFPAGFVFGAGTSAYQWEGAAAEDGRTPSVWDTHARAHAHGGDDPVNGDVAADGYHKYKEDIKLMKETGLDAYRFSISWSRLIPRIQPHVTMFHYDLPQILEDEYDGWLSPQIIGDFTAYADVCFREFGDRVTNWTTLNEPNALVALGYDSGIGPPGRCSKPFGDCSRGNSVDEPYIVAHNCLLAHSSAVSLYKRKYQAKQKGLIGINLYIYNILPFTNSTEDIAATKRARAFYTGWFLDPLYHGDYPLLMKENTGSKLPIFSQNQSEQLINSVDFLGINYYKIIYVKDDPQNGPINKSDYVADMSAKAILASDSTTGFHVLGFGLQEELEYLKQSYGNPPICIHENGYPMHQHVVFDDGPRVEFLSTHLRSLVISLRNGSNTRGYFVWSLMDMYELLSLRNTYGLYYVDFADKDLKRYPRSSAIWYANFLKGRRDSGCTERFSDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FZR0</accession>
    <name>A0A0Q3FZR0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_2g10311v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03869.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FZR0"/>
    <dbReference type="EnsemblPlants" id="KQK03869">
        <property type="protein sequence ID" value="KQK03869"/>
        <property type="gene ID" value="BRADI_2g10311v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03869">
        <property type="protein sequence ID" value="KQK03869"/>
        <property type="gene ID" value="BRADI_2g10311v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FZR0"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FZR0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932">
        <property type="entry name" value="ATPASE EXPRESSION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932:SF2">
        <property type="entry name" value="OS10G0484300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="86"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="122"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="192"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="228"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="263"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="298"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK03869.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="38155" checksum="3AC1400A4F58B315" modified="2018-03-28" version="2">MRFSLRRPHSRPSSSPLPATAAAAASCHRIAVLSTASPSYAASAGGAAFLPTVRLNRELARCARSCPVSALARFSRVLRAGSPPPDAATFNVLLQCLCLLGRLAAARRLFDAMRGLAPGLPSVVSCNTLAKGYCLGGRLTDVLDLLSWMLGAALQPNKRTYSIIIDGACGMGYPELGFGFLVSMLRGGLFPSVPTYSCLLAGLCTRGGNLKQALVLYGRMVKMGVRGNIVSYSGFLNALCRTEMVTQAEVLLGDMLVAGCPPNVVTCTALVKGLCTIGRVHHARIVIKHMVNLGIVPNVVTYTTLMSGLCKEGRMGEAVGLLARLGDAEQAAILAQEMSKKGIELDHIGHCILSQGMRRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G284</accession>
    <name>A0A0Q3G284_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GBF-interacting protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100825596</name>
        <name evidence="3" type="ORF">BRADI_2g15260v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230981.2">
        <property type="nucleotide sequence ID" value="XM_010232679.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G284"/>
    <dbReference type="STRING" id="15368.A0A0Q3G284"/>
    <dbReference type="EnsemblPlants" id="KQK04685">
        <property type="protein sequence ID" value="KQK04685"/>
        <property type="gene ID" value="BRADI_2g15260v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825596"/>
    <dbReference type="Gramene" id="KQK04685">
        <property type="protein sequence ID" value="KQK04685"/>
        <property type="gene ID" value="BRADI_2g15260v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="762072at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3G284">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009719">
        <property type="entry name" value="GIP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46445:SF8">
        <property type="entry name" value="OS05G0581800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46445">
        <property type="entry name" value="RNA POLYMERASE II DEGRADATION FACTOR-LIKE PROTEIN (DUF1296)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06972">
        <property type="entry name" value="GIP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBF-interacting protein 1 N-terminal" evidence="2">
        <location>
            <begin position="31"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="817"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="831"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK04685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="849" mass="89131" checksum="4B2C801DEECE17C7" modified="2016-01-20" version="1">MRAGGGGRGGGGGRQQQPRAAAAAAGEGAEIPPASRKLVQGLKGILADRTEAEIYATLLDCGMDPDVAVERLISQDPFHEVRRKRNNKKEVKAPQEARSRPFYKSTYRGSKAVSDRSGRSYSGLGADSTGSVKGTSKKEAGLIPPENLSASDSVKASNPTETVSAAGNLADAKPSTFQPPSQAQHGWGGVPGRPSMADIVKMGRPQAKSGSRSAGISAGVPTVAGSVVSNASNPIPKDSHNTVLPSEGDHVTANKLPNGTVQVHSVPADDSFVDILPPGEGSDVPESFGAVSTNAKPAGSIIPEVNEVDFGNDDNFEETKEMSASNASGLTSSGPLSVSDKDVPSSNDLIEKSDNCQSDKDALEHRQDSNDNMSTTSYLLEHLTIHEEKRPKASEDNPAVIIPGHLQVSNADFADLTFGSFVSGTLDVSCSTVPANSDGEVTSVSENHSSDQSDVRIHEYENKEAVIPAANEYIAPATNSNMENTSINSVQQSEVGRAGLMGVTNSAEYNLSPTDYATSSTVQPDSAPQNYLQENRQMQNISPLSSFMQGNIPNGLLPPAMPPFRELDPAFSLLLTNPPLATMIHGTPPSSMSNTTVSTQPQENVNPGGLSNTQLTHSQASTSMAPLPHHLALHPYAQATLPLGYTSMMGYPSLPQSYYLPPAAFQQPYMNSGLFHQAAAAAAAAAAPNSNVKYPMPQYKSNVPLASMPQQASLLSNYVGGFGTANGMPGNYALNQSSVPANAAPGFDGTMPSQYKDGNHYISLQQNENPAMWMHGAGSRGMPPLAANTLYGYQGQQGHQGGLRQGQLPSQFGAALGQSQQGLGQEHRNPSDSNLSAAGQANQMWPNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GNY7</accession>
    <name>A0A0Q3GNY7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome b6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BRADI_1g05768v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Thylakoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the cytochrome b family. PetB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GNY7"/>
    <dbReference type="FunCoup" id="A0A0Q3GNY7">
        <property type="interactions" value="384"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GNY7"/>
    <dbReference type="EnsemblPlants" id="KQK12751">
        <property type="protein sequence ID" value="KQK12751"/>
        <property type="gene ID" value="BRADI_1g05768v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12751">
        <property type="protein sequence ID" value="KQK12751"/>
        <property type="gene ID" value="BRADI_1g05768v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GNY7"/>
    <dbReference type="OrthoDB" id="770670at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009579">
        <property type="term" value="C:thylakoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00284">
        <property type="entry name" value="Cytochrome_b_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.810.10:FF:000001">
        <property type="entry name" value="Cytochrome b6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="entry name" value="Cytochrome Bc1 Complex, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00633">
        <property type="entry name" value="Cytb6_f_cytb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005797">
        <property type="entry name" value="Cyt_b/b6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023530">
        <property type="entry name" value="Cyt_B6_PetB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048259">
        <property type="entry name" value="Cytochrome_b_N_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002990">
        <property type="entry name" value="PRK03735.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271:SF38">
        <property type="entry name" value="CYTOCHROME B_B6 N-TERMINAL REGION PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00033">
        <property type="entry name" value="Cytochrome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000032">
        <property type="entry name" value="Cytochrome_b6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="Transmembrane di-heme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 N-terminal region profile" evidence="19">
        <location>
            <begin position="21"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KQK12751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="232" mass="26315" checksum="36DE545837570058" modified="2016-01-20" version="1">MKFSYTVLRGGLRLVTYFNKVYDWFEERLEIQAIADDITSKYVPPHVNIFYCLGGITLTCFLVQVATGFAMTFYYRTTVTEAFSWVQYIMTEANFGWLIRSVHRWSASMMVLMMILHVFRVYLTGGFKKPRELTWVTGVVLAVLTASFGVTGYSLPWDQIGYWAVKIVTSVPDAIPIIGSPLAELLRGSASVGQSTLTRFYSLHTFVLPLLTAVFMLMHFPMIRKQGISGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GUK4</accession>
    <name>A0A0Q3GUK4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BRADI_1g17330v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14567.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GUK4"/>
    <dbReference type="EnsemblPlants" id="KQK14567">
        <property type="protein sequence ID" value="KQK14567"/>
        <property type="gene ID" value="BRADI_1g17330v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14567">
        <property type="protein sequence ID" value="KQK14567"/>
        <property type="gene ID" value="BRADI_1g17330v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GUK4"/>
    <dbReference type="OrthoDB" id="640735at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999583" description="Subtilisin-like protease" evidence="11">
        <location>
            <begin position="21"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="13">
        <location>
            <begin position="42"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="144"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="14">
        <location>
            <begin position="613"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK14567.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="705" mass="74601" checksum="57B966426FBAA5BB" modified="2018-03-28" version="2">MVSLTNLFLPLLLLSTLTVAVNPTRATELVHQDVTGTSGYLTYIVLVEPPPTKVTEDERHRWYESFLPSSRVGESGEPRLIHSYTEVFDGFAARLTNAELSAVAKKPGFVRAFPDRKRQLMTTHTPKFLGLRNGTGFWSEARYGKGVIIGLLDTGIHATHPSFDDHGIPPAPKRWKGSCKGSAARCNNKIVGARSFIGGDSEDSLGHGTHTSSTAAGNFVSNASSNGLGAGTAARIAPGAHISMYKVCNEKSYKESAIVAGLDMAIKDGVDVLSLSIGPLNASRFDKDIVAIGAFSAVSKGIIVVCAGGNEGPAMSSITNDAPWLLTVAASTVDRSFSADVHLNNTNKISGEALNQVAKLSSKSYPLHYDKMQRNCEYDGLDGLTGKMLVCEATESMPQLYHITDKGVAGVVLINNVTDGYTLMLLDYGPGVVRVMTADGVAVLNHMMSASNPTATFTSKCCQSWRAQASLAPGLNILAAWPPRTRDASPVFHITSGTSMATPHVSGVAALIKGIHPDWSPAAIKSAILTTSDALDNILGPIVDEQHGKASAYATGASHVNPARAADPGLVYDLGITDYAGYICALLGDNALSVIVRNSSMACKNLPKVSESELNYPTITVTLTSTPFTVHRTVTNVGPAKSTYTAKVEATRSLTVRVSPETLVFSKPGEKKTFTVSGSLSWVSGKHVVRSRIVAVAKLGKSPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GWP4</accession>
    <name>A0A0Q3GWP4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">At1g61320/AtMIF1 LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g22781v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GWP4"/>
    <dbReference type="EnsemblPlants" id="KQK15439">
        <property type="protein sequence ID" value="KQK15439"/>
        <property type="gene ID" value="BRADI_1g22781v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15439">
        <property type="protein sequence ID" value="KQK15439"/>
        <property type="gene ID" value="BRADI_1g22781v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GWP4"/>
    <dbReference type="OrthoDB" id="594804at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF76">
        <property type="entry name" value="FBD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="At1g61320/AtMIF1 LRR" evidence="1">
        <location>
            <begin position="71"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK15439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="411" mass="46082" checksum="D3B232897A63E6B4" modified="2016-01-20" version="1">MSVLSSTWSRLWRSCRRNLIFTRKTMVCRSRQIRKRRYLIADLRSDFVENVNGVLRHLGSAAPRDKFVLRFDLCQEQAHAHADTWVNFCAASRAKHVVFDFRPGPWVLAELCSLPLHKLSDAGGSPVVRSLHIMSASLETPPAPGFGGFTNLKELLLDKVVLGDVGWLLASCHAHESLSVMRCSTLQSLSTPRRLRRLWYLSVHLCKEMQKVAVQAPNLSTFKFSTSNDPVSIVLGGSLKMEDVTLVLRSMKDRVDYVFADLPSGVPHARKLSVDFMIDTSVRELARFPTSHFTNLKHLILSLIIFALIGDDTAGLLRLASVLELAPELPPPHRPHIHLKKVHMTGVYGYSNLLKLVLHFARNAVALERMVVDPVSRHATLRVSRGTILTRDVLKESGMKGMSVVYSALEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H6W1</accession>
    <name>A0A0Q3H6W1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100843421</name>
        <name evidence="9" type="ORF">BRADI_1g44070v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560842.1">
        <property type="nucleotide sequence ID" value="XM_003560794.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H6W1"/>
    <dbReference type="FunCoup" id="A0A0Q3H6W1">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK18687">
        <property type="protein sequence ID" value="KQK18687"/>
        <property type="gene ID" value="BRADI_1g44070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843421"/>
    <dbReference type="Gramene" id="KQK18687">
        <property type="protein sequence ID" value="KQK18687"/>
        <property type="gene ID" value="BRADI_1g44070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843421"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H6W1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000069">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000300">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994:SF5">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK18687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="251" mass="28218" checksum="6F2C42E78B5DA80A" modified="2016-01-20" version="1">MGRQPCCDKVGLKKGPWTAEEDQKLVRFLLTHDHYCWRVIPKLAGLLRCGKSCRLRWINYLRPDIKRGLLSDEEEKLVIDLHMQLGNRWSKIAAQLPGRTDNEIKNHWNTHIKKKLRRMGIDPVTHLPMQEIHPLEQPRQQQPSSQLDDAELSLRLDNEDEKAIVPMIQPPQEITAVPPPKGNCSTTFGMDVADCPEPMCLSGIGMDGITEAGWDSFAYGGLGVDPFDQYYPGGGFDPTGSDAILGTLHID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I3P8</accession>
    <name>A0A0Q3I3P8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100827069</name>
        <name evidence="11" type="ORF">BRADI_3g49810v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569954.1">
        <property type="nucleotide sequence ID" value="XM_003569906.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I3P8"/>
    <dbReference type="STRING" id="15368.A0A0Q3I3P8"/>
    <dbReference type="EnsemblPlants" id="KQK00491">
        <property type="protein sequence ID" value="KQK00491"/>
        <property type="gene ID" value="BRADI_3g49810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827069"/>
    <dbReference type="Gramene" id="KQK00491">
        <property type="protein sequence ID" value="KQK00491"/>
        <property type="gene ID" value="BRADI_3g49810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827069"/>
    <dbReference type="OrthoDB" id="771648at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3I3P8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051758">
        <property type="entry name" value="ERF/AP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657:SF19">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="217"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="282"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="431"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="438"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="453"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK00491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="470" mass="50937" checksum="6AD91B6A86C6D67B" modified="2016-01-20" version="1">MEAADRGAGGRGRERRWKGKGASSAAEKQRPLPPQQLAPVLEDAPPAALLRPLKKPDCRLHRSVSSLSSAPASSGSSSVSDPISPPAVGSSLPYPSPGSTRHIFPFAYDPSPAAAPRLLQLLQYSSSLYQQQPLLPQQLQQQQQQTPLQNQQMISFGDAQHEAQQPPLIPPQLMAPEALRYWSEALNLSPRGVLGGLVPVPQSLFQHLLRAPVPAKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEDAAMAYDREAFKLRGENARLNFPDLFLGKGRAGGSGRTSASAAASRSSSSCSSAPPTPDETRTQQARQALLLREQQQQQQQEHLEEPSDIEPTPLFSAAEEQEGIPEAEQSPQLLHKAEQQCGEGSTAMAQAPVTSGGVWGPADEAWFSAWGPGSSVWDYDMDSAHGLFLQSRFAGEQTGMDYVPSAPEAHMAPAPGAASPSLPPRPPPSHSPTFVWKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IR32</accession>
    <name>A0A0Q3IR32_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g20741v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IR32"/>
    <dbReference type="STRING" id="15368.A0A0Q3IR32"/>
    <dbReference type="EnsemblPlants" id="KQJ88728">
        <property type="protein sequence ID" value="KQJ88728"/>
        <property type="gene ID" value="BRADI_4g20741v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88728">
        <property type="protein sequence ID" value="KQJ88728"/>
        <property type="gene ID" value="BRADI_4g20741v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IR32"/>
    <dbReference type="OrthoDB" id="678806at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045282">
        <property type="entry name" value="At4g08330-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33674">
        <property type="entry name" value="METHIONINE-S-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33674:SF3">
        <property type="entry name" value="YIPPEE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24046">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ88728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="74" mass="8202" checksum="AF52E86CDE1EFFBD" modified="2016-01-20" version="1" fragment="single">RFTQVDEISCFPFLTWRSYRPKTKLLCRKCGSSIGYGYGEPAVLCSFDPASSSSGTSQKYLIKIQALQPSDGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IRY6</accession>
    <name>A0A0Q3IRY6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g05850v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK03148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IRY6"/>
    <dbReference type="STRING" id="15368.A0A0Q3IRY6"/>
    <dbReference type="EnsemblPlants" id="KQK03148">
        <property type="protein sequence ID" value="KQK03148"/>
        <property type="gene ID" value="BRADI_2g05850v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03148">
        <property type="protein sequence ID" value="KQK03148"/>
        <property type="gene ID" value="BRADI_2g05850v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IRY6"/>
    <dbReference type="OrthoDB" id="1937734at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IRY6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018467">
        <property type="entry name" value="CCT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040390">
        <property type="entry name" value="TIFY/JAZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077:SF140">
        <property type="entry name" value="PROTEIN TIFY 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09425">
        <property type="entry name" value="Jas_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="200" mass="21042" checksum="854F85F224192B96" modified="2016-01-20" version="1">MAAASTRPGEKPSSFAVACSLLSLYVRQNGGAAAELSLGFSKGDADAQRTKSSLLGAEGEEPGMKKETMELFPQSAGLGSVQDASTPDAVARRQLTVNWSEQPVPAPVTDNKKVETVVPAPASSLPGAQTDAHKPAHPNAADLPIARKASLHRFLEKRNDRLHAKAPYASSPSDEPPVKKEPESQPWLGLGQNAAAKPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ISW8</accession>
    <name>A0A0Q3ISW8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100821485</name>
        <name evidence="2" type="ORF">BRADI_2g08293v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565555.1">
        <property type="nucleotide sequence ID" value="XM_003565507.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ISW8"/>
    <dbReference type="STRING" id="15368.A0A0Q3ISW8"/>
    <dbReference type="EnsemblPlants" id="KQK03508">
        <property type="protein sequence ID" value="KQK03508"/>
        <property type="gene ID" value="BRADI_2g08293v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821485"/>
    <dbReference type="Gramene" id="KQK03508">
        <property type="protein sequence ID" value="KQK03508"/>
        <property type="gene ID" value="BRADI_2g08293v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821485"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055283">
        <property type="entry name" value="TAXIMIN_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33834">
        <property type="entry name" value="SIGNALING PEPTIDE TAXIMIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33834:SF4">
        <property type="entry name" value="SIGNALING PEPTIDE TAXIMIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="80" mass="8524" checksum="FC0FC0298770C7C6" modified="2016-01-20" version="1">MGSDDDDDWRCRPLGFLIGLPFAVLSLALSLAGALVWLLGSAVSCVCPCCVCCAAAANLAVELVQMPVKVVRWFIRLIPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IWN5</accession>
    <name>A0A0Q3IWN5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_4g31785v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IWN5"/>
    <dbReference type="FunCoup" id="A0A0Q3IWN5">
        <property type="interactions" value="2436"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3IWN5"/>
    <dbReference type="EnsemblPlants" id="KQJ90472">
        <property type="protein sequence ID" value="KQJ90472"/>
        <property type="gene ID" value="BRADI_4g31785v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ90472">
        <property type="protein sequence ID" value="KQJ90472"/>
        <property type="gene ID" value="BRADI_4g31785v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IWN5"/>
    <dbReference type="OrthoDB" id="762064at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ90472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="170" mass="18816" checksum="875468643FB4DAAE" modified="2016-01-20" version="1" fragment="single">IPNDATRRATFKNRHDVLLKKASELAILCNIKSCVIVYGKGEAQPEVRSSVDEVVPILHRYKAMPEIGRCKKTVTQEDFLRQRMDKLKKQVHKAVQENRERGTAFLVHKAMLGHLPRLEGLTVEEVTNVGWMVQMKLKSLGDRIARSAGGTLATCSASSLCDVCATGTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JAP7</accession>
    <name>A0A0Q3JAP7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100826666</name>
        <name evidence="4" type="ORF">BRADI_3g16740v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234393.1">
        <property type="nucleotide sequence ID" value="XM_010236091.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JAP7"/>
    <dbReference type="FunCoup" id="A0A0Q3JAP7">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ95360">
        <property type="protein sequence ID" value="KQJ95360"/>
        <property type="gene ID" value="BRADI_3g16740v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66764">
        <property type="protein sequence ID" value="PNT66764"/>
        <property type="gene ID" value="BRADI_3g16740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826666"/>
    <dbReference type="Gramene" id="KQJ95360">
        <property type="protein sequence ID" value="KQJ95360"/>
        <property type="gene ID" value="BRADI_3g16740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66764">
        <property type="protein sequence ID" value="PNT66764"/>
        <property type="gene ID" value="BRADI_3g16740v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JAP7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001029">
        <property type="entry name" value="Os02g0194400 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000887">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001">
        <property type="entry name" value="OS01G0253100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001:SF538">
        <property type="entry name" value="OS02G0227500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="60"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ95360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="351" mass="39082" checksum="9214380675580709" modified="2016-01-20" version="1">MSCMGMLREVVAISSVELQVLTMNLGRNSSICGCFGLNDGSDCHPRIYNEQDILNSLGDGNVMSSINGGPWRSVMYRVHLQDPSRVVVVKKLENKTASAVDASLDDRCQSEVNLLDSICHDNIISLVACILKDNFIVLVYDHNENGSLNQWLHYPELAAEGVLDWPTRLAIAIGVARGIYYLHHGRNNPIVHHNINSSSILLDRDLKPKISGFDFARVNLAEPDQPVPIWELTAGNMFGYTAPEYMTAVTSKVDVYSLGVVMLELVTGRVANEAIADGHLATWAGKHCNRLMKNVVDFSHVIDMAISVPDRVRYFKEMLAMFRLGVACTDKDPQERPPMHEVLSRLRNRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K006</accession>
    <name>A0A0Q3K006_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAM91 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100831030</name>
        <name evidence="5" type="ORF">BRADI_2g11560v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM91 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567584.1">
        <property type="nucleotide sequence ID" value="XM_003567536.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K006"/>
    <dbReference type="FunCoup" id="A0A0Q3K006">
        <property type="interactions" value="1708"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3K006"/>
    <dbReference type="EnsemblPlants" id="KQK04081">
        <property type="protein sequence ID" value="KQK04081"/>
        <property type="gene ID" value="BRADI_2g11560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831030"/>
    <dbReference type="Gramene" id="KQK04081">
        <property type="protein sequence ID" value="KQK04081"/>
        <property type="gene ID" value="BRADI_2g11560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831030"/>
    <dbReference type="OrthoDB" id="275996at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K006">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039199">
        <property type="entry name" value="FAM91"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028097">
        <property type="entry name" value="FAM91_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028091">
        <property type="entry name" value="FAM91_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441">
        <property type="entry name" value="PROTEIN FAM91A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441:SF2">
        <property type="entry name" value="PROTEIN FAM91A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14648">
        <property type="entry name" value="FAM91_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14647">
        <property type="entry name" value="FAM91_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM91 N-terminal" evidence="3">
        <location>
            <begin position="14"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="361"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="532"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="758"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="322"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="478"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="899"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="917"/>
            <end position="930"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK04081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="976" mass="108571" checksum="E0A511A75A8945EF" modified="2016-01-20" version="1">MSAAVEEQMVVKAIREECPWESLPKRLQSTLHTKDEWHRRIVDYCIRKRLQWNTCFARRVYREGEYYEEMMRYLRKNLALYPYHLADYICRVLRISPFRYYCDILFETMKNEQPYDSIPNFTAADALRLTGVGRNEFIDIMNKCRSKKLMWKLNKSIAKDLLPTQPVDFPIEPWWGVCLVNFTLEEFKKLSEEETATIDKICKEEANSYVLFDPKIIDDLYKRGLVYFDVPVYTDDRFKVSRLEGFVSNKDQSYEDPIEELLYAVFVVSSANATVAELAATLQADLYQLQAAASFACRLGWAVKLVDADSLLKDEGGPAFPSSILSDDEEGSNASINSEKSGQQSLSIDTNGPRKISGTAHVGFVVDANVTSYLMMGSLSPGLKSHAITLYEAGKLGDSCIAELCSDLASLEGKKFEGVLEEFANHAFSLRWSLECLKSGGVSTNKNSDNTDDANTSTSSLHDNVTAHLVNVNIEDTTEDGHDEVPHDNHSTSDLDNKDGNVSSMSVDLSEDGESLVRVKAENGSTDTTVLTIQRKYTVDVLRCESLASLAPATLERLFLRDYDIIVSIVPLPSSSVLPGPSGPIHFGPPSYSSMTPWMKLVLYTAGHCGPLSAVFMKGQRFRLLPEPLAGCEKALLWSWDDSVVGGLGGNFEGNLVKGNLLLHCLNSMLKQSAVLVQPLSTNDLNESGNLVTVDIPLPLKNDDNSIASVVAQANLPKEQVFNLASVLKDLSSKFQLSTLGYLRLLRLNRLVESDKFHPENTSYQWVPLSLEFGIPLFNPNLCERICEQVVASHILQKADLAEHNDVMHNVRRRLRELCSEYQATGPVAKLFNKRGSSRDLPRTLINSISSRWNLTDDSASPTNREAPSEHERLKLAGRQRCRTEVVGFDGSTVRSYALSPEHDESTSKPISGDQSSTHEGKSDAEDADSKDVVLPGVNLVFDGAELHPFDIGACLQARQPLWLIAEACVASSTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K932</accession>
    <name>A0A0Q3K932_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g57791v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K932"/>
    <dbReference type="EnsemblPlants" id="KQK20964">
        <property type="protein sequence ID" value="KQK20964"/>
        <property type="gene ID" value="BRADI_1g57791v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20964">
        <property type="protein sequence ID" value="KQK20964"/>
        <property type="gene ID" value="BRADI_1g57791v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K932"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999723" description="Secreted protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK20964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8385" checksum="88BF0E3435A35658" modified="2016-01-20" version="1">MCLPSVWLFGSICRVTCWTPFCKQNPASLYFLPLFKPFSSVSALSRSPEPREAVHSLDHLLPRRRASHGCSAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KL20</accession>
    <name>A0A0Q3KL20_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">BRADI_2g62670v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KL20"/>
    <dbReference type="EnsemblPlants" id="KQK11833">
        <property type="protein sequence ID" value="KQK11833"/>
        <property type="gene ID" value="BRADI_2g62670v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11833">
        <property type="protein sequence ID" value="KQK11833"/>
        <property type="gene ID" value="BRADI_2g62670v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3KL20"/>
    <dbReference type="OrthoDB" id="1111193at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KL20">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000394">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000146">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase IOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF19">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 22" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033238792" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="30"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="546"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="620"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="899"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="902"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="915"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="929"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="648"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQK11833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="958" mass="104845" checksum="8E1B712E8BC35813" modified="2016-01-20" version="1">MAAPPTAARLGLLILVLLLLGFFPAVSLAQMPGFLSIDCGGAANYTDSLGLQWTSDAGLIDSGTPFSIPQSSSSSSPQSSSAQYSTLRYFPADGNKYCYTLKVTTRTRYLLRASFLYADFLLNSSSSDESVFPEFDLYLGATRWSTIVIYDDTRILTRESIVLAASDSLSVCLSNATTGQPFISALELRPLNGSLYRTADESTSFLALAARINFGAPSAAPVRFPDDPYDRIWESDLVRRANYLVDAAPGTTNVSTANPIAVATNERPPEKVMQTAVVGSLGELTYRINLNGFPGNGWAFSYFAEIEEFVAPETRKFKLYIPGLPEVSKPTVDVAENAPGKYRLYQPGFFNVSLPFVLSFAFRKTNDSDRGPILNAFEIYKYVPIDPGSPDAPIMHALASSFAGGHVQGGDPCLPSPWSWVQCTASQPQPRVVSIDLSGKNLTGSIPPELAALPCLAQIRLDNNMLTGPIPDLSAASNLSIIHLENNQLTGRVPSYLSTLPKLTELYLQNNKLSGDIPGALISRGIILNYSGNMHLQAGKQEKRHLIIILSALLGVSLLFAVSICCCVLTRKNIKKNSPEDNLTKPLPAQKLQKSSAPSCEISTETAHPFRLCDLEEATKNFANRIGSGGFGIVYYGKLPDGREIAVKVPTNDSYQGKKQFTNEVSLLSRIHHRNLVAFLGYCHEDGKNILVYEFMMNGTLKEHLHGRDKHITWIQRLEIAEDSAKGIEYLHSGCTPSIIHRDVKTSNILLDKQMRAKVSDFGLSKLVMEESHASTNVRGTLGYLDPQYYISQQLTEKSDIYSFGIILLELISGRPPISTMTFGEHFRNIGPWAKFYYESGDIEAIVDPSISGAGSGYRDVHSIWKIAETAARCIDAEARRRPSMTEVVKEIQEAIALERPPPAREAEGGRRRAASFPASASPGSGAARSHDMVMDSLMRGDDDSSFSDTLLRHPELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KQC9</accession>
    <name>A0A0Q3KQC9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst subunit Exo70 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100836111</name>
        <name evidence="6" type="ORF">BRADI_5g06870v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EXO70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581122.1">
        <property type="nucleotide sequence ID" value="XM_003581074.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KQC9"/>
    <dbReference type="STRING" id="15368.A0A0Q3KQC9"/>
    <dbReference type="EnsemblPlants" id="KQJ82157">
        <property type="protein sequence ID" value="KQJ82157"/>
        <property type="gene ID" value="BRADI_5g06870v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836111"/>
    <dbReference type="Gramene" id="KQJ82157">
        <property type="protein sequence ID" value="KQJ82157"/>
        <property type="gene ID" value="BRADI_5g06870v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836111"/>
    <dbReference type="OrthoDB" id="1922221at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KQC9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF17">
        <property type="entry name" value="EXOCYST SUBUNIT EXO70 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20669">
        <property type="entry name" value="Exo70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="5">
        <location>
            <begin position="233"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ82157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="633" mass="67730" checksum="7446CA87AAF8BCFF" modified="2016-01-20" version="1">MPRAGTRTSSLAMLARPRPRRTLPATVVDDTVASAAALLDKWHPDDDSIYSLFLDSTAEEADEFLRAAASLHRSMLFYASGAETTGTKSLHGGGQGLIQAQALLDTAMRRLQLELHLLLDSLPAVLSFHRRDVEDHDYDDDDDDDYNHDDHEDGNISGSGIGLRETCAHLRAVAEAMMAAGYGKEFVSTFKSRRRASVSGTLQRLLGFSPSLQQAQIPKLAWDQVDAKIIQPWLSGARAAFASVFTAERDLCDGVFSGDNGAAFGDAVFAAIADDQATSVLVVAEAAVARARRAPERLFRVLDVHDALAETILPAVVSAFGEKSEVTSRAVSLVMIKVGDAARGIVASFEAAIQKEPSKATVAAGGAVHPLTRYVINYLAFLADYETALTRIFSSNQQEQFPFGSDTSSFSVGGGGGSTSSSSSSSLDLPSSSTLSLASNPIGWLVFILLRKLDAKAGSYKEAALSYLFLANNTHYVAKKAGPGTRLEGVLGEEWAEAQRAKARGYVDVYVRAAWGSKVIRGGEEAVMEAVAMQDRWVAADEEMGNALRAAVRAAVVPAYRLVYRRQGAAARLTPGDVNAMIGGLFGGHAHAHAVASDESVAADRRRTSQQQEVLIRKQKLKNKQQAAGAGHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LY78</accession>
    <name>A0A0Q3LY78_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucosidebeta-D-glucosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_3g29512v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
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        <text evidence="1">Belongs to the glycosyl hydrolase 1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KQJ97221"/>
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            <end position="54"/>
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        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ97221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97221"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="6">HIG1 domain-containing protein</fullName>
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        <name evidence="7" type="ORF">BRADI_3g47500v3</name>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_3g47500v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00134">
        <property type="protein sequence ID" value="KQK00134"/>
        <property type="gene ID" value="BRADI_3g47500v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="99" mass="11182" checksum="80C80B09C364F29A" modified="2016-01-20" version="1">MAETPSKVESMRKWVVDHKLRAVGCLWLAGITSSVAYNWSRPTMKTSVKLIHARLHAQALTLAALVGSGLVEYYDREYGSSGPKVDRYTRQYMSHSHKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3M7S4</accession>
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        <recommendedName>
            <fullName evidence="2">GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">LOC100824599</name>
        <name evidence="3" type="ORF">BRADI_3g49700v3</name>
    </gene>
    <organism evidence="3">
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblPlants" id="KQK00478">
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        <property type="gene ID" value="BRADI_3g49700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824599"/>
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        <property type="gene ID" value="BRADI_3g49700v3"/>
    </dbReference>
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            <end position="196"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00009414"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="240" mass="26456" checksum="2BEAF55B426BCBBD" modified="2016-01-20" version="1">MPVSSRELRRSMRKSSGHVIGVPVTSKAYALEEAAKGAGACNKDGTDRLAVSLTHPSPYSSFGYKHSSKGQVIHWVSKLGRRAQGFRDHVTLGPKLSETVKGKLSLGARILQAGGVERVFRQAFSAEKGERLVKAHQCYLYTTGGPIAGMLFVSTRKIAFRSDRSLTVTSPAGDVARVPYKVVVPLRRIKRVKPSESAEDPGQKYIHVATVDGFEFWFMGFVSYQRCCKYMQQVISDSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NBR8</accession>
    <name>A0A0Q3NBR8_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC104581999</name>
        <name evidence="2" type="ORF">BRADI_1g15252v3</name>
    </gene>
    <organism evidence="2">
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14294.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312861.1">
        <property type="nucleotide sequence ID" value="XM_024457093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NBR8"/>
    <dbReference type="EnsemblPlants" id="KQK14294">
        <property type="protein sequence ID" value="KQK14294"/>
        <property type="gene ID" value="BRADI_1g15252v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581999"/>
    <dbReference type="Gramene" id="KQK14294">
        <property type="protein sequence ID" value="KQK14294"/>
        <property type="gene ID" value="BRADI_1g15252v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="657247at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33207">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14294.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="244" mass="26745" checksum="C6F3027DBE40100A" modified="2018-03-28" version="2">MSRIQQRKKSHIRQRKNLPNDRLTPMASNGRTKDSMALVRKETSSRPTKRHGVPDKLLELILLLCLSSPLCLDGGHGLVFVPSSPPKVAVNGCHFSLDFLPAGGAGAWEIVDSRHSLLLVAKRKPGWRRHRLLPDLLVCEPVTRRYKLIPSMEDQGLKYHRCIGVFLHDCGAAEDLRHGAAGSSIPCQGSRWFACSTTSTPEYLVTLAPPGPSCSNGPGGERLAGTRRSKRRTGPKSTFKVSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3P1J1</accession>
    <name>A0A0Q3P1J1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100829673</name>
        <name evidence="10" type="ORF">BRADI_5g09580v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311794.1">
        <property type="nucleotide sequence ID" value="XM_024456026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3P1J1"/>
    <dbReference type="FunCoup" id="A0A0Q3P1J1">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ82545">
        <property type="protein sequence ID" value="KQJ82545"/>
        <property type="gene ID" value="BRADI_5g09580v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61045">
        <property type="protein sequence ID" value="PNT61045"/>
        <property type="gene ID" value="BRADI_5g09580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829673"/>
    <dbReference type="Gramene" id="KQJ82545">
        <property type="protein sequence ID" value="KQJ82545"/>
        <property type="gene ID" value="BRADI_5g09580v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61045">
        <property type="protein sequence ID" value="PNT61045"/>
        <property type="gene ID" value="BRADI_5g09580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829673"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3P1J1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF42">
        <property type="entry name" value="COPPER TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0186">Copper</keyword>
    <keyword evidence="3 8" id="KW-0187">Copper transport</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ82545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="167" mass="17115" checksum="C9481892A7F16B42" modified="2016-01-20" version="1">MRGMEGMAMAPPRAPATQHKMATMMHMSFFWGDRAVVLFPGWPGDRGAGAYVLCLLFVLALAALTEALALLSRRLARRGGVGEPTASASAALLLAAVHGGRMGLAYLVMLAVMSFNVGVLLAAVAGHGLGFLLARSKVRTGAARGNGNGNGASPELCGVPQSNGSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R8Q8</accession>
    <name>A0A0Q3R8Q8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_2g48725v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R8Q8"/>
    <dbReference type="EnsemblPlants" id="KQK09554">
        <property type="protein sequence ID" value="KQK09554"/>
        <property type="gene ID" value="BRADI_2g48725v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09554">
        <property type="protein sequence ID" value="KQK09554"/>
        <property type="gene ID" value="BRADI_2g48725v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3R8Q8"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051240">
        <property type="entry name" value="Mito_RNA-Proc/Resp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933:SF11">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="42"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK09554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15544" checksum="09E46200D38348EB" modified="2016-01-20" version="1">MARCLPNRTTYIVLMDGLCKRGMAVDALKMFDEMLERRIVPNVKVYTMIVLSLCNAGRIEDAGRLLCSMKENVWPPDEVTYFQCRYISPNRKGMLRSPNVIRTGGMKRSTVIHLELTRDNWRADQAPATLAPTLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RWZ6</accession>
    <name>A0A0Q3RWZ6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g33535v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_1g33535v3"/>
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        <property type="gene ID" value="BRADI_1g33535v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK17298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblPlants" id="PNT60976">
        <property type="protein sequence ID" value="PNT60976"/>
        <property type="gene ID" value="BRADI_5g08767v3"/>
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    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CG21"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        </location>
    </feature>
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            <end position="401"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="534" mass="56117" checksum="8ECFD7AAA6CCAB0B" modified="2018-03-28" version="1">MSSLMMDQHEEKNRPVKPSGPRHLSGGRFRTAIHTSSSEKSLSGPKGGAEGRPKNWGSKAKGRSSTEPNKAPKKSSATEEIPSCLDARSATSATASQRRQEHAYWPPERCAAAGQQPEDAGAARPANAGEAPEAPRQQHACTQNGNEPASRWEGEQPGGPTSAIIGSKEAVAEGSGDESKQSAAKSCSTGTGAGEPTNPSPPPRKSPREVLGPQDQRHHLQPPDQTHGRHHQPPHACKQNWRWGMHSSSISYGGGGSGMPSRNSLAIFSGSSDHTITERPSAPRRPPRLSTHRGSFPAKNGGGRRHPKPPNTPEKSARSPSNQQASSDHPSRALPGPKPAPPQPHRFFKVRARLRASHQAAKQQQGTASQEPPPRPRSTEQGPPAPAPSEPPEEPLLPAPPVEAKQGTLAEQGAGAAWPAGCGIADSPSPHDPVPSTTCSTSGWGRGTRRDAMGNRGAEGEKEGEREGESGRRGGRRRRSGGRRGEGEGRAQGEVAAGAGVADGGGRQSPTESPTYSPLHKLKSSPNSILNCCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGY3</accession>
    <name>A0A2K2CGY3_BRADI</name>
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        <recommendedName>
            <fullName evidence="8">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">BRADI_5g13090v3</name>
    </gene>
    <organism evidence="9">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_014895555.2"/>
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    <dbReference type="FunCoup" id="A0A2K2CGY3">
        <property type="interactions" value="910"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CGY3"/>
    <dbReference type="EnsemblPlants" id="PNT61277">
        <property type="protein sequence ID" value="PNT61277"/>
        <property type="gene ID" value="BRADI_5g13090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836604"/>
    <dbReference type="Gramene" id="PNT61277">
        <property type="protein sequence ID" value="PNT61277"/>
        <property type="gene ID" value="BRADI_5g13090v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1923003at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CGY3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:response to abscisic acid"/>
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        <property type="entry name" value="WRKY_plant"/>
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    <dbReference type="PANTHER" id="PTHR31221:SF298">
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    </dbReference>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="752" mass="80550" checksum="E59A743F4B8D95A4" modified="2018-03-28" version="1">MVFVLKAFVEQLHAEMDGGHTHIAMAMEQWKQHPNPGPECLMPSFLTDQFPPDPLAEDGSGNEAGFEKHGLSVAVGSPQDEGKPAQLTPHFGGRMSGSSSSLSERMQARNGFSVPKLSMPFATPAGDDSEQSGVQPPIFTVPPGLSPASLLESPVFLSNAMGQASPTTGKLFMLGDADDNNPTRIEAPSIEDGPAAFSFKSLDLKSSRYIAEEMKETLPSNQHPSLPSRDVPVKTETNIQATQGANPLGNKVYFNGQELMKSSYHDSNKRNRLAPDTIVGRDSGSPPDHSQPAADSEANPATMATAATATPAEDGYSWRKYGQKQVKHSEYPRSYYKCTHQSCQVKKKVERSHEGHVTEIIYKGTHNHPKPAAQGRRLPGVPQVHPFGDMDIAADNNNNNDNNGNAGGATQQPNAEARPLWHGGGGMGVQDWRGGDGLEATSSPGELCDSSASMQVHDGTATRFGSPEGVDVTSAVSDEVDGDDRVRAHGSMSQGHNQGAADAGEGDELESKRRKLESCAIEMSTASRAVREPRVVIQTTSEVDILDDGYRWRKYGQKVVKGNPNPRSYYKCTHPGCSVRKHVERASHDLKSVITTYEGKHNHEVPAARNGGGHATSGSAAAQLAHARRPEPPSMAQDGLMMGRLGAPFGLPPRDPLGPMSNFPYSLGVGGYASSGSLPSLPMPAAVEGLKLPMLSPCSLHPLFRHRQAMDQTAAGSGFRVPKGEVKDESNNNSSASAYQQMMNRLPLGHRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CI35</accession>
    <name>A0A2K2CI35_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g19033v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CI35"/>
    <dbReference type="EnsemblPlants" id="PNT61698">
        <property type="protein sequence ID" value="PNT61698"/>
        <property type="gene ID" value="BRADI_5g19033v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61698">
        <property type="protein sequence ID" value="PNT61698"/>
        <property type="gene ID" value="BRADI_5g19033v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CI35"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT61698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="68" mass="8194" checksum="8ABAD9890F01E16C" modified="2018-03-28" version="1">MVGEYARTKCFIHISPRNNMQLVGFLHGIIPYDILTEVPQRACWFKRMCYQIPRIYCFHMPRFVLETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CPB5</accession>
    <name>A0A2K2CPB5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g21939v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CPB5"/>
    <dbReference type="EnsemblPlants" id="PNT63870">
        <property type="protein sequence ID" value="PNT63870"/>
        <property type="gene ID" value="BRADI_4g21939v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63870">
        <property type="protein sequence ID" value="PNT63870"/>
        <property type="gene ID" value="BRADI_4g21939v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CPB5"/>
    <dbReference type="OrthoDB" id="689900at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1135">
        <property type="entry name" value="OS08G0246300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="3">
        <location>
            <begin position="134"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="415"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT63870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="446" mass="49942" checksum="94361FB122FB81CA" modified="2018-03-28" version="1">MHSYFRTCPDFLKPCIFYLLIFHTNRIIRREASDSDGKSAEDNGEGYFSSLLNRNFIQEPSQFITSLNDTRIAWCRVNGFFREYILSQRTEDNLTFELSGRCSLTIQGTARHLIILESWDRDKIVFNSIDFSRLRSITVSGKWKSFFISNSTKLLRVLDLEDAVGVKNDDLHQMVKLLPRLKFLSIRGCHEISHLPSSLGDLRQLQSLDVRHTSIVSLPPSITNLQKLQYIRAGYTYTTTSEEPSPRCLPVSMFSKLCRPGVEVPGGIGKLTALHTLGVVNVSASGGKAILKELKMLTQLRKLGVSGINRKNGDEFCSAISSHGHLESLSVWLDKDSENCFDGISLPLKNVQSLKLYGLVDKLPRGILQYSQDALKEVKLTKLELEMDKLSKYDIAMLGALPKLCILRVKQLKEGTQTEGRPGEPTSPAPKETATEAVVTSLPCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CW92</accession>
    <name>A0A2K2CW92_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g09733v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CW92"/>
    <dbReference type="EnsemblPlants" id="PNT66290">
        <property type="protein sequence ID" value="PNT66290"/>
        <property type="gene ID" value="BRADI_3g09733v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66291">
        <property type="protein sequence ID" value="PNT66291"/>
        <property type="gene ID" value="BRADI_3g09733v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66292">
        <property type="protein sequence ID" value="PNT66292"/>
        <property type="gene ID" value="BRADI_3g09733v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66293">
        <property type="protein sequence ID" value="PNT66293"/>
        <property type="gene ID" value="BRADI_3g09733v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66290">
        <property type="protein sequence ID" value="PNT66290"/>
        <property type="gene ID" value="BRADI_3g09733v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66291">
        <property type="protein sequence ID" value="PNT66291"/>
        <property type="gene ID" value="BRADI_3g09733v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66292">
        <property type="protein sequence ID" value="PNT66292"/>
        <property type="gene ID" value="BRADI_3g09733v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66293">
        <property type="protein sequence ID" value="PNT66293"/>
        <property type="gene ID" value="BRADI_3g09733v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CW92"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="85" mass="9591" checksum="0276B7167CBC4DCA" modified="2018-03-28" version="1" fragment="single">ASTGEHLQIVSDSVQAPDTADGRRPSRTTARFCAAAEDHVVLFYMIMHTHHLMARYIDWICSGRHHFKCSESMLYVSKLLTPSVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CWJ1</accession>
    <name>A0A2K2CWJ1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_3g11028v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CWJ1"/>
    <dbReference type="EnsemblPlants" id="PNT66398">
        <property type="protein sequence ID" value="PNT66398"/>
        <property type="gene ID" value="BRADI_3g11028v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66398">
        <property type="protein sequence ID" value="PNT66398"/>
        <property type="gene ID" value="BRADI_3g11028v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CWJ1"/>
    <dbReference type="OrthoDB" id="670813at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CWJ1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065:SF59">
        <property type="entry name" value="OS02G0661400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065">
        <property type="entry name" value="PLATZ TRANSCRIPTION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT66398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="435" mass="49210" checksum="E01AA8CC66195192" modified="2018-03-28" version="1">MAAARVLSVAAVLPIGGGGPPEAFRRRMRDRVWERWLLSGLFGGGVDLGHAPAPRRATAHGRGDRRRRHHDKYSYPRQEKPLPLLLLALLLCSSSRLSPPPRVPASTAMAGKVCTKCGGSGMEDKMFQCFRCLSWQHKHCRNKDEKDFVGNERLFCDFCLEEDEKNPLKWVKKLASSDFRELCEAYYFCAASTEGFCERCCPVSTCHGHNDHDNIFQVMHPGELLLVRLGDVNKFYDCTDIQPDIPGSVLIHPRPEPLLGGDKSCGYRDCHHEVAEPYTFCSICCKHKENPYSNWMWRVQGLLKRPFIPRHRWNRFCLECHAPFGYQVCEHHGNHFTVAVIESEGGLVVRFPAGIGSEWSNTRWLDCLGAVQSTRKDMDGGVLVLVDFIVDVPLTRPKQLNSERWPKSCSECDNGINEDKEYCSIQCMASKLPGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CX84</accession>
    <name>A0A2K2CX84_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g15115v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CX84"/>
    <dbReference type="EnsemblPlants" id="PNT66644">
        <property type="protein sequence ID" value="PNT66644"/>
        <property type="gene ID" value="BRADI_3g15115v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66644">
        <property type="protein sequence ID" value="PNT66644"/>
        <property type="gene ID" value="BRADI_3g15115v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CX84"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="257" mass="27427" checksum="042ED59300ABC3F1" modified="2018-03-28" version="1">MSPIPRTRGCGLAPPHRLVLARAYSAAILLLLPLRARPPSLSHPPHHALVPNPNSQARIARPPSLGLTALLVPSWTWARHSAQGVQARRSSRAAARRGRGRRVPGSGDTQAAQGARAAWRQGVVGSGCMQMALAPESAGRRQRRAAGMADSAREVAATGRCGGPPLAGNTWLCRPPLPMPMQRCCRAALCLSTAGCQLDAIMPVRCSMMCPNEDNINMHGHPQPRRASLVLVRIITPAVIGISSKCHSYMCLVLNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CXB5</accession>
    <name>A0A2K2CXB5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WPP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100829105</name>
        <name evidence="7" type="ORF">BRADI_3g15400v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CXB5"/>
    <dbReference type="STRING" id="15368.A0A2K2CXB5"/>
    <dbReference type="EnsemblPlants" id="PNT66669">
        <property type="protein sequence ID" value="PNT66669"/>
        <property type="gene ID" value="BRADI_3g15400v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66669">
        <property type="protein sequence ID" value="PNT66669"/>
        <property type="gene ID" value="BRADI_3g15400v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CXB5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.200">
        <property type="entry name" value="WPP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045203">
        <property type="entry name" value="RanGAP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025265">
        <property type="entry name" value="WPP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038214">
        <property type="entry name" value="WPP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46761">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46761:SF2">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13943">
        <property type="entry name" value="WPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WPP" evidence="6">
        <location>
            <begin position="75"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="563"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="569"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="596"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT66669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="605" mass="66222" checksum="817CF97A4E69BCC4" modified="2018-03-28" version="1">MFPHISPSQPKPNSFQIFFVQSPNRRRRRRSLLPGVHAGRPQLPPPPLSARTGFDLGCCAEVPHFLMDSPVWGFSIKLWPLSESTRLMVIDRMTENPSTETLFSRKYGRLDKEEARENAKRIEETCFASVDEHFKEEPDGDGSSAVQLYARETSKLMVEVLKRGPRASAELEAPVVIDAPLEPADTVLDISGGKRAFIDTDEAKELLIPLTKPGNSYKRICFSNRSFGIDAANVAGPILESIKKQITEVDISDFVAGRPEDEALDVMRIFSKALEGSVLRYLNISDNALGEKGVRAFEELLKSQDNLEEFSVMNDGISEEAAKALSELIPSTEILKVLHFHNNMTGDEGALSIAEMVKRSSNLESFRCSATRIGSDGGVALAEALGTCTRLKKLDLRDNLFGVEAGIALSKTLPKLPDLVELYLSDLNLENKGTIAIANALKQSAPRLEVLEMAGNEITAKATQALAECLTVMQSLKKLTLAENELEDRGAVVIAKTLENSHPDLKELDVSTNMLRRAGARCFAQAIADKPGFVQLNINGNFISDEGIEEVKKILKSGKNSLSVLGSLDENDPEGEPGDGDEEGDEEDDEGGLDSKLQNLNVEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYR4</accession>
    <name>A0A2K2CYR4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g22081v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CYR4"/>
    <dbReference type="FunCoup" id="A0A2K2CYR4">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CYR4"/>
    <dbReference type="EnsemblPlants" id="PNT67172">
        <property type="protein sequence ID" value="PNT67172"/>
        <property type="gene ID" value="BRADI_3g22081v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67172">
        <property type="protein sequence ID" value="PNT67172"/>
        <property type="gene ID" value="BRADI_3g22081v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CYR4"/>
    <dbReference type="OrthoDB" id="605154at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170:SF18">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170">
        <property type="entry name" value="BNAC04G53230D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="464"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="496" mass="53824" checksum="ABA1B70B2428385B" modified="2018-03-28" version="1">MADQQDHRSGDSSVIEMTSLAQELTDKLVTLKSSQAQLGDGRVPSGSTRIIVAKVPDSVREADKAEYEHDHVSIGPYNCPRPVPQSHSLHLARREQDKLSSLDDVLTAAMAARNHGGGYLEELARLEPFARSCYYTPVDCYLTSREFVRMLLLDGCYILSCFTAITAPASRNNEPATATENGGVPAPAAGSTAARGGVAMLDDLLVVRDVFFLAENQIPFAVLEKIAELTYVDGNKAAAAVEGIVEYALSLLKAYAVEAPAPAPTAAPGNLLHLLHMHLKPAVMSPAAMEGTSVGRWRTATEYVSAGVTFKKRNVGVNNGDARCILDVKLSGSGSGTLEVPCLDIDGQTWRLLRNLMQLEQQNRETVGTHVTAYCVFMSQVACTDKDVELLSKAKAGVIGHGQGTDAEVAACFADLCKGIMFDRDDSDGNYLKEIWQVLEKRVKSNPRRWMAWLWREHFRNPCLALGLLAAAVALVCGVVQAIYSVLTYRQGFDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D0E0</accession>
    <name>A0A2K2D0E0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g31517v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D0E0"/>
    <dbReference type="EnsemblPlants" id="PNT67746">
        <property type="protein sequence ID" value="PNT67746"/>
        <property type="gene ID" value="BRADI_3g31517v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67746">
        <property type="protein sequence ID" value="PNT67746"/>
        <property type="gene ID" value="BRADI_3g31517v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D0E0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT67746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="65" mass="7463" checksum="64DB393B7A303902" modified="2018-03-28" version="1">MDGSMDGGYIGAVHRTKARLQIVGIWRPLRSWRQTLMKSNPTILGHDVQGRTMDRWMCARTIATY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D217</accession>
    <name>A0A2K2D217_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="4">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC112271988</name>
        <name evidence="14" type="ORF">BRADI_3g38850v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type II topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318102.1">
        <property type="nucleotide sequence ID" value="XM_024462334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D217"/>
    <dbReference type="STRING" id="15368.A0A2K2D217"/>
    <dbReference type="EnsemblPlants" id="PNT68326">
        <property type="protein sequence ID" value="PNT68326"/>
        <property type="gene ID" value="BRADI_3g38850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112271988"/>
    <dbReference type="Gramene" id="PNT68326">
        <property type="protein sequence ID" value="PNT68326"/>
        <property type="gene ID" value="BRADI_3g38850v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10061337at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="13">
        <location>
            <begin position="1"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="334"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="385"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="276"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="PNT68326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="454" mass="52371" checksum="784DD9FEF98E6F43" modified="2018-03-28" version="1">MVLVNGSRGCNSNVPSYNPRDIIANLRRLLNYEHVEPMDPWYRGFKGRIEKISTTVEGSTYSTTGVIKVVDETTLVITELPIHCWTKDYRCFIKSLSKNRKNKTNEFRIKLIKDKCCDDMNLQLKLILEEKNMEKVTEESLEKKLNLITTVGTTNMHLFDRNGINIRKYKTPEEILEEFFGLRLEYYEKRKKAQLDSLDLDLWKITNKVRFILSVGRGDITVLDREAYDLVLELYRKGFSPFPTSSESPEGGKRVLASDYEYLLSIPIGSMAREKLHELYAEKTELEEKIKEVRRSKPRLLWIKDLDALEVELDRFDDLKRAEIERLESLRNRKKPTKKTTANDGEAGVCKPVKSKPAAQKKGKTNTKATVIEIVHDEVPLLEARPANNVVDSSSAHTEGLQVKQELGGEESQRLRKRHQNPQPGPPPARGKGRHQPQQQLMVRTAMARQIRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D5G3</accession>
    <name>A0A2K2D5G3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g56700v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D5G3"/>
    <dbReference type="EnsemblPlants" id="PNT69511">
        <property type="protein sequence ID" value="PNT69511"/>
        <property type="gene ID" value="BRADI_3g56700v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69511">
        <property type="protein sequence ID" value="PNT69511"/>
        <property type="gene ID" value="BRADI_3g56700v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D5G3"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000013">
        <property type="entry name" value="Aspartyl protease AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF762">
        <property type="entry name" value="ASPARTYL PROTEASE AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033311608" description="Peptidase A1 domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="131"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT69511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="463" mass="48617" checksum="F496DC81F21DD1D6" modified="2018-03-28" version="1">MASVAQLLLLPCIIMITYHALVARAGDEKSYKVLSASSLKPGAVCAEPKVRDSSSSGATVPLNHRHGPCSPVPSGKKKQPTFTELLRRDQLRANYIQRQFSDEHYPRTGGLQQSEATVPIALGSLLNTLEYVITVSIGSPAVAHHVHRHRQRRVLVALQSRLYDPGTSSTYAPFSCSAPACAQLGRRGTGCSSGSTCVYSVKYGDGSNTTGTYGSDTLTLAGTSEPLISGFQFGCSAVEHGFEEDNTDGLMGLGGDAQSFVSQTAATYGSAFSYCLPPTWNSSGFLTLGAPSSSTSAAFSTTPMLRSKQAATFYGLLLRGISVGGKTLEIPSSVFSAGSIVDSGTVITRLPPTAYGALSAAFRDGMARYQYQPAAPRGLLDTCFDFTGHGEGNNFTVPSVALVLDGGAVVDLHPNGIVQDGCLAFAATDDDGRTGIIGNVQQRTFEVLYDVGQSVFGFRPGAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D9R5</accession>
    <name>A0A2K2D9R5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g21275v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D9R5"/>
    <dbReference type="EnsemblPlants" id="PNT71007">
        <property type="protein sequence ID" value="PNT71007"/>
        <property type="gene ID" value="BRADI_2g21275v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71007">
        <property type="protein sequence ID" value="PNT71007"/>
        <property type="gene ID" value="BRADI_2g21275v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D9R5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="65" mass="7476" checksum="888AC1E7FBAB3563" modified="2018-03-28" version="1">MHCTYNPRDGSKFWSLCQQQLTGKGRCQKKRALGSLHCCHGVHISGVLFFLRLIMKLLLTLKLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DB07</accession>
    <name>A0A2K2DB07_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g27652v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DB07"/>
    <dbReference type="STRING" id="15368.A0A2K2DB07"/>
    <dbReference type="EnsemblPlants" id="PNT71442">
        <property type="protein sequence ID" value="PNT71442"/>
        <property type="gene ID" value="BRADI_2g27652v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71442">
        <property type="protein sequence ID" value="PNT71442"/>
        <property type="gene ID" value="BRADI_2g27652v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DB07"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21351" checksum="90CD8A5D24F1CE74" modified="2018-03-28" version="1">MVEEDARFTEVWATATIRCEFSSLPVWLAQSPFFLTAETVLMATEPYRCKTLVLRVSIHCEGCKKKVKKALQSVDDRGRKPGESQGSAAGDGAFEYTAKILQVTTEQKGKLKLGKRGSGEKVLGPLPEQEKNKRAKSEGNGESKEGQEPRKLEGNVEATAIHQWRSRETTATDEPSYLVRDISGGRLRDDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DC43</accession>
    <name>A0A2K2DC43_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g36400v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DC43"/>
    <dbReference type="EnsemblPlants" id="PNT71847">
        <property type="protein sequence ID" value="PNT71847"/>
        <property type="gene ID" value="BRADI_2g36400v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71847">
        <property type="protein sequence ID" value="PNT71847"/>
        <property type="gene ID" value="BRADI_2g36400v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DC43"/>
    <dbReference type="OrthoDB" id="1863935at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33127:SF45">
        <property type="entry name" value="OS05G0143700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33127">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="2"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="178" mass="19650" checksum="BCE45E8649B6FAE5" modified="2018-03-28" version="1">MAAVGGKVYFEISAHDLGVIEFDSNPESSSSSSTVAELGGIDVDMVELPSHMPMASTYLVESAGELFLVVVFFDGENFHEVAEVRVYRMDFSKPAWCEVDRIGDDHRIGISNFGASCSAYGGLKGNCVYFLNHLATAENFLHVFDLQDGTQQVQRPFRDMGFTLPPRPPFWLLPAESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DCK8</accession>
    <name>A0A2K2DCK8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_2g38370v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DCK8"/>
    <dbReference type="FunCoup" id="A0A2K2DCK8">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DCK8"/>
    <dbReference type="EnsemblPlants" id="PNT72011">
        <property type="protein sequence ID" value="PNT72011"/>
        <property type="gene ID" value="BRADI_2g38370v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72011">
        <property type="protein sequence ID" value="PNT72011"/>
        <property type="gene ID" value="BRADI_2g38370v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DCK8"/>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000474">
        <property type="entry name" value="Wall-associated receptor kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF468">
        <property type="entry name" value="OS01G0310500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="42"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT72011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="459" mass="51529" checksum="8E7AA0F488DA592E" modified="2018-03-28" version="1">MDYQGSNVREFLQINGRVVLERVDNNCSLQSFTQKDTEDITNGYRSLLGEGGFGKVYKGRLLDNQRPLAVKRYKNGTKKEEFAKELIVHSQINHKNVVRLLGCCTEENSLAIVMEFICNGNLENILHCCNANSHAPFALDRRLDIAIESAEALSYMHSMYSPVLHGDIKPANILLDENLRPKISDFGIARLLSADEAQRTRTVIGCIGYMDPLFCQTGILTPKSDVYSFGVVLLEMLTRKKAAGGDTNLTQSFIEALRKGKKVRQMFDGEIASEKGNIKLLDGIAKLAAECLKMEDKIRPEMVEVADRLRTIRKVLQKRKGRSSIDDRMEITTNFSNDPLMVPTISLAELKQITRNFRNDTLIGGGLYGNVFFGVLKDGQKSAILKLDPNSIEETFLEFPVVSRFKHEMLSSFLDTVLIETIVFLLMSMHPGDPCTIFFMVTRVLGEPNQDLFCHGGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGA3</accession>
    <name>A0A2K2DGA3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF506 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC104583356</name>
        <name evidence="2" type="ORF">BRADI_2g56800v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233566.2">
        <property type="nucleotide sequence ID" value="XM_010235264.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGA3"/>
    <dbReference type="STRING" id="15368.A0A2K2DGA3"/>
    <dbReference type="EnsemblPlants" id="PNT73297">
        <property type="protein sequence ID" value="PNT73297"/>
        <property type="gene ID" value="BRADI_2g56800v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583356"/>
    <dbReference type="Gramene" id="PNT73297">
        <property type="protein sequence ID" value="PNT73297"/>
        <property type="gene ID" value="BRADI_2g56800v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583356"/>
    <dbReference type="OrthoDB" id="691424at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01615">
        <property type="entry name" value="A_thal_3542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF34">
        <property type="entry name" value="T14N5.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Wang M."/>
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    </evidence>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Tice H."/>
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                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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</entry>
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        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="7">cv. Bd21</strain>
        </source>
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        <text evidence="2">Belongs to the NET family.</text>
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    </dbReference>
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        <property type="gene ID" value="BRADI_1g18347v3"/>
    </dbReference>
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    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258">
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            <begin position="1"/>
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        </location>
    </feature>
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        </location>
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        <location>
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            <end position="216"/>
        </location>
    </feature>
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        </location>
    </feature>
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    </feature>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT74590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="375" mass="42009" checksum="AAB3B3BC42F36920" modified="2018-03-28" version="1">MSGFFSRLLLRLSIFSVSLYFGGFKSCPMGLNFVTPACLLPCGLAHVVPEFHVSSSEDIIFLRILFVCLFSVVSEGKEIEEMMQKEQPQAWWFDSHNLARPSPWLGNTLSELDDKTKQMLKLIDQDADSFAQRAEMYYKKRPVLVDMLGDLYRAHRSLAEQCDLLKHGGGTRRSKVFASPSPPCTKSWSPAASMDGKAGAGSSSSSTSLCSGSSYDSESEVDDPEEEHHHHGDHHKVHHHHGDHQKASEGKTEAEKKEQQPEQQQEEAAELMEEIRMLKEQNSELQKAMEESTAMYKAELAAKDEEKREVIRQLAASLDVVQGENSTLRECIKSSKHHHRPAAARAFDLKKLTRDLFSAKLFTAHCRPSGPVVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DLS5</accession>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DLS5"/>
    <dbReference type="EnsemblPlants" id="PNT75223">
        <property type="protein sequence ID" value="PNT75223"/>
        <property type="gene ID" value="BRADI_1g28836v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75223">
        <property type="protein sequence ID" value="PNT75223"/>
        <property type="gene ID" value="BRADI_1g28836v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DLS5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="237" mass="25134" checksum="02C6F6C5E069794A" modified="2018-03-28" version="1">MAPPLPPEFARRRPSPPATSPPPLTPPPPLWLQPPPFPLPRPASRAPRREPPQAEGVAAARARAAAGRPLVALPCHHGRPTALSPCRLLLLHGSHGSGGGVDHGELVRDEHRASRSSSSSPSRVPLLYPPRAVVPTPSSTSCSCAMVMAAVRPTNAAAEAPATSPAPALLRPTSLVPHTRQLHLPQCGRREQVTRASARDTWKKMHGRGRETSKEASHDKGNLFVTSLTNSTSCLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DMJ9</accession>
    <name>A0A2K2DMJ9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g33695v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DMJ9"/>
    <dbReference type="EnsemblPlants" id="PNT75504">
        <property type="protein sequence ID" value="PNT75504"/>
        <property type="gene ID" value="BRADI_1g33695v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75504">
        <property type="protein sequence ID" value="PNT75504"/>
        <property type="gene ID" value="BRADI_1g33695v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DMJ9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT75504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="73" mass="8406" checksum="47ED8BAD7B522C95" modified="2018-03-28" version="1">MAIHVPFGDKQHQPTLYLDNFENNRLAVVALSEQIFDSSFQERPDNRVDNLPIKQICSWMEVADTDKEGEDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQP6</accession>
    <name>A0A2K2DQP6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BRADI_1g50161v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQP6"/>
    <dbReference type="STRING" id="15368.A0A2K2DQP6"/>
    <dbReference type="EnsemblPlants" id="PNT76602">
        <property type="protein sequence ID" value="PNT76602"/>
        <property type="gene ID" value="BRADI_1g50161v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76602">
        <property type="protein sequence ID" value="PNT76602"/>
        <property type="gene ID" value="BRADI_1g50161v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DQP6"/>
    <dbReference type="OrthoDB" id="688481at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DQP6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001161">
        <property type="entry name" value="Os11g0601500 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000028">
        <property type="entry name" value="Putative DUF26-domain receptor-like protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF345">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036319261" description="non-specific serine/threonine protein kinase" evidence="19">
        <location>
            <begin position="20"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="4"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="21">
        <location>
            <begin position="130"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="336"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="PNT76602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="589" mass="65986" checksum="7F2A377E5DD5A873" modified="2018-03-28" version="1">MATIGVLLLLTLLLELVSADVFCDNLKSVVSVLAKNTSSSPEHFATATFGQAPDIVYALALCRGDVLDDSSCANCVTNTFVNSVLNQMPPPPFQCYYGYVYFGLCLIVYSFNDDIFAPFNATGLEPFERWNVRTITSDVRLTTSEAGLVASMIHDLLVDTVDTAARMTPSRFATGLMDSRTSFPMVYSLAQCRPDLSADDCMSCLHHLLDTINSTMSRRIGAQIHVIWCSLRYEVSVFYKGEPMLHHKAPLAPAPAPTRTKRQRHMSKLWAIAIVVVALAAAAFLCFILYHRQLMRLKGLRRAQDLEGEEQSVWQGKNSVFSIFDFEQVLQATNNFSQENKLGQGGFGAVYKGQFPEGLEIAVKRLASHSGQGFNEFRNEVQLIAKLQHRNLVRLLGCCSEEEEKLLVYEYLRNKSLDFFIFDENKRALLDWSKLVTIIEGIAHGLLYLHKHSRLRVIHRDLKPGNILLDAEMNPKIADFGLAKIFSSDNTEGNTTRRVVGTYGYMAPEYASEGVFSIKSDVFSFGVIIFEILSGKRNSGSQQCGDFINLLGYAWQLWVEGRWIDLIDATLVPKSDSTEMMRCCQNAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DTV8</accession>
    <name>A0A2K2DTV8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g67491v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DTV8"/>
    <dbReference type="EnsemblPlants" id="PNT77705">
        <property type="protein sequence ID" value="PNT77705"/>
        <property type="gene ID" value="BRADI_1g67491v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77705">
        <property type="protein sequence ID" value="PNT77705"/>
        <property type="gene ID" value="BRADI_1g67491v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DTV8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT77705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="132" mass="13105" checksum="3D477FB9433AB6D6" modified="2018-03-28" version="1">MPPLATPLDLLAPAVLSQAPGSSLLPLLDPQIYFHKTPPAHPENVTLVLGLPWPPLSPSGPARPTSSSPATTALLPTSSLAACTTTSARTAMASRHGSGAAAVASDSEAGVTPTPSQLAGVGTGGPRCTSYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLB8</accession>
    <name>I1GLB8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100829136</name>
        <name evidence="5" type="ORF">BRADI_1g03070v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559213.2">
        <property type="nucleotide sequence ID" value="XM_003559165.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLB8"/>
    <dbReference type="FunCoup" id="I1GLB8">
        <property type="interactions" value="445"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GLB8"/>
    <dbReference type="EnsemblPlants" id="KQK12341">
        <property type="protein sequence ID" value="KQK12341"/>
        <property type="gene ID" value="BRADI_1g03070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829136"/>
    <dbReference type="Gramene" id="KQK12341">
        <property type="protein sequence ID" value="KQK12341"/>
        <property type="gene ID" value="BRADI_1g03070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829136"/>
    <dbReference type="eggNOG" id="ENOG502R3GX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061892_1_0_1"/>
    <dbReference type="OMA" id="YARKEMD"/>
    <dbReference type="OrthoDB" id="636954at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009827">
        <property type="term" value="P:plant-type cell wall modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15801">
        <property type="entry name" value="PMEI-like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.40:FF:000007">
        <property type="entry name" value="Pectinesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051955">
        <property type="entry name" value="PME_Inhibitor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080:SF69">
        <property type="entry name" value="OS03G0830600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080">
        <property type="entry name" value="PECTINESTERASE INHIBITOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093934" description="Pectinesterase inhibitor domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="4">
        <location>
            <begin position="32"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK12341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="211" mass="22078" checksum="08DAD86E28ABABA0" modified="2012-06-13" version="1">MATRRTTSGTFFFFLLPLILAAAAAATIVKEAATVDINAICSKTSHPDFCTTTLSAIPETKTADARGLAEIAIRATSRVGATAGAYARKELDIVKDNALWQCLDECAEDIEDAVAHLDDSEGQIGDAKYDLVAKYLDAAEKDLWSCDESCRDTPASSVKTTVLAKNTDFEKMMAVTNELIKMTGKAAAAAAADGPSSEPASAPEPAAAAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1GN74</accession>
    <name>I1GN74_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100839954</name>
        <name evidence="9" type="ORF">BRADI_1g08326v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559391.1">
        <property type="nucleotide sequence ID" value="XM_003559343.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GN74"/>
    <dbReference type="FunCoup" id="I1GN74">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13148">
        <property type="protein sequence ID" value="KQK13148"/>
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="EMBL" id="KQK16210.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK16210"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXE4</accession>
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        </recommendedName>
    </protein>
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        <name evidence="13" type="primary">LOC100832935</name>
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    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="6 11"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563638.1">
        <property type="nucleotide sequence ID" value="XM_003563590.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXE4"/>
    <dbReference type="STRING" id="15368.I1GXE4"/>
    <dbReference type="EnsemblPlants" id="KQK17719">
        <property type="protein sequence ID" value="KQK17719"/>
        <property type="gene ID" value="BRADI_1g36260v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832935"/>
    <dbReference type="Gramene" id="KQK17719">
        <property type="protein sequence ID" value="KQK17719"/>
        <property type="gene ID" value="BRADI_1g36260v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832935"/>
    <dbReference type="eggNOG" id="KOG1557">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031243_0_2_1"/>
    <dbReference type="OMA" id="GDAMQKW"/>
    <dbReference type="OrthoDB" id="36455at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:glycolytic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029768">
        <property type="entry name" value="Aldolase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033379">
        <property type="entry name" value="FrucBisAld_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00158">
        <property type="entry name" value="ALDOLASE_CLASS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK17719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="360" mass="37772" checksum="59FC0FCCF1BC73C8" modified="2012-06-13" version="1">MSAFVGKYADELIKTAKYIATPGKGILAADESTGTIGKRLASISVENVESNRQALRELLFTTPGAVEYLSGVILFEETLYQKSSDGTPFVDILKAGNVVPGIKVDKGTVEIAGTDGETTTQGLDSLGARCAKYYEAGARFAKWRAVLKIGAAGEPSELAVKQNAEGLARYALICQENGLVPIVEPEILTDGGHDIKVCAAATERVLAAVYKSLNDHKVLLEGTLLKPNMVTPGSDSPKVGAEVIAEYTVAALRRTVPPAVPGIVFLSGGQSEEEATKNLDAMNKLAVGKPWTLTFSFGRALQQSTLKKWGGKKENVAAAQATFLARCKGNSEATLGKYAGAGAGGDAAASESLHVAGYKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1H1S6</accession>
    <name>I1H1S6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDP-mannose 4,6-dehydratase</fullName>
            <ecNumber evidence="5">4.2.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100833109</name>
        <name evidence="10" type="ORF">BRADI_1g51460v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O</text>
            <dbReference type="Rhea" id="RHEA:23820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57964"/>
            <dbReference type="EC" id="4.2.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.47" evidence="5"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557227.1">
        <property type="nucleotide sequence ID" value="XM_003557179.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H1S6"/>
    <dbReference type="FunCoup" id="I1H1S6">
        <property type="interactions" value="1606"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H1S6"/>
    <dbReference type="EnsemblPlants" id="KQK19944">
        <property type="protein sequence ID" value="KQK19944"/>
        <property type="gene ID" value="BRADI_1g51460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833109"/>
    <dbReference type="Gramene" id="KQK19944">
        <property type="protein sequence ID" value="KQK19944"/>
        <property type="gene ID" value="BRADI_1g51460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833109"/>
    <dbReference type="eggNOG" id="KOG1372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_14_0_1"/>
    <dbReference type="OMA" id="HWQTVNY"/>
    <dbReference type="OrthoDB" id="10253554at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008446">
        <property type="term" value="F:GDP-mannose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042351">
        <property type="term" value="P:'de novo' GDP-L-fucose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05260">
        <property type="entry name" value="GDP_MD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000102">
        <property type="entry name" value="GDP-mannose 4,6-dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00955">
        <property type="entry name" value="GDP_Man_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006368">
        <property type="entry name" value="GDP_Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01472">
        <property type="entry name" value="gmd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715:SF1">
        <property type="entry name" value="GDP-MANNOSE 4,6 DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715">
        <property type="entry name" value="GDP-MANNOSE 4,6-DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="9">
        <location>
            <begin position="37"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK19944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="377" mass="41681" checksum="0198C340E71B92AD" modified="2012-06-13" version="1">MAAPHSNGGAAAGAGEEEAVAVASVPRSLAPPRKVALVTGITGQDGSYLTELLLSKGYEVHGLIRRSSNFNTQRLDHLYHDPHATPSAARPPMRLHYADLSDSSSLRRALDHVLPDEVYNLAAQSHVAVSFEIPDYTADVTATGALRLLEAVRLSAKPMRYYQAGSSEMFGSTPPPQSEDTPFHPRSPYAAAKVAAHWYTVNYREAYGIFACNGVLFNHESPRRGENFVTRKITRAVGRIKVGLQTKVFLGNLSAARDWGFAGDYVEAMWLMLQQDKPGDYVVATEECHTVEEFLQAAFGYAGLDWKDHVVIDKKYFRPAEVDCLKGDSSKSRRELGWKPKVGFQQLVEMMVDNDIELAKKEKVLVDAGYRDPKQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H207</accession>
    <name>I1H207_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein transport protein sec16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100839880</name>
        <name evidence="10" type="ORF">BRADI_1g52140v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SEC16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561234.1">
        <property type="nucleotide sequence ID" value="XM_003561186.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="I1H207">
        <property type="interactions" value="949"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H207"/>
    <dbReference type="EnsemblPlants" id="KQK20046">
        <property type="protein sequence ID" value="KQK20046"/>
        <property type="gene ID" value="BRADI_1g52140v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT76722">
        <property type="protein sequence ID" value="PNT76722"/>
        <property type="gene ID" value="BRADI_1g52140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839880"/>
    <dbReference type="Gramene" id="KQK20046">
        <property type="protein sequence ID" value="KQK20046"/>
        <property type="gene ID" value="BRADI_1g52140v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76722">
        <property type="protein sequence ID" value="PNT76722"/>
        <property type="gene ID" value="BRADI_1g52140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839880"/>
    <dbReference type="eggNOG" id="KOG1913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="FAQQQFI"/>
    <dbReference type="OrthoDB" id="8918678at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H207">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09233">
        <property type="entry name" value="ACE1-Sec16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1030:FF:000005">
        <property type="entry name" value="Protein transport protein sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024340">
        <property type="entry name" value="Sec16_CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402:SF31">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402">
        <property type="entry name" value="RGPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12932">
        <property type="entry name" value="Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec16 central conserved" evidence="9">
        <location>
            <begin position="689"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="Sec16 Sec23-binding" evidence="8">
        <location>
            <begin position="875"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="48"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="449"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1160"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1306"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1413"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="466"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1167"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1196"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1225"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1243"/>
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        </location>
    </feature>
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        <location>
            <begin position="1310"/>
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        </location>
    </feature>
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        <location>
            <begin position="1463"/>
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        </location>
    </feature>
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        <location>
            <begin position="1480"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1498"/>
            <end position="1519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT76722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1554" mass="165785" checksum="C1C94A0E721564DC" modified="2012-06-13" version="1">MASDSDDADFFDKLVDDDDDDQVVPPAAAAQAAYAAVADISSLSLADDAVDEPELPAAATAPADAADPPPALTPPKAGDDVPAVPSDAEVLAALPEVKAAAVAAVPKTEAVATPEGGSPGSGKAVRTTVKQVQWSVFGADSSAEADPFGDLLADGAGGDSFFGVSGAQVQEPRSSVAAAGSVGAAPADHGFFSGVGNNAISQPGWGARAGGFLADGAAGDEAAFFGASVVEDQGVQTGSVVATSGVGAAYHGFFNGVDSSANSSQSYMGASAFESADQNTNVQSEWASGSVNSSEPNYLENRYPGWMFDEATQNWYQVPTTSAQGFADYGGAVTAVGSGNVQQLQQQLSGSYMHNSSQAGLETIAEEGGTTVMTWGQDQSSAVAAEYPPNMLFYAEYPGYYFDTNTQDWYTLESYQQSFAQVATATAASDAFVGHNVGNAMNSQASNFNQQNQWQQSSYSSHQHTETQITQQANTEPSEPSTNYRTGINTFVPSLEPSMNHSTSVSTFMPSTSQYSSGESHQVSHKGFEPTSYQNVQKGFEPYKSNQSTFTNHDSGYKVSEPSTGHQGFKPVANNHKSTGFVPSTGHQIAHREFEPSKDNQAYHVAHEPSVAHGYVNSNGFAEQQTFVPSTTGSMYQTQTHIDPAAHMHLTNNYLISENSMNFAQQQPVSQQFGYSPHQERSSAGRPPHALFAFGFGGKLVVMKETSSMTTSFDSGNQGNSRGMVSIHNISEIVADKIDQSSITNGSALGYFHALCRRPIPGPLVGGSPATKDVSKWLDEIIGSYESSVREFQGGDVQKLLVSLLKISYQHYGKLRSSFGSDPLQEGMDGPDTAVTTLFSSCNSNSAHIREYGAITHCMKNIPSENQIQATAQNVQSLLVSGRRKDALQYAQEGQLWGPALVLALQLGDKFYADTVKKMAYSHFMSGSPLRTLCLLIAGQPADVFHAENPIDANYGTLHRPQEAAEGTPKGMLDDWQQNLAIITANRTKGDDLVITHLGDCLWKEKNEVAAAHSCYLVAELNIDAYSETARMCLLGADHLRCPRTFTSPEAIQRTELYEYAKVLGNSQYILLPFQPYKLVYAYMLAEVGKISDSLRYCQASLKVLKASGRAPELEAWKQLFSSLEERIRTHQQGGYGTNLAPAKLVGKLFTSLDKSLSRMMGTQPTSLPPLAQPMPPLAQGSSTERDLYSAPPATNFVNNNQSATPMSFSASEQSMSDMTGNSGPDRKGTHNRSVSEPDFNRAPQQGTRSDNPQSTSGSGNSRFGWLLQKTMGLVSKSPHQAKLGEQNKFYYDEKLKRWVEQGAEVPAAEEPPLPPPPTKASFQNGVPDYNLTGPSIGGYTANGFAETKPPNPSELMTGMPPMPPSQNQFSARGRAGVRSRYVDTFNKGGAATAASYNKPAMPSMKPLPGAGFFVPTPAADEPKQTTESTADVHSATAYQDGPSSSPAVERSFASPPPPLVPMQSSIQRHPSMDNILTPSGSGNSSFSKSRAASWSGTYSDQLSTTAASRSPDGQTMQSPVLMPPGRQPSHSRSHSNSSIQLNGLGEDLQEVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1H3M2</accession>
    <name>I1H3M2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100836832</name>
        <name evidence="4" type="ORF">BRADI_1g57090v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752109.1">
        <property type="nucleotide sequence ID" value="XM_014896623.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H3M2"/>
    <dbReference type="EnsemblPlants" id="KQK20848">
        <property type="protein sequence ID" value="KQK20848"/>
        <property type="gene ID" value="BRADI_1g57090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836832"/>
    <dbReference type="Gramene" id="KQK20848">
        <property type="protein sequence ID" value="KQK20848"/>
        <property type="gene ID" value="BRADI_1g57090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836832"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013137_15_4_1"/>
    <dbReference type="OMA" id="YFCTRTS"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045899">
        <property type="entry name" value="ATL71-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46719:SF5">
        <property type="entry name" value="OS07G0159600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46719">
        <property type="entry name" value="TRANSCRIPTION FACTOR C2H2 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="107"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK20848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="170" mass="17614" checksum="CCB09B44149ACA6D" modified="2012-06-13" version="1">MSTGGGGSPSPAGHGGMFGSSGAGGFGYGVVVSVGILLLLAIIAFAAFYFCCARTAPTPGAGAPRHGDEEAGGGVDEATLEAYPTMAYGELWKKQRRRGQEETEACCPVCLESYGEAEVVRALPECGHVFHRGCVDPWLRRRATCPVCRTSPLPSPLAEVTPLALARPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1H5V5</accession>
    <name>I1H5V5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxin efflux carrier family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100840886</name>
        <name evidence="7" type="ORF">BRADI_1g63446v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313360.1">
        <property type="nucleotide sequence ID" value="XM_024457592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H5V5"/>
    <dbReference type="STRING" id="15368.I1H5V5"/>
    <dbReference type="EnsemblPlants" id="KQK21843">
        <property type="protein sequence ID" value="KQK21843"/>
        <property type="gene ID" value="BRADI_1g63446v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840886"/>
    <dbReference type="Gramene" id="KQK21843">
        <property type="protein sequence ID" value="KQK21843"/>
        <property type="gene ID" value="BRADI_1g63446v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2722">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044945_2_0_1"/>
    <dbReference type="OMA" id="AICHDEN"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080162">
        <property type="term" value="P:endoplasmic reticulum to cytosol auxin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039305">
        <property type="entry name" value="PILS2/6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31419:SF9">
        <property type="entry name" value="AUXIN EFFLUX CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31419">
        <property type="entry name" value="PROTEIN PIN-LIKES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK21843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="44218" checksum="8682EC78067465D0" modified="2012-06-13" version="1">MEVWSLVSGGGGQPILTSMKFAVLPIAKVFTMCFMGFLMATKHVGILNARGRKLLNGLVFSLLLPCLIFTQLGRAITVQKVLDWWYIPVNIVLGTVSGSLVGLVVAWIVQPPRPYFKFTIVHIAIGNNGNMPLVLVAALCRDPLNPFGDPNKCKQDGNAYVSFGQWVGAIILYSYVFQMLALPPGETYDGSRVKKLSSKAVADGRAPEKVPLLTSAAPEQAVIHASKCSKAMGILKFFVEKFKLKQLLQPPVIASALGILIGTSPSLKHFILANDAPLFFFTDACIILGEAMIPCILLALGGNLVDGPGAGSKKLGLRTTVAIIFGRLVLVPPAGMGVVNLAERLGLFPKGDTMFKFVLLLQHSMPTSVLSGAVANLRGCGEESAAVLFWMYVCATFSVAGWMVLYIRMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1H609</accession>
    <name>I1H609_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lipoyl(octanoyl) transferase</fullName>
            <ecNumber evidence="3">2.3.1.181</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100842681</name>
        <name evidence="10" type="ORF">BRADI_1g63900v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LipB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558023.1">
        <property type="nucleotide sequence ID" value="XM_003557975.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H609"/>
    <dbReference type="STRING" id="15368.I1H609"/>
    <dbReference type="EnsemblPlants" id="KQK21911">
        <property type="protein sequence ID" value="KQK21911"/>
        <property type="gene ID" value="BRADI_1g63900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842681"/>
    <dbReference type="Gramene" id="KQK21911">
        <property type="protein sequence ID" value="KQK21911"/>
        <property type="gene ID" value="BRADI_1g63900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842681"/>
    <dbReference type="eggNOG" id="KOG0325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035168_1_3_1"/>
    <dbReference type="OMA" id="GEVTYHC"/>
    <dbReference type="OrthoDB" id="19908at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd16444">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000063">
        <property type="entry name" value="Octanoyltransferase LIP2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00013">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010925">
        <property type="entry name" value="PRK14345.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993">
        <property type="entry name" value="OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993:SF15">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIP2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016262">
        <property type="entry name" value="LPLase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="9">
        <location>
            <begin position="37"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="82"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="163"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="8">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK21911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="242" mass="26268" checksum="F3C446B21FB06A6C" modified="2012-06-13" version="1">MSGGGAKRVLEAWKLGVVKYGDALRLQERLVADRRAGRIPDLVLSLQHPPTYTLGKRRTDHNLLVPESSLGGLGAELHRTERGGDVTFHGPRQAVLYPILSLRDIGLGARRYVEGLESAMIEVASLYGIKARPGGACETGVWVGDRKIGAIGVRISSGFTSHGLAFNIDPDLGYFKHIVPCGIADKEVTSLRREAAVELPPDEVIHDQLVQSLARTFYFSDVKLKDDSECAEMMCSAATSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6B1</accession>
    <name>I1H6B1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF3511 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100839633</name>
        <name evidence="2" type="ORF">BRADI_1g64730v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558099.1">
        <property type="nucleotide sequence ID" value="XM_003558051.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6B1"/>
    <dbReference type="STRING" id="15368.I1H6B1"/>
    <dbReference type="EnsemblPlants" id="KQK22042">
        <property type="protein sequence ID" value="KQK22042"/>
        <property type="gene ID" value="BRADI_1g64730v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK22043">
        <property type="protein sequence ID" value="KQK22043"/>
        <property type="gene ID" value="BRADI_1g64730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839633"/>
    <dbReference type="Gramene" id="KQK22042">
        <property type="protein sequence ID" value="KQK22042"/>
        <property type="gene ID" value="BRADI_1g64730v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22043">
        <property type="protein sequence ID" value="KQK22043"/>
        <property type="gene ID" value="BRADI_1g64730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839633"/>
    <dbReference type="eggNOG" id="ENOG502S6MA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120180_0_0_1"/>
    <dbReference type="OMA" id="CKECTRY"/>
    <dbReference type="OrthoDB" id="660385at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021899">
        <property type="entry name" value="DUF3511"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33193">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (DUF3511)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33193:SF13">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12023">
        <property type="entry name" value="DUF3511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12678" checksum="DD0F1094AE0BF692" modified="2012-06-13" version="1">MAAADYDRPYRPYAPHAADYDRPYRNEIVPYGDRRIDLVVKPPPSRSPPPPLPVTKSGGGGGLGSAWCFSDPEVKRRRRVASYKAYSVEGKVKASFRRGFRWIKDKCTGLIHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6F0</accession>
    <name>I1H6F0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Laccase</fullName>
            <ecNumber evidence="5 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100820945</name>
        <name evidence="18" type="ORF">BRADI_1g65100v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Secreted</location>
            <location evidence="3 13">Extracellular space</location>
            <location evidence="3 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="5 13"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558123.1">
        <property type="nucleotide sequence ID" value="XM_003558075.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6F0"/>
    <dbReference type="EnsemblPlants" id="KQK22086">
        <property type="protein sequence ID" value="KQK22086"/>
        <property type="gene ID" value="BRADI_1g65100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100820945"/>
    <dbReference type="Gramene" id="KQK22086">
        <property type="protein sequence ID" value="KQK22086"/>
        <property type="gene ID" value="BRADI_1g65100v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100820945"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006504_6_3_1"/>
    <dbReference type="OMA" id="DERCIVL"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000062">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF287">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="11 13" id="KW-0186">Copper</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982648" description="Laccase" evidence="13">
        <location>
            <begin position="30"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="17">
        <location>
            <begin position="38"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="164"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="443"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK22086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="599" mass="65310" checksum="EECAB1042154408D" modified="2012-06-13" version="1">MESASGVMRFCCCVSSLLLLCFLLPCALAEERFYEFVVQETVVKRLCKTQKIITVNGQFPGPTIEVHDGDTLAIRAVNMAQYNVTLHWHGLRQLRNGWADGPEFVTQCPIRPGSSYTYRYTIQGQEGTLWWHAHSSWLRATVHGALIIHPKRGLPYPFPKPNKEFPVLLAEWWRKDPIAVIRQSMVTGAPPNISDTILINGQPGDFLECSSQETSIIPVVAGETNLLRIINAAMNSELFVSLAGHKMTVVAADAVYTRPFETTVVLLGPGQTTDVLVTADAAPARYYLAARVYASAQGVPFDNTTATAIFQYKNAAGCPTTTNPALNGPVGRPSQRSSAHPGRAGPAPLMPALPAFNDTNTATAFSKSIRSPRPVKVPGPVTQEVFTTVGFGLFNCRPGPFCQGPNNTRFAASMNNVSFQLPNTVSLLQAHYHHVPGVFTEDFPAVPPVIFDFTSQNVPRSLWQPVKGTRLYRVKYGAVVQMVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYDARRDAAKFNMVDPPSRNTIGVPVGGWAVVRFVADNPGVWLVHCHIDAHLTGGLGMALLVEDGKAELQATVPPPLDLPICGVDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1H7F7</accession>
    <name>I1H7F7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100839858</name>
        <name evidence="3" type="ORF">BRADI_1g68070v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558350.1">
        <property type="nucleotide sequence ID" value="XM_003558302.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H7F7"/>
    <dbReference type="STRING" id="15368.I1H7F7"/>
    <dbReference type="EnsemblPlants" id="KQK22566">
        <property type="protein sequence ID" value="KQK22566"/>
        <property type="gene ID" value="BRADI_1g68070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839858"/>
    <dbReference type="Gramene" id="KQK22566">
        <property type="protein sequence ID" value="KQK22566"/>
        <property type="gene ID" value="BRADI_1g68070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839858"/>
    <dbReference type="eggNOG" id="ENOG502QVS3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022742_0_0_1"/>
    <dbReference type="OMA" id="LGWSRAW"/>
    <dbReference type="OrthoDB" id="785851at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042316">
        <property type="entry name" value="IRKI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31029">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31029:SF4">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="134"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK22566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="45925" checksum="E99F5538A4A2A182" modified="2012-06-13" version="1">MVSSPSPSPTFPTVASETKQRAGVATTASDKLDQKYAHSATPLHSHGGGGARKTPRRGKSEGGGADSAAAYVAAVSCSDCRFKQRVLAPASPGAVIRSLFGSLTRRSTPRSSPSPSPTSTASEGDGGEQWRLAAADLSRRLAAATRTRDDAVEETARLKHSLAELELKLARLEARVLPTPSAAAAFPVESFLRAVSTARATVRNLTRALSTHLRSPAAASPGAPNLESFLNRAFHADFELDTDGDAGLHTADPAGRCAANLAAYHAVAALTWEEVLLHGTKHYSEGLSRFCDAKMSEVVSSQGWARARPWPEPLLQAFFLAAKAVWGVRLLAHSVHPPLPVVRVDRGARFDSRFMEDAAAARAGRLEPASVKMMVAPGFHVYVAGAGVVKCKVVCFYNNNGGSRTGGHRDGGSSVNGGVGLGSSCSDVNGGVTVLRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>I1H7S2</accession>
    <name>I1H7S2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100838420</name>
        <name evidence="11" type="ORF">BRADI_1g69080v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558429.1">
        <property type="nucleotide sequence ID" value="XM_003558381.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H7S2"/>
    <dbReference type="FunCoup" id="I1H7S2">
        <property type="interactions" value="2024"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H7S2"/>
    <dbReference type="EnsemblPlants" id="KQK22735">
        <property type="protein sequence ID" value="KQK22735"/>
        <property type="gene ID" value="BRADI_1g69080v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838420"/>
    <dbReference type="Gramene" id="KQK22735">
        <property type="protein sequence ID" value="KQK22735"/>
        <property type="gene ID" value="BRADI_1g69080v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838420"/>
    <dbReference type="eggNOG" id="KOG0936">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="DLIFNWQ"/>
    <dbReference type="OrthoDB" id="10261046at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H7S2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14834">
        <property type="entry name" value="AP3_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000001">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027155">
        <property type="entry name" value="APS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="10">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK22735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="166" mass="18875" checksum="7FED57CAF9C6F455" modified="2012-06-13" version="1">MIQAVMVISTQGKPRLLKFYNFQPPEKHQELVRGVFQLLSARPENVSNFVEADAIFGPGAKLVYKHLATLYFVFIFDSSENELAMLDLVQVFVETLDRCFKNVCELDIVFNFNKLHTILDEMILGGQVIETSSEQIVKSVEEIARLEKQSSTASIIPKSISDRFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1H882</accession>
    <name>I1H882_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100839338</name>
        <name evidence="6" type="ORF">BRADI_1g70330v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558514.1">
        <property type="nucleotide sequence ID" value="XM_003558466.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752589.1">
        <property type="nucleotide sequence ID" value="XM_014897103.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H882"/>
    <dbReference type="STRING" id="15368.I1H882"/>
    <dbReference type="EnsemblPlants" id="KQK22959">
        <property type="protein sequence ID" value="KQK22959"/>
        <property type="gene ID" value="BRADI_1g70330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839338"/>
    <dbReference type="Gramene" id="KQK22959">
        <property type="protein sequence ID" value="KQK22959"/>
        <property type="gene ID" value="BRADI_1g70330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839338"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_10_2_1"/>
    <dbReference type="OMA" id="ARIHPWS"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF24">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="176"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="248"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="283"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="353"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="388"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="424"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="459"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="524"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK22959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="63930" checksum="156AB647EE3A0FA1" modified="2012-06-13" version="1">MAAVWSGCSTSRGCFSQELPRRSGEGTGTRPRNGGDARRVSSSMVMAGGVCVCRAAPCVLESDVTGKEEVVLGILGMGDERPGTGGSDYHKRHGLRRRPARPAVVEKDPARARSVPPASVLESPDKFVHEGSRLHFLEERDEEILSRRLINLSKSNKVRSATELFDSMRASGLQPNAHACNSLLASFVRRGYSTDAMKMFEFMKGKGLATGHTYTLILKAVASTEGYVSALQIFSEIEEGKESRGTLDVIVYNTMISACGRAKDWRQVEKLWRRLVENSISGTLMTYDLLVSTFVQCGQSELAIAAYQEMLLNGLNPSEDIMKAIIASCTKEGRWEFALATFRRMLSAGMKPNIIVFNSIINSLGKAGEDELAFRMYHLLTSSGLEPDQYTWSALLSALYRSGRCWDALELFQGIKSKHSSVLNSHLYNIALMSCERLGQWEHALQLLWMMEKNELQISVVSYNHVIRACEVACEPKVALKVYQRMTHERCSPDTFTHLSVIRACIWGSLWNKVEDILEEVEPDSSIYNTVIQGLCLRGKIRLARKVYTKMRSIGLKPDGKTRSFMLQHIAGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1HC18</accession>
    <name>I1HC18_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100846589</name>
        <name evidence="3" type="ORF">BRADI_2g03290v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT69939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT69941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565338.1">
        <property type="nucleotide sequence ID" value="XM_003565290.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230460.1">
        <property type="nucleotide sequence ID" value="XM_010232158.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754818.1">
        <property type="nucleotide sequence ID" value="XM_014899332.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HC18"/>
    <dbReference type="EnsemblPlants" id="KQK02724">
        <property type="protein sequence ID" value="KQK02724"/>
        <property type="gene ID" value="BRADI_2g03290v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK02725">
        <property type="protein sequence ID" value="KQK02725"/>
        <property type="gene ID" value="BRADI_2g03290v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69939">
        <property type="protein sequence ID" value="PNT69939"/>
        <property type="gene ID" value="BRADI_2g03290v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69941">
        <property type="protein sequence ID" value="PNT69941"/>
        <property type="gene ID" value="BRADI_2g03290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846589"/>
    <dbReference type="Gramene" id="KQK02724">
        <property type="protein sequence ID" value="KQK02724"/>
        <property type="gene ID" value="BRADI_2g03290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02725">
        <property type="protein sequence ID" value="KQK02725"/>
        <property type="gene ID" value="BRADI_2g03290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69939">
        <property type="protein sequence ID" value="PNT69939"/>
        <property type="gene ID" value="BRADI_2g03290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69941">
        <property type="protein sequence ID" value="PNT69941"/>
        <property type="gene ID" value="BRADI_2g03290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846589"/>
    <dbReference type="eggNOG" id="ENOG502QQYR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_405117_0_0_1"/>
    <dbReference type="OMA" id="CDICAAM"/>
    <dbReference type="OrthoDB" id="758104at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HC18">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025486">
        <property type="entry name" value="DUF4378"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044257">
        <property type="entry name" value="TRM32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47071:SF12">
        <property type="entry name" value="OS01G0149900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47071">
        <property type="entry name" value="PROTEIN TRM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14309">
        <property type="entry name" value="DUF4378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4378" evidence="2">
        <location>
            <begin position="660"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK02724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="822" mass="90468" checksum="46A047FDE087A1F1" modified="2012-06-13" version="1">MAQLLHHQDSAFYGKEIPGRRWSIFQFFGLSRRLRSVKMLSEKKHGQDKSPGGSKRRSYVTLKDEDSGVMDDSKNAEVKGKQKGSKKNSGKSSLKSLIAKKLYGKEGEKEKMLPVAPKLLRTLSMHYLERNEYVLDGEAATNGNGSSHGAKMLLQQALSNTLEGSDTDKSSSLLLNRGDEHAKQKSHRSISMDGILHKVPYGHKVSGNKIGEELPRSASVTYDRDGLKPYIGRASKRHVNQGFQRSRSLSESLESYSLLLDSISSSEAKRVLTSSKSTRDHSLDGPGANAFHRTSSSQFRSKGLTTLAEHLVMKVDASESHVADKTVGDGDVNFAVDESSCNQFSDGSKNPVLLEEYLHDKSFHVEVSTEADLCIAPLPSEEHAATCDDDQAPSSTKEVLYTDPPPLEQADIPEEPSMTCDDDQAPSSTKEDLYTDPPPLPLEQVHIPEEPAMTCDDDQAPSSTKEYLYTDPPPLEEDDISEGPSITFDDDQTHSSTEADSSTALPSEDINTEEEHARTSDDTKFQSCTALPSEEINLAEEDEITSYVSKSVEGTCCVPDPIQETEAELNLSCEQETESPTSVLDVAFSYDSATNSEKHAMLDDSSLMPGNDTGDSVDSNVVIASTCEKPSATSLTQEILQEHNSDDLNGLQVDPKNEAELNYVNDIFNKSSFTNETLFDGWCSQNTVALQEEDCQHYEAAAVPLDFTDMCADELLLFDMTNEALLDIYKSYSASKSKASRFSSFERPKPVGDQALRELQSRMSCHLDRPGGTEISAIVFNDLAKADRWINLQRDVDHVGNKLADSVLDKLLTELTLQLAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCB1</accession>
    <name>I1HCB1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DCD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100833476</name>
        <name evidence="5" type="ORF">BRADI_2g04040v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565377.2">
        <property type="nucleotide sequence ID" value="XM_003565329.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCB1"/>
    <dbReference type="FunCoup" id="I1HCB1">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK02843">
        <property type="protein sequence ID" value="KQK02843"/>
        <property type="gene ID" value="BRADI_2g04040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833476"/>
    <dbReference type="Gramene" id="KQK02843">
        <property type="protein sequence ID" value="KQK02843"/>
        <property type="gene ID" value="BRADI_2g04040v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833476"/>
    <dbReference type="eggNOG" id="KOG1072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006099_1_0_1"/>
    <dbReference type="OMA" id="YAWIDDN"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HCB1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013989">
        <property type="entry name" value="Dev_and_cell_death_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044832">
        <property type="entry name" value="NRP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46034">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46034:SF42">
        <property type="entry name" value="OS01G0165200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10539">
        <property type="entry name" value="Dev_Cell_Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00767">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51222">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DCD" evidence="4">
        <location>
            <begin position="42"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK02843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="696" mass="77035" checksum="4B83B7BD081392CD" modified="2012-06-13" version="1">MASSARARMGAGRKTETYNAATPSNTRLHGDQSFSAHNLKEDKLAGVIFGCTHKTINECLSKQLFGLPAGHFTYVKNIKPGLPLFLFNYSDRKMHGIFEAATSGQLAIDQFAWSHDGRTKTLYPAQVHVSTKTQCLPLPENKYKSVISGNYYLFRHFYFELDHAQTRDLISLFVPAPVHPAPNKRSPSVPPAPVHAISNNQFGVSSHSLDPVPYKLVDQNADNASASRTSKNNFDEEASDWDDLDDGLTEKGLDAVSDYRPHINAVHDEQHDTMAILQKLQELSLLRQEKAQSSKDVVSISDKSIPQESPPGATFPKDPSNVTLEGDPVVKDNTSFEQHCGNDELVQIINEIAKKTEAIGKKQIESDQEILVLRKYVKNMKTKLQQLQYQHDKLQMEYSAVLLGETRNIVEGPSIFLIGGHNGISWLPSLDSFYPTIDRLMPLRPMSSARSYTGVAALNDHIYVFGGGDGSSWYNTVECYNRVSNEWMACPRLKQKKGSLAGATLNGKIFAIGGGDGYQSLSEVEMFDPALGSWIYSPFMRQCRFTPAAAELNGVLYVVGGYDFNSNTYLQSMERYDPREGLWTQLASMTTKRGSHSVTVLGEALYAVGGHDGNHMVSTVEIFDPRANSWRLSSPISIPRGYACAVTANDNVYLIGGIETNGENIETVEMYNERQGWSIPGYKAIGQRSFASSIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCS0</accession>
    <name>I1HCS0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100831225</name>
        <name evidence="2" type="ORF">BRADI_2g05410v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565441.1">
        <property type="nucleotide sequence ID" value="XM_003565393.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCS0"/>
    <dbReference type="STRING" id="15368.I1HCS0"/>
    <dbReference type="EnsemblPlants" id="KQK03077">
        <property type="protein sequence ID" value="KQK03077"/>
        <property type="gene ID" value="BRADI_2g05410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831225"/>
    <dbReference type="Gramene" id="KQK03077">
        <property type="protein sequence ID" value="KQK03077"/>
        <property type="gene ID" value="BRADI_2g05410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831225"/>
    <dbReference type="eggNOG" id="ENOG502RXJ5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116951_1_0_1"/>
    <dbReference type="OMA" id="GIFPPWI"/>
    <dbReference type="OrthoDB" id="658712at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35475:SF2">
        <property type="entry name" value="OS01G0184700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35475">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="181" mass="19086" checksum="B3FEF214960FE6C3" modified="2012-06-13" version="1">MAEPDEVAVEPAEESPTTPLLLESAAASPSVEVRLYRQGSGPAAVFRSGLVGPRRDRLDVRGIQADHGLRALFAFNPSASRRGLRIRPDPNRGGLSALPFRDGAAIALDGEPKASWAWTKPAAMVAAAVLAPAAMAAVALYGVPEPLRSSRLANAAFAPWILASVVIVVVRLRTRPRARAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HFQ5</accession>
    <name>I1HFQ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g14240v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HFQ5"/>
    <dbReference type="EnsemblPlants" id="KQK04554">
        <property type="protein sequence ID" value="KQK04554"/>
        <property type="gene ID" value="BRADI_2g14240v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04554">
        <property type="protein sequence ID" value="KQK04554"/>
        <property type="gene ID" value="BRADI_2g14240v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080286_0_0_1"/>
    <dbReference type="InParanoid" id="I1HFQ5"/>
    <dbReference type="OMA" id="NGTGWYK"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05381">
        <property type="entry name" value="CAP_PR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000004">
        <property type="entry name" value="CAP, cysteine-rich secretory protein, antigen 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094556" description="SCP domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="72"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK04554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="210" mass="23341" checksum="91C9C20E5AC248BE" modified="2012-06-13" version="1">MAAAGLIVAVLFLILVPLAAGGFFGIHSNNKNNKNNNEDQESQSPQELEYYAATSKKTGLRSFTHGRGGYKSMPSEFLAAHNAIRARYGVPPLRWSNKLAKYARRWSSKRRIDCLLMHSFASPYGENVFRGTGWDWKAADAVRGWASEASYFDWKAQACYPGHVCGHFTQLVWNDTQLVGCGRAECFFGGVFITCEYEPGGNWKGEAPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HHN4</accession>
    <name>I1HHN4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dof-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100832233</name>
        <name evidence="11" type="ORF">BRADI_2g19930v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566046.2">
        <property type="nucleotide sequence ID" value="XM_003565998.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HHN4"/>
    <dbReference type="STRING" id="15368.I1HHN4"/>
    <dbReference type="EnsemblPlants" id="PNT70923">
        <property type="protein sequence ID" value="PNT70923"/>
        <property type="gene ID" value="BRADI_2g19930v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832233"/>
    <dbReference type="Gramene" id="PNT70923">
        <property type="protein sequence ID" value="PNT70923"/>
        <property type="gene ID" value="BRADI_2g19930v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832233"/>
    <dbReference type="eggNOG" id="ENOG502S4KT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103935_0_0_1"/>
    <dbReference type="OMA" id="LPHADYQ"/>
    <dbReference type="OrthoDB" id="1927254at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HHN4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31089">
        <property type="entry name" value="CYCLIC DOF FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31089:SF82">
        <property type="entry name" value="DOF-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Dof-type" evidence="10">
        <location>
            <begin position="65"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="19"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT70923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="204" mass="21837" checksum="8480BF60E034C3D8" modified="2012-06-13" version="1">MVEMGGGAAAAGFKLFGKVITRQQQQQPPTRASPDSSSSGGGRGSVEQLEEAARRARAAAGARPLPCPRCRSEDTKFCYFNNYNVNQPRHFCRACHRYWTAGGAIRNVPVGSGRRKNRPVLPHADYQVRLAPGPASASASVEAPDMAPALGFIPAPEHGWSSLYVVPSPSPAAAAYRGHGAEMEQCWWLVHAERPPLGSSDPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1HIT6</accession>
    <name>I1HIT6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100846809</name>
        <name evidence="9" type="ORF">BRADI_2g23460v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231310.1">
        <property type="nucleotide sequence ID" value="XM_010233008.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HIT6"/>
    <dbReference type="STRING" id="15368.I1HIT6"/>
    <dbReference type="EnsemblPlants" id="KQK05935">
        <property type="protein sequence ID" value="KQK05935"/>
        <property type="gene ID" value="BRADI_2g23460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846809"/>
    <dbReference type="Gramene" id="KQK05935">
        <property type="protein sequence ID" value="KQK05935"/>
        <property type="gene ID" value="BRADI_2g23460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846809"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_0_1"/>
    <dbReference type="OMA" id="TAWYYGT"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19125">
        <property type="entry name" value="AKR_AKR4C1-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000010">
        <property type="entry name" value="NADPH-dependent aldo-keto reductase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044498">
        <property type="entry name" value="AKR4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="8">
        <location>
            <begin position="16"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="7">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK05935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="311" mass="34401" checksum="0B012F049E3DC654" modified="2012-06-13" version="1">MAESFVLNTGARIPSIGFGTWQIEPAVVGHTIYAAVKAGYRHIDCAPIYRNEKEVGLALKKLFEDGVVKREDMFITSKLWAGDHAPEDVPEAIDTTLKDLQLDYLDLFLIHGPIRIKKGTMPSPDNFLPPDIPATWGAMEKLYDSGKARAIGVSNFSCKKLEDLLSIARVPPAVNQVECHPIWQQYKLRKLCRSRGVHLSASSPLGSPASFGVSGANVLSNPIVISVAEKLGKTPAQVALRWGIQMGQSVLPKSAHEARIKENYDIFCWSIPEDLLAKFSDIKQARLLRAEFAVHPLSVYKTLEDLWDGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1HNP4</accession>
    <name>I1HNP4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Male-enhanced antigen 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100837683</name>
        <name evidence="2" type="ORF">BRADI_2g41350v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569224.1">
        <property type="nucleotide sequence ID" value="XM_003569176.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HNP4"/>
    <dbReference type="STRING" id="15368.I1HNP4"/>
    <dbReference type="EnsemblPlants" id="KQK08353">
        <property type="protein sequence ID" value="KQK08353"/>
        <property type="gene ID" value="BRADI_2g41350v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837683"/>
    <dbReference type="Gramene" id="KQK08353">
        <property type="protein sequence ID" value="KQK08353"/>
        <property type="gene ID" value="BRADI_2g41350v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837683"/>
    <dbReference type="eggNOG" id="KOG0760">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135252_0_0_1"/>
    <dbReference type="OMA" id="PPWAGSV"/>
    <dbReference type="OrthoDB" id="1933769at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37175">
        <property type="entry name" value="BNAA08G28800D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37175:SF1">
        <property type="entry name" value="CONSTANS-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06910">
        <property type="entry name" value="MEA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK08353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="16473" checksum="B945D9054729A804" modified="2012-06-13" version="1">MSIGNGHLPASTGLPDHSSASDSEPESNTEYLPITGSAASDTDTDTDTEPDPDLASHHRLGAIGNGMSGLDLASDNEDANDGGEADEEEEQCPAEAAAARALSEDERRRRAPLPADAAARIVDAMRGVAFPGAPPSWADSIPDDQWLDRLRSLRGGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPT6</accession>
    <name>I1HPT6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100834600</name>
        <name evidence="8" type="ORF">BRADI_2g44880v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233470.2">
        <property type="nucleotide sequence ID" value="XM_010235168.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPT6"/>
    <dbReference type="STRING" id="15368.I1HPT6"/>
    <dbReference type="EnsemblPlants" id="KQK08922">
        <property type="protein sequence ID" value="KQK08922"/>
        <property type="gene ID" value="BRADI_2g44880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834600"/>
    <dbReference type="Gramene" id="KQK08922">
        <property type="protein sequence ID" value="KQK08922"/>
        <property type="gene ID" value="BRADI_2g44880v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834600"/>
    <dbReference type="eggNOG" id="KOG1021">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025166_3_1_1"/>
    <dbReference type="OMA" id="RREPSYW"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF59">
        <property type="entry name" value="EXOSTOSIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="136"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="47"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK08922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="473" mass="53617" checksum="781C7F70FC98DDE6" modified="2012-06-13" version="1">MVSASCCRVVGLGSLLAAAAATTAFLTFSLPSSNGVSTAKLTVAVSVNASSTPPPPPPPPPPTLATALPPPPPPPPPVARPRKREPSYGRMTPEEALRYAKKEIMAAEPVVNDPDLYAPLFRNVSQFKRSYELMERILKVYIYQDGRRPIFHTPPLSGIYASEGWFMKLLKESRRFVVADGAKAHLFYLPYSSQHLRLSLYVPDSHNLRPLAVYLRDFVQGLAAKYPFWNRNRGADHFLVACHDWGPYTTTAHRDLRRNSIKALCNADSSERIFSPGKDVSLPETTIRTPKRPLRYVGGLPVSRRRILAFFAGNVHGRVRPVLLKHWGDGRDDDMRVYGPLPNRVSRQMSYIQHMKNSRFCLCPMGHEVNSPRIVEALYYECVPVVIADNFVLPFSDVLDWTAFSVVVAEKDIPDLKKILQGISLRRYVAMHDCVKRLQRHFLWHARPLRYDLFHMILHSIWLSRVNHVELLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQ49</accession>
    <name>I1HQ49_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Magnesium transporter CorA-like family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100821306</name>
        <name evidence="8" type="ORF">BRADI_2g45840v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569508.1">
        <property type="nucleotide sequence ID" value="XM_003569460.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQ49"/>
    <dbReference type="STRING" id="15368.I1HQ49"/>
    <dbReference type="EnsemblPlants" id="KQK09069">
        <property type="protein sequence ID" value="KQK09069"/>
        <property type="gene ID" value="BRADI_2g45840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821306"/>
    <dbReference type="Gramene" id="KQK09069">
        <property type="protein sequence ID" value="KQK09069"/>
        <property type="gene ID" value="BRADI_2g45840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821306"/>
    <dbReference type="eggNOG" id="ENOG502QR0C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024835_1_0_1"/>
    <dbReference type="OMA" id="VQHKSWP"/>
    <dbReference type="OrthoDB" id="1931410at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46950">
        <property type="entry name" value="MAGNESIUM TRANSPORTER CORA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46950:SF10">
        <property type="entry name" value="OS01G0664100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="475"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="532"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK09069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="62812" checksum="9B7DA6B7CF70C9E2" modified="2012-06-13" version="1">MTDVEGQPPPQISSSMSKVQDGSGGNPNLAKECHILGSELWTNGLICAFELVKGHRKIVHHKSWPAIELAQEKVVHMKKHRGRDGHHVVNPTPDESNVVEIPGQTELGNDPSVLKDRPPYPAEILDHKWVPIGWSRIGELIQRVQSDASWENEQVMISDSEDDYTVADVAAPYWQRPGGPTWWFHVTAGHPYVDAWLSSAHWMHPAIRTALRDESRLISDRMKYLLYEVPVRVAGGLLFELLGQSVGDPNHEEEDIPIVLRSWQAQNFLLTAMHVKGHSPNINVLGVTEVQELLISDGSQTPRSVHEVIAHLVSRLSRWDDRLFRKYIFGKADEIELKFVNRRNSEDLNLVSIILNQEIRRLAKQVIRVKWSLHAREEIILELLRHLRGNATRAILERERKSAREMLEEQEAVRGRLFTIQDVMQSTVRAWLQDRSLRITHNLAVFGGGGIVLSIITGLFGINVDGIPGAQNTPYAFGLFAGLLFFIGAVLVGVGLMYLGLTKPVTSEKVNVRKLELQQLVSVFQREAEQHGKVREGLSRHGLSSGSSSASPGEGYILIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HTW0</accession>
    <name>I1HTW0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5'-3' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100839076</name>
        <name evidence="5" type="ORF">BRADI_2g56537v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232630.1">
        <property type="nucleotide sequence ID" value="XM_010234328.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HTW0"/>
    <dbReference type="STRING" id="15368.I1HTW0"/>
    <dbReference type="EnsemblPlants" id="KQK10838">
        <property type="protein sequence ID" value="KQK10838"/>
        <property type="gene ID" value="BRADI_2g56537v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73286">
        <property type="protein sequence ID" value="PNT73286"/>
        <property type="gene ID" value="BRADI_2g56537v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839076"/>
    <dbReference type="Gramene" id="KQK10838">
        <property type="protein sequence ID" value="KQK10838"/>
        <property type="gene ID" value="BRADI_2g56537v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73286">
        <property type="protein sequence ID" value="PNT73286"/>
        <property type="gene ID" value="BRADI_2g56537v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPWK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064190_0_0_1"/>
    <dbReference type="OMA" id="YKAHRPR"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HTW0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000042">
        <property type="entry name" value="5'-3' exonuclease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000027">
        <property type="entry name" value="5'-3' exonuclease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038969">
        <property type="entry name" value="FEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42646:SF4">
        <property type="entry name" value="5'-3' EXONUCLEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42646">
        <property type="entry name" value="FLAP ENDONUCLEASE XNI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="5'-3' exonuclease" evidence="4">
        <location>
            <begin position="79"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="30"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK10838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="366" mass="40343" checksum="6ED82E958BA1CFE8" modified="2012-06-13" version="1">MLMQLPAVGAATAAPLAPVKAATAASPRRYPPIRLSASPSTESSPSTSAVSGQRLPPSARHSREHLGGRNNVPARPSKGRVFFLDVNPICFRGSQRSLGAFARWLSLFFAHVSLRDPVVAVLDGVGGNEYRRRLLPSYKAHRTRGVGKGADSRVVDVLRECNVPVVQIDGHEADDVVATLTEQVLQKGFRVVIGSPDKDFKQLISEDVQLVMPIPEIGRWSFYTLRHYVAQYKCNPTADLSLRCFMGDEADGVPGIQHLVPGFGRKTAVKLLKKHGTLENLLNTAAIKTVGKDYAQDALVKHADYLRKNYEVLSLKRDVNVQLDDRWLSTRDTCNDTSVLSNFILKFNEGQKLNSRFRTGTDRSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1HTX6</accession>
    <name>I1HTX6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SnRK1-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100842020</name>
        <name evidence="2" type="ORF">BRADI_2g56682v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564758.1">
        <property type="nucleotide sequence ID" value="XM_003564710.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HTX6"/>
    <dbReference type="STRING" id="15368.I1HTX6"/>
    <dbReference type="EnsemblPlants" id="KQK10861">
        <property type="protein sequence ID" value="KQK10861"/>
        <property type="gene ID" value="BRADI_2g56682v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842020"/>
    <dbReference type="Gramene" id="KQK10861">
        <property type="protein sequence ID" value="KQK10861"/>
        <property type="gene ID" value="BRADI_2g56682v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842020"/>
    <dbReference type="eggNOG" id="ENOG502RXQN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095139_1_0_1"/>
    <dbReference type="OMA" id="RTWSWET"/>
    <dbReference type="OrthoDB" id="1935514at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037489">
        <property type="entry name" value="RAD52-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34050:SF1">
        <property type="entry name" value="DNA REPAIR RAD52-LIKE PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34050">
        <property type="entry name" value="DNA REPAIR RAD52-LIKE PROTEIN 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK10861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="20334" checksum="C8BDB4041BE7F9FB" modified="2012-06-13" version="1">MAPGALARLIIGRRATGPLLARPFAAKARASRQAPEPEPLSEEEEDDFSGGVEAAPTEGISKPLADVLRELGKRVPDSLVKTRLEDNGFALKYIPWHIVNKILNVHAPEWSGEVRNIIYSSDGKSVSVVYRVTLHGTDAEIYREATGTASVQDTGFGDPVQKAEGMAFRRACARLGLGLHLYHEDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUI1</accession>
    <name>I1HUI1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100835313</name>
        <name evidence="3" type="ORF">BRADI_2g58520v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564903.1">
        <property type="nucleotide sequence ID" value="XM_003564855.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUI1"/>
    <dbReference type="FunCoup" id="I1HUI1">
        <property type="interactions" value="391"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK11165">
        <property type="protein sequence ID" value="KQK11165"/>
        <property type="gene ID" value="BRADI_2g58520v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835313"/>
    <dbReference type="Gramene" id="KQK11165">
        <property type="protein sequence ID" value="KQK11165"/>
        <property type="gene ID" value="BRADI_2g58520v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835313"/>
    <dbReference type="eggNOG" id="ENOG502SGWY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145659_0_0_1"/>
    <dbReference type="OMA" id="DSMVSNC"/>
    <dbReference type="OrthoDB" id="656626at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04660">
        <property type="entry name" value="nsLTP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039265">
        <property type="entry name" value="DIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044741">
        <property type="entry name" value="NsLTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122">
        <property type="entry name" value="LIPID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122:SF62">
        <property type="entry name" value="OS01G0914300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094823" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="34"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK11165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="114" mass="11639" checksum="05172B9D6E79B79C" modified="2012-06-13" version="1">MAGIRKASSSAAAMAMALLVVAALLLLVAVAGAAGRHTACHVDLERMEDKCGSYCAAGSREGSPSGECCDAVRGADFPCLCRYKPLLRSVAPGMDANRAMQIPAKCGIPNVPAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXS4</accession>
    <name>I1HXS4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100825210</name>
        <name evidence="11" type="ORF">BRADI_3g05480v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570992.1">
        <property type="nucleotide sequence ID" value="XM_003570944.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXS4"/>
    <dbReference type="FunCoup" id="I1HXS4">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93574">
        <property type="protein sequence ID" value="KQJ93574"/>
        <property type="gene ID" value="BRADI_3g05480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825210"/>
    <dbReference type="Gramene" id="KQJ93574">
        <property type="protein sequence ID" value="KQJ93574"/>
        <property type="gene ID" value="BRADI_3g05480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825210"/>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000837_25_4_1"/>
    <dbReference type="OMA" id="KEYPREW"/>
    <dbReference type="OrthoDB" id="1517790at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1052">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="173"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="436"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="606"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ93574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="921" mass="104493" checksum="927F022CD5181984" modified="2012-06-13" version="1">MAEAVMGPLVGRLQELAMSEARAMVAVNDDVRSLRDKLMWMQAFLREAEPRRRAKNDELIRVCLQQTRDAVFDAEDAVDHYFLQVDLSRYPNWSHAILKFVSGFTTQVRVRHHLSRRIKSINTRLEGIVENKGKYKIDDTTDDNSITTWRPSTAISAITENMSDFVLPLVGRETKIEELGDALFDKQSVVPVVICVTGKSGIGKTKLVKEIYKKPLTKRNFDVQVWVTCAPNLSATNIKKLILQRMTKDTVRSPTEQLQRKLGNKKYLVVIDGETSNTEWKKILLDLPKDKTESRVVKITHATKQETTVSGFKQHIIELNPIGIKDVTNMFMNTLLMDEKVGKKSRGQVEEALTRDGDGSYINANRIFKVTEGLPLAVVLLSGLLRTKEYPREWKKVFDHLEGKSNEWKPLDIILSMCLDDLPHDLKSCFLYFAGFPASTLVKARSLVCMWMAEGLLRPKEGKTMEKVGKKYLHELLCRGLMNFPPLENATPGNERVTVQTKVHEFLLIEAQEANFMEIHDGDDVPTLSNARRLTLQNHKDKYAALTDPLPKLRSIMSNFEKDDSQGSVAETKEEEEIAGANACSPIPLCLRPHKDRVNSKDHMRKLLQGSQFLRVICLCGLEVGSKLPSEIGSLVHLQYLGITYCFLNEIPSSVGNLTRLQTLDVQGTSVTKLPQELWRIPTLRHVFGFIVLPRRVGDLEQLQTLEAVKPDDAGVTASDSCWDAKTFANMKRLHSLYIWDISNKNVKCLEAVYGLKYLVLLSIQGKVISLDLFTRSKLSRLQEMVLKGKIVAPTTPLTASNRFFFPTLTKLSLNKTKVSKDFIDRLSEDLPLLATLALFRDSYSESCLVFTNGFHSLKELTLDVYLEEIVIKEEACPDLMKLEVVVHYLNLCIDIPNKPSLKDTIQDMMKQRTIEVNRDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXT1</accession>
    <name>I1HXT1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Jasmonate O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100827382</name>
        <name evidence="4" type="ORF">BRADI_3g05550v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570381.1">
        <property type="nucleotide sequence ID" value="XM_003570333.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233886.1">
        <property type="nucleotide sequence ID" value="XM_010235584.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233887.1">
        <property type="nucleotide sequence ID" value="XM_010235585.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233888.1">
        <property type="nucleotide sequence ID" value="XM_010235586.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233889.1">
        <property type="nucleotide sequence ID" value="XM_010235587.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXT1"/>
    <dbReference type="STRING" id="15368.I1HXT1"/>
    <dbReference type="EnsemblPlants" id="KQJ93584">
        <property type="protein sequence ID" value="KQJ93584"/>
        <property type="gene ID" value="BRADI_3g05550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93585">
        <property type="protein sequence ID" value="KQJ93585"/>
        <property type="gene ID" value="BRADI_3g05550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93586">
        <property type="protein sequence ID" value="KQJ93586"/>
        <property type="gene ID" value="BRADI_3g05550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827382"/>
    <dbReference type="Gramene" id="KQJ93584">
        <property type="protein sequence ID" value="KQJ93584"/>
        <property type="gene ID" value="BRADI_3g05550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93585">
        <property type="protein sequence ID" value="KQJ93585"/>
        <property type="gene ID" value="BRADI_3g05550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93586">
        <property type="protein sequence ID" value="KQJ93586"/>
        <property type="gene ID" value="BRADI_3g05550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827382"/>
    <dbReference type="eggNOG" id="ENOG502QQVK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019628_2_0_1"/>
    <dbReference type="OMA" id="ASEFTHA"/>
    <dbReference type="OrthoDB" id="1523883at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ93584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="39969" checksum="1270F6C92DD6F4B2" modified="2012-06-13" version="1">MSSEQMVHMNRGQGETSYAQNSSLQNAAQNRMRSLIEEAIADLCSTSTLLPSRSMVVADLGCSSGPNALVLVSIAVDAIQSHCLRYQQQPLAEICVLLNDLPDNDFNVVVKSLVAFQQSHKSIVAGIVPGSFYGRLFCSDSLHLVCSSNSLHWLSKAPDELKRNRIPAYDIDEHVRRERRTIVLGAYARQFGKDFTQFLELRAKELVPGGRMVVSLAGRRSEEPASKYTHAWESVAQILSEMASKGVINRGKFESFYIPIYGPSDEGLRDIIQSEGSFSIRELQVHEPTSDNTLITPSRMANMLRAGFEPIIIQHFGPAETIMDKFVSTAERRWSLQGSLQEELAANPRVILVVSLTKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYX4</accession>
    <name>I1HYX4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100822442</name>
        <name evidence="13" type="ORF">BRADI_3g08557v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for the accumulation of coenzyme A in the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234056.1">
        <property type="nucleotide sequence ID" value="XM_010235754.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYX4"/>
    <dbReference type="FunCoup" id="I1HYX4">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HYX4"/>
    <dbReference type="EnsemblPlants" id="KQJ94107">
        <property type="protein sequence ID" value="KQJ94107"/>
        <property type="gene ID" value="BRADI_3g08557v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822442"/>
    <dbReference type="Gramene" id="KQJ94107">
        <property type="protein sequence ID" value="KQJ94107"/>
        <property type="gene ID" value="BRADI_3g08557v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822442"/>
    <dbReference type="eggNOG" id="KOG0752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="MAKRENG"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HYX4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990559">
        <property type="term" value="P:mitochondrial coenzyme A transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000014">
        <property type="entry name" value="mitochondrial coenzyme A transporter SLC25A42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="112"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="218"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ94107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="36339" checksum="8244F765C12A3F07" modified="2012-06-13" version="1">MEAAESGGGGGGGALPLAVRELIAGGVAGGVAKTAVAPLERVKILLQTRRAEFHGSGLVGSSRTIYRTEGPLGFYRGNGASVARIVPYAALHYMAYEEYRRWIILAFPNVEQGPILDLVAGSIAGGTAVICTYPLDLVRTKLAYQIKGAVNLSLIESKPSEQVYKGILDCVKTIYKQNGLKGLYRGMAPSLYGIFPYSGLKFYFYEKMKTHVPEEHRKDITTKLACGSVAGLLGQTITYPLDVVRRQMQVQAFSSSNLAKGKGTFGSLVMIAKHQGWQQLFSGLSINYLKVVPSVAIGFTVYDSMKDWLNVPSREQTAVNVPVLTEDGSNAAHVHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1I161</accession>
    <name>I1I161_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100822456</name>
        <name evidence="3" type="ORF">BRADI_3g15730v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573419.1">
        <property type="nucleotide sequence ID" value="XM_003573371.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I161"/>
    <dbReference type="EnsemblPlants" id="KQJ95201">
        <property type="protein sequence ID" value="KQJ95201"/>
        <property type="gene ID" value="BRADI_3g15730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822456"/>
    <dbReference type="Gramene" id="KQJ95201">
        <property type="protein sequence ID" value="KQJ95201"/>
        <property type="gene ID" value="BRADI_3g15730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822456"/>
    <dbReference type="eggNOG" id="ENOG502T1DZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014546_7_2_1"/>
    <dbReference type="OMA" id="MELLHWM"/>
    <dbReference type="OrthoDB" id="1862401at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051504">
        <property type="entry name" value="Plant_metabolite_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31625">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ95201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="454" mass="47819" checksum="5F5983B19091EEEB" modified="2012-06-13" version="1">MPTVNVVEVARVAPSAPQPEDGPEGSLLLGLSALEVPWIALPLIQRVLIFVDNADADGGVQQLPPFASMVASLRASLAATLARFPPLAGRIVHLPSTADAAIDCSASDGVRFMVAESEDADAARLVGEADHDVGAFRELVPELETGALPAEVLAVQVTRLNGGAAVGVAMHHAVVDGRSVWRFLAAWAAACRGDGDDAVAPAFERAVVAVPGGEELVRSTLRKYSPNLPLTTMIIPGSGPTLPRRTFTVTAQHIHRLKQRITDDLTSSPTPYSSFVALAALTWVSFVRSKHPSAISSSSSSVYLFFFVDCRGRPGIEPPVPESYFGTCISGALAKATARDLLAEDGLAFAAAAVQAEVRRAAEDPLALWDWMELLHWMPLDRLVNVSGSARFPAYEAADFGWGPPGRTELVTMNAGGQLVLVAAKGGAGAVQASVCMEAEHMDCFNSHFLGLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2F4</accession>
    <name>I1I2F4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100832341</name>
        <name evidence="5" type="ORF">BRADI_3g19360v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571594.1">
        <property type="nucleotide sequence ID" value="XM_003571546.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2F4"/>
    <dbReference type="FunCoup" id="I1I2F4">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="MEROPS" id="A01.A07"/>
    <dbReference type="EnsemblPlants" id="KQJ95841">
        <property type="protein sequence ID" value="KQJ95841"/>
        <property type="gene ID" value="BRADI_3g19360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832341"/>
    <dbReference type="Gramene" id="KQJ95841">
        <property type="protein sequence ID" value="KQJ95841"/>
        <property type="gene ID" value="BRADI_3g19360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832341"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005738_5_2_1"/>
    <dbReference type="OMA" id="KMTGEHK"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05472">
        <property type="entry name" value="cnd41_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000021">
        <property type="entry name" value="Aspartyl protease AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000049">
        <property type="entry name" value="Aspartyl protease AED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033873">
        <property type="entry name" value="CND41-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF827">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095056" description="Peptidase A1 domain-containing protein" evidence="3">
        <location>
            <begin position="34"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="180"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ95841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="533" mass="55212" checksum="4C3AE0353B5BFE4E" modified="2012-06-13" version="1">MVAASSSSWSPVQHHLLLPVLIALLAFVGHVAAAAGSSDARNAVVGKKSAVLSLRELEYWGTGTAAARETIQGRRYAQAKQAGFLAGEDKKAAEEPAARRSRSTTAVLELKHHSSTATVPDHPAARERYLKHLLAADSARAASLQLRKPKPASSTTTTQASAAAAEVPLGSGIRYQTLNYVTTIALGGGGAKNLTVIVDTGSDLTWVQCEPCPGSSCYAQRDPLFDPAASPTFAAVPCGSPACAASLKDATGAPGSCARSAGNSEQRCYYALSYGDGSFSRGVLAQDTLGLGTTTKLDGFVFGCGLSNRGLFGGTAGLMGLGRTDLSLVSQTAARFGGVFSYCLPATTTSTGSLSLGPGPSSSFPNMAYTRMIADPTQPPFYFINITGAAVGGGAALTAPGFGAGNVLVDSGTVITRLAPSVYKAVRAEFARRFEYPAAPGFSILDACYDLTGRDEVNVPLLTLTLEGGAQVTVDAAGMLFVVRKDGSQVCLAMASLPYEDQTPIIGNYQQRNKRVVYDTVGSRLGFADEDCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1I3G8</accession>
    <name>I1I3G8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100837888</name>
        <name evidence="3" type="ORF">BRADI_3g22830v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234658.1">
        <property type="nucleotide sequence ID" value="XM_010236356.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I3G8"/>
    <dbReference type="FunCoup" id="I1I3G8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ96389">
        <property type="protein sequence ID" value="KQJ96389"/>
        <property type="gene ID" value="BRADI_3g22830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837888"/>
    <dbReference type="Gramene" id="KQJ96389">
        <property type="protein sequence ID" value="KQJ96389"/>
        <property type="gene ID" value="BRADI_3g22830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837888"/>
    <dbReference type="eggNOG" id="ENOG502RMG6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014546_4_0_1"/>
    <dbReference type="OMA" id="VNSRKYV"/>
    <dbReference type="OrthoDB" id="444127at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF53">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ96389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="448" mass="47244" checksum="ECA440A0BCF0D570" modified="2012-06-13" version="1">MSVSVSKSSPVVVRPSSEPATSTAGNLLKLSSYDKNHMCIPVTMFLVFDNPINEPAETIKSALSQALVPYLPFSGRLAAENNAVHITFGGDDQGVSFVAAAASCGLKEVDLSDRSSPLTSTLLDELAVYYPAAACGFGDPLLLMQVTEFTCGGFVVGVTWNHVIADAAGMGQFLRAVGERARGLTLASSVVPVRWDAALKAAPPAAAGFVELMMGLKPMADVAILDVTVPSRLIGSVKDAFRRRGKSPCTTFEAVAAVLWRCRTRAILSGSSSDSSEINPVVALYFTVNARNYAGAREGYYGNCVTGRFVVATGGTVANGDLVDVVEMIRRAKEQIPEQFKEGAGIGNGNDGVRQQQVNVDGLVGYNLFIVTCWRNLGVDEADFGGGRPARVTSHTRQEVTAPACVACPPFRGNDGANVLSGCVRKEHADAFLAELALSSRTPALLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1I4E2</accession>
    <name>I1I4E2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100833403</name>
        <name evidence="7" type="ORF">BRADI_3g27852v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1I4U2</accession>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="3">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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    </evidence>
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        <source>
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    <sequence length="197" mass="20863" checksum="B17E44AEADE715BB" modified="2012-06-13" version="1">MADRLLLLVLVAAAALSVLVPSPVAAARPCHTLLVSYSANANPSNDPDHRAPLTATVLTIFRVRRFGSHLLLRAKPGGHAHPHPDHRHHHHLHSIPANIQIRRPELPHPAAGAAAGIQERVKDILVIVVGILFGVGCGALTAASMYLVWSVIAGPGASSNYDGLYGDEEDDASDYESPKKVGYVIIPGMEAQDGGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1I676</accession>
    <name>I1I676_BRADI</name>
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        </recommendedName>
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        <name type="synonym">Trachynia distachya</name>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003572041.4"/>
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    <dbReference type="STRING" id="15368.I1I676"/>
    <dbReference type="EnsemblPlants" id="KQJ97825">
        <property type="protein sequence ID" value="KQJ97825"/>
        <property type="gene ID" value="BRADI_3g33537v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834514"/>
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        <property type="protein sequence ID" value="KQJ97825"/>
        <property type="gene ID" value="BRADI_3g33537v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834514"/>
    <dbReference type="eggNOG" id="KOG1167">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001725">
        <property type="entry name" value="Kinase like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001893">
        <property type="entry name" value="Probable serine/threonine-protein kinase DDB_G0291918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF23">
        <property type="entry name" value="CDC7 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="494"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="676"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="793"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="713"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ97825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1002" mass="109783" checksum="424586240C6415F5" modified="2012-06-13" version="1">MDPAGVGPRETELAWHLLTVLVRIGRPAAAAELAATVASASGDVTPQLIERLCGAPGSPLRCSSGGVVTASETAVAAFLRFVGWEYVPPPPRVGLRASDLRRWRGEVPIRYDCKGKASSDAKDFGVKRRLLMGGDSDLVEQEQQQLQQLIVQSCSSVATSEVHLEVMQEGIPNLGPYTVEPSLKFLTGGTLVPSAAQISMSCLQHRLDQLFGGSDENILGKTTSAPVPMEVSTPSFIGIPSLCAEKTERVHAAADNETNGIGEPEGAFPFYNSRVEDSDDLEKASLLPTTAAVGTTVIGVDEDLNFIYKGPSSPFHNCNTHAVENTGRSNVISDQAAVRHDSPNAGYCEEIPSCGPNANASAEIQNNKAIQVLFQSPTHTKAEPILHDRMHRTIQSSCQPSLDAKVKPAVLPLEATSYDCVKNSIENKYLIDREQQRNDVGVNFSKKEQDRKIVKQRDKGKKNNVLPAGVKDQLAERAQKGNAEPKPLPIFKNFVVEEEEGSGGYGIVYRARRKTDGSIVAIKCPHSNAHSHHVDNERKMLERFGGKHFVIRYETSLRSGDLDCFVLEHVVHDRPEILKKEISVFEMKWYGYCLFSALSSLHKQGVVHRDVKPGNFLFSRRLAKGYLIDFNLANDIHQKYLRNSKSETISCGKDTTSQSALKSALVIHAKEVAADPKQALGSKRKRSNKSPVGGDPRIGNKSKHGGQAADISGVTSAKDPTSTKTQLDRLKQPMPYKGRKELMNFLHEAMHSPKQKTLEAPVSQRKRVAAPVSSGDRKLFVLTPMPLRSGGSAVAGTGMFNSKGQGKQRREGPCVGTKGFRAPEVLLRSFYQGCKVDVWSAGVTLLYLIIGKSPFGGDPEQNMKEIAKLRGSEGLWEVAKLHNCESSYPSELCDAKWLQSVDLREWCVANARRPEFLEYLPDSLFDLVDKCLAVNPRCRITSDEALSHEFFASCHESLRKKKALRIGRSGAGASHPPPSLSQDNMVKVNESQRLFPTIFDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8S2</accession>
    <name>I1I8S2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100829918</name>
        <name evidence="8" type="ORF">BRADI_3g40830v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572421.3">
        <property type="nucleotide sequence ID" value="XM_003572373.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8S2"/>
    <dbReference type="FunCoup" id="I1I8S2">
        <property type="interactions" value="1184"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I8S2"/>
    <dbReference type="EnsemblPlants" id="KQJ99063">
        <property type="protein sequence ID" value="KQJ99063"/>
        <property type="gene ID" value="BRADI_3g40830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829918"/>
    <dbReference type="Gramene" id="KQJ99063">
        <property type="protein sequence ID" value="KQJ99063"/>
        <property type="gene ID" value="BRADI_3g40830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829918"/>
    <dbReference type="eggNOG" id="KOG4293">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036675_2_0_1"/>
    <dbReference type="OMA" id="EICKYEN"/>
    <dbReference type="OrthoDB" id="2419613at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd09629">
        <property type="entry name" value="DOMON_CIL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045265">
        <property type="entry name" value="AIR12_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130:SF89">
        <property type="entry name" value="OS08G0524400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04526">
        <property type="entry name" value="DUF568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095184" description="DOMON domain-containing protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="7">
        <location>
            <begin position="47"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="198"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ99063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="257" mass="25327" checksum="BA933BF2C71B1A5D" modified="2012-06-13" version="1">MPPHHLALLFLVLLLSSPTLSLAASACASEKFPAGRKYANCEDLPQLGAALHWTYDDATAALSLAFVAAPAKPGGWVAWGLNPIGSGMAGAQALVALRPSPSAPVAVRTYNITGYVPLGGDSTPLAFPATELAADEESGGKIIRVYGKLQLRKGMKEVSQVWQVGPSVSKGAPDKHDVAAGNLAAKATLVLAGGSGKAAAGPAPAPTSGGGPDAGEGAGTVTAPAAGTTTPSASASAAGMSSATFLVLASAAAFFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1I910</accession>
    <name>I1I910_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100843984</name>
        <name evidence="10" type="ORF">BRADI_3g41530v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C65 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572459.3">
        <property type="nucleotide sequence ID" value="XM_003572411.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I910"/>
    <dbReference type="STRING" id="15368.I1I910"/>
    <dbReference type="EnsemblPlants" id="PNT68507">
        <property type="protein sequence ID" value="PNT68507"/>
        <property type="gene ID" value="BRADI_3g41530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843984"/>
    <dbReference type="Gramene" id="PNT68507">
        <property type="protein sequence ID" value="PNT68507"/>
        <property type="gene ID" value="BRADI_3g41530v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3991">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014832_3_1_1"/>
    <dbReference type="OMA" id="GARACHQ"/>
    <dbReference type="OrthoDB" id="18915at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I910">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061815">
        <property type="term" value="F:Met1-linked polyubiquitin deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22765">
        <property type="entry name" value="AtOTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.20:FF:000001">
        <property type="entry name" value="Ubiquitin thioesterase OTUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.60">
        <property type="entry name" value="Peptidase C65 Otubain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.20">
        <property type="entry name" value="Peptidase C65 Otubain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042468">
        <property type="entry name" value="Peptidase_C65_otubain_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042467">
        <property type="entry name" value="Peptidase_C65_otubain_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF15">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="9">
        <location>
            <begin position="196"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="100"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="108"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT68507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="416" mass="46113" checksum="DD681DE4638C494B" modified="2012-06-13" version="1">MRRAYLRKRQAVNAEISGRRKQKKKKKNRSPESALFSLSLSPSRIEAVVSLRLREPDPQEAKLAREAADASARKRKADGGEDAGADAAAAPVPEEVAMGDAAAPVPDPEPRVEGGGEGADPKPSRCSPEPVSVELSMGGDYYYRACCGDPDPDPKPEGPQVPYVGDKEPLSALAAEFQSGSPILQEKIKLLGEQYDALRRTRGDGNCFYRSFMFSYLEHILETQDKSEVERIQKNIEQCKKTLIGLGYIEFTFEDFFSIFIEQLENVLQGHETSIGPEDLLERTRDQMVSDYAVMFFRFVTSGEIQRRTEFFEPFISGLTNSTVAQFCKASVEPMGEESDHVHIIALSDALGVPIRVMYLDRSSCDPGNLSVNHHDFVPAANSSEGDASMTSTPADEKPYITLLYRPGHYDILYPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBA4</accession>
    <name>I1IBA4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100836273</name>
        <name evidence="11" type="ORF">BRADI_3g47960v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575300.1">
        <property type="nucleotide sequence ID" value="XM_003575252.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBA4"/>
    <dbReference type="STRING" id="15368.I1IBA4"/>
    <dbReference type="EnsemblPlants" id="KQK00211">
        <property type="protein sequence ID" value="KQK00211"/>
        <property type="gene ID" value="BRADI_3g47960v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68978">
        <property type="protein sequence ID" value="PNT68978"/>
        <property type="gene ID" value="BRADI_3g47960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836273"/>
    <dbReference type="Gramene" id="KQK00211">
        <property type="protein sequence ID" value="KQK00211"/>
        <property type="gene ID" value="BRADI_3g47960v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68978">
        <property type="protein sequence ID" value="PNT68978"/>
        <property type="gene ID" value="BRADI_3g47960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836273"/>
    <dbReference type="HOGENOM" id="CLU_007920_1_0_1"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IBA4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05506">
        <property type="entry name" value="Bromo_plant1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052442">
        <property type="entry name" value="Env_Response_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037377">
        <property type="entry name" value="GTE_bromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46136:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR GTE11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46136">
        <property type="entry name" value="TRANSCRIPTION FACTOR GTE8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="9">
        <location>
            <begin position="175"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="10">
        <location>
            <begin position="302"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="382"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="436"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="592"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="647"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="397"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK00211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="705" mass="77507" checksum="D0FD8B5A7582F049" modified="2012-06-13" version="1">MTPTVLMECGPQRQIKRGYDEMTCRGVAAAPPRGYAETVGESGSPVRVDSEDSSAPKRKCISLNSDGFDVKREIFVPSKMSSSERRYLRKRFRAELDSVRDLLKKPEFLVTMPASRAPAFSSSAAPRPKKVQRGSHVVRGAKGRFLPTKPRPETTTVLPEAAVLKQCEAILKKLMTQKYSHIFNVPVDVEKLNIPDYNDIVKHPMDLGTIKKKLDSGSYTSPSSFAADVRLTFNNAITYNPRGHAVHDMAIQLNKIFESRWKTVEKKLASATADPHVEVDRADSKRRKTPPVDCSDLSMECVRPTEIVKPTMTFEEKESFGNCLASLSEDPELPGYIIDLLQQCIDSSNTDHLGDGEIEIDIHALSDDILLELKKQVGKYLQERDNQPTKSEPSENEAVNVSGLSHSSTNPCKGGEPVEEDVDICGNASPILIEKDSQIKASKCGSPSSSSSDSGSSSSDSDSGSDSESESEKVGSPTKLVKGIKIPEQPAEQEKSDVISPIDANHTAADVELREQDSESKAAPEGENAKPDREVSPDKLLRAAVLRGRYADVIVKARGILSQGGDKHEELEKLQKEEKERLLAEGNAAMEARKAEAEAESKRKRDLEREKARQALQEMEKTVEINDNVYPKELEMLGTATTEHIVSSVDETSPEHSQDGMPSFLPGSGSMLEKLGLFMKVDEEEEEEEPNSIPSSKDAEEGEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1IH09</accession>
    <name>I1IH09_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100823633</name>
        <name evidence="4" type="ORF">BRADI_4g03215v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579228.2">
        <property type="nucleotide sequence ID" value="XM_003579180.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IH09"/>
    <dbReference type="FunCoup" id="I1IH09">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ86088">
        <property type="protein sequence ID" value="KQJ86088"/>
        <property type="gene ID" value="BRADI_4g03215v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823633"/>
    <dbReference type="Gramene" id="KQJ86088">
        <property type="protein sequence ID" value="KQJ86088"/>
        <property type="gene ID" value="BRADI_4g03215v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823633"/>
    <dbReference type="eggNOG" id="ENOG502RRNZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023151_0_1_1"/>
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    <dbReference type="OrthoDB" id="1939276at2759"/>
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        <property type="component" value="Chromosome 4"/>
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    <dbReference type="ExpressionAtlas" id="I1IH09">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055302">
        <property type="entry name" value="F-box_dom-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141:SF47">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="3">
        <location>
            <begin position="135"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="FBD" evidence="2">
        <location>
            <begin position="384"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ86088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="486" mass="54647" checksum="743462A568A61506" modified="2012-06-13" version="1">MDNSPSPKKRKLAASMIHEAPMSPEATEEAADLVARNQQLPPTAGADRISKLPDAILGDIISFLPTKEGARTEALASRWRGLWRSSAAPLNVDCGDLPCDDRVLDIFSSIVSNHPGPGRRFCVPSLVLGDRPDAVDAWLRSPALGNLQELEFWYRPYRRPEPLPHPPPSSMFCFSATLRVLTIGNCSLADETVQGLHFPLLQQLGIELVSVSECSLHRLIAGCPVLDCLLINHGYGFRCLRINSLTVRTVCVKNFRQVNDQLKELIVENAPCLERLLHLDFDYGLHVSVLSAPKLETLGCLTDGAYISKQDYLSRFEFGTTVIQGLRVDSLTTVVRTVKILAINMKTLSLDIVIELLRCFPCLEKLYIEAESMRVKNEWRLKHRSLVKCLDIRLKTIVFQSYQSIKSDVDFITFFVLNARVLESMTFQIGARDCNEEFIAEQRRKLQIENRASRGAQFHFTSGRCARSVWDIHHVSDLDLADPFLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1II20</accession>
    <name>I1II20_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1-acylglycerol-3-phosphate O-acyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100825849</name>
        <name evidence="10" type="ORF">BRADI_4g06330v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="5"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575968.1">
        <property type="nucleotide sequence ID" value="XM_003575920.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1II20"/>
    <dbReference type="STRING" id="15368.I1II20"/>
    <dbReference type="EnsemblPlants" id="KQJ86561">
        <property type="protein sequence ID" value="KQJ86561"/>
        <property type="gene ID" value="BRADI_4g06330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825849"/>
    <dbReference type="Gramene" id="KQJ86561">
        <property type="protein sequence ID" value="KQJ86561"/>
        <property type="gene ID" value="BRADI_4g06330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825849"/>
    <dbReference type="eggNOG" id="KOG1505">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041844_5_0_1"/>
    <dbReference type="OMA" id="LAGWMIC"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00613"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1II20">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF24">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 3, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="9">
        <location>
            <begin position="86"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ86561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="374" mass="42458" checksum="75A54787D1844FF0" modified="2012-06-13" version="1">MAIPLVLVVLPLGLLFLLSGLVVNAIQAVLFLLIRPFSKRLYRQINVFVAELLWLQLIWLVDWWAGVKVQLHADQETYESMGKEHALLISNHRSDIDWLVGWILAQRSGCLGSAIAIMKKSSKFLPVIGWSMWFAEYLFLERSWAKDEKTLKSGLQRLKDFPRSFWLALFVEGTRYTPAKLLAAQEYAVSQGLTAPRNVLIPRTKGFVSAVSIMRDFVPAIYDTTVIIPEDSPKPTMLRIFQGQSSVVHVRIKRHPMSDMPSSDEDVSRWCKDIFVAKDALLDRHIATGSFDEEIRPIGRPVKSLVVVLSWSCLLLYGAHRFLQWTQLLSTWKGVILFVAGLAMVTAVMHVFIMFSQSERSSSAKAARDRVKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1III8</accession>
    <name>I1III8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100830658</name>
        <name evidence="3" type="ORF">BRADI_4g07730v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576342.1">
        <property type="nucleotide sequence ID" value="XM_003576294.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1III8"/>
    <dbReference type="STRING" id="15368.I1III8"/>
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        <property type="entry name" value="DUF4408"/>
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        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35762">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
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        <location>
            <begin position="46"/>
            <end position="64"/>
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        <location>
            <begin position="24"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ86791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="201" mass="22074" checksum="9B5DB87E020D36D9" modified="2012-06-13" version="1">MDIQSCVKASLLALGLLSLATSLPHLYSCLRTFLVVSLPSVASAVTAPKCLFVFSNIIVVFLVGECKLSHGGKSPDIGDDDVLNDQRQEQEEEFHQEELVMETALGALVVTGASEQEEEGNMAVVQEEEGSLVVLQEGHHVVQMDQDEGSIMEVRVGFTLEEEEESSGLPAADELNRRVEDFIARFNMERQLEARMLVCCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1INV4</accession>
    <name>I1INV4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DEAD/DEAH-box helicase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100844213</name>
        <name evidence="7" type="ORF">BRADI_4g26640v3</name>
    </gene>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003577844.4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="15368.I1INV4"/>
    <dbReference type="EnsemblPlants" id="KQJ89597">
        <property type="protein sequence ID" value="KQJ89597"/>
        <property type="gene ID" value="BRADI_4g26640v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844213"/>
    <dbReference type="Gramene" id="KQJ89597">
        <property type="protein sequence ID" value="KQJ89597"/>
        <property type="gene ID" value="BRADI_4g26640v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844213"/>
    <dbReference type="eggNOG" id="KOG0339">
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    </dbReference>
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        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:helicase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        </location>
    </feature>
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    </feature>
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            <begin position="10"/>
            <end position="20"/>
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    </feature>
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        <location>
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            <end position="46"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF00270"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ89597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="521" mass="56291" checksum="72F923AE9784CBB7" modified="2012-06-13" version="1">MAKGDDALARKRGKVRRKRMRSSENAVSTRVAAIIASKRRRKTGKRRGCEGMCFSLPSPEDPFNDRHGKKKKDGASPNPDAAISDDEPPKKKLKPPPKEKAAAAAGGGRGRRKNPVAPELEAGEEKYDRPSKFLVVCLNAIRDAAAAGVDGDLVHEDASWGLEFWKCCSASPPSDVLHAGEGCAPPGLAAWLISAACDIVARKEKLGVVVPCPFLLYIVPSQERALEVRLICKPLKSLGIHSVSLHPGASVEHQISGLKSCEPEFLISTPERLLELVSRKAIDISNVSMMAIDGLKTFMDLNISDKLYSIKGAISSDAQITIFNDTCDQSAVPVVKSLLRGRITKLSVNDCSLNHRSAFIAQHIHFCPSEEKKATQVKEILEHVLDSSASKTAKVLLVAADDHEAQNLASSLKLQTCAVIDDTHRGTFTICSSKGLINVLVKGQDSLAKSDIEEFEIVVVSDLPRSIDDYKEIISGMSRHAVSGEVHVIFSNDDAALADPLADVLSKCGQMVPKFVRKLCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITY2</accession>
    <name>I1ITY2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g41250v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITY2"/>
    <dbReference type="FunCoup" id="I1ITY2">
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    </dbReference>
    <dbReference type="STRING" id="15368.I1ITY2"/>
    <dbReference type="EnsemblPlants" id="KQJ92022">
        <property type="protein sequence ID" value="KQJ92022"/>
        <property type="gene ID" value="BRADI_4g41250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92022">
        <property type="protein sequence ID" value="KQJ92022"/>
        <property type="gene ID" value="BRADI_4g41250v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R7BX">
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    <dbReference type="OrthoDB" id="627373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="61" mass="6359" checksum="A07F6898FB207155" modified="2012-06-13" version="1">MDAAKKRDVTGAGAAAAKEEENRKRGHGSTALFVAVDYAFLLAFAGFLSYLVGSQLLPSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVC1</accession>
    <name>I1IVC1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100843515</name>
        <name evidence="3" type="ORF">BRADI_4g45360v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579070.1">
        <property type="nucleotide sequence ID" value="XM_003579022.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVC1"/>
    <dbReference type="FunCoup" id="I1IVC1">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IVC1"/>
    <dbReference type="EnsemblPlants" id="KQJ92705">
        <property type="protein sequence ID" value="KQJ92705"/>
        <property type="gene ID" value="BRADI_4g45360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843515"/>
    <dbReference type="Gramene" id="KQJ92705">
        <property type="protein sequence ID" value="KQJ92705"/>
        <property type="gene ID" value="BRADI_4g45360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843515"/>
    <dbReference type="eggNOG" id="KOG1455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026209_0_1_1"/>
    <dbReference type="OMA" id="YPAMWHA"/>
    <dbReference type="OrthoDB" id="2498029at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000036">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="41"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="337" mass="37294" checksum="1E9590B86F59C50D" modified="2012-06-13" version="1">MTSKYTGVVDEGNYSYEEEMVNNARGMKLFTCRWLPPKSQPVKALVFICHGYAVECSVTMRGTGVRLAQAGYAVYGVDYEGHGKSEGLQGYVPSFDLLVNDCDAFFAAAVASTPNTDLPRFLLGESMGGAVALLLHRARPSYWSGAVLVAPMCKIADEMKPHPVVVSVLRAMTSIIPTWRIVPTADVIDAAYRVQEKRDEIRANPYCYNAKPRLKTAYELLRISLHVENNILKKVSLPFLIVHGGDDKVTDPSVSDLLYRSAVSQDKKLNLYPAMWHALTSGETVDNINVVFKDIIAWLDQRSGASPAPTTSSSEMEQKSKHDHDQHQQDLNVVAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IWG0</accession>
    <name>I1IWG0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100841305</name>
        <name evidence="17" type="ORF">BRADI_5g03930v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581418.1">
        <property type="nucleotide sequence ID" value="XM_003581370.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IWG0"/>
    <dbReference type="STRING" id="15368.I1IWG0"/>
    <dbReference type="EnsemblPlants" id="KQJ81935">
        <property type="protein sequence ID" value="KQJ81935"/>
        <property type="gene ID" value="BRADI_5g03930v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841305"/>
    <dbReference type="Gramene" id="KQJ81935">
        <property type="protein sequence ID" value="KQJ81935"/>
        <property type="gene ID" value="BRADI_5g03930v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841305"/>
    <dbReference type="eggNOG" id="ENOG502QWDY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_35_3_1"/>
    <dbReference type="OMA" id="TSTHEFH"/>
    <dbReference type="OrthoDB" id="617804at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IWG0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000129">
        <property type="entry name" value="cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000142">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF76">
        <property type="entry name" value="OS10G0136400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095598" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="20"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="16">
        <location>
            <begin position="18"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="16">
        <location>
            <begin position="149"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="364"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ81935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="682" mass="73838" checksum="212903BE04967271" modified="2012-06-13" version="1">MAGVVLLLSFLLMPLSASATGKVCGKGGNYTANSTYSSNLSLLAAALPLNASSSPTLFATATAGQSPDAVHALALCRGDTAADSNATASCGGCIADSFRYAQELCPNARAAAVYFEYNDSDSRPGCLLGFSPDAAFLGLTPAAIAAADGSTLFQFWNGQSIPGDAAAVAADVRELLNATARDAAAAAVRRFATVTMDSGSSDIPTLFSLAQCTPDLPAGDCLACLQRLVAMVNDTTAVRQGGRILVLRCNIRFEAFMFYNGESMRRIRPSSGVTPAPPATGPTGKRNGVRPWVIAISVAAPVALVAFGFILYYRRLRRRYTKGKVRSHGKRARKLKGGDELLWEMEAEFSVFDFQQILEATCNFSEENILGEGGFGPVYKGQFPDGMEIAVKRLASHSGQGFIEFKNEVQLIAKLQHRNLVRLLGCCSQGEEKILVYEYLPNKSLDFFIFDEDKKALMDWNKCLAITEGIAEGLLYLHKHSRLCVIHRDLKPSNILLDSKMNPKISDFGLAKIFSSNATDEGNTTRRVVGTYGYMAPEYASEGLFSVKSDVFSFGVLILEILSGKRNSGSNQCGDFINILGYAWQLWEEGRWIEIVDASLNPKSHSEEIMRCINIALLCVQENAADRPTMLDVVAMLSSKTMILRETKHPAYFNLRVGNEEASTGTQSCSVNDLTISVTTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1J2I3</accession>
    <name>I1J2I3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit epsilon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100829550</name>
        <name evidence="14" type="ORF">BRADI_5g23800v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the COPE family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579377.1">
        <property type="nucleotide sequence ID" value="XM_003579329.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J2I3"/>
    <dbReference type="STRING" id="15368.I1J2I3"/>
    <dbReference type="EnsemblPlants" id="KQJ84927">
        <property type="protein sequence ID" value="KQJ84927"/>
        <property type="gene ID" value="BRADI_5g23800v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829550"/>
    <dbReference type="Gramene" id="KQJ84927">
        <property type="protein sequence ID" value="KQJ84927"/>
        <property type="gene ID" value="BRADI_5g23800v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829550"/>
    <dbReference type="eggNOG" id="KOG3081">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049363_0_0_1"/>
    <dbReference type="OMA" id="LAMNVQI"/>
    <dbReference type="OrthoDB" id="310217at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000140">
        <property type="entry name" value="Coatomer subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006822">
        <property type="entry name" value="Coatomer_esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10805">
        <property type="entry name" value="COATOMER SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10805:SF2">
        <property type="entry name" value="COATOMER SUBUNIT EPSILON-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04733">
        <property type="entry name" value="Coatomer_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016478">
        <property type="entry name" value="Coatomer_esu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR016478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ84927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="293" mass="32057" checksum="BC73DA2D944752AA" modified="2012-06-13" version="1">MAAAPDGLFGLRNSFHVGAYQAAITGSQSAPSHALSPDEVVERDALLYRSYIALGSHQLVIDEIGPRAATPLQAVKLLATYLISSGNKESVISKLKELLGDAAVGSNPILRLVAGTVFMHERDYAEALKHTNSGGSMELLALNVQIYLLMNRPDHAEKQLRVMQQLDEDHTLTQLANAWVNLVMGGSKIREAHLIFQDFSEKYPATCMILRGKAQCLMHMGKFEEAEGLLLESLNKDAKDAETLANLIVCSLSLGKPASRYLNQLKLAHPEHMLVKRMSSAGDSFDRACQEMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ECI8</accession>
    <name>A0A0Q3ECI8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Peroxidase</fullName>
            <ecNumber evidence="19">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100832395</name>
        <name evidence="22" type="ORF">BRADI_5g27200v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in defense response to powdery meldew fungus.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="19"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85478.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240654.1">
        <property type="nucleotide sequence ID" value="XM_010242352.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ECI8"/>
    <dbReference type="STRING" id="15368.A0A0Q3ECI8"/>
    <dbReference type="EnsemblPlants" id="KQJ85478">
        <property type="protein sequence ID" value="KQJ85478"/>
        <property type="gene ID" value="BRADI_5g27200v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832395"/>
    <dbReference type="Gramene" id="KQJ85478">
        <property type="protein sequence ID" value="KQJ85478"/>
        <property type="gene ID" value="BRADI_5g27200v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832395"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3ECI8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000009">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF51">
        <property type="entry name" value="PEROXIDASE 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 19" id="KW-0349">Heme</keyword>
    <keyword evidence="12 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="8 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="5 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043073406" description="Peroxidase" evidence="19">
        <location>
            <begin position="23"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="21">
        <location>
            <begin position="24"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="293"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="34"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="67"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="118"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KQJ85478.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="326" mass="34549" checksum="1FF8DBDB3CA12A0F" modified="2018-03-28" version="2">MTMATAMVAAVLLLLTISPAAAAALSPHFHADSCPELESIVRTSVEAALEEEIALAAGLLRVFFHDCFPQGCDGSVFLTGRNSEQSLGPNLTLQPRALQLVDAIRAKAHAACGPTVSCADISALATRDAVVFSGGPNYTVPLGNLDSLTPASAKAVMTLPSPITSSVAALFEAFRTRGLVELTDLVALSGVHTVGRAGCRFFADRSAKQDDTFSKKLAANCSVEPDRLQNLDVITPDVFDNGYFKALMFNQGVFTSDMALVKDKDTAPIVKRFAGSKDKFFKQFVKSMEKLVNAPRPDGNVGEIRRSCSSPNGDDGEKEDFAVASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ED69</accession>
    <name>A0A0Q3ED69_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_4g00620v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85615.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ED69"/>
    <dbReference type="EnsemblPlants" id="KQJ85615">
        <property type="protein sequence ID" value="KQJ85615"/>
        <property type="gene ID" value="BRADI_4g00620v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ85615">
        <property type="protein sequence ID" value="KQJ85615"/>
        <property type="gene ID" value="BRADI_4g00620v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3ED69"/>
    <dbReference type="OrthoDB" id="687885at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3ED69">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1062">
        <property type="entry name" value="OS11G0579400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="693"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="808"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="151"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1112"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1126"/>
            <end position="1140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ85615.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1156" mass="132067" checksum="B230061BAE8B9560" modified="2018-03-28" version="2">MAELATGAVSTLLGVISNEAQRLGRVRGDVQFIQEEMESMSSFLANLSRWNIRDQQDDGGGGDDDDQVRTWMNQVRILANDCNTCIDLYLYRGDPELHRSKAGLRRYLYLWWAPWLMRKTLAQHRAAGQLRKLKERAREIGERRLRYGVEVKKSSSSGSGLTKEDGHGEEEDDDDDDDGARRSLKVALYMMNMDEEDYLKWRLDDQWIRQVVLQGTSGLKQVFLPSIVFLVPGTEDASLLADQAMVVAERALTIPLVPLDARGAPASEEAHLEHHGEEEDESASVHIYKIPMKYRQNVALVDVSEMHYHLLPLRPEDLLYYILHELLRHIPKPQSSKEEDQQQATAPPPTPFSVYRSKREVFREIKHEIQERNEVNKKIEGLKTEICQALKSKEGRKQDKKDVKCEELSLHKLFCLLIESTVAATEQDRASKATRKLEAPYNYIIKQTAMKLKEEIEGGTNKRAPEYEHILCNVFPKPATGATVDDQIKEIEETTRKNMEEIEKKITKQLKIKGIVRMIQDCFEGDKRIPVILRTDGNCVSKWKETRNTLSLLGCAPIAGAIIVATKTTQLQDFKEEDLCYPPLELIEYSPVGRFLDTVLQVTRQHMREGSRQILRKIFYECEDADEFCMKIFAHAMYDKPKRGSQELRKLHMSIMHDHDSQKSLPSVAKKMFKFSYKDLPKQYRSCLLYLAIFPQGEEIKRSTLIERWVAEGLITTKKWSWSSSVAEAENCFNTLIARCLVCPAGNGIGVAGRFKSCKVDEPVYGFITKIAMKQRILDTRLSGGLARHFSIFSDVRLRNSEKVEDFLKSIPKSSQFSKLKVLDLEGVACLAGKHHYYLRAICSKILMLKYLSLRRTDVTELPREINNLLELEVLDIRQTTIPAYQTRKVLILKLKRLLAGHVAGFSSVQIPERIEKMEGMEVLSNVELKIERDLDDIASLCMLKKLDERTLPGLQQCSSELPENKFPKVLESLSIIGSTTQEYDLHLLTVFAKDANQLAKVTLRRTLLSQKGLEVLAKLPKLSCVRLRYIKYTHTELTFIKGEFQNLAGFVIEGPGITGIKFENGAANHLEKMVCLFTNIESLSGVDNLPSIKKIEFNGETVPKEVEEAMGRQTRRKLYTCARTNKPESESKQQERPDDNTSSSWKKVKCLGWRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EK38</accession>
    <name>A0A0Q3EK38_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100839852</name>
        <name evidence="3" type="ORF">BRADI_4g07580v3</name>
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    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003576251.4"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3EK38"/>
    <dbReference type="EnsemblPlants" id="KQJ86764">
        <property type="protein sequence ID" value="KQJ86764"/>
        <property type="gene ID" value="BRADI_4g07580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839852"/>
    <dbReference type="Gramene" id="KQJ86764">
        <property type="protein sequence ID" value="KQJ86764"/>
        <property type="gene ID" value="BRADI_4g07580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839852"/>
    <dbReference type="OrthoDB" id="1588785at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS12G0472800 PROTEIN"/>
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
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        <location>
            <begin position="18"/>
            <end position="450"/>
        </location>
    </feature>
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            <end position="450"/>
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    </feature>
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            <end position="450"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="450" mass="48225" checksum="8679C8C0B6B17329" modified="2016-01-20" version="1">MPLHMRLFHVSWRLASSALPVPFSSSACPYSNCALIALDLQVPIISQPLLANALAPTTSYAGYKTGRSGVSSKMAKPMSFLVLQVLLVPVLLFLLPGAECQSSHGGGANLTVTGTVFCDACSRSSFSNHSYFLPGVRVRIDCTFGANSPSKEEIKITAEKTTNSYGAYQLDIPAIDGVGCTTAAAAVSFCRAAVLDNPSALCNVPALTTTVGHISFSTQAQEPNSNANVCLYNLNSLYYRPRPNKGPGLCDDDGGGPLQPAALNDSLFYCPRWPWPPIPFCTPRPWFPPIPFFTPPPPAFPFPLPPFPPFPFFTPPSPPPPAFPFNLPPWPWTPPAPAPAPPSFPFPRLPPIFSAPSPPPPPPPPAFPFPNLPPFPHLPPFAFPPLPSFYSPPPPPPPPPPPPPPPSFHWPFPPLPFFPPTSPSAPSPPPLKRSRKDPTTWSSSKTTPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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            <strain evidence="2">cv. Bd21</strain>
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    </dbReference>
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    <dbReference type="InParanoid" id="A0A0Q3ENB4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ87756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="78" mass="8872" checksum="42998F6CDDE2B16C" modified="2016-01-20" version="1">MFLLAIQSDQTLYVPLVLLQPQREIRTSKNKGGSKTHPCACLMACLSWKSNSMERPVFWIGWKSCQCSTGFWFVCGNI</sequence>
</entry>
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    <accession>A0A0Q3ET82</accession>
    <name>A0A0Q3ET82_BRADI</name>
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        </recommendedName>
    </protein>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
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    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576669.1">
        <property type="nucleotide sequence ID" value="XM_003576621.4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3ET82"/>
    <dbReference type="EnsemblPlants" id="KQJ90726">
        <property type="protein sequence ID" value="KQJ90726"/>
        <property type="gene ID" value="BRADI_4g33520v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826898"/>
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        <property type="protein sequence ID" value="KQJ90726"/>
        <property type="gene ID" value="BRADI_4g33520v3"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000020">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF424">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UPL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="55"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="9">
        <location>
            <begin position="514"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="13"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ90726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="852" mass="96206" checksum="32C527CDBCCF4D5D" modified="2016-01-20" version="1">MSPPAALACYRRNAKRRRDAPDHALPSSKQPVMTTHSAASSSGAGGSLYASPRSVHFFVRATDSKTIAMHAAWDDTVGAVLDHLAGCGYGRDLRLLYAGRQLLPEATIASLCLPPDSTLHLAARLRSTPHPEAWQLASHIAATAAAAEAAAASTPTHSLDWLVKEYIICASSRRKRGDRDDPVDTLSTEHRAEEYLDIFLQAGAAVALVRLYLSNSSFFRSFAERAIKCFLSMDPVSPIPAAIKPVTAPVLLEFCRLLSVTAGSKDSLYKSCRYTLASVLCEPLSPLTSSKSPARVIEQVLPFAREVVEVVLSGLTSESMMVTRADLEEFSNFFKVLRHQVRRWMPDGPMRKNLYNREHEHSDTWVWELHEMSMNLLRSVDECLKRLEMDLSTLSSESRGVIESQPMWANRLHILAMLTELDFISAIFEDVAHNLRFVLLAHKAPLNALVRSSKRNEHLHWLVKHKDLLCFEARRNLVLMLFSEGKDDYGELHEMLIDRSHLLDESFEYITQARPSELHSGLFMEFKNEEATGPGVLREWFCMVCQALFSPQQVLFSPCPNDQRRFYLNGTSVVDPLHLKYFIFSGRIIGLAVMHKVQVGIVLDRTLFLHLAGRSITLEDIAAADPVTYASCKRILEMGATDIDELTLTFSRDIHTLGSRRTIELCAGGQDISVNIRNREHYIDLLIKNIFVDSISAQLANFAKGFSEILASPDLRKVFFGFLDLEDFDRILGGSNSTINLKDWRSHTQYNGYKEKDRHINWFWKVVESMTIEQQRQLLFFWTSVKYLPSDGFGGLSSKLYIYKTLESADHLPSSHTCFYRLCLPPYPSLKVMQNQLHKITQEHVSCSFGTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FJR7</accession>
    <name>A0A0Q3FJR7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100835858</name>
        <name evidence="9" type="ORF">BRADI_3g36610v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572226.1">
        <property type="nucleotide sequence ID" value="XM_003572178.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FJR7"/>
    <dbReference type="STRING" id="15368.A0A0Q3FJR7"/>
    <dbReference type="EnsemblPlants" id="KQJ98388">
        <property type="protein sequence ID" value="KQJ98388"/>
        <property type="gene ID" value="BRADI_3g36610v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835858"/>
    <dbReference type="Gramene" id="KQJ98388">
        <property type="protein sequence ID" value="KQJ98388"/>
        <property type="gene ID" value="BRADI_3g36610v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835858"/>
    <dbReference type="OrthoDB" id="71302at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FJR7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11455">
        <property type="entry name" value="bHLH_AtAIG1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000070">
        <property type="entry name" value="transcription factor bHLH30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045847">
        <property type="entry name" value="AIG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45844:SF11">
        <property type="entry name" value="OS08G0432800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45844">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="133"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="8">
        <location>
            <begin position="234"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="282"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ98388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="38504" checksum="2C060D3B5D9C5EDA" modified="2016-01-20" version="1">MWEGAGAVHGSHEALLLQATGGDRSIDHGQYSSGLLPWQLGPSSAGPSYMIPSAPFGVPESGGSFGDGFADGGGLQLQGQGQHQYGGLFGLEPPTELHGPSSSRMMVSGLLGSLQAELGRMTAKEIMDAKALAASRSHSEAERRRRQRINSHLARLRSLLPNTTKTDKASLLAEVIEHVKELKRQTSAIMAVSSASGEDHAAAPAVQRQLLLPTEADELEVDAAAGEDGRLVVRASLCCEDRPGLIPDVARALAALRLRARRAEIATLGGRVRNVLLITAADEDEEEEGDGGREEEDEDERAGAAGSHRRRHELVASIQEALRGVMDRKGAGASSDTNTTSSSGGGGGGGSSIKRQRVNGAREQGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G4N6</accession>
    <name>A0A0Q3G4N6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g25556v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06296.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G4N6"/>
    <dbReference type="EnsemblPlants" id="KQK06296">
        <property type="protein sequence ID" value="KQK06296"/>
        <property type="gene ID" value="BRADI_2g25556v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06296">
        <property type="protein sequence ID" value="KQK06296"/>
        <property type="gene ID" value="BRADI_2g25556v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3G4N6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06296.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="138" mass="14948" checksum="E92CB227CA41BC2C" modified="2018-03-28" version="2" fragment="single">SPPHTLSPPASFFSSSRELPPPSPRRGRPPPAPPCRRHRLRPPDPAAGARIRRRTAGLGRPRATAPLPCPPPHDATSPARPHATGRRRPAHDRPPSLPAPERPAAARPRTTRADAGLLPQLPPHARSFCVLVQFLDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GPM7</accession>
    <name>A0A0Q3GPM7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g07043v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <source>
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    </evidence>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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    </evidence>
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        <source>
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</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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                <person name="Harmon-Smith M."/>
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                <person name="Grimwood J."/>
                <person name="Bruce D."/>
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                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
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                <person name="Vogel J.P."/>
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                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 11">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
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            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
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        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 4"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="6 9 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="131"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="303"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00019371"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ85707.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="443" mass="47449" checksum="4B45D78F140EC141" modified="2018-03-28" version="1">MATNAVQIQRTPFPSSSSSPCRANARSTSTGQQPPGGPTVIFTVHRRPGLAGRPIPLLSPHQPTKPVPCIKHRQLHSPFPSISSRSRHFFSFFFFFFLHHRREDSLRCRIAKLVEGGGMGEAATPAIAKDVTELIGNTPLVYLNKVTDGCVGRVAAKLESMEPCSSVKDRIAFSMITDAENSGFIVPGKSVLIEPTSGNTGIGLAFMAAAKGYRLVLIMPASMSMERRIILKAFGAELILTDPLLGMKGAIQKAEELAAKTPNSYILQQFENPANPKIHYETTGPEIWRSTGGKIDGLISGIGTGGTITGTGRYLREQNPNIKLYGVEPVESAVLNGKKPGPHKIQGIGAGFIPGVLDVDLIDETVEVSSDESIEMAKALALKEGLLVGISSGAAAAAAIKIAQRPENEGKLFVVVFPSFGERYLSSVLFQSIKKEAESMVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IEY6</accession>
    <name>A0A0Q3IEY6_BRADI</name>
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        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100844898</name>
        <name evidence="8" type="ORF">BRADI_3g41547v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572462.1">
        <property type="nucleotide sequence ID" value="XM_003572414.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IEY6"/>
    <dbReference type="STRING" id="15368.A0A0Q3IEY6"/>
    <dbReference type="EnsemblPlants" id="KQJ99171">
        <property type="protein sequence ID" value="KQJ99171"/>
        <property type="gene ID" value="BRADI_3g41547v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844898"/>
    <dbReference type="Gramene" id="KQJ99171">
        <property type="protein sequence ID" value="KQJ99171"/>
        <property type="gene ID" value="BRADI_3g41547v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844898"/>
    <dbReference type="OrthoDB" id="775582at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IEY6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
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        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
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        <property type="entry name" value="AP2/ERF_dom"/>
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        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF167">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF112"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
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    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="73"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ99171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="147" mass="16255" checksum="653355A992E683AB" modified="2016-01-20" version="1">MNSHQRNLVKIKDYSIVAPDTKNPLSDPYPSNLMFQTSVGPTGLSVGYMRPAPAPASDVQQRAEEQQKAMRRRNRAVWRRLWGKYSAEIWDPVNARRVWLGIFTTAEDTFSAYDAAAIRFKGPGKAKLAATNDAVATSGSPGDQELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JI65</accession>
    <name>A0A0Q3JI65_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100842797</name>
        <name evidence="5" type="ORF">BRADI_1g00820v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK11988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753371.1">
        <property type="nucleotide sequence ID" value="XM_014897885.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JI65"/>
    <dbReference type="FunCoup" id="A0A0Q3JI65">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3JI65"/>
    <dbReference type="EnsemblPlants" id="KQK11988">
        <property type="protein sequence ID" value="KQK11988"/>
        <property type="gene ID" value="BRADI_1g00820v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842797"/>
    <dbReference type="Gramene" id="KQK11988">
        <property type="protein sequence ID" value="KQK11988"/>
        <property type="gene ID" value="BRADI_1g00820v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842797"/>
    <dbReference type="OrthoDB" id="340227at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JI65">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07323">
        <property type="entry name" value="LAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF108">
        <property type="entry name" value="LA DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="4">
        <location>
            <begin position="316"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="293"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="408"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="410"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="453"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK11988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="472" mass="49460" checksum="638E5A300C5DDEFE" modified="2016-01-20" version="1">MEPAPGSPDPSAHKDQHQNNSPAPAKRSAWKQPASSSNGVHLVDAPPPGVMDADHWPALADAANSKAKTAPAPAPAATPPSVDSPKPAPTENPSPAAVAAPSSAVANPSNSPRHGPGPHHGRHRPGRRGGAGGNGDHSPRDHHDRGNNSGWDHGSGSGGGRGGQRTHHHNNGGAGAGRRGGGNAGGGPSGGVPHHGGFGGRRRGGFEGFYRGPPMGIGPYMRGVPPPPPPIAVAPPYMGPPPPPMRAFAGPMMFHEMPSPVSPVPPLYYVGPPPPPEALRGMAFQPTMVGPPAYPYFQPPAESEPEPEPEPDPEPEAETEDEQTKLMRQIEFYFSKDNLCTDVWFRQQMDAEGWVDIPLIATFKKVRAFNTTLQYIKEAIQSSSMLEMQGDKVRRENDWKKWLIPRADIPSSSAAGPSPNVSNLTAHLGGVSLHESAGPSSKGDENRPEVVPNGSTSSNNQAAVVEDGAGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JXU4</accession>
    <name>A0A0Q3JXU4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g31109v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16860.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JXU4"/>
    <dbReference type="EnsemblPlants" id="KQK16860">
        <property type="protein sequence ID" value="KQK16860"/>
        <property type="gene ID" value="BRADI_1g31109v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16860">
        <property type="protein sequence ID" value="KQK16860"/>
        <property type="gene ID" value="BRADI_1g31109v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JXU4"/>
    <dbReference type="OrthoDB" id="695318at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46250">
        <property type="entry name" value="MYB/SANT-LIKE DNA-BINDING DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46250:SF4">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK16860.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="241" mass="27203" checksum="3A4D00F202BF17AC" modified="2018-03-28" version="2">MNDSCWKVDTGHKSGYLTYVEKKMAIKLPYADLKADPHIKSKVKILKKQLSYILEIMQNGSGFGCDDEKKMVTGDRETYMGWAKDVAEDEPTKDGEHTIELGEEDVSEAHDNANNTSGGHKNGRKRTYADLDTLGMGFVNVSNSFAKFLEAEQQNTNTMNGIHKALQRESDVHDKASENRDKFLEVLQDLPGLTDKEVVMAIRVIGPDAGRIDFFLKMRAQFKVEFVRQELEAAKKKQLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JY20</accession>
    <name>A0A0Q3JY20_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BRADI_1g31494v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JY20"/>
    <dbReference type="EnsemblPlants" id="KQK16932">
        <property type="protein sequence ID" value="KQK16932"/>
        <property type="gene ID" value="BRADI_1g31494v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16932">
        <property type="protein sequence ID" value="KQK16932"/>
        <property type="gene ID" value="BRADI_1g31494v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JY20"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF13-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768:SF34">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="113"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="170"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="170"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK16932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="214" mass="22365" checksum="665E11B832D92903" modified="2016-01-20" version="1" fragment="single">MGGLGAVYMMTTLLIIFGIIGFWFGWCGRRFAGGHSRSRGRAAGAGAGARAAEPVPAEAEAGPGPAQGSGLLLFHVLWRAYQTSLQMMRPAAGLSDAAIAALPLTKLARAAECAVCLGEVAAGGVARLLPLCRHTFHVECVDTWLRSRANCPVCRQTVNEVNEMPRNDVQDAAQPAAQAQDQAQAEGPVVLPQADGRAPLAQARDSGEGHISLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K5W7</accession>
    <name>A0A0Q3K5W7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g24954v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK06177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K5W7"/>
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        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3K5W7"/>
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        <property type="protein sequence ID" value="KQK06177"/>
        <property type="gene ID" value="BRADI_2g24954v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06177">
        <property type="protein sequence ID" value="KQK06177"/>
        <property type="gene ID" value="BRADI_2g24954v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K5W7"/>
    <dbReference type="OrthoDB" id="298344at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34194">
        <property type="entry name" value="F14J8.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34194:SF25">
        <property type="entry name" value="OS05G0423200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK06177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="349" mass="39299" checksum="A11D613E24AF1B76" modified="2016-01-20" version="1">MSNRSSRDKNKVSDTLYGEDVDPAYKMFLDHLSKDGDSYVLHVPNGDHGMPVTVRYEVANDKDGTDTPNISPCISQGGVNIRRPGVTSAGAANVSVGQSITPRTSSLESKTSEIDESYAKFLSLTKMVDGFMVTEIEPGVTIVYEQEEETPAAYGELKTVSDTPVRVYSRRTVSSRKERAPLTTALENMEEEDEVRTDEDRLEQTDNRHNVISKESHYPEACEDDQGAPLGLPSGVTSTFDEQLDSFLSRPYDRNEFQELLRKATFRKPVTRQRHLRNASKFYATGELGLSYLDEYPDLATQIDSADCDERRLNLLRKFFFWLQNLTQEGAYMPWIPKAPVSNPINVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3L3K3</accession>
    <accession>A0A0Q3EHF5</accession>
    <name>A0A0Q3L3K3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100827806</name>
        <name evidence="16" type="ORF">BRADI_4g09530v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87166.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575641.2">
        <property type="nucleotide sequence ID" value="XM_003575593.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310908.1">
        <property type="nucleotide sequence ID" value="XM_024455140.1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3L3K3"/>
    <dbReference type="EnsemblPlants" id="KQJ87165">
        <property type="protein sequence ID" value="KQJ87165"/>
        <property type="gene ID" value="BRADI_4g09530v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ87166">
        <property type="protein sequence ID" value="KQJ87166"/>
        <property type="gene ID" value="BRADI_4g09530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827806"/>
    <dbReference type="Gramene" id="KQJ87165">
        <property type="protein sequence ID" value="KQJ87165"/>
        <property type="gene ID" value="BRADI_4g09530v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87166">
        <property type="protein sequence ID" value="KQJ87166"/>
        <property type="gene ID" value="BRADI_4g09530v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827806"/>
    <dbReference type="OrthoDB" id="2668416at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3L3K3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF36">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0611">Plant defense</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="13">
        <location>
            <begin position="11"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="11">
        <location>
            <begin position="181"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="14">
        <location>
            <begin position="438"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="567"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="12">
        <location>
            <begin position="1190"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="12">
        <location>
            <begin position="1543"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1698"/>
            <end position="1765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1707"/>
            <end position="1725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1734"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1755"/>
            <end position="1765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQJ87165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1765" mass="200503" checksum="B917A72E829D537C" modified="2016-01-20" version="1">MAMVLDAFASYVGDLLKQVVEDEVGMLLGVTGDIENMGIKLGELKKLLTDADRRNITDESVQEWVAELKHAMYEATNILDLCHLKAMEHGPSTVNAGCFNPLLFCLRNPLHAHNIGSRIKTLNQKLDKIKKQSTEFSFILSSYEDPGSRVQAEKANHTRESTGQLDVSGVVGEKIEEDTRELVVRILQKRNEIGNNIMVIAIVGVGGIGKTTLAQKVFNDDSIQGEFDKRIWLSVNQNFDKTELLKTAITLAGGDHHGEKVLAVLQPILTTALTGKKFLLVLDDLWSHGAWEGVLKIPLVNTAVSGSRVLITTRHEGVARGMTATWPHHHIDTLSSDDAWSLLKKQVLSSGTDEYHVNTLKDVGLKIIKICGGLPLAIKVMGGLLRQREMHRRDWEQVLDNSDWSTTKMPEDLNNAVYLSYQDMPPYLKQCFLFYALLPKSTRFDVLHVVGMWISEGFIHGNHSDLEETGRNYYKELISRNLIEPDNKSYFEQWFCSMHDVVRSFGQYIARKEALIAHNGEIDTLTKLNSQKFLRLSIETSQLQSGELHWKSLQEQESVRTLISTIPIEMKPGDSLVTFSSLRTLYIESADVALLESLHKLKHMRYLTLVNTSISALPENIGKMKLLQFIDLSECNNLVDLPNSIVKLSQLRYLNLPSNGSIISRGFCGLTNMRILGGFPALVDGDWCSLDELGPLSHLRFLRLEQLENVSTASSAANVRLGEKIRLTKLLLGCTSKLGYDGLVRREDVSAEEQQRIEMVFNQLCPPSSVEYINISGYFGQQHPSWMMSMPTVPLNNLKFLLLCDVACCNQLPSGLCQLPCLQELQVRRAPCIKRVGTEFLHSSQPVAAAFPRLNKMVLKRMVEWEEWEWEEQVQAMPCLEELLLKRCKLRLVPPGLASQARALRKLSIKHVKQLTYLENCMFVVDLTVDRCPDLERITDLPKLQKLTIIDCPKLKVLNGVPQLDRVVLEDYTMEELPEYVREIKPRHLQVFCRLWLLASVATGQSGIEWDKFSHVEYVKSYARDGDNRRKWYVLYTRGNCKLESNISRSTVFQETLSSSMIETQRFEHLYKMKITTFDYVCSLVRVPFLEDMMARDHRFADGRVLCLQDRVAVALRMLNSGEPPETVGSSLGMNKSIVLLITKSFVDAMWEKAMHHLDWPGSNRIENIKYKFDKIHGLPNCCGVVHTDHITFESQNSDHEVNAGMLMQAVVDTDMRFTNIWLGSSSNMNQSSILHDSVLFKHCEKDTWLNGSKLNLSDGRQVGEYIIGDAGFPLLPWLLTSFQENDLSDYQVEFNRRHSQAMTITLTKALAKLKDTWKFLHGGVWRPENQFEPMWVIYACCMLHNIVIDKECGTGMGSYQKVNYSQQVHQLVDEDPVMVRDVLSQHLTSKPLEPGGTLSIFEDAQTLESVLKMPRRTFNYVCGLLKESSLEIMNDYFFFDMRLFSLEERVAIALIMLNSGDPPATVGSFIGVNESTVPLVTKSFVDAMLERAQHHLRWPQSDEMEKMKSMFDEIHGLPNCCGVLHTTHVTSASRSWDHFDKDSFVLQGVIAPDMRFTSIWVAPRPANTSQSSFLHDSNLFEYCEKGAWLNGSKLKVASEEVGEYVIGDVGYPLLPWLLTPYYQLQNDLSDIPYQVEFNSRHSAVKNIALKVLARLEGTWKSMHGEWRPDTPREMSRAIHACCILHNIVIDIEEDAGMPSDQEEDDSKQKRQLEDEDAVRARDVLSEYMTSRSSESRVDEVDQEVQHRKQLHPVQGEKGKQEAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LCD9</accession>
    <name>A0A0Q3LCD9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100821849</name>
        <name evidence="11" type="ORF">BRADI_4g29947v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576516.1">
        <property type="nucleotide sequence ID" value="XM_003576468.4"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3LCD9"/>
    <dbReference type="EnsemblPlants" id="KQJ90189">
        <property type="protein sequence ID" value="KQJ90189"/>
        <property type="gene ID" value="BRADI_4g29947v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821849"/>
    <dbReference type="Gramene" id="KQJ90189">
        <property type="protein sequence ID" value="KQJ90189"/>
        <property type="gene ID" value="BRADI_4g29947v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821849"/>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LCD9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09839">
        <property type="entry name" value="M1_like_TAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="8">
        <location>
            <begin position="382"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="9">
        <location>
            <begin position="598"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="10">
        <location>
            <begin position="744"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="122"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="690"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1094"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1148"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1240"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="169"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1104"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1161"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1271"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1294"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1304"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1329"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1345"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1360"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1371"/>
            <end position="1382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ90189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1407" mass="157147" checksum="02DCD895850025E3" modified="2016-01-20" version="1">MAKARKQKPEGGAGAGGGATVLHQKLCLSIDMENQLIYGYTEIRVVLAEKDTFALHADCMTIRNILVDGEAAEFEYSPHWKNVDDQPSWLSVSCLKTAADAACSAYVSSLTSEAVPNLIISSERSVKSTNEQPGEENGEKHEEKGGNPVQSSDDKAVKVCNGSAEENDKEVNKENKEEAEKENGKDEKNEMETEKEMKKDKEKEEEEEEEKKDEKEEKEDEKEDVIENEKDKENEMQIDDDKVKNTKVVRVDYILEKAETGVHFVNKVLHSNSQLRRAHCWFPCIDSATQRCPFDLEFTVSTDLVAVSNGDLLYQVLSKEDPPRKTYVYKLNTPVSAQWISLVVGPFEVLPDRNGISVSHMCLSPALLKLENTISFFHDAYSCYEDYLAAPFPFGLYKQIFLPSEMIVSPTSFGASMCIFSSDILNEEKVIDQIIDTRIKLAYALARQWFGIYTSAEEPNDEWLLDGLAGFLTEIFIKRYLGNNEARYRRFKANCTVCQFDISGATALGSPTASTDLYGTQTIGSYGKIRSLKAVAVLQTLEKQMGPDSFRKILQMIVASTRASRTLSTKEFRHLANKVGNLERPFLKEFFPRWVESCGCPVMRLGISYSKRRNVVELAVSRGCTAKADPGSDSHVNDDIQDSANGFPGMMSVRVHETDGVYDHPILPMAGEALQVVELQCHSKLAAKRFQKTKKGSKPDGSDENVEASTQENRTSMDSPLLWIRVDPEMEYLAEIHFHQPIQMWINQLEKDKDVISQSQAIAVLEKLPQISFAVINALNNFLNDTKAFWRVRVEAAYALAVTASEDTDLTGLLHLVKFYKSRRFDADIGLPRPNDFNDIPEYFVLEAIPHAVALVRSSDKSSPRQAIEFVLQLLKYNDNNGNVYSDVYWLAAMVQAIGELEFGQQGIGLLSSLLKRIDRLLQFDNFMPGYNGVLTVSCIRALARIAERVSSSICIDRICELIAPFRNIDKPWKVQIEASRVLIDLEHHHKGLDAALLLFLKYTYEEKSLQGGTKLAVHVLRLCQANVEPDLTDQIKLPTVVGLLHLLGSRKAYNNVFLRHNVFCILQIAAGRSPTLHGVSKLVAPSPLVQEISSDQHAKADSSVPQLSRPQEPSSSTPSVREVLLTTGPSKDADNISNCSERRNVVRIRVKRTTSSSKADGADHRDGLHGGRNENEAGPCSSMSVDAPMVGAPNEPLNLSDHNIEEQNSCHDRESRMSASVSNAKLLDKHEISKELQCTADSRLDSVPKDQFSPVTNVQEGVDKPGSQLEGVSTSYVGTQAPESINGLDAKEKKRKDKKDKKRNRDERRDKKDDPEYLEKKRLKKEKKKTEKELARKQKEGDRVSSQQRIVKPSDSQGTLAATLTPPATVQSAEPQVSNKDVTVDTARTPPTKIVKIKIKPLKKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3MBP9</accession>
    <name>A0A0Q3MBP9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100828898</name>
        <name evidence="3" type="ORF">BRADI_3g57763v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
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    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK01718"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">BRADI_1g42301v3</name>
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    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g42301v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18406">
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        <property type="gene ID" value="BRADI_1g42301v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="1738567at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
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            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK18406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="114" mass="12282" checksum="95E13F1DCC6B3275" modified="2016-01-20" version="1">MATATPAVPLPDYQVSGAAAHQRNGPGSSSSVGLGPFFGMLAAVLLLTALSCFFGRVCAAHAEGPDEWYDCTRLTAGRRRCWWRRRWRAPRRPAEEAKQPPAAPPPPALGWVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NTK8</accession>
    <name>A0A0Q3NTK8_BRADI</name>
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        <recommendedName>
            <fullName evidence="5">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g56834v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NTK8"/>
    <dbReference type="EnsemblPlants" id="KQK20798">
        <property type="protein sequence ID" value="KQK20798"/>
        <property type="gene ID" value="BRADI_1g56834v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20798">
        <property type="protein sequence ID" value="KQK20798"/>
        <property type="gene ID" value="BRADI_1g56834v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NTK8"/>
    <dbReference type="OrthoDB" id="681927at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835:SF60">
        <property type="entry name" value="OS10G0490300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK20798"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="816" mass="91034" checksum="A6D5A24676D52FCB" modified="2016-01-20" version="1">MGFQRKKAAGIKTWYHASFASFSSEEDTEKQGTPPKNKKCRKRKQDVSSSSSTAHDKMEHRRTGTKRKKPRNDATPKQVVGWKFSPSMLKKLLLRFDDEQKKWVNSTGFGVLYYVCDSQLPRELTLMLINKVNHHTGALEIDGVSVPMKPFVRKLLGIPNGKSPVTAPLFTVRGKTKVMKPTAEHKEFTDLKGGRGRPIHEVLDELVNCHEELKFQKKFIVVALSIYLAPRSGYLLNRTYLECLKNMALVADMNWCDYVADFLIQGIRDYHESGAANIFVHGCVHILVLMYIDLLEGPVPDHTLQFPRIQACTPEVLKQLDSIHPTAQARYRSLNEFTTLYGMDMADLDVPVRAQPVPCPEFTQQSADLPETTGPQQHASRQPGRHDSVPLDDTSQGLTRIDQQYKRDREHIIDECMTDMKRRIQKLNATRAAAIESLLRRVKASTRTNDPGGSGSVHDDVRTHSPHFDATPADAPTACPLRRSSDPAVIYPPNFDKTPIPNNMSSDNTVREQGQQPKMNLETDRGPEGSFFQDLGTPELMAQVLSIADDASKEAEQNAAHDMDAAVLQQKIAAIREPQNTSPVPSETHGQNLNGEQQHMVQPETVTNNTPKPHVPDQGMQPMFVADTFERQDPENSSSQLYEMSEQEIHTSSVHATDPAGQAAPTASMDVLTEPQLQNLIPPHLAGQLRSGQPELRMQTPFPVSDQCQLSLTDDYIERISSARSPVLPPNHESNDGPNASASLDLNASTVDDTFTGLHVDSQDQLEASLGTFEDDTQIRFDDAIYDHYAGYTPSKSHMIIAPLSNFIALLMYRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3P7D3</accession>
    <name>A0A0Q3P7D3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_5g23560v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3P7D3"/>
    <dbReference type="FunCoup" id="A0A0Q3P7D3">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3P7D3"/>
    <dbReference type="EnsemblPlants" id="KQJ84897">
        <property type="protein sequence ID" value="KQJ84897"/>
        <property type="gene ID" value="BRADI_5g23560v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ84897">
        <property type="protein sequence ID" value="KQJ84897"/>
        <property type="gene ID" value="BRADI_5g23560v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3P7D3"/>
    <dbReference type="OrthoDB" id="423313at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3P7D3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd03031">
        <property type="entry name" value="GRX_GRX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45669">
        <property type="entry name" value="GLUTAREDOXIN DOMAIN-CONTAINING CYSTEINE-RICH PROTEIN CG12206-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45669:SF30">
        <property type="entry name" value="OS04G0641300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23733">
        <property type="entry name" value="GRXCR1-2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="2">
        <location>
            <begin position="263"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ84897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="414" mass="44141" checksum="8981EC59DDB4084E" modified="2016-01-20" version="1">MGCATSVEAHREMRWVEPPRSSRSFSLPSVDRQRLRAKAASVLGHITAPGRRSGAGGGPTASCKYAATMATLSMEEIMMKLENDRTLAAVLAAVNAKETSAVVTRPGTPPNEPEPEVINAWELMAGLEEEDAPTPRVAAARDQIQTPPLWMMQAGNNADVPPIAFEFDQEILSGFREALAADTSPSAKEEPAQQEKKKDADADADADAGAGTTVSRARASDDMPAELAGIVRARINAFQEKIGRRNNGGTGRVPPGAKRKAAVVYFTSLRGVRKTFVDCCAVRSILRGYGVRVDERDVSMHAAFKAELARLLPGATAPLPRVFVDGRCLGGAEDVHALHEAGELARALEGCEAAPARKLGCMEACAACGDVRFVPCETCKVFVVEDGDEEEDGEFRRCPDCNENGLIGCPVCCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RPB8</accession>
    <name>A0A0Q3RPB8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g18880v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RPB8"/>
    <dbReference type="FunCoup" id="A0A0Q3RPB8">
        <property type="interactions" value="700"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3RPB8"/>
    <dbReference type="EnsemblPlants" id="KQK14834">
        <property type="protein sequence ID" value="KQK14834"/>
        <property type="gene ID" value="BRADI_1g18880v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14834">
        <property type="protein sequence ID" value="KQK14834"/>
        <property type="gene ID" value="BRADI_1g18880v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3RPB8"/>
    <dbReference type="OrthoDB" id="666348at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RPB8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33167:SF52">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33167">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE (DUF863)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="113" mass="12964" checksum="3786DFE4061E348D" modified="2016-01-20" version="1" fragment="single">MEKEQMKMAILRQEQTFRHQVNELHRVYQVQKQLMTEVHVVKMNWAQAGGDTQAEAMVETNHLQCYNFDLELTLATGSDRRKQEMASNSDSGATVSSSTSAESELRQRFPESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S2B6</accession>
    <name>A0A0Q3S2B6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">hydroxycinnamoyl-CoA:5-hydroxyanthranilate N-hydroxycinnamoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.302</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100822605</name>
        <name evidence="8" type="ORF">BRADI_1g47232v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of avenanthramide phytoalexins, which are phenolic alkaloids found mainly in oats. Catalyzes the N-acylation of 5-hydroxyanthranilate with 4-coumaroyl-CoA or caffeoyl-CoA as acyl donors, forming avenanthramide A and avenanthramide C, respectively. Does not accept feruloyl-CoA as a substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>5-hydroxyanthranilate + (E)-4-coumaroyl-CoA = avenanthramide A + CoA</text>
            <dbReference type="Rhea" id="RHEA:66932"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:167463"/>
            <dbReference type="ChEBI" id="CHEBI:167464"/>
            <dbReference type="EC" id="2.3.1.302"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:66933"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-hydroxyanthranilate + (E)-caffeoyl-CoA = avenanthramide C + CoA</text>
            <dbReference type="Rhea" id="RHEA:66936"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:87136"/>
            <dbReference type="ChEBI" id="CHEBI:167463"/>
            <dbReference type="ChEBI" id="CHEBI:167577"/>
            <dbReference type="EC" id="2.3.1.302"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:66937"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.302" evidence="7"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560989.2">
        <property type="nucleotide sequence ID" value="XM_003560941.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3S2B6"/>
    <dbReference type="FunCoup" id="A0A0Q3S2B6">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3S2B6"/>
    <dbReference type="EnsemblPlants" id="KQK19258">
        <property type="protein sequence ID" value="KQK19258"/>
        <property type="gene ID" value="BRADI_1g47232v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822605"/>
    <dbReference type="Gramene" id="KQK19258">
        <property type="protein sequence ID" value="KQK19258"/>
        <property type="gene ID" value="BRADI_1g47232v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822605"/>
    <dbReference type="OrthoDB" id="671439at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3S2B6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050734">
        <property type="term" value="F:hydroxycinnamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000015">
        <property type="entry name" value="Spermidine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF138">
        <property type="entry name" value="PUTRESCINE HYDROXYCINNAMOYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00051355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK19258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="474" mass="51128" checksum="34D7A105A097779D" modified="2016-01-20" version="1">MFKWECYSAVQQPSRQVPPSTSPALLAHSLPDREMAVEILESCMVTPSEATPTNAVWLSNLDLLVARGHTPTVYMYRPSSDRPDTAFFSPDVLKASLSKALVPFYPLAGRLDQDAAGRPEIHCGGEGALFVTARANATLDDLGDFAPSDELRRTLVPSADAEAGVHAGILAMFQVTFFKCGGVCLGAAIHHTAADGLAALDFVNSWATIARGGDDDVRGVLPCLDRTLLRARSPPSVRFDHAEYSRRGDVSGGPSKPTRVPFDSAILPMSKAQIDVLKSGEHGKRLSTFKAVVAHVWRSACEARRLDGAEDTRLYITADARSRVQPPLPRGFFGNAIFRASAAAKVEDVVGPGNGPPRLEPVAEMVAGATARLDDEYVRSLVDYLGLEDAAGLRKGEWVMPETDLWVISWQGLPIYGADFGWGSPAFMGRACLQFSGLVYLVPGPDGDGRLDVVVAMEPGSLVRFREVFYRGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CI88</accession>
    <name>A0A2K2CI88_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">histone acetyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_5g20065v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.48" evidence="1"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CI88"/>
    <dbReference type="STRING" id="15368.A0A2K2CI88"/>
    <dbReference type="EnsemblPlants" id="PNT61739">
        <property type="protein sequence ID" value="PNT61739"/>
        <property type="gene ID" value="BRADI_5g20065v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61739">
        <property type="protein sequence ID" value="PNT61739"/>
        <property type="gene ID" value="BRADI_5g20065v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CI88"/>
    <dbReference type="OrthoDB" id="47374at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CI88">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045141">
        <property type="entry name" value="NAA60-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14744">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14744:SF15">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT61739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="162" mass="18560" checksum="0B74A93F8361EB70" modified="2018-03-28" version="1">MKIVPAEDSEIEDLFSYNNSQKGLTLAYILMLGVVDHYRNLGIGVYICMSFHTINLPPASMRRCYLIWFKDCRCSTTLKDSITTLYLFAYYVNGHQSPCSLLEIAASFAVHFRGLLEVLVTPLWSNKDQNTHELSRFKESSTLVITRNDARIVSSEDMRRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CKP0</accession>
    <name>A0A2K2CKP0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA-processing protein FYV7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100834127</name>
        <name evidence="3" type="ORF">BRADI_4g05720v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CKP0"/>
    <dbReference type="STRING" id="15368.A0A2K2CKP0"/>
    <dbReference type="EnsemblPlants" id="PNT62596">
        <property type="protein sequence ID" value="PNT62596"/>
        <property type="gene ID" value="BRADI_4g05720v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62596">
        <property type="protein sequence ID" value="PNT62596"/>
        <property type="gene ID" value="BRADI_4g05720v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1928808at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CKP0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013730">
        <property type="entry name" value="Fyv7/TAP26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15657">
        <property type="entry name" value="THYROID TRANSCRIPTION FACTOR 1-ASSOCIATED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15657:SF1">
        <property type="entry name" value="THYROID TRANSCRIPTION FACTOR 1-ASSOCIATED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08524">
        <property type="entry name" value="rRNA_processing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="125"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT62596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="32548" checksum="FEF3AA4D07A4A1D5" modified="2018-03-28" version="1">MKRPPPRDDAAAAGSSGGGGGFKKGNGRWGGKKRNEQRLGGSGGALSLAAFANAKSRNTGYNPALIKKQKEFYRNAKLISKYKRSRKQQNQSNYTPQVPTCEEGDVDAEDEPKTHDKRKKRTSQSLKVEYEKKRLEDEKAKKERDAIIQAKKEEREKSEAKRKELREKMFKRTRSGQPVMKYRIEHLLETALESSNKPWCFVRCRRCSSSCLWRASLEPWRPSSGWILPISPWGSWISSLRWQVQEAPWRTLWWQVQAWQVLRRCRLPPWETQEVEIEAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CLJ5</accession>
    <name>A0A2K2CLJ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC112268611</name>
        <name evidence="3" type="ORF">BRADI_4g09301v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310224.1">
        <property type="nucleotide sequence ID" value="XM_024454456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CLJ5"/>
    <dbReference type="EnsemblPlants" id="PNT62897">
        <property type="protein sequence ID" value="PNT62897"/>
        <property type="gene ID" value="BRADI_4g09301v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112268611"/>
    <dbReference type="Gramene" id="PNT62897">
        <property type="protein sequence ID" value="PNT62897"/>
        <property type="gene ID" value="BRADI_4g09301v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT62897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25469" checksum="7A762FBC74E495F2" modified="2018-03-28" version="1">MLLVCRSCIGRMDHAARIIWWNHHYMFGVLVLRCIADFCGSPEEWPTPATYHVSLYQLRVLDHDGRTRLRRSSQRQGIGAGRPKSAKFAVALAATTSVFTGAIFMAVFFIWRSSLPKVFSENEEVIYRWSRKVGVSARCHCLLRQYRASSALRARGRKKKSAAVPAENAEASAPAENAEDWPAPDVEDWLAEEWTDPNAEVAAENVEAEVAAENVQAPNAAEVEAPNVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQ26</accession>
    <name>A0A2K2CQ26_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g24722v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQ26"/>
    <dbReference type="STRING" id="15368.A0A2K2CQ26"/>
    <dbReference type="EnsemblPlants" id="PNT64124">
        <property type="protein sequence ID" value="PNT64124"/>
        <property type="gene ID" value="BRADI_4g24722v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64124">
        <property type="protein sequence ID" value="PNT64124"/>
        <property type="gene ID" value="BRADI_4g24722v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQ26"/>
    <dbReference type="OrthoDB" id="781981at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000427">
        <property type="term" value="C:plastid-encoded plastid RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044710">
        <property type="entry name" value="PTAC6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35994">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35994:SF1">
        <property type="entry name" value="PLASTID TRANSCRIPTIONALLY ACTIVE PROTEIN 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT64124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="71" mass="8331" checksum="045D168C866FA7C7" modified="2018-03-28" version="1">MYVTPPYTDVYSIPRVLEPMPQKYVRCVKKDYGCYHASEPPVEHSCDPLYVIESKIMMVCVRMFPVLLIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQB2</accession>
    <name>A0A2K2CQB2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4220 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g26119v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQB2"/>
    <dbReference type="STRING" id="15368.A0A2K2CQB2"/>
    <dbReference type="EnsemblPlants" id="PNT64210">
        <property type="protein sequence ID" value="PNT64210"/>
        <property type="gene ID" value="BRADI_4g26119v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64210">
        <property type="protein sequence ID" value="PNT64210"/>
        <property type="gene ID" value="BRADI_4g26119v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQB2"/>
    <dbReference type="OrthoDB" id="1689146at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025315">
        <property type="entry name" value="DUF4220"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007658">
        <property type="entry name" value="DUF594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31325">
        <property type="entry name" value="OS01G0798800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13968">
        <property type="entry name" value="DUF4220"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04578">
        <property type="entry name" value="DUF594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4220" evidence="2">
        <location>
            <begin position="41"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4220" evidence="2">
        <location>
            <begin position="170"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT64210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="494" mass="55829" checksum="6AC4CF70FCDEB33C" modified="2018-03-28" version="1">MESLVQIFNEWQIQLLVLLSFTLQVFLFFTGGLRWRSTDWYMVAIYALGFLSRHHKEVTPGKETLRQAHPLAFLWAPFLLMHLGGQDTVTAFAIEDNNLWLRHLLNLVVQVAVALYVFWVSMTGRGDRSVQLPGIFVFIAGVIKYGERTLAQYGRREVLTSDIHVDHLPKLLEIELDLMYDDIYTKAMVLRTRSGIIFRCVSQASMVVGFVLFIVGAVNNKHGYSRADLAITYVLFTGGLCMEVCAVFILLMSPWTWAWLKARNNHGLLACISSFLLSSNIGRPERRPLWSNSMGQYNFLSYVSSDQQSRLYKFAKKVVAASLLVVGAGKKVELSLFLSMSKLLESDHERRKLPYLGPLLAKLIPDFGASFGYGIVCFHIFTEAHLRKYTYPGRDHVLVSACRKLSNYMLYLLATHPEMLPVSGSIEALVEMRDLWTTLLIFSAGKSRADVHAAQLSRGGELLTFAWLLMAHRKLGDVGEAYNFNLLPIKPGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CRB0</accession>
    <name>A0A2K2CRB0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_4g30081v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CRB0"/>
    <dbReference type="STRING" id="15368.A0A2K2CRB0"/>
    <dbReference type="EnsemblPlants" id="PNT64543">
        <property type="protein sequence ID" value="PNT64543"/>
        <property type="gene ID" value="BRADI_4g30081v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64543">
        <property type="protein sequence ID" value="PNT64543"/>
        <property type="gene ID" value="BRADI_4g30081v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CRB0"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CRB0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT64543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="145" mass="15883" checksum="B074819A66E08263" modified="2018-03-28" version="1" fragment="single">VRFSKRRAGLFKKAFELAILCDAEVALLVFSPAGKLYEYASSSIEGTYDRYQQFAGAVRNKYRGGASTSNNEDPSDIQSRLREITAWSLHNNTDEADASNLEKLEKLLTDALRITESKKTLAKQNSNRSATGENSNGPTGEGGNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CU57</accession>
    <name>A0A2K2CU57_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100826291</name>
        <name evidence="4" type="ORF">BRADI_4g44300v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024319041.1">
        <property type="nucleotide sequence ID" value="XM_024463273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CU57"/>
    <dbReference type="FunCoup" id="A0A2K2CU57">
        <property type="interactions" value="366"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CU57"/>
    <dbReference type="EnsemblPlants" id="PNT65555">
        <property type="protein sequence ID" value="PNT65555"/>
        <property type="gene ID" value="BRADI_4g44300v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826291"/>
    <dbReference type="Gramene" id="PNT65555">
        <property type="protein sequence ID" value="PNT65555"/>
        <property type="gene ID" value="BRADI_4g44300v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19657at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CU57">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43689:SF1">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43689">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
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        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="134"/>
            <end position="457"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
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            <end position="284"/>
        </location>
    </feature>
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        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
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            <end position="275"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT65555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="480" mass="50772" checksum="6355B69BB91F0674" modified="2018-03-28" version="1">MRAALLLRPPPPPPAAGNLLSAASGRPPPPLLLRARRMAAGPPPPSAAAADFPAANVSGPVPDDGGARTRPRRRRSVAGVDQDELLDPGALADPDSSFCDISGVSFHHKVYSAAHHEEQQDPASASGSGVGVPVVLLHGFGASLFSWSRVMRPLARAAGAKVLAFDRPAFGLTSRAAWSGSGPGGDNDDDTRPLNPYSMAFSAMATLAFVDLLGAGKAVLIGHSAGCLVAVDACLEAPERVAALVLVAPAIFAPGSGTKGERREDGEEGQEKKDSDDDSNGPPLNPFARIWRGFLGMCMWIAGLVLKVVMAVQDVVRALAVQALVALLRSPLGAIMVRLVMDKFGVMAVRNAWYDPSKVTDHVIQGYTKPLRSRGWERALLEHAISMITDSAPKSRLPVSKRLSEISCPVLVVTGDTDRLVPAWNAERVARAIPGAVFEVIKNCGHLPHEEKPEEFLSIVEGFLRRAFGTPDEQVLQAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CUI1</accession>
    <name>A0A2K2CUI1_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g00945v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT65691">
        <property type="protein sequence ID" value="PNT65691"/>
        <property type="gene ID" value="BRADI_3g00945v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g00945v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CUI1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036043256" evidence="1">
        <location>
            <begin position="17"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT65691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="168" mass="18909" checksum="B032513C0185CD19" modified="2018-03-28" version="1">MDLVLVLHLWTQGGFSLLMSPDDFCTWACFLREANQPAIHASPRVPPVQVTSYSGQISIFNRRDFRDLFQIESVIVIRNGDCLTRRSKPIKAKTLNLSRSLSGSLLKEVIGLWFFSLTCHKLVFAKLHAGSLKEATDWISNSIAKVLLVITASSNHLATERRSYFSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CX86</accession>
    <name>A0A2K2CX86_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g15112v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CX86"/>
    <dbReference type="EnsemblPlants" id="PNT66643">
        <property type="protein sequence ID" value="PNT66643"/>
        <property type="gene ID" value="BRADI_3g15112v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66643">
        <property type="protein sequence ID" value="PNT66643"/>
        <property type="gene ID" value="BRADI_3g15112v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CX86"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="179" mass="19570" checksum="0034F843A4A847DB" modified="2018-03-28" version="1">MVDKFAKWIESDRARPHASRPSRPGRPARAPAPADRWRPPVRGFFPGRKVPRPPRAILLRRRALTTGFPSLRPSFCTPERVLLSPANPSLLPLYSPSLSRCQAARFLAGVRPPAPPSTDCAARPSRPRRRPRPPEPTPTAPPSPPCCAAPSPATPATSASPEDPAPPRPCPRRPPLRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D205</accession>
    <name>A0A2K2D205_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein FAM91A1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100842470</name>
        <name evidence="5" type="ORF">BRADI_3g38460v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM91 family.</text>
    </comment>
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        <property type="protein sequence ID" value="PNT68301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2D205"/>
    <dbReference type="EnsemblPlants" id="PNT68301">
        <property type="protein sequence ID" value="PNT68301"/>
        <property type="gene ID" value="BRADI_3g38460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842470"/>
    <dbReference type="Gramene" id="PNT68301">
        <property type="protein sequence ID" value="PNT68301"/>
        <property type="gene ID" value="BRADI_3g38460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842470"/>
    <dbReference type="OrthoDB" id="275996at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="entry name" value="FAM91"/>
    </dbReference>
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        <property type="entry name" value="FAM91_C_dom"/>
    </dbReference>
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        <property type="entry name" value="FAM91_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441:SF1">
        <property type="entry name" value="OS05G0355133 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441">
        <property type="entry name" value="PROTEIN FAM91A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14648">
        <property type="entry name" value="FAM91_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14647">
        <property type="entry name" value="FAM91_N"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="98"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="381"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="578"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="803"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="482"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="492"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="544"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT68301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1020" mass="111694" checksum="D321F5BD8BBD8EFC" modified="2018-03-28" version="1">MRSNPNYKHTPMCPRPPLPHAPFVEVRIGAAAACIVVRSSQTIEATGERGQDVEDAATDGGDGGGADDGEGDQGRVPVGDPLQAHPGRRRLQGGVAPKLYPYHLADYICRVMRISSFRYYCDVLFEAMKNEQPYDSIPNFTAADALRITGVGRNEFIDIMNKCRSKKIMWKLNKSIAKELLPAQPADIAIEPWWGVRFVNFTLEEFKKLSEEETSAIDKICKEEVNSYVLFDPEVINGLYKRGGVYYDVPVYPDDRFRVSRLEGFVSNKDQSYEDPIEELLYAVFVVSSENATVAELAKTLQADLYQLQSAASFACRLGWAVKLMDTDSVLRDSSTAALPINILGDDDDGSRTSINSEKSCQELLSNDSDGPRKISGTAYVGFVVDANVTSYLMMGSLSPGLKSHAVTLYEAGKLGYSCIADLCNDLASLEGKKFEGVLQEFANHAFSLRCFLECLLSGGTSPTESSEGNYQVCHLPDDLSSSLSKEGTEEGVGNVIENGGVSHKTRDPADDGQDGDISQQEHTTIDSDGSPHSTTISERKESIVNNDFDGSQTVTQKDSSQTTELDGLAGNTSVPKTKRNYRVNILRCESLASLAPSTLERLLLRDYDIVVSMIPLPYSSVIPSSAGPVHFGPPSYSSMTPWMKLALYTSGSCGPISAIFMKGQRLRLLPEPLASCEKALIWSWDHSVVGGLGGIFEGNLVKGSLLLHYLNSMTKHSAVIVQPLSINDLDETGNLVTMDVPLPLKDVDGSIASVIAGTDLPEEQMSNLTSLLEDLSFKVELSTVGYLRLVRLHRLSESFELTEKESYEWIPLSLEFGIPLFNPNLCERICERVFASHILQKDDISEHYESMQNVRKRLRELCTEYQATGPTARLLNQRGGGSKTSPRQLMNIVSGRWSPFHDSLTPTNGGSPHEHVRLNLGRRQKCFTEVLSFDGSILRSHALAPVYEAATRSVTEDQPSPSAVKLESEDSGTKDVVLPGVNLIFDGAELHPFDISACLQARQPISLIAEASAASLAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D5F6</accession>
    <name>A0A2K2D5F6_BRADI</name>
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        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_3g56980v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D5F6"/>
    <dbReference type="STRING" id="15368.A0A2K2D5F6"/>
    <dbReference type="EnsemblPlants" id="PNT69520">
        <property type="protein sequence ID" value="PNT69520"/>
        <property type="gene ID" value="BRADI_3g56980v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69520">
        <property type="protein sequence ID" value="PNT69520"/>
        <property type="gene ID" value="BRADI_3g56980v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D5F6"/>
    <dbReference type="OrthoDB" id="589312at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D5F6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
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        <property type="entry name" value="F-box_dom"/>
    </dbReference>
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        <property type="entry name" value="F-box_plant"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32153">
        <property type="entry name" value="OJ000223_09.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNI-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="26"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="3">
        <location>
            <begin position="163"/>
            <end position="275"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT69520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="386" mass="44372" checksum="9838B8812F61BAC3" modified="2018-03-28" version="1">MKNRKGRRSRNKATCNEAATSNGDRFSTLPDDILLNVLERVETLDALRTCILSKRMLKLPTMLSHIDVDIYSLAYYGSFRDLVRINGAVADVTENILSTRSPEIPIRKLKVRFVLRHYDFLSIVKSFARAMATQKVEKAEIEIMTEKIKYQYCTPADRLDSAKKFNTILDACPDAFPGLTSLWLHNMSFGELDIPNILSTCKRLQSFHLTYCDTGTRSVLQIEHDQLIELEIDYGKFETVKLTYLPKLQQVSYNNWCYEDQPLSFGFVPQLSKLSLTRTAVRSDRALELSQVLANFPSISELHLDFQSEKSLFAASPKFLICFFHPRFGFYQNARNCLRLCLANYGLWIWTIFLKDVILLGQCSFFKLHPSLRSYASQYGIIGATW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D6H0</accession>
    <name>A0A2K2D6H0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC104582484</name>
        <name evidence="1" type="ORF">BRADI_2g02084v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT69879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT69880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230410.1">
        <property type="nucleotide sequence ID" value="XM_010232108.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314126.1">
        <property type="nucleotide sequence ID" value="XM_024458358.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314127.1">
        <property type="nucleotide sequence ID" value="XM_024458359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D6H0"/>
    <dbReference type="EnsemblPlants" id="PNT69879">
        <property type="protein sequence ID" value="PNT69879"/>
        <property type="gene ID" value="BRADI_2g02084v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69880">
        <property type="protein sequence ID" value="PNT69880"/>
        <property type="gene ID" value="BRADI_2g02084v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104582484"/>
    <dbReference type="Gramene" id="PNT69879">
        <property type="protein sequence ID" value="PNT69879"/>
        <property type="gene ID" value="BRADI_2g02084v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69880">
        <property type="protein sequence ID" value="PNT69880"/>
        <property type="gene ID" value="BRADI_2g02084v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104582484"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D6H0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT69879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="214" mass="24619" checksum="551A70C9D0EC40DF" modified="2018-03-28" version="1">MTKLPITPCNYYRCCCIENLLPDGRSRQTAHSIHTTIAALDQPRRWSHTSRMSYLCLWHHNCTHAACVPREMESLEMTPMGEYIWTVSTRMSTARQSCSQIIMLLVPGKEKMRFFLFIPFFLWAANCRAGDESFSLRSLVRLSDESACAPCLRWHRMAVRIGFQMAKWEELCPCAVPARSKFTLEPLNIVEGTRRGAVRRRNRVSSVLDRAGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D6U2</accession>
    <name>A0A2K2D6U2_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100835007</name>
        <name evidence="2" type="ORF">BRADI_2g04151v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT69999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232986.1">
        <property type="nucleotide sequence ID" value="XM_010234684.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D6U2"/>
    <dbReference type="EnsemblPlants" id="PNT69999">
        <property type="protein sequence ID" value="PNT69999"/>
        <property type="gene ID" value="BRADI_2g04151v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835007"/>
    <dbReference type="Gramene" id="PNT69999">
        <property type="protein sequence ID" value="PNT69999"/>
        <property type="gene ID" value="BRADI_2g04151v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835007"/>
    <dbReference type="OrthoDB" id="994901at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D6U2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37722">
        <property type="entry name" value="OS01G0167700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37722:SF2">
        <property type="entry name" value="OS01G0167700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="769"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="769"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="815"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT69999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="958" mass="108109" checksum="C9BEC2AD2E37C9DE" modified="2018-03-28" version="1">MLQWMGGSRRKVYTSRKSIQSRQRQYFEQKKRRQQQREGLQNQDDIDGAGNQAYGDQAPRSLDVLSLNNLATPVCHRNGPENVDSTVLLPQMDCTILNPSPIEALKKITSVYNINPKETSSQPRLSSPIGHQDVAVSVNPHEDPVVRKISPSKNSGVKKRNLNVDLHSGEISLIDLVNYEGPNNKSTAQPAREPHVSFFVKGLGHVEMETPPQSPRSTKRVLPLPPKAMRYTQNKTRRSIPFDATKGLDSILKGISMMKERISSDKMGSLVDESNYERRKNSYSSHSFENYNGNLYPEDEDMFCEPQAQKGWQSKHGRIVDSLPAINSERLWKMESFNSDDHFGTPRAEQFDPVDYGFKERYSPEQRTLIGTSSRFQTSANPSGHDHFSDQLLLDDDNDMVHFDWERHPPIKKIPNSNSTFGPAAWSFDMVDDSDKRRSPLSEESCSSAAAMNDRTNKKPLLSVKCEENNMNKKDDFHTRLDKLDIPEMDAHLDGISLFGDQEYHKRTTDLRNLEASYCPDKAIKQQRTREPNCRLSLQEKFADWGSSTSHLKGSTGLHNPSNCTGMHEDKPFNSIPDVGGYHTVGSTERRPAPRVHPVSHRPDNANLYDDTRLQNPVSDTFGDNIEFSDPFCGKDLQSNIDACTFLGQKVDRKKEDDFDPFKNPNADIFHSAGSVGQSVNRKKEDNFDPFKNPNADVFHSAGSVGQSVVGQHTTCSQQSARDELRQGFNPGIDFQETRLNSFWEDGHVSNGTFRGDIELSDLLARENGEKNKDKIERSEKPETAQPSADFRIKMSEAETCSDGSEVTNCPGVQKETSTAATQPPANLSCLQDTSRGMFEIHARVDCIRKEMIESPVIDFEAPLHVRNKIHDVGDHTKRNYMFQSPFAAEKVGIEKKVITGVSPNNSDVQYEVMLERRVLQRLSVQKILVDTPIKDKLDKVTHFRRMEDGSHVLARSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D832</accession>
    <name>A0A2K2D832_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g11814v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D832"/>
    <dbReference type="STRING" id="15368.A0A2K2D832"/>
    <dbReference type="EnsemblPlants" id="PNT70437">
        <property type="protein sequence ID" value="PNT70437"/>
        <property type="gene ID" value="BRADI_2g11814v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70437">
        <property type="protein sequence ID" value="PNT70437"/>
        <property type="gene ID" value="BRADI_2g11814v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D832"/>
    <dbReference type="OrthoDB" id="1909330at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT70437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="112" mass="12583" checksum="D067A99A1FF421F5" modified="2018-03-28" version="1">MLQSHVIQGFWLGLRCDFCNKHFPKHDIAIVLEDEDGHNYDTKNLEEESSNDVKSKEDPKVTRVRSKIVHSDINNLVCESIDGITFSESDIDFDDVASFSNVNIAAGSLVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D8X4</accession>
    <name>A0A2K2D8X4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g16695v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D8X4"/>
    <dbReference type="EnsemblPlants" id="PNT70719">
        <property type="protein sequence ID" value="PNT70719"/>
        <property type="gene ID" value="BRADI_2g16695v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70719">
        <property type="protein sequence ID" value="PNT70719"/>
        <property type="gene ID" value="BRADI_2g16695v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D8X4"/>
    <dbReference type="OrthoDB" id="688980at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT70719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="123" mass="13783" checksum="C1D4D8F6DD022283" modified="2018-03-28" version="1">MMAYISTAGAEAYGSCQEAYSKYTNYVSMAQEAHEPETIHDLDLEVVMREGGDMKHGRFFNGDGSIDPATAPSLAHIRARSTSSSPSIRTNMHDRCRSRSLQLQSKPWRSVSWGVCRLRCRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D9W5</accession>
    <name>A0A2K2D9W5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g22295v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D9W5"/>
    <dbReference type="STRING" id="15368.A0A2K2D9W5"/>
    <dbReference type="EnsemblPlants" id="PNT71057">
        <property type="protein sequence ID" value="PNT71057"/>
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        <property type="gene ID" value="BRADI_2g22295v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="674687at2759"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF48">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <end position="356"/>
        </location>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="583" mass="64356" checksum="E402244B6147F46A" modified="2018-03-28" version="1">MDGAPRNSAPAAVHADAAVARKIPNPESLKRKERCHRAAEAKKSQEDATARALAAAKEEDAIVAEAAHNQQLAAQNQQLAAQQALFLYNRQAVAAAFAQQAAVSQSSGNRLPRSMSPPVSSPTYGSGPAMGPFSPCIQSPEVGDSMSFVGAPSALDLNAMNSPGDSPMMQWMPPSARDLPDGRNLFSSPSPTAVSLDARNVFDELSPTNEAYYDDEESLQEMIRHGGQQDGRQEEDEDGGHSGQQDDELDGEENGEQDEEFDYMGKSTFEEELAQVASETASRTKRTGSYTEDEDKLLCDAWLTIGQDPIARAEQKGGAYWKRIHEYFHKYKSHIPYSFESDRNENSLTHRWQHILAECNKYTAAYNSVKNRPVSGVGMASQVIQAMDAFRAMNNGKKFNLSHCWIKLHKAPKWIDLVASLKIQAKNAAQGKKRSNDGIPIDLEAGEISGGLGKRSDRPQGKKLSKDDLKREASTIALQESLKEMVSVKEASSGKRGEIRELKKDERFKSFMDMIQEKYRGDATVATDLAAAAKLDATPRAKEVELKMLMEENRIIRMDLSPLDDVARAWYIKKKKEIADQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DIB9</accession>
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        <name type="common">Purple false brome</name>
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            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT74019.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT74019"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="102" mass="11486" checksum="33F57ECC38C06EEB" modified="2018-03-28" version="1">MAEKVSTLIMEANLELESVTARSQRTRCQIKKKPDEKEPDRRLRCPSRATATGAAPSFTRTRSRSPRRVMSTDDDGADAAGDMLRYRRRVREEEAGSPMIAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
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        <name type="synonym">Trachynia distachya</name>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="1 2" key="1">
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="BRADI_1g14615v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g14615v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DJI1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT74438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="73" mass="8135" checksum="1FD3B0334AED7B73" modified="2018-03-28" version="1">MLDLQELDSEISATIWRFFLSKGSGTLGKFLGRADGPVPSPVPHCTIYKIINILHLHHSTIIYVGFHSCKARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DL20</accession>
    <name>A0A2K2DL20_BRADI</name>
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        <recommendedName>
            <fullName evidence="5">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    <organism evidence="6">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A2K2DL20">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DL20"/>
    <dbReference type="EnsemblPlants" id="PNT74978">
        <property type="protein sequence ID" value="PNT74978"/>
        <property type="gene ID" value="BRADI_1g25622v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74978">
        <property type="protein sequence ID" value="PNT74978"/>
        <property type="gene ID" value="BRADI_1g25622v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DL20"/>
    <dbReference type="OrthoDB" id="673386at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DL20">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000004">
        <property type="entry name" value="Receptor-like serine-threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000006">
        <property type="entry name" value="Receptor-like serine-threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32099">
        <property type="entry name" value="CYSTEINE-RICH REPEAT SECRETORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32099:SF106">
        <property type="entry name" value="GNK2-HOMOLOGOUS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036319255" description="Gnk2-homologous domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="5">
        <location>
            <begin position="21"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="5">
        <location>
            <begin position="134"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT74978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="278" mass="28990" checksum="9C84B2D43F65A745" modified="2018-03-28" version="1">MAGHFILVALVLLLLAARAAAYPWQVCGKAGNNFTANSTYQANLGLLAAALPTNTSSSPGLFATAVVGADDDPVSALALCRGDVTNATLCSGCLATAFQDVQNMCAYDRDAAIYYDPCVLYYSSDAGFLSSADSLAMTNRINFRNVTTDPGGFNRQVAALVGATADYAAYNSTRRRYASGVADLDGGEFPRVYSWAQCTPDLTPAQCRGCLAGIIAQMPRLFTDRVGGRVLGVRCSYRYEVYSFLDGPVTVRLAAPAGGSKAPAPAPESTVDGGGVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DN08</accession>
    <name>A0A2K2DN08_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g36511v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DN08"/>
    <dbReference type="EnsemblPlants" id="PNT75668">
        <property type="protein sequence ID" value="PNT75668"/>
        <property type="gene ID" value="BRADI_1g36511v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT75669">
        <property type="protein sequence ID" value="PNT75669"/>
        <property type="gene ID" value="BRADI_1g36511v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75668">
        <property type="protein sequence ID" value="PNT75668"/>
        <property type="gene ID" value="BRADI_1g36511v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75669">
        <property type="protein sequence ID" value="PNT75669"/>
        <property type="gene ID" value="BRADI_1g36511v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DN08"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="76" mass="8195" checksum="B4E4E77D5B7EC9A9" modified="2018-03-28" version="1">MLCPAITLTAAPETKGLGTHLILVSLKLTLILLLFRILVLLAPDGWSAIIWVSSSFEPPEFVIFAKALKKPKPGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQA2</accession>
    <name>A0A2K2DQA2_BRADI</name>
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">BRADI_1g48305v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQA2"/>
    <dbReference type="FunCoup" id="A0A2K2DQA2">
        <property type="interactions" value="370"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DQA2"/>
    <dbReference type="EnsemblPlants" id="PNT76454">
        <property type="protein sequence ID" value="PNT76454"/>
        <property type="gene ID" value="BRADI_1g48305v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76454">
        <property type="protein sequence ID" value="PNT76454"/>
        <property type="gene ID" value="BRADI_1g48305v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DQA2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23340">
        <property type="entry name" value="beta-trefoil_FSCN_ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007679">
        <property type="entry name" value="DUF569"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054726">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31205">
        <property type="entry name" value="ACTIN CROSS-LINKING PROTEIN (DUF569)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31205:SF72">
        <property type="entry name" value="DUF569 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04601">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22932">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="39"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="DUF569" evidence="2">
        <location>
            <begin position="223"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT76454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="318" mass="35729" checksum="9BB031DD9A45AC9B" modified="2018-03-28" version="1">MDPVIRFLGLQVHSVLSKARTATSKYTALAPEQATGDAMNYFPDRQHVRLRSLELGTYLHAAADGIEVRLHPERASLNAADGIENEQSNTYLLLHSAASGRYLAADTNTRAPWGQRGFRVEQREFDEPEVVDSIMWQVIRPGNFVLLRHVSGRLLRANGARLFNWNTGVTVDKFENRSAMNRWVVEPIPPSQLYPGVPGPIAEPLSNPLFMCLLFGRESPPVRLIRFQQANADGTVNEDDWTEFQFRGNSSYRLRFQLVVRMYIVNFIMCVRAGRYARLTLILANLPRGATGATLYIVAVHSLSPGADELRYPDMDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQC4</accession>
    <name>A0A2K2DQC4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g48713v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQC4"/>
    <dbReference type="EnsemblPlants" id="PNT76487">
        <property type="protein sequence ID" value="PNT76487"/>
        <property type="gene ID" value="BRADI_1g48713v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76487">
        <property type="protein sequence ID" value="PNT76487"/>
        <property type="gene ID" value="BRADI_1g48713v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DQC4"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF369">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036319262" description="ABC transporter domain-containing protein" evidence="3">
        <location>
            <begin position="29"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT76487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11763" checksum="D16C06B2F3EEE638" modified="2018-03-28" version="1">MGQRQLFCLGRALLRRCCILVLDEATASVDNATDAVLQKTIRTEFKHCTVITVAHRIPTVMDCDMVLAMSDGRVVEYDKPIKLMETEGSLFCNLVKEYWSYASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DSZ7</accession>
    <name>A0A2K2DSZ7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g62033v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DSZ7"/>
    <dbReference type="EnsemblPlants" id="PNT77396">
        <property type="protein sequence ID" value="PNT77396"/>
        <property type="gene ID" value="BRADI_1g62033v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77397">
        <property type="protein sequence ID" value="PNT77397"/>
        <property type="gene ID" value="BRADI_1g62033v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77396">
        <property type="protein sequence ID" value="PNT77396"/>
        <property type="gene ID" value="BRADI_1g62033v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77397">
        <property type="protein sequence ID" value="PNT77397"/>
        <property type="gene ID" value="BRADI_1g62033v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DSZ7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT77396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="118" mass="13218" checksum="7A4F6D7E70BE5999" modified="2018-03-28" version="1" fragment="single">SRRLDCSAYLVSYDPLSRGIKVGCSLQTGRPSLCDKCWIWMPVFLVVGRVLSARERRRLDPVGFGAWMPMGLVMAMVFMAWEWLPSKEGATLPFWHFVRGCGGGHGWGWIGGLTRRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DT05</accession>
    <name>A0A2K2DT05_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g62200v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DT05"/>
    <dbReference type="FunCoup" id="A0A2K2DT05">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77411">
        <property type="protein sequence ID" value="PNT77411"/>
        <property type="gene ID" value="BRADI_1g62200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77411">
        <property type="protein sequence ID" value="PNT77411"/>
        <property type="gene ID" value="BRADI_1g62200v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DT05"/>
    <dbReference type="OrthoDB" id="683654at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT77411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="299" mass="32113" checksum="283F579DC9CEABBD" modified="2018-03-28" version="1">MDSSEHVLVSNPDKLVLLAEIPAADPRGSEPTATLRLLVELRSDHGAGLEDVDTMEDVTCRVPLRDLVRQGAADRAFGELVARLDNPMLRPDVTAATKAAAGLVRARCGDSAAVDGLSGMEFRLRVTFIDASDEPASDEDEDESGSDMEFGEFDLSGARTLHSQTFAGDDYEDDDDDEDGCGAQFSVRPYQGGFARAAGGFGGSRDRTSLLLSGFEARSDGPELTDQHELTSWDMQRVVRLALDDDGSLEDDEAYQRALAGGTPMSRASRAAMLGQALQSVRQPQSKSPSAMFPMRTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DUK3</accession>
    <name>A0A2K2DUK3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Btz domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100833545</name>
        <name evidence="2" type="ORF">BRADI_1g70960v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229012.1">
        <property type="nucleotide sequence ID" value="XM_010230710.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DUK3"/>
    <dbReference type="FunCoup" id="A0A2K2DUK3">
        <property type="interactions" value="1758"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DUK3"/>
    <dbReference type="EnsemblPlants" id="PNT77959">
        <property type="protein sequence ID" value="PNT77959"/>
        <property type="gene ID" value="BRADI_1g70960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833545"/>
    <dbReference type="Gramene" id="PNT77959">
        <property type="protein sequence ID" value="PNT77959"/>
        <property type="gene ID" value="BRADI_1g70960v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1920561at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DUK3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36364">
        <property type="entry name" value="OS03G0203000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36364:SF1">
        <property type="entry name" value="OS03G0203000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT77959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="40169" checksum="473CBD24537D56A8" modified="2018-03-28" version="1">MTTTREPREPSRREDRDSHGRRHHSSYRSRRDDPSPRRWRDDRRHESDRSQYRRRAEESANAGDRDERRNRPLQDAAQRNDPLPAEGKPLSDTKEDPPVRHEISPRGTKRFSETRESWRPRSSFFQHDERDSAGQRGRRVDRQGSEYGRQRDQKEHLGDRDKHKSEGHGLQGKAEQVHQQSDVESTWKHDGFFQLEEEAPAAKRRPAFREMGMPLEEQGSASVTEPDSRSRKPDQPGLSGMGEERRSHHSREFVRPDDRGTRWGFSDYRSAGQRNGYDSRGRFAGRGGRGRDRFNNSYDGRNMYQAAGDQEEKWKHDLYDQTNSTPAPLTEEEQIAKVEALLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DV19</accession>
    <name>A0A2K2DV19_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication protein A 70 kDa DNA-binding subunit B/D first OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g74109v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DV19"/>
    <dbReference type="STRING" id="15368.A0A2K2DV19"/>
    <dbReference type="EnsemblPlants" id="PNT78129">
        <property type="protein sequence ID" value="PNT78129"/>
        <property type="gene ID" value="BRADI_1g74109v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78129">
        <property type="protein sequence ID" value="PNT78129"/>
        <property type="gene ID" value="BRADI_1g74109v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DV19"/>
    <dbReference type="OrthoDB" id="686632at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48299">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48299:SF2">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="1">
        <location>
            <begin position="41"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT78129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="216" mass="24893" checksum="CF4B06DAC5590954" modified="2018-03-28" version="1">MAPLNLTRKRKYADFATPPPPPMFKDFDDIAMDDVFTNAKFSICVRVIVKFPANRYGDDMHFILMDKPGAKIEAIVAGNEQVNRFKDILESGKNYTIHNVTSCLNAEDIIFRNIGHTFECAFDRKTNVINCTMGIPFPLYPKEFTPYQEVHACPNKTFVDIVAIVIYYGDLEIVGRYPYAEQYREVIFMDLRLSRYIRAHHHCMEPRSHCSCCTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1GL26</accession>
    <name>I1GL26_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100842386</name>
        <name evidence="14" type="ORF">BRADI_1g02290v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT73829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GL26"/>
    <dbReference type="EnsemblPlants" id="KQK12226">
        <property type="protein sequence ID" value="KQK12226"/>
        <property type="gene ID" value="BRADI_1g02290v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73829">
        <property type="protein sequence ID" value="PNT73829"/>
        <property type="gene ID" value="BRADI_1g02290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12226">
        <property type="protein sequence ID" value="KQK12226"/>
        <property type="gene ID" value="BRADI_1g02290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73829">
        <property type="protein sequence ID" value="PNT73829"/>
        <property type="gene ID" value="BRADI_1g02290v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQPF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_43_5_1"/>
    <dbReference type="OMA" id="DSHYANN"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GL26">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000704">
        <property type="entry name" value="Os12g0614800 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000708">
        <property type="entry name" value="Protein kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF59">
        <property type="entry name" value="OS12G0615300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093904" description="Protein kinase domain-containing protein" evidence="12">
        <location>
            <begin position="24"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="432"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="PNT73829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="754" mass="83533" checksum="D975DB82CA884AEC" modified="2012-06-13" version="1">MALPVLILSIFFTAVAILTGASSEASISLPGCPNKCGDVLIPYPFGIGEHCAATSLSSYFNLICNSTINPPRPMVGDNDTEVEIADISLEHGEMRVLSPVNHICFTSNTTFTKFVGGYELEPTPFLPSPSRNRFTVIGCNTLGLIGGFKGASSQYVAGCYSFCDGVNNTSDGAPCAGMGCCEAAIPANLTSYGVMFEMNQSKVWGFNPCFYAMVAEVGWYNFRQQDLFGILGFINDRAQRGAPIVADWAIRNGSCPEKGKDRPNDYACVSVNSYCIAANNGPGYLCQCSKGYEGNPYLLNGCQDIDECTLRKQDPKYEDLYPCTKGVCHNTPGSYLCKCKRGTRPDGTNYGCKSRYSPADKLVIGLSVSATVVMALACILLMQFQRKRHKREKDEYFKQNGGLKLYDEMRSRQVDTIRILTEKQIKRATDNYDEDRIIGRGGHGMVYRGTLDDQKEAAIKKSKVISEDWREEFVNEIIILSQINHRNIVRLLGCCLDVDVPMLVYEFVPGGTLSEFLHGADHISPIPLDLRLKMATQSAEALAYLHSSTSRTIIHGDVKSANILLDDQLDAKVADFGASALKSMDETEFIMFVHGTLGYLDPECFISHHLTDKSDVYSFGVVLVELMTRKRAIYTDNFNGKESLSFSFPLMFHQKRHQIMLDLDIIDDAVMVVLEDMAELAVHCLSPRGCDRPTMKEVAERLQVMRRLQLHATSAHENKDYAHHYGGSLWVADPLEETTHGSIGMSELVEELAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1GL44</accession>
    <name>I1GL44_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="20">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC100832620</name>
        <name evidence="26" type="ORF">BRADI_1g02440v3</name>
    </gene>
    <organism evidence="27">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">Bd21</strain>
            <strain evidence="27">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">Bd21</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="20"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562797.1">
        <property type="nucleotide sequence ID" value="XM_003562749.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GL44"/>
    <dbReference type="STRING" id="15368.I1GL44"/>
    <dbReference type="EnsemblPlants" id="KQK12244">
        <property type="protein sequence ID" value="KQK12244"/>
        <property type="gene ID" value="BRADI_1g02440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832620"/>
    <dbReference type="Gramene" id="KQK12244">
        <property type="protein sequence ID" value="KQK12244"/>
        <property type="gene ID" value="BRADI_1g02440v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832620"/>
    <dbReference type="eggNOG" id="ENOG502QRWA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_116_2_1"/>
    <dbReference type="OMA" id="QEPYMTM"/>
    <dbReference type="OrthoDB" id="1918782at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000384">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000016">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000025">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF9">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 23" id="KW-0732">Signal</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093925" description="Receptor-like serine/threonine-protein kinase" evidence="23">
        <location>
            <begin position="30"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="25">
        <location>
            <begin position="23"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="506"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="784"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="838"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="800"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="534"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KQK12244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="862" mass="91892" checksum="20D47109FF6D9C98" modified="2012-06-13" version="1">MRTLWGGEDLAFLLRCCSLLLLLASFSHGADMPTGSSLSPGNQAAPWLSPNSTFSLAFAPSPSSPSLFVAAVTYAGGVPVWSAGAGAAVDSGGSLRLSSTGDLQLVNGSGAVLWSSGTGGRGVAAAALQESGNLVLKNSTGGALWQSFEHPTDTVVMSQSFTSSMNLTSGNYAFAVDRPSGNLTLRWSSSSSGSGGGNAVKYFNKGYNSTFTGNQTLTSPSLVMQSNGIVSLTDTSLSSPAVVAYSSNYGESGDMLRFVRLDADGNFRAYSAARGSSSATEQWSAVVDQCEVFGYCGNMGVCGYNGTSPFCSCPSQNFRPKDAADPRSGCERKVELVNCPGNSTMLELANTQFLTYPPEITTEQFFVGITACRLNCLSGGSCVASTALADGSGLCFLKVSPFVSAYQSASLPSTSFVKVCFPGVPNPPLVAGGGSSGGSSGLRAWVVALVVLGAVSGLVLCEWVLWWVFCRNSPKYGPASAQYALLEYASGAPVQFSYKELQRSTKGFKEKLGAGGFGAVYRGVLANRTVVAVKQLEGIEQGEKQFRMEVATISSTHHLNLVRLIGFCSEGRHRLLVYEFMKNGSLDAFLFAGADAPKMPWSTRFAVAVGTARGITYLHEECRDCIVHCDIKPENILLDEQHNAKVSDFGLAKLINPKDHRHRTLTSVRGTRGYLAPEWLANLPITVKSDVYSYGMVLLEIVSGHRNFDISEETDRKKFSVWAYEEYEKGNIACIVDKRLAEEDIDMAQAERALQVSFWCIQEQPVQRPTMGKVVQMLEGIMELERPPPPKSSDSFLTVTTATGSTSGASSSMASTFASSALVAPMSTPNLEQQMAVGRSASVRDREIASHPLRSSEPYMTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLI9</accession>
    <name>I1GLI9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100842934</name>
        <name evidence="15" type="ORF">BRADI_1g03660v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563584.1">
        <property type="nucleotide sequence ID" value="XM_003563536.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLI9"/>
    <dbReference type="FunCoup" id="I1GLI9">
        <property type="interactions" value="3031"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GLI9"/>
    <dbReference type="EnsemblPlants" id="KQK12423">
        <property type="protein sequence ID" value="KQK12423"/>
        <property type="gene ID" value="BRADI_1g03660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842934"/>
    <dbReference type="Gramene" id="KQK12423">
        <property type="protein sequence ID" value="KQK12423"/>
        <property type="gene ID" value="BRADI_1g03660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842934"/>
    <dbReference type="eggNOG" id="KOG2977">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_9_1_1"/>
    <dbReference type="OMA" id="HMVNTDA"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000115">
        <property type="entry name" value="Dolichyl-phosphate beta-glucosyltransferase isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="86"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK12423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="351" mass="38918" checksum="361A33B779B2A702" modified="2012-06-13" version="1">MAGAAWTLSSAADLLPASLSLSLLLASLVAILVLGVAAVLFDHLRKIGCMHSLERTSVSDAFFEDPSSLNKVPCPSIFDPAEKYISLIVPAYNEECRLPEALTETFNYLKQRSTADKSFSYEVLIVDDGSTDCTSKVAFGYVKKHKIDNVRVLLLGRNHGKGEAVRKGMLHSRGELLLMLDADGATKITDLEKLEAQVRALAKKADSSLAPSATSSQRLSDVEIAVFGSRAHLEKEALATRKWYRNFLMKGFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKHLRIPMAEVSVSWTEIPGSKVRMTSILHMVFELLLIKVGYGLGIWKIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMT6</accession>
    <name>I1GMT6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100835359</name>
        <name evidence="19" type="ORF">BRADI_1g07160v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 15">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558668.1">
        <property type="nucleotide sequence ID" value="XM_003558620.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMT6"/>
    <dbReference type="SMR" id="I1GMT6"/>
    <dbReference type="STRING" id="15368.I1GMT6"/>
    <dbReference type="EnsemblPlants" id="KQK12974">
        <property type="protein sequence ID" value="KQK12974"/>
        <property type="gene ID" value="BRADI_1g07160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835359"/>
    <dbReference type="Gramene" id="KQK12974">
        <property type="protein sequence ID" value="KQK12974"/>
        <property type="gene ID" value="BRADI_1g07160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835359"/>
    <dbReference type="eggNOG" id="KOG1376">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_1_1_1"/>
    <dbReference type="OMA" id="WRDISAC"/>
    <dbReference type="OrthoDB" id="1853138at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000004">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="17">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="18">
        <location>
            <begin position="248"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQK12974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="451" mass="49760" checksum="BFDDD43226368541" modified="2012-06-13" version="1">MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQTSGDKTIGGGDDAFNTFFSETGAGKYVPRAVFVDLEPTVIDEVRTSAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTTSLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVSEITNSAFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVVEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGGDDEDGEEDDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNW0</accession>
    <name>I1GNW0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100839770</name>
        <name evidence="5" type="ORF">BRADI_1g10360v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560489.1">
        <property type="nucleotide sequence ID" value="XM_003560441.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNW0"/>
    <dbReference type="STRING" id="15368.I1GNW0"/>
    <dbReference type="EnsemblPlants" id="KQK13471">
        <property type="protein sequence ID" value="KQK13471"/>
        <property type="gene ID" value="BRADI_1g10360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839770"/>
    <dbReference type="Gramene" id="KQK13471">
        <property type="protein sequence ID" value="KQK13471"/>
        <property type="gene ID" value="BRADI_1g10360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839770"/>
    <dbReference type="eggNOG" id="KOG0385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_17_1_1"/>
    <dbReference type="OMA" id="WNICRID"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GNW0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140750">
        <property type="term" value="F:nucleosome array spacer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000030">
        <property type="entry name" value="ATP-dependent DNA helicase DDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001184">
        <property type="entry name" value="Chromatin complex subunit A 106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="273"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="588"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK13471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="824" mass="92644" checksum="FA292CD8B101D7BB" modified="2012-06-13" version="1">MLEEMVVAVENGVKMESAAAATTDADADSPISVLEDEKMSESKDEKASRFVDASTSLPVKFEANGGEVHPIVQALNAEEALLQSVKVETADEYLDASTSLPIDLEAKNGDASFITEVMSKEEEQLYQARLKEEEEEEEAKTKEAAKHAFDPKARFSKLDELLTQTQLFSEFLLEKMEQITDKGVEVKDEEEPVEEPQKGRGRKRKANSKPQYNDKKAKTAVAAMLTRSQEDRSADDGILTEEEKCEIEQAKLVPLMTGGKLKSYQIKGVKWLISLWQNGLNGILADQMGLGKTIQTIGFLAHLKGNGLHGPYMVIAPLSTLSNWLNEISRFVPSLTGLIYHGDKVTRAELRRKFMPKTAGPDFPIIITSYEIAMSDARKVLSHYKWQYVIVDEGHRLKNSKCKLLSELKHIPMDNKLLLTGTPLQNNLAELWSLLNFILPDIFSSHQEFESWFDFSGKGDEEQQEDTDENKRVVVSKLHAILRPFLLRRMKEDVEQMLPRKKEIIIYANMTEHQRQIQTHLIEKTFDDYLLGSADIVLRPGMKAKLNNLMIQMRKNCAHPDLFNAAFDSTGLYPPIGKLLEQCGKFQLFNRLLDSLLKRKHKVLVFSQWTKVLDLIAYYLDTKGLEVCRIDGGVRLEERRRQIAEFNDLNSSLNVFILSTRAGGLGINLTSADTCILYDSDWNPQADLQAMDRCHRIGQTKPVHVYRLATSNSVEGRIIKKAFGKLKLEHVVIGKGQFQQDRAKPNALDEAELLALLRNEQDEEDRMIQTDISDEDLLKVMDRSDLTGPPGAVDATPLVPLKGPGWEVVVASKSGGGMLTALTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPP5</accession>
    <name>I1GPP5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100842305</name>
        <name evidence="5" type="ORF">BRADI_1g12787v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561423.1">
        <property type="nucleotide sequence ID" value="XM_003561375.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPP5"/>
    <dbReference type="FunCoup" id="I1GPP5">
        <property type="interactions" value="645"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GPP5"/>
    <dbReference type="EnsemblPlants" id="KQK13837">
        <property type="protein sequence ID" value="KQK13837"/>
        <property type="gene ID" value="BRADI_1g12787v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842305"/>
    <dbReference type="Gramene" id="KQK13837">
        <property type="protein sequence ID" value="KQK13837"/>
        <property type="gene ID" value="BRADI_1g12787v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842305"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_28_1_1"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21608">
        <property type="entry name" value="RRM2_NsCP33_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000430">
        <property type="entry name" value="Glycine-rich RNA-binding protein GRP1A"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048289">
        <property type="entry name" value="RRM2_NsCP33-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="6"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="67"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="119"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK13837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="161" mass="15622" checksum="A295AED57A5D75AC" modified="2012-06-13" version="1">MAEVEYRCFVGGLAWATDDRGLQDAFSSFGEIIDSKIINDRETGRSRGFGFVTFASEQSMRDAIEGMNGKDLDGRNITVNEAQSRGGGGGRGGGGGGGYGGRREGGGGGYGGGGGGYGGGGGGGYGQRQGGGGYGGGGGYGGGGGGYGGSRGGSGGGNWRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQD6</accession>
    <name>I1GQD6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SNARE-interacting protein KEULE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100835188</name>
        <name evidence="3" type="ORF">BRADI_1g14940v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562299.1">
        <property type="nucleotide sequence ID" value="XM_003562251.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQD6"/>
    <dbReference type="STRING" id="15368.I1GQD6"/>
    <dbReference type="EnsemblPlants" id="KQK14248">
        <property type="protein sequence ID" value="KQK14248"/>
        <property type="gene ID" value="BRADI_1g14940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835188"/>
    <dbReference type="Gramene" id="KQK14248">
        <property type="protein sequence ID" value="KQK14248"/>
        <property type="gene ID" value="BRADI_1g14940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835188"/>
    <dbReference type="eggNOG" id="KOG1300">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009210_3_0_1"/>
    <dbReference type="OMA" id="PFTRPHT"/>
    <dbReference type="OrthoDB" id="2228at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
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        <property type="entry name" value="Sec-1-like_dom3a"/>
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    </dbReference>
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        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="537"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="549"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK14248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="74866" checksum="84E8CB0A1F6A8522" modified="2012-06-13" version="1">MSLDSDSSSQGGDNRSFRQITRDRLLFEMLRSTRKDSKSAWKVLIMDKFTVKIMSYSCKMADITEEGVSLVEDLYKRRQPLPSLDAIYFIQPTKENIVMFLSDMSGRSPLYKKAFVFFSSPIQKDLVAQIKKDSSVLPRIGALSEMNLEYIPIESQGFVTDHERALEELFCESAEGFNKYNACLNAMATRISTVFASMREFPRVHYRVAKTIDASTMTTLRDMVPTKIAAGVWNYLSKYKTSIPEFPQTETCELLIVDRSVDQIAPIIHEWTYDAMCHDLLCMDGNKYVHEIPSKNGSASEKKEVLLDDHDPVWLELRHAHIADASERLHDKMTHFVSKNKAAQLHQARTGGELSTRDLQKMVQALPQYSDQIDKLSLHVEIAGKLNSIIREQCLRDVGQLEQDLVFGDAGTKELINFLRTQLGVSRENKLRLLMIYAAINPEKFESDKGTKLMQLAGLSADDMIAVNNMRSLCSDTKKPSGGGFTLKFDVHKKKHGYRKERTGEESAWALSRFYPVLEELIEKLSKGELPKDEYNCMNDPSPSFHGLPTTTTSSSARTSPAHSMRSRRTGGSWARPRGSDDGYSSDSVLKHASSDFKKRGQRIFVFVIGGATRSELRAAHKLTSKLKREVILGSSSLDDPPQFITKLKMMSAEELTLDDLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I1GR44</accession>
    <name>I1GR44_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquinol-cytochrome c reductase complex 6.7 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g17665v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GR44"/>
    <dbReference type="FunCoup" id="I1GR44">
        <property type="interactions" value="798"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GR44"/>
    <dbReference type="EnsemblPlants" id="KQK14625">
        <property type="protein sequence ID" value="KQK14625"/>
        <property type="gene ID" value="BRADI_1g17665v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14625">
        <property type="protein sequence ID" value="KQK14625"/>
        <property type="gene ID" value="BRADI_1g17665v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S99R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188810_0_0_1"/>
    <dbReference type="InParanoid" id="I1GR44"/>
    <dbReference type="OMA" id="RPQPTDI"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK14625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="58" mass="6191" checksum="BEC5D8CC8986A315" modified="2012-06-13" version="1">MALPASGLSRLLASRRIQPADISAAATWGVAAGSAAIYLVQPFDWIKKTFFEKPEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1GU61</accession>
    <name>I1GU61_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100833976</name>
        <name evidence="7" type="ORF">BRADI_1g26810v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK16110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="I1GU61">
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    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16110">
        <property type="protein sequence ID" value="KQK16110"/>
        <property type="gene ID" value="BRADI_1g26810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833976"/>
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        <property type="protein sequence ID" value="KQK16110"/>
        <property type="gene ID" value="BRADI_1g26810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833976"/>
    <dbReference type="eggNOG" id="KOG1347">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK16110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="487" mass="52335" checksum="3686A2021B427069" modified="2012-06-13" version="1">MSSAPLLGACERDGKGCCERESPAPAAAGHPWLRRLVDTEEAWAQLQFAVPMVLTNMAYYAIPLVSVMFSGHLGDVHLAGATLGNSWATVTGYAFVTGMSGALETLCGQAYGARLYRMLGLYLQSSLIMSAVVSVAISVLWCYTEPLLLLLHQEPDVSRAAGEFVRYQVPGLFAFSFLQCLLRFLQTQSVVLPLVVCSVAPFAIHVPLNHLLVNVLGLGLAGASVAISVTFWVSCLLLLAYVLRSKEFSETWRGFSADAFKYVLPTIKLATPSAIMVCLEYWAFELLVLIAGLLPNSTVSTSLIAMCSSTEAIAYMITYGFSAAVSTRVSNEIGAGNVEGAKNAVAVTMKLSVFLALSFILLLAFGHSLWASLFSGSAVIVAEFAAITPLMMISIVLDSAQGVLSGVARGCGWQHLAALTNLVAFYFIGMPLAILFAFKLDFYTKGLWSGLICGLTCQSSTLVVITVRTKWSKIVDRMQLEKANYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1GUN4</accession>
    <name>I1GUN4_BRADI</name>
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        <recommendedName>
            <fullName evidence="2">VTT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836843</name>
        <name evidence="3" type="ORF">BRADI_1g28390v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003563103.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GUN4"/>
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        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GUN4"/>
    <dbReference type="EnsemblPlants" id="KQK16362">
        <property type="protein sequence ID" value="KQK16362"/>
        <property type="gene ID" value="BRADI_1g28390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836843"/>
    <dbReference type="Gramene" id="KQK16362">
        <property type="protein sequence ID" value="KQK16362"/>
        <property type="gene ID" value="BRADI_1g28390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836843"/>
    <dbReference type="eggNOG" id="ENOG502QV3G">
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    <dbReference type="HOGENOM" id="CLU_038944_2_2_1"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="expression patterns" value="baseline and differential"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SNARE ASSOCIATED GOLGI PROTEIN FAMILY"/>
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    </dbReference>
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    </dbReference>
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        </location>
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        </location>
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        </location>
    </feature>
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            <end position="275"/>
        </location>
    </feature>
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        <location>
            <begin position="114"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK16362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="289" mass="31035" checksum="6F21E66B34A5C667" modified="2012-06-13" version="1">MGMSGAASGGGAGGGGRGEYMRIPEDVDAIKEAAKEEGDCPRLLRCRAIRWWAKVAVLAIFLAGGAAAAVVFLGPLVIKKVVVPLIDWQSTTFSRPVIALICFGAIALFPSVLLPSSPFMWIAGMTFGYGYGFLIITTAMSIGMSLPFFIGSAFHSRIHRWLEKWPKKAAFVRLAGEGDWFHQFRAVALLRISPFPYLVFNYASVATNVKYCPYIAGSMAGTIHETFLAIYSGKLVQSLAAATSQGSFLSVDQIIYNGIGFSVAAVSTAAITIYAKKALQKLQAEDELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I1GUX3</accession>
    <name>I1GUX3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g29030v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GUX3"/>
    <dbReference type="FunCoup" id="I1GUX3">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16497">
        <property type="protein sequence ID" value="KQK16497"/>
        <property type="gene ID" value="BRADI_1g29030v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16497">
        <property type="protein sequence ID" value="KQK16497"/>
        <property type="gene ID" value="BRADI_1g29030v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QR4C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_502860_0_0_1"/>
    <dbReference type="InParanoid" id="I1GUX3"/>
    <dbReference type="OrthoDB" id="681484at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47482:SF5">
        <property type="entry name" value="FAR1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47482">
        <property type="entry name" value="OS11G0632001 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="288"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="495"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="518"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="529"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK16497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="542" mass="61927" checksum="30AF1138CA999E40" modified="2012-06-13" version="1">MEQFIPHHLWKVLTKSGARLGHDSGVSASSCRYRRKKFEIGPSTRPGYLAELALLRIHLEAYEYFNLYSWEIGFGIRYGRSHNYKSKSGPRKDVIYRTMQNIVYGCEGFPTKKNTSSIRCACGTFIRLLRSDDHGWYIKDCKLEHNHLLSRNIGEKLCWPSHKHIDRYTRELVRNLRQNNVPLTKVYIILGKYFGSTDDVPFNKRSLENFCKQLSKEEANDDGGVVLKTGWPIEKHAATIYTSAMLEMFSDHIFDSAAYNYIEVVPNKKYLTVHSDASRRQKWSKVHYEITISDDGGHYQCECGLAEHMGMLCCHAIRVMMRLGIDKVPDSHILKRWTKEASDVLPHHLAHLQKDRGSLRSQSYRHASLHVAALELATIGDKNIECYHQVIKYMSDGKDKFNEMCQHTDGRSLQEIIAQTELPSPAPADSGQLHVGVIAPGDFCMNNYICSDVPILSCNRLDYDKFMLMEYEFEADALRHMDAPNFVNLQEEVADNGMKLPSGNEPQRCTEGKDEEGTQLSDDGSSTERSTKVGAAERRKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1GV78</accession>
    <name>I1GV78_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein LAZ1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100841312</name>
        <name evidence="7" type="ORF">BRADI_1g29840v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239561.1">
        <property type="nucleotide sequence ID" value="XM_010241259.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311170.1">
        <property type="nucleotide sequence ID" value="XM_024455402.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311171.1">
        <property type="nucleotide sequence ID" value="XM_024455403.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311172.1">
        <property type="nucleotide sequence ID" value="XM_024455404.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311175.1">
        <property type="nucleotide sequence ID" value="XM_024455407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GV78"/>
    <dbReference type="STRING" id="15368.I1GV78"/>
    <dbReference type="EnsemblPlants" id="KQK16665">
        <property type="protein sequence ID" value="KQK16665"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16666">
        <property type="protein sequence ID" value="KQK16666"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16667">
        <property type="protein sequence ID" value="KQK16667"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16668">
        <property type="protein sequence ID" value="KQK16668"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16669">
        <property type="protein sequence ID" value="KQK16669"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT75307">
        <property type="protein sequence ID" value="PNT75307"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT75308">
        <property type="protein sequence ID" value="PNT75308"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841312"/>
    <dbReference type="Gramene" id="KQK16665">
        <property type="protein sequence ID" value="KQK16665"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16666">
        <property type="protein sequence ID" value="KQK16666"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16667">
        <property type="protein sequence ID" value="KQK16667"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16668">
        <property type="protein sequence ID" value="KQK16668"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16669">
        <property type="protein sequence ID" value="KQK16669"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75307">
        <property type="protein sequence ID" value="PNT75307"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75308">
        <property type="protein sequence ID" value="PNT75308"/>
        <property type="gene ID" value="BRADI_1g29840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841312"/>
    <dbReference type="eggNOG" id="KOG2641">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012923_0_0_1"/>
    <dbReference type="OMA" id="IALLYNW"/>
    <dbReference type="OrthoDB" id="5348404at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900458">
        <property type="term" value="P:negative regulation of brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098876">
        <property type="term" value="P:vesicle-mediated transport to the plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="422"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK16669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="53766" checksum="83E5005D3A9682FD" modified="2012-06-13" version="1">MELAEQLLSALRSYAPPLWATLTAGIFVVISLTLSLFLLFNHLSAYKNPEEQKFLVGVILMVPFYAIESYISLVNAPISVDIEILRDGYEAFAMYCFGRYLVACLGGEDRTIEFLKKEGSSGSDAPLLGHASEQRYVNHPFPMNYMLNPWPIGEWFYVIVKFGLVQYMIIKTICAILAVILESFGVYCEGEFKWNCGYSYTAMALNFSQSWALYCLVQFYTAIKDELAHIKPLAKFLTFKSIVFLTWWQGVAIALLSSWGLLRGPIAQELQFKSSIQDFIICIEMGVASVIHLYVFPAKPYELMGDLFTGEVSVLGDYASVDCPLDPDEVKDSERPTKIRLPQPDDNVRCSTGIKESVRDVVLGGGEYIVNDLKFTVNHAVEPINEKIHRISQNMKKHEKEKKQTNDDSCIDSPRSLHRVISGIDDPLLNGSLSDNSGPKKARRHRRRSGTESGGESSDHHGGLGGYEIRGHRWITRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1GY34</accession>
    <name>I1GY34_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100840986</name>
        <name evidence="10" type="ORF">BRADI_1g38160v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560673.2">
        <property type="nucleotide sequence ID" value="XM_003560625.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GY34"/>
    <dbReference type="SMR" id="I1GY34"/>
    <dbReference type="FunCoup" id="I1GY34">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GY34"/>
    <dbReference type="EnsemblPlants" id="KQK18035">
        <property type="protein sequence ID" value="KQK18035"/>
        <property type="gene ID" value="BRADI_1g38160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840986"/>
    <dbReference type="Gramene" id="KQK18035">
        <property type="protein sequence ID" value="KQK18035"/>
        <property type="gene ID" value="BRADI_1g38160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840986"/>
    <dbReference type="eggNOG" id="KOG0223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_3_1_1"/>
    <dbReference type="OMA" id="WRRAPAY"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45724:SF8">
        <property type="entry name" value="AQUAPORIN NIP1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45724">
        <property type="entry name" value="AQUAPORIN NIP2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK18035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="282" mass="30096" checksum="1DA117F5A6976663" modified="2012-06-13" version="1">MARREDDSYTNGSVSMNDFSVEDGRKEKEVYDHDEPEQDGLCGMPVSVPFLQMLLAEFFSTYFLLFAGMGAIVVNNEKDGALTFPGITMVWGLAVMVMIYTVGHISGAHMNPAVSLGFAIAGRMPWKRVPAYMLVQVFAAIIVSVVLRLMFGGRHEFVPVTAPTGSNIQSLVTEFTTTFYLVFVVMAVATDDRAVGSMAGVAVGATITLNALFSGPVTGASMNPARSIGPALVGGKYTSLWVYILGPFAGGAAGAWAYNLMRYTDKPAAVLSDVAKSTDRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZI2</accession>
    <name>I1GZI2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100831623</name>
        <name evidence="2" type="ORF">BRADI_1g45050v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564041.1">
        <property type="nucleotide sequence ID" value="XM_003563993.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZI2"/>
    <dbReference type="STRING" id="15368.I1GZI2"/>
    <dbReference type="EnsemblPlants" id="KQK18837">
        <property type="protein sequence ID" value="KQK18837"/>
        <property type="gene ID" value="BRADI_1g45050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831623"/>
    <dbReference type="Gramene" id="KQK18837">
        <property type="protein sequence ID" value="KQK18837"/>
        <property type="gene ID" value="BRADI_1g45050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831623"/>
    <dbReference type="eggNOG" id="ENOG502RRDG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059651_0_0_1"/>
    <dbReference type="OMA" id="EETCYPC"/>
    <dbReference type="OrthoDB" id="783250at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038943">
        <property type="entry name" value="PLDrp1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33971">
        <property type="entry name" value="OS06G0232000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33971:SF15">
        <property type="entry name" value="OS06G0232000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="384"/>
        </location>
    </feature>
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        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK18837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="384" mass="42510" checksum="3269EA31DE18010E" modified="2012-06-13" version="1">MATAYYGSSRSRDAPTDEPDDFDEFDSTPYGGGYDLFVTFGRPLPPSDETCYPCSAPSTSYDAPHYASEDPSPYAHHQKPQPAYGFRPQQEQKQQHQQPSYGDDSGYGSKPQAAYGFRPQEEEQQSYGSGYGSKPQPAYGFRPQQEEEQSYGSGYGSKPQRTEEDTYGSGYGRKPQEEVSYGSGYGSKPQAEESYGSGYGTRPQQEESYGSGYGSKPQVEQSYGSEYGSGYGRKPQGEESYGSGYGNRPQGGEEYGSGGYGGRKKQEDSYGSSEHGYGRKTEDDSYGGSGYGYGKKAQVEDEGAYGSGYQKPKPYGEETQGSYGYGEEKPRYQSGGYEKPSYGGGEEYQGSHGRKKHDDDDDSDDEKKKRYEKHHNRRHHDYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H1K0</accession>
    <name>I1H1K0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100846131</name>
        <name evidence="7" type="ORF">BRADI_1g50832v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557183.1">
        <property type="nucleotide sequence ID" value="XM_003557135.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H1K0"/>
    <dbReference type="STRING" id="15368.I1H1K0"/>
    <dbReference type="EnsemblPlants" id="KQK19850">
        <property type="protein sequence ID" value="KQK19850"/>
        <property type="gene ID" value="BRADI_1g50832v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846131"/>
    <dbReference type="Gramene" id="KQK19850">
        <property type="protein sequence ID" value="KQK19850"/>
        <property type="gene ID" value="BRADI_1g50832v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846131"/>
    <dbReference type="eggNOG" id="KOG1252">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_1_1"/>
    <dbReference type="OMA" id="ERYIICR"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H1K0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000043">
        <property type="entry name" value="Cysteine synthase, chloroplastic/chromoplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="6">
        <location>
            <begin position="34"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="208"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK19850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="347" mass="37088" checksum="A733F03C29F91770" modified="2012-06-13" version="1">MAPGKEELGTARRGVPSLLLSDGGIGQEHIASDVTQLIGWTPLIELKRIGAKEGLDARIVGKMEAYQPLCSVKDRSALRMIEDAEEKGLISPGVTTLVEPTSGNLGLGLVLICVSKGYRFVAVMPGQYSLDKQILLRYMGAELFITDPALGFPGITDKVEQLKKELPNVHVLDQFVNPANPDAHVRWTGPEIWKDTAGKVDIFVAGSGSGGTVSGVGKYLKMQNPNVKIICVEPTESPVISGGESGKHKIQGIGPGFLPEVLNTSVIDETVTVTTDEAMANARRLAMEEGLLVGISSGANFAACLKVAAREENKGKMIVTMFPSGGERYMNSDLFAAVREECLAMTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2A7</accession>
    <name>I1H2A7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Guanosine nucleotide diphosphate dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100834130</name>
        <name evidence="3" type="ORF">BRADI_1g53020v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557314.1">
        <property type="nucleotide sequence ID" value="XM_003557266.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2A7"/>
    <dbReference type="FunCoup" id="I1H2A7">
        <property type="interactions" value="1990"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H2A7"/>
    <dbReference type="EnsemblPlants" id="KQK20184">
        <property type="protein sequence ID" value="KQK20184"/>
        <property type="gene ID" value="BRADI_1g53020v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834130"/>
    <dbReference type="Gramene" id="KQK20184">
        <property type="protein sequence ID" value="KQK20184"/>
        <property type="gene ID" value="BRADI_1g53020v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834130"/>
    <dbReference type="eggNOG" id="KOG1439">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021695_0_0_1"/>
    <dbReference type="OMA" id="PNTADSH"/>
    <dbReference type="OrthoDB" id="9446342at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H2A7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000010">
        <property type="entry name" value="Guanosine nucleotide diphosphate dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000587">
        <property type="entry name" value="Guanosine nucleotide diphosphate dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF11">
        <property type="entry name" value="GUANOSINE NUCLEOTIDE DIPHOSPHATE DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK20184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="446" mass="49324" checksum="521C10195B5F9F62" modified="2012-06-13" version="1">MDVEYDVIVLGTGLKECILSGLLSVDRLKVLHMDRNDYYGGDSTSLNLNQLWKRFKGEGTPPAHIGASRDYNVDMVPKFMMANGALVRVLIHTGVTKYLSFKAVDGSYVFNKGKIHKVPSTDMEALKSPLMGLFEKRRAGKFFLYVQDYKENDPSTHKGLDLTKMTSKELISKYGLDDNTIDFIGHAVALHKDDSYLSEPAIEIVKRMKLYAESVARFQGGSPYIYPLYGLGELPQGFARLSAVYGGTYMLNKPECKVEFDDEGKVCGVTSEGETAKCRKVVCDPSYVPEKVKKVGKVFRAIAIMSHPIPNTAESHSVQIIIPQKQLGRKSDMYVFCCSYSHNVASKGKFIAFVSAQAESDNPEAELKPGIDLLGPVDELFIDIYDRSEPANDPSSDNCFISTSYDATTHFESTVMDVLSLYTKITGKTVDLSVDLSAASAAEDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1H3P1</accession>
    <name>I1H3P1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_1g57280v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the plant thionin (TC 1.C.44) family. 4 C-C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H3P1"/>
    <dbReference type="FunCoup" id="I1H3P1">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK20880">
        <property type="protein sequence ID" value="KQK20880"/>
        <property type="gene ID" value="BRADI_1g57280v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20880">
        <property type="protein sequence ID" value="KQK20880"/>
        <property type="gene ID" value="BRADI_1g57280v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SUEZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132328_0_0_1"/>
    <dbReference type="InParanoid" id="I1H3P1"/>
    <dbReference type="OMA" id="ADYPKMH"/>
    <dbReference type="OrthoDB" id="653285at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1350.10:FF:000001">
        <property type="entry name" value="Hellethionin-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1350.10">
        <property type="entry name" value="Thionin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001010">
        <property type="entry name" value="Thionin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036391">
        <property type="entry name" value="Thionin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33920">
        <property type="entry name" value="THIONIN-2.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33920:SF2">
        <property type="entry name" value="THIONIN-2.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00321">
        <property type="entry name" value="Thionin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00287">
        <property type="entry name" value="THIONIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57429">
        <property type="entry name" value="Crambin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00271">
        <property type="entry name" value="THIONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0800">Toxin</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094283" evidence="9">
        <location>
            <begin position="29"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK20880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="135" mass="14212" checksum="CD331D2B20A8B274" modified="2012-06-13" version="1">MASNNGLKSVIICILILGLVLEQVQVEGKSCCKNTSSRLCYNACRKAGGSQGACASTCGCIHIDGKRCPADYPKMHLLPDSRESDAIKYCNIGCSSTVCDNMNHVFRGEDNVELCLDACVSLCNGNDSAVASVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1H7L4</accession>
    <name>I1H7L4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100827008</name>
        <name evidence="9" type="ORF">BRADI_1g68560v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_010230593.3"/>
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        <property type="nucleotide sequence ID" value="XM_010230594.3"/>
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        <property type="nucleotide sequence ID" value="XM_010230595.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_014897579.2"/>
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    <dbReference type="EnsemblPlants" id="KQK22645">
        <property type="protein sequence ID" value="KQK22645"/>
        <property type="gene ID" value="BRADI_1g68560v3"/>
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    <dbReference type="EnsemblPlants" id="KQK22646">
        <property type="protein sequence ID" value="KQK22646"/>
        <property type="gene ID" value="BRADI_1g68560v3"/>
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    <dbReference type="EnsemblPlants" id="KQK22647">
        <property type="protein sequence ID" value="KQK22647"/>
        <property type="gene ID" value="BRADI_1g68560v3"/>
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    <dbReference type="GeneID" id="100827008"/>
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        <property type="gene ID" value="BRADI_1g68560v3"/>
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        <property type="gene ID" value="BRADI_1g68560v3"/>
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    <dbReference type="Gramene" id="KQK22647">
        <property type="protein sequence ID" value="KQK22647"/>
        <property type="gene ID" value="BRADI_1g68560v3"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="F:DNA-binding transcription factor activity"/>
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        <property type="term" value="F:sequence-specific DNA binding"/>
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        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="entry name" value="bZIP_plant_GBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bZIP"/>
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    <dbReference type="InterPro" id="IPR045314">
        <property type="entry name" value="bZIP_plant_GBF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044827">
        <property type="entry name" value="GBF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012900">
        <property type="entry name" value="MFMR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45967">
        <property type="entry name" value="G-BOX-BINDING FACTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45967:SF15">
        <property type="entry name" value="OS03G0239400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07777">
        <property type="entry name" value="MFMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16596">
        <property type="entry name" value="MFMR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="302"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="89"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="268"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="355"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="276"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
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            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK22646.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK22645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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    <sequence length="384" mass="40974" checksum="24899618A9CDAD91" modified="2012-06-13" version="1">MGKGDGTTRSKSQKSSATQNEQSTPTNPPTAYPDWSQFQAYYNVPGTAPMTPPAFYHSAVAPSPQGHPYMWGPQMMPPYGTPPPYATMYAQGTPYQQAPMPPGSHPYSPYPVQASNGTVQTPPSGAGGSETDKSSKNKRKTPLKRSKGSLGSLDVVTVKNKMSPAKPLASSSNEGSSQSESGSGSYSEGSSTNSKSGSRTKDEHGQGNDASNKGATASSAVEPTQVSSGPVVLNPMMPYWPVPPPMAGPAGPATGVNMGMDYWGAPTSVPMHGKVAAAPTSAPSSNSRDIILSDPAIKDEREVKRQKRKQSNRESARRSRLRKQAEWEEVANRADLLKQENSSLKEELKRLQEKCDSLTSENTSLHEKLKELDGEKSNGNSCKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1H7R4</accession>
    <name>I1H7R4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100836494</name>
        <name evidence="18" type="ORF">BRADI_1g69000v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cytoplasmic vesicle</location>
            <location evidence="13">COPI-coated vesicle membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the COPG family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK22718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003558375.4"/>
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        <property type="protein sequence ID" value="KQK22718"/>
        <property type="gene ID" value="BRADI_1g69000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836494"/>
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        <property type="protein sequence ID" value="KQK22718"/>
        <property type="gene ID" value="BRADI_1g69000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836494"/>
    <dbReference type="eggNOG" id="KOG1078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OMA" id="YASQVMR"/>
    <dbReference type="OrthoDB" id="1074925at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H7R4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
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        <property type="term" value="C:Golgi membrane"/>
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        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000078">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1480:FF:000002">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000011">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1480">
        <property type="entry name" value="Coatomer, gamma subunit, appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032154">
        <property type="entry name" value="Coatomer_g_Cpla"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037067">
        <property type="entry name" value="Coatomer_gsu_app_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF5">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16381">
        <property type="entry name" value="Coatomer_g_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="15">
        <location>
            <begin position="39"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer gamma subunit appendage Ig-like subdomain" evidence="16">
        <location>
            <begin position="632"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer subunit gamma C-terminal" evidence="17">
        <location>
            <begin position="780"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="615"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK22718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="897" mass="99809" checksum="98904F8313C7A1B0" modified="2012-06-13" version="1">MPRSLAGDLTDPEMAQPYMKKDDDESEIEYSPFFGIEKGAVLQEARAFHDPQLDARRCSQVITKLLYLINQGETFTKVEATEVFFAVTKLFQSNDAGLRRMVYLIIKELSPSSDEVFIVTSSLMKDMNSKTDMYRANAIRVLCRIIDGNLLTQIERYLKQAIVDKNPVVSSAALVSGIHLLQANPEIVKRWSNEVQEAVQSRAALVQFHGLALLHQTRQNDRLAVSKLVSSLTRGSVRSPLAQCLLIRYTSQVIRESSTNTQNGDRPFFDYLESCLRHKAEMVILEAARKITEMDVTSRELAPAITVLQLFLSSSKPVLRFAAVRTLNKVAITRPLAVTNCNVDLETLMSDQNRSIATLAITTLLKTGNESSVDRLMKQITSFMSDIADEFKIVVVEAIRSLCLKFPLKYRSMMIFLSNSLREEGGFEYKKAIVDAIVTLISAIPDAKEIGLLHLCEFIEDCEFTYLSSQILHFLGNEGPRTSDPSRYIRYIYNRVILENSTVRASAVSTLAKFGALVEGLKPRIFVLLRRCLFDTDDEVRDRATLYLQTLNGEVAVGNTEKDVNEFLFGSFDVPLANLEASLRTYEPSEEPFDISLVSREAKSQPLQDKKAPGKKPPAGVPPPAPVSAVDAYQKMLSSIAEFSGFGRLFKSSEPVELTEAETEYAVNVVKHIYESHVVLQYNCTNTIPEQLLQDVTVYVDATDAEEFSEVCLKSLKTLAYDSPGQIFVAFEKPGRVPATCKFSNVLKFTVKEVDTSSGEVDDDGVEDEYQIEDLEIVSADYMLRVAVSNFRNAWENMDPESERVDEYGLGVRESLAEAVSAVTSILGMQPCEGTEVVPSNARSHVCLLSGVFIGDVKVLVRLSFGLSGPKEVAMKLAVRSDDPEVSDKIHEIVASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAN6</accession>
    <name>I1HAN6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100832693</name>
        <name evidence="4" type="ORF">BRADI_1g77880v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559001.1">
        <property type="nucleotide sequence ID" value="XM_003558953.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAN6"/>
    <dbReference type="STRING" id="15368.I1HAN6"/>
    <dbReference type="EnsemblPlants" id="KQK24055">
        <property type="protein sequence ID" value="KQK24055"/>
        <property type="gene ID" value="BRADI_1g77880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832693"/>
    <dbReference type="Gramene" id="KQK24055">
        <property type="protein sequence ID" value="KQK24055"/>
        <property type="gene ID" value="BRADI_1g77880v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832693"/>
    <dbReference type="eggNOG" id="ENOG502S4UK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124154_0_0_1"/>
    <dbReference type="OMA" id="LFWPNRF"/>
    <dbReference type="OrthoDB" id="769005at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35107">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35107:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094420" evidence="3">
        <location>
            <begin position="23"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK24055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="179" mass="18810" checksum="613398B6B400107B" modified="2012-06-13" version="1">MAAARLLLVLAAAATLLAAASARPCGHAQTLLISFSSVSRPNPDPANPSPLTTTVVTVLRVRRLGPHRHPHLQIHRPAEPLPKSLPGAAAAQPSSFQERAKDILVVVSGLLFGFGCGALTAASMYLVWSLLASIGGASYEEVYSDDEDDEEEVSDGAESPKKAGYVIIHDTEEYAAGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HGW5</accession>
    <name>I1HGW5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100826843</name>
        <name evidence="3" type="ORF">BRADI_2g17820v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565967.1">
        <property type="nucleotide sequence ID" value="XM_003565919.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HGW5"/>
    <dbReference type="STRING" id="15368.I1HGW5"/>
    <dbReference type="EnsemblPlants" id="KQK05073">
        <property type="protein sequence ID" value="KQK05073"/>
        <property type="gene ID" value="BRADI_2g17820v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826843"/>
    <dbReference type="Gramene" id="KQK05073">
        <property type="protein sequence ID" value="KQK05073"/>
        <property type="gene ID" value="BRADI_2g17820v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826843"/>
    <dbReference type="eggNOG" id="KOG0198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="OMA" id="CHINSSM"/>
    <dbReference type="OrthoDB" id="663499at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000466">
        <property type="entry name" value="MAP kinase kinase kinase18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052751">
        <property type="entry name" value="Plant_MAPKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011:SF39">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK05073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="475" mass="50303" checksum="FCD915A17C0B1E8C" modified="2012-06-13" version="1">MAMAACSGPWKRVRTLGRGASGAEVFLAADDVSGELFAVKSAGAGATLRREQEIMAGLRSPNVLSCIGGRVGHDGSYQLFLEFAPGGSLADAVRSGAGGRLEERVVRAYAEDMAAGLAYLHGAGLVHGDVKPRNVVIGGDGRAKLADFGCSRKTDSRGPILGGTPAFMAPEVARGEEQGPAADIWALGCTVVEMATGRAPWNGTGMEDDGVLAALHRIGYTDDAVPEVPKWLSADAKDFLARCLTRRPSDRCTAAQLLEHPFLSAEREWVSPKSTLDAAFWETDGSEEEDDDAEVSAVEHSMAERIRALACSASSLPEDWDSDEGWIDVMNSTPSEASDAAPVPVPAEETTEPDDGGVRSEEWSAETSGLGLGITVDSSVTARDDVEEADNNSLISHNRILQSSEISVFSGAGRDDVGEADDDSIGHSRILQSLEISVLADEAVPCKLLCHINSSMDFTPQIFPRPLPRCLRFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HK46</accession>
    <name>I1HK46_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100823368</name>
        <name evidence="6" type="ORF">BRADI_2g27440v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566320.1">
        <property type="nucleotide sequence ID" value="XM_003566272.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231487.1">
        <property type="nucleotide sequence ID" value="XM_010233185.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231488.1">
        <property type="nucleotide sequence ID" value="XM_010233186.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231489.1">
        <property type="nucleotide sequence ID" value="XM_010233187.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231490.1">
        <property type="nucleotide sequence ID" value="XM_010233188.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315171.1">
        <property type="nucleotide sequence ID" value="XM_024459403.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315172.1">
        <property type="nucleotide sequence ID" value="XM_024459404.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315173.1">
        <property type="nucleotide sequence ID" value="XM_024459405.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315174.1">
        <property type="nucleotide sequence ID" value="XM_024459406.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315175.1">
        <property type="nucleotide sequence ID" value="XM_024459407.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315176.1">
        <property type="nucleotide sequence ID" value="XM_024459408.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315177.1">
        <property type="nucleotide sequence ID" value="XM_024459409.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315178.1">
        <property type="nucleotide sequence ID" value="XM_024459410.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315179.1">
        <property type="nucleotide sequence ID" value="XM_024459411.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315180.1">
        <property type="nucleotide sequence ID" value="XM_024459412.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315181.1">
        <property type="nucleotide sequence ID" value="XM_024459413.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315182.1">
        <property type="nucleotide sequence ID" value="XM_024459414.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315183.1">
        <property type="nucleotide sequence ID" value="XM_024459415.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315184.1">
        <property type="nucleotide sequence ID" value="XM_024459416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HK46"/>
    <dbReference type="EnsemblPlants" id="KQK06649">
        <property type="protein sequence ID" value="KQK06649"/>
        <property type="gene ID" value="BRADI_2g27440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823368"/>
    <dbReference type="Gramene" id="KQK06649">
        <property type="protein sequence ID" value="KQK06649"/>
        <property type="gene ID" value="BRADI_2g27440v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823368"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_37_8_1"/>
    <dbReference type="OMA" id="DSCVWGA"/>
    <dbReference type="OrthoDB" id="428658at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003129">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000598">
        <property type="entry name" value="pentatricopeptide repeat-containing protein At1g20230 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000515">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000607">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF386">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="82"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="117"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="218"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="253"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="354"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="389"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="569"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK06649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="661" mass="72313" checksum="6F47E206747385CC" modified="2012-06-13" version="1">MSHSNSLYHFLRHVSFPPDPHLLPSALKSCPAQPLARALHAAAVVSGLAEDPFVASSLLHSYIRLGATGAARSVFDRMPEKNVVGWSALIAGYSARGDAEAAWGLLEQMRSAGVEPNVITWNGLVSGLNRSGRALDAVTALVRMHSEGFFPDATGVSCALSAVGDVKEVSVGKQVHGYVVKAGCRLDACVVTALIDMYGKCGRADEIVRVFHESSHMDVASCNALVAGLSRNAQVSEALLLFREFICRGVELNVVSWTSIVACCVQNGRDLEAVDLFRTMQSIGVEPNSVTIPCVLPAFANVAALMHGRSAHCFSLRKGFLHDVYVGSALVDMYAKCGKARHARTIFDAMPSRNVVSWNAMIGGYAMHGDAANAVQLFCSMQKCKQKPDLVTFTCVLGACSQAGLTEEGRRYFNEMQQGHGISPRMEHYACMVTLLGRSGKLDEAYDLINEMPFEPDSCIWGSLLGSCRVYGNVLLAEVAAEKLFQLEPGNAGNYVLLSNIYASKKMWDGVNRVRDEMKNMGLKKEKGCSWIEIKNKVHMLLAGDNSHPMMTAITEKLNQLTIEMNRLGFAPSRDFVLHDVEEQEKDNILAVHSEKLAVALGLISTRPGTPLRVIKNLRICGDCHEAMKFISSFEQREISVRDTNRFHHFKDGKCSCGDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HNE8</accession>
    <name>I1HNE8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100822028</name>
        <name evidence="5" type="ORF">BRADI_2g40625v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314669.1">
        <property type="nucleotide sequence ID" value="XM_024458901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HNE8"/>
    <dbReference type="EnsemblPlants" id="KQK08238">
        <property type="protein sequence ID" value="KQK08238"/>
        <property type="gene ID" value="BRADI_2g40625v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822028"/>
    <dbReference type="Gramene" id="KQK08238">
        <property type="protein sequence ID" value="KQK08238"/>
        <property type="gene ID" value="BRADI_2g40625v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_0_1_1"/>
    <dbReference type="OMA" id="GIHGFCL"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900864">
        <property type="term" value="P:mitochondrial RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000351">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000529">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001475">
        <property type="entry name" value="Selenium-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001683">
        <property type="entry name" value="Selenium-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094742" description="Pentacotripeptide-repeat region of PRORP domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="69"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="170"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="271"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="372"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="407"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK08238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="555" mass="61380" checksum="744975DDD4F14810" modified="2012-06-13" version="1">MRRKLALLLAAANGAALPLSHTKQIHARLLLVSPFIPDDVRLFLLRSYAGRGDFASAHCLLDETPRPVSPLLYNAVIRAHARRLDLPAALALFARMRRSATPPDAHTFACVVRACADCSSPDTAKIVHGVASSSGMSSHPVVGSALVSAYSKFLLVDSARHMFDGLPDPDLVLWNSMLSGYGYREMWVEGLQLFSAMRRAGEEPDGYSMVSLVSSFRNPEALDFGQAVHGVCIKGGYDSGHHVRSALVSMYFRCGCMESGQTLFGNLLDADLVTWSSLITGQLQTGKYEESFNLFRQMCYSGRRPDSILIASLLSACASTATVSCSREIHCYAIRIGADADVKVSSSLMDAYAKCGFAELGYLVFRQMPNKNSVVYNMVISNLGSHGFALKAIEVHDEMICDKYRPDGATFSALLAACCHAGLLDEGWKLFRRMQDEFHIVVEMEHYVYMVRLLATFNQLKEAYDLIQTMPMAPDCGVWGALLWGCCFHRNSSLGRVVSEKLFEIYPDKAAYGIMLSNLYASQDMWWDAEEVRSEILKEGMHKNTGISWVGEVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPS5</accession>
    <name>I1HPS5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100832441</name>
        <name evidence="10" type="ORF">BRADI_2g44770v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566926.2">
        <property type="nucleotide sequence ID" value="XM_003566878.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPS5"/>
    <dbReference type="STRING" id="15368.I1HPS5"/>
    <dbReference type="EnsemblPlants" id="KQK08897">
        <property type="protein sequence ID" value="KQK08897"/>
        <property type="gene ID" value="BRADI_2g44770v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832441"/>
    <dbReference type="Gramene" id="KQK08897">
        <property type="protein sequence ID" value="KQK08897"/>
        <property type="gene ID" value="BRADI_2g44770v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832441"/>
    <dbReference type="eggNOG" id="KOG0048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028567_25_8_1"/>
    <dbReference type="OMA" id="FWNAVEN"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000011">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044676">
        <property type="entry name" value="EOBI/EOBII-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675:SF27">
        <property type="entry name" value="OS01G0637800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="64"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="64"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="117"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK08897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="217" mass="24497" checksum="2052923F7C36268E" modified="2012-06-13" version="1">MARMICGRTGEPAVRKGPWTLEEDLILVGYISQHGEGSWDNLARSAGLNRNGKSCRLRWLNYLRPGLRRGSISPEEDMVIRELHTRLGNKWSEIAKHLPGRTDNEVKNYWRTRIHKKPHQSKLQLQAQQHPGLDSVATSESASASASTSQASSTVGDEYMQASFPYPELTWVADQSDVGAITTRFFSSSDDFGDNVWNVEDRFWETLPFADPVYEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HS39</accession>
    <name>I1HS39_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100837476</name>
        <name evidence="4" type="ORF">BRADI_2g51310v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="PNT72944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567161.1">
        <property type="nucleotide sequence ID" value="XM_003567113.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315860.1">
        <property type="nucleotide sequence ID" value="XM_024460092.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315861.1">
        <property type="nucleotide sequence ID" value="XM_024460093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HS39"/>
    <dbReference type="SMR" id="I1HS39"/>
    <dbReference type="STRING" id="15368.I1HS39"/>
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        <property type="protein sequence ID" value="KQK09976"/>
        <property type="gene ID" value="BRADI_2g51310v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72943">
        <property type="protein sequence ID" value="PNT72943"/>
        <property type="gene ID" value="BRADI_2g51310v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72944">
        <property type="protein sequence ID" value="PNT72944"/>
        <property type="gene ID" value="BRADI_2g51310v3"/>
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    <dbReference type="GeneID" id="100837476"/>
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        <property type="protein sequence ID" value="KQK09976"/>
        <property type="gene ID" value="BRADI_2g51310v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_2g51310v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT72944"/>
        <property type="gene ID" value="BRADI_2g51310v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837476"/>
    <dbReference type="eggNOG" id="ENOG502QQDN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058353_3_3_1"/>
    <dbReference type="OMA" id="PVNRRHE"/>
    <dbReference type="OrthoDB" id="1903788at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
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    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529:SF54">
        <property type="entry name" value="LATERAL ORGAN BOUNDARIES DOMAIN PROTEIN 18-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31529">
        <property type="entry name" value="LOB DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="43"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
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        <location>
            <begin position="205"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK09976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="236" mass="25210" checksum="F9F71515BABDEA62" modified="2012-06-13" version="1">MQGAPDQPPCSKHAVTRTAGAAPGQQQQQRAEKEEASSAGPGAPCGACKFLRRRCAPGCVFAPHFSGGGGRERGAAQFAAVHRVFGASNVAKLLSRVPVALRRDTARTVCYEAQARIADPVYGCVGTILALQHQVALLQGQLSILQSQLFNYRLAFASTHPDADHFAALQPAYSAGSAPSQMVTYDDLPQAVDFMDVEPQMRGLESLQLSQPPHLEENESQDMSPFSDNVGQQPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1HTD8</accession>
    <name>I1HTD8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100823746</name>
        <name evidence="11" type="ORF">BRADI_2g54940v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/BELL homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564609.1">
        <property type="nucleotide sequence ID" value="XM_003564561.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HTD8"/>
    <dbReference type="FunCoup" id="I1HTD8">
        <property type="interactions" value="1284"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HTD8"/>
    <dbReference type="EnsemblPlants" id="KQK10570">
        <property type="protein sequence ID" value="KQK10570"/>
        <property type="gene ID" value="BRADI_2g54940v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73195">
        <property type="protein sequence ID" value="PNT73195"/>
        <property type="gene ID" value="BRADI_2g54940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823746"/>
    <dbReference type="Gramene" id="KQK10570">
        <property type="protein sequence ID" value="KQK10570"/>
        <property type="gene ID" value="BRADI_2g54940v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT73195"/>
        <property type="gene ID" value="BRADI_2g54940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823746"/>
    <dbReference type="eggNOG" id="KOG0773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011058_3_0_1"/>
    <dbReference type="OMA" id="AGGQMYH"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000117">
        <property type="entry name" value="BEL1-like homeodomain protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006563">
        <property type="entry name" value="POX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07526">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00574">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="371"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="373"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="65"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="449"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK10570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="612" mass="65524" checksum="5A646385739DA0D5" modified="2012-06-13" version="1">MSSPAGGGYGGGAGSGAEPAHHHGHAHGHLLLQHHYAHHVAAAAAAAAGGQMYHVPQHSRREKLRFPPDGAAASDSPATQQLAPQQHGAPGAWPPPAFYSYASSSSSYSPHSPTTLPQQTVPSNGLTAAPQQLPQIPAQQNFSLSLSSASSNPPATPRRQPQQQLAGARGPAAPAAATGPYGPFTGYATVLGRSRFLDPAQKLLEEICDVGGAGAHVDRSVPGEDLLDADPVDVEDHDVVGHELDAATDRDAGSMSGAEQHWKKTRLISMMEEVCKRYRQYYQQVQAVIASFETVAGFSNAAPFAAMALRVMAKHFKCLKSMILNQLRNTSKIAVKEGMSKDIVVFGLGGGGGGGAGFQRGSSVNGFGQPNNIWRPQRGLPERSVSVLRAWLFEHFLHPYPTDGDKQMLAKQTGLTRNQVSNWFINARVRLWKPMVEEIHNLEMKIHKRSAPDKGQHGIHNLTQHSSQCSGKRSEPCDSQPGQISSITRNHHTPASHGFPDELSQMSQSIQQSQVSLAYNRLSSQHNMASPQHQHVSGVGGAGSSSVSLTLGLHQNNRVCFGEPLQSALPANLAHRFGLEDVNDAYAMSSFAGQDRHFTKDIGGHLLHDFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I1HWK6</accession>
    <name>I1HWK6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100845802</name>
        <name evidence="3" type="ORF">BRADI_3g02110v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570840.1">
        <property type="nucleotide sequence ID" value="XM_003570792.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HWK6"/>
    <dbReference type="FunCoup" id="I1HWK6">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92995">
        <property type="protein sequence ID" value="KQJ92995"/>
        <property type="gene ID" value="BRADI_3g02110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845802"/>
    <dbReference type="Gramene" id="KQJ92995">
        <property type="protein sequence ID" value="KQJ92995"/>
        <property type="gene ID" value="BRADI_3g02110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845802"/>
    <dbReference type="eggNOG" id="ENOG502R3B0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030845_0_0_1"/>
    <dbReference type="OMA" id="THHGSEM"/>
    <dbReference type="OrthoDB" id="586448at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046533">
        <property type="entry name" value="DUF6598"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065:SF91">
        <property type="entry name" value="DUF6598 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065">
        <property type="entry name" value="OS07G0486400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20241">
        <property type="entry name" value="DUF6598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6598" evidence="2">
        <location>
            <begin position="169"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="439" mass="49708" checksum="BE4D1F788F73A256" modified="2012-06-13" version="1">MDVDSEEAGRKRETHHGSEMAGGSRRTSKKISETTEWPSVIELMDASAKVSREKYMKRLVAKRNAENPDAAPLVLEELTEQQRTSLKEDDLAREFVLAEAYVASRMEEYSRVEELDPNQDNDDRVYQEDRERWGRKWAKEFGSFEDKTRIPAMCFTDDPMFHITRDLPTMQIFSVKVKEVHEKLQWALDVYGIIAVRDDIDHNRNVIFERTRDNCQTLDEQDPYLVLTGPARAPVMLFEPVRLDVMLKVTCTDKSEDKDLSLLVRRYECCESINYQVSHGELVANSCVGSKRFKSKLSTLELTCGIVVSSVEATISVRIVEGSWPDGFSGRFTASTACVSHMKVLLLNFGDGNVLVSADGTIELSRRVVSVESFGELRICAAAWRGSKQVDREVLFQPLKSGRSSQVLNVGVCKLEITVAWSLFPLSYPTRRIPSSKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>I1HX60</accession>
    <name>I1HX60_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Pathogenesis-related homeodomain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100822239</name>
        <name evidence="13" type="ORF">BRADI_3g03747v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PHD-associated homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570921.1">
        <property type="nucleotide sequence ID" value="XM_003570873.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HX60"/>
    <dbReference type="STRING" id="15368.I1HX60"/>
    <dbReference type="EnsemblPlants" id="KQJ93304">
        <property type="protein sequence ID" value="KQJ93304"/>
        <property type="gene ID" value="BRADI_3g03747v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT65868">
        <property type="protein sequence ID" value="PNT65868"/>
        <property type="gene ID" value="BRADI_3g03747v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT65869">
        <property type="protein sequence ID" value="PNT65869"/>
        <property type="gene ID" value="BRADI_3g03747v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822239"/>
    <dbReference type="Gramene" id="KQJ93304">
        <property type="protein sequence ID" value="KQJ93304"/>
        <property type="gene ID" value="BRADI_3g03747v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65868">
        <property type="protein sequence ID" value="PNT65868"/>
        <property type="gene ID" value="BRADI_3g03747v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65869">
        <property type="protein sequence ID" value="PNT65869"/>
        <property type="gene ID" value="BRADI_3g03747v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822239"/>
    <dbReference type="eggNOG" id="KOG4299">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="EGFCHAN"/>
    <dbReference type="OrthoDB" id="1903104at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HX60">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001103">
        <property type="entry name" value="Homeodomain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000568">
        <property type="entry name" value="Pathogenesis-related homeodomain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12628:SF10">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12628">
        <property type="entry name" value="POLYCOMB-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="11">
        <location>
            <begin position="198"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="443"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="445"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="71"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="284"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="401"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="PNT65869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="736" mass="82009" checksum="3E0DDFAF0BBB2313" modified="2012-06-13" version="1">MNTSENKSACSIPRRSSRRRKQSSSELVSLSKRPSHRNASSSYKLDQCSPKRTARTARNANLAKYIKNKYYRSPLSQRRSSSAVSGKTATGPVRRRSRKRRRQNTDCDEATRLERRARYLLIKIKSEQNLLDAYSGDGWNGHSREKLKPEKELQRAKRQIIKSKIAIRDIIHQLDLYSSSGNMDDSVMPPDEPVNPDNTICSRCKSDESVPDNKIIFCEGSCKMSYHQKCSEPPFDKILPTGGHGWLCKFCLCKMKILEAVNAHLGTSLTVTCPSKDIFKEATEHIGSDDGPGEDLLSEYSGDEDYDPEENDGTSSSLGRGEESISSESNCSGSPLYSPNDDIPGFISADFTDAEGFCHANSHLEFDSGEDVTAEMVNYQRPKRDVDYRRLNEEMFGKLAENEKQSDDEDWGVNRKKRRRVDSAGGAKSVEGVSGVTSNENLQPHRRKLFRMPPAAVEVLRKVFAVDELPARDVKEKLATELGISYEKIDKWFKNTRCAALRDRKAEGNSHIAGPSKSSGKRVEKAEVSGKFDSVDNSYLPPLSKTIETKPESNSRPVRRRLHNKGVSLCPTSEVKETTSPTIKHCSNTHPIHLADRNINTEDRAISQVDTGPSDDPFFDAILAYPNINTSDKVVSTGDLVTLADEPFLDAMLADSHVFYTERTVSREDVRGPSNDQPFLDVIENVCGLKHRLQRLEDMLSSAAPGDTGTAKGDVENQLVVLVPTAELKDKPQPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1C6</accession>
    <name>I1I1C6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100832668</name>
        <name evidence="10" type="ORF">BRADI_3g16260v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573450.1">
        <property type="nucleotide sequence ID" value="XM_003573402.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1C6"/>
    <dbReference type="EnsemblPlants" id="KQJ95285">
        <property type="protein sequence ID" value="KQJ95285"/>
        <property type="gene ID" value="BRADI_3g16260v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832668"/>
    <dbReference type="Gramene" id="KQJ95285">
        <property type="protein sequence ID" value="KQJ95285"/>
        <property type="gene ID" value="BRADI_3g16260v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832668"/>
    <dbReference type="eggNOG" id="KOG1277">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010714_0_2_1"/>
    <dbReference type="OMA" id="ANSVAWY"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF168">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982764" description="Transmembrane 9 superfamily member" evidence="9">
        <location>
            <begin position="23"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="322"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="513"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ95285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="588" mass="66968" checksum="827A99E86E66DFA0" modified="2012-06-13" version="1">MARAIAALAVAVLACVLLGAAADGSDHRYKEGDRVPLYANKVGPFHNPSETYRYYDLPFCAPEHPKDKKEALGEVLNGDRLVDAPYELNFKEDKNSKTLCKKTLSKEEVAKLRDAVAKDYYFQMYYDDLPLWGFLGKLEKDKEQGAGKYLLFKHIHFDIMYNNDRVVEINVQTDPNVAVDITEDKEVPVEFSYSVAWKKTDIPFEKRMEKYSKSSSMPQHLEIHWFSIINSCVTVLLLTGFLATILMRVLKNDFIKYSHEDESLEDQEETGWKYIHGDVFRFPQQKSLFAAIIGSGSQLLALAIFIFLLAIVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYLQLEGTNWVRNLILTGCLFCGPLFLTFSFLNTVAIAYSATAALPFGTIIVIILIWALVTSPLLVLGGIAGKNSNTEFQAPCRTTKYPREIPELPWYRSTIPQMAMAGFLPFSAIYIELYYIFASIWGHKIYTIYSILFIVFIILIIVTAFVTVALTYFQLAVEDHKWWWRSVLCGGSTGIFIFFYCIYYYHARSDMSGFMQTSFFFGYMTCVCYGFFLMLGTVGFRASLLFVRHIYRSIKCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1I4L7</accession>
    <name>I1I4L7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-dependent kinase inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100835341</name>
        <name evidence="6" type="ORF">BRADI_3g28507v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CDI family. ICK/KRP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574044.1">
        <property type="nucleotide sequence ID" value="XM_003573996.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I4L7"/>
    <dbReference type="FunCoup" id="I1I4L7">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I4L7"/>
    <dbReference type="EnsemblPlants" id="KQJ97051">
        <property type="protein sequence ID" value="KQJ97051"/>
        <property type="gene ID" value="BRADI_3g28507v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835341"/>
    <dbReference type="Gramene" id="KQJ97051">
        <property type="protein sequence ID" value="KQJ97051"/>
        <property type="gene ID" value="BRADI_3g28507v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835341"/>
    <dbReference type="eggNOG" id="ENOG502QXA1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083146_0_1_1"/>
    <dbReference type="OMA" id="HTPTSHE"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I4L7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045740">
        <property type="term" value="P:positive regulation of DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="p27"/>
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        <property type="entry name" value="CDI_dom"/>
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        <property type="entry name" value="CDI_dom_sf"/>
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        <property type="entry name" value="KRP"/>
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        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CDK_inhib_pln"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="5">
        <location>
            <begin position="153"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR017811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ97051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="198" mass="22020" checksum="F11FB3E176FA41C4" modified="2012-06-13" version="1">MGKYMRKAKASGEVAVMEVTAAPLGVRTRSRALAMQRLPQEVGKEQGDYLELRGRKLEKLAPPPREPAARKCGGGRRAAAAELVAAEWAEAEPDEVSFGENVLELMAMERNARETTPCSLIRDPETISTPGSTTRLSHSNSHRRVQAPVRHIIPSSAEMNEFFAAAEQLQQQSFIERYNFDLANDCPLPGRYKWVKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1I7K0</accession>
    <name>I1I7K0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100844392</name>
        <name evidence="11" type="ORF">BRADI_3g37370v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574574.1">
        <property type="nucleotide sequence ID" value="XM_003574526.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I7K0"/>
    <dbReference type="STRING" id="15368.I1I7K0"/>
    <dbReference type="EnsemblPlants" id="KQJ98519">
        <property type="protein sequence ID" value="KQJ98519"/>
        <property type="gene ID" value="BRADI_3g37370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844392"/>
    <dbReference type="Gramene" id="KQJ98519">
        <property type="protein sequence ID" value="KQJ98519"/>
        <property type="gene ID" value="BRADI_3g37370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844392"/>
    <dbReference type="eggNOG" id="KOG2604">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011639_1_1_1"/>
    <dbReference type="OMA" id="DEFELWG"/>
    <dbReference type="OrthoDB" id="296793at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I7K0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009860">
        <property type="term" value="P:pollen tube growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048685">
        <property type="entry name" value="COG3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048320">
        <property type="entry name" value="COG3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007265">
        <property type="entry name" value="COG_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302:SF8">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20671">
        <property type="entry name" value="COG3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04136">
        <property type="entry name" value="COG3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 N-terminal" evidence="9">
        <location>
            <begin position="106"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 C-terminal" evidence="10">
        <location>
            <begin position="275"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ98519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="770" mass="86312" checksum="DB3406F32E7D0D51" modified="2012-06-13" version="1">MATTPATLPKSGAVSKGYNFAYAWEKNAPLTEQQNAAISALSHAVAERPFPANLEDGCTAMPEKESALEEAGAMDAVLVNTHQFYKWFAELESAMKSETEEKYRLYESTLEERVKTCDGILQQVDDTLNLFEELQSLHSSVAIKTKTLHDACDQLLVEKQRLIEFAEALRSRLNYFDELENASTSFYSQTMNIGNEQFLPLLKRLDDCISYVENNPQYAESAVYLVKFRQLQSRALGMIRSHVLSTLKAASSQVQAAIRGSDSGKNAVTEGVEASLIYVRFKAAAGELKPIFAEIESRSSKKEYAQILSECHSLFCEQRLYLIRGMVQQRISEFAKKEALPSLTRSGCAYLMEACQFEHQLFAHFFPASAPDVSSMAPLMDPLCSHLYDTLRPRLIYEGNIDSLCELVDILKVEVLGEQLSRRGESVAGLRPILQRILADVHERLAFCARTHIREEIANFRPSDEDLDYPGKLERSATSSANVNDNSDIYAAWYRPLEKTVSCLSRLYHCLESSVFTGLAQEAVEVCSTSLQNASKVITKRATPMDGQLFLIKHLLILREQIAPFDIEFSVTHKELDFSHLLDHLRRILRGQVSLFDWSRSTSLARTFSPRVLENQIDARKELEKSLKSTCEEFIMSITKLVVDPMLSFVTKVTAVKVALSSGSQGQKLDSVLAKPLKTQAFASPDKVAELVQKVGTSIQQDLPKVMTKMTLYLQNPSTRLILFKPIKTNIVEAHIQLQSLLKSEYSADEIQSIGMLSISDLQSQLDGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8P9</accession>
    <name>I1I8P9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CREG-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100823180</name>
        <name evidence="3" type="ORF">BRADI_3g40620v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235319.1">
        <property type="nucleotide sequence ID" value="XM_010237017.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8P9"/>
    <dbReference type="EnsemblPlants" id="KQJ99029">
        <property type="protein sequence ID" value="KQJ99029"/>
        <property type="gene ID" value="BRADI_3g40620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823180"/>
    <dbReference type="Gramene" id="KQJ99029">
        <property type="protein sequence ID" value="KQJ99029"/>
        <property type="gene ID" value="BRADI_3g40620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823180"/>
    <dbReference type="eggNOG" id="ENOG502QQRH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053419_3_0_1"/>
    <dbReference type="OMA" id="FRMEVIS"/>
    <dbReference type="OrthoDB" id="2138282at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.10">
        <property type="entry name" value="PNP-oxidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055343">
        <property type="entry name" value="CREG_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037119">
        <property type="entry name" value="Haem_oxidase_HugZ-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343">
        <property type="entry name" value="CREG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343:SF29">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13883">
        <property type="entry name" value="CREG_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CREG-like beta-barrel" evidence="2">
        <location>
            <begin position="130"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ99029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="366" mass="39607" checksum="69DE7D7AA79F77E7" modified="2012-06-13" version="1">MEALAGAAAASSSLVPSFQARQPTSRVAVLRARPCGPLRAAAAPGGGGGGKDKEEYVATPNGTPVIKLKSDPSQNGALGPIVTDKSRTTLSANTTPDSSGSRAGLFRTPISGGVQSATFAHGLPPPALAVRNLMEQARFAHLCTVMSGMHHRRAGYPFGSLVDFANDSMGHPIFSLSPLAIHTRNLLSDPRCTLVVQVPGWSGLSNARVTIFGDVYPLPAEHQEWAHKQYVAKHQQWASQQWGNFYYYRMQNISDIYFIGGFGTVAWVDVKEYETIQPDKIAVDGGEQSLKELNAIFSKPLREFMSSEGEVDDAALISVDSKGIDIRVRQGAQFNIQRLAFDVPYKVETLEEAKRALHKIIKTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBB7</accession>
    <name>I1IBB7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100829612</name>
        <name evidence="5" type="ORF">BRADI_3g48030v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316675.1">
        <property type="nucleotide sequence ID" value="XM_024460907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBB7"/>
    <dbReference type="STRING" id="15368.I1IBB7"/>
    <dbReference type="EnsemblPlants" id="PNT68990">
        <property type="protein sequence ID" value="PNT68990"/>
        <property type="gene ID" value="BRADI_3g48030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829612"/>
    <dbReference type="Gramene" id="PNT68990">
        <property type="protein sequence ID" value="PNT68990"/>
        <property type="gene ID" value="BRADI_3g48030v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_1_6_1"/>
    <dbReference type="OMA" id="GIVIVPW"/>
    <dbReference type="OrthoDB" id="6159137at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RBD_domain_sf"/>
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    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF17">
        <property type="entry name" value="OS02G0602600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="101"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="188"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT68990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="356" mass="37875" checksum="E8252751DFCBF623" modified="2012-06-13" version="1">MGRKRKRKRRASKDELSEERADPEMAGEGVGEEEGHDDSATNQPPLMLDDCEEGEEEEAIEGLLEPFSRGELLDLLVEACLRDSALLSRLAASAASDATHRRLFVHGLGPGSNSAALAAAFAPFGVLEESHAVADRATGRCRGYGFVTFRRSSSARRALADASKRVGDRPVACQLASLGSAGPCPEMRKLFVDKVPAGASRDELRKFFCQFGEIEAGPLGADHATGRFHRYAIFLYKSPGGLRKALEEPRKVFDGCELHCRPAHSKKRATAEPINADGQINATGSTALPTFHPKEVASTSGMHPLLGSNSPMAKGTSSTEVLFYHNAGAGGAGILGASPLAMAVPSTHSRNTLDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IC51</accession>
    <name>I1IC51_BRADI</name>
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        <recommendedName>
            <fullName evidence="1">ELMO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100840733</name>
        <name evidence="2" type="ORF">BRADI_3g50370v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569999.1">
        <property type="nucleotide sequence ID" value="XM_003569951.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IC51"/>
    <dbReference type="EnsemblPlants" id="KQK00572">
        <property type="protein sequence ID" value="KQK00572"/>
        <property type="gene ID" value="BRADI_3g50370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840733"/>
    <dbReference type="Gramene" id="KQK00572">
        <property type="protein sequence ID" value="KQK00572"/>
        <property type="gene ID" value="BRADI_3g50370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840733"/>
    <dbReference type="eggNOG" id="KOG2998">
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    <dbReference type="OMA" id="ERNGACH"/>
    <dbReference type="OrthoDB" id="67155at2759"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771:SF20">
        <property type="entry name" value="ELMO_CED-12 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="89"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK00572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="269" mass="30652" checksum="D9D0112D256BB6EB" modified="2012-06-13" version="1">MDTNAGSFVAVRRLAGSDRAAGAVAFHHSSSAEVVTGSTAWIGRGLSCVCAQGRDSDARLSFDLTPVQEECLLRLQNRIEVQYDSSNREHQEELKALWCASFPGIELQGLISEQWKEMGWQGKDPSTDFRGGGFISLENLLFFARNYPKSFQELLRKQNGDRAIWEYPFAVAGVNITFMLIQMLDLQAVKPRSLFGAVFLKLLSENDRAFDIIYCVTFKVMDQQWLDMHATYMDFNTVMKATRRQIERELLLEDILRIEDMPSYKLLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1IE15</accession>
    <name>I1IE15_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100839001</name>
        <name evidence="3" type="ORF">BRADI_3g55690v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570417.1">
        <property type="nucleotide sequence ID" value="XM_003570369.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IE15"/>
    <dbReference type="FunCoup" id="I1IE15">
        <property type="interactions" value="1609"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IE15"/>
    <dbReference type="EnsemblPlants" id="KQK01415">
        <property type="protein sequence ID" value="KQK01415"/>
        <property type="gene ID" value="BRADI_3g55690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839001"/>
    <dbReference type="Gramene" id="KQK01415">
        <property type="protein sequence ID" value="KQK01415"/>
        <property type="gene ID" value="BRADI_3g55690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839001"/>
    <dbReference type="eggNOG" id="ENOG502QRTM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053955_0_0_1"/>
    <dbReference type="OMA" id="DVNAFEY"/>
    <dbReference type="OrthoDB" id="64928at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36749">
        <property type="entry name" value="F7O18.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36749:SF1">
        <property type="entry name" value="F7O18.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK01415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="427" mass="46430" checksum="03D01B7604AB91B0" modified="2012-06-13" version="1">MASNDLFEGLPPPATAAAAAAGEDRAASPTSPPPPPAPPVPRPSALKSALKRDKPSSSSDATSSPAVTAAPADAADEGRVPEKRLRFRTTVDASEMQIIEAMQKITSHIGNPSKFSKASKLALQLIEAGSVKPETIGHFFAILEAAMSKPGACNEPSVRADYQALFNAAQGVRECFNQQQKNHFDIWVFHAVVANDLCTDDSFVFSKAVGKIKDAISALPVATMDDDTDEAAALSVAESQSGTMENKADDNKVQSVVSTSLPDDSSTHAAAFNSVEESSDPFGLDDLLEHKSKKSERAREKAVEALNRKADEESKSFLRSQREALLKCLEIAARRYRIPWTQTTIDILGRHAYDNTGRFTRRQRDAVEKLWNSIKEQQIRRKQGKSASGKLDVNAFERLQEKYSHEKISIRRAVGGAGDRRATQWLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-04-02" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMI1</accession>
    <name>I1IMI1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BRADI_4g22050v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the Aux/IAA family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IMI1"/>
    <dbReference type="STRING" id="15368.I1IMI1"/>
    <dbReference type="EnsemblPlants" id="KQJ88915">
        <property type="protein sequence ID" value="KQJ88915"/>
        <property type="gene ID" value="BRADI_4g22050v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88915">
        <property type="protein sequence ID" value="KQJ88915"/>
        <property type="gene ID" value="BRADI_4g22050v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R5WP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117842_0_0_1"/>
    <dbReference type="InParanoid" id="I1IMI1"/>
    <dbReference type="OMA" id="PWELFTI"/>
    <dbReference type="OrthoDB" id="1900465at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF41">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="16"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="176"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ88915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="220" mass="22741" checksum="DE120ED9D8D89BDA" modified="2012-06-13" version="1">MDSSVVIDANPGLSPSRFVKVFMHGKAFGRKINLALHNSYDSLSLTLKRLGSNYSLSQEELNNLAMNEEDGAADDNDFVLWYENVDGDRLFLGAVPWELFTISVKKIYIAPPENQDDNGDYLEEGAGDNGDGNGGAAVGDEVAIGEDEEAPAAAADAAAAGAAEEVGDGVAIAEDEEVLDAAGEAEEAGDGAAIGEVEDAGSVAGDIGDGSVEDADMPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I1IN00</accession>
    <name>I1IN00_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g23920v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IN00"/>
    <dbReference type="EnsemblPlants" id="KQJ89168">
        <property type="protein sequence ID" value="KQJ89168"/>
        <property type="gene ID" value="BRADI_4g23920v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89168">
        <property type="protein sequence ID" value="KQJ89168"/>
        <property type="gene ID" value="BRADI_4g23920v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1847873_0_0_1"/>
    <dbReference type="InParanoid" id="I1IN00"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ89168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="139" mass="14049" checksum="53806AB5E429BDCA" modified="2012-06-13" version="1">MAKETETKEIVALKKIRMDNEREDNSELVFAAHFRGIGLQLKETLHNTELFPSVLRGIGADCNLSLLSRTIAGGSPPTMSTTTGGSPPTTAGYAAVGRHHAGVSVPTTTVGDSLLTAAATVGAGGAGEDDGGGETAGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1IP62</accession>
    <name>I1IP62_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100836080</name>
        <name evidence="1" type="ORF">BRADI_4g27460v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577949.1">
        <property type="nucleotide sequence ID" value="XM_003577901.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238032.1">
        <property type="nucleotide sequence ID" value="XM_010239730.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IP62"/>
    <dbReference type="STRING" id="15368.I1IP62"/>
    <dbReference type="EnsemblPlants" id="KQJ89741">
        <property type="protein sequence ID" value="KQJ89741"/>
        <property type="gene ID" value="BRADI_4g27460v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89742">
        <property type="protein sequence ID" value="KQJ89742"/>
        <property type="gene ID" value="BRADI_4g27460v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89743">
        <property type="protein sequence ID" value="KQJ89743"/>
        <property type="gene ID" value="BRADI_4g27460v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89744">
        <property type="protein sequence ID" value="KQJ89744"/>
        <property type="gene ID" value="BRADI_4g27460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836080"/>
    <dbReference type="Gramene" id="KQJ89741">
        <property type="protein sequence ID" value="KQJ89741"/>
        <property type="gene ID" value="BRADI_4g27460v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89742">
        <property type="protein sequence ID" value="KQJ89742"/>
        <property type="gene ID" value="BRADI_4g27460v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89743">
        <property type="protein sequence ID" value="KQJ89743"/>
        <property type="gene ID" value="BRADI_4g27460v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89744">
        <property type="protein sequence ID" value="KQJ89744"/>
        <property type="gene ID" value="BRADI_4g27460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836080"/>
    <dbReference type="eggNOG" id="ENOG502QPPK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_484328_0_0_1"/>
    <dbReference type="OMA" id="LHMVPDY"/>
    <dbReference type="OrthoDB" id="1911782at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36017">
        <property type="entry name" value="EMBRYO DEFECTIVE 1381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36017:SF1">
        <property type="entry name" value="EMBRYO DEFECTIVE 1381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ89741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="562" mass="62766" checksum="85180B95A1BB96D0" modified="2012-06-13" version="1">MAAAAGGAGGRPWRVIPRPVLETVLHNHALHPRVPQPLLLHGPRGVGKSTLLLHRLLPQWCEPPHSAAFVDFLHPAPSSAAQATAAPWSLLADTVPSLPCLRLRLESAIEGLTRSAVLRGAVGSKDVLAALSRSHGIHSALSRLAGPAARRARGTNPVPVLWARAVLAASSPSHGDDLMFHIDEGEVTNCSMEERAYMQEAMAALRVAKEVLGMQEGWRKEAVREMNRTGRFSRPLANSATDWPCLLLDVLSGAAEEDFFQPKLVLNNVDVLRKATCEDETMVPAAMYHDSFIWRVIALGANEQCLPVIMSTSDGYYSSQAFVDFGFPNIFISRETFGWTPQEAKLHMVSEFFNEQEWKVVDEVLGPNPRLLSEIHMLKQKVNDPGGFHDTNIEEIIDTYLAHLQVSVVNPAMQTALSMVQKFASDVREGKIPENRLCFGAPWRHPPRTDDPDISHKWAKIQLMDFVQSFVKTEFGLNYLADDSLEILDDPAAVAMMEVGLLYQQRDPSFVRPITRGVQRCIARWLVQQRLELSLQESISYLWQRLMRGRSYRHLMKEVGYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQ27</accession>
    <name>I1IQ27_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Legumain prodomain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100823658</name>
        <name evidence="11" type="ORF">BRADI_4g30110v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758778.1">
        <property type="nucleotide sequence ID" value="XM_014903292.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQ27"/>
    <dbReference type="STRING" id="15368.I1IQ27"/>
    <dbReference type="MEROPS" id="C13.002"/>
    <dbReference type="EnsemblPlants" id="KQJ90210">
        <property type="protein sequence ID" value="KQJ90210"/>
        <property type="gene ID" value="BRADI_4g30110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823658"/>
    <dbReference type="Gramene" id="KQJ90210">
        <property type="protein sequence ID" value="KQJ90210"/>
        <property type="gene ID" value="BRADI_4g30110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823658"/>
    <dbReference type="eggNOG" id="KOG1348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024160_0_0_1"/>
    <dbReference type="OMA" id="RWQEEFL"/>
    <dbReference type="OrthoDB" id="192611at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21115">
        <property type="entry name" value="legumain_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.130:FF:000001">
        <property type="entry name" value="Vacuolar-processing enzyme beta-isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000005">
        <property type="entry name" value="Vacuolar-processing enzyme beta-isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043577">
        <property type="entry name" value="AE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048501">
        <property type="entry name" value="Legum_prodom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046427">
        <property type="entry name" value="Legumain_prodom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12000">
        <property type="entry name" value="HEMOGLOBINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12000:SF19">
        <property type="entry name" value="VACUOLAR-PROCESSING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20985">
        <property type="entry name" value="Legum_prodom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500139">
        <property type="entry name" value="AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019663">
        <property type="entry name" value="Legumain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00776">
        <property type="entry name" value="HEMOGLOBNASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095512" description="Legumain prodomain domain-containing protein" evidence="9">
        <location>
            <begin position="22"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Legumain prodomain" evidence="10">
        <location>
            <begin position="365"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR019663-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ90210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="467" mass="50810" checksum="DB2C151DEBA774EF" modified="2012-06-13" version="1">MARLSCFLLLLQAQLFLLVAGEFLRLPSEQDVAGTRWAVLIAGSNDYYNYRHQADVCHAYQIMKKGGLKDENIIVFMYDDIANNPDNPRPGVIINHPTGGDVYAGVPKDYTGKDVNANNFLAALLGDKSKLTGSGSGKVVSSGPNDHIFVYYADHGGPGVLGMPEDESYLYANDLVRALEKKHAGGAGYKSLVFYLEACESGSIFEGLLPGNISVYATTASNAEESSWGTYCPGDVDGAPPAEFDTCLGDLYSVAWMEDSDAHNLKAESLKQQYDRVRDRTSAHETYNLGSHVMQYGDLGINAQSLDIFIGSNPANDKSNSSVSSLLRNARAGVVHQRDADLLHFWHKYKRSAEGSARKHEARRRLVEMMARRARVDGSVELLGGLLFGSEEGAKVMNAVRPAGQALVDDWDCLKDVVRRFEARCGPLTQYGMKHMRALANVCNAGVGVEAVDRAASQACAVHPSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQ36</accession>
    <name>I1IQ36_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100835467</name>
        <name evidence="3" type="ORF">BRADI_4g30170v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578112.1">
        <property type="nucleotide sequence ID" value="XM_003578064.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQ36"/>
    <dbReference type="MEROPS" id="G05.A02"/>
    <dbReference type="EnsemblPlants" id="KQJ90221">
        <property type="protein sequence ID" value="KQJ90221"/>
        <property type="gene ID" value="BRADI_4g30170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835467"/>
    <dbReference type="Gramene" id="KQJ90221">
        <property type="protein sequence ID" value="KQJ90221"/>
        <property type="gene ID" value="BRADI_4g30170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835467"/>
    <dbReference type="eggNOG" id="ENOG502QTD5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="DVPWNWQ"/>
    <dbReference type="OrthoDB" id="548974at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IQ36">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592:SF15">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="241"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ90221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="350" mass="38521" checksum="E333A80C6ED87437" modified="2012-06-13" version="1">MACSSSSSPLSLLLLRPLPRIPLIHSRHRKPFPFPRTGRIAASAAARAQQRLLRPRETGRWRRSLRSGGGFVCLSYSNPPPPSNKDSDEWPVLRRWDVPWEWQTVVLTMVGCGVSFALTGLVEQSVLQYAGYKAAGATIDEKAEILFLGQLSVTAVLLGVIFSITNTFRPFPDDIFRYDIKEPFKLRNGWLLWAGIGLFGAVISIALVGAAMTYLNGEPPEREKDSLVLLLPLIGSSTLSTAYLVGITGVLAPILEETVFRGFLMVSLTKWFPTPVCVVFSAAVFAFAHLTPDQFPQLFVLGVALGFTYAQTRNLLAPITIHAFWNSGVILLLTFLQLQGYDIKELLGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQ84</accession>
    <name>I1IQ84_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">monogalactosyldiacylglycerol synthase</fullName>
            <ecNumber evidence="2">2.4.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100844719</name>
        <name evidence="12" type="ORF">BRADI_4g30600v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Plastid</location>
            <location evidence="9">Chloroplast membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.46" evidence="2"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238179.1">
        <property type="nucleotide sequence ID" value="XM_010239877.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQ84"/>
    <dbReference type="FunCoup" id="I1IQ84">
        <property type="interactions" value="397"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IQ84"/>
    <dbReference type="EnsemblPlants" id="KQJ90291">
        <property type="protein sequence ID" value="KQJ90291"/>
        <property type="gene ID" value="BRADI_4g30600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844719"/>
    <dbReference type="Gramene" id="KQJ90291">
        <property type="protein sequence ID" value="KQJ90291"/>
        <property type="gene ID" value="BRADI_4g30600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844719"/>
    <dbReference type="eggNOG" id="ENOG502QPXV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028367_3_1_1"/>
    <dbReference type="OMA" id="IHPYWIN"/>
    <dbReference type="OrthoDB" id="200404at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046509">
        <property type="term" value="F:1,2-diacylglycerol 3-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17507">
        <property type="entry name" value="GT28_Beta-DGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000111">
        <property type="entry name" value="Monogalactosyldiacylglycerol synthase 3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009695">
        <property type="entry name" value="Diacylglyc_glucosyltr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050519">
        <property type="entry name" value="Glycosyltransf_28_UgtP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43025">
        <property type="entry name" value="MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43025:SF3">
        <property type="entry name" value="MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06925">
        <property type="entry name" value="MGDG_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Diacylglycerol glucosyltransferase N-terminal" evidence="11">
        <location>
            <begin position="157"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="10">
        <location>
            <begin position="353"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ90291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="535" mass="57026" checksum="910E77AF6CF33F65" modified="2012-06-13" version="1">MPPPTASSLAAAADPALPAAFLSVPSPFLPSSATLPFAAAVAAPSHASFLPRARGGSRALAVSVSVTGPTSTAASRLHHMWGEFARFVRLHGNQIAPLGFGSLGLAVGGGGGGGKADGNGGGGGGGGADGVVEEDGMARAEAPKKVLILMSDTGGGHRASAEAIKAAFAQEFGDDYQVFVTDLWTEHTPWPFNQLPRSYSFLVKHGPLWKMTYYGTAPRVIHQPHFAATSTFIAREVAKGLMKYQPDVIISVHPLMQHVPLRVLRSRGLLDKIPFTTVITDLSTCHPTWFHKLVTRCYCPSAEVSKRALKAGLQPSQIKVYGLPVRPSFVKPVQPKDELRRELGMDEDLPAVLLMGGGEGMGPIEATARALDNALYDESLGEPMGQILVICGRNKKLASRLQSISWKVPVQVKGFVTKMEECMGACDCIITKAGPGTIAEAMIRGLPIILNGYIAGQEAGNVPYVVENGCGRFSKSPEHIAKIVADWFGPRSDELQVMSQNALKLARPDAVFKIVHDLHELVRQKSFVPQYACAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQB0</accession>
    <name>I1IQB0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100836491</name>
        <name evidence="20" type="ORF">BRADI_4g30850v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Glutamate-gated receptor that probably acts as a non-selective cation channel. May be involved in light-signal transduction and calcium homeostasis via the regulation of calcium influx into cells.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">May form heteromers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576552.1">
        <property type="nucleotide sequence ID" value="XM_003576504.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQB0"/>
    <dbReference type="FunCoup" id="I1IQB0">
        <property type="interactions" value="601"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IQB0"/>
    <dbReference type="EnsemblPlants" id="KQJ90322">
        <property type="protein sequence ID" value="KQJ90322"/>
        <property type="gene ID" value="BRADI_4g30850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836491"/>
    <dbReference type="Gramene" id="KQJ90322">
        <property type="protein sequence ID" value="KQJ90322"/>
        <property type="gene ID" value="BRADI_4g30850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836491"/>
    <dbReference type="eggNOG" id="KOG1052">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007358_0_2_1"/>
    <dbReference type="OMA" id="WTFMELA"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IQB0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19990">
        <property type="entry name" value="PBP1_GABAb_receptor_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000037">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000103">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000195">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000412">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044440">
        <property type="entry name" value="GABAb_receptor_plant_PBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836">
        <property type="entry name" value="OS06G0188250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34836:SF1">
        <property type="entry name" value="OS09G0428600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="595"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="655"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="833"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="19">
        <location>
            <begin position="472"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="931"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="759"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037090-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KQJ90322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="978" mass="109120" checksum="FF92691BDBC19982" modified="2012-06-13" version="1">MGAVSTLLQAEMRAVLSALLRVLRRHDFLRLVLVLLVFGSSGVTAQGGARRQRQVVDVGVILDMKTWLGNTSWACMEMALEDFYANATQARYRTRLKLHLRDTGPDAVDAASAGVDLLKNVHVQAIVGPQTSTQAKFLAELGNKSSVPIISFSADCPSRSGQTPYFIRTAWNDSSQAEAIASLVKKYNWREVVPVFEDDDSNTRFIPDLVDALRQVDARVSYRCKIHPSAKEDDIRRAISGLKYNWTSVFVVRMSHLLAFKFFQLAKDEGMMGQDFVWITAYGLTDIFDVVGSPALDVMQGVLGVKPHVQDTVELQNFTRRWRKKHRLENPGTSLSEPTVSGLYAYDTIWALALAAEETRYNDSNFVSVTNNGSTDFERIGTSKAAEKLRGTLLKTNFQGMSGKFQIQDMQLLSSKYEIINIVGQEKRVAGFWTPEFSISGNSSSMAELGTIIWPGGNKTPPRGWMLPMNKTLKIGVPVKPGFAEFVKFENGIAKGFCIDVFEAVVRELPYNVSCNYSEFGDGKVSKGTYDDLVYRVYLKEYDAVVGDITILANRSKYVDFTLPYTESGVRMLVPVRDQRQKTAWTFLKPLTTDLWLGTGAFFIFTGFVVWFIEHRTNKDFRGPPASQIGSVFYFSFSTLVFAHKERIVNNLSRIVLVVWLFVVLIVQQSYTASLSSILTVEQLQPTVTNLEEVIRKGSYVGYLNDSFLPGLLKRLKIDESKMIAFDSPAEYNEALSTGKVAVIVDEIPYLKVFLSRYCQNYTMIGPTYKFDGFGYAFPRGSPLTPDISRGILKFASDSKMVELQKTLYGDMSCPDKDDSQISSSLTLHSFQGLFIITGASSMLALILHAVITVYNNQHDLSSDNSLTSWRRWLAIISKLFHEGDSPNTPDKDELAMANVDSIIESPLSIPNHVIERLDSGTDTGSLPEVEGTADRDLSVQDAEPTPSAYVHSERGHNGVASLSRSGSSIRRRQRIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IRC1</accession>
    <name>I1IRC1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC104585122</name>
        <name evidence="4" type="ORF">BRADI_4g33930v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239409.2">
        <property type="nucleotide sequence ID" value="XM_010241107.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IRC1"/>
    <dbReference type="FunCoup" id="I1IRC1">
        <property type="interactions" value="1376"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IRC1"/>
    <dbReference type="EnsemblPlants" id="KQJ90790">
        <property type="protein sequence ID" value="KQJ90790"/>
        <property type="gene ID" value="BRADI_4g33930v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT64849">
        <property type="protein sequence ID" value="PNT64849"/>
        <property type="gene ID" value="BRADI_4g33930v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104585122"/>
    <dbReference type="Gramene" id="KQJ90790">
        <property type="protein sequence ID" value="KQJ90790"/>
        <property type="gene ID" value="BRADI_4g33930v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64849">
        <property type="protein sequence ID" value="PNT64849"/>
        <property type="gene ID" value="BRADI_4g33930v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104585122"/>
    <dbReference type="eggNOG" id="ENOG502QW6T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074924_0_0_1"/>
    <dbReference type="OMA" id="CVSMNTR"/>
    <dbReference type="OrthoDB" id="1909414at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47841">
        <property type="entry name" value="DIACYLGLYCEROL KINASE THETA-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47841:SF3">
        <property type="entry name" value="OS09G0492800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DC1" evidence="3">
        <location>
            <begin position="162"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ90790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="274" mass="30013" checksum="33D8F856D798268C" modified="2012-06-13" version="1">MSSRCVSMHSRNLDGEVPSEDDAHVPETSQHEQEGGGGDGDVSEMVHLSHPEHRLARFDFPYLFMCMGCKEYGAGRRFMCQICGFQLHDFCALAPPSLHDHPFHPRHRHLLFFDKPGGLLRFKCDICGKSVKGFCFRCACCGFGMHPCCAAMTRRMELPAAHGHPLLLAQGSDAGSIIAASFVCQVCRRRRSGRVYQCMPCGYCLHAKCAKDMVNGLYVHGVVPPERSNPLVAAAKVTINAIFGVIGGLVEGIGEGIGEAFVENIGRSRGRSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1ISA1</accession>
    <name>I1ISA1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100833530</name>
        <name evidence="10" type="ORF">BRADI_4g36470v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MS5 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578528.1">
        <property type="nucleotide sequence ID" value="XM_003578480.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ISA1"/>
    <dbReference type="FunCoup" id="I1ISA1">
        <property type="interactions" value="1150"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1ISA1"/>
    <dbReference type="EnsemblPlants" id="KQJ91236">
        <property type="protein sequence ID" value="KQJ91236"/>
        <property type="gene ID" value="BRADI_4g36470v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833530"/>
    <dbReference type="Gramene" id="KQJ91236">
        <property type="protein sequence ID" value="KQJ91236"/>
        <property type="gene ID" value="BRADI_4g36470v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833530"/>
    <dbReference type="eggNOG" id="ENOG502QR54">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013792_1_0_1"/>
    <dbReference type="OMA" id="AERNDNC"/>
    <dbReference type="OrthoDB" id="10258631at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044961">
        <property type="entry name" value="MS5/SDI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36326:SF24">
        <property type="entry name" value="OS09G0538500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36326">
        <property type="entry name" value="PROTEIN POLLENLESS 3-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="188"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="363"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ91236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="508" mass="55987" checksum="AAAE3DD88AE09A3C" modified="2012-06-13" version="1">MMHHQQQQQEQWLAGGVGGLLRPTKSAPCSPVKPAPAPAMLRTNSDAFHVAFKVPVGDTPYVRAKRVQLVDKDPEKAIALFWAAINAGDRVDSALKDMAIVMKQQNRAEEAIEAVKSLRSRCSDQAQESLDNILLDLYKRCGRLDDQISLLKHKLQLIHQGHAFNGKRTKTARSQGRKFQVTLEQEATRLLGNLGWALMQKENYTEAEGAYRRALLIGPDNNKMCNLGICLMKQGRVLEAKDVLKQVRPAGVDGLRGADSHLKAYERAQEMLRDLEIKLVGRPAWATAGDQLDKSWLFDALMLGSSSSIWQPQPCVEHLLPPLPRDQFADENAVGAAASKKMATAAMQPNLNIDAQPFYSHSLRMPPLATKPQNAHRQQPAQQKPVSQLHDPMGNLKRTRSGTAMDKAATAAVEKKEQDDNKNNSGGGGRRKSLSAEERWPELPDHNAFDEALVAAVLAPVLADDENYCSHKPAAVPTSCDTSPAAVKEKVGKRLRIFQDITQTLNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITZ9</accession>
    <name>I1ITZ9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104584772</name>
        <name evidence="3" type="ORF">BRADI_4g41410v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238669.1">
        <property type="nucleotide sequence ID" value="XM_010240367.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITZ9"/>
    <dbReference type="EnsemblPlants" id="KQJ92047">
        <property type="protein sequence ID" value="KQJ92047"/>
        <property type="gene ID" value="BRADI_4g41410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584772"/>
    <dbReference type="Gramene" id="KQJ92047">
        <property type="protein sequence ID" value="KQJ92047"/>
        <property type="gene ID" value="BRADI_4g41410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584772"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001724_0_0_1"/>
    <dbReference type="OMA" id="PWFQTED"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000060">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000120">
        <property type="entry name" value="UDP-glycosyltransferase 76C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF950">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 76C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="51116" checksum="6B7DD4836E5DA1BE" modified="2012-06-13" version="1">MAVVVDDDGLTRGGGRRRIVLFPLPFQGHISPMLQLADVLHRRGHLAVTVLHTARNAPDPAARPDGFDFVPVPDGMPAAVAASDDPLEKILAMNAAMDTSGCVRDALASILLSATEEQQPVSCLIMDTALPAAQKAAAALGLPTVVLHTVSAASTRLYRSYAMLHDKGYLPAQEQELNRPVKELPPLRVSDLFDPSKHPDQESANKILDGNFRTTSNSAGVVINTFEALETPELAALRAELGSTGTSTKVFAIGPLHELSAIDAAASSLQEQDGSCISWLDTQAPGSVLYASLGSVIPVQKDEFAEVAWGLAGSGVPFLWVVRRGLVAGGGESDPDLPDGFERAVEGRGKVVRWAPQREALAHGAVGGFWTHSGWNSTLESVHEGVPMLCRPLFGDQPVNARYVGEAWKNGVLLMEGEKKLERGKIAQAIRAFMDPGEKGDEMRERAEELRRQSAACLEPGGGSTCRAVDELVDHILSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IUS2</accession>
    <name>I1IUS2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100841895</name>
        <name evidence="2" type="ORF">BRADI_4g43670v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578980.1">
        <property type="nucleotide sequence ID" value="XM_003578932.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IUS2"/>
    <dbReference type="FunCoup" id="I1IUS2">
        <property type="interactions" value="2479"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IUS2"/>
    <dbReference type="EnsemblPlants" id="KQJ92433">
        <property type="protein sequence ID" value="KQJ92433"/>
        <property type="gene ID" value="BRADI_4g43670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841895"/>
    <dbReference type="Gramene" id="KQJ92433">
        <property type="protein sequence ID" value="KQJ92433"/>
        <property type="gene ID" value="BRADI_4g43670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841895"/>
    <dbReference type="eggNOG" id="ENOG502QQSV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_417036_0_0_1"/>
    <dbReference type="OMA" id="HCIMQFA"/>
    <dbReference type="OrthoDB" id="1890226at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35118">
        <property type="entry name" value="KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35118:SF3">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ92433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="733" mass="80979" checksum="36306B640EB54CD3" modified="2012-06-13" version="1">MTSTPPARDLDPAAPPTRDLAGLALGAERSRRPRPRHGAHAATPSGQSPDRRGSSSSSSSTCKDFLRKFVDNELLTASLEDWFSGNSQDCGFRKPSFEVPFDLTELQSFDYALEGVTFQQLVRMPNALHASTSDVFEATAHLALEDFLHAGIKGLWEAFWGPEEAMPFSVACIHSTSSRFYPAEKAISSGKLDGVCATAVLLKNSKHSQGRWDHIVVLALLRPDIGMVSAQNDQRPSPAVLGEALFFALRVLLSRSLSRSSTVLRNSDSVYVLLVDSQFGGVVNVQGDLNKLNFDSNNVYECAAEWIKKEAKITVSSVDRVWNKLGNANWGDVGTLQVLLAVFHSMIQFCGAPKYSLDELATEHSSRLQCRRSERHLVDRQSNNGNGLFRFQQRSHSPEIVEVQEEGTVDIKPEEILKLEIGSIVLMEDSSCQKSFQINDIRTESEPPIYGAIPVEEPTRSYLLYVGSSPSHLEPAWEDMNSWYQVQRQTKVLTLMKQRGISSRYIPQMEASGRVVHPGPCNKPNSSGSCGHPLCSTPILVTSPVGETISNLIRNGLFGVEDALRCCHDCLSALAAASSAGIRHGDIRPENVIRVSNGSRPPYFVLIGWGHAILEDRDRPLMNLFFSSTFALQEGKLCAASDAESLIYLLYFSCGGVCPELDSVEGALQWREASWSRRVIQQKLGDVAAVLKAFADYVDSLCGTPYPMDYEIWLRRFRRTINEDHGKEVDTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IUZ7</accession>
    <name>I1IUZ7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NPH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100826918</name>
        <name evidence="7" type="ORF">BRADI_4g44320v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579017.1">
        <property type="nucleotide sequence ID" value="XM_003578969.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IUZ7"/>
    <dbReference type="FunCoup" id="I1IUZ7">
        <property type="interactions" value="682"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92541">
        <property type="protein sequence ID" value="KQJ92541"/>
        <property type="gene ID" value="BRADI_4g44320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826918"/>
    <dbReference type="Gramene" id="KQJ92541">
        <property type="protein sequence ID" value="KQJ92541"/>
        <property type="gene ID" value="BRADI_4g44320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826918"/>
    <dbReference type="eggNOG" id="ENOG502QRW3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005994_6_0_1"/>
    <dbReference type="OMA" id="VCMKQGM"/>
    <dbReference type="OrthoDB" id="624345at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="40"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="NPH3" evidence="6">
        <location>
            <begin position="209"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="601"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="518"/>
            <end position="533"/>
        </location>
    </feature>
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        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="635"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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            <dbReference type="EMBL" id="KQJ92541.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ92541"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="647" mass="71001" checksum="738C92E2CB52AE1A" modified="2012-06-13" version="1">MGLAGDSGAAAFPFSAKLSAANSPRFCNPISRRIFSDVAGDITISVDGQTFLLHKFPLVSRCGRIRRMVADTKDPDLSKLELVNVPGGALAFELAAKFCYGSNFEITTGNVAHLRCIGEYLEMGEDYQGENLIVRTEMYMNDLVVKSLEKSLEVLCGCEGLDPTVEEVGFVDRCVDAIAMNASKEQLVSGLAHLECDAGSGKLRMHCQDWWVEDLSALRIDYYQRVIAAMRRSGVRPESIGTSIVHYAQTALKGIERRHVWDSGPLLGDNQRMIVEALINLMATEKITAVTLSFLFGMLRMAIEVDAGLDYRIEVEKRIGLHLEMASLDDLLIPSLQTSDSMFDVDTVHRILVNFLQRIEEDDSGDSSPCGYESDGLKSPSHSSVLKVGRLMDGYLAEIAPDPYLKLQKFMTLIELLPDYARIVDDGLYRAIDIYLKAHPSLTESECKKLCKLIDCQKLSQDASSHAAQNDRLPMQMAVRVLYFEQLRLKSSFSGGGHFSGGGGGDFSSFSHRIAVPPSGSGVPSSCVSPRGGDSYASLRRENRELKLEVSRMRVRLTELEREQGLMKQGMRGGGGEYGGRPGEHGRALLASISRGIGRMAMFGGPGSTADRRRKGSRTAAASSQSQSQWSDGKKSRRRKKASVTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I1IWC6</accession>
    <name>I1IWC6_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC112269356</name>
        <name evidence="2" type="ORF">BRADI_5g03670v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ81895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="121" mass="13934" checksum="BE383D20DC5BF5ED" modified="2012-06-13" version="1">MSRLQMPAGACFLVDHRPDMFFYKCMNHNPKVKGGCDFWHWEKSYCKYLVKMGYMESNEVDQLRSEKSPEMRTVGREEMKFAVREASVSVGLGKEMLSMLKLAVLLLAGIFVLCLVIVIKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZ84</accession>
    <name>I1IZ84_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC100842706</name>
        <name evidence="1" type="ORF">BRADI_5g14480v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003580006.4"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQJ83356">
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        <property type="gene ID" value="BRADI_5g14480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842706"/>
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        <property type="gene ID" value="BRADI_5g14480v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S1US">
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36743">
        <property type="entry name" value="OS04G0495300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36743:SF1">
        <property type="entry name" value="OS04G0495300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ83356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="162" mass="16391" checksum="F057E4968529A1F6" modified="2012-06-13" version="1">MGLSPSKRVDAALRRAPAFAAACDAAFDRCLADAQRAFEGVRPYQLADASAHLHSALRASLPLVRRWVPSPPPRARVDGALRAAGLDGAGELSREQFGEFAAELFREAVLAGAMGAALVRAPAGAAGIVGVGVVSRAGAGAVGRLVAVYAAGVTAAVYLSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0P1</accession>
    <name>I1J0P1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100827236</name>
        <name evidence="7" type="ORF">BRADI_5g18590v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240272.1">
        <property type="nucleotide sequence ID" value="XM_010241970.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0P1"/>
    <dbReference type="FunCoup" id="I1J0P1">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ84085">
        <property type="protein sequence ID" value="KQJ84085"/>
        <property type="gene ID" value="BRADI_5g18590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827236"/>
    <dbReference type="Gramene" id="KQJ84085">
        <property type="protein sequence ID" value="KQJ84085"/>
        <property type="gene ID" value="BRADI_5g18590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827236"/>
    <dbReference type="eggNOG" id="ENOG502RM8S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062113_0_0_1"/>
    <dbReference type="OMA" id="CLGGEHK"/>
    <dbReference type="OrthoDB" id="551907at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:floral meristem determinacy"/>
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        <source>
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</entry>
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    <reference evidence="8" key="2">
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                <person name="Tice H."/>
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                <person name="Bruce D."/>
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                <person name="Shu S."/>
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            <strain evidence="8">Bd21</strain>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="28"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="TF-B3" evidence="7">
        <location>
            <begin position="266"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ86038.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="381" mass="43708" checksum="A92AF1363884D967" modified="2018-03-28" version="2">MDEEGKGRCERCKEWQEHFYWEHMDVSKMRFFKLMTGDFEQHISIPEKVASKFIRQMQIVEGFDLKAPSGETWHVGVTKVANELFFRLGWRDFAKAHELQENDLVLFTFTGNSSFEVLIFDASGCEKLSSLFSGKMREHFDDLGGQHVEQHSLIDDCDDNDDNDSDDNDDNDSGEDDSESLPSLSVESSHKKSSTSKKFSAKTKPRKEPSQSPTSSSCDDVKHETSDGDESDHEPYYSRSAKRLLDTEKREIIGLALIQPDNPAFMTVLQTSNVQGKHKFLIIPIEFAADHLQRKSHDVLLIRPGREDKWYVRHYQGSSSRGFKCQPWAKFVRDNRLHEGDVCVFELIKCARRKTKKAAMAMMVVHVARRRKADSRFVAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EN90</accession>
    <name>A0A0Q3EN90_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dehydrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g22280v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant dehydrin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EN90"/>
    <dbReference type="STRING" id="15368.A0A0Q3EN90"/>
    <dbReference type="EnsemblPlants" id="KQJ88958">
        <property type="protein sequence ID" value="KQJ88958"/>
        <property type="gene ID" value="BRADI_4g22280v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88958">
        <property type="protein sequence ID" value="KQJ88958"/>
        <property type="gene ID" value="BRADI_4g22280v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828623"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009631">
        <property type="term" value="P:cold acclimation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000167">
        <property type="entry name" value="Dehydrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030513">
        <property type="entry name" value="Dehydrin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33346:SF54">
        <property type="entry name" value="DEHYDRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33346">
        <property type="entry name" value="DEHYDRIN XERO 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00257">
        <property type="entry name" value="Dehydrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00315">
        <property type="entry name" value="DEHYDRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00823">
        <property type="entry name" value="DEHYDRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ88958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="10993" checksum="C91D2D05AAE10FF1" modified="2016-01-20" version="1">MPSKIKDDHKTDGVLRRSGGSSSSSSEDDGVGGRRKKGMKEKIKEKLPGGNKQADEQQMAGVGQQAGHTTGATGTGTGTAGTDGAEATTGEKKGVMEKIKEKLPGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ERL3</accession>
    <name>A0A0Q3ERL3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100846458</name>
        <name evidence="12" type="ORF">BRADI_4g29390v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238135.1">
        <property type="nucleotide sequence ID" value="XM_010239833.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318984.1">
        <property type="nucleotide sequence ID" value="XM_024463216.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318985.1">
        <property type="nucleotide sequence ID" value="XM_024463217.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318986.1">
        <property type="nucleotide sequence ID" value="XM_024463218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ERL3"/>
    <dbReference type="STRING" id="15368.A0A0Q3ERL3"/>
    <dbReference type="EnsemblPlants" id="KQJ90093">
        <property type="protein sequence ID" value="KQJ90093"/>
        <property type="gene ID" value="BRADI_4g29390v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ90094">
        <property type="protein sequence ID" value="KQJ90094"/>
        <property type="gene ID" value="BRADI_4g29390v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ90095">
        <property type="protein sequence ID" value="KQJ90095"/>
        <property type="gene ID" value="BRADI_4g29390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846458"/>
    <dbReference type="Gramene" id="KQJ90093">
        <property type="protein sequence ID" value="KQJ90093"/>
        <property type="gene ID" value="BRADI_4g29390v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ90094">
        <property type="protein sequence ID" value="KQJ90094"/>
        <property type="gene ID" value="BRADI_4g29390v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ90095">
        <property type="protein sequence ID" value="KQJ90095"/>
        <property type="gene ID" value="BRADI_4g29390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846458"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3ERL3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="10">
        <location>
            <begin position="93"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="11">
        <location>
            <begin position="370"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="13"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="31"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ90095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="740" mass="81092" checksum="4BEFBE632823561F" modified="2016-01-20" version="1">MWKWKKKLGHSLSRFLSPKPPFNLAKTKPNLAPPPPPPPPQPPLPSNYPIPPQPAMPHGRPPPPAFPGGHVFPQAASTVLPDPSRFFAPGLLSAPLPTNCFFQNFTLKNGDQPEYIHPYSVKSAAAGLTVCYPTRNHSPTFDIQTFAADLTVSSPSAAAGQLHRIVAFDDLSVTLDFSPSLRAFLVRGCPFVTVATADAAGPVDISVASVHAFIEAVPCDDARTKWRLRMNSGQTFLLYASAPIQLSQSSVTQLAAPGFSGVIRIAYLPDAAMEAVLDQYSRCFPTAGEAALNRPFCVDYTWRKQGWGDLLMLAHPLHLRLLSEDCSARVLDDFRYRSIDGDLVGVVGDSWILKTDPLSPTWHSTRGVNDNGVGEIVAALRKDVDSLASNPITTTSSYFYGKAIARAARLALIAEEVGCPDAIPAVHRFLKATVTPWLDGSFQGNGFLYDPKWGGLITKQGMKDSGADFGFGIYNDHHYHLGYFLYAIAVLAKIDPSWGRKYMSQAYSMVADFMTLSRKCGASYTRLRTFDLWKLHSWAGGLTEFGDGRNQESTSEAVNAYYSAALLGLSYGDTHLVSAGATLTALEMLAAQTWWHVREGEGIYEDDFSGNNRVVGVLWANKRDSGLWFAPPEWKECRLGIQLLPLLPISEALFPDIGFVKDLVSWTAPALARDGVGEGWKGFVYALEGIYDKESALAKTRALTSHDDGNTLTNLLWWLHSRGSVDDGVARCCWYRQYGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FCG4</accession>
    <name>A0A0Q3FCG4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g19589v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FCG4"/>
    <dbReference type="EnsemblPlants" id="KQJ95884">
        <property type="protein sequence ID" value="KQJ95884"/>
        <property type="gene ID" value="BRADI_3g19589v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95884">
        <property type="protein sequence ID" value="KQJ95884"/>
        <property type="gene ID" value="BRADI_3g19589v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FCG4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ95884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="100" mass="11076" checksum="00F388B21A86C832" modified="2016-01-20" version="1">MQCPRVGCVGKVEKGMATIGILGDTRCRYWDCGCGWRVGSKRALLMAEKEDSKDVKIVSPDPPAENHNQKMILVLGSSTRAGDTRLHLQLHHYRINCPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FIL0</accession>
    <name>A0A0Q3FIL0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g43411v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FIL0"/>
    <dbReference type="EnsemblPlants" id="KQJ99479">
        <property type="protein sequence ID" value="KQJ99479"/>
        <property type="gene ID" value="BRADI_3g43411v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99479">
        <property type="protein sequence ID" value="KQJ99479"/>
        <property type="gene ID" value="BRADI_3g43411v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FIL0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ99479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="251" mass="27029" checksum="8B09216C7AE715FE" modified="2016-01-20" version="1">MPRRRRPGPQHQAPAKHKGEFTKDDASKEDHGSQDIAQEAAPRASVIDHQTPLRGTHTAAPLSTMNVAAPIRGQVRASRTHPRPPLTSRGRRLGLLAVDVAPSRHPCQPTPSARLTRSHRAASPLVALLERATPRPQAPRSLTGCHPQIWAAPTSPNARSRQGGRQLQRPEPPLRLPTLEIQPPPRSTPTSTAHQERNPRTTFGSGVRRSGGKAGDDGGKGRSGGEEVGRRPDSAPSVAWVRHGRLEGGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FJ46</accession>
    <name>A0A0Q3FJ46_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106866470</name>
        <name evidence="2" type="ORF">BRADI_3g34945v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756214.1">
        <property type="nucleotide sequence ID" value="XM_014900728.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FJ46"/>
    <dbReference type="EnsemblPlants" id="KQJ98108">
        <property type="protein sequence ID" value="KQJ98108"/>
        <property type="gene ID" value="BRADI_3g34945v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106866470"/>
    <dbReference type="Gramene" id="KQJ98108">
        <property type="protein sequence ID" value="KQJ98108"/>
        <property type="gene ID" value="BRADI_3g34945v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:106866470"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ98108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="124" mass="13609" checksum="C94385A3B2CA1DAD" modified="2016-01-20" version="1">MALMTFLGKFRQMPAMCHERLADGLGAVHEAERDRNAKKKTSAMGTAARTARPAPPPLSYIPRVPLQDSRAVGIIMAVYILRRDFNQQRGGWAEINGSGEQIGERILSSARPPPPPLPPPTERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GE91</accession>
    <name>A0A0Q3GE91_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100836644</name>
        <name evidence="13" type="ORF">BRADI_2g48830v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GE91"/>
    <dbReference type="STRING" id="15368.A0A0Q3GE91"/>
    <dbReference type="EnsemblPlants" id="KQK09576">
        <property type="protein sequence ID" value="KQK09576"/>
        <property type="gene ID" value="BRADI_2g48830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09576">
        <property type="protein sequence ID" value="KQK09576"/>
        <property type="gene ID" value="BRADI_2g48830v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GE91">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12122">
        <property type="entry name" value="AMPKA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14335">
        <property type="entry name" value="UBA_SnRK1_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000204">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000236">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="14"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="12">
        <location>
            <begin position="287"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK09576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="504" mass="57673" checksum="61999C4CCA44E9C0" modified="2016-01-20" version="1">MEGGGKDGNPLKNYRIGKTLGIGSFGKVKIAEHIKTGHKVAIKILNRRKIKSMEMEEKVKREIKILRLFMHPHIIRLYEVIDAPADIYVVMEYVKSGELFDYIVEKGRLQEEEARRFFQQIISGVQYCHRNMVVHRDLKPENLLLDNNCDVKIADFGLSNVMRDGHFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYALLCGTLPFDDENIPNLFKKIKGGIYTLPSHLSGSARDLIPRMLVVDPMKRITIREIREHPWFVAQLPRYLAVPPPDTAQQVKKIDEETLGKVISLGFDKNLLVESIHNRLQNEATVAYYLFLDNKHRTTSGYLGAEYQEAMDSSFSPITPSETQSSAHGNRQQLYMESPVGLRPHFPAERKWALGLQSRANPREVMTEVLKALQELNVYWKKIGHYNMKCRWSPGFPSQENMNHANYNFSVEPIGTDDLGDKLNLIKFEIQVICNYISMLPIRCLDRNGHVMHEYFRVSLHIIVKQCRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H7N4</accession>
    <name>A0A0Q3H7N4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g46083v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H7N4"/>
    <dbReference type="EnsemblPlants" id="KQK19040">
        <property type="protein sequence ID" value="KQK19040"/>
        <property type="gene ID" value="BRADI_1g46083v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19040">
        <property type="protein sequence ID" value="KQK19040"/>
        <property type="gene ID" value="BRADI_1g46083v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H7N4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK19040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="106" mass="12330" checksum="BB3975AAA1E4F236" modified="2016-01-20" version="1">MKGLKRRDCCPNKQKPISTQESKEHVFPAAQKPRVALKEAKPIKNNKKQRRRLRSRSQQRADLGEKLTSDILFWEVRNLHARGPFKPRLRYGLNSLDDSRHRSGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HWJ0</accession>
    <name>A0A0Q3HWJ0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">phosphatidylglycerophosphatase</fullName>
            <ecNumber evidence="10">3.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100831141</name>
        <name evidence="16" type="ORF">BRADI_3g33431v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Exhibits phosphatidylglycerophosphate phosphatase activity. Involved in root growth and columella cells organization. May possess protein phosphatase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:33751"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="EC" id="3.1.3.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33752"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.27" evidence="10"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756601.1">
        <property type="nucleotide sequence ID" value="XM_014901115.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HWJ0"/>
    <dbReference type="STRING" id="15368.A0A0Q3HWJ0"/>
    <dbReference type="EnsemblPlants" id="KQJ97810">
        <property type="protein sequence ID" value="KQJ97810"/>
        <property type="gene ID" value="BRADI_3g33431v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831141"/>
    <dbReference type="Gramene" id="KQJ97810">
        <property type="protein sequence ID" value="KQJ97810"/>
        <property type="gene ID" value="BRADI_3g33431v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831141"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HWJ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14524">
        <property type="entry name" value="PTPMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000051">
        <property type="entry name" value="Dual specificity phosphatase domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044596">
        <property type="entry name" value="PTPMT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46274:SF9">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROPHOSPHATE PHOSPHATASE PTPMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46274">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="123"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="191"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050944"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00053902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQJ97810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="395" mass="43087" checksum="8E2865677B673A57" modified="2016-01-20" version="1">MRIEELPGDSGGGGGGGMVGGGELQPQPQLRCEDGGANGGKLRLRGEDRGVDGGELKLRAEDGGAAVEEGQEESRVEESTGGAVMRAAFDAKRAAVGVGARMLFYPTLVYNVVRNQFESHFHWWDQVDEHVLLGAVPFPSDVLRLQKLGVCGVVTLNESYERLVPKSLYEAHGIENLVLPTRDYLYAPSFDNLCKAADFIHRNASCGKLTYVHCKAGRGRSTTVVLCYLVQYKQMTPAGAFEHVRSCRPRVLLASAQWKAVQEFYQLRVKKTGPSCLDIPIIKPTSSPVFLATQNLITFDDKTFVMVSESDLEGYNADTLSVNVGSSLWEISLVYRVQFASQAAFAGFSYLWLQCRARKDKEALAESVGSESCSLEAEQPANGHPCLLQSVVVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ID66</accession>
    <name>A0A0Q3ID66_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100839831</name>
        <name evidence="11" type="ORF">BRADI_3g36788v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98371.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235159.2">
        <property type="nucleotide sequence ID" value="XM_010236857.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ID66"/>
    <dbReference type="STRING" id="15368.A0A0Q3ID66"/>
    <dbReference type="EnsemblPlants" id="KQJ98371">
        <property type="protein sequence ID" value="KQJ98371"/>
        <property type="gene ID" value="BRADI_3g36788v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839831"/>
    <dbReference type="Gramene" id="KQJ98371">
        <property type="protein sequence ID" value="KQJ98371"/>
        <property type="gene ID" value="BRADI_3g36788v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839831"/>
    <dbReference type="OrthoDB" id="422362at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3ID66">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046975">
        <property type="term" value="F:histone H3K36 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025787">
        <property type="entry name" value="Hist-Lys_N-MeTrfase_SET2_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050777">
        <property type="entry name" value="SET2_Histone-Lys_MeTrsfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22884">
        <property type="entry name" value="SET DOMAIN PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51578">
        <property type="entry name" value="SAM_MT43_SET2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AWS" evidence="10">
        <location>
            <begin position="146"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="8">
        <location>
            <begin position="191"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="9">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ98371.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="37515" checksum="764B268EE55C0A79" modified="2018-03-28" version="2">MPDERMQCSSNRCEATLHKDCSEQYDGSCPRHVCFYCKRGASFHRLKHLMPACTQCFLQTNRMAEPVDPSKTMISCSIWPSTSEGAGLAHGIEEAFRQLPLPYTDREFNIDPIKTEELESLTKPPTYVLLKRNVYIVKHKCDGDAIEGGCTDCDPPLACKTMCSCRSVWISCSRACKCSNECTNRPFRREKRIEVVKTQHCGWGVVALESIQKGDFVIEFVGEVIDDVTCEERLEDMKRRGDQNFYMCKVNKNFVIDATFRGNACRFLNHSCEPNCQLEKWQVNGKTRLGVFASQAIEVGKPLTYSYRFKQHFGPRMECLCGAANCQGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IG41</accession>
    <name>A0A0Q3IG41_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g44496v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IG41"/>
    <dbReference type="EnsemblPlants" id="KQJ99649">
        <property type="protein sequence ID" value="KQJ99649"/>
        <property type="gene ID" value="BRADI_3g44496v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99650">
        <property type="protein sequence ID" value="KQJ99650"/>
        <property type="gene ID" value="BRADI_3g44496v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ99649"/>
        <property type="gene ID" value="BRADI_3g44496v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99650">
        <property type="protein sequence ID" value="KQJ99650"/>
        <property type="gene ID" value="BRADI_3g44496v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IG41"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ99650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="74" mass="8459" checksum="38C4603BECB6CA27" modified="2016-01-20" version="1">MDAKCGAGMEADDLNFYRIGYVQMMRAYRIEFLEGPDKMGCILPGTSSRSAELELCQSLQYLHQTNLKLNYSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IN74</accession>
    <name>A0A0Q3IN74_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhamnogalacturonan endolyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100846621</name>
        <name evidence="4" type="ORF">BRADI_3g57440v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK01668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010237748.3"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3IN74"/>
    <dbReference type="EnsemblPlants" id="KQK01668">
        <property type="protein sequence ID" value="KQK01668"/>
        <property type="gene ID" value="BRADI_3g57440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846621"/>
    <dbReference type="Gramene" id="KQK01668">
        <property type="protein sequence ID" value="KQK01668"/>
        <property type="gene ID" value="BRADI_3g57440v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846621"/>
    <dbReference type="OrthoDB" id="2130367at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IN74">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="RGL4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RGL4_M"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RGL4_N"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Galactose-binding domain-like"/>
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        <property type="entry name" value="RG-lyase_II"/>
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        <property type="entry name" value="Rhamnogal_lyase"/>
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        <property type="entry name" value="OS11G0134100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32018">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="3">
        <location>
            <begin position="427"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="2">
        <location>
            <begin position="514"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK01668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="740" mass="81548" checksum="7B2C62DD6BEAC223" modified="2016-01-20" version="1">MVAGWRRGGSGAGADSGGHYLTVLITRSGAPAATVIMSTAAVLMILQLFAAAAGAPGGGVLLHVESHQVVLDNGVVQVWVSKPQGQITAVRYGDDPGNLLHFTGRSATGVMNSGGYWDLIWNFPGPGRPEGMTDVLDGTEFEVVSWSEEQVEVSFRSTYDPSRQDSVRLNVDKRLVMLRGSSGFYCYAILEHASEWPALNVSQVRLAFKLNPTRFNYMAVSDGIQRYMPAAADRDAPRGTPLAYQEAVLLVNASEPRFTGEVDDKYQYSLDNKDNHVHGWIAAAGDATTVPMGFWIITPSNEFKSGGPTKRELTSHIGPTSVTMFLGTHYVGDDIVAKINAGEQWKKVLGPMFIYLNSSPNPNPKRGDFQFHDMWKDAKAQAKAEVSKWPYSFPVSPDFHKAVERGSVTGRLLVRDRYVSGNDMPARLAYVGLAAPGQPGSWATESKGYQFWTRVWASNGSFAIGDVRAGEYNLYGWVPGFLGDYMYGTTITVASCNNVSLGDLVFEPPRSGRTLWEIGVPDRSAAEFFIPVPNPRNVNKLFLTKDRYRQYGLWERYADLYPAGDPIFTVGESHHSKDWFFAHVTRHVGTDEEASTARHIRFDLASVVTHGIYTLRVALSAAHYSRLELRVNGAPAAAGDRQSAPSMGLFMTPKFGDSNAIARHAEHGTWRSFEFKIEGRLLAVGENNINITQARAFNKFLGVMYDYLRLEAPGSHATSMNIWSWFVILFQCTTGALLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3INS7</accession>
    <name>A0A0Q3INS7_BRADI</name>
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        <recommendedName>
            <fullName evidence="2">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_4g14273v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FHY3/FAR1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQJ87928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3INS7"/>
    <dbReference type="STRING" id="15368.A0A0Q3INS7"/>
    <dbReference type="EnsemblPlants" id="KQJ87928">
        <property type="protein sequence ID" value="KQJ87928"/>
        <property type="gene ID" value="BRADI_4g14273v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87928">
        <property type="protein sequence ID" value="KQJ87928"/>
        <property type="gene ID" value="BRADI_4g14273v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF307">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="529"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="509"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ87928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="551" mass="63839" checksum="4E0D610E76BA4045" modified="2016-01-20" version="1">MLEADPDFKYTVQVDDESRIKNLMWTSGKSMDQYICFGDVLTFDTTYRTNLYDMPFGLFVAVNNHFQSIILGGVLMRDEKVESFKWVFAEFMRMVGGKDRHPKMILTDQWRSQLQKYSRTRNIAKESLGALYGKKSEFRTEFHKLVNLMCTEEEFECAWSEMVEKYGLEKQPFLTQIYEVRRKWAKPYFRDIFCAKMSSTQRSESASANHVLKTYVPPGCPMHLFVKQYEKLQFDRESEESYQEKRTTLSGVVLRANLPIEVHASKVYTRTMFEQFGQTLFESGQYLIEELEQRKKFIVRHSRHVFREKWSKTIYRVEVLLFLDYMEIPKSLVLKRWTRDARDILPPHLRCYERGNGRPRAVTYRHSHLMLAAFEVVRMGDANPTAYEKAIEGMVNLKKTLEPLSVSVDGLGLNEREQSKCTNSKEPAEIREMSSGTQPAVTDLGSKMARADCRGEGSMVDEHGDGAVRVEMPTKKRAAGRPSNAREKPPYEQVSKRSRFCSVWRSAGHKSTTCPKRSNIPNVPRRQPKCSNCGVTGHRKNNCSNPKILLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IYV8</accession>
    <name>A0A0Q3IYV8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g36863v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IYV8"/>
    <dbReference type="EnsemblPlants" id="KQJ91288">
        <property type="protein sequence ID" value="KQJ91288"/>
        <property type="gene ID" value="BRADI_4g36863v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ91288">
        <property type="protein sequence ID" value="KQJ91288"/>
        <property type="gene ID" value="BRADI_4g36863v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IYV8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033238719" evidence="1">
        <location>
            <begin position="30"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ91288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="67" mass="6594" checksum="8F067CE5A2F85D40" modified="2016-01-20" version="1" fragment="single">MVLQSKTAAAIALLLVAVVVAVAVPAASAFGCFDDCYDRCANGLKQDPACTKMCNEACGGGGGVAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JCX4</accession>
    <name>A0A0Q3JCX4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g52121v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JCX4"/>
    <dbReference type="EnsemblPlants" id="KQK10124">
        <property type="protein sequence ID" value="KQK10124"/>
        <property type="gene ID" value="BRADI_2g52121v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10124">
        <property type="protein sequence ID" value="KQK10124"/>
        <property type="gene ID" value="BRADI_2g52121v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JCX4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK10124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="137" mass="14540" checksum="CDDB18244D1E42C2" modified="2016-01-20" version="1">MERGVRGRPDAVPPQAPDRAVRQHQPGRVHVRRRRAGGGTGSGEARGEAGRARAAGVHPGVARAVRDRGPAPGVRRGAGQQQLRVLVQGHAPGWPVGGGGEAVEGHERRRPGGLLRAHAPPRPPRARQPRPPRRLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JGS3</accession>
    <name>A0A0Q3JGS3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Uridine 5'-monophosphate synthase</fullName>
            <ecNumber evidence="5">2.4.2.10</ecNumber>
            <ecNumber evidence="6">4.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BRADI_2g60926v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>orotidine 5'-phosphate + H(+) = UMP + CO2</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the OMP decarboxylase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="5"/>
    <dbReference type="EC" id="4.1.1.23" evidence="6"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JGS3"/>
    <dbReference type="STRING" id="15368.A0A0Q3JGS3"/>
    <dbReference type="EnsemblPlants" id="KQK11572">
        <property type="protein sequence ID" value="KQK11572"/>
        <property type="gene ID" value="BRADI_2g60926v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11572">
        <property type="protein sequence ID" value="KQK11572"/>
        <property type="gene ID" value="BRADI_2g60926v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JGS3"/>
    <dbReference type="OrthoDB" id="10263753at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JGS3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006222">
        <property type="term" value="P:UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000092">
        <property type="entry name" value="Uridine monophosphate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000025">
        <property type="entry name" value="Uridine monophosphate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF40">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pribosyltran"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
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    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="19">
        <location>
            <begin position="248"/>
            <end position="444"/>
        </location>
    </feature>
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        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="16">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="16">
        <location>
            <position position="309"/>
        </location>
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    <feature type="active site" description="For OMPdecase activity" evidence="16">
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        </location>
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        <ligand>
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        <location>
            <position position="276"/>
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        <ligand>
            <name>substrate</name>
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        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>substrate</name>
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    <feature type="binding site" evidence="17">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012321"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00015047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KQK11572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="445" mass="47509" checksum="05589210DCBECE58" modified="2016-01-20" version="1">MDAAAMESLILELHAVEAYKFGTFVLKSGLTSPIYLDLRVLVSHPRLLSAVASLLGDLLPTRPYDLLCGVPYTALPFASVLSVARNIPLILRRYLDSGTSTTTAAMGTQGCFRAGQTVLIVEDLVTSGASVLETVAPLRAEGLLVADAVVVVDREQGGRENLAAHGVTLHSLMTLSEVLAVLVRHGKVSAEKAAEVKRFLDANRKVAVPMPPVAVVRAGRTAFAERAKLAENPVGRKLFQVMEAKQSNLCVAADVGTATELLELANKVGPEICMLKTHVDILSDFTPDFGGKLRSIAEKHNFLIFEDRKFADIGNTVTMQYEGGIFHISDWADIVNAHVIPGPGIIEGLKLKGLPKGRGLLLLAEMSSAGNLAHGGYTAEVVKFAEQHSDFVIGFISVNPAAWSATPSNPAFIHATPEVQIVAGGDALGQQYNTPHRDRLEKKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JNF9</accession>
    <name>A0A0Q3JNF9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein POLLEN DEFECTIVE IN GUIDANCE 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100842089</name>
        <name evidence="8" type="ORF">BRADI_1g10710v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JNF9"/>
    <dbReference type="FunCoup" id="A0A0Q3JNF9">
        <property type="interactions" value="725"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3JNF9"/>
    <dbReference type="EnsemblPlants" id="KQK13524">
        <property type="protein sequence ID" value="KQK13524"/>
        <property type="gene ID" value="BRADI_1g10710v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13524">
        <property type="protein sequence ID" value="KQK13524"/>
        <property type="gene ID" value="BRADI_1g10710v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="29023at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JNF9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="548"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="20"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK13524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="592" mass="66652" checksum="97C93BDBBD09BB5D" modified="2016-01-20" version="1">MSLRSSDRKLSFELLATDLDDDDLDGLSPRSLPESSSDGQRRRRRRAKRKQGFQSPPIVEEEPRVDRDAAAAAFRVTDLMSVVEKVCQSSDAERSVASRVTYVGVELRQRSVSGNGRVVAAPAEDATSSCGSSARESAAAAAAVADVTDAACRPEVNGDVKKLQKEESLDWEKFMKENSNILGEVERLDNSPFRYFIGELYSGNSLRSTIAVGNDKKRQRVYNTMFHVPWRCERLIVAGFFVCLDSFLSLLTIMPARIVMTVWRVLKTRQFLRPNAADLSDYGCFVVLALGVASLQMIDISLIYHVIRGQGTIKLYVVYNVLEIFDKLCQSFGEDVLQVLFNSAEGLSTCSTDNATFELMRFLLDEAIAVVLLLVHSFVLLAQAITLSTCIIAHNNALLALLVSNNFAEIKSNVFKRVSKENLHNLVYYDIIERFHITAFLLFVLAQNILEAEGPWFDSFLVNASLVFMCEVLIDAIKHSFLAKFNEIKPVAYSEFLEDLCKQILNEQSDDRQKDLTFIPLAPACVVIRVLTPVYATLLPVGPFIWRTFWILLWSVLTYFMLAIFKILVGLVLRCLATWYVNLRLTRKQHVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JXU3</accession>
    <name>A0A0Q3JXU3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106865449</name>
        <name evidence="17" type="ORF">BRADI_2g06870v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03289.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754295.1">
        <property type="nucleotide sequence ID" value="XM_014898809.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JXU3"/>
    <dbReference type="EnsemblPlants" id="KQK03289">
        <property type="protein sequence ID" value="KQK03289"/>
        <property type="gene ID" value="BRADI_2g06870v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106865449"/>
    <dbReference type="Gramene" id="KQK03289">
        <property type="protein sequence ID" value="KQK03289"/>
        <property type="gene ID" value="BRADI_2g06870v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:106865449"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JXU3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000187">
        <property type="entry name" value="E3 ubiquitin-protein ligase ATL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF17">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="115"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="163"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="218"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK03289.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="231" mass="23446" checksum="AB4246C93EF09692" modified="2018-03-28" version="2">MSSPGMMTNHTGAAADPYGRQQSFSGRVLLAAVVILFALTVVFVALRVLLYMCCAFGGGGSRGRGGGGLAAGIRRSMNSFGRIGSSRRGLDASALSALPVTAYQKSTGAAGDAECAVCLSELADGDKVRELPNCGHVFHVECVDAWLRSRTTCPLCRAGAEPETELKGNGKEEAAQSSSSSSSAAATGPPQPALSGPGGGSFIVTVHGFPDTRRDAPGSTSGAVQLVSSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K2C8</accession>
    <name>A0A0Q3K2C8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_1g77530v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K2C8"/>
    <dbReference type="FunCoup" id="A0A0Q3K2C8">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK24011">
        <property type="protein sequence ID" value="KQK24011"/>
        <property type="gene ID" value="BRADI_1g77530v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK24011">
        <property type="protein sequence ID" value="KQK24011"/>
        <property type="gene ID" value="BRADI_1g77530v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K2C8"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K2C8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF21">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="257"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="292"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="327"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="397"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK24011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="52193" checksum="1C19A55ECFDF4C91" modified="2016-01-20" version="1" fragment="single">MHSPAGCPATTSSRAIRPSFVPMAQRGSRGREQRLPSSSSSAPDAELASILRAEAAVSGVSRKATAAVSQQQQSTRLWPRAVLEALDSAVASCHWESALEIFELMRKQRWYEPRTRTYARLLMMLGKCRQPGPAAALFRVMLSEERLAPTVDVYTALVGAYGYSGMLDEALATVELMKGAADCKPDEYTFSILINCCCKSRRFDLIPPVLDEMSYLGIGCNVVIHNAIIDGYGKAGMLAEMGTALCNMLEDGDNVPDIYTMNSIIWAYGNRGRVDDMEKWYSEFQLMGVEPDSRTFNIMIKSYGKANMHEKMMMVLKYMKRRFFSPTAATFNIIIECFGRAGDIEKMEYYFRLMKIQGIKPNPITYCSLVNGYSKAGILDKVPAIIRQTENTDVVLDTPFFNCVINAYAKSGDIKIMEEMLQLMKEKKCKPGKVIYTTMIQAYIAHGMDEAAKLLEIEVDSFGNRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KE71</accession>
    <name>A0A0Q3KE71_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="9">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100839637</name>
        <name evidence="10" type="ORF">BRADI_1g69160v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="9"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558433.1">
        <property type="nucleotide sequence ID" value="XM_003558385.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KE71"/>
    <dbReference type="FunCoup" id="A0A0Q3KE71">
        <property type="interactions" value="1440"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3KE71"/>
    <dbReference type="EnsemblPlants" id="KQK22748">
        <property type="protein sequence ID" value="KQK22748"/>
        <property type="gene ID" value="BRADI_1g69160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839637"/>
    <dbReference type="Gramene" id="KQK22748">
        <property type="protein sequence ID" value="KQK22748"/>
        <property type="gene ID" value="BRADI_1g69160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839637"/>
    <dbReference type="OrthoDB" id="409992at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KE71">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825:SF29">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK22748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="408" mass="43845" checksum="1583A9890BB98186" modified="2016-01-20" version="1">MAVLSSAKRVLPWASCNGHALARVSGGLRGLLGTADGAAAAGGCSLLPCRWRSSLPQLDSVDRSDEDSSGGDIDWDNLGFGLTPTDYMYVMRCSQGDAGFSRGELSRYGNVELSPSSGVLNYGQGLFEGLKAYRRSSEMGGYTLFRPEENARRMQVGAERMCMPAPSVEQFVHAVKQTVLANRRWVPPQGKGALYIRPLLIGSGAILGLAPAPEYMFLIYAAPVGTYFKEGLAAINLLVEEEIHRAMPGGTGGVKTIANYAPVLKPQMDAKSKGFADVLYLDPVHKRYVEEASSCNLFVVKGGAVATPATAGTILPGITRKSIIELARDRGYQVEERLVSIDDLVSADEVFCTGTAVGVTPVSTVTYQGTRYEFRTGQDTLSRELYTDLTSIQMGLAEDKKGWTVAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KIG9</accession>
    <name>A0A0Q3KIG9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100825408</name>
        <name evidence="9" type="ORF">BRADI_2g57201v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567341.1">
        <property type="nucleotide sequence ID" value="XM_003567293.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KIG9"/>
    <dbReference type="STRING" id="15368.A0A0Q3KIG9"/>
    <dbReference type="EnsemblPlants" id="KQK10945">
        <property type="protein sequence ID" value="KQK10945"/>
        <property type="gene ID" value="BRADI_2g57201v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825408"/>
    <dbReference type="Gramene" id="KQK10945">
        <property type="protein sequence ID" value="KQK10945"/>
        <property type="gene ID" value="BRADI_2g57201v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825408"/>
    <dbReference type="OrthoDB" id="1849108at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051032">
        <property type="entry name" value="AP2/ERF_TF_ERF_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31985:SF45">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31985">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF042-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="86"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK10945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="210" mass="22460" checksum="48E4A00A46C8141A" modified="2016-01-20" version="1">MQVNSSAGLTLPHPIYAAQNVRALGLSKLKLDQQPAQPQKSEIQCEVAELKRLGIKLGRSQKVAMSRVDEAARGAGNGEGGEEECRYKGVRRRRWGKWVSEIRVPGSRERLWLGSYATPQAAAVAHDAAVFFLRGGGQGATDAAALNFPERAAATYGMGTSAAAARLSPRSVQRVASDAGMAADAQLVEAREQQRLASGEISVDDMEILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3MUR3</accession>
    <name>A0A0Q3MUR3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">beta-galactosidase</fullName>
            <ecNumber evidence="4">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BRADI_2g39450v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="4"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3MUR3"/>
    <dbReference type="EnsemblPlants" id="KQK08078">
        <property type="protein sequence ID" value="KQK08078"/>
        <property type="gene ID" value="BRADI_2g39450v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08078">
        <property type="protein sequence ID" value="KQK08078"/>
        <property type="gene ID" value="BRADI_2g39450v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3MUR3"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22842">
        <property type="entry name" value="Gal_Rha_Lectin_BGal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025300">
        <property type="entry name" value="BetaGal_jelly_roll_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041392">
        <property type="entry name" value="GHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13364">
        <property type="entry name" value="BetaGal_ABD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17834">
        <property type="entry name" value="GHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033239096" description="beta-galactosidase" evidence="12">
        <location>
            <begin position="19"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="13">
        <location>
            <begin position="525"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK08078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="608" mass="65690" checksum="C4D115A820E16D53" modified="2016-01-20" version="1">MDRILPLLLLAAAAVVLGAGGTEISHNNRALVIDGERRIIVSGSIHYPRSTPEMWPELIRKAKEGGLDAIETYVFWNGHEPRKRQYNFEGKGLPAWLRDISGMQFRMHNDPFEKKMETFTSLIVAKLKEAKLFAGQGGPIILSQYTLDNSSSTSATSSSTSACFISNKFEDRDVNVTLDDGATHVIVPAWSVSILPGCKAVAYNTARIKTQTSVMAKKPHQLPETTTAAGQQQPMWSWMPENLAPFMTDEKGSFRKNELLEQITTSADESDYLWYRTSFEHKGEASYKLHVNTTGHELYAFVNGKLVGKHHSPNGGFVFQLETPVKLHSGKNYISLLSATIGLKNYGALFEMMPAGIVGGPVKLIKTTSNTTAMDLSNSSWSYKSGLAGEYRSIHLDGDDTRQQWQSLNGTIPVHRPFTWYKATFSAPAGSEPVVADLLGLGKGVAWVNGHNLGRYWPSYVASDMAVRLQRHLQGGRGRGPVPLGVRGARPAVLPRLVLFEEAGGDPSAAAFKTVGVGAACVEGVQVGDDVALACGGHGRTISSVDVASVGVTRGRCGGAYEGGCESEKALEALKKACVGKESCTVRHTEEFLVAAGVSGELTVQVTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R277</accession>
    <name>A0A0Q3R277_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g36316v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R277"/>
    <dbReference type="STRING" id="15368.A0A0Q3R277"/>
    <dbReference type="EnsemblPlants" id="KQK07570">
        <property type="protein sequence ID" value="KQK07570"/>
        <property type="gene ID" value="BRADI_2g36316v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07570">
        <property type="protein sequence ID" value="KQK07570"/>
        <property type="gene ID" value="BRADI_2g36316v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3R277"/>
    <dbReference type="OrthoDB" id="1924069at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3R277">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039342">
        <property type="entry name" value="TGD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34675">
        <property type="entry name" value="PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34675:SF1">
        <property type="entry name" value="PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK07570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="114" mass="12783" checksum="1F021728A817B32A" modified="2016-01-20" version="1">MQEIGVDKGYKLVEKVVHVMEKARPLISKVHDSNLLKDVEIIAKGLADASDDLRWLKSSVLTVENTDLIKKSIFTIIYTLKNIESISSDVSGFTGDEATTRNIKLLIKSLRRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RNM4</accession>
    <name>A0A0Q3RNM4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g17560v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14602.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RNM4"/>
    <dbReference type="FunCoup" id="A0A0Q3RNM4">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3RNM4"/>
    <dbReference type="EnsemblPlants" id="KQK14602">
        <property type="protein sequence ID" value="KQK14602"/>
        <property type="gene ID" value="BRADI_1g17560v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14602">
        <property type="protein sequence ID" value="KQK14602"/>
        <property type="gene ID" value="BRADI_1g17560v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3RNM4"/>
    <dbReference type="OrthoDB" id="693375at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RNM4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046533">
        <property type="entry name" value="DUF6598"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065:SF163">
        <property type="entry name" value="OS05G0112700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065">
        <property type="entry name" value="OS07G0486400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20241">
        <property type="entry name" value="DUF6598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6598" evidence="2">
        <location>
            <begin position="134"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK14602.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="333" mass="37436" checksum="C5620966613CCD44" modified="2018-03-28" version="2">MAEDEDGEVLPAYIEEDEEEASAAKEQRRQQSTKPKKRWPWEFATPEEEAKNKARMDLLDKHYEHDPKTGHGSYVRAWFVDPSDLDVETQYGPMRHTDSIIPDDHRLRDSLNVLCLKTVSSDVGYPISVYGTDKNEDLILAGPTRGLFYTGEAFFEINLKIKEDEECNDRQFTKALIDVLLARSPKVSTRTVPGRLSEVQLVCAYVKNALEGTFEIKIRSGPEVFCGNRTACTTDVPNNIILYDSDVGGGMAVADDGIIQLFRRVVAVSEDEILILNIDACGSDLNVNISRCISMFTPRIKGADAEEVTCGLYKIRVEVTWSMSVMVTFYLVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RZD0</accession>
    <name>A0A0Q3RZD0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter C family member 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Multidrug resistance-associated protein 13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">OsMRP5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Protein LOW PHYTIC ACID 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100841508</name>
        <name evidence="19" type="ORF">BRADI_1g37826v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ABC transporter that may affect phytic acid transport and compartmentalization. May function directly or indirectly in removing phytic acid from the cytosol or in vesicle trafficking. Required for phytic acid accumulation in developing seeds. Phytic acid is the primary storage form of phosphorus in cereal grains and other plant seeds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010227598.1">
        <property type="nucleotide sequence ID" value="XM_010229296.3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK17970">
        <property type="protein sequence ID" value="KQK17970"/>
        <property type="gene ID" value="BRADI_1g37826v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841508"/>
    <dbReference type="Gramene" id="KQK17970">
        <property type="protein sequence ID" value="KQK17970"/>
        <property type="gene ID" value="BRADI_1g37826v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841508"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RZD0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000003">
        <property type="entry name" value="ABC transporter C family member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000169">
        <property type="entry name" value="ABC transporter C family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000002">
        <property type="entry name" value="ABC transporter C family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001405">
        <property type="entry name" value="Multidrug resistance protein associated1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF108">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="295"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="517"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="912"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="942"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1021"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1047"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="18">
        <location>
            <begin position="296"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="610"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="18">
        <location>
            <begin position="921"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="1228"/>
            <end position="1462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQK17970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1473" mass="162550" checksum="00AD76550B8EF362" modified="2016-01-20" version="1">MREAGMAGDNLGYPATALVDGSSSWSSWICEQEGGGITFASPCVQRSLIDCVNVVLLVAYAIALLVTCVRGQWDGRGRRRRRWEVAAVSVCCVAVAATYAVIGFRDATDAAASIVRGVVWVSVAASLQVQPTRPASTVALLWWTLFSLLITAYNAEVLISGYRLDVAEVVAWPVNFLLLLCALSSLLQRSDGRKDSLDDNGLSEPLIDKAVHDSELYRAGLFSQLTFSWLNPLLRLGRSKALDLADVPLIGSEDSALQASKKFSEAWNRHRQDKARSGSTNGLPLVLFKCFLREIVIAGFYALMRTLAIAVSPALLFAFVRYSYQEERDLRVGLSLVGCLLLIKLVESLSQRHWFFDSRRTGMRIRSALMAVIFEKQLKLSSQGRKNHSTGEIVNYIAVDAYRLGDALSWLHMAWSSPLQLALAVGTLIWALRLGAVPGLVPLIIFGFLNVPFAKLLQGYQAKFMVAQDERLRSTSEILNSMKIIKLQSWEEKFRSMIESLRDAEFKWLRETQMKKAYGAVMYWMSPTVVSAVMYTATAILGSAPLNASTLFTVLATLRVMAEPVRFLPEILTMMIQYKVSLDRIEKFLIEDEIKEGVERVPSDNSDIRVHVQDGNFSWNASGADLALRNVNLRIRQGEKVAVCGAVGSGKSSLLYALLREIPRTSGSVEVFGSLAYVSQNSWIQSGTVRDNILFGKPFNKELYEKAIKSCALDKDIENFDHGDLTEIGQRGLNMSGGQKQRIQLARAVYNDADIYLLDDPFSAVDAHTAAVLFYDCVKTALSKKTVVLVTHQVEFLTETDRILVMEGGQVNQQGKYAELLESGTAFEKLVSAHQSSVTALDTTSQQNQVQGQQVLDDSISPSALLATRQSSDIEVQTKGPSMIQLTEEEEKGIGDLGWKPYKDYIDVSKGFLPLCGMCTAQVLFTCFQIMSTYWLAVAVQINVSSALLVGAYSGLSIFSCCFAYLRSLFAATLGLKASKAFFTGLMDSVFKAPMSFFDSTPVGRILARASSDLSILDFDIPYSMAFVATGGIEVVTTILVMSTVTWQVLVVAIPVAITMVYVQRYYVASARELVRINGTTKAPVMNYAAESILGVVTIRAFAATDRFIRNNLQLVDNDATLFFHTVAAQEWVLIRVEALQSLTILTSSLFLILVPPGVISPGFAGLCLSYALTLTSAQVFLTRFYSYLENYIISVERIKQYMHLQSEPPAIIPDNRPPTSWPHEGRIDLQDLKVKYRPNTPLVLKGITCTFPAGNRIGVVGRTGSGKSTLISSLFRLVDPVGGRILIDNLDICSIGLKDLRAKLSIIPQEPTLFRGTVRNNLDPLGLHSDDEIWEALEKCQLKRSISSTAALLDTVVSDDGDNWSVGQRQLFCLGRVLLRRNKILVLDEATASIDSATDAILQSVIRKQFTSCTVITIAHRVPTVTDSDRVMVLSYGKLLEYDTPAKLLEDKQSAFAKLVAEYWANCKRNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S7Y2</accession>
    <name>A0A0Q3S7Y2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g59163v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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</entry>
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                <consortium name="EnsemblPlants"/>
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    </evidence>
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</entry>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">Bd21</strain>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CKY0"/>
    <dbReference type="EnsemblPlants" id="PNT62687">
        <property type="protein sequence ID" value="PNT62687"/>
        <property type="gene ID" value="BRADI_4g07197v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62687">
        <property type="protein sequence ID" value="PNT62687"/>
        <property type="gene ID" value="BRADI_4g07197v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CKY0"/>
    <dbReference type="OrthoDB" id="1060521at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CKY0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080115">
        <property type="term" value="F:myosin XI tail binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007656">
        <property type="entry name" value="GTD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31422">
        <property type="entry name" value="BNAANNG28530D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31422:SF0">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04576">
        <property type="entry name" value="Zein-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51775">
        <property type="entry name" value="GTD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GTD-binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT62687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="408" mass="43851" checksum="BE227C49D0094BD3" modified="2018-03-28" version="1">MTMILRLQREKAEAMMEARQFRRYAEEKMSHDAAELASLEEAIAKRDATAAATAMRGVPSQSRLGGGTPRCGLSIPSSPRVSSTPRHASSTPSSSVAAAAAAAASGGYYPALRCFNDMQQHPPTASEADALDVQTPRDHLARLSHRVNMLERRAVAAPQPSITTPIIRVAPGSAFQQRNPRAFSDADSLDLCDGEFFPDDVDCGASDRVYTVDAIHGRGGGVAMAVPEGSYCGGTPVGSVSDCCGGGAPWGEDEEMRRLSARLRALEADRENMRQAILSMGAEKAQVVLLKEIAQQLCKDGPPTSSLPTVTVGQHYYKGAAPAPAMTVTVPRPQRPMVMHRMELKSQPKTSLFATVVKWVASIVWWRRKSARIKHPIGQCGNNVGLLLLLDKSFKAAGNGRQKPPKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CKZ0</accession>
    <name>A0A2K2CKZ0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucose/Sorbosone dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100830347</name>
        <name evidence="4" type="ORF">BRADI_4g07210v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757756.2">
        <property type="nucleotide sequence ID" value="XM_014902270.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CKZ0"/>
    <dbReference type="EnsemblPlants" id="PNT62690">
        <property type="protein sequence ID" value="PNT62690"/>
        <property type="gene ID" value="BRADI_4g07210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830347"/>
    <dbReference type="Gramene" id="PNT62690">
        <property type="protein sequence ID" value="PNT62690"/>
        <property type="gene ID" value="BRADI_4g07210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830347"/>
    <dbReference type="OrthoDB" id="10266706at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CKZ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328">
        <property type="entry name" value="HEDGEHOG-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328:SF73">
        <property type="entry name" value="PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043158495" description="Glucose/Sorbosone dehydrogenase domain-containing protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="678"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose/Sorbosone dehydrogenase" evidence="3">
        <location>
            <begin position="382"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT62690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="744" mass="80908" checksum="F15E387792A0DFA8" modified="2018-03-28" version="1">MAMGSSARMTIFLSLLLVYSSSLQLPARSLPLCTDMRAPVVLNVTLKFCRNDAGGSSSCCDAAADAALQAQFDAMDVKPADGECARLVKSILCSKCNLFSADLFDIGSMRRTVPLLCSSFSAQDSSQHKHPTHNNGDYCGQVWKYCKSTAMSNSPFRPFAWRKVGLIGSSSMLTDFWQSEKDFCGSLSDTPKNQLVCFNGHGVSFNTRRNSSPAPNGICLEKISNGTYLNMVAHPDGSSKAFFCRQDGKIWLATVPEQGTRDSLQLDETIPFLDLATEGHLSSELGLVGVAFHPDFVNNGRFFVSYICDGTQSSNCAGRCSCDREVGCDPSKLRPDNGVVPCRYQLLISEYSAKGSSSSFSEATYADPSEVRRVFSMGLPYVSNHAGQLLFGPTDGYLYFFTGHGGIRGDPFNFSQNGKSLLGKVLRLDIDELPEMNEVSNKSLWGNYTIPKDNPHTGDSNLQPEIWALGLENPWRCSFDSLRPFHLYCADDGQEQYKVVDLISKGGNYGWSGAYKDQDVHYPPWASQGTKPTNGIIFPIMGYKVPSTTESAAIVGGHVYRGSADPCLYGRYLFADMYSSAMWTGTVNTDGSGKYTSASIPLSCSEKTPLPCDDSTDSPLGPIFSFGQDNKQDGFILVSQGVYRIVQPSLCDYVCVNDATTEQAAASVSVGSQGLPTILKALIGVVVSVFGTAGAIYLVRTCFCNNSVILGNRIATMQVINNTTTAEIILPWRQQPKLVTLNWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQ52</accession>
    <name>A0A2K2CQ52_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">5'-3' exoribonuclease</fullName>
            <ecNumber evidence="6">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_4g25132v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Possesses 5'-&gt;3' exoribonuclease activity. Acts as an endogenous post-transcriptional gene silencing (PTGS) suppressor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="6"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQ52"/>
    <dbReference type="FunCoup" id="A0A2K2CQ52">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CQ52"/>
    <dbReference type="EnsemblPlants" id="PNT64151">
        <property type="protein sequence ID" value="PNT64151"/>
        <property type="gene ID" value="BRADI_4g25132v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64151">
        <property type="protein sequence ID" value="PNT64151"/>
        <property type="gene ID" value="BRADI_4g25132v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQ52"/>
    <dbReference type="OrthoDB" id="372487at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017151">
        <property type="entry name" value="Xrn2/3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF62">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037239">
        <property type="entry name" value="Exonuclease_Xrn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="9">
        <location>
            <begin position="315"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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        <location>
            <begin position="606"/>
            <end position="692"/>
        </location>
    </feature>
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        <location>
            <begin position="647"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="665"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006994"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR037239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF03159"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT64151.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT64151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="692" mass="78544" checksum="9DCADD1B6D16693F" modified="2018-03-28" version="1">MNGIIHPCFHPEDPSSSSPAPATFDDVFRAIFAYTDHLLRIARPRKLLYLALDGVAPRAKMNQQRARRFKSAIAAKDAEVEEKLLRERFRAEGKELLPPPPVPVDAEALLDPNVITPGTEFMEKLSAALQYYVRARLNAHPRWKHLKVIMSDANVPGEGEHKIMSFIRAQWSMESYDPNTSHCLYGHDADLIMLALASHEVHISILRKYLELEMTTPGYKHDTERLIDDFVSICFLMGNDFIPQIPSLEIHEFGVDLLIEMYKTTFNEMGGYILNTDKIKDKHAAYLEVSRLEKFLHALSLCEEKIFLKRYELRQIRLGSPGWKSRFYREKFGAETSNEVVRLQAEMVQKYLEGLCWVLQCYFDDVPSWTCYYPFHYAPFASDLKHLSQFNIYFTMDKALKPFDQLMAILPPQMHLFSCAFPKCYSKLIGFEQSTIQLFYPTELEIDAHRKHFLSDGVEKLPFIDEKLLLSATKTGEKDLTVQETSRNTIRQERIFLRNSNNLANDAAFVAMYDCPEEKHRISTSEIGGCLSPDFDAPTHSFIRSPVNDMRPIVGDQTMQVFLSAAFSNPDVAKPITRLLNNVVVPEKTVTESEIRKRRLWHTYPGARPPPVARKPETLWKASSAAMPRDEIKPAGTGWLGRGRGSAAGPSAAAAAAATETRQIGRSSAAPAWRPARGRGHGGRGNPRPRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQG9</accession>
    <name>A0A2K2CQG9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">X8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_4g26820v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A2K2CQG9"/>
    <dbReference type="EnsemblPlants" id="PNT64272">
        <property type="protein sequence ID" value="PNT64272"/>
        <property type="gene ID" value="BRADI_4g26820v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64272">
        <property type="protein sequence ID" value="PNT64272"/>
        <property type="gene ID" value="BRADI_4g26820v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQG9"/>
    <dbReference type="OrthoDB" id="1073427at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CQG9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="X8"/>
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        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF120">
        <property type="entry name" value="CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
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            <end position="26"/>
        </location>
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            <begin position="27"/>
            <end position="353"/>
        </location>
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            <begin position="330"/>
            <end position="349"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT64272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="353" mass="36066" checksum="3225265EBA561B9F" modified="2018-03-28" version="1">MEVRFLLLLLLPACSLLFLHLHSASGKPTVRDNLDPAQVTNPAMPITVPSTNPTPTIITVPSTNPTVTIPSLNPLPTPITTPSADPSTTLPLPTPSTSAPNTPVTNPVTTPSTVPSSAPLTNPAANPMAPTIGITPPPAAPVTTPVTAPAVSGQQAWCVAKAGSAETALQDALDYACGIGGADCLPIQPSGSCYYPNTLEAHASYAFNSYYQKNPAPSSCNFGGAAMLANANPSSGSCVLASSMSSPTRWKLLTWHSGNMKFLCLWYSTAGYNPGSVSPTTSSTNPVTGASGSDPGSSVLNASGSGISGSSDFGSDFPGEANKGNSWRSILPFGWCWAGLFSILALPYVGGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CRC2</accession>
    <name>A0A2K2CRC2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g30368v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CRC2"/>
    <dbReference type="STRING" id="15368.A0A2K2CRC2"/>
    <dbReference type="EnsemblPlants" id="PNT64579">
        <property type="protein sequence ID" value="PNT64579"/>
        <property type="gene ID" value="BRADI_4g30368v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64579">
        <property type="protein sequence ID" value="PNT64579"/>
        <property type="gene ID" value="BRADI_4g30368v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CRC2"/>
    <dbReference type="OrthoDB" id="1743921at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT64579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="16998" checksum="460FBBA6C978AF38" modified="2018-03-28" version="1">MLVATYEGEHNHGQSPEREFIGNGSTDQAGSRPCSIGINSLCRTTMLDLTNQGSGSSMEGIARGVVTPEFHKLLVEKMVDSLKNDAEFMGALTSAVAEKVVERILDRAAHVLPSPSCAPISAAPTASTDANGRAVSASQGRARCIPATASLPSCDRASSGPAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CSV1</accession>
    <name>A0A2K2CSV1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g37322v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
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                <person name="Shu S."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65104"/>
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    </evidence>
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    </evidence>
    <sequence length="54" mass="6145" checksum="95958B8B11350DA4" modified="2018-03-28" version="1">MHTPKENSVAKCRFANYFVVDMPVPRVKNAEGNTLAAKIHNDDLHIRCIICVYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="9">cv. Bd21</strain>
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    </dbReference>
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</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <strain evidence="1 2">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
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        <source>
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    </evidence>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8 9">Bd21</strain>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF28">
        <property type="entry name" value="OS12G0133000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
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    </dbReference>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
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            <begin position="200"/>
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            <begin position="234"/>
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    </feature>
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            <begin position="333"/>
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    </feature>
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            <begin position="162"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="603" mass="65438" checksum="C3E46B436F8C923F" modified="2018-03-28" version="1">MATSYSQKGAYTRRICDELLTKVMCIPETLGVIRPLVDAVVEGEGAGEAEAVPPVFLAAGVVGGAGEEAKLLYIASKAQPSRCYKAGSISAHSSQRKRYILPYIAFHPSILMKSTHRNWESMGGGGEEGGAPLLAAWSWEGCPGCAMERRKESCAGRIPYTELFFVGLTTLASSLPITCLFPFMYFMVRDFHIAKTEEDIGSYAGFLASSYMVGRACSAIFWGIVADRIGRKPVIAFSILSVVIFNTLFGLCTEYWMAIATRLVLGSLNGLLAPIKAYAIEVCQAEHQALGLSVVNTAWGFGLVIGPALGGYLAQPAEKYPQTFSKDSVFGRFPYLLPSLGVSLFAAIVLISCIWLPETIHKHGIREKGIQIVKSLASEEAYWDSPRKKSLLQNWPWMSTMVPYCLFGLHDTAYSEILSLWAVSDRKYGGLSFSSEDIGEVLAVAGASLLVYQLFIYRWVHKFLGPVISARVGSALSILIVSTYPFMTYLSGAKLSLALYSAAMMRGIVAITASTGVCLLQNSAVRQEQRGTANGVSTTAMSFFKAVAPVGAGILFSWAQKHQDSAFFPGDQVVFLALNLVQILGLIFTFEPFLVLPVVEECS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D258</accession>
    <name>A0A2K2D258_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BRADI_3g39273v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodium</taxon>
        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
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    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT68343">
        <property type="protein sequence ID" value="PNT68343"/>
        <property type="gene ID" value="BRADI_3g39273v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g39273v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
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            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
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            <dbReference type="EMBL" id="PNT68343.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT68343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="474" mass="49468" checksum="02414539185E6F5E" modified="2018-03-28" version="1">MEACPPSSEPETSVGAASSARGATGAVGAAGAGEDEAGATTAGTASDGRGAVTGGAGAGRGTGAVVIAAEAGAAGVGAGAVRAGRGAPLKRKVSARVPANPATTTSSPASRVQPWRRPLRLERGSNHDGCRAVAALPGAADPSSVPSVRLRAQLKRRRRQHLLPPPDSPLRRASSSHGRRRPSSSLSTSAPPSPHGPLSRAPSLPWLPAAHPAPLSLCAGAASTSHALARASRSPRLATPRRRRRPGRAAHCRAPPIADPHDHALRRPRPPFPCSPLLRPPPCAPLAELASTSQRRSASPSPSATSLPCSPRCRFSASAARCSSLPLSRPPARRPSSSSLAHLQAQVQQARAFSSLWSFLNFFSGNCFFLMCGPCWSAPPCAPRVFQRAAAQVLALRTKRPKDFRAGAMNDYVDYIACVNYFVYVDYILYIATPQRVQVFDSYDDDYASVLIPGFLLNSGQLSTPEVPAEQVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D2L0</accession>
    <name>A0A2K2D2L0_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">BRADI_3g41505v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D2L0"/>
    <dbReference type="EnsemblPlants" id="PNT68503">
        <property type="protein sequence ID" value="PNT68503"/>
        <property type="gene ID" value="BRADI_3g41505v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68503">
        <property type="protein sequence ID" value="PNT68503"/>
        <property type="gene ID" value="BRADI_3g41505v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D2L0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT68503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="162" mass="18951" checksum="6C56CD1B2FAB0627" modified="2018-03-28" version="1" fragment="single">MSWIRPYAEFCSYPSWDKYDSRAHYPSHSKSSHPTYMQLQEDQQSHVIDRFNRNESVRSSKEKKEVTKQVYRIKKDVRECVVSDSISNNKEPVKLTLATKGEEMKNHIAKSEITKKVPKIKKELPLCKAKSQPGCPLGLSSWQDFFLQRLSAEELKKKNMAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D8A1</accession>
    <name>A0A2K2D8A1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted peptide</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g13205v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D8A1"/>
    <dbReference type="EnsemblPlants" id="PNT70514">
        <property type="protein sequence ID" value="PNT70514"/>
        <property type="gene ID" value="BRADI_2g13205v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70514">
        <property type="protein sequence ID" value="PNT70514"/>
        <property type="gene ID" value="BRADI_2g13205v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D8A1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036043353" description="Secreted peptide" evidence="2">
        <location>
            <begin position="19"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT70514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13501" checksum="F15FB411CFA1DDB6" modified="2018-03-28" version="1">MHYLSLLLAFLSLLVSLARLFCRHHCGPCARLPKLLPLPARFRHDRPPPAMLATGRGGKKKGSKLISSVTVMITVYCLFSFQISIAVMITFCSFFLFFTKRPMLQIPRRRTETIYKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D9S6</accession>
    <name>A0A2K2D9S6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC100846097</name>
        <name evidence="26" type="ORF">BRADI_2g22140v3</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">Bd21</strain>
            <strain evidence="27">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">Bd21</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754733.2">
        <property type="nucleotide sequence ID" value="XM_014899247.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314567.1">
        <property type="nucleotide sequence ID" value="XM_024458799.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314568.1">
        <property type="nucleotide sequence ID" value="XM_024458800.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314569.1">
        <property type="nucleotide sequence ID" value="XM_024458801.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314570.1">
        <property type="nucleotide sequence ID" value="XM_024458802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D9S6"/>
    <dbReference type="EnsemblPlants" id="PNT71042">
        <property type="protein sequence ID" value="PNT71042"/>
        <property type="gene ID" value="BRADI_2g22140v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT71043">
        <property type="protein sequence ID" value="PNT71043"/>
        <property type="gene ID" value="BRADI_2g22140v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT71045">
        <property type="protein sequence ID" value="PNT71045"/>
        <property type="gene ID" value="BRADI_2g22140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846097"/>
    <dbReference type="Gramene" id="PNT71042">
        <property type="protein sequence ID" value="PNT71042"/>
        <property type="gene ID" value="BRADI_2g22140v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71043">
        <property type="protein sequence ID" value="PNT71043"/>
        <property type="gene ID" value="BRADI_2g22140v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71045">
        <property type="protein sequence ID" value="PNT71045"/>
        <property type="gene ID" value="BRADI_2g22140v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2020077at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D9S6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000328">
        <property type="entry name" value="Leucine-rich repeat protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000453">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase HSL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000387">
        <property type="entry name" value="Probable LRR receptor-like serine/threonine-protein kinase At1g06840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974:SF108">
        <property type="entry name" value="OS05G0481100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 24" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014294255" description="non-specific serine/threonine protein kinase" evidence="24">
        <location>
            <begin position="29"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="553"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="617"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="930"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="930"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="645"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="PNT71042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="949" mass="103894" checksum="A2527C8242F2613F" modified="2018-03-28" version="1">MRTMASFGGVFWVVILVVMLPCLDVALGQSTDPSEVDALRAIKRSLLDPMNNLNNWNKGDPCTSNWTGVFCHKTNDAHLHVTELQLFKRNLSGTLAPEVSLLSQLKTLDFMWNNLTGSIPKEIGNITTLTLILLNGNQLSGFLPDEIGNLQHLNRLQVDQNQILGPIPKSFANLISVKHIHMNNNSLTGQIPAELFRLPALFHLLVDNNNLSGPLPPELGEAPSLKIFQADNNNFSGSSIPTTYNNISTLLKLSLRNCSLQGAIPDLSGISELGYLDLSWNKLTGSIPTNKLASNITTIDLSHNMLHGTVPTNFSGLPNLQLLSIEKNRLDGAVPSTIWNDIILTGNRSLVLDFQNNSLETILAVYNPPQSVTVMLYGNPVCGNSNGALIDNLCQPKSVNLQTSKQKQDSGLNCSPCPTDKDYEYNPSSSLSCFCAVPLGVGLRLKSPGITDFLPYEGTFGVNVTSLLKLFVYQLHIEHYIWEVGPRLNMQLKLFPSNTSLFNMSEVVRLRHVLAGWEITLPDMFGPYELLNFTLGSYANEYPNAASSGLSKVAFAGILAGTIAGAFALSTITTILIMRRRSRHRTVSGRSLSRFSVKIDGVRCFRFTEMARATNNFDLLAQVGQGGYGKVYKGTLDDGETVAIKRAHEDSLQGSKEFCTEIELLSRLHHRNLVSLVGYCDEEDEQMLVYEFMPNGTLRDHLSAKSKRSPGFGLRLHIALGASKGILYLHTDANPPIFHRDVKASNILLDSKFVAKVADFGLSRLAPVPDIEGTLAGHVSTVVKGTPGYLDPEYFLTHKLTDKSDVYSLGVVFLEMLTGMKPIEHGKNIVREVNKAYQSGNISEIVDSRMGLCPPDCISRFLSLATKCCEDETDARPSMSEIVRELEVILRMMPELDLVLLEAPDTYSTDMSKSLSSSSATGTYFVSQTSGTGSVDASSGVLSGVLTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DAJ1</accession>
    <name>A0A2K2DAJ1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g25925v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DAJ1"/>
    <dbReference type="EnsemblPlants" id="PNT71296">
        <property type="protein sequence ID" value="PNT71296"/>
        <property type="gene ID" value="BRADI_2g25925v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71296">
        <property type="protein sequence ID" value="PNT71296"/>
        <property type="gene ID" value="BRADI_2g25925v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DAJ1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="120" mass="13004" checksum="C36DC84FFC792138" modified="2018-03-28" version="1">MRGGALPSQERGDADVEVRREGGGHSGGTSRKRKGWRARRRRRLLLSPPGEHHWRGLEEQEVSTPGHRPPHLLPPADVHAPAPQSPPLCPPSPAPPPPEPLGLIPPLLPVQLSFVRPASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DCH0</accession>
    <name>A0A2K2DCH0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g37993v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DCH0"/>
    <dbReference type="EnsemblPlants" id="PNT71978">
        <property type="protein sequence ID" value="PNT71978"/>
        <property type="gene ID" value="BRADI_2g37993v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71978">
        <property type="protein sequence ID" value="PNT71978"/>
        <property type="gene ID" value="BRADI_2g37993v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DCH0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT71978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="187" mass="20060" checksum="E70BD75672FEA4A9" modified="2018-03-28" version="1">MLSLVPPADPPSSCATPCSMCFFFLVSGSSAGCSSATVPCPRDRHRTSPQPPFRTPTRAVAALSARARPWHVPARLRVLPARVPARRCPCLPVTSRRLPTRALACLRVPACPQPAHNRPPPARVCPPPVRAPACPRALLPTRVHPPVRAPACPSAPPCPPACPSLPTRHRLRLPKLELGMLPCSRSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DCL4</accession>
    <name>A0A2K2DCL4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prolamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_2g38530v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gliadin/glutenin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DCL4"/>
    <dbReference type="STRING" id="15368.A0A2K2DCL4"/>
    <dbReference type="EnsemblPlants" id="PNT72024">
        <property type="protein sequence ID" value="PNT72024"/>
        <property type="gene ID" value="BRADI_2g38530v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72024">
        <property type="protein sequence ID" value="PNT72024"/>
        <property type="gene ID" value="BRADI_2g38530v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DCL4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DCL4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001954">
        <property type="entry name" value="Glia_glutenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33454:SF7">
        <property type="entry name" value="ALPHA_BETA-GLIADIN MM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33454">
        <property type="entry name" value="PROLAMIN PPROL 14P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13016">
        <property type="entry name" value="Gliadin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00208">
        <property type="entry name" value="GLIADGLUTEN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0708">Seed storage protein</keyword>
    <keyword evidence="3" id="KW-0758">Storage protein</keyword>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT72024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="226" mass="25575" checksum="844CFE29ACF2380F" modified="2018-03-28" version="1">MIRDGWCHGSYKYGCTMNILLHQTILHTNTNTNTNTKSSSQHQSTMRTFLIFALLALAASTTIAQRETRCSQGMAQEKPWSVQPEQPCPHQQQDERQLQQGREQEVAPVWWRRQQQQQLYPCQDFLLQGCAAAPFQLRSQMLQQSSCRALQEQCCQQLALIPVQSRKQAIYQAVQAVFKQQLGQGQQGSLLYPQQQEQTLSTMCNNVYGPPYSPVTTSPFGVGAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DEA7</accession>
    <name>A0A2K2DEA7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Wall-associated receptor kinase galacturonan-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_2g47008v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="15368.A0A2K2DEA7"/>
    <dbReference type="EnsemblPlants" id="PNT72612">
        <property type="protein sequence ID" value="PNT72612"/>
        <property type="gene ID" value="BRADI_2g47008v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72612">
        <property type="protein sequence ID" value="PNT72612"/>
        <property type="gene ID" value="BRADI_2g47008v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="635050at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:polysaccharide binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33138">
        <property type="entry name" value="OS01G0690200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33138:SF77">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE GALACTURONAN-BINDING DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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            <end position="107"/>
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            <end position="269"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF14380"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT72612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="33446" checksum="6C6F26872397F6D2" modified="2018-03-28" version="1">MPPLLICHRLLLILLLSVAASHGDSSNDAYDSSMCLDQPYTCGGVKISYPFYLAEETKELKGYDNSYCGYPGLGIVCDGGKPTLQLDGPAKYTITSINGTIPTVSLTDPKVLGGGTDTCPRPIVGGNVTLSQGSWLFFPDSTVDYLVFLIDCSFGSGFPRPSNIDPISCQGFGGGPGLSFVLPDDEVPARNWSHACRQVIQLPVNKNIPVDHTDYRWRNSEYGKLLREGFQLGLNDSVKPQACIRCEQSNGKCGYSQGMDFIDCLCPDGRMHSDNCVKGAALSGSWPFPSHEHMSNPVVPPRAATLHLF</sequence>
</entry>
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        <lineage>
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    <reference evidence="2 3" key="1">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
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        <location>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="115" mass="13202" checksum="5EF1ED63EC11ECAA" modified="2018-03-28" version="1">MARLTWTTVHSAFNISPPNNMAHILGAWLQGIDKTLHPLILVGAAAVFWSIWLCLNDIVFYKKKIHSCMQVLLLCTNWLRLWALLQKVQHNEPMESGAKRLEWITRSLFSGLDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="BRADI_1g22887v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DKN9"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT74836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DUE3</accession>
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            <fullName evidence="1 2">Uncharacterized protein</fullName>
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        <lineage>
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            <taxon>Pooideae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DUE3"/>
    <dbReference type="EnsemblPlants" id="PNT77903">
        <property type="protein sequence ID" value="PNT77903"/>
        <property type="gene ID" value="BRADI_1g70232v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77903">
        <property type="protein sequence ID" value="PNT77903"/>
        <property type="gene ID" value="BRADI_1g70232v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DUE3"/>
    <dbReference type="OrthoDB" id="696839at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35161">
        <property type="entry name" value="OS02G0303100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35161:SF20">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT77903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="255" mass="29193" checksum="F38FA2CBFA478552" modified="2018-03-28" version="1">MCAREVLIAMCRVHFRGFSLNGKFNSSHLLFNTGKVTFDTDIAEVPFDIHSCKRDYAVIHDIFHPVFWDEENNFCPLHAEHLLHFLFTCPAGARSNSEGVIAYLTNHMALKSFMERISICTNLDGMIHRLIGQQELDFTTQVAHPYWFEQMEDVPAMWKVLSYNAGYDATGYIYNYEESVGQLMHFAKNFFTHAPNNLRMEEIEAAFSLATSESNMLPNLLQQVACQFINPPPPADFCLVKLLGDNMALLDEEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1GKI0</accession>
    <name>I1GKI0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">COP1-interacting protein-related</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100826686</name>
        <name evidence="2" type="ORF">BRADI_1g00560v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK11943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK11944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT73742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557289.1">
        <property type="nucleotide sequence ID" value="XM_003557241.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GKI0"/>
    <dbReference type="FunCoup" id="I1GKI0">
        <property type="interactions" value="1979"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GKI0"/>
    <dbReference type="EnsemblPlants" id="KQK11943">
        <property type="protein sequence ID" value="KQK11943"/>
        <property type="gene ID" value="BRADI_1g00560v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK11944">
        <property type="protein sequence ID" value="KQK11944"/>
        <property type="gene ID" value="BRADI_1g00560v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73742">
        <property type="protein sequence ID" value="PNT73742"/>
        <property type="gene ID" value="BRADI_1g00560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826686"/>
    <dbReference type="Gramene" id="KQK11943">
        <property type="protein sequence ID" value="KQK11943"/>
        <property type="gene ID" value="BRADI_1g00560v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11944">
        <property type="protein sequence ID" value="KQK11944"/>
        <property type="gene ID" value="BRADI_1g00560v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73742">
        <property type="protein sequence ID" value="PNT73742"/>
        <property type="gene ID" value="BRADI_1g00560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826686"/>
    <dbReference type="HOGENOM" id="CLU_008836_0_0_1"/>
    <dbReference type="OrthoDB" id="2020180at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GKI0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31008:SF2">
        <property type="entry name" value="COP1-INTERACTING PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31008">
        <property type="entry name" value="COP1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="671"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1040"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1138"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="720"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="735"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="781"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="816"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="883"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="894"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="917"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="932"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="984"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1050"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1085"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1147"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1183"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1204"/>
            <end position="1219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK11943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1256" mass="138888" checksum="E993625B2329D7BC" modified="2012-06-13" version="1">MRPETRLDSAAFQLTPTRTRCDLVVIANGRKEKIASGLLNPFIAHLKVAQEQIAKGGYTISLELDPETDAPWFTRGTVERFVRFVSTPEVLERVTTIESEILQLEDAIAVQGNENLGLRSVEDHNGKLVECMDGSKTSYDLDADKALILFKPDTHPAPQLQNDNGAHEENSKVQLLRVLETRKTVLRKEQAMAFARAVAAGFDIDNLGYLISFAERFGASRLMKASTQFIELWRQKHETGQWIEFEAEAMSARSEFPPFNAAGIMFMGDNMKQNLETMSVSNGDANGEDAAKADHRTPQHSGAPHEYPHGPYQSAYPPWAMHPPYPMQGMPYYPGVNPYYPPPYPPMDDSRYNHSERRASRKHSSDSKDSENLDDESDQSGSERESSHGHKSHKKGKRSSKKKPSVIVIRNVNVRSRKHGSSESESHTGSDVASEDSDDSHTKSSKRKHKRSSSKKKDGKKIILESGDEYKDEMSHGQDGDQGNWNAFQSFLLRDEEKTRESDADLFASEKEPPPPRRKESRSIDDPILLAERGSADVHEQNTTNLISANGRIRSRQMMSGDELMMPEEGQSFMDGDIKEIEAGGGGYRRRANDDFMVYGQDNSMDRGSSLDPLAEAQYKSPTLEEKTRHSVVDESFMIPVRSNSEDNLGAENRTAIDIDVELPITVQKTSDAKTEGQLFYEPEELMPERGFEDVSFGYDPAMDYDSHLQIQPDTGVENADAEDLSLCVEDEEKMPEKDKKLRSSQEGLDKRRKDASARRLSSSKGPLTDAQKRAQNLRAYKADLQKAKKEQEAEQIKRLERLKQERQKRIAARNGASNSVSTPQQAKTKPSPKISPSTYKSSKFSDAEPGSSSPLRKLPVRNTPGSDPQKTAKASKLGDGTNAVSKSTSSLTEIKKEKSGRTESSIERLKKLAEPKSNSSTDNPSNSKSASMDHPRRRSMPEDTQTKKISAIVQLDQSKSATLPELKVKSPRAPTVVAKNKTAAKETKEGPRGAKLHPTSESSGGKKSNGKVSRISNSDDNVVVEKTVVMLENEVVSTPPVILPPGRITENKTSSDDRMENPGVELEYTAIRAPPSPVDLPVDANSTIHTSDNQSNSYEVVPDYQNDEPEKPALASMEKPYEAPFARVTSLDDAASNSLPAQEAETLVRAESVRARAPEPENAVSVPEETHEKPRSKEPKGFRKLLKFGRKSHPSSAAEGTVDSDASSVDEAAAGDGSMLKNLISQDDHHSASSSKASRSFSLLSPFRKNKVIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1GKV6</accession>
    <name>I1GKV6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100846578</name>
        <name evidence="2" type="ORF">BRADI_1g01660v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562335.1">
        <property type="nucleotide sequence ID" value="XM_003562287.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GKV6"/>
    <dbReference type="STRING" id="15368.I1GKV6"/>
    <dbReference type="EnsemblPlants" id="KQK12107">
        <property type="protein sequence ID" value="KQK12107"/>
        <property type="gene ID" value="BRADI_1g01660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846578"/>
    <dbReference type="Gramene" id="KQK12107">
        <property type="protein sequence ID" value="KQK12107"/>
        <property type="gene ID" value="BRADI_1g01660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846578"/>
    <dbReference type="eggNOG" id="ENOG502QUPE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078357_0_0_1"/>
    <dbReference type="OMA" id="YFKEAFC"/>
    <dbReference type="OrthoDB" id="2020161at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019275">
        <property type="entry name" value="DUF2301"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36716">
        <property type="entry name" value="F3H9.20 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36716:SF2">
        <property type="entry name" value="F3H9.20 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10063">
        <property type="entry name" value="DUF2301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK12107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="30871" checksum="F3D0241CFE2CBC69" modified="2012-06-13" version="1">MATSTALLLPLRLAPLLPPPRFSSTPRKLLLLAPLGRSGGTRRLRAAGDRAGPLSADQTAVYNGVYGPWSVDDADIREVLLYRSGLVTAAATFLVAASGAFLPGGNAAGDAIRQSADLLYAAGAAGLGLSLLLIHIYVTPIKRFLQALWAAGVVGSVGTYAVAARPLDEGLVQFVLDHPAALWFVGPTFAALTGLVFKEGLCYGKLEAGILTFVIPILLLGHLSGLMDDGAKLGLLGVWMALFTVFAARKFQQPIKDDIGDKSVFMFNALPEDEKKSLLQKLEAQPMEQTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLR6</accession>
    <name>I1GLR6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">INO80 complex subunit B-like conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100842627</name>
        <name evidence="3" type="ORF">BRADI_1g04270v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT73923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563915.1">
        <property type="nucleotide sequence ID" value="XM_003563867.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLR6"/>
    <dbReference type="STRING" id="15368.I1GLR6"/>
    <dbReference type="EnsemblPlants" id="KQK12526">
        <property type="protein sequence ID" value="KQK12526"/>
        <property type="gene ID" value="BRADI_1g04270v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73923">
        <property type="protein sequence ID" value="PNT73923"/>
        <property type="gene ID" value="BRADI_1g04270v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842627"/>
    <dbReference type="Gramene" id="KQK12526">
        <property type="protein sequence ID" value="KQK12526"/>
        <property type="gene ID" value="BRADI_1g04270v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73923">
        <property type="protein sequence ID" value="PNT73923"/>
        <property type="gene ID" value="BRADI_1g04270v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842627"/>
    <dbReference type="eggNOG" id="ENOG502QSTQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025232_0_0_1"/>
    <dbReference type="OMA" id="EERASTC"/>
    <dbReference type="OrthoDB" id="2021186at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23021">
        <property type="entry name" value="zf-HIT_IN80B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029523">
        <property type="entry name" value="INO80B/Ies2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561:SF25">
        <property type="entry name" value="OS03G0811500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04795">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01406">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="2">
        <location>
            <begin position="390"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK12526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="530" mass="58674" checksum="0B67ED858771E350" modified="2012-06-13" version="1">MEGRGKTPLNVAEAVLKRPRSVASRKPRPKDQLASEYKDISCAASSRSISPDDDTGVEGSGHRRKELYLNGPEMRGSAEHRGEVSKKARREDRAGGDHDVGHNRSSKSKDSKHSSQGVLALACARNSGSPDNSQLPSGDTNVPPQNRVRKLKLKVSGLTRTVHTKPIQEAGEGGTPGTSDGSFHCDKQKDSGQQKHHSTNKDTQGNHIDGKHGDKHGISPPSDLVRKSKRVPKKRTLESDDEDGELRYLEKLKAAKVAPEQTIATDHSVAYDYLEDGLRKKKLSKVSKNRSAPYEVDDDFTVSQSIRDGSKKLKLGGNDDFIEEEESGRDEPKEADSPSSVKIETPGLTTRQRALHGRGGHGESVIEFPDGLPTAPSRRQGQKEKLSEVEIQAKKAEVAQRRKMQVEKAEREQQTEAMRKILGIDTEKKKEERKQKEREDKEKQARFEEYKRSCIQCVMRPEGTVITFPEKMGLPSIFNSKPISYPPPREKCAGPSCPNPYKYRDSKTKLPLCSLQCYKAVQGSNGALAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLY9</accession>
    <name>I1GLY9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100829994</name>
        <name evidence="14" type="ORF">BRADI_1g04860v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564206.1">
        <property type="nucleotide sequence ID" value="XM_003564158.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752134.1">
        <property type="nucleotide sequence ID" value="XM_014896648.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLY9"/>
    <dbReference type="STRING" id="15368.I1GLY9"/>
    <dbReference type="EnsemblPlants" id="KQK12606">
        <property type="protein sequence ID" value="KQK12606"/>
        <property type="gene ID" value="BRADI_1g04860v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK12607">
        <property type="protein sequence ID" value="KQK12607"/>
        <property type="gene ID" value="BRADI_1g04860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829994"/>
    <dbReference type="Gramene" id="KQK12606">
        <property type="protein sequence ID" value="KQK12606"/>
        <property type="gene ID" value="BRADI_1g04860v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12607">
        <property type="protein sequence ID" value="KQK12607"/>
        <property type="gene ID" value="BRADI_1g04860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829994"/>
    <dbReference type="eggNOG" id="KOG0335">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_16_3_1"/>
    <dbReference type="OMA" id="ISGNDRW"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GLY9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17967">
        <property type="entry name" value="DEADc_DDX3_DDX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000397">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044763">
        <property type="entry name" value="Ded1/Dbp1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="176"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="207"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="418"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="93"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="572"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="176"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="600"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK12606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="637" mass="67364" checksum="2EA93CF539F68B9B" modified="2012-06-13" version="1">MRSSWADSVANAEESAPATAAASAAVANHQNSRPTRSSYVPPHLRGRSPGPPSENHAPGLAPAPAGIPPSGAVQPSGYAAVVGGGSRWAAPPAGGGTGVGVVRQGGGRGGGGGGGGGGWNSRPGLDRRDREPNPFGDVEPPAEVDFESQANTGINFDAYEDIPVETSGHDVPTPVNTFAEIDLGDALNENIRRCKYVRPTPVQRHAIPIVIGGRDLMACAQTGSGKTAAFCFPIISGIMKSRPPQRPRGSRTAYPLALILSPTRELSVQIHEEAKKFAYQTGVRAVVAYGGAPIHQQLRELERGVEILVATPGRLMDLLERARVSLQMVNYLALDEADRMLDMGFEPQIRKIVEQMDMPPRGVRQTMLFSATFPKEIQRLASDFLADYIFLAVGRVGSSTDLIAQRVEFVLEADKRSYLMDLIHAQKANTVPGKQSLTLVFVETKRGADALENWLYTNGFPATSIHGDRTQQEREYALRSFKSGATPILVATDVAARGLDIPDVAHVINFDLPNDIDDYVHRIGRTGRAGKSGVATAFFNEGNMSLARPLCELMQEANQEVPQWLERYSARSSFGGGGGRNRRSGGARFGGRDFRRDRGAGGYGGGGGGGGGGYGGGGYGGGGGGYGGGQGASSSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMG6</accession>
    <name>I1GMG6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC100824738</name>
        <name evidence="24" type="ORF">BRADI_1g06280v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">Bd21</strain>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312436.1">
        <property type="nucleotide sequence ID" value="XM_024456668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMG6"/>
    <dbReference type="EnsemblPlants" id="KQK12834">
        <property type="protein sequence ID" value="KQK12834"/>
        <property type="gene ID" value="BRADI_1g06280v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824738"/>
    <dbReference type="Gramene" id="KQK12834">
        <property type="protein sequence ID" value="KQK12834"/>
        <property type="gene ID" value="BRADI_1g06280v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUMK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_116_2_1"/>
    <dbReference type="OMA" id="THNDKES"/>
    <dbReference type="OrthoDB" id="643280at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GMG6">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000227">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF19">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093964" description="Receptor-like serine/threonine-protein kinase" evidence="20">
        <location>
            <begin position="23"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="450"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="22">
        <location>
            <begin position="24"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="23">
        <location>
            <begin position="347"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="507"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="535"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQK12834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="805" mass="89368" checksum="86B2DA8A15950626" modified="2012-06-13" version="1">MRVLPVVCILLFFCSTTPASFAATDTILAGQALDINDKLVSKNGRYALGFFKGRGKSSESTTNWYLGIWFNTVRKFTSAWVANRDKPIKNTTSLEFTLSIDGNLVILNPSTKSIIWSTTAKNRRNSTIAMLSNSGNLILTDYSNSSEVLWQSFDHPTDTFFPGAKLGLDKVTGLNRRIVSWKNLVNPASGAYHFELDPSGINQLLLLSLNLSVPYWSSGVWNGKYFASIPEMTSDHPIFSSTFVDNDQEKYFTYNLVHENMVTRHVIDVSGQTKTFIWLEDSQDWTMIYAQPKAQCDVYAICGAFTTCTDNVVPHCNCMEGFTITSPEDWELEDRSGGCSRITQLDCTSNKSTTHTTDKFYSVPCVRSPQDNPKVEAARSAGECAQVCLNNCSCTAYSFGYSGCSIWYNELLNVRQLQCSDTANSSEETIFLRLSAKDVQSLKNNRRGTVVGAVTGAGIFVLCLLALILSLMIWRNKRKSSGHISNVFQGCSGIIAFRYNDLQGATKKFTDKLGEGSFGSVFKGFINDSIAIAVKRLDGAYQGEKEFRAEVNSIGVIQHINLVNLVGFCCDGSKRLLVYEYMPNHSLDVHLFRGNSMVLNWTARYRIALGVARGLAYLHESCRDCIIHCDIKPENILLDASFLPKIADFGMAKLLGRDFSRVLTTMRGTAGYLAPEWISGVAITPKVDVYSYGMVLLEIISGRRNTCTTCSSNDNLDVYFPVHAAHKLLEGDMESLLDHMLHGDVNLDEAELVCKVACWCIQDDEFDRPTMGEVVQILEKLVEIRMPPIPRLLQAMAGSSSSICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPJ9</accession>
    <accession>A0A0Q3NAK5</accession>
    <name>I1GPJ9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="19 20">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BRADI_1g12410v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13774.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPJ9"/>
    <dbReference type="EnsemblPlants" id="KQK13774">
        <property type="protein sequence ID" value="KQK13774"/>
        <property type="gene ID" value="BRADI_1g12410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13774">
        <property type="protein sequence ID" value="KQK13774"/>
        <property type="gene ID" value="BRADI_1g12410v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWDY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_35_2_1"/>
    <dbReference type="InParanoid" id="I1GPJ9"/>
    <dbReference type="OMA" id="WYTECAS"/>
    <dbReference type="OrthoDB" id="779887at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000142">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000002">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000343">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1050">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003641787" evidence="16">
        <location>
            <begin position="21"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="18">
        <location>
            <begin position="22"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="18">
        <location>
            <begin position="132"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="345"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQK13774.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="653" mass="70932" checksum="8D2CADFBBFA58197" modified="2012-06-13" version="1">MRPMRSLLSLLLLLVVAANADDLILGDCPSNTNYTLGGVFHANLDALLSSLPAATAAADGFSKNTTGAAAPDKAYGLAQCRADVNASDCRTCLDASVRDMASKCPGLKDATRIYEKCLLRHDNASFFGVLDTSLHVYMNSYVNATQRERFTLLLGTLMNNLTHRAAYASPRMFAVGMTSLGPLVTIYGMAECIRDLPADDCNSCLVDAVSRIPSCCDGKQGGRVIFRSCSVRFEGYPFYDVQAAEAAMLSPPPAGSGRVDGSDNSAPTSTGSSGIVRTALSVSIPVAAVVLVLLVTAFCLLSKRNRKPHKHVPIATADNVNGDEMMSLESLLYDLNTLRAATDNFSEDNKLGEGGFGPVYKDGHEIAVKRLSTTSQQGHLEMKNEVLFLAKLQHKNLVRLLGCCIEEDEKLLVYEFLSNKSLDKILFDPGRQQELSWANRHKIIQGIGRGLLYLHEDSRLTIIHRDLKASNILLDPDINPKISDFGLAKLFTMDESVGNTSRIAGTYGYMAPEYAFHGTFSAKSDVFSYGVLVLEVVTGRRNAYRQDSEDLVSSVWRHWSRGNMSGLLESCSADGLRPQEMLRCIHVGLLCVQEDAHLRPGMAAVVVMLNSRSITLPAPTAPAFIVPSRLVARTKVVRAPSINDASVSDLEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWG6</accession>
    <name>I1GWG6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100830907</name>
        <name evidence="6" type="ORF">BRADI_1g33540v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thaumatin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563464.1">
        <property type="nucleotide sequence ID" value="XM_003563416.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWG6"/>
    <dbReference type="EnsemblPlants" id="KQK17299">
        <property type="protein sequence ID" value="KQK17299"/>
        <property type="gene ID" value="BRADI_1g33540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830907"/>
    <dbReference type="Gramene" id="KQK17299">
        <property type="protein sequence ID" value="KQK17299"/>
        <property type="gene ID" value="BRADI_1g33540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830907"/>
    <dbReference type="eggNOG" id="ENOG502QQ9U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043181_0_1_1"/>
    <dbReference type="OMA" id="YNRCGET"/>
    <dbReference type="OrthoDB" id="430315at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09218">
        <property type="entry name" value="TLP-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000002">
        <property type="entry name" value="Thaumatin-like protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094153" description="Thaumatin-like protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="31"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="151"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="156"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK17299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="25412" checksum="724AC602EC9BD61E" modified="2012-06-13" version="1">MATVAGLVSILALLLAAREASAATMALYNRCGETVWPGIQPGAGKEILARGGLQLLPNRATSIRLPAGWSGRVWGRQGCSFDAAGRGKCATGDCGGALYCNGAGGAPPATLAEITLGASGSTSQDFYDVSLVDGYNIPIAMTPYHGSGTNCLPAGCVSDLNRVCPAGLAVRGGGRVVGCRSACAAYGSPQYCCTGQFGGPQTCKPTAYSKLFKQACPKAYSYAYDDPTSILTCAPGTSYVVTFCPHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXT8</accession>
    <name>I1GXT8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">1-aminocyclopropane-1-carboxylate oxidase</fullName>
            <ecNumber evidence="10">1.14.17.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Ethylene-forming enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100831517</name>
        <name evidence="16" type="ORF">BRADI_1g37420v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 = ethene + L-dehydroascorbate + hydrogen cyanide + CO2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:23640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:18153"/>
            <dbReference type="ChEBI" id="CHEBI:18407"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:58360"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.14.17.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-methionine; ethylene from S-adenosyl-L-methionine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.17.4" evidence="10"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563715.1">
        <property type="nucleotide sequence ID" value="XM_003563667.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXT8"/>
    <dbReference type="STRING" id="15368.I1GXT8"/>
    <dbReference type="EnsemblPlants" id="KQK17895">
        <property type="protein sequence ID" value="KQK17895"/>
        <property type="gene ID" value="BRADI_1g37420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831517"/>
    <dbReference type="Gramene" id="KQK17895">
        <property type="protein sequence ID" value="KQK17895"/>
        <property type="gene ID" value="BRADI_1g37420v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831517"/>
    <dbReference type="eggNOG" id="KOG0143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010119_16_1_1"/>
    <dbReference type="OMA" id="YPQCPHP"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009815">
        <property type="term" value="F:1-aminocyclopropane-1-carboxylate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009693">
        <property type="term" value="P:ethylene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009835">
        <property type="term" value="P:fruit ripening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000010">
        <property type="entry name" value="1-aminocyclopropane-1-carboxylate oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0266">Ethylene biosynthesis</keyword>
    <keyword evidence="8" id="KW-0292">Fruit ripening</keyword>
    <keyword evidence="7 14" id="KW-0408">Iron</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="15">
        <location>
            <begin position="145"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK17895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="293" mass="33382" checksum="FD7B215B259FA699" modified="2012-06-13" version="1">MEIPVIKMDELHGKKRSATLSLLHDACAQWGFFWLENHGVNDELMDKIKSLVNKHYEQDIEKNFYSSEMAKTLGSDKGASNVDWECSCMYHHQPESNIHDIPQLLRTTLPEYAEEVVKLAERLAEVMSENLGLDKDYLKKAFSKPSVGIKVAKYPRCNHPELVMGLRGHTDAGGIILLFQDDLVPGLEFKKDGRWIPIPPTEGNRIFVNLGDQIEVMSNGIYKSICHQVLPNKNGSRLSIATFYNPGADAIIFPAPKLTYPSQYRFQDYLNFYSTSKFSDKVSRFEITKTVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1GY07</accession>
    <name>I1GY07_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100844248</name>
        <name evidence="18" type="ORF">BRADI_1g37940v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="5"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310760.1">
        <property type="nucleotide sequence ID" value="XM_024454992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GY07"/>
    <dbReference type="STRING" id="15368.I1GY07"/>
    <dbReference type="EnsemblPlants" id="KQK17994">
        <property type="protein sequence ID" value="KQK17994"/>
        <property type="gene ID" value="BRADI_1g37940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844248"/>
    <dbReference type="Gramene" id="KQK17994">
        <property type="protein sequence ID" value="KQK17994"/>
        <property type="gene ID" value="BRADI_1g37940v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2245">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="SKANWSA"/>
    <dbReference type="OrthoDB" id="412748at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GY07">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.590:FF:000002">
        <property type="entry name" value="Nuclear poly(A) polymerase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007010">
        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF22">
        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04926">
        <property type="entry name" value="PAP_RNA-bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="17">
        <location>
            <begin position="6"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="16">
        <location>
            <begin position="202"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="15">
        <location>
            <begin position="349"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="15">
        <location>
            <begin position="409"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="476"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="735"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="560"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="574"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="589"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="605"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="739"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK17994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="755" mass="83882" checksum="370B842FFA566625" modified="2012-06-13" version="1">MTTSNKPLSLAGPVDADIQRTAELNKFLVEAGLYESAHESARREEVLGELDKIVKDWVKQLTSQRGYTDQMVEDANAVLFTFGSYRLGVHGPGADVDTLCVGPSYVNREEDFFIVLHDILAQTEEVTELQPVPDAHVPVMKFKFHGISIDLLYASVSLLVVPSDLDISQESVLYDIDEATVRSLNGCRVADQILRLVPNIENFRTTLRCLKHWAKRRGVYSNVTGFLGGVNWALLVARVCQLYPNAVPSMLVSRFFRVFTQWRWPNPVMLCAIEEEELGFPVWDPRKNPRDRTHHMPIITPAYPCMNSSYNVSTSTLRVMIEQFQFGNKICQEIELNKANWPALFESFQFFEAYKNYLQVDIIAEDDEDLRLWKGWVESRLRQLTLKIERDTYGKLQCHPYPYEYADPSRQCAHCAFFMGLSRNVGVKIEEGQQFDIRGTVDEFRHDINMYMFWKPGMELAVSHVRRKEIPAYVFPEGYRRPRPQRHVSHQQQSDKNDTENGNMTGSPDGQLKRKHDSAGTDDTEPCRSVKRASVSPVHPKHDSPGTDDTEPCRSVKRASVSPVHPTTSSPSSGNISDEATCNNQKKMSSNASGGSQNSHGSGNLEQANCSGSSPASEKSTDSIASDSKCVIGETVCSGDVTNNLVTCISPVNNSTPTVAVCTTLKRVAEKVVSELVGSESLGGNNPELLEIAEKDMGSVLVENVHFGGNGVTQSGLHEELEPNNGIEVLSKAHAGVNSDGSQKPSMRVSLTSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZR0</accession>
    <name>I1GZR0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100846363</name>
        <name evidence="1" type="ORF">BRADI_1g45680v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560913.1">
        <property type="nucleotide sequence ID" value="XM_003560865.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZR0"/>
    <dbReference type="STRING" id="15368.I1GZR0"/>
    <dbReference type="EnsemblPlants" id="KQK18972">
        <property type="protein sequence ID" value="KQK18972"/>
        <property type="gene ID" value="BRADI_1g45680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846363"/>
    <dbReference type="Gramene" id="KQK18972">
        <property type="protein sequence ID" value="KQK18972"/>
        <property type="gene ID" value="BRADI_1g45680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846363"/>
    <dbReference type="eggNOG" id="ENOG502QPJ9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061278_1_0_1"/>
    <dbReference type="OMA" id="SEYQKNT"/>
    <dbReference type="OrthoDB" id="741151at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010683">
        <property type="entry name" value="DUF1262"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31050:SF3">
        <property type="entry name" value="OS08G0412800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31050">
        <property type="entry name" value="OS08G0413200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06880">
        <property type="entry name" value="DUF1262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK18972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="340" mass="37891" checksum="B33981E607009CFB" modified="2012-06-13" version="1">MYATWPLQLFKSNPKAASWPPPDGGNSGYLVATDGEDEEEGMSCMGMGGTAVHDLPFPQNRLITISDADSSETVLFMPVLDQPLSSNRYYAVIASGRKKGLVRTCCRESELSHGCFTRFNHAEPRAFDPADVYQQMEIIQRRRGQFTARAVAADGFPYSLYSNKYWHARASRPMNYTLDLGEAIGLNAALIRSRQPDDAFRAVGRWYCPFFHVKEGGVSPAEQMKRAAYYGVTLEQRWEEPVSSEKLPSFRALIGGSLEARQESSVGWSGYVSFRAPAGQRVGVSTSVWQRMRFEQRNGARADAARGTGRSVPVERFVLKRTDGSVAVAFDFLRAAQHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1H0Q0</accession>
    <name>I1H0Q0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100846669</name>
        <name evidence="3" type="ORF">BRADI_1g48360v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK19459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003561002.4"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQK19459">
        <property type="protein sequence ID" value="KQK19459"/>
        <property type="gene ID" value="BRADI_1g48360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846669"/>
    <dbReference type="Gramene" id="KQK19459">
        <property type="protein sequence ID" value="KQK19459"/>
        <property type="gene ID" value="BRADI_1g48360v3"/>
    </dbReference>
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    <dbReference type="eggNOG" id="KOG1947">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FBXL15_LRR"/>
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        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
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        <property type="entry name" value="GH16156P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
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    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="10"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="2">
        <location>
            <begin position="76"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK19459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="24529" checksum="E2C176267786B9B1" modified="2012-06-13" version="1">MAKEEAPHVERLPADLLAHVLSLLPSFHDLAMAGGVSQRWHQAVGRSLSSRRRLSFAGQRTGDDFTARLVRAAVNLRDLDISRSCWGCQITDQGLLKLSSATCVPNLTSISLWGLARITDKGVVHLISRARSLQHLNIGGTFITDESLYAVANSCANLKSIIVWSCRHVTEAGLVAVVRRCPELECINVGGMRVSPESFAGLVSISPSLRIRSIPQILNADVLQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2K6</accession>
    <name>I1H2K6_BRADI</name>
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        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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        <name evidence="5" type="ORF">BRADI_1g53830v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557374.1">
        <property type="nucleotide sequence ID" value="XM_003557326.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2K6"/>
    <dbReference type="STRING" id="15368.I1H2K6"/>
    <dbReference type="EnsemblPlants" id="PNT76814">
        <property type="protein sequence ID" value="PNT76814"/>
        <property type="gene ID" value="BRADI_1g53830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826687"/>
    <dbReference type="Gramene" id="PNT76814">
        <property type="protein sequence ID" value="PNT76814"/>
        <property type="gene ID" value="BRADI_1g53830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826687"/>
    <dbReference type="eggNOG" id="KOG0027">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094378_1_0_1"/>
    <dbReference type="OMA" id="AMECQGC"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000928">
        <property type="entry name" value="Calcium-binding allergen Ole e 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
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    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
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        <property type="entry name" value="EF_hand_dom"/>
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    <dbReference type="InterPro" id="IPR039647">
        <property type="entry name" value="EF_hand_pair_protein_CML-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10891">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
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        <property type="match status" value="2"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="122"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT76814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="193" mass="21314" checksum="A2B2E07F0B83DF00" modified="2012-06-13" version="1">MWRMVMAAHNITIALVSSLLMLILGPLIIDVISVSKKIGRFLCLATKFLADGDSNAIDNVTLSDSPLPAAQLAQGGGLMCTDIEHVTARLGLAARGFGETIGKCYECDAMSAIDELMDDKEASEDELKEAFYVFDRDEDGFICSGELWNVMRRLGWKEGARHEDCVRMIHAFDEDGDGKISFLEFTHMMENAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6D6</accession>
    <name>I1H6D6_BRADI</name>
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        <recommendedName>
            <fullName evidence="6">PLAC8 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100834377</name>
        <name evidence="3" type="ORF">BRADI_1g64970v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228728.1">
        <property type="nucleotide sequence ID" value="XM_010230426.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316118.1">
        <property type="nucleotide sequence ID" value="XM_024460350.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316128.1">
        <property type="nucleotide sequence ID" value="XM_024460360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6D6"/>
    <dbReference type="STRING" id="15368.I1H6D6"/>
    <dbReference type="EnsemblPlants" id="PNT77556">
        <property type="protein sequence ID" value="PNT77556"/>
        <property type="gene ID" value="BRADI_1g64970v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77557">
        <property type="protein sequence ID" value="PNT77557"/>
        <property type="gene ID" value="BRADI_1g64970v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77558">
        <property type="protein sequence ID" value="PNT77558"/>
        <property type="gene ID" value="BRADI_1g64970v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77559">
        <property type="protein sequence ID" value="PNT77559"/>
        <property type="gene ID" value="BRADI_1g64970v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834377"/>
    <dbReference type="Gramene" id="PNT77556">
        <property type="protein sequence ID" value="PNT77556"/>
        <property type="gene ID" value="BRADI_1g64970v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77557">
        <property type="protein sequence ID" value="PNT77557"/>
        <property type="gene ID" value="BRADI_1g64970v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77558">
        <property type="protein sequence ID" value="PNT77558"/>
        <property type="gene ID" value="BRADI_1g64970v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77559">
        <property type="protein sequence ID" value="PNT77559"/>
        <property type="gene ID" value="BRADI_1g64970v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834377"/>
    <dbReference type="eggNOG" id="ENOG502RHKK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018900_1_0_1"/>
    <dbReference type="OMA" id="VPPLMQM"/>
    <dbReference type="OrthoDB" id="6407410at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009975">
        <property type="term" value="F:cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051762">
        <property type="term" value="P:sesquiterpene biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021369">
        <property type="entry name" value="DUF2985"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31045:SF19">
        <property type="entry name" value="OS03G0299800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31045">
        <property type="entry name" value="PLAC8 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11204">
        <property type="entry name" value="DUF2985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT77556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="64237" checksum="C6C86BDC259489E0" modified="2012-06-13" version="1">MVPHEASSSRHEIVEVLGEPGEPSSPGTMRLIDFIPVYIPTVERGALSRSARKSRFLDFLRARPSKDWFLRSNFVGRLRRRNQTSSSSSVEEDRESHGINAGVGGVECRPRTRRRFRVPFVRKIKWGKLWTYTVSWCRQPANFGMIIWLAFVAAGLLLLFLLMTGLLDEAIPKEERRKKWTEVTNQILNALFTIMCLYQHPKIFHHLVLLLRWRAGPGGDRAEVREAYCKEGAPRPHDRAHMLVVVALLHVTCLAQYFCCALYWSYSSTDRPDLPLNIGNGLGTGVPVVAVLYVAYSPLGRRQANEPDTELSSVSAGGRQTRTDHQNDGEDVEIRIYNRSVVVSSPEWSGGLCDCCDDGTVCALSAACTCCVFGWNMERLGFGNMYVHAFTFILLCVAPFFTFSVTALNIHDDDIRDAVVAAGVLLGFCGFLYGGYWRTQMRKRYKLPPAAAAASGDGGRCWAATGDCAKWLFCWSCALAQEVRTANFYDVEDDRFVVQYGARDEEGRAVLVPLPREATHSRSLSCPPKMGAMISSLMDVEMAGVAAMERSATYSGNHHAMRPPLPPLIQMDRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1H968</accession>
    <name>I1H968_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100846343</name>
        <name evidence="9" type="ORF">BRADI_1g73110v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558704.1">
        <property type="nucleotide sequence ID" value="XM_003558656.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H968"/>
    <dbReference type="FunCoup" id="I1H968">
        <property type="interactions" value="2505"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H968"/>
    <dbReference type="MEROPS" id="S59.A01"/>
    <dbReference type="EnsemblPlants" id="KQK23395">
        <property type="protein sequence ID" value="KQK23395"/>
        <property type="gene ID" value="BRADI_1g73110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846343"/>
    <dbReference type="Gramene" id="KQK23395">
        <property type="protein sequence ID" value="KQK23395"/>
        <property type="gene ID" value="BRADI_1g73110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846343"/>
    <dbReference type="eggNOG" id="KOG0845">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012427_0_0_1"/>
    <dbReference type="OMA" id="RWKFEVD"/>
    <dbReference type="OrthoDB" id="3797628at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.690:FF:000002">
        <property type="entry name" value="Nuclear pore complex protein NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1610.10">
        <property type="entry name" value="Peptidase S59, nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036903">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021967">
        <property type="entry name" value="Nup98_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF26">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04096">
        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12110">
        <property type="entry name" value="Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82215">
        <property type="entry name" value="C-terminal autoproteolytic domain of nucleoporin nup98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase S59" evidence="8">
        <location>
            <begin position="12"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK23395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1007" mass="113491" checksum="392D45E788CEF2AB" modified="2012-06-13" version="1">MALDAMFPVLRSSDYFTRPSIDELVEREAADPGYCSRVPNFVVGRARYGQVMFSGNTDVRGLDLNEIVTFDRHCVVVYGDEAGKPAVGHGLNKAAEVMLLLDLRSLPEPSILDELLRCRTKKQGARFLSFNPVNGYWKFEVDHFSRFGLVDEEEEDVAMDEVAARQPISEARDPPSNGYELELSHSLPAHLGLDPAKMQEMRMALFPNDEDDVDMEDGFPSDQRYLSRERMSVDSPNTSAKGARLRSLSPLQGSSQKFGRRSGMLARKEPHALLEYSVNPSELGPSSHGILMSGKNKGFPVRMAKVEGFKLPAEQATPVAGKVYSNCVVDAALFMGRSFRVGWGPNGILIHSGSLVNSPGTGLSSVIHIEKVAGDKVVRDEQNKVKEELAELCFSDPMDLHRRLDREVLETESDSFKLKLQKVVASRLVLPEICRSYIDIIERQLEISDLSMSLRVLLMHQVTVWELIRVLFSEKTAGNQLEFSGDEDQEGMIIDKKEVSVNIDLEALPLVRRADFSNWLQDSICHRVQGEVGSLSDARYLEHIILLLTGRQLDTATEIAASRGDVRLAILLSQAGGSMLNRSDVAQQLDLWKINGLDFDYIEEDRLRIYELLAGNIQGALLDSPIDWKRYLGLIMWYQLSPDTSLDIIIHSYHQLLGEGKVPNPVPVYIDEGPLEEAVQWSPGDRFDLSFYLMLLHANQDEKFGLLKTMFSAFSSSYDPLDYHMIWHQRSILEAIGAFSANDLHVLDLSFVHQLLCLGKCHWAIYVILHMPHLDDAPYIHEKLIKEILSQYCELWSKDVAQRQYITELGIPAEWIHEALALFHEYYGDRQGALENYIQCGNWKKAHTIFMTSVAHSLFLSSNHQEIWGITNVLENHKSEIADWDIGAGIYIDYFIIKNSMQEESTMDDDDSDPLERKNESCKSFFGRLNDSLHIWGSKLPVEARACFSKMAEELSQLLMNSPGEGLGPDLYMGCFHTMLNAPVPNDHKASYLQEAVSVFTDILCGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HBH9</accession>
    <name>I1HBH9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100827574</name>
        <name evidence="7" type="ORF">BRADI_2g01580v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT69857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567743.1">
        <property type="nucleotide sequence ID" value="XM_003567695.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754177.1">
        <property type="nucleotide sequence ID" value="XM_014898691.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HBH9"/>
    <dbReference type="STRING" id="15368.I1HBH9"/>
    <dbReference type="EnsemblPlants" id="KQK02460">
        <property type="protein sequence ID" value="KQK02460"/>
        <property type="gene ID" value="BRADI_2g01580v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK02463">
        <property type="protein sequence ID" value="KQK02463"/>
        <property type="gene ID" value="BRADI_2g01580v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69857">
        <property type="protein sequence ID" value="PNT69857"/>
        <property type="gene ID" value="BRADI_2g01580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827574"/>
    <dbReference type="Gramene" id="KQK02460">
        <property type="protein sequence ID" value="KQK02460"/>
        <property type="gene ID" value="BRADI_2g01580v3"/>
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        <property type="protein sequence ID" value="KQK02463"/>
        <property type="gene ID" value="BRADI_2g01580v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69857">
        <property type="protein sequence ID" value="PNT69857"/>
        <property type="gene ID" value="BRADI_2g01580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827574"/>
    <dbReference type="eggNOG" id="KOG1609">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061276_0_0_1"/>
    <dbReference type="OMA" id="GECRICH"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HBH9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF3675"/>
    </dbReference>
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        <property type="entry name" value="MARCH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012:SF174">
        <property type="entry name" value="OS01G0121200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12428">
        <property type="entry name" value="DUF3675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="45"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="230"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="242"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK02463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="271" mass="31117" checksum="3B1A889FEB99BA17" modified="2012-06-13" version="1">MGDHLALLVDRLLTESTLEAAIGNNNRKLQAAETVEYCCEATALLPASKMVECRICQEEDWDNSMEAPCSCRGSLKYAHRKCIQRWCNEKGDTVCEICLQQFKPGYTSPEQLFHYGSIPMNFRGNWEIARQDLHDSQVITMVPSERDFIDEYEDYLPIRTRSSALCCRTIAIIFMALLILRHTLPLMIGGNGEYSFALFSLLVLRTAGILFPILVMVRALATYHRRRRQQENQETYMSSSDNDNDEEEAEEEDTDTDSAWLHTQPRLVPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HBL8</accession>
    <name>I1HBL8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bowman-Birk serine protease inhibitors family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100827050</name>
        <name evidence="9" type="ORF">BRADI_2g01927v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the Bowman-Birk serine protease inhibitor family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565286.1">
        <property type="nucleotide sequence ID" value="XM_003565238.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HBL8"/>
    <dbReference type="FunCoup" id="I1HBL8">
        <property type="interactions" value="202"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK02511">
        <property type="protein sequence ID" value="KQK02511"/>
        <property type="gene ID" value="BRADI_2g01927v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827050"/>
    <dbReference type="Gramene" id="KQK02511">
        <property type="protein sequence ID" value="KQK02511"/>
        <property type="gene ID" value="BRADI_2g01927v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827050"/>
    <dbReference type="eggNOG" id="ENOG502R3JY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059102_2_0_1"/>
    <dbReference type="OMA" id="WPGPSCT"/>
    <dbReference type="OrthoDB" id="757405at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00023">
        <property type="entry name" value="BBI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.69.10">
        <property type="entry name" value="Cysteine Protease (Bromelain) Inhibitor, subunit H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035995">
        <property type="entry name" value="Bowman-Birk_prot_inh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000877">
        <property type="entry name" value="Prot_inh_BBI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33479">
        <property type="entry name" value="BOWMAN-BIRK TYPE BRAN TRYPSIN INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33479:SF22">
        <property type="entry name" value="BOWMAN-BIRK TYPE BRAN TRYPSIN INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00228">
        <property type="entry name" value="Bowman-Birk_leg"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00269">
        <property type="entry name" value="BowB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57247">
        <property type="entry name" value="Bowman-Birk inhibitor, BBI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 6" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094445" description="Bowman-Birk serine protease inhibitors family domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Bowman-Birk serine protease inhibitors family" evidence="8">
        <location>
            <begin position="51"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Bowman-Birk serine protease inhibitors family" evidence="8">
        <location>
            <begin position="134"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="site" description="Reactive bond for trypsin" evidence="5">
        <location>
            <begin position="59"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="site" description="Reactive bond for trypsin" evidence="5">
        <location>
            <begin position="142"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600877-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK02511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="195" mass="21305" checksum="633DE59EA1D4A8C1" modified="2012-06-13" version="1">MKGRVATVLVLTLSLAAAWLSAADDVDTIRLPSHANEVMAKASAEKRPWKCCNRARCTRSIPPFCWCHDVVERCSGACKDCREAEREDESDPEGYVCHDMYHGSPGPACSSGTDDNKAVAADETAAKKKRPWKCCDRTLCTKSAPPTCSCLDKVERCSGRCKRCGPSESDPSGLFCLDRYHGWPGPKCTNKNDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCW6</accession>
    <name>I1HCW6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100840296</name>
        <name evidence="12" type="ORF">BRADI_2g05760v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564752.1">
        <property type="nucleotide sequence ID" value="XM_003564704.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCW6"/>
    <dbReference type="FunCoup" id="I1HCW6">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HCW6"/>
    <dbReference type="EnsemblPlants" id="KQK03131">
        <property type="protein sequence ID" value="KQK03131"/>
        <property type="gene ID" value="BRADI_2g05760v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK03131"/>
        <property type="gene ID" value="BRADI_2g05760v3"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="entry name" value="Myb_dom_plants"/>
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        <property type="entry name" value="Plant_Stress_Dev_TF"/>
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        <property type="entry name" value="Znf_CCHC"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="OS04G0341900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44191">
        <property type="entry name" value="TRANSCRIPTION FACTOR KUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Homeodomain-like"/>
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        <property type="entry name" value="HTH_MYB"/>
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        <property type="entry name" value="MYB_LIKE"/>
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        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="3"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="11">
        <location>
            <begin position="116"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="116"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="10">
        <location>
            <begin position="124"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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            <begin position="258"/>
            <end position="279"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="18"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50090"/>
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        <source>
            <dbReference type="EMBL" id="KQK03131.1"/>
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    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="279" mass="29160" checksum="F2C512F946F96B7D" modified="2012-06-13" version="1">MARKCSSCGHNGHNSRTCSGHRGMESGGLRLFGVQLHVGSAAAASPLKKSFSMDCLSSSSSGSAYYAAAAAAAVAASNSASTSVSSASSSLVSVEESGEKMGHGGYLSDGLMGRAQERKKGVPWTEEEHRRFLAGLEKLGKGDWRGISRHFVTTRTPTQVASHAQKYFLRQGGLAHKKRRSSLFDVVENGGGTALKDEASSVVSVEGLGLFPALSLGGAPSVTGHHAPKPPAPFRVANASASPWQAPDLELKIATAARKAEDRPGPSRRTPFFGQIRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJN9</accession>
    <name>I1HJN9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100824176</name>
        <name evidence="11" type="ORF">BRADI_2g26100v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231402.1">
        <property type="nucleotide sequence ID" value="XM_010233100.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJN9"/>
    <dbReference type="STRING" id="15368.I1HJN9"/>
    <dbReference type="EnsemblPlants" id="KQK06383">
        <property type="protein sequence ID" value="KQK06383"/>
        <property type="gene ID" value="BRADI_2g26100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824176"/>
    <dbReference type="Gramene" id="KQK06383">
        <property type="protein sequence ID" value="KQK06383"/>
        <property type="gene ID" value="BRADI_2g26100v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824176"/>
    <dbReference type="eggNOG" id="KOG0239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007631_2_1_1"/>
    <dbReference type="OMA" id="HIWLVDL"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01366">
        <property type="entry name" value="KISc_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF2">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-14S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="196"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="598"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="768"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="598"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="769"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="782"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="279"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK06383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="862" mass="95117" checksum="BC75BB3164658795" modified="2012-06-13" version="1">MEAYPGTSSPSPQSPTAAASPPPQPQGEVECGAVEFSDHLSPSRHAGPADSNTAPPPSPASVAPMPQNLEGEETVPTSEEVVDEEAAVEELAKEGDEAVAGGEGEALRIFLEELGDQADDCLIPSPRLKEIASPDRLAALQVLGGRYNSLVEKFKLQVAKCVEECAPRFDGLKKKYTVECAERRRLYNELIELRGNIRVFCRCRPLSADEVSRGCSSVIDVDPSQETELQYVPSEKERKNFKFDHVFGPADDQEAVFAESLPVVRSVMDGFNVCIFAYGQTGTGKTFTMEGVPENRGVNYRALEELFRMSNERSSSVAYTFYVSILEVYNEKIRDLLDDNCEQASKRLDIKQSADGAQEVPGLVEAPIYTIDGVWDKLKAGAKNRSVGSTSVNELSSRSHSLVRVTVRSEHLVTGEMSRSHMWLVDLAGSERLAKTEVEGERLKESKFINKSLSALGDVIAALASKNSHIPYRNSKLTHLLQSSLGGDCKTLMFVQISPSSTDSGETLCSLNFASRVRAIEHGPARKQADPAENFKLKQMTEKLCHEEKENVKLNESLQLMQLKYASRENVFRTLQDKIRETEQACRTHQQRVRELENELANEKKAARDTVRPTKPSLAAPVRQRPPLAPMRQRPPLVRISNQAPPPSGPSRQRLGSKGPSVQNKENIPVLDKAAGKARRVSLVPMMRQIPLQPKRRSSIAILPSERERMSVLPEKRAMSRLSHMQMPKTTRPLANFNPIPGTPVAASKAPVDATPDVRGRFKRIELGSSSKFSSPPMQSMWKSRNNVSSPQQRLRLQSGSGNASKLCFSIHKKVGLGSPARPRNSLMSGTGIFDPALREQMMAGKFGNAQRVFSKRRMSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HKA2</accession>
    <name>I1HKA2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">intramembrane prenyl-peptidase Rce1</fullName>
            <ecNumber evidence="10">3.4.26.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100834096</name>
        <name evidence="13" type="ORF">BRADI_2g28087v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="10"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566340.1">
        <property type="nucleotide sequence ID" value="XM_003566292.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HKA2"/>
    <dbReference type="FunCoup" id="I1HKA2">
        <property type="interactions" value="1585"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HKA2"/>
    <dbReference type="EnsemblPlants" id="KQK06731">
        <property type="protein sequence ID" value="KQK06731"/>
        <property type="gene ID" value="BRADI_2g28087v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834096"/>
    <dbReference type="Gramene" id="KQK06731">
        <property type="protein sequence ID" value="KQK06731"/>
        <property type="gene ID" value="BRADI_2g28087v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834096"/>
    <dbReference type="eggNOG" id="KOG4130">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049909_2_0_1"/>
    <dbReference type="OMA" id="VIFLCPI"/>
    <dbReference type="OrthoDB" id="271604at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="12">
        <location>
            <begin position="154"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK06731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="335" mass="36876" checksum="7514F7086401165D" modified="2012-06-13" version="1">MATPAYSQPASPPVIPGTVAVAACFAMAVSYVAVLYAPTVLLRLPPATSLRAFFHRRFVCAAFSSTASVLATASLLRLWSPSNTSKALAVFGIRHDHLLEAVVIPLLLTSLVYAGSFVARLSVMSSWCGRDDGEEGISCTEKLARWMQTSLQDVMVWRNYIVAPFTEELVFRACMIPLLLCGGFKMSYIIFLSPVFFSLAHLNHLFELHQQGCNFVRSLLIVGIQLGYTVIFGWYAAFLFIRTGNLVSPIVAHIFCNMMGLPVFSSTQTRGMASLAFLVGSISFFCVLFPATSPRLYNTRLDGCSCWHGYCRWSGIGTEQHVGNITYFVPVAEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>I1HNG4</accession>
    <name>I1HNG4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100822322</name>
        <name evidence="13" type="ORF">BRADI_2g40760v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566767.1">
        <property type="nucleotide sequence ID" value="XM_003566719.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HNG4"/>
    <dbReference type="FunCoup" id="I1HNG4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HNG4"/>
    <dbReference type="EnsemblPlants" id="KQK08262">
        <property type="protein sequence ID" value="KQK08262"/>
        <property type="gene ID" value="BRADI_2g40760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822322"/>
    <dbReference type="Gramene" id="KQK08262">
        <property type="protein sequence ID" value="KQK08262"/>
        <property type="gene ID" value="BRADI_2g40760v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822322"/>
    <dbReference type="eggNOG" id="KOG0331">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_31_1_1"/>
    <dbReference type="OMA" id="HPSNDHT"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HNG4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="WW" evidence="9">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="158"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="189"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="392"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="536"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="7">
        <location>
            <begin position="158"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="550"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="572"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="599"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="619"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="682"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="712"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="796"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK08262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="828" mass="92659" checksum="1D128EEDEA2EED8C" modified="2012-06-13" version="1">MAGTAAPRYAPDDPTLPKPWRGLVDGTTGYLYYWNPDTNITQYEKPLLPEDQLPPPPPLPPPPPRSRGRRDRSRSRSRDRRDRSRSRSRSRTPPRRDHRDRDRDRRRHDEHNSSKQASSYHQLPPVVPTAAPDDPSAEAYRRRHEITVIGDNVPAPITAFDSGVIPSDILKEIQRAGFPSPTPIQAQSWPIALQNQDVVAIAKTGSGKTLGYLLPGFMHIKRLQNSTRSGPTVLVLAPTRELATQILEEAVKFGRSSRISSTCLYGGAPKGPQLRDLERGVDVVVATPGRLNDILEMRKISLKQVSYLVLDEADRMLDMGFEPQIRKIVKDIPSSRQTLMYTATWPKEVRRIADELLVHPVQVTIGSVDELVANKAITQHVEVITPSEKLRRLEQILRSHDSGSKILIFCTTKRMCDQLSRTLNRHFGAAAIHGDKSQNEREKVLSQFRSGRSPILVATDVAARGLDIKDIRVVINYDFPTGVEDYVHRIGRTGRAGATGLAYTFLCDQDAKYAADLIKILEGADQDVPRELMDMVSRGGRGRKRNKWATRSERGGGSRSELDSSRYSGRLESSRSGRGKDDYSGRGRHDYGGSDGRSRRSGRGRSRSRSRSDSDRRSPSPKRRRHDGLKSRSRTRSRSRSRSRSRSRSYTRNRRASRSRSRSPAARHRTERRDGSATARPDSGHRVEHKTSPRVHPSNDHTKHSGSPKVHPSNDHTNHSDQKDDYHLEDGKMGKVDLDRSPSPQDDKSAPYSPVYSKGKDSMSPIGQPVVEAKAVEVSQKPDPASPPHRSKNRDDEEEGIIDEEGEEGMIADDDPRASAAVQNGGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPB5</accession>
    <name>I1HPB5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein pelota homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100821812</name>
        <name evidence="11" type="ORF">BRADI_2g43260v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, pelo recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following ribosome-binding, the Pelota-HBS1L complex promotes the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569340.1">
        <property type="nucleotide sequence ID" value="XM_003569292.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPB5"/>
    <dbReference type="SMR" id="I1HPB5"/>
    <dbReference type="STRING" id="15368.I1HPB5"/>
    <dbReference type="EnsemblPlants" id="KQK08685">
        <property type="protein sequence ID" value="KQK08685"/>
        <property type="gene ID" value="BRADI_2g43260v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821812"/>
    <dbReference type="Gramene" id="KQK08685">
        <property type="protein sequence ID" value="KQK08685"/>
        <property type="gene ID" value="BRADI_2g43260v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821812"/>
    <dbReference type="eggNOG" id="KOG2869">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023334_3_1_1"/>
    <dbReference type="OMA" id="KEQFHGY"/>
    <dbReference type="OrthoDB" id="10249111at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000002">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000002">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF8">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK08685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="377" mass="41951" checksum="6E23081F76256A2C" modified="2012-06-13" version="1">MKLVQSNFARDAPGYAKLLPKVDDDLWDAYNLILAGDSVEAVTFRKITRSGGRDAERVKLTLEIAVDSVEYDKDGSVLRVRGRNISKNEHIQVGQFHTLELELKRPFVLRKELWDWPALDTIRQSCDDTGANADLAVLLMQEGLAQLFLVGRSVAANRARVEAPIPRKHGSAAVSAYDAALKGFFERVLEAFLSHVDFQVVRCVVVASPGFTKEQFHGYMLLEAERRGLRAVLQNKARIVLARAPSGYAHSLQEVLACPGVKALVKDTRLAQEAPALEEFFAMVIKDSDRACYGPKHVDVAHERLAIQTLLFTDTMFRNREVAARRKCVDLAEAVKKQGGTVRVFSSMHVSGNQLEQLTGIAAILRFPLPELDDIEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQD2</accession>
    <name>I1HQD2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Small ribosomal subunit protein bS20c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">30S ribosomal protein S20, chloroplastic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100837078</name>
        <name evidence="13" type="ORF">BRADI_2g46520v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bS20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569559.1">
        <property type="nucleotide sequence ID" value="XM_003569511.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQD2"/>
    <dbReference type="FunCoup" id="I1HQD2">
        <property type="interactions" value="1243"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HQD2"/>
    <dbReference type="EnsemblPlants" id="KQK09181">
        <property type="protein sequence ID" value="KQK09181"/>
        <property type="gene ID" value="BRADI_2g46520v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837078"/>
    <dbReference type="Gramene" id="KQK09181">
        <property type="protein sequence ID" value="KQK09181"/>
        <property type="gene ID" value="BRADI_2g46520v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837078"/>
    <dbReference type="eggNOG" id="ENOG502RXSZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109118_0_0_1"/>
    <dbReference type="OMA" id="REVAYPP"/>
    <dbReference type="OrthoDB" id="4825at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.110:FF:000003">
        <property type="entry name" value="30S ribosomal protein S20, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.110">
        <property type="entry name" value="Ribosomal protein S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00500">
        <property type="entry name" value="Ribosomal_bS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002583">
        <property type="entry name" value="Ribosomal_bS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036510">
        <property type="entry name" value="Ribosomal_bS20_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00029">
        <property type="entry name" value="S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01649">
        <property type="entry name" value="Ribosomal_S20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46992">
        <property type="entry name" value="Ribosomal protein S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK09181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="186" mass="20675" checksum="1E024220B5A968E3" modified="2012-06-13" version="1">MATATSSLFSLSSLSASLPSPSRAPASLSLRAISPQARLSTSYAAFPIGGFGAWASTGRRRGLEVVCAAEPPKTGRQPDSVKKRERQNDRHRIRNHARKAEMRTRMKKVFRALEKLRKKADAQPEEIIEIEKMISEAYKAIDKTVKVGAMHRNTGDHRKSRLARRKKAIEILRGWYVPNAEPTAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HRZ5</accession>
    <name>I1HRZ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RMA</fullName>
            <ecNumber evidence="11">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein RING membrane-anchor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase RMA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100835412</name>
        <name evidence="14" type="ORF">BRADI_2g50940v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">E3 ubiquitin-protein ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The RING-type zinc finger domain is responsible for E3 ligase activity.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="11"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753867.2">
        <property type="nucleotide sequence ID" value="XM_014898381.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314041.1">
        <property type="nucleotide sequence ID" value="XM_024458273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HRZ5"/>
    <dbReference type="FunCoup" id="I1HRZ5">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HRZ5"/>
    <dbReference type="EnsemblPlants" id="KQK09919">
        <property type="protein sequence ID" value="KQK09919"/>
        <property type="gene ID" value="BRADI_2g50940v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09920">
        <property type="protein sequence ID" value="KQK09920"/>
        <property type="gene ID" value="BRADI_2g50940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835412"/>
    <dbReference type="Gramene" id="KQK09919">
        <property type="protein sequence ID" value="KQK09919"/>
        <property type="gene ID" value="BRADI_2g50940v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09920">
        <property type="protein sequence ID" value="KQK09920"/>
        <property type="gene ID" value="BRADI_2g50940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835412"/>
    <dbReference type="eggNOG" id="KOG0823">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055198_0_0_1"/>
    <dbReference type="OMA" id="HPNQSPW"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HRZ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044390">
        <property type="term" value="F:ubiquitin-like protein conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16745">
        <property type="entry name" value="RING-HC_AtRMA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045103">
        <property type="entry name" value="RNF5/RNF185-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12313">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="23"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK09920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="220" mass="23948" checksum="44E75B3CD52C9951" modified="2012-06-13" version="1">MANRVDESSASVNGGMDSGSFECNICLELAQDPVVTLCGHLFCWPCLYEWLHVHAHFPECPVWKAGVQEEKLVPLYGRCKASTGSRSRSVAGVQIPGRPTGQRHSTAPQPDHRHDHYPNQNPWFVGGGGTMAGGRWGNYTFSAAIGGLFPLLNFQAHGFPKAYGPAAELPYGYGGHSFHGWHGNGFPRHDQEPQGQQIDVYLKVLVVLLGVLVIASLIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HS57</accession>
    <name>I1HS57_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="7">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100846508</name>
        <name evidence="10" type="ORF">BRADI_2g51467v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569932.1">
        <property type="nucleotide sequence ID" value="XM_003569884.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HS57"/>
    <dbReference type="FunCoup" id="I1HS57">
        <property type="interactions" value="2667"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HS57"/>
    <dbReference type="EnsemblPlants" id="KQK10004">
        <property type="protein sequence ID" value="KQK10004"/>
        <property type="gene ID" value="BRADI_2g51467v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846508"/>
    <dbReference type="Gramene" id="KQK10004">
        <property type="protein sequence ID" value="KQK10004"/>
        <property type="gene ID" value="BRADI_2g51467v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846508"/>
    <dbReference type="eggNOG" id="KOG2429">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003818_5_5_1"/>
    <dbReference type="OMA" id="HNYHRVW"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097466">
        <property type="term" value="P:ubiquitin-dependent glycoprotein ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF6">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094779" description="alpha-1,2-Mannosidase" evidence="9">
        <location>
            <begin position="32"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="501"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="560"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="624"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="511"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="664"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK10004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="674" mass="74714" checksum="D493957ACAF4A72A" modified="2012-06-13" version="1">MRLRRQGSPLLCLSAALLAVALLTGAAVADGVTPSEARRLRDEVKDMFYHAFDGYMQHAFPLDELRPLSCKGEDSLGGYALTLIDSLDTLALLGDKEKFGAGVEWVGKNVRFDINKTVSVFETNIRILGGLLSAHLIASDYATGMRIQSYDDQLLHLAVDLAQRLLPAFDTPTGIPFGSVNLLYGVDDDESKITSTAGGGTLALEFGILSRLTNNTVFEEVTKNAVRGIWSRRSKLNLVGAHINVFTGEWTQKDAGIGTSIDSFYEYLLKAYLLFGDEEYLYIFQEAYKAAMHYLHLDPWYVEVNMNTGVTVWPLFNSLQAFWPGLQVLAGDVDPAIRTHAAFFSVWKKYGFTPEGFNLATSTVQNGQRSYPLRPELIESTYWLFKATRDYKYLDVGRDILASLQYGARCPCGYCHISDVETHKQDDHMESFFLAETVKYLWLLFDLAVGPDNIVENGPYKYIFSTEGHLLPATPEIALVDEHCSYFGAFCNGSTDRGYSKSDSSSKHQSANNTQFDDRQTASSKYSMSNVLFTTRGYIKGVCPGLTHAQKLGISFYDEGDNSVEQNSKSPENPDESHVVESSVHAQSSAVILMSHHVTSQPDEPLKTKLINWHHETIVVTADSDPMRTDATGGLSEGSTEELSEETISISEQTEVTEEDIASQDIQPNEDSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSR2</accession>
    <name>I1HSR2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100831718</name>
        <name evidence="2" type="ORF">BRADI_2g53150v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232483.1">
        <property type="nucleotide sequence ID" value="XM_010234181.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSR2"/>
    <dbReference type="EnsemblPlants" id="KQK10280">
        <property type="protein sequence ID" value="KQK10280"/>
        <property type="gene ID" value="BRADI_2g53150v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK10281">
        <property type="protein sequence ID" value="KQK10281"/>
        <property type="gene ID" value="BRADI_2g53150v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73092">
        <property type="protein sequence ID" value="PNT73092"/>
        <property type="gene ID" value="BRADI_2g53150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831718"/>
    <dbReference type="Gramene" id="KQK10280">
        <property type="protein sequence ID" value="KQK10280"/>
        <property type="gene ID" value="BRADI_2g53150v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10281">
        <property type="protein sequence ID" value="KQK10281"/>
        <property type="gene ID" value="BRADI_2g53150v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73092">
        <property type="protein sequence ID" value="PNT73092"/>
        <property type="gene ID" value="BRADI_2g53150v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831718"/>
    <dbReference type="eggNOG" id="ENOG502QWB0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_740514_0_0_1"/>
    <dbReference type="OMA" id="KDRTHSH"/>
    <dbReference type="OrthoDB" id="2016966at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34952:SF1">
        <property type="entry name" value="OS01G0814400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34952">
        <property type="entry name" value="OS05G0113500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK10280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="348" mass="37647" checksum="AD7E8F51468F6EF5" modified="2012-06-13" version="1">MGGPLVNSSAKGVGRSLCLCRGTCEKAGFEHSSCACSEEEKFEAGIAFAQDDSVVKEIGASLAEVMHVYDDNNDEGTDSSEDYDENEDSPLSLESDSTDDLVDIDTELITSSAFPVGSAPESSIGKSVDGNSSTNGTPLLVSAMKGSRAKRGIVTELSVSWAPDVYDPPVTSDCHTVKVHQRSSRKSHYKYRPPNGGSSRRSSSGDKKDKKHSHHSSSSSSRRDKKPSYRSTKSSSRIDTGDPQHGKVYDSSISSRTGDPQHYEVYGSTISSRIGPNVPQYHKLSPWLPADSTSPEEAMPVPVLKTMEQIKRSSSCCKEPPLSMLSRQFVAAKYKGMFSLWSQNQLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HVA2</accession>
    <name>I1HVA2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100839190</name>
        <name evidence="5" type="ORF">BRADI_2g61000v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565091.1">
        <property type="nucleotide sequence ID" value="XM_003565043.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HVA2"/>
    <dbReference type="STRING" id="15368.I1HVA2"/>
    <dbReference type="EnsemblPlants" id="KQK11584">
        <property type="protein sequence ID" value="KQK11584"/>
        <property type="gene ID" value="BRADI_2g61000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839190"/>
    <dbReference type="Gramene" id="KQK11584">
        <property type="protein sequence ID" value="KQK11584"/>
        <property type="gene ID" value="BRADI_2g61000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839190"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_5_1"/>
    <dbReference type="OMA" id="IANISSX"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17581">
        <property type="entry name" value="REC_typeA_ARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF106">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ORR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="12"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="148"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK11584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="213" mass="22961" checksum="251BE281EE860998" modified="2012-06-13" version="1">MTVVAAAESRFHVLAVDDSVIDRKLIEMLLKTSSYQVTTVDSGSKALEVLGLRDEGDSSSASSSPSSSSPDHQEVDVNLIITDYCMPGMTGYDLLRRVKGSSSLKDIPVVIMSSENVPARINRCLEDGAEEFFLKPVKLADMKKLKSHLLRRKQPQKPEQAPDKPAEEAAAAEVTVSGSSKKRKAAAAMDQEGLGSPERTKPRLSSSSLAVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1HW45</accession>
    <name>I1HW45_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA-binding protein 48</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100835948</name>
        <name evidence="11" type="ORF">BRADI_3g00804v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RBM48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570574.1">
        <property type="nucleotide sequence ID" value="XM_003570526.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HW45"/>
    <dbReference type="STRING" id="15368.I1HW45"/>
    <dbReference type="EnsemblPlants" id="KQJ92801">
        <property type="protein sequence ID" value="KQJ92801"/>
        <property type="gene ID" value="BRADI_3g00804v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835948"/>
    <dbReference type="Gramene" id="KQJ92801">
        <property type="protein sequence ID" value="KQJ92801"/>
        <property type="gene ID" value="BRADI_3g00804v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835948"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103539_0_0_1"/>
    <dbReference type="OMA" id="NSHRQMS"/>
    <dbReference type="OrthoDB" id="78358at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HW45">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12442">
        <property type="entry name" value="RRM_RBM48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000424">
        <property type="entry name" value="RNA-binding protein 48 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039599">
        <property type="entry name" value="RBM48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034264">
        <property type="entry name" value="RBM48_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20957">
        <property type="entry name" value="RNA-BINDING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20957:SF0">
        <property type="entry name" value="RNA-BINDING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="21"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="121"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ92801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="224" mass="25125" checksum="E6D99D8CF8C4C1D5" modified="2012-06-13" version="1">MARDRGEPAAVRVYTVCDESKYLIVRNVPSLGCGDELGTLFSTYGPLEECTPMDGEDCEEYTDVFFIKFSQVSNARFAKRKLDESVFLGNRLQVSYAPQFESVLDTKEKLEVRRKEVLSRIRSPAGSRPEGVSQYSLGQGSSSGNSHHHMSSNKRDYTQAMHASHIKDARFSHVSSNKDYFPSESMNATVNLVREKLDQIQSGSDNSNAVTASKKPKVDNRRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I1HW73</accession>
    <name>I1HW73_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Knottin scorpion toxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100831744</name>
        <name evidence="2" type="ORF">BRADI_3g01010v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571645.1">
        <property type="nucleotide sequence ID" value="XM_003571597.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HW73"/>
    <dbReference type="EnsemblPlants" id="KQJ92836">
        <property type="protein sequence ID" value="KQJ92836"/>
        <property type="gene ID" value="BRADI_3g01010v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831744"/>
    <dbReference type="Gramene" id="KQJ92836">
        <property type="protein sequence ID" value="KQJ92836"/>
        <property type="gene ID" value="BRADI_3g01010v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831744"/>
    <dbReference type="eggNOG" id="ENOG502R3WQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172795_0_0_1"/>
    <dbReference type="OMA" id="CSSCCKN"/>
    <dbReference type="OrthoDB" id="691018at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094829" description="Knottin scorpion toxin-like domain-containing protein" evidence="1">
        <location>
            <begin position="32"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ92836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11608" checksum="02F7B08BD477FEF8" modified="2012-06-13" version="1">MTGKKKQPAPLLVLLAILLITTLEMVPEIAAAGNEKLCDVLSPRCGGMCYVSKCSSCCKNHGLSDGHCRLNHGIVCYCCRGPNSGEQQRMLKVIPASPPPPRRPLLHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HX58</accession>
    <name>I1HX58_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">4-coumarate--CoA ligase</fullName>
            <ecNumber evidence="2">6.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100842259</name>
        <name evidence="9" type="ORF">BRADI_3g03730v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(E)-4-coumarate + ATP + CoA = (E)-4-coumaroyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19641"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:19642"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(E)-4-coumarate + ATP + H(+) = (E)-4-coumaroyl-AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:72419"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:72420"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(E)-4-coumaroyl-AMP + CoA = (E)-4-coumaroyl-CoA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:72423"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:72424"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.2.1.12" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574567.1">
        <property type="nucleotide sequence ID" value="XM_003574519.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233809.1">
        <property type="nucleotide sequence ID" value="XM_010235507.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316624.1">
        <property type="nucleotide sequence ID" value="XM_024460856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HX58"/>
    <dbReference type="STRING" id="15368.I1HX58"/>
    <dbReference type="EnsemblPlants" id="KQJ93302">
        <property type="protein sequence ID" value="KQJ93302"/>
        <property type="gene ID" value="BRADI_3g03730v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93303">
        <property type="protein sequence ID" value="KQJ93303"/>
        <property type="gene ID" value="BRADI_3g03730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842259"/>
    <dbReference type="Gramene" id="KQJ93302">
        <property type="protein sequence ID" value="KQJ93302"/>
        <property type="gene ID" value="BRADI_3g03730v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93303">
        <property type="protein sequence ID" value="KQJ93303"/>
        <property type="gene ID" value="BRADI_3g03730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842259"/>
    <dbReference type="eggNOG" id="KOG1180">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_45_2_1"/>
    <dbReference type="OMA" id="CAELVCI"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016207">
        <property type="term" value="F:4-coumarate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106290">
        <property type="term" value="F:trans-cinnamate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17639">
        <property type="entry name" value="LC_FACS_euk1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF92">
        <property type="entry name" value="LONG CHAIN ACYL-COA SYNTHETASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="125"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ93303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="726" mass="80003" checksum="47D249527F295F74" modified="2012-06-13" version="1">MKKTTGENTNMSILERLATSDVPLVKEYGLHGVIGALLLAMVIPILLSSMLSKKVKKRAVQTDVGGEAGLAMRNSRFSSLIQVPWEGATTMAALFEMASKKYPQHRCLGSRNLISSEFIEAADGRKFEKLHLGEYEWNSYAEAFNRACNFASGLIKMGHQPDSRAAIFSDTRAEWIIASQGCFRQNLTVVTIYASLGEDALVHSLNETQVSTLICDSKQLKKLLSVSSKLQSVRHVIYIEDEPVDADTLNQLRHLTTLSFYAVEELGKTSHTDPRLPSSSDTAVIMYTSGSTGLPKGVMITHGNMVATTAAVRTIIPKLGTGDVYLAYLPLAHVFELAAETVMLASGVAIGYGSALTMTDTSNKIKKGTKGDVSVLKPTLMISVPAILDRIRDAVFKKVAEKGGMKKKLFDVSYKRNLGAIEGSWFGSWAPERLIWDSIVFKPIRLMLGGRIRFILCGGAPLSSDTQRFINICLGVPVGQGYGLTETCAGAAFSEWDDTSVGRVGPPLPCCYVKLISWEEGGYRISDSPMPRGEVVIGGYSITKGYFNNEEKTNEVYKVDERGIRWFYTGDIGQFHPDGCVEIIDRKKDIVKLQHGEYVSLGKVESALQTSNYVDNIMVYADPFHSYCVALVVPPHQALEKWAQNSGINYKNFEELCQNGQALKEVQQSLSKAAKAARLEKFEMPAKIILLAEPWTPESGLVTAALKLKREQLKTKFKDDLNKLYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXZ3</accession>
    <name>I1HXZ3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100836462</name>
        <name evidence="11" type="ORF">BRADI_3g06070v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WRKY group II-a family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570741.1">
        <property type="nucleotide sequence ID" value="XM_003570693.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXZ3"/>
    <dbReference type="FunCoup" id="I1HXZ3">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HXZ3"/>
    <dbReference type="EnsemblPlants" id="KQJ93670">
        <property type="protein sequence ID" value="KQJ93670"/>
        <property type="gene ID" value="BRADI_3g06070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836462"/>
    <dbReference type="Gramene" id="KQJ93670">
        <property type="protein sequence ID" value="KQJ93670"/>
        <property type="gene ID" value="BRADI_3g06070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836462"/>
    <dbReference type="eggNOG" id="ENOG502QR7M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047067_0_0_1"/>
    <dbReference type="OMA" id="DPWIGSQ"/>
    <dbReference type="OrthoDB" id="1879341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009938">
        <property type="term" value="P:negative regulation of gibberellic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010200">
        <property type="term" value="P:response to chitin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009723">
        <property type="term" value="P:response to ethylene"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009739">
        <property type="term" value="P:response to gibberellin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009753">
        <property type="term" value="P:response to jasmonic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002237">
        <property type="term" value="P:response to molecule of bacterial origin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002238">
        <property type="term" value="P:response to molecule of fungal origin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009751">
        <property type="term" value="P:response to salicylic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000008">
        <property type="entry name" value="WRKY transcription factor 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429:SF114">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR WRKY71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="10">
        <location>
            <begin position="180"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="92"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="239"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="263"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ93670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="345" mass="37683" checksum="176D33D35C0C2BB2" modified="2012-06-13" version="1">MDPWIGSQPSLSLDLHVGLGHHHRHQAQPVVALVKPKILVEENFLPLKKDPEVAGLEAELQRVSEENRRLGEMLREVAAKYESLQGQYSDMTNAANNYSHPSSASTEGGSVSPSRKRKSSDSLDGHTPSPPPPPQLSFVPEQQAECTSGEPCNKRVRPAAASEECKPVVSKRYVHADPSDLSLVVKDGYQWRKYGQKVTKDNPCPRAYFRCSFAPACPVKKKVQRSAEDRTVLVATYEGEHNHGQPPPAQPQQQQQHDGKNGAAKPPQAPVAAAAALHQQHQQQKQEQEAAVVNGEAAAAASELIRRNLAEQMAMTLTMDPSFKAALVTALSGRIFELSPTKDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1I0J0</accession>
    <name>I1I0J0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="18 19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100823380</name>
        <name evidence="18" type="ORF">BRADI_3g13840v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function as sodium-coupled metabolite transporter across the chloroplast envelope.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571324.1">
        <property type="nucleotide sequence ID" value="XM_003571276.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I0J0"/>
    <dbReference type="STRING" id="15368.I1I0J0"/>
    <dbReference type="EnsemblPlants" id="KQJ94885">
        <property type="protein sequence ID" value="KQJ94885"/>
        <property type="gene ID" value="BRADI_3g13840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823380"/>
    <dbReference type="Gramene" id="KQJ94885">
        <property type="protein sequence ID" value="KQJ94885"/>
        <property type="gene ID" value="BRADI_3g13840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823380"/>
    <dbReference type="eggNOG" id="KOG1650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_6_2_1"/>
    <dbReference type="OMA" id="DRNCVPS"/>
    <dbReference type="OrthoDB" id="1889525at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000028">
        <property type="entry name" value="Cation/H(+) antiporter 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057291">
        <property type="entry name" value="CHX17_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057290">
        <property type="entry name" value="CHX17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050794">
        <property type="entry name" value="CPA2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468">
        <property type="entry name" value="CATION/H + ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468:SF102">
        <property type="entry name" value="OS08G0117800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23256">
        <property type="entry name" value="CHX17_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23259">
        <property type="entry name" value="CHX17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="15">
        <location>
            <begin position="50"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter central" evidence="16">
        <location>
            <begin position="541"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter C-terminal" evidence="17">
        <location>
            <begin position="664"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="811"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="811"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="824"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQJ94885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="836" mass="89365" checksum="404F8065C24B1FD9" modified="2012-06-13" version="1">MSLGEATVNKSLFCFPVDSGATSSGLFAGDDPLKFYFPLLLYHVCIVFILSHSIHSFLLRRSVPLVISQIIAGALLGPSVLGHLAPSVGKLFASPEGWVQINTVGGYAFTLHIFTVGVKTDLGMIVKSGKKAVAIAFLGTAAPHLAMYLAGAALSSRIPKQWSDTFLMTNLNSWWSLSAFIVVCCTLHDLNLLSSKLGRLAMSAALIGDFANTFSIAGVTSYLLASSPEEKLQRIGFASSMSFSVFIALMALVARPAILRLIQDVPDGALLSEGRLVAVLLISLTCSFAGELLGLHATYGPFMLGLMLPGGAPLGVTLEERLDRLIVGILSPLLFAQGGMRMNVHALTDSSVCGLLEIFLVVGVAAKFVASIIPCLYCGIPPREAAFVGLMMNFKGITEVVYASAFMDAKILDDQVYVAFMINVLVIGASTAAAVKFMYHPEEKYVAYRRRTVQHKKLGEELRVLACIHSQADVEPMLGLLDASSPTPLSPLSVYLLHLVPLAGLTSSVLRPFKHGHGHGGDGEDGEGRTIGVIPSASERIVNAFRFFAQNRPMGSSSLLPYVCIAPYATMHDDVCAVALEKRAMLIVVPFHKRLAIDGSVEPTSPGCSGAIQAANANILGYSPCSVAILFDRGSLSCGAAASPAATAGAAGGEEEFQRFPYRVALYFLGGPDDREALAFAAHMAEDAPIGVTVFRFLLPVEWQRRLDGAEEDRLDEEATREFVGQWVDDHRVVYNEHTVGTSDEMVAVIRQTSPGFNLLLVGRRAESRESPLTAGISDWSEHSELGVLGDLLTSADFGCRVSTLVVQQQTRAAAGETSQTPPEDQPPLHLPDGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5X8</accession>
    <name>I1I5X8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carotene epsilon-monooxygenase, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100835344</name>
        <name evidence="7" type="ORF">BRADI_3g32690v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574294.1">
        <property type="nucleotide sequence ID" value="XM_003574246.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5X8"/>
    <dbReference type="STRING" id="15368.I1I5X8"/>
    <dbReference type="EnsemblPlants" id="KQJ97695">
        <property type="protein sequence ID" value="KQJ97695"/>
        <property type="gene ID" value="BRADI_3g32690v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ97696">
        <property type="protein sequence ID" value="KQJ97696"/>
        <property type="gene ID" value="BRADI_3g32690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835344"/>
    <dbReference type="Gramene" id="KQJ97695">
        <property type="protein sequence ID" value="KQJ97695"/>
        <property type="gene ID" value="BRADI_3g32690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97696">
        <property type="protein sequence ID" value="KQJ97696"/>
        <property type="gene ID" value="BRADI_3g32690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835344"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_5_1"/>
    <dbReference type="OMA" id="WPHPETF"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072374">
        <property type="term" value="F:carotene epsilon hydroxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11046">
        <property type="entry name" value="CYP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF134">
        <property type="entry name" value="CAROTENE EPSILON-MONOOXYGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="4">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ97695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="61416" checksum="7C093C76365BBD7F" modified="2012-06-13" version="1">MPAAALAFASTFAPPQPWVPRRPHPRHARLRLPPPRSGGGDNNKPTTSWVSPDWLTSLSRSLVGRGGGGGDGSGIPVASAKLDDVRDLLGGALFLPLFKWFREEGPVYRLAAGPRDFVIVSDPAVAKHVLRGYGTRYEKGLVAEVSEFLFGSGFAIAEGALWTVRRRAVVPSLHKRFISVMVEKVFCKCAERLVEKLETYALSSEPVNMEARFSQMTLDVIGLSLFNYNFDSLTSDSPVIDAVYTALKEAEARSTDLLPYWQIDLLRKIVPRQIKAEKAVNIIRNTVEELITKCKTIVDAENEQIEGDEYVNEADPSILRFLLASREEVNSSQLRDDLLSMLVAGHETTGSVLTWTVYLLSKDPAALRRAQDEVDRVLQGRLPRYEDVKELKYLMRCINESMRLYPHPPVLIRRAIVDDVLPGNYKVKAGQDIMISVYNIHRSPEVWDRADEFVPERFDLEGPVPNESNTDFRFVPFSGGPRKCVGDQFALLEAIVALAVVLQKIDIQLVADQEINMTTGATIHTTNGLYMNVSLRRVEQEPELALSDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I1I7A0</accession>
    <name>I1I7A0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100834325</name>
        <name evidence="2" type="ORF">BRADI_3g36550v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572222.1">
        <property type="nucleotide sequence ID" value="XM_003572174.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I7A0"/>
    <dbReference type="FunCoup" id="I1I7A0">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ98379">
        <property type="protein sequence ID" value="KQJ98379"/>
        <property type="gene ID" value="BRADI_3g36550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834325"/>
    <dbReference type="Gramene" id="KQJ98379">
        <property type="protein sequence ID" value="KQJ98379"/>
        <property type="gene ID" value="BRADI_3g36550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834325"/>
    <dbReference type="eggNOG" id="ENOG502R7PA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098433_0_0_1"/>
    <dbReference type="OMA" id="AVFRTTC"/>
    <dbReference type="OrthoDB" id="693567at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ98379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="282" mass="30463" checksum="6D3641696D1654F4" modified="2012-06-13" version="1">MGRLQEHFSSNAGDTSPFLGPSPPLFGCDSSSPSPLLLPAPPGGKRGFGGRRSSRHVIVKLICSPFAAVFRTTCSPRAHSIGDDDDLTSGAGDEQEGQAARRRPRLEELLKMESASSDPEELIKKPTELVPADMDDSWKESAIVVFEAFGDEKDDTSRTTGEEEEEEEEEDDQLEYALPPGRQMAIVRPAGLGVSQTAVGGGAALMNVKRLVLLLEAMRARSMALKVKAGSSSSYWRLSGPGPGPGGRAADKKADQLLFYDRPIPLGRRCRVQHLQETSPYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1I7X4</accession>
    <name>I1I7X4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase subunit d, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100840446</name>
        <name evidence="12" type="ORF">BRADI_3g38390v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATPase d subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235222.1">
        <property type="nucleotide sequence ID" value="XM_010236920.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I7X4"/>
    <dbReference type="FunCoup" id="I1I7X4">
        <property type="interactions" value="1470"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I7X4"/>
    <dbReference type="EnsemblPlants" id="KQJ98678">
        <property type="protein sequence ID" value="KQJ98678"/>
        <property type="gene ID" value="BRADI_3g38390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840446"/>
    <dbReference type="Gramene" id="KQJ98678">
        <property type="protein sequence ID" value="KQJ98678"/>
        <property type="gene ID" value="BRADI_3g38390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840446"/>
    <dbReference type="eggNOG" id="KOG3366">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089809_1_0_1"/>
    <dbReference type="OMA" id="TPQYKAK"/>
    <dbReference type="OrthoDB" id="35799at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008689">
        <property type="entry name" value="ATP_synth_F0_dsu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036228">
        <property type="entry name" value="ATP_synth_F0_dsu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12700">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT D, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05873">
        <property type="entry name" value="Mt_ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005514">
        <property type="entry name" value="ATPase_F0_D_mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161065">
        <property type="entry name" value="ATP synthase D chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="110"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ98678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="168" mass="19590" checksum="8E1D16F8A99B7146" modified="2012-06-13" version="1">MSGSGKKVADLAVKAGKAIDWDGMAKMLVSEEARKEFATLRRTFEDVNNQLQTKFSQEPKPIDWEYYRKGIGAKVVDMYKEAYDSIEIPKYVDTVTPEYKPKFDALLVELKEAEQMSLKESERIEKEIAELREMKKKISTMTADEYFAKHPELKQKFDDEIRNDNWGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1I985</accession>
    <name>I1I985_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100837099</name>
        <name evidence="3" type="ORF">BRADI_3g42170v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574886.1">
        <property type="nucleotide sequence ID" value="XM_003574838.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I985"/>
    <dbReference type="STRING" id="15368.I1I985"/>
    <dbReference type="EnsemblPlants" id="KQJ99269">
        <property type="protein sequence ID" value="KQJ99269"/>
        <property type="gene ID" value="BRADI_3g42170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837099"/>
    <dbReference type="Gramene" id="KQJ99269">
        <property type="protein sequence ID" value="KQJ99269"/>
        <property type="gene ID" value="BRADI_3g42170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837099"/>
    <dbReference type="eggNOG" id="KOG2197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004457_5_0_1"/>
    <dbReference type="OMA" id="RLEVWPF"/>
    <dbReference type="OrthoDB" id="10264062at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000014">
        <property type="entry name" value="GTPase-activating protein gyp7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000022">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF507">
        <property type="entry name" value="OS08G0547200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="123"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ99269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="562" mass="63523" checksum="8ECF4E40BBC446F0" modified="2012-06-13" version="1">MKALRRSSTSSSSSSSSSPRTPSSPPPPSSWRHRRSLLLVASSSLAAAAKSAAPAPAMLAADSSASSQALYSPNSDRGGTKSPWSRRKRKGALSCERWRQLFSSNGRLRDGGRKFLKKVRSGGIEPGIRAEVWPFLLGVYDLNSSEEERNTIKIKKRNEYEKLRRKCHQILNCYKGFELKVINEVTNEGCSGGATYMEDVSTSVSVSPKEFSCLVSKANSPESADRAAAECMDEDISELIHVDQCMAESESSESESSDEDDHGQISMGANIEENCAPEPKFVRSASSKSDFFRSNKTPEDFATWQRIIRLDAIRANTDWALFSHNQAEVCKENALQHALSIGLKDYDHLEPYMIYHAARLVALLEAYALYDPEIGYCQGMSDLLSPIIAVMEEDHEAFWCFVGFMRKARHNFRLDEVGIKTQLKTVSRIIKRKDSHLYRHLQKLQAEDCFFVYRMVLVLFRRELTFEQTLCLWEVMWADQAAIRAGIRRSTWGKIRLHAPPTDDLLLYAIAACVLQRRKLIIERYSSMDEILRECQSMAGQLDVWRLLDDAHDLVVNLHNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDS8</accession>
    <name>I1IDS8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-ketoacyl-CoA synthase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100824293</name>
        <name evidence="9" type="ORF">BRADI_3g55040v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570371.1">
        <property type="nucleotide sequence ID" value="XM_003570323.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDS8"/>
    <dbReference type="STRING" id="15368.I1IDS8"/>
    <dbReference type="EnsemblPlants" id="KQK01305">
        <property type="protein sequence ID" value="KQK01305"/>
        <property type="gene ID" value="BRADI_3g55040v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69424">
        <property type="protein sequence ID" value="PNT69424"/>
        <property type="gene ID" value="BRADI_3g55040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824293"/>
    <dbReference type="Gramene" id="KQK01305">
        <property type="protein sequence ID" value="KQK01305"/>
        <property type="gene ID" value="BRADI_3g55040v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69424">
        <property type="protein sequence ID" value="PNT69424"/>
        <property type="gene ID" value="BRADI_3g55040v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824293"/>
    <dbReference type="eggNOG" id="ENOG502QPV6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013238_3_1_1"/>
    <dbReference type="OMA" id="WEDCIAD"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095287" description="3-ketoacyl-CoA synthase" evidence="6">
        <location>
            <begin position="26"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="FAE" evidence="7">
        <location>
            <begin position="25"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="8">
        <location>
            <begin position="337"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036417-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK01305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="465" mass="51086" checksum="0EB20B811F923628" modified="2012-06-13" version="1">MELLPLLSWLLLAFASSYLAHLAVSRRRRSRCYLLGYACHKPSDDRKVTTEMAGAVIERNKRLGLPDYRFLLKVIVNSGIGEHTYSPRNVLDGREESATHLDALDEMDAFFDDAVARVFATTGVSPRQVDLLVLNVGSFSPAPSLASRVASRFGMRDDVMAYNLSGMGCSAGLVSVDLARRVMLTRPGATMALVVTSESCAPNWYNGTDKSMMLGNCLFRCGGAAALLTNDPALRGRAKMELKCLVRAHIGAHDDAHAAAVHREDADGRLGVSLSKNLPKAAVRAFTENLQRLAPRILPASELARFTFRLLARKLLRHGGRKVVKFEGPKIDFKTGVDHFCLHPGGTAVIDAVRKNLGLNGYDVEPATMTLHRWGNTSASSLWYVLSYMEAKRRLKAGDRVLMVTFGSGFKCNSCYWEVTKDLDDAGVWEDCIDDYPPATMVNPYTEKFGWVNDLQGQGGAGFPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEH8</accession>
    <name>I1IEH8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100841134</name>
        <name evidence="15" type="ORF">BRADI_3g56930v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May play a role in lysosome motility. May play a role in chromosome segregation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with tubulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEH8"/>
    <dbReference type="FunCoup" id="I1IEH8">
        <property type="interactions" value="2333"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IEH8"/>
    <dbReference type="EnsemblPlants" id="KQK01594">
        <property type="protein sequence ID" value="KQK01594"/>
        <property type="gene ID" value="BRADI_3g56930v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01594">
        <property type="protein sequence ID" value="KQK01594"/>
        <property type="gene ID" value="BRADI_3g56930v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="RFRSEWG"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IEH8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:chromosome segregation"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:defense response to virus"/>
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        <property type="project" value="EnsemblPlants"/>
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        <property type="term" value="P:protein transport"/>
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    <dbReference type="CDD" id="cd04159">
        <property type="entry name" value="Arl10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADP-ribosylation factor-like protein 8a"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="Arl8a/8b"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
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    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
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    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
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        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK01594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="188" mass="21099" checksum="57099D8FF0D9F69A" modified="2012-06-13" version="1">MFKLPLLRTITRSRIFSLFFKQEMELSLVGLQNAGKTSLVNSIATGGYSEDMIPTVGFNMRKVTKGNVTIKLWDLGGQRRFRTMWERYCRGVSAILYVVDAADRDSVPIAKSELHDLLTKQSLAGIPLLILGNKIDKSEALSKQALVDQLGLESIKDREVCCYMISCKDTVNIDVVIDWLIKHSKTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>I1IFG9</accession>
    <name>I1IFG9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dynamin GTPase</fullName>
            <ecNumber evidence="1">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100830643</name>
        <name evidence="12" type="ORF">BRADI_3g59800v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="1"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK02008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573124.1">
        <property type="nucleotide sequence ID" value="XM_003573076.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IFG9"/>
    <dbReference type="STRING" id="15368.I1IFG9"/>
    <dbReference type="EnsemblPlants" id="KQK02008">
        <property type="protein sequence ID" value="KQK02008"/>
        <property type="gene ID" value="BRADI_3g59800v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830643"/>
    <dbReference type="Gramene" id="KQK02008">
        <property type="protein sequence ID" value="KQK02008"/>
        <property type="gene ID" value="BRADI_3g59800v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830643"/>
    <dbReference type="HOGENOM" id="CLU_016157_1_0_1"/>
    <dbReference type="OrthoDB" id="5562561at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IFG9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000017">
        <property type="entry name" value="Dynamin-2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000212">
        <property type="entry name" value="Dynamin-2A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dynamin-2B isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF57">
        <property type="entry name" value="DYNAMIN-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="11">
        <location>
            <begin position="41"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="581"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="10">
        <location>
            <begin position="743"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="513"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="639"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="705"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="806"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="533"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="556"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="642"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="816"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="839"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="876"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="912"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK02008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="921" mass="100066" checksum="78211BC757AE3FE2" modified="2012-06-13" version="1">MSSSAAMEAIDELVQLSESMRQAASLLADDDPSDDAAPRRPSTFLNAVALGNVGSGKSAVLNSLIGHPVLPTGENGATRAPIVVDLQRDPGLSTKSIVIQIDSKSQQVSASALRHSLQDRLGKAVSSGRSRTDEIYLKLRTSTAPPLKLIDLPGIDQRVIDDSTINEFAGHNDAILIVVIPAMQAAEVASSRALRLAKDIDPDGTRTIGVLSKIDQAAADAKTVACVQAILSNKGPRAATEIEWVALIGQSVALASAQSGSVGSENSLETAWRAEAETLKSILTGAPQSKLGRIALVDTIAKQIRNRMKVRLPSLLSGLQGKSQIVKDELARLGEQKVESTEGTRAVALELCREFEDKFLAHVTSGEGSGWKIVASFEGKFPDRIKQLPLDRHFDLSNVKRVVLEADGYQPYLISPEKGLKSLIKGVLEMAKEPSRLCVEEVHRVLLDIVNATANGTPGLGRYPPFKREVITIASNALDTFKNDAKKMVVALVDMERAFVPPQHFIRLVQRRMERQRREDEVKTRSSKKAQDAEQSMMNKGSSAQTGSEAGGSLKSSKDKSSQQDKDSKEGSNLQVAGPGGEITAGYLLKKSAKNNEWSKRWFVLNEKSGKLGYTKKQEERHFRGVIVLEECNLEEIEEEELSKSSKDSKKANGSEKGPSLVFKITNRVAYKTVLKAHSAVILKAENMADKIEWMKKIRGIIQSKGGSVKGPNAPEGGSMRQSHSDGSLDTMARRPADPEEELRWMSQEVRGYVEAVLNSLAANVPKAIVLCQVEKAKEDMLNQLYSSISSQSNAKIEELLQEDHNAKRRREKAQKQSSLLSKLTRQLSIHDNRAAVASYSSDNSGTESSPQSPSHAGEDWRSAFDSAANGSADRSSSHNESRSRSADSRSRRYENGDANGANSGSRRTPNRLPPAPPGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IHQ7</accession>
    <name>I1IHQ7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g05350v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IHQ7"/>
    <dbReference type="FunCoup" id="I1IHQ7">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IHQ7"/>
    <dbReference type="EnsemblPlants" id="KQJ86415">
        <property type="protein sequence ID" value="KQJ86415"/>
        <property type="gene ID" value="BRADI_4g05350v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ86415">
        <property type="protein sequence ID" value="KQJ86415"/>
        <property type="gene ID" value="BRADI_4g05350v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S851">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_28_8_1"/>
    <dbReference type="InParanoid" id="I1IHQ7"/>
    <dbReference type="OMA" id="KAMNGRM"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001138">
        <property type="entry name" value="RNA-binding (RRM/RBD/RNP motifs) family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="26"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ86415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="108" mass="11962" checksum="7B800F2CABE4241F" modified="2012-06-13" version="1">MMMRTMRGAGLLGLASRVAGTKGFSTEIFVSKLSFYTTEEELKNIFSPFGAIEEARLMRDNQTGRPKGFGFVKYSSQVAAEKAVKAMDGRIIRGRIIFVETAKERDTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1IIS4</accession>
    <name>I1IIS4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100828216</name>
        <name evidence="8" type="ORF">BRADI_4g08380v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP (TC 2.A.55) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575571.2">
        <property type="nucleotide sequence ID" value="XM_003575523.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IIS4"/>
    <dbReference type="FunCoup" id="I1IIS4">
        <property type="interactions" value="1730"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IIS4"/>
    <dbReference type="EnsemblPlants" id="KQJ86905">
        <property type="protein sequence ID" value="KQJ86905"/>
        <property type="gene ID" value="BRADI_4g08380v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828216"/>
    <dbReference type="Gramene" id="KQJ86905">
        <property type="protein sequence ID" value="KQJ86905"/>
        <property type="gene ID" value="BRADI_4g08380v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828216"/>
    <dbReference type="eggNOG" id="KOG1291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006509_0_0_1"/>
    <dbReference type="OMA" id="DEYDTWT"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006828">
        <property type="term" value="P:manganese ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017187">
        <property type="entry name" value="EIN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF93">
        <property type="entry name" value="NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037378">
        <property type="entry name" value="EIN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="493"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="619"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="836"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="493"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="512"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="542"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="619"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ86905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1264" mass="137963" checksum="2A542E3B896FC3BF" modified="2012-06-13" version="1">MDDGVRSMAECVGAGGGGGRNNLFRTLGPALFISMGYIDLGKWVTSVDAGSRFGYDLVLLVLLFNFSAVLCQYLSICIGMVTEKNLAQICCQEYSQPTCAGLGIQALLSLLTAEITMISGIALGFNLVFEYDDVLTGIWFASVAVNLLPYALSHLDKKMAGTLNACIAGLALVCFVLGLLVSQPKVPLDMNVMFPKLSGESAYSLMALLGGNVIVHNFYVHSSFVQAQKRSPVTLGALFHDHLVSILFIFCGVFLVNYVLMSSAAVGPGNTLLLTFQDVVELMSQIFMNPAAPLLFLVILLLSSHIISLSSIIGSHAIADNFFGITLPLSAHHLLLKVFAMIPTIYYAKIVGSEAIYQLLVICPVIQAMILPSSVIPVFRVSSSRSIMGSYRISSSVEILAFLAFLLMLFTNVIFVAEILFGDSTWTNNMKGNTGSPVVLPYTVIVLISCASLAFTLFLAVTPLKSASNEAETLELFVHSQREPLGTTHHIEEASREDIAHEEVQRPSIDTVLRDPVEIHQKSALEHTESSDTTVESDHDTQQSTDYKLNTPKAQPSLPVYHEEPKPVCVADWAESVPKVSTATAVEHINAENIKAKSTTEKDVEVVPEVCTERDNVASHNLEHEKSAACRAPVSPDGPPSLTFSRAKDSEAGNGSGSLSTLSGLGRAARRQLAATLDEFWGHLFDYHGKLTQDANDKRYSFLLGLDLRTASSAVRIDNQTIEALKSPLMRDAVRGSATSLNSWDSMSRDKELRNLDWNSGHQMGAMGSSNWSQSMNLPYTDLSSPSSSLLEQNAKYYSNFNVPSYSDNQFYQPATIHGYQLASYLKGINASRSQHSNIPLDPRRVPRSSESSFPNYADSAMHARSQTVRGSLGANSLQSPTMNRLNAMAERPYYDSTSIDESESVGSPAYSKKYHSSPDISAMIAASRKALLNEANLGGIAGNQSYLSKLASERSQYMDSAARSKAQIEFNERSQHNLQRDVLSMQLSMNPNTKSLWAQQPFEQLFGVSSAELSKSEMNTGQRSSGITKDDSSYAECEAELLQSLRLCIMKISKVEGSGWLFRQNGGCDESLIDQVAAAERFSQETTENLLSADLRRMPSDKSSQTLRRNDERATNCMRGLPNCGENCVWQAPLVVSFGVWCIRRVLDLSLVESRPELWGKYTYVLNRLQGILEPAFSKPRKPPTGCTCLQTAGPISRPISCSFTTATVILETIKDVEQAISGRKGRSGTAAGDVAFPKGKENLASVLKRYKRRLSSKPSAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQD6</accession>
    <name>I1IQD6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100841069</name>
        <name evidence="3" type="ORF">BRADI_4g31100v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239379.1">
        <property type="nucleotide sequence ID" value="XM_010241077.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQD6"/>
    <dbReference type="STRING" id="15368.I1IQD6"/>
    <dbReference type="EnsemblPlants" id="KQJ90367">
        <property type="protein sequence ID" value="KQJ90367"/>
        <property type="gene ID" value="BRADI_4g31100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841069"/>
    <dbReference type="Gramene" id="KQJ90367">
        <property type="protein sequence ID" value="KQJ90367"/>
        <property type="gene ID" value="BRADI_4g31100v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841069"/>
    <dbReference type="eggNOG" id="ENOG502S0KJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098106_2_0_1"/>
    <dbReference type="OMA" id="GMKQITR"/>
    <dbReference type="OrthoDB" id="1916968at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF7">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ90367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="172" mass="18901" checksum="4BBB0FA65577DF79" modified="2012-06-13" version="1">MGDEGAKAAAATTIVRLRELLHKWALGASDDGGEKLKAHHAAEEEEEAPASIPPFVLRRLRRTATVDSDDESCHSPEAAPDVPRGYCPVYVGMEQRRFVIPTSYLGHPVFRLLLEKAEEEFGFRQEGALAIPCETEAFKYILQCVERHDKGLAAADVDDENLAALQEKASSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IT58</accession>
    <name>I1IT58_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AT-hook motif nuclear-localized protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100829943</name>
        <name evidence="5" type="ORF">BRADI_4g38870v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576887.1">
        <property type="nucleotide sequence ID" value="XM_003576839.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IT58"/>
    <dbReference type="STRING" id="15368.I1IT58"/>
    <dbReference type="EnsemblPlants" id="KQJ91641">
        <property type="protein sequence ID" value="KQJ91641"/>
        <property type="gene ID" value="BRADI_4g38870v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829943"/>
    <dbReference type="Gramene" id="KQJ91641">
        <property type="protein sequence ID" value="KQJ91641"/>
        <property type="gene ID" value="BRADI_4g38870v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829943"/>
    <dbReference type="eggNOG" id="ENOG502QRFG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039808_2_0_1"/>
    <dbReference type="OMA" id="EWTAHAR"/>
    <dbReference type="OrthoDB" id="1932529at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.80:FF:000001">
        <property type="entry name" value="AT-hook motif nuclear-localized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014476">
        <property type="entry name" value="AHL15-29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31100">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31100:SF70">
        <property type="entry name" value="OS07G0235200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016021">
        <property type="entry name" value="ESCAROLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PPC" evidence="4">
        <location>
            <begin position="59"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR016021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ91641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="262" mass="26555" checksum="9AD9E88645FE4492" modified="2012-06-13" version="1">MAAKQADGEHSGGGISGHDDDPEPKEGAVVVPANRRPRGRPPGSKNKPKPPIFVTRDSPNALRSHVMEVSSGADIADSIAHFSRRRQRGVCVLSGAGAVADVALRQPAAPGGAVVALRGRFEILSLTGTFLPGPSPPGSTGLTVYLAGGQGQVVGGSVVGTLTAAGPVMVIASTFANATYERLPLDDEAEEDRHELAGRGVPGSTGQMAPGVPPMMMGDHSAGMSMYGLPASLMPGGGGGHAQAAAEQGINAWAPQHARPPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITG0</accession>
    <name>I1ITG0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100846850</name>
        <name evidence="1" type="ORF">BRADI_4g39750v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Poaceae</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ91786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblPlants" id="KQJ91786">
        <property type="protein sequence ID" value="KQJ91786"/>
        <property type="gene ID" value="BRADI_4g39750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846850"/>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="171" mass="18993" checksum="9FDA951515C5164D" modified="2012-06-13" version="1">MSRDPLIVGNIVGDIVDYFDASARLRVLYGNREITNGSELKPVANQPTVQITGRSRSLYTLVIMDPDAPTPSDPSKREYLHWLVTDIPEGGDVSRGTAVVAYEKPQPTAGIHRFAFVAFRQTERQTIYAPGWRANFNARDFAECYGLGAPVAAAYFNCQREGTCGGRRYRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodium</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IUB7"/>
    <dbReference type="STRING" id="15368.I1IUB7"/>
    <dbReference type="EnsemblPlants" id="KQJ92244">
        <property type="protein sequence ID" value="KQJ92244"/>
        <property type="gene ID" value="BRADI_4g42450v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92244">
        <property type="protein sequence ID" value="KQJ92244"/>
        <property type="gene ID" value="BRADI_4g42450v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S9AF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="I1IUB7"/>
    <dbReference type="OMA" id="CSECRCC"/>
    <dbReference type="OrthoDB" id="768311at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="InterPro" id="IPR057188">
        <property type="entry name" value="DUF7866"/>
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        <property type="entry name" value="BOWMAN-BIRK SERINE PROTEASE INHIBITORS FAMILY DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
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    </dbReference>
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        <location>
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        </location>
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        <location>
            <begin position="26"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7866" evidence="2">
        <location>
            <begin position="53"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="132" mass="13834" checksum="A18577A9E202B31C" modified="2012-06-13" version="1">MAFVHLAGLALLLLLQVAAQGGAMATDVSGEYVPVPRVAYRNLSSSLMAGSTPFETCKDCKCCQPPAPGVSSNKFNTTGQTCVNTKCCYKINCNLPGKPFGTCAFTPIKCGCSSDNDCPAKQSSSLKLQELT</sequence>
</entry>
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    <accession>I1IV16</accession>
    <name>I1IV16_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase-related protein</fullName>
        </recommendedName>
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    <gene>
        <name evidence="3" type="primary">LOC100829146</name>
        <name evidence="2" type="ORF">BRADI_4g44470v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ92561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="protein sequence ID" value="KQJ92561"/>
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            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ92561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92561"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="9962" checksum="CD492C72E35BE4C4" modified="2012-06-13" version="1">MCPLRVILIFLSATVAGFFLLRGLNAGGNEFLDDDCSSEPESPRAAAAAVPLRSKVGSAVRTGFWTTVDMASGRYLWRTLVAPPSDSESDKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVA6</accession>
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        <recommendedName>
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    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577175.2">
        <property type="nucleotide sequence ID" value="XM_003577127.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVA6"/>
    <dbReference type="FunCoup" id="I1IVA6">
        <property type="interactions" value="1723"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IVA6"/>
    <dbReference type="EnsemblPlants" id="KQJ92680">
        <property type="protein sequence ID" value="KQJ92680"/>
        <property type="gene ID" value="BRADI_4g45220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828935"/>
    <dbReference type="Gramene" id="KQJ92680">
        <property type="protein sequence ID" value="KQJ92680"/>
        <property type="gene ID" value="BRADI_4g45220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828935"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022641_0_0_1"/>
    <dbReference type="OMA" id="VCQLSYQ"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46598">
        <property type="entry name" value="BNAC05G43320D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46598:SF5">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="207"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="244"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="363"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="403"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="438"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="476"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="511"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="547"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ92680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="78549" checksum="C490F8059D8A4AAD" modified="2012-06-13" version="1">MMMLQWHGGKLNLQPQPLPPPAPPPSFFPPSSSSSRAHNHLLHSAISPAPQLSPTHDGDGDENDDALLALLRAGDTDAAYKLFAASPTLPGSPAAASRLLAQLSYTSPRTFPRAADLLRRLRAEGSLGLLDANSLSLAASAAARSGHARLACSLLLSMLRRGLLPDRRAYTAAVSRLSPPSRALRLFDAVLHHLRRTTDAPASSLPDTAAFNAALSACADAGDCRRFRRLFDEMRAWGAAAEPDVVTYNVAIKMCARAGRKDLVARVLPRILAAGLAPDATTFHSIVAAYVGLRDIPAAEAVVQAMRDRRADLCLLLRQLPSSPSSSSDADEHSHVLEDIVVGDDGQGTEKAPLLLPRTYPPDSRVYTTLMKGYMNAGRVDDVVAMARAMRREGETMPASKPDHVTYTTVMSTLVAAGDVGRAHALLDDMAGAGVPASRVTYNVLIKGYCQQLQMSKARELLQEMMSADGGGIEPDVVTYNTLMDGCVLADDSAGALALFNEMRSRGVAPSTASYTTLMKAFAAAGQPRAVQRVFDEMDRDPNAAPDRAAWNMLVEGYCQQGHLESAKAATEKMKERGVQPDVATFGSLAKGVAAARKPGEALVLWGEVKARRDAGELRPDEELLDALADVCVRGAFFKKALEIVACMEENGIAPNKTKYKKIYIEMHSRMFTSKHASQARQDRRRERKRAAEAFKFWLGLPNSYYGSEWRVGPLDSNSEDDDGNYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVW5</accession>
    <name>I1IVW5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Arginase 1, mitochondrial</fullName>
            <ecNumber evidence="3">3.5.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Arginine amidohydrolase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100821269</name>
        <name evidence="14" type="ORF">BRADI_5g02160v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydrolysis of L-arginine to urea and L-ornithine. The latter can be utilized in the urea cycle or as a precursor for the synthesis of both polyamines and proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-arginine + H2O = urea + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:20569"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="EC" id="3.5.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="12">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580734.1">
        <property type="nucleotide sequence ID" value="XM_003580686.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVW5"/>
    <dbReference type="FunCoup" id="I1IVW5">
        <property type="interactions" value="1356"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IVW5"/>
    <dbReference type="EnsemblPlants" id="KQJ81663">
        <property type="protein sequence ID" value="KQJ81663"/>
        <property type="gene ID" value="BRADI_5g02160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821269"/>
    <dbReference type="Gramene" id="KQJ81663">
        <property type="protein sequence ID" value="KQJ81663"/>
        <property type="gene ID" value="BRADI_5g02160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821269"/>
    <dbReference type="eggNOG" id="KOG2964">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_3_0_1"/>
    <dbReference type="OMA" id="CIDAGFV"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004053">
        <property type="term" value="F:arginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11593">
        <property type="entry name" value="Agmatinase-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000007">
        <property type="entry name" value="Arginase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 12" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ81663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="342" mass="36950" checksum="07F60D2A26EF610F" modified="2012-06-13" version="1">MGAAAAAAAAGAAKWIQRLGAAGISTEALERGQSRVIDASLTLIRERARLKGELLRSMGDVKASASLLGVPLGHNSSFLQGPAFAPPRIREAIWCGSTNSSTEEGKELDDPRVLTDVGDVPIQEIRDCGVGDDRLMHVISESVKTVMEEDPLRPLVLGGDHSISYPVVRAVSEMLGGPVDILHLDAHPDIYDCFEDNPYSHASSFARIMEGGHARRLLQVGLRSITKEGREQGKRFGVEQYEMRTFSRDREKLENLKLGEGVKGVYISVDVDCLDPAFAPGVSHIEPGGLSFRDVLNILQNLQGDVVAADVVEFNPQRDTVDGMTAMVAAKLVRELTAKISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IY73</accession>
    <name>I1IY73_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100830579</name>
        <name evidence="2" type="ORF">BRADI_5g11360v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579846.1">
        <property type="nucleotide sequence ID" value="XM_003579798.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IY73"/>
    <dbReference type="EnsemblPlants" id="KQJ82847">
        <property type="protein sequence ID" value="KQJ82847"/>
        <property type="gene ID" value="BRADI_5g11360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830579"/>
    <dbReference type="Gramene" id="KQJ82847">
        <property type="protein sequence ID" value="KQJ82847"/>
        <property type="gene ID" value="BRADI_5g11360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830579"/>
    <dbReference type="eggNOG" id="ENOG502QRI5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036032_3_0_1"/>
    <dbReference type="OMA" id="PLYLKGH"/>
    <dbReference type="OrthoDB" id="1946at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IY73">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007434">
        <property type="entry name" value="FemAB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47017">
        <property type="entry name" value="ACYL-COA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47017:SF1">
        <property type="entry name" value="ACYL-COA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04339">
        <property type="entry name" value="FemAB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ82847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="480" mass="54620" checksum="B0EA0B18BC47ED6D" modified="2012-06-13" version="1">MASPAVAMRRPSPTVRASAPRRRLRRHGYSPCNLGFQSSASRLKVTALFGWIKGDTRTRELIPSAESYTLTGSASEVDMKPREVSISVVSSIMDIPPAEWDACAVDSAEPEKLNPFLTHAFLSSLEESGSAVKETGWLPLHVVARDEVENIIGVVPLYLKSHSRGEFVFDQSWAEAYYSYGLEYYPKLQSCVPFTPVTGQRILLRDTPYQDQVFDALVKALKNLATKLKVSSLHITFPSEGEFGKLKCNGLMQRIGMQYHWRNRNYKSFDDFLMDLKQPKRKNIRQERKKIPAQNLKMKRLRGDEIKSSHWDTFYKFYRNTTDNHWGRPYLTREFFHLLGEKMGDKVMLIVAEHDDKLVAGALNLIGGDTLFGRLWGCLPDAYFPNLHFEACYYQAIEAAIELNLSKVEAGAQGEHKIQRGYLPVTTYSCHYFLEPGFATAIGNFLERETAQVKHVVKVLHDSGPYKEEILKEFAPSDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1J005</accession>
    <name>I1J005_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Short-chain dehydrogenase/reductase</fullName>
            <ecNumber evidence="5">1.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC112269522</name>
        <name evidence="6" type="ORF">BRADI_5g16650v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312157.1">
        <property type="nucleotide sequence ID" value="XM_024456389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J005"/>
    <dbReference type="STRING" id="15368.I1J005"/>
    <dbReference type="EnsemblPlants" id="KQJ83750">
        <property type="protein sequence ID" value="KQJ83750"/>
        <property type="gene ID" value="BRADI_5g16650v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112269522"/>
    <dbReference type="Gramene" id="KQJ83750">
        <property type="protein sequence ID" value="KQJ83750"/>
        <property type="gene ID" value="BRADI_5g16650v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_9_0_1"/>
    <dbReference type="OMA" id="WMWKNCR"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J005">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="(+)-neomenthol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490">
        <property type="entry name" value="(+)-NEOMENTHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490:SF78">
        <property type="entry name" value="OS04G0532100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GDHRDH"/>
    </dbReference>
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        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ83750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="309" mass="34035" checksum="561A7927A49E06BD" modified="2012-06-13" version="1">MEGAIPISWNTRIAVVTGGSKGIGLEVCKQLAGSGITVVLTARDETRGTAAVEQIVRLGHSDVIFHQLDITDALSIARLTDFLKARFGKLDILVNNAATDGIEQVLDPVYGSIPGDEKFDGMDAYQRIDWMWANCRETYETAKQGLQTNYYGTKRVTEALLPLLQSSSDGRIVNVSSNFGLLSLFRNEELKQELNDVERLTEERLDELLAIFLQDFEAGAAEARGWPAEFSAYKVAKAAMNAYSRILAKRHPELRLNCAHPGYVRTDITRNSGILTPEEGARNVVKVALLPEDGPTGVYFHEGQEASFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E4L3</accession>
    <name>A0A0Q3E4L3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g10867v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82767.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E4L3"/>
    <dbReference type="EnsemblPlants" id="KQJ82767">
        <property type="protein sequence ID" value="KQJ82767"/>
        <property type="gene ID" value="BRADI_5g10867v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82767">
        <property type="protein sequence ID" value="KQJ82767"/>
        <property type="gene ID" value="BRADI_5g10867v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E4L3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ82767.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="210" mass="22692" checksum="DD5EF38428A877F4" modified="2018-03-28" version="2">MAWGLPPSTSPLPALPPSPAPARPAAAYPNGDRRGNVCCPMSWYFLCPHTKNPDPPIQSHSSRRKPTYEKSPHRKKKTTPMPLAWSSRTASSRRRRNGPRAVRRRAASRMVADPNGRRRQRDPTSPRTHPRPPAGGRIRSQPPCGSRPPGSSSSHTRLPSPVALGHGLATAGSPSGRPSVADSASPTFPAQKASPDSQYGHRRQRAAPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E5D4</accession>
    <name>A0A0Q3E5D4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_5g00660v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81428.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E5D4"/>
    <dbReference type="FunCoup" id="A0A0Q3E5D4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3E5D4"/>
    <dbReference type="EnsemblPlants" id="KQJ81428">
        <property type="protein sequence ID" value="KQJ81428"/>
        <property type="gene ID" value="BRADI_5g00660v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81428">
        <property type="protein sequence ID" value="KQJ81428"/>
        <property type="gene ID" value="BRADI_5g00660v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E5D4"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3E5D4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ81428.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="146" mass="15881" checksum="85EE4C9EE1974EA7" modified="2018-03-28" version="1" fragment="single">PDRPDEMQRITEAGGWVIFHNGATRVRGILAMSRAIGHRILKPEVICDPEIEITTRTEGDDCLILASDGVWDVVSNQMACEVVRQCLDDGTPPDLHLPAAPAPAEGQHQQSSRRCNVAAAALGRFALGRESSDNISAIVIDLKMRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E5P0</accession>
    <name>A0A0Q3E5P0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g13602v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E5P0"/>
    <dbReference type="EnsemblPlants" id="KQJ83183">
        <property type="protein sequence ID" value="KQJ83183"/>
        <property type="gene ID" value="BRADI_5g13602v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83183">
        <property type="protein sequence ID" value="KQJ83183"/>
        <property type="gene ID" value="BRADI_5g13602v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E5P0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ83183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="97" mass="11144" checksum="BDADFCD9F570E809" modified="2016-01-20" version="1">MRSRGSRRRGCVKRCPGSSSPCRGRRGVPPPGSTRCSSGRRQRTSLSSHPSANGRCRQLARRCSCRLSRRWPAWRGRSRRRASGRRCWRSSRPRPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EMT5</accession>
    <name>A0A0Q3EMT5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g21731v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EMT5"/>
    <dbReference type="EnsemblPlants" id="KQJ88846">
        <property type="protein sequence ID" value="KQJ88846"/>
        <property type="gene ID" value="BRADI_4g21731v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88846">
        <property type="protein sequence ID" value="KQJ88846"/>
        <property type="gene ID" value="BRADI_4g21731v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EMT5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EMT5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ88846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="96" mass="10364" checksum="1DB072C4DC634AF7" modified="2016-01-20" version="1">MLDYVKATLHNMEQQGEDSDRRGRQGVPGGELGGSETSASVVDGWGTSSSICDMARDGEGDKESSGGLFWAIFLKFSYWTPDWASLGDALSELQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EU81</accession>
    <name>A0A0Q3EU81_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-acylglycerol-3-phosphate O-acyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.51</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Lipid droplet-binding protein CGI-58 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100835470</name>
        <name evidence="14" type="ORF">BRADI_4g35382v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578450.1">
        <property type="nucleotide sequence ID" value="XM_003578402.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EU81"/>
    <dbReference type="STRING" id="15368.A0A0Q3EU81"/>
    <dbReference type="EnsemblPlants" id="KQJ91078">
        <property type="protein sequence ID" value="KQJ91078"/>
        <property type="gene ID" value="BRADI_4g35382v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835470"/>
    <dbReference type="Gramene" id="KQJ91078">
        <property type="protein sequence ID" value="KQJ91078"/>
        <property type="gene ID" value="BRADI_4g35382v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835470"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EU81">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055089">
        <property type="term" value="P:fatty acid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070328">
        <property type="term" value="P:triglyceride homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="entry name" value="Probable 1-acylglycerol-3-phosphate O-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="13">
        <location>
            <begin position="116"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075419"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ91078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="413" mass="45854" checksum="7AB7243CF2BC167F" modified="2016-01-20" version="1">MRRAAAVTRMAATEEMRQASAAAAATVTEASASAAPPAGSRWARVWPAALRWIPTSTERIIAAEKRLLSVLKTGYVQEQVNIGSAPPGSKVRWFRSSSDEPRFINTVTFDSKENAPTLVMVHGYGASQGFFFRNFDALASRFRVIAIDQLGWGGSSRPDFTCKSTEETEAWFIDSLEEWRKAKNLSNFILLGHSFGGYVAAKYALQHPEHVQHLILVGSAGFSSETDHSSEWLTKFRATWKGMLVNQLWESNFTPQRIVRGLGPWGPDLVRRYTTARFGSYSTGELLTEHESGLLTDYIYHTLAAKASGELCLKYIFSLGAFARKPLLQSASDWKVPTTFIYGHDDWMKYQGAQQARKDMKVPCEIIRVPQGGHFVFIDNPSGFHSAVFYACRKFLSGDAEEGLSLPDGLISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EUY3</accession>
    <name>A0A0Q3EUY3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Auxin induced protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100845543</name>
        <name evidence="2" type="ORF">BRADI_4g36956v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblPlants" id="KQJ91310">
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        <property type="gene ID" value="BRADI_4g36956v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845543"/>
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        <property type="protein sequence ID" value="KQJ91310"/>
        <property type="gene ID" value="BRADI_4g36956v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="619837at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="P:response to auxin"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AUXIN INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31175">
        <property type="entry name" value="AUXIN-RESPONSIVE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ91310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="14594" checksum="3C33846E65CB5E1E" modified="2016-01-20" version="1">MIHPRKLAQLAKKLQRKVAAGAGGQQADSECCSTALVADKGHCVVYTADGARFEVPLAYLGTTVFSELLRMSGEEFGFASGGERITLPCDAMAMEYVLCLVRREASKEVERAFLSSIAGHCVAPSMGLHHQFALCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EYB6</accession>
    <name>A0A0Q3EYB6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Molybdenum cofactor sulfurase middle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g37993v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_4g37993v3"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="entry name" value="MITOCHONDRIAL AMIDOXIME REDUCING COMPONENT 1"/>
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    </dbReference>
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        <location>
            <begin position="25"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ91484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="185" mass="20033" checksum="844B41A2D9DAAA83" modified="2016-01-20" version="1">MEKAASFLSSLLGTGGGTDEEPAAAAVKSVLIYPIKSCRGISVPQAPITSTGFRWDRQWAVVNAKGRACTQRVEPKMALVEVEMPPGAFDEDWQPTQDSYMVIRAPGMDALKVLLSAELPTVDDISVWSGLVLHMMKETTQPNGSPPILGTQVDLCGLKKSLKSDRLILTMLKVTKLCFQMASHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FQY2</accession>
    <name>A0A0Q3FQY2_BRADI</name>
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        <recommendedName>
            <fullName evidence="9">Protein DCL, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100833797</name>
        <name evidence="6" type="ORF">BRADI_3g57980v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="KQK01752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_3g57980v3"/>
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        <property type="gene ID" value="BRADI_3g57980v3"/>
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        <property type="component" value="Chromosome 3"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:chloroplast organization"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26279" checksum="1A5D0E23A88460A3" modified="2016-01-20" version="1">MHFPSPRARPMAVVAVAAPAVSCLRLVLPGFGGLVSASLLASPCRGRGGVAVRSRGQGAAPPDPAAILRRPAAATTSEEEEVEERESDAVSALGSPVEDEPPEEGRRKGPEREWVDWEDLILEDTVPLVGFVRMILHSGKYTSGDRLTPEHEKAILERLLPYHPQYEKKIGCGIDYITLGLHPEFENSRCLFIVRKDGEEVDFSFWKCIKGLIRKKYPLYADSFILRHFRRRQDY</sequence>
</entry>
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    <accession>A0A0Q3GD64</accession>
    <name>A0A0Q3GD64_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC104582798</name>
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    </gene>
    <organism evidence="1">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
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        <property type="gene ID" value="BRADI_2g41856v3"/>
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    <dbReference type="KEGG" id="bdi:104582798"/>
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        <property type="component" value="Chromosome 2"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="136" mass="15538" checksum="FECCADD7CE65611A" modified="2016-01-20" version="1">MAPLLPLRSLMHAPSSGRRPLLSIPEAAITGDPLQIRLGRDCEFPGYNAFRRFRVEVAMERVELLHLIADLVEAIFYADNPEWRFNIIKHHKEQGMFGAKNTSLYRKCVDEQLAKDLQLVDSTAETAPNFPFLLFL</sequence>
</entry>
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQK11007">
        <property type="protein sequence ID" value="KQK11007"/>
        <property type="gene ID" value="BRADI_2g57558v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11007">
        <property type="protein sequence ID" value="KQK11007"/>
        <property type="gene ID" value="BRADI_2g57558v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GIQ6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GIQ6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK11007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="278" mass="29249" checksum="74CA8A1F3747AEE6" modified="2016-01-20" version="1">MATAAARDGGGAAVDLGTRGHGEPEARTPTTTLLADAPPLAVAGCRIRDLRSRGLRIHPGPDLVYSSAADASCLPRRGLRPRDSKMSTESISARAASPPAADKLSGDPPSQTSISIASSSSGSKMSPESTSTKGISPPATCSPESAEDLPEIQGVRESNETEADHERTSSWAAAFGSFLRRSSWTVKPRYKRPMHSLANKEDANDCPETIPLLSHADVVDLHRHATELSELSEHPALVAPAAYLAKALGVLEDGDSSARSLGELKQRPPWPELPPTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GL05</accession>
    <name>A0A0Q3GL05_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100837781</name>
        <name evidence="13" type="ORF">BRADI_2g58261v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase HD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232716.1">
        <property type="nucleotide sequence ID" value="XM_010234414.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GL05"/>
    <dbReference type="STRING" id="15368.A0A0Q3GL05"/>
    <dbReference type="EnsemblPlants" id="KQK11125">
        <property type="protein sequence ID" value="KQK11125"/>
        <property type="gene ID" value="BRADI_2g58261v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837781"/>
    <dbReference type="Gramene" id="KQK11125">
        <property type="protein sequence ID" value="KQK11125"/>
        <property type="gene ID" value="BRADI_2g58261v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837781"/>
    <dbReference type="OrthoDB" id="2019803at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GL05">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.340:FF:000004">
        <property type="entry name" value="Histone deacetylase HDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="120"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="145"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK11125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="302" mass="32248" checksum="2A20CBFFE8CEEDF4" modified="2016-01-20" version="1">MTMIKFWGVEVKPGQTVSCDGGHDHIIHVSQAALVETKKGSGNVVVSTKIDDQKVIIGTLSAENHPQILCDLNFQKKFELSHNSETASVFVCGYQSFMPDEFDPDSSEDEDEVEAVNNQVINNVGGIHEVTAPAKDGKKGTDGNGSDDDSDDDLLDLLSSGDDEMTDDDSSSEEDDTTSEEDDTSSEEDDISSEESSSDEEDEETLKEPEISKKRVAATALKAPASDKKAKIATPSGQKTASDDKKATHVTTPHPAKQAKTPANSKPKKSPKSTGGTVACKSCSKTFGSDAALKSHEKAKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GYZ4</accession>
    <name>A0A0Q3GYZ4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100827628</name>
        <name evidence="13" type="ORF">BRADI_4g03440v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318512.1">
        <property type="nucleotide sequence ID" value="XM_024462744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GYZ4"/>
    <dbReference type="EnsemblPlants" id="KQJ86122">
        <property type="protein sequence ID" value="KQJ86122"/>
        <property type="gene ID" value="BRADI_4g03440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827628"/>
    <dbReference type="Gramene" id="KQJ86122">
        <property type="protein sequence ID" value="KQJ86122"/>
        <property type="gene ID" value="BRADI_4g03440v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="666026at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GYZ4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000081">
        <property type="entry name" value="Cytochrome P450 CYP81N5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF62">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ86122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="57505" checksum="35A99A93EABD18A2" modified="2016-01-20" version="1">MPMPMPPEAMDAATAGISGVLLLLLLLLAGALAVFARLSLGSRSSSSSAPSPASSLPVLGHLHLLKKPLHRSLAALAAQAPPMPLASLRLGARRALLVSTHAAAEECFTGARDAALAGRPRLLAGKHLGYGHSTVVWASHGAHWRGLRRFLATELFSASRLAALAAGRQAEAAALVRNLLLDSSAAAAGAGGGDGEEICAAVALRPRLFELVLNVMLRAVTGHAGGVGIGDDGCVRRRRVAFQEIVEESFAVSGAPSFGDFFPALRWVDRLRGVEAALAGLQRKRDAFVAGLIDEHRRIPGRDVDEKKKKGVIDVLLEQQEADPEQYTDTIIKGIVLVLLSAGTDTSALTTEWAMALLLTHPEAMEKARAEIDGNIGTGRLVEEPDISNLPYLQCVVKETLRLCPVAPVVPAHEAMEDCVVGGFRVRRGTIVLVNAWAIHRDADVWDAPGEFRPERFLDDADIGMGNAAPMLPFGLGRRRCPGEGLAVRLVSLALAAMVQCFEWDVVGDVDMAEGAGLTMPMATPLAAVCRPRAFVESLLSAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H8X8</accession>
    <name>A0A0Q3H8X8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100834888</name>
        <name evidence="4" type="ORF">BRADI_1g46460v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564137.2">
        <property type="nucleotide sequence ID" value="XM_003564089.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H8X8"/>
    <dbReference type="STRING" id="15368.A0A0Q3H8X8"/>
    <dbReference type="EnsemblPlants" id="KQK19117">
        <property type="protein sequence ID" value="KQK19117"/>
        <property type="gene ID" value="BRADI_1g46460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834888"/>
    <dbReference type="Gramene" id="KQK19117">
        <property type="protein sequence ID" value="KQK19117"/>
        <property type="gene ID" value="BRADI_1g46460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834888"/>
    <dbReference type="OrthoDB" id="691484at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H8X8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051992">
        <property type="entry name" value="OxStress_Response_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33172:SF11">
        <property type="entry name" value="DUF3741 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33172">
        <property type="entry name" value="OS08G0516900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="13"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK19117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="240" mass="25775" checksum="626E2B9575DEE77B" modified="2016-01-20" version="1">MRVGRAMPIALESGARFGRGAVPHQAPLPACGGGTSSAGRGSDSSAAAVHREESEEGDGEVQSSYRGPLETMDALQDALPRRREVSKPNNSKLSSLVNAGDDMASFQPAKDIANTENPSPKKRKGLLPFGINKNELQSKKLSPVGDVTNSTTNCRKSLYPAVTSSSPSKSRRDDDHVCCKNLPCHCLQRKFGAMDPFASPPVTLETQLISVQMRSVSTVDLQDVVESTAEVSPREKRRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HJ47</accession>
    <name>A0A0Q3HJ47_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BRADI_3g04350v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HJ47"/>
    <dbReference type="STRING" id="15368.A0A0Q3HJ47"/>
    <dbReference type="EnsemblPlants" id="KQJ93402">
        <property type="protein sequence ID" value="KQJ93402"/>
        <property type="gene ID" value="BRADI_3g04350v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93402">
        <property type="protein sequence ID" value="KQJ93402"/>
        <property type="gene ID" value="BRADI_3g04350v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HJ47"/>
    <dbReference type="OrthoDB" id="1740823at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HJ47">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000403">
        <property type="entry name" value="Receptor-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000530">
        <property type="entry name" value="Receptor-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48052:SF97">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48052">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033725102" evidence="13">
        <location>
            <begin position="26"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="619"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="29"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="208"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQJ93402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="645" mass="69740" checksum="43D1D05D2D3D744C" modified="2016-01-20" version="1">MCSITVPPVVLQLLLLLSLASPATSCTEEEKRPLLQFLAGLSRDGGLAVSWRDDTDCCEWEGITCNGDGAVTELSLASKGLEGHITPFLANLTSLLCVDLSHNSFSGGLPPELMFSTSIIVLNVSFNQLYEALHELQPSVTTSRPLQVLNISSKQFSSEFPSATWKVMKNLIALNASNNSFTGHVPSSICLGSPSLSLLDLCYNKLSGDIPTTFGNCSKLKVLKAGHNNLSGILPVEIFRATSLEYLSFPNNGLQGELDGAHIVKLSNLATLDLGGNHFTGNIPQSIGQLKRLRELHLGCNNISGEVPSALSNCTHLISIVLNLNNFSGDLSKVNFSTLNSLKSLDLMGNTLSGIFPESIYTCSNLTALRLSTNHFHGEISSRIGNLKHLSFLSLADNSFTNITQAFHALKRCRNISALLIGGNFMNEAMPQDETIDSFQNLQMQMPMLKSNKTGIYLDPILLDLDLPIYQAPSLQYRIGSAWPKGLNLGQNKFTGVIPSEIGQLKELLYLNLSFNNLYGGIPQSICNLMNLQGLDLSNNHLTGAIPAALENLNFLSQFNVSNNDLEGPIPTSGQLSTFQNSSFDGNPKLCGPMLMHHCGSVEAAPVSIISAKQCSNEVIFAISFGVFFGVGVLYDQLVLSRYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HNQ6</accession>
    <name>A0A0Q3HNQ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_3g14695v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HNQ6"/>
    <dbReference type="STRING" id="15368.A0A0Q3HNQ6"/>
    <dbReference type="EnsemblPlants" id="KQJ95018">
        <property type="protein sequence ID" value="KQJ95018"/>
        <property type="gene ID" value="BRADI_3g14695v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95018">
        <property type="protein sequence ID" value="KQJ95018"/>
        <property type="gene ID" value="BRADI_3g14695v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HNQ6"/>
    <dbReference type="OrthoDB" id="687020at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF466">
        <property type="entry name" value="OS08G0138100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033725105" description="Phytocyanin domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="3">
        <location>
            <begin position="18"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ95018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="149" mass="15469" checksum="071AE219B346D127" modified="2016-01-20" version="1">MAITKAIFLIMVTASALFKLHGGAPAGSWDLKTNYTQWAFGLRFFPGDSLRFQYPAATHNVLEVTKAAYDTYNTSVSSSGNSSAVVIATYQTGNDVILLAASGVTRYFVCGFLISAAAGAVPGAGTRGQTNQVHPAGAGFSSGELGHPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IDM3</accession>
    <name>A0A0Q3IDM3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100842816</name>
        <name evidence="10" type="ORF">BRADI_5g19380v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580392.1">
        <property type="nucleotide sequence ID" value="XM_003580344.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IDM3"/>
    <dbReference type="STRING" id="15368.A0A0Q3IDM3"/>
    <dbReference type="EnsemblPlants" id="KQJ84208">
        <property type="protein sequence ID" value="KQJ84208"/>
        <property type="gene ID" value="BRADI_5g19380v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842816"/>
    <dbReference type="Gramene" id="KQJ84208">
        <property type="protein sequence ID" value="KQJ84208"/>
        <property type="gene ID" value="BRADI_5g19380v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842816"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IDM3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000023">
        <property type="entry name" value="protein REVEILLE 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF171">
        <property type="entry name" value="OS04G0583900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="54"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="57"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ84208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="447" mass="49461" checksum="4AE8723B2598B27E" modified="2016-01-20" version="1">MACFEQTKAIDDQRPGAAQVGHQNITEDLRNPQNSSDIDMLAEARVPKARKPYMITKQREKWTEDEHKLFLEAMHLHGRAWRRIQEHIGTKTAVQIRSHAQKFFSKVIRESSGDSSNSSGAAAPAIQIPPPRPKRKSVHPYPCNMRSASGNLARELPRLQKPQLQMQQTLCEQVNGSPTSVVTASLIGSENFDSDTSTIDIEERCPTSSIATDEFAVQVPPTDAEEVKGSNTSKEVVCDTSEAPVLKLFGKRVVVNDSHQRPNLCNMQTVTEMELDASAETTTSGTGKFSSHGASEENTWNPWLTNMQQFMCYLPQGAVFFSYNDGSVPYPQLSNPKPVTSDQQHQQQPSEAEYKLTRREASLAESNTTSSSVPETTTQCSDYAESCTKANGDDDTIVPVAGFRKYVSPNSIHQRGFMPYKRCAAESKVQPQAPGEEADGEMTRLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3III9</accession>
    <name>A0A0Q3III9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100846151</name>
        <name evidence="13" type="ORF">BRADI_4g02930v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <text evidence="10">Belongs to the G-alpha family. XLG subfamily.</text>
    </comment>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IX46</accession>
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="OS09G0436300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="58184" checksum="29DED583B789F8C3" modified="2016-01-20" version="1">MSTRRRRRAEEPSSSAAAAETTKGGKATWFWEAGGRLVETRRPVTAEPMSGRYAEEAEDEEWIVSDDNALPLIPSSRPLPRRCPKFPDGGSMKEISEWNKACDRVYKLAVNDPVTHPPLLRETKDDFDTSSSSTSDEFYCSSSRHREMILGLARSIVSISSTENDGLPEWNFNGIIIGPSESDERARILTSSETVCDYEGKLNHKLLVRLPSKVIKDGELLFFDHHYGLAVLEISVDMLLQCPPFVSSPDYGDDVFVLTRKEDSSLMTTAGKVLWHNEPYFGRNHYLYLSCDLPERISGALVMDRDGNIAGITFDCPSPKTAVLPSFIIKKLIEMESNFRYMARPEHDLSLRAVQFLDISRKEEILYKHNIDSGYLVDQVKIKSTAEIIGIRRGDVIVSVNGMRSLNMLELEEYFLSLGQQFLEKKIESSKIVLKLKVYDPLNCQEHTLCLPLGFSCLKTVKERVTEKISPAIKGKKMPSGRVLSPRKTGGKDPEDSPPRKKGKIVEGKDAQESLTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JAB3</accession>
    <name>A0A0Q3JAB3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="17 18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100839600</name>
        <name evidence="17" type="ORF">BRADI_2g47171v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232172.1">
        <property type="nucleotide sequence ID" value="XM_010233870.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JAB3"/>
    <dbReference type="STRING" id="15368.A0A0Q3JAB3"/>
    <dbReference type="EnsemblPlants" id="KQK09284">
        <property type="protein sequence ID" value="KQK09284"/>
        <property type="gene ID" value="BRADI_2g47171v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839600"/>
    <dbReference type="Gramene" id="KQK09284">
        <property type="protein sequence ID" value="KQK09284"/>
        <property type="gene ID" value="BRADI_2g47171v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839600"/>
    <dbReference type="OrthoDB" id="6020543at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000590">
        <property type="entry name" value="PR5-like receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044546014" evidence="14">
        <location>
            <begin position="27"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="16">
        <location>
            <begin position="32"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="431"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK09284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="718" mass="77732" checksum="5CDABD2EF80AA7CD" modified="2016-01-20" version="1">MACSRKLLGASSLFFTVSVLAIASHAERPNPVRIAVYWGQNGNEGSLNETCSTGLYAYVNLAFLNVFGGDRAPVLNLAGHCDPASGGCAALATDIAYCQSKGVKVLLSIGGGTGNYGLSSASDARNVSIYLWDNFLGGGSGGSSSRPLGGAQLDGIDFDIDTGRDDYYDDLAKNLFMQYNSTVFQESGGSADQVGVTAGGGFSKSNATGEKKKYMLTAAPQCPYPDASLGRALKTGLFDHVWVQFYNNPACQYTAGDPSALQSAWQQWTAALPSASVFLGLPASPDAAPSGYIDADTLASRVLPVVRRAGNYGGVMLWSRFQDVQTGYSAKLQSVANKSTADGPIGSPPVARSVAASSDTRIRTYVIAGSSSLVGVCLFLLTFFYWYKKYYGIFPCQGGSKNTPRIESFLQKQATSHPKRYSYPEVRRMTNSFAQKLGQGGFGAVYRGKLPDGREIAVKMLKDTKGDGEEFMNEVASISRCSHVNVVTLLGFCLQGKKKALIYEYMINGSLERYTFGNNSIQDPNALNWERLFDIVLGIARGLEYLHRGCNTPIIHFDIKPQNILLDGSFCPKISDFGLAKLCHQKESRISIGGARGTVGYIAPEVLFSRQYGAVSSKSDVYGYGMVVLEMVGARNQINVSTESSTKYFPQWIYDNFDEFCCTTHEIVSSETTELLRKMTIVGLWCIQLTPTYRPSMSKVVDMLESDGKDLQLPPKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JFN1</accession>
    <name>A0A0Q3JFN1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein bS18c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_2g59143v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JFN1"/>
    <dbReference type="STRING" id="15368.A0A0Q3JFN1"/>
    <dbReference type="EnsemblPlants" id="KQK11276">
        <property type="protein sequence ID" value="KQK11276"/>
        <property type="gene ID" value="BRADI_2g59143v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11276">
        <property type="protein sequence ID" value="KQK11276"/>
        <property type="gene ID" value="BRADI_2g59143v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JFN1"/>
    <dbReference type="OrthoDB" id="21463at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_bS18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_bS18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479:SF40">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS18M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
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        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="105"/>
            <end position="115"/>
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    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="115"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00005589"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK11276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="115" mass="13448" checksum="91A555B36541D2DC" modified="2016-01-20" version="1" fragment="single">MQCNASMRTSRSLEESRHEQRSLINEWIPVLDLRGLSISSGGKILSRRINRLTLKQQRLITLAIKQARILSFLPFRNYENEKQFQAQSISIITGSRPRKNRHIPQLTQKYSSNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JPM0</accession>
    <name>A0A0Q3JPM0_BRADI</name>
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        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g49784v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
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        <property type="entry name" value="OS01G0527900 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45224:SF10">
        <property type="entry name" value="OS09G0317700 PROTEIN"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <end position="122"/>
        </location>
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            <begin position="25"/>
            <end position="62"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK19683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="122" mass="13519" checksum="20519FB19CC9F9DB" modified="2016-01-20" version="1">MNSDFGFVDLLGSGSAEYGTGCESFDNASYTVPSGGGRGAGGGGGGDDQDNDDAERGQRRLPWTEADNLRLISARLHNSVDPIDGNRKKGEFYWKKVTKEYNENNPVDRRRKPIHCKDHWVR</sequence>
</entry>
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    <accession>A0A0Q3K9B7</accession>
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        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">LOC100833652</name>
        <name evidence="4" type="ORF">BRADI_1g58340v3</name>
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    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK21045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010230129.3"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3K9B7"/>
    <dbReference type="EnsemblPlants" id="KQK21044">
        <property type="protein sequence ID" value="KQK21044"/>
        <property type="gene ID" value="BRADI_1g58340v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK21045">
        <property type="protein sequence ID" value="KQK21045"/>
        <property type="gene ID" value="BRADI_1g58340v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77147">
        <property type="protein sequence ID" value="PNT77147"/>
        <property type="gene ID" value="BRADI_1g58340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833652"/>
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        <property type="gene ID" value="BRADI_1g58340v3"/>
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        <property type="protein sequence ID" value="KQK21045"/>
        <property type="gene ID" value="BRADI_1g58340v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77147">
        <property type="protein sequence ID" value="PNT77147"/>
        <property type="gene ID" value="BRADI_1g58340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833652"/>
    <dbReference type="OrthoDB" id="1904536at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
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    <dbReference type="ExpressionAtlas" id="A0A0Q3K9B7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="entry name" value="187-kDa microtubule-associated protein AIR9"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.80.10.10:FF:000328">
        <property type="entry name" value="187-kDa microtubule-associated protein AIR9"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR056284">
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    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056287">
        <property type="entry name" value="PH_AIR9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31149:SF11">
        <property type="entry name" value="187-KDA MICROTUBULE-ASSOCIATED PROTEIN AIR9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23197">
        <property type="entry name" value="IG_AIR9"/>
        <property type="match status" value="10"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="456"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="AIR9-like A9" evidence="2">
        <location>
            <begin position="552"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="AIR9-like A9" evidence="2">
        <location>
            <begin position="647"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="AIR9-like A9" evidence="2">
        <location>
            <begin position="742"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="AIR9-like A9" evidence="2">
        <location>
            <begin position="844"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="AIR9-like A9" evidence="2">
        <location>
            <begin position="944"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="domain" description="AIR9-like A9" evidence="2">
        <location>
            <begin position="1038"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="domain" description="AIR9-like A9" evidence="2">
        <location>
            <begin position="1135"/>
            <end position="1222"/>
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    <feature type="domain" description="AIR9-like A9" evidence="2">
        <location>
            <begin position="1247"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="domain" description="AIR9-like A9" evidence="2">
        <location>
            <begin position="1435"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="domain" description="AIR9 PH-like" evidence="3">
        <location>
            <begin position="1550"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
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        <location>
            <begin position="45"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23197"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23218"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK21044.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK21044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1659" mass="181542" checksum="9D5F091992515C28" modified="2016-01-20" version="1">MEAPPEVAKPAGAKLVKPRASGPNGKPVLGTGLSVKKRADGVTSAEVNVSRPSSMKPTSSVNAGSVQRRSSTGTAGKQQENGSSLVAKKASPLLSDGLKKSRPVSAQTVSSRPSLEKRTSLSSERTKVDPVKKPAVKASPTSTLKKVPSLTESSNGGSSSASSRRMASNASLDSPRSSSVTSSVTKKMGSRTSLADKGSSVSSRRKSSTADSRDSRFIMLPQVELKASDDVRLDSRGHRVRSLRQLRLTPKLEFVYLRDNLLSSLEGIEMLSGVKVLDLSFNDFKLPGFEPLGNCKVLQQLYLAGNQITSLASLPAFPNLEFLSVAQNRLKSLCMASQPRLQVLAASRNKISTLKGFPHLPSLENLRVEENPLLEMPHLEAASILLVGPTLKKFNDRDLNPTESEVAKQYPAYTAICIRDGWEFCSPELAADSTFSFLLDQWKNKLPQDCMVKTAYVDHPFEEDPCHCHFNFINLSSEDKLVLKYQWFLGGKTPTGFVPISEELSEVYWPKREDVGRCLKVECTPVLNDAEFPPIFAVSLPVCPGTGCPKIINLIVDGELVEGNVLKGVPEIAWCGGRPGKGVASWLRRTWNGNAALIDGAEGMEYQLTIDDIGSSLVFMYTPVTEEGSKGEPQCAMTDFVKAGTPSVSNVHVVGDVVEDNTIKGNGKYFGGKEGLSKFQWFREKENGGFLLVLSNSTEYTLTKEDVGCRLKYVYTPINLEGQEGKAVCTITDTVKKAPPKVFDLKIVGEAREGSKVYVTATVTGGTEVTSRVQWFKASSSECLNDHELEALCTSKVAKTFRIPLGAVGCYVVAKFTPVAPDGEIGAPAYAILDDVVEMLPPSLNFLTVTGEFSEDQILTASYGYIGGYEGNSLYSWYLHETEDDEGSPVPEASGLLQYHIKREDVGKFVSFKCIPVRNDGIVGEPGIFIGKDRVTPGSPIILSLELNGEIIEGTTMVANRRYWGGEEGDTIFRWVLTSSDGTQKEIEGATSSSYCLKCDDIGFYVSVSCEPVRSDGVHGSLVSTEVIGPITPGPPTCRSLELAGSMVEGGRLTCHAVYTGGIKGNCIQEWFRLHDDGSKSKLIADECLDLTLADVDCRMEVIFTPVREDGLHGSPKCVSSDIILPGEPKGVSLVVPECFEDNEISATKTYFGGKEGTGKYTWFRTKEKLDNLENHLVASCSEAVGETLVYKPSLDDVGLYLILHWVPTRCDGKTGDPLMAITDNPVMAASPSVSEVNLKQTSPDLYSGVGIYYGGYEGSSLHRWYRESSDGIRIWIDGTDSSTYEVTDADYSCRLLFGYTPVRSDGLIGEEKFSEPSDILFPELLKIDTLSFKGNQVERETLTVVETIPSNDTQEHIWSNYKKEIKYQWFASNGSGEDLSFEPLTTQCSRSYKVRFEDIGRCLKCECFVVDVFGRSSEPVSAVTAPILPGIPKIEKLEIEGRGFHTNLYAVRGTYSGGKEGKSKIQWLRSMVGSPDLISIPGEIGKMYEANVDDVGYRLVAIYTPVREDGVEGGPVSASTEPIAVEPEIFKEVKQKLDDGSVKFEVLCDKDRTPKKAQVMGHLERRILEVNRKRIKVVKPGSKTSFPTTEARGTYVPPFHVELYRNDQHRFKIVIDGDNEVDLMVQTRHMRDLVILVIRGLAQKFNSTSLNSLLRIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KAZ6</accession>
    <name>A0A0Q3KAZ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rx N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_2g39560v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KAZ6"/>
    <dbReference type="STRING" id="15368.A0A0Q3KAZ6"/>
    <dbReference type="EnsemblPlants" id="KQK08097">
        <property type="protein sequence ID" value="KQK08097"/>
        <property type="gene ID" value="BRADI_2g39560v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08097">
        <property type="protein sequence ID" value="KQK08097"/>
        <property type="gene ID" value="BRADI_2g39560v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3KAZ6"/>
    <dbReference type="OrthoDB" id="690341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19338:SF58">
        <property type="entry name" value="OS09G0517100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19338">
        <property type="entry name" value="TRANSLOCASE OF INNER MITOCHONDRIAL MEMBRANE 13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="16"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="176"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK08097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="39577" checksum="F903352022C57452" modified="2016-01-20" version="1">MEATALSIGKSVLDGALSYAKSALAEEVALQLGVRRDQIFITNELEMMQAFLMAAHDERDDNKVVKVWVKQVRDVAYDVEDSLQEFAVRLQKQSWWRICRTLLNRRRVAKQMKELRANVEDVNQRNMRYNLIKGSDSKDATYTAAEQSSITSAALFGIDEARRAAKQEKSQVDLVKLINGEEKDLWVIAVWGTRVDVGQTSIIRAVYENPDIRSKFPCRAWVKLMHPFNPKDFAQSLVEQFHSAVGVGLNVLFETEKMPQELTEEFNGYVNKKRYLIVINELSNIEEWDRVKKCFPNNSMGSRIIVSTSRVEVASLCAGQENIVSELKQLTADQNIYAFYEKDYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KNR5</accession>
    <name>A0A0Q3KNR5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YTH domain-containing family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100846773</name>
        <name evidence="4" type="ORF">BRADI_5g01317v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YTHDF family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KNR5"/>
    <dbReference type="STRING" id="15368.A0A0Q3KNR5"/>
    <dbReference type="EnsemblPlants" id="KQJ81541">
        <property type="protein sequence ID" value="KQJ81541"/>
        <property type="gene ID" value="BRADI_5g01317v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81541">
        <property type="protein sequence ID" value="KQJ81541"/>
        <property type="gene ID" value="BRADI_5g01317v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KNR5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF95">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="YTH" evidence="3">
        <location>
            <begin position="498"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ81541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="707" mass="80066" checksum="3623E592FAD84F9A" modified="2016-01-20" version="1">MKEAFDPPASVVTTPENATSSCMRSIMRNDVLAGRDTIPPHRSCMRDSMKCEEDANFLSHANRALSLLDYDAQSSESKYAVAIGSDVYSTEEISPESEYDRQHPYFDLSNSSWENSSSSSEAVARNPDASTHVMEKMRTKSNIFGPGHDYLNSYRTTGPRMRRSYPEAAKVDFSFDCHPEHKYKGSNRFTALPNCNGQSTEHRNEIVELPRGTRYVDETTSLSSRWCFDNGGRGDPSLPRGLMYGDEIPSLSTRRCNGNEALSWHHGSEIPSLSSREGYGDEIPSLSTQKCNGISRSSQWPFGAESPSLSSRQSYGDEIPSLSAQKCNGISYSRQFQYGAESHMFSSRQVYSSEIPALSRRFRYQDTTPLYSGQRCHDAEARRLSSYQQGASHGRGHPSDNYASSLVSNPQIKITTNRHTGTRAWVANRVVNSTNHCRNSKKENPLRNSEDIRDQVCGPRANKLNNATTATTKKDTLSPLVHRDQFNRSDFSVQYEHAKFYMIKSYSEDDIHKGIKYNVWASTPNGNSKLDAAFHEAKNLMKETGSKCPVFLFFSVNTSGQFVGLAEMLGPVDFKKTMEFWQQDKWNGFFPVIWHIVKDIPNRLFKHITLENNDNRPVTFSRDTQEIHLPQGLELLKIFKAYRHVTSILDDFDFYEEKKNSRCAQDGINVDSLHEARLSYFGKDDLKSMGDLEAGMESMNFYESCED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LPU2</accession>
    <name>A0A0Q3LPU2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g11175v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LPU2"/>
    <dbReference type="EnsemblPlants" id="KQJ94549">
        <property type="protein sequence ID" value="KQJ94549"/>
        <property type="gene ID" value="BRADI_3g11175v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ94549">
        <property type="protein sequence ID" value="KQJ94549"/>
        <property type="gene ID" value="BRADI_3g11175v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LPU2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ94549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="70" mass="7831" checksum="7BCEA05AD943C58B" modified="2016-01-20" version="1">MPKALPPPPAVTRPSEHAALPIIISNGGFVEAPHYLCDQKYDAFIKAMSTWSKLLCCMIFRRALCLFFCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LWS9</accession>
    <name>A0A0Q3LWS9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g27128v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ96789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LWS9"/>
    <dbReference type="EnsemblPlants" id="KQJ96789">
        <property type="protein sequence ID" value="KQJ96789"/>
        <property type="gene ID" value="BRADI_3g27128v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96789">
        <property type="protein sequence ID" value="KQJ96789"/>
        <property type="gene ID" value="BRADI_3g27128v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LWS9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ96789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="163" mass="17579" checksum="1FD2B9E5163E10CB" modified="2016-01-20" version="1">MLSYRLCLAAAKTGFETVVGKEKDSDSQKKGKGRTLVPTRSAGSSATHLATLSRDGTGVGATLLLGRPPSPRDVLATSRRDGDNRGLALSPPYGRARWRPASSWISFAWRRPWARLVWISDSRGGALRPRGTAQATENGPPECVLLCVCWVTVNSVLPNLLHT</sequence>
</entry>
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    <accession>A0A0Q3MCJ0</accession>
    <name>A0A0Q3MCJ0_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="1">
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            <taxon>Poaceae</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3MCJ0"/>
    <dbReference type="EnsemblPlants" id="KQK02014">
        <property type="protein sequence ID" value="KQK02014"/>
        <property type="gene ID" value="BRADI_3g59863v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02014">
        <property type="protein sequence ID" value="KQK02014"/>
        <property type="gene ID" value="BRADI_3g59863v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3MCJ0"/>
    <dbReference type="OrthoDB" id="665082at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK02014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="60" mass="6531" checksum="5C9A43729AF5ADBF" modified="2016-01-20" version="1">MCRISCCFGGDGGKEESRGAPAAAAHPRYVPRRGQVLRTALGTLLSCWHRRPARTQPLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3MGE2</accession>
    <name>A0A0Q3MGE2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="primary">LOC100822421</name>
        <name evidence="2" type="ORF">BRADI_2g08360v3</name>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565558.1">
        <property type="nucleotide sequence ID" value="XM_003565510.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3MGE2"/>
    <dbReference type="STRING" id="15368.A0A0Q3MGE2"/>
    <dbReference type="EnsemblPlants" id="KQK03518">
        <property type="protein sequence ID" value="KQK03518"/>
        <property type="gene ID" value="BRADI_2g08360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822421"/>
    <dbReference type="Gramene" id="KQK03518">
        <property type="protein sequence ID" value="KQK03518"/>
        <property type="gene ID" value="BRADI_2g08360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822421"/>
    <dbReference type="OrthoDB" id="589927at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3MGE2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011676">
        <property type="entry name" value="DUF1618"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33086:SF73">
        <property type="entry name" value="OS01G0245901 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33086">
        <property type="entry name" value="OS05G0468200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="194"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07762"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK03518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3MLL7</accession>
    <name>A0A0Q3MLL7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g18358v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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</entry>
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    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Bevan M.W."/>
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        </source>
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    <reference evidence="19" key="3">
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                <consortium name="EnsemblPlants"/>
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        </source>
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            <begin position="24"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="17">
        <location>
            <begin position="24"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="17">
        <location>
            <begin position="136"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="361"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK15886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="672" mass="73115" checksum="ECD83FBD4F1A1378" modified="2016-01-20" version="1">MRMAHRLLLLAGIVALLASPAAGVYPWPICGHSGNFAANSTYQANLARIAATLPKNASSSATLFATAQVGVVPEQVWALGLCRGDSNASSCFTCLDQAFTDLSNACAYTKDGTIYYDSCALHYSNIHFRASDNTEYSTTYRLRNDFNATLEPARFQSVVAALMNATVDYAAYNNSGRMYASGEADFDRELPKVYAWAQCTPDMAPARCRDCLGRIMEALPRQFTSAIGARVLGVRCSYRYETKPFFEGPVMVRLQGTSSASPGAPPAPAPTTVVPNVVTPGTAAPAAQGRKYSVPGMVLIVLLPTIAAINLVACLVLWMRRRPLAEAKQTYPGYSAEAEDIESVDSMLIDISTLRAATGDFAEINKLGEGGFGAVYKGILPDGDEIAVKRLSKSSTQGVGELKNELALVAKLKHKNLVRLVGVCLEQQERLLVYEFVPNRSLDIILFGDTEKREQLDWGKRYKIINGIARGLQYLHEDSQLKVVHRDLKASNILLDTNMNPKISDFGLARLFERDQTQGVTNRVVGTYGYMAPEYVMRGNYSVKSDAFSFGVMVLEIVTGRKNNDCYNSKQSEDLLNTMWEHWTAGTVLETVDPCMRSSFSESDVLRCIHVGLLCVQGNAADRPVMSSVVMMLGSETASLSAPSKPAFYARNPGANPGIASDVSTVSLQDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NLX5</accession>
    <name>A0A0Q3NLX5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100839774</name>
        <name evidence="15" type="ORF">BRADI_1g42737v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Na(+)(in) + H(+)(out) = Na(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560776.1">
        <property type="nucleotide sequence ID" value="XM_003560728.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NLX5"/>
    <dbReference type="FunCoup" id="A0A0Q3NLX5">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3NLX5"/>
    <dbReference type="EnsemblPlants" id="KQK18478">
        <property type="protein sequence ID" value="KQK18478"/>
        <property type="gene ID" value="BRADI_1g42737v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839774"/>
    <dbReference type="Gramene" id="KQK18478">
        <property type="protein sequence ID" value="KQK18478"/>
        <property type="gene ID" value="BRADI_1g42737v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839774"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3NLX5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF179">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="419"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="14">
        <location>
            <begin position="32"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK18478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="528" mass="58138" checksum="FB3E92A6D1E1F04F" modified="2016-01-20" version="1">MAAWWEEIVAAVSTSGPGGSSGTVVSICVFTAVLCLCLVAGHLLEENKWVNESITALIIGCIVGAFIFLLSKGRNSHILRFDEQLFFIYVLPPIIFNAGFQVKKKQFFHNFLTIMSFGVVGVFISVAIVSAGCHWLFPKVGFGKLDAVDYLALGTIFSSTDTVCTLQVISQDETPRLYSLVFGEGVVNDATSVVLFNAIKNLDINRLKRGVVLKVIADFLYLFATSTILGATIGLATAYVLKALYFGRHSTDREVALMALMAYLSYMLAELLSLSGILTVFFCGIVMSHYAWHNVTESSRITTRHIFATLSFIAETFIFLYVGMDALDIDKWKTSQASFKTSIGIFGIIISLVLLGRAAFVFPLSILSNFMSGNSEKAPITFKHQVVIWWAGLMRGAVSIALAYNQFTFSGVTLDPVHAAVITTTIVVVFFTTLVFGFLTRPLISAMLPQQRHREGTGGHITSSNSPKDEFILPFLSDENAPGSESGINQAKRSISMLLERPVHMVHIHWRKFDDKFMRPIFGGPLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PIT8</accession>
    <name>A0A0Q3PIT8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g24583v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KQJ89273"/>
        <property type="gene ID" value="BRADI_4g24583v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ89273"/>
        <property type="gene ID" value="BRADI_4g24583v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3PIT8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ89273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="84" mass="8673" checksum="2CC1AC681BEC1B52" modified="2016-01-20" version="1">MPDPVAMTLDPAAVAVCPSTAGMFLSSLSLSRAGQQIVVAVETRPVLSSLFHAAAVDPRTSARCTGGVSPPWSGYGVSSLWIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S9F6</accession>
    <name>A0A0Q3S9F6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100821675</name>
        <name evidence="5" type="ORF">BRADI_1g61937v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK21610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561618.1">
        <property type="nucleotide sequence ID" value="XM_003561570.4"/>
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        <property type="interactions" value="1764"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3S9F6"/>
    <dbReference type="EnsemblPlants" id="KQK21610">
        <property type="protein sequence ID" value="KQK21610"/>
        <property type="gene ID" value="BRADI_1g61937v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821675"/>
    <dbReference type="Gramene" id="KQK21610">
        <property type="protein sequence ID" value="KQK21610"/>
        <property type="gene ID" value="BRADI_1g61937v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821675"/>
    <dbReference type="OrthoDB" id="62853at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3S9F6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CID_dom"/>
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        <property type="entry name" value="ENTH_VHS"/>
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        <property type="entry name" value="PWWP_dom"/>
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        <property type="entry name" value="HEPATOMA-DERIVED GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550:SF83">
        <property type="entry name" value="OS03G0358000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="25"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="CID" evidence="4">
        <location>
            <begin position="836"/>
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        </location>
    </feature>
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            <begin position="210"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="703"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1028"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1343"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="703"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1070"/>
            <end position="1079"/>
        </location>
    </feature>
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        <location>
            <begin position="1087"/>
            <end position="1103"/>
        </location>
    </feature>
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        <location>
            <begin position="1111"/>
            <end position="1139"/>
        </location>
    </feature>
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            <begin position="1142"/>
            <end position="1153"/>
        </location>
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        <location>
            <begin position="1167"/>
            <end position="1176"/>
        </location>
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        <location>
            <begin position="1231"/>
            <end position="1240"/>
        </location>
    </feature>
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        <location>
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            <end position="1281"/>
        </location>
    </feature>
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            <begin position="1287"/>
            <end position="1321"/>
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        <location>
            <begin position="1349"/>
            <end position="1358"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK21610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1403" mass="153638" checksum="23DE62387F8B5B28" modified="2016-01-20" version="1">MAPARRKRGSSAAAAAAAAAAKWKLGDLVLAKMKGFPAWPAMISEPEQWGLSSVKNKRLVFFYGTKQIAFCNYAELEAFTEEKKRSLLAKRHGKGVDFVRAVDEIIDVYNSLKEDSNNKLDLVGNEVKPENLGDNNSSMDTEGLVNSPNMGRDNKLEDHSVIARVHNLVNTDEPSVMVNGSERCVVNSAPEEPTENVSILDEMRNIPLSASSFSKNKPRDTHLQNCYTRSRAPASRRSRSSLNVEARKSQDSGKLLGETSLASVDLVPDNNKEDSTLHKYAGYDKANSASPSTLDGVWLHSSGTTFNQPGTPGASDSSKKISFTAKLDNTRGSEASQNRASMAQFKSTGASGLPMNSTVIFKRKRKPNGKWIPHSTDCITSNKDDKLPAEFSGNLAGSPNSKNDLNKSDGDEHLPLVKRARVRMGTPQLEKSAVSVEETDVSNNRSGLAAPADHCDMQGTRARPSNDFSVDQSSVVNTIPDQQSILNMPVPSGEGHSVWKSKDYQPKVLTLDVEAALPPSKRLHRALEAMSANAAEIISSLPGETGSKQSILNRCESSENSHSNKMADSAVITSDRSCLTESPRLPGLQSMHSSTRKRILQNKDVAASMKLNDPAFDVTQTISLPDRLSSSLGKSSYNDVTRPVSCNGHTEPIGCPAYHVDGSDSRCSGPVDQPLVSDNNVNSDSVPHGETVLAPAINLGDTTSTSSLATKSSSIQSDADTRTSEVHISSALALKELNHRNLKDRRTSPDSMPMKELIAVAQARRFSRSTSFSDNFLNAKYFAKGLVSTPLKDGQGHLSPPNQIIRSTSTDDSVHSSHPFDSMQMKDMKKIAGNSEASAARIAFGAFLGTLTRTKENIARATRIAIECAKHGIAREAIDIIVERMEKETNLHKRVDLFFLIDSITQCSRNQKGGAGDAYPSLIQVVLPRLLYAAAPPGNSAWENRRQCLKVLKLWLERKTLPEYIIRRHIRELEVINEASFGSSHRPSSTERALNDPLRDNEGMLVDEYGSNAGFHIPNLICTKVLEDEEGSSSEDMSFEAVTPEQDAPVSDDKEESQIPGEKHRHILEEVDGELEMEDVAPPSEAEASTTYQLEQSDTNCTTSEHHPSDSGPPLPDNGPPSSPPLPSSPPPIPPPPPVHIAQSTQLQPTLQMASDPVGPHPPRATYNAQSQQPQSIVEHPRNMNPSVAPLQPPSFCNSGYGAHRNQIAPINPPGSHSSFPTPPAPYHANSYHQHLTESMPNEGYHLQRPPPPPPPNQFPHMPREPHQRPQQWSNNSSSHPESYRYSGHDRDHHRPDSMHHGHDRRDHFNDRGYRHNDRGHHYNGRRHHFDERAVRVLAHHDADRGRFPPFPPVPPIPDHFEASSAPTHYRESSDPPLGPCAGWSVEPPVPHVAGAQGSWKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CH80</accession>
    <name>A0A2K2CH80_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g14518v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CH80"/>
    <dbReference type="EnsemblPlants" id="PNT61378">
        <property type="protein sequence ID" value="PNT61378"/>
        <property type="gene ID" value="BRADI_5g14518v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61378">
        <property type="protein sequence ID" value="PNT61378"/>
        <property type="gene ID" value="BRADI_5g14518v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CH80"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT61378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="192" mass="21175" checksum="2763164234F54342" modified="2018-03-28" version="1">MRGWTRGAGKRQGWDGVITGVFGFTDALSLSRRGDTAARLQASTVQTVLAAQFFGNTQAAAGWEERSGRRPAPEDGRRRTGLGNNGRQRPQTTATTGGSSPGRAAVPGDDDVCRRSRTTAAREEIRRGKGGAERNQWRRRRLKQREEVEEENQRGRRPCSQKTPGYWLGGLFLHAGCNGRCRPRKPNGRKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CL62</accession>
    <name>A0A2K2CL62_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g07933v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Bd21</strain>
        </source>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT63309"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="3">cv. Bd21</strain>
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            <dbReference type="Pfam" id="PF13639"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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            <strain evidence="2 3">Bd21</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT63969"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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        <name evidence="6" type="primary">LOC100844200</name>
        <name evidence="5" type="ORF">BRADI_4g25530v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT64179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT64180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310585.1">
        <property type="nucleotide sequence ID" value="XM_024454817.1"/>
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        <property type="nucleotide sequence ID" value="XM_024454818.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A2K2CQ82"/>
    <dbReference type="SMR" id="A0A2K2CQ82"/>
    <dbReference type="STRING" id="15368.A0A2K2CQ82"/>
    <dbReference type="EnsemblPlants" id="PNT64179">
        <property type="protein sequence ID" value="PNT64179"/>
        <property type="gene ID" value="BRADI_4g25530v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT64180">
        <property type="protein sequence ID" value="PNT64180"/>
        <property type="gene ID" value="BRADI_4g25530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844200"/>
    <dbReference type="Gramene" id="PNT64179">
        <property type="protein sequence ID" value="PNT64179"/>
        <property type="gene ID" value="BRADI_4g25530v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT64180"/>
        <property type="gene ID" value="BRADI_4g25530v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
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    </dbReference>
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        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
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        <property type="term" value="C:preribosome, small subunit precursor"/>
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        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PUM"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="552"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
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            <end position="770"/>
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        <location>
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            <end position="57"/>
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        <location>
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            <end position="686"/>
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        <source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQ95</accession>
    <name>A0A2K2CQ95_BRADI</name>
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        <recommendedName>
            <fullName evidence="8">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">BRADI_4g25457v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23177:SF60">
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        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="21"/>
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        </location>
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        <location>
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            <end position="57"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="437"/>
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        </location>
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        <location>
            <begin position="464"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT64173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="56208" checksum="926AFFA1355CA42A" modified="2018-03-28" version="1">MAEVALFRGPTNLASTASRGSPSPSSSSLSYLSDSDVLQRRSSAGSAEREAGRGSPREEEEERWSFLALLLELLRKSLLRCMADGGGGGSGGGMEIGLPTDVQHVAHVTFDRFHGFLGLPVEFEPEVPRRAPSARLQIDVSVVLNSVVSEDPVFGSELNVNSLKDAIFSLTLLHIWCCLFRASVFGVSTQSMQCSYDSRGNSVPTILLMMQRRLYEQGGLQAEGIFRINAENSQEEFVRDQLNSGNVPDGIDVHCLAGLIKAWFRELPSGVLDSIPPEQVMQCQSEEDCARVAKCLPPTEAALLDWAVNLMADVVQEEQINKMNDRNVAMVFAPNMTQMADPLTALMYAVQVMNFLKMLIQKTLKDREESNLEDISLPQKDSSDENGHQNPSLPLDCQPEQASRRPSFVSEEPVLYSPTHSPEDKPVASTVQKSNVPTSMESSASCSEPAPATADASFATAVNSLQGRGSRSLNSRRARNGKGQSGTRGVTTAEKSRGVSIVSRINSKAERIEAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CTW8</accession>
    <name>A0A2K2CTW8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g42895v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CTW8"/>
    <dbReference type="EnsemblPlants" id="PNT65467">
        <property type="protein sequence ID" value="PNT65467"/>
        <property type="gene ID" value="BRADI_4g42895v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65467">
        <property type="protein sequence ID" value="PNT65467"/>
        <property type="gene ID" value="BRADI_4g42895v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CTW8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT65467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="328" mass="34280" checksum="9EB332D44E7842A7" modified="2018-03-28" version="1">MNVGRRDKVAPRAGSRPKPKAQRRLGLAPPPAGPSWPSPASAAASVDPFAALQHHGRPTAPSPCRLLLLHGSHGSGGGVDHGELLRDEHRASRSSSSSPSRVPLLYPPRAAVPTPSSTTCSYAMVVAAVRPTNAAAEAPAPLRPTSLVPRTCPLHLPAVRLLPPPMLRILGLTCKSTSSARWPAYSPPPSVYPLFPPTIAAEPPRPPVLPRLRPCAPELAGAVPTPSRARGQVSKHLDATVDPRVPSACPLAAPSAPATVTLRRRPEHGDGGAREEVRAREQLPLPHPRVERAAADSATPAVPVPEHCRPSFPGVAGLLPRSARDGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CUJ5</accession>
    <name>A0A2K2CUJ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BRADI_3g01137v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CUJ5"/>
    <dbReference type="FunCoup" id="A0A2K2CUJ5">
        <property type="interactions" value="1244"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CUJ5"/>
    <dbReference type="EnsemblPlants" id="PNT65707">
        <property type="protein sequence ID" value="PNT65707"/>
        <property type="gene ID" value="BRADI_3g01137v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65707">
        <property type="protein sequence ID" value="PNT65707"/>
        <property type="gene ID" value="BRADI_3g01137v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CUJ5"/>
    <dbReference type="OrthoDB" id="1890790at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CUJ5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010089">
        <property type="term" value="P:xylem development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000395">
        <property type="entry name" value="receptor-like serine/threonine-protein kinase NCRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000415">
        <property type="entry name" value="receptor-like serine/threonine-protein kinase NCRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF23">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE NCRK ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033311502" description="non-specific serine/threonine protein kinase" evidence="16">
        <location>
            <begin position="24"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="227"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="525"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="PNT65707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="595" mass="64612" checksum="A03AC0713D090299" modified="2018-03-28" version="1">MTLHMKLALVSLSCVLLIQVTSCDDTAGASEAASWTCVCAAHPLGEPNSNSSQSSSCSSSCHCLQDENGATGSWNCSCTSDKARQEEEHTALHDGGCFTSCNCTSGGSEEGRKPFSSKTVIATLLVCVVLTTIAFVGTTAYYLRRKDALSPHSRMHSFDKYSSWSSRSNLVSHRSSPLPQLKPRPGFSVIKGFLCSCPILCRNESGPFPGIVLRFSYAELEQATGNFSDEHLIGVGGTSKVYRGQLGDGKVVAVKKLRPLRGADEDYEFLSEIELLSRLNHCHVVPLLGYCSESHHGRLLVFELMPNGNLRECLDLKQGRKPMAWQVRVAVALGVARGLEYLHEAAAPRVLHRDIKSTNILLDDKFRAKITDLGMAKCLMSDGVTSCPSSPPPSARTTAMLVGTFGYLAPEYAIVGKASLKSDVFSFGVVVLELITGRQPVVHRSSSANGGGSDESLVLWATPRLGDSRKVVTELPDPALEGQFAAEEMQVMAHLVRECLQWDPEARPSMTEVVQILSTIAPVTGNRRPHLPDNGNFVDNGGERRPQEGNNVVSAPVKTTTTTTSRSWEEEVDLTEPRLEKFTQPTTATSHQLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CVL0</accession>
    <name>A0A2K2CVL0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g06584v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CVL0"/>
    <dbReference type="EnsemblPlants" id="PNT66064">
        <property type="protein sequence ID" value="PNT66064"/>
        <property type="gene ID" value="BRADI_3g06584v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66065">
        <property type="protein sequence ID" value="PNT66065"/>
        <property type="gene ID" value="BRADI_3g06584v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66064">
        <property type="protein sequence ID" value="PNT66064"/>
        <property type="gene ID" value="BRADI_3g06584v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66065">
        <property type="protein sequence ID" value="PNT66065"/>
        <property type="gene ID" value="BRADI_3g06584v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CVL0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="126" mass="14041" checksum="26A9F0374EAAE78D" modified="2018-03-28" version="1">MESADPEPVAGQARGYGLVLVHRPATAYGPRRPGPHMRPLSSPRLLLSSTRSIPEIPIPSPEPPLLGSSCIPPSTCEPILLRPNPTPDKAIFQPRRKCRSRQWRRWGEGRSRSCPPERRRRRGVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CWK2</accession>
    <name>A0A2K2CWK2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC104583998</name>
        <name evidence="8" type="ORF">BRADI_3g11145v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236374.1">
        <property type="nucleotide sequence ID" value="XM_010238072.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CWK2"/>
    <dbReference type="STRING" id="15368.A0A2K2CWK2"/>
    <dbReference type="EnsemblPlants" id="PNT66408">
        <property type="protein sequence ID" value="PNT66408"/>
        <property type="gene ID" value="BRADI_3g11145v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583998"/>
    <dbReference type="Gramene" id="PNT66408">
        <property type="protein sequence ID" value="PNT66408"/>
        <property type="gene ID" value="BRADI_3g11145v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583998"/>
    <dbReference type="OrthoDB" id="608326at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044800">
        <property type="entry name" value="LEC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140:SF8">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS10G0537100-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR ABI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="7">
        <location>
            <begin position="108"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT66408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="275" mass="29738" checksum="8C4FAEFD2DBBD549" modified="2018-03-28" version="1">MENTQSLFGDTTQSEGDSVTTAKIKSSPPPTPSALAQPASRSAPDSSVIGAGASSEVDDGEDDPEVLMMEHLFDKVLTPGDMVDPSTNSYDRLTTVGPQRQQNPDDGLVFEDRAVAGKLWRFQSDWRSSEVHALCPTDGWALFAREKGLAPGDAVSFYRGNDNGRLFVDCSKRRGDRAPCPRGVFAWAPVAAATAAAAERPQVGADEAPRAAARRGTLPRRSPAVPRRPRRSKGKREEDDLDEAAMKPPTILESMPPLVPSPDPKRVRLFGIYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYA1</accession>
    <name>A0A2K2CYA1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g19507v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CYA1"/>
    <dbReference type="EnsemblPlants" id="PNT67008">
        <property type="protein sequence ID" value="PNT67008"/>
        <property type="gene ID" value="BRADI_3g19507v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67008">
        <property type="protein sequence ID" value="PNT67008"/>
        <property type="gene ID" value="BRADI_3g19507v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CYA1"/>
    <dbReference type="OrthoDB" id="645579at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CYA1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053197">
        <property type="entry name" value="F-box_SCFL_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223:SF81">
        <property type="entry name" value="OS08G0281600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223">
        <property type="entry name" value="OS11G0201299 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="27"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="465" mass="52457" checksum="3B2A87B847D1D124" modified="2018-03-28" version="1" fragment="single">MRRRTAAVRWVPRRRRAGGGVDRLSALPDALLHLIMSSLKAWEVVRTCVLSRRWRDLWASAPCVDIRVFYSGRDGAPPRELRGFVYRLFLLRDPSAPVHTLRLQSNDEDDSFCDGDADTWIKTAIKSNARVIRLDGRRGDFASLDHTAFVFSSHLKILKLSYVLDLKDCLVTGHEFSSASLKALTMSKCKIVVDFSIDAPNLVLLRLIIPYIRIPTFKNLGSLVTGTIVLVDSCLGDDFQGLSDADHSGETDDVDDDSDTSENYNMGYGHAYIGDYSYGTNGTYKYSEIISDCDDEKYGYHGDGHNCSKDGNYCDYGATANYGRNESKSLDVNSVLQSLRNARTLELLSYSGEVLLNRDFKSCPTFSNLKTMSLGEWCIAADFDALVLLLQHSPNLERLFLELILNLNIRKALERGLKLKERSFACKHLRMVEIKCSKDDVRVHMLAELFKANGIPHEKLYVHWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYM1</accession>
    <name>A0A2K2CYM1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100844691</name>
        <name evidence="16" type="ORF">BRADI_3g21300v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Two-heme-containing cytochrome. Catalyzes ascorbate-dependent trans-membrane electron transfer by utilizing a concerted H(+)/e(-) transfer mechanism.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573743.4">
        <property type="nucleotide sequence ID" value="XM_003573695.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CYM1"/>
    <dbReference type="STRING" id="15368.A0A2K2CYM1"/>
    <dbReference type="EnsemblPlants" id="PNT67123">
        <property type="protein sequence ID" value="PNT67123"/>
        <property type="gene ID" value="BRADI_3g21300v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844691"/>
    <dbReference type="Gramene" id="PNT67123">
        <property type="protein sequence ID" value="PNT67123"/>
        <property type="gene ID" value="BRADI_3g21300v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="907479at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CYM1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1770:FF:000001">
        <property type="entry name" value="Cytochrome b reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043205">
        <property type="entry name" value="CYB561/CYBRD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106">
        <property type="entry name" value="CYTOCHROME B561-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106:SF42">
        <property type="entry name" value="OS10G0118800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="15">
        <location>
            <begin position="71"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="PNT67123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="275" mass="29990" checksum="2F5559B510BE0325" modified="2018-03-28" version="1">MRLGSSTGTPPSSSYNRPFQSPRNNAEASTHFAVVIAVLLLPAPGRTNGEEAMPVKSGASFRASALPVIVMAQLLAAAVLTLTLVWVLHFRDGVSWELNSTTQLVYTVHPLFMVIGFIICTGEAIMAYRIVLGSRTAKKAVHLLLHLLALAFAAVGLYAAVKFHNDAGDPHVRSLHAWLGVVTIGLYALQWLVAFMYFVFPGAMMTMRADYAPWHIFFGVVIFLMAVCTTETGLVRFIYRIVGYPSEALVVNFTGLVILMFGVTVVLAVILPSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CZQ0</accession>
    <name>A0A2K2CZQ0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g28307v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CZQ0"/>
    <dbReference type="FunCoup" id="A0A2K2CZQ0">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67509">
        <property type="protein sequence ID" value="PNT67509"/>
        <property type="gene ID" value="BRADI_3g28307v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67509">
        <property type="protein sequence ID" value="PNT67509"/>
        <property type="gene ID" value="BRADI_3g28307v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CZQ0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34538">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34538:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT67509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="65" mass="7652" checksum="BAED34E95D04CCA3" modified="2018-03-28" version="1">MVSLAKISGSSGAWRAPAPVRQLYWRVRKAVLRRPRRDEARFGYDLQSYSRNFDDGQQLVFAYRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D1K8</accession>
    <name>A0A2K2D1K8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g36675v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Rokhsar D."/>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
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                <person name="Barry K."/>
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                <consortium name="EnsemblPlants"/>
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        <source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="2">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
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        <source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">Bd21</strain>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D8L0"/>
    <dbReference type="EnsemblPlants" id="PNT70618">
        <property type="protein sequence ID" value="PNT70618"/>
        <property type="gene ID" value="BRADI_2g14530v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70618">
        <property type="protein sequence ID" value="PNT70618"/>
        <property type="gene ID" value="BRADI_2g14530v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D8L0"/>
    <dbReference type="OrthoDB" id="1857192at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D8L0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000298">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000211">
        <property type="entry name" value="Dual-specific kinase DSK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF14">
        <property type="entry name" value="INACTIVE PROTEIN KINASE SELMODRAFT_444075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="394"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="177"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="681"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="215"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="697"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="716"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT70618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="749" mass="82623" checksum="3DA15989D0D59165" modified="2018-03-28" version="1">MAADGEQQSRGPEEDAAVVVVAARAAAREVPRTAVAWALTHVVQRGDSILLLVLMPPPPSSGRKFWGFPLFAGDCASGHKAVSNQKSDVSELCAQMMLKLRDVYDPSKINVKVRIVSGSPSGVVATESKRAHASWVVLDRELKLEEKRCMEELQCNIVVMKRSRPKVLRLNLVKSPERVKSEATAPPQPELSASVHENASDVKEEQISIRGPAVTPSSSPEPETPFYSTDVGTSSVSSSDPATSPFCASETNSSLKKEATMDITGQLDVNISDSESETSAPPAASSLQPWMADILEEPASSRLVRNRPRTRRTPTADALLEKISKLDLLTEISAVRSRSDLNFRGNVRDVVSLSRSAPPGPPPLCSICQHKTPVFGKPPRWFSYAELDHATGGFSKANFLAEGGFGSVHRGVLPDGQAIAVKQHKLASSQGDVEFCSEVEVLSCAQHRNVVMLIGFCVEDKRRLLVYEYICNRSLDTHLYGRSKETLGWAARQKIAVGAARGLRYLHEECRVGCIIHRDMRPNNILVTHDFEPLVGDFGLARWQPDGDMGVETRVIGTFGYLAPEYAQSGQITEKADVYSFGVVLVELVTGRKAVDINRPKGQQFLTEWARPLLEDHAIDELIDPRLEDRFCENEVYCMLHAANLCIRRDPHSRPRMSHVLRILEGDMVLESGCILAPSSDAGSMSRRMQSDRLHFQEQSSPVQPDPQSQCVGEVNRSHETLRSAWDPDREDLSHRLWYPSAADCSQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DJB7</accession>
    <name>A0A2K2DJB7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine dioxygenase</fullName>
            <ecNumber evidence="3">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_1g13412v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:20442"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DJB7"/>
    <dbReference type="EnsemblPlants" id="PNT74370">
        <property type="protein sequence ID" value="PNT74370"/>
        <property type="gene ID" value="BRADI_1g13412v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74370">
        <property type="protein sequence ID" value="PNT74370"/>
        <property type="gene ID" value="BRADI_1g13412v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DJB7"/>
    <dbReference type="OrthoDB" id="271433at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DJB7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012864">
        <property type="entry name" value="PCO/ADO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07847">
        <property type="entry name" value="PCO_ADO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT74370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="102" mass="11298" checksum="945860ADFF6972FF" modified="2018-03-28" version="1">MTAESEAWVLYPSTGGNVQRLAATADGPCALIQVLSPPRGSRLQHNIGYDFYKDMPCEQIHPAVLNQISDEQKGQLAWLQQTGVPKDLNLLTLRNNRDPVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DJU4</accession>
    <name>A0A2K2DJU4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g17503v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DJU4"/>
    <dbReference type="EnsemblPlants" id="PNT74549">
        <property type="protein sequence ID" value="PNT74549"/>
        <property type="gene ID" value="BRADI_1g17503v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74549">
        <property type="protein sequence ID" value="PNT74549"/>
        <property type="gene ID" value="BRADI_1g17503v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DJU4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT74549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="37" mass="4286" checksum="C898DE419E3463AA" modified="2018-03-28" version="1">MTDKSNCRQEIELMFGIRKRKGGQQVSAVKSFRRGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKN0</accession>
    <name>A0A2K2DKN0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g22825v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Rokhsar D."/>
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            <authorList>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="3" key="3">
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="1">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">Bd21</strain>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
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                <person name="Vogel J.P."/>
                <person name="Catalan P."/>
                <person name="Contreras-Moreira B."/>
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                <person name="Harmon-Smith M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="30S ribosomal protein S3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000008">
        <property type="entry name" value="30S ribosomal protein S3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_B">
        <property type="entry name" value="Ribosomal_uS3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005704">
        <property type="entry name" value="Ribosomal_uS3_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01009">
        <property type="entry name" value="rpsC_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF42">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 14" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 11 14" id="KW-0934">Plastid</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 9 10" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS3 C-terminal" evidence="13">
        <location>
            <begin position="152"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ACF08676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CZT22522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK18202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="VGO01924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="239" mass="27554" checksum="F65C62FFEEED49B2" modified="2008-09-02" version="1">MGQKINPLGFRLGTTQKHHSFWFAQPKNYSEGLQEDKKIRNCIKNYIQKNRKKGSNRKIESDSSSEVITHNRKMDSGSSSEVITHIEIQKEIDTIHVIIHIGFPNLLKKKGVIEELEKDLQKEINSVNQRLNISIEKVKEPYRQPNILAEYIAFQLKNRVSFRKAIKKAIELTKKADIRGVKVKIAGRLGGKEIARAECIKKGRLPLQTIRAKIDYCCYPIRTIYGVLGVKIWIFVDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPC5</accession>
    <name>I1GPC5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100825061</name>
        <name evidence="8" type="ORF">BRADI_1g11810v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560997.1">
        <property type="nucleotide sequence ID" value="XM_003560949.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPC5"/>
    <dbReference type="STRING" id="15368.I1GPC5"/>
    <dbReference type="EnsemblPlants" id="KQK13682">
        <property type="protein sequence ID" value="KQK13682"/>
        <property type="gene ID" value="BRADI_1g11810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825061"/>
    <dbReference type="Gramene" id="KQK13682">
        <property type="protein sequence ID" value="KQK13682"/>
        <property type="gene ID" value="BRADI_1g11810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825061"/>
    <dbReference type="eggNOG" id="ENOG502S0SZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076461_1_1_1"/>
    <dbReference type="OMA" id="RVSCEVY"/>
    <dbReference type="OrthoDB" id="1917236at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1820:FF:000005">
        <property type="entry name" value="Expressed protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="7">
        <location>
            <begin position="107"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK13682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="225" mass="24558" checksum="F40DECA5F51D848A" modified="2012-06-13" version="1">MAKTQPRASAGEPLLPSASPPPYLDHHSPVGAGAESYVYLVPVRLRRLRRGCRCRWLAPLLASLTLLALGFLLWPADPDVSVARLRLAHISVVARPAVALNISAALKVRVRNPDLFALDYSRLDIAIGYRGAPLGTVTSGGGRVRARAVSYIDADLHLNGIRVVEDAIYLLEDLARGSIPFDTVVEVEGHLHFFFISIPVKGRIACVVHVNPHDQTIVHQDCYPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPL5</accession>
    <name>I1GPL5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ThiC-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100843013</name>
        <name evidence="12" type="ORF">BRADI_1g12550v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561310.1">
        <property type="nucleotide sequence ID" value="XM_003561262.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPL5"/>
    <dbReference type="STRING" id="15368.I1GPL5"/>
    <dbReference type="EnsemblPlants" id="KQK13796">
        <property type="protein sequence ID" value="KQK13796"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74317">
        <property type="protein sequence ID" value="PNT74317"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74318">
        <property type="protein sequence ID" value="PNT74318"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74319">
        <property type="protein sequence ID" value="PNT74319"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74320">
        <property type="protein sequence ID" value="PNT74320"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74321">
        <property type="protein sequence ID" value="PNT74321"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74322">
        <property type="protein sequence ID" value="PNT74322"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74323">
        <property type="protein sequence ID" value="PNT74323"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74324">
        <property type="protein sequence ID" value="PNT74324"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843013"/>
    <dbReference type="Gramene" id="KQK13796">
        <property type="protein sequence ID" value="KQK13796"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74317">
        <property type="protein sequence ID" value="PNT74317"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74318">
        <property type="protein sequence ID" value="PNT74318"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74319">
        <property type="protein sequence ID" value="PNT74319"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74320">
        <property type="protein sequence ID" value="PNT74320"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74321">
        <property type="protein sequence ID" value="PNT74321"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74322">
        <property type="protein sequence ID" value="PNT74322"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74323">
        <property type="protein sequence ID" value="PNT74323"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74324">
        <property type="protein sequence ID" value="PNT74324"/>
        <property type="gene ID" value="BRADI_1g12550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843013"/>
    <dbReference type="eggNOG" id="ENOG502QRQ4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_1_1"/>
    <dbReference type="OMA" id="FDWNKQF"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.540:FF:000001">
        <property type="entry name" value="Phosphomethylpyrimidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="entry name" value="Radical SAM ThiC family, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006763">
        <property type="entry name" value="PRK09284.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009895">
        <property type="entry name" value="PRK13352.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557:SF1">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="THIAMINE BIOSYNTHESIS PROTEIN THIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01114">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00113">
        <property type="entry name" value="Radical_SAM_Phosphomethylpyrim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="42"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK13796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="643" mass="71904" checksum="5A4111467CA2BCDB" modified="2012-06-13" version="1">MAALQPSFSSTMAMNNSCSTMKFPKTALLPCFGGISRPQDVQDRNAGFNSSRPKAVSLADQSIQEPAKPRQKKQTVDPTAPEFLPLPSFEECFPRSTKECSEIVHEESGHVLKVPFRRVHLTGDNEHFDTYDTSGPQNISPSLGLPKIRKEWIDRREKLASPRYTQMYYAKQGIITEEMVYCAKRENLTPEFVRSEVARGRAIIPSNKRHLELEPMIVGRNFLVKVNANIGNSAVVSSIEEEVHKLQWATMWGADTVMDLSTGRHIHETREWIIRNSSVPIGTVPIYQALEKVNGIAEDLSWEVFRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLTYHKENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDSAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMEKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKRHPYAQAWDDALSKARFEFRWLDQFALSLDPVTAMSFHDETLPSDGAKVAHFCSMCGPKFCSMKITEDIRKYADEHGYGTVEEAVRQGMSDMSAEFLAARKTISGEQHGEAGGEIYVPESYVATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRU2</accession>
    <name>I1GRU2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100843330</name>
        <name evidence="9" type="ORF">BRADI_1g19830v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May play a role in vesicular transport from endoplasmic reticulum to Golgi.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Golgi apparatus</location>
            <location evidence="1 8">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562577.1">
        <property type="nucleotide sequence ID" value="XM_003562529.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRU2"/>
    <dbReference type="FunCoup" id="I1GRU2">
        <property type="interactions" value="3303"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GRU2"/>
    <dbReference type="EnsemblPlants" id="KQK14966">
        <property type="protein sequence ID" value="KQK14966"/>
        <property type="gene ID" value="BRADI_1g19830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843330"/>
    <dbReference type="Gramene" id="KQK14966">
        <property type="protein sequence ID" value="KQK14966"/>
        <property type="gene ID" value="BRADI_1g19830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843330"/>
    <dbReference type="eggNOG" id="KOG3330">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087110_0_1_1"/>
    <dbReference type="OMA" id="MVQMQVQ"/>
    <dbReference type="OrthoDB" id="10262857at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033106">
        <property type="term" value="C:cis-Golgi network membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14942">
        <property type="entry name" value="TRAPPC3_bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000006">
        <property type="entry name" value="Trafficking protein particle complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016721">
        <property type="entry name" value="Bet3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13048">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018293">
        <property type="entry name" value="TRAPP_I_complex_Bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK14966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="187" mass="21012" checksum="136913D4CFEE57D6" modified="2012-06-13" version="1">MPPLTPPKSGDALFSSVERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGTRLVDEFLAKSNVSRCVDFKETADTIAKLGFKMFLGVTATVTNWDAEGTTCSFVLEDNPLVDFVELPDTCQGLQYCNVLSGVIRGALEMVSMKTEVTWVRDMLRGDDAYEMRVKLTKQVPEEYPYKDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1GVQ1</accession>
    <name>I1GVQ1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4-coumarate--CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100842528</name>
        <name evidence="13" type="ORF">BRADI_1g31320v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(E)-4-coumarate + ATP + CoA = (E)-4-coumaroyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19641"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19642"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(E)-4-coumarate + ATP + H(+) = (E)-4-coumaroyl-AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:72419"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:72420"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(E)-4-coumaroyl-AMP + CoA = (E)-4-coumaroyl-CoA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:72423"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:72424"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.12" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563338.1">
        <property type="nucleotide sequence ID" value="XM_003563290.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GVQ1"/>
    <dbReference type="STRING" id="15368.I1GVQ1"/>
    <dbReference type="EnsemblPlants" id="KQK16898">
        <property type="protein sequence ID" value="KQK16898"/>
        <property type="gene ID" value="BRADI_1g31320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842528"/>
    <dbReference type="Gramene" id="KQK16898">
        <property type="protein sequence ID" value="KQK16898"/>
        <property type="gene ID" value="BRADI_1g31320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842528"/>
    <dbReference type="eggNOG" id="KOG1176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_2_1"/>
    <dbReference type="OMA" id="RVAAYKY"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GVQ1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016207">
        <property type="term" value="F:4-coumarate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106290">
        <property type="term" value="F:trans-cinnamate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010044">
        <property type="term" value="P:response to aluminum ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd05904">
        <property type="entry name" value="4CL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF149">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="11">
        <location>
            <begin position="47"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="12">
        <location>
            <begin position="473"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK16898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="572" mass="60992" checksum="C86C3E83A515366A" modified="2012-06-13" version="1">MGSVAAEEFPAPVPTPAAAETVFRSKLPDIEIPAHLTLQAYCFERLAEVSARPCLIDAQSGAVHTYGEVDALSRRAAAGLRRRLGVGKGDVVMLLLRNCAEFAFVFLGAARLGAAATTANPFCTPHEIHRQASAAGAKVIVTEPCAAEKVRAFAAERGVAVVVVVEGDGAAPDGCLEFKDAILGEDCGAGEEEEEEEAVVDPDDVVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGENPNLYFRKEDVVLCLLPLFHIYSLNSVLLAGLRAGCAIVVMRKFDHGALAAAVRAHGVTVAPFVPPIVVEITKSDRVTAGDLASIRMVMSGAAPMGKDLQDSFMAKLPNAVLGQGYGMTEAGPVLAMCLAFAKEPFEVKSGSCGTVVRNAELKIVDPDTGASLGRNQPGEICIRGQQIMKGYLNDPEATKNTIDKDSWLHTGDIGYVDDDEEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMQDELAGEVPVAFVVRTQGSEISENDIKQFVAKEVVFYKRIHKVFFADSIPKSPSGKILRKDLRAKLAAGIPSDDNTQSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZJ8</accession>
    <name>I1GZJ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100834390</name>
        <name evidence="8" type="ORF">BRADI_1g45190v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003564002.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZJ8"/>
    <dbReference type="EnsemblPlants" id="KQK18869">
        <property type="protein sequence ID" value="KQK18869"/>
        <property type="gene ID" value="BRADI_1g45190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834390"/>
    <dbReference type="Gramene" id="KQK18869">
        <property type="protein sequence ID" value="KQK18869"/>
        <property type="gene ID" value="BRADI_1g45190v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834390"/>
    <dbReference type="eggNOG" id="KOG1303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009646_1_1_1"/>
    <dbReference type="OMA" id="ICTHWYQ"/>
    <dbReference type="OrthoDB" id="655540at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GZJ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF698">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="11"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK18869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="399" mass="42859" checksum="7C8A60F288080F7C" modified="2012-06-13" version="1">MDTSAPSKSGTGFFKTCFNGVNALSGVGILSIPYALSQGGWLSLLIFTTIAIICFYTGILLQRCINSSSLVKTYPDIGELAFGRKGKIIVAIFMYLELYLVAIDFMILEGDNLDKLFPSVNFHVAGLKIGGKQGFVLIFSLLVLPTTWFRSLNALAYVSLGGIFASIVLIASVTWVGAFDGVGFHEKGVLLNWAGIPTAMSLYSFCFSGHAVFPMIYTGMSDRKKFPTVLLSCFIVCTLGYGLMGVVGYLMFGKSLRSQVTLNLPTRNWSSSIAIYTTLINPFTKFALLVTPIAEAIEDSLHVGKNKAVSVTIRTALVVSTTIVALSVPFFAYAVALTGSFLSGTATMLLPCACYLKIRSRTCRKLGFEQVVCLGIIVVGVGVVVVGTCSSLKQIVQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1H0S2</accession>
    <name>I1H0S2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100824888</name>
        <name evidence="5" type="ORF">BRADI_1g48530v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. PrmA family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564278.1">
        <property type="nucleotide sequence ID" value="XM_003564230.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H0S2"/>
    <dbReference type="FunCoup" id="I1H0S2">
        <property type="interactions" value="535"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H0S2"/>
    <dbReference type="EnsemblPlants" id="KQK19482">
        <property type="protein sequence ID" value="KQK19482"/>
        <property type="gene ID" value="BRADI_1g48530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824888"/>
    <dbReference type="Gramene" id="KQK19482">
        <property type="protein sequence ID" value="KQK19482"/>
        <property type="gene ID" value="BRADI_1g48530v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824888"/>
    <dbReference type="eggNOG" id="KOG3420">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074702_1_1_1"/>
    <dbReference type="OMA" id="DVVYSIH"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H0S2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008988">
        <property type="term" value="F:rRNA (adenine-N6-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="4">
        <location>
            <begin position="48"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00041374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK19482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="222" mass="24534" checksum="3CD2D9C1E18534CD" modified="2012-06-13" version="1">MKLKQLEGLLGGLTQFSNPKVELEQYATGPHIASRMLYTAENSFGDITGKVVADFGCGCGTLAVASALLDAEHVTGIDIDLQSLELAQENADDLELDIDLIQCDIKNLNLKGLLVDTVVMNPPFGTKRNGADMEFLSMGLKAASQAVYSLHKTSTREYIKKAALRNCNALSAEVLCELRYDLPQTYEFHKKKEVDVAVDLWRFVPRARDESTAERHGNKMRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1H3Q2</accession>
    <name>I1H3Q2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100843195</name>
        <name evidence="3" type="ORF">BRADI_1g57360v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557594.1">
        <property type="nucleotide sequence ID" value="XM_003557546.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H3Q2"/>
    <dbReference type="FunCoup" id="I1H3Q2">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H3Q2"/>
    <dbReference type="EnsemblPlants" id="KQK20894">
        <property type="protein sequence ID" value="KQK20894"/>
        <property type="gene ID" value="BRADI_1g57360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843195"/>
    <dbReference type="Gramene" id="KQK20894">
        <property type="protein sequence ID" value="KQK20894"/>
        <property type="gene ID" value="BRADI_1g57360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843195"/>
    <dbReference type="eggNOG" id="KOG2852">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_14_2_1"/>
    <dbReference type="OMA" id="DDTVYAC"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000360">
        <property type="entry name" value="FAD-dependent oxidoreductase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF150">
        <property type="entry name" value="OXIDOREDUCTASE TDA3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="12"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK20894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="386" mass="40105" checksum="9ABBC60B54C95D0B" modified="2012-06-13" version="1">MATAPAAAPPRRVVVCGGGVVGACTAYFLSTHAASPTVPTLIEKSSPACAASGKAGGFLALDWSDSTPSLSALARASFALHGRLAAALDGADAYGFRPVHTLSVSLPSHPISPSSPPHPLLPPWVDPSASAAPPRELGTPATTAQVHPGLFTKAVLAASGAEVVIGEVERVVLGPDDRVAGVQVKGRGVVEADAVVLALGPWSGRFEMVREVFDVSGLKAHSIVLRPRDPDKITPHCLFLSYQPEPGAKMLDPEVYPRPTGEVYICGMTKDEDVPDDPATIVGEPDSIAMLHKIAGRVSSQLKTEEGAEVVAEQACYLPCTNDGLPVIGEMPGVKGCYVATGHSCWGILNAPATGAALAELILDGQAKTVDLTLFSPARFLKKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H556</accession>
    <name>I1H556_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 22">Peroxidase</fullName>
            <ecNumber evidence="5 22">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC100841701</name>
        <name evidence="24" type="ORF">BRADI_1g61540v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19 22">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="19 22">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19 22">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="19 22">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 22">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="5 22"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228581.1">
        <property type="nucleotide sequence ID" value="XM_010230279.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H556"/>
    <dbReference type="FunCoup" id="I1H556">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H556"/>
    <dbReference type="EnsemblPlants" id="KQK21559">
        <property type="protein sequence ID" value="KQK21559"/>
        <property type="gene ID" value="BRADI_1g61540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841701"/>
    <dbReference type="Gramene" id="KQK21559">
        <property type="protein sequence ID" value="KQK21559"/>
        <property type="gene ID" value="BRADI_1g61540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841701"/>
    <dbReference type="eggNOG" id="ENOG502QPX7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010543_0_1_1"/>
    <dbReference type="OMA" id="CHASPYY"/>
    <dbReference type="OrthoDB" id="600458at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 22" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 22" id="KW-0349">Heme</keyword>
    <keyword evidence="17 22" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="13 19 22" id="KW-0408">Iron</keyword>
    <keyword evidence="9 19 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 22" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="16" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 22" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982621" description="Peroxidase" evidence="22">
        <location>
            <begin position="23"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="23">
        <location>
            <begin position="27"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="20">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="37"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="70"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="124"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQK21559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="330" mass="35031" checksum="4800A0175AD93996" modified="2012-06-13" version="1">MAAKVAVLVACALLLLAVGCQASPYWPLEVGFYHDKCPQAEAVVKGVVANAIAQNPGNGAALIRMLFHDCFVEGCDASILLDATPFSPTPEKTSPPNDPTLRGFELIDAIKDAVEAACPGVVSCADILAFAARDASCVLSGGKADFTMPGGRRDGTYSNASEPLKFLVPPTSTLAELVDSFVVKGLNTEDLVILSGAHTVGRSHCSSFVPDRLLSPASDIGSGFAAFLRGQCPADATAGGNDAVVMQDVVTPDALDRQYYKNVLSHTVLFSSDAALLTSEETVRMVMDNANIPGWWEDRFKTSMVKMASIEVKTGFQGQIRKNCRAINYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6S9</accession>
    <name>I1H6S9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter G family member 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">White-brown complex homolog protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100844207</name>
        <name evidence="14" type="ORF">BRADI_1g66120v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential transporter for growth and development under abiotic stress. Mediates shoot branching by promoting the outgrowth of lateral shoots. Required for salt tolerance via Na/K homeostasis, at least partly by regulating SKC1/OsHKT1;5. Necessary for hypodermal suberization of roots, which contributes to formation of the apoplastic barrier.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="KQK22254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003558150.4"/>
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    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK22254">
        <property type="protein sequence ID" value="KQK22254"/>
        <property type="gene ID" value="BRADI_1g66120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844207"/>
    <dbReference type="Gramene" id="KQK22254">
        <property type="protein sequence ID" value="KQK22254"/>
        <property type="gene ID" value="BRADI_1g66120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844207"/>
    <dbReference type="eggNOG" id="KOG0061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_57_8_1"/>
    <dbReference type="OMA" id="AGQMCTG"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:ABC-type transporter activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="535"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="612"/>
            <end position="633"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="640"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="761"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="123"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="26"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
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            <begin position="75"/>
            <end position="108"/>
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    </feature>
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        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK22254"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="788" mass="86990" checksum="BAA7B63F20E3FD55" modified="2012-06-13" version="1">MSRFVDKLPFFDRRASPMEEADDIPRSGLLHLHGGHHHYHHQQPAAQSAMMSPQPSPPTKQSSFTLAQLLKRVNEARSDVSSPNSSPSHYTIELGGSAPGSTGSDGSELTAHAGVGTLLPFVLKFTDLTYSVKQRKKGPCLPALPFRAAAEPEAPRMKTLLDNISGEAREGEIMAVLGASGSGKSTLIDALANRIRKESLHGSVTLNGESMDNNLLKVISAYVMQDDLLYPMLTVEETLMFSAEFRLPRSLPTKEKKKRVQALIDQLGLRNAANTIIGDEGHRGVSGGERRRVSIGVDIIHDPIVLFLDEPTSGLDSTSAFMVVKVLQRIAQSGSVVVMSIHQPSYRILGLLDRLLFLSRGQTVYYGPPGALSSFFHDFGKPIHDNENPTEFALDLVRELETMPDGARDLVEHNKSWQKRMGPKMKHHDDDGEKPSLSLKEAISASISRGKLVSGATDGNVTVSSSASPESAVSKFANPFWVEMGVLTRRAFLNTKRTPEIFVIRLGAVLITGFILATIFWRLDDSPKGVEERLGFFAIAMSTMFYTCSDALPVFLNERYIFLRETAYNAYRRSSYVLSHTIVGFPSLIVLSLAFAVTTFFAVGLAGGVDGFFFFVAIVLASFWAGSGFATFLSGVVTNVMLGFPVVVSTLAYFLLFSGFFINRDRIPKYWLWFHYASLVKYPYEAVMINEFSDPSRCFVRGVQMFDNTPLSILSPAVKVRVLRAMSSSLGINIGTSTCITTGPDFLKQQAVNDLTKWECLWITVAWGFLFRILFYIALLLGSRNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1H728</accession>
    <name>I1H728_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100834464</name>
        <name evidence="8" type="ORF">BRADI_1g66950v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK22394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003561739.4"/>
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    <dbReference type="STRING" id="15368.I1H728"/>
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        <property type="protein sequence ID" value="KQK22394"/>
        <property type="gene ID" value="BRADI_1g66950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834464"/>
    <dbReference type="Gramene" id="KQK22394">
        <property type="protein sequence ID" value="KQK22394"/>
        <property type="gene ID" value="BRADI_1g66950v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834464"/>
    <dbReference type="eggNOG" id="KOG0595">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14009">
        <property type="entry name" value="STKc_ATG1_ULK_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Serine/threonine-protein kinase ATG1a"/>
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    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Atg1-like"/>
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        <property type="entry name" value="MIT_ATG1a/b/c"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF68">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATG1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24497">
        <property type="entry name" value="MIT_ATG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="13"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="278"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="367"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="461"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="461"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK22394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="704" mass="77949" checksum="520DB9B15E744891" modified="2012-06-13" version="1">MEDHGGGRRVGEYMLVRPIGSGAYSQVWLGKHLVRGTEVAVKEIAMERLSSKLRDSLLSEVDILRRIRHPNIIALHDSIKDSGRIYLILEYCRGGDLYSYLMRHKRVPETVAKHFIRQLACGLQMLRDNNVVHRDLKPQNILLVANNENSILKIADFGFAKFLQPSCLAETLCGSPLYMAPEVMQAQKYDAKADLWSVGIILYQLVTGSPPFNGDNQIQLLKNILKSGQLRFPSDCELSHECIDLCRKLLRISSVERLTVEEFVNHPFLFEHAPERTLSRTPSDTRDGFPFIKSSPTRLSSQSSQEDCMPFPLDLSTGQDEGAVPESSSPLKSYGFATNKQLDKTSGQSPSKHTGLFSKYIMGNNYAHSNQRMDHPGKRTKESKIGVGRDPKGGYPEDSPIIDSLEFVDQEYVFVPGNPEGSSSSTSASLQRNLPLKYDYSSVSPPDLAALSAPMPINGTAINRQQSAGTGSLDSHCSPVSGTSQGSAYLSDGMDQPPSDYLTRIRLLGRYASAIVELVKREMKDGRHLEAFSIQLIVLATWKQAIHICNFYAASASRESPSHDIIMKGLDADAPHLLANSQLANDECMQIERQFLIDVECAEELASTVGQIPDATAMPDAVEIIFQSALELGRHGGVDEMMGKVALAISQYTKAVCMLRFLLTEAPSLALNPPLSLARSDRHRLRSYIEALNTRLGQLQCQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1H764</accession>
    <name>I1H764_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100822162</name>
        <name evidence="8" type="ORF">BRADI_1g67260v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558292.1">
        <property type="nucleotide sequence ID" value="XM_003558244.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H764"/>
    <dbReference type="FunCoup" id="I1H764">
        <property type="interactions" value="2505"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H764"/>
    <dbReference type="EnsemblPlants" id="KQK22444">
        <property type="protein sequence ID" value="KQK22444"/>
        <property type="gene ID" value="BRADI_1g67260v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822162"/>
    <dbReference type="Gramene" id="KQK22444">
        <property type="protein sequence ID" value="KQK22444"/>
        <property type="gene ID" value="BRADI_1g67260v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822162"/>
    <dbReference type="eggNOG" id="KOG1607">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028277_5_0_1"/>
    <dbReference type="OMA" id="ESMWKFA"/>
    <dbReference type="OrthoDB" id="537032at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H764">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="term" value="P:response to molecule of fungal origin"/>
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        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
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        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF0">
        <property type="entry name" value="LD18904P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005225">
        <property type="entry name" value="LAG1_LAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="60"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK22444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="284" mass="33142" checksum="ECF60C7FC7A9EAC4" modified="2012-06-13" version="1">MAIRGPDAASFFPLTLLFSLGFFCARFLLDRLVYKPLAVYLFTSKGSKLMNDEARQAKIVKFSESTWKLTYYASVQAWVLLIIKQEPWSLDTMQYFDGWPNQPIPSLLTLFYMCQCGFYIYSIFALIAWETRRKDFAVMMSHHVVTSVLIGYSYLTGFFRIGTIILALHDASDVFLETAKLCKYTEKELGASLFFGLFALSWLLLRLIYFPFWIIKTSSYQSIISLRKLDRFPTTLYYIFNTMLLTLLVFHMYWGKLIFLMIMRQLNNKGKVGEDVRSDSEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1H8X5</accession>
    <name>I1H8X5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC104581853</name>
        <name evidence="7" type="ORF">BRADI_1g72330v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229077.1">
        <property type="nucleotide sequence ID" value="XM_010230775.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H8X5"/>
    <dbReference type="STRING" id="15368.I1H8X5"/>
    <dbReference type="EnsemblPlants" id="KQK23265">
        <property type="protein sequence ID" value="KQK23265"/>
        <property type="gene ID" value="BRADI_1g72330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581853"/>
    <dbReference type="Gramene" id="KQK23265">
        <property type="protein sequence ID" value="KQK23265"/>
        <property type="gene ID" value="BRADI_1g72330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104581853"/>
    <dbReference type="eggNOG" id="ENOG502QRAZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058918_1_0_1"/>
    <dbReference type="OMA" id="IASAACC"/>
    <dbReference type="OrthoDB" id="1909330at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044837">
        <property type="entry name" value="REM16-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391:SF4">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS03G0184500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS11G0197600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="135"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK23265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="235" mass="26602" checksum="74FB031108F899DF" modified="2012-06-13" version="1">MAIEKGISPYEAARERKVQENKRKMEALNLHHLSAAVAHAPKTPSPMKQKRRRIIQAAVVAPSPPRRSRRLAHLPEVKYAEVAPESERMKRWSPRKPTEIMCWGSGGSISMEARLEAARKAEELESELDPEFPSFVKRMLHSHVVRGFWLGLPSHFCDSYLPKQDSTITLVDEKDEEFETNYLAYKKGLSGGWAGFALSHGILDGDSTVFQLIKPTTFKVHIIRATVDNDNKESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HBB7</accession>
    <name>I1HBB7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100838889</name>
        <name evidence="1" type="ORF">BRADI_2g01090v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566511.1">
        <property type="nucleotide sequence ID" value="XM_003566463.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HBB7"/>
    <dbReference type="FunCoup" id="I1HBB7">
        <property type="interactions" value="1256"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HBB7"/>
    <dbReference type="EnsemblPlants" id="KQK02375">
        <property type="protein sequence ID" value="KQK02375"/>
        <property type="gene ID" value="BRADI_2g01090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838889"/>
    <dbReference type="Gramene" id="KQK02375">
        <property type="protein sequence ID" value="KQK02375"/>
        <property type="gene ID" value="BRADI_2g01090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838889"/>
    <dbReference type="eggNOG" id="ENOG502QW45">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058925_0_0_1"/>
    <dbReference type="OMA" id="FLEAHMC"/>
    <dbReference type="OrthoDB" id="783877at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37181">
        <property type="entry name" value="F6A14.6 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37181:SF1">
        <property type="entry name" value="F6A14.6 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK02375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="378" mass="39571" checksum="7DB09C762C96AA8D" modified="2012-06-13" version="1">MTLLAAITNPTPESVAAKGHPLVLTPGAPPPSAKFSALPAPILPETWSLAPADPTLATAASFLAASLLGAPLSVQRFRTLVTSFLATLSESLSLPRPAAAALQEAIRAAAPYFPATLAPLVASAAAGLAEHDVLLALADARALPHPPPGLLAALSDAGRPDLVCAVLRQAADLRSSELLAALRCFLSPASDEAYDAMVGVKGRWKDAAVLAVNKCVDKGAGKKAKPTARRAAMLLMMGHDGFTSPEVCLHYLFASRNVDCVESVVLAAAVAELDGVEVAGLMRYLAKWIGKYRRFPEAQACPEAAGMLGLDQCESVPSFGAMARAMGLVLDQHFSHLVLNAEVRDELRAAEEMVKELAAEAESSGPILDLLRRMQHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCB7</accession>
    <name>I1HCB7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g04090v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCB7"/>
    <dbReference type="FunCoup" id="I1HCB7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HCB7"/>
    <dbReference type="EnsemblPlants" id="KQK02850">
        <property type="protein sequence ID" value="KQK02850"/>
        <property type="gene ID" value="BRADI_2g04090v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02850">
        <property type="protein sequence ID" value="KQK02850"/>
        <property type="gene ID" value="BRADI_2g04090v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S1GQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094776_1_0_1"/>
    <dbReference type="InParanoid" id="I1HCB7"/>
    <dbReference type="OMA" id="KGCRVAM"/>
    <dbReference type="OrthoDB" id="691043at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33257:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33257">
        <property type="entry name" value="OS05G0165500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK02850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="238" mass="24880" checksum="23995664C55C5584" modified="2012-06-13" version="1">MAATGVRQEEPRQKGAKFVLSRLLSRDSTATGARAPSFRVYYGVASAGAVPFLWESQPGTPKSSPADHAPLPVPPLTPPPSYYQLAGAKEDAARRNRKAGSKTKRKSSRPAAAAGPTLASFLSLSLSLPRTGTGTGKPRSASSSSSSSSSSSSFSSLSLSFRGARSPAQGSSMRRGSSRMHAFSDEEEEEDAAAAMTTCFRVRQESFRALRGCRAAMAVRSALASVGGTATAHTHRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCW7</accession>
    <name>I1HCW7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pollen-specific protein SF21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100845470</name>
        <name evidence="3" type="ORF">BRADI_2g05770v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315186.1">
        <property type="nucleotide sequence ID" value="XM_024459418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCW7"/>
    <dbReference type="STRING" id="15368.I1HCW7"/>
    <dbReference type="ESTHER" id="bradi-i1hcw7">
        <property type="family name" value="Ndr_family"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK03135">
        <property type="protein sequence ID" value="KQK03135"/>
        <property type="gene ID" value="BRADI_2g05770v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845470"/>
    <dbReference type="Gramene" id="KQK03135">
        <property type="protein sequence ID" value="KQK03135"/>
        <property type="gene ID" value="BRADI_2g05770v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2931">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035361_2_0_1"/>
    <dbReference type="OMA" id="KTCFQPL"/>
    <dbReference type="OrthoDB" id="741027at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HCW7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK03135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="38612" checksum="58999F5E206B2783" modified="2012-06-13" version="1">MGDSSGSVSVDVERIYFGGKAHPVRTRYGSVSVSVYGDEDKPALITYPDVALNYMSCFQGLFFCPEAASLLLHNFCIYHITPQGHELGAAPISSDVPVLSVDDLADQVADVLDFFGLGSVMCLGVTAGAYVLTLFATKYRERVLGLMLVSPLCKAPSWTEWLYNKVLLNLLYYCGTRGLVNECLLQRYFSAEVRGDGQDPESEIVQACRSLLDERKGVNVFRFLKSVNERRDLTEALKKLQCRTLIFVGENSQFHDDAVHMTTKLDRRYCALVEVQACGSLVTEEQPHAMLIPMEYFLIGYGLYRPSQLDSSPRSTLNPFCISPELLSPESMGVKLKPIKTRISLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HDE6</accession>
    <accession>A0A0Q3ISG4</accession>
    <name>I1HDE6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_2g07320v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK03356.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HDE6"/>
    <dbReference type="FunCoup" id="I1HDE6">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK03356">
        <property type="protein sequence ID" value="KQK03356"/>
        <property type="gene ID" value="BRADI_2g07320v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03356">
        <property type="protein sequence ID" value="KQK03356"/>
        <property type="gene ID" value="BRADI_2g07320v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSMM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015101_2_1_1"/>
    <dbReference type="InParanoid" id="I1HDE6"/>
    <dbReference type="OMA" id="VIPVGCA"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF620">
        <property type="entry name" value="OS01G0223000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003642614" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK03356.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="40133" checksum="E69203A755CCE8DE" modified="2012-06-13" version="1">MRDFRCAVSLLLLLVANLGLGRAAAPGALSVRIRPYDSIFSFGDSYADTGNNPVVFAANSIFDPVTRPPYGSAFFGGRPTGRNSNGRLIIDFVAQGLGLPLLPPSLAHNGSFRRGANFAVGGATALDAAFFHSQSKFPLNTSLGVQLEWFDSLKPSICRTTQECEEFFGRSLFFVGEFGINDYHFSISVKSLQEIMSFVPDVVGTISKAIETLMNHGVRSFVVPGMIPSGCAPPVLAMFAHADPSKYNSTTGCLEDYNKLGMHHNLLLQEALEKLRKRHPDATIIYADLFGPIMEMVESPSKFGFEEDVLNICCGGPGTLWCGDEGAKLCEKPSARLFWDGVHLTEAAYGYIANGWLDNIAPLQDRAVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1HIU8</accession>
    <name>I1HIU8_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g23540v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HIU8"/>
    <dbReference type="FunCoup" id="I1HIU8">
        <property type="interactions" value="1063"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK05951">
        <property type="protein sequence ID" value="KQK05951"/>
        <property type="gene ID" value="BRADI_2g23540v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK05952">
        <property type="protein sequence ID" value="KQK05952"/>
        <property type="gene ID" value="BRADI_2g23540v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05951">
        <property type="protein sequence ID" value="KQK05951"/>
        <property type="gene ID" value="BRADI_2g23540v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05952">
        <property type="protein sequence ID" value="KQK05952"/>
        <property type="gene ID" value="BRADI_2g23540v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S1FS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107362_2_0_1"/>
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    <dbReference type="OMA" id="YYGAREF"/>
    <dbReference type="OrthoDB" id="1733797at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33738">
        <property type="entry name" value="EMB|CAB82975.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33738:SF8">
        <property type="entry name" value="OS05G0454500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK05951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="146" mass="15662" checksum="DE37332837B3E131" modified="2012-06-13" version="1">MDDKKKSPPAPAAAAAATAPAPANGYFSSVFSASPAANPRDARETDLNTILNRQNPKGPTSGGIADGKFQGSPTKGRVTYKDGQQFYPNEPSESPYFGSSVHYGGRDFYDSSPHKQASESSRSYKDDNPDGSLATRGDWWQGSLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQ93</accession>
    <name>I1HQ93_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100830820</name>
        <name evidence="4" type="ORF">BRADI_2g46220v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314826.1">
        <property type="nucleotide sequence ID" value="XM_024459058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQ93"/>
    <dbReference type="EnsemblPlants" id="KQK09133">
        <property type="protein sequence ID" value="KQK09133"/>
        <property type="gene ID" value="BRADI_2g46220v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72558">
        <property type="protein sequence ID" value="PNT72558"/>
        <property type="gene ID" value="BRADI_2g46220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830820"/>
    <dbReference type="Gramene" id="KQK09133">
        <property type="protein sequence ID" value="KQK09133"/>
        <property type="gene ID" value="BRADI_2g46220v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72558">
        <property type="protein sequence ID" value="PNT72558"/>
        <property type="gene ID" value="BRADI_2g46220v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_49_0_1"/>
    <dbReference type="OMA" id="KWPKQIT"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HQ93">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051240">
        <property type="entry name" value="Mito_RNA-Proc/Resp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933:SF25">
        <property type="entry name" value="OS01G0672166 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="193"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="228"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="263"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="298"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK09133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="52007" checksum="19E2739E2D8DFC5C" modified="2012-06-13" version="1">MPPANLTPAKWPKTLTAGHLHRLVRAERDPRRALALFDAATAASVSAPILPSPDTVSLLTSRLASAGLLPLATSLLSRSRALFPSAADLEPPFLTLLRAFSRTHRPLAALQLFRSAPSALALPHSARSYTAVLATLVSHSHLPLAHSLLADMRAGGFAPTTATYNVLLKAHCSDASAPIEDAVRLFRNIPKPDACSYNTVIDGLCRRSRRADAQEIFSEMVANGVAPTVVTYTTVINWLARDGCLDDALKMFDEMGRIGIAPNVVTYSSLIDGLCKGGRAASALELLDRMAKEAKLPNTITYSSVINGLCKEDRLNEAMEVLDRMRLQGRKPDAGLFGKLIVGLCGAGRAVEAANYLDEMVLSGVEPNRVTWSLHVRINDAVVTALCAKGEAGRTFRVYQSMRTRGISTEPGTFHLLVEFFSKKNDLEKAAHVVLDMLSEKCIPERETWDIILTRYWSKKKVRQEAEQMWNQLTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1HRZ7</accession>
    <name>I1HRZ7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100835721</name>
        <name evidence="3" type="ORF">BRADI_2g50960v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569896.1">
        <property type="nucleotide sequence ID" value="XM_003569848.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HRZ7"/>
    <dbReference type="FunCoup" id="I1HRZ7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09922">
        <property type="protein sequence ID" value="KQK09922"/>
        <property type="gene ID" value="BRADI_2g50960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835721"/>
    <dbReference type="Gramene" id="KQK09922">
        <property type="protein sequence ID" value="KQK09922"/>
        <property type="gene ID" value="BRADI_2g50960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835721"/>
    <dbReference type="eggNOG" id="ENOG502QQ1N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043615_1_0_1"/>
    <dbReference type="OMA" id="DLAAMSW"/>
    <dbReference type="OrthoDB" id="2021158at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31923">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31923:SF22">
        <property type="entry name" value="OS01G0766600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="2">
        <location>
            <begin position="189"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK09922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="410" mass="44891" checksum="DA8340F1CC231D42" modified="2012-06-13" version="1">MSWLARSIAATLSSAPSDDDDSVETSGEKSPDHAAHPPNADDEKDEQPDTPGRGVKGDISELTESLSRRFWGVASFLAPPPQAEAEAVETVAADGESSGAQSPRVAGIRSDLAEIGGRVRSGISILSNANAMAEISKIASSFLPFVEDEEEEQVEAVGVTEEVVVFVRHISTQPETWRDFPLFVNDRQTDDFELSDTQCEHALAIERIVPSLSYLRTELCSTNMSEACFWKIYFVLLHSKLNMQDAELLSTTQILEAREELLQSSPKMKKLGSEGPGGPSGSRNVSSTQAEDSEFLPSSIQCKSEIFEAVSFQEPIPDPLRGIETVKHPILTSEPEMVDKSVIEEMVVTNEIKSILAVKPSLHTAEDDDDKEVEEWLEDMEHVSSKTGNVTLACQDEDVSFSDLEDEDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSN1</accession>
    <name>I1HSN1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100822726</name>
        <name evidence="7" type="ORF">BRADI_2g52890v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754759.1">
        <property type="nucleotide sequence ID" value="XM_014899273.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSN1"/>
    <dbReference type="FunCoup" id="I1HSN1">
        <property type="interactions" value="1977"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HSN1"/>
    <dbReference type="EnsemblPlants" id="KQK10242">
        <property type="protein sequence ID" value="KQK10242"/>
        <property type="gene ID" value="BRADI_2g52890v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822726"/>
    <dbReference type="Gramene" id="KQK10242">
        <property type="protein sequence ID" value="KQK10242"/>
        <property type="gene ID" value="BRADI_2g52890v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822726"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="IQFTHAN"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HSN1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094785" evidence="4">
        <location>
            <begin position="27"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="5">
        <location>
            <begin position="25"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="72"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK10242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="214" mass="22873" checksum="351BEE0DBE757EDF" modified="2012-06-13" version="1">MAAAAAGALGLFLAAALVLALAGANSEGDALSALRRSLQDPGGVLQSWDPTLVNPCTWFHVTCNRENRVTRLDLGNLNLSGHLVPELGKLDHLQYLELYKNNIQGTIPSELGDLKNLISLDLYKNNVSGTIPPTLGKLKSLVFLRLNGNRLTGPIPRELAGISSLKVVDVSGNDLCGTIPTTGPFEHIPLSNFEKNPRLEGPELQGLAVYDTNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZ72</accession>
    <name>I1HZ72_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g09310v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZ72"/>
    <dbReference type="FunCoup" id="I1HZ72">
        <property type="interactions" value="493"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HZ72"/>
    <dbReference type="EnsemblPlants" id="KQJ94225">
        <property type="protein sequence ID" value="KQJ94225"/>
        <property type="gene ID" value="BRADI_3g09310v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ94225">
        <property type="protein sequence ID" value="KQJ94225"/>
        <property type="gene ID" value="BRADI_3g09310v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583490"/>
    <dbReference type="eggNOG" id="ENOG502R64K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127920_0_0_1"/>
    <dbReference type="OMA" id="VSLMGHE"/>
    <dbReference type="OrthoDB" id="691231at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ94225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="185" mass="20202" checksum="30537A62B51581AA" modified="2012-06-13" version="1">MQTDQVRGQDIGEGMNRLAPQEYYYHAGLDRPAATGGLSEADGVLVLKELLEEEDEDEAEKYSTSSPPHVDDDDADRLSRVMRSLEAEIMRGGDNGECLARPPGPGEINGCVQLDQMLLDFDDGFGGAAGFGYYWPEVPAGGIYVYSEAGEGSAVGYEMQVAREQQYYCADQCSGEQVYSSSLWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1I418</accession>
    <name>I1I418_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100826987</name>
        <name evidence="14" type="ORF">BRADI_3g26880v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. May replace conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573931.1">
        <property type="nucleotide sequence ID" value="XM_003573883.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I418"/>
    <dbReference type="STRING" id="15368.I1I418"/>
    <dbReference type="EnsemblPlants" id="KQJ96738">
        <property type="protein sequence ID" value="KQJ96738"/>
        <property type="gene ID" value="BRADI_3g26880v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ96739">
        <property type="protein sequence ID" value="KQJ96739"/>
        <property type="gene ID" value="BRADI_3g26880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826987"/>
    <dbReference type="Gramene" id="KQJ96738">
        <property type="protein sequence ID" value="KQJ96738"/>
        <property type="gene ID" value="BRADI_3g26880v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96739">
        <property type="protein sequence ID" value="KQJ96739"/>
        <property type="gene ID" value="BRADI_3g26880v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826987"/>
    <dbReference type="eggNOG" id="KOG1757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062828_2_2_1"/>
    <dbReference type="OMA" id="MNKKGAP"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000005">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="13">
        <location>
            <begin position="109"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ96739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="139" mass="14542" checksum="E91624D04647346D" modified="2012-06-13" version="1">MAGKGGKGLLAAKTTAAKGAAAADKDKKKAPVSRSSRAGIQFPVGRIHRQLKQRVSANGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDTLIKGTIAGGGVIPHIHKSLINKTSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5H8</accession>
    <name>I1I5H8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100836884</name>
        <name evidence="2" type="ORF">BRADI_3g31440v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574215.1">
        <property type="nucleotide sequence ID" value="XM_003574167.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5H8"/>
    <dbReference type="EnsemblPlants" id="KQJ97498">
        <property type="protein sequence ID" value="KQJ97498"/>
        <property type="gene ID" value="BRADI_3g31440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836884"/>
    <dbReference type="Gramene" id="KQJ97498">
        <property type="protein sequence ID" value="KQJ97498"/>
        <property type="gene ID" value="BRADI_3g31440v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836884"/>
    <dbReference type="eggNOG" id="ENOG502QS8H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_49_3_1"/>
    <dbReference type="OMA" id="MDQRWLP"/>
    <dbReference type="OrthoDB" id="294702at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763:SF68">
        <property type="entry name" value="HYDROLASE, ALPHA_BETA FOLD FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="77"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ97498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="351" mass="38581" checksum="AFE12969650EE7A1" modified="2012-06-13" version="1">MDRSVEGAAAQYYSSAMGPLAGQLGEWLCRAVQPPAPTPCGSPGGPPVTARRVRLRDGRHLAYEETGVPLHRARFRVVFSHGFTGSRLDSLRASPEVAEELGVYMVGFDRAGYGESDPNPARTVESAALDVADLADALGLGDKFHLVGFSLGCHAVWGALRYIPHRLAGAAMLAPVVNYWWPGFPPELAAQEYGKQARGDQWALRVSHHAPGLLHWWMEQSWLPLPTSTVASNTTHLPNKRDAEVRRTLAADGTLRAKREMATQQGIMESYYRDMAVMFGKWEFDPMALPEPECEVHLWQGDEDGLVPVVLQRHVAGSLRWVKYHELPGTGHFLSAVPGLGDTVLRTLFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5L3</accession>
    <name>I1I5L3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="8">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100842160</name>
        <name evidence="10" type="ORF">BRADI_3g31767v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Extracellular space</location>
            <location evidence="8">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="8"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574233.1">
        <property type="nucleotide sequence ID" value="XM_003574185.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5L3"/>
    <dbReference type="STRING" id="15368.I1I5L3"/>
    <dbReference type="EnsemblPlants" id="KQJ97550">
        <property type="protein sequence ID" value="KQJ97550"/>
        <property type="gene ID" value="BRADI_3g31767v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842160"/>
    <dbReference type="Gramene" id="KQJ97550">
        <property type="protein sequence ID" value="KQJ97550"/>
        <property type="gene ID" value="BRADI_3g31767v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842160"/>
    <dbReference type="eggNOG" id="ENOG502QS5E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048041_0_0_1"/>
    <dbReference type="OMA" id="LPYTFHT"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02176">
        <property type="entry name" value="GH16_XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008263">
        <property type="entry name" value="GH16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01034">
        <property type="entry name" value="GH16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0052">Apoplast</keyword>
    <keyword evidence="8" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="1 8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982779" description="Xyloglucan endotransglucosylase/hydrolase" evidence="8">
        <location>
            <begin position="26"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="9">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="7">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ97550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="289" mass="32316" checksum="80B3D9616B73A24E" modified="2012-06-13" version="1">MERMTKALLAAVAMAAVLELGLVGANFLDKCDITWEPQNAKMTEGGNHLTLSLVSNSSGSMLRTKKQFIFGSVSTMMKLVKGNSAGTVTTYYTSSVDNDHDEIDFEFLGNETGQPYTIHTNVFADGVGAKEMQFRPWFDPTADFHNYTIFWNPSMIVWLVDRIPIRVFRNYAKEGVAYPTKRPMYGFSSIWAAEDWATQGGRVKTDWSKAPFVAEYREISLQVCECASAACAETCASSRYAAPGTHKLTDKQLRELKAVQLGYTIYDYCKDAKWPNGTQVPLECGMDQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6E4</accession>
    <name>I1I6E4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100845812</name>
        <name evidence="5" type="ORF">BRADI_3g34110v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235030.2">
        <property type="nucleotide sequence ID" value="XM_010236728.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6E4"/>
    <dbReference type="STRING" id="15368.I1I6E4"/>
    <dbReference type="EnsemblPlants" id="KQJ97919">
        <property type="protein sequence ID" value="KQJ97919"/>
        <property type="gene ID" value="BRADI_3g34110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845812"/>
    <dbReference type="Gramene" id="KQJ97919">
        <property type="protein sequence ID" value="KQJ97919"/>
        <property type="gene ID" value="BRADI_3g34110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845812"/>
    <dbReference type="eggNOG" id="KOG4209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_23_4_1"/>
    <dbReference type="OMA" id="PGRTHPW"/>
    <dbReference type="OrthoDB" id="591723at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I6E4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12306">
        <property type="entry name" value="RRM_II_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF106">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="92"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ97919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="204" mass="23053" checksum="EDFB89E76F09661A" modified="2012-06-13" version="1">MDSNGNSDDRSLNPDAGDIVLGIGSSRSRIIDQEIEEMQRKLRELGEEIDLAIVSLNEQIEMTTDQISGVSAEEEEDPAVAIARHKSEVDSRSIYVGNVDYACLPEQIQAHFQDCGAINRVTILIDDFGDPKGYAYVEFAEVEAVEKALLMNDTKLLNRPMKVSPKRTNVPGRTHPWGRRPFQPYPTYGKFPRFRRPQGYSPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1I725</accession>
    <name>I1I725_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100823484</name>
        <name evidence="2" type="ORF">BRADI_3g35900v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572192.1">
        <property type="nucleotide sequence ID" value="XM_003572144.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I725"/>
    <dbReference type="STRING" id="15368.I1I725"/>
    <dbReference type="EnsemblPlants" id="KQJ98281">
        <property type="protein sequence ID" value="KQJ98281"/>
        <property type="gene ID" value="BRADI_3g35900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823484"/>
    <dbReference type="Gramene" id="KQJ98281">
        <property type="protein sequence ID" value="KQJ98281"/>
        <property type="gene ID" value="BRADI_3g35900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823484"/>
    <dbReference type="OrthoDB" id="2014563at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I725">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021788">
        <property type="entry name" value="CPP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33372">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33372:SF2">
        <property type="entry name" value="PROTEIN CHAPERONE-LIKE PROTEIN OF POR1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11833">
        <property type="entry name" value="CPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ98281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="265" mass="29262" checksum="4D9B837F2C20EFCF" modified="2012-06-13" version="1">MQAAVAVSQTSFLARPLRRLPRPYHADGAGDGASRWGGASLTQLRRARCFASLSVGAGNYGSEHGPVFPILKTWDPYKLLGVDHDASEEEINSARNFLLQQYAGYEESEEAIEGAYDKIMMKSYSLRKRSKINLKSKLRKQVEESPSWVKTLLGYFEVPSMDVLSRRLALFAFIAGWSIATSAETGPTFQLALSLASCIYFLNDKMKNLARASAMGFGVFVCGWIAGSLLVPVIPSFVVPLTWSLELLTSLVVYAFLFFGCTFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1I7X1</accession>
    <name>I1I7X1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Impact N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100839841</name>
        <name evidence="3" type="ORF">BRADI_3g38380v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317960.1">
        <property type="nucleotide sequence ID" value="XM_024462192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I7X1"/>
    <dbReference type="FunCoup" id="I1I7X1">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I7X1"/>
    <dbReference type="EnsemblPlants" id="KQJ98674">
        <property type="protein sequence ID" value="KQJ98674"/>
        <property type="gene ID" value="BRADI_3g38380v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839841"/>
    <dbReference type="Gramene" id="KQJ98674">
        <property type="protein sequence ID" value="KQJ98674"/>
        <property type="gene ID" value="BRADI_3g38380v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3299">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="AIKSNCR"/>
    <dbReference type="OrthoDB" id="10262814at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I7X1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF20">
        <property type="entry name" value="IMPACT FAMILY MEMBER YIGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Impact N-terminal" evidence="2">
        <location>
            <begin position="65"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ98674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="245" mass="26522" checksum="611DAEA5CD28F4BB" modified="2012-06-13" version="1">MAAVRVVTPRLRSIPLLLPGPVHHSCSCRSSAASSVRDMSSSSASSSPSPYTALVGRVRCEREIKRSKFIAIAGTVHDERAAMSFLDEVKDPRATHNCWAYKVGEQFRYNDDGEPSSTAGKPIYSAIISSGIDMVMVVVIRYFGGIKLGTGGLVRAYGGVASECLKDAPTCLVKPKALVGMEVPFDLLGTVYHQLQHFQAEDIKQDYDTGKDGAVVVMFKVEYEKIESLGSAVNSACSRKIELLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IAF9</accession>
    <name>I1IAF9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100834525</name>
        <name evidence="13" type="ORF">BRADI_3g45590v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235528.1">
        <property type="nucleotide sequence ID" value="XM_010237226.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IAF9"/>
    <dbReference type="FunCoup" id="I1IAF9">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IAF9"/>
    <dbReference type="EnsemblPlants" id="KQJ99850">
        <property type="protein sequence ID" value="KQJ99850"/>
        <property type="gene ID" value="BRADI_3g45590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834525"/>
    <dbReference type="Gramene" id="KQJ99850">
        <property type="protein sequence ID" value="KQJ99850"/>
        <property type="gene ID" value="BRADI_3g45590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834525"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040248_0_0_1"/>
    <dbReference type="OMA" id="QYICHYD"/>
    <dbReference type="OrthoDB" id="3437960at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IAF9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000071">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000104">
        <property type="entry name" value="Transcriptional repressor protein YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000818">
        <property type="entry name" value="zinc finger transcription factor YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001190">
        <property type="entry name" value="zinc finger transcription factor YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF1">
        <property type="entry name" value="ZINC FINGER TRANSCRIPTION FACTOR YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="93"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="123"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="153"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="217"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="244"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ99850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="346" mass="39009" checksum="98758F75FEE8B50D" modified="2012-06-13" version="1">MQAAAPRLRHRWVKEWIPQDLVIAGGPCALYKWVREDRLSALKAKDKEQGADTAKPEPTTEVLFLCSYDGCGKTFVDAGALRKHAHVHGERQYICHYDGCGKKFLDSSKLKRHFLIHTGEKNFFCTYEGCGKAFSLDFNLKAHMKTHFADNYHPCPYPECGRRFTQESKLRAHFRTQHEKNPGMPAAMNRNALGDHPHNTVKPPPVTPPVPSAERPYVCPYDGCAKAYIHEYKLNLHLKKEHPNHFSDAGAQAGPSRSTASKSSRRSRPSATAKMPLPKIPKRGAEYTTPSPAINIAEEHQWPRKVLYDDDSEETEEEGDKPADMGWGRIAASSDDDDDEETEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IB42</accession>
    <name>I1IB42_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100825026</name>
        <name evidence="8" type="ORF">BRADI_3g47480v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575263.1">
        <property type="nucleotide sequence ID" value="XM_003575215.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IB42"/>
    <dbReference type="STRING" id="15368.I1IB42"/>
    <dbReference type="EnsemblPlants" id="KQK00130">
        <property type="protein sequence ID" value="KQK00130"/>
        <property type="gene ID" value="BRADI_3g47480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825026"/>
    <dbReference type="Gramene" id="KQK00130">
        <property type="protein sequence ID" value="KQK00130"/>
        <property type="gene ID" value="BRADI_3g47480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825026"/>
    <dbReference type="eggNOG" id="KOG1021">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012659_4_1_1"/>
    <dbReference type="OMA" id="SKTSWMF"/>
    <dbReference type="OrthoDB" id="589084at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF74">
        <property type="entry name" value="OS12G0224400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="76"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK00130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="495" mass="55840" checksum="352598C14E6FE3B8" modified="2012-06-13" version="1">MEAHYHNKCLLYALLLVGSWICSFLLHLQFFHLSLLRYSFPYYHRLRAASVPLEATFASSHAFAQDFTSSRRSPSCDGRYVYMLDIHPRFDLLNACVDGSSSLLEDEHGACVLMSNAGLGPALAPAASGDDGVISSNGWFNTNQYSLEVIFHNRMRHYECLTDDPASSSAVYVPYYPGLELNRHACEANATERDGPSGEFLRWLSSRPEWAAHGGRDHFMVVAKTTWMLRRRVQPDEEAGSCGNRFLDRAEPRNMTVLTYESNIWDRRDMAVPYPSYFHPSSSGAVSAWQARARAAPRPWLFAFAGARRPNGTLLLRDRVIDTCVSVPARCGMFGCDSQRGGLEGCRSPEKLVALFLSARFCLQPRGDSFMRRSSVDAVIAGCVPVFFHEASTFEKQYRWHAPQGNKSGGNYSVFIDPDDVLQGKVDIEEVLGRYTDEEVAAMREEVIRMIPRLLYKDPRVRFQGHMSDAFDIAIDEVLARTRRIKNGEDLGWQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1IB68</accession>
    <name>I1IB68_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Elongation factor Tu</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100830133</name>
        <name evidence="12" type="ORF">BRADI_3g47690v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575279.1">
        <property type="nucleotide sequence ID" value="XM_003575231.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IB68"/>
    <dbReference type="STRING" id="15368.I1IB68"/>
    <dbReference type="EnsemblPlants" id="KQK00163">
        <property type="protein sequence ID" value="KQK00163"/>
        <property type="gene ID" value="BRADI_3g47690v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68963">
        <property type="protein sequence ID" value="PNT68963"/>
        <property type="gene ID" value="BRADI_3g47690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830133"/>
    <dbReference type="Gramene" id="KQK00163">
        <property type="protein sequence ID" value="KQK00163"/>
        <property type="gene ID" value="BRADI_3g47690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68963">
        <property type="protein sequence ID" value="PNT68963"/>
        <property type="gene ID" value="BRADI_3g47690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830133"/>
    <dbReference type="eggNOG" id="KOG0460">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_0_1_1"/>
    <dbReference type="OMA" id="KTHANIG"/>
    <dbReference type="OrthoDB" id="2067at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03707">
        <property type="entry name" value="EFTU_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000001">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000046">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000003">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000766">
        <property type="entry name" value="PRK00049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009372">
        <property type="entry name" value="PRK12735.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009373">
        <property type="entry name" value="PRK12736.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF5">
        <property type="entry name" value="ELONGATION FACTOR TU, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="72"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT68963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="471" mass="50499" checksum="5FDF906B24671201" modified="2012-06-13" version="1">MASLAAASASTALLFPPSTSSSKPRLPLSTSLGFSTPARSRRAAAGAGAGSSGRRPGLLVVRAARAKFERTKPHVNIGTIGHVDHGKTTLTAALTMVLASVGGSAPKKYDEIDAAPEERARGITINTATVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPSIVVFLNKKDQVDDEELLELVDLEVRELLTAYEYNGDDVPIISGSALKALEALMATPGIKRGENEWVDGIFTLVDAVDAHIPVPQRQTDLPFLLAVEDVFSITGRGTVATGRIERGTVKVGDPVDLVGIRETRNATVTGVEMFQKTMDDAIAGDNVGLLLRGMQKEDIERGMVLAKPGSITPHTKFEAVVYVLKKEEGGRHSPFFPGYRPQFYMRTTDVTGNVTNIMNDKDEEAKMCMPGDRIKMVVELIQPVACEQGMRFAIREGGKTVGAGVINNIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEN1</accession>
    <name>I1IEN1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADPH-protochlorophyllide oxidoreductase</fullName>
            <ecNumber evidence="9">1.3.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100821409</name>
        <name evidence="10" type="ORF">BRADI_3g57380v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>chlorophyllide a + NADP(+) = protochlorophyllide a + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11132"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:83348"/>
            <dbReference type="ChEBI" id="CHEBI:83350"/>
            <dbReference type="EC" id="1.3.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Plastid</location>
            <location evidence="9">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.33" evidence="9"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570527.1">
        <property type="nucleotide sequence ID" value="XM_003570479.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEN1"/>
    <dbReference type="FunCoup" id="I1IEN1">
        <property type="interactions" value="662"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IEN1"/>
    <dbReference type="EnsemblPlants" id="KQK01659">
        <property type="protein sequence ID" value="KQK01659"/>
        <property type="gene ID" value="BRADI_3g57380v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821409"/>
    <dbReference type="Gramene" id="KQK01659">
        <property type="protein sequence ID" value="KQK01659"/>
        <property type="gene ID" value="BRADI_3g57380v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821409"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_3_1"/>
    <dbReference type="OMA" id="WSWGNRQ"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="UniPathway" id="UPA00668"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016630">
        <property type="term" value="F:protochlorophyllide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09810">
        <property type="entry name" value="LPOR_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005979">
        <property type="entry name" value="Prochl_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01289">
        <property type="entry name" value="LPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44419">
        <property type="entry name" value="PROTOCHLOROPHYLLIDE REDUCTASE C, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44419:SF20">
        <property type="entry name" value="PROTOCHLOROPHYLLIDE REDUCTASE C, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="9" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 9" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 9" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK01659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="394" mass="41905" checksum="C7585AB2EA0550DD" modified="2012-06-13" version="1">MALQAAASFLLPSSALSARKEGAVKDSAFLGVRLVDGLRLETGSLGLRTKRLNTSSVAIRAQATAVSEAPAAPAGKKAVRTGTAIITGASSGLGLATAKALSESGKWHVIMACRDYLKAARAARAAGMAKGSYTIVHLDLASLDSVRQFVSNVRGLGMPIDVVVCNAAVYQPTAKQPSFTADGFELSVGVNHLGHFLLARELLEDLKSSDFPSKRLIIVGSITGNTNTLAGNVPPKANLGDLRGLAAGLNGTASSAMIDGGEFDGAKAYKDSKVCNMLTMQEFHRRFHEETGVTFASLYPGCIATTGLFREHVPLFRFLFPPFQKYITKGYVSEEEAGKRLAQVVSEPSLTKSGVYWSWNQNSASFENQLSEEASDTEKARKVWELSEKLVGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1IF85</accession>
    <name>I1IF85_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100829808</name>
        <name evidence="5" type="ORF">BRADI_3g59200v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236113.1">
        <property type="nucleotide sequence ID" value="XM_010237811.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IF85"/>
    <dbReference type="STRING" id="15368.I1IF85"/>
    <dbReference type="EnsemblPlants" id="KQK01914">
        <property type="protein sequence ID" value="KQK01914"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01915">
        <property type="protein sequence ID" value="KQK01915"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01916">
        <property type="protein sequence ID" value="KQK01916"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01917">
        <property type="protein sequence ID" value="KQK01917"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01918">
        <property type="protein sequence ID" value="KQK01918"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69632">
        <property type="protein sequence ID" value="PNT69632"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829808"/>
    <dbReference type="Gramene" id="KQK01914">
        <property type="protein sequence ID" value="KQK01914"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01915">
        <property type="protein sequence ID" value="KQK01915"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01916">
        <property type="protein sequence ID" value="KQK01916"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01917">
        <property type="protein sequence ID" value="KQK01917"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01918">
        <property type="protein sequence ID" value="KQK01918"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69632">
        <property type="protein sequence ID" value="PNT69632"/>
        <property type="gene ID" value="BRADI_3g59200v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829808"/>
    <dbReference type="HOGENOM" id="CLU_011417_1_0_1"/>
    <dbReference type="OrthoDB" id="6363363at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IF85">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000476">
        <property type="entry name" value="Activating molecule in BECN1-regulated autophagy protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052596">
        <property type="entry name" value="AMBRA1_autophagy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22874">
        <property type="entry name" value="ACTIVATING MOLECULE IN BECN1-REGULATED AUTOPHAGY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22874:SF1">
        <property type="entry name" value="ACTIVATING MOLECULE IN BECN1-REGULATED AUTOPHAGY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="147"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="389"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="395"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK01916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="799" mass="86814" checksum="137D66C96F118C9F" modified="2012-06-13" version="1">MTEMAENWIEGSKHTSAPASFSGSVPRYQTSSPRKSRQRNIFHLLSHREVSPRTKHQAKRHWSKPPTGDTGFTELRYSASDAKHDLFSWAESQSLHRWSAKYCPLQPPPRSTIAAAFSSDGKTLASTHGDHTVKIIDCQTGKCLKVLGGHRRTPWVVRYHPLHSDILASGSLDYEVRLWDANTSDCIVSHDFHRPIASIAFHALGDILAVASGHKLFIWKYNNREEVSAPSIILRTRRSLRAVHFHPHGAPYLLTAEVNNLDAADSQLTRATSTGYANYPPALFFANVNSRGYPHLESSMSSPCLIWPAFLRDDGSLYIPRGDLASSSTNVQQSSPSLAQNLLASDAENQQSDQFVTPMDICPGEPSASNDIVDNSSAAGLSAIQMHADRGQTDSRLQGSSSSSSLERSSARDDVPMASTSTSVPIPAIARPSGPAVNRLPTNSFTTSSGLDVQMFLRNSEGGNHHHDLFSDSRSWELPFLHGWLMAQNHTGVPSSLPIATGSTRGSNRRYDSRPHAFSSVPGVGSSLLGPQIDEAEAHVASLGVGSELTTSLFAAGAAELPCTVKLRVWRHDIKNPCSALETAACCLTISHAVLCSEMGAHFSPCGRFLVACVACMLPQTEGDQGSQLPVQYDSTGPGTSPTRHPLPSHGVIYELRVYSLEEATFGEILTSRAIRAAHCLTSIQFSPTSEHILLAYGRRHSSLLRSIIMDGETTGIPVYTILEVYRVSDMELIRVLPSAEDEVNVACFHPSPGGGLVYGTKEGKLRILQHNGADAASLGLNCFIEENMLEVQRYALEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPK3</accession>
    <name>I1IPK3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">isochorismate synthase</fullName>
            <ecNumber evidence="5">5.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100833004</name>
        <name evidence="13" type="ORF">BRADI_4g28670v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate = isochorismate</text>
            <dbReference type="Rhea" id="RHEA:18985"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29780"/>
            <dbReference type="EC" id="5.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isochorismate synthase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.4.2" evidence="5"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238089.1">
        <property type="nucleotide sequence ID" value="XM_010239787.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPK3"/>
    <dbReference type="SMR" id="I1IPK3"/>
    <dbReference type="FunCoup" id="I1IPK3">
        <property type="interactions" value="497"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IPK3"/>
    <dbReference type="EnsemblPlants" id="KQJ89950">
        <property type="protein sequence ID" value="KQJ89950"/>
        <property type="gene ID" value="BRADI_4g28670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833004"/>
    <dbReference type="Gramene" id="KQJ89950">
        <property type="protein sequence ID" value="KQJ89950"/>
        <property type="gene ID" value="BRADI_4g28670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833004"/>
    <dbReference type="eggNOG" id="KOG1223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_8_3_1"/>
    <dbReference type="OMA" id="CLQPPDA"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IPK3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008909">
        <property type="term" value="F:isochorismate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042372">
        <property type="term" value="P:phylloquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.120.10:FF:000005">
        <property type="entry name" value="isochorismate synthase, chloroplastic-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004561">
        <property type="entry name" value="IsoChor_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044250">
        <property type="entry name" value="MenF-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00543">
        <property type="entry name" value="isochor_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47253">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47253:SF4">
        <property type="entry name" value="ISOCHORISMATE SYNTHASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="12">
        <location>
            <begin position="279"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ89950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="560" mass="61741" checksum="BABB1403AA7E0AAD" modified="2012-06-13" version="1">MAPPSSLYSSGRLLPSRPASMARLVGRWVRPSCALSMNGCASGTGTGDAVSIRETRALPAASAPQEAVGQLRAAVAALHTDPPASSSGIIRIEVPIRQRGDAIEWLHAQSSLPRCFFSARAPLPDRPPPFNGNGNGLIKAEQSWQQPVSVAGVGSAVFFRGTDPFSLRDWRAIKRFLSRDCPLIRAYGAIRFDATSDTSVEWEDYGSFYFIVPQVEFSELEESSVLATTIAWDDSLSWTWQNAVNELQSTLQKISPCSVKVNKSSLQTTIINLNHVPTKASWDVAVTKALQMIKRRQRELVKVVLARCSRYITDTCIDPLELLACLKVEGQNAYQFCIQPPDAPAFVGNSPEQLFHRKYLDISSEALAGTRARGKTRADDFQIGQDLLLSIKEDTEFTIVRDSIKKKLEMICDEVVVHPSKALRKLPRVQHLSAQLAARLRSEDDEFDILNALHPSPAVCGLPTEEARQFIRDYEIFDRGMYAGPVGWFGGAESEFAVGIRSALLGKGHSTLVYAGAGIVEGTNPSFEWDELDLKASQFAKLLQYQEQHICYQEVGNMGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IU17</accession>
    <name>I1IU17_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha,alpha-trehalose-phosphate synthase (UDP-forming)</fullName>
            <ecNumber evidence="3">2.4.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Trehalose 6-phosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100824241</name>
        <name evidence="9" type="ORF">BRADI_4g41580v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-trehalose 6-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="2.4.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578837.1">
        <property type="nucleotide sequence ID" value="XM_003578789.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IU17"/>
    <dbReference type="STRING" id="15368.I1IU17"/>
    <dbReference type="EnsemblPlants" id="KQJ92080">
        <property type="protein sequence ID" value="KQJ92080"/>
        <property type="gene ID" value="BRADI_4g41580v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT65386">
        <property type="protein sequence ID" value="PNT65386"/>
        <property type="gene ID" value="BRADI_4g41580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824241"/>
    <dbReference type="Gramene" id="KQJ92080">
        <property type="protein sequence ID" value="KQJ92080"/>
        <property type="gene ID" value="BRADI_4g41580v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65386">
        <property type="protein sequence ID" value="PNT65386"/>
        <property type="gene ID" value="BRADI_4g41580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824241"/>
    <dbReference type="eggNOG" id="KOG1050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_3_1_1"/>
    <dbReference type="OMA" id="FEHARHF"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000010">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000052">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000054">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000017">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000002">
        <property type="entry name" value="Trehalose-6-phosphate synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF120">
        <property type="entry name" value="TREHALOSE 6-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT65386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="877" mass="98583" checksum="374A7D8981AFB19B" modified="2012-06-13" version="1">MFSRSYTNLLDLANGNLSALDYGGGGGGGGGGGSGGGRPPRARRMQRTMTTPGTLAELDEERAGSVASDAQSSLASDRIIVVANTLPVRCERRPDGRGWSFSWDEDSLLLHLRDGLPEDMEVLYVGSLRADVPAAEQDDVAQALLDRFRCVPAFLPKDLSDRFYHGFCKQTLWPLFHYMLPFTSDHGGRFDRSNWEQYVLANKLFSQRVIEVLNPEDDYIWIHDYHLLALPSFLRRRFNRLRIGFFLHSPFPSSELYRSLPVRDEILKSLLNCDLIGFHTFDYARHFLSCCSRMLGIEYQSKRGYIGLDYFGRTVGIKIMPVGINMPQLKSQLQLPDLEWRVAELRKQFAGKTVLLGVDDMDIFKGINLKILAFEQMLKIHPKWQGRAVLVQIANPRSGSGKDLEGLKAEIEESCARINGQFGRPGYSPVELVNRAISSVERMAYYTVAECVVVTAVRDGMNLTPYEYIVCRQGIPGLDGSSGDAPKRKSMLVVSEFIGCSPSLSGAIRVNPWNIDTTAEAMNESIALADNEKQLRHEKHYRYVSTHDVAYWSKSYVHDLERSCRDHFRRRCWGIGLGFGFRVVALDRNFKKLTVDSIVADYKKSNSRVILLDYDGTLVPQTTINRTPNETVVNIMNSLCADKKNVVFIVSGRGRDSLAKWFNSCPELGIAAEHGYFMRWTRDEQWKINNQCSEFGWMQMAEPVMNLYTEATDGSYIETKESALVWHHQDADPGFGSAQAKEMLDHLESVLANEPVSVKSGQHIVEVKPQGVNKGFVAEKILSMLTENKRQADFVLCIGDDRSDEDMFEGIADIMKRSIVDPQTLLYACTVGQKPSKAKYYLDDTNDVLNMLEALADASEEVGSPEESKILSPSEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>I1IV15</accession>
    <name>I1IV15_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100828546</name>
        <name evidence="16" type="ORF">BRADI_4g44460v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579023.3">
        <property type="nucleotide sequence ID" value="XM_003578975.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318439.1">
        <property type="nucleotide sequence ID" value="XM_024462671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IV15"/>
    <dbReference type="STRING" id="15368.I1IV15"/>
    <dbReference type="EnsemblPlants" id="KQJ92559">
        <property type="protein sequence ID" value="KQJ92559"/>
        <property type="gene ID" value="BRADI_4g44460v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92560">
        <property type="protein sequence ID" value="KQJ92560"/>
        <property type="gene ID" value="BRADI_4g44460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828546"/>
    <dbReference type="Gramene" id="KQJ92559">
        <property type="protein sequence ID" value="KQJ92559"/>
        <property type="gene ID" value="BRADI_4g44460v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92560">
        <property type="protein sequence ID" value="KQJ92560"/>
        <property type="gene ID" value="BRADI_4g44460v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_59_0_1"/>
    <dbReference type="OMA" id="LMFATAW"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IV15">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000279">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000096">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF87">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="12"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="NAF" evidence="15">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQJ92559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="444" mass="50039" checksum="E0B0A0FF2B5F2386" modified="2012-06-13" version="1">MDSSGKVVLGRYEVGRLLGKGAFGKVHYAKNLKSNRSVAIKMLDKEKVLKVGLSEQVRREITTMRLVAHKNIVKLHEVMATRSKIYFVMEYVKGGQLFDKISNGGKLTESAAHKYFQQLISAVDYCHSRGVYHRDLKPENLLLDEHENLKVSDFGLSALSESKRQDGLLHTICGSPAYVAPEVISKGGYDGAKSDIWSCGVVLFVLVAGYLPFQGQNLMEMYMKIEQGDFKCPGWVSIKLRKLLHKIMDPDPSKRISIQKIKESTWFRKGPEETLTMERIPIENAPTNAAPTLMRRRKNSHGDAKPLAVTNLNAFEIISFSTGFDLSGLFVEKESKKETRFTSENPASAIISKLEDVAKALNLRVRKKDNGVVKMQGRKEGRNGVVQFDSEIFQITPSYHLIEMKQTSGDSLEYQKLLEDGIRPALKDIVWAWHGDDDHHQQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1IWU7</accession>
    <name>I1IWU7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">PSII reaction center subunit V</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BRADI_5g06936v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IWU7"/>
    <dbReference type="STRING" id="15368.I1IWU7"/>
    <dbReference type="EnsemblPlants" id="KQJ82159">
        <property type="protein sequence ID" value="KQJ82159"/>
        <property type="gene ID" value="BRADI_5g06936v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82159">
        <property type="protein sequence ID" value="KQJ82159"/>
        <property type="gene ID" value="BRADI_5g06936v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S2SP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194095_0_0_1"/>
    <dbReference type="InParanoid" id="I1IWU7"/>
    <dbReference type="OrthoDB" id="1839964at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009579">
        <property type="term" value="C:thylakoid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009767">
        <property type="term" value="P:photosynthetic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.860">
        <property type="entry name" value="Photosystem II cytochrome b559, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006217">
        <property type="entry name" value="PSII_cyt_b559_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037025">
        <property type="entry name" value="PSII_cyt_b559_asu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013081">
        <property type="entry name" value="PSII_cyt_b559_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013082">
        <property type="entry name" value="PSII_cytb559_asu_lum"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01332">
        <property type="entry name" value="cyt_b559_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33391">
        <property type="entry name" value="CYTOCHROME B559 SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33391:SF21">
        <property type="entry name" value="PSII REACTION CENTER SUBUNIT V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00283">
        <property type="entry name" value="Cytochrom_B559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00284">
        <property type="entry name" value="Cytochrom_B559a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000036">
        <property type="entry name" value="PsbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161045">
        <property type="entry name" value="Cytochrome b559 subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="11" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Photosystem II cytochrome b559 N-terminal" evidence="13">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Photosystem II cytochrome b559 alpha subunit lumenal region" evidence="14">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQJ82159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9349" checksum="99C18232EED18E3E" modified="2012-06-13" version="1">MFGSTGERSFADIITSIQYWVIHSITIPSLFIAGWFVSRGLAYDVFGSQGPNEYFTESRQGIPLITDRFNSLEQLDEFSRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1J1K9</accession>
    <name>I1J1K9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100829555</name>
        <name evidence="14" type="ORF">BRADI_5g21140v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580512.1">
        <property type="nucleotide sequence ID" value="XM_003580464.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J1K9"/>
    <dbReference type="FunCoup" id="I1J1K9">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ84483">
        <property type="protein sequence ID" value="KQJ84483"/>
        <property type="gene ID" value="BRADI_5g21140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829555"/>
    <dbReference type="Gramene" id="KQJ84483">
        <property type="protein sequence ID" value="KQJ84483"/>
        <property type="gene ID" value="BRADI_5g21140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829555"/>
    <dbReference type="eggNOG" id="KOG0698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013173_0_1_1"/>
    <dbReference type="OMA" id="IKNATCL"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J1K9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000010">
        <property type="entry name" value="Probable protein phosphatase 2C 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="13">
        <location>
            <begin position="65"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ84483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="316" mass="34510" checksum="0598A5CA56D58940" modified="2012-06-13" version="1">MVGRMERQTASTSCSHSAAGSSSSSSCGGKKRPDILNMIRSATCLHSSSTDTGKGRSKLSSTKVAHGFHLVEGKSGHDMEDYHVAEYKYEKNHELGLFAIFDGHLGDRVPSYLRANLFCNILKEPLFWTNPQEAIKNAYGSTNKYILENAKQLGPGGSTAVTAIVVDGKDMWIANVGDSRAVLCERGAANQLTVDHEPHTTNERQRIEQQGGFVTTFPGDVPRVNGQLAVARAFGDHSLKTHLSSEPDVRHVPINSNIEFVILASDGLWKVMKNQEAVDLVKSTKDPQAAAKRLTTEALARKSKDDISCIVIRFRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1J3A6</accession>
    <name>I1J3A6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100846267</name>
        <name evidence="9" type="ORF">BRADI_5g25960v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580811.1">
        <property type="nucleotide sequence ID" value="XM_003580763.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751450.1">
        <property type="nucleotide sequence ID" value="XM_014895964.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J3A6"/>
    <dbReference type="STRING" id="15368.I1J3A6"/>
    <dbReference type="EnsemblPlants" id="KQJ85262">
        <property type="protein sequence ID" value="KQJ85262"/>
        <property type="gene ID" value="BRADI_5g25960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846267"/>
    <dbReference type="Gramene" id="KQJ85262">
        <property type="protein sequence ID" value="KQJ85262"/>
        <property type="gene ID" value="BRADI_5g25960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846267"/>
    <dbReference type="eggNOG" id="KOG3297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073901_1_1_1"/>
    <dbReference type="OMA" id="FCLSEME"/>
    <dbReference type="OrthoDB" id="10256606at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04330">
        <property type="entry name" value="RNAP_III_Rpc25_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000259">
        <property type="entry name" value="RNA polymerase Rpb7 N-terminal domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013238">
        <property type="entry name" value="RNA_pol_III_Rbc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08292">
        <property type="entry name" value="RNA_pol_Rbc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF03876"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF08292"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="200" mass="22586" checksum="2EAF2279C85CDEBE" modified="2012-06-13" version="1">MFVLSQIEHNLPMPPHLLSRPLVDAIKTELERLFLDKVIANLGLCVSVYDILAVEGGFIFPGEGCSTYKVSFRLLMFRPFIGEVLVGKISGYDEKGLQVSLDFSSDICIPGHLMQYGTVRGEDGRWMLKTEDDDELYLDLDDEIRFLVASIKYPPIPVEQKEDDKPFAPMQINGSIKGDGLGLLAWWAADEEEEEKEEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_4g02080v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ85862"/>
        <property type="gene ID" value="BRADI_4g02080v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="389" mass="40125" checksum="AD7AA06E72D09553" modified="2016-01-20" version="1">MASAAASRPPAPPPPPPPPPATMQWLSPRVSFSLDDAGCGGGGGRDAAAVMGAGKPSADFEFLLAGCSAVSTMLPADELFSGGKLVPLRLPAQTPASCSTARRPPLTPPEPETTNTPKPDQELAKDVSMASEEEGPKVVPARRWRDLLRLRKQQASSSSASSASSTETTKPLRRLLRRGSGGPKPPEQEPSLSLPLLRPDDELSPSPASTTVLHPHLQPSTPSNPHQQHQHQNHHLPPKIRLTPSQQATAPPPPPPPPPPPPSAVAADSPRLNAAGKVVFNGLGRSSSSPSSLAGGRRAHGHRCGGGVGMERSYSAHVRVTPVLNVPVCRKSVSVFGIDRLFSPSSASSAAHAAASKKGKVAKKEAAPVTTAAAATAPSPQISSRALSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EFU2</accession>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RAMP4 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3EFU2"/>
    <dbReference type="EnsemblPlants" id="KQJ86574">
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        <property type="gene ID" value="BRADI_4g06402v3"/>
    </dbReference>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7364" checksum="F48A00F4C25FA963" modified="2016-01-20" version="1">MTTSRRLADKKSAKFQKNITKRGSVPETTVKKGNDYPVGPIVLGFFIFVVIGSSLFQIIRTATSGGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EJJ7</accession>
    <name>A0A0Q3EJJ7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100839340</name>
        <name evidence="3" type="ORF">BRADI_4g13970v3</name>
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    <organism evidence="3">
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EJJ7"/>
    <dbReference type="STRING" id="15368.A0A0Q3EJJ7"/>
    <dbReference type="EnsemblPlants" id="KQJ87863">
        <property type="protein sequence ID" value="KQJ87863"/>
        <property type="gene ID" value="BRADI_4g13970v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87863">
        <property type="protein sequence ID" value="KQJ87863"/>
        <property type="gene ID" value="BRADI_4g13970v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EJJ7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="2">
        <location>
            <begin position="63"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ87863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="298" mass="33475" checksum="00F2FB3883E24F1B" modified="2016-01-20" version="1">MYMKQLAHNGTGRHKLKCTQQYRPHHYHTLLLELSTPTLAQADRPPSTGHTTTTTIIITLGCSSQGLRLWQNMVDLMKKLARMDVDMSGEERHLFSVGFKNTIGAKRASWRILSSLEQKVTTGEQAGGMINDYRKKVEDELRMVCNEILSIIAIHCLPLANSGENIVFFYKMKGDYYRYLAEFSTGTEKKAAADQSLVAYQHAMVVASTELSPVHPIRLGLALNFSVFFYEIMNCHERACQVAKQAFDEATAEINSMGVEGYNDSTLMMQLLKDNLTLWTSELTGGESSKDNDLDMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EXE3</accession>
    <name>A0A0Q3EXE3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_4g41708v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EXE3"/>
    <dbReference type="FunCoup" id="A0A0Q3EXE3">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92110">
        <property type="protein sequence ID" value="KQJ92110"/>
        <property type="gene ID" value="BRADI_4g41708v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92110">
        <property type="protein sequence ID" value="KQJ92110"/>
        <property type="gene ID" value="BRADI_4g41708v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EXE3"/>
    <dbReference type="OrthoDB" id="1534087at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1028">
        <property type="entry name" value="OS08G0174800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="12"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="158"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="383"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="509"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ92110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="808" mass="91818" checksum="47C2F81099D957F6" modified="2016-01-20" version="1">MEAALVSVGAGVMKPLLCKLYKVLKDERGKLKSLRPHVEETRKEMISMEVLADAEQLNSQMRGWRDEVRELSYDMEDCVDDFMARVDHEHDAPKSFFMGLFDKAKKLKPRRRINGKIKELKARAIEASKRQKRYKIDGLSPESSTCDIDPRVHALFVELRVVSIVGPGGLGKTTLTNAVFQSMKSEFSCVAFVSVSRKPDMKNVLREIAEKVKITPDTSSDTEQRLIERLRDHLQEKRYLVVIDDIWSEKAWKTIKDALPNNDSGSRIITTTRNRTLASFCSSQGYYTVSSKSIRSEASCPPHLKEVFERILKKCAGLPLAIITMSSLLADQTAERVWNRALAAVGSSLAKEDGAGEMTKISSLSYFDLPHYMKACLLYLSAYPEDRMIQKKSLIHKWMAEGFIREEKGRSTHEVGESYFNDFINRSLIEPVEGFISDDEVTFGEVEACRVPDIILDFITCKAKEENFMTSFGDAEHGGKHKVRRLSVVNHDNGMATVSMDLSHNFKLDFPALRVLDLEGFWDLKTDHFANIEKLLLLKYLRLGSSSSFSEIPEGIGKLKFLETLDMRGLTIRKLPSTMAQLQRLARLYAIFELDCLSDGIIGQLRSLEELEKVVVSETQQEKFLQELGQLTKLSTLSVHVRQSKLSKKVDNVRCVGTLISSYNLHHLTITYRGNRLRGYLLSLESWRPANPAALRKICIRDYCIGKVPSWIISLGNLRVLHLSDMYCTRPEDMAILGGMPALVALKLDTGFRSLKYFDLRVRCCGTTVEFGEGSMPKLEHLELEFSAHFFFARKIKCAFDRVCELWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FS97</accession>
    <name>A0A0Q3FS97_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_2g00520v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02280.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FS97"/>
    <dbReference type="EnsemblPlants" id="KQK02280">
        <property type="protein sequence ID" value="KQK02280"/>
        <property type="gene ID" value="BRADI_2g00520v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02280">
        <property type="protein sequence ID" value="KQK02280"/>
        <property type="gene ID" value="BRADI_2g00520v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FS97"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FS97">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613:SF62">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="266"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="371"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="441"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="476"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="511"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="581"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="616"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="651"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="686"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="755"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="790"/>
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        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="825"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK02280.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="868" mass="96088" checksum="7B0DB5427304F6B3" modified="2018-03-28" version="2" fragment="single">MVKLLADLLHHTPPSAWPPALASPPLRNRISPSHVSSLLLLPASLSRPGLSRRFLLLLPPSIVSPLCLSLLALSFLSSTSPPSSSSSSPHAASLLLSLASSSLSASSSFSSLSHASSLSAFPPSATAAAGTLLASSYLRLRRARDAAAVLHLSLSAGIAPDQHTASQILFSLVKIRQFALARHLFDGMLHSGVLLDEYVYTAGIRAYCEVRNLDGAKGLVARMQDEGDKVSAVPYNVLIYGLCKNQRVREAVDVKNSMLARGVAADEVTCRTLVYGFCRTEELDMALEMTGDMARLGFVPSEANCSFMLDGLRKKGRVEEAFRLACQLGELRMVPNIFAYNALLNNMCKNGMFSEADRLVNEMSDKGLEPNEVTYAILIHSLCKRGMMDDALCMLDRMREKGVRMTVYPYNSLINCCCKKDDLDMAMGFLSEMVEIGLTPNAASYSPVIAGLCRKGDLSGAVELHRKMAEKGVAWNTYTFTALINGFCKAKKMDEASRLFNKMTESNLEPNEVTFNAVIEGYCLVGDIRKAFQLYDQMMCRGLTPDNYTYRSLISGLCLTDGASKAKEFVADLENNCSVLNKFSLTALLHGFCREGRLTEAYHVWNEMAMWGGKLDLISFTIIVYAALKQHDSEKSCVLFREMKEKGVRPDNVFHTCMINMYSKEGNMVQALNCWDEMIADGHLPNTVTYTALVNNLCKSWHLSSAELLCKEMLASHFLPNSYTFNCFLDYFATEGNLETAKDLYFAMLQGFLANIVSVNTLIKGFCKVGQIQEAIDLISRSTENGFFPDCISYSTVIHELCKKGDINEAIELWNEMLYKGVKPDIVAYNILIRWCNIHGESDKCLGIYIDMVKKGVQPNWHTHRALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G483</accession>
    <name>A0A0Q3G483_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100845079</name>
        <name evidence="3" type="ORF">BRADI_2g24680v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT71195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003568353.4"/>
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        <property type="nucleotide sequence ID" value="XM_024460124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G483"/>
    <dbReference type="STRING" id="15368.A0A0Q3G483"/>
    <dbReference type="EnsemblPlants" id="KQK06120">
        <property type="protein sequence ID" value="KQK06120"/>
        <property type="gene ID" value="BRADI_2g24680v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT71195">
        <property type="protein sequence ID" value="PNT71195"/>
        <property type="gene ID" value="BRADI_2g24680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845079"/>
    <dbReference type="Gramene" id="KQK06120">
        <property type="protein sequence ID" value="KQK06120"/>
        <property type="gene ID" value="BRADI_2g24680v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71195">
        <property type="protein sequence ID" value="PNT71195"/>
        <property type="gene ID" value="BRADI_2g24680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845079"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3G483">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Heat shock protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HSP40/DnaJ peptide-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF539">
        <property type="entry name" value="CHAPERONE DNAJ C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="8"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK06120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="340" mass="37372" checksum="F1138313C826FB0C" modified="2016-01-20" version="1">MGKNPPELYYEVLNIAKDTTSPQEIRAAYRGLVRQWHPDKHPPSSKTEAEARFKAITQAYEALLDQQEDRAVFRARNVDEDGRRRSCASRAENAAGGGLPAMAPRAPCKISATTSSTRKAPAAARECSGQTKVYSSTDVGLGGRRAFAEFSSYVVRKAPPLECKVECTLEELCAGCKKEVKYTRDVVTKNGLIAKKEVTQIIRVKPGWKKGTKVTFEGMGNERPGCLPGDAVFTVSIRKHKAFKRQGDDLVLKAEVPLVSALTGWSFSFRLMSGEKVSWSFRDEVICPGYEKVVKGEGMPVAGGHRGARGDLRVKFDVVFPENLSEERRTGLAEILRGCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GKC8</accession>
    <name>A0A0Q3GKC8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g56713v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GKC8"/>
    <dbReference type="EnsemblPlants" id="KQK10869">
        <property type="protein sequence ID" value="KQK10869"/>
        <property type="gene ID" value="BRADI_2g56713v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10869">
        <property type="protein sequence ID" value="KQK10869"/>
        <property type="gene ID" value="BRADI_2g56713v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GKC8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK10869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="186" mass="21721" checksum="8A3769DAC24472F8" modified="2016-01-20" version="1">MSSRSLALLPSQLPSVQSRPKGAASSSCRCHPWMLSRRRRLWSSRWLPSTNTKPHQRDVFTSPKNPKKTLRFLLSFNSFFSLVFFNLSSHGADRGKKRKPRIWKRRGGSWKIREDITRRRKKSCSKRRTRMVLLLQLCIATRKLDPQYVGLQMMCQQQLLYTFKTRTGLMTAASQFTTSKLSQPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GKT9</accession>
    <name>A0A0Q3GKT9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_2g57817v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GKT9"/>
    <dbReference type="FunCoup" id="A0A0Q3GKT9">
        <property type="interactions" value="1877"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GKT9"/>
    <dbReference type="EnsemblPlants" id="KQK11053">
        <property type="protein sequence ID" value="KQK11053"/>
        <property type="gene ID" value="BRADI_2g57817v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11053">
        <property type="protein sequence ID" value="KQK11053"/>
        <property type="gene ID" value="BRADI_2g57817v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GKT9"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GKT9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000310">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000114">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF284">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="9">
        <location>
            <begin position="294"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="483"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK11053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="790" mass="86735" checksum="B5A7294F1886B625" modified="2016-01-20" version="1">MDAMEVEECPFLANDAKLHAGMCRAFHPAVSKLLAIFPFIEASRPRSKSGIQALCSLHVALDKSKGLLQHCADCSRLYLAITAETVLLKFEKSRSQLQESLRRVESIVTEDIGHKIVEIIGELEEVVFTLDQSEKEAGDEVINLLQRSGKMSSSDSGELEVFHMAALKLGITSSRAALTERRALKKLIEKARSGDDKRKEFVVSYLYNLMRKYSKFFRSEAGDDTDSQGSAPCSPTVLGMDDMYGQYGPCGNGRAFSRQLSSIQSFNSRFGSFNSRLGSFNCRPSGPRSENMSIPPEELRCPISLQLMYDPVIISSGQTYERVCIEKWFNDGHSTCPKTQQLLAHLSLTPNYCVKAMISSWCEQNDFPVPDAPPGSFDVNWRLALSDSQATGCVSVDSFDTSNIKGVKVVPLENGRKEEPANSESGTLDDSSCFEFDMNEGYRNLLLMLNERNNLLNQCRLVEQIRYLLKDDEEARIQMGSNGFAEALVQFLRNSVQDGNEKAQEIGAMALFNLAVNNNRNKGLLLSAGVVDLLEQMTSNPRLTAAATALYLNLSCLPDAKSVIGSSQAVPFLVDRLYNHDGCDTKTSSCKHDALYTLYNLSTHQASIPSLLSAGIVDALHCLFTESSVSEGLGWTEKSLAVLISIAATQAGRKEIMSTPGLISTLAMLLDAGEPTEQEQAVSCLLAMCTADDKCIAPVLQEGVVPSLVSISATGTGRGREKAQKLLKLFREQRQRDGSQPAQQQPLSEAGNGAIVCHRESKPLCKSKSKKLGRTLSSLWKNRSFSLYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GM96</accession>
    <name>A0A0Q3GM96_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">At1g61320/AtMIF1 LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100839039</name>
        <name evidence="2" type="ORF">BRADI_1g02180v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236097.1">
        <property type="nucleotide sequence ID" value="XM_010237795.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GM96"/>
    <dbReference type="EnsemblPlants" id="KQK12207">
        <property type="protein sequence ID" value="KQK12207"/>
        <property type="gene ID" value="BRADI_1g02180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839039"/>
    <dbReference type="Gramene" id="KQK12207">
        <property type="protein sequence ID" value="KQK12207"/>
        <property type="gene ID" value="BRADI_1g02180v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839039"/>
    <dbReference type="OrthoDB" id="592278at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GM96">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35545">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35545:SF27">
        <property type="entry name" value="FBD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="At1g61320/AtMIF1 LRR" evidence="1">
        <location>
            <begin position="173"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK12207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="444" mass="50868" checksum="782C035751E23749" modified="2016-01-20" version="1">MESNHHSNNDNSQLEDEDRLSMLTDDILLSILGKVGVRMSARTSVLSTRWRHLPWLLPELSIDVKDFLSDPDSDPIEANDMEEAMVSLTKATRSFLAKSRKGSTISRLHLQLYLINTFLCDVGPLVGDAIDGGLLKDLDLAVIDETDPIDCSEEDMLQRAQEIEGFFNACPSVLHCLTKLSLYHVSFDKSDMHHVLFECCKHLKHLSLTQCDTGPKALFKIDAPNSKITVLELDKCRFERLELVCLPKLEKLKWFTWVSRFAPLAFGFVPSIGELELTSAATFSQRSFKLSELLRGITGIHTLKLGFEGENVWMQPEMKQLCTAFNKLRKLSVCGIFVEFDILWVMAFLAAAPSIEILHIEVWDHACDPVDEETRRSFHERRNPQWDMQFDETKNCLLKVLEFVGFKLLEQCASPFEYPYFHAQHVYSKWQSNTQFFAYSVFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GXG1</accession>
    <name>A0A0Q3GXG1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="4 14">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BRADI_1g24431v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="4 14"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15695.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GXG1"/>
    <dbReference type="STRING" id="15368.A0A0Q3GXG1"/>
    <dbReference type="EnsemblPlants" id="KQK15695">
        <property type="protein sequence ID" value="KQK15695"/>
        <property type="gene ID" value="BRADI_1g24431v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15695">
        <property type="protein sequence ID" value="KQK15695"/>
        <property type="gene ID" value="BRADI_1g24431v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GXG1"/>
    <dbReference type="OrthoDB" id="337870at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GXG1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043039">
        <property type="term" value="P:tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000001">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000158">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF0">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="TYROSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033724924" description="Tyrosine--tRNA ligase" evidence="16">
        <location>
            <begin position="23"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="119"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK15695.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="590" mass="63622" checksum="25F73F807C0E120D" modified="2018-03-28" version="2" fragment="single">MKHRLRQIGHLSLLALSVGAAAERILHLRRTAGAHQEIWGVHELATMGAETAPAGTSVAAPPRRRIPRSRPCLHGLDTLPRPGGHAMAALPKCPCSPLALGEVAPCGRNRSRVRNMMARSGAVHRPPDLNLDLDEDQQRTGSSGSGQLCSVRTASSSSPTHAAAADSPPSPRFPQTPQPAASTAVRRSVVDVLRERGLVEATTSEALGSSPSAGGQQQQLKAYCGFDPTAESLHLGNLLGLVALSWFRRCGHTAVALVGGATGRVGDPSGKSAERPELDLAAVAANSDAIKSLIAQILGRAPEPSHYSKSGKNAILDENEHALANSCQGLSENVGGKMGSFVILDNYDWWKDITLLDFLKEMGKFARVGTMIAKESVKKRLNSEDGMSYTEFTYQLLQGYDFLYMFKNMGVNVQIGGSDQWGNITAGTDSIRKILQVEGAHGLTFPLLLKSDGTKFGKTEDGAIWLSSKMLSPYKFYQYFFSVPDVDVIRFMKILTFLSLDEILELEKSMKRPGYVPNTVQRILAEEITRFVHGQEGLEEALKATQALRPGAQTQLDSQTIEGIADDVPSCSLPYDQVLKSLLVDLAAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HVV5</accession>
    <name>A0A0Q3HVV5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100829619</name>
        <name evidence="8" type="ORF">BRADI_3g32353v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574276.2">
        <property type="nucleotide sequence ID" value="XM_003574228.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HVV5"/>
    <dbReference type="FunCoup" id="A0A0Q3HVV5">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3HVV5"/>
    <dbReference type="EnsemblPlants" id="KQJ97634">
        <property type="protein sequence ID" value="KQJ97634"/>
        <property type="gene ID" value="BRADI_3g32353v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829619"/>
    <dbReference type="Gramene" id="KQJ97634">
        <property type="protein sequence ID" value="KQJ97634"/>
        <property type="gene ID" value="BRADI_3g32353v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829619"/>
    <dbReference type="OrthoDB" id="5307922at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF88">
        <property type="entry name" value="ADIPOCYTE PLASMA MEMBRANE-ASSOCIATED PROTEIN HEMOMUCIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129277" description="Strictosidine synthase conserved region domain-containing protein" evidence="6">
        <location>
            <begin position="27"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="7">
        <location>
            <begin position="161"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ97634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="372" mass="40258" checksum="A9CDD54F8E20B7C1" modified="2016-01-20" version="1">MPMAPGLGFLTKAVLLVLAPVFISVALYSPKDFSPAAMPPEYSFGADDVSVPASRHDARALDASERVGEGRLPGPEDLAYDAAGGWLYTGCADGWVRRVSVPSGAVKNWAYTGGRPLGVALTGDGGIIVADADKGLLRVGLDKSVELLTDAAEGLRFALTDGVDVATDGTIYFTDASHKHSLRNFILDVLEARPHGRLMSFDPSTRRTTVLARDLYFPNGVALAPDQGSLIFCETIMRMCSRYHIKGDKEGMVERFIDRLPGLPDNVRYDGDGRYWIALSAGRTLLWDMLMGSPLLRKLVYLVNRYVAAVPKSTGGAGTLSVGLDGTPVTMYSDPRLVLATGWFKVGDYLYYGSLQNSYISRIDLTKSFIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IDJ6</accession>
    <name>A0A0Q3IDJ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100824708</name>
        <name evidence="7" type="ORF">BRADI_3g37561v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236721.1">
        <property type="nucleotide sequence ID" value="XM_010238419.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IDJ6"/>
    <dbReference type="FunCoup" id="A0A0Q3IDJ6">
        <property type="interactions" value="999"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3IDJ6"/>
    <dbReference type="EnsemblPlants" id="KQJ98547">
        <property type="protein sequence ID" value="KQJ98547"/>
        <property type="gene ID" value="BRADI_3g37561v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824708"/>
    <dbReference type="Gramene" id="KQJ98547">
        <property type="protein sequence ID" value="KQJ98547"/>
        <property type="gene ID" value="BRADI_3g37561v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824708"/>
    <dbReference type="OrthoDB" id="10251412at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IDJ6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19510">
        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025753">
        <property type="entry name" value="AAA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14363">
        <property type="entry name" value="AAA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="266"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ98547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="523" mass="58546" checksum="BD8BC0E0CF846F07" modified="2016-01-20" version="1">MQGDGSKMLSVGKMLALGALSRLRSSAWIYLAPVAAAFTPIGDLRGYLCRRLQLHRRVRRLLPFLDPFVTVDIAARRDNYAYSSSAGQIKSSDAYTEVLAYLSSVSSRDARQLRAEGAVEGDGFVFSLREGQEVADVFNGVTMWWSSATAAAAPGLHFHGSPHGPPCCRLTFHERHRSLVVDQYLPHVRRRGQEVLFGNRRRRLYTNRNGLNYGSRTNEVWSYIDFDHPTTFDTLAMDPAKKRAIMDDLDDFRNNGDYYHRIGKAWKRGYLLHGPPGTGKTTMIAAMANYLGYDIYDIELTTMHSNNDLRKLFVETTGRSIIVIEDIDCSLDLTGSRARATAGTTFQGWQGDGDLDAYGMRNTKTRDERGNIMTLSGLLNFIDGLWSVHSGERIIVFTTNHLDKLDPALIRRGRMDMHIEMSYCEFEAFKKLAENYLGVDAHPLFDAVRELLRAVEITPADVAECLITSKRSARDADACLGRLLDELKKKAGEKEEQNRVAVDDGTDAGESFCSAAEMIVNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3II98</accession>
    <name>A0A0Q3II98_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g02511v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85936.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3II98"/>
    <dbReference type="STRING" id="15368.A0A0Q3II98"/>
    <dbReference type="EnsemblPlants" id="KQJ85936">
        <property type="protein sequence ID" value="KQJ85936"/>
        <property type="gene ID" value="BRADI_4g02511v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ85936">
        <property type="protein sequence ID" value="KQJ85936"/>
        <property type="gene ID" value="BRADI_4g02511v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3II98"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF39">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ85936.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="70" mass="7688" checksum="9028F1D2E6F8FB60" modified="2018-03-28" version="2">MHPVVADLAKAYAGRLRCLRVNTDENQEVATRYGIRSIPTILIFSKGERKETIIGAVADTALAATVDRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J4L1</accession>
    <name>A0A0Q3J4L1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104582762</name>
        <name evidence="3" type="ORF">BRADI_2g36859v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753715.1">
        <property type="nucleotide sequence ID" value="XM_014898229.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J4L1"/>
    <dbReference type="EnsemblPlants" id="KQK07660">
        <property type="protein sequence ID" value="KQK07660"/>
        <property type="gene ID" value="BRADI_2g36859v3"/>
    </dbReference>
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    <dbReference type="Gramene" id="KQK07660">
        <property type="protein sequence ID" value="KQK07660"/>
        <property type="gene ID" value="BRADI_2g36859v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS05G0137600 PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK07660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="169" mass="18263" checksum="E5C280823C739E11" modified="2016-01-20" version="1">MWGGAQTAPARRLFLSSPDVTLPPPPPRHPRSRLSLKATCASTSTRARLAGAGSSMRRETQPRLAAVQGPPALSLAASAARRDATTGLAFLLFVLALVMSSFLSVAIFSFPAWRALKQLERIAHKLSKVVAEEVPGTLSSLKLSIMEISDITSQLKNLRQSFTINRSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JA03</accession>
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        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g18310v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14723.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="1227"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3JA03"/>
    <dbReference type="EnsemblPlants" id="KQK14723">
        <property type="protein sequence ID" value="KQK14723"/>
        <property type="gene ID" value="BRADI_1g18310v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14723">
        <property type="protein sequence ID" value="KQK14723"/>
        <property type="gene ID" value="BRADI_1g18310v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JA03"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JA03">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Os07g0670000 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000453">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
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    </dbReference>
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        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
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    <dbReference type="InterPro" id="IPR011990">
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        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
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            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="77566" checksum="B414A56FA03FDE53" modified="2018-03-28" version="1">MPPLPQIPLHARAPLSSTAAHHLLDRIPRPTSTLPLLASSPAAFTAAVPSSVPANLPALHGLAIASGLDAFSFVTNSLAARYAKSASSFPSAAKVFHTARARDVSSYNTILSALPDRGEALAFAAWMLRRGEADGVWIVRQLHALASRSGLVADVFVGNALVTAYSRGALLGAARRVFDEMPARDLVSWNAMICGLAQDGDCPTEVILVFLRLLKDGGAAVRPDRISVCSVIPACGSEGKIELGRQVHSFTVKLGVEGKVSIGNVLVAMYYKSGAAGCARKLLKSMDERDVISWTTAISMDGEEDAIELFNGMRQDGVPPNEVTFVALMSALAAGCPARYGQMIHTVCLKTGVSDEAAAANSLITMYAKLRRMDDARTVFDRMPRPEIIAWNALISGYAQNELCNEALQVFSCMVRCLRPNETTFASVLSAVTAVETVSMAYGEMYHCQSLKLGLKVSEYVSGALIDMYAKRGSLEESRKAFDVTVHRSLIAWTAIISAHAKHGNYDTVMNLFDDMVCSGVAPDGVVLLSVLTACRHSGAVNTGREIFDSMPAEHHVEPWPEHYACVIDMLGRAGRLEEAEELMLQMPTGPSVSALQSLLGACRIHGNTSIAERVAGILTETEPTESGAYVLLSNIYAEKGDWGGVAKVRREMREKGVRKEIGFSWVDFGAGESLHLHKFSSDDTTHPCTEEIYRVAEGLGLETKLLKNCLHMEMESIF</sequence>
</entry>
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    <accession>A0A0Q3JRG6</accession>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">BRADI_1g17762v3</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JRG6"/>
    <dbReference type="EnsemblPlants" id="KQK14644">
        <property type="protein sequence ID" value="KQK14644"/>
        <property type="gene ID" value="BRADI_1g17762v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14644">
        <property type="protein sequence ID" value="KQK14644"/>
        <property type="gene ID" value="BRADI_1g17762v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JRG6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK14644.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="99" mass="11540" checksum="CF834EA673EF2751" modified="2018-03-28" version="2">MLGRRRISPRTRIRKVCKSRDFMSHLTCLPIAMFKRHLHEICHEVKPILENVCTAAVTPFFIAQSVNDLTIIRVQDHPTAAQIDRKLKPQFSRTHLCHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JW15</accession>
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            <fullName evidence="2 3">Uncharacterized protein</fullName>
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        <lineage>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Vogel J.P."/>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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        </source>
    </evidence>
    <sequence length="564" mass="63065" checksum="621A7ABBF7EEA2D9" modified="2016-01-20" version="1">MAAAVSPLASPGPDLDRLPRPSLASPWDSKALCKRAETCELESGAFLIDPALLPTLEDLLLEIYAMLRPKPVDYEQRHIMIDVFNKIAKDVCGGKNNRFPVVEAFGSFTMDLFTAKSDLDLSVNFSADRDGEFDRNKKISVIRKFAKVLRQHQSRGRCYGVLPVVNAIVPVLKVTDKGTGVECDISVENKDGMSRSMIFKLVSSIDERFRILCYLMKFWAKSHDVNCPRDRTMSSMAIISLVAFHLQTRRPPILPAFSRLLKDGADIASIQRNVSLFEGFGSRNKESVAELFVSLMSKLLSVQGLWEQGLCASNLEGSWILKMTWDRGIGNLAVEDFLDRNQNFARSVGKVEMQTICECLRDTVCKLTDFFKGDIDAPTLKILIFGALKEDKPVSRTSPKLVETKRKRDARLDPGNGQRPQKKRRHAAQPGHAADQTDACLPTPTMFMPPPPQMHLPRSQFARPPQHPAPPPSRFPYGPPLPLPLPHLHPAPHMVGQPQHGNFMHPNPGIQLHQQAQHNMFAPLQARQQAFNGFHPGYGFDGAPQIPYDPNSGLPYINPNYRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3P7G6</accession>
    <name>A0A0Q3P7G6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">NADH:ubiquinone oxidoreductase-like 20kDa subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BRADI_5g23847v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42608"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Stromal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3P7G6"/>
    <dbReference type="FunCoup" id="A0A0Q3P7G6">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3P7G6"/>
    <dbReference type="EnsemblPlants" id="KQJ84937">
        <property type="protein sequence ID" value="KQJ84937"/>
        <property type="gene ID" value="BRADI_5g23847v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ84937">
        <property type="protein sequence ID" value="KQJ84937"/>
        <property type="gene ID" value="BRADI_5g23847v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3P7G6"/>
    <dbReference type="OrthoDB" id="772270at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12280:FF:000003">
        <property type="entry name" value="NAD(P)H-quinone oxidoreductase subunit K, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01356">
        <property type="entry name" value="NDH1_NuoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 19" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12 19" id="KW-0408">Iron</keyword>
    <keyword evidence="13 19" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 19" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-0618">Plastoquinone</keyword>
    <keyword evidence="7" id="KW-0874">Quinone</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="20">
        <location>
            <begin position="70"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQJ84937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="252" mass="28406" checksum="9578D993ACB0F2E8" modified="2016-01-20" version="1">MAKRSLGMVLTEYLEKKEEEKDSIETVMSLIEFPLLDQTSSNSVISTTPNDLSNWSRLSSLWPLLYGTSCCFIEFASLIGSRFDFDRYGLVPRSSPRQADLILTAGTVTMKMAPSLVRLYEQMPEPKYVIAMGACTITGGMFSTDSYSTVRGVDKLISVDVYLPGCPPKPEAVIDALTKLRKKISREIVEDQIRSQNKNRCFTTSHKLYVRRSTHTGTYEQELLYQSPSTLDISSEIFYKSKSIVPSYKLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3P9H6</accession>
    <name>A0A0Q3P9H6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g00565v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3P9H6"/>
    <dbReference type="EnsemblPlants" id="KQJ85609">
        <property type="protein sequence ID" value="KQJ85609"/>
        <property type="gene ID" value="BRADI_4g00565v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ85609">
        <property type="protein sequence ID" value="KQJ85609"/>
        <property type="gene ID" value="BRADI_4g00565v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3P9H6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ85609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="125" mass="13545" checksum="6011F4FFA55B89B6" modified="2016-01-20" version="1">MLGCLEGHDGAPGHGSSVNLRLCRPNQQQQPVGDLERSKTGSLCGRWPSAWRAPSALRVSIWLRIGMGPSGGDCFSWEFDPGICLCRSRIHLAGLHAQCSGELDEGRGRGARRQETGRSGRRSGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PFJ2</accession>
    <name>A0A0Q3PFJ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enhancer of polycomb-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100838503</name>
        <name evidence="9" type="ORF">BRADI_4g15730v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the enhancer of polycomb family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237619.1">
        <property type="nucleotide sequence ID" value="XM_010239317.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PFJ2"/>
    <dbReference type="STRING" id="15368.A0A0Q3PFJ2"/>
    <dbReference type="EnsemblPlants" id="KQJ88129">
        <property type="protein sequence ID" value="KQJ88129"/>
        <property type="gene ID" value="BRADI_4g15730v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63435">
        <property type="protein sequence ID" value="PNT63435"/>
        <property type="gene ID" value="BRADI_4g15730v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63436">
        <property type="protein sequence ID" value="PNT63436"/>
        <property type="gene ID" value="BRADI_4g15730v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63437">
        <property type="protein sequence ID" value="PNT63437"/>
        <property type="gene ID" value="BRADI_4g15730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838503"/>
    <dbReference type="Gramene" id="KQJ88129">
        <property type="protein sequence ID" value="KQJ88129"/>
        <property type="gene ID" value="BRADI_4g15730v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63435">
        <property type="protein sequence ID" value="PNT63435"/>
        <property type="gene ID" value="BRADI_4g15730v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63436">
        <property type="protein sequence ID" value="PNT63436"/>
        <property type="gene ID" value="BRADI_4g15730v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63437">
        <property type="protein sequence ID" value="PNT63437"/>
        <property type="gene ID" value="BRADI_4g15730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838503"/>
    <dbReference type="OrthoDB" id="435275at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3PFJ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032777">
        <property type="term" value="C:piccolo histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Enhancer of polycomb-like N-terminal" evidence="8">
        <location>
            <begin position="574"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="724"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="724"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ88129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="827" mass="93671" checksum="5281BDD085D68C22" modified="2016-01-20" version="1">MKPLPRSHRNRPPLSAPLPEPMPAVGTRRSTRVFVPKVPQPQPSEPARVRRSGKRLAISNSHWLRWESKNAFHDNEHGEKPKPQTPMQPRSPPTRSFGIVYSRKRCRRLPAEPKDDTRFGIVFTRKDKRSKVAPFREDTSSDLAAIPCSLSREFASRIGFLDAHFLTPVAGVASQAGDDLLVVLIDTSFSGSTHQFLRLLLPVLRWMRHGQQSKIWNLASFLSSAAVVTIFASHGLHFVKLQHQRASALLLRTMVHCGWCELHDANQSQPVLSVNFSAVPSYFQSLHSVIDLHSIYVPAGIRRSMGLVGGAEEMYTEDPLEADSWSPSTVDAEPAVDLRCDEPCRVVQDYVPLEQVAGVVMHGLKLKKHQRKRSSMRHPVNWHRLAARFPDKAIGMKQGTLTSQTELSPALTGSEICLEPLQPKPALEISLDLLENMDDSDVSTPMGSNGKQKRSFKSPIERTNERLALSEVRQNIDHFCCKANLLIIQADRCWREEGAVVMLELSNSNGWCVAVKLRGVTRVSLKPSEQRFYVVNRHTHAYVWAIEDGWKLEFSDKWDWLLFKELHIEGRERNSQEKMIPIPGVHEVSDGMGGIVTDPFSRPVPDYIRMVDDEVGRALSRDSVYDLDSEDEQWLIQLNHEASDRRSSNLNHISYEDFEKIITLFEKDIYNNPGVTTDVDQLLSRYPSLGKDDNVLSIYEYWTSKRYKKGAPLIRTFQGAPVRRGRQSQKSSVKKKRSFMRQRSQPGRGKPGIFLQDNAEEEALRRVVEAERAAKHAVETAVRLRSRAQSLMANANLATYKSVMALRIAEAASISNSCRDLVWRTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QSE9</accession>
    <name>A0A0Q3QSE9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">adenylate dimethylallyltransferase (ADP/ATP-dependent)</fullName>
            <ecNumber evidence="10">2.5.1.112</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_2g13410v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in cytokinin biosynthesis. Catalyzes the transfer of an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>dimethylallyl diphosphate + ADP = N(6)-(dimethylallyl)adenosine 5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36327"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:73533"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.5.1.112"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dimethylallyl diphosphate + ATP = N(6)-(dimethylallyl)adenosine 5'-triphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36331"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:73532"/>
            <dbReference type="EC" id="2.5.1.112"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.112" evidence="10"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04418.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3QSE9"/>
    <dbReference type="FunCoup" id="A0A0Q3QSE9">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3QSE9"/>
    <dbReference type="EnsemblPlants" id="KQK04418">
        <property type="protein sequence ID" value="KQK04418"/>
        <property type="gene ID" value="BRADI_2g13410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04418">
        <property type="protein sequence ID" value="KQK04418"/>
        <property type="gene ID" value="BRADI_2g13410v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3QSE9"/>
    <dbReference type="OrthoDB" id="775260at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009824">
        <property type="term" value="F:AMP dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052622">
        <property type="term" value="F:ATP/ADP dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.890:FF:000002">
        <property type="entry name" value="Adenylate isopentenyltransferase 5, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.890">
        <property type="entry name" value="Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF74">
        <property type="entry name" value="ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK04418.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="312" mass="33813" checksum="26021686AE0D9DB2" modified="2018-03-28" version="1">MGATATGKSKLAVDLALQFGGEVINSDKIQLHDGLPIVTNKITPNEQSGVPHHLIGGLHPDSDYSPSDFRRDATRAVENVLSRGALPIVAGGSNRYLEALLDGPSSSFRRRFECCFVWVDAGGDVALLEEYVRQRVDAMVEQGLVEEVRGFFREDGDYSRGIRRAIGVPEMDAYFRMEAAGELIRAGVLEAAVEQIKRNTCVLARRQVGKIRRLSGLPGWNIRRVHVGRVLALKVAGEKRKEDAGAERELWEKDVLGPAARTVEVFLAKGAGERGGAGVGAAVEWYGRGLQFVEAASAVSPRFHGRKAAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R3G1</accession>
    <name>A0A0Q3R3G1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g39440v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08026.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R3G1"/>
    <dbReference type="EnsemblPlants" id="KQK08026">
        <property type="protein sequence ID" value="KQK08026"/>
        <property type="gene ID" value="BRADI_2g39440v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08026">
        <property type="protein sequence ID" value="KQK08026"/>
        <property type="gene ID" value="BRADI_2g39440v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3R3G1"/>
    <dbReference type="OrthoDB" id="583206at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186:SF3">
        <property type="entry name" value="OS09G0267800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK08026.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="783" mass="89030" checksum="781AF6C9BF51520E" modified="2018-03-28" version="1">MHQRLQAVHAWCTLLRLEQCIDKNYLTCAATAKSPPTHRCHVNRLNRDRCPDVNFNPSGSNVSWSSGVGPMASFTEAITVRVVEGLSEEFGPFKHVIKVDLKPSPMPTADGGGITSIKHQLAIAAAKELGLLDQEYNRLKQEYEAVQYYSYGSNDLASYNLEHYVKDKIVPWISRKLSTEKYLLVVKNLRWWIEPSNFTRDVRLPPPSWENSHWFISTITKYAGKRSKSSGDQVISIHVHAVVVVLALYSLHHSAEHIFNMTRQENKEYWHHIALLCFHYATLIFTKHSPVVPVTSDELIHQWAAQGILPRMPINGEEEETGNCTYSSRCAYMHRVGRIILEAFQKYSLLRLPYSPANNADRATGTAAQFFSYHGLITEDITVDALFENKKNWITFVDDHGWHVSREWLSPKENRGATALILRGCSQQSLIFSKLDHFLLKLCFLRILDLSYTPLKSLPSSVGSLLYLRLLSLRGCHDLETLSSTNTTSSNSPLSTLYQLEILDMNGVPFSHLTQDVANQKSNLIFLDMSYSQVTSFPPNFFQGMSNLEELVLVNCSKLVELPPSMVALSSLTTLEIAGTQIKYFPKKIFDEMQKLQSLKLIDNKKLFSLLESISRAQGLIGLHIEGYESSMVEEIKLEGHPTLTSFILIRAPHIRRLSLRGCRKLESVKFKNLDALEDLDLSATAIKELLAGIPNVPHLRRLILVGVPSLRRFPWHMLGRLPDVFYLDHCIEGNGNHSDHVARIRVSDPRFFHSFCENFCGFSKRRTVFPIFLCPSRTMPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RH47</accession>
    <name>A0A0Q3RH47_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g04650v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RH47"/>
    <dbReference type="FunCoup" id="A0A0Q3RH47">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3RH47"/>
    <dbReference type="EnsemblPlants" id="KQK12578">
        <property type="protein sequence ID" value="KQK12578"/>
        <property type="gene ID" value="BRADI_1g04650v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12578">
        <property type="protein sequence ID" value="KQK12578"/>
        <property type="gene ID" value="BRADI_1g04650v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3RH47"/>
    <dbReference type="OrthoDB" id="589312at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044997">
        <property type="entry name" value="F-box_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32153">
        <property type="entry name" value="OJ000223_09.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK12578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="49456" checksum="3B75C56033CEC9A1" modified="2016-01-20" version="1">MKNKKGSRGTRNRMLRLSTMLSRFDMSVGNIARRHNVRARYDNRVARYNDALANVTDKVLYARNREIPINKLRVRFFLRRYECLSISKAVARTMATQKVNDAEFVITTEKVFARCIDADLHWYAKLFKTCLGDCPAAFAGLTRLWLRNMRFDEPDILNTCKRLESLRLTCCDAGVRLVLQVEHPQLVELAIDSGKFGTVVLNCLPKLQHLSYTNWSYQDPLCFESVPQLSKLSLAKMGIRSTKNLEFSQFLADVPSITTLHLDFLSEKIWVLPEHPTLLAPVLGKLQIAKLVNLPEGCDIAWTMFILEAIVTDENFPREHGYCEKANVQWQPCTFDFKHKNLVQLTIYGFQPELNFVQYVRRVMEAAVNLREISLHDREVCGDCRDLDPKIKNKVCPSRYPRTCVEDMLRAEITRELGMASTALIHFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S0X1</accession>
    <name>A0A0Q3S0X1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g43995v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3S0X1"/>
    <dbReference type="FunCoup" id="A0A0Q3S0X1">
        <property type="interactions" value="324"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK18674">
        <property type="protein sequence ID" value="KQK18674"/>
        <property type="gene ID" value="BRADI_1g43995v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18674">
        <property type="protein sequence ID" value="KQK18674"/>
        <property type="gene ID" value="BRADI_1g43995v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3S0X1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33075:SF7">
        <property type="entry name" value="OS02G0303350 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33075">
        <property type="entry name" value="OS02G0499800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK18674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="683" mass="77589" checksum="0A46985D692F7923" modified="2016-01-20" version="1">MHRWRLPITVPPSRRPNFFMVASFGRCKFCRIEESVEEFRVCHLRDRTFRFYVTNKNIGFHIAGLNSFTCTNFVVYFHLWGFGGPDFIKEFAAWEKEELLKWESPKKAQSPPRASRSFVQVAAQARTTSLALMISDTRSSLMANFVVDPVPFVLGLFDIIDAEGRPQHSRYHIRGRIEPQNEDVAIVTLQPPPNPDALFEETRDWLVRNGPHQHNGITFTFTEHNRAQNWRGFTYNQEVWLMILGFHLDIWTSEHIANAIADWGKLILWDRTVNNYSRIIIKVKVVDLSLVPYSLLLSHGSDFQGETWVAPVYILHQAFMSALPTDEDARPGDGDTPHPLPVLPFEEDHGNNANHGPIIPDLNMEADFWGPWDQALHNAGANAHDLFFLPEGNQAANVQHIHLEDVLLQMPQDQDMHALHFIAAPDAQPADQMQLPTAASENIHHDLQNVHAGHNNMLNDGQENMQIGRVIIPPFNHPQENFPALYGPHLTKPNLELLHTSTDSNEIWIKHFCPRSNRNSISVPQPWVDFFTASLISPESFEWASKVLLSNMWTIFSENAPLSKDFYIPDTCPSSSPLKCDLTARAAMISKGYLTPHAPTKLPCLPDIASTSAIRGKREKKAPLVVTEVRRSERINKKNGGYKHNTCVDRHCLACSAKPPEISKKIIRNLNERFGLAKNDIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGN7</accession>
    <name>A0A2K2CGN7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription repressor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ovate family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100835807</name>
        <name evidence="9" type="ORF">BRADI_5g11645v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
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                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="10">cv. Bd21</strain>
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    </evidence>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
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                <person name="Bruce D."/>
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                <person name="Shu S."/>
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                <person name="Wang M."/>
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                <person name="Garvin D.F."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
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            <strain evidence="3">cv. Bd21</strain>
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            <end position="47"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62056"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
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        <name evidence="2" type="ORF">BRADI_4g06565v3</name>
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="2">Bd21</strain>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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            <strain evidence="3">cv. Bd21</strain>
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            <begin position="62"/>
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            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
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    </evidence>
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            <dbReference type="EMBL" id="PNT62660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="221" mass="23158" checksum="E1AF2007B4C858C5" modified="2018-03-28" version="1">MAATSERSCQKFILVQQLQLKNIHTHNLKINHIFAWKPKRGEQGQTLHLPELAPSWDAAKERGMPSERPMACCATPSMSDPPLASDSHRGRLESAALLPPPARPAPPRGSGWEGGGAADQEEAGRKMQAAAARHREEGEGREGGGGTSSRGKRRDGGGSGAILLASAESGGGRARWRQHSGGMAAALPGPARRVLPPPRRGSMPGAAATRSEIHGGAGQCQ</sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="BRADI_4g11597v3"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="44"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63116"/>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
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            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT63229">
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    </dbReference>
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            <end position="213"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576654.1">
        <property type="nucleotide sequence ID" value="XM_003576606.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CRZ7"/>
    <dbReference type="EnsemblPlants" id="PNT64800">
        <property type="protein sequence ID" value="PNT64800"/>
        <property type="gene ID" value="BRADI_4g33174v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821641"/>
    <dbReference type="Gramene" id="PNT64800">
        <property type="protein sequence ID" value="PNT64800"/>
        <property type="gene ID" value="BRADI_4g33174v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821641"/>
    <dbReference type="OrthoDB" id="273345at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CRZ7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070042">
        <property type="term" value="F:rRNA (uridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000440">
        <property type="entry name" value="Os09g0479300 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019446">
        <property type="entry name" value="BMT5-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF26">
        <property type="entry name" value="FERREDOXIN-FOLD ANTICODON-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10354">
        <property type="entry name" value="BMT5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="25S rRNA (uridine-N(3))-methyltransferase BMT5-like" evidence="1">
        <location>
            <begin position="24"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT64800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="257" mass="29240" checksum="43BFFBC7B0FE987C" modified="2018-03-28" version="1">MTRRRKTGRGVKWLSHYSSEQSILVVGDGDFSFSLALATAFGSGVNIVATSLDSYEALIGKYSKAELNVMELKTMGAKVLHHINAKSMMRHSFLETRRFHRIVFNFPHSGFKGSEYEMHVVISHRELVKGFFTNARYLLQPYGEIHISNKIGYPYDSWNIEQLALESSLTMIGRVSFQKQDYPGYNQKRGDGARSDQPFPLGYCCTFKFQDHKEVQERSVVYRCYICHISSYYHYDCVGCGATLGLGNGGPLHLAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CUG1</accession>
    <name>A0A2K2CUG1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g00555v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CUG1"/>
    <dbReference type="EnsemblPlants" id="PNT65672">
        <property type="protein sequence ID" value="PNT65672"/>
        <property type="gene ID" value="BRADI_3g00555v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65672">
        <property type="protein sequence ID" value="PNT65672"/>
        <property type="gene ID" value="BRADI_3g00555v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CUG1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT65672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="230" mass="24782" checksum="8C1CE0AC86FCC310" modified="2018-03-28" version="1">MLSRGPATFSSGRAFFPASTRLSLSLSPYCICSGFPTPLLPGISRSRTDASCRSPLSLLRPPSPFVPPAVSAAAAGRYCRSHLSPSLDLLSPASAKRPRALLSPNPTRISFYNRGSGSYCCSGRAAPQRKPPRRGFAQRDPSGSSELGVGMRHVGVEPWRRWRVRRLVVVQAPEQRGMDACHGLELRGGVARSSMGNSQAPTRLLCESQAELMTALKHLGLNCIVVNLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CVV5</accession>
    <name>A0A2K2CVV5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ninja-family protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">ABI-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g07965v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a negative regulator of abscisic acid (ABA) response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Ninja family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CVV5"/>
    <dbReference type="STRING" id="15368.A0A2K2CVV5"/>
    <dbReference type="EnsemblPlants" id="PNT66163">
        <property type="protein sequence ID" value="PNT66163"/>
        <property type="gene ID" value="BRADI_3g07965v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66163">
        <property type="protein sequence ID" value="PNT66163"/>
        <property type="gene ID" value="BRADI_3g07965v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CVV5"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031307">
        <property type="entry name" value="Ninja_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012463">
        <property type="entry name" value="Ninja_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032310">
        <property type="entry name" value="NLS_NINJA_AFP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31413">
        <property type="entry name" value="AFP HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31413:SF31">
        <property type="entry name" value="NINJA-FAMILY PROTEIN AFP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07897">
        <property type="entry name" value="EAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16136">
        <property type="entry name" value="NLS_NINJA_AFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ethylene-responsive binding factor-associated repression" evidence="6">
        <location>
            <begin position="25"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT66163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="137" mass="14638" checksum="58AEA27E31EEE4B9" modified="2018-03-28" version="1">MASRDFLGRFGEGRGAERAGGGGEAEETVELSLGLSRGGCFGASSGRDAKKPWLQLARSSSIGSMCSLPAGGGGAGSENDKLAAATPAPAPLMRTSSLPAETEEERWRRREMQSLKRLQAKCKRLERRNSMNPLTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CWQ0</accession>
    <name>A0A2K2CWQ0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g11594v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CWQ0"/>
    <dbReference type="EnsemblPlants" id="PNT66449">
        <property type="protein sequence ID" value="PNT66449"/>
        <property type="gene ID" value="BRADI_3g11594v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66449">
        <property type="protein sequence ID" value="PNT66449"/>
        <property type="gene ID" value="BRADI_3g11594v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CWQ0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="101"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="223" mass="24136" checksum="D2790EE122027298" modified="2018-03-28" version="1">MYEGGYNGPGGALRAPNGSHFYSSMFYPFGMRHCRQLLRWAVRLSGEPAAARARGAGHGAGNRGVLRVAVRETVVREELRAASCRASGLESALMERDKAALLPVREERQGACHNRGARRSPPPRLPEPPGLNRAGRGSPSRGRTPVRAGGGLERLGRDYQLRPPRPASPGDTGRHGRTDARGGYGGRRSHRPAHLLAGTLPLNLALEIRFVPLICKLLIRVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CZG6</accession>
    <name>A0A2K2CZG6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g27185v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CZG6"/>
    <dbReference type="EnsemblPlants" id="PNT67416">
        <property type="protein sequence ID" value="PNT67416"/>
        <property type="gene ID" value="BRADI_3g27185v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67416">
        <property type="protein sequence ID" value="PNT67416"/>
        <property type="gene ID" value="BRADI_3g27185v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CZG6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT67416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="71" mass="7573" checksum="393580B33E6311D3" modified="2018-03-28" version="1">MCWTQTPGVSFLSLEYCPGITYSCQVKCFTIITGVADGTNVAVAAPPTPLSPWNPFLKAGGVSCEKCRKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D0V7</accession>
    <name>A0A2K2D0V7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g33713v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D0V7"/>
    <dbReference type="EnsemblPlants" id="PNT67918">
        <property type="protein sequence ID" value="PNT67918"/>
        <property type="gene ID" value="BRADI_3g33713v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67918">
        <property type="protein sequence ID" value="PNT67918"/>
        <property type="gene ID" value="BRADI_3g33713v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D0V7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT67918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="47" mass="5343" checksum="E8B3EC9FFAE6E490" modified="2018-03-28" version="1">MQPVPNAPEVDKTCIDNKKAPPITQLHDQRCYISAFTQCLMTSRNHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D183</accession>
    <name>A0A2K2D183_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g35347v3</name>
    </gene>
    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D183"/>
    <dbReference type="STRING" id="15368.A0A2K2D183"/>
    <dbReference type="EnsemblPlants" id="PNT68056">
        <property type="protein sequence ID" value="PNT68056"/>
        <property type="gene ID" value="BRADI_3g35347v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68057">
        <property type="protein sequence ID" value="PNT68057"/>
        <property type="gene ID" value="BRADI_3g35347v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68056">
        <property type="protein sequence ID" value="PNT68056"/>
        <property type="gene ID" value="BRADI_3g35347v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68057">
        <property type="protein sequence ID" value="PNT68057"/>
        <property type="gene ID" value="BRADI_3g35347v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D183"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="ExpressionAtlas" id="A0A2K2D183">
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    </dbReference>
    <dbReference type="GO" id="GO:0003690">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT68057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="758" mass="81985" checksum="28D632977AFEE7DE" modified="2018-03-28" version="1" fragment="single">VEREDRRSGGGGGVGGFESKRRLAPPPGRPPPKRRALSATRRFQPGCGRETAAHLVVRSGGEDGLRVGASPSHGGFSMLERATTCARLSCAGDGGTEEGGLLLEAAIANAGLVLPEKGGASSADGFRPKATVVKPSDASGFAGFVVTRGRGAWSDKLGEKELVPILQSPLNRDMVRARARPAHSLGLDTVAPLDRGGGGEMGLQLDIQPAVGGLDASEEVIATHDSGCMACRCAMDVVEATNKDSLDGPIVCQELEEGEIAATLDIVEATGNDALDVPIEAHELEEGEIAATMDIVKVVNKDPLDGAVERQGLEKGEVDATMDIVESKNKRTLDGAMEVQELEEDEIAVPMHILESTNKQNFDGAVEVQELEESEIDATMDIVEAMNKNMLVGAVEGQELEGKIAAKGHGQEPENCDIATKGHGQKPVDGDTATRYGQEPENGEIAAKGHGQEPVDGETVTRSDHIVQESQFVVDFIPRNSTDSFMRSKMRLADRKTLRPRVKANQTKSDHIVKESQFAADGIPRDSTGGIMRSNTRLTARKTVWPPVNANHMSLLTTPDRPFSTIRESFATMKKFKAAQSKPVHVNTASTSALANKEKRTFEFLFVSKDKAKDKKAFYLEGDDILKAIAVHEGKLLLFVNVPSGVRSVWHHRQHVGHNTDARSKVRMMSRRFQFLCRFIALAVEQRLLEVLRVDLAADKVIKEFPDCTKHESIVVKLLELKSATNFYSEQSSPLLVFTAPIGLALTPARTIMVYILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D2I1</accession>
    <name>A0A2K2D2I1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_3g41275v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
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        <property type="gene ID" value="BRADI_3g41275v3"/>
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        <property type="gene ID" value="BRADI_3g41275v3"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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        <property type="entry name" value="BPM1-6"/>
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        <property type="entry name" value="BTB/POZ_dom"/>
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        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
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        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS08G0523800 PROTEIN"/>
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        <location>
            <begin position="116"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT68494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="280" mass="31038" checksum="EE8EC47575C927AE" modified="2018-03-28" version="1">MFDSCFVPFKVKLPYDQTENLAADHAVSSKDVSAGGYLWIFSLPTTPTTPTPTTDHSSGWSWCVKRSELESLYMVDGWITIACGVIVVRDDSLSVPPSDIGTHLSRLLDCAAADGSDVSFVVGGKKFPAHRAVLAARSPVFKAELFGSMAEASMSDITLTDIAPATFEIFLRFMYTDTLPEDGDSPIEMYKHLLAVADRYAMDRLKLMCAKKLWDDVSVDTVAETLSHAETYRCAELKTKCITFFAKEKNFRKAVLTDGFVRLVHKFPAIVAELREKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D5P3</accession>
    <name>A0A2K2D5P3_BRADI</name>
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        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100844781</name>
        <name evidence="1" type="ORF">BRADI_3g58610v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT69601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT69601">
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        <property type="gene ID" value="BRADI_3g58610v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g58610v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="44476" checksum="7D2D1132A5DCB031" modified="2018-03-28" version="1">MGSYWHTPYIHTQHTVCPQPFPSLQETFFSFLDPCLADTPSWYPVIGVPGRGSRVQGMGECSSSSSVGEYRCWEELLPDALGLVFRKLPLREVLTVVPRVCKSWGRVVSGPYCWQEIELEEWSQQQQSRPEQLARLVETLVRRSSGSCRRLSVSSGLPCDPLFSFIGDHARALRTLEIPRSEISDAIVEAVAPRLPNLTFLDISSCTKLGARALEAFGEHCRSLAGLRRVMHPTDVAGKACQRDEARAIARGMPALRHLEMGYMLVTTDAVLEVLARCRKLEFLDLRGCWAVDDGLILQQGRRRQPELKVLGPRVDDCFENGYLEECSDDDEDDEDVWELMEDDDDYYYYAVVGSDDDEEIIWDQGQGLENLEVRFYGGGFSESYAGFDWPPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D989</accession>
    <name>A0A2K2D989_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g19065v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D989"/>
    <dbReference type="EnsemblPlants" id="PNT70848">
        <property type="protein sequence ID" value="PNT70848"/>
        <property type="gene ID" value="BRADI_2g19065v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70848">
        <property type="protein sequence ID" value="PNT70848"/>
        <property type="gene ID" value="BRADI_2g19065v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D989"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT70848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="81" mass="8986" checksum="E9D8F257B20A6E86" modified="2018-03-28" version="1">MAMAKGQKCASSRANVEVCCVAKITIVSCSQDHKEMHEAMCVYHVVNRDNIRIPKLSTMLQIIVRRPSTATLRCPVSHSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DBX6</accession>
    <name>A0A2K2DBX6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g35635v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DBX6"/>
    <dbReference type="FunCoup" id="A0A2K2DBX6">
        <property type="interactions" value="493"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DBX6"/>
    <dbReference type="EnsemblPlants" id="PNT71796">
        <property type="protein sequence ID" value="PNT71796"/>
        <property type="gene ID" value="BRADI_2g35635v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71796">
        <property type="protein sequence ID" value="PNT71796"/>
        <property type="gene ID" value="BRADI_2g35635v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DBX6"/>
    <dbReference type="OrthoDB" id="781822at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057235">
        <property type="entry name" value="DUF7913"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057237">
        <property type="entry name" value="DUF7915"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33913">
        <property type="entry name" value="ALEURONE LAYER MORPHOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33913:SF1">
        <property type="entry name" value="DRBM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25500">
        <property type="entry name" value="DUF7913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25502">
        <property type="entry name" value="DUF7915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7913" evidence="1">
        <location>
            <begin position="12"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7915" evidence="2">
        <location>
            <begin position="153"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT71796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="606" mass="67583" checksum="C6410AB1388F19BA" modified="2018-03-28" version="1">MEVMNFDGTSGEVVQMEEAVSMLLEHLVGPVLPRAATDVTPAEMERAVARQIHTAVILYNYYHRSLSPQLAFADVKRFLLCASHSVGEDLFPFSNVVHEKNPGDDVKPSVTDRAAMDACEIAEALDASQDFPEMSLWPIAKVAVLLLDPTRKKCVWSIIEKEFDASAGNSYSSKQLLLHRTSKIFALSVCSLCIVLLPYGKLMVLKGMKHANLHILDEDLTYSLSTERTITKLFIVEYEQTTNRELTEVPLQELVSSMSGPLFVNDPFPKTSPVVEHYHILPYKKSLLQFLHRERPSDYAVHEIAESMDQQEAKNKSMMQKGTKKVSTAKQKQAIKLVDTSPDGENPVRENDPLVVDLDTSKQTTKYRNIKEAAAASGGKLILQAGVQVDKEKTQSSDITPDVFPTKAPSVGHVTENIILEGQNMGASENSGGIMENNNDQMYHSLQLIQKMRDDFLHKKHILAERSAQCDMDIQTILSEGKMTSIVKSIMDKYKNNSSNKAEATTSSLSGGGGQTLTTKRLKLREAPLLGKKCQELDDICRDSNWMLPRYTVLPSKDGMFDACVNLRGFDFDMRINGDRCMTPYEARCSAAAHMIVELQKKAEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DFS2</accession>
    <name>A0A2K2DFS2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g53975v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DFS2"/>
    <dbReference type="FunCoup" id="A0A2K2DFS2">
        <property type="interactions" value="319"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73131">
        <property type="protein sequence ID" value="PNT73131"/>
        <property type="gene ID" value="BRADI_2g53975v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73131">
        <property type="protein sequence ID" value="PNT73131"/>
        <property type="gene ID" value="BRADI_2g53975v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DFS2"/>
    <dbReference type="OrthoDB" id="690579at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT73131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12765" checksum="77DE71920343F766" modified="2018-03-28" version="1" fragment="single">DCDHLFFTCPFSVSCWGSLDIYWDMSMDIRDRVRAAKASFSGPSFIMIFLCAAWHIWKQRNSFIFDRSPPSLSSWFAGFKQELFLLSHRIKENHRSILLAWLESLVIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGH8</accession>
    <name>A0A2K2DGH8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g57732v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGH8"/>
    <dbReference type="EnsemblPlants" id="PNT73385">
        <property type="protein sequence ID" value="PNT73385"/>
        <property type="gene ID" value="BRADI_2g57732v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73385">
        <property type="protein sequence ID" value="PNT73385"/>
        <property type="gene ID" value="BRADI_2g57732v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DGH8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT73385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="50" mass="5602" checksum="24C5B7924A249760" modified="2018-03-28" version="1">MWPPWRAARSQSVLPLPLSLSLFFLCPFPSFLERGSGRALLILPSVSLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DNB4</accession>
    <name>A0A2K2DNB4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g37821v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DNB4"/>
    <dbReference type="EnsemblPlants" id="PNT75771">
        <property type="protein sequence ID" value="PNT75771"/>
        <property type="gene ID" value="BRADI_1g37821v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT75772">
        <property type="protein sequence ID" value="PNT75772"/>
        <property type="gene ID" value="BRADI_1g37821v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75771">
        <property type="protein sequence ID" value="PNT75771"/>
        <property type="gene ID" value="BRADI_1g37821v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75772">
        <property type="protein sequence ID" value="PNT75772"/>
        <property type="gene ID" value="BRADI_1g37821v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DNB4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="108" mass="11625" checksum="6CDB712BF186F846" modified="2018-03-28" version="1">MRRSMQHAGEPQGRRVAGAAVPPLSRGAAGGGCTSAVSPFSPSSFYFRLFRFFPVDVPLTSRFRFAGIGDFLPCACFRRCCLPPSACVSLDGGATVALRRFSACRFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DNK5</accession>
    <name>A0A2K2DNK5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_1g39221v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase A chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
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                <person name="Vogel J.P."/>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Bd21</strain>
        </source>
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    <reference evidence="3" key="3">
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                <consortium name="EnsemblPlants"/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">Bd21</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">cv. Bd21</strain>
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    <dbReference type="PANTHER" id="PTHR47723">
        <property type="entry name" value="OS05G0353850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47723:SF17">
        <property type="entry name" value="OS05G0353850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="170"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT77573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="332" mass="36714" checksum="AA9E2A4F15EE55AE" modified="2018-03-28" version="1">MESEEEEAAPELEAMEIEEEASAPPAPPPPRIRVLGTDEEKEQRLSFFTCKASKDDARGKALDDAELTWGIVADHAFELNRRAWVANKGLGPLLRYSIAKPLLALPRVDKDDKADGEVREIFNLNSLALALEIVGCEIEMASCVAGSQPFRPRRIMVRWQPPRAGWFKLNFDGSVYHDGSGRASIGGAIRDCNGRVLAAFAERTEHSTVGIVEARAMIRGLRLALGCFLGRLVVEGDDLVLVQLLRGEETQTRIPLPMQEEILGLLARFSACEVQHIHREGNQVAHVLCQQAYHRPGVWMGGLVPRAAWEKAEDDLHGVAHERLCKTKAMAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMU4</accession>
    <name>I1GMU4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC104581877</name>
        <name evidence="8" type="ORF">BRADI_1g07230v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cytochrome c oxidase subunit 6A family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229196.1">
        <property type="nucleotide sequence ID" value="XM_010230894.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMU4"/>
    <dbReference type="FunCoup" id="I1GMU4">
        <property type="interactions" value="863"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GMU4"/>
    <dbReference type="EnsemblPlants" id="KQK12984">
        <property type="protein sequence ID" value="KQK12984"/>
        <property type="gene ID" value="BRADI_1g07230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581877"/>
    <dbReference type="Gramene" id="KQK12984">
        <property type="protein sequence ID" value="KQK12984"/>
        <property type="gene ID" value="BRADI_1g07230v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104581877"/>
    <dbReference type="eggNOG" id="KOG3469">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122515_2_1_1"/>
    <dbReference type="OMA" id="FEAKHED"/>
    <dbReference type="OrthoDB" id="5947505at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.95.10:FF:000002">
        <property type="entry name" value="Cytochrome c oxidase subunit Via"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.95.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001349">
        <property type="entry name" value="Cyt_c_oxidase_su6a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036418">
        <property type="entry name" value="Cyt_c_oxidase_su6a_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02046">
        <property type="entry name" value="COX6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81411">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK12984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="97" mass="10813" checksum="CA35DBE5905787AB" modified="2012-06-13" version="1">MASAAARSGLRSLAARAKAPAPARRRMSSSVHDDAYETAKWEKITYAGIVTCTLLAAWNLSKGHPHFDEPAPYPYLHIRNKEFPWGPDGLFEVKHDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1GN41</accession>
    <name>I1GN41_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100846040</name>
        <name evidence="3" type="ORF">BRADI_1g08080v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559406.1">
        <property type="nucleotide sequence ID" value="XM_003559358.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GN41"/>
    <dbReference type="FunCoup" id="I1GN41">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13104">
        <property type="protein sequence ID" value="KQK13104"/>
        <property type="gene ID" value="BRADI_1g08080v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846040"/>
    <dbReference type="Gramene" id="KQK13104">
        <property type="protein sequence ID" value="KQK13104"/>
        <property type="gene ID" value="BRADI_1g08080v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846040"/>
    <dbReference type="eggNOG" id="ENOG502R3B0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030845_0_0_1"/>
    <dbReference type="OMA" id="QSYRENW"/>
    <dbReference type="OrthoDB" id="586448at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046533">
        <property type="entry name" value="DUF6598"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065:SF91">
        <property type="entry name" value="DUF6598 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065">
        <property type="entry name" value="OS07G0486400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20241">
        <property type="entry name" value="DUF6598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6598" evidence="2">
        <location>
            <begin position="167"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK13104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="437" mass="49450" checksum="C504B2775C24553E" modified="2012-06-13" version="1">MDVDSEEVGRKRETYHGSEMAGGSRITSRKITGTKWPIIERMDASAKVSREKYMKRLVAKWNAENPDAAPLVLEELTEQQRTSLKEEDLAREFVLVEAYVASRMEEYSRVEELDPNQDNDDRVYQEDRERWERKWAKEFGSFGDKTQIPAMCFTDDPMFHITRDLPSMQIFSIKVKEVHENLQWPLDVYGIIAVRDDIDHNRNVIFERTRDNCQTLNEQDPYLVLTGPARAPVILFEPVRLDVMLKVKCTEESEDKDLSLLVRRYECCESINYQASHGELVANSCASSKKFKSKLSTLELTCGIVVSSVEATISVRIVEGSWPDGFSGRFTAFTACVSHMKVLLLNSGDGNVLVSADGTIELSRRVLSVESFGELTICAAACGGSKEVERELFFKPLKSGRSAQVLNIGACKMEITVAWSLFPLSYPTRRIHSSKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPA3</accession>
    <name>I1GPA3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6737 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100840163</name>
        <name evidence="3" type="ORF">BRADI_1g11610v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK13653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003559489.4"/>
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    <dbReference type="STRING" id="15368.I1GPA3"/>
    <dbReference type="EnsemblPlants" id="KQK13653">
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        <property type="entry name" value="DUF6737 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6737" evidence="2">
        <location>
            <begin position="97"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK13653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="178" mass="19126" checksum="732E9803B562283A" modified="2012-06-13" version="1">MGALYLLPSTPPPFCSGCRGPAGFSLPRGLAATTPLPSPSASSCARLRLAGGRQRAGAARGGGKEGSKSGGAEFFREDGVVDDMDGYLNYLSLEYDSVWDTKPAWCQPWTILLSGAVAVACSWVLIHSALITGGVSFVICAWWYIFLYSYPKAYTEMISERRKKVSSGAEDTYGMEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRG1</accession>
    <name>I1GRG1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100846888</name>
        <name evidence="8" type="ORF">BRADI_1g18680v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF2/ABP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="FunCoup" id="I1GRG1">
        <property type="interactions" value="2597"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GRG1"/>
    <dbReference type="EnsemblPlants" id="KQK14803">
        <property type="protein sequence ID" value="KQK14803"/>
        <property type="gene ID" value="BRADI_1g18680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846888"/>
    <dbReference type="Gramene" id="KQK14803">
        <property type="protein sequence ID" value="KQK14803"/>
        <property type="gene ID" value="BRADI_1g18680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846888"/>
    <dbReference type="eggNOG" id="KOG3152">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OMA" id="TRKHNDF"/>
    <dbReference type="OrthoDB" id="287393at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GRG1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
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        <property type="entry name" value="ABT1/ESF2_RRM"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="52"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="203"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK14803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="253" mass="29210" checksum="1427CC93A398C17F" modified="2012-06-13" version="1">MAETKEGEYYTEEDMGDEEEEEVQGPGSGGEEEEEGGGKKRKRPLDGFSKRGVCYLSRVPPHMNPSHVRQMFSKYGEVQRIYFVPESQGHRKHSNVRAKAYSEGWIEFAKKSVAKRVANLLNGEQIGGKKRSSFYYDIWNIKYLRKFKWDDLVGEIAEKTHIREQKLNLEITAAKKQRDHYLSNAEKSRTQKIIRERIRKKQKTEGKESNNVHESKIDRPIRQQTRPVEERGPKTKPKLSKNILAGVFGGPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSU7</accession>
    <name>I1GSU7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100837436</name>
        <name evidence="10" type="ORF">BRADI_1g22940v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560019.1">
        <property type="nucleotide sequence ID" value="XM_003559971.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSU7"/>
    <dbReference type="FunCoup" id="I1GSU7">
        <property type="interactions" value="313"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GSU7"/>
    <dbReference type="EnsemblPlants" id="KQK15459">
        <property type="protein sequence ID" value="KQK15459"/>
        <property type="gene ID" value="BRADI_1g22940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837436"/>
    <dbReference type="Gramene" id="KQK15459">
        <property type="protein sequence ID" value="KQK15459"/>
        <property type="gene ID" value="BRADI_1g22940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837436"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059471_1_0_1"/>
    <dbReference type="OMA" id="PPRMDSW"/>
    <dbReference type="OrthoDB" id="1911220at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044653">
        <property type="entry name" value="AZF1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45988">
        <property type="entry name" value="C2H2 TYPE ZINC FINGER TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45988:SF32">
        <property type="entry name" value="OS07G0588600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK15459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="244" mass="24020" checksum="20D2A9ED69774137" modified="2012-06-13" version="1">MDPSAAVSVLSIVEEAASRGKSPVPPPPSPPRMDSWGARGGRGSRRRGPGSGSGSLGGGGAESEEEYLALCLLMLARGVRGDGDGDVKGAGAAAGAAATKGYECSVCGKVYASYQALGGHKTSHRKPPAPAPAASEEASGGAAVAAAAAEAKVHRCSLCLRTFPSGQALGGHKRLHYEGGSAAGDGTKEGAGVKAKAAAALLRDFDLNLPAAAPSTTAGGDDAEAESAPPEAKRARLQVLLAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GV00</accession>
    <name>I1GV00_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100828466</name>
        <name evidence="2" type="ORF">BRADI_1g29240v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563209.1">
        <property type="nucleotide sequence ID" value="XM_003563161.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GV00"/>
    <dbReference type="EnsemblPlants" id="KQK16555">
        <property type="protein sequence ID" value="KQK16555"/>
        <property type="gene ID" value="BRADI_1g29240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828466"/>
    <dbReference type="Gramene" id="KQK16555">
        <property type="protein sequence ID" value="KQK16555"/>
        <property type="gene ID" value="BRADI_1g29240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828466"/>
    <dbReference type="eggNOG" id="ENOG502QVPS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055730_1_0_1"/>
    <dbReference type="OMA" id="DHHWEKD"/>
    <dbReference type="OrthoDB" id="723894at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK16555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="26823" checksum="649214B22E4950B1" modified="2012-06-13" version="1">MARRPQNGGCVRCCLGFLLKFLAFLQAFAAVSALLYAAWILSRWSRHHHLHLHHLLPDLWFACAVMAAGLFYCALLLAGYVAAEINSGCCLCFYTALAMAMMLVEAAFASQIFLNQQWIQDLPYDRTGELNNLVSFVQNNLDVCKWAAIATLATQALSLFLAMVLRAMVSTTNVDYDSDEDFVVIRRPLLLAQGAPAYLPTTTDATGVHPDLWSSRMRQKYGLNSSDYTYNTLDQNAVPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2I7</accession>
    <name>I1H2I7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100832406</name>
        <name evidence="13" type="ORF">BRADI_1g53670v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Cd(2+)(in) + ATP + H2O = Cd(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:48775"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Zn(2+)(in) + ATP + H2O = Zn(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20621"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561282.1">
        <property type="nucleotide sequence ID" value="XM_003561234.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2I7"/>
    <dbReference type="FunCoup" id="I1H2I7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H2I7"/>
    <dbReference type="EnsemblPlants" id="KQK20301">
        <property type="protein sequence ID" value="KQK20301"/>
        <property type="gene ID" value="BRADI_1g53670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832406"/>
    <dbReference type="Gramene" id="KQK20301">
        <property type="protein sequence ID" value="KQK20301"/>
        <property type="gene ID" value="BRADI_1g53670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832406"/>
    <dbReference type="eggNOG" id="KOG0207">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_6_1_1"/>
    <dbReference type="OMA" id="LDCSNCA"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H2I7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008551">
        <property type="term" value="F:P-type cadmium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016463">
        <property type="term" value="F:P-type zinc transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02079">
        <property type="entry name" value="P-type_ATPase_HM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000022">
        <property type="entry name" value="Putative cadmium/zinc-transporting ATPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000043">
        <property type="entry name" value="Putative cadmium/zinc-transporting ATPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051014">
        <property type="entry name" value="Cation_Transport_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01512">
        <property type="entry name" value="ATPase-IB2_Cd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085">
        <property type="entry name" value="CADMIUM/ZINC-TRANSPORTING ATPASE HMA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085:SF8">
        <property type="entry name" value="CADMIUM_ZINC-TRANSPORTING ATPASE HMA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0104">Cadmium</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="359"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="669"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="696"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="12">
        <location>
            <begin position="24"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK20301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="819" mass="85717" checksum="9344DDC7F271FA6D" modified="2012-06-13" version="1">MSGGELRRGALEESLLLTGRTQWDKTYLDVLGVCCSAEVALVERLLSPLAGVRTVSVVVPSRTVVVQHDPAAVSQSHIVKVLNGAGLEASVRAYGSSGIISRWPSPYILASGVLLLASSLSWLLPDPLHHLQWLALAAAFVGAPPMLLRGFAAASRLTLDINVLMLIAVAGAAALGDYTEAGAIVFLFTTAEWLETLACTKASAGMSSLMSMIPPKAVLAETGQVVNVSDIGVGAVVAVRAGEVVPVDGVVVDGQSEVDERSLTGESYPVPKQPQSEVWAGTLNLDGYIAVRTSALAENSTVARMEKLVEEAQNSRSKTQRLIDSCAKYYTPAVVVMGAGVALPPLLLGAGDPERWFRLSLVLLVSACPCALVLSTPVATFCALLTAARMGVLIKGGDVLESLGEITAVAFDKTGTITRGEFSIAAFHVVADKVQITHLLYWVSSIESKSSHPMAAALVEYARSKSIQPKPENVAEFRVIPGEGVYGEIHGRHIYIGNKRAALARGSCHTVPEKKDGDLKGVSVGYVICDGDLVGAFSLSDDCRTGAAEAIQELTAMGIKSVMLTGDSAEAAAHAQEQLGGALEELHWDLFPEDKVRLVAALKARAGPTMMVGDGMNDAPALAMADVGVSMGVSGSAAAMETSHATLMSSDVLRVPEAVRLGRRARRTIAVNLVASVGIKAAVLVLAVAWTPALWAAVLADVGTCLLVVLNSMRLLGEGNRGGGGKKKKEEACRATARSLEMRRAQLLLNADDASNAASTTVVVERPGGGGGREKCHCCHRENESPESSVAIDIIRVDERPEEVRKIAACAPAKGGADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2U7</accession>
    <name>I1H2U7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Galectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100842271</name>
        <name evidence="17" type="ORF">BRADI_1g54650v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Possesses hydroxyproline O-galactosyltransferase activity. Transfers galactose from UDP-galactose to hydroxyproline residues in the arabinogalactan proteins (AGPs). Is specific for AGPs containing non-contiguous peptidyl hydroxyproline residues. Utilizes UDP-galactose solely as sugar donor. The addition of galactose onto the peptidyl hydroxyproline residues in AGP core proteins represents the first committed step in arabinogalactan polysaccharide addition. AGP glycans play essential roles in both vegetative and reproductive plant growth.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557425.1">
        <property type="nucleotide sequence ID" value="XM_003557377.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2U7"/>
    <dbReference type="STRING" id="15368.I1H2U7"/>
    <dbReference type="EnsemblPlants" id="KQK20460">
        <property type="protein sequence ID" value="KQK20460"/>
        <property type="gene ID" value="BRADI_1g54650v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842271"/>
    <dbReference type="Gramene" id="KQK20460">
        <property type="protein sequence ID" value="KQK20460"/>
        <property type="gene ID" value="BRADI_1g54650v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842271"/>
    <dbReference type="eggNOG" id="KOG2287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017063_2_0_1"/>
    <dbReference type="OMA" id="NGYFTAH"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990714">
        <property type="term" value="F:hydroxyproline O-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010405">
        <property type="term" value="P:arabinogalactan protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000005">
        <property type="entry name" value="Hydroxyproline O-galactosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000071">
        <property type="entry name" value="Hydroxyproline O-galactosyltransferase GALT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF245">
        <property type="entry name" value="OS07G0194800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Galectin" evidence="16">
        <location>
            <begin position="165"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="39"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK20460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="653" mass="72754" checksum="C9F2B7CE3F43BA80" modified="2012-06-13" version="1">MPTASRQRLCRAALLVAGAGYLAFLLLFEVPSLPFSSSSSSSAAASQQLPTHSSRRRELEASASTSPFSPARPSKPAFPAAGSPAPSPPPLPIFSSLLVLPRSNATATPFDGTAADAFAAAKPHLTHLRTAAASASVAASPPPSSPTCPASISLHADKLPANGVRTVELPCGLAVGSHVTVVARPRAARPEYDPQIAERKDGKTPLMVSQFMVELVGTKVVDGEAPPRILHFNPRIRGDYSGKPVIEMNSCYRMQWGRSQRCEGFASRPAEETVDDQLKCEKWIRDDDNKSEESKMKWWVKRLIGRPKDVHISWPYPFAEGKLFVLTLTAGLEGYHVNVDGRHVASFPYRTGYTLEDATGLSLNGDIDIESIFASSLPNSHPSFSPERYLEMSEQWRAPPLPTEPVELFIGILSAANHFAERMAVRKSWMMYTRKSSNIVARFFVALNGKKEVNAELKREAEFFHDIVIVPFMDSYDLVVLKTIAIAEYGVRVIPAKYVMKCDDDTFVRIDSVLDQVKKVQSDKSVYVGSMNYFHRPLRSGKWAVTYEEWPEEVYPNYANGPGYVISADIASYIVSEFDNQTLRLFKMEDVSMGMWVEKFNNTRRPVEYRHDVRFYQAGCYDGYFTAHYQSPQHMICLWRKLQSGSTHCCNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1H447</accession>
    <name>I1H447_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BSD2 cysteine rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836490</name>
        <name evidence="3" type="ORF">BRADI_1g58600v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557655.1">
        <property type="nucleotide sequence ID" value="XM_003557607.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H447"/>
    <dbReference type="FunCoup" id="I1H447">
        <property type="interactions" value="2019"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H447"/>
    <dbReference type="EnsemblPlants" id="KQK21079">
        <property type="protein sequence ID" value="KQK21079"/>
        <property type="gene ID" value="BRADI_1g58600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836490"/>
    <dbReference type="Gramene" id="KQK21079">
        <property type="protein sequence ID" value="KQK21079"/>
        <property type="gene ID" value="BRADI_1g58600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836490"/>
    <dbReference type="eggNOG" id="ENOG502S25V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152807_1_0_1"/>
    <dbReference type="OMA" id="GVNSEDH"/>
    <dbReference type="OrthoDB" id="2019540at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057453">
        <property type="entry name" value="BSD2_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15852">
        <property type="entry name" value="PLASTID TRANSCRIPTIONALLY ACTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15852:SF51">
        <property type="entry name" value="PROTEIN BUNDLE SHEATH DEFECTIVE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25436">
        <property type="entry name" value="BSD2_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD2 cysteine rich" evidence="2">
        <location>
            <begin position="63"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK21079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="133" mass="13683" checksum="7B6C61FDBF71D544" modified="2012-06-13" version="1">MAATSNLTATAASAAPTALRTSAPPSPTFISLRPISRCSRLHSVKTKATEKGQGGKKPVKAYSLVCADCEGNGAIACGQCKGSGVNSEDHFNGRFKEGAMCWLCRGKREVLCGSCNGAGFLGGFMSTADDTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H5E6</accession>
    <name>I1H5E6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-mRNA processing factor 4 (PRP4)-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100846442</name>
        <name evidence="6" type="ORF">BRADI_1g62257v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557864.1">
        <property type="nucleotide sequence ID" value="XM_003557816.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228616.1">
        <property type="nucleotide sequence ID" value="XM_010230314.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H5E6"/>
    <dbReference type="STRING" id="15368.I1H5E6"/>
    <dbReference type="EnsemblPlants" id="KQK21663">
        <property type="protein sequence ID" value="KQK21663"/>
        <property type="gene ID" value="BRADI_1g62257v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK21664">
        <property type="protein sequence ID" value="KQK21664"/>
        <property type="gene ID" value="BRADI_1g62257v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846442"/>
    <dbReference type="Gramene" id="KQK21663">
        <property type="protein sequence ID" value="KQK21663"/>
        <property type="gene ID" value="BRADI_1g62257v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21664">
        <property type="protein sequence ID" value="KQK21664"/>
        <property type="gene ID" value="BRADI_1g62257v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846442"/>
    <dbReference type="eggNOG" id="KOG0272">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_20_1"/>
    <dbReference type="OMA" id="LNEPICY"/>
    <dbReference type="OrthoDB" id="540662at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000892">
        <property type="entry name" value="Os03g0355200 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000763">
        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein PRP4-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.110">
        <property type="entry name" value="Pre-mRNA processing factor 4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036285">
        <property type="entry name" value="PRP4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158230">
        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="5">
        <location>
            <begin position="83"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="212"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="254"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="295"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="337"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="379"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="421"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="464"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK21663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="509" mass="56719" checksum="906828079825C062" modified="2012-06-13" version="1">MENPRPPPPAITPPMAPLPIHPPIAPIPAPPRAPVPAAPSTSGDDDDVEYEVSDDHRAARERHERAVQELLQRRRAYAMAVPTNDSAVRARLRRLGEPITLFGEREMERRDRLRALMVRLEAEGQFDRLLRAQEDEQGASEEEAEQIQYPFYTEGTKELLQARVDIAMYSLPLAKARVERAKRRLGDPDEDPEAEADLVVKQAGEFVLDCSEIGDDRPLTGCSFSRDASMLATSSWSGIIKVWSMPQITKIATLKGHTERATDVAFSPVDNCLATASADKTAKLWNSDGSLLMSFDGHLDRLARLAFHPSGKYLGTASFDKTWRLWDINTGKELLLQEGHSRSVYGVSFHPDGSLAASCGLDAFARIWDLRSGRSYCHLEGHVKPVLGVSFSPNGYLVATGSEDNFCRIWDLRARKMLYSIPAHKSLISHVKFEPQEGYYLATSSYDTKAALWSTRDYKPIKSLAGHESKVTSLDISGDGQQIVTVSHDRTIKIWSCRSSTEDNEMELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1H5R7</accession>
    <name>I1H5R7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">EGF-like calcium-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC100825752</name>
        <name evidence="21" type="ORF">BRADI_1g63210v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasmic vesicle</location>
            <location evidence="16">Clathrin-coated vesicle membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Prevacuolar compartment membrane</location>
            <topology evidence="17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VSR (BP-80) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228654.1">
        <property type="nucleotide sequence ID" value="XM_010230352.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H5R7"/>
    <dbReference type="FunCoup" id="I1H5R7">
        <property type="interactions" value="309"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H5R7"/>
    <dbReference type="EnsemblPlants" id="KQK21802">
        <property type="protein sequence ID" value="KQK21802"/>
        <property type="gene ID" value="BRADI_1g63210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825752"/>
    <dbReference type="Gramene" id="KQK21802">
        <property type="protein sequence ID" value="KQK21802"/>
        <property type="gene ID" value="BRADI_1g63210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825752"/>
    <dbReference type="eggNOG" id="ENOG502QSX2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031082_1_0_1"/>
    <dbReference type="OMA" id="RCPMKHE"/>
    <dbReference type="OrthoDB" id="10045365at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02125">
        <property type="entry name" value="PA_VSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000178">
        <property type="entry name" value="vacuolar-sorting receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000001">
        <property type="entry name" value="Vacuolar-sorting receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF1">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094360" description="EGF-like calcium-binding domain-containing protein" evidence="19">
        <location>
            <begin position="22"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like calcium-binding" evidence="20">
        <location>
            <begin position="512"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQK21802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="627" mass="69764" checksum="0301508B4B7377EC" modified="2012-06-13" version="1">MMGPGWCAVVVLLAMTEAAAGRFVVEKNSVRVTSPEGLKGKYECAIGNFGVPQYGGTLHGWVVYPKVNRQGCKEFDVSFKDKEHRSGARPNFVLIDRGECFFTTKAWNAQIAGAAAVLVVDSKDEPLITMDNPDDTGTKHLENITIPSVLITKKLGEDLKKSAEKGEMVSVLLDWRESLPHPDERVEYEFWTNSNDECGPKCDMQMDFVKSFRGTAQVLEQKGYTQFTPHYITWYCPEAFVVSKQCKSQCINHGRYCAPDPEQDFNKGYDGKDVVFQNLHQVCVFKVANDTGKPWLWWDYVHDFAIRCPMKEKKYTHECASHVIKSLGLDIEKIDKCVGDPEADEENPILKAEQDAQIGHGKRGDVTILPTLVVNNRQYRGKLDKGAVLKAICSGFEETTEPTICLSEDVQTNECLENNGGCWLDNDNNVTACRDTFRGRVCECPIVKGVKFVGDGYTNCEASGIGRCEINNGGCWKETKNGKTISACSHEESNGCKCPEGFKGDGIKSCEDIDECKMKTACQCTGCSCENTWGSYECSCAGGDSMLYMREQDTCISKQAVSSVGWSFMWVIFFGLVFAGVGAYAVYKYRLRSYMDSEIRAIMAQYMPLDSQEGANQQQHVAHAGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1HGB6</accession>
    <name>I1HGB6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GBF-interacting protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100840916</name>
        <name evidence="3" type="ORF">BRADI_2g16237v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567874.1">
        <property type="nucleotide sequence ID" value="XM_003567826.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HGB6"/>
    <dbReference type="FunCoup" id="I1HGB6">
        <property type="interactions" value="890"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HGB6"/>
    <dbReference type="EnsemblPlants" id="KQK04832">
        <property type="protein sequence ID" value="KQK04832"/>
        <property type="gene ID" value="BRADI_2g16237v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840916"/>
    <dbReference type="Gramene" id="KQK04832">
        <property type="protein sequence ID" value="KQK04832"/>
        <property type="gene ID" value="BRADI_2g16237v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840916"/>
    <dbReference type="eggNOG" id="ENOG502QRB3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019431_0_0_1"/>
    <dbReference type="OMA" id="QQPFAGN"/>
    <dbReference type="OrthoDB" id="762072at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009719">
        <property type="entry name" value="GIP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46445">
        <property type="entry name" value="RNA POLYMERASE II DEGRADATION FACTOR-LIKE PROTEIN (DUF1296)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46445:SF3">
        <property type="entry name" value="RNA POLYMERASE II DEGRADATION FACTOR-LIKE PROTEIN (DUF1296)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06972">
        <property type="entry name" value="GIP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBF-interacting protein 1 N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="784"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="784"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="819"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="846"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK04832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="859" mass="91661" checksum="63C04860C0EF51C0" modified="2012-06-13" version="1">MSGGGAGGGGKGAAAAGPVPQASRKLVQGLKEIVNRPDAEIYAALRECGMDPDEAVSRLLSQDTFQEVKSKRDKKKEVKETPEPRSRAANNATNRGVRGAPDRGGRNSFAHTSSTDNATARSSVSGPGMLSTNSIQKQTVPSSSVNKTMVADGPSTQPQPSSGFQHGWSGTPGQLSMADIVKMGRPPQTQGKPSTKTVVTADKGYAGQYPSLPSTVNQNLKQSASSVPPTELDQGLPSAQDVVLVKDHSHPAADNKPKYDNDWSPQDDPVSGNQSSLPETSGDPSFYEAALHPSTLVADEVYSHENSYLDGNISASLRSVNASERHLDHYGGNSEYNDGSLQNSNTYLPQKHSRIQDDVEEEPNADLPSSANFQGLSLHDEETSTTKFAEDNPAVIIPDHLQVANTGCAGLSFGSFESGAFSGLLPSKSIDNNVELPVEEESEPVDQIDTRDQDYYDSAAVNLSTNENLDNIIGANTENLDVPSVPQPDVPRQEILDDPSGVQYNLPSVSSHAYSNPAQPNAMEAMQGNNQAHSFNHLTSLLQTNTLHSNLLGSNMSPLRDLDFSLSPLLAAQSMGTRYNSAAPTTTGQAISMQEPLKSGVFSNNQSTQNLPSTSIQTGPPLPQQLTVHPYSQPTLPIAPFTNMMGYPYLAQNYPAYLPSAFQQAYSSNGQFHQSAAAVPGAGMKYAMPQYKGNLSATNLQQQQPSSVLGYGGFGSSSNLPANFNLNQNATSASTNAGFDEALGAQYKDANQYMAALQQQGDNSAMWLHGAGSRTAAALPPTQFYGYQGQSQQGALRQAQQQPQQPSQFGGHGYPAFYHSQSGMTQEHHPQNPSEASLNGYQAAPSQQSHQSWQQHTNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HGI5</accession>
    <name>I1HGI5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100827467</name>
        <name evidence="12" type="ORF">BRADI_2g16850v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the Aux/IAA family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567914.1">
        <property type="nucleotide sequence ID" value="XM_003567866.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HGI5"/>
    <dbReference type="FunCoup" id="I1HGI5">
        <property type="interactions" value="271"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HGI5"/>
    <dbReference type="EnsemblPlants" id="KQK04925">
        <property type="protein sequence ID" value="KQK04925"/>
        <property type="gene ID" value="BRADI_2g16850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827467"/>
    <dbReference type="Gramene" id="KQK04925">
        <property type="protein sequence ID" value="KQK04925"/>
        <property type="gene ID" value="BRADI_2g16850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827467"/>
    <dbReference type="eggNOG" id="ENOG502SMVB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049393_1_0_1"/>
    <dbReference type="OMA" id="GKSEVGC"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000078">
        <property type="entry name" value="Auxin-responsive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF173">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="11">
        <location>
            <begin position="158"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK04925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="269" mass="27863" checksum="623641AE55B58B97" modified="2012-06-13" version="1">MPPPHLEARDYIGLGAAATAPAASSSCSSSASAGEAAGPHLALRLGLPGCESPGRGADPAVDAALTLGPAPATNRAGGAKRGFADSLDRGAAAASAAAAAGGVREKEKGVAEAAAGAPPAAKAQVVGWPPVRNYRKNTLAASATKTKAEGEGRSEAECCYVKVSMDGAPYLRKVDLKTYSSYENLSLELEKMFTCFITGKSSSCKTSTRDRLTDGSRADALQDQEYVLTYEDKDADWMLVGDLPWDLFTTTCRKLRIMRGSDAAGMAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1HMC6</accession>
    <name>I1HMC6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="5 12">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100821500</name>
        <name evidence="13" type="ORF">BRADI_2g37400v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="5 12"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569006.1">
        <property type="nucleotide sequence ID" value="XM_003568958.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HMC6"/>
    <dbReference type="STRING" id="15368.I1HMC6"/>
    <dbReference type="EnsemblPlants" id="KQK07743">
        <property type="protein sequence ID" value="KQK07743"/>
        <property type="gene ID" value="BRADI_2g37400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821500"/>
    <dbReference type="Gramene" id="KQK07743">
        <property type="protein sequence ID" value="KQK07743"/>
        <property type="gene ID" value="BRADI_2g37400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821500"/>
    <dbReference type="eggNOG" id="KOG2446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017947_4_0_1"/>
    <dbReference type="OMA" id="DWYRQLW"/>
    <dbReference type="OrthoDB" id="5831190at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HMC6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000002">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000018">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000031">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000048">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="8 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK07743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="567" mass="62337" checksum="55AD8F970E59F21A" modified="2012-06-13" version="1">MASPALICDTEQWKALQAHVGAIQKTHLRDLMTDAGRCKAMTAEFEGIFLDYSRQQGTVETMDKLFKLAEAAKLKEKIEKMFKGEKINTTENRSVLHVALRAPRDAVINSDGVNVVPEVWSVKDKIKHFSETFRSGSWVGATGKALTNVVSVGIGGSFLGPLFVHTALQTDPEAAESAKGRQLRFLANVDPVDVARSIKDLDPETTLVVVVSKTFTTAETMLNARTLKEWIVSSLGPQAVSKHMIAVSTNLKLVKEFGIDPNNAFAFWDWVGGRYSVCSAVGVLPLSLQYGFPIVQKFLEGASSIDNHFHTSSFEKNIPVLLGLLSVWNVSFLGYPARAILPYSQALEKLAPHIQQLSMESNGKGVSIDGVRLPFEAGEIDFGEPGTNGQHSFYQLIHQGRVIPCDFIGVIKSQQPVYLKGETVSNHDELMSNFFAQPDALAYGKTPEQLHSEKVPENLIPHKTFQGNRPSLSFLLSSLSAYEIGQLLAIYEHRIAVQGFIWGINSFDQWGVELGKSLASQVRKQLHASRMEKKPVEGFNPSSAGLLARFLAVEPSTPYNTTVLPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HN56</accession>
    <name>I1HN56_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100830616</name>
        <name evidence="9" type="ORF">BRADI_2g39730v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566725.2">
        <property type="nucleotide sequence ID" value="XM_003566677.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HN56"/>
    <dbReference type="FunCoup" id="I1HN56">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HN56"/>
    <dbReference type="EnsemblPlants" id="KQK08117">
        <property type="protein sequence ID" value="KQK08117"/>
        <property type="gene ID" value="BRADI_2g39730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830616"/>
    <dbReference type="Gramene" id="KQK08117">
        <property type="protein sequence ID" value="KQK08117"/>
        <property type="gene ID" value="BRADI_2g39730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830616"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055032_1_0_1"/>
    <dbReference type="OMA" id="ANDRVGQ"/>
    <dbReference type="OrthoDB" id="252020at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HN56">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061574">
        <property type="term" value="C:ASAP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12365">
        <property type="entry name" value="RRM_RNPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001153">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034201">
        <property type="entry name" value="RNPS1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481:SF6">
        <property type="entry name" value="OS05G0105900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481">
        <property type="entry name" value="RIBONUCLEIC ACID BINDING PROTEIN S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="97"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="176"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="9"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="381"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK08117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="410" mass="45546" checksum="628CBC22D7BA162F" modified="2012-06-13" version="1">MANRAAAGRSPSPLSFSSSSSRSPSPFSSSSSSSRARSPPRRSSSAARRPRSSPPPPQKRKGGRRSPSPDKPSPPPPSPPPAPPLRKLSPVRDQPSLVLHVDRLSRNVNEAHLKEIFENYGEVVNVELSMDRVVNLPRGYGYVEFKRRADTEKALLYMDGAQIDGNVVKVRFAPAPQQTAAAPSSPKALPHPPKKDVPANDRVGQSTGKATQQRPRESSPQRKLPSSPRRRTPPSRRVDSPRRRPDSPTIRRRTDPSPIRRGETPPSRRPGYPVRRRSPSPLPQRLTSPRRISPRRGRGSPIWRRSPLPPRRMTPPRRMRSPPRRLPPLHRRSRSPIRRPFRSPSRSVSPRRGRGAPVRRGRSSSSSGSLTPPRRGAGRVPRGRSPRRPFRGRSASSYSRSSGSPSPVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HNY7</accession>
    <name>I1HNY7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100844872</name>
        <name evidence="8" type="ORF">BRADI_2g42130v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566822.1">
        <property type="nucleotide sequence ID" value="XM_003566774.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HNY7"/>
    <dbReference type="FunCoup" id="I1HNY7">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK08480">
        <property type="protein sequence ID" value="KQK08480"/>
        <property type="gene ID" value="BRADI_2g42130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844872"/>
    <dbReference type="Gramene" id="KQK08480">
        <property type="protein sequence ID" value="KQK08480"/>
        <property type="gene ID" value="BRADI_2g42130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844872"/>
    <dbReference type="eggNOG" id="KOG0710">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064389_1_0_1"/>
    <dbReference type="OMA" id="GFAQHEV"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43670">
        <property type="entry name" value="HEAT SHOCK PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43670:SF7">
        <property type="entry name" value="OS01G0588000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="7">
        <location>
            <begin position="13"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="98"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="154"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK08480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="283" mass="30704" checksum="3668901CFC71CE04" modified="2012-06-13" version="1">MASKQGSSAAKATAVADIDPTYEWVDGDGIYLLRLNLPGFKKEDFRVHVDPAGRLTIQGHGAGGATRIHKVFQLPSTSDLDGITGRYDGSVLVLTVPKLPPPAVPSPPPPQEQNKQGGQDPAGKAATEQPKSRISREAERLIEAARARLPRTRQAKEQSTAERSEMPTAAKEAEEEKKEEPKPKPKPKPKAEAPSENAKGAAADEHRANVGREAERGIEASRARLQAAAERETTERQEKKGRCWKEEGFKWADAIGNNKEVIATAVAAFTLGVFVSHRLFARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HS01</accession>
    <name>I1HS01_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100834403</name>
        <name evidence="7" type="ORF">BRADI_2g50990v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus lamina</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CRWN family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232388.1">
        <property type="nucleotide sequence ID" value="XM_010234086.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HS01"/>
    <dbReference type="FunCoup" id="I1HS01">
        <property type="interactions" value="1010"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HS01"/>
    <dbReference type="EnsemblPlants" id="KQK09929">
        <property type="protein sequence ID" value="KQK09929"/>
        <property type="gene ID" value="BRADI_2g50990v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834403"/>
    <dbReference type="Gramene" id="KQK09929">
        <property type="protein sequence ID" value="KQK09929"/>
        <property type="gene ID" value="BRADI_2g50990v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834403"/>
    <dbReference type="eggNOG" id="ENOG502QT60">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008819_0_0_1"/>
    <dbReference type="OMA" id="REHKEFM"/>
    <dbReference type="OrthoDB" id="673795at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HS01">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005652">
        <property type="term" value="C:nuclear lamina"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006997">
        <property type="term" value="P:nucleus organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902584">
        <property type="term" value="P:positive regulation of response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000280">
        <property type="term" value="P:regulation of root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040418">
        <property type="entry name" value="CRWN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31908">
        <property type="entry name" value="PROTEIN CROWDED NUCLEI 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31908:SF2">
        <property type="entry name" value="PROTEIN CROWDED NUCLEI 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="950"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="136"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="419"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="965"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK09929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="997" mass="115693" checksum="1FCA6DFDE248F373" modified="2012-06-13" version="1">MASPRSPVGGAAAGDETIWKKLSEAGFDEESVRRRDKAALIAYISRLESEIYDYQHNLGLVLLERKELTLKHEQLRASSESAEIMYKRERASQQSALAEARKREENLKKSLGIQKEFVANLEKALHDMRGETAETKNSYETKLAEALKMMDSAQKKLDEAEEKLFAAKSLEIESTRVHNTALRSLQDLEDREDQLRRYRISNELVYEAKEKDISLQRKSLNDTKKILHDKEQVLLTEQTLLNQRDENILERLTFVTQSEKRLEEDRLILESERMVLMEERNNLVLKMEGIASREEAIIQKETLLDKRESELLIFQETIANKERAEIDRLNQEHEMALERRKLECETEIENKRLAYEAEMEEKITLLDQRERALSEQELAFAQREQNVDLRLAELASMEEALSGRSGQLKVEEGKLLSHRETVHIELQKEREEIQKMKLDLEKEKVFFEEEKQDAIQAQQNLAITQADRDDLLTLQMKLKEEIDNLRAQKKELMADADRLQGEKERFEIEWELIDEKKEELQKEAARISEERRLITEHLKSESDVIKQEKEKLRAQFRNNSETLSREHEEFMSKMQREHASWLSTIQLEREDLTRDIDNQRMELLNSAKAKQMEIDSYLREREEEFEQKKSKELEYINSQKDTINSKLEHAALELQKLEDERKDAALEREKREQELSEIKTTIEALNNQREKLQEQRKLLHSDREAITEQIQQLNVLEELKTDSENKQLCLTECGKSKMNDNGLPPGEDHHATPKNCSSPKLLERKLEVSPSVSTPISWVRKYAQVIFKRSPEKSADHDSDNILHNGLPKNLQKAVDINGSHADQLANGAGEVPQDFDGAKVGKKRHYLVSCDQSDVLEPRRKHQRSTIQKVIRGEITSNCPSVLEEKCSKNEHDAIQLGLSEYGNKGAQNLRLPVDPASSDDLAFANGKADHSGFVDDDETSEEITVCATEQTSGYAIERRDEQDKDVDDEDTDDEEEELEEEKTSSAKKLWRFLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HT11</accession>
    <name>I1HT11_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100824273</name>
        <name evidence="6" type="ORF">BRADI_2g53940v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564528.1">
        <property type="nucleotide sequence ID" value="XM_003564480.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314064.1">
        <property type="nucleotide sequence ID" value="XM_024458296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HT11"/>
    <dbReference type="STRING" id="15368.I1HT11"/>
    <dbReference type="EnsemblPlants" id="KQK10406">
        <property type="protein sequence ID" value="KQK10406"/>
        <property type="gene ID" value="BRADI_2g53940v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73130">
        <property type="protein sequence ID" value="PNT73130"/>
        <property type="gene ID" value="BRADI_2g53940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824273"/>
    <dbReference type="Gramene" id="KQK10406">
        <property type="protein sequence ID" value="KQK10406"/>
        <property type="gene ID" value="BRADI_2g53940v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73130">
        <property type="protein sequence ID" value="PNT73130"/>
        <property type="gene ID" value="BRADI_2g53940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824273"/>
    <dbReference type="eggNOG" id="KOG2602">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014798_2_1_1"/>
    <dbReference type="OMA" id="KHPVARF"/>
    <dbReference type="OrthoDB" id="1724197at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.50:FF:000005">
        <property type="entry name" value="Outer membrane OMP85 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.310:FF:000016">
        <property type="entry name" value="Outer membrane OMP85 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.310">
        <property type="entry name" value="membrane protein fhac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF50">
        <property type="entry name" value="BACTERIAL SURFACE ANTIGEN (D15) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0934">Plastid</keyword>
    <keyword evidence="1" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="5">
        <location>
            <begin position="185"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK10406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="531" mass="57876" checksum="B0E5A82411FF9312" modified="2012-06-13" version="1">MAVTADSTAEQAPNPVPHDPSEESRKAPDQADREEANEGESDEEYDAEELDEPTAEAADREAVQAVFRRLSSEPVSIRVHDVIIMGNSKTRDTLIEAEVADLLRSASTVQDLVRASSLASGRLRRLQVFDSVTITLDAGPPELPGTTNVVIEVVEAASPIAADIGCFSKPEARSWSLEGSLKLKNLFGYGDIWDASGAYGWDQSSEVAVGVSLPRFKSVPTPLTARASLLSQDWLKFSSYKERLLGLSFGLLSTRHHDLSYNLTWQTLTDPSQMASKSIRRQLGHNLLSALTYTHKIDQRDSQLRPTKGYAFVSSSHIGGLWDMKGLKFFRQEFDVRAAVPFGFYNAALNIGVAAGVILPLGKGFMELPSPVTNRFFLGGHSSPVCSLGALSSLLGFKMRGVGPSEPRRFAPSESVMDDSATSPGRDYLGGDLAVSAFADLSFDLPLKLFRDAGIYGHAFLSAGNLAKLSESEFKNFSFPEFGRTFRSSAGVGLVLPTKLLRVEINYCYILKKFEHDRGKTGIQFSFSSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HW62</accession>
    <name>I1HW62_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UFSP1/2/DUB catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100824606</name>
        <name evidence="4" type="ORF">BRADI_3g00940v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233670.1">
        <property type="nucleotide sequence ID" value="XM_010235368.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233671.1">
        <property type="nucleotide sequence ID" value="XM_010235369.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316847.1">
        <property type="nucleotide sequence ID" value="XM_024461079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HW62"/>
    <dbReference type="STRING" id="15368.I1HW62"/>
    <dbReference type="EnsemblPlants" id="KQJ92823">
        <property type="protein sequence ID" value="KQJ92823"/>
        <property type="gene ID" value="BRADI_3g00940v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92824">
        <property type="protein sequence ID" value="KQJ92824"/>
        <property type="gene ID" value="BRADI_3g00940v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92825">
        <property type="protein sequence ID" value="KQJ92825"/>
        <property type="gene ID" value="BRADI_3g00940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824606"/>
    <dbReference type="Gramene" id="KQJ92823">
        <property type="protein sequence ID" value="KQJ92823"/>
        <property type="gene ID" value="BRADI_3g00940v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92824">
        <property type="protein sequence ID" value="KQJ92824"/>
        <property type="gene ID" value="BRADI_3g00940v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92825">
        <property type="protein sequence ID" value="KQJ92825"/>
        <property type="gene ID" value="BRADI_3g00940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824606"/>
    <dbReference type="eggNOG" id="KOG4696">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031428_1_0_1"/>
    <dbReference type="OMA" id="HANAHFD"/>
    <dbReference type="OrthoDB" id="288987at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019783">
        <property type="term" value="F:ubiquitin-like protein peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012462">
        <property type="entry name" value="UFSP1/2_DUB_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48153:SF4">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MUG105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48153">
        <property type="entry name" value="UFM1-SPECIFIC PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07910">
        <property type="entry name" value="Peptidase_C78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UFSP1/2/DUB catalytic" evidence="3">
        <location>
            <begin position="127"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ92824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="405" mass="45457" checksum="1D162D5FDE5B402E" modified="2012-06-13" version="1">MISSCPICDKQVLTAELEWHANSHFEDDEAQRDMELAQLAAVTELSTSVTDVPQHSMRSFSNDSFTQGASSSTIRGSSPYGNVLDEQISCLIGAQIRSTVQKIEGGVMNLLRSCLESEGGSSTSIISGYVEHHQSLSSEDKGWGCGWRNIQMLSSHLLKQRPEAREVLFGGHGFVPDIPSLQRWLEIAWDKGFDTVGSSHFHNKVYGAKTWIGTTECVTLLCSFGLRARIVDFDSTESSGQQTKSGKRVCGPMDKYLVKTNSSCELSQEDAENMRGQQVLVDWVWNYFTSKRSDILHNSKNVTISDKTPLYFQHQGHSRTIVGIQRQKGYRGSQDQYNLLVLDPGHRTADLERSLTIKKGWQRLVKRGVHTLRKPQYQLCYVDPGIASLEEMEQLKTIDSILIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I1HY62</accession>
    <name>I1HY62_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC104583957</name>
        <name evidence="2" type="ORF">BRADI_3g06610v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236304.1">
        <property type="nucleotide sequence ID" value="XM_010238002.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HY62"/>
    <dbReference type="EnsemblPlants" id="KQJ93770">
        <property type="protein sequence ID" value="KQJ93770"/>
        <property type="gene ID" value="BRADI_3g06610v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583957"/>
    <dbReference type="Gramene" id="KQJ93770">
        <property type="protein sequence ID" value="KQJ93770"/>
        <property type="gene ID" value="BRADI_3g06610v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583957"/>
    <dbReference type="eggNOG" id="ENOG502R65I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2149458_0_0_1"/>
    <dbReference type="OMA" id="MQPANCV"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094878" evidence="1">
        <location>
            <begin position="28"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ93770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="102" mass="11067" checksum="10B3D68A10BF9150" modified="2012-06-13" version="1">MQPANCVSLLLLLAVLLLAALGPFAAAAHRSELVMAAAGDRMETTAAAEKGSEESATTYRRSLLAVHFRTRKVPSDPKNWLDGRVAFTADYHAVQRHPSKHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYA9</accession>
    <name>I1HYA9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Lipoxygenase</fullName>
            <ecNumber evidence="20">1.13.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC100832776</name>
        <name evidence="24" type="ORF">BRADI_3g07000v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">Bd21</strain>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(9Z,12Z)-octadecadienoate + O2 = (13S)-hydroperoxy-(9Z,11E)-octadecadienoate</text>
            <dbReference type="Rhea" id="RHEA:22780"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:57466"/>
            <dbReference type="EC" id="1.13.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(9Z,12Z,15Z)-octadecatrienoate + O2 = (13S)-hydroperoxy-(9Z,11E,15Z)-octadecatrienoate</text>
            <dbReference type="Rhea" id="RHEA:34495"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32387"/>
            <dbReference type="ChEBI" id="CHEBI:58757"/>
            <dbReference type="EC" id="1.13.11.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="20">Lipid metabolism; oxylipin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.-" evidence="20"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571227.1">
        <property type="nucleotide sequence ID" value="XM_003571179.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYA9"/>
    <dbReference type="STRING" id="15368.I1HYA9"/>
    <dbReference type="EnsemblPlants" id="KQJ93838">
        <property type="protein sequence ID" value="KQJ93838"/>
        <property type="gene ID" value="BRADI_3g07000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832776"/>
    <dbReference type="Gramene" id="KQJ93838">
        <property type="protein sequence ID" value="KQJ93838"/>
        <property type="gene ID" value="BRADI_3g07000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832776"/>
    <dbReference type="eggNOG" id="ENOG502QQSP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004282_0_0_1"/>
    <dbReference type="OMA" id="MEMPEHV"/>
    <dbReference type="OrthoDB" id="407298at2759"/>
    <dbReference type="UniPathway" id="UPA00382"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016165">
        <property type="term" value="F:linoleate 13S-lipoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010597">
        <property type="term" value="P:green leaf volatile biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009753">
        <property type="term" value="P:response to jasmonic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000021">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.60:FF:000005">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.372.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.375.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.375.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.372.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001246">
        <property type="entry name" value="LipOase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027433">
        <property type="entry name" value="Lipoxygenase_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00468">
        <property type="entry name" value="PLTLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11 19" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="15 20" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13 19" id="KW-0408">Iron</keyword>
    <keyword evidence="4 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 20" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="PLAT" evidence="22">
        <location>
            <begin position="80"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="23">
        <location>
            <begin position="202"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQJ93838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="897" mass="100892" checksum="94FFEFB0E3E2A860" modified="2012-06-13" version="1">MIHLKQPLTLSAQSGNVGSTPLFATAAATSSCQKIRRGARRRAAARRTIRCASTEQAVGVSTVTTERALTVTATVTAQAPIGVVYVSRGIDDLQDLLGKTLLLELVSSELDPKTGMERERVKGFAHMTVTDGVYEAKMSVPASFGPVGAVVVENEHHKEMFIKDIKLVTGGDESSAVAFDVASWVHSKFDNPEPRVFFTVKSYLPSQTPPGIEALRRKELETLRGDGTGERKFHERVYDYDTYNDLGDPDKNIEHLRPVLGGKEHPYPRRCRTGRPKTLCSPETEKRSSPVYVPRDEQFSDVKGRTFSATTLRTGLHAILPALSPLINNSRCFSHFPAIDALYSDGIALPVDVAGTSIDAIGGVIPRVVRMIEDTTEHVLRFEVPQMVERDRFSWFRDEEFARQTLAGLNPICIRLLTEFPIMSKLDPAVYGPAESALSKELLEKMMNGTNAMTVEEALEAKRLFILDYHDVFLPYVHKVRELPDTTLYGSRTIFLLTDEGTLMPLAIELTRPQSPTKPQWKRAFTHGPDATESWLWKIAKAHVLTHDTGYHQLVSHWLRTHASVEPYIIAANRQLSRMHPVYRLLHPHFRYTMEINALARESLINADGIIEEAFWPGKYSIELSSVAYAATWRFDTEALPEDLVTRGLAVRGDDGELELTIKDYPYANDGLLIWNSIKQWVSDYVKFYYKSDEDVTGDKEVQAWWEEVRTKGHADKKDEPWWPVCDSRDNLVQILTIIMWVTSGHHAAVNFGQYHYGGYFPNRPTVVRKNIPVEENKEDEMRKFMARPEEVLLQSLPSQMQAIKVMATLDILSSHSPDEEYMGEYAEPAWLAEPMVKAAFEKFSGRLKEVEGTIDERNNNTDNKNRCGAGIVPYELLKPFSGPGVTGRGIPNSISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1I493</accession>
    <name>I1I493_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein preY, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100837591</name>
        <name evidence="4" type="ORF">BRADI_3g27420v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PREY family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573964.1">
        <property type="nucleotide sequence ID" value="XM_003573916.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I493"/>
    <dbReference type="STRING" id="15368.I1I493"/>
    <dbReference type="EnsemblPlants" id="KQJ96852">
        <property type="protein sequence ID" value="KQJ96852"/>
        <property type="gene ID" value="BRADI_3g27420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837591"/>
    <dbReference type="Gramene" id="KQJ96852">
        <property type="protein sequence ID" value="KQJ96852"/>
        <property type="gene ID" value="BRADI_3g27420v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837591"/>
    <dbReference type="eggNOG" id="ENOG502S9UK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187192_0_0_1"/>
    <dbReference type="OMA" id="TDGHKEM"/>
    <dbReference type="OrthoDB" id="1884515at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000023">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33505:SF4">
        <property type="entry name" value="PROTEIN PREY, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33505">
        <property type="entry name" value="ZGC:162634"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ96852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="88" mass="9235" checksum="47B9C60FE2423391" modified="2012-06-13" version="1">MRRTAALLLRHGGGGGGISQALADMLVCPLTKKPLRYCEASGSLVSDAVGISFPIVDGIPSLLPKDGKLLEDHQEKSGHESSPRDSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1I7U6</accession>
    <name>I1I7U6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc finger ZPR1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100836574</name>
        <name evidence="10" type="ORF">BRADI_3g38220v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574629.1">
        <property type="nucleotide sequence ID" value="XM_003574581.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I7U6"/>
    <dbReference type="FunCoup" id="I1I7U6">
        <property type="interactions" value="2354"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I7U6"/>
    <dbReference type="EnsemblPlants" id="KQJ98646">
        <property type="protein sequence ID" value="KQJ98646"/>
        <property type="gene ID" value="BRADI_3g38220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836574"/>
    <dbReference type="Gramene" id="KQJ98646">
        <property type="protein sequence ID" value="KQJ98646"/>
        <property type="gene ID" value="BRADI_3g38220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836574"/>
    <dbReference type="eggNOG" id="KOG2703">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024138_0_0_1"/>
    <dbReference type="OMA" id="KCTFDQN"/>
    <dbReference type="OrthoDB" id="308464at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I7U6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000002">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000002">
        <property type="entry name" value="zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="entry name" value="ZPR1, zinc finger domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="Zn_ribbon_ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="9">
        <location>
            <begin position="36"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="9">
        <location>
            <begin position="291"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ98646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="496" mass="54112" checksum="A491F258725F44D5" modified="2012-06-13" version="1">MASSGEEGRVVVDLRSAAESAGDEDAHATPLHEIESLCMRCGENGITRILLTLIPHFREVVLMAFECPHCSERNNEVQFAGQLQPKGCCYRLEVPSGKSEILNRQVVKSDSATIKIPELDFEIPPEAQRGTLSTVEGCIMRAVDELQALQDERKKVDPQKAEAIDKFLVKLKSLGSGEVAFTFILDDPSGNSFIENPHAPSSDPLLSLKFYERTSEQQAALGFLAEPTKEPGESSRDASTVEAKSGGLQRMPHGSVGAIAGRRAIAQGNSDEVTAALCRYSAPEEVDTLPSTCGACATECVTRFFATKIPYFREVIVMATTCDVCGYRNSELKPGGEIPAKGKKTTLRVQNAKDLSRDVIKSDSAAVQVPELDLELSSGTLGGMVTTVEGLIVKICEALERVHGFQLGDSTHEWKKKKWDGFTERLSKLLSLQEPWTLIIDDALAASFVAPATDSIEDDHQLTIEEYERSWEQNEELGLNDMDTSSADMAYNTTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8X3</accession>
    <name>I1I8X3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MADS-box transcription factor 26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100844294</name>
        <name evidence="10" type="ORF">BRADI_3g41260v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2014" name="PLoS ONE" volume="9" first="e84781" last="e84781">
            <title>Genome-wide analysis of the MADS-box gene family in Brachypodium distachyon.</title>
            <authorList>
                <person name="Wei B."/>
                <person name="Zhang R.Z."/>
                <person name="Guo J.J."/>
                <person name="Liu D.M."/>
                <person name="Li A.L."/>
                <person name="Fan R.C."/>
                <person name="Mao L."/>
                <person name="Zhang X.Q."/>
            </authorList>
            <dbReference type="PubMed" id="24454749"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KF469321">
        <property type="protein sequence ID" value="AIG21835.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001288311.1">
        <property type="nucleotide sequence ID" value="NM_001301382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8X3"/>
    <dbReference type="FunCoup" id="I1I8X3">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99121">
        <property type="protein sequence ID" value="KQJ99121"/>
        <property type="gene ID" value="BRADI_3g41260v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844294"/>
    <dbReference type="Gramene" id="KQJ99121">
        <property type="protein sequence ID" value="KQJ99121"/>
        <property type="gene ID" value="BRADI_3g41260v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844294"/>
    <dbReference type="eggNOG" id="KOG0014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053053_0_2_1"/>
    <dbReference type="OMA" id="PAWDNIG"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000011">
        <property type="entry name" value="MADS-box transcription factor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="90"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AIG21835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ99121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="250" mass="28916" checksum="EE2CB80EDC2AA2C0" modified="2012-06-13" version="1">MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIVFSNRGKLYEFCSTQSMTKTLEKYQKCSYAGPETAVQNRENEQLKNSRNEYLKLKARVDNLQRTQRNLLGEDLESLGIKELEGLEKQLDSSLKHIRTTRTQHMVDQLTELQRREQMFSEANKCLRIKLEESNQVHGQQLWEHNNNLLSYERQPEVQPQMHGGNGFFHPLDAAGEPTLHIGYPSETMNSGMNSSCMTTFMPPWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBT2</accession>
    <name>I1IBT2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">gibberellin 2beta-dioxygenase</fullName>
            <ecNumber evidence="8">1.14.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100844598</name>
        <name evidence="11" type="ORF">BRADI_3g49390v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>gibberellin A1 + 2-oxoglutarate + O2 = gibberellin A8 + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15005"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58524"/>
            <dbReference type="ChEBI" id="CHEBI:58594"/>
            <dbReference type="EC" id="1.14.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.13" evidence="8"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575413.1">
        <property type="nucleotide sequence ID" value="XM_003575365.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBT2"/>
    <dbReference type="STRING" id="15368.I1IBT2"/>
    <dbReference type="EnsemblPlants" id="KQK00433">
        <property type="protein sequence ID" value="KQK00433"/>
        <property type="gene ID" value="BRADI_3g49390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844598"/>
    <dbReference type="Gramene" id="KQK00433">
        <property type="protein sequence ID" value="KQK00433"/>
        <property type="gene ID" value="BRADI_3g49390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844598"/>
    <dbReference type="eggNOG" id="KOG0143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010119_15_1_1"/>
    <dbReference type="OMA" id="CEEPSLY"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045543">
        <property type="term" value="F:gibberellin 2-beta-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045487">
        <property type="term" value="P:gibberellin catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000021">
        <property type="entry name" value="Gibberellin 2-beta-dioxygenase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="10">
        <location>
            <begin position="212"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK00433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="356" mass="38894" checksum="44388AD6F53ABA13" modified="2012-06-13" version="1">MPAFAESAAEPPLADSYHALLRRAGADGRNKDAHCTPTVHADHVLPAPDMAVSECELPMIDVGCLTTGGGSAEERAACAAAIAGAAEDWGFFQVVNHGVKQELLEAMRREQTRLFRLPFEAKATAGLLNHSYRWGTPTATSPAQLSWSEAFHVPLAAVSGDPPCNYGQLTTLRDVTQEVATAMSKLANTLARVLAERLGHAGERFPEGCDERTCFLRLNRYPPCPLSPDAFGLVPHTDSDFLTVLCQDQVGGLQLMKGSRWLAVKPIPNALIVNIGDLFQAWSNNRYRSVEHKVVTNATTERYSVAYFLCPSYDSPIGACEEPSPYRTFTFGEYRRRVQEDVKKTGKKVGLPGFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICY7</accession>
    <name>I1ICY7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PRONE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100844470</name>
        <name evidence="4" type="ORF">BRADI_3g52620v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK00915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK00916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570179.1">
        <property type="nucleotide sequence ID" value="XM_003570131.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235857.1">
        <property type="nucleotide sequence ID" value="XM_010237555.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756826.1">
        <property type="nucleotide sequence ID" value="XM_014901340.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_014901341.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756829.1">
        <property type="nucleotide sequence ID" value="XM_014901343.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICY7"/>
    <dbReference type="STRING" id="15368.I1ICY7"/>
    <dbReference type="EnsemblPlants" id="KQK00913">
        <property type="protein sequence ID" value="KQK00913"/>
        <property type="gene ID" value="BRADI_3g52620v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00914">
        <property type="protein sequence ID" value="KQK00914"/>
        <property type="gene ID" value="BRADI_3g52620v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00915">
        <property type="protein sequence ID" value="KQK00915"/>
        <property type="gene ID" value="BRADI_3g52620v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00916">
        <property type="protein sequence ID" value="KQK00916"/>
        <property type="gene ID" value="BRADI_3g52620v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69280">
        <property type="protein sequence ID" value="PNT69280"/>
        <property type="gene ID" value="BRADI_3g52620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844470"/>
    <dbReference type="Gramene" id="KQK00913">
        <property type="protein sequence ID" value="KQK00913"/>
        <property type="gene ID" value="BRADI_3g52620v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00914">
        <property type="protein sequence ID" value="KQK00914"/>
        <property type="gene ID" value="BRADI_3g52620v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00915">
        <property type="protein sequence ID" value="KQK00915"/>
        <property type="gene ID" value="BRADI_3g52620v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00916">
        <property type="protein sequence ID" value="KQK00916"/>
        <property type="gene ID" value="BRADI_3g52620v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69280">
        <property type="protein sequence ID" value="PNT69280"/>
        <property type="gene ID" value="BRADI_3g52620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844470"/>
    <dbReference type="eggNOG" id="ENOG502QPJS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019073_3_0_1"/>
    <dbReference type="OMA" id="WNFMKDS"/>
    <dbReference type="OrthoDB" id="1053009at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2010:FF:000001">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2010:FF:000003">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2010">
        <property type="entry name" value="PRONE domain, subdomain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005512">
        <property type="entry name" value="PRONE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038937">
        <property type="entry name" value="RopGEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101">
        <property type="entry name" value="ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101:SF2">
        <property type="entry name" value="ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03759">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRONE" evidence="3">
        <location>
            <begin position="116"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK00916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="543" mass="60797" checksum="CEB2E0373E2C09F0" modified="2012-06-13" version="1">MRMKTLACCRRRPQDFSIDMDQEPDRVTTYNGLERCIFNSSSYDEDSGISATTGVDGCVTTDSLDDEVSSCSSSKDVDGSSFSSQYISSSKQEEHSVYGLDTLDTVHQLPVKGKNPIRYTLSASDIETMKEKFAKLLLGDDISGGARGVCAALALSNGITNLSATIFGELWKLEPLCEEKKIRWRKEMDWLLSPTTYMVELVPTKQNGADGCIFEIMTPKARSDVHVNLPALQKLDSMLIEVLDSMVDTEYWYVESGSRAGDRGKNNGPRQTKKWWLPSPCVPEQGLSQFQRKRIIFQAKLVHQILKAAKSINEQVLLQIPIPTAVMGALPKSGRASLGEDLYHAITTDYIPIEDIFISLSLKTEHSVLETMNRLEGALFAWNQRISEERSKKSPGRHSWNFMKDSSSEVEKMTACIERVETLMQLLKSRFPNLPPTFTDVVKVQYNEDVGHAIVEAYSRVLVGVSFSILSRIAEVMLEDDLIKKPNTPMATLKFDLSSDVYLAGITETPPGHIRRSLMDQISMVDGRFDTVVKKKGAKQLMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1ID85</accession>
    <name>I1ID85_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-haem dioxygenase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100837486</name>
        <name evidence="4" type="ORF">BRADI_3g53430v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570241.1">
        <property type="nucleotide sequence ID" value="XM_003570193.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ID85"/>
    <dbReference type="EnsemblPlants" id="KQK01036">
        <property type="protein sequence ID" value="KQK01036"/>
        <property type="gene ID" value="BRADI_3g53430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837486"/>
    <dbReference type="Gramene" id="KQK01036">
        <property type="protein sequence ID" value="KQK01036"/>
        <property type="gene ID" value="BRADI_3g53430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837486"/>
    <dbReference type="eggNOG" id="ENOG502QR0G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047601_0_0_1"/>
    <dbReference type="OMA" id="HQPDPKC"/>
    <dbReference type="OrthoDB" id="10248513at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000019">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF874">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK01036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="39336" checksum="6222C46ABB7AC0B6" modified="2012-06-13" version="1">MDLPVVDLAPYLQIAGGTGPAEAEEGIRALCAAVSASLRDTGALLVKDPRCSAADNDRFLDVVERYFARSDDSKRLQERPQLHYQVGVTPEGVEVPRSLVDKEMQDKIKSMPDEFQPATPKGPDPKWRYMWRVGPRPSNTRFKELNSEPVIPDGLPEWKETMDSWGSKMISAIEVVAEMAAIGFGLSKDAFTSLMKEGPHLLAPTGSDLLRHGSEGTVFAGFHYDLNFLTIHGRSRFPGLNIWLRNGKKMEVKVPVGCLLIQSGKQLEWLTGGECLAGMHEVVVTKRTIEAIALAKEQNRSLWRVSSTLFSHIASDATIKPLGHFAEAPNANSYPPICAGDYVEQELAAINLKGKNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1IE49</accession>
    <name>I1IE49_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100842861</name>
        <name evidence="3" type="ORF">BRADI_3g55900v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570430.2">
        <property type="nucleotide sequence ID" value="XM_003570382.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IE49"/>
    <dbReference type="STRING" id="15368.I1IE49"/>
    <dbReference type="EnsemblPlants" id="KQK01447">
        <property type="protein sequence ID" value="KQK01447"/>
        <property type="gene ID" value="BRADI_3g55900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842861"/>
    <dbReference type="Gramene" id="KQK01447">
        <property type="protein sequence ID" value="KQK01447"/>
        <property type="gene ID" value="BRADI_3g55900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842861"/>
    <dbReference type="eggNOG" id="ENOG502S79F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154107_0_0_1"/>
    <dbReference type="OrthoDB" id="747994at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IE49">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37756">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37756:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK01447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15098" checksum="72E144EA6856A2C9" modified="2012-06-13" version="1">MPTRCLSSSSPSSSPSQTKSSQNRDDEPIQGEAEQQGDKPHLKEAMVCVACLIPIFLIPFVNALPFLFDLLLSKIYRMFGWEYRRPERAPPACPFKPAANKTEGATGESKPLVNPHVASESKPLVEPQGASTEGKKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1IG67</accession>
    <name>I1IG67_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100844497</name>
        <name evidence="7" type="ORF">BRADI_4g00950v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Probable oxidoreductase that may play a role as regulator of mitochondrial function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with COX5B; this interaction may contribute to localize PYROXD2 to the inner face of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carotenoid/retinoid oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576314.2">
        <property type="nucleotide sequence ID" value="XM_003576266.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IG67"/>
    <dbReference type="FunCoup" id="I1IG67">
        <property type="interactions" value="265"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IG67"/>
    <dbReference type="EnsemblPlants" id="KQJ85676">
        <property type="protein sequence ID" value="KQJ85676"/>
        <property type="gene ID" value="BRADI_4g00950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844497"/>
    <dbReference type="Gramene" id="KQJ85676">
        <property type="protein sequence ID" value="KQJ85676"/>
        <property type="gene ID" value="BRADI_4g00950v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844497"/>
    <dbReference type="eggNOG" id="KOG4254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019327_0_1_1"/>
    <dbReference type="OMA" id="GLYHCGS"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IG67">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668">
        <property type="entry name" value="PHYTOENE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668:SF103">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="6">
        <location>
            <begin position="45"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ85676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="579" mass="62161" checksum="8A4FC267B1A74B05" modified="2012-06-13" version="1">MPMNRVLLAGVRRLSTSASPSSAAASQLQGGKRWDAVVVGGGHNGLAAAAYLARAGRSVAVLERRGVLGGAAVSESGLVPGFRFSRCSYLLSLLRPSLIQELELERHGLKLLPRSPSSFTPCLDGRYLLLGPDAELNHSEIGKFSKKDAEAYPRYEEQLEKYCKLMDFVIDSPPPELRQLYHASMVDRMKDKVDKSVFWSKLVGIVMQQGQKDMVNFFDLLLSPASKVLNNWFEGEVLKATLATDAVIGTMAGVNTPGSGYVLLHHIMGETGGQRGVWAYVQGGMGSVSSAISKAALEAGVQIVTNAEVSQIMVDETTGKVQGVGLADGTELHSSVVLSNATPYKTFVDLVPANILPEEFLCAIKTADYSSATTKINVAVDRLPQFNCCKGINPEGGPEHMGTIHIGSESMEEIDLAYKEATGGVSSTRPVIEMTIPSVLDKTISPPGQHVINLFVQYTPYKLSEGSWQDSTVRKSFAERCFSLIDDYAPGFSSSVIGYDMLTPPDLEREFGLTGGNIFHGAMGLDSLFLMRPAKGWSDYRTPVKGLYLCGSGAHPGGGVMGAPGRNAASVVLDDLKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1IHA4</accession>
    <name>I1IHA4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100839565</name>
        <name evidence="2" type="ORF">BRADI_4g04020v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579271.3">
        <property type="nucleotide sequence ID" value="XM_003579223.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IHA4"/>
    <dbReference type="STRING" id="15368.I1IHA4"/>
    <dbReference type="EnsemblPlants" id="KQJ86212">
        <property type="protein sequence ID" value="KQJ86212"/>
        <property type="gene ID" value="BRADI_4g04020v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839565"/>
    <dbReference type="Gramene" id="KQJ86212">
        <property type="protein sequence ID" value="KQJ86212"/>
        <property type="gene ID" value="BRADI_4g04020v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839565"/>
    <dbReference type="eggNOG" id="ENOG502S4CS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1951749_0_0_1"/>
    <dbReference type="OMA" id="DYPREAM"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34658:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34658">
        <property type="entry name" value="OS01G0151800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ86212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="129" mass="13127" checksum="76E20D78DFFE5146" modified="2012-06-13" version="1">MHPLMGALLEAGDGRRWSRCFFSFFLGPVARQAAAWAGALALAVSVASFAPEAAFVWALSGGGGGGCAAGAVRVPLDGGGDHLCVPARMAGRTCADLLVPPAFAALSVAASACFVRALAIGRRRDGYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1II30</accession>
    <name>I1II30_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100844490</name>
        <name evidence="12" type="ORF">BRADI_4g06410v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575479.1">
        <property type="nucleotide sequence ID" value="XM_003575431.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311044.1">
        <property type="nucleotide sequence ID" value="XM_024455276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1II30"/>
    <dbReference type="EnsemblPlants" id="KQJ86575">
        <property type="protein sequence ID" value="KQJ86575"/>
        <property type="gene ID" value="BRADI_4g06410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844490"/>
    <dbReference type="Gramene" id="KQJ86575">
        <property type="protein sequence ID" value="KQJ86575"/>
        <property type="gene ID" value="BRADI_4g06410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844490"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="OMA" id="WMDYGTS"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000025">
        <property type="entry name" value="probable polyol transporter 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF49">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="30"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ86575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="482" mass="50290" checksum="237204B9143F595F" modified="2012-06-13" version="1">MDAAEEPLLAAASTKPDGAPPPRNKYPFFCAVLASMTSVLTGYNVAVMSGAQIFIAEDLGVSDTQIELLSGAINIYSLVGALLAGWTSDRLGRRLTIVLTNAFFLLGPLTMSLAAGYPALMAGRFVSGVGVGYALVIAPVYAAEISPASSRGLLTSLPEIFINTGVMLSYVSNLVFSGLPAHLSWRLMFAAGVVPTVFLAAGVLTMPESPRWLAMKGRTEEARAVLGRTSDTPAEAQQRLLEIQEVLIVSVPGSGNGNGNNSAWKEAASKAGVRRVLATVLALQFFQQASGIDSVVLYGPRVLAMAGVTSNALLLGLNVLFGVAKAGSILIAMALADRVGRRPLLLVSTGGMTLSLLVLGSVFAAFAGVKDDAAVAAVAVVAVVAFVCTFSVGLGPLAWVYSSEILPLRLRGQGAGLGTAMNRVVSGLVTMTFISLYGAITMAGTFYLYGAVAAASFVFIYTCLPETRGRNLEDMEQLFRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1IIX3</accession>
    <name>I1IIX3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FRIGIDA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100844612</name>
        <name evidence="7" type="ORF">BRADI_4g08780v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Frigida family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577079.1">
        <property type="nucleotide sequence ID" value="XM_003577031.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IIX3"/>
    <dbReference type="STRING" id="15368.I1IIX3"/>
    <dbReference type="EnsemblPlants" id="KQJ86973">
        <property type="protein sequence ID" value="KQJ86973"/>
        <property type="gene ID" value="BRADI_4g08780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844612"/>
    <dbReference type="Gramene" id="KQJ86973">
        <property type="protein sequence ID" value="KQJ86973"/>
        <property type="gene ID" value="BRADI_4g08780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844612"/>
    <dbReference type="eggNOG" id="ENOG502QWA2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026883_2_1_1"/>
    <dbReference type="OMA" id="FYASRMT"/>
    <dbReference type="OrthoDB" id="1930990at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012474">
        <property type="entry name" value="Frigida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31791:SF4">
        <property type="entry name" value="FRIGIDA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31791">
        <property type="entry name" value="FRIGIDA-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07899">
        <property type="entry name" value="Frigida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2 4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="3 4" id="KW-0287">Flowering</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="11"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ86973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="538" mass="60955" checksum="BE80F56D9C974DB5" modified="2012-06-13" version="1">MSDMESVATLMESTSLKIQQLQRAFAELESQSAVSMNFKWKQLEDHFHGLEQSLKKKFDELNKQEKEFQETVAKSEQILEQQEAVVVGKELTSLERLQEKRDAALAVIFGKSKLSLAAPVINQMNKPLSNYSPTLAVKWSKHCPENNVHMQDSSASLKPRSELAILCEEMNVKGLHKFISDNRKNLTSIREEIPSALKRASHPYVLVLDSLEYFYYGDNLVLDGKKDGDLLGVRRTCLMLMESLVQLQADAVTGLLSEEQMCTPNVKERAKRIAFEWKSKLDSLDVDASNGNCLEAHAFLQLLATFGIFAEFNEDELCKLLPSVSRRRQTPELCRLLGLSQKMPGVIGVLVDSAKPIDAINLAYVFGLTEQFEPVQLLKAYLREVRKVSHAKNGKMSPGAQNEMNERELSALKAVIKCIEEHKLEEQYPVDPLQKRVIQLEKAKADKRRAVEAAKPQSKRPRANGSVYAPRVTSFGDKNIYQATPERHPYPYERQFVYSAEAHHHPTMINAAPYTMTPAHTPYYGNGYPMQFQVPYIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMA7</accession>
    <name>I1IMA7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Non-lysosomal glucosylceramidase</fullName>
            <shortName evidence="1">NLGase</shortName>
            <ecNumber evidence="1">3.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100824639</name>
        <name evidence="5" type="ORF">BRADI_4g21277v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:13269"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="3.2.1.45"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the non-lysosomal glucosylceramidase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.45" evidence="1"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237798.1">
        <property type="nucleotide sequence ID" value="XM_010239496.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758507.1">
        <property type="nucleotide sequence ID" value="XM_014903021.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IMA7"/>
    <dbReference type="STRING" id="15368.I1IMA7"/>
    <dbReference type="EnsemblPlants" id="KQJ88807">
        <property type="protein sequence ID" value="KQJ88807"/>
        <property type="gene ID" value="BRADI_4g21277v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ88808">
        <property type="protein sequence ID" value="KQJ88808"/>
        <property type="gene ID" value="BRADI_4g21277v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ88809">
        <property type="protein sequence ID" value="KQJ88809"/>
        <property type="gene ID" value="BRADI_4g21277v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824639"/>
    <dbReference type="Gramene" id="KQJ88807">
        <property type="protein sequence ID" value="KQJ88807"/>
        <property type="gene ID" value="BRADI_4g21277v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88808">
        <property type="protein sequence ID" value="KQJ88808"/>
        <property type="gene ID" value="BRADI_4g21277v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88809">
        <property type="protein sequence ID" value="KQJ88809"/>
        <property type="gene ID" value="BRADI_4g21277v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824639"/>
    <dbReference type="eggNOG" id="KOG2119">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006322_1_0_1"/>
    <dbReference type="OMA" id="HDLGAPN"/>
    <dbReference type="OrthoDB" id="730489at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004348">
        <property type="term" value="F:glucosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006680">
        <property type="term" value="P:glucosylceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000006">
        <property type="entry name" value="Non-lysosomal glucosylceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014551">
        <property type="entry name" value="B_Glucosidase_GBA2-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006775">
        <property type="entry name" value="GH116_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024462">
        <property type="entry name" value="GH116_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052566">
        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654">
        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654:SF31">
        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04685">
        <property type="entry name" value="DUF608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12215">
        <property type="entry name" value="Glyco_hydr_116N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028944">
        <property type="entry name" value="Beta_gluc_GBA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl-hydrolase family 116 N-terminal" evidence="4">
        <location>
            <begin position="99"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="3">
        <location>
            <begin position="532"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR028944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ88807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="953" mass="106247" checksum="CD24BB01F5112D1B" modified="2012-06-13" version="1">MKPNGMPEPPKGVSRNSSPSQTNGDSEKVDPGQLPELTWEHKLSHVRYDLPSFGLKWREAVKMAGLGFRLGQHIVEETSKGRTAIIDPMKKRTAKSGQGVPLGGIGAGSIGRSYKGEFQRWQLFPGACEDKPVLANQFSAFISRQDGRKYSTVLHPGKPDLPKGTNISGIGSWDWNLSGQKSTYHALYPRAWTVYDGEPDPDLKIVCRQVSPIIPHNYQQSSYPAAVFTFTVANSGNTAVDVTLLFTWANSVGGKSELTGYHCNSSMLEKDGVHGILLHHRTADGQPPVTFAIAAQEKEDVHISECPYFVMSGSSDEFTAKDMWNSVKEHGSFDLLDPIEASICSRPGTSIGAAIAASVKLAPQSTKDVSFSLAWACPEVKFSSGKTYHRRYTKFYGTDVDAAASLAHDAIVDHSSWEKQIEEWQNPILQDKRFPAWYPVTLFNELYYLNAGGSIWTDGLPPIQSLTAIGGKKFSLDMSNGETDDVDEMIPHNNTATDILQQMASILERIHASLASNSAIGTTLLQGEENIGQFLYLEGIEYYMWNTYDVHFYASFALIMLFPKLQLSIQRDFAAAVMMHDPEKLKLLHDGKLAPRKVLGAVPHDLGLYDPWFKVNAYTLHNTDRWKDLNPKFVLQVYRDVVATGNKSFARAVWPSVYMAMAYMEQFDKDKDGMIENEDFPDQTYDVWSMAGVSAYCGGLWVAALQAASALAREVGDKASEELFWNKYEKAKSVYGKLWNGSYFNYDDAGTKVSTSIHADQLAGQWYAKSCGLSSIVDKDKSQSALEKIYSFNVMKFKDGKRGAMNGMWPDGTVDMSTMQSREIWPGVTYALAASMIQEGMVEEGFKTAEGIYHAAWSPEGLGYAFQTPEAWTNDDGYRSLCYMRPLAIWSIQWALSSPKLHKEPQRDLAQDSFPKNQFSYARIAKLLQLPEDESSKSFLQVIYEIVRNRFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPL3</accession>
    <name>I1IPL3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminopeptidase N-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g28760v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPL3"/>
    <dbReference type="STRING" id="15368.I1IPL3"/>
    <dbReference type="EnsemblPlants" id="KQJ89966">
        <property type="protein sequence ID" value="KQJ89966"/>
        <property type="gene ID" value="BRADI_4g28760v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89966">
        <property type="protein sequence ID" value="KQJ89966"/>
        <property type="gene ID" value="BRADI_4g28760v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1117040_0_0_1"/>
    <dbReference type="InParanoid" id="I1IPL3"/>
    <dbReference type="OrthoDB" id="694231at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF203">
        <property type="entry name" value="AMINOPEPTIDASE M1-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="3">
        <location>
            <begin position="136"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ89966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="249" mass="26653" checksum="E0D214335F946CBC" modified="2012-06-13" version="1">MARRHAEEGVARRGKVAVDGAWRPGRPCLAPPCPPRPHFTRSVSPSLGKKKGRLPRAAGQELLRPHGAGAGLPVLALLAFVSAAAAAAGNGGGGRKMMASHGEDADLKASPGAPAPAGGSAAQFRGQARLPRFATPRRYELRLRPDLVACTFTGSVAIAVVVSTPTRFLVLNAADLSVNRASIRFQNLVPTEVVFFKDDDVLVFGFSKQLPLGEGVLQMDYNGTLNDQMRGFYRRYCFSLRIEQTLVFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPS8</accession>
    <name>I1IPS8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100832366</name>
        <name evidence="7" type="ORF">BRADI_4g29243v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576475.1">
        <property type="nucleotide sequence ID" value="XM_003576427.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPS8"/>
    <dbReference type="STRING" id="15368.I1IPS8"/>
    <dbReference type="EnsemblPlants" id="KQJ90062">
        <property type="protein sequence ID" value="KQJ90062"/>
        <property type="gene ID" value="BRADI_4g29243v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832366"/>
    <dbReference type="Gramene" id="KQJ90062">
        <property type="protein sequence ID" value="KQJ90062"/>
        <property type="gene ID" value="BRADI_4g29243v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832366"/>
    <dbReference type="eggNOG" id="KOG1520">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023267_2_1_1"/>
    <dbReference type="OMA" id="NIVTCEL"/>
    <dbReference type="OrthoDB" id="5307922at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000048">
        <property type="entry name" value="Protein strictosidine synthase-like 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF31">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095421" description="Strictosidine synthase conserved region domain-containing protein" evidence="5">
        <location>
            <begin position="32"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="6">
        <location>
            <begin position="172"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ90062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="355" mass="37640" checksum="7753641336CE6357" modified="2012-06-13" version="1">MAYSFRPASSTASSFLALLLALPLVASLASAAGAAAVAPAAVYGTKSMDPGLAVMPLPSPVSGPESLAFDRRGGGPYAGVSDGRVLRWRGRRLGWTVFAYNSKHKSVGICAAKKLMVPESVCGRPLGLQFYHKTGELYVADAYLGLMRVPARGGMAEVVATEAGGVPFNFLNGLDVDQNTGDVYFTDSSATYRRSEYLLVVALGDETGRLLRYDPRARRVSVLHSDLSYPNGVAVSPDGTHVVVAHTAMSELRRYWVRGPRAGKSETFAELPGYPDNLRAVDGPRGGYWVALSREADGGGPAPTVAVRVGRDGAVEEALDGFSFVTVSEVSHRNGTLWVGSVDTPYAGALRLGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITT9</accession>
    <name>I1ITT9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3'(2'),5'-bisphosphate nucleotidase</fullName>
            <ecNumber evidence="3">3.1.3.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100844934</name>
        <name evidence="13" type="ORF">BRADI_4g40860v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>3'-phosphoadenylyl sulfate + H2O = adenosine 5'-phosphosulfate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:77640"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine 2',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:77644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:10041"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578819.1">
        <property type="nucleotide sequence ID" value="XM_003578771.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITT9"/>
    <dbReference type="FunCoup" id="I1ITT9">
        <property type="interactions" value="1494"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1ITT9"/>
    <dbReference type="EnsemblPlants" id="KQJ91962">
        <property type="protein sequence ID" value="KQJ91962"/>
        <property type="gene ID" value="BRADI_4g40860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844934"/>
    <dbReference type="Gramene" id="KQJ91962">
        <property type="protein sequence ID" value="KQJ91962"/>
        <property type="gene ID" value="BRADI_4g40860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844934"/>
    <dbReference type="eggNOG" id="KOG1528">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033446_3_0_1"/>
    <dbReference type="OMA" id="MSYQQER"/>
    <dbReference type="OrthoDB" id="411145at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01517">
        <property type="entry name" value="PAP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000003">
        <property type="entry name" value="PAP-specific phosphatase HAL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000016">
        <property type="entry name" value="SAL1 phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006239">
        <property type="entry name" value="DPNP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051090">
        <property type="entry name" value="Inositol_monoP_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01330">
        <property type="entry name" value="bisphos_HAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200:SF6">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ91962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="424" mass="44605" checksum="702AFB9B77E3D5F6" modified="2012-06-13" version="1">MAARVGLSHALLASSSLARNPPPRARLLPYPTLLPLPTYRRSVSASASALPSLRRRRPFAARAMSQPPEAAGSPYAAELAAAKKAVALAARLCQTVQQELVQSDVQSKADKTPVTVADYGSQILVSLVLNMEVTSGSFSMVAEEDSEDLRKEGAEEILEHITDLVNETLAEDGSFNISLSQEGILSAIDSGKSEGGPSGRHWVLDPIDGTKGFVRGGQYAIALALLDEGKVVLGVLGCPNLPLTSICNLNGNSSGDQTGVLFSATIGCGAEVQSLDGSPPQKISVCSIDNPVNASFFESYEGAHNMRDVTGSIAEKLGVQAPPVRIDSQAKYGALARGDGAIYLRFPHKGYKEKIWDHAAGSIVVTEAGGIVTDASGKDLDFSKGRCLDDLDTGIVATNKQLMPSLLKAVQEAIKEKNQAPSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVI3</accession>
    <name>I1IVI3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100845142</name>
        <name evidence="7" type="ORF">BRADI_5g00800v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579895.1">
        <property type="nucleotide sequence ID" value="XM_003579847.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVI3"/>
    <dbReference type="STRING" id="15368.I1IVI3"/>
    <dbReference type="EnsemblPlants" id="KQJ81453">
        <property type="protein sequence ID" value="KQJ81453"/>
        <property type="gene ID" value="BRADI_5g00800v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845142"/>
    <dbReference type="Gramene" id="KQJ81453">
        <property type="protein sequence ID" value="KQJ81453"/>
        <property type="gene ID" value="BRADI_5g00800v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845142"/>
    <dbReference type="eggNOG" id="KOG0143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010119_0_0_1"/>
    <dbReference type="OMA" id="HFILCHY"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000005">
        <property type="entry name" value="1-aminocyclopropane-1-carboxylate oxidase homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF872">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="210"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ81453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="363" mass="39208" checksum="98089C17CE1B5153" modified="2012-06-13" version="1">MAGAADYDRAADLRALDATFSGVHGLAASGITRLPRIFRAAVPDAAGQVQEPPPPPSPAAIPVIDLSGGRAATVAAVGRAAAEWGFFQVTGHGVAPEVASAAVRAARAFHETPGGEGSEKARLYSRDPAMAVKYNCNFDLHQSKLANWRDTLYLRMAPHPPPPADMPDSCRDVFFEYAEQVRNLWDKLFGLLSEALGLNTSHLSDMGCNKGQMILCHYYPPCPEPSHAVGTTRHSDSGFLTVLLQDAVGGLQILHGDRWLDVTPIPGAFIVNVGDLLQMVSNDRFRSVEHRVVLGAAKDAAMARVSIACFPSDPDSTRVYGPIKELLSEENPALYRETLARDYVAHYYSLGLGPKMAMNDFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVM6</accession>
    <name>I1IVM6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein AAR2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100823640</name>
        <name evidence="4" type="ORF">BRADI_5g01220v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580158.1">
        <property type="nucleotide sequence ID" value="XM_003580110.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239623.1">
        <property type="nucleotide sequence ID" value="XM_010241321.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVM6"/>
    <dbReference type="STRING" id="15368.I1IVM6"/>
    <dbReference type="EnsemblPlants" id="KQJ81519">
        <property type="protein sequence ID" value="KQJ81519"/>
        <property type="gene ID" value="BRADI_5g01220v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ81520">
        <property type="protein sequence ID" value="KQJ81520"/>
        <property type="gene ID" value="BRADI_5g01220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823640"/>
    <dbReference type="Gramene" id="KQJ81519">
        <property type="protein sequence ID" value="KQJ81519"/>
        <property type="gene ID" value="BRADI_5g01220v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81520">
        <property type="protein sequence ID" value="KQJ81520"/>
        <property type="gene ID" value="BRADI_5g01220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823640"/>
    <dbReference type="eggNOG" id="KOG3937">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036039_1_0_1"/>
    <dbReference type="OMA" id="VWQSGGL"/>
    <dbReference type="OrthoDB" id="201752at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IVM6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13778">
        <property type="entry name" value="Aar2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13777">
        <property type="entry name" value="Aar2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.550:FF:000002">
        <property type="entry name" value="AAR2 protein family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.20:FF:000001">
        <property type="entry name" value="protein AAR2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.550">
        <property type="entry name" value="Aar2, C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007946">
        <property type="entry name" value="AAR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033648">
        <property type="entry name" value="AAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038514">
        <property type="entry name" value="AAR2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033647">
        <property type="entry name" value="Aar2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038516">
        <property type="entry name" value="AAR2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689">
        <property type="entry name" value="A1 CISTRON SPLICING FACTOR AAR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689:SF4">
        <property type="entry name" value="PROTEIN AAR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05282">
        <property type="entry name" value="AAR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20981">
        <property type="entry name" value="AAR2_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAR2 N-terminal" evidence="3">
        <location>
            <begin position="24"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="AAR2 C-terminal" evidence="2">
        <location>
            <begin position="205"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ81520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="45748" checksum="AD899E35F0CD5216" modified="2012-06-13" version="1">MGSRSGGAAARMDPEAATELVRKGGTLLLLDVPQHTVFGIDTQVFSVGPKFKGIKMVPPGPHFVYYCSPSRHGNEFAPTVGFFLTTHTSEVIVRKWHAQEERLVTLSEEEEIRYSEAVKRFEFDDQLGPYNLDSFGDWKQLSSYLSPSVIERIEPIGGEITIAYETSWMDRAPQTDMEKRLMEQLREDKFAQNVSMQSERRGCYYTTIPASVKHKDISADELTLMNLDRTSLLESVLAKSYQGQEDLLLGELQFSFITFMMGQSLEAFMQWKALISLLLSCSEAPLHTRTNMFVKFIRVIYYQFKHGFLRTHESRSIEDKGNSLFLDEAWFSRDIFLYRLSKDFLSVILEAPVVDGDLLSRARELKTLLETTFGWDLENNAANLIDEDDEFAPVVVEMDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IXZ3</accession>
    <name>I1IXZ3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100842813</name>
        <name evidence="8" type="ORF">BRADI_5g10750v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579801.1">
        <property type="nucleotide sequence ID" value="XM_003579753.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311274.1">
        <property type="nucleotide sequence ID" value="XM_024455506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IXZ3"/>
    <dbReference type="STRING" id="15368.I1IXZ3"/>
    <dbReference type="EnsemblPlants" id="KQJ82744">
        <property type="protein sequence ID" value="KQJ82744"/>
        <property type="gene ID" value="BRADI_5g10750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842813"/>
    <dbReference type="Gramene" id="KQJ82744">
        <property type="protein sequence ID" value="KQJ82744"/>
        <property type="gene ID" value="BRADI_5g10750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842813"/>
    <dbReference type="eggNOG" id="KOG2494">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_817318_0_0_1"/>
    <dbReference type="OMA" id="IECKYAH"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000010">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054429">
        <property type="entry name" value="Znf-CCCH_Muscleblind-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675:SF12">
        <property type="entry name" value="PROTEIN MUSCLEBLIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22628">
        <property type="entry name" value="zf-CCCH_10"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="208"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="208"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="50"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ82744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="340" mass="39138" checksum="2A7C80BC7DF1D0B7" modified="2012-06-13" version="1">MASAEIPNAVTNDPNPTRSPSPPLPPRKRRLSLSPSRSPALSPALSPRRSSSRSRSPRRERSRSRSRSRSRSRSRSRNRTRSRSRSRSPRQPDGKRARHSDLTVEVCRDYLRDRCTRSDLECKYAHPHQSVSVDRDNKVTACADSLRNNCFRGRTCRYYHPPPHIQESLLRSIGVDAPPAKMVCRDFARGRCSRSANECRFLHHSSVQESAIVCQDFLRGRCDRISCRYTHVIAQQMFPPPMRDLPMQMQYPEMVYMAPPPPHGVPMMVPPLSPPTAFADHRRVEVCRDFLKNMCTRESCRFAHPDSRSEVPNDRVEVCRDFKRGECTRPNCRFFHPSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>I1IYB5</accession>
    <name>I1IYB5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100837337</name>
        <name evidence="13" type="ORF">BRADI_1g49990v3</name>
        <name evidence="14" type="ORF">BRADI_1g49997v3</name>
        <name evidence="12" type="ORF">BRADI_5g11920v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579868.1">
        <property type="nucleotide sequence ID" value="XM_003579820.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IYB5"/>
    <dbReference type="SMR" id="I1IYB5"/>
    <dbReference type="STRING" id="15368.I1IYB5"/>
    <dbReference type="EnsemblPlants" id="KQJ82897">
        <property type="protein sequence ID" value="KQJ82897"/>
        <property type="gene ID" value="BRADI_5g11920v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK19721">
        <property type="protein sequence ID" value="KQK19721"/>
        <property type="gene ID" value="BRADI_1g49990v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT76594">
        <property type="protein sequence ID" value="PNT76594"/>
        <property type="gene ID" value="BRADI_1g49997v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837337"/>
    <dbReference type="Gramene" id="KQJ82897">
        <property type="protein sequence ID" value="KQJ82897"/>
        <property type="gene ID" value="BRADI_5g11920v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19721">
        <property type="protein sequence ID" value="KQK19721"/>
        <property type="gene ID" value="BRADI_1g49990v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76594">
        <property type="protein sequence ID" value="PNT76594"/>
        <property type="gene ID" value="BRADI_1g49997v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829931"/>
    <dbReference type="KEGG" id="bdi:100830542"/>
    <dbReference type="KEGG" id="bdi:100837337"/>
    <dbReference type="eggNOG" id="KOG1745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078295_4_0_1"/>
    <dbReference type="OMA" id="HIFAEMA"/>
    <dbReference type="OrthoDB" id="1861411at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IYB5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000078">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000044">
        <property type="entry name" value="Histone H3.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="11">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ82897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK19721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="PNT76594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="136" mass="15406" checksum="6D72383BF2E9EBE6" modified="2012-06-13" version="1">MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZ30</accession>
    <name>I1IZ30_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100832293</name>
        <name evidence="3" type="ORF">BRADI_5g14030v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580020.2">
        <property type="nucleotide sequence ID" value="XM_003579972.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZ30"/>
    <dbReference type="EnsemblPlants" id="KQJ83271">
        <property type="protein sequence ID" value="KQJ83271"/>
        <property type="gene ID" value="BRADI_5g14030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832293"/>
    <dbReference type="Gramene" id="KQJ83271">
        <property type="protein sequence ID" value="KQJ83271"/>
        <property type="gene ID" value="BRADI_5g14030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832293"/>
    <dbReference type="eggNOG" id="ENOG502RRNC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030845_6_1_1"/>
    <dbReference type="OMA" id="RVILAEC"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IZ30">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046533">
        <property type="entry name" value="DUF6598"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065">
        <property type="entry name" value="OS07G0486400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065:SF88">
        <property type="entry name" value="OS11G0104220 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20241">
        <property type="entry name" value="DUF6598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6598" evidence="2">
        <location>
            <begin position="243"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ83271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="513" mass="58649" checksum="26AC183889672F79" modified="2012-06-13" version="1">MEIDDSWKMGIDDSGKEMHWESTRFTRTRVFREMGIIVTTEIISLLMEIDFLPRDQEILVDNLLPLQGKSLLVRRKNRNQLRSGPSRRFPEREHMPDQASKSSHVSSISEQMSMCSSEIQEKLVQYFNFLLHEMKRVGYMMKHMASIKRETGIPISVDKIVDLLQSTSRLTQISCRVVEMMKEITKSVEETYADHRAGWENTWGNKSGLRIGFEDLTTLSPMHFTYRQPGLIPRGYTSICASTLQIYSLEITELKGNLKWPLYVYGVVAARDTVDHKRNLLFCRSRANCQEVTENAKFLHLIGPSRAILALDYVHFEFELKMKDGKSDRDLITRAYRYHGRHHTCNISNWNCALELSLDRLLETVQATILSVRVGEGESWPFEYGVRVACYSQSLEVVEVDSEGVVGEVTGPKSGQVDLLHCCDKPVPLGPDGYLHLSRQVVSVEQQDCLKVVIEAYSSPKLDIAARGHIYFPQEYCNISHGTCLLGQLKVEISVAWSYLAADKLHLLKEEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZM6</accession>
    <name>I1IZM6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100840483</name>
        <name evidence="8" type="ORF">BRADI_5g15587v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580130.1">
        <property type="nucleotide sequence ID" value="XM_003580082.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZM6"/>
    <dbReference type="FunCoup" id="I1IZM6">
        <property type="interactions" value="1445"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IZM6"/>
    <dbReference type="EnsemblPlants" id="KQJ83573">
        <property type="protein sequence ID" value="KQJ83573"/>
        <property type="gene ID" value="BRADI_5g15587v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840483"/>
    <dbReference type="Gramene" id="KQJ83573">
        <property type="protein sequence ID" value="KQJ83573"/>
        <property type="gene ID" value="BRADI_5g15587v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840483"/>
    <dbReference type="eggNOG" id="ENOG502QWSH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035664_5_3_1"/>
    <dbReference type="OMA" id="NGSKGTM"/>
    <dbReference type="OrthoDB" id="730183at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000006">
        <property type="entry name" value="NAC domain-containing protein 100-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF4">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="10"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ83573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="288" mass="32197" checksum="D9339B1E305CF205" modified="2012-06-13" version="1">MGLREIESTLPPGFRFYPSDQELVCHYLYKKVTNERASQGTLVEVDLHAREPWELPDVAKLTASEWYFFSFRDRKYATGSRTNRATKTGYWKATGKDREVRNPSTRAVVGMRKTLVFYQGRAPNGTKTSWVMHEFRLDTPHLPPREDWVLCRVFEKQKGDGEQDNISASSPAFAGSSQAAAAQEPHQPMLDTSNDQIMGSAMGFVPRQDQEEVCGSNYPLLNAAMWQQYSSVLDTFPLQEEIMVGSSPMMGLGAARSGAGDDCGFLFDSGFDDVANFGAGMRFPQGWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZZ8</accession>
    <name>I1IZZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC100837222</name>
        <name evidence="24" type="ORF">BRADI_5g16590v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240174.1">
        <property type="nucleotide sequence ID" value="XM_010241872.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZZ8"/>
    <dbReference type="FunCoup" id="I1IZZ8">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83743">
        <property type="protein sequence ID" value="KQJ83743"/>
        <property type="gene ID" value="BRADI_5g16590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837222"/>
    <dbReference type="Gramene" id="KQJ83743">
        <property type="protein sequence ID" value="KQJ83743"/>
        <property type="gene ID" value="BRADI_5g16590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837222"/>
    <dbReference type="eggNOG" id="ENOG502QSJ4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_62_3_1"/>
    <dbReference type="OMA" id="WERKRLY"/>
    <dbReference type="OrthoDB" id="543442at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901001">
        <property type="term" value="P:negative regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative receptor kinase Lecrk"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
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        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
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        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095648" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="30"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="354"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQJ83743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="678" mass="73965" checksum="2A8E3C48A502EE44" modified="2012-06-13" version="1">MLLPNQTMPFLLLLLFLGLSGLLRPAASADEQFVFNGFTGANLSMDGMARVTPNGLLQLTNATSQLKGHAFFPTPLQFHRAPNSTAMQSFSTAFVIGIIGAYEDLSSHGMAFIIAKSRNLTSALPGQFLGLVGSANNGNATNHLFAVEFDTILNSEFNDMSGNHVGIDVNGLNSVDADNAGYYDDGTGAFKNMSLVNRKPMQVWVDFDGQAMQVNVTMAPLEVVARPKKPLLSTTVNLSSVIDDTAYVGFSSSSGILFCRHYVLGWSLKMNGAAPALNISSLPSMPVTTPKPRSRTLEIVLPIASAVLVFVVAAVVFVVMRRRRMFAELKEDWEVAFGPHRFAYKDLFHATDGFSDSRLLGIGGFGRVYRGLLPSSKAEIAVKKVSHGSRQGMKEFVAEVVSIGRLRHRNLVQLLGYCRRKGELLLVYDYMPNGSLDKHLYDQQKVSLSWGQRFRIIKGVASGMLYLHEDWDQVVVHRDIKASNVLLDAEMNGRLGDFGLARLYDHGTDPHTTHVVGTMGYLAPELGHTGKASKASDVFAFGAFMLEVACGRKPVVQDASDNHLVLVDWVLDQWRAGSVTGAVDPRLGGDFVEHEASLVLRLGLLCSHPLPGARPTTRQLVQYLDGDVKLPELSPTYQSFNMLAVMQDQGFDPYVMSFPMTSITAGTMSQMSDLSGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0B0</accession>
    <name>I1J0B0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100845263</name>
        <name evidence="11" type="ORF">BRADI_5g17527v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751103.1">
        <property type="nucleotide sequence ID" value="XM_014895617.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311288.1">
        <property type="nucleotide sequence ID" value="XM_024455520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0B0"/>
    <dbReference type="STRING" id="15368.I1J0B0"/>
    <dbReference type="EnsemblPlants" id="KQJ83915">
        <property type="protein sequence ID" value="KQJ83915"/>
        <property type="gene ID" value="BRADI_5g17527v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845263"/>
    <dbReference type="Gramene" id="KQJ83915">
        <property type="protein sequence ID" value="KQJ83915"/>
        <property type="gene ID" value="BRADI_5g17527v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845263"/>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000837_25_4_1"/>
    <dbReference type="OMA" id="RAKRCCI"/>
    <dbReference type="OrthoDB" id="598235at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF943">
        <property type="entry name" value="OS08G0193700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="11"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="171"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="431"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="548"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ83915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="925" mass="104161" checksum="E63961F8D76AF95B" modified="2012-06-13" version="1">MAEMIAISLSAKVAAALSRKAAIDLSSLVAIRSGIAAAARDLELLRAFLRFADSRRVTDALASAWVDQVRDVGFELEDVADEYVFLSGSGFIRACANIGAWFALARRLRKARERLRDLSGAKERYGIRPAQASASSSAPDGGTVPAIGRKLAEAAHFVEDEEIVGFVAHRRSLMEWLTEDTHSRRTLVSVCGMGGVGKTTLVTNVYNEIAASRHFDCAAWVAVSKKFTPEDLLRKIAKELHRGVSAGMPWDINEMDYLSLVEALRGHLARKRYLLLLDDVWDAHAWYEIRSAFVDDGTGSRIIITTRSQDVASLAASNRIIMLEPLPEKEAWSLFCNTTFREDANRECPYHLQNWAFKILDRCCGLPLAIVSVGNLLALKQKTEFAWKNVHDSLEWNESSDRGIEQVSSILNLSIDDLPYHLKRCFLHCSIYPEDFSIKRKILTRLWIAEGYIEEKGQRTMEEIADDYLSQLVHRSLLRVTLKNEFGRAKRCCIHDLIRELIVQRSTKEGFFVFSGCTATMVSNKKIRHLILDRCRSDHLPASKMTLLRTFTAFMADVDVALLSGFRLLTVLNLWFVPIAELPTSVTNLRNLRYLGIRSTFIEELPQDLGQLHNLQTLDTKWSMVQRLPPSIRNLKSLRHLIVFRRRSADFRYAGPGTAIEFPDGLQYLTCLQTLKHIEADEKMVKSLGSLKHMKSLELCGVHESNLVHLPSSISTMSGLLSLGIVSRDANVTLDLEPFYPPPLKLQKLSLTGMLARGKLPSWFGNLDNLMQLRLCSSALKGDSIELLSLLPRLLHLNLNNAYNDKSLTFAEGCFPVLKKLSLHGLPNLSHIEFQKGSLVHLNVLILGCCAELTEIPQGMENLIQLDNLELFEMPSEIVQKMQDGEVLGENHEDARRTTTVKNTRWHNGQLLEKTIYTNLFDVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0K4</accession>
    <name>I1J0K4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100831106</name>
        <name evidence="5" type="ORF">BRADI_5g18307v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751272.1">
        <property type="nucleotide sequence ID" value="XM_014895786.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0K4"/>
    <dbReference type="EnsemblPlants" id="KQJ84042">
        <property type="protein sequence ID" value="KQJ84042"/>
        <property type="gene ID" value="BRADI_5g18307v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831106"/>
    <dbReference type="Gramene" id="KQJ84042">
        <property type="protein sequence ID" value="KQJ84042"/>
        <property type="gene ID" value="BRADI_5g18307v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831106"/>
    <dbReference type="eggNOG" id="ENOG502R87M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015101_0_2_1"/>
    <dbReference type="OMA" id="LDTACCG"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF57">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095686" description="GDSL esterase/lipase" evidence="4">
        <location>
            <begin position="25"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ84042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="41748" checksum="59529775DAAE5ED6" modified="2012-06-13" version="1">MASRRPSWALALVALCLSFELALQEKGKGKKEDDNNGGGGGGGETLVPAIFVFGDSTVDVGNNNFLPRCNDICRANYPRYGVDYPSQNATGRFSNGYNLADYVAKLLGFPESPPALKSLSEEGIIEQMKKGINYASGGSGLRNHTGHDLCGQVCTMADQLEMFTSNVQKMGKEDSSELISRSLFFISVGSNDLFEYANDSKPRHNRNDTEFLKGLVDLYKSYLQELYEVGARKFSVVSPSLVGCCPIQRVLGNQRNDTDGLGCFGTANNLSRQLYPMMLSMLQNIDLQGMNYSIADSVGMTELVFEGVIPPGMNLTVVDTACCGGSGPLQVDKCNSTATLCPNRDNYLFWDGFHATDVASSGAAKMLCSDEGSFVHPINITKLATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0L5</accession>
    <name>I1J0L5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein LAZ1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100824148</name>
        <name evidence="7" type="ORF">BRADI_5g18390v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580331.1">
        <property type="nucleotide sequence ID" value="XM_003580283.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0L5"/>
    <dbReference type="FunCoup" id="I1J0L5">
        <property type="interactions" value="822"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J0L5"/>
    <dbReference type="EnsemblPlants" id="KQJ84055">
        <property type="protein sequence ID" value="KQJ84055"/>
        <property type="gene ID" value="BRADI_5g18390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824148"/>
    <dbReference type="Gramene" id="KQJ84055">
        <property type="protein sequence ID" value="KQJ84055"/>
        <property type="gene ID" value="BRADI_5g18390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824148"/>
    <dbReference type="eggNOG" id="KOG2641">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012923_0_0_1"/>
    <dbReference type="OMA" id="KPWRLGM"/>
    <dbReference type="OrthoDB" id="5348404at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900458">
        <property type="term" value="P:negative regulation of brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098876">
        <property type="term" value="P:vesicle-mediated transport to the plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="394"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ84055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="53544" checksum="8CDE1D3DA2201F43" modified="2012-06-13" version="1">MRVNLELLMPLMAQYTAPTWATLVAGFFVLLALSLSMYLIFEHLSAYNNPEEQKFVLGVILMVPCYAIESYVSLIDPNTNVYCGILRDGYEAFAMYCFGRYITACLGGEDKTIAFLKREGGSGSGQPLLHDAPEKAIIHHHFPVNLILKPWRMGTRFYQIIKFGIFQYVIIKTLTASLSLFLEAFGVYCEGEFNLRCGYPYFAAVLNFSQFWALYCLVEWYTATKDELAHIKPLAKFLSFKSIVFLTWWQGVVIAIMYALGMLRSPLAQSLELKSSIQDFIICIEMGIASVVHLYVFPAKPYELLANQSPGNISVLGDYVSSEPVDPYEIRESNRPSKMKLPQFEPDERSATNIKESVRDFVIGSGEYVIKDFKFTVNQAVRPVEKRFDKLMKKKDKYKKSQDDNWVSAKSPERPVRGIDDPLLSGSTSDSGVMKGKKHRRDFSSVAAVDSWGGGDKTPDGYEIRGRRWAVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1J1W4</accession>
    <name>I1J1W4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100837235</name>
        <name evidence="16" type="ORF">BRADI_5g21960v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010227269.1">
        <property type="nucleotide sequence ID" value="XM_010228967.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J1W4"/>
    <dbReference type="STRING" id="15368.I1J1W4"/>
    <dbReference type="EnsemblPlants" id="KQJ84633">
        <property type="protein sequence ID" value="KQJ84633"/>
        <property type="gene ID" value="BRADI_5g21960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837235"/>
    <dbReference type="Gramene" id="KQJ84633">
        <property type="protein sequence ID" value="KQJ84633"/>
        <property type="gene ID" value="BRADI_5g21960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837235"/>
    <dbReference type="eggNOG" id="ENOG502QTTB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_22_1_1"/>
    <dbReference type="OMA" id="ALPRHCN"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000676">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000299">
        <property type="entry name" value="Piriformospora indica-insensitive protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF83">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095656" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="36"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="834"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="900"/>
            <end position="1223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQJ84633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1223" mass="129126" checksum="E00438A3B57FB3B6" modified="2012-06-13" version="1">MVPPKKTRDASVLPLHVLAFLALAALLGSSPRAAAASASVQLEALLEFKKGVTADPLGALSGWQKKADSRNAIAAAAIVPPPHCNWTGIACNIAGQVTSIQLLESQLEGTLTPFLGNITTLQVLDLTSNAFFGLIPPELGRLQSLEGLILTVNTFTGVIPTSLGALTSLQILDLSNNSLHGTIPSQLCNCSAMWALGLEANNLTGQIPPCIGDLSNLEIFQAYINSLSGELPRSFANLTKLTTLDLSGNQLSGRVPPAIGTFSGLKILQLFENRFSGKIPPELGNCKNLTLLNIYSNRFTGAIPRELGGLTNLKALRVYDNALSSTIPSSLRRCSSLLALGLSMNELTGNIPPELGELRSLQSLTLHENRLTGTVPKSLTRLVNLMRLSFSDNSLSGPLPEAIGSLRNLQVLIIHGNSLSGPIPASIVNCTSLSNASMAFNGFSGSLPAGLGRLQSLVFLSLGDNSLEGTIPEDLFDCVRLRTLNLAENNLTGRLSPRVGKLGGELRLLQLQGNALSGSIPDEIGNLTRLIGLTLGRNKFSGRVPGSISNLSSSLQVLDLLQNRLSGALPEELFELTSLTVLTLASNRFTGPIPNAVSKLRALSLLDLSHNMLNGTVPAGLSGGHEQLLKLDLSHNRLSGAIPGAAMSGATGLQMYLNLSHNAFTGTIPREIGGLAMVQAIDLSNNELSGGVPATLAGCKNLYTLDISSNSLTGELPAGLFPQLDLLTTLNVSGNDFHGEILPGLAGMKHLQTVDVSRNAFEGRVPPGMEKMTSLRELNLSWNRFEGPVPDRGVFADIGMSSLQGNAGLCGWKKLLAPCHAAAGNQRWFSRTGLVTLVVLLVFALLLLVLVVAILVFGHRRYRKKKGIESGGHVSSETAFVVPELRRFTYGELDTATASFAESNVIGSSSLSTVYKGVLVDGKAVAVKRLNLEQFPAMSDKSFLTELATLSRLRHKNLARVVGYAWEREAAGNGNGNRMMKALVLEYMDNGDLDAAIHGGGRGALDAHTAPPRWATVAERLRVCVSVAHGLVYLHSGYGGSPVVHCDVKPSNVLMDADWEAHVSDFGTARMLGVQLTDAPAQETGTSSAFRGTVGYMAPELAYMRSVSPKADVFSFGVLVMELLTKRRPTGTIEDDGSGVPVTLQQLVGNAVSMGIEAVAGVLDADMSKAATDADLCAAAGALRVACSCAAFEPADRPDMNGALSALLKISNACGPGKTRIDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1J2L7</accession>
    <name>I1J2L7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Oryzain alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100831807</name>
        <name evidence="13" type="ORF">BRADI_5g24027v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT62012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J2L7"/>
    <dbReference type="FunCoup" id="I1J2L7">
        <property type="interactions" value="1161"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J2L7"/>
    <dbReference type="MEROPS" id="C01.029"/>
    <dbReference type="EnsemblPlants" id="PNT62012">
        <property type="protein sequence ID" value="PNT62012"/>
        <property type="gene ID" value="BRADI_5g24027v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62012">
        <property type="protein sequence ID" value="PNT62012"/>
        <property type="gene ID" value="BRADI_5g24027v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4296">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012184_0_1_1"/>
    <dbReference type="OMA" id="CIYEYAN"/>
    <dbReference type="OrthoDB" id="10253408at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.160:FF:000002">
        <property type="entry name" value="Cysteine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000068">
        <property type="entry name" value="Cysteine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.160">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000118">
        <property type="entry name" value="Granulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037277">
        <property type="entry name" value="Granulin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00396">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00277">
        <property type="entry name" value="GRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57277">
        <property type="entry name" value="Granulin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005398730" description="Oryzain alpha chain" evidence="9">
        <location>
            <begin position="23"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="12">
        <location>
            <begin position="42"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="11">
        <location>
            <begin position="131"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="10">
        <location>
            <begin position="369"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="PNT62012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="49715" checksum="2A118063357DA751" modified="2012-06-13" version="1">MRSSMALVAAAALLLLVSLAAAADMSIVSYGERSEEEVRRMYVEWMAENGRTYNAIGEEERRFEVFRDNLRYVDQHNAAADAGLHSFRLGLNRFADLTNEEYRDTYLGVRTKPVRERRLSGRYQAADNEELPESVDWREKGAVAKVKDQGGCGSCWAFSAIAAVEGINQIVTGDMIALSEQELVDCDTSYNQGCNGGLMDYAFEFIINNGGIDSEEDYPYKERDNRCDANKKNAKVVTIDGYEDVPVNSELSLKKAVANQPISVAIEAGGRAFQLYKSGIFTGRCGTALDHGVTAVGYGSENGKDYWIVKNSWGTVWGEDGYVRLERNIKATSGKCGIAIEPSYPLKKGANPPNPGPTPPSPAPPSTVCDSYNECPASTTCCCIYTYGKECFAWGCCPLEGATCCDDHYSCCPHSYPICNVQQGTCLAGKDSPMSVKALKRILAKPHGTFSGKSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EJ25</accession>
    <name>A0A0Q3EJ25_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ent-kaurenoic acid monooxygenase</fullName>
            <ecNumber evidence="14">1.14.14.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BRADI_4g05240v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ent-7alpha-hydroxykaur-16-en-19-oate + reduced [NADPH--hemoprotein reductase] + O2 = gibberellin A12 aldehyde + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22904"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57298"/>
            <dbReference type="ChEBI" id="CHEBI:57432"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:22905"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ent-kaur-16-en-19-oate + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = gibberellin A12 + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:33219"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57297"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58627"/>
            <dbReference type="EC" id="1.14.14.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:33220"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ent-kaur-16-en-19-oate + reduced [NADPH--hemoprotein reductase] + O2 = ent-7alpha-hydroxykaur-16-en-19-oate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19241"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57297"/>
            <dbReference type="ChEBI" id="CHEBI:57298"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19242"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>gibberellin A12 aldehyde + reduced [NADPH--hemoprotein reductase] + O2 = gibberellin A12 + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22700"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57432"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58627"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:22701"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Plant hormone biosynthesis; gibberellin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.107" evidence="14"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EJ25"/>
    <dbReference type="STRING" id="15368.A0A0Q3EJ25"/>
    <dbReference type="EnsemblPlants" id="KQJ86404">
        <property type="protein sequence ID" value="KQJ86404"/>
        <property type="gene ID" value="BRADI_4g05240v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ86404">
        <property type="protein sequence ID" value="KQJ86404"/>
        <property type="gene ID" value="BRADI_4g05240v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EJ25"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051777">
        <property type="term" value="F:ent-kaurenoic acid monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009686">
        <property type="term" value="P:gibberellin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048868">
        <property type="term" value="P:pollen tube development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000052">
        <property type="entry name" value="Ent-kaurenoic acid oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002397">
        <property type="entry name" value="Cyt_P450_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF356">
        <property type="entry name" value="ENT-KAURENOIC ACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00359">
        <property type="entry name" value="BP450"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 19" id="KW-0349">Heme</keyword>
    <keyword evidence="10 19" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037909"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00066318"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00093181"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00093243"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00093246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KQJ86404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="479" mass="54228" checksum="4EF9213E6DFD236F" modified="2016-01-20" version="1">MGEETAVGMWYFAAVAMAAAVLAVVVDAVVRRAHAWWWTASLGAGRRARLPPGDMAEICMANGRFGRTGLYRAFMFSSPTILVTIPEACKQVLMDDEAFILGWPKSTVTLIGAKSFIAIPHEEHRRLRKLTAAPINGLDALTAYLGFIDHTVVSTLQALSTREEEVEFLTELRRMTFRIIVQIFMSGADDRTVEALERCYTDLNYGLRAMAINLPGFAYHRALKACKKMVELLQHVLDQRRAATAKGFPRPSVDMMDRLIKVEDEHGRRLEDDAIIDMLVMYLNAGHESSGHITMWATVFMQENPDILAKAKAEQEEIMRNIPPTQKGLSLKDFRKMEYLSQVIDETVRFMSINFVTFRQATRDVFVNGYLIPKGWKVQLWFRSVHTDPQIHPHPNKFNPSRWEGPPPRAGTFLAFGLGPRLCPGNDLAKLEISVFLHHFLLGYKLTRANPSCRLRYLPHVRPVDNCLAKITKISSHGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EY19</accession>
    <name>A0A0Q3EY19_BRADI</name>
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        <recommendedName>
            <fullName evidence="1">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g42752v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Magnoliopsida</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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            <begin position="2"/>
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        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ92302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="117" mass="12691" checksum="F619D0A5FCC12DFE" modified="2016-01-20" version="1">MNRIDGDGGSVDITLALIRRAVEAMIEVVVSEVSFVSGIGKEIQLFHGVVDEPCGLRRSVVAVALDTLMELKFKVCHGCGEIERCANFKAIKHGSVSQDIKFDQASISVKVTWSTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F0F4</accession>
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="2 3" key="1">
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3F0F4"/>
    <dbReference type="EnsemblPlants" id="KQJ93110">
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        <property type="gene ID" value="BRADI_3g02790v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93110">
        <property type="protein sequence ID" value="KQJ93110"/>
        <property type="gene ID" value="BRADI_3g02790v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3F0F4"/>
    <dbReference type="OrthoDB" id="199922at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3F0F4">
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    </dbReference>
    <dbReference type="InterPro" id="IPR018962">
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    </dbReference>
    <dbReference type="InterPro" id="IPR044687">
        <property type="entry name" value="LPA3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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            <begin position="74"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ93110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="232" mass="25694" checksum="AFCF1941319B6B27" modified="2016-01-20" version="1">MAMATSYSISNPTFTSKSSLPNKQVPNWIFPIISSDNGSGGMFTLARRSLRAGFHVCAVTGDQNTRNVFSANFPSDYTELLLQAKDAAESAFKDGKQLLEIEFPTAGLQSVPGDGEGGIEMTGSMLLIREFCDRFVPAEKTTRTRIFFPEANEVTFARQSAFEGCSLKLDYLTKPSLFEDFGFTTKVKMADRVQPEDEIFLVAYPYFNVNGNALLMLLNLSYPPHTLLFSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F5Z7</accession>
    <name>A0A0Q3F5Z7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g15225v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F5Z7"/>
    <dbReference type="EnsemblPlants" id="KQJ95103">
        <property type="protein sequence ID" value="KQJ95103"/>
        <property type="gene ID" value="BRADI_3g15225v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95103">
        <property type="protein sequence ID" value="KQJ95103"/>
        <property type="gene ID" value="BRADI_3g15225v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3F5Z7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ95103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="63" mass="7375" checksum="BDA383CDBF82EA9F" modified="2016-01-20" version="1">MSGYHVLQQRTSGHCRSIYGEVEQRFCQKLRSSAFELCSRLIFSFVCFILFIFPCNLVSTLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F7B7</accession>
    <name>A0A0Q3F7B7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_3g18271v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F7B7"/>
    <dbReference type="EnsemblPlants" id="KQJ95639">
        <property type="protein sequence ID" value="KQJ95639"/>
        <property type="gene ID" value="BRADI_3g18271v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95639">
        <property type="protein sequence ID" value="KQJ95639"/>
        <property type="gene ID" value="BRADI_3g18271v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3F7B7"/>
    <dbReference type="OrthoDB" id="660757at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18280">
        <property type="entry name" value="BTB_POZ_BPM_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF474">
        <property type="entry name" value="OS08G0228200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="137"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ95639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="304" mass="33734" checksum="666047DF8ADFD0C6" modified="2016-01-20" version="1">MPSEISKTASRCTVERARGTHTFEIVGYEDIKTGDPVQSATLAVGGYDWALGFYRDGRKDYSGQCGLVFLRLMNNKAEVRGVYDLRMIAFECVLTVIGEPRIAETVRVSRIEVPLSNLSDHFGKLLLEQEGVDIAGVGGETFSAHKIVLATRSPVFKAELYGQMKERTASRVTIQDMQPAVFKAFLHFIYTDSLPADMYDPDAADGDSEMIRHLLVAADRYAMDRLKLVCQSFLCEYYVDAETVATTLALADQHNCDKLKDACIEFIASSEDAVVATKGYASLKRTCPSLLIDALEKAHKFRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FE86</accession>
    <name>A0A0Q3FE86_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carnitine/acylcarnitine carrier-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106866561</name>
        <name evidence="11" type="ORF">BRADI_3g34077v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756942.2">
        <property type="nucleotide sequence ID" value="XM_014901456.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FE86"/>
    <dbReference type="STRING" id="15368.A0A0Q3FE86"/>
    <dbReference type="EnsemblPlants" id="KQJ97911">
        <property type="protein sequence ID" value="KQJ97911"/>
        <property type="gene ID" value="BRADI_3g34077v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106866561"/>
    <dbReference type="Gramene" id="KQJ97911">
        <property type="protein sequence ID" value="KQJ97911"/>
        <property type="gene ID" value="BRADI_3g34077v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="14252at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FE86">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000050">
        <property type="entry name" value="mitochondrial carnitine/acylcarnitine carrier-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF12">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="68"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="168"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="293"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ97911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="38795" checksum="581F9BE30ADA1763" modified="2016-01-20" version="1">MAMGPLFLLVREWVVGSALSWHLNHLNLLLLGLFFSPLVSALFPVDWVLCQAPLPRSTGSEFARAEMGDVARDLGAGTVGGAAQLVVGHPFDTIKVKLQSQPTPPPGQPPKFAGAIDAVKQTLAAEGPRGLYKGMGAPLATVAAFNAVLFTVRGQMEAALRSSPGAPLTVAQQVAAGAGAGVAVSFLACPTELIKCRLQAQSSLPTPAPAPAAAAAPVAGATATVSAAAAAGPKYGGPMDVARHVLRSEGGVRGLFKGLGPTLAREVPGNAVMFGVYEATKQAMAGGQDTSGLGRGSMVVAGGVAGAAFWGSVYPTDVVKSVLQVDDYKNPKYSGAMDAFKKILAADGVKGLYRGFGPAMARSVPANGACFLAYEVTRSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FVW2</accession>
    <name>A0A0Q3FVW2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g08641v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK03568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="486" mass="55407" checksum="8AC1A2476D948FB9" modified="2018-03-28" version="2">MLNLATFQLVQRVAGLDSEPPLDLSNGLKNKMLSVGLDNAQELLQRIKSETSKMKAMIIDFEPSYNKDIRPKLASSCLLAPGPITDPWTVFLHRQTFKIANVNPSYLSPFDIMSYKYGWKDFQWDMLERYLVQRRIYIIDDWESSLSTIETLMYDHGLKEPDDGTDIPFYKGECDASYDEHTKTANLSYMLWDDEKKIKHSEVLLSVKCSSATEAKIFAALALLYKAKELGLIKILNGELAIGKNHKHCDLYLMLRSSRILFQWLVLSWRPRELMVFVDALAKSETRFLSPAKFAVEKWADHLQGLPVFRIERTKEAKTAIKKFDNVTLDSSFGVIPELHYFLQVEEENKLDSLVRLIHSLKPSAIKVTFNNTDKLDSFKEKLINVIGIPIPGEDSTYTSGPFTTYTSQPKTHTSGSKCLMVVFDYVVPKHAYYTDNGFHVLFVTAREQELIGGEMTELNALGFIYINGGSWQSHLPTLLSFVAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FZP8</accession>
    <name>A0A0Q3FZP8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cysteine proteinase inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100826431</name>
        <name evidence="12" type="ORF">BRADI_2g10146v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Specific inhibitor of cysteine proteinases. Probably involved in the regulation of endogenous processes and in defense against pests and pathogens.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cystatin family. Phytocystatin subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK03845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_014899240.2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FZP8"/>
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        <property type="protein sequence ID" value="KQK03845"/>
        <property type="gene ID" value="BRADI_2g10146v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK03845"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4 10" id="KW-0646">Protease inhibitor</keyword>
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            <begin position="38"/>
            <end position="128"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK03845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="242" mass="26697" checksum="C2B7FCD189B21C24" modified="2016-01-20" version="1">MRVAATRPFFPAPGALFAALALVLVGSASLAIGAMASHVLGGKRESPAAANSLETDGLARFAVDEHNKRENALLEFVRVVEAKEQTVAGTLHHLTLEAIEAGRKKVYEAKVWVKPWLDFKELQEFRHTGDATSFTTSDLGAKKGEHEPGWRDVPVHDPVVKEAASHAVKSIQERSNSLFPYELLEIIRAKAEVVEDFAKFDILLKLKRGTKEEKIKAEVHKNLEGAFVLNQMQPEHDESSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G0P0</accession>
    <name>A0A0Q3G0P0_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BRADI_2g17899v3</name>
    </gene>
    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="BRADI_2g17899v3"/>
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        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK05088.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="99" mass="10355" checksum="1D58FC097933DA16" modified="2018-03-28" version="1" fragment="single">LQRCLLRPPPPPPPPAALSSAPPQPASHAPHPVSALLFNSRCRRPCPLASSCTPAPPAASVPRRTSSAAFAQARPAVSQALQRSAVSVAIHIRFTKEVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GBZ8</accession>
    <name>A0A0Q3GBZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_2g39207v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GBZ8"/>
    <dbReference type="EnsemblPlants" id="KQK08045">
        <property type="protein sequence ID" value="KQK08045"/>
        <property type="gene ID" value="BRADI_2g39207v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08045">
        <property type="protein sequence ID" value="KQK08045"/>
        <property type="gene ID" value="BRADI_2g39207v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GBZ8"/>
    <dbReference type="OrthoDB" id="611041at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GBZ8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1167">
        <property type="entry name" value="OS08G0412100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999564" description="AAA+ ATPase domain-containing protein" evidence="7">
        <location>
            <begin position="18"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="11"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="177"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="438"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="542"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK08045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="915" mass="102933" checksum="1F7E662AD44EDD4D" modified="2016-01-20" version="1">MAPVVSAALGALGPLLAKLTTLLADECGRLKGVRREIRSLRSELTSMHAALQSYSMLEDPSLQVKAWISLVRELAYDTEDCIDKFIQQLGDGRQQGGIKEVFRNTVRRLKTLRSRRRIASQIGDLKARVIEVQEQKNRYKLDDIPRSTSDHVAVDPRLSALFAEEAHLVGIEGPRDDLAKWMVEEENSPVEHHRSVLSIVGSGGLGKTTLANQIYRKIQGHFQCQAIVSVSQKPDTKKIVKDVISQVPCQDGFTKDIDTWDEKKSIAKLRELLQDKRYLIIIDDIWSIPAWDAIKCAFPENNCSSKIIATTRIFAVARSCCPGDDDRIYEMEPLSAPHSERLFFRRIFGSENCCPDMFKEVSNEILKKCGGLPLAIISISSLLANRPVVKEEWERVKRSIGSALDKDKSLEGVNSILSLSYNDLPPNLKTCLLYLSHFPEDYVIERERLVRRWIAEGFISEERGQTQQEVAENCFYELINKSMVQAVDIGYDGKASACRNFITVINGAQAIWENSHGYIRRLSIQHIDQELASVLANKDLRHVRSLTVTDSDCIKHLPSIAQFETLRVLDLEGCEGLEEYDMNGMDKLFHLKYLSFRSTGIPELPPGSVMLHNLETLDLRNTDIQELPAGITELVKLKHLLIVRDTYGELEGNTKIPNGIGNMRNLRAVSGFNITMSSKAAVEELGNLTSLDHLHVELDSGGSEEYKEMLLSSLCKLGASKLQSLELYSKEPTPLEFLDSWSPLPSSLQLFNMDTYYYLPRTPKWITPALTSLAYLDINLIEVTDDDLRRLGKLHALLSLELWLKSDPRERLKVQGFPSLKEFILVCRDNHGGAYVTFVEGAMPKLENLELPFHVSMARAYGFCFSIGHLPCLKEAEVHLYNKGAKYSETKAAAAVIRTEAGIHPNHPSVTIVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H7A0</accession>
    <name>A0A0Q3H7A0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_1g45275v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H7A0"/>
    <dbReference type="EnsemblPlants" id="KQK18888">
        <property type="protein sequence ID" value="KQK18888"/>
        <property type="gene ID" value="BRADI_1g45275v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18888">
        <property type="protein sequence ID" value="KQK18888"/>
        <property type="gene ID" value="BRADI_1g45275v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H7A0"/>
    <dbReference type="OrthoDB" id="638169at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163">
        <property type="entry name" value="OS02G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35163:SF12">
        <property type="entry name" value="OS05G0134500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="23"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK18888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="149" mass="17317" checksum="0AF70DB4D25986CB" modified="2016-01-20" version="1">MGISWPDSEESSEVSFPGEWELCEHGLTPFKRVAFEGVNTGRRFLTCPYEDERMCDWMCWVDDEHNEVLKNALDRLWWLLEDEKRKSSDQLAEKDWALGARYMAEKDKMELEKELAELKLKMKQSGGEAGTSGEMKKLGSEVEVKVKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HII8</accession>
    <name>A0A0Q3HII8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100821840</name>
        <name evidence="2" type="ORF">BRADI_4g16200v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
    <sequence length="421" mass="48614" checksum="E748A60E58D6AE9D" modified="2016-01-20" version="1">MEKEESNKPIMPPIETVELPELPRDILMNIFARLEIPDLVRASSVRPSWRSVYTSLLNLGKYKRSQTPCLLYTSESAGENVACLYSIAEKRVYKVSLPEPPICSRLLIGSSQGLLVTVDERSEMHIVNPITSEQVDLPSVITIEQVKPVYDDSGALRKYRYSRHTAKEVYSPPMIVELDELREMLHYKAFVFPNTTTGQYIVVLFHNPHRQLSFTRVGDDSWTWLPPRLHYEDCLYKDGLLYAVNYDGEIDVYDLSGPTVTMQIVLGMTDAITYSCMYIVQAPWGDLLQVWKSYKDYELHPEPGAFVFWNTGKFEIYEIDIERGERKKVKCLHDHALFLGHNQSLCISSVEYPALKRNHAYFTDDSYFWTRGFENNSRDIAILNLDNNIREELVSPQLWSDFPAPMWITLDVRAMDSALKK</sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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            <begin position="1"/>
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        </location>
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            <begin position="37"/>
            <end position="113"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Schmutz J."/>
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                <person name="Bevan M.W."/>
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                <consortium name="EnsemblPlants"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
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            <fullName evidence="7">Factor of DNA methylation 1-5/IDN2 domain-containing protein</fullName>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_2g03140v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02700">
        <property type="protein sequence ID" value="KQK02700"/>
        <property type="gene ID" value="BRADI_2g03140v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="1892195at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080188">
        <property type="term" value="P:gene silencing by siRNA-directed DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="XS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FDM1-5/IDN2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="XS_domain"/>
    </dbReference>
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        <property type="entry name" value="XS_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21596:SF49">
        <property type="entry name" value="FACTOR OF DNA METHYLATION 1-5_IDN2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03469">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="XS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="6"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Factor of DNA methylation 1-5/IDN2" evidence="3">
        <location>
            <begin position="334"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="136"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK02700.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="51984" checksum="CBEDA8E3471BDFC0" modified="2018-03-28" version="2">MADASEILVWPWTGILATEDDGGAAALAAHARQLPDAVATTALEEEAPPYRHCRGHHHFLVLHFGRSLQGLRAAASIAGLFPGAGRTEWRRRESSGPVYGWAAVEEDLRGEDAVGMFLRATGARGAAADAIAGELESAAEQETRRLRHELAELRAIADKVIPEMNRDLEDENEKLKAELDAASREIELKMEKIAQLENLDKDNGPTQGASEINTLDLAAGGECKVDDHALMLHENHKQEMEAIRTKLTQLEKQLAQREALVFTAQQLNMRVQAGEKLNEEDHQHLYAVVMSWWHILDEEQKRLKASCVNLIERERKNSKELQENRQELIQAFLFYFACKRKYSDDDPDGKAARLISSWQEEMKEPSWHPFTTIQVDEEDKEVIDDDDPKLKQLRIDYGDSVCYAVKVAMSELNENSPYGRHVFNELWNFREGRKATTAEVFMCILEQLTPANLGYISGMGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ISU3</accession>
    <name>A0A0Q3ISU3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g44600v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQK08891">
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        <property type="gene ID" value="BRADI_2g44600v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08891">
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        <property type="gene ID" value="BRADI_2g44600v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3ISU3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK08891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="115" mass="11039" checksum="5645E660E43F528D" modified="2016-01-20" version="1">MTARHGGDGAAVGGGSGGAGRRLGRDERAARSADGGAGTGAAARGRSGDVGATGKTRGGAARPGGDEARSGTVGDGAGDEDGRRRGHGSSEKSGQPGGAIGWGNKGERERRSRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IZF7</accession>
    <name>A0A0Q3IZF7_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">BRADI_4g38012v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IZF7"/>
    <dbReference type="EnsemblPlants" id="KQJ91487">
        <property type="protein sequence ID" value="KQJ91487"/>
        <property type="gene ID" value="BRADI_4g38012v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ91488">
        <property type="protein sequence ID" value="KQJ91488"/>
        <property type="gene ID" value="BRADI_4g38012v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT65166">
        <property type="protein sequence ID" value="PNT65166"/>
        <property type="gene ID" value="BRADI_4g38012v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ91487">
        <property type="protein sequence ID" value="KQJ91487"/>
        <property type="gene ID" value="BRADI_4g38012v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ91488">
        <property type="protein sequence ID" value="KQJ91488"/>
        <property type="gene ID" value="BRADI_4g38012v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65166">
        <property type="protein sequence ID" value="PNT65166"/>
        <property type="gene ID" value="BRADI_4g38012v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IZF7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ91488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="78" mass="8848" checksum="68638DB6956418AB" modified="2016-01-20" version="1">MSGLMIKQDITMASYVVWNIWKERNRGIFKGKSMSPFALSGLIREDRDPSAARKVYCTAELLVVISEVFVCFGFNLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J9Y0</accession>
    <name>A0A0Q3J9Y0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein LURP-one-related 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100837149</name>
        <name evidence="2" type="ORF">BRADI_1g18150v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562474.1">
        <property type="nucleotide sequence ID" value="XM_003562426.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J9Y0"/>
    <dbReference type="FunCoup" id="A0A0Q3J9Y0">
        <property type="interactions" value="1209"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3J9Y0"/>
    <dbReference type="EnsemblPlants" id="KQK14699">
        <property type="protein sequence ID" value="KQK14699"/>
        <property type="gene ID" value="BRADI_1g18150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837149"/>
    <dbReference type="Gramene" id="KQK14699">
        <property type="protein sequence ID" value="KQK14699"/>
        <property type="gene ID" value="BRADI_1g18150v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837149"/>
    <dbReference type="OrthoDB" id="652749at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF142">
        <property type="entry name" value="OS07G0672600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22428" checksum="44F839061496EF2C" modified="2016-01-20" version="1">MARVYSSSSSSSPVAAAMAASGQRREVFTVWMKSLVLNGRGCTVYDSGGRIVYRVDNYGSRSCHDVCLMDLHGGIVLNILKKKLAFGKWEGYKWSNDRKQQAWFTVARPPLFRRTSHQSSSSSCEFETEPGHTMRYRIDGGEKRACCRIVDEATGAVVAEVKRKVTAGGVALGEDVLALVVEPGVDHSLIMGLVLIYGLMNRTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JER6</accession>
    <name>A0A0Q3JER6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PHD finger protein ALFIN-LIKE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100830499</name>
        <name evidence="15" type="ORF">BRADI_2g57280v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the Alfin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564803.1">
        <property type="nucleotide sequence ID" value="XM_003564755.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JER6"/>
    <dbReference type="STRING" id="15368.A0A0Q3JER6"/>
    <dbReference type="EnsemblPlants" id="KQK10956">
        <property type="protein sequence ID" value="KQK10956"/>
        <property type="gene ID" value="BRADI_2g57280v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830499"/>
    <dbReference type="Gramene" id="KQK10956">
        <property type="protein sequence ID" value="KQK10956"/>
        <property type="gene ID" value="BRADI_2g57280v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830499"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JER6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15613">
        <property type="entry name" value="PHD_AL_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000306">
        <property type="entry name" value="PHD finger alfin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045104">
        <property type="entry name" value="Alfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021998">
        <property type="entry name" value="Alfin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044104">
        <property type="entry name" value="PHD_AL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321">
        <property type="entry name" value="CPG BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321:SF77">
        <property type="entry name" value="PHD FINGER PROTEIN ALFIN-LIKE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12165">
        <property type="entry name" value="Alfin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="203"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="147"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="185"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU369089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK10956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="263" mass="29492" checksum="2A4F76CAE3B71723" modified="2016-01-20" version="1">MEGAGGGAAYPSRTAEEVFRDLRGRRAGMIKALTEEVEKFYKLCDPEKENLCLYGYPNETWEVTLPAEEVPPEIPEPALGINFARDGMNGKDWLALVAVHSDSWLLAVAFYFGARFGFDRETRRRLFNMVNNLPTIYEVVTGVAKKQAKEKTPNSSSKSNKPTSKVQSRVEPRSSSKAKVSAPKDEEDSGDEDGDEVEEEHDNTLCGTCGTNDGKDEFWICCDNCEKWYHGKCVKITPARAEHIKHYRCPECTNGNSNKRLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JLF7</accession>
    <name>A0A0Q3JLF7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g06990v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12948.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JLF7"/>
    <dbReference type="FunCoup" id="A0A0Q3JLF7">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3JLF7"/>
    <dbReference type="EnsemblPlants" id="KQK12948">
        <property type="protein sequence ID" value="KQK12948"/>
        <property type="gene ID" value="BRADI_1g06990v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12948">
        <property type="protein sequence ID" value="KQK12948"/>
        <property type="gene ID" value="BRADI_1g06990v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JLF7"/>
    <dbReference type="OrthoDB" id="1916346at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JLF7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF13">
        <property type="entry name" value="F-BOX PROTEIN CPR30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK12948.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="48103" checksum="9C6CABABA8DE6E51" modified="2018-03-28" version="2" fragment="single">MDQTASAAMSCCDDASTSEDQQLACRQASRRRPRERVRCAPAPAPLADVVREHALLHLTPAAAARLRLVHPAWARRLASPLFAVAHAATPRRMSGLFVPTSGFLPFDSSDAVPSRTLAFAPASPGSITVLSSSHGLACCFSPADDAYFLCNPATASWAPVPCPPCRPTWPRPAMVVLFDASPYNFRGDYALVCAAEVAPGSGAYCFKVFTSGTGAWRVAAGDDAIVPAEGLVAASGVATGAGGMKTAWWRTSVGTAVGYSPATGRVEIVLCPGDSGMWEIGSAGGKLHCAVRDGSDVAVFRLIGEHGSCSWEEVTRVSVTELLPSRQVKLSESEGSGDDCELESASVAVVEEKEVRRLDDDGVRLLGFQGGAAWPEVVVLAGRRLVAFDTRTRRRREVTVPDPEEEEEQDEEQQRWDGAEYAVHTNTLALVAPAVLAGEPMLVDESLSAPPDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JQ70</accession>
    <name>A0A0Q3JQ70_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g51362v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JQ70"/>
    <dbReference type="EnsemblPlants" id="KQK00713">
        <property type="protein sequence ID" value="KQK00713"/>
        <property type="gene ID" value="BRADI_3g51362v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00713">
        <property type="protein sequence ID" value="KQK00713"/>
        <property type="gene ID" value="BRADI_3g51362v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JQ70"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036297566" description="Secreted protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK00713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12627" checksum="26CF0A20DD271F2D" modified="2016-01-20" version="1">MTLAVRLLCDPCFWSSLFFSFFLSQAPTCCPVPRGRKGGGGGWRRFRLPRRRLMVRSRRCRRGEERNRSVHPCPWIWFCSLVDVSFSVLFLPDLAGGARHACCSDFWLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JR69</accession>
    <name>A0A0Q3JR69_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g17116v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JR69"/>
    <dbReference type="EnsemblPlants" id="KQK14540">
        <property type="protein sequence ID" value="KQK14540"/>
        <property type="gene ID" value="BRADI_1g17116v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14540">
        <property type="protein sequence ID" value="KQK14540"/>
        <property type="gene ID" value="BRADI_1g17116v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JR69"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="141" mass="16443" checksum="27538A4568D47823" modified="2016-01-20" version="1">MELIIPRVRFQMEEVAPEVVVSMEERVVPVEEEEVLHTTTAIKVIRLSTAAAVNRNRRLLRRLRPPPSRPANVKCFARWIGLYPSSMYIFLILFNMSMWLFSVVVAIIVTTMIDLLVHLYLYESQLSDQMLLSVDRLRPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K1D0</accession>
    <name>A0A0Q3K1D0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100846792</name>
        <name evidence="14" type="ORF">BRADI_1g38025v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">May be a general defense protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563764.2">
        <property type="nucleotide sequence ID" value="XM_003563716.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A0Q3K1D0">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK18012">
        <property type="protein sequence ID" value="KQK18012"/>
        <property type="gene ID" value="BRADI_1g38025v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846792"/>
    <dbReference type="Gramene" id="KQK18012">
        <property type="protein sequence ID" value="KQK18012"/>
        <property type="gene ID" value="BRADI_1g38025v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846792"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K1D0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000179">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000059">
        <property type="entry name" value="ABC transporter G family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF28">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 39-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="580"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="620"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="690"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="720"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="778"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1254"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1284"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1340"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1367"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1398"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1429"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1478"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="178"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="903"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="818"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="833"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK18012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1509" mass="169865" checksum="D3E67965DA1FB9FA" modified="2016-01-20" version="1">MESAMEKVWDSGRRMSRSIGRGMGMETWGVDEAFLHGGGSRGRAGADDDEEALRWAAIERLPTYSRMRTAILSAEAAASAAADQGDGNKQQQQYKEVDVRKLGAGERQEFIERVFRVAEEDNQRFLQKLRDRIDRVGIELPTVEVRFERLTVEARCHVGSRALPTLLNTARNMAEGALGLLGARLGRQATLTILKDVSGVIRPSRMTLLLGPPSSGKTTLLLALAGKLDPTLACSGEVAYNGFPLEDFVPQKTAAYISQTDVHVGEMTVKETLDFSARCQGVGTKYDLLTELARREKEAGIRPEPEVDLFMKATSMEGVESSLQTDYTLRILGLDICADTIVGDQMQRGISGGQKKRVTTGEMIVGPTKVLFMDEISTGLDSSTTFQIVKCLQQIVHLGEATILMSLLQPAPEAFELFDDIILLSEGQIVYQGPREYVLEFFESCGFRCPERKGTADFLQEVTSKKDQEQYWADKQRPYRYISVSEFAQRFKRFHVGLQLENHLSVPFDKSRSHQAALVFSKHSVSTRELLKASFDKEWLLIKRNSFVYIFKTIQLIIVALIASTVFLRTQMHTRNLDDGFVYIGALLFTLIVNMFNGFAELSLTITRLPVFYKHRDLLFYPAWIFTLPNVVLRIPFSIIESVVWVVVTYYTMGFAPEADRFFKQLLLVFLIQQMAGGLFRAIAGLCRSMIIAQTGGALFLLIFFVLGGFLLPKDFIPKWWIWGYWISPLVYGYNALAVNEFYAPRWMNKFVMDKNGVPKRLGIAMLEGANIFTDKNWFWIGAAGLLGFSIFFNVLFTLSLAYLNPLGKPQAVISEETAKEAEGNGVPRDTVRNGSTKRNGSTKRTGSTKSGDGGNSNEIREVRLSSRLSNSSSNGIARVMSVGSNEAAPRRGMVLPFSPLSMCFDDVNYYVDMPAEMKQQGVTDDRLQLLREVTGSFRPGVLTALMGVSGAGKTTLMDVLAGRKTGGYIEGDIRISGYPKNQATFARISGYCEQNDIHSPQVTIRESLIYSAFLRLPENIGDEEITDDIKIQFVDEVMELVELDNLKDALVGLPGITGLSTEQRKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRTVRNTVDTGRTVVCTIHQPSIDIFEAFDELLLLKRGGQVIYSGKLGRNSQKMIEYFEAIPGVPKIKDKYNPATWMLEVSSVAAEVRLNMDFADYYKTSDLYKQNKVLVNRLSQPEPGTSDLHFPTAYSQSIIGQFKACLWKHWLTYWRSPDYNLVRFSFTLFTALLLGSIFWKIGTKMGDANTLRMVIGAMYTAVMFVGINNCATVQPIVSIERTVFYRERAAGMYAAMPYAIAQVVMEIPYVFVQASYYTLIVYAMMSFQWTAAKFFWFFFVSYFSFLYFTYYGMMTVSISPNHEVAAIFAAAFYSLFNLFSGFFIPRPRIPKWWIWYYWICPLAWTVYGLIVTQYGDMEEIISVPGQSNQTISYYVTHHFGYHRSFMAVVAPVLVLFAVFFAFMYALCIKKLNFQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K3Y3</accession>
    <name>A0A0Q3K3Y3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g46656v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K3Y3"/>
    <dbReference type="EnsemblPlants" id="KQK19156">
        <property type="protein sequence ID" value="KQK19156"/>
        <property type="gene ID" value="BRADI_1g46656v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19156">
        <property type="protein sequence ID" value="KQK19156"/>
        <property type="gene ID" value="BRADI_1g46656v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K3Y3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK19156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="102" mass="11149" checksum="AE951F57DCF55EFB" modified="2016-01-20" version="1">MYVGEEDGPVEAGRVSLHLCSSVSEFPSLPSGEFRKCKTLRHLLCAFAQPVHVQPKQCAGKENLMQFANKSLLEQSSMLVARACLPTKIVGLVIFCLITNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K4G0</accession>
    <name>A0A0Q3K4G0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UV radiation resistance-associated gene protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100829690</name>
        <name evidence="2" type="ORF">BRADI_2g21728v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566105.1">
        <property type="nucleotide sequence ID" value="XM_003566057.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K4G0"/>
    <dbReference type="STRING" id="15368.A0A0Q3K4G0"/>
    <dbReference type="EnsemblPlants" id="KQK05665">
        <property type="protein sequence ID" value="KQK05665"/>
        <property type="gene ID" value="BRADI_2g21728v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829690"/>
    <dbReference type="Gramene" id="KQK05665">
        <property type="protein sequence ID" value="KQK05665"/>
        <property type="gene ID" value="BRADI_2g21728v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829690"/>
    <dbReference type="OrthoDB" id="72772at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K4G0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157:SF22">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK05665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="40414" checksum="7BD93DE44C5F744C" modified="2016-01-20" version="1">MSSSTRTAAGPPDPGADHAVAAWPDFLGRLAELAAAAEARAALARRLEAALEVRRESLRQNAALDEMRRRLERKQRRAEEAVVGKRRAVEGVERRREQLQAQIERVLPLSRALAAAHRRVQEAKEELSGEKARLGDLQRLLRMRQQFMVAQVAALHPARIFHELTVAENHRSDANGEHGTMSEENEALPRKNGNGTHLLNVIKSPHVPHLTFFSWHIGKHKTKQQSYSHKELQRSAAVLGYAAHAVLLIASYLDVRLRYPLHFGGSRSYVSDRLPSDEAASVASAEHRGVNSNDSKLTDYPLFLEFQDDSTKASYAIYLLHKDTEQLLDYIGAESSERNVFGNLRELIRIIQSDEYLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KIH6</accession>
    <name>A0A0Q3KIH6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100824264</name>
        <name evidence="9" type="ORF">BRADI_1g77610v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230299.1">
        <property type="nucleotide sequence ID" value="XM_010231997.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KIH6"/>
    <dbReference type="STRING" id="15368.A0A0Q3KIH6"/>
    <dbReference type="EnsemblPlants" id="KQK24020">
        <property type="protein sequence ID" value="KQK24020"/>
        <property type="gene ID" value="BRADI_1g77610v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824264"/>
    <dbReference type="Gramene" id="KQK24020">
        <property type="protein sequence ID" value="KQK24020"/>
        <property type="gene ID" value="BRADI_1g77610v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824264"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KIH6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12203">
        <property type="entry name" value="GT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000061">
        <property type="entry name" value="Trihelix transcription factor GT-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000092">
        <property type="entry name" value="Trihelix transcription factor GT-2"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LIT7</accession>
    <name>A0A0Q3LIT7_BRADI</name>
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            <fullName evidence="2">VQ domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ORF">BRADI_4g43770v3</name>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
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    </dbReference>
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        <source>
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        </source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="189" mass="20041" checksum="DB177487F9F7882B" modified="2016-01-20" version="1">MLSAMMQQQQRTASASAYAYTPPPLPSASAWQGLPSPPSYEAPAAHQQQHRPRRAATKRRPRPSRRTPTTYIRAGPAEFRRMVHQVTGADDIDLLLNPPPPPPPQQQQAAAQPLRRLPCRPAPVPAAGALMLPTLDTSASFLLAGGGCRARTPAAAPTEAPPPPPASEGSLDLDDGGAGFPTLDSWDLL</sequence>
</entry>
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    <name>A0A0Q3LXK0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g28332v3</name>
    </gene>
    <organism evidence="1">
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LXK0"/>
    <dbReference type="EnsemblPlants" id="KQJ97021">
        <property type="protein sequence ID" value="KQJ97021"/>
        <property type="gene ID" value="BRADI_3g28332v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97021">
        <property type="protein sequence ID" value="KQJ97021"/>
        <property type="gene ID" value="BRADI_3g28332v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LXK0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ97021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="9655" checksum="6AE951FAFA781289" modified="2016-01-20" version="1">MEPPLPCLLPHVHLRTGSLAATPAWPPFLFSPHLGLYLRHPFQEGAVASAHHRFGTWEAAAGRDLAAAPTDELGFDIVQGQAMCSRKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NP91</accession>
    <name>A0A0Q3NP91_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_1g48002v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NP91"/>
    <dbReference type="FunCoup" id="A0A0Q3NP91">
        <property type="interactions" value="814"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3NP91"/>
    <dbReference type="EnsemblPlants" id="KQK19390">
        <property type="protein sequence ID" value="KQK19390"/>
        <property type="gene ID" value="BRADI_1g48002v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19390">
        <property type="protein sequence ID" value="KQK19390"/>
        <property type="gene ID" value="BRADI_1g48002v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NP91"/>
    <dbReference type="OrthoDB" id="582313at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056195">
        <property type="entry name" value="HTH_70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042:SF11">
        <property type="entry name" value="OS06G0173800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23671">
        <property type="entry name" value="HTH_70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="140"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK19390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="291" mass="31904" checksum="ECA208398D2CAD8C" modified="2016-01-20" version="1">MDPKLHQEWTSFEVQEARSLIARLSINMIDSYDADEKNKVHNHIVDALHGLFPWKTNEQVTDFYVTLYAEMHMMQGQEDSHAGGGGMHTACALPNHVNGSFGVPEEESGCANGAQGVCAMGDLAANSVFMVPEEEGEENVDGLRAFGRGDWKNISKYFVTTRTPVQVSSHAQKFFRRLKNRALSNTQRHSINDDVDPYYANTLTSESGSGAGQALTFTGLIHDPSIGLEAPTSSFVTLDNIAQLLYSQQVVQQPLWSEQQMMGSGAATMDGVGNFEPPCRQGSTYLPPRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PGX7</accession>
    <name>A0A0Q3PGX7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g19553v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PGX7"/>
    <dbReference type="EnsemblPlants" id="KQJ88561">
        <property type="protein sequence ID" value="KQJ88561"/>
        <property type="gene ID" value="BRADI_4g19553v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88561">
        <property type="protein sequence ID" value="KQJ88561"/>
        <property type="gene ID" value="BRADI_4g19553v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3PGX7"/>
    <dbReference type="OrthoDB" id="1210636at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF5">
        <property type="entry name" value="OS05G0421675 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ88561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="93" mass="10624" checksum="54306D4B344FB579" modified="2016-01-20" version="1">MWTCGWGTPPLKDQFPSLYRIATNHQAYVAEVMTVMPSNIAFGRNIVGTRLILWEELCANLVSVQLDDQPDSFSWMLSFAGSYFVKSVYDFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3SCD7</accession>
    <name>A0A0Q3SCD7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g68550v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3SCD7"/>
    <dbReference type="FunCoup" id="A0A0Q3SCD7">
        <property type="interactions" value="1454"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3SCD7"/>
    <dbReference type="EnsemblPlants" id="KQK22642">
        <property type="protein sequence ID" value="KQK22642"/>
        <property type="gene ID" value="BRADI_1g68550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22642">
        <property type="protein sequence ID" value="KQK22642"/>
        <property type="gene ID" value="BRADI_1g68550v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3SCD7"/>
    <dbReference type="OrthoDB" id="2382881at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3SCD7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038801">
        <property type="entry name" value="TAF1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15319">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15319:SF1">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="772"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="792" mass="87085" checksum="DF915BE285823BEB" modified="2016-01-20" version="1">MNLSEDWRFVFPVSSVFAPPSFAPSAAASRGPLLFSPAPSRTPLLSLPFPFPPPLTDASTTSGLRHALRSFVDSTSFLPSSDLDSLSEYLLAAPSTPFPAPSNLLAALRARSSGSLILFFPYGENADKVALKPVDLPLATTTTPVSLLFVQSDGFMHPGHRIQQLAAIPARSSWSPEPDHSCDEGFLLAATLYSVSWFCVKTQDSGSHMLVPVAKQGFDAAVVHACWNKHLPSQCAVLLESGELCWFDLDTRCGGKTRLGFGTDDDDCGHWLSCVYGAQPWVVIIASTKAVLLVDLRSVDSGEYKVIAKVGLPGLFETDPFVRTEHYLAFCKAGFDEFHISLVTERHLILLDVRQPLTPVLAWQHGLESPNHVTMFRLSELRPLEDYEWASNSGFAILVGSFSNGECGLFCYGPKEQGCPENSHLYAWDTPSWLSLTGQCYCSNRIMKEVFSTPASGYGNYASQHNADSIVGYYVLPNDLSMLEATSVGFTLVRLTASRKLEMQQYLAFRDLHDDTPCDESGHASRNITSSLSLNTEEENFSTRYRFLKLHFLSEHLKGKLCSALAKHDTSVNKQSGRLVISEDVSAFAEDNSKFSSRPVSDFLCNASVPMSIFEIACQSILNSLPSNILQVTFSQYKDMLSCTKEGLLEYLEVPSCPPSNKPRPFLLAKPSSTGEKLTSKVLTQNAFVGPVLPLHVLLAMELMSKGIGSLSEGETAETDLLSQQTSEILDAFVPEISIADADNCDGWSAPQKLNDKKSYFVYEPQIENKFTHDETARKKEKEKQKPSEDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGL3</accession>
    <name>A0A2K2CGL3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g11115v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
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            <position position="1"/>
        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="14423" checksum="9A8070902BF2F710" modified="2018-03-28" version="1" fragment="single">YTCVLCNGLQIETRDHLFFQCPFSLACWRYICPQFNPHNNVHHNVTDLKDHLDVPFPMEIIALGCWSIWKTRNDCIFNNIRPSLYRCRRIFKEELNLVFHRAKRNKYSNFKNWIDRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGY8</accession>
    <name>A0A2K2CGY8_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g13217v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CGY8"/>
    <dbReference type="EnsemblPlants" id="PNT61290">
        <property type="protein sequence ID" value="PNT61290"/>
        <property type="gene ID" value="BRADI_5g13217v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61290">
        <property type="protein sequence ID" value="PNT61290"/>
        <property type="gene ID" value="BRADI_5g13217v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CGY8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
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        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="435"/>
        </location>
    </feature>
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        <location>
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            <end position="498"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT61290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="563" mass="60101" checksum="D7EADAD8526C3D67" modified="2018-03-28" version="1">MSICPSFSFSCDNLSCNFSFCCGCDEDPAEEFPEEHCHVPYYYPPPPMPPPVQSMTNVITAPPPPQPQPPQAHYTTIVLPDQMSRWPTQPSHRAPPLVAPPHQAWLPPTEAPRFAPPPTVVPRHQESPPPTQAPRPALQPTVAPRYQVTQPPPPAPQPTVTPRHQVSPASIQASHPAPQPAVAPRHQLSPAPTQVPHPARAPTVAPHQHVTSPLTQASHPAPQPAVAPRHQLSPAPTQVPHPARPPNVAPHHHVPSLLTQSSHPAAPQPAVAPHHQLSPTPTQAPHPARAPTVAPRHHVPPLAQASHPTPPPTVVPHHQVSRQPTQGPHPSPPPMVAPQHQVSLPPTQAPRLAQLPTGVPPHQVLPLAAQGPLHATPPKGAPPPRQVSPTPTQAPRHAPPPMTAPPNQVLSSLTGAPRPEQPSTVKPPNQVSLPPTQTPRPTIPSMVTPPSQMSPQQQVTPSVTPLAREPPYKSHEPPTSIAVIKPTTMSSKSWTLTPQAPVPPYSVREPLAPIAGRLPSKRYNTSPGQVHETLAPTPIVATLPARVSSKRDEQTLPTVPYKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CH24</accession>
    <name>A0A2K2CH24_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100825675</name>
        <name evidence="4" type="ORF">BRADI_5g13760v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
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        <property type="protein sequence ID" value="PNT61320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003579950.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CH24"/>
    <dbReference type="STRING" id="15368.A0A2K2CH24"/>
    <dbReference type="EnsemblPlants" id="PNT61320">
        <property type="protein sequence ID" value="PNT61320"/>
        <property type="gene ID" value="BRADI_5g13760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825675"/>
    <dbReference type="Gramene" id="PNT61320">
        <property type="protein sequence ID" value="PNT61320"/>
        <property type="gene ID" value="BRADI_5g13760v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5545019at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CH24">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:ketoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
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    </dbReference>
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        <property type="entry name" value="very-long-chain 3-oxoacyl-CoA reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF22">
        <property type="entry name" value="B-KETO ACYL REDUCTASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SDRFAMILY"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT61320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="394" mass="42863" checksum="EA488548BDD11FA8" modified="2018-03-28" version="1">MLQRRSKVALQAFNLTKLQRQAAPPPTHPLPGLYKQPPLVALSSSLTHSFPLPNRLAVTSAFFRPQAHPHHMAGASAQPAWAQALAAVGLLIASRAATCLALWLYAAFLRPAKPLRRRYGAWAVVTGATDGIGRALAFELASAGLGLVLVGRSPDKLAAVASEIRTRSSSSSRPAEVRTFVIDFDDADGLAASVDALGESIRGLDVGVLVNNAGRCYPYARYFHEVSEELTRSLIRINVEAVTRVTHAVLQGMVERKRGAVVNIGSGAATILPSDPLYAVYAATKAYVDQFSRSLYVEYKNKGIDVQCQAPMYVATKMASIRKPSLFAPSPEDYARAAVRYIGYEPRCTPYWAHSLVWFLFSVLPESVADRYVLNMSLGIRHKGMAKDARKKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CN41</accession>
    <name>A0A2K2CN41_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BRADI_4g16183v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CN41"/>
    <dbReference type="STRING" id="15368.A0A2K2CN41"/>
    <dbReference type="EnsemblPlants" id="PNT63454">
        <property type="protein sequence ID" value="PNT63454"/>
        <property type="gene ID" value="BRADI_4g16183v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63454">
        <property type="protein sequence ID" value="PNT63454"/>
        <property type="gene ID" value="BRADI_4g16183v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CN41"/>
    <dbReference type="OrthoDB" id="1907415at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000649">
        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF90">
        <property type="entry name" value="OS11G0565920 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033311480" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="11">
        <location>
            <begin position="21"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="12">
        <location>
            <begin position="25"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="PNT63454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="411" mass="46836" checksum="3935596D2765EDD2" modified="2018-03-28" version="1">MHPNPTTKLLLLLLVLTTWCQPHRQERDALLAFKRGVRDLLGRLTSWRRGDDCCRWRGVRCSNRTGHVLGLRLGVGSEWYDDPFEATLSGQISLSLFSLEHLEHLDLGWNSGLHFPRGGHSPKLLSSLKNLRYLNLSGIRFERTLHPQLGNLSKLQYLDLSDTYSEKPRDLSWLTRLPLLCHLNMNGENLSKVHDWPHTVNMIPSLEVLSLSYCSLQNTSQQLPHLNLTKLEKLDLSGNDFHHLSESCWFWNLTSLKHLYLGYNNLYGQFPNTLGRMKSLQVFDFSYNNQMAIMTPNLLRNLCNLEVLNLRYSLSYGNMTELYESLRHSSKLRELDLRGNNIIGTIPAGIGQLTSLVTLDLSGNHLIGHVPSEISMLSSLTSLYLGDNNLTGVITEEHFSGLTSLKYMDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQ11</accession>
    <name>A0A2K2CQ11_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g24731v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQ11"/>
    <dbReference type="EnsemblPlants" id="PNT64125">
        <property type="protein sequence ID" value="PNT64125"/>
        <property type="gene ID" value="BRADI_4g24731v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64125">
        <property type="protein sequence ID" value="PNT64125"/>
        <property type="gene ID" value="BRADI_4g24731v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQ11"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT64125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="75" mass="8469" checksum="9D95F0C218D93039" modified="2018-03-28" version="1">MNHNANVINTAYHDAVKWYAMPSFSRNRDALLRHTFRSPASNSLKDPSTVVTTARTLFSSSSMTSSQRGRRPLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQL8</accession>
    <name>A0A2K2CQL8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g27734v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQL8"/>
    <dbReference type="EnsemblPlants" id="PNT64337">
        <property type="protein sequence ID" value="PNT64337"/>
        <property type="gene ID" value="BRADI_4g27734v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64337">
        <property type="protein sequence ID" value="PNT64337"/>
        <property type="gene ID" value="BRADI_4g27734v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQL8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT64337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="74" mass="8031" checksum="455E2748B8C42FE6" modified="2018-03-28" version="1">MYGHLILSAARRTVLNHAFCGTPEHCVLKGPSMITLLNNHGAEEAEREAAEGPDGAESQALHHPPLRRHASLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CS89</accession>
    <name>A0A2K2CS89_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100844623</name>
        <name evidence="10" type="ORF">BRADI_4g34737v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318801.1">
        <property type="nucleotide sequence ID" value="XM_024463033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CS89"/>
    <dbReference type="STRING" id="15368.A0A2K2CS89"/>
    <dbReference type="EnsemblPlants" id="PNT64900">
        <property type="protein sequence ID" value="PNT64900"/>
        <property type="gene ID" value="BRADI_4g34737v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844623"/>
    <dbReference type="Gramene" id="PNT64900">
        <property type="protein sequence ID" value="PNT64900"/>
        <property type="gene ID" value="BRADI_4g34737v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CS89">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044576579" description="GOLD domain-containing protein" evidence="8">
        <location>
            <begin position="26"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="33"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT64900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="225" mass="26230" checksum="B617906B1006B92A" modified="2018-03-28" version="1">MDGRSLRLIYFLVLCLMPLLRHAVAIRFVMDREECFSHNVDYEGDTVHVSFVVIKAETPWHYSEDGVDLVVKDPNGNQIHDSRAKTSDKFEFVVQKRGVHRFCFTNKSPYHETVDFDVHVGHFSYFDQHAKDEHFSPLFAQILKLDEALYSIQFEQHWLEAQTDRQAILQHLLEFLPYCDAVNENMSKRAVHKALFESAALIAASVVQIYLLRRLFERKLGSSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CUK7</accession>
    <name>A0A2K2CUK7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100831740</name>
        <name evidence="17" type="ORF">BRADI_3g01157v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233689.2">
        <property type="nucleotide sequence ID" value="XM_010235387.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CUK7"/>
    <dbReference type="EnsemblPlants" id="PNT65711">
        <property type="protein sequence ID" value="PNT65711"/>
        <property type="gene ID" value="BRADI_3g01157v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831740"/>
    <dbReference type="Gramene" id="PNT65711">
        <property type="protein sequence ID" value="PNT65711"/>
        <property type="gene ID" value="BRADI_3g01157v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CUK7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000628">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF429">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044576585" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="25"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="403"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="PNT65711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="766" mass="84319" checksum="B61220AB0E0048C9" modified="2018-03-28" version="1">MAGALPWLIFAATLLLASIKSSTASRMAKPGCIETCGNLTIPYPFGIGEGCFRERFDVSCENNTAYWGNSSINFKIYDISLLEGQITITTLIASSCFSNKKPGWVSLRTGRLFTVSTKANKLTAIGCNTFAILGGYNKHNFGAGCFSMCRDKKSVDESGQCSGMGCCQTSIPPKVIFSNITFDDRFSNSEASSFNPCSYAFVAKQDWFKFKPYYLDDDKFRKEFNDGAPTVLDWVAGYENCTEAVKNTSSYACISKNSQCINSLNATGYLCTCNNGFAGNPYLEGGCQDIDECSFPDQYRCHGICSNTIGSYTCNPIGAKLTKVSTGVVIGIGLGSAAGLIILFLTGCVISEKIKHRRTQMLKCKFYEQNRGQLLEQLVSQRADIAERMIIPLEELEKATNNFDKGREIGGGGHGTVYKGILSDLHVVAIKKPKKVVQREIDEFINEVAILSQINHRNVVKLYGCCLETEVPMLVYEFISNGTLYEHLHVDGPRSLPWNDRLRIAVETARSLAYLHSTASIPIIHRDVKSANILLDQALTAKVADFGASRFISVGKSGLTTMVQGTIGYLDPMYFYTGRLTERSDVYSYGVMLVELLTRKKPFSYLSPDGEGLVANFVALFEQGNLSGMLDPQVTDEGGEEVQEAAALAVACIKLRGEDRPSMRQVELTLEGLGPSKKHVSEDAGFNRLDKNNTITTNSPWSLRSSVEGSARQYTMEAELTMSSRNFSMCYASPYIHFCTTDGMPEEKAQKREEGLLPTKWRSATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CVH7</accession>
    <name>A0A2K2CVH7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nucleoid-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_3g06010v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the YbaB/EbfC family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CVH7"/>
    <dbReference type="FunCoup" id="A0A2K2CVH7">
        <property type="interactions" value="398"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66029">
        <property type="protein sequence ID" value="PNT66029"/>
        <property type="gene ID" value="BRADI_3g06010v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66030">
        <property type="protein sequence ID" value="PNT66030"/>
        <property type="gene ID" value="BRADI_3g06010v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66029">
        <property type="protein sequence ID" value="PNT66029"/>
        <property type="gene ID" value="BRADI_3g06010v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66030">
        <property type="protein sequence ID" value="PNT66030"/>
        <property type="gene ID" value="BRADI_3g06010v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CVH7"/>
    <dbReference type="OrthoDB" id="2020094at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CVH7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1310.10:FF:000004">
        <property type="entry name" value="Nucleoid-associated protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="entry name" value="Nucleoid-associated protein YbaB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33449">
        <property type="entry name" value="NUCLEOID-ASSOCIATED PROTEIN YBAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33449:SF1">
        <property type="entry name" value="NUCLEOID-ASSOCIATED PROTEIN YBAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="YbaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT66030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="26378" checksum="1FA20A51E6CB64C2" modified="2018-03-28" version="1">MARDRTEATEKGRKRIRWNSKGALRTRSDRPANMNPVACHRLQASAATPPFASRCRAAPQTPRSVSRLPPRASASTAGNRINVQGAFFLRCSTWKKASNRPFRVYSLFGGKKEKDENGDEAPSKGGIFGNIGNMQNLYETVKKAQMVVQVEAVRVQKELAATEIEGYCEGELIKVTLSGNQQPINVEITEAAMELGAEKVSELVNEAYKDAHQRSVQAMKERMADLAQSIGMPAGLGDGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CXF4</accession>
    <name>A0A2K2CXF4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF834 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g16051v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CXF4"/>
    <dbReference type="EnsemblPlants" id="PNT66713">
        <property type="protein sequence ID" value="PNT66713"/>
        <property type="gene ID" value="BRADI_3g16051v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66713">
        <property type="protein sequence ID" value="PNT66713"/>
        <property type="gene ID" value="BRADI_3g16051v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CXF4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="13840" checksum="95E96A2207698AFD" modified="2018-03-28" version="1">MLREVATDAATGGQPDLAGDASSGGHVCYHRMPTMLQLAATDVASGGRRHGWPDLAGSGGVERGGQADDAATSGHRCCDWRPAMLHWLPRMRRAAEGGAAGEISLDPGKRNAVGHAMLRPDAGDASTGDLGLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYM8</accession>
    <name>A0A2K2CYM8_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g21355v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT67129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CYM8"/>
    <dbReference type="EnsemblPlants" id="PNT67129">
        <property type="protein sequence ID" value="PNT67129"/>
        <property type="gene ID" value="BRADI_3g21355v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67129">
        <property type="protein sequence ID" value="PNT67129"/>
        <property type="gene ID" value="BRADI_3g21355v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CYM8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="99" mass="10753" checksum="18709AE54676A57E" modified="2018-03-28" version="1">MGLGGCRLAADELPVGDGLDVAVMDLNHLRVERHLIFKPEHIPGSATAFTHTCSSHLPAHRIVQPWGSSTGFPDHPSQLDAHPDHPNQPTYTHSHGISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D302</accession>
    <name>A0A2K2D302_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g43665v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
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                <person name="Mockler T.C."/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <strain evidence="8 9">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="8">Bd21</strain>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
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        <property type="gene ID" value="BRADI_3g49491v3"/>
    </dbReference>
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</entry>
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            <taxon>Poaceae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
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    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DAR8"/>
    <dbReference type="STRING" id="15368.A0A2K2DAR8"/>
    <dbReference type="EnsemblPlants" id="PNT71373">
        <property type="protein sequence ID" value="PNT71373"/>
        <property type="gene ID" value="BRADI_2g26746v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71373">
        <property type="protein sequence ID" value="PNT71373"/>
        <property type="gene ID" value="BRADI_2g26746v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DAR8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT71373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="81" mass="8784" checksum="92BA6DE722BC76E1" modified="2018-03-28" version="1">MVGNPLICGAAAEQDCYGTLPMPMSHGLNNTQDTLMPGKSKSHEAPIAFRCTTGCIDILLLFRGCYSGLLMEAHKRSTYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DCN9</accession>
    <name>A0A2K2DCN9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g38615v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DCN9"/>
    <dbReference type="EnsemblPlants" id="PNT72032">
        <property type="protein sequence ID" value="PNT72032"/>
        <property type="gene ID" value="BRADI_2g38615v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72032">
        <property type="protein sequence ID" value="PNT72032"/>
        <property type="gene ID" value="BRADI_2g38615v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DCN9"/>
    <dbReference type="OrthoDB" id="689430at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT72032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="84" mass="9761" checksum="002B75938066C062" modified="2018-03-28" version="1">MYILAVTELIQFLNLVGHTLSGWVDCSLARAKKFFVEGLGAIFWDIWRSRNATCFEKKVIHCPEAVFWCRERTTFLDFFVSLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DFS9</accession>
    <name>A0A2K2DFS9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100827656</name>
        <name evidence="9" type="ORF">BRADI_2g54100v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232515.1">
        <property type="nucleotide sequence ID" value="XM_010234213.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DFS9"/>
    <dbReference type="STRING" id="15368.A0A2K2DFS9"/>
    <dbReference type="EnsemblPlants" id="PNT73135">
        <property type="protein sequence ID" value="PNT73135"/>
        <property type="gene ID" value="BRADI_2g54100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827656"/>
    <dbReference type="Gramene" id="PNT73135">
        <property type="protein sequence ID" value="PNT73135"/>
        <property type="gene ID" value="BRADI_2g54100v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DFS9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14133">
        <property type="entry name" value="PKc_DYRK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000216">
        <property type="entry name" value="Putative serine/threonine-protein kinase dyrk2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000380">
        <property type="entry name" value="Serine/threonine-protein kinase ppk15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF113">
        <property type="entry name" value="HYPOTHETICAL SER-THR PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="425"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT73135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="731" mass="82016" checksum="41EBC0EBEBE4D2C9" modified="2018-03-28" version="1">MAAPGLDEVMAFLTEHGFASTASALRDDVLDRSADGESDSTATLGPQLPPLRLPASASGGAGGGVALEAPASPGSSSNSASSSAFVSMSSSPSEFDGVGLLNPYGVWSPPHSQSDGSSSEMEFGTARQYDTTDLFFQEGWLYDDHLFHSKPDDDGRDKEEDKFVHGGSGRTEMLVLNADDDRRHEHAGNNSCEGCAEVYTCSSPLCGCCGGELKNEEGLQVVSNSSSVVYGRYKVMDDQTEILDECGLDLFQLKQSVDSVLECDLPRDPGQGDEHLELNVVEKELQMLSSFDSYGDDDIVANPGFIPHVTDNIKLHDSTENNLESSSDKEYLKESYSLHPFPESGDPDDPYEFGDIGPLNTGVKSSTALIAEKEDMESNIDLALSNFQREYEVFELRIVHRKNRTGFEENKDFPIVLNSVIAGRYYVTEYLGSAAFSKVVQAHDLQTGIDVCLKIIKNDKDFFDQSLDEIKLLKFVNKYDPSDEHHVLRLYDYFYHQEHLFIVTELLRANLYEFQKYNQEFGGEVYFTLPRIQVIARQCLEALVYLHHLRIVHCDLKPENILIKSYRRCEIKVIDLGSSCFLTDNLCLYVQSRSYRAPEVILGLPYDQRIDIWSLGCILAELCTGEVLFPNEPLPMMLARMIGIIGPIDMEMLALGQEAHKYFTDDYDLFTKNEETDQLEYLIPEKSSLRRHLQCHDAEFVDFLSYLLQINPRKRPTASEALKHHWLSSEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGL9</accession>
    <name>A0A2K2DGL9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100837477</name>
        <name evidence="13" type="ORF">BRADI_2g58250v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase HD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGL9"/>
    <dbReference type="STRING" id="15368.A0A2K2DGL9"/>
    <dbReference type="EnsemblPlants" id="PNT73425">
        <property type="protein sequence ID" value="PNT73425"/>
        <property type="gene ID" value="BRADI_2g58250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73425">
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    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="297"/>
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            <begin position="113"/>
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        </location>
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            <begin position="283"/>
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        </location>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT73425"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="324" mass="35010" checksum="A4CD760EC7A0E95A" modified="2018-03-28" version="1">MSRSVYVNGSISSTANYFTGGVVVKPGETVKCDPGESHYHISQIALEADNMKENVEVFVNVGGKRIMLGTLSVDKHPQVVTDQVFDKEFELVHSSMTSNICFTGYQFKIVKSSNSSSKEGDDSDEEVPLAIPLDSNTDDYKNNQATHGVNKLTASRPADAPCSISKAAVQETDSLEGPEGDHKDKTAAQDPSDDNDSKGSDQDMDHTPKKMKGNKRPVETPLKTPSAKKTNIASPTVGKKTGSCVGKKSAHVHVATPYPAKQAHKISEHVHVATPHPAKHARKTPENSDKSKQSASYFCNSCNRTFNSSMGLEDHSRMKHGAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGX0</accession>
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        <name type="scientific">Brachypodium distachyon</name>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Bd21</strain>
        </source>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT73519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="498" mass="53873" checksum="E3FC8ACA3E85CF3F" modified="2018-03-28" version="1">MTRGPKKPLYIQCGALPKSMPIASIYLSVSIENESNQIMQQLASRARIAAAMRGGGAAAEGEGDLPEDVVLEILARVPGVADLFRCAAACRRWRALVSDRSFLRRRWPPGARHRSSLLGFFFNREDPAPATMAPAEENFVRAPRSVLRPGSPLLSTFVPGAGSAGILGRAAVVPLASHGGLLLVRLVDGTAVVDQEEGEEPAAAALDRLAVCDLNSGACHVLPPLHCGWFFDYFATSAYAVLPGSSADDSSAASSSFKVLVIGINEDKRQYYLRTCSSSSSSSSGCWSAPSKLFDPIEHGIFGPIKQRDAAVIVSVDQRGVRHYTAHWLLWDLAHFHALDVDAATGEASLHRLPVPPRDLPFHLYDMPRLAAAPDGAALSALCLLRNEGRLRVEVWTRRRRQGEDGGGELSWHHGRVVELQSRSKEMDGALCVCFGERSGTLLARDRERCLYVADLESRGGAIMEEVATELRVSGGCKTAIAVEIDWPALFACRLGRK</sequence>
</entry>
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    <accession>A0A2K2DHX4</accession>
    <name>A0A2K2DHX4_BRADI</name>
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        </submittedName>
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        <name evidence="2" type="ORF">BRADI_1g03483v3</name>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_1g03483v3"/>
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        <property type="gene ID" value="BRADI_1g03483v3"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT73880.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT73880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="49" mass="5333" checksum="3F08DD7FE446E871" modified="2018-03-28" version="1">MGNLAPYLPGRGRRGIKKPRGLGQGPTLHMVGALAYHLPKRESGRHNLC</sequence>
</entry>
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    <accession>A0A2K2DHZ3</accession>
    <name>A0A2K2DHZ3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g03954v3</name>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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</entry>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
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    </evidence>
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        <source>
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        <source>
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        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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</entry>
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            <strain evidence="2 3">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
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                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="2">Bd21</strain>
        </source>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </evidence>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DTJ7"/>
    <dbReference type="EnsemblPlants" id="PNT77604">
        <property type="protein sequence ID" value="PNT77604"/>
        <property type="gene ID" value="BRADI_1g65782v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77604">
        <property type="protein sequence ID" value="PNT77604"/>
        <property type="gene ID" value="BRADI_1g65782v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DTJ7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT77604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="80" mass="9230" checksum="9322007E0B8F3D70" modified="2018-03-28" version="1">MVRTKSPAKRKAVGVAIPKLPVYSRNCNSSWKEIVSSSKFSLVSGCRWKEQRRRWMQSTRNCSHWALLDAILEAFCDENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1GKK0</accession>
    <name>I1GKK0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100832494</name>
        <name evidence="11" type="ORF">BRADI_1g00750v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of plastid-encoded RNA polymerase (PEP) to specific initiation sites and are then released.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the sigma-70 factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK11976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558410.1">
        <property type="nucleotide sequence ID" value="XM_003558362.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GKK0"/>
    <dbReference type="STRING" id="15368.I1GKK0"/>
    <dbReference type="EnsemblPlants" id="KQK11976">
        <property type="protein sequence ID" value="KQK11976"/>
        <property type="gene ID" value="BRADI_1g00750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832494"/>
    <dbReference type="Gramene" id="KQK11976">
        <property type="protein sequence ID" value="KQK11976"/>
        <property type="gene ID" value="BRADI_1g00750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832494"/>
    <dbReference type="eggNOG" id="ENOG502QV7Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_11_0_1"/>
    <dbReference type="OMA" id="VQCGREP"/>
    <dbReference type="OrthoDB" id="206108at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903865">
        <property type="term" value="C:sigma factor antagonist complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06171">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016262">
        <property type="entry name" value="RNA_pol_sigma_SigB/C/D/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050239">
        <property type="entry name" value="Sigma-70_RNA_pol_init_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR RPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603:SF45">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR SIGF, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000767">
        <property type="entry name" value="RNA_pol_sigma_SigB/C/D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="Sigma2 domain of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA polymerase sigma-70 region 3" evidence="8">
        <location>
            <begin position="278"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase sigma-70 region 2" evidence="9">
        <location>
            <begin position="327"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase sigma-70 region 3" evidence="8">
        <location>
            <begin position="407"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase sigma-70 region 4" evidence="10">
        <location>
            <begin position="494"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK11976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="564" mass="63709" checksum="15CA49EB4A12EFE7" modified="2012-06-13" version="1">MNSSRSLLSSPLFPTSSPNFRSTASSPTPSRTSVPMMHDSTGRASTACHYSPSLVAEEQVHGSVSLKGEKALLELLLDMALEQHTQGKKVTGEEGAESEFESYLRDLQRQVIYQQVLGEEYNSTSVSTSTLSIKSSATLDLGATSATVLKEVAFLPEESGTSAAELDVTLLHRLDLNHSYEKLLSKGQVFIRSKRLLERRSKKRDAPRASSTDALCGVVSSTDALCGVVDTKKKEKSKKYGRVLDPDEPFKLFLRDPETTEFLTAKEEKQLFSEVQNLMKLEEAQRRLEAQCGREPAIEEWAKAVGMSCKDLQSSIHIGRRCREKMARSNFRLVIHVARKYQGYGLDIEDLVQDGCCGLMKTFEKFNPSKGCRFPTYAYWWIRQAIKKSIFKHSRLIRLPESVYALLKKVGKARLECILEGEQPTNENVARRAGITIEKLAKLKAKTRKSRSLQDRVWPDDGVTFQEITEDPNIEPPDLVVDRMMMRQQVRDFLGILSTREKEIIEHRFGIYDGEPKTLHVIGDMYGLSKERIRQLQNRALDKLKRSVSNQGFDVYLDLLTSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMT5</accession>
    <name>I1GMT5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100845125</name>
        <name evidence="10" type="ORF">BRADI_1g07150v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. May donate electrons to ubiquinone.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558700.1">
        <property type="nucleotide sequence ID" value="XM_003558652.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMT5"/>
    <dbReference type="STRING" id="15368.I1GMT5"/>
    <dbReference type="EnsemblPlants" id="KQK12973">
        <property type="protein sequence ID" value="KQK12973"/>
        <property type="gene ID" value="BRADI_1g07150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845125"/>
    <dbReference type="Gramene" id="KQK12973">
        <property type="protein sequence ID" value="KQK12973"/>
        <property type="gene ID" value="BRADI_1g07150v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845125"/>
    <dbReference type="eggNOG" id="KOG3256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067218_5_0_1"/>
    <dbReference type="OMA" id="QFFRAPY"/>
    <dbReference type="OrthoDB" id="204405at2759"/>
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        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
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    </dbReference>
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        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
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        <property type="entry name" value="NADH-quinone oxidoreductase subunit I 1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
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        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
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        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PRK05888.1-4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PRK05888.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF20">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010277"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK12973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="223" mass="25681" checksum="3696099128AD1520" modified="2012-06-13" version="1">MAAFLARQAALALRARQTAHLGPSATAMQGHLRTYMNAGAPKRFKEDEEKEQLAKELAKDWNAIFERSINTLFLTEMVRGLMLTLKYFFERKVTINYPFEKGPLSPRFRGEHALRRYDSGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYDKEKLLENGDRWETEIAENLKSEALYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1GN62</accession>
    <name>I1GN62_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal protein L13a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100845636</name>
        <name evidence="5" type="ORF">BRADI_1g08260v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559332.1">
        <property type="nucleotide sequence ID" value="XM_003559284.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GN62"/>
    <dbReference type="FunCoup" id="I1GN62">
        <property type="interactions" value="1622"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GN62"/>
    <dbReference type="EnsemblPlants" id="KQK13135">
        <property type="protein sequence ID" value="KQK13135"/>
        <property type="gene ID" value="BRADI_1g08260v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845636"/>
    <dbReference type="Gramene" id="KQK13135">
        <property type="protein sequence ID" value="KQK13135"/>
        <property type="gene ID" value="BRADI_1g08260v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845636"/>
    <dbReference type="eggNOG" id="KOG3204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076922_0_0_1"/>
    <dbReference type="OMA" id="TRFNKTH"/>
    <dbReference type="OrthoDB" id="737850at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3250:FF:000001">
        <property type="entry name" value="60S ribosomal protein L13a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000003">
        <property type="entry name" value="60S ribosomal protein L13a-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF39">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13X-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK13135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23575" checksum="A343958796A4267C" modified="2012-06-13" version="1">MVSGSGVCARRVVVDARHHMLGRLASIVAKELLNGQRVVVVRCEEICMSGGLVRQKMKYLRFLRKRMNTKPSHGPIHFRAPAKILWRTIRGMIPHKTKRGEAALARLKAYEGVPPPYDRTKRMVIPDALKVLRLQAGHKYCLLGQLSKEVGWNYADTIRELEEKRKEKAKVAYDRRKQLAKLRVKAEKTAEEKLGTQLEILAPIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I1GP01</accession>
    <name>I1GP01_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g10725v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GP01"/>
    <dbReference type="EnsemblPlants" id="PNT74235">
        <property type="protein sequence ID" value="PNT74235"/>
        <property type="gene ID" value="BRADI_1g10725v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74235">
        <property type="protein sequence ID" value="PNT74235"/>
        <property type="gene ID" value="BRADI_1g10725v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R4T7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1818462_0_0_1"/>
    <dbReference type="InParanoid" id="I1GP01"/>
    <dbReference type="OMA" id="NNMHITC"/>
    <dbReference type="OrthoDB" id="622517at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003641279" evidence="2">
        <location>
            <begin position="25"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT74235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="154" mass="15503" checksum="FA406B4E82DD6F50" modified="2012-06-13" version="1">MARYARALLPLLLLACALVRSSHCSRSPPPGEPQKPGRVVVPSHSPVAHGGADDDEPRGGETATTGQVRLVEKEAAGANAGYGAAASSAFGNGGVGSEQRGAAALLVQQRAPLRRMMLSSSGRVQGGDAEDSAAAGASCRSNNAHITCAPPAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPF9</accession>
    <name>I1GPF9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BAG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100821972</name>
        <name evidence="4" type="ORF">BRADI_1g12090v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559560.1">
        <property type="nucleotide sequence ID" value="XM_003559512.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPF9"/>
    <dbReference type="FunCoup" id="I1GPF9">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GPF9"/>
    <dbReference type="EnsemblPlants" id="KQK13727">
        <property type="protein sequence ID" value="KQK13727"/>
        <property type="gene ID" value="BRADI_1g12090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821972"/>
    <dbReference type="Gramene" id="KQK13727">
        <property type="protein sequence ID" value="KQK13727"/>
        <property type="gene ID" value="BRADI_1g12090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821972"/>
    <dbReference type="eggNOG" id="ENOG502RY04">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059259_0_0_1"/>
    <dbReference type="OMA" id="SRRFFGY"/>
    <dbReference type="OrthoDB" id="696633at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040400">
        <property type="entry name" value="BAG5/6/7/8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33322">
        <property type="entry name" value="BAG DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33322:SF4">
        <property type="entry name" value="BAG DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="3">
        <location>
            <begin position="179"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="353"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK13727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="453" mass="49701" checksum="276AF2AD3BF171D1" modified="2012-06-13" version="1">MASRRFFTYDPYDYYYPTPYGHPYSYYQHPSAARSPARNDGLFYPHIQRTEPAVRAAARRSPYDLFPDVESTEAPMGASGYPDIYYNQRPSASARAPARNNGALFPDVDCAEPAAKEARRSVSVPVHFAGPELGPERERETAVATEMAIPKKQAPSAEQAAVRLQASARGFLARRMVREVRAVEREAEAVAARIAAEAEALLANTRARVAIGEELMRLLLRLDAVRGVREYRRRVTKRVLALQDAVDALEIKPAPAPVEMSEESGMANQAAEQGVRIEPEEEGVPIESDDAVDSPATETIAEMDMVVDGGIATSDERDLGEAELMMDGEKAEEAEGENAEEAEGEWEMVAEEEAVAGENPAPKEQQQEPAAEEKEKKAEGGGATDGLDARKVMEMVAAMCEQSAQQCAAIGALAERVDALERAVRRVEVADRRRRRNKKLKKDGKAARCCYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPY9</accession>
    <name>I1GPY9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836611</name>
        <name evidence="3" type="ORF">BRADI_1g13610v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561793.1">
        <property type="nucleotide sequence ID" value="XM_003561745.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPY9"/>
    <dbReference type="FunCoup" id="I1GPY9">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GPY9"/>
    <dbReference type="EnsemblPlants" id="KQK13963">
        <property type="protein sequence ID" value="KQK13963"/>
        <property type="gene ID" value="BRADI_1g13610v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836611"/>
    <dbReference type="Gramene" id="KQK13963">
        <property type="protein sequence ID" value="KQK13963"/>
        <property type="gene ID" value="BRADI_1g13610v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836611"/>
    <dbReference type="eggNOG" id="KOG2824">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068996_0_0_1"/>
    <dbReference type="OMA" id="CPCEGCA"/>
    <dbReference type="OrthoDB" id="423313at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03031">
        <property type="entry name" value="GRX_GRX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45669">
        <property type="entry name" value="GLUTAREDOXIN DOMAIN-CONTAINING CYSTEINE-RICH PROTEIN CG12206-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45669:SF16">
        <property type="entry name" value="GLUTAREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23733">
        <property type="entry name" value="GRXCR1-2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="2">
        <location>
            <begin position="190"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK13963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="345" mass="36722" checksum="37F5743FE8FCB180" modified="2012-06-13" version="1">MRGLRSRILRTLQSFPNAAAAAAVQSNILLLPPPDAFTTSSPPEKCRLQEHAPSPMAAGADQAVPELPTVSDGDDDDKENVSPEANPRKAKKMKVSSDHCDHDGSAVVDPGLAAAASAKCYRRPDLASATLFDPDLLAEFRGVVDAYARAFEKTKRSHDEEGDDDAVLLDGMDPLAGFESRCPPGGERAVVLYTTSLRGVRKTFEDCATVRRLLDGLRVAFLERDVSMHAPYRDELRALLLPPDSAAMAMPLPPRLFVDGRYVGGADEVVALHERSGLRPMLRGAPRRAAGEAACAVCGGDWFVVCGGCSGRHWLYDDGGGGGSANRVPCPGCNENGLVPCPLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQZ8</accession>
    <name>I1GQZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100821473</name>
        <name evidence="16" type="ORF">BRADI_1g17250v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562424.1">
        <property type="nucleotide sequence ID" value="XM_003562376.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQZ8"/>
    <dbReference type="STRING" id="15368.I1GQZ8"/>
    <dbReference type="EnsemblPlants" id="KQK14558">
        <property type="protein sequence ID" value="KQK14558"/>
        <property type="gene ID" value="BRADI_1g17250v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK14559">
        <property type="protein sequence ID" value="KQK14559"/>
        <property type="gene ID" value="BRADI_1g17250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821473"/>
    <dbReference type="Gramene" id="KQK14558">
        <property type="protein sequence ID" value="KQK14558"/>
        <property type="gene ID" value="BRADI_1g17250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14559">
        <property type="protein sequence ID" value="KQK14559"/>
        <property type="gene ID" value="BRADI_1g17250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821473"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_59_0_1"/>
    <dbReference type="OMA" id="KNYRMRM"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14663">
        <property type="entry name" value="STKc_SnRK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000279">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000096">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000002">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF168">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="19"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="NAF" evidence="15">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK14558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="448" mass="50647" checksum="6612A832F4D9641E" modified="2012-06-13" version="1">MYKAKRAASLKVRRRIGKYELGRTIGEGTFAKVRFAKNMESGDPVAIKILDKAKVQKHGLVEQIRREICTMKLIQHPNVVRLHEVMGSKARIFIVLEYVTGGELHDIIVARGSLKEDEARRYFQQLINAVDYCHSRGVYHRDLKLENLLLDSAGNLKISDFGLSAISEQVKDDGLLYTSCGTPNYVAPEVIEDKGYDGTLADLWSCGVILFVMLAGYLPFEDDNIASLYKMISGAQFTCPSWFSDGAKRLIARILDPNPSTRITIPQLLKDPWFKKGYKPPVFDEKYQTSLDDVDAAFGDSEEKHVKEEMEGQPASMNAFELISLNRGLNLENLFETDKKYKREKRFTSQCPPKEIINRIEAAAKPLGFDIQKKNYKMQMENIEAGRKGNLNVATEVFQVAPSLHVVVLKKARGDTLEFQKFYRTLSAQLKDVVWVCEGEAEEKSSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1GR49</accession>
    <name>I1GR49_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cullin-associated NEDD8-dissociated protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cullin-associated and neddylation-dissociated protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100829264</name>
        <name evidence="8" type="ORF">BRADI_1g17710v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CAND family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562449.1">
        <property type="nucleotide sequence ID" value="XM_003562401.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GR49"/>
    <dbReference type="STRING" id="15368.I1GR49"/>
    <dbReference type="EnsemblPlants" id="KQK14632">
        <property type="protein sequence ID" value="KQK14632"/>
        <property type="gene ID" value="BRADI_1g17710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829264"/>
    <dbReference type="Gramene" id="KQK14632">
        <property type="protein sequence ID" value="KQK14632"/>
        <property type="gene ID" value="BRADI_1g17710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829264"/>
    <dbReference type="eggNOG" id="KOG1824">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007157_0_0_1"/>
    <dbReference type="OMA" id="AYIPHFQ"/>
    <dbReference type="OrthoDB" id="6260732at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GR49">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010265">
        <property type="term" value="P:SCF complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000242">
        <property type="entry name" value="Cullin-associated NEDD8-dissociated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039852">
        <property type="entry name" value="CAND1/CAND2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013932">
        <property type="entry name" value="TATA-bd_TIP120"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12696">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08623">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25782">
        <property type="entry name" value="TPR_CAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="TATA-binding protein interacting (TIP20)" evidence="7">
        <location>
            <begin position="1040"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="309"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK14632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1219" mass="133872" checksum="FFDEA34D24AAEBAF" modified="2012-06-13" version="1">MANMNITGILEKMTGKDKDYRYMATSDLLSELNKESFKADQDLEPKLTNIVLQQLEDASGDVSGLAVKCLAPLVKKVGEERVVEMGNKLCDKLLNGKEQHRDTASIALKTIIVEVTTTSLAEKILISIAPQLINGVTSGKSAEIKCECLDILGDVLHRFGNLITKNHASMLTALLSQLNCNQASVRKKSVSCIASLAPCLSDDLLAKATLHVVQLLKNRSAKSEIARTNIQMIGALSRSVGYRFGPHLGEAVPLLINYCTSASENDEELREYSLQALESFVLRCPRDISPYCEGILNLSLEYISYDPNFTDSMEEDTDDEGHDEEDDDESANEYTDDEDASWKVRRASAKCLSAIIVSRPQMLSKMYQEACPKLIERFREREENVKMDIFNTFIELLRQTGNVTKGQGDIDESSPRWLLKQEVPKVVKSINRQLREKSVKTKVGAFSVLKELVVVLPDCLADLFGSLVPGIEKALNDKSSTSNLKIEALAFLRIVMASHSPSVFHPYIKALSGPILSAIGDRYYKVTAEALRVCGELVRVLRPNFEAHSLDFRPYIGPIYNAILARLANQDQDQEVKECAISCMSLVISTFGDGLQRELPSCLPILVDRMGNEITRLTAVKAFAVIANSPLRIDLSCVLDHVVSELTAFLRKANRALRQATLGTLNSLVVTYGGQIGSSSYETILAELSTLISDMDLHMAALALELCCTIIVDRKSIQNVGLAVRSKVLPQALILIRSALLQGQALQALQKFFASLVQSANTSFDTLLDSLISTAKPSQSGGLAKQALSSIAQCVAVLCLAAGDQKCASTIEMLKGILKDDSSTNSAKQHMALLCLGEIGRRKDLSNHVQIENIVIESFQSPFEEIKSAASYALGNIAVGNLSKYLPFILDQIDNQQKKQYLLLHSLKEVIARQSVDHTGQSELQDSNIVKILALLFNHCESEEEGVRNVVAECLGKIALIEPQKLIPALKERTCSPAANTRATVAIAIKYSIVERAGKIDEIMCSEISTFLMLIKDSDRHVRRAAVLALSTAAHNKPSLIKGLLPELLPLLYDQTVVKQELIRTVDLGPFKHVVDDGLELRKAAFECVDTLLDTCLDQVNPSSFIVPFLLSGLGDHYDVKMPCHLILSKLADKCPSAVLAVLDSLVEPIEKTISHKPKGDAVKQEVDRNEDMIRSALRAIAALSRLSGSDYSLKMKNLMNKITATPSLAEKYNSVRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRJ2</accession>
    <name>I1GRJ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100826945</name>
        <name evidence="15" type="ORF">BRADI_1g18930v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562524.1">
        <property type="nucleotide sequence ID" value="XM_003562476.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRJ2"/>
    <dbReference type="FunCoup" id="I1GRJ2">
        <property type="interactions" value="2034"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GRJ2"/>
    <dbReference type="EnsemblPlants" id="KQK14841">
        <property type="protein sequence ID" value="KQK14841"/>
        <property type="gene ID" value="BRADI_1g18930v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826945"/>
    <dbReference type="Gramene" id="KQK14841">
        <property type="protein sequence ID" value="KQK14841"/>
        <property type="gene ID" value="BRADI_1g18930v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826945"/>
    <dbReference type="eggNOG" id="KOG0330">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_1_1"/>
    <dbReference type="OMA" id="GIGIKCC"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17954">
        <property type="entry name" value="DEADc_DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044765">
        <property type="entry name" value="DDX47/Rrp3_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="18"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="49"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="247"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="406"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="18"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK14841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="49988" checksum="1D9F1AAC18023431" modified="2012-06-13" version="1">MAVEREPELEPEPVAEASTFAELGICKELVDACDSMGWKEPTKIQAGAIPHALQGRDLIGLGQTGSGKTGAFALPIIQALLEHRQPFFACVMSPTRELAIQIAEQFEALGSAIGLVCSVLVGGVDRMQQVLSIAKRPHIVVGTPGRLLDHLKDTKGFSLNKVKYLVLDEADKLLNLEFKESLDDILKAIPKERRTYLFSATMTKKVAKLQRACLRNPVKVEVSSKYSTVDTLKQEWYFVPAAYKDCYLVHALNELPGSMIMIFVRTCESTRLLALTLRNLGFKALSISGQMSQDKRLGALNKFKAKDFNILICTDVASRGLDIQGVDVVINYDIPMNSKDYVHRVGRTARAGKSGYAVSLVNQYEGQWFKMIEALLGKEIDLRKVDSDEIMILREHISDSRRIALTKLKEDGGHKKRRKQDDDDDEEEEKAPRGHRKPKSFKKSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSG7</accession>
    <name>I1GSG7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100846045</name>
        <name evidence="2" type="ORF">BRADI_1g21780v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235769.1">
        <property type="nucleotide sequence ID" value="XM_010237467.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSG7"/>
    <dbReference type="STRING" id="15368.I1GSG7"/>
    <dbReference type="EnsemblPlants" id="KQK15302">
        <property type="protein sequence ID" value="KQK15302"/>
        <property type="gene ID" value="BRADI_1g21780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846045"/>
    <dbReference type="Gramene" id="KQK15302">
        <property type="protein sequence ID" value="KQK15302"/>
        <property type="gene ID" value="BRADI_1g21780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846045"/>
    <dbReference type="eggNOG" id="ENOG502RZMV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111746_1_0_1"/>
    <dbReference type="OMA" id="FIKNDVP"/>
    <dbReference type="OrthoDB" id="5873279at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009579">
        <property type="term" value="C:thylakoid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021374">
        <property type="entry name" value="DUF2996"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36341">
        <property type="entry name" value="DUF2996 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36341:SF3">
        <property type="entry name" value="DUF2996 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11210">
        <property type="entry name" value="DUF2996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK15302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="218" mass="23589" checksum="48A972B8C428ABA6" modified="2012-06-13" version="1">MAPQALRLLRLTTPLGPRGGHHAPPLFAKRLPLVVAFPRGAGASCSAVQESSAAAAATTVSEKKDADGDNKEAAAEAKPAAKPAAAAAKLKKPPPKPLPEMMEEEIIPPLKAALEGEEDVSQVELSFQNNTLEGSFQKKDIPYYFWAFFPQGDLTGPKGFAMTSYSMEVSTIEPFLVDEKRVTPQYVVFWVYKRLAGQGILPVWKEEDLNPEPSTVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I1GUR2</accession>
    <name>I1GUR2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g28620v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GUR2"/>
    <dbReference type="EnsemblPlants" id="KQK16409">
        <property type="protein sequence ID" value="KQK16409"/>
        <property type="gene ID" value="BRADI_1g28620v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16409">
        <property type="protein sequence ID" value="KQK16409"/>
        <property type="gene ID" value="BRADI_1g28620v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S74I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129534_1_0_1"/>
    <dbReference type="InParanoid" id="I1GUR2"/>
    <dbReference type="OMA" id="YCCACAR"/>
    <dbReference type="OrthoDB" id="661559at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33181">
        <property type="entry name" value="OS01G0778500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33181:SF25">
        <property type="entry name" value="OS07G0115500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK16409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="105" mass="11727" checksum="74E74EA1E5873C0B" modified="2012-06-13" version="1">MSCLGRAAVPVKRVWLGLSGRLGLRRTTGRAGLGELRKEVRTCEYRDVHVMWEMLGGMDEAPPLPWLGDNKKNKPTASATRRRRSRKAAAPAWSRLVSYCCACAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWP6</accession>
    <name>I1GWP6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">HKT24 transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100846381</name>
        <name evidence="9" type="ORF">BRADI_1g34210v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrkH potassium transport family. HKT (TC 2.A.38.3) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563514.1">
        <property type="nucleotide sequence ID" value="XM_003563466.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWP6"/>
    <dbReference type="STRING" id="15368.I1GWP6"/>
    <dbReference type="EnsemblPlants" id="KQK17401">
        <property type="protein sequence ID" value="KQK17401"/>
        <property type="gene ID" value="BRADI_1g34210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846381"/>
    <dbReference type="Gramene" id="KQK17401">
        <property type="protein sequence ID" value="KQK17401"/>
        <property type="gene ID" value="BRADI_1g34210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846381"/>
    <dbReference type="eggNOG" id="KOG1341">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008384_2_0_1"/>
    <dbReference type="OMA" id="FLAMMCI"/>
    <dbReference type="OrthoDB" id="9999863at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GWP6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF11">
        <property type="entry name" value="CATION TRANSPORTER HKT2_3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK17401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="56012" checksum="3990C74AD068F400" modified="2012-06-13" version="1">MPSRLHIFVSSARHVTNSSVFISRFIASHLSPLLIHLSYFVIFDILGFVALMALKPSNPNYTPRYIDMLFLSTSALTVTGLATIKMEDLSSSQILVLTLLMLLGSEMFVSLLGLVHELSKQNKHDPEDSRVRSVTVLNESQVEEAIPATPSTNSNGLKKSCLKYLGFVLVAYMVTILLVGSLLVFMYVTHVSTARDVLARKSINTVLFSISVTTSSFTNGGLLPTNESMAVFASNQGLLLLLTGQILAGSMLLPVILRLVIWALKGLRMTKVKPEEFEFMMHNTKAMGLNHLLPNLQTVFLAVTVTALTAVAVTLFCCLNWYSAVFTGLTSSQKITNALFMAVNARQAGENSIDCSLVAPAALLLFMAMMYIPASTTVFSVQDDDARGDTEHKDGPKKRSLSLNNMLFSPLACNAAMIMIVCITERRLLFTDPLNFSTFNMIFEVISAYGNVGLSIGYSCSRLLHPEKESICHEKPYSFSGWWSDQGKVVLVLVMLYGRLKCFHKHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXB3</accession>
    <name>I1GXB3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Alpha-L-arabinofuranosidase B arabinose-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100829886</name>
        <name evidence="4" type="ORF">BRADI_1g36027v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563628.2">
        <property type="nucleotide sequence ID" value="XM_003563580.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXB3"/>
    <dbReference type="EnsemblPlants" id="KQK17676">
        <property type="protein sequence ID" value="KQK17676"/>
        <property type="gene ID" value="BRADI_1g36027v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829886"/>
    <dbReference type="Gramene" id="KQK17676">
        <property type="protein sequence ID" value="KQK17676"/>
        <property type="gene ID" value="BRADI_1g36027v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829886"/>
    <dbReference type="eggNOG" id="ENOG502QRCI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008033_0_0_1"/>
    <dbReference type="OMA" id="HVGCRTR"/>
    <dbReference type="OrthoDB" id="5358475at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012878">
        <property type="entry name" value="Beta-AFase-like_GH127_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049046">
        <property type="entry name" value="Beta-AFase-like_GH127_middle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31151">
        <property type="entry name" value="PROLINE-TRNA LIGASE (DUF1680)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31151:SF0">
        <property type="entry name" value="PROLINE-TRNA LIGASE (DUF1680)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07944">
        <property type="entry name" value="Beta-AFase-like_GH127_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20736">
        <property type="entry name" value="Glyco_hydro127M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094142" description="Alpha-L-arabinofuranosidase B arabinose-binding domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="Non-reducing end beta-L-arabinofuranosidase-like GH127 catalytic" evidence="2">
        <location>
            <begin position="132"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Non-reducing end beta-L-arabinofuranosidase-like GH127 middle" evidence="3">
        <location>
            <begin position="524"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK17676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="874" mass="94713" checksum="5F6FFA35EE6C5EE4" modified="2012-06-13" version="1">MARRGLPLPAPLVMPALSLALALAMAVAKECTNVPTQLSSHTVRARLQGDPSAEEWRLRALFHDHAHVSPTDEATWMDLRAPLASSAATEESGWAMLYRALKGSASGGSASAAAGFLEEVPLQDVRLDMEEDAVYGRAQQTNLEYLLLLDVDRLLWSFRTQAGLPAPGKPYGGWEGADVELRGHFVGHYLSAAAKTWASTHNGTLAAKMSAVVDALHECQQAAAANGGNGYLSAFPAEFFDRFEAIQPVWAPYYTVHKIMQGLLDQHTVAGNGKALAMAVAMAGYFGGRVRSVIQRHGIERHWTSLNEETGGMNDVLYQLYTITNDQRHLVLAHLFDKPCFLGLLAVQADSLTGFHANTHIPVVVGGQMRYEVTGDPLYKEISTFFMDIVNTSHSYATGGTSVSEFWSDPKRLASTLTTENEESCTTYNMLKVSRHLFRWTKEIAYADYYERALINGVLSIQRGRDPGVMIYMLPQGPGRSKAVSYHGWGTQYDSFWCCYGTGIESFSKLGDTIYFEEKGSKPTLYVVQYIPSIFNWKSAGLTVTQRLKPLSSSDQYLQVSLSISAKTNGQYATVNVRIPSWASANGAKATLNDKYLQLGSPGTFLTVTKQWNSGDHLTLQLPINLRTEAIKDDRAEFASLQAVLFGPFLLAGLSTGDWDAKTGAAAAAISDWISPVPSSYSSQLVTLTQESGGSTFVLSTVNGTSLAMQPRPEGGGTEAAVHGTFRLVPQGFSPPPTTNRRHGAPTNLASAMIEPFDLPGMAITDALTVVRSEEKSSGSLLFNVVPGLDGKPGSVSLELGTRPGCFVVTAGAKVQVGCGAGFSQAAASFARAEPLRRYHPISFVARGARRGFLLEPLFTLRDEFYTVYFNLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZB0</accession>
    <name>I1GZB0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">U-box domain-containing protein</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase PUB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100845469</name>
        <name evidence="7" type="ORF">BRADI_1g44390v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564002.1">
        <property type="nucleotide sequence ID" value="XM_003563954.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZB0"/>
    <dbReference type="FunCoup" id="I1GZB0">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK18739">
        <property type="protein sequence ID" value="KQK18739"/>
        <property type="gene ID" value="BRADI_1g44390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845469"/>
    <dbReference type="Gramene" id="KQK18739">
        <property type="protein sequence ID" value="KQK18739"/>
        <property type="gene ID" value="BRADI_1g44390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845469"/>
    <dbReference type="eggNOG" id="ENOG502QS2D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006348_1_3_1"/>
    <dbReference type="OMA" id="NARCPVT"/>
    <dbReference type="OrthoDB" id="10064100at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000442">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045185">
        <property type="entry name" value="PUB22/23/24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22849:SF16">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22849">
        <property type="entry name" value="WDSAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="6">
        <location>
            <begin position="40"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK18739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="449" mass="47200" checksum="7DD7AD0B42A51295" modified="2012-06-13" version="1">MVLPMSRATRLMPELPLLRRGRRQAITASTSEDHQEEELAIPAHFRCPISLDLMRDPVTAPTGITYDRENLEGWLARGHGTCPVTGRGPLRLADLVPNHATRRMIQAWCVANRARGVERVPTPKVPVAEADAAQVLEDLSAAARLGDAAACGEIAARARALGKESDRNRRCLASAGAARKLSSAFGRLAAAGGEPVEGGALGKVLAALTVFFPLDDESRRCIVASSLTTLVSVLSHGDLAARASAAIVLREVASSAADRATVEAISRAPGMCDALVGLVRNPVSPQATKAALVTAYYLASDRAAASRFAELGVVSVLAELLVDADKGTSEKALAALDGVLCADAGLESARAHALVVPVLVKKMFRVSDMATEFAVSALWRLCHAGADAGACRVEALRVGAFQKLLLLLQVGCGGPTKERASELLKLLNGSRASVECIEAVDFKGLKRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1H0B0</accession>
    <name>I1H0B0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein CDI</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100821670</name>
        <name evidence="2" type="ORF">BRADI_1g47210v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560986.1">
        <property type="nucleotide sequence ID" value="XM_003560938.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H0B0"/>
    <dbReference type="EnsemblPlants" id="KQK19255">
        <property type="protein sequence ID" value="KQK19255"/>
        <property type="gene ID" value="BRADI_1g47210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821670"/>
    <dbReference type="Gramene" id="KQK19255">
        <property type="protein sequence ID" value="KQK19255"/>
        <property type="gene ID" value="BRADI_1g47210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821670"/>
    <dbReference type="eggNOG" id="ENOG502QPHU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1039721_0_0_1"/>
    <dbReference type="OMA" id="FMDCDML"/>
    <dbReference type="OrthoDB" id="590152at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35105">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35105:SF2">
        <property type="entry name" value="PROTEIN CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK19255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="31417" checksum="5FD5EF961439FAA1" modified="2012-06-13" version="1">MSPPAQAPTNNLTGAGAGTDEPFRVFVGYDSREDIAYRVCRRSLLRRSSIPLEIIPIVQDELRSAGLYWRERGPTESTEFSFTRFLTPHLAGYRGWALFIDCDFLFVADVAELARMCAAANPRHAVLCVHHDYAPKEATKMDGAVQTLYPRKNWSSMVLFNCGHPKNRAALTPDEVSTRSGAHLHRFAWLDDDEVGEVPFVWNFLVGHNRVDPDDVAGTTPRAIHYTSGGPWFERYKDCEFADLWIQERDAYESEEKQAEEVNKPMAAAPVPSPAVSVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2I6</accession>
    <name>I1H2I6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PHD finger protein ALFIN-LIKE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100822053</name>
        <name evidence="15" type="ORF">BRADI_1g53660v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the Alfin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557359.1">
        <property type="nucleotide sequence ID" value="XM_003557311.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2I6"/>
    <dbReference type="FunCoup" id="I1H2I6">
        <property type="interactions" value="1903"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H2I6"/>
    <dbReference type="EnsemblPlants" id="KQK20300">
        <property type="protein sequence ID" value="KQK20300"/>
        <property type="gene ID" value="BRADI_1g53660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822053"/>
    <dbReference type="Gramene" id="KQK20300">
        <property type="protein sequence ID" value="KQK20300"/>
        <property type="gene ID" value="BRADI_1g53660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822053"/>
    <dbReference type="eggNOG" id="KOG1632">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058315_1_0_1"/>
    <dbReference type="OMA" id="YSMCDPE"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15613">
        <property type="entry name" value="PHD_AL_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000306">
        <property type="entry name" value="PHD finger alfin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045104">
        <property type="entry name" value="Alfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021998">
        <property type="entry name" value="Alfin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044104">
        <property type="entry name" value="PHD_AL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321">
        <property type="entry name" value="CPG BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321:SF39">
        <property type="entry name" value="PHD FINGER PROTEIN ALFIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12165">
        <property type="entry name" value="Alfin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="190"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="136"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU369089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK20300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="246" mass="27722" checksum="D94089861B2FE18C" modified="2012-06-13" version="1">MEMAPAPVSPTPRTVDDIYKDFSNRRTALVRALTVDVDDFYGFCDPEKENLCLYGYPNGSWEVALPAEEVPPEMPEPALGINFARDGMNRRDWLSLVAVHADSWLLAVAFFFGARLNANDRKRLFSMINDQSTVLESLSERKHGRDNKSGGDNSGKSRHSAKRANNDVQTKNPRPVAVDVAYEDDDEHSETLCGTCSGRYNASEFWIGCDICERWFHGKCVRITPAKAEHIKHYKCPDCSSKKSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2R8</accession>
    <name>I1H2R8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Survival protein SurE-like phosphatase/nucleotidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100836897</name>
        <name evidence="5" type="ORF">BRADI_1g54410v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557407.1">
        <property type="nucleotide sequence ID" value="XM_003557359.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2R8"/>
    <dbReference type="STRING" id="15368.I1H2R8"/>
    <dbReference type="EnsemblPlants" id="KQK20420">
        <property type="protein sequence ID" value="KQK20420"/>
        <property type="gene ID" value="BRADI_1g54410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836897"/>
    <dbReference type="Gramene" id="KQK20420">
        <property type="protein sequence ID" value="KQK20420"/>
        <property type="gene ID" value="BRADI_1g54410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836897"/>
    <dbReference type="eggNOG" id="ENOG502QUQI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045192_2_0_1"/>
    <dbReference type="OMA" id="IKMGWRQ"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008252">
        <property type="term" value="F:nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1210.10:FF:000003">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="5'-NUCLEOTIDASE SURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457:SF0">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G01070)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="4">
        <location>
            <begin position="10"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK20420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="298" mass="32026" checksum="42C2CEDCC565388D" modified="2012-06-13" version="1">MASTAAAPVVMVTNDDGIDAPGLRFLVDQLVAEGRFRVLVCAPDTDRSGVSHCITWRPALCCKRVNISGATAFGVSGTPADCASLGISGKLFDGLVPDLVLSGINIGNNCGFHVVYSGTVAGAREAFINDIPALAMSYDWVAGQSSVNDLKVAAEVCMPLINAVMVEIKNGTYPKGSFLNIDVPTDAAHHKGYKITKQGKYMARIGWEQTVYKKPAVESYQTANINDDSEKDSEVDTSSESDLLFKRVIVKRSYDEEDGEDMDHKSLVDGYITVTPLGALSRAEADVIPYYKACLSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H555</accession>
    <name>I1H555_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Peroxidase</fullName>
            <ecNumber evidence="3 19">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC100842678</name>
        <name evidence="21" type="ORF">BRADI_1g61530v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">Bd21</strain>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3 19"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557849.1">
        <property type="nucleotide sequence ID" value="XM_003557801.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H555"/>
    <dbReference type="STRING" id="15368.I1H555"/>
    <dbReference type="EnsemblPlants" id="KQK21558">
        <property type="protein sequence ID" value="KQK21558"/>
        <property type="gene ID" value="BRADI_1g61530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842678"/>
    <dbReference type="Gramene" id="KQK21558">
        <property type="protein sequence ID" value="KQK21558"/>
        <property type="gene ID" value="BRADI_1g61530v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842678"/>
    <dbReference type="eggNOG" id="ENOG502QPX7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010543_0_1_1"/>
    <dbReference type="OMA" id="NCRAVNH"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000028">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 19" id="KW-0349">Heme</keyword>
    <keyword evidence="13 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="9 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="6 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982698" description="Peroxidase" evidence="19">
        <location>
            <begin position="23"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="35"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="45"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="78"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="132"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="215"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQK21558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="338" mass="35854" checksum="88ECCB26C5B51912" modified="2012-06-13" version="1">MGKVAATLVVPALLMALAFCQAAGYYEPPSPGKCGLTVGYYHESCPHAEDIVKGVVAEAVRRNPGIGAGLIRMLFHDCFVEGCDASVLLDPTPANPQPEKLSPPNFPSLRGFEVIDAAKDILEKACPGVVSCADIVAFAARDASFFLSKRGRGVAFQMPAGRLDGRVSIASRALDLLPPPTFGLAQLVDNFAAKGLSAEDMVVLSGSHTVGRSHCSSFVPDRLAVPSDIDPALGTSLRGQCPASPSPADDPTVVQDVVTPGKLDNQYYKNVLAHKVLFTSDAALLASAETAKMVLDNANIPGWWEDRFEVAMVKMASIEVKTGRGDGEVRRNCRVVNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1H715</accession>
    <name>I1H715_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydroxyproline-rich glycoprotein family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100835456</name>
        <name evidence="2" type="ORF">BRADI_1g66850v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558251.1">
        <property type="nucleotide sequence ID" value="XM_003558203.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H715"/>
    <dbReference type="STRING" id="15368.I1H715"/>
    <dbReference type="EnsemblPlants" id="KQK22367">
        <property type="protein sequence ID" value="KQK22367"/>
        <property type="gene ID" value="BRADI_1g66850v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK22371">
        <property type="protein sequence ID" value="KQK22371"/>
        <property type="gene ID" value="BRADI_1g66850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835456"/>
    <dbReference type="Gramene" id="KQK22367">
        <property type="protein sequence ID" value="KQK22367"/>
        <property type="gene ID" value="BRADI_1g66850v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22371">
        <property type="protein sequence ID" value="KQK22371"/>
        <property type="gene ID" value="BRADI_1g66850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835456"/>
    <dbReference type="eggNOG" id="ENOG502QWA4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="AYISLAH"/>
    <dbReference type="OrthoDB" id="1927968at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H715">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040420">
        <property type="entry name" value="At1g76660-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31798">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31798:SF3">
        <property type="entry name" value="OS01G0103800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="56309" checksum="EC6ED94724D8E69A" modified="2012-06-13" version="1">MATPGSSTGGGGSSTRAANGAVAISAAATAAGSADARFHSQPPHQDRQSRWAGCFSGLSCFGSQKGGKRIVPAARMPDGNASTNRGNALQSGGNSNQNGALNLSLLAPPSSPASFSNSALPSTAQSPNCFLSISANSPGGPTSNMFAVGPYANEPQLVSPPTAFSTYTTEPSTAPLTPPPELAHATTPSSPDVPYARFLSSSMGLKTAGKEHNMHYLSTAYSGGSGLQGSYPLYPGSPSSSLISPASATPRTGLSSPIPEQDVPTAHWKISRSACDTPYSIASPIPEQEVPTAQWKTSRSACDTPYSRTSPSNIFGLDSAAPRNCLLDSNFFRPAASAQFYLDQAQQTFPYNGGRLSVSRDKQDADEVEAYRASFGFSADEIVTTQHYAEIPDTLDDGFSISPFGNSAPAAEVSPFNDLPNEAQKVDKSLLNAKAITSPKKSADQLSSGTPQKVLHLDIFKGTKGGHLSDDDGIAKDCHPFRKSRDEISLKPIEVRKKSPPGQACSDAEIEYRRARSLREANSVLSWRSTLARQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1H981</accession>
    <name>I1H981_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100822996</name>
        <name evidence="2" type="ORF">BRADI_1g73230v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558713.1">
        <property type="nucleotide sequence ID" value="XM_003558665.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H981"/>
    <dbReference type="STRING" id="15368.I1H981"/>
    <dbReference type="EnsemblPlants" id="KQK23412">
        <property type="protein sequence ID" value="KQK23412"/>
        <property type="gene ID" value="BRADI_1g73230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822996"/>
    <dbReference type="Gramene" id="KQK23412">
        <property type="protein sequence ID" value="KQK23412"/>
        <property type="gene ID" value="BRADI_1g73230v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822996"/>
    <dbReference type="eggNOG" id="ENOG502S3Z6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098106_6_3_1"/>
    <dbReference type="OMA" id="MDCIILP"/>
    <dbReference type="OrthoDB" id="1052757at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35296">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35296:SF3">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK23412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="123" mass="13771" checksum="8098DB3B380AF25B" modified="2012-06-13" version="1">MAGCCVFFLRWPSTSPARLGYHSLDDEATAAAAGPSSSSPVTVVVGKERREFAVEQLVLDTYPFRVLLETVPRKEERRGGKALFLDVDAILFEHILWLACQRSSVSLLHLDLREIIDFYSQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9Z2</accession>
    <name>I1H9Z2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-dependent kinase inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100841374</name>
        <name evidence="6" type="ORF">BRADI_1g75887v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CDI family. ICK/KRP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558857.1">
        <property type="nucleotide sequence ID" value="XM_003558809.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H9Z2"/>
    <dbReference type="STRING" id="15368.I1H9Z2"/>
    <dbReference type="EnsemblPlants" id="KQK23758">
        <property type="protein sequence ID" value="KQK23758"/>
        <property type="gene ID" value="BRADI_1g75887v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841374"/>
    <dbReference type="Gramene" id="KQK23758">
        <property type="protein sequence ID" value="KQK23758"/>
        <property type="gene ID" value="BRADI_1g75887v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841374"/>
    <dbReference type="eggNOG" id="ENOG502QXA1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083146_0_1_1"/>
    <dbReference type="OMA" id="GRYEWEQ"/>
    <dbReference type="OrthoDB" id="6373236at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H9Z2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045740">
        <property type="term" value="P:positive regulation of DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044275">
        <property type="entry name" value="KRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46776">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017811">
        <property type="entry name" value="CDK_inhib_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="5">
        <location>
            <begin position="162"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="30"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR017811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK23758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="210" mass="23010" checksum="08EB9622EF255119" modified="2012-06-13" version="1">MGKYMRKGKVSGEVAVMEVSGALLGVRTRSRTLALQRTDGGKGEEEPAGTGDYLELRSRRLVKPPPPPTKDKEASAARWAAAAAAARRKEATAAEGFEAAADLEASFGDNVLDWDAAVERSTRETTPCSLIKSSDTISTPGSATRTSHPMSSRRRMESPVCRYIPSSLEMDEFFAAAEQQQHQIFRDKYNFCPANDCPLSGRYEWARLDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1HF09</accession>
    <name>I1HF09_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100842231</name>
        <name evidence="15" type="ORF">BRADI_2g12090v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasmic vesicle</location>
            <location evidence="9">COPII-coated vesicle membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567620.1">
        <property type="nucleotide sequence ID" value="XM_003567572.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HF09"/>
    <dbReference type="STRING" id="15368.I1HF09"/>
    <dbReference type="EnsemblPlants" id="KQK04172">
        <property type="protein sequence ID" value="KQK04172"/>
        <property type="gene ID" value="BRADI_2g12090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842231"/>
    <dbReference type="Gramene" id="KQK04172">
        <property type="protein sequence ID" value="KQK04172"/>
        <property type="gene ID" value="BRADI_2g12090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842231"/>
    <dbReference type="eggNOG" id="KOG1986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013303_0_0_1"/>
    <dbReference type="OMA" id="WMERLGH"/>
    <dbReference type="OrthoDB" id="3979788at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HF09">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048658">
        <property type="term" value="P:anther wall tapetum development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010584">
        <property type="term" value="P:pollen exine formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd11280">
        <property type="entry name" value="gelsolin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01468">
        <property type="entry name" value="trunk_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000007">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000013">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000057">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF6">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0813">Transport</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="11">
        <location>
            <begin position="172"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="12">
        <location>
            <begin position="254"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="14">
        <location>
            <begin position="497"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="13">
        <location>
            <begin position="603"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK04172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="851" mass="92209" checksum="02E026BAA80CF93C" modified="2012-06-13" version="1">MEETPPQPQPQAAHASPPFPAVFTPPSGLSSASVRGSPSIPSPGGTAHFSTPPGPPVFSSPLRPAAVPFRATPVSLHPVPFAIRTASSSSSVSLPTSSAPHFLNGSATHHGHLPSAASHLEDSSVDSPYVLFSAHKVLKQKKLLNAPSLGFGALVSPGREVAPGPEALERDPRRCLNCGAYVNLYCDVLIGSGQWQCVICKKLNSSEGEFVVSSKQDLLQWPELATSAVDYVHSGNRRPGFVPVSDSRVSGPIFILIDECLDEAHLQHLQGSLHAFVDSLPPTARIGIISYGRTVSVYDFSEGVAVSADVLPGNKSPTHESLKALIYGTGVYFSPIHASLPVAHTIFSSLRPYELSVAEVSRDRCVGAAVEVALGIIQGPSVELSRGIIKRSGGNCRILVCAGGPNTFGPGSVPHSVQHPNYAYLEKTAMKWMESLGHEAQRHSTIVDILCAGTCPVRVPVLQPLAKCSGGVLLLHDDFGEAFGVNLQRASTRAAGSHGLFEIRCSDGILVTQVIGPGEEASPDSHETFKHDTSFCIQMHSVEETQSFSVSMETKGDINSDFVYFQFAVRYSNKYQAEITRVITSRLQTVDGLSAYLSTVQGDVASVIIGKRTVLRARTASDAIDMRLTIDERVKDIALKFGTQVPKSKLYRFPKELASLPESLFHLRRGPLLGSIVGHEDERSVLRNLFLNASFDLSLRMLAPRCIMHREGGTFEELPAYDLAMQSYSAVVLDHGTDIFIWLGAELATKEGQSAAALAACRTLAEELSELRFPAPRILSFREGSSQARYFVSRLIPAHKDPTYEQESRFPQLRTLTPELRAKLKSSFIHFDDHSFCEWMRSLKLVPPEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HH73</accession>
    <name>I1HH73_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Choline kinase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100834192</name>
        <name evidence="2" type="ORF">BRADI_2g18690v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568021.1">
        <property type="nucleotide sequence ID" value="XM_003567973.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HH73"/>
    <dbReference type="FunCoup" id="I1HH73">
        <property type="interactions" value="811"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HH73"/>
    <dbReference type="EnsemblPlants" id="KQK05205">
        <property type="protein sequence ID" value="KQK05205"/>
        <property type="gene ID" value="BRADI_2g18690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834192"/>
    <dbReference type="Gramene" id="KQK05205">
        <property type="protein sequence ID" value="KQK05205"/>
        <property type="gene ID" value="BRADI_2g18690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834192"/>
    <dbReference type="eggNOG" id="KOG2686">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012712_0_2_1"/>
    <dbReference type="OMA" id="NYICEYM"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004103">
        <property type="term" value="F:choline kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF93">
        <property type="entry name" value="RE24176P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK05205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="41416" checksum="3D0BA9DA44D30575" modified="2012-06-13" version="1">MVAIEGQSPGEAASAAAAAAAAAGRSIPKEAARLLHELAAEWADVADCRALEVAPLKGAMTNEVYQVRWLAAGAGAGAAGEEGGEYRKVLLRVYGEGVEVFFDREDEVRTFECMSRHGHGPRLLGRFPTGRVEEFIHARTLSAVDLRDPEISSIIASKLREFHNLDMPGPKSTLLWDRLRNWLKTAKSVCPSDEAKEFCLDSMEEEITALENLFSGEDHRIGFCHNDLQYGNIMIDEETKALTIIDYEYASFNPVAYDIANHFCEMAADYHSEKPHILDYTKYPDIDEQKRFVQTYLNSSGEEPDAEKVNDLMKCVEKHTLASHLIWGLWGIISEHVNDIDFDYMEYARQRFTQFWLKEPAILGGCEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1HIX0</accession>
    <name>I1HIX0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100826542</name>
        <name evidence="2" type="ORF">BRADI_2g23750v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568341.1">
        <property type="nucleotide sequence ID" value="XM_003568293.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HIX0"/>
    <dbReference type="FunCoup" id="I1HIX0">
        <property type="interactions" value="1174"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK05985">
        <property type="protein sequence ID" value="KQK05985"/>
        <property type="gene ID" value="BRADI_2g23750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826542"/>
    <dbReference type="Gramene" id="KQK05985">
        <property type="protein sequence ID" value="KQK05985"/>
        <property type="gene ID" value="BRADI_2g23750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826542"/>
    <dbReference type="eggNOG" id="ENOG502QQB3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021229_1_0_1"/>
    <dbReference type="OMA" id="DHDTMKQ"/>
    <dbReference type="OrthoDB" id="1924189at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312:SF190">
        <property type="entry name" value="OS05G0447700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK05985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="481" mass="51728" checksum="0A7AE47B6BC1566F" modified="2012-06-13" version="1">MGFQVAAVVPSPRAPSTASSSPSTSSRTPSLSGAGCARLARSRAPFSLGGGRRRGGEVRRALSASLDGMGGGGETEFLRRIEELAASAGVRTWPPNVERSASSVALPVSLRMLKRKKQQRQVAPPPRPQSMWDERLLGSSAGESVGRAFSSMVLIVRELQSFALQQMRESLLCGDDLQGVLARAHGEMHASFVWLFQHIFAGTPALMLSLMLLLANFTVHSMGHSVAAAAAVPPTPPTASAAATLVDYTEASNPRFDAASVRTFSVGRTASVDGGSGGGGKVRPVAGAQGDDRWDDSHHSLSRVAPQQPAPLAGTGAEKILPEDEQAIWERMVAEASRMQANAELTDPDLLGNLVAPVEAEMETEDLAEFARTEQRYELAVSEEPYNSLVLANFAQFLYLTRKDHRRAEHYFERAVQAEPTDAEARSRYATFLWKARDDVEAAEENYLEAIAADPGNAHYAAAYANFLWNTGGDETCYPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HKP8</accession>
    <name>I1HKP8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100834311</name>
        <name evidence="22" type="ORF">BRADI_2g31540v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">Bd21</strain>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 25">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 25">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568703.1">
        <property type="nucleotide sequence ID" value="XM_003568655.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HKP8"/>
    <dbReference type="EnsemblPlants" id="KQK06928">
        <property type="protein sequence ID" value="KQK06928"/>
        <property type="gene ID" value="BRADI_2g31540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834311"/>
    <dbReference type="Gramene" id="KQK06928">
        <property type="protein sequence ID" value="KQK06928"/>
        <property type="gene ID" value="BRADI_2g31540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834311"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_42_1_1"/>
    <dbReference type="OMA" id="TMGPDPK"/>
    <dbReference type="OrthoDB" id="1903759at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000003">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000007">
        <property type="entry name" value="FERONIA receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000058">
        <property type="entry name" value="Receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590">
        <property type="entry name" value="OS03G0124300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34590:SF5">
        <property type="entry name" value="OS04G0586500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0278">Fertilization</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094694" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="26"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="528"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="809"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="821"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KQK06928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="871" mass="95131" checksum="41F864AF888D4A4E" modified="2012-06-13" version="1">MRARQHLFLAELLLVISWVLLFCDAEKYEPTETILVDCGSEKDGQDAQGRKWVMDKDSKWLGDGGKSSMMAAADAQDPSLPSPVPYMSARVFTKEAVYTFPVADADRHWVRLHFYPAAYHDLPAEQFFFSVSTASGITLLRNFSVYITVKALSQAYIVREFTLPPSTTGSISLKFTPTAMNNASYAFVNGIEIISMPNIFAEPATLVGLDSQTVDLAAGSLQTMYRLNVGGAYVASTNDSGLSREWFDDTPYIYGAATGVTFEPNDTFPIKYPSPEGEFAAPADVYITSRSMGPDGRVNKNNNLTWVFEVDANFTYVLRLHFCGLRVDKVNQVVFDIYINNKTAQDNADIIGWSSAKDVPVFKDYAVFMPDMPGDKILWLALHPDVDSKPQFFDAILNGLEIFKMSDGSGNLAGPNPDPSKMLMESEVEQGKFRAKPSNLQATLIGGAAGGAAALGIVAAICLVVYQTKKNRALSSSPSHSSGWLPVYGGNSHTNASSGSRSAALNPNITAMCRHFSFPEIKSATKNFDEGLVIGVGGFGKVYKGVVDGDTKVAIKRSNPSSEQGVMEFQTEIEMLSKLRHKHLVSLIGCCEDDGEMILVYDYMAHGTLREHLYKSGKPPLLWKQRLEIVIGAARGLHYLHTGAKYTIIHRDVKTTNILVDEKWVAKVSDFGLSKTGPTAQNQSHVSTMVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVLFEVLCARPALNPSLPREQVSLADHALSCQRKGTLQDIVDPLLKGKIAPDCMKKFAETAEKCLADHGVDRPSMGDVLWNLEFALQMQETFENGGKPEGGDSVGSSSSGSTPPSMADSMAANAAALSLICEDMDEEDIANSVVLSQLVRPTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQU1</accession>
    <name>I1HQU1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">MYB transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BRADI_2g47820v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Binds preferentially double-stranded telomeric repeats, but may also bind to the single telomeric strand.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Forms a homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQU1"/>
    <dbReference type="FunCoup" id="I1HQU1">
        <property type="interactions" value="846"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HQU1"/>
    <dbReference type="EnsemblPlants" id="KQK09410">
        <property type="protein sequence ID" value="KQK09410"/>
        <property type="gene ID" value="BRADI_2g47820v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09410">
        <property type="protein sequence ID" value="KQK09410"/>
        <property type="gene ID" value="BRADI_2g47820v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839702"/>
    <dbReference type="eggNOG" id="ENOG502QTN3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047477_1_0_1"/>
    <dbReference type="OMA" id="QCCRDEV"/>
    <dbReference type="OrthoDB" id="608866at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HQU1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000620">
        <property type="entry name" value="Homeodomain-like/winged-helix DNA-binding family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000168">
        <property type="entry name" value="Telomere repeat-binding factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044597">
        <property type="entry name" value="SMH1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46267">
        <property type="entry name" value="SINGLE MYB HISTONE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46267:SF15">
        <property type="entry name" value="WINGED HELIX-TURN-HELIX TRANSCRIPTION REPRESSOR DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0779">Telomere</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="14">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="13">
        <location>
            <begin position="5"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="H15" evidence="15">
        <location>
            <begin position="115"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK09410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="33248" checksum="BAACD60A4107278F" modified="2012-06-13" version="1">MGAPKQKWTSEEEEALRRGVVKHGAGKWRTIQKDPEFSPVLSSRSNIDLKDKWRNLSFSANGLGSRDKIRVPRIKGPSSSSSPSPQLLLLPAPYNVAEASPAEDPEKSPQDDKTPSPKYSAMILEALAELNEPIGSEITTIYHYIEQRHEVQPNFRRLLCAKLRRLIGAKKVEKIDKAYKLTDSYAKKALAPMKDSSLKKKDPAKPSKASKNLSPGVEAAGAAAMKLVDAEAKAHLAKDHMVEAARISRMAEDTESLLMLATELYERCSRGEMITMLQVAAQREDQTHQSHANIRSFVHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HRY0</accession>
    <name>I1HRY0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YqgF/RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100833580</name>
        <name evidence="6" type="ORF">BRADI_2g50830v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569889.1">
        <property type="nucleotide sequence ID" value="XM_003569841.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315215.1">
        <property type="nucleotide sequence ID" value="XM_024459447.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315216.1">
        <property type="nucleotide sequence ID" value="XM_024459448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HRY0"/>
    <dbReference type="STRING" id="15368.I1HRY0"/>
    <dbReference type="EnsemblPlants" id="KQK09895">
        <property type="protein sequence ID" value="KQK09895"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09897">
        <property type="protein sequence ID" value="KQK09897"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09898">
        <property type="protein sequence ID" value="KQK09898"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09899">
        <property type="protein sequence ID" value="KQK09899"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09900">
        <property type="protein sequence ID" value="KQK09900"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09901">
        <property type="protein sequence ID" value="KQK09901"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09902">
        <property type="protein sequence ID" value="KQK09902"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72909">
        <property type="protein sequence ID" value="PNT72909"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72911">
        <property type="protein sequence ID" value="PNT72911"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833580"/>
    <dbReference type="Gramene" id="KQK09895">
        <property type="protein sequence ID" value="KQK09895"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09897">
        <property type="protein sequence ID" value="KQK09897"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09898">
        <property type="protein sequence ID" value="KQK09898"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09899">
        <property type="protein sequence ID" value="KQK09899"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09900">
        <property type="protein sequence ID" value="KQK09900"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09901">
        <property type="protein sequence ID" value="KQK09901"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09902">
        <property type="protein sequence ID" value="KQK09902"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72909">
        <property type="protein sequence ID" value="PNT72909"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72911">
        <property type="protein sequence ID" value="PNT72911"/>
        <property type="gene ID" value="BRADI_2g50830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833580"/>
    <dbReference type="eggNOG" id="ENOG502RXZ7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098240_1_0_1"/>
    <dbReference type="OMA" id="YTYWDEC"/>
    <dbReference type="OrthoDB" id="10261669at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HRY0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000967">
        <property type="term" value="P:rRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16964">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.140:FF:000008">
        <property type="entry name" value="Putative pre-16S rRNA nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00651">
        <property type="entry name" value="Nuclease_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005227">
        <property type="entry name" value="YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00250">
        <property type="entry name" value="RNAse_H_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317:SF1">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03652">
        <property type="entry name" value="RuvX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="YqgF/RNase H-like" evidence="5">
        <location>
            <begin position="23"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK09895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="175" mass="19490" checksum="BF1128B5BBAF894F" modified="2012-06-13" version="1">MRLIKAEELFRKVLESGAKKQKNRLLGLDVGSKYVGLAVSDHQNRIALPLSVLGRTKTNITLMADDFKTLVKKYSLAGLVVGYPFNLQGQCSPDAIQVSLLVGELCKTGKLDDLSYTYWDENFTSKCVEALLNPLKLHDPVETKTMTDKFAAVCILQGYLDNMNRALRSTDNCKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1HVT5</accession>
    <name>I1HVT5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100844769</name>
        <name evidence="10" type="ORF">BRADI_2g62510v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232888.1">
        <property type="nucleotide sequence ID" value="XM_010234586.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HVT5"/>
    <dbReference type="STRING" id="15368.I1HVT5"/>
    <dbReference type="EnsemblPlants" id="KQK11802">
        <property type="protein sequence ID" value="KQK11802"/>
        <property type="gene ID" value="BRADI_2g62510v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK11805">
        <property type="protein sequence ID" value="KQK11805"/>
        <property type="gene ID" value="BRADI_2g62510v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844769"/>
    <dbReference type="Gramene" id="KQK11802">
        <property type="protein sequence ID" value="KQK11802"/>
        <property type="gene ID" value="BRADI_2g62510v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11805">
        <property type="protein sequence ID" value="KQK11805"/>
        <property type="gene ID" value="BRADI_2g62510v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844769"/>
    <dbReference type="eggNOG" id="KOG0048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028567_25_0_1"/>
    <dbReference type="OMA" id="WMEQANI"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HVT5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000011">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000517">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044676">
        <property type="entry name" value="EOBI/EOBII-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675:SF7">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="35"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="36"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="85"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="85"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="137"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="153"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK11802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="264" mass="30136" checksum="965F0818FDD0A65A" modified="2012-06-13" version="1">MKQTSKQQLGKTKKMAIEAGKKKKEECLSAAAGVVRKGPWTEQEDMKLVWFVRLFGERRWDFLAKVSGLNRSGKSCRLRWVNYLHPDLKRGRMSPDEERLVVELHAKWGNRWSRIARSMPGRTDNEIKNYWRTHTRKLKHKHRHHQEEEESTKVATSASASTSTSAATTSPSSTSSSSDVEVVQEAASKEDEQQQLLCAGTMDDGQLLWNDDGIDAYASWSGATSSMMMVPSSPVWPWDDHYCCPTDSLWGHDEVFEYKKMLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZX2</accession>
    <name>I1HZX2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100830840</name>
        <name evidence="4" type="ORF">BRADI_3g11390v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573193.1">
        <property type="nucleotide sequence ID" value="XM_003573145.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZX2"/>
    <dbReference type="STRING" id="15368.I1HZX2"/>
    <dbReference type="EnsemblPlants" id="KQJ94592">
        <property type="protein sequence ID" value="KQJ94592"/>
        <property type="gene ID" value="BRADI_3g11390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830840"/>
    <dbReference type="Gramene" id="KQJ94592">
        <property type="protein sequence ID" value="KQJ94592"/>
        <property type="gene ID" value="BRADI_3g11390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830840"/>
    <dbReference type="eggNOG" id="KOG0714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_10_2_1"/>
    <dbReference type="OMA" id="TECTVNI"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000002">
        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000020">
        <property type="entry name" value="DnaJ subfamily B member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000006">
        <property type="entry name" value="DnaJ subfamily B member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF529">
        <property type="entry name" value="HEAT-SHOCK PROTEIN DNAJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ94592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="349" mass="38559" checksum="AEAAA40A00B464E5" modified="2012-06-13" version="1">MGLDYYKVLQVERGASDDELKKAYRKLAMKWHPDKNPNNKKESEAKFKQISEAYEVLSDSQKRAVYDQYGEEALKGQVPPPGAGGPSGSSYYGGDGSTFRFNPRSADDIFAEFFGFSSPFSSTGGMGGMGGGAERGMRGSRFGMFGDDMFGSFPQFHGEASMHAPQRSHKAGPIENRLPCNLADLYKGTTKKMKISREILDSSGRTMVVEEILTIEIKPGWKKGTKITFPEKGNESPHVIPADIVFVIDEKPHDQFTRDGNDLVMTQKISLAEALTGCTVHVTTLDGRNLPVPINTVVNPGYEEVIPREGMPIPKDPSKKGNLKIKFNIKFPSRLMPEQKLEIKRLLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2Q2</accession>
    <name>I1I2Q2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Sucrose-phosphate synthase</fullName>
            <ecNumber evidence="4 9">2.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100825323</name>
        <name evidence="14" type="ORF">BRADI_3g20120v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Plays a role in photosynthetic sucrose synthesis by catalyzing the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose- 6-phosphate. Involved in the regulation of carbon partitioning in the leaves of plants. May regulate the synthesis of sucrose and therefore play a major role as a limiting factor in the export of photoassimilates out of the leaf. Plays a role for sucrose availability that is essential for plant growth and fiber elongation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>beta-D-fructose 6-phosphate + UDP-alpha-D-glucose = sucrose 6(F)-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:57723"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Glycan biosynthesis; sucrose biosynthesis; sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyltransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.14" evidence="4 9"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573681.1">
        <property type="nucleotide sequence ID" value="XM_003573633.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2Q2"/>
    <dbReference type="STRING" id="15368.I1I2Q2"/>
    <dbReference type="EnsemblPlants" id="KQJ95996">
        <property type="protein sequence ID" value="KQJ95996"/>
        <property type="gene ID" value="BRADI_3g20120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825323"/>
    <dbReference type="Gramene" id="KQJ95996">
        <property type="protein sequence ID" value="KQJ95996"/>
        <property type="gene ID" value="BRADI_3g20120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825323"/>
    <dbReference type="eggNOG" id="KOG0853">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_24_0_1"/>
    <dbReference type="OMA" id="AWSKGIS"/>
    <dbReference type="OrthoDB" id="512920at2759"/>
    <dbReference type="UniPathway" id="UPA00371">
        <property type="reaction ID" value="UER00545"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046524">
        <property type="term" value="F:sucrose-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16419">
        <property type="entry name" value="HAD_SPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006380">
        <property type="entry name" value="SPP-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044161">
        <property type="entry name" value="SPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035659">
        <property type="entry name" value="SPS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012819">
        <property type="entry name" value="SPS_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000368">
        <property type="entry name" value="Sucrose_synth_GT-B1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02468">
        <property type="entry name" value="sucrsPsyn_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039:SF2">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00862">
        <property type="entry name" value="GT-B_Sucrose_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05116">
        <property type="entry name" value="S6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sucrose synthase first GT-B" evidence="12">
        <location>
            <begin position="190"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="11">
        <location>
            <begin position="493"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose phosphatase-like" evidence="13">
        <location>
            <begin position="808"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="692"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ95996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1064" mass="118201" checksum="0EA6D154063E3DC9" modified="2012-06-13" version="1">MVGNDWINSYLEAILDAGGAAGDISAASVAGGGDGPGAGGADGEKRDKSSLMLRERGRFNPARYFVEEVISGFDETDLYKTWVRTSAMRSPQERNTRLENMSWRIWNLARKKKQIEGEEASRSSKKRLEREKARRDAAADLSEDLSEGEKGENIHESSIHDESTRGRMPRIGSTDAIEVWANQHKDKKLYIVLISIHGLIRGENMELGRDSDTGGQVKYVVELARALGETPGVYRVDLLTRQISAPDVDWSYGEPTEMLSPRNSENLGDDMGESSGAYIVRIPFGPRDKYIPKEHLWPHIQEFVDGALVHIMQMSKVLGEQVGSGQPVWPVVIHGHYADAGDSAALLSGALNVPMVFTGHSLGRDKLEQLLKQGRQTRDEVDATYKIMRRIEAEELCLDASEIIITSTRQEIEKQWGLYNGFDLTMAKKLRARMKRGVSCYGRYMPRMIAIPPGMEFGHIVPHDVDLDGEEGNEIGSGSPDPPIWTDIMRFFSNPRKPMILALARPDPKKNITTLVKAFGEHHELRNLANLTLIMGNRDVIDEMSSTNGAVLTSVLKLIDKYDLYGQVAYPKHHKQSDVPDIYRLAARTKGVFINPAYIEPFGLTLIEAAAYGLPMVATQNGGPVDIHRVLDNGILVDPHNQNDIAEALYKLVSDKHLWAKCRENGLQNIHRFSWPEHCKNYLSRVGTLKPRHPRWQRSDDATEISEADSPGDSLRDLHDISLNLKISLDSEKSSTKRNLEDALLKFSKGVSASTKDESGENAEATTGSNKWPSLRRRKHIVVIAVDSVQDADLVQIIKNIFEASSKERLSGDVGFVLSTSRAVSEIHPLLTSGGIETTDFDAFICSSGSDLCYPSASSEDMLSPSELPFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKNSESGQKVVVEDEECSSTYCISFKVKNSEAVPPVKDLRKTMRIQALRCHVLYSHDGSKLNLIPVLASRSQALRYLYIRWGVELSNMTVVVGESGDTDYDGLLGGVHKTIILKGSFNAAPNQLHAARGYSLEDVVSFDKPGISSVEGYLPDDLKSALQQFGVLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5E8</accession>
    <name>I1I5E8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100840129</name>
        <name evidence="7" type="ORF">BRADI_3g30830v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234915.1">
        <property type="nucleotide sequence ID" value="XM_010236613.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316989.1">
        <property type="nucleotide sequence ID" value="XM_024461221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5E8"/>
    <dbReference type="STRING" id="15368.I1I5E8"/>
    <dbReference type="EnsemblPlants" id="KQJ97462">
        <property type="protein sequence ID" value="KQJ97462"/>
        <property type="gene ID" value="BRADI_3g30830v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ97463">
        <property type="protein sequence ID" value="KQJ97463"/>
        <property type="gene ID" value="BRADI_3g30830v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67719">
        <property type="protein sequence ID" value="PNT67719"/>
        <property type="gene ID" value="BRADI_3g30830v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67720">
        <property type="protein sequence ID" value="PNT67720"/>
        <property type="gene ID" value="BRADI_3g30830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840129"/>
    <dbReference type="Gramene" id="KQJ97462">
        <property type="protein sequence ID" value="KQJ97462"/>
        <property type="gene ID" value="BRADI_3g30830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97463">
        <property type="protein sequence ID" value="KQJ97463"/>
        <property type="gene ID" value="BRADI_3g30830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67719">
        <property type="protein sequence ID" value="PNT67719"/>
        <property type="gene ID" value="BRADI_3g30830v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67720">
        <property type="protein sequence ID" value="PNT67720"/>
        <property type="gene ID" value="BRADI_3g30830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840129"/>
    <dbReference type="eggNOG" id="KOG1116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015136_1_0_1"/>
    <dbReference type="OMA" id="DHPEVKP"/>
    <dbReference type="OrthoDB" id="3853857at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001729">
        <property type="term" value="F:ceramide kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF111">
        <property type="entry name" value="CERAMIDE KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="6">
        <location>
            <begin position="224"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="471"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="544"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT67720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="748" mass="82725" checksum="D17900DC1163096C" modified="2012-06-13" version="1">MQKSDNHEQKLTSPRGLIHKVFRRTNSRRSPTAAEQDPSPVFPETSNAKFLKQNNSEDAIKDQEKASTHDNRIEDEKSDLLGYEVCSGKLTLDYKGRSVSAEQSSGNCYDARLTTEAFVWGSNILKLEDIVSVSYNSGLRHFTVHACPLEKRSIGLSCFMKPRRSQKDLRFLSAAPHEAFQWVNGFADQQCYVNLLPHPMASSKKHSSELIPFDAMLDPYVKCRSPPKILVILNPRSGHGRSSKVFHGKVEPIFKLAGFKMEVVKTTHAGHAKSLVSTIDFSTCPDGIVCVGGDGIVNEVLNGLLCRDDQNESVSIPIGIIPAGSDNSLVWTVLGVKDPISAALSIVRGGFTPIDVFSVEWIQSGTTHFGTTVSYFGFVSDVLELSEKYQKRFGPLRYIVAGFLKFLCLPKYSFELEYLPVSNADGPEHKTVEGQEKVDTSDLYDDVVQRSRAECLPRASSLSSIDSVMSTSIMSGGEPEVSSPHANNEPSELVRALDPKSKRLSLGRTSTFKEPEEVLHPQASTPSWCRSKSKSRTEKAWPGLTTTNDTKSSRGTTTHDKEDTSSTVSDPGPAWDTGPKWDMEPKWDNQHNWESETPIELHGPSDDIELGLTKELVPSLDERWVVRKGRYLGVLVCNHSCKTVQSLSSQVVAPKAEYDDNCLDLLLVGGSGRLRLLRFLVLLQFGKHISLPHVEYLKVKSVRLKAGPNTHDGCGIDGELLHVKGQVRCSLLPQQCRLIGRPAQNPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6U3</accession>
    <name>I1I6U3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100830537</name>
        <name evidence="3" type="ORF">BRADI_3g35250v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574446.1">
        <property type="nucleotide sequence ID" value="XM_003574398.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6U3"/>
    <dbReference type="FunCoup" id="I1I6U3">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ98167">
        <property type="protein sequence ID" value="KQJ98167"/>
        <property type="gene ID" value="BRADI_3g35250v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ98168">
        <property type="protein sequence ID" value="KQJ98168"/>
        <property type="gene ID" value="BRADI_3g35250v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ98169">
        <property type="protein sequence ID" value="KQJ98169"/>
        <property type="gene ID" value="BRADI_3g35250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830537"/>
    <dbReference type="Gramene" id="KQJ98167">
        <property type="protein sequence ID" value="KQJ98167"/>
        <property type="gene ID" value="BRADI_3g35250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ98168">
        <property type="protein sequence ID" value="KQJ98168"/>
        <property type="gene ID" value="BRADI_3g35250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ98169">
        <property type="protein sequence ID" value="KQJ98169"/>
        <property type="gene ID" value="BRADI_3g35250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830537"/>
    <dbReference type="eggNOG" id="KOG1603">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100095_1_0_1"/>
    <dbReference type="OMA" id="QFYTVEL"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF103">
        <property type="entry name" value="OS08G0403300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="30"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ98167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="152" mass="16968" checksum="D84503F9C0811630" modified="2012-06-13" version="1">MGGTLRFLSDVLLGLGGGTGEGRHKKRRQFYTVELKVRMDCDGCERKVRNALATMRGVQTVEINRKQQKVTVQGFVEPQRVLRRALSTGKRAELWPYVPYTNPYMAPPVYDKRAPAGHVRKTDAAVMPASAAQEERLATLFSDDNPNACSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6Y7</accession>
    <name>I1I6Y7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100837592</name>
        <name evidence="8" type="ORF">BRADI_3g35620v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Phi family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574469.1">
        <property type="nucleotide sequence ID" value="XM_003574421.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6Y7"/>
    <dbReference type="FunCoup" id="I1I6Y7">
        <property type="interactions" value="786"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I6Y7"/>
    <dbReference type="EnsemblPlants" id="KQJ98231">
        <property type="protein sequence ID" value="KQJ98231"/>
        <property type="gene ID" value="BRADI_3g35620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837592"/>
    <dbReference type="Gramene" id="KQJ98231">
        <property type="protein sequence ID" value="KQJ98231"/>
        <property type="gene ID" value="BRADI_3g35620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837592"/>
    <dbReference type="eggNOG" id="KOG0867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_5_1_1"/>
    <dbReference type="OMA" id="FSIAEHK"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009635">
        <property type="term" value="P:response to herbicide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03187">
        <property type="entry name" value="GST_C_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03053">
        <property type="entry name" value="GST_N_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000004">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034347">
        <property type="entry name" value="GST_Phi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF6">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01154">
        <property type="entry name" value="Main.5:_Phi-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="87"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ98231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="214" mass="24277" checksum="0C41A6070A6EF850" modified="2012-06-13" version="1">MAMKVYGLPMSTNVARVLVCLEEVGAQYEVVPINFSIAEHKSMEHTARNPFGQVPSFQDGDLILFESRAISKYILRKNQSELVKEGELSDSAMVDMWLDVESHQFDRPMAVIIHQCLIVPMYLGGETDDNVVEENVEKLKKTFEVYEARLSMFKYLAGDFISLADLSHFPTAYYLLATPHASLLDNYPHVKVWIDDILARPTVKKVVEMMKATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1I7A5</accession>
    <name>I1I7A5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adaptor protein ClpS core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100827598</name>
        <name evidence="2" type="ORF">BRADI_3g36600v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574520.1">
        <property type="nucleotide sequence ID" value="XM_003574472.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I7A5"/>
    <dbReference type="STRING" id="15368.I1I7A5"/>
    <dbReference type="EnsemblPlants" id="KQJ98387">
        <property type="protein sequence ID" value="KQJ98387"/>
        <property type="gene ID" value="BRADI_3g36600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827598"/>
    <dbReference type="Gramene" id="KQJ98387">
        <property type="protein sequence ID" value="KQJ98387"/>
        <property type="gene ID" value="BRADI_3g36600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827598"/>
    <dbReference type="eggNOG" id="ENOG502RZXT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134083_1_0_1"/>
    <dbReference type="OMA" id="EAHHNGM"/>
    <dbReference type="OrthoDB" id="2013930at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I7A5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000006">
        <property type="entry name" value="ATP-dependent Clp protease adapter protein CLPS1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022935">
        <property type="entry name" value="ClpS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003769">
        <property type="entry name" value="ClpS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33473">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ADAPTER PROTEIN CLPS1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33473:SF17">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ADAPTER PROTEIN CLPS1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02617">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adaptor protein ClpS core" evidence="1">
        <location>
            <begin position="77"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ98387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="157" mass="16872" checksum="789D3A9D8AE80D31" modified="2012-06-13" version="1">MEAAVPSRIALSARIMNHHVVAAGGRSSIYRGRYPNIAIPIVVTAAGKGGGVLDRPAEKISPGRQSEFDVKKSRKMSPPYRVVLHNDNENRREYVVQVLMKVIPGMTVDNAVNIMQEAHVNGLAVVIVCSQSEAEEHCTSLRGNGLRSSIEPASGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IAI7</accession>
    <name>I1IAI7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proline-rich family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100841662</name>
        <name evidence="2" type="ORF">BRADI_3g45840v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575145.1">
        <property type="nucleotide sequence ID" value="XM_003575097.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IAI7"/>
    <dbReference type="FunCoup" id="I1IAI7">
        <property type="interactions" value="2619"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IAI7"/>
    <dbReference type="EnsemblPlants" id="KQJ99889">
        <property type="protein sequence ID" value="KQJ99889"/>
        <property type="gene ID" value="BRADI_3g45840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841662"/>
    <dbReference type="Gramene" id="KQJ99889">
        <property type="protein sequence ID" value="KQJ99889"/>
        <property type="gene ID" value="BRADI_3g45840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841662"/>
    <dbReference type="eggNOG" id="KOG1075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072185_0_0_1"/>
    <dbReference type="OMA" id="FSTYQKD"/>
    <dbReference type="OrthoDB" id="1931594at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061635">
        <property type="term" value="P:regulation of protein complex stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038936">
        <property type="entry name" value="MPH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35753">
        <property type="entry name" value="PROTEIN MAINTENANCE OF PSII UNDER HIGH LIGHT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35753:SF2">
        <property type="entry name" value="PROTEIN MAINTENANCE OF PSII UNDER HIGH LIGHT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ99889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="23747" checksum="6B203A771F4234F4" modified="2012-06-13" version="1">MACPAQSMLSASSCVFLRSSKPQQATMLRGGGTGIGSGRLFLCNASSSPTPSQDDSECNEVECAPEKEVGSLSVEWLAEERTKVVGTFPPKKKGWTGYVEKDTAGQTNIYSVEPAVYVAESAISSGTAGTSSEGSENTAAIAAGLALIFIAGVSSVLLQVGKNQPPPQVTAYSGPPLSYYVAKFQPAAAAFSAQPSPAVVAAAPEETPSTSDSPTVEASAADQSSPDAEQLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBR3</accession>
    <name>I1IBR3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Adenosine kinase</fullName>
            <shortName evidence="15">AK</shortName>
            <ecNumber evidence="4 15">2.7.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Adenosine 5'-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100838929</name>
        <name evidence="17" type="ORF">BRADI_3g49230v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Essential to sustain methyl recycling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>adenosine + ATP = AMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:20825"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.20" evidence="4 15"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575395.1">
        <property type="nucleotide sequence ID" value="XM_003575347.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBR3"/>
    <dbReference type="FunCoup" id="I1IBR3">
        <property type="interactions" value="2530"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IBR3"/>
    <dbReference type="EnsemblPlants" id="KQK00411">
        <property type="protein sequence ID" value="KQK00411"/>
        <property type="gene ID" value="BRADI_3g49230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838929"/>
    <dbReference type="Gramene" id="KQK00411">
        <property type="protein sequence ID" value="KQK00411"/>
        <property type="gene ID" value="BRADI_3g49230v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838929"/>
    <dbReference type="eggNOG" id="KOG2854">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045832_0_0_1"/>
    <dbReference type="OMA" id="YCATECI"/>
    <dbReference type="OrthoDB" id="432447at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00659"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006169">
        <property type="term" value="P:adenosine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000006">
        <property type="entry name" value="Adenosine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1110.10:FF:000001">
        <property type="entry name" value="Adenosine kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001805">
        <property type="entry name" value="Adenokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769:SF1">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00989">
        <property type="entry name" value="ADENOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="9 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 15" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="16">
        <location>
            <begin position="30"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK00411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="345" mass="37436" checksum="DD5B106204B5593B" modified="2012-06-13" version="1">MAASSSYDGVLLGMGNPLLDISAVVDEAFLTKYDVKLNNAILAEEKHLPMYDELSSKGNVEYIAGGATQNSIRVAQWMLQVPGATSYMGCIGKDKYGEEMKNAAQAAGVTAHYYEDEAAPTGTCAVCVVGGERSLIANLSAANCYKSEHLKKPENWALVEKAKYIYIAGFFLTVSPDSIQLVAEHAAENNKVFLMNLSAPFICEFFRDAQEKVLPYVDYIFGNETEARIFSKVRGWETENVEEIALRISQLPLASGKQKRIAVITQGADPVVVAEDGKVKTFPVILLPKEKLVDTNGAGDAFVGGFLSQLVQGKSIEDSVKAGCYAANVIIQRSGCTYPEKPDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IF41</accession>
    <name>I1IF41_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100842355</name>
        <name evidence="3" type="ORF">BRADI_3g58900v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573083.3">
        <property type="nucleotide sequence ID" value="XM_003573035.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IF41"/>
    <dbReference type="STRING" id="15368.I1IF41"/>
    <dbReference type="EnsemblPlants" id="KQK01856">
        <property type="protein sequence ID" value="KQK01856"/>
        <property type="gene ID" value="BRADI_3g58900v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01857">
        <property type="protein sequence ID" value="KQK01857"/>
        <property type="gene ID" value="BRADI_3g58900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842355"/>
    <dbReference type="Gramene" id="KQK01856">
        <property type="protein sequence ID" value="KQK01856"/>
        <property type="gene ID" value="BRADI_3g58900v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01857">
        <property type="protein sequence ID" value="KQK01857"/>
        <property type="gene ID" value="BRADI_3g58900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842355"/>
    <dbReference type="eggNOG" id="KOG1124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015299_1_0_1"/>
    <dbReference type="OMA" id="VDDCTKF"/>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044534">
        <property type="entry name" value="TTL1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46050:SF7">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46050">
        <property type="entry name" value="TPR REPEAT-CONTAINING THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="221"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="289"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK01856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="587" mass="62963" checksum="755A467C9DF56DA3" modified="2012-06-13" version="1">MTESRRAPPPPSGCNMLGIYTGMFRRRRSASMSNLSRFNNNGPVPDEPAPVSTAPPPNANHYPAHRSKPAADSSSSLVRHRPRTTPSHAPIAAPPTSSAIDKNTTIKPAAKANNAGAEYTGMAAELDKMIHDHQRVKGTTQMMRATSGNMMMHRNLGNLNASGSPSALAARASLEQQRPNPNPTPQRKNGYASTATGMGNIVGNNKAAGELCRALSHRTDPEKLKEMGNQEYREGHYAEAVALYDQAIIVDPCRPAYWSNKAAALAALGRLIEAVADCKEALRIDPSYGRAHHRLGGLYLRLGEPDKAINFFKQSSSKDHSTSADVARAQSVKSRIAKCNDARKLRDWITVLQESQAAVSDGADCAPQVLALQAEALLKLQRHDEADAALRGGGAPRFGADESAKFFGTTAHAYALTVRAQVDMAAGRFEDAVAAAQAACQLDPAGREAAAVHRRAKVVASARLRGNELFKASRFAEACAAYGEGLGNGGEATSGVLLCNRAACHAKLGRHEKAVEDCGAALALRPAYSKARLRRADCNVKLERWEAALRDYQVLIQELPENEDVKKALAEVQAKVKSQRNGGISRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IG46</accession>
    <name>I1IG46_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RMA</fullName>
            <ecNumber evidence="11">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein RING membrane-anchor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase RMA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100839731</name>
        <name evidence="14" type="ORF">BRADI_4g00760v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">E3 ubiquitin-protein ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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        <subcellularLocation>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
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        </location>
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        <location>
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        <location>
            <begin position="185"/>
            <end position="207"/>
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        <location>
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        <location>
            <begin position="113"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
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    <sequence length="233" mass="24791" checksum="37D415CD29E43C51" modified="2012-06-13" version="1">MAANVGESTSSSGGAGDGGGSFECNICFELPQEPIVTLCGHLFCWPCLYKWLHIHSHSPECPVCKAVVEEDKLVPLYGRGKDRVDPRSKNVPGAEIPHRPTGQRPATAPQADPNNHFPNANPNPWFMGGGGVPLANARWGNYTFSAAFGGLFPLLSFQVHGFPDATAYGQPAGFPYGYGHGHGHGHAFHGGHAGHPHAAPRQAQPGQQQQADVYLKALLILVGFLVIASLITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1IGF0</accession>
    <name>I1IGF0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BRADI_4g01670v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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        <property type="gene ID" value="BRADI_4g01670v3"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 4"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:polysaccharide binding"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cell surface receptor signaling pathway"/>
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        <property type="entry name" value="Os12g0614800 protein"/>
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        <property type="entry name" value="Protein kinase superfamily protein"/>
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        <property type="entry name" value="Wall-associated receptor kinase 2"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF"/>
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        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF270">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="442"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="723"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="PNT62318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="773" mass="84504" checksum="90F8413823717E6F" modified="2012-06-13" version="1">MALAMHPTFQQQAPTLILVFFQKLIVFFLAIALATPVGGTTPLALPGCPDKCGDVLIPYPFGIGAQCAAVNLNSFFNLGCNNASHPPRPTVGGPVDVAVDVVDISLEHGELRVLVPVSYICFTSSATVSATNNDTVRFSLENTPFLPSPGRNRFMVIGCNTLGLIGGFRGETSQYLAGCYSYCDGTSGASDDGAPCTGTGCCEASIPTNLTTFNVAFPINSSSVWGFNPCFYAMIAEVGWYSFQRRDLVGQLGFVNEKAKNGAPVIVNWAVRNGSCSEPRNYACVSANSFCQTAGNGPGYLCQCSPGYEGNAYLHNGCQDIDECMLHRQDPKYEELYPCRNGVCRNTPGGYDCKCKKGTKSDGTNSGCQSLHTRGLSVSAIVIISLAFFLAMRLQRKRHKEEKDEYFKQNGGLRLYDEMRSKQVDTVRILTEKEVKKATDNYNEDRVLGCGGHGMVYRGTLDDQREVAIKKSKVINDNCRDEFVNEIIILSQINHRNIVRLLGCCLDIDVPMLVYEFVSNGTLYEFLHGSADHNLSPIPLDLRLKIATQSAEALAYLHSSTSRTILHGDVKSANILLDDQYHAKVADFGASALKSIDESEFIMLVQGTLGYLDPESFISHLLTDKSDVYSFGVVLLELMTRKRALYVDNHSSEKKSLSHNFLLMFDEDRHQVMLDPEIADDAAAMAVIKNLAVLAVHCLSVRGEDRPTMTEVAERLRVLRRHQVQGAGEDKSNSYFGSNGGAPSEESEVLPLEEMTDDGNLGGLEKGKLVLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1ILR2</accession>
    <name>I1ILR2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100846345</name>
        <name evidence="5" type="ORF">BRADI_4g19047v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577565.1">
        <property type="nucleotide sequence ID" value="XM_003577517.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237722.1">
        <property type="nucleotide sequence ID" value="XM_010239420.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310734.1">
        <property type="nucleotide sequence ID" value="XM_024454966.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310735.1">
        <property type="nucleotide sequence ID" value="XM_024454967.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310736.1">
        <property type="nucleotide sequence ID" value="XM_024454968.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310737.1">
        <property type="nucleotide sequence ID" value="XM_024454969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ILR2"/>
    <dbReference type="STRING" id="15368.I1ILR2"/>
    <dbReference type="EnsemblPlants" id="KQJ88513">
        <property type="protein sequence ID" value="KQJ88513"/>
        <property type="gene ID" value="BRADI_4g19047v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ88514">
        <property type="protein sequence ID" value="KQJ88514"/>
        <property type="gene ID" value="BRADI_4g19047v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ88515">
        <property type="protein sequence ID" value="KQJ88515"/>
        <property type="gene ID" value="BRADI_4g19047v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ88516">
        <property type="protein sequence ID" value="KQJ88516"/>
        <property type="gene ID" value="BRADI_4g19047v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63637">
        <property type="protein sequence ID" value="PNT63637"/>
        <property type="gene ID" value="BRADI_4g19047v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846345"/>
    <dbReference type="Gramene" id="KQJ88513">
        <property type="protein sequence ID" value="KQJ88513"/>
        <property type="gene ID" value="BRADI_4g19047v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88514">
        <property type="protein sequence ID" value="KQJ88514"/>
        <property type="gene ID" value="BRADI_4g19047v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88515">
        <property type="protein sequence ID" value="KQJ88515"/>
        <property type="gene ID" value="BRADI_4g19047v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88516">
        <property type="protein sequence ID" value="KQJ88516"/>
        <property type="gene ID" value="BRADI_4g19047v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63637">
        <property type="protein sequence ID" value="PNT63637"/>
        <property type="gene ID" value="BRADI_4g19047v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846345"/>
    <dbReference type="eggNOG" id="KOG0055">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_1"/>
    <dbReference type="OMA" id="NKYFDQF"/>
    <dbReference type="OrthoDB" id="10255969at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060586">
        <property type="term" value="P:multicellular organismal-level iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001645">
        <property type="entry name" value="ABC transporter I family member 11 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 11, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="40"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ88516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="267" mass="29602" checksum="27DDE4E5BBD55F06" modified="2012-06-13" version="1">MFAAATVAIPPAVSPSLAKRNRGFSWSCRAERISAGYSQLEVRKVSYRPPGTEQNLLNETSLTLQEKSFGLIFGRSGSGKTTLLQLLAGLSEPTSGSICIQKYDGIGNPTGLSELLTSQRVGIVFQFPERYFLADTILEEITFGWPRQKADFLLREQLTLKLQNAIHSVGLNTISWDEDPQSLSGGFKRRLALAIQLVQTPDLLLLDEPLAGLDWKARADVVNLLKDLKKDHTILVVSHDLRELCPLVDRSWRMEMGGILKEEALPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQS3</accession>
    <name>I1IQS3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100822589</name>
        <name evidence="4" type="ORF">BRADI_4g32250v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578239.1">
        <property type="nucleotide sequence ID" value="XM_003578191.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQS3"/>
    <dbReference type="STRING" id="15368.I1IQS3"/>
    <dbReference type="EnsemblPlants" id="KQJ90526">
        <property type="protein sequence ID" value="KQJ90526"/>
        <property type="gene ID" value="BRADI_4g32250v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT64727">
        <property type="protein sequence ID" value="PNT64727"/>
        <property type="gene ID" value="BRADI_4g32250v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT64728">
        <property type="protein sequence ID" value="PNT64728"/>
        <property type="gene ID" value="BRADI_4g32250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822589"/>
    <dbReference type="Gramene" id="KQJ90526">
        <property type="protein sequence ID" value="KQJ90526"/>
        <property type="gene ID" value="BRADI_4g32250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64727">
        <property type="protein sequence ID" value="PNT64727"/>
        <property type="gene ID" value="BRADI_4g32250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64728">
        <property type="protein sequence ID" value="PNT64728"/>
        <property type="gene ID" value="BRADI_4g32250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822589"/>
    <dbReference type="eggNOG" id="KOG1196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_28_1_1"/>
    <dbReference type="OMA" id="GKHVDTY"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IQS3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd05323">
        <property type="entry name" value="ADH_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08250">
        <property type="entry name" value="Mgc45594_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF3">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="304"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT64728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="634" mass="68527" checksum="8DA71DC096C79CAD" modified="2012-06-13" version="1">MELKPGMSAFVTGGASGIGKALCLAFAQKGLFVTVVDFSEESGKEVASLVQQENKKFHGDLGVSSAIFIKCDVSNTDDLAAAFGKHVDTYGGLDICINCAGIANNTLVYDDRSDGLRTWRHAINVNLVAVIDGTRIASQIMRSLKKPGVIINIGSAAGLYPMYADPIYSGTKGGVVMFTRSLAPLKRHGVRVNVLCPEFVQTNMAEQINRKIVDATGGFMKMEEIINGAFELIKDESKAGACLWITKRRGMEYWPTSEEQRKYLLNYTKSKRTITKNAFPGIQIPEFFEKIVVHTLSHNFRNATRLDRVRLRLPIEPQNVLVKIIYAGVNASDVNYSSGRYFSGSAKETAARLPFDAGFEAVGIVASVGDAVSHIKVGSPVALMTFGSYAEFTLVPAKHLLPVPRPDPEVVAMLTSGLTASISLEKAGQMTSGQVVLVTAAAGGTGQFAVQLAKLAGNKVVATCGGESKAALLASLGVDRVINYQHEKIKDVLKKEFPRGVDIIYESVGGEMFDLCLNALAVHGRLVVIGMISQYQGEEGWKPKNYTGLCEKILAKSQTVAGFFLVQHAHMWQDHLDKLFELYASGKLKVSLDPKKFLGVASAVDAVEYLHSGKSVGKVVVCIDPSYSQTLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITW5</accession>
    <name>I1ITW5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100823011</name>
        <name evidence="10" type="ORF">BRADI_4g41100v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable anion transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Sodium/anion cotransporter (TC 2.A.1.14) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238651.1">
        <property type="nucleotide sequence ID" value="XM_010240349.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310741.1">
        <property type="nucleotide sequence ID" value="XM_024454973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITW5"/>
    <dbReference type="EnsemblPlants" id="KQJ91998">
        <property type="protein sequence ID" value="KQJ91998"/>
        <property type="gene ID" value="BRADI_4g41100v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ91999">
        <property type="protein sequence ID" value="KQJ91999"/>
        <property type="gene ID" value="BRADI_4g41100v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92000">
        <property type="protein sequence ID" value="KQJ92000"/>
        <property type="gene ID" value="BRADI_4g41100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823011"/>
    <dbReference type="Gramene" id="KQJ91998">
        <property type="protein sequence ID" value="KQJ91998"/>
        <property type="gene ID" value="BRADI_4g41100v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ91999">
        <property type="protein sequence ID" value="KQJ91999"/>
        <property type="gene ID" value="BRADI_4g41100v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92000">
        <property type="protein sequence ID" value="KQJ92000"/>
        <property type="gene ID" value="BRADI_4g41100v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823011"/>
    <dbReference type="eggNOG" id="KOG2532">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_11_1"/>
    <dbReference type="OMA" id="LITFWMP"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17380">
        <property type="entry name" value="MFS_SLC17A9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000199">
        <property type="entry name" value="Probable anion transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000220">
        <property type="entry name" value="Probable anion transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044777">
        <property type="entry name" value="SLC17A9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF282">
        <property type="entry name" value="ANION TRANSPORTER 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="367"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="12"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ91998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="436" mass="47764" checksum="87E05258F3FDD07E" modified="2012-06-13" version="1">MARLKFPRRYVIVFLTFICTNVCYIERVGFSIAYTVSADAIEVNQANKGMILSMFYYGYVLSQIPGGWAAQRMGGRRVLLLSFLLWSLICGLIPLDPNRVVILVLSRLFVGVAQGFIFPAIHTVLAQWVPPQERSRSVSLTTSGMYLGAAGGMLFFPSLVKHMGPQSVFFVEAVLGVAWSLIWLKFSSEPPRTDLPKVAMPKVASREKIKAQAGGVVAPRTVKIPWRRIIFSLPVWAIVVNNFTFHYALYVIMNWLPTYFELALKLSLQDMGSSKMLPYFNMFIFSNIGGVVADHLITRRILSVTKTRKLLNTIGFVVSAVALMALPSFGTPSGTVICSSISLGFLALGRAGFAVNHMDVAPKFAGIVMGVSNTAGTLAGIVGVGLTGNILEAAKASNMDLTSSETWKTVFFVPGYLCIFSSVIFLIFSTGEKIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I1IU11</accession>
    <name>I1IU11_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100824857</name>
        <name evidence="8" type="ORF">BRADI_4g41527v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578839.1">
        <property type="nucleotide sequence ID" value="XM_003578791.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IU11"/>
    <dbReference type="FunCoup" id="I1IU11">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IU11"/>
    <dbReference type="EnsemblPlants" id="KQJ92068">
        <property type="protein sequence ID" value="KQJ92068"/>
        <property type="gene ID" value="BRADI_4g41527v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824857"/>
    <dbReference type="Gramene" id="KQJ92068">
        <property type="protein sequence ID" value="KQJ92068"/>
        <property type="gene ID" value="BRADI_4g41527v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824857"/>
    <dbReference type="eggNOG" id="ENOG502QVK2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009026_3_0_1"/>
    <dbReference type="OMA" id="RERKQFF"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IU11">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd17781">
        <property type="entry name" value="CBS_pair_MUG70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17782">
        <property type="entry name" value="CBS_pair_MUG70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06409">
        <property type="entry name" value="PB1_MUG70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF125">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 70 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="520"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="60"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="126"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="227"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="294"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="7">
        <location>
            <begin position="407"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ92068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="58756" checksum="6AA1E76F64681B46" modified="2012-06-13" version="1">MSSAAAAAPPPRRTRSRLQPSLSRKSDDPSTIAANGNGKTSPKLASPKHLPRERTVKKLRLSKALTIPEGTTVYDASRRMATRRVDAVLLTDAQGLLSGIVTDKDISTRVIAEGLRVEQTIMSKIMTRNPTYVTSDSLAIEALQKMVQGKFRHLPVVENGEVMAMLDIAKCLYDAIARLEKAAEQGSAIAAAVEGVERQLGGNFTAPYAFIETLRERMFKPSLSTIVTEGTKVAIVSPSDPVYVATQQMREFRVNSVVVTTGNTVQGIFTSKDVLMRVVSQNLSPELTLVEKVMTANPDCATLDTTILDALHIMHDGKFLHIPVLDREGQIAACLDVLQLTHAAIQLVEGGNGAANDVANSVMQRFWDSALALEPPDDEFDSRSEASLLMASEAGDGKSSIYPPIIGNSFAFKLQDRKGRVHRFTCGLESLDELMSSVRQRLSIVDEKDSIQLLYEDDEGDRVLLTTDTDLSGAVLHAKSSGLKVLKLHIDDESTSNIETVKPLQELAPPRRGGLSPAKVGLMAGAVALSGAAVMVYLKRSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1IY84</accession>
    <name>I1IY84_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glutamate decarboxylase</fullName>
            <ecNumber evidence="3 9">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100832399</name>
        <name evidence="10" type="ORF">BRADI_5g11640v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3 9"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579852.1">
        <property type="nucleotide sequence ID" value="XM_003579804.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IY84"/>
    <dbReference type="FunCoup" id="I1IY84">
        <property type="interactions" value="225"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IY84"/>
    <dbReference type="EnsemblPlants" id="KQJ82857">
        <property type="protein sequence ID" value="KQJ82857"/>
        <property type="gene ID" value="BRADI_5g11640v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832399"/>
    <dbReference type="Gramene" id="KQJ82857">
        <property type="protein sequence ID" value="KQJ82857"/>
        <property type="gene ID" value="BRADI_5g11640v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832399"/>
    <dbReference type="eggNOG" id="KOG1383">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019582_2_2_1"/>
    <dbReference type="OMA" id="VGWVFWR"/>
    <dbReference type="OrthoDB" id="5152799at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000022">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.160:FF:000001">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.50:FF:000001">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321:SF13">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ82857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="501" mass="55481" checksum="DC81E212534F1614" modified="2012-06-13" version="1">MVLAHVQSDEAAASAAVFASRYVQEPIPSFELGEKSISKDAAYQIIHDELLLDGSPRLNLASFVTTWMEPECDRLIIEGMNKNYADMDEYPVTTELQNRCVNIIARLFNAPLGAAETAVGVGTVGSSEAIMLAGLAFKRRWHNRRKAAGKPHDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGCYVMDPDKAVEMVDENTICVAAILGSTLTGEFEDVKRLNGLLAAKNKRTGWDTPIHVDAASGGFIAPFLYPELEWDFRLPLVKSINVSGHKYGLVYPGVGWVVWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQIIAQYYQFLRLGFEGYKNVMENCMESAKTLREGLLRTGRFDVISKEDGVPLVAFVFRGASARDGSLAFKLSASLRQFGWIVPAYTMPADLEHMTVLRVVVREDFGRPLAERFLSNVRMALNELDLADKGPVTKMRLTIELGPARGADEEASVRVVEREAVSAHGSVSLVTGKTKGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E4T2</accession>
    <name>A0A0Q3E4T2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_5g11315v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E4T2"/>
    <dbReference type="STRING" id="15368.A0A0Q3E4T2"/>
    <dbReference type="EnsemblPlants" id="KQJ82839">
        <property type="protein sequence ID" value="KQJ82839"/>
        <property type="gene ID" value="BRADI_5g11315v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82839">
        <property type="protein sequence ID" value="KQJ82839"/>
        <property type="gene ID" value="BRADI_5g11315v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E4T2"/>
    <dbReference type="OrthoDB" id="785127at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF4">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAR1" evidence="1">
        <location>
            <begin position="134"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="MULE transposase" evidence="2">
        <location>
            <begin position="383"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ82839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="477" mass="54371" checksum="9A79EE47FED95FD2" modified="2016-01-20" version="1">MLDLNEIPCDPDDAPQEGVEPAYCTPIAPVAVHEEVGESPATIRIADNEPLIVAPIAEYNTGVAEHNTIVAEDSTGVAEHNTIVAEDSTGVAEDTTRVACEAPADAEQELEYRSQPQEPFLGMRFDTLAGAKEHYNAYARMKGFSIKSNTSRWSAFTNLVEKHQFVCNKFRKPKPIDDVAGQTSKAGIYSTKVPREESSSDEEHRAARTEETLVMKKVVRKRKRESIKQTECKAKMTAKLIDGRWEVIFFVNEHNHELITKPSLTKYLLSHKNISPEEEDFLRILDDCNLQTGQMMQLMSRFYANGFIVPYKTKTISNFRFKIRAERKGDDMAETVSYFIAKQKEDLSFYFNVKMDEEDRVELLFWVDGAARKAYTESYHDCISFDTTYLTNQYNMPFAPFIGINCHGQSFMLGYGFMRDETTSSFDWLFQQYLDAMGGQAPLNIITDQDNAMRASIVNMFPNTVLRNCRWHIMKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EEC3</accession>
    <name>A0A0Q3EEC3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_5g22710v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin U/P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EEC3"/>
    <dbReference type="STRING" id="15368.A0A0Q3EEC3"/>
    <dbReference type="EnsemblPlants" id="KQJ84772">
        <property type="protein sequence ID" value="KQJ84772"/>
        <property type="gene ID" value="BRADI_5g22710v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ84772">
        <property type="protein sequence ID" value="KQJ84772"/>
        <property type="gene ID" value="BRADI_5g22710v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EEC3"/>
    <dbReference type="OrthoDB" id="77656at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EEC3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037485">
        <property type="entry name" value="PEX22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34126:SF9">
        <property type="entry name" value="OS04G0629000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34126">
        <property type="entry name" value="PEROXISOME BIOGENESIS PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22978">
        <property type="entry name" value="HAD_Pex22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="180"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="271"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ84772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="483" mass="53707" checksum="1EDF4B9826C35D7E" modified="2016-01-20" version="1">MDTETPPPPPPPPPPELGMVARAVQRLVARNDAAAAEGDHRPFGIINGMAAFEAAGRKGAARIGVGEYLERVHRYAGLEAECYVAAYAYLDMAAHRRPAAAVASRNVHRLLLACLLLASKVLDDFHHNNAFFARVGGVSNAEMNKLELELLTVLDFRVMLSRRLYDLYRDHLHKLDAQTTPADHVKAKQQQQQQQQPEELRSPDADEGDGEVDDDTHDVRHRKKLPNGGVLHDWSELMANGAGKKLRSLTTLAAIAITLVFAWKLLRAPQEQPRRPRRRVAPSSSNTSSRSRPGALASTDACSSADSRAHEAINQLFQPVNLTLEQLVRHKLSEGRRVTCRLLGVILEETTPEELQSHATVRSSVLEVLLEIAKFCDVYLMECILDDESEEKVLSALSEAGLFTSGGFVKDKVLFCSTENGRTSFVRQLEPDWHIDSSPEIVHQLSRFIKYQLHISPQQTERLAPNVFSSTSLEQFFGGLDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EMW9</accession>
    <name>A0A0Q3EMW9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g21834v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EMW9"/>
    <dbReference type="EnsemblPlants" id="KQJ88870">
        <property type="protein sequence ID" value="KQJ88870"/>
        <property type="gene ID" value="BRADI_4g21834v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88870">
        <property type="protein sequence ID" value="KQJ88870"/>
        <property type="gene ID" value="BRADI_4g21834v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EMW9"/>
    <dbReference type="OrthoDB" id="693277at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ88870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="15036" checksum="AE0C0D80E4832853" modified="2016-01-20" version="1">MDVPSYNCIMCDENVLEKRDHLFFHCKFSKTCWRYLCPNFSAKQNVHANVSHIKEELSVPFHVEIMTLCAWSIWKTRNDYISNNVIPSFYRCRRLFKEELNMVFHRSKRKKYVNFETWISLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F2Z4</accession>
    <name>A0A0Q3F2Z4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein-mitochondrial domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g01116v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F2Z4"/>
    <dbReference type="FunCoup" id="A0A0Q3F2Z4">
        <property type="interactions" value="1870"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3F2Z4"/>
    <dbReference type="EnsemblPlants" id="KQJ92852">
        <property type="protein sequence ID" value="KQJ92852"/>
        <property type="gene ID" value="BRADI_3g01116v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92852">
        <property type="protein sequence ID" value="KQJ92852"/>
        <property type="gene ID" value="BRADI_3g01116v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3F2Z4"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3F2Z4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057027">
        <property type="entry name" value="TPR_mt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23276">
        <property type="entry name" value="TPR_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="175"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="210"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="245"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="315"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="350"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="423"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="499"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Pentatricopeptide repeat-containing protein-mitochondrial" evidence="6">
        <location>
            <begin position="511"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="534"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ92852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="751" mass="82444" checksum="B12E452B994A192D" modified="2016-01-20" version="1">MARRHLLLPLLSRSPHAPTPLHLRHALCSSSSSPPPTPAIEPTPTVETPEEEAPSAAAAEEKPDTPPPRAEEPLHETILHMIRRRKWTTRLENSVRLLSPTLSAPLVHGVISSAAAANRADLALQFFRFAYRRAGFRPEPATFSLLIPVLASNRMLNHARCILLETMPAFSVSPDEATVAALVAAYGKARIPQEAVKLFRLMPELGITRTALSYNAVLKAILCRGREAMARRIYNAMIADGVAPDLSTYNTLIWGFGLSEKMEAAVRVFGDMKGHGVTPDVTTYNNLINAWVRNGDLESARKVFDEMPGAGFERNSVSYNVMIKGYVEAKKVEEAVGLFKEMGEKGLRSSEKTFAALMPGLCDDQGRTAEARKAVEDMAERRLTPKDKTVFLRLVTTLCKAGDLDGALEVHKKSGQFKHVLVDPRQYGVLMQSLCAGGKCDGAVEVLDELLEKGTLLSPKSPVLEAPAYNPVIEYLCNNGNTKKAETFFRQLMKKGVDDKLAFNSLIRGHAKEGVLEAAQEILAIMTRRGVPTDPISHTLLVDSFLKKNEPADAKTALDSMMEQGHLPSPALFKSVMVALFDDGRVQTASRVMRSMIEKGVTENMDMAHKILEALFMRGHVDEAIGRVNLMVENGCMPDLDKLLVALCQKDKVMEAHKLADFALDRDFEVSFSTYDKVLEALYTEEKTLPAYSMLCKIKHKGGVVDQKGCDALMDSLKAEGYSKQADILSRILVESGSSTSKRGKKVAMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FCJ4</accession>
    <name>A0A0Q3FCJ4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription repressor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ovate family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_3g28000v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional repressor that regulates multiple aspects of plant growth and development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FCJ4"/>
    <dbReference type="FunCoup" id="A0A0Q3FCJ4">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FCJ4"/>
    <dbReference type="EnsemblPlants" id="KQJ96966">
        <property type="protein sequence ID" value="KQJ96966"/>
        <property type="gene ID" value="BRADI_3g28000v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96966">
        <property type="protein sequence ID" value="KQJ96966"/>
        <property type="gene ID" value="BRADI_3g28000v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FCJ4"/>
    <dbReference type="OrthoDB" id="1928390at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FCJ4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025830">
        <property type="entry name" value="DNA_bnd_dom_ovate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038933">
        <property type="entry name" value="Ovate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006458">
        <property type="entry name" value="Ovate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01568">
        <property type="entry name" value="A_thal_3678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057:SF200">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR OFP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13724">
        <property type="entry name" value="DNA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04844">
        <property type="entry name" value="Ovate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51754">
        <property type="entry name" value="OVATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OVATE" evidence="8">
        <location>
            <begin position="161"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ96966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="228" mass="26204" checksum="4349E0FF0466308D" modified="2016-01-20" version="1">MSNHRKFRLSHLMPNSWFYKITEMKRPRPPSQSTAAATRSSKRPSNHYCHRATTPKPLPLSQHQSYYTDLQEKEVFPEKLHLSPLHLNPKATDIQFPKDHHHQSPTPASTGARCVHRTTITRSVACPSSPRLRSRKLHDQSSSCRVSTGHQRSSAARSFAVLIASRNPSRDFRESMVEMIIENDLRAPNDLEGLLECYLSLNSREYHRVIKEVFEAIWLQIADDSIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FT89</accession>
    <name>A0A0Q3FT89_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g01041v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT69822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT69823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FT89"/>
    <dbReference type="FunCoup" id="A0A0Q3FT89">
        <property type="interactions" value="702"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK02366">
        <property type="protein sequence ID" value="KQK02366"/>
        <property type="gene ID" value="BRADI_2g01041v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK02367">
        <property type="protein sequence ID" value="KQK02367"/>
        <property type="gene ID" value="BRADI_2g01041v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69822">
        <property type="protein sequence ID" value="PNT69822"/>
        <property type="gene ID" value="BRADI_2g01041v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69823">
        <property type="protein sequence ID" value="PNT69823"/>
        <property type="gene ID" value="BRADI_2g01041v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02366">
        <property type="protein sequence ID" value="KQK02366"/>
        <property type="gene ID" value="BRADI_2g01041v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02367">
        <property type="protein sequence ID" value="KQK02367"/>
        <property type="gene ID" value="BRADI_2g01041v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69822">
        <property type="protein sequence ID" value="PNT69822"/>
        <property type="gene ID" value="BRADI_2g01041v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69823">
        <property type="protein sequence ID" value="PNT69823"/>
        <property type="gene ID" value="BRADI_2g01041v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FT89"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK02367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="175" mass="18440" checksum="3C928B7D8F7D36C1" modified="2016-01-20" version="1">MSLSGSCSCNYRPMILVYLSSRLCRPATYQPFNTYSLQQGASASPTKGFSRRRRSGELARRKMPSLRSALLLAAAAVWLWWSAAGALADDSDGGASFPFLAPPTSPFPFCPPRPATPSSTAPFPWAPAPPRAAQFPQDPGFLAAAAACRGTTAMAAAAAWVTVVALCSAFLVPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FW65</accession>
    <name>A0A0Q3FW65_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100830414</name>
        <name evidence="14" type="ORF">BRADI_2g01360v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DExH box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567582.1">
        <property type="nucleotide sequence ID" value="XM_003567534.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FW65"/>
    <dbReference type="FunCoup" id="A0A0Q3FW65">
        <property type="interactions" value="804"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FW65"/>
    <dbReference type="EnsemblPlants" id="KQK02429">
        <property type="protein sequence ID" value="KQK02429"/>
        <property type="gene ID" value="BRADI_2g01360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830414"/>
    <dbReference type="Gramene" id="KQK02429">
        <property type="protein sequence ID" value="KQK02429"/>
        <property type="gene ID" value="BRADI_2g01360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830414"/>
    <dbReference type="OrthoDB" id="5600252at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FW65">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000059">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000480">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000526">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF229">
        <property type="entry name" value="DEXH-BOX ATP-DEPENDENT RNA HELICASE DEXH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="354"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="608"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="11">
        <location>
            <begin position="1149"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="17"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="81"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1120"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1213"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1220"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1236"/>
            <end position="1247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK02429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1247" mass="139685" checksum="577451B634F55CA9" modified="2016-01-20" version="1">MLLASSVLRGRLRPLRRPRPLIMPSPLFLSRNPNPSPGSNGASLLPSPSPHHSSSAAMSTGGGGGVYVPPMRRLRSVIASTNGSLAPPPLAQPAWTPEWRADGRSNSPPSPPQQQQRRAAPLPPRPPPPQQPPLRQQSAGYARYAYDDFSEDESDREMDRSSASSKGASTLDNVDEWKWKLHMLLRNDDEQEIISREKKDRRDFEQLAQLADRMALHSRQYSRIIVFSKVPLPNYRSDLDDKRPQREVSIPSGLQREVDALLADYLARKRTNSGNFPNAAFSRSSSTDSFVTDESFYDQPDNQASANVVLERIQKRKSLQLRNQQAAWQESNDGQSMMEFRRSLPAYKERQSLLDAISRNQVVVVSGETGCGKTTQLPQYILESEIDAARGATCSVICTQPRRISAITVSERVAAERGEKIGESVGYKVRLEGMRGRDTRLLFCTTGVLLRRLLVDRSLKGVTHVIVDEIHERGMNEDFLLIVLKDLLPRRPELRLVLMSATLNADLFSSYFGGAPMIHIPGFTYPVRSRFLEDILEVTGHRLTSYNQIDDYGQEKSWKMQKQAIRKRKSQIASVVEDAVKAADLRDYSSRTRDSLSCWNPDSIGFNLIENVLCHICQKERDGAVLVFMTGWDDINALKEQLQANPLLGDPNKVLLLACHGSMPSSEQKLIFEKPEAGLRKIVLATNLAETSITINDVVFVVDCGKAKETSYDALNNTPCLLPTWISKASARQRRGRAGRVQSGECFHLYPQCVYNAFADYQLPELLRTPLQSLCLQIKSLRLGSISEFLSRALQSPESLSVQNAIEYLKVIGAFDQNEELTVLGRHLSMLPVEPKLGKMLILGAIFNCLDPILTIVSGLSVRDPFMTPFDKKDLAESAKLQFSCRDYSDHLALVRAYEGWREAERDRNGYDYCWKNFLSVQTLKALDSLRRQFVFLLKDTGLIDENMTRCNKWSRDENLVRAVICAGLYPGVSSVVNKEKSISLKTMEDGQVMLYSSSVNGKEAKIPFPWLVFNEKVKVNSVFLRDSTAISDSILLLFGGNIQQGGLDGHLKMLGGYLEFFMSRDLASTYLNLKSELEDFIHCKLQNPKMDIQTSEELLSAVRLLVTEDPCSGRFVYGRQEPRSKKAKTMTSLASASMDRGGGHGGDNPKNQLQTLLTRAGHGNPSYKTKQIKNSLFRSTVEFNGMQFVGQPCANKKLAEKDAAGEAINWLTGGGAPSDSRDPQDADHMSMLLKPTRRKRHHFRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G6U9</accession>
    <name>A0A0Q3G6U9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g32981v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G6U9"/>
    <dbReference type="FunCoup" id="A0A0Q3G6U9">
        <property type="interactions" value="701"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK07087">
        <property type="protein sequence ID" value="KQK07087"/>
        <property type="gene ID" value="BRADI_2g32981v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07087">
        <property type="protein sequence ID" value="KQK07087"/>
        <property type="gene ID" value="BRADI_2g32981v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3G6U9"/>
    <dbReference type="OrthoDB" id="610439at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3G6U9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34838:SF3">
        <property type="entry name" value="OS08G0142100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34838">
        <property type="entry name" value="OS08G0142100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999563" description="Pectinesterase inhibitor domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK07087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="18096" checksum="F69F166D6C2C6151" modified="2016-01-20" version="1">MKRAAAAAAAMILLAMAPIPMALACNNVPSMDIDAACLKATTSQQLYQLCRYTLRHADVATGDVTGYAAAAAQLAKLSYDSALGTIDGLLAGKPLPGGEKAAYQRCRARYADARKILSGVFGAGLRREYVDATAEVAACAAALAAFRSSPLYSVNAADRDRALLASQLGALVVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G973</accession>
    <name>A0A0Q3G973_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g38275v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G973"/>
    <dbReference type="EnsemblPlants" id="KQK07895">
        <property type="protein sequence ID" value="KQK07895"/>
        <property type="gene ID" value="BRADI_2g38275v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07895">
        <property type="protein sequence ID" value="KQK07895"/>
        <property type="gene ID" value="BRADI_2g38275v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3G973"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK07895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="83" mass="9159" checksum="1CD214BE543C91F5" modified="2016-01-20" version="1">MYAGCSPLHCHALAAPSSFPLATPPPRWRDALQDRDGARHRVGHGEVSSQSEADLQRRRSSCRTRPPGPIVLQRLLDLMCSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GP57</accession>
    <name>A0A0Q3GP57_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100840261</name>
        <name evidence="18" type="ORF">BRADI_1g02210v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236383.1">
        <property type="nucleotide sequence ID" value="XM_010238081.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GP57"/>
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        <property type="gene ID" value="BRADI_1g02210v3"/>
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    <dbReference type="Gramene" id="KQK12213">
        <property type="protein sequence ID" value="KQK12213"/>
        <property type="gene ID" value="BRADI_1g02210v3"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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            <begin position="1"/>
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    <feature type="chain" id="PRO_5043129162" description="Protein kinase domain-containing protein" evidence="16">
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            <begin position="888"/>
            <end position="898"/>
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        <location>
            <position position="633"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="950" mass="103634" checksum="CF922089BA7CF3FC" modified="2016-01-20" version="1">MGSTDGIVVLSYMKLVVLVWSLASTTAVVSSAAPPSSPSTSTLAHCPKTCGDVSISYPFGVGPGCFRRGGGFELICDTATQPPKLFLGNTTTRVTGLFPSGTVLASIVQIIPMVPGISTYNLSWDSPGKNLNIMTYNYLAFLGCGFGVYLFDPDTGGVIGHCTVKCAPMATMLLATEGGICNGMGCCTVTFPVPFRGFRVTIVKDEETVVPPPFANITTIKAFLTLYSYKFSIADLLSDKINANTVGASSAYLSTVITDKPNCAKALLDDKTSYACSSSNCVDVANGGYSCSCSGTSDDGNPYLLDDCKQEYNPTPKVNCSRSCGSTNIPFPFGLESGCYARRRFQMNCASNRLLIGRPPVKYEVTNISLDDGLLYVNKLSEFNDANTKFLSVYYGASGYFGQQLVYGLDKSDLSEEYGVWRWSATNLTCKNAKNKSSYACRSTNSECLGVTHGKVYIGYRCKCSSGFEGNPYVQNGCTDIDECQIPNKCNGKCYNLKGSYHCCPQAMSFDTIRNQCTINKRQNLLLGVATGIGSGLGVLALALIAVVLVRRWKRSTQKKIRRAFFRKNKGLLLEQLISSTSGGSVTHSTRIFSLDELEKATNNFDSTRILGHGGHGTVYKGILSDQRVVAIKRSKMVEQSEIDQFVNEVSILSQVIHRNVVKLFGCCLESEVPLLVYEFISNGTLHDLLHGGDPCAKCLLTWDDRTRIALEAAGALAYLHSSAAMPIFHRDVKSTNILLDDTFATKVSDFGASRSISIDQTHVVTIVQGTFGYLDPEYYYTGQLTEKSDVYSFGVILVELLTRKKPIFLDSFGDKQNLCHYFLRGLRDDTVMDIIDAQIVEEAVRSEIDEIVSVAEACLRTKGEKRPKMKEVELRLQMLRARRPSRTCKEELGRGSETKPLLPTPAKSKSASLSTAKNVVELGANPASQALSRCYSMEQDMISSAEFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H252</accession>
    <name>A0A0Q3H252_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">X8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_1g31870v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17000.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H252"/>
    <dbReference type="STRING" id="15368.A0A0Q3H252"/>
    <dbReference type="EnsemblPlants" id="KQK17000">
        <property type="protein sequence ID" value="KQK17000"/>
        <property type="gene ID" value="BRADI_1g31870v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17000">
        <property type="protein sequence ID" value="KQK17000"/>
        <property type="gene ID" value="BRADI_1g31870v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H252"/>
    <dbReference type="OrthoDB" id="417697at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H252">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044788">
        <property type="entry name" value="X8_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044">
        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF42">
        <property type="entry name" value="OS06G0665200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033724927" description="X8 domain-containing protein" evidence="9">
        <location>
            <begin position="32"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="10">
        <location>
            <begin position="100"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK17000.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="188" mass="20073" checksum="B89F2CE284D67F39" modified="2018-03-28" version="2">MELMMMMVIKKHPMILLALIMSIQHDSKANGASVTITRDWRYYQSYLANPPYKYQIGVGGSPSPAPAPAPPPYCAYPPPPPPPASPRGAAPRQPARTQGLWCVANPTVESEEVQAAMDYACGSGADCDAAAPGGPCFLPDTLMAHASHAFNSYWQRAKVAGGTCDFAGAAMLITRDPSESSLFFLRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H798</accession>
    <name>A0A0Q3H798_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g24586v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H798"/>
    <dbReference type="FunCoup" id="A0A0Q3H798">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3H798"/>
    <dbReference type="EnsemblPlants" id="KQJ89274">
        <property type="protein sequence ID" value="KQJ89274"/>
        <property type="gene ID" value="BRADI_4g24586v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89274">
        <property type="protein sequence ID" value="KQJ89274"/>
        <property type="gene ID" value="BRADI_4g24586v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H798"/>
    <dbReference type="OrthoDB" id="684435at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31704:SF37">
        <property type="entry name" value="HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31704">
        <property type="entry name" value="MYB/SANT-LIKE DNA-BINDING DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ89274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="149" mass="17500" checksum="16C92DEB50D983A5" modified="2016-01-20" version="1">MEKGKTGKATWDAVAHRVFLDVCIKEVEANNRPTQCLNDVGYANLISKFNEHTKRNYDRKQMKNRWETLKKDYNTWKTLTQRASGIGRDPNTHTIAASDEWWEKEIQRVPEASKFRNAPLQDEEKMSIIFDKNCVTNEHARVPVYRRMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HFC1</accession>
    <name>A0A0Q3HFC1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100834159</name>
        <name evidence="6" type="ORF">BRADI_1g59505v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HFC1"/>
    <dbReference type="FunCoup" id="A0A0Q3HFC1">
        <property type="interactions" value="1008"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3HFC1"/>
    <dbReference type="EnsemblPlants" id="KQK21229">
        <property type="protein sequence ID" value="KQK21229"/>
        <property type="gene ID" value="BRADI_1g59505v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21229">
        <property type="protein sequence ID" value="KQK21229"/>
        <property type="gene ID" value="BRADI_1g59505v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="599416at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000048">
        <property type="entry name" value="Protein strictosidine synthase-like 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF114">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE CONSERVED REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="5">
        <location>
            <begin position="136"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK21229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="319" mass="35211" checksum="7195BE5735CC758D" modified="2016-01-20" version="1">MAVAVASFDASRSQRLPLPRGYLRGPESVAFDGEGHGPYSGVSDGRILKWNGDKIGWSTYAYGPDYSSEACTASKLRSETVTESHCGRPLGLQFHHKSGNLYIADAYKGLMWVGPSGGEATVLVNQVDGAPLRFTNGVDVDQITGQVYFTDSSMNYQRSQHEMVTRTGDSTGRLMRYDPRTNDVTTLQSGITYPNGVSISHDRTHLVFASTGPCKLLRYWIKGPEAGKTEPFADLPGYPDNVRRDRRGGYWVALHREKNELPFEFGSHLLAVRVGPNGKVLEEMRGPKSVRPTEINERGNGKYYMGSVELPYVGVVTHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HFY0</accession>
    <name>A0A0Q3HFY0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_4g43346v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HFY0"/>
    <dbReference type="FunCoup" id="A0A0Q3HFY0">
        <property type="interactions" value="664"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92394">
        <property type="protein sequence ID" value="KQJ92394"/>
        <property type="gene ID" value="BRADI_4g43346v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92394">
        <property type="protein sequence ID" value="KQJ92394"/>
        <property type="gene ID" value="BRADI_4g43346v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HFY0"/>
    <dbReference type="OrthoDB" id="696608at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31989">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 82-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="7"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ92394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="335" mass="36434" checksum="571C641FDDEB95E0" modified="2016-01-20" version="1">MELDMEVFQLSRFDLSPLEAVTYYLPRLLSGETLHGAERLIHRADIYGNLEPKDLAAAFPPAPKAERTGDRFFLTLCKRQKGSRSAHAAGAGTTLEVVDHAGVKKGEVKKLSFKKGIKKEKESMGWVMEEYHCLLPEAVVADGEMVLCKIHLSPTACDEARQESAAYLAGTQEQEDQPAPMAVTAKRPAPAASDDPPCAPKRARVAVAEADHSQDMDGFRSPLEDLLADEPADELLGGATDEDQEEEAAALPAGETEQQIVDFDFDLPVLDEDALSEVILSLDEDLPIASDEDFDFKFPTAEELNAMLSNVGDWPLSFAPDGDLLRFPGLPAAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HP47</accession>
    <name>A0A0Q3HP47_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC100834508</name>
        <name evidence="24" type="ORF">BRADI_3g15660v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571417.2">
        <property type="nucleotide sequence ID" value="XM_003571369.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HP47"/>
    <dbReference type="FunCoup" id="A0A0Q3HP47">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3HP47"/>
    <dbReference type="EnsemblPlants" id="KQJ95194">
        <property type="protein sequence ID" value="KQJ95194"/>
        <property type="gene ID" value="BRADI_3g15660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834508"/>
    <dbReference type="Gramene" id="KQJ95194">
        <property type="protein sequence ID" value="KQJ95194"/>
        <property type="gene ID" value="BRADI_3g15660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834508"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HP47">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.510">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129258" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="26"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="302"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="208"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQJ95194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="625" mass="68747" checksum="D507EF4D9CE18505" modified="2016-01-20" version="1">MAAPRWALWALLLLPLLHPATRVLANTEGDALHNLRTNLNDPNNVLQSWDPTLVNPCTWFHVTCNNDNSVIRVDLGNAALSGTLVPQLGQLKNLQYLELYSNNISGTIPSELGNLTNLVSLDLYLNNFTGPIPDSLGNLLKLRFLRLNNNSLSGTIPKSLTAITALQVLDLSNNKLSGEVPSTGSFSSFTPISFGNNPALCGPGTSKPCPGAPPFSPPPPYNPPTPVQSPGSSSSSTGAIAGGVAAGAALLFAVPAIGFAYWRRRKPEEHFFDVPAEEDPEVHLGQLKRFSLRELQVATDTFSNKNILGRGGFGKVYKGRLTDGTLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASRLRERGPSEPPLDWQTRRRIALGSARGLSYLHDHCDPKIIHRDVKAANILLDEDFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGIMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKERRLEMLVDPDLQEAYIDVEVESLIQVALLCTQGSPTERPKMSEVVRMLEGDGLAERWEEWQKVEVVRQEVELGPHRNSEWIVDSTDNLHAVELSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I4F8</accession>
    <name>A0A0Q3I4F8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC112271549</name>
        <name evidence="2" type="ORF">BRADI_3g51520v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_024460865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I4F8"/>
    <dbReference type="EnsemblPlants" id="KQK00739">
        <property type="protein sequence ID" value="KQK00739"/>
        <property type="gene ID" value="BRADI_3g51520v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112271549"/>
    <dbReference type="Gramene" id="KQK00739">
        <property type="protein sequence ID" value="KQK00739"/>
        <property type="gene ID" value="BRADI_3g51520v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="584049at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3I4F8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK00739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="95" mass="10082" checksum="148AA85740924365" modified="2016-01-20" version="1">MEDLGVLARGEGWTEERHAAFLDGVELSFVQEVLGGAGAGVSDERRASRRISRRGAPPPPAGGRGQLPLDRPLPDSAVESNRRGPSNRPAARDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I4M1</accession>
    <name>A0A0Q3I4M1_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g17796v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I4M1"/>
    <dbReference type="EnsemblPlants" id="KQJ95563">
        <property type="protein sequence ID" value="KQJ95563"/>
        <property type="gene ID" value="BRADI_3g17796v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95563">
        <property type="protein sequence ID" value="KQJ95563"/>
        <property type="gene ID" value="BRADI_3g17796v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3I4M1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ95563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="171" mass="16657" checksum="596412D2EFEF9008" modified="2016-01-20" version="1">MEDFRLHCRRCTPPQGRSLVPWARSRKDRVRRQAAMEMSCAGREVTDRGGGTEMETVPYKGERGHTVAGVGAATAGVGATETVGVVDGVGVAGAEGAGAGAAGAEGAGAEVAGAGAAGGGGGVEAARRGACGTRGFPLLVVRCDPPAPDLSFCDMAAAGKGGGGGIGQGFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IQV5</accession>
    <name>A0A0Q3IQV5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_2g03012v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IQV5"/>
    <dbReference type="EnsemblPlants" id="KQK02676">
        <property type="protein sequence ID" value="KQK02676"/>
        <property type="gene ID" value="BRADI_2g03012v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02676">
        <property type="protein sequence ID" value="KQK02676"/>
        <property type="gene ID" value="BRADI_2g03012v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IQV5"/>
    <dbReference type="OrthoDB" id="1050628at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF909">
        <property type="entry name" value="OS11G0673900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="Disease resistance protein winged helix" evidence="3">
        <location>
            <begin position="94"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="212"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="740" mass="83517" checksum="68F0E0DAE96017BF" modified="2016-01-20" version="1">MGATLEELNIDDEKEVQHLRNIGNEIVRKCGRLPLAIKVTASALTCRDLTENEWKRFLGKYSQSILSDGTEAALYLSYDELPHHLKQCFLYCALYTEDSIIELRIVAKLWIAEGFVVEQQGQVLEDIAEEYYYELIHRNLLQPCGKSYSQAKCTMHDLLRQLASNISREECFIGDVETLSGASMSKLRHVTAVTKKEMLVLPSIDKVEVKVRTFLTVHGPWRLEDTLFKRFLLLRVLVLNYSLVQSIPDYIGKLIHLRLLNLDYTAISCLPKSIGFLKNLQVLSLRFCKDLHSLPLTMTQLCNLRSLLLLGTAISKVPKGIAKLKFLNEIEAFPVGVGCDNADLQDGWKLEELSSVSQIRYLHLVKLERAAHCSPNTVLTDKKHLKILILEWTELGEGSYSENDVSNTENVLEQLRPPGNLENLWIHGFFGRRYPTWFGTTFLSSLMHLILSDLRSCVDLPPLGQIPNLKFLRIEGAYAVTKVGPEFVGCRKGDSACNELLALPKLEILVIVDMPNWEDWSFLGEDESADAERGEDGAAEICKEDAQSARLQLLPRLVVLELLYCPKLSALPRQLGEGTATLKELALVGANNLKAVEDFPLLSELLYIQNCEGLERVSNLPLVSEVRVFHCPNLGCVEGLGSLQRLVLGEDMHEVSSRWLPELQNQHQQLHDGEDLDVFVVPRKLQDSWMATKSQAHHLDGQSTRHTKSMSNARCLRQCYKIKPVYSQAPVLISILEVPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J3Z0</accession>
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        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g02590v3</name>
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    <organism evidence="5">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
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        <text evidence="1">Belongs to the MAP65/ASE1 family.</text>
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        <property type="gene ID" value="BRADI_3g02590v3"/>
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    </feature>
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        <location>
            <begin position="618"/>
            <end position="642"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ93072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="665" mass="74726" checksum="6E5C960A0FF32C0F" modified="2016-01-20" version="1">MASQPLARLVPPSARALFLSHTHIHSTTRARNPSLPSIDRSPSPEEACGGGRHAPRTSCMRTTGGGQQRKRKTVSSSAPSGRRRNRPADPARSRNKSRPPPPAIDRTRKFRPGSMEPRREAVLRELGDAWDEIGEAEADRREALRALEEGCLALYRAELARLRQRTARLRRDVADAVAELAAIRAALGDTVQSSSANSSGGGRSRSLSLKEELGAIAPELEEMRRRRDERRRLFQEVTDVIDRIEQEMYSTNSNAAADDDGDLTMSKLQELRSRLGDLQSEKENRIKKVAELTSSLHSSSLVLGMDSGETDLEEGDISDCRIARLLSEIEHLREIKRDRMQKLQDLVAAMFELWNLMDTPPEEQSRFQDVACNIAASEEEITEPGALSVGFIANVEAEVVRLESLKERRMKDLLLKKRDELREIRRRTRIAAAEEDDDDGDGGGGGALLVFDAINDDTERSMILERLEAQISEAKDEEFSRKEVLERMEKWQAALEEESWLEEYNRNENRYSVGKGMHLMLKRAEKARALVNKMPAMAEALIAKVLAWEKERGEKFEYDGEGLLDMLEEYDNTRKEKEHERKRQRDQRKSQGQNRVESPVARALPKSIKNVTRTLSMGRSGSRKVAVSSSSPLSSSRPTTPSFLKSPMSPRGSDDGQMMASGPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="1">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="2">cv. Bd21</strain>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="EMBL" id="KQK08292.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK08292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JBA5</accession>
    <name>A0A0Q3JBA5_BRADI</name>
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        </submittedName>
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        <lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">Bd21</strain>
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        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
        </source>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ95584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JR81</accession>
    <name>A0A0Q3JR81_BRADI</name>
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        <recommendedName>
            <fullName evidence="8">IBB domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ORF">BRADI_1g17170v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JR81"/>
    <dbReference type="FunCoup" id="A0A0Q3JR81">
        <property type="interactions" value="903"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3JR81"/>
    <dbReference type="EnsemblPlants" id="KQK14548">
        <property type="protein sequence ID" value="KQK14548"/>
        <property type="gene ID" value="BRADI_1g17170v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14548">
        <property type="protein sequence ID" value="KQK14548"/>
        <property type="gene ID" value="BRADI_1g17170v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="29145at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JR81">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K7M6</accession>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <dbReference type="EMBL" id="KQK06849.1"/>
        </source>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK06849"/>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K7X8</accession>
    <name>A0A0Q3K7X8_BRADI</name>
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        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">Bd21</strain>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="9">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="10">cv. Bd21</strain>
        </source>
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            <location evidence="1">Nucleus</location>
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        <property type="protein sequence ID" value="KQK20580"/>
        <property type="gene ID" value="BRADI_1g55446v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK20580"/>
        <property type="gene ID" value="BRADI_1g55446v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="652253at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K7X8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020983">
        <property type="entry name" value="Basic_leucine-zipper_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46408">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46408:SF4">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bZIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="109"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="134"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="65"/>
            <end position="95"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK20580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="310" mass="34338" checksum="740C9803B92FB62E" modified="2016-01-20" version="1">MNCAKLLHDATIKQPSCFDLNFWHVRPQASRSSQSNTTSNQGSQNYIGDNEILVQQMSGGGQYGGTSPNLAQNPDVSVRQVSSPLREQSPSTNNIEGEAETMGNMNFSAEKVKMRRESNRISARLSRYKKATQMQNLQHQLSLLEAENKYLVKRQADLIQKYSSAVIDNRVLKANVETLETKVKLVEEIIKRFTSTHDVPQVVSSLTSLGFPLSASPSNGAHETFVPTQNTPFNYFTSVTTNGGVNNIYTPEATSTFQIQDPVALLQMQSESSLEHLQRRVCDSAPSSSVLAPQEVTSFNPNEFINMGMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3L0R8</accession>
    <name>A0A0Q3L0R8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100828649</name>
        <name evidence="4" type="ORF">BRADI_1g31430v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560397.2">
        <property type="nucleotide sequence ID" value="XM_003560349.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3L0R8"/>
    <dbReference type="FunCoup" id="A0A0Q3L0R8">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16921">
        <property type="protein sequence ID" value="KQK16921"/>
        <property type="gene ID" value="BRADI_1g31430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828649"/>
    <dbReference type="Gramene" id="KQK16921">
        <property type="protein sequence ID" value="KQK16921"/>
        <property type="gene ID" value="BRADI_1g31430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828649"/>
    <dbReference type="OrthoDB" id="617794at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3L0R8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF35">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="At1g61320/AtMIF1 LRR" evidence="3">
        <location>
            <begin position="133"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK16921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="58774" checksum="BCBD645A7EC304BB" modified="2016-01-20" version="1">MSSPPSAAAPASPSPATSSHPPPSGSDGPPVHSKIAPDYGLISVPTRMRSHHHRQENSSPGGKRTRFPGPELPEDIWCHIHSLMPMRDAAHAACVSRAFLRSWRCHPNLVFDRKTLGCRFRTVWDFESKVDDIVKKHSGIGVKTFSLEWHYGINAKACSYLDSWLQMANAPGIEELTLNTYSIYSRGTEYNFPCSLLSDDGRGSSIRYLRLAGCSFRPTVELGCSLRSLTTLNLCDVRITRDQLGCLLSGCAALERLELRRCSEITSLKIPSLLKHLSHLQVFGCRRLRMIENEAPNICSFLFWGLQVQLCLGQLLQLKDLELCCTSTTTVCYARQELPSVAPNLETLTIRSYHQMVSTAMLPSRLLHLKYLSIYLSGDYDYFSLVSFLDASPSLETFILNVPSWEDLAEDSVYREPLHLRRMPAYHHDKLRTVKISRFFHAKSLVELTRHILENAPSLESLTLDTTHGVLWCSTSKFGACYPLRTPLEPHKAALAIRTYIEEKVPSTVVFNVVEPCSRCHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3L5C0</accession>
    <name>A0A0Q3L5C0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100830966</name>
        <name evidence="10" type="ORF">BRADI_4g13871v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3L5C0"/>
    <dbReference type="STRING" id="15368.A0A0Q3L5C0"/>
    <dbReference type="EnsemblPlants" id="KQJ87845">
        <property type="protein sequence ID" value="KQJ87845"/>
        <property type="gene ID" value="BRADI_4g13871v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87845">
        <property type="protein sequence ID" value="KQJ87845"/>
        <property type="gene ID" value="BRADI_4g13871v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="9973183at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3L5C0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23812">
        <property type="entry name" value="UBCc_ScPEX4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000044">
        <property type="entry name" value="protein PEROXIN-4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="3"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ87845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="157" mass="17696" checksum="C4A721DBD4F3CA29" modified="2016-01-20" version="1">MTASRARLFKEYKEVQREKSADPDILLLCDDSNIFKWTALIKGPSETPFEGGVFELAFSIPEQYPLLPPQVRFLTKIFHPNVHFKTGEICLDILKSAWSPAWTLQSVCRAIIALMAHPEADSPVNCDFGNLLRSGDIRGYQSVARIYTRLAAVPKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LIS5</accession>
    <name>A0A0Q3LIS5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycosyl hydrolase family 43 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC104581847</name>
        <name evidence="6" type="ORF">BRADI_1g71160v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
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                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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        <source>
            <strain evidence="7">cv. Bd21</strain>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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        <location>
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            <end position="43"/>
        </location>
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            <begin position="1"/>
            <end position="10"/>
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    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="56" mass="6043" checksum="318AB335BB432312" modified="2016-01-20" version="1">MPRDADEEGYPGEVKDGAGRGSNNLVENGKQKKEAQVENGCHTKAWQHATSCLGED</sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
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</entry>
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            <fullName evidence="2">RNase H type-1 domain-containing protein</fullName>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">Bd21</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK13390.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3N9L0"/>
    <dbReference type="EnsemblPlants" id="KQK13390">
        <property type="protein sequence ID" value="KQK13390"/>
        <property type="gene ID" value="BRADI_1g09835v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13390">
        <property type="protein sequence ID" value="KQK13390"/>
        <property type="gene ID" value="BRADI_1g09835v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3N9L0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
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        <property type="entry name" value="RNase_H-like_EbsB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074">
        <property type="entry name" value="BNAC02G40300D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074:SF73">
        <property type="entry name" value="OS04G0448401 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="40"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK13390.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="151" mass="16045" checksum="45967B64C3CCE29E" modified="2018-03-28" version="2">MTTHHDLESSSPRTTVAGAPRPREQPKRWFPPPSGHAKINVDAALSKLGNGGSVDAVCRDETGFLLGSSALVFPGISDPVTLEALACREALALAEDLQVTRLRVASDCLEEIKQTSSCFESVSFVHERRTSNLDAHGLASASASLPVGRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3P4W5</accession>
    <name>A0A0Q3P4W5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g17126v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3P4W5"/>
    <dbReference type="EnsemblPlants" id="KQJ83841">
        <property type="protein sequence ID" value="KQJ83841"/>
        <property type="gene ID" value="BRADI_5g17126v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83841">
        <property type="protein sequence ID" value="KQJ83841"/>
        <property type="gene ID" value="BRADI_5g17126v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3P4W5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3P4W5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ83841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="237" mass="25398" checksum="7E4FBFFD0B2247A0" modified="2016-01-20" version="1">MTCTTPSLSLLIPHGRTLLISLSPSWTVASLSPSPPFLSHSCPAPSPPRSHLARCPRHQGAVLYHCTGLLQSQTPPRRQGAVAAPPSTNAGLRHAAKELLLYCPPPTPDSAVPPRSCRGSSRASTLLDPASDCPAAADPVGRRPLPRRMSVSRSRCSLSSLTHLHRRAPLPFYVSSPTPTAARSPSAPMRMSRPGGSSGRAGIPDLSHRRGEPEPQPSPSWSRNRSHHVVEQLQIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RDX0</accession>
    <name>A0A0Q3RDX0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100832034</name>
        <name evidence="6" type="ORF">BRADI_2g59937v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010234486.3"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3RDX0"/>
    <dbReference type="EnsemblPlants" id="KQK11394">
        <property type="protein sequence ID" value="KQK11394"/>
        <property type="gene ID" value="BRADI_2g59937v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832034"/>
    <dbReference type="Gramene" id="KQK11394">
        <property type="protein sequence ID" value="KQK11394"/>
        <property type="gene ID" value="BRADI_2g59937v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832034"/>
    <dbReference type="OrthoDB" id="1920276at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RDX0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33400:SF6">
        <property type="entry name" value="HOMEOBOX PROTEIN LUMINIDEPENDENS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33400">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="68"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="3">
        <location>
            <begin position="70"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="408"/>
            <end position="478"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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            <end position="948"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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            <begin position="410"/>
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        </location>
    </feature>
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        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
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            <begin position="531"/>
            <end position="549"/>
        </location>
    </feature>
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        <location>
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            <end position="983"/>
        </location>
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            <end position="1070"/>
        </location>
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            <end position="1116"/>
        </location>
    </feature>
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        <location>
            <begin position="1126"/>
            <end position="1154"/>
        </location>
    </feature>
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        <location>
            <begin position="1158"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK11394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1167" mass="128083" checksum="964A6D4038EA9B62" modified="2016-01-20" version="1">MELVPFKPAAGALVEAVGGGSIPAMVAAQQEQLHAQVGQLQRLVVAQCRLTGVNPLAQEMAAGALSIKIGKKPRDLLNPKAVKIMQSIFALKDNIGKRETREISALCGVTVTQVREFFASQRTRVRKVVRLSREKALKIEALEALEALEAPNGVCSMSTEQTPVDIEAHAQLVEPLRTLEPLVMSQSSSQLVEVPQNSLQQAEVQQSYATATTHSGTMQPTDAKINPDLAQKETKQEEVAAGVESEDKKFLESIFARMRKEETFSGQVKLMEWILQINNVTILGWFLTMGGLPIVSTWLNQAAMEEQTTVILIIFKVLLHLPLHKALPAHMSALLQTMNRLRFYRTQDISSRARNLLSRLSKVLVRSQASKKPQKDLICKQRISEILRDESWRSEVDITDEILALTEGASESRKPEPRKTPLLLTASADEPYKKSSVQTKSKERRKVLLVEHPNQKATGNNVHSVRSISTNSSRPLSADDIQKAKMRAMFMQEKYGKRDTGKGTDKTEMAEIKKPSGLVNSDVLPMPRSPPVSTTKQPVDPSPSTSKHNTVPLPDKPEIPVSPKPNITSRENSREKLDSKRVLWQIPPEVWIDPSWTLSAGENSKELDVQTQRNRREKETFYASLNDIPLNPKDPWDLEMDFDDSLTPEIPTDQPSDADTMEVDDVGTAPPSICFPDENNHVGSTSSSTVAAGANGAASEPDLELLAVLLKNPQLVFALSSNQVGNLPTEQTVALLDMLKKTGLGLPELVNSLSNGTGVPKASEPGPETIPTSLPSPTPPEDFPASASWRSEFPTQVRAPNLQQAHLPSRGNTSLVASTMHQSFSNVVSPLPSQPYTSISALPAHIQTNIPSSPQLAVSVNTLNQHVAPVNDLLNGAAVHRHTQSYALASDAAAGGIQQHPAVNKPAHEFQNISNPALAPAWQSSAANLASTGRNTTPEPWASRTTNSFNDASMPYLNQSAYSNQSTQSPYNSYASMSVSSQGLDRKGYTRTAEYQVSGRGTHQRHSLSPEPGAARVFGGAQAYVPEPLDVGNYGRQNYNPEPSRDWRSGQQSYAAAEPSSQWSSIQQSYPPAEPSRQWSSGQQSYVPADPSSQWSSGQQDYNQSDISRQWSSALQTYARAEPSRPSWNTANQVQVPDTSRQWSLGNQDYYNPNDSRSSYDQRQRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S7S2</accession>
    <name>A0A0Q3S7S2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g58849v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21114.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3S7S2"/>
    <dbReference type="EnsemblPlants" id="KQK21114">
        <property type="protein sequence ID" value="KQK21114"/>
        <property type="gene ID" value="BRADI_1g58849v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21114">
        <property type="protein sequence ID" value="KQK21114"/>
        <property type="gene ID" value="BRADI_1g58849v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3S7S2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK21114.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="137" mass="14329" checksum="12A83E90293DF044" modified="2018-03-28" version="2">MEKLVNQLQQLLDLPLMEIALAHTVFTAKFPSPPILQKKNSRLSPPRPRLPPSPPLAPSARVGASTAAPAPPSLRPRSGEELLAALPISPGSATSASHGPGSAAGALLVAPLSMAPYLFGCCTRWTTLRLVVSLNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CF82</accession>
    <name>A0A2K2CF82_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g03261v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CF82"/>
    <dbReference type="EnsemblPlants" id="PNT60687">
        <property type="protein sequence ID" value="PNT60687"/>
        <property type="gene ID" value="BRADI_5g03261v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT60688">
        <property type="protein sequence ID" value="PNT60688"/>
        <property type="gene ID" value="BRADI_5g03261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60687">
        <property type="protein sequence ID" value="PNT60687"/>
        <property type="gene ID" value="BRADI_5g03261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60688">
        <property type="protein sequence ID" value="PNT60688"/>
        <property type="gene ID" value="BRADI_5g03261v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CF82"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT60687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="153" mass="16650" checksum="7DFC8E7285F25358" modified="2018-03-28" version="1">MSAAAGLQLLRFYSTLRRRRTGTADQADMSRCPWYITGRAATASTPKKSASQQPRVRCAAVRVKEVAKWAASASPASPAPRRPAACQRRARRHQRAGARPARCIEKASLNGQDFLKKRKRSPASASIDAHSHREGGSVRSMSGTTGGKHRRPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHF0</accession>
    <name>A0A2K2CHF0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g15491v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHF0"/>
    <dbReference type="EnsemblPlants" id="PNT61461">
        <property type="protein sequence ID" value="PNT61461"/>
        <property type="gene ID" value="BRADI_5g15491v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61462">
        <property type="protein sequence ID" value="PNT61462"/>
        <property type="gene ID" value="BRADI_5g15491v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61461">
        <property type="protein sequence ID" value="PNT61461"/>
        <property type="gene ID" value="BRADI_5g15491v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61462">
        <property type="protein sequence ID" value="PNT61462"/>
        <property type="gene ID" value="BRADI_5g15491v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CHF0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT61462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="155" mass="16568" checksum="9AEF7C7B9564267B" modified="2018-03-28" version="1">MPDLQPPQRSPSSGTARAPRSRHAPQEARRGCRCCSRHAPPSSGAQPRSSPRPPSPSEQRAASVLVVAAAALRPTRQRRPPAPPQPSSSAPHRPPHSSPSWSAAISSVQHAVTIRRHPPRITSSQRRFSSRSTPSLLAAFAALPSNRALLIFLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CIR0</accession>
    <name>A0A2K2CIR0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BRADI_5g23050v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CIR0"/>
    <dbReference type="EnsemblPlants" id="PNT61924">
        <property type="protein sequence ID" value="PNT61924"/>
        <property type="gene ID" value="BRADI_5g23050v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61924">
        <property type="protein sequence ID" value="PNT61924"/>
        <property type="gene ID" value="BRADI_5g23050v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CIR0"/>
    <dbReference type="OrthoDB" id="608145at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CIR0">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000014">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000402">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1045">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033311391" description="Receptor-like serine/threonine-protein kinase" evidence="17">
        <location>
            <begin position="20"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="19">
        <location>
            <begin position="20"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="20">
        <location>
            <begin position="271"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="390"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="PNT61924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="698" mass="77509" checksum="535C7FCB49A778E7" modified="2018-03-28" version="1">MTICIPILILLLLSPSCKSDDTLTHAKPLFYGEKLVSKGGDFALGFFSPASSNKSFYLGIWYNNLPAAGRRTVVWVTNRDKPIAPPSSAALAVTNSSDLVLSDSQGVTYWKTKNNITAGRSTQLVAALLLDEGNFVIRMPNNTIIWQSFDHPTDIILPKMSSSVVSQSSVDPPDELYFTFTVSDGSPFTLIKLDHTGKITSLSWNNQSLSWGVISSRPDPAVPCDIYSSCGPFGYCDLTTRGPMCACLDGFEPNGVNISSGGCRRKEALECSKEAHFVTLPRMKVPDKFLHIRNRSLDECKAECTGNCACTAYAYANFSRNLAKADPSRCLVWTGELIDTEKYNGDSFFTAGKSINSGEDLYLRLADSHGKNIEFPFVNFESIVAATNNFSDSNLIGRGGFGKVYKGMLEGGNEVAIKRLGEGSRQGIEEFKNEIVLFAKLQHRNLVRLLGCCIHRDERLLIYEYLPNKSLDAFLFDSKRQYVLDWPTRFKIVKGIARGLLYLHQDSRLTIIHRDLKTSNILLDREMNPKISDFGMARIFGENQQQANTTRVVGTYGYMSPEYVMGGSFSVKSDTYSFGVLLLEIISGSKISSPHLTTNFSSLTAYAWKLWEDGQARELVDCLIVESCPLREVLRCVHVGLLCVQDHPNDRPLMSSVIFILENENALLPAPKQPLYFSTSQNMENSDNAMSITTQEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CK08</accession>
    <name>A0A2K2CK08_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g02324v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CK08"/>
    <dbReference type="EnsemblPlants" id="PNT62360">
        <property type="protein sequence ID" value="PNT62360"/>
        <property type="gene ID" value="BRADI_4g02324v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62360">
        <property type="protein sequence ID" value="PNT62360"/>
        <property type="gene ID" value="BRADI_4g02324v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CK08"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT62360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="396" mass="41987" checksum="1B1B364868FECDC0" modified="2018-03-28" version="1">MEEDMSYFVVYAWMENPDEIPKEVTLDIPEPPALGLPGQLADGLIDVFDDYLDSDNPNPPELLSHTVLIHLDSLLILPPHQGNSSRRGRRSDRVQAYRFCWARRRLDGTADIVEEMQPSVFMRLGCSGDLGCHREVSAQLASTRAPSPSVGESFRSSDAALLPPPYVPVMLPLPEASAQADLPPVVPAPSPAPAPPLAPAAPAPSPILVPVVLLPEPTALVDPPAPVVRAAAPPPTPVVVPHMVPHESAAPPLAPAVVADVAATAATLAGSPSLADFLHRVAEAVTPGLLQLARPAAGPVPPRKSKKPSPKPVRRSCRLASKKLPAFDDVLSRVKRLICKKLGVIFDEAASDDAAMLSRYAASFEKPLSEVQIAALTALAQRGAEKTKKAWVPAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CKW6</accession>
    <name>A0A2K2CKW6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g06746v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CKW6"/>
    <dbReference type="STRING" id="15368.A0A2K2CKW6"/>
    <dbReference type="EnsemblPlants" id="PNT62670">
        <property type="protein sequence ID" value="PNT62670"/>
        <property type="gene ID" value="BRADI_4g06746v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62670">
        <property type="protein sequence ID" value="PNT62670"/>
        <property type="gene ID" value="BRADI_4g06746v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CKW6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011676">
        <property type="entry name" value="DUF1618"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074:SF57">
        <property type="entry name" value="DUF1618 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33074">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07762">
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    </dbReference>
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        <location>
            <begin position="209"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT62670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="312" mass="33452" checksum="1F25D4AEF4E79824" modified="2018-03-28" version="1">MAATDGLYRRPPHAHDGSHDHDADHLPSSVILYNMVYLDARTNASTAGAVTSKGVAFMLTLWAAHPPRISYFTVRFPGTTSSALPDGNPRVLRTDGDLVLLRFLVVGPEGRSSPASTSFGSSPSTPSACSPTWLSASSAAAATPTTTKKGSSSLRSVGLPVGTTPSTCSTQCHGHGALGPCMWIVESPEGFCFTVPDKVINLHGSSIGWVDIWNGILLFDSLRGDDNDTLQFIRSPRPPPVPNKLARRAVACTRDVSFSDDGCLRFTDVWAHAVPGSDDGLNYISQDWGAAVMKWFGPEKKWQVDRLHAQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CLS5</accession>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BRADI_4g10107v3</name>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CLS5"/>
    <dbReference type="EnsemblPlants" id="PNT62975">
        <property type="protein sequence ID" value="PNT62975"/>
        <property type="gene ID" value="BRADI_4g10107v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62975">
        <property type="protein sequence ID" value="PNT62975"/>
        <property type="gene ID" value="BRADI_4g10107v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CLS5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT62975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="78" mass="9009" checksum="737D86334CDC182C" modified="2018-03-28" version="1">MPLISGRMDFFFYLMDLLVASIILSCVVMHLHYLYSTMAMLVDIYAGSRCALLLFYPGRRLTIAIWSLGGSYYYEHVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CM02</accession>
    <name>A0A2K2CM02_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Knottin scorpion toxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC104584454</name>
        <name evidence="2" type="ORF">BRADI_4g10932v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010239150.2"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT63049">
        <property type="protein sequence ID" value="PNT63049"/>
        <property type="gene ID" value="BRADI_4g10932v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584454"/>
    <dbReference type="Gramene" id="PNT63049">
        <property type="protein sequence ID" value="PNT63049"/>
        <property type="gene ID" value="BRADI_4g10932v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584454"/>
    <dbReference type="OrthoDB" id="667443at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043158505" description="Knottin scorpion toxin-like domain-containing protein" evidence="1">
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            <begin position="30"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11599" checksum="B52205CE9A98F9EB" modified="2018-03-28" version="1">MADTRRAAALCVLLPLLVLSLANPYPVEAGCRYTYPWMPFCKGWMCKAECWAEAKLDFASLKEHYCVKGGFKGYCYCMFCSKHLRDHDDEVQGEKPKELLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CPI0</accession>
    <name>A0A2K2CPI0_BRADI</name>
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        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g22564v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CPI0"/>
    <dbReference type="EnsemblPlants" id="PNT63947">
        <property type="protein sequence ID" value="PNT63947"/>
        <property type="gene ID" value="BRADI_4g22564v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63947">
        <property type="protein sequence ID" value="PNT63947"/>
        <property type="gene ID" value="BRADI_4g22564v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CPI0"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Small kernel1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF516">
        <property type="entry name" value="SMK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="121"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="261"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT63947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="50810" checksum="194B8F830B4549DE" modified="2018-03-28" version="1">MPPPTAGRLSGLLRRCATTGALPAGTLLHAQVIVGGHLPQATLETDLVFDAMPSPSMHADNILLAASPPRAALELLSGLIAAGFRPDRYAVPAALRACAELQDPLLGAALHGFAVHFGFLNIVVSCALLDMYAKSGLLANAARVFDEMPEKDSVVWNCMVTAYAAETLELFRRAQVEVVNMARDLRSVPSVLNACGKEGELMKGREIHEIHGRMVRCLAFDSDVPIGNASIDMYAKCGHVDSGISPTDSSQAVFAGMLDRNVVSWSTLIACYGVHGKGKEAVRIYEEMLPQRFKPNGITFMSVVSSCSHSGLVTDGRMIFESMSKVHGVEPTAEHYACVVDILGRAGAIEEAVRLIRKMPMGPCASSWGAVLSACATYNNLEEDNASNYVTLCGIYDAVGQLDGVAGLRSRMRELGMVKTPGCSWVDMKGRAHAFYQGSIPRYLRRPMVWISRIWEVQNLNMSMRTSITC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CSX4</accession>
    <name>A0A2K2CSX4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="4">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100842391</name>
        <name evidence="7" type="ORF">BRADI_4g37862v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="4"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578641.1">
        <property type="nucleotide sequence ID" value="XM_003578593.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CSX4"/>
    <dbReference type="STRING" id="15368.A0A2K2CSX4"/>
    <dbReference type="EnsemblPlants" id="PNT65135">
        <property type="protein sequence ID" value="PNT65135"/>
        <property type="gene ID" value="BRADI_4g37862v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842391"/>
    <dbReference type="Gramene" id="PNT65135">
        <property type="protein sequence ID" value="PNT65135"/>
        <property type="gene ID" value="BRADI_4g37862v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842391"/>
    <dbReference type="OrthoDB" id="429813at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010345">
        <property type="term" value="P:suberin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF75">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="387"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="6">
        <location>
            <begin position="17"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="5">
        <location>
            <begin position="393"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT65135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="496" mass="55762" checksum="A0C390D704E76767" modified="2018-03-28" version="1">MDATAVAGCFRDKIILVTGSTGFLGKLLVEKILRVQSDVKKLYLLVCAPDAASAEQRILSQVLGKDLFNTLREKHGLAGFQKLFKEKIVPLAGDIGDRNFGLNSSRADALCKEIDVIVNGAATTSFYERYDVSLASNALGAKYACEFAKKCTNLKLLLHVSTAFVAGTQGRLLEKTLQMGETLRQGYYLDIEAELQLAEKVKTELKTSKSGSSDQLEKTAMKELGLKRACHFGWPNVYTFTKAMGEMLLAEQWGDLPVVIIRPNMVTSTYQDPFPGWIEGARTIDALIVAYDEQAFPCFVGDRKDIMDVVPADMVVNATLVAMVVHWNQKGKVVYHVSSGLQNPLTGYVLEDACLDYFSIHPRVLENGKTLQNRRPYLFKRFAYFRAYLILVYKLPLEILHAVSLLSCGLFSKYYNKHNRRYGFLMLLVKLYTPYAFFEGCFDDTNLTRLRKEVKMDGSDGSIFNFDPKSMDWHTYLLNVHVPAVLKYGRKKKGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CTP5</accession>
    <name>A0A2K2CTP5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g41641v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CTP5"/>
    <dbReference type="EnsemblPlants" id="PNT65393">
        <property type="protein sequence ID" value="PNT65393"/>
        <property type="gene ID" value="BRADI_4g41641v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65393">
        <property type="protein sequence ID" value="PNT65393"/>
        <property type="gene ID" value="BRADI_4g41641v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CTP5"/>
    <dbReference type="OrthoDB" id="687490at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34591:SF32">
        <property type="entry name" value="OS03G0653100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34591">
        <property type="entry name" value="OS03G0653100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="4"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT65393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="314" mass="36072" checksum="8C18789EF115DA5C" modified="2018-03-28" version="1">MDVLADDLAADVLGRLDPCNLAACRCVCKSWRAAVDAHRLLRKDLLPLSLAGIFADYSSRVRRRRTELDPANPGSEWPPSPFVMSVFSSRTWRWEERSFVKDGMALRSITSVAPFLERVSSRHDAVYWRGHLYVCHIYFIMRINLEDSKYQVWFLDESTHGTHEWTLKHDSDLDLEPLLGNFPWKHGDGPWSVQGHSVNAEATLEKEEKPEWDSDKDDGISIATDADDIIWKGFSISILGFHPYKEIVFLYTSSDRVMAYHLNSSKAEDLISLPLDLFDYDMQMQLGLSFTYTPCWMEINCLRETSVDRALGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CY10</accession>
    <name>A0A2K2CY10_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g18575v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CY10"/>
    <dbReference type="STRING" id="15368.A0A2K2CY10"/>
    <dbReference type="EnsemblPlants" id="PNT66919">
        <property type="protein sequence ID" value="PNT66919"/>
        <property type="gene ID" value="BRADI_3g18575v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66919">
        <property type="protein sequence ID" value="PNT66919"/>
        <property type="gene ID" value="BRADI_3g18575v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CY10"/>
    <dbReference type="OrthoDB" id="602113at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47851:SF5">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47851">
        <property type="entry name" value="OS06G0588700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="276" mass="31628" checksum="14EEC5F67E203CAB" modified="2018-03-28" version="1">MAAVNWNDDNTRIITELFADQVHRGNRPNSHLNNVTGIELKKKQIKNKWDKLKNEYGIWKKLLLKQTGASWERGTINQDAEWWRKAKVMDLFQLDIPGCGRFQNQGIRNEDNLELIFEDITNDGHDHWCPTSGDLPQPNIVEDVINLDGEDGIDIDELDESPSNVKGKKRGAKVVGDKSKKSKTSQVMQDEMKKIGALAEKTQSSLESFTTKNDACSVATVMDLVIECGGAYGTNEHFIATELFVKREQREMFLHMPTPDIRFEWLKRKYEAKYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CZA2</accession>
    <name>A0A2K2CZA2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g26040v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CZA2"/>
    <dbReference type="EnsemblPlants" id="PNT67357">
        <property type="protein sequence ID" value="PNT67357"/>
        <property type="gene ID" value="BRADI_3g26040v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67357">
        <property type="protein sequence ID" value="PNT67357"/>
        <property type="gene ID" value="BRADI_3g26040v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CZA2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT67357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="136" mass="15733" checksum="B4A8E6C4168D29CF" modified="2018-03-28" version="1">MIQSDTHSYSSSSSCRVGGHYRWRDMYNFPLYIYHQPNSEICTIFHRSPPSNFCRDQTLKRPKRISPGVSTTKKDSWCYKIQRLLLAAGVTLKHIRCGFIYGSSSHKYTHALCRNCSVSSFTANYLSIYMNVYPIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D1X6</accession>
    <name>A0A2K2D1X6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g38179v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D1X6"/>
    <dbReference type="EnsemblPlants" id="PNT68280">
        <property type="protein sequence ID" value="PNT68280"/>
        <property type="gene ID" value="BRADI_3g38179v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68280">
        <property type="protein sequence ID" value="PNT68280"/>
        <property type="gene ID" value="BRADI_3g38179v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D1X6"/>
    <dbReference type="OrthoDB" id="667197at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF5">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 8-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="6">
        <location>
            <begin position="562"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="677"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="775"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="677"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="793"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT68280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="829" mass="95711" checksum="BC006CC656AB3AB7" modified="2018-03-28" version="1">MLSEEDIDIFLDNEVITEAVTSSQEVRDSLVPRMGMLFDSDKEAYEFYNTYASVSGSSAKKASCYHSRKRDIGKPTRHTFKCNRCGKVLDKEKQGEKRRDEQIRRAEKKVIQGLQPQKKRKRNLLEVTDCKAQMVISLRSGKWEVITLEVEHNHELSPPDETRFLISHKHMTNEEKQLIRTLNAVKLPTRKIMGILAVYRGGIKAVPYNKKYVSNYMTAIKQESRTNDMMQVSEYFTKRESLDPLFYFAIKLDVNNTVKSLFWADSNARRLYEAYGDCVSFDTTYGTNRYNLPFSPFVGITGHGSNCLFGCSILENETIETFKWLFETFLHRMHGKHPVSIITDQDVAMKQAIPLVFKNVTHRNCLWHIKKKAEERCAKAFATKRNLHEEFNDILNNTLTKEEFEELGPQMIVKYGVEDIKYLQDMWLDRRRFVPVYYKNVFFPFINSTTRSEGTNAIFKDNVCSTYTVISLLGEYQTIAENIEEKEKELDLVTRTTNPSYIHCSEMEIVTGKAYNRAIFYKFQKQLRFTSNLHVDEVQRFEKYEVYKSNYYAAMEFRSRRYVVAVDMKNKDFACLCCKFQKDGILCAHILKVLIHLNIPRIPDKYIIQRWRPRDRKVERDKRYNIPRELTCGNSQLRYNILCRWYTDISSEGAQHNEDYLMLVEEAKRLQEKLHANAAAREGRKGSSDEGSGQGNPNAAEPSAVPVQPKPHIQINDPTVATAKGRPRGTGRMKSVVERIFTKQRITCSHCGSHDHNIATCTMLHLDRRCAQGTKKQTRKVQKKGGGGKPTGNIETSRNEGSTSVVTVEAAEHILRRMDLEAAKVARAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D229</accession>
    <name>A0A2K2D229_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106866456</name>
        <name evidence="2" type="ORF">BRADI_3g38831v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="2">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="3">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_3g50632v3"/>
    </dbReference>
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            <begin position="6"/>
            <end position="22"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT69170.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69170"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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    </evidence>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
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            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <property type="protein sequence ID" value="PNT69202"/>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT69202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">Bd21</strain>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
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        <reaction evidence="1 12">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        <text evidence="2">Protein modification; protein ubiquitination.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051348">
        <property type="entry name" value="U-box_ubiquitin_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647">
        <property type="entry name" value="OS02G0152300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647:SF94">
        <property type="entry name" value="OS02G0822900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="480"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="8">
        <location>
            <begin position="772"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="193"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="288"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="339"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT69465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="857" mass="92222" checksum="61F6F4BBA79EAEFC" modified="2018-03-28" version="1">MEVGGGSHEEEEEEEVPAAVVYCAVGKDGGREWKANLRWVLANFPHRSRRRFSLVLAHVHRPPHRVNMMGAWVPVSQLAEHEVAAYSKLEEDRASRALDDLLHICTSQRVRARKVIVSADDAARGLVRLADDHAVAELVMGAASDRAYTRKMCAPRSKKAVTVQRKANPACRIWFLCKGNLICTREADEVGLNRAEPSTSSSSPSPRSSSAVSDCSRSKPSMPFGMHESASAGPTTLRRRTSSRDADSDNATDHNGAEDDHLGHDASSSSAAAAADVVGAFFSTAPAASGLRDAEDQDSPAPSHHGSSDGAGEMDDDALYERLKEALVEARNLRHEAYEETRRRQKADRDLAHASRMAKEAESSWQGEARRRKETEERLARERVAMEQDRRDLDGILEKIMEVDGRSAELELQIADSERAMSQLDVRLSESYGVLDALRLECERREEPATAGEPSVPEVDVAEQSMSFWRLGLSELEEATGHFNESARIGGGGVYRGSLRGMSVAVRMVSPEVAVDEARFTRAVEAMSRARHPGLVTFLGACPEARAVVHELVPGGSLEDRLEGKEAPTLSWQARCGVAYRTCSALAYILSTGAVHGDVRPANILLEDEGCSSSKLAGFGTRGLVAAKERRRPGGVDVAYVDPRCLATGEPTPRSDVHALGVVLLRLVTGKPAFEARKAARDAAAGSTPWHEAVGHAGAGGWPVERATEVALLGLKCCAGDEDEDVAGGGATAEQLLEEARGVLEAATMAAPGRTWSSLSSASGAGSESGGGAPSYFLCPILKVEVMRDPQIAGDGFTYEAEAMREWLGSGHDTSPMTNLKLPTDELLPNHALRAAIQEWRHTTTPGTSSASSDRFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D971</accession>
    <name>A0A2K2D971_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100831540</name>
        <name evidence="5" type="ORF">BRADI_2g18550v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D971"/>
    <dbReference type="STRING" id="15368.A0A2K2D971"/>
    <dbReference type="EnsemblPlants" id="PNT70813">
        <property type="protein sequence ID" value="PNT70813"/>
        <property type="gene ID" value="BRADI_2g18550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70813">
        <property type="protein sequence ID" value="PNT70813"/>
        <property type="gene ID" value="BRADI_2g18550v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D971">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000023">
        <property type="entry name" value="Aldehyde dehydrogenase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000019">
        <property type="entry name" value="probable aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521:SF7">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 12A1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="77"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT70813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="562" mass="62277" checksum="EFC471FFA0CC7555" modified="2018-03-28" version="1">MLGRRRVIIWCEFVTRMLESLSRRLRFEGRSGLVAFRWLHTPAFATVSPEEISGSSPAEVQNFVQGKWIASANSSWIVDPLNGDKFIKVAEIQGSEIKPFVESLSNCPKHGLHNPLRAPERYLMYGDISAKAAHVLGQPEVLDFFAKLVQRVSPKSYQQALAEVQVSQKFLENFCGDQVRFLARSFAVPGNHLGQISNGYRWPYGPVAIITPFNFPLEIPLLQLMGALYMGNKPVLKVDSKVSIVMEQMIRLLHECGLPAEDVDFINSDGITMNKLLLEANPKMTLFTGSSRVAEKLAADLQGRIKLEDAGFDWKILGPDVQEVDYISWVCDQDAYACSGQKCSAQSMLFMHKNWSSSGLLEKMKGLSERRRLEDLTIGPVLTVTTASMIEHMNNLLNIPGSKVLFGGEPLENHSIPVIYGAFKPTAVFVPLTEILKSGNFELVTKEIFGPFQVVTEYSEDQLELVLEACERMNAHLTAAVVSNDPLFLQEVLGRSVNGTTYAGIRARTTGAPQNHWFGPAGDPRGAGIGTPEAIKLVWSCHREVIYDIGPVPKNWALPSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DER7</accession>
    <name>A0A2K2DER7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g49168v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DER7"/>
    <dbReference type="EnsemblPlants" id="PNT72791">
        <property type="protein sequence ID" value="PNT72791"/>
        <property type="gene ID" value="BRADI_2g49168v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72791">
        <property type="protein sequence ID" value="PNT72791"/>
        <property type="gene ID" value="BRADI_2g49168v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DER7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT72791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="110" mass="11793" checksum="6D5B83F4EFA25DF3" modified="2018-03-28" version="1">MRVHPKFHASSSSSTSSDSSPREDPQIPDSLFPVRTRGTGSNPHLLRAAAAWGHGRGRAAPDRRREREMGKIEYVVVARGAVVLVEHNGAGVATNAGAVARQTLERTLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DJG1</accession>
    <name>A0A2K2DJG1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g14455v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DJG1"/>
    <dbReference type="EnsemblPlants" id="PNT74414">
        <property type="protein sequence ID" value="PNT74414"/>
        <property type="gene ID" value="BRADI_1g14455v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74414">
        <property type="protein sequence ID" value="PNT74414"/>
        <property type="gene ID" value="BRADI_1g14455v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DJG1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT74414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="32" mass="3584" checksum="003E2C8A49B0B284" modified="2018-03-28" version="1">MIYLFLAGKFESCENWRSASPTVFSALPVSMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKL3</accession>
    <name>A0A2K2DKL3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g22563v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DKL3"/>
    <dbReference type="EnsemblPlants" id="PNT74818">
        <property type="protein sequence ID" value="PNT74818"/>
        <property type="gene ID" value="BRADI_1g22563v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74818">
        <property type="protein sequence ID" value="PNT74818"/>
        <property type="gene ID" value="BRADI_1g22563v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DKL3"/>
    <dbReference type="OrthoDB" id="583300at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165:SF87">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT74818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="32984" checksum="0DA8493D74A8DD8F" modified="2018-03-28" version="1">MNMRTTTKLGRSLADPFSYCDGVNNNLIEEITSRLLALNIVNYTHFRATCKHWRDCTDDPRVCGVMDSRFRSRDWVEPSLSCSQMLPTFLNTNTLTCVVVDFPAELTTHRHLGTADGLIIIGHNAANIIYILNPLTRLVVKFPTITIEQAMKVEMGMLHVDQSDIIGTGIDDSTSTPTLVLRLRHESCQVICAKPGDRHSTTQNISSTLARSKDRMLMIRPKEDGYNETQMFLLHQPPSCLRPSCVEMFEVDVEGRRLVQWRNGVSFIREHNNVKVFTKKWHIDILHCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKR1</accession>
    <name>A0A2K2DKR1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC104583969</name>
        <name evidence="8" type="ORF">BRADI_1g23294v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236324.1">
        <property type="nucleotide sequence ID" value="XM_010238022.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DKR1"/>
    <dbReference type="STRING" id="15368.A0A2K2DKR1"/>
    <dbReference type="EnsemblPlants" id="PNT74862">
        <property type="protein sequence ID" value="PNT74862"/>
        <property type="gene ID" value="BRADI_1g23294v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74863">
        <property type="protein sequence ID" value="PNT74863"/>
        <property type="gene ID" value="BRADI_1g23294v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74864">
        <property type="protein sequence ID" value="PNT74864"/>
        <property type="gene ID" value="BRADI_1g23294v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583969"/>
    <dbReference type="Gramene" id="PNT74862">
        <property type="protein sequence ID" value="PNT74862"/>
        <property type="gene ID" value="BRADI_1g23294v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74863">
        <property type="protein sequence ID" value="PNT74863"/>
        <property type="gene ID" value="BRADI_1g23294v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74864">
        <property type="protein sequence ID" value="PNT74864"/>
        <property type="gene ID" value="BRADI_1g23294v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583969"/>
    <dbReference type="OrthoDB" id="681759at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014294236" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="6">
        <location>
            <begin position="27"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="93"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="231"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="252"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT74863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="268" mass="27267" checksum="C0C30F2341B78E9B" modified="2018-03-28" version="1">MALSKHLVALFFAFAVVAAALQPSDARPQGFEEEADQSKAATTANGGAPPGLPSFPGLPLPQIPGFTLPLPQIPGFPNLPPLFRFPLPPLFGPRPGAPGAPPSRPLPNLPHFPSIPGSPGAPPFHLPGLPTTPVPPPPAECLTPLTGMALCMDYLTNLTVTTPPSGCCDGLKSVITKAPICLCHGMNGGMSKLAPKPIDPIRMLILPARCGTMIPIQTLFSCATTHLPPLTPPTSPAAPTPPTSPATPAPPVVSSGSPAPASSSVSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DT81</accession>
    <name>A0A2K2DT81_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g63616v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DT81"/>
    <dbReference type="EnsemblPlants" id="PNT77487">
        <property type="protein sequence ID" value="PNT77487"/>
        <property type="gene ID" value="BRADI_1g63616v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77487">
        <property type="protein sequence ID" value="PNT77487"/>
        <property type="gene ID" value="BRADI_1g63616v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DT81"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT77487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="211" mass="23208" checksum="84F7C60F4E6228DD" modified="2018-03-28" version="1">MTASKHKGSTEPQTCNLPHTSILTSLHFSKLNTCVVKLALHEQGFCRTNAPGSSLTGAGKDPTKKSSDLLGKQLGHQQEREKSWIFRISGDESMGILGKRRRKGGTGLTGPPCRSPATLNRSNGEDVTGQSRLGPRETTSKEMKRRQLAKIYKAHTLLPLPLRPAAPAEERGRGKSPPTGTTKKTRGRWRVRERKSGRSVSADEMALYRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DTE6</accession>
    <name>A0A2K2DTE6_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100839028</name>
        <name evidence="2" type="ORF">BRADI_1g64698v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DTE6"/>
    <dbReference type="EnsemblPlants" id="PNT77548">
        <property type="protein sequence ID" value="PNT77548"/>
        <property type="gene ID" value="BRADI_1g64698v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77548">
        <property type="protein sequence ID" value="PNT77548"/>
        <property type="gene ID" value="BRADI_1g64698v3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT77548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="179" mass="19172" checksum="1D28BC514BBB08EF" modified="2018-03-28" version="1">MSLRNTQRREPRANEDDEEEEVEEDEDERDEAVTGGGASSDGAPVERDTEARSGGGTSALSSLSRGDVLRIGDMDRARRCTEPLRSVSSRPRAGPCRRSSSSLSSSLPGSRSGSGDRVSGRIWPFWKSSSAEIMCSPGPPNSNSASPMPPYPAPFAAPEAADEDENSRWWMGLAGALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DU56</accession>
    <name>A0A2K2DU56_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_1g69063v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DU56"/>
    <dbReference type="EnsemblPlants" id="PNT77802">
        <property type="protein sequence ID" value="PNT77802"/>
        <property type="gene ID" value="BRADI_1g69063v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77802">
        <property type="protein sequence ID" value="PNT77802"/>
        <property type="gene ID" value="BRADI_1g69063v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DU56"/>
    <dbReference type="OrthoDB" id="786283at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF19">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="6">
        <location>
            <begin position="3"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT77802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="215" mass="23839" checksum="852B038DF1417CBA" modified="2018-03-28" version="1" fragment="single">GLNNPSRRAAVRDLIHDTHTTIICIQETKLQVVDDRLIRDLLGPCFSANFAVLPAAGTRGGMILAVSEDFFTISDVPLSAHSITVTVTMRSEGALWSLTSVYGPQGDQEKLLFIEELKLLQPVVKSEWILLGDFNLIAKAADKNNTNINRRLIGKFRENPVLAKIDHVFCSDDWDMMFPNALLLAVPTLCSDHAPLFLQGATPSCHKSSFKFEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DV72</accession>
    <name>A0A2K2DV72_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peroxidase</fullName>
            <ecNumber evidence="3">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100834380</name>
        <name evidence="22" type="ORF">BRADI_1g74882v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
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        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
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        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_003562023.4"/>
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        <property type="gene ID" value="BRADI_1g74882v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834380"/>
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        <property type="gene ID" value="BRADI_1g74882v3"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="entry name" value="Haem_peroxidase_sf"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
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        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 16" id="KW-0408">Iron</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="21">
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            <begin position="78"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
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            <begin position="391"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
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            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
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            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
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            <begin position="432"/>
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        </location>
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    <feature type="active site" description="Proton acceptor" evidence="14">
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        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
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        <location>
            <position position="125"/>
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        <ligand>
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        <ligand>
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        <location>
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        <location>
            <position position="215"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="246"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
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        <location>
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        <ligand>
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        <ligand>
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        <ligand>
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    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50873"/>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="447" mass="47310" checksum="B52C86EF3591771E" modified="2018-03-28" version="1">MRALAAPRGDRGSPVDRPGIKRGPRPGPGSPYCIARRRELDPFARTYTYEISHQMDARRLLLVFGLLAAAAPLASAGDLSLDFYKSSCPDAEKIVTATIEKKIKEEPGTAAGLLRLLFHDCFANGCDASILIDPLSNQSAEKEAGPNISVRGFEIIDEIKKELESKCPNTVSCADIVALSARDAVKLAGGPSYDLPTGRRDSLVSNREEADNNLPGPDIPVPKLIMDFVDKGFTAEEMVALLAGGHSIGQVRCIFIEPDATPMEPGYHAAISKLCDGPNRDTGMVKMDETTPNVVDGGSYFDLVLAKKMPLTIDRLLGLDSKTMPIIKEMSSKPDQFVPLFAKSMEKLSALKVITGKDGEIRKTCSEFNNPVAADSGASVIRISSVDPEDLEGLSAGGTHVSDGVSHGTLEPEKGAGSGGAAPAGKGAGEGKKHHKGKGHLKLRGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-09-02" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>B3TN63</accession>
    <accession>A0A6N2NMU2</accession>
    <name>B3TN63_BRADI</name>
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        <recommendedName>
            <fullName evidence="4 18">Cytochrome f</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18 21 25" type="primary">petA</name>
        <name evidence="24" type="ORF">BDC2019_01_057</name>
        <name evidence="22" type="ORF">BN3904_07_057</name>
        <name evidence="23" type="ORF">BRADI_1g39211v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <geneLocation type="chloroplast" evidence="21"/>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2008" name="BMC Res. Notes" volume="1" first="61" last="61">
            <title>The complete chloroplast genome sequence of Brachypodium distachyon: sequence comparison and phylogenetic analysis of eight grass plastomes.</title>
            <authorList>
                <person name="Bortiri E."/>
                <person name="Coleman-Derr D."/>
                <person name="Lazo G.R."/>
                <person name="Anderson O.D."/>
                <person name="Gu Y.Q."/>
            </authorList>
            <dbReference type="PubMed" id="18710514"/>
            <dbReference type="DOI" id="10.1186/1756-0500-1-61"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23 25" key="2">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">Bd21</strain>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="journal article" date="2017" name="New Phytol." volume="0" first="0" last="0">
            <title>Comparative plastome genomics and phylogenomics of Brachypodium: flowering time signatures, introgression and recombination in recently diverged ecotypes.</title>
            <authorList>
                <person name="Sancho R."/>
                <person name="Cantalapiedra C.P."/>
                <person name="Lopez-Alvarez D."/>
                <person name="Gordon S."/>
                <person name="Vogel J.P."/>
                <person name="Catalan P."/>
                <person name="Contreras-Moreira B."/>
            </authorList>
            <dbReference type="DOI" id="10.1111/nph.14926"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="23" key="4">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="5">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="6">
        <citation type="submission" date="2019-03" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Sancho R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1 18">Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="18 19">Binds 1 heme group covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17 18">The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Plastid</location>
            <location evidence="2 18">Chloroplast thylakoid membrane</location>
            <topology evidence="2 18">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cytochrome f family.</text>
    </comment>
    <dbReference type="EMBL" id="EU325680">
        <property type="protein sequence ID" value="ACF08652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="LT222229">
        <property type="protein sequence ID" value="CZT22496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="LR537449">
        <property type="protein sequence ID" value="VGO01601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_002000499.1">
        <property type="nucleotide sequence ID" value="NC_011032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B3TN63"/>
    <dbReference type="STRING" id="15368.B3TN63"/>
    <dbReference type="EnsemblPlants" id="KQK18177">
        <property type="protein sequence ID" value="KQK18177"/>
        <property type="gene ID" value="BRADI_1g39211v3"/>
    </dbReference>
    <dbReference type="GeneID" id="6439865"/>
    <dbReference type="Gramene" id="KQK18177">
        <property type="protein sequence ID" value="KQK18177"/>
        <property type="gene ID" value="BRADI_1g39211v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:6439865"/>
    <dbReference type="eggNOG" id="ENOG502QPT8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033498_0_0_1"/>
    <dbReference type="OMA" id="PFWAQQN"/>
    <dbReference type="OrthoDB" id="604292at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.700:FF:000001">
        <property type="entry name" value="Cytochrome f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000007">
        <property type="entry name" value="Cytochrome f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.830:FF:000001">
        <property type="entry name" value="Cytochrome f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.830">
        <property type="entry name" value="Cytochrome f large domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.700">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00610">
        <property type="entry name" value="Cytb6_f_cytF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024058">
        <property type="entry name" value="Cyt-f_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002325">
        <property type="entry name" value="Cyt_f"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024094">
        <property type="entry name" value="Cyt_f_lg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036826">
        <property type="entry name" value="Cyt_f_lg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33288">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33288:SF10">
        <property type="entry name" value="CYTOCHROME F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01333">
        <property type="entry name" value="Apocytochr_F_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16639">
        <property type="entry name" value="Apocytochr_F_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00610">
        <property type="entry name" value="CYTOCHROMEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103431">
        <property type="entry name" value="Cytochrome f subunit of the cytochrome b6f complex, transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49441">
        <property type="entry name" value="Cytochrome f, large domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51010">
        <property type="entry name" value="CYTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="21" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12 18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 18 19" id="KW-0349">Heme</keyword>
    <keyword evidence="14 18 19" id="KW-0408">Iron</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 18" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="8 21" id="KW-0934">Plastid</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 18" id="KW-0732">Signal</keyword>
    <keyword evidence="15 18" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome f large" evidence="20">
        <location>
            <begin position="36"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="18 19">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="18 19">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="18 19">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="18 19">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR602325-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ACF08652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CZT22496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KQK18177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="VGO01601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="320" mass="35383" checksum="FA83236D3ADC1E2F" modified="2008-09-02" version="1">MENRNTFSWVKEQMTRSISVSIMIYVITRTSISNAYPIFAQQGYENPREATGRIVCANCHLASKPVDIEVPQAVLPDTVFEAVLRIPYDMQLKQVLANGKKGGLNVGAVLILPEGFELAPPDRISPELKEKIGNLSFQSYRPDKKNILVIGPVPGKKYSEIVFPILSPDPATKKDAHFLKYPIYVGGNRGRGQIYPDGSKSNNTVYNATSTGIVKKILRKEKGGYEISIVDASDGRQLIDIIPPGPELLVSEGESIKLDQPLTSNPNVGGFGQGDAEIVLQDPLRVQGLLFFFASVILAQVFLVLKKKQFEKVQLYEMNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1GML5</accession>
    <name>I1GML5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Actin-related protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100841675</name>
        <name evidence="10" type="ORF">BRADI_1g06640v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential protein required during embryogenesis and all plant development stages, probably through a chromatin-mediated regulation of gene expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the actin family. Plant ARP7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558271.1">
        <property type="nucleotide sequence ID" value="XM_003558223.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GML5"/>
    <dbReference type="STRING" id="15368.I1GML5"/>
    <dbReference type="EnsemblPlants" id="KQK12888">
        <property type="protein sequence ID" value="KQK12888"/>
        <property type="gene ID" value="BRADI_1g06640v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841675"/>
    <dbReference type="Gramene" id="KQK12888">
        <property type="protein sequence ID" value="KQK12888"/>
        <property type="gene ID" value="BRADI_1g06640v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841675"/>
    <dbReference type="eggNOG" id="KOG0676">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_0_3_1"/>
    <dbReference type="OMA" id="YMPENML"/>
    <dbReference type="OrthoDB" id="74201at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10209">
        <property type="entry name" value="ASKHA_NBD_AtARP7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000150">
        <property type="entry name" value="Actin-related protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000026">
        <property type="entry name" value="Actin-related protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK12888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="360" mass="39636" checksum="32FDB29FC2727F78" modified="2012-06-13" version="1">MEAVVVDAGSKLLKAGIALPDQAPALVMPSKMKTEVEDSQLADGAVVEEVVQPVVRGFVKDWDAMEDLLHYVLYRNIGWEMGDEGQILFTEPLFTPKALREQLAQLMFEKFNVSGFYDSEQAVLSLYAVGRISGCTVDIGHGKIDIAPVCEGAVQHVASKRFEIGGTDLTNLFAQELKNSNPSVNIDISDVERLKEQYACCTEDQLAFEAIESSCDPEKHTLPDGQVITIEKERFIVGEALFQPRILGLEDYGVVHQLVSSVSNVASEYHKQLLENTMLCGGTASMTGFEDRFQREANLSASAIRPTLVKPPEYMPENLGRHSAWLGGAILAKVVFPQNQHVTKGDYDETGPSIVHKKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1GU35</accession>
    <name>I1GU35_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100841481</name>
        <name evidence="14" type="ORF">BRADI_1g26560v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Potassium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237860.1">
        <property type="nucleotide sequence ID" value="XM_010239558.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GU35"/>
    <dbReference type="FunCoup" id="I1GU35">
        <property type="interactions" value="434"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GU35"/>
    <dbReference type="EnsemblPlants" id="KQK16068">
        <property type="protein sequence ID" value="KQK16068"/>
        <property type="gene ID" value="BRADI_1g26560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841481"/>
    <dbReference type="Gramene" id="KQK16068">
        <property type="protein sequence ID" value="KQK16068"/>
        <property type="gene ID" value="BRADI_1g26560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841481"/>
    <dbReference type="eggNOG" id="ENOG502QPSA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008142_2_0_1"/>
    <dbReference type="OMA" id="IWHAVAN"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GU35">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00794">
        <property type="entry name" value="kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF8">
        <property type="entry name" value="POTASSIUM TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="555"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="12">
        <location>
            <begin position="106"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="13">
        <location>
            <begin position="607"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="707"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="712"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU321113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK16068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="850" mass="94201" checksum="68366CD73B9E144E" modified="2012-06-13" version="1">METGSGGGGRLPKSESAEMRWVVPGGADDEDEIESSDDGFGGTDTPVAASGSRGGCSDADEDEEDALLHHRLVRTGPRADSFDVEALDVPGLYRHQEFTLCRSIVLTLQTLGVVFGDVGTSPLYTFDIMFNKYPNTSKEDVLGALSLVIYTLILVPLLKYTLIVLWGNDNGEGGIFALYSLICRNAKASLLPNQLPSDTRISSFQLKVPSVELERSLRIKERLETSSMLKKLLLMLVLFGTSMVIADGVVTPAMSVMSAVNGLKVGISSVNEGEVVMITVAFLIVLFSLQRFGTSKVGLVVGPALFIWFCCLSGIGIYNIMTYGSEVFRAFNPIYMYYYFERKPTEAWMSLGGCLLCATGSEAMFADLCYFSVRSVQLTFVCLVLPCLLLGYLGQAAFLLENLTENEQVFFLSIPTQVFWPVVFIATLAALIASRTMTTAIFSIIKQATALGCFPRLKIIHTSRKFMGQIYIPVMNWFLLVSCLAFVTTFGSINEIGNAYGIAELGVMMMTTILVTIIMLLIWQVNIVVVLCFLTLFLGLELFFFSSVLGSVADGSWVLLVFTAALYLIMYIWNYGTKLKYETEVKQKLSMDLMMDLGCNLGTVRAPGIGLLYNELVRGVPAIFGHFLTTMPAIHSMIIFVCIKWVPVPVVPQNERFLFRRVCPKNYHMFRCIARYGYKDVRKENPQAFEQLLIESLEKFIRREAQERSLESDENGDTDSEEEVASSSSRVLVGPNGSIYSLGVPLLDESAGASNPTLGSSTSFDGSLDETMDGRRSLDNELSFIHKAKESGVVYLLGHGDIRARKESFFVKKLVINYFYAFLRKNCRRGIATLSIPHTRLMQVAMQYMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWG2</accession>
    <name>I1GWG2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100838538</name>
        <name evidence="2" type="ORF">BRADI_1g33500v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560487.1">
        <property type="nucleotide sequence ID" value="XM_003560439.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWG2"/>
    <dbReference type="EnsemblPlants" id="KQK17289">
        <property type="protein sequence ID" value="KQK17289"/>
        <property type="gene ID" value="BRADI_1g33500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838538"/>
    <dbReference type="Gramene" id="KQK17289">
        <property type="protein sequence ID" value="KQK17289"/>
        <property type="gene ID" value="BRADI_1g33500v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838538"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001724_2_3_1"/>
    <dbReference type="OMA" id="IKTCAEM"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000087">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000257">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48049:SF84">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 79A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK17289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="49750" checksum="BB2284AC3E22D024" modified="2012-06-13" version="1">MSSAVASAGGDGDGDIHVLMFPFLAFGHISPFVQLARKLVAGNNRVRVTLLSAAANVPRVEAMLGASAAAAVAVAPLQLPRVAGLPEGAESTAEVSADGAELLKIAVDGTRPQVAALLAELRPDAVLFDFATPWVTDIAAPLGIKALQFSVFAAVAGAYLMVQARRANNGSLPSTADELVSAPAGFPPSSALATVPAYQAADFKYVYTAFHGMPCVYDRVVAGIEACDALVVKTCAEMEGAYIDYLAAQFGKPVLVTGLVVPDPPQGELEEPWATWLSTFPDNAAVFASFGSETFLPAAAATELLLGLEASKRPFLAVLNFPRGADVEAELRKCVPPGFEERVKGRGAVHTGWVQQQHILRHASVGCFVNHAGLSSVVEGLVAGCRLVLLPMKGDQYLNAALAARELRVGVEVARRAEDGWFGRDDVAAAVAEAEADGGCREGKKWVEFLMDGAVQRRFVDDFIVGLRNLVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXJ3</accession>
    <name>I1GXJ3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">heme oxygenase (biliverdin-producing)</fullName>
            <ecNumber evidence="3">1.14.14.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100841507</name>
        <name evidence="12" type="ORF">BRADI_1g36640v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heme oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.18" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563666.1">
        <property type="nucleotide sequence ID" value="XM_003563618.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXJ3"/>
    <dbReference type="STRING" id="15368.I1GXJ3"/>
    <dbReference type="EnsemblPlants" id="KQK17770">
        <property type="protein sequence ID" value="KQK17770"/>
        <property type="gene ID" value="BRADI_1g36640v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841507"/>
    <dbReference type="Gramene" id="KQK17770">
        <property type="protein sequence ID" value="KQK17770"/>
        <property type="gene ID" value="BRADI_1g36640v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841507"/>
    <dbReference type="eggNOG" id="KOG4480">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063325_1_0_1"/>
    <dbReference type="OMA" id="PPEFICH"/>
    <dbReference type="OrthoDB" id="652091at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004392">
        <property type="term" value="F:heme oxygenase (decyclizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006788">
        <property type="term" value="P:heme oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010229">
        <property type="term" value="P:inflorescence development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048573">
        <property type="term" value="P:photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19165">
        <property type="entry name" value="HemeO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.910.10:FF:000005">
        <property type="entry name" value="Heme oxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002051">
        <property type="entry name" value="Haem_Oase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016053">
        <property type="entry name" value="Haem_Oase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016951">
        <property type="entry name" value="Haem_Oase_decyc_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35703">
        <property type="entry name" value="HEME OXYGENASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35703:SF2">
        <property type="entry name" value="HEME OXYGENASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01126">
        <property type="entry name" value="Heme_oxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK17770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="279" mass="31084" checksum="34F9310E5A635120" modified="2012-06-13" version="1">MAPAAAASLATPHTLAAPRVSVAVAGRNSGLSVSVGVRQVPGVSAVLSRRRMVATAAATEMAPAAKGEEGGKAFVEEMRAVAMRLHTKDQAREGEKEPQAPPVAKWEPTIEGYLRFLVDSKLVFQTLEDIVDRAAVPWYAEFRNTGLERSEQLKKDLEWFRQQGHTIPEPSAPGTTYASYLEELSEKDPQAFICHFYNVYFAHTAGGRMIGKKVAEKILNKKELEFYKWEGTLSQLLQNVRNKLNQVALSWSREEKDHCLEETEKSFAYSGDLLRQIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYQ6</accession>
    <name>I1GYQ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial glycoprotein family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100836936</name>
        <name evidence="1" type="ORF">BRADI_1g42640v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563895.1">
        <property type="nucleotide sequence ID" value="XM_003563847.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYQ6"/>
    <dbReference type="FunCoup" id="I1GYQ6">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GYQ6"/>
    <dbReference type="EnsemblPlants" id="KQK18465">
        <property type="protein sequence ID" value="KQK18465"/>
        <property type="gene ID" value="BRADI_1g42640v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836936"/>
    <dbReference type="Gramene" id="KQK18465">
        <property type="protein sequence ID" value="KQK18465"/>
        <property type="gene ID" value="BRADI_1g42640v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836936"/>
    <dbReference type="eggNOG" id="KOG2536">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072692_1_2_1"/>
    <dbReference type="OMA" id="CEYMMSK"/>
    <dbReference type="OrthoDB" id="278212at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.280.10:FF:000009">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF27">
        <property type="entry name" value="OS06G0326500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK18465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="30107" checksum="AE824BFED6E3D6FB" modified="2012-06-13" version="1">MSSGSLAVRLLRRATSSSQLSTTLTRRCAHSYAASRPPSPLPRFHSAAAAASSHSGITIRRFLSSQSQVSSSKVSADENLRRVIDSEIECVVESEEASAQQIDPPEDFPFEIIDNPGDQSVILKREFAGETIKATVYTNFDAEEDLNNDDSDAEKDEESFKPAIQMVVTVEKPEGPILEFDCNFNDDELAIESMRMLNRDNNDAENVYEGPPFSILDDSLQKSLHRYLEVRGIKHSLHDWLHEFMMSKDEKEYVVWLKNMRDFIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZ44</accession>
    <name>I1GZ44_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100835286</name>
        <name evidence="10" type="ORF">BRADI_1g43800v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560823.2">
        <property type="nucleotide sequence ID" value="XM_003560775.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZ44"/>
    <dbReference type="EnsemblPlants" id="KQK18639">
        <property type="protein sequence ID" value="KQK18639"/>
        <property type="gene ID" value="BRADI_1g43800v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835286"/>
    <dbReference type="Gramene" id="KQK18639">
        <property type="protein sequence ID" value="KQK18639"/>
        <property type="gene ID" value="BRADI_1g43800v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835286"/>
    <dbReference type="eggNOG" id="ENOG502S0YE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_21_4_1"/>
    <dbReference type="OMA" id="LMFHENE"/>
    <dbReference type="OrthoDB" id="2017960at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051258">
        <property type="entry name" value="Diverse_Substrate_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920:SF7">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920">
        <property type="entry name" value="OS03G0707200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="9">
        <location>
            <begin position="146"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="9">
        <location>
            <begin position="281"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="27"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK18639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="451" mass="49630" checksum="77E746473F8007FC" modified="2012-06-13" version="1">MCYHSKQHVLLKIVSLCPSSGEMLLSKLPNPPRPWRPRSLETHPRRHRQLPLPNVPVMPVPRRRQLLSPSAASSDYLSEDPELPPAKRFSSKKALGESGRWKVVPPKTPKPSTPDPQEPPSWFTVRRQAHAAWRKVTLLVPSRARSVILLNLVVLIFASNISVVKEAQSMLDPDLFNVLRFSIAAIPFVPFLLKSLRDMQVFIRGVELGIWVTLAFLAQSIGLVTADAGRASFISALTVIIVPLLDGILGAEIPIYTWLGALLSMVGVGILELSGSPPCVGDLLTLLSAFCFAIHMLRTEHISRNMKKDNFLALVGCQVLVLALVSAASFIVKRLLQSMVHWNLKSQTPTQLFSMMAAFPWLAILYTGILSTTFCLWAEVVAMRDVSATETAIIYGLEPVWGAAFAWAIHGERWGVTGLIGAIFIIAGSLMVQILGSFLDVSGEDSYQMNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZW7</accession>
    <name>I1GZW7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100828066</name>
        <name evidence="8" type="ORF">BRADI_1g46180v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564114.2">
        <property type="nucleotide sequence ID" value="XM_003564066.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZW7"/>
    <dbReference type="STRING" id="15368.I1GZW7"/>
    <dbReference type="EnsemblPlants" id="PNT76231">
        <property type="protein sequence ID" value="PNT76231"/>
        <property type="gene ID" value="BRADI_1g46180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828066"/>
    <dbReference type="Gramene" id="PNT76231">
        <property type="protein sequence ID" value="PNT76231"/>
        <property type="gene ID" value="BRADI_1g46180v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828066"/>
    <dbReference type="eggNOG" id="KOG0954">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004226_1_0_1"/>
    <dbReference type="OMA" id="TTCDVCC"/>
    <dbReference type="OrthoDB" id="20839at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GZW7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15571">
        <property type="entry name" value="ePHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15495">
        <property type="entry name" value="PHD_ATX3_4_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042011">
        <property type="entry name" value="ATX3/4/5_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF131">
        <property type="entry name" value="PHD-FINGER DOMAIN CONTAINING PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="193"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="260"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="868"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="937"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1118"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1207"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1124"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1215"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1249"/>
            <end position="1266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT76231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1277" mass="141502" checksum="9E63EB182E8797F7" modified="2012-06-13" version="1">MEAYFREFTLDDFQALEPKPLFGPNWLDPCLLLPFVVGGKKLGEYSDPSDVAVIAENSDSNLILGKENEEMQNKNDMVHSNLDTQEGSCSVGCNVEVVNNNGINEGHCEQDMQEVILQQEEQPGEIELDQGKGHAVVLPGCKPNLDGSLNWLLGAKDRFVLTSERPNKKRKLLGADAGLDRLVLLPSLEGEAGSRCDVCSLGESDTASNRLLKCNSCEVSVHQKCYGVQVVPDGYWMCAWCNSSWLARRLTRSDAGTTVFMPCVLCPKDKGALKPVKWEPGRTADGGNINFAHLFCSLWAPEVLVEDMDSMEPITNVGDIQENRTKMVCGVCKIMHGACLRCSHGTCRACFHPICAREAKHHMEIWGKSGHTNVEMRAFCAKHSAARSINSLHNVNGVAEHDTPQVGLADENLTSDEKQQMRFTRKSKEKLLNDTFVSSSLSSLNKVQTTEVVSSQVRSVESQQIQHSDMVVDRTTRDEKLVSNSGDVSTVLRKLIDQGKVSVGDIESELGLSSESLEAALVPETTTTSPGLKLKIIKLLQNSVHVPSVQVKSLKEGSMTPQGTLPRIESKNLTDSQLGSELEEGISSFDYCFPEGYKAKDSADSVENCSQNCPDFDDDHISGQCIFNIDGYRCYIHPSVEKKLRSLRDQIKNKKDQAVYYDVEGLLCPRRGADLGGSSMKLEQLTDIAAADQASKAESSGILEYSPHDEIEGEIVYLQSRLLDDVVAMNRRYDDIRFKVVQNLSHELDSFNKRKWDHIIVNQFLRDIREAKKRGNTERRHKEAQAILAAAAPYVAHNPRNVTVIKEAENDVAPAKQEIIPKVNAGSLRVSQLASLPQTKDPSFSNSKVSADTNFGFFDLAKFSKKNGLPCDVCMRSETVLNRIFLCSSCKAAVHLDCYRSRTNPTGPWKCELCEETLSDAVISGMQSDCSGAKSFLVQCCLCDGTSGAFRKTTKGKWVHAFCAEWLLENTFKRGQYNAVGGTESLLKGKDTCSICHHSVGTCLKCGTVGCQVAFHPACARDAGLYMNTKKVGSLWRHKAYCGNHSIEQRKVDSQQYGPAEVKIMKQMRVELERLRLICERIVKREKEKKEVVVCEHDILAARRDHIALSTRSLYYTSGPGGASSESATTSVNNNSYSGKRQRSDEENVTVRSDDVTVDSTITRKHTVRFSVHSRETDRNTADSSTSTISYKRKLDDGESLADKDLQEGGAIASEKSEGETKSVDKKHEETFQKELVKTSDQDMTQKQHPPKRLVYTRRSSSKKKERIQDVQVPGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2J1</accession>
    <name>I1H2J1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein LURP-one-related 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100823295</name>
        <name evidence="2" type="ORF">BRADI_1g53710v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557363.1">
        <property type="nucleotide sequence ID" value="XM_003557315.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2J1"/>
    <dbReference type="STRING" id="15368.I1H2J1"/>
    <dbReference type="EnsemblPlants" id="KQK20307">
        <property type="protein sequence ID" value="KQK20307"/>
        <property type="gene ID" value="BRADI_1g53710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823295"/>
    <dbReference type="Gramene" id="KQK20307">
        <property type="protein sequence ID" value="KQK20307"/>
        <property type="gene ID" value="BRADI_1g53710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823295"/>
    <dbReference type="eggNOG" id="ENOG502QUU9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063146_5_1_1"/>
    <dbReference type="OMA" id="VIGPEYC"/>
    <dbReference type="OrthoDB" id="97518at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF58">
        <property type="entry name" value="OS07G0230700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK20307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="22405" checksum="43E936161F106EE8" modified="2012-06-13" version="1">MAAAAAASGAPPAPLPVVGQQFCAPYVVPLTVTKKAISLSDGDFAITDANGAVVLKVKGAIFSIRNRRVLLDAAGQPLLSMQEKVFSMHNRWEVFRGDSTNASDLLFTVKKSSMIQLKTEMDVFLAGNTAQQVCDFKIKGSYFDRSCAFYLGNSSTMVAQMNRKFTVSNVLLGKDTFGVTVFPHVDYVFIATLVVILDEVHRDKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2Q4</accession>
    <name>I1H2Q4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100842709</name>
        <name evidence="7" type="ORF">BRADI_1g54290v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228236.1">
        <property type="nucleotide sequence ID" value="XM_010229934.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228237.1">
        <property type="nucleotide sequence ID" value="XM_010229935.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2Q4"/>
    <dbReference type="STRING" id="15368.I1H2Q4"/>
    <dbReference type="EnsemblPlants" id="KQK20400">
        <property type="protein sequence ID" value="KQK20400"/>
        <property type="gene ID" value="BRADI_1g54290v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK20401">
        <property type="protein sequence ID" value="KQK20401"/>
        <property type="gene ID" value="BRADI_1g54290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842709"/>
    <dbReference type="Gramene" id="KQK20400">
        <property type="protein sequence ID" value="KQK20400"/>
        <property type="gene ID" value="BRADI_1g54290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20401">
        <property type="protein sequence ID" value="KQK20401"/>
        <property type="gene ID" value="BRADI_1g54290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842709"/>
    <dbReference type="eggNOG" id="ENOG502S0SZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076461_0_0_1"/>
    <dbReference type="OMA" id="PCLRAFS"/>
    <dbReference type="OrthoDB" id="1414122at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF5">
        <property type="entry name" value="OS07G0206800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="6">
        <location>
            <begin position="134"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK20400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="252" mass="27239" checksum="5356ADB5626D5573" modified="2012-06-13" version="1">MASAAAADEKPSAHLAHPLLGQPQHPHPCYAYPAATYAYAPAPPPPPPPPTLFVLPTSPVFVRLRRLRPRRIPCIRRFCTRTLPLLLALALLAGLAFLLYPSAPAARVADIRLDRFRVNPPPLPALDFNLALRLRVHNPGFLLPLRYRAVSSAVSYRGRLLGSATARPGSGELAARGDTYAYSEVWVDAGKVLDDVIELIGDIAAGSVPLEIVTEVIGAVRVFRFDIPVKGLISCSVNVSPDTQSIISQDCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>I1H646</accession>
    <name>I1H646_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100824836</name>
        <name evidence="11" type="ORF">BRADI_1g64190v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228700.1">
        <property type="nucleotide sequence ID" value="XM_010230398.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H646"/>
    <dbReference type="FunCoup" id="I1H646">
        <property type="interactions" value="2822"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H646"/>
    <dbReference type="EnsemblPlants" id="KQK21953">
        <property type="protein sequence ID" value="KQK21953"/>
        <property type="gene ID" value="BRADI_1g64190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824836"/>
    <dbReference type="Gramene" id="KQK21953">
        <property type="protein sequence ID" value="KQK21953"/>
        <property type="gene ID" value="BRADI_1g64190v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824836"/>
    <dbReference type="eggNOG" id="KOG0925">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_5_11_1"/>
    <dbReference type="OMA" id="MKVYPLY"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H646">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17973">
        <property type="entry name" value="DEXHc_DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000003">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:006066">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000951">
        <property type="entry name" value="Putative pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044756">
        <property type="entry name" value="DHX15_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF109">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="72"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="261"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK21953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="719" mass="81600" checksum="ED79E8A2F7034B93" modified="2012-06-13" version="1">MGTERKRKVSLFDVVDETSVSAKLGRAGVNGAAAAANPSINKWNGRPYSARYLEILEKRRTLPVWQQKEEFLRALRDNQTLILVGETGSGKTTQIPQFVLEAEGLSNRSMVACTQPRRVAAMSVSRRVAEEMDVTIGEEVGYSIRFEDCSSHKTVLKYLTDGMLLREAMADPLLERYKVIVLDEAHERTLATDVLFGLLKEVLKNRPDLKLVVMSATLEAEKFQGYFSSAPLMKVPGRLHPVEIFYTQEPERDYLEAAIRTVVQIHMCEPAGDILVFLTGEEEIEDACRKINKEVNNMGDQVGPVKVVPLYSTLPPAMQQKIFEPAPAPLKEGGPPGRKIVVSTNIAETSLTIDGIVYVIDPGFSKQKVYNPRIRVESLLVSPISKASAHQRAGRAGRTQPGKCFRLYTEKSFNDDLHPQTYPEILRSNLANTVLTLKKLGIDDLVHFDFMDPPAPETLMRALEVLNYLGALDDEGNLTSLGEMMSEFPLDPQMSKMLVISPRYNCSNEILSISAMLSVPNCFLRPREAQKAADEAKARFGHIDGDHLTLLNVYHAYKQNNEDPTWCYENFVNARAMKNADNVRQQLVRIMTRFNLRMCSTDFNSREYYVNIRKAMLSGYFMQVAHLERTGHYLTVKDNQVVHLHPSNCMDHKPEWVIYNEYVLTTRNFIRTVTDIRGEWLIDIAPQYYDLTNFPTCEAKRVLERLHNKRERERAASRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HBG7</accession>
    <name>I1HBG7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Association with the SNF1 complex (ASC) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_2g01490v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HBG7"/>
    <dbReference type="FunCoup" id="I1HBG7">
        <property type="interactions" value="633"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HBG7"/>
    <dbReference type="EnsemblPlants" id="KQK02446">
        <property type="protein sequence ID" value="KQK02446"/>
        <property type="gene ID" value="BRADI_2g01490v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02446">
        <property type="protein sequence ID" value="KQK02446"/>
        <property type="gene ID" value="BRADI_2g01490v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070949_3_1_1"/>
    <dbReference type="InParanoid" id="I1HBG7"/>
    <dbReference type="OMA" id="NNVYPGH"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043554">
        <property type="entry name" value="KINB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316">
        <property type="entry name" value="SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316:SF5">
        <property type="entry name" value="SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="3">
        <location>
            <begin position="20"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK02446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="111" mass="12410" checksum="256EC14F99AAAD6D" modified="2012-06-13" version="1">MDPQGREDHEGVRVVGFEVPTSPDSSYSNPIPGNEDEAREPPLVPPHLQHTLLSFPPSHDDSSSLPPPQPVVLNHLYIEKENSRSVVALGITHRFKAKFVTVVLYKPVQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HC22</accession>
    <name>I1HC22_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 13">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100821089</name>
        <name evidence="14" type="ORF">BRADI_2g03330v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Endoplasmic reticulum membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 13"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HC22"/>
    <dbReference type="STRING" id="15368.I1HC22"/>
    <dbReference type="EnsemblPlants" id="KQK02730">
        <property type="protein sequence ID" value="KQK02730"/>
        <property type="gene ID" value="BRADI_2g03330v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02730">
        <property type="protein sequence ID" value="KQK02730"/>
        <property type="gene ID" value="BRADI_2g03330v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="FHYLIVQ"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018812">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF38">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK02730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="248" mass="28440" checksum="BA6DF7EDC52FD9A8" modified="2012-06-13" version="1">MLKIKNINCTIAASSSSLVDLYLQTAIYSSAMAALRRLYLSVYNWAVCFGWVQVLYNAASALLESRHDAVYPAVERPLLFAQTTAFLEILHSILGFVRSPISVTLPQISGRLYVTWGILWSFPETHSHILVTSLIISWSITEIIRYSFFGMREAFGFAPSWLLWLRYSTFLVFYPIGLISEVGLIYVAMPSMKASEKYCFRMPNKWNLSFDYRYMSVLLTALYVPGFPYLFRYMVAKREKALSKTKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCM3</accession>
    <name>I1HCM3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100843464</name>
        <name evidence="2" type="ORF">BRADI_2g04980v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569749.1">
        <property type="nucleotide sequence ID" value="XM_003569701.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCM3"/>
    <dbReference type="STRING" id="15368.I1HCM3"/>
    <dbReference type="EnsemblPlants" id="KQK03007">
        <property type="protein sequence ID" value="KQK03007"/>
        <property type="gene ID" value="BRADI_2g04980v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843464"/>
    <dbReference type="Gramene" id="KQK03007">
        <property type="protein sequence ID" value="KQK03007"/>
        <property type="gene ID" value="BRADI_2g04980v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843464"/>
    <dbReference type="eggNOG" id="ENOG502SK9M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014546_2_0_1"/>
    <dbReference type="OMA" id="AWFIDAT"/>
    <dbReference type="OrthoDB" id="444127at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF41">
        <property type="entry name" value="OS01G0179000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="437" mass="46658" checksum="F10B73CAA6B60E05" modified="2012-06-13" version="1">MAAANKSVSRLPQRLVLPAEPTPAGPLRLSWLDRYPTQMALIESLHVFKPAPAHDGADPARTIERALAQALVRYYPLAGRLAFTDDGGQSHVDCGGPGSGVWFTEAAAACGLEDVDYLEHPMMIPKDALLPPTPHAAEEGDERRLVLLVQVTSFACGGFVVGFRFSHAVADGPGAAQFMAAVGDLARGAESLSMEPQWGRDAVPDPAGAVVGALPDPAGAKRLEYLAMDISADYIDHFKSQYNSSNNGGGARCSAFEVLVAKAWQSRTRAAGFDPSATVHLCFAMNARPLLHASLPSAGAGFYGNCYYIMRVSAPAGKVSGSSVPEVVKIIKDGKRRMPAEFARWASGEAGAGGEDPYRITSDYRTLLVSDWTRLGFAEVDYGWGPPAHVVPLTNLDYIATCILVKPWAHKPGARLITQCVTPDRVAAFHQGMLDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HD41</accession>
    <name>I1HD41_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100834783</name>
        <name evidence="3" type="ORF">BRADI_2g06440v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565238.1">
        <property type="nucleotide sequence ID" value="XM_003565190.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HD41"/>
    <dbReference type="FunCoup" id="I1HD41">
        <property type="interactions" value="936"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HD41"/>
    <dbReference type="EnsemblPlants" id="KQK03231">
        <property type="protein sequence ID" value="KQK03231"/>
        <property type="gene ID" value="BRADI_2g06440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834783"/>
    <dbReference type="Gramene" id="KQK03231">
        <property type="protein sequence ID" value="KQK03231"/>
        <property type="gene ID" value="BRADI_2g06440v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834783"/>
    <dbReference type="eggNOG" id="ENOG502QQ86">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057939_1_0_1"/>
    <dbReference type="OMA" id="GDLMVWG"/>
    <dbReference type="OrthoDB" id="2161379at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HD41">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45296">
        <property type="entry name" value="TRANSDUCIN/WD40 REPEAT-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45296:SF1">
        <property type="entry name" value="TRANSDUCIN_WD40 REPEAT-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="151"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="294"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK03231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="40842" checksum="A65F6879CEBDEE79" modified="2012-06-13" version="1">MSETGAAAGASSTERKPRRLRGHKKGAVTCCVASSARPGVVVSSGEDGCLCWFDLRTKDVLLTIEATNKAISSVCFKAGNEDFVYVSAGNEILSFDVRMGSQSKPLETYNYNREEINQIAVSSKGFLAAADDSGDVKIVNTMQKCLYKRLREAHTSICSSVQFIPWRPWTAITGGLDSKLAVWDFSKGRTLFSIDYGSPEMQNRSSSASAGQCLNPAFIHSVAVSEEGGLYKVCAVARGDGAVDVVDLEYELAPAKSKGPSRAGGSAMSSKGTDLGNGSSNQSQQKRILLDYTMGGHTAAVSCVAFSAFGEKGKFLVSGGNDASVKLWDWSKGFSSETNSNSELVLDIDVKKKVNWLCTAPTDSDNLIVCDTSKVVKVYNFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HFV1</accession>
    <name>I1HFV1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100824063</name>
        <name evidence="7" type="ORF">BRADI_2g14580v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PER33/POM33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230961.1">
        <property type="nucleotide sequence ID" value="XM_010232659.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HFV1"/>
    <dbReference type="FunCoup" id="I1HFV1">
        <property type="interactions" value="1074"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HFV1"/>
    <dbReference type="EnsemblPlants" id="KQK04605">
        <property type="protein sequence ID" value="KQK04605"/>
        <property type="gene ID" value="BRADI_2g14580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824063"/>
    <dbReference type="Gramene" id="KQK04605">
        <property type="protein sequence ID" value="KQK04605"/>
        <property type="gene ID" value="BRADI_2g14580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824063"/>
    <dbReference type="OrthoDB" id="2017147at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HFV1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005344">
        <property type="entry name" value="TMEM33/Pom33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF7">
        <property type="entry name" value="OS05G0589000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03661">
        <property type="entry name" value="TMEM33_Pom33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="82"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK04605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="358" mass="40389" checksum="BAB538EA7AF68577" modified="2012-06-13" version="1">MGGGDASGLGAGPEREQEEEQARKRAAAAGYDYEGDARWAEYWSNVLVPPHLASRPDVVGHLQRKFYQRYIDRDLVVEPMSSIGSTQASRPEVRSSSSASSESVRPRNSGSSSRSAAPPPPPPQTDRAANPLRFDARTIHFSINAWVLVVAGLGMLPILPKHLADRACKLSLLGTILSSGYSLYSTYGKPRALNMQAIQGWLNSVLGTKDFIHLMFSLLLFTSQLHLKIAALPVFCWALDHVARFLRRNFTHSSFYRAYLEEPCLWVETNNTTLSLLSSNAELALGFLLIISLFSWRRNIIQTFMYWNLLKLMYRAPVTSSYHQSTWAKIGRTVNPYIHRYAPFLQTPISTIQRWWLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I1HHW9</accession>
    <name>I1HHW9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_2g20620v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HHW9"/>
    <dbReference type="EnsemblPlants" id="KQK05532">
        <property type="protein sequence ID" value="KQK05532"/>
        <property type="gene ID" value="BRADI_2g20620v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05532">
        <property type="protein sequence ID" value="KQK05532"/>
        <property type="gene ID" value="BRADI_2g20620v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2295569_0_0_1"/>
    <dbReference type="InParanoid" id="I1HHW9"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48052:SF69">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FLS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48052">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK05532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="10916" checksum="BE81FD1AF89EE232" modified="2012-06-13" version="1">MGGRLPSRFGSMLPRNLSHLFLADNYQIKGSIPADMGDLKNLMLVEMNLSSNQLNGTTIPASICAFQNLRLLNLSNNALTGEIPPCIYRPSQDTQLAGPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJ35</accession>
    <name>I1HJ35_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF1682 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100839913</name>
        <name evidence="8" type="ORF">BRADI_2g24290v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568385.1">
        <property type="nucleotide sequence ID" value="XM_003568337.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJ35"/>
    <dbReference type="FunCoup" id="I1HJ35">
        <property type="interactions" value="2124"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HJ35"/>
    <dbReference type="EnsemblPlants" id="KQK06074">
        <property type="protein sequence ID" value="KQK06074"/>
        <property type="gene ID" value="BRADI_2g24290v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK06075">
        <property type="protein sequence ID" value="KQK06075"/>
        <property type="gene ID" value="BRADI_2g24290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839913"/>
    <dbReference type="Gramene" id="KQK06074">
        <property type="protein sequence ID" value="KQK06074"/>
        <property type="gene ID" value="BRADI_2g24290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06075">
        <property type="protein sequence ID" value="KQK06075"/>
        <property type="gene ID" value="BRADI_2g24290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839913"/>
    <dbReference type="eggNOG" id="KOG2357">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024226_0_0_1"/>
    <dbReference type="OMA" id="MHLVRDM"/>
    <dbReference type="OrthoDB" id="10039147at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012879">
        <property type="entry name" value="CCDC47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883">
        <property type="entry name" value="ADIPOCYTE-SPECIFIC PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07946">
        <property type="entry name" value="CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094663" description="DUF1682 family protein" evidence="7">
        <location>
            <begin position="29"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="45"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="464"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
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        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="475"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK06074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="53848" checksum="93687D82E0C26D60" modified="2012-06-13" version="1">MAAASWIRLPRATLILLLLALHLSLSLAAQFEGFDSDELPSASAADLASSADDDEELDLEVDLPPPPRISLSTAAPSPPVTTTTAANPNLNPSPTPTPPNPTPSLDFWDEDEFEGIPVPEGAISDESSTPADASPSDPSSEDAAEALPAPPRRPAELIRAFSIEIACVSFLICFLLNYFTGKRQNEAIALAWATKFATRDSIFDKNFSLLGTGDGKDTPLLLKEGQDVFKFYASGRRFCQGMLATMEMRARHDLLSKLVELVFPRKDTITFEVVMNEEAMDHVMVAVARKRVAKAMQKEERDLQKFASVITSAPAGRKWVADELAVVAESKEVAGDMITEAVLDQVLGEKAFEKFGKWFISLHFSDQLAGSYKKVLAFKFVLPDANNMAEMTRLVALVPFYIDLIGRYKLSSHARSKTDAARTKAAQEAFRELQGVRQEALQRKKAEKKKLLEEAEAKLSAEVLRKKEEKERSRQMKKGMPKVKMLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1HLW4</accession>
    <name>I1HLW4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g36090v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="I1HLW4"/>
    <dbReference type="EnsemblPlants" id="KQK07535">
        <property type="protein sequence ID" value="KQK07535"/>
        <property type="gene ID" value="BRADI_2g36090v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07535">
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    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R42N">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1391950_0_0_1"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
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        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK07535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="21666" checksum="5D79AC6B15EE5D68" modified="2012-06-13" version="1">MSRSMAPAAAGTTMSVLGVDLLQEIFIHLPDPGDLLRSAAACKPFLRAVRSARFLRRFRRRHSSTFPPLLGCILLRRSHNRGSLENHPVYLLTLSAAARRVVEGGDFALSFLPHRKLRCGAPWNVLDCRNGRLMLCSPVSRELVVADPLTRHCVSLPVLPAMLLIGNGLVTDYDEYSCSRRSAFREAVPPPGFAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPA7</accession>
    <name>I1HPA7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100846813</name>
        <name evidence="5" type="ORF">BRADI_2g43190v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569336.1">
        <property type="nucleotide sequence ID" value="XM_003569288.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPA7"/>
    <dbReference type="STRING" id="15368.I1HPA7"/>
    <dbReference type="EnsemblPlants" id="KQK08675">
        <property type="protein sequence ID" value="KQK08675"/>
        <property type="gene ID" value="BRADI_2g43190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846813"/>
    <dbReference type="Gramene" id="KQK08675">
        <property type="protein sequence ID" value="KQK08675"/>
        <property type="gene ID" value="BRADI_2g43190v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846813"/>
    <dbReference type="eggNOG" id="KOG3138">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_5_3_1"/>
    <dbReference type="OMA" id="ICCRLET"/>
    <dbReference type="OrthoDB" id="47374at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HPA7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000006">
        <property type="entry name" value="Putative n-alpha-acetyltransferase 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF8">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="26"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK08675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="181" mass="19819" checksum="27466578A881A693" modified="2012-06-13" version="1">MGAGEGEAAGSKDKSGGGGAGGVARTSLDGLRDKNVMQLRKLNLALFPVRYNDKYYQDAIASKDFSKLAYYSDICVGAIACRLEKKEGGAVRVYIMTLGVLAPYRGLGLGTKLLNHVFDLCAKQNIPEIYLHVQTNNDDAIAFYKKFGFEITETIHKYYTNITPPDCYVLTKFIGQAATKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HWV9</accession>
    <name>I1HWV9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100833698</name>
        <name evidence="4" type="ORF">BRADI_3g02920v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570881.1">
        <property type="nucleotide sequence ID" value="XM_003570833.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HWV9"/>
    <dbReference type="STRING" id="15368.I1HWV9"/>
    <dbReference type="EnsemblPlants" id="KQJ93133">
        <property type="protein sequence ID" value="KQJ93133"/>
        <property type="gene ID" value="BRADI_3g02920v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833698"/>
    <dbReference type="Gramene" id="KQJ93133">
        <property type="protein sequence ID" value="KQJ93133"/>
        <property type="gene ID" value="BRADI_3g02920v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833698"/>
    <dbReference type="eggNOG" id="ENOG502S1MD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126789_0_0_1"/>
    <dbReference type="OMA" id="CTIPWEA"/>
    <dbReference type="OrthoDB" id="674290at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
        <property type="term" value="P:regulation of DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040389">
        <property type="entry name" value="SMR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33142">
        <property type="entry name" value="CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33142:SF40">
        <property type="entry name" value="CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ93133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="151" mass="16139" checksum="8CFB96E64EA43514" modified="2012-06-13" version="1">MGFQDHLEKKPQPLRHHGAALAEDKTAAGSGRKKKKKAAAVWIALPPLRPIKVGRRRRGNGGAGAREEEEEDEEVTTPTGECCRIPAEAAACPPAPRKKQKTAVAIVGGGDRRCSSSSRDVYGEPATEEYFRVPADLEAVFAAVGRVAQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZI3</accession>
    <name>I1HZI3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tocopherol cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100846924</name>
        <name evidence="2" type="ORF">BRADI_3g10250v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234137.1">
        <property type="nucleotide sequence ID" value="XM_010235835.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZI3"/>
    <dbReference type="STRING" id="15368.I1HZI3"/>
    <dbReference type="EnsemblPlants" id="PNT66331">
        <property type="protein sequence ID" value="PNT66331"/>
        <property type="gene ID" value="BRADI_3g10250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846924"/>
    <dbReference type="Gramene" id="PNT66331">
        <property type="protein sequence ID" value="PNT66331"/>
        <property type="gene ID" value="BRADI_3g10250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846924"/>
    <dbReference type="eggNOG" id="ENOG502QQ9P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048962_0_0_1"/>
    <dbReference type="OMA" id="PHSGYHW"/>
    <dbReference type="OrthoDB" id="38968at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010287">
        <property type="term" value="C:plastoglobule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009976">
        <property type="term" value="F:tocopherol cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015994">
        <property type="term" value="P:chlorophyll metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009915">
        <property type="term" value="P:phloem sucrose loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009644">
        <property type="term" value="P:response to high light intensity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009266">
        <property type="term" value="P:response to temperature stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010189">
        <property type="term" value="P:vitamin E biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016122">
        <property type="term" value="P:xanthophyll metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025893">
        <property type="entry name" value="Tocopherol_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35309">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35309:SF2">
        <property type="entry name" value="TOCOPHEROL CYCLASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14249">
        <property type="entry name" value="Tocopherol_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="52169" checksum="3ADF051A5D76B8E9" modified="2012-06-13" version="1">MGLVTVAAAVVPGATPVPRAPRPRPRWCPRPSRSFTTRAAAEPAAAAPAPVYAPTPRDRPLRTPHSGYHYDGTARPFFEGWYFKVSIPEVRQSFCFMYSVENPFFRDGMSALDRILYGPRFTGVGAQILGADDKYICQFSEKSNNFWGSRHELMLGNTFIPSKGSTSPDGEVPPQEFSNRVLEGYQVTPIWHQGFIRDDGRSKYVPNVQTARWEYSTRPVCGWGDVTSKQKSTAGWLAAFPLFEPHWQICMAGGVSTGWIEWDGERFEFENAPSYSEKNWGGGFPRKWYWIQCNVFSGASGEVALTAAGGLRKIGLGDTYESPSLIGVHYDGVFYEFVPWTGTVSWDIAPWGQWKMSGENKSHLVEIEATTKEPGTTLRAPTMEAGLVPACKDTCYGDLKLQLWEKRHDGGKGKMILDTTSNMAALEVGGGPWFNEWKGTTVSNELVNSIIGTQVDVDSLLLIPVLKPPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1Y3</accession>
    <name>I1I1Y3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100836881</name>
        <name evidence="4" type="ORF">BRADI_3g17960v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573552.1">
        <property type="nucleotide sequence ID" value="XM_003573504.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1Y3"/>
    <dbReference type="FunCoup" id="I1I1Y3">
        <property type="interactions" value="3173"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I1Y3"/>
    <dbReference type="EnsemblPlants" id="KQJ95590">
        <property type="protein sequence ID" value="KQJ95590"/>
        <property type="gene ID" value="BRADI_3g17960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836881"/>
    <dbReference type="Gramene" id="KQJ95590">
        <property type="protein sequence ID" value="KQJ95590"/>
        <property type="gene ID" value="BRADI_3g17960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836881"/>
    <dbReference type="eggNOG" id="KOG3151">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046003_0_0_1"/>
    <dbReference type="OMA" id="HIMDGYF"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I1Y3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000001">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006746">
        <property type="entry name" value="26S_Psome_Rpn12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="79"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ95590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="267" mass="30598" checksum="79861A87954DB70B" modified="2012-06-13" version="1">MDPKLTEVTQLFARFKAAYSRNDLDVCANLLSQLKVLLTKFPSLPPSFQQTPNAVEELKLARDIYEHAVVLSVKMEDQDAFERDFCQLKPYYMDTCGIIPPSPEEYPILGLNLLRLLVQNRIAEFHTELELLPAKALDNLCIKHAVELEQSFMEGAYNRVLSARQAVPHETYVYFMDLLAKTVRDEIAGCSEKGYDYLSISDAKEMFMFSSDQELQQYITEEHPEWDVKNGSVFFQKAKESQPCKEIPAAPVINQTLGYARELERIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8W4</accession>
    <name>I1I8W4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g41180v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8W4"/>
    <dbReference type="STRING" id="15368.I1I8W4"/>
    <dbReference type="EnsemblPlants" id="KQJ99110">
        <property type="protein sequence ID" value="KQJ99110"/>
        <property type="gene ID" value="BRADI_3g41180v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99110">
        <property type="protein sequence ID" value="KQJ99110"/>
        <property type="gene ID" value="BRADI_3g41180v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RGJD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116935_0_0_1"/>
    <dbReference type="InParanoid" id="I1I8W4"/>
    <dbReference type="OMA" id="HMPIALN"/>
    <dbReference type="OrthoDB" id="64318at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I8W4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037360">
        <property type="entry name" value="COMMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15663">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15663:SF4">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ99110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="209" mass="23345" checksum="59E8344E89589876" modified="2012-06-13" version="1">MEERRPAAAAALWGHGHLPLLARARSKDSVEYVLQALWRTRRTGLDAADRAVVRDILQIPSDSELDPLLVCLRILIRRCVHDDVAKDDIPKLFPEEVLPELQRLLTLLLQKFQPEWRQDTAKDQAPAQNSERTKCHLSQNQAASEQPAITEPHCGTSSVEDSSKSGQKEVKLRLAKDSLDKMLKDMYPNKEQVSNAADVNGHEEAAGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1IAQ4</accession>
    <name>I1IAQ4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100835431</name>
        <name evidence="11" type="ORF">BRADI_3g46330v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572637.1">
        <property type="nucleotide sequence ID" value="XM_003572589.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IAQ4"/>
    <dbReference type="EnsemblPlants" id="KQJ99963">
        <property type="protein sequence ID" value="KQJ99963"/>
        <property type="gene ID" value="BRADI_3g46330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835431"/>
    <dbReference type="Gramene" id="KQJ99963">
        <property type="protein sequence ID" value="KQJ99963"/>
        <property type="gene ID" value="BRADI_3g46330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835431"/>
    <dbReference type="eggNOG" id="ENOG502S98H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1519918_0_0_1"/>
    <dbReference type="OMA" id="HTKRDYG"/>
    <dbReference type="OrthoDB" id="682142at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573:SF3">
        <property type="entry name" value="CASP-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573">
        <property type="entry name" value="CASP-LIKE PROTEIN 4A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095220" description="CASP-like protein" evidence="9">
        <location>
            <begin position="34"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="10">
        <location>
            <begin position="13"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ99963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="176" mass="18930" checksum="BFA40504265C5BD4" modified="2012-06-13" version="1">MAMSRRTAWIGGGLLARLLMVAVLLMSVRFVLANHTDLDYPGGYNKLQSYAYVVASAIVGVAGSVLQIPVALYLLCKSKRAMPSAMILDVSMHADIVISVVLASGVGAGFGATNDVLRYVRVIEWKGGSSERQDLTDYYNRAIVALIFLLVGMLLSFCATVVSARLRAKAINESEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1ID83</accession>
    <name>I1ID83_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100838295</name>
        <name evidence="5" type="ORF">BRADI_3g53410v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570245.1">
        <property type="nucleotide sequence ID" value="XM_003570197.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ID83"/>
    <dbReference type="STRING" id="15368.I1ID83"/>
    <dbReference type="EnsemblPlants" id="KQK01032">
        <property type="protein sequence ID" value="KQK01032"/>
        <property type="gene ID" value="BRADI_3g53410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838295"/>
    <dbReference type="Gramene" id="KQK01032">
        <property type="protein sequence ID" value="KQK01032"/>
        <property type="gene ID" value="BRADI_3g53410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838295"/>
    <dbReference type="eggNOG" id="KOG3225">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114728_0_0_1"/>
    <dbReference type="OMA" id="REKKDFW"/>
    <dbReference type="OrthoDB" id="1913277at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ID83">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF18">
        <property type="entry name" value="OUTER ENVELOPE PORE PROTEIN 16-3, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK01032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="148" mass="15527" checksum="BEB7B6C944BFBB65" modified="2012-06-13" version="1">MEDDSPVVKTAKGAMTGLAAGTIWGTIVATWHDVPRVERHVALPGLIRTVKMCGSYGVTFAAVGGLYIGVEQLVQAQRKKRDFVNGAIGAFVAGATVYGYRGRSIKSALIGGSSLAFTSAVLDVGGNTTRVDNGKAYHAYTTEKKPAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEV5</accession>
    <name>I1IEV5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100834410</name>
        <name evidence="2" type="ORF">BRADI_3g58000v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570569.1">
        <property type="nucleotide sequence ID" value="XM_003570521.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEV5"/>
    <dbReference type="FunCoup" id="I1IEV5">
        <property type="interactions" value="1505"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IEV5"/>
    <dbReference type="EnsemblPlants" id="KQK01754">
        <property type="protein sequence ID" value="KQK01754"/>
        <property type="gene ID" value="BRADI_3g58000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834410"/>
    <dbReference type="Gramene" id="KQK01754">
        <property type="protein sequence ID" value="KQK01754"/>
        <property type="gene ID" value="BRADI_3g58000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834410"/>
    <dbReference type="eggNOG" id="KOG4170">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105945_3_1_1"/>
    <dbReference type="OMA" id="IAPKENM"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1050.10:FF:000006">
        <property type="entry name" value="Non-specific lipid-transfer protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF27">
        <property type="entry name" value="OS02G0765800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="20"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK01754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="122" mass="13416" checksum="296F717BE499ACC4" modified="2012-06-13" version="1">MEESKLKAARLLLQMRDHLATDAGKELAEKVGFVYQLNISPKKLGVDEEIFVVDLKKGAVSKGPYEGKPDATFTFTDDDFLAISSGKLNPQMAFIRGKLKIKGSISAAQKFTPDIFPKPSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEX4</accession>
    <name>I1IEX4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100833409</name>
        <name evidence="2" type="ORF">BRADI_3g58160v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236086.1">
        <property type="nucleotide sequence ID" value="XM_010237784.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEX4"/>
    <dbReference type="EnsemblPlants" id="KQK01778">
        <property type="protein sequence ID" value="KQK01778"/>
        <property type="gene ID" value="BRADI_3g58160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833409"/>
    <dbReference type="Gramene" id="KQK01778">
        <property type="protein sequence ID" value="KQK01778"/>
        <property type="gene ID" value="BRADI_3g58160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833409"/>
    <dbReference type="eggNOG" id="ENOG502S5E7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160422_0_0_1"/>
    <dbReference type="OMA" id="AKCPPDK"/>
    <dbReference type="OrthoDB" id="1910345at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK01778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="156" mass="15932" checksum="CC8903AF7DC3434C" modified="2012-06-13" version="1">MSGGSGGERRGWSPMDAIRSFPSTPESLMSQIDAAIASTEYARACALLDPASSRPQPPATGSSNGGEASAARASPSPDPAAAPCHDARVADEAYRAACAALGAGRPDAAVRSLRAALASCPPEKAAAVAKVRSMLAIASAQLHRQQHQAQQLNSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1IG56</accession>
    <accession>A0A0Q3L023</accession>
    <name>I1IG56_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100826919</name>
        <name evidence="5" type="ORF">BRADI_4g00850v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85653.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236973.2">
        <property type="nucleotide sequence ID" value="XM_010238671.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IG56"/>
    <dbReference type="STRING" id="15368.I1IG56"/>
    <dbReference type="EnsemblPlants" id="KQJ85653">
        <property type="protein sequence ID" value="KQJ85653"/>
        <property type="gene ID" value="BRADI_4g00850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826919"/>
    <dbReference type="Gramene" id="KQJ85653">
        <property type="protein sequence ID" value="KQJ85653"/>
        <property type="gene ID" value="BRADI_4g00850v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_1"/>
    <dbReference type="OMA" id="CRVFASD"/>
    <dbReference type="OrthoDB" id="2102561at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000261">
        <property type="entry name" value="NADPH-dependent 1-acyldihydroxyacetone phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF5">
        <property type="entry name" value="ENOYL-(ACYL CARRIER) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ85653.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="313" mass="31915" checksum="FBC2745CF56E07C7" modified="2012-06-13" version="1">MATVNEDDDGGGKAKAAAAAGVGDDGAPVVLITGCAKGGIGYEYCVAFSALGCRVIATDIPAHIPDLTSDSTSAAAAVEVLPLDVTSDASVTAAVSQILATHGRIDVLVNNAGIGCTGPLAELGGESVRRAMDVNFHGQLRLIHAVAPHMAARRTGRIANVGSVVGTAATPWAGGYCASKAAVHAATDALRLELAPFGVRVVKVVPGAVRSGLGHANAAQMSAGAGDGGQKNRWGMYGDFAAAIEERATASQGKGTMEAAVFARHVARKVMSARPPREVVYGSMTVLFAVLAAAPVWARDRFFARRFGLNRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IGJ9</accession>
    <name>I1IGJ9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100846464</name>
        <name evidence="12" type="ORF">BRADI_4g02050v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579154.1">
        <property type="nucleotide sequence ID" value="XM_003579106.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IGJ9"/>
    <dbReference type="FunCoup" id="I1IGJ9">
        <property type="interactions" value="669"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IGJ9"/>
    <dbReference type="EnsemblPlants" id="KQJ85857">
        <property type="protein sequence ID" value="KQJ85857"/>
        <property type="gene ID" value="BRADI_4g02050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846464"/>
    <dbReference type="Gramene" id="KQJ85857">
        <property type="protein sequence ID" value="KQJ85857"/>
        <property type="gene ID" value="BRADI_4g02050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846464"/>
    <dbReference type="eggNOG" id="ENOG502RYH6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066104_1_0_1"/>
    <dbReference type="OMA" id="EMEFTSF"/>
    <dbReference type="OrthoDB" id="610574at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006459">
        <property type="entry name" value="CASP/CASPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044173">
        <property type="entry name" value="CASPL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01569">
        <property type="entry name" value="A_tha_TIGR01569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488:SF8">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="11">
        <location>
            <begin position="31"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ85857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="196" mass="20774" checksum="2A436A5CB985D04C" modified="2012-06-13" version="1">MDLEHGKKPSAHPAPCTVQQVKDKLIGLRPVMLRASALLATTVAAAVMGLNRQSYTAVVAIVGTKPLTQTFTAQFKDTPAFVYFVIANAIASLYNLVVLAMRRLVQGRVQRLVLHMGDMVIMLLLATGAATAASMAELGKNGNLHAHWNPMCDKFGSFCNRGGLSLVSSFIGVTLMLALNLLSAAANTPRGIVAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1II83</accession>
    <name>I1II83_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100823803</name>
        <name evidence="3" type="ORF">BRADI_4g06850v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576107.1">
        <property type="nucleotide sequence ID" value="XM_003576059.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1II83"/>
    <dbReference type="STRING" id="15368.I1II83"/>
    <dbReference type="EnsemblPlants" id="KQJ86643">
        <property type="protein sequence ID" value="KQJ86643"/>
        <property type="gene ID" value="BRADI_4g06850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823803"/>
    <dbReference type="Gramene" id="KQJ86643">
        <property type="protein sequence ID" value="KQJ86643"/>
        <property type="gene ID" value="BRADI_4g06850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823803"/>
    <dbReference type="eggNOG" id="ENOG502RZ2J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114289_0_0_1"/>
    <dbReference type="OMA" id="KWSKECN"/>
    <dbReference type="OrthoDB" id="1930465at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36080">
        <property type="entry name" value="DBJ|BAA96220.1"/>
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    </dbReference>
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        <property type="entry name" value="DBJ|BAA96220.1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ86643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="178" mass="19145" checksum="ED733C512F7B1947" modified="2012-06-13" version="1">MAQREKSAVAVASTATASPSSSSSDPPAAPSGERWGAAIGNLGELGANVESLQKLLARKAVFVDEDVFSKASLAAEQTRSIKILEQRVLSLERELDAAISAASRARTEKRQAEAAQRAAELRAQEVTKELENTARVFQLHMEELRAKQDEIAKRDSDIKVLEAIIRTLSSKDNGGSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJM8</accession>
    <name>I1IJM8_BRADI</name>
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        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g11060v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJM8"/>
    <dbReference type="EnsemblPlants" id="KQJ87428">
        <property type="protein sequence ID" value="KQJ87428"/>
        <property type="gene ID" value="BRADI_4g11060v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87428">
        <property type="protein sequence ID" value="KQJ87428"/>
        <property type="gene ID" value="BRADI_4g11060v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502STSX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053613_0_0_1"/>
    <dbReference type="InParanoid" id="I1IJM8"/>
    <dbReference type="OMA" id="VERYAFW"/>
    <dbReference type="OrthoDB" id="665134at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111">
        <property type="entry name" value="BNAA05G37150D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111:SF133">
        <property type="entry name" value="OS07G0196600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="3"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ87428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="401" mass="44864" checksum="2784160AF326B6C7" modified="2012-06-13" version="1">MDIAVDALVEILLLLPPSCRRRCRLVCRQWRDTVDRHTTEMQSRAKPLVVATGSAYIVDDLSSTGRSHRRLLWTDLVRDDHGRNAMTSVVGTCNGLICLYDSRSPGGAIAVVNPVTNETLHIPPLPQAVWVSRGHIVSWRGSYSFAYHPTTGRYTVVHVPCDYGKGCTDTFLQVFTLGEESPSSWRSVAVAPDEICTYLEAGVVSVDGATYWATTGDKKIVAFDFDGSDRVTSVDLLPNGDMLSRPGSWNLAEVRGRLGVAFYHPPSISESKIEVWVLEGATTEERRWSRWYNVLTHSWHGSDFNKMIGNWHLARPHFAQGEDILTQDLLWDSDGLRTLSVSKHRRTSKDTNGTAGCYMVEVSERNRAMVVGAMEAGYSWDRRIFSYVETTEPLSIYMSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQM7</accession>
    <name>I1IQM7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100839757</name>
        <name evidence="11" type="ORF">BRADI_4g31860v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578209.1">
        <property type="nucleotide sequence ID" value="XM_003578161.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQM7"/>
    <dbReference type="STRING" id="15368.I1IQM7"/>
    <dbReference type="EnsemblPlants" id="KQJ90482">
        <property type="protein sequence ID" value="KQJ90482"/>
        <property type="gene ID" value="BRADI_4g31860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839757"/>
    <dbReference type="Gramene" id="KQJ90482">
        <property type="protein sequence ID" value="KQJ90482"/>
        <property type="gene ID" value="BRADI_4g31860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839757"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005738_1_3_1"/>
    <dbReference type="OMA" id="WPPPPRQ"/>
    <dbReference type="OrthoDB" id="660550at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000029">
        <property type="entry name" value="Aspartyl protease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000016">
        <property type="entry name" value="Probable aspartic protease At2g35615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF38">
        <property type="entry name" value="OS09G0452400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095461" description="Peptidase A1 domain-containing protein" evidence="9">
        <location>
            <begin position="23"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="10">
        <location>
            <begin position="89"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ90482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="443" mass="47168" checksum="8BAEED6EF11834F0" modified="2012-06-13" version="1">MASIPAFLLVLLPLLLSAAVSAYPDAGFGFQATLTHIDAGAGYTEAQLLSRAVRRSKARVAALQSLATTTAADAITVARILVLASEGEYLMSMGIGTPPRYYSAILDTGSDLIWTQCAPCMLCVDQPTPFFDPAQSPSYAKLPCNSPMCNALYYPLCYRNVCVYQYFYGDSANTAGVLSNETFTFGTNDTRVTVPRIAFGCGNLNAGSLFNGSGMVGFGRGPLSLVSQLGSPRFSYCLTSFMSPVPSRLYFGAYATLNSTSASTGEPVQSTPFIVNPGLPTMYYLNMTGISVGGELLPIDPSVFAINDADGTGGVIIDSGSTITYLARAAYDMVHQAFADQVGLPLTNATSLADVLDTCFVWPPPPRKIVTMPELAFHFEGANMELPLENYMLIDGDTGNLCLAIAASDDGSIIGSFQHQNFHVLYDNENSLLSFTPATCNVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1IRD7</accession>
    <name>I1IRD7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">MBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100832702</name>
        <name evidence="12" type="ORF">BRADI_4g34050v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578356.1">
        <property type="nucleotide sequence ID" value="XM_003578308.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IRD7"/>
    <dbReference type="STRING" id="15368.I1IRD7"/>
    <dbReference type="EnsemblPlants" id="KQJ90818">
        <property type="protein sequence ID" value="KQJ90818"/>
        <property type="gene ID" value="BRADI_4g34050v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT64856">
        <property type="protein sequence ID" value="PNT64856"/>
        <property type="gene ID" value="BRADI_4g34050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832702"/>
    <dbReference type="Gramene" id="KQJ90818">
        <property type="protein sequence ID" value="KQJ90818"/>
        <property type="gene ID" value="BRADI_4g34050v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64856">
        <property type="protein sequence ID" value="PNT64856"/>
        <property type="gene ID" value="BRADI_4g34050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832702"/>
    <dbReference type="eggNOG" id="KOG4161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="PICATHL"/>
    <dbReference type="OrthoDB" id="10072024at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IRD7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01396">
        <property type="entry name" value="MeCP2_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.890.10:FF:000012">
        <property type="entry name" value="Methyl-CpG-binding domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011124">
        <property type="entry name" value="Znf_CW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396:SF0">
        <property type="entry name" value="METHYL-CPG BINDING DOMAIN PROTEIN-LIKE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396">
        <property type="entry name" value="METHYL-CPG BINDING PROTEIN, MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07496">
        <property type="entry name" value="zf-CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51050">
        <property type="entry name" value="ZF_CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CW-type" evidence="11">
        <location>
            <begin position="75"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="MBD" evidence="10">
        <location>
            <begin position="140"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="224"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT64856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="35241" checksum="51AA0FE5815E2DA3" modified="2012-06-13" version="1">MDSSKSTQALKKSQTMLSGTDGHQFDNDEFTSEGALDKMVVSKSETSDKGQDEFGENHSPSLQQSTPSNTPNRGSPCIGAFTIQCAKCFKWRLVPTKEKYEEIREHIIQEPFECERAREWKPDVTCDDPEDISQDGSRLWAIDKPNIARAPNGWERQIRIRGEGGTKFADVYYTSPSGRKLRSQVEVDKYLKENPEYGEKGVKSTQFSFQIPRPLRENYVKHVKKRPKLASPSDEANIITSKPLQPVEANPISWAAPPTHEGQASEQVSHAADPFGSVEAELTRKRKAESSPGEVNASDELKPKIEDAQNGDTSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1IS74</accession>
    <name>I1IS74_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase</fullName>
            <ecNumber evidence="5">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100827215</name>
        <name evidence="9" type="ORF">BRADI_4g36230v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="5"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578507.1">
        <property type="nucleotide sequence ID" value="XM_003578459.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IS74"/>
    <dbReference type="FunCoup" id="I1IS74">
        <property type="interactions" value="2074"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IS74"/>
    <dbReference type="EnsemblPlants" id="KQJ91203">
        <property type="protein sequence ID" value="KQJ91203"/>
        <property type="gene ID" value="BRADI_4g36230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827215"/>
    <dbReference type="Gramene" id="KQJ91203">
        <property type="protein sequence ID" value="KQJ91203"/>
        <property type="gene ID" value="BRADI_4g36230v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827215"/>
    <dbReference type="eggNOG" id="KOG1532">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037460_1_1_1"/>
    <dbReference type="OMA" id="MIIVFNK"/>
    <dbReference type="OrthoDB" id="243313at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000817">
        <property type="entry name" value="GPN-loop GTPase 1"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="Gpn"/>
    </dbReference>
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        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="63"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
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        <location>
            <begin position="361"/>
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        </location>
    </feature>
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        <location>
            <begin position="312"/>
            <end position="351"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="22"/>
            <end position="43"/>
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        <location>
            <begin position="370"/>
            <end position="381"/>
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            <begin position="388"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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        <source>
            <dbReference type="RuleBase" id="RU365059"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ91203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="401" mass="44528" checksum="FFF50597AC921E06" modified="2012-06-13" version="1">MDVDSEPSAESKPTQMDLEDQTDVKGKGKADATEEEGNGKGEELADSIGSLSIGPGRTNFKKKPVIILVIGMAGTGKTTFMHRLVCHTQASSNMRGYVINLDPAVMTLPFGANIDIRDTVRYKEVMKEYGLGPNGGILTSLNLFSTKFDEVISVIEKRADQLDYVLVDTPGQIEIFTWSASGAIITEAFASTFPTVIAYVVDTPRSTSPVTFMSNMMYACSILYKTRLPLVLTFNKVDVAKHEFALEWMQDFEAFQTALDSDSSYSSSFTRSLSLVLDEFYNNLRSVGVSAVSGTGVDAFFEAIEASANEYMENYRADLDKRIAEKERLEAERRNENMERLRRDMEKSKGQTVVLSTGLKDKIPASELMGDADEEEQEEALEDFRISDDEDEDEDVDDDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITW0</accession>
    <name>I1ITW0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS7c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100821763</name>
        <name evidence="7" type="ORF">BRADI_4g41050v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQJ91993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003578781.4"/>
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    <dbReference type="FunCoup" id="I1ITW0">
        <property type="interactions" value="2280"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1ITW0"/>
    <dbReference type="EnsemblPlants" id="KQJ91993">
        <property type="protein sequence ID" value="KQJ91993"/>
        <property type="gene ID" value="BRADI_4g41050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821763"/>
    <dbReference type="Gramene" id="KQJ91993">
        <property type="protein sequence ID" value="KQJ91993"/>
        <property type="gene ID" value="BRADI_4g41050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821763"/>
    <dbReference type="eggNOG" id="KOG3291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063975_0_0_1"/>
    <dbReference type="OMA" id="KMNIVER"/>
    <dbReference type="OrthoDB" id="10264639at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
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    </dbReference>
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        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="entry name" value="40S ribosomal protein S5"/>
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        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribosomal_uS7"/>
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    </dbReference>
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        <property type="entry name" value="Ribosomal_uS7_dom"/>
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    </dbReference>
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        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
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        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal protein S7"/>
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    </dbReference>
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        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="6">
        <location>
            <begin position="40"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ91993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="199" mass="22164" checksum="2913EC80423A0A38" modified="2012-06-13" version="1">MAAVEVPTNDVKLFNRWSFDGVEVQDISLNDYIGVNPDKHATYLPHTAGRYSAKRFRKAQCPIIERLTNSLMMHGRNNGKKLMAVRIIQHTMEIIHLLTDANPIQVIVDAIINSGPREDATRIGSAGVVRRQAVDISPLRRVNQAIYLLTTGARESAFRNVKTIAECLADELINAAKGSSNSYAIKKKDEIERVAKANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I1IW95</accession>
    <name>I1IW95_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF629 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC104585508</name>
        <name evidence="2" type="ORF">BRADI_5g03436v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311988.1">
        <property type="nucleotide sequence ID" value="XM_024456220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IW95"/>
    <dbReference type="EnsemblPlants" id="PNT60697">
        <property type="protein sequence ID" value="PNT60697"/>
        <property type="gene ID" value="BRADI_5g03436v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104585508"/>
    <dbReference type="Gramene" id="PNT60697">
        <property type="protein sequence ID" value="PNT60697"/>
        <property type="gene ID" value="BRADI_5g03436v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3ZV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_467248_0_0_1"/>
    <dbReference type="OMA" id="RETICTS"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34465">
        <property type="entry name" value="CARBOXYL-TERMINAL HYDROLASE-LIKE PROTEIN, PUTATIVE (DUF627 AND DUF629)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34465:SF6">
        <property type="entry name" value="OS11G0599000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT60697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="63476" checksum="E5091C151952972E" modified="2012-06-13" version="1">MADTNSSAVVAAEAAESSHGGRGGRAQRRRRAGNDRSMTEEHVSAQPGSSLVAAGQAVADGASVSTTREACREALRRYHGAAIALADELARQNPGYTVAINLAAGLHQAAGSEARDLVIRRGDKESVAGEKEAVAVAARHFSSAILGYNRVVRLASRFITTASARAKVLAASQKFDLAHEEFLRAMAISVPDDPSDHNVGYDLRRRWTSGELHRSTTSMRKDRAKAELQKQVRAFRETICTSYIPRQATFVWEAFKSGSGEPDAISEARDQAEHLVYYYPFCPRAHLLFGKLELEMVRNSDGDGELESVRNSDGVGASRQVSALGQVLKMMSKAASAFPKSGVISLVRAEVCYLLKEYDATARECCRALQIRRPDDPGLQDIPIGSTTGSDPDARINTIRILLQELLGKVTSETPTTPDGVDSPAMSFSKDSAPPVSAKPGSETTVSAQDTHALSGNEDLEEPAMVADLIMTAKKQTLPIHFARGRYIDEGVVNLYKEYCIALSQCSEKRTVCHLCCFEGVYCPKILKGNMNVHFHNEHWKGKETVNCDEKIATPASELMLIFVCITTLYIGFSQAGGRSHSYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1IWD0</accession>
    <name>I1IWD0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100844339</name>
        <name evidence="1" type="ORF">BRADI_5g03697v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581306.1">
        <property type="nucleotide sequence ID" value="XM_003581258.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IWD0"/>
    <dbReference type="STRING" id="15368.I1IWD0"/>
    <dbReference type="EnsemblPlants" id="KQJ81900">
        <property type="protein sequence ID" value="KQJ81900"/>
        <property type="gene ID" value="BRADI_5g03697v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ81901">
        <property type="protein sequence ID" value="KQJ81901"/>
        <property type="gene ID" value="BRADI_5g03697v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844339"/>
    <dbReference type="Gramene" id="KQJ81900">
        <property type="protein sequence ID" value="KQJ81900"/>
        <property type="gene ID" value="BRADI_5g03697v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81901">
        <property type="protein sequence ID" value="KQJ81901"/>
        <property type="gene ID" value="BRADI_5g03697v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844339"/>
    <dbReference type="eggNOG" id="ENOG502QSKC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_2_1_1"/>
    <dbReference type="OMA" id="ESEFQGC"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IWD0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47821">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47821:SF2">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ81900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="234" mass="25632" checksum="78D1A5A6B59E58D7" modified="2012-06-13" version="1">MEDSSCNSPSPPIFRNRYWILRHGRSVPNDRGLIVSSLENGTKQEFGLAPLGVQQARSAGESLRKELEEMGVPVDSVKIHYSPFSRTTETARVVAGVLGVPFEGPNCKAVVELRERYFGPSYELLSHEKYAEVWAVDEADPYIAPEGGESVADVASRFAAMLSSADMEFHGSAVLIVSHGDPLQIFQAVLSGTKKITSFLEEVSDLQKKSLIAPSLLSGHRKFALNTGELRRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IXE5</accession>
    <name>I1IXE5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Arogenate dehydratase</fullName>
            <ecNumber evidence="3 11">4.2.1.91</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100829551</name>
        <name evidence="14" type="ORF">BRADI_5g09030v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Converts the prephenate produced from the shikimate-chorismate pathway into phenylalanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arogenate + H(+) = L-phenylalanine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:12536"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:58180"/>
            <dbReference type="EC" id="4.2.1.91"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-phenylalanine from L-arogenate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Plastid</location>
            <location evidence="1 11">Chloroplast stroma</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.2.1.91" evidence="3 11"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579674.1">
        <property type="nucleotide sequence ID" value="XM_003579626.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IXE5"/>
    <dbReference type="FunCoup" id="I1IXE5">
        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IXE5"/>
    <dbReference type="EnsemblPlants" id="KQJ82451">
        <property type="protein sequence ID" value="KQJ82451"/>
        <property type="gene ID" value="BRADI_5g09030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829551"/>
    <dbReference type="Gramene" id="KQJ82451">
        <property type="protein sequence ID" value="KQJ82451"/>
        <property type="gene ID" value="BRADI_5g09030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829551"/>
    <dbReference type="eggNOG" id="KOG2797">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035008_4_1_1"/>
    <dbReference type="OMA" id="TNLNIVP"/>
    <dbReference type="OrthoDB" id="2414662at2759"/>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00344"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047769">
        <property type="term" value="F:arogenate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13631">
        <property type="entry name" value="PBP2_Ct-PDT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000019">
        <property type="entry name" value="Arogenate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000028">
        <property type="entry name" value="Arogenate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000031">
        <property type="entry name" value="Arogenate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF41">
        <property type="entry name" value="AROGENATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022">
        <property type="entry name" value="PREPHENATE DEHYDRATASE P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00857">
        <property type="entry name" value="PREPHENATE_DEHYDR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00858">
        <property type="entry name" value="PREPHENATE_DEHYDR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 11" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 11" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="12">
        <location>
            <begin position="115"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="13">
        <location>
            <begin position="307"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ82451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="419" mass="44930" checksum="673155060E693E8E" modified="2012-06-13" version="1">MAAASLIRAPVGRLPCHAPGRSGGVVRCSLQGAVVGGRTEWLSNCAVLSSKVAALGPHSVNGHAAPPMAPAPTNGPVLDLIPVSSINGGAKNNNLPVPLKISDLSPAPMHGSELRVAYQGVPGAYSEKAAGKAYPGCDAIPCDQFEVAFQAVELWIADRAVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVRKENITRVISHPQALAQCEHTITRMGLNVVREAFDDTAGAAEYVAAHGLRDTAAIASSRAAELYGMEVLADGIQDDCGNVTRFVMLAREPIVPRTDRPFKTSIVFAHDKEGTSVLFKVLSAFAFRDITLTKIESRPHRHRPIRLVDDANRGTAKHFEYMFYVDFQASLAEPRAQNALAEVQEFTSFLRVLGSYPMDMTPMTAGSSSTVTSSDPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I1J1M6</accession>
    <name>I1J1M6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_5g21270v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J1M6"/>
    <dbReference type="STRING" id="15368.I1J1M6"/>
    <dbReference type="EnsemblPlants" id="KQJ84499">
        <property type="protein sequence ID" value="KQJ84499"/>
        <property type="gene ID" value="BRADI_5g21270v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ84499">
        <property type="protein sequence ID" value="KQJ84499"/>
        <property type="gene ID" value="BRADI_5g21270v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ2H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015477_0_3_1"/>
    <dbReference type="InParanoid" id="I1J1M6"/>
    <dbReference type="OMA" id="IYIEMAL"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF48">
        <property type="entry name" value="METAL-NICOTIANAMINE TRANSPORTER YSL17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ84499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="141" mass="15142" checksum="B519170E5A6E502F" modified="2012-06-13" version="1">MGPYAKVYRGISLLGMTTENGLPTHTMLLCKVFFALALSLGVLREAATHRGWRRVRRCVPSTIGMAVAFFVPPTIPVGMFVGSVVIYLCGSADDDGMRMMSPAVACGLICGDGFGSLLSSMLTLLKARPPICIKFVSRPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1J3C0</accession>
    <name>I1J3C0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100838244</name>
        <name evidence="3" type="ORF">BRADI_5g26090v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240608.1">
        <property type="nucleotide sequence ID" value="XM_010242306.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J3C0"/>
    <dbReference type="EnsemblPlants" id="KQJ85277">
        <property type="protein sequence ID" value="KQJ85277"/>
        <property type="gene ID" value="BRADI_5g26090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838244"/>
    <dbReference type="Gramene" id="KQJ85277">
        <property type="protein sequence ID" value="KQJ85277"/>
        <property type="gene ID" value="BRADI_5g26090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838244"/>
    <dbReference type="eggNOG" id="ENOG502SNVW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065685_0_0_1"/>
    <dbReference type="OMA" id="WPVAQHT"/>
    <dbReference type="OrthoDB" id="778097at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ85277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="33511" checksum="637C7DE4DF0861D8" modified="2012-06-13" version="1">MSGSYASRLRSGGGGGVGAGGGLGTTTVLAAKVVFASAAVAAAASLARGLAVPQLVVSVAAGADAFLWKLYLFVAVHVIIFVIWKLSDGHKHFHHKDPWVDDDGGVKRKEDFVSVSGADAIQAGLARRKDSWAAPAARGPPPPPVVSGFIPKEEFCSTAGYGVPPLQKKFSPMEEVVSPDSGACESCVTTTEYEEADDSSSPAAAADEWRSVAPAPLQRADLSLPLPPPAMAAAAMDHCFDANAAADDGGDDDLDATWNAIMQKKRPSTAPASSTPPAPQAPAPPPAAAVAARPRAREPSVGAAELSKRADDFIKKIHNSFGRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E6I4</accession>
    <name>A0A0Q3E6I4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_5g03246v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E6I4"/>
    <dbReference type="STRING" id="15368.A0A0Q3E6I4"/>
    <dbReference type="EnsemblPlants" id="KQJ81812">
        <property type="protein sequence ID" value="KQJ81812"/>
        <property type="gene ID" value="BRADI_5g03246v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81812">
        <property type="protein sequence ID" value="KQJ81812"/>
        <property type="gene ID" value="BRADI_5g03246v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E6I4"/>
    <dbReference type="OrthoDB" id="620200at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF50">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN ITN1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="130"/>
            <end position="163"/>
        </location>
    </feature>
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        <location>
            <begin position="226"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ81812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="419" mass="46702" checksum="C67D9C7B40CAD6EA" modified="2016-01-20" version="1">MDSLTRKLLKWNNDLTTQRDENGSTPLHFAAALMRQSQQGSVCWQVLEANPAALYQSDHNGLFPIHVAASVGERGTITMFLSKSPSSAGLQDTKRRTFLHVAAEKKKVRIVHYACRNQSLSWILNMQDNDGNTALHLAIQAGSLIMFCALLGNRHVHLNLTNKKGQTPLDVSRYKIPPGLFDDENSERKIHFALTVTKARSGGCRQDHFEENYSHQLKHNEREETEKVKESTHTLCIGTVLIATVTFGAIFALPGGYRVEDHDNGGTPTLPGRYAFDGFIIASTFAFILSAMATVSLMRSGYSISNPYSRRIYLILALYLGSTSITCFITAFALGIYMVLAPVARETALAICVISSLVVVCNKMEFWLKWALLARPLCARIGLIRTAVMVSTSILLNLFMEFWPMILTFVWAAYARNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FCE9</accession>
    <name>A0A0Q3FCE9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g27822v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FCE9"/>
    <dbReference type="EnsemblPlants" id="KQJ96926">
        <property type="protein sequence ID" value="KQJ96926"/>
        <property type="gene ID" value="BRADI_3g27822v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96926">
        <property type="protein sequence ID" value="KQJ96926"/>
        <property type="gene ID" value="BRADI_3g27822v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FCE9"/>
    <dbReference type="OrthoDB" id="632669at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ96926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="115" mass="13028" checksum="4D0A1BBA461A25D3" modified="2016-01-20" version="1">MYTQRVFKTTVDEDIPSSPRSSSSPHEESPLKVETSTKKTYLGPMTRSRAKQIQQEVNSLLAAPNDNINENFILPNSRVLLVLRFTTRSQTCCPDHQTTHEQLQNSSPTLLQHCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FNR2</accession>
    <name>A0A0Q3FNR2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BRADI_3g45281v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the UQCRH/QCR6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FNR2"/>
    <dbReference type="FunCoup" id="A0A0Q3FNR2">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FNR2"/>
    <dbReference type="EnsemblPlants" id="KQJ99804">
        <property type="protein sequence ID" value="KQJ99804"/>
        <property type="gene ID" value="BRADI_3g45281v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99804">
        <property type="protein sequence ID" value="KQJ99804"/>
        <property type="gene ID" value="BRADI_3g45281v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FNR2"/>
    <dbReference type="OrthoDB" id="405848at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FNR2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.20:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.20">
        <property type="entry name" value="Ubiquinol-cytochrome C reductase hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003422">
        <property type="entry name" value="Cyt_b-c1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023184">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336:SF1">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.8 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02320">
        <property type="entry name" value="UCR_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000019">
        <property type="entry name" value="Bc1_11K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome C reductase hinge" evidence="12">
        <location>
            <begin position="8"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ99804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="69" mass="8201" checksum="8938C90B54FE62C3" modified="2016-01-20" version="1">MSDEEVSDPKALLEDRSKAKCVSQWYEYQKCVKRIEDDETGQKHCTGQYFDYWKCIDKNVAEKLFDSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FX42</accession>
    <name>A0A0Q3FX42_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100824494</name>
        <name evidence="9" type="ORF">BRADI_2g11240v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567564.1">
        <property type="nucleotide sequence ID" value="XM_003567516.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FX42"/>
    <dbReference type="STRING" id="15368.A0A0Q3FX42"/>
    <dbReference type="EnsemblPlants" id="KQK04023">
        <property type="protein sequence ID" value="KQK04023"/>
        <property type="gene ID" value="BRADI_2g11240v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK04024">
        <property type="protein sequence ID" value="KQK04024"/>
        <property type="gene ID" value="BRADI_2g11240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824494"/>
    <dbReference type="Gramene" id="KQK04023">
        <property type="protein sequence ID" value="KQK04023"/>
        <property type="gene ID" value="BRADI_2g11240v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04024">
        <property type="protein sequence ID" value="KQK04024"/>
        <property type="gene ID" value="BRADI_2g11240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824494"/>
    <dbReference type="OrthoDB" id="514967at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FX42">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF204">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26102">
        <property type="entry name" value="Ig_SPL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="8">
        <location>
            <begin position="129"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="785"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="807"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="887" mass="98503" checksum="14B594C645DFD107" modified="2016-01-20" version="1">MEAGFRREGGQTGLANTMSSELSKNKKKKGLEWDLNDWRWDGNLFLATPSSNAVAAPSGCGSRELGRSEDGIDLSVADKRRRVSPVDNHGECSNAAIANGDHDRVVGRRGQSSREGRHANATGAYSTSAPYCQVDGCHADLRDSRDYHKRHKVCEVHTKSTVVRIKSIEHRFCQQCSRFHLLPEFDEGKKSCRSRLAKHNGRRRKAPAQAGAAGNTSSENQSLTNTLLLLLKQLSGQDPGSSSEQSNGPNFLVNLLKNLAAIAGTQAYQDMLKNANSASISSNDANYVVNGFTNEPTRSPIPVGTESSAEHTVKQHVQNFDLNDAYVEEDESRTDKIVFKLFGKEPKDFPVDLRAQMLNWLSHYPSDMESHIRPGCVILTIYLRLPNWMWDKLKVNPAPWIENLISISTDGFWETGWLYTRLQDRLALSCNGRLMLVSPWQPLIGDKHQILCVTPIATACSSTANFSVKGFNIVQPTTKLLCIFGGEYLIQEATQMLHEDTMMQQGPQCLTFSCSFPSMSGRGFIEVEDYDQSSLSFPIVVAEESLCSEIRMLEDKLNLIAFGDILEGREDLMASRDQALNFLQEIGWFLQRSHKRATTDAPQYCTESFPVARFRWLLSFAIDQEWCSVVKKLLDTLFQGNIDVDVRSPFEFVLGEGLVFTAVNKRAKRLVEFLLRYTTNSALVARGAVSPVRFLFTPDITGSSNITPLHIAASMSDGAGVLDALTDDPQQLGIKAWKHARDTTGYTPEDYAQKRGHISYIQMVENKINSRLPKAHVSVSMTISPSTTDISEKHAGRSKSTNQTTLDIEKSQRSDKRPPSCRQCVQLQHIAYHPRPNRFLSNRPAVLSLVAIGAVCVCVGLIMQSPPTVGVGMTGPFLWSSLNYGPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FYG2</accession>
    <name>A0A0Q3FYG2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100832633</name>
        <name evidence="4" type="ORF">BRADI_2g07460v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03373.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565513.2">
        <property type="nucleotide sequence ID" value="XM_003565465.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FYG2"/>
    <dbReference type="EnsemblPlants" id="KQK03373">
        <property type="protein sequence ID" value="KQK03373"/>
        <property type="gene ID" value="BRADI_2g07460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832633"/>
    <dbReference type="Gramene" id="KQK03373">
        <property type="protein sequence ID" value="KQK03373"/>
        <property type="gene ID" value="BRADI_2g07460v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="202415at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF103">
        <property type="entry name" value="GLYCOSYL TRANSFERASE CAP10 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CAP10"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="3">
        <location>
            <begin position="225"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK03373.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="551" mass="63236" checksum="87FD954F40AB9C79" modified="2018-03-28" version="2">MKSSLPDEEEMAAMVPAAVERQSSPTRTTTKSWWKLGSGVGMIIGSLVLLALLFGTKRIHLDAAYYNNSFLPATDHSTGSSGFSRRGRPPHPRRAHSPLVPIPFTCGNDTSPSSPASLCRRRRRAPSPAPAPSSSQGARRWRFDPSVAPPPWCPVYFRHIHTDLDPWRSTGITRDTLERAMPHAEFRLTVVSGRAYVQNLRPSYQTRDVFTQWGVLQLLARFPGRVPDVDIMFSAGDVAQVLSADYYNTTTHPPPPLFRYCKEEKLEAAIVFPDWSFWGWPELSIRPWAPLMEDFVRENKALPWRNRQPYAFWKGNPEVSDVRRDLFKCNNDSANGKDWNARLFWQDWNAASRNGFRDSNLAKQCDYRYKIYVQGHAWSVSEKYILACDSPMLAVDTSYVDFFSRGLVAGRHYWPIDPARKCRAVKFAVGWGNEHAGQAMRMGQEGSRFAREEMSMDYVYEYMFHVITEYAALLRYRPTVPEKAVEVCVESLACGRRGREKEFLMESREEYEARYEPCTLPPPFTDEEAREMAAWDREVRSKLVKMEEEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G1J3</accession>
    <name>A0A0Q3G1J3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g19221v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK05288"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
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        <name type="common">Purple false brome</name>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_2g55850v3"/>
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    <dbReference type="OrthoDB" id="693957at2759"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="174"/>
        </location>
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            <begin position="1"/>
            <end position="15"/>
        </location>
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            <end position="42"/>
        </location>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK10736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GSG9</accession>
    <name>A0A0Q3GSG9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100829043</name>
        <name evidence="5" type="ORF">BRADI_1g12490v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Brachypodium</taxon>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="RefSeq" id="XP_014752309.1">
        <property type="nucleotide sequence ID" value="XM_014896823.2"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GSG9"/>
    <dbReference type="EnsemblPlants" id="KQK13784">
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        <property type="gene ID" value="BRADI_1g12490v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13785">
        <property type="protein sequence ID" value="KQK13785"/>
        <property type="gene ID" value="BRADI_1g12490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829043"/>
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        <property type="protein sequence ID" value="KQK13784"/>
        <property type="gene ID" value="BRADI_1g12490v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13785">
        <property type="protein sequence ID" value="KQK13785"/>
        <property type="gene ID" value="BRADI_1g12490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829043"/>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GSG9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
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    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF6">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="4">
        <location>
            <begin position="1138"/>
            <end position="1288"/>
        </location>
    </feature>
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        <location>
            <begin position="2353"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2464"/>
            <end position="2484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="2600"/>
        </location>
    </feature>
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        <location>
            <begin position="1984"/>
            <end position="2011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2568"/>
            <end position="2585"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK13784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="2600" mass="291603" checksum="AD93154338CF2FD4" modified="2016-01-20" version="1">MASSPVKFFSVFLAVSVIGWVVFTFAARLLAWFLSRVLRASVAFRVAGFNCLRDVTIKFSKGSLESISIGEIKLSFRKSLVKLSFGVISKDPKVQLLINDLEIVTRSSSQSKKSSKSTRPRSTGKGKWLVTSSMARLLSVSVADLMIKVPDGAVDIKELKVDTFKIAGPNHILGVKLLLLPLNVHLGDFGFTADPVGNCNQLDAFQSDQASLASSEKFLAPFVFEDLLVTCEFGHEKERGVKIVNLELECGDVSANIDERLFHKKHTKLGNNSLSENAEEAIPGTSPTKLSSKSKSILPALKKQMLAFPDKVSFSVPKLDVKFTHLGEGLTVHNNVMGIHCTSVKSLPQDDLEEATPHFDVQVDLSEIHLVREGSSSLLEVLKVAAVASLDIPLDPFLPIRAEIDAKLGGTQCNLMLSRLMPWMRLHSLKTKGMKLSKENSHLEVSQTKEIKQIMWTCTVSAPEMTIMLYSSSGLVLYHVCCQSSHLFANNIASKGIQIHTELGEMLVHMEDGYREFLKENIFGVDTYSGSLMHIARVSLDWGYREIEVQDMAETSRLALVFSVDISGIGVKFSFKHLESLVLNLMSFRTLLKDLSSSRERAKENNLEHRGKKKTKGVEILKLSLQKFSITYSGDVNILNMPIADPKRVNYGSQGGQVIVNVSADGTPRRATIASEQPGCGRNLRFSASLVISHLSVCIDKEKKSTQAELERVKTIYEEDHSSGGKVTLLDMQNAKIVRRSGDLTGVAVCSLFSATDINIRWEPDAHLALFETLVRFKYFLHHNKFQSSEKLSNTQNGNIKEKSDKRGSIFAVDVEVLRISAGLADGVEANMQVQSIFTENARIGVLSEGLSLSLNGARVLKSTRIQISCIPFATGHLPNAKVDPSSKRDWVIQGLDVHVCMPYRLPLRGIEDAVEDTIRALKLVSAAKRSIVFPDGKENLKKVKPGTSSFGSVKFVLRKLTAEIEEEPIQGWLDEHYHLMRNKTCESGVRLKFLDDAISGIVDPNHCSSEGKVLYDGIEVDLHDTAALQRMREEIHKKAFRSYYLACQKMVFAEGSGACTEGFQAGFKPSSRRASLLSLSASELDITLTRIEGGETGMVEFIKELDPVCQEKDIPFSRLYGSDIAVLAGSLVIQVRDYTSPLFSATSGKCQGRVVLAQQATCFQPQIHQDVYIGRWHKVTMLRSASGTTPAIKLYSNLPVYFQRGEISFGVGYEPSFADISYAFQVALRRVNLSTRDRNSGLANQPPKKERSLPWWDDVRYYIHGKIILYFSETKWSLLATTNPYEKVDRLQIVSEYMEIQQTDGHVDVSAKEFRMYISSLESMMKNCSLKVPSGVPRPFIYAPLFSLSVIIDWQCESGNPLNHYLHALPIEGEPRKKVYDPFRSTYLSLRWNFSLRPLQVQSDNAASASCYANSPMLSGTISGNCSTVADVDFPTMNLGAHDLAWVFKWWSLNYSPPHKLRSFSRWPRYKIPRAARSGNLSMDKVLVEFFFRVDATPCCIRHATLTEDDPASGLTFKMSRLKYELCYSRGKQKYTFDCKRESLDLVYRGLDLYKPEVYLVRDVNLSSAENVSKLKTSSHQSLGKIANDKCSMGNFQEKHEEGFLLSSDYFTIRRQAPKADRARLTEWRQDAGRNIEIAYVGSEFENGGESDHPLSEPSDDDDGFNWMLADNCQRVFVYSLRLLWTIENRDAVWSWVGGISKAFEPPKPSPSRQYAQRKMIEERQNADGSRLTQDAISSIHVGSPSVQHAEALSSTSPLHMKYGMFDDSDKGGNLHFMVNVVKPQFNLHSEEANGRFLLAAASGRVIARSFHSVVHVGKEMLEQALGTSSLHIPELQPEMTWKKADLSVWLEDVQAHVAPTDVDPGAGLQWLPRILGSSEKLKRTGALLERVFMPCQMYFSYTRHKGGTADLKVKPLKELRFNSPNITATMTSRQFQVMLDVLSNLLFARLPKPRKNSLQYPSDDEDIEEEADEVVPDGVEEVELAKINLEQKERERKLLLDDIRSLTGSSDYHMDLSAEKDNSLWMINSAKTSLVEGLKRDLLNLQKSRKFASSALRKALQKAAQLRLMEKEKNKTPSCAMRISMKISKVVWSMLADGNTFAEAEISDMGYDFDRDYKDIGVARFTTKYFVVRNCMANAKCDTLLSAWNAPPEKGAMLRVDAKQGAPKDGNSPLELFQVEIYPLKIYLPEAMYRMMWDYFFPEEDDSQRRQDIWRVSTSTGSRRTRRISSGAEAVASTSYSVREHELPGRSGTTISTSTNVSSWQGIHGDNSQVSKLQSLKANMVCGSHPELRRTSSFERTWEESAAENITSNDVVSLLNSSNVSSKGDTINSTAENTVAAAEMFRTKTKDSKSIKSGRLSHEEKKIGKSHDEKRTRARKLMEFHNIKISQVELLVTYEGSRLAINDLRLLMDTFHRVEFTGTWRRLFSRVKKHIIWGVLKSVTGMQGKKFKAQNQRETLPENDLNFSDSDGSHHGKPDQFPVSWLKRPGDSAGDGFVTSIRGLFNSQRRKAKAFVVRAMRGDGDNEYHDEWSDSDGEYPFARQLTVTKAKKLFRKKFRQRGQKSIGPSLQDSLPSSPRETTPYQSDSSESSYEDFHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GZU7</accession>
    <name>A0A0Q3GZU7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g05973v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GZU7"/>
    <dbReference type="EnsemblPlants" id="KQJ86506">
        <property type="protein sequence ID" value="KQJ86506"/>
        <property type="gene ID" value="BRADI_4g05973v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ86506">
        <property type="protein sequence ID" value="KQJ86506"/>
        <property type="gene ID" value="BRADI_4g05973v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GZU7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ86506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="79" mass="8988" checksum="DA652CEE7716AE36" modified="2016-01-20" version="1">MSGISIQIQRSSIMFIIVISFNILTNFEMLSISNLHYNSCTTTSLLCLNLIVLFLKIAACPSFEPSYLRTNHHVDQEFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H615</accession>
    <name>A0A0Q3H615_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">At1g61320/AtMIF1 LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100833336</name>
        <name evidence="3" type="ORF">BRADI_1g42250v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560761.1">
        <property type="nucleotide sequence ID" value="XM_003560713.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H615"/>
    <dbReference type="STRING" id="15368.A0A0Q3H615"/>
    <dbReference type="EnsemblPlants" id="KQK18392">
        <property type="protein sequence ID" value="KQK18392"/>
        <property type="gene ID" value="BRADI_1g42250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833336"/>
    <dbReference type="Gramene" id="KQK18392">
        <property type="protein sequence ID" value="KQK18392"/>
        <property type="gene ID" value="BRADI_1g42250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833336"/>
    <dbReference type="OrthoDB" id="617794at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H615">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF34">
        <property type="entry name" value="OS05G0571700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="At1g61320/AtMIF1 LRR" evidence="2">
        <location>
            <begin position="184"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK18392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="588" mass="66639" checksum="F6F6152DA25B59A4" modified="2016-01-20" version="1">MGLLKLNQLMSMKRRRRRNRRRQIQTRNGSIASCAKSKGRHDDEHSLDGQIKGYSISDLPEVETLYIVLNKLSTITFQLSDVSNSVYFVHSGDSVQFYCFPYRNNVTVFAFTIPLLIFLPHMCMQDILCRIHSLMPIQDAARAACVSQTFLHSWRCRPNLDFSRETLGLTKETQRDITSIVDHILQNHSGVGVKAVKFLDDSWLWNSIKNQDFRHLDVENQDFSHLDLDRWLRNTIKPGIEELNISLHGENTVYNFPCSLLSDEIGESLRNLKLVGCYFDPTIGLGSLRNLRRIQLGSVSITDSKLECLLSNSFSLEQLVLRVCSGIICLKIPCLQRLSYLEVNACTGLEVLESKAPNLSSVIIQEAPHVQLSLLESPRITKYYRSCPGAAFYARTELPSSMPNLETLSLVSNTETVNTPVMPSKFLHLKWLSISLSGRGQTYDLFSLSSFFDASPFLETFKLNASLHVKRGSIFEDRSDLRKMPEKHSYKLKCVRITNFSSAKSLIELTCHILESAMSLERLTLDTTHGAPRCSVMKTVRCWPMKKDALMEAHRALSAIQTYVKSKVPSTVELNVFEPCSQCHAVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HCM5</accession>
    <name>A0A0Q3HCM5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g55796v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HCM5"/>
    <dbReference type="EnsemblPlants" id="KQK20647">
        <property type="protein sequence ID" value="KQK20647"/>
        <property type="gene ID" value="BRADI_1g55796v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20647">
        <property type="protein sequence ID" value="KQK20647"/>
        <property type="gene ID" value="BRADI_1g55796v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HCM5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK20647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="103" mass="11177" checksum="AA63109EFAC83D51" modified="2016-01-20" version="1">MGSFFSSPVAVEEQVPKAHEWGLQGCTTEELIDILRREFKCPPDAVMMRPPCPCAGFCNQISRNTSSSSSHFPSEIGPVAIAALRSMSKPPTKLNVVQSVDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HJF3</accession>
    <name>A0A0Q3HJF3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100840492</name>
        <name evidence="2" type="ORF">BRADI_1g69500v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752994.1">
        <property type="nucleotide sequence ID" value="XM_014897508.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752995.1">
        <property type="nucleotide sequence ID" value="XM_014897509.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HJF3"/>
    <dbReference type="STRING" id="15368.A0A0Q3HJF3"/>
    <dbReference type="EnsemblPlants" id="KQK22818">
        <property type="protein sequence ID" value="KQK22818"/>
        <property type="gene ID" value="BRADI_1g69500v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK22821">
        <property type="protein sequence ID" value="KQK22821"/>
        <property type="gene ID" value="BRADI_1g69500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840492"/>
    <dbReference type="Gramene" id="KQK22818">
        <property type="protein sequence ID" value="KQK22818"/>
        <property type="gene ID" value="BRADI_1g69500v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22821">
        <property type="protein sequence ID" value="KQK22821"/>
        <property type="gene ID" value="BRADI_1g69500v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1658288at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HJF3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47689">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47689:SF2">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="196"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="591" mass="67055" checksum="7D25DDFBC97A0D19" modified="2016-01-20" version="1">MSQRRLLLLMRNVRLAAASSSRSFHLPAVASISGSNDGNSSYSFNRRLGSTFGAILIGQAAFFLGLGNNCVLAQDDSAAPASTISEKADANVTSLHRIEDGSVISNEHTVKWRIFTDKARDFFLKGELDEAEKFFKAALHEAKEGFGLGDPHAASALNNLAEFYRLRKEYEKAEPLYVEAIEILEQSFGPDDIRVGAALRNLGQHYHIQRRFDQAQTCYERALKIQGRVMGLGHPDYANTMYLLAKVLSQQRKGKDAEALIRESIRILEEAGLGESPTCIQRMRFLSMELVKLGRLAEAENLQRKILHSLELSKGWDSLDTTIAAETLSFTLQTMGKLKEPEEILERCLSVRKKILSEDHFQVAGILVHLARLTLLQIVSDIKVNNDLSRSNLVKAKQLVNDSIRITEAILNPLRKDQKKLNSRFAMEIERIAATAVLLQALEVVGLIEAARRIQAPAKWVLLLRLPLEHMKHGYFKIDVLRLSDTCRLFIWPDKHLLGVQYTTLRIYYKNKFEKFDYQHVEQALRKCISLYNEPHTRNIVSKAVRQHYVRCLSSLILIIQRNPLDAPQLQDLLGESQQIMKELEEENNMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HYG6</accession>
    <name>A0A0Q3HYG6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein arginine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100833717</name>
        <name evidence="16" type="ORF">BRADI_3g03526v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Methylates arginine residues in proteins such as histone H4.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233796.1">
        <property type="nucleotide sequence ID" value="XM_010235494.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HYG6"/>
    <dbReference type="FunCoup" id="A0A0Q3HYG6">
        <property type="interactions" value="2715"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3HYG6"/>
    <dbReference type="EnsemblPlants" id="KQJ93267">
        <property type="protein sequence ID" value="KQJ93267"/>
        <property type="gene ID" value="BRADI_3g03526v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833717"/>
    <dbReference type="Gramene" id="KQJ93267">
        <property type="protein sequence ID" value="KQJ93267"/>
        <property type="gene ID" value="BRADI_3g03526v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833717"/>
    <dbReference type="OrthoDB" id="1368803at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HYG6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000016">
        <property type="entry name" value="Protein arginine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000003">
        <property type="entry name" value="Protein arginine N-methyltransferase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000029">
        <property type="entry name" value="Protein arginine N-methyltransferase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007857">
        <property type="entry name" value="Arg_MeTrfase_PRMT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035075">
        <property type="entry name" value="PRMT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035248">
        <property type="entry name" value="PRMT5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035247">
        <property type="entry name" value="PRMT5_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738:SF0">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05185">
        <property type="entry name" value="PRMT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17286">
        <property type="entry name" value="PRMT5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17285">
        <property type="entry name" value="PRMT5_TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015894">
        <property type="entry name" value="Skb1_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRMT5 TIM barrel" evidence="14">
        <location>
            <begin position="41"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 arginine-N-methyltransferase" evidence="13">
        <location>
            <begin position="304"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 oligomerisation" evidence="15">
        <location>
            <begin position="476"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="444"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="341"/>
            <end position="342"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="428"/>
            <end position="429"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for specifying symmetric addition of methyl groups" evidence="11">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR015894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQJ93267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="643" mass="72311" checksum="423728AE5F5EB8DC" modified="2016-01-20" version="1">MPLGQRAGDKSDSRYCGVEVLDFPAGDGLPAVLTHSLSSAFDFLLAPLVDPDYRPTPGTVLPVAASDLVLSPSQWSSHIVGKISEWIDLDSEDEQLRLDSELTLKQEIAWASHLSLQACVLPPPRRSSCANYARVVNNILQGLTNMQLWLRIPLEKSEPMDEDRDQSDIVDSWEWWNSFRLLCEHSSQLYVALDILSSLPSMNSLGRWFGEPVRAAILQTDAFLTNARGYPCLSKRHQTLLTGFFNHSVQAIISGRSNHNVSQVAEGVLSGAENHTADTPIRHTLSPYLDYIAYLYQRMDPLPEQERFEINYRDFLQSPLQPLMDNLEAQTYETFEKDTVKYAQYQRAIAKALVDKVSDDEVSTTRTVLMVVGAGRGPLVRASLQAAEETGRKLKVYAVEKNPNAVITLHSLIKLEGWESMVTIISSDMRCWDAPEKADILVSELLGSFGDNELSPECLDGAQRFLKPDGISIPYSYTSFIQPVTASKLHNDIKAHKDIAHFETAYVVKLHRVARLAPPQPVFSFTHPNFPKATNQRYTKLQFKLPQDTGSCLVHGFAGYFDAVLYKDVHLGIEPNTCTPNMFSWFPIFFPLRKPIYIPSESPIEVHFWRCCGATKVWYEWAMMAPSPSPIHNSNGRSYWVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HYU8</accession>
    <name>A0A0Q3HYU8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arabinogalactan protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_3g38550v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HYU8"/>
    <dbReference type="EnsemblPlants" id="KQJ98706">
        <property type="protein sequence ID" value="KQJ98706"/>
        <property type="gene ID" value="BRADI_3g38550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ98706">
        <property type="protein sequence ID" value="KQJ98706"/>
        <property type="gene ID" value="BRADI_3g38550v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HYU8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036297541" description="Arabinogalactan protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ98706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="11872" checksum="0D3C2FBB8DB73C99" modified="2016-01-20" version="1">MARLHLVAVAIAVLFAAATAQAPGASPTPAPRAQPTPPPPPPPAPAPVSPPARPPTMAPPTSAPTPALAPKAAAPAPESVISSPPAPTPDAPTTSPPPPNAASGLAPASAWAFAATVAAVAVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HZS2</accession>
    <name>A0A0Q3HZS2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g06023v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HZS2"/>
    <dbReference type="EnsemblPlants" id="KQJ93659">
        <property type="protein sequence ID" value="KQJ93659"/>
        <property type="gene ID" value="BRADI_3g06023v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66032">
        <property type="protein sequence ID" value="PNT66032"/>
        <property type="gene ID" value="BRADI_3g06023v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66033">
        <property type="protein sequence ID" value="PNT66033"/>
        <property type="gene ID" value="BRADI_3g06023v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93659">
        <property type="protein sequence ID" value="KQJ93659"/>
        <property type="gene ID" value="BRADI_3g06023v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66032">
        <property type="protein sequence ID" value="PNT66032"/>
        <property type="gene ID" value="BRADI_3g06023v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66033">
        <property type="protein sequence ID" value="PNT66033"/>
        <property type="gene ID" value="BRADI_3g06023v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HZS2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ93659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="66" mass="6516" checksum="2C08297675595603" modified="2016-01-20" version="1">MGSCHGPSPDFCWPSHLLQAEVLHVLSGAFSLVAACATITSGTPTTPPSGAEPIKLTTSAGAPGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K3H2</accession>
    <name>A0A0Q3K3H2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g45562v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K3H2"/>
    <dbReference type="EnsemblPlants" id="KQK18932">
        <property type="protein sequence ID" value="KQK18932"/>
        <property type="gene ID" value="BRADI_1g45562v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18932">
        <property type="protein sequence ID" value="KQK18932"/>
        <property type="gene ID" value="BRADI_1g45562v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K3H2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK18932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="124" mass="13316" checksum="E5DCB2BC404C8F89" modified="2016-01-20" version="1">MSLTTDSTCCKYGGSTRFQSCRRMHMCLPPPRPAPAAPLRHAPPPRPSPLLRPASAPRPSPPPHPAPAPRPSSPPHPATAPRPSTPPRPSTPPRMHCRRSLLWPCSLSPTVPYSALLPQSALLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KKC6</accession>
    <name>A0A0Q3KKC6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein IRON-RELATED TRANSCRIPTION FACTOR 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100840404</name>
        <name evidence="11" type="ORF">BRADI_2g61050v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transcription activator that binds to the DNA motif 5'-CACGTGG-3' in the promoter of iron (Fe) deficiency-inducible genes as well as of genes involved in iron homeostasis, thus contributing to basal tolerance to iron deficiency, iron uptake from soil and iron transport, particularly during seed maturation and germination. Promotes the accumulation of mugineic acid family phytosiderophores (MAs). Required for ethylene-mediated signaling during iron deficiency responses. Improves growth and yield, especially in calcareous soil with low iron availability. Promotes iron concentration in shoots and grain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KKC6"/>
    <dbReference type="FunCoup" id="A0A0Q3KKC6">
        <property type="interactions" value="625"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3KKC6"/>
    <dbReference type="EnsemblPlants" id="KQK11593">
        <property type="protein sequence ID" value="KQK11593"/>
        <property type="gene ID" value="BRADI_2g61050v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11593">
        <property type="protein sequence ID" value="KQK11593"/>
        <property type="gene ID" value="BRADI_2g61050v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6106870at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KKC6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18914">
        <property type="entry name" value="bHLH_AtORG2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000074">
        <property type="entry name" value="Transcription factor ORG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF156">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="64"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="35"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK11593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="243" mass="26060" checksum="FACCE24C816A25E5" modified="2016-01-20" version="1">MGHKQLFVDDPFASSISSLEAEAIFSGAGGQWRAGGGLDDRDLSAMPAAANTSSGGSGSPGGGGRKMSHNAYERDRRKQLNELYSSLRSLLPDADHTKKLSIPITVSRVLKYIPELQKEVDGLERKKEELTRANCKPGVIAMKDQNVAPVVSATCLDDKDIMVQVSLLSGMAAAALPMSTCIKILENEGLRLVSSSTSAFGNRTFYNLHLQSGTPVRKRLQHGTIFTRGPPAPNSDEGIILVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KZ14</accession>
    <name>A0A0Q3KZ14_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106865794</name>
        <name evidence="2" type="ORF">BRADI_1g28458v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752093.1">
        <property type="nucleotide sequence ID" value="XM_014896607.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KZ14"/>
    <dbReference type="EnsemblPlants" id="KQK16377">
        <property type="protein sequence ID" value="KQK16377"/>
        <property type="gene ID" value="BRADI_1g28458v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106865794"/>
    <dbReference type="Gramene" id="KQK16377">
        <property type="protein sequence ID" value="KQK16377"/>
        <property type="gene ID" value="BRADI_1g28458v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:106865794"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK16377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="447" mass="48058" checksum="62214F9A568E497B" modified="2016-01-20" version="1">MIVFLDAPLLPFPLCSFPELLPSAPSLPSSPHSCLSGRERRQGVRPNARFFIGSRGRGVCTREMAGEENKAAEDKAGKSQQPIASTAATTTATAGDSDSSVNSSLPGGARPSMASFPRLAAAAASSSMNYNVQQSVTKAIPGHGQTGHEHGDTLGTQQGRNADRDEHWARIRRGHDATVAAGNLKRQRLRGNFGFGHGQPPPHPPQQSNTRGPSSAAPTTLNLLNREPPIDVDAPLMCLACKTMPMRQLPDPRTMHHCVCEDCFKAGSTACPQCRQSSSPAQEKSAVPQFEAALQPHGQSSSSAALLQMLPQAGQASSQAPPVPDVQPTAAVGAAANDPNFDWRTLIVRCWECDNRVAPWTTYCPNVQEHRCVCETCFNNGSTACLFCSAANNPNFDWTTLIVRCWECDNRAAPFTTYYPNVQEHLCVCETCFNNGSTACLICPCRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NJN9</accession>
    <name>A0A0Q3NJN9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AT-hook motif nuclear-localized protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100844734</name>
        <name evidence="4" type="ORF">BRADI_1g35720v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17622.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560568.1">
        <property type="nucleotide sequence ID" value="XM_003560520.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NJN9"/>
    <dbReference type="STRING" id="15368.A0A0Q3NJN9"/>
    <dbReference type="EnsemblPlants" id="KQK17622">
        <property type="protein sequence ID" value="KQK17622"/>
        <property type="gene ID" value="BRADI_1g35720v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844734"/>
    <dbReference type="Gramene" id="KQK17622">
        <property type="protein sequence ID" value="KQK17622"/>
        <property type="gene ID" value="BRADI_1g35720v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844734"/>
    <dbReference type="OrthoDB" id="692562at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3NJN9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014476">
        <property type="entry name" value="AHL15-29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31100:SF20">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31100">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016021">
        <property type="entry name" value="ESCAROLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PPC" evidence="3">
        <location>
            <begin position="98"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR016021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK17622.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="291" mass="29457" checksum="7A9854CCACD24599" modified="2018-03-28" version="1">MASNSNKWWQAPLDFPPQPQPPHHHQHYQQQQQPAVTMPPPSSTAPAAPPAAAASAQSQDQPAPGAAGAMVPLRKPRGRPLGSKNKPKPPVIITRDSPDALHSHVLEVSPGADVSACVAQYARARGRGVCVLGASGTVADVAVRVPGAPAAGALPLTLPGRFELLSVTGTVLPPPAPAEASGLAVLLAAGQGQVLGGRVVGPLVAATPVTLFAATFANAVYERLPLQDDAVVDVKPNVSAADAQQAQQPQELPLAMSQAMAMGGAAGYPVVHRASPPYAWGGGHGHGSGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PFR3</accession>
    <name>A0A0Q3PFR3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Agenet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100824623</name>
        <name evidence="6" type="ORF">BRADI_4g16406v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237643.1">
        <property type="nucleotide sequence ID" value="XM_010239341.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PFR3"/>
    <dbReference type="FunCoup" id="A0A0Q3PFR3">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ88209">
        <property type="protein sequence ID" value="KQJ88209"/>
        <property type="gene ID" value="BRADI_4g16406v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824623"/>
    <dbReference type="Gramene" id="KQJ88209">
        <property type="protein sequence ID" value="KQJ88209"/>
        <property type="gene ID" value="BRADI_4g16406v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824623"/>
    <dbReference type="OrthoDB" id="687110at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3PFR3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014002">
        <property type="entry name" value="Agenet_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007930">
        <property type="entry name" value="DUF724"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31917">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31917:SF74">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05266">
        <property type="entry name" value="DUF724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00743">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Agenet" evidence="5">
        <location>
            <begin position="46"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet" evidence="5">
        <location>
            <begin position="123"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet" evidence="5">
        <location>
            <begin position="196"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="327"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="566"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="804"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="376"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ88209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="875" mass="97604" checksum="E0F240439E81F79C" modified="2016-01-20" version="1">MPGRSPAATGRRRSRSRGWSSSPKGSSRRRPQSPGPSCPQPLAQLPPLPPGTEVEVRVDDDGFHGSWFEATVDSFIPSRGRGHRPRYTVTYSHLLSDDSGATLVEPFAPSRIRPRPPPPATTEPLRLHEIVEAFHNDGWWSGIVLATEPLTVAFPITREVITFQERHHVRPRRDYVDGQWVPSQAAIAVQPKRAVRVYTHNEKVEVVRDRPVYGYSWFPAKVEKVIDGLSYLIKYLEGEEMEYLHCLFIRPAVEHSVLESEFRLGPGAPVEAYCDGAWSPGVVLRHVITDGDYEVCINGKEEKMLPELLKPQYMWNGKHWKIVSAKRQGNRQQSLSGKRPSSPVELASSDDEHIQSWATKRSRKELPQKKPEELTGGSQHALESTMDTTVSALRKSLASSDSPKSCSPKRNNFQVLSQRIVSSCAAPINGLGFCHASSEHPTPQHESRSDVQIQLKGNSDISCKEIICALSASACNIPSPLDKKIRVSNAVSRGAGSGSNPNVFSSKKSAGKQSFIATSTMRKKGTKKVAQRLKESLMELEGRGPHTPQQLSRNLEMHVNEGTNQELLPIEPPGFRSIYNEQGRPHTPQQLSRNLEMNVNEGTNQELLPIEPPGFKSICNEQGFLDGEHVDEEPIHIGSSECTMNDTPLRSYSVVGSSLFSTLPSSHISGQQCSLIKISDVLHLAEKTMEVFRKVPQRPHFRPLEKHAPELREGIAIGLKLGYANLVDSVNNSSIEDSIASFEGKISALTILEENGFEVKSLHHILNKLLEAKLDYSKHVGHRDKLKELVPRQESTVSQKHALLNEKEKTAFQLQQKLDSIRREADQIARERENEDAELLRLKAEVNATQEACSEGELRFRSTLAELRSRLQLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3Q4W2</accession>
    <name>A0A0Q3Q4W2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100821524</name>
        <name evidence="4" type="ORF">BRADI_3g26542v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3Q4W2"/>
    <dbReference type="STRING" id="15368.A0A0Q3Q4W2"/>
    <dbReference type="EnsemblPlants" id="KQJ96673">
        <property type="protein sequence ID" value="KQJ96673"/>
        <property type="gene ID" value="BRADI_3g26542v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96673">
        <property type="protein sequence ID" value="KQJ96673"/>
        <property type="gene ID" value="BRADI_3g26542v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="602113at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3Q4W2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47851:SF5">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47851">
        <property type="entry name" value="OS06G0588700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044546096" description="Myb/SANT-like domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Myb/SANT-like" evidence="3">
        <location>
            <begin position="24"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ96673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="248" mass="28201" checksum="CBF63AC60B1F05B1" modified="2016-01-20" version="1">MQAVDIGYCFLYCLASMIPCMAAEWSDENTRIVTDLFTDQVRAGNRPNTHLTPNAYEEVAKEFKRRTCVDLKHTQLKNKWDKLKIDYNNFKRLKQRETGGGWESGTVTHDGEWWKHAKKDIKGYGKFRKQGLRNEENLRVMFEDITSDGTDHWNPASGLPPPSSAVVANILNVDAIQDVDLDEIEEVSPASSKSGKRLGRFINDKDRKPKTAQVMQEQITKIGNIAERSQASFESFMKDDDAAQSLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QEF6</accession>
    <name>A0A0Q3QEF6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g47955v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3QEF6"/>
    <dbReference type="EnsemblPlants" id="KQK00210">
        <property type="protein sequence ID" value="KQK00210"/>
        <property type="gene ID" value="BRADI_3g47955v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00210">
        <property type="protein sequence ID" value="KQK00210"/>
        <property type="gene ID" value="BRADI_3g47955v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3QEF6"/>
    <dbReference type="OrthoDB" id="671947at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012871">
        <property type="entry name" value="DUF1668_ORYSA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33085:SF85">
        <property type="entry name" value="DUF1618 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33085">
        <property type="entry name" value="OS12G0113100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07893">
        <property type="entry name" value="DUF1668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK00210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="34739" checksum="0AEB555F274B5645" modified="2016-01-20" version="1" fragment="single">RRSPSPIPPVARFDGPHCRISGFVAHGTSILAMPCSTKADSAFPVFDTMTLALSLCAHPNGQKTWSRPVSASVAGKLYMMVGSMVCALGAPPRRCGVLDEGTPRSWTVGAAPLESARIVSYAAHPDGRTLFVSGGESSFCLDTESESLGWTCQGKWVMPFKGQAYYDGELDAWVGLCRYKGGVGYLCSCDVPPAVAAATGGGGMPAWKLGKDKLFSPYNENERHLGAILLHLGNSDYCLIESLVHKDDCLSYVHGNPDWPFERCRVQRRRVFYMTRFGLKYDKNGELRTTRLRARSYGVTEGHVFNEIHSNPVAFWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RJH9</accession>
    <name>A0A0Q3RJH9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">BRADI_1g09000v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RJH9"/>
    <dbReference type="EnsemblPlants" id="KQK13266">
        <property type="protein sequence ID" value="KQK13266"/>
        <property type="gene ID" value="BRADI_1g09000v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13266">
        <property type="protein sequence ID" value="KQK13266"/>
        <property type="gene ID" value="BRADI_1g09000v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3RJH9"/>
    <dbReference type="OrthoDB" id="543442at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RJH9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901001">
        <property type="term" value="P:negative regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000051">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000112">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000517">
        <property type="entry name" value="Putative receptor kinase Lecrk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033238603" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="29"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="296"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="356"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQK13266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="681" mass="75347" checksum="BDA55BC26EBCF918" modified="2016-01-20" version="1">MAMSNMKSTSLLFLKLLYILLNAPSIIAGDVDNHRFVYSGFANASLTLNGTASVTPSGLLELTNGTIMSMGHAFYPAPLRLRDSPNSTVQSFSAFFVFGIISIYDLSSHGLTMLVAPSNDFSKATAVQYLGLFNGSNNGNTTNNIFAIELDTWPNSEFGDINNNHVGIDINSLSSVQSHPAGFFNDQDGTFKNLSLTSQEAMQVWVDYDREKTQVNVTMATLDMATKPKRPTVSARHNLSDVLKDVAYIGFSSSTGKIHTRHYVLGWSFAMNSPAPVINVTMLPKLPRNHPKDSRFWVLEIVLPVATAAVILSLIAIAFLLVRRHLRYAEVRDDWEMEFGPHRFSYKDLFNATGGFENKNLLGVGGFGRVYKGVLPRSRLKVAVKRVSHDSKQGMKEFIAEIVSIGRLQNRNLVQLLGYCRRKGELLLVYEYMPNGSLDKCLYGKKDNDVLTWAQRFRIIRGIASGLLYLHEEWEKVVVHRDIKASNVLLDDEMNGRLGDFGLARLYDHGIDPQTTHVVGTIGYLAPELARSRNATPLTDVFAFGIFVLEVTCGKRPVDQQNTQDSQLVLVDWVIDKAQKGSFGDTVDASLEGRYDVGEASLALKIGLLCSHPFANARPSMRQVMQYLDGEIELPGLSLELLALMQNEGFVVQNEGFDPYIASYPSSTTSIGTMSHVSGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S5E2</accession>
    <name>A0A0Q3S5E2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100829628</name>
        <name evidence="6" type="ORF">BRADI_1g54007v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557384.1">
        <property type="nucleotide sequence ID" value="XM_003557336.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3S5E2"/>
    <dbReference type="STRING" id="15368.A0A0Q3S5E2"/>
    <dbReference type="EnsemblPlants" id="KQK20354">
        <property type="protein sequence ID" value="KQK20354"/>
        <property type="gene ID" value="BRADI_1g54007v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829628"/>
    <dbReference type="Gramene" id="KQK20354">
        <property type="protein sequence ID" value="KQK20354"/>
        <property type="gene ID" value="BRADI_1g54007v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829628"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3S5E2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001209">
        <property type="entry name" value="Os07g0213300 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001333">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN GUN1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="189"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="224"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="259"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="331"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="366"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="401"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="436"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="506"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="541"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="576"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="611"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="751"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK20354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="821" mass="92361" checksum="AFF5E1E98A5C5E0E" modified="2016-01-20" version="1">MSSAPPPSSPAPRAPNPEAPPRVVRPPRRPPRTPGPPPWAERRPSISVDYDRGRRTARVEVDGIGADALPARHRLRVEGSRWQRDWKVSEVTARVLALPRAEAHAVDAVLNCWAGRFARRNFPLLIREITFAGSLQHAVHVFRWMKNQENYCARNDIYGMMIRLHARHSQIDQARGLFFEMQEWRCKPDADIYNSLIHAHARAGQWRWAINIMDDMLRAAIPPSRTTYNNVINACGAAGNWKKALELCKKMTRNGVGPDLVTHNIVLSAFKNGSQYSKAIAYFEMMKGANIAPDTFTLNIVIHCLVKDGQYGEAIELLNSMREKRTQCPPDVVTYTSIMHSYYVCGKVEDCKAVFDMMVAEGVKPNIVSYNALLGAYASRGMHADALGIFKLLKQNGLRPDVVSYTTLLNAYGRSGQPEKAREAFKEMRKNSCRPNIVSYNALIDAYGSAGMFKEAISLLHEMEKDGIPPDVVSISTLLTACGRCRQITKIDTILEAAKSRGIKLNIVCYNSGIGSYLNFGDYGKALELYAVMMASNVNPDAVTYNILISGLCKVGKYAESLKFFEDMVDLRIPLTKEVYSSLICSYVKQGKLTEAESTFSSMKESGCLPDVLTYTAMIEAYNDDGSWRNAWDLFKEMEGNTVQPDAIICSSLMEALNRGSQHERVLQLMELMKEKCIPLNQKAYFEIIASCSMLRDWKTASEIIEHLDSSLSSISVGTLNHLLTFLGKCGKTECMMKLFYKMMSSCSTVGVSTYTVLLRNLLAVGKWRKYIEVLQWMEDAGVSPTLYMYQNVLPYIWRDNSMDYVTLMQEKINSLREKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S6M4</accession>
    <name>A0A0Q3S6M4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small RNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.386</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100832180</name>
        <name evidence="17" type="ORF">BRADI_1g56760v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>small RNA 3'-end nucleotide + S-adenosyl-L-methionine = small RNA 3'-end 2'-O-methylnucleotide + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37887"/>
            <dbReference type="Rhea" id="RHEA-COMP:10415"/>
            <dbReference type="Rhea" id="RHEA-COMP:10416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:74898"/>
            <dbReference type="EC" id="2.1.1.386"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. HEN1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.386" evidence="11"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557559.1">
        <property type="nucleotide sequence ID" value="XM_003557511.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3S6M4"/>
    <dbReference type="FunCoup" id="A0A0Q3S6M4">
        <property type="interactions" value="1525"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3S6M4"/>
    <dbReference type="EnsemblPlants" id="KQK20786">
        <property type="protein sequence ID" value="KQK20786"/>
        <property type="gene ID" value="BRADI_1g56760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832180"/>
    <dbReference type="Gramene" id="KQK20786">
        <property type="protein sequence ID" value="KQK20786"/>
        <property type="gene ID" value="BRADI_1g56760v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832180"/>
    <dbReference type="OrthoDB" id="2154311at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3S6M4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090486">
        <property type="term" value="F:small RNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Hua enhancer1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026610">
        <property type="entry name" value="Hen1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040870">
        <property type="entry name" value="HEN1_dsRBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040813">
        <property type="entry name" value="Hen1_Lam_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21404">
        <property type="entry name" value="HEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21404:SF3">
        <property type="entry name" value="SMALL RNA 2'-O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17842">
        <property type="entry name" value="dsRBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18441">
        <property type="entry name" value="Hen1_Lam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21224">
        <property type="entry name" value="Hen1_LCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Small RNA 2'-O-methyltransferase Hen1 La-motif C-terminal" evidence="16">
        <location>
            <begin position="223"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="HEN1 double-stranded RNA binding" evidence="15">
        <location>
            <begin position="361"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase type 12" evidence="14">
        <location>
            <begin position="718"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="917"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK20786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="944" mass="104804" checksum="828A7DCC6CB310FF" modified="2016-01-20" version="1">MPTATPAPTPKAVIHQRYGAKAVYRVEEVREAVDGGGCPGLALPQQGTRCVYRCELDIAGVLRVATPGNFVRKKDAEQAAAQIALDKLGIQPTANTPATPEEAWEELIGRISYFFADENFPASSHPLVGHLSVSLRRTGDLLGRIPISAIVACDVKVHTLCKIIDPKAEFDPLLVLSMIYNAAKQSPGVSVSDSDFWIQSQRPYSPEAVDLAFQRWSGISDPISVEAVFVPCVMEDEPKTVRLNISQNEHYMGDIASMLSATDSSHVLVSRTVGKTSSEIRFYFPAPNVQLVSDLSNNVASHRGDGNMNRVINKRASYISGQTIYGDAILVNVGYTRRDTELQTERVTLCTYYRILLGKLPDGIYKISKESILVAELPCVYSRTSWKGPSPRDLLCSFCRLQRLSEPHFAANRVRASCNTLGSAVCSEKIGSPKPATGSQYGNDGRIDKENPDVFECDVKIYSKKRELLLEYSTDDDWSKESDAIQNSALKVLVWFNHYFKQLNTLLEKIYLPKSTDGFTIYPNTFSQEFAMCLSVYGKTNGGNSITCSTVGLFPMDPSHQQLENSAFLTDVDGQDSGVFPSHGSLTCIDYIVYLFMKDKRKRYILEVNNEFEFEIGAGAVRNQLESCVTQLSVNQSARFVDELSDRDLFLAAASELSPDLSKISRDSCVLEFSVKVLQVTEPLEDRMEKALFNPPLSKQRVEFAVRHINQLHATTLVDFGCGSGSLLDSLLEHPTTLDKIVGVDISRKGLTRAAKSLHQKLSKKSLMQTAVPTAVLYHGSITDFDSRLYGFDIGTCLEVIEHVEEDQASLFGHVVLSSFCPAVLIVSTPNYEYNPILQRSAMPTKDEEPEENAGPCKFRNHDHKFEWTRSQFQRWATGLAANHNYSVEFSGVGGSGEEPGYASQIAVFRRMARDQVETVPDEDDPPRPYETLWEWPTTSIPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S771</accession>
    <name>A0A0Q3S771_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BRADI_1g57690v3</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3S771"/>
    <dbReference type="FunCoup" id="A0A0Q3S771">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK20946">
        <property type="protein sequence ID" value="KQK20946"/>
        <property type="gene ID" value="BRADI_1g57690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20946">
        <property type="protein sequence ID" value="KQK20946"/>
        <property type="gene ID" value="BRADI_1g57690v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3S771"/>
    <dbReference type="OrthoDB" id="543442at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3S771">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901001">
        <property type="term" value="P:negative regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000051">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000112">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000517">
        <property type="entry name" value="Putative receptor kinase Lecrk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033725069" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="17"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="345"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KQK20946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="667" mass="74608" checksum="CAF24885A23AE75B" modified="2016-01-20" version="1">MMFFLPFLVCFGLSLAFFTATGNDQFLYLGFTGANLTVDDAATIMSNGLLELTNGSLTFKGHAFYPIPLHFRKSYNDTVQSFSVAFIFAIHSSYPILSRHGLAFIVAPSKNFSDALPSQYMGFMNSQNNGNLSNHIFAIELDTVLNLEFQDKDANHIGIDINDLHSVQSHSAGYYDDRSSNFQNMSLVSGDAMQVWVDYNGEAKKINVTMAPLQMEKPTRPLISTYCDLSTVLQEPSYIGFSSSTGEVDSRHYVLGWSFGMNIPAPVINIAKLPKLPRQGPKHQPKLLEIILPIASAALIIAVGAVIILLMRRQLRYAELKEDWEIEFGPHRFSYKDLYQATEGFKSKNLLGAGGFGKVYKGALRSSQKEVAVKKMSHESRQGMKEFVAEVVSIGHIRNRNLVQLLGYCRRKGELLIVYDYMSNGSLDKYLHCGEQKPTLNWAQRFRIIKDIASGLLYLHEKWEKVVIHRDIKASNVLLDSEMNGRLGDFGLARLYDHGTDQQTTHVVGTMGYLAPELISTGKASPLIDVFAFRMFLLEVTCGKRPVSSDAQGNQLVLVDWVLENWRKGSLTEAVDMSLRGDYNVGEACLVLKLGLLCSHPFTNARPDMQQIMQYIDGDLPLPELTHTDMSFSMLYLMQDERFDPHTLSYPSSKASIGTISRISGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3SCJ4</accession>
    <name>A0A0Q3SCJ4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100834046</name>
        <name evidence="9" type="ORF">BRADI_1g68860v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228917.1">
        <property type="nucleotide sequence ID" value="XM_010230615.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3SCJ4"/>
    <dbReference type="EnsemblPlants" id="KQK22698">
        <property type="protein sequence ID" value="KQK22698"/>
        <property type="gene ID" value="BRADI_1g68860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834046"/>
    <dbReference type="Gramene" id="KQK22698">
        <property type="protein sequence ID" value="KQK22698"/>
        <property type="gene ID" value="BRADI_1g68860v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3SCJ4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="516"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="562"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK22698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="65094" checksum="742A470E88493500" modified="2016-01-20" version="1">MDAAEERRLLVQDDGDREPLLPSLQDASHHTGDGSIDIKGHPASKLTTGSWRACFFILGNECCERLAYYGIAKNLVTYLKVKLHQGNFEAARNVTTWQGTCYLTPLIGALLADSYWGKYWTIAVFSSIYFIGLAVLTLSASLPALQPPACLGSVCPEASLLQNGTFFLGLYMIALGTGGIKPCVSSFGADQFDDSDPTERAKQGSFFNWFYFCINIGAFISGTVIVWIQDNSGWGIGFAIPTVFMALAIASFFAASNMYRFQKPGGSPLTRVCQVIVAAFRKWHVEVPHDNCLLYEVDGQNSAIEGSRKLEHTCELEFLDKAAIISSADAKIDFFTNPWRVCTVTQVEELKILVRMFPVWATTIIFNAVYAQNSSLFIEQGMVLDKRVGSFNVPPASLSTFDVISVLIWIPIYDRVLIPIARKFTGREKGFSELQRIGIGLVLSIVAMASAALVELKRLEIARSEGLIHENVAVPMSILWQIPQYFFVGAAEVFTAIGQVEFFYDQAPDAMRSLCAAFALVTVSLGSYLSSMILTLVSYLTTQGGDPGWIPDNLNEGHLDRFFWLIAGISFVNLLVYIGCAMRYRYKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CET3</accession>
    <name>A0A2K2CET3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g01207v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CET3"/>
    <dbReference type="EnsemblPlants" id="PNT60534">
        <property type="protein sequence ID" value="PNT60534"/>
        <property type="gene ID" value="BRADI_5g01207v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60534">
        <property type="protein sequence ID" value="PNT60534"/>
        <property type="gene ID" value="BRADI_5g01207v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CET3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT60534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="84" mass="8967" checksum="2C4762B89B95A1D5" modified="2018-03-28" version="1">MEQESQKTSLPLHTRYSNTTPAAPHRILKSICSESSVCILPKLEIPFTFILWIGDIAASMGCVADNNLELAGGGGCGRTYNAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHW2</accession>
    <name>A0A2K2CHW2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100823130</name>
        <name evidence="9" type="ORF">BRADI_5g17722v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHW2"/>
    <dbReference type="STRING" id="15368.A0A2K2CHW2"/>
    <dbReference type="EnsemblPlants" id="PNT61614">
        <property type="protein sequence ID" value="PNT61614"/>
        <property type="gene ID" value="BRADI_5g17722v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61614">
        <property type="protein sequence ID" value="PNT61614"/>
        <property type="gene ID" value="BRADI_5g17722v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="514967at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SBP-like"/>
    </dbReference>
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        <property type="entry name" value="SBP_dom"/>
    </dbReference>
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        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF222">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="8">
        <location>
            <begin position="111"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="192"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT61614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="388" mass="41815" checksum="2B92BF9364136216" modified="2018-03-28" version="1">MEGDGGAGHRATAPWDLGMQWAPPPATSAYPQHFMPPPPPAAMASAQRQQQELTSLKLGKRPCYLPGWRDGQLAQVGAAGHVDVNGGRRAVAAPEGKRKEKAAAATATAAVARCQVEGCHLALAGAKEYHRRHKVCEAHSKAPRVVVHGAEQRFCQQCSRSHVAGAPFRAVRRPAARFPGWCSLSSVIGQSRRRHALAADPDGARRVPRRPLQQRRPRRAHRREPRRPPRVPLLPRPLRPGCGVGARQLAARARGASAGSSGPRDAGPHAGPHRGRAAQAHHAHAGQGLQQASRGYTRRRSGPRPGRVGAPAGSPCGLTCWGLGSSPVLGYTMHARRRRQVVACFVPEIYFVPWARLEPALFWSLCRPCNISINLSWDSVIYSSCSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CJ68</accession>
    <name>A0A2K2CJ68_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_5g25075v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT62073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CJ68"/>
    <dbReference type="EnsemblPlants" id="PNT62073">
        <property type="protein sequence ID" value="PNT62073"/>
        <property type="gene ID" value="BRADI_5g25075v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62073">
        <property type="protein sequence ID" value="PNT62073"/>
        <property type="gene ID" value="BRADI_5g25075v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CJ68"/>
    <dbReference type="OrthoDB" id="690282at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06222">
        <property type="entry name" value="RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044730">
        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
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        <property type="entry name" value="RNase_H-like_EbsB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
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        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074">
        <property type="entry name" value="BNAC02G40300D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074:SF47">
        <property type="entry name" value="RNASE H TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="71"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="247"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT62073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="47870" checksum="8081907B00716973" modified="2018-03-28" version="1">MGFRDIHCHNLALLAKQAWRLFDQPNSLCATILRAKYFPDGNLLGVVLKKGSSASFGAKLSNLDEQGPEHNEVVWNVIWKLQAPAKVKKILRKTLHGALPCRMVLADRHIKVSGLCPVCGVASKDICHMLFRRPRAMEVWQNLGLLHDVERACLLDMAGSAVLDFLLVCAKQQNRTLDHGLLSETFAIAAWYIWWERRQIVNGKPAQNPKHSATCIRAMIARFSTACNHRASIKGWICPPLGLVKLNVDASFDGDSLSGSMGAVIRDDKGHFIAAADESIPSCPDAHVAEAMAQRLGLELALSYGCHRLVVNANNLEVIDTMKKGAQSATLTAAVYKDCLGLSTEFIRIEYDHCHREANQVAHIVAAISKSAPPRIWDDDPPLDIVRLLVSNVNPITIIKCCLFVKKKKRGSKGTLRVTKLQLYFEAGFIRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMA0</accession>
    <name>A0A2K2CMA0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g12075v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CMA0"/>
    <dbReference type="EnsemblPlants" id="PNT63148">
        <property type="protein sequence ID" value="PNT63148"/>
        <property type="gene ID" value="BRADI_4g12075v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63148">
        <property type="protein sequence ID" value="PNT63148"/>
        <property type="gene ID" value="BRADI_4g12075v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CMA0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT63148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="29" mass="3378" checksum="347E3BC2419C2686" modified="2018-03-28" version="1">MTLQIVHLSALFEIDLSGWRNITRVLCTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMX1</accession>
    <name>A0A2K2CMX1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CDK5RAP1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_4g14840v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CMX1"/>
    <dbReference type="FunCoup" id="A0A2K2CMX1">
        <property type="interactions" value="1631"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CMX1"/>
    <dbReference type="EnsemblPlants" id="PNT63372">
        <property type="protein sequence ID" value="PNT63372"/>
        <property type="gene ID" value="BRADI_4g14840v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63372">
        <property type="protein sequence ID" value="PNT63372"/>
        <property type="gene ID" value="BRADI_4g14840v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CMX1"/>
    <dbReference type="OrthoDB" id="190098at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CMX1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035597">
        <property type="term" value="F:tRNA-2-methylthio-N(6)-dimethylallyladenosine(37) synthase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070900">
        <property type="term" value="P:mitochondrial tRNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006463">
        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
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        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01574">
        <property type="entry name" value="miaB-methiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43020">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43020:SF2">
        <property type="entry name" value="MITOCHONDRIAL TRNA METHYLTHIOTRANSFERASE CDK5RAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00413">
        <property type="entry name" value="CDK5RAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="10">
        <location>
            <begin position="253"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="406"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="9">
        <location>
            <begin position="664"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009815"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT63372"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="760" mass="85074" checksum="75D2DA5D39EA020E" modified="2018-03-28" version="1">MIYFVVLQSSGKIISPLDRSGGSPPAAGDRSRREARSLRLLQVAVGPQPQREPKPQPQPAQAQAPAGDLELPWLFKSIRTVAQGFLIAGDLPSSSDGDGSSWRKRNPGQVAVAADRGDAEQRALAAALVSGRPATVLEFYSTRCRLCASLQGLVRELEDGAGRCVSFVLADAEDDRWLPELLHYDVRAATVLEFYSTRCRLCASLQGLVRELEDGAGGCASFVLADAEDDRWLPELLHYDVRLESGPATAKKGRIYHETYGCQMNVNDMEIVLSIMKKEGYNEIVPDPESAEIIFINTCAIRDNAEQKVWQRLNYFWFLKRQWKSNVAGGRSKSLRPPKIAVLGCMAERLKEKILDSDKMVDVVCGPDAYRDLPRLLQEVDYGQKGINTLLSLEETYADITPVRISDNSVTAFVSIMRGCNNMCSFCIVPFTRGRERSRPVSSIVREVGELWDAGVKEVMLLGQNVNSYNDTSEVEDLEPGKNWQLSEGFSSRCKVKNMGLRFADLLDHLSLEYPEMRFRFTSPHPKDFPDELLYLMRDRHNICKLIHLPAQSGSTEVLERMRRGYTREAYLELVQKIRNVIPDVGLSSDYISGFCGETEDDHADTLSLVRAVGYDMAYMFAYSMREKTHAHRNYEDDVPNDVKQRRLTELINTFRETTRKIYDSQVGTTQLVLVEGPNKRAPETELFGKTDRGHKVSFTSIPVPHTFECDGSRKPVVGDFVEVKILRSSTASLSGEPIARTSLSMYCKNHASDAHAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMZ0</accession>
    <name>A0A2K2CMZ0_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g15154v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
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    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT63401">
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        <property type="gene ID" value="BRADI_4g15154v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_4g15154v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CMZ0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="108" mass="11417" checksum="346587E47AF19E11" modified="2018-03-28" version="1">MMAMLGVVPLVGGIIFGADAGWWDSWMEACGRVATCVATTMVTSGAGSWRGLLRRPFVVFCVCPCSVLCGSGVVGTVALQGFLLCLLVVKSESPARGLLLMTMTPWWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CT34</accession>
    <name>A0A2K2CT34_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g38363v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CT34"/>
    <dbReference type="EnsemblPlants" id="PNT65192">
        <property type="protein sequence ID" value="PNT65192"/>
        <property type="gene ID" value="BRADI_4g38363v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65192">
        <property type="protein sequence ID" value="PNT65192"/>
        <property type="gene ID" value="BRADI_4g38363v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CT34"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT65192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="82" mass="8862" checksum="3C8360F3D84DDA29" modified="2018-03-28" version="1">MPISSNHGELTAPPRPCLQQQEHAGHPPPRLRPDAPASPQRPCGASSASPARARAARRPPSSSPPAVRRRARRHRPRAFPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CV06</accession>
    <name>A0A2K2CV06_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100846821</name>
        <name evidence="11" type="ORF">BRADI_3g03597v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570916.1">
        <property type="nucleotide sequence ID" value="XM_003570868.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CV06"/>
    <dbReference type="FunCoup" id="A0A2K2CV06">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT65859">
        <property type="protein sequence ID" value="PNT65859"/>
        <property type="gene ID" value="BRADI_3g03597v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846821"/>
    <dbReference type="Gramene" id="PNT65859">
        <property type="protein sequence ID" value="PNT65859"/>
        <property type="gene ID" value="BRADI_3g03597v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846821"/>
    <dbReference type="OrthoDB" id="644366at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF906">
        <property type="entry name" value="OS08G0205100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="11"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="174"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="428"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="554"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT65859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="924" mass="103942" checksum="4681E5B6B1845192" modified="2018-03-28" version="1">MAEIVSATSGVMNPLLGKLTALLGEEYKKLTGVRKQASFLKDELSAMKALLDKLELMDEPDPLAKDWRDHVREMSYDMENCIDDFIHDLGVGGADAKVGFVRKTAQRLRRLGRRHKIADRIEELKVLALEVNERRLRYKVDDYINSASGAVPVDPRISAIYKEAAGLVGIDGPREEIVNWLTASVRKLNVVSIVGFGGLGKTTLAKQVYDEIRGKFECMAFVSVSQRPDMTSLLSGLQLKLRVDESRRAHEVTDIIDRLREHLKNKRYLIVVDDLWDQSAWDTIRCVFPEGDNGGTVIVTTRLDDVACAACHDHHGYIYRMKPLAKEDSKRLFFSRVFRSEDGCPPQFQEVSTQILKKCGGLPLAIITIASLLASRQARSRSDWESIKDSLGTNLAAYPTLEGMKNILNLSYINLPLRLRACFLYLGMYPEDREIRRDDLTRQWVAEGFVSGPHGADLEEVAKSYFNELINRSMIQPGKENMYGEVDYCRVHDLMLDLILSKCTENNFLSAAHGYEEMERMHGCNYKIRRLSLSLSAGGAATPGLTAPATSLSQVRSFARFGDSKYTPPLCLFEYLRVLVFEFSGYLDMTIDLSAIGHLFLLRYLKVSAESASINLPVEVKGLVHLETLEMSCLSAQSLPSDVVCLPNLFRLILRCDNGLPQGIRNMKSIRTLHCFGMWKSSVEDIKGLGELTTLRDLQLESRYGGGLTEDGVDALVSSVGKLRGLKRLSLDCQRARYDHRLESLPDHPLPRIEVLDLRWWRLVRVPQWIGGLRCLQVLYLSIAQFSSENVRVPGMLPSLVDATFEVVDIPQDKVVVGTGLFPALERVDFCAREEDATAYLGFEAGAMPKLRTLWFEFGLREWGGVTPGGMHNLLALEQIYVRLWHMGNVTPEQPEQVLRDFESAFDDVSRAHPARPAVSVTWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D047</accession>
    <name>A0A2K2D047_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g30181v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D047"/>
    <dbReference type="EnsemblPlants" id="PNT67656">
        <property type="protein sequence ID" value="PNT67656"/>
        <property type="gene ID" value="BRADI_3g30181v3"/>
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        <property type="component" value="Chromosome 3"/>
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        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="56" mass="6123" checksum="94B7C464EE938939" modified="2018-03-28" version="1">MGIIRGKTGTKRPPPPLLGINISFLRRQVLTNGGTRTTTEGRISTEKHKGDRHIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D095</accession>
    <name>A0A2K2D095_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g30701v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="STRING" id="15368.A0A2K2D095"/>
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    </dbReference>
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        <property type="gene ID" value="BRADI_3g30701v3"/>
    </dbReference>
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        <property type="term" value="P:root development"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="262" mass="28457" checksum="1C16FC7157A533B3" modified="2018-03-28" version="1">MHLTMSSAEKRAGRFRLPRSTLSSRAHLDINVRKLWAWPASGDDDGLTRAESVLATLGGLLAEPRAAAALRAHGDDRILDVFLELADVCGSFGRALLALKQSITELRAVVQRRHGDATATVATALRARRRAEKEIRRLAAAIRRVSRDATVLSDGDSKERCPRRDMTGIVADVAAATAAAPEAIFRRCAAMSPDVSAVAQSVPWLARLRVPPPPATNKVVSEMAAALESLEERIGEIKRGSEKVFRSLLRTRVSLLNIHSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="ORF">BRADI_3g33258v3</name>
    </gene>
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
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        <property type="protein sequence ID" value="PNT67879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A2K2D0S6"/>
    <dbReference type="EnsemblPlants" id="PNT67879">
        <property type="protein sequence ID" value="PNT67879"/>
        <property type="gene ID" value="BRADI_3g33258v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67879">
        <property type="protein sequence ID" value="PNT67879"/>
        <property type="gene ID" value="BRADI_3g33258v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
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        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="102"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT67879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="102" mass="11631" checksum="6F8C5FB1F43D6F18" modified="2018-03-28" version="1" fragment="single">LEKVIEAEKEAARQLVQQKKRDRALIALKKKKSQEELLKQVDTLQMNVEQQMADIELARKQKAVLESLKTGNASFESIQSEINIDDVQTLMDDTAEAKAYQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D587</accession>
    <name>A0A2K2D587_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D587"/>
    <dbReference type="EnsemblPlants" id="PNT69439">
        <property type="protein sequence ID" value="PNT69439"/>
        <property type="gene ID" value="BRADI_3g55405v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69439">
        <property type="protein sequence ID" value="PNT69439"/>
        <property type="gene ID" value="BRADI_3g55405v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D587"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT69439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="70" mass="7631" checksum="D9230BC1308FD680" modified="2018-03-28" version="1">MFELCMGLQGYGTSPGSREARMAAERRQGKLIEAAKRKWETEAAKNLQFKFCALGFALGVGGGYVLRCGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D5Z9</accession>
    <name>A0A2K2D5Z9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g60156v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D5Z9"/>
    <dbReference type="FunCoup" id="A0A2K2D5Z9">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69697">
        <property type="protein sequence ID" value="PNT69697"/>
        <property type="gene ID" value="BRADI_3g60156v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69697">
        <property type="protein sequence ID" value="PNT69697"/>
        <property type="gene ID" value="BRADI_3g60156v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D5Z9"/>
    <dbReference type="OrthoDB" id="686405at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
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            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT69697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="31835" checksum="612D14EE59DC36BC" modified="2018-03-28" version="1" fragment="single">MAVAKVLTRKKFGKGTLMETMYSAWGADREVSFRELEPNLFLLQAFCLGDWKRIMEDGLWLFWNQEIIKQLASRVGEVLAVDPSAVPTIRGDFFRVRMPRFCEVCGLMGHGKLECGSGKHVEEDLQFGEWMLSDEALWRPGTPGMRVGRNFGGQSGRGGGRSNSRGGRPEGCGRGEERVFRRWKPRQPADSGGRKRSSTDAGLNKDDDLEDTVSSPLKPADGHLLRDGGQIMWKQRSRIQWLAAGDKNTKYFHQRARKRKKKNKIQSLSRVDGTTSTKRGEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="1">
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D9K4"/>
    <dbReference type="EnsemblPlants" id="PNT70958">
        <property type="protein sequence ID" value="PNT70958"/>
        <property type="gene ID" value="BRADI_2g20505v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70958">
        <property type="protein sequence ID" value="PNT70958"/>
        <property type="gene ID" value="BRADI_2g20505v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D9K4"/>
    <dbReference type="OrthoDB" id="692729at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT70958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12649" checksum="C217A22A8CA7AED7" modified="2018-03-28" version="1" fragment="single">EPETIAHLMLGCVFSCQVWAIILGRWGKLEWIPSQDSLPADWWSTRLVPLSERRDLSMGIILVWWTIWKHRNAIVFDGARPDVALVTHLIETEGRLWWKAGLFKGPGLVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DE61</accession>
    <name>A0A2K2DE61_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BRADI_2g45800v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DE61"/>
    <dbReference type="EnsemblPlants" id="PNT72537">
        <property type="protein sequence ID" value="PNT72537"/>
        <property type="gene ID" value="BRADI_2g45800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72537">
        <property type="protein sequence ID" value="PNT72537"/>
        <property type="gene ID" value="BRADI_2g45800v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DE61"/>
    <dbReference type="OrthoDB" id="611629at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DE61">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000115">
        <property type="entry name" value="Lysosomal aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000002">
        <property type="entry name" value="Vacuolar aspartic proteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF4">
        <property type="entry name" value="OS01G0663400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036319227" description="Peptidase A1 domain-containing protein" evidence="11">
        <location>
            <begin position="29"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="13">
        <location>
            <begin position="97"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="12">
        <location>
            <begin position="419"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="128"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="PNT72537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="59226" checksum="C3475760C38574D5" modified="2018-03-28" version="1">MGTGPGAVALHHLLLLFLSVVLFHALLAAPAEGLVRVALKKHPVDEHGLAAGEEAQRLLLRRYGHVFNDASAGASSKPSTAAKGGSVTLKNCLNAQYYGEVGIGTPPQNFTVIFDTGSANLWVPSSNCYFSIACYFHPRYNAGQSKTYKKNGFHNNCHWYICLGFLELSSLLITVSLVTKKLLFQHLFSSFAVRKHVEIHYGTGAISGYLSQDSVQVGGVVVKKQDFIEATGEPSITFMFGKFDGILGLGFKEMLYNMVSQGLVGDLIFSFWFNRHAGEGQGGEIVFGGIDPSHHKGNHTYVPVPKKGYWQFDMSDVLIGGNSTGFCKDGCAAMADSGTSLLSGPTAIVTQINKKIGATGVVSQECKAVVSQYGKQILDLLLKQSFVVGLCTFDGAHGVSAGIQSVVDDKVWGSNDIFSKVTCNMCEMAVVWMQHQLAQNQTQEFVLQYINQLCDSFPSPMGESSVDCNRLASMPDIAFSIGGKQFVLTPEQYILKVGEGVATQCISGFTAVDIPPPRGPLWILGDIFMGAYHTVFDYGNLKVGFAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKK4</accession>
    <name>A0A2K2DKK4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">BRADI_1g22200v3</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26 27">Bd21</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">Bd21</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21 29">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 29">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DKK4"/>
    <dbReference type="FunCoup" id="A0A2K2DKK4">
        <property type="interactions" value="2328"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74806">
        <property type="protein sequence ID" value="PNT74806"/>
        <property type="gene ID" value="BRADI_1g22200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74806">
        <property type="protein sequence ID" value="PNT74806"/>
        <property type="gene ID" value="BRADI_1g22200v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DKK4"/>
    <dbReference type="OrthoDB" id="1896041at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DKK4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048508">
        <property type="term" value="P:embryonic meristem development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009942">
        <property type="term" value="P:longitudinal axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009945">
        <property type="term" value="P:radial axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000275">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000192">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000260">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000369">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000679">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF63">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 24" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033311719" description="non-specific serine/threonine protein kinase" evidence="24">
        <location>
            <begin position="42"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="741"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="803"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="831"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="PNT74806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1095" mass="117503" checksum="2434E9F069C4BC89" modified="2018-03-28" version="1">MRRRHPPPPPPLPMATLRSQTLPTLLLLLLLLLLLVTAVRASGGGGDGERESLLRFKAAVTADPGGLLRDWSPASADHCRWPGVSCGASGEVVALNFSSSSTGRLSGALSPSVAALRGLRVLALPSHVFSGPLPAAIWSLRRLLVLDLSGNRLHGEIPPSLACAALQTLDLAYNRINGSLPAALGSLLGLRRLSLASNRLGGAIPDELGGAGCRSLQFLDLSGNLLVGGIPRGLGNCSKLETLLLSSNLLDDVIPPEIGWLRNLRALDVSRNSLSGPLPAELGACVELSVLVLSNPYALVGDSDVSNNGDVEDFNYFQGGIPDVVAALPKLRVLWAPRATLEGELPSNWSSCQSLEMMNLGENLFSGGIPKGLLDCGHLKFLNLSSNKFTGSVDPSLPVPCMDVFDVSGNRLSGLIPEFISKGCPSSQLPFDDLVSEYSSLFSYQEIAGFFSFSLVTGTDMTSCHSFARNNFTGTVTSLPLAAEKLGMQGGYAFLADGNNLAGELQHSLFNKCNSSRGFIVDFSDNLITGGIPVEIGSLCRSLVVLRVAGNRLSGLIPTSIVQLNYLISLDLSRNQLGGEIPSIVKNLPHLELLSLGHNLLNGTIPSDINQLRSLKVLDLSSNFLTGEIPRTLADLTNLTALLLDNNKLTGKIPAEFANSASLTVFNVSFNNLSGTVPTNNSTVGCDSVIGNPLLQSCRMYSLAVPSAAQQSRGLNSNDSDTAPADSQNQLGNSSFNAIEIASITSATAIVSVLLALIVLFVYTRKCAPRMAGRSSGRREVIIFQEIGVPITYETVVRATGNFNASNCIGSGGFGATYKAEISPGVLVAIKRLSVGRFQGAQQFHAEIKTLGRLRHPNLVTLVGYHLGESEMFLIYNYLPGGNLERFIQERSKRPVDWKRLHKIALDIAKALAYLHDTCVPRILHRDVKPNNILLDTNHNAYLSDFGLARLLGNSETHATTGVAGTFGYVAPEYAMTCRVSDKADVYSYGVVLMELISDKKALDPSFSPYGNGFNIVAWACMLLRQGRAREFFVDGLWDVGPHDDLVEVLHLAVMCTVESLSVRPTMKLVVQRLKQLQPPIREHRKTWDLSEELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKL6</accession>
    <name>A0A2K2DKL6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g22551v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DKL6"/>
    <dbReference type="EnsemblPlants" id="PNT74816">
        <property type="protein sequence ID" value="PNT74816"/>
        <property type="gene ID" value="BRADI_1g22551v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74816">
        <property type="protein sequence ID" value="PNT74816"/>
        <property type="gene ID" value="BRADI_1g22551v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DKL6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT74816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="102" mass="11299" checksum="1C2D917079F9BAF7" modified="2018-03-28" version="1">MYRHAPSNSLSPSRTKRSQCLERRRRQTLAPPPSRARGFHGGGRDARTLAAAPSHTQARVVAVDAAALGVLHREGWSRSLGRPSQRRSRSTRRGRPGRRPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DLX8</accession>
    <name>A0A2K2DLX8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g29443v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DLX8"/>
    <dbReference type="EnsemblPlants" id="PNT75279">
        <property type="protein sequence ID" value="PNT75279"/>
        <property type="gene ID" value="BRADI_1g29443v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75279">
        <property type="protein sequence ID" value="PNT75279"/>
        <property type="gene ID" value="BRADI_1g29443v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DLX8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT75279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="93" mass="10091" checksum="A35F7F6D0F01D56F" modified="2018-03-28" version="1">MAGAADRLEKRAAAGRDSLRDLRHVVFCSGESSDGGANPWRWLLFLCFLQDLLMSLKTAGLADQFHRSADARAFGIGTFWSAVLAEKPSGFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DM92</accession>
    <name>A0A2K2DM92_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g31656v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DM92"/>
    <dbReference type="EnsemblPlants" id="PNT75399">
        <property type="protein sequence ID" value="PNT75399"/>
        <property type="gene ID" value="BRADI_1g31656v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75399">
        <property type="protein sequence ID" value="PNT75399"/>
        <property type="gene ID" value="BRADI_1g31656v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DM92"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="10402" checksum="AB502D9524EED476" modified="2018-03-28" version="1">MSASLLSDLVCAHRGQSSAPPTSSPPAAWRQGQQQELRHHEDRDEVKGAGRWRRREMQPRPYHLLYLKEMHTLVYAMTMDQKESMPQRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DSI1</accession>
    <name>A0A2K2DSI1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g59792v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DSI1"/>
    <dbReference type="EnsemblPlants" id="PNT77236">
        <property type="protein sequence ID" value="PNT77236"/>
        <property type="gene ID" value="BRADI_1g59792v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77236">
        <property type="protein sequence ID" value="PNT77236"/>
        <property type="gene ID" value="BRADI_1g59792v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DSI1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT77236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="146" mass="16091" checksum="8BA00C85AEBF5A32" modified="2018-03-28" version="1">MKTWVSDRLARFVPSFHSITSTAPRSHAAAILDLRPLSSCLSTTGLASAPICFSDVPPGHLPHALDRRHAFVGTDRRRPRSSTPAPPFLPPPPPRWSFAIPSGEHFHLPSPAPPRPPNPLGHTTPAQTPQIPLFHFHHARCRRLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DT35</accession>
    <name>A0A2K2DT35_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g62785v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DT35"/>
    <dbReference type="EnsemblPlants" id="PNT77436">
        <property type="protein sequence ID" value="PNT77436"/>
        <property type="gene ID" value="BRADI_1g62785v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77436">
        <property type="protein sequence ID" value="PNT77436"/>
        <property type="gene ID" value="BRADI_1g62785v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DT35"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT77436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="209" mass="20456" checksum="C2832A6E96FFE2CA" modified="2018-03-28" version="1">MDGFRTRAPLFHTAKAGRDEEAAIEARVLLVVATGRNQAVAWTSLGGIMQRRLGAELARGLQQRVAAGLGRRLQQPGVTHAGATAELAAAGSAPEGSGSWGSGGGPRAAAGAAHGRGRRAAGLAGEVGATSGRGGGARGGGRGSARRGLRGRSGPRAGAAAGLAGVAGARGGPVAGEAPGGGARVVCDGDAPAECCESERKRKRMRKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DT80</accession>
    <name>A0A2K2DT80_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100841806</name>
        <name evidence="11" type="ORF">BRADI_1g63575v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230159.3">
        <property type="nucleotide sequence ID" value="XM_010231857.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DT80"/>
    <dbReference type="STRING" id="15368.A0A2K2DT80"/>
    <dbReference type="EnsemblPlants" id="PNT77485">
        <property type="protein sequence ID" value="PNT77485"/>
        <property type="gene ID" value="BRADI_1g63575v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841806"/>
    <dbReference type="Gramene" id="PNT77485">
        <property type="protein sequence ID" value="PNT77485"/>
        <property type="gene ID" value="BRADI_1g63575v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841806"/>
    <dbReference type="OrthoDB" id="759142at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="56"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT77485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="250" mass="27054" checksum="B5D4A2DB50E8AB3F" modified="2018-03-28" version="1">MCSNESRLSRTGASTAARSRSHASQMSTLTRLIAAILLSAAVVVEPLVFDVPSGSSKCLTEFLGRGGHSHVSYRVAEASSRSAVSMRVTGPDGEELHLTEGVDGAGEFMFEAAEGGSYIACFWTPHYERRAMVSVDVQWNANIRAPHTEGPLITFLRARAEGPLPAVNLATAKKGSTDYIAELKNLEDSARLIHEEMISLGGREGEMQRLNESTAVRICSFTLMSLAVCAGVAALQLWHLKAFFQKLRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLX7</accession>
    <name>I1GLX7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100842629</name>
        <name evidence="3" type="ORF">BRADI_1g04790v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564162.1">
        <property type="nucleotide sequence ID" value="XM_003564114.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLX7"/>
    <dbReference type="EnsemblPlants" id="KQK12596">
        <property type="protein sequence ID" value="KQK12596"/>
        <property type="gene ID" value="BRADI_1g04790v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842629"/>
    <dbReference type="Gramene" id="KQK12596">
        <property type="protein sequence ID" value="KQK12596"/>
        <property type="gene ID" value="BRADI_1g04790v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842629"/>
    <dbReference type="eggNOG" id="KOG2640">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051582_1_0_1"/>
    <dbReference type="OMA" id="VCNYEFE"/>
    <dbReference type="OrthoDB" id="1899781at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02999">
        <property type="entry name" value="PDI_a_ERp44_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044794">
        <property type="entry name" value="APRL5/7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47126:SF3">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47126">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE-LIKE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093933" description="Thioredoxin domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="55"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK12596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="305" mass="33173" checksum="0DF958B092AE47B5" modified="2012-06-13" version="1">MRRAAVAVTVAAVVSFLLVAAGAGAAGSPGAGGLGSTCPRLGPPFLDALGSRCPRARIEPSPPLEVRGEALDKQLNLRRSGASYSILFYAAWCPFSSKFRPIFEALSTMFPQIHHFAVEESSAMPSLFSRYGVRGFPAVLLVNETSMVRYRGTKDLKSLIDFYKETTGLNPVAYFDLDQQESTRSLGSVMPGGWTLRKMANDEPFVLLSVLFLIMKVVARFVPTVISQMRVFLIVRVRNLNLGISRGSSQLMERALTVLDVKRIWSKLRLSSKTSDLTKGASNARAWASSFASVSLGEPSSSRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNV7</accession>
    <name>I1GNV7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adenylyl cyclase-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100833638</name>
        <name evidence="5" type="ORF">BRADI_1g10340v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CAP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560473.1">
        <property type="nucleotide sequence ID" value="XM_003560425.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNV7"/>
    <dbReference type="FunCoup" id="I1GNV7">
        <property type="interactions" value="2023"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GNV7"/>
    <dbReference type="EnsemblPlants" id="KQK13468">
        <property type="protein sequence ID" value="KQK13468"/>
        <property type="gene ID" value="BRADI_1g10340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833638"/>
    <dbReference type="Gramene" id="KQK13468">
        <property type="protein sequence ID" value="KQK13468"/>
        <property type="gene ID" value="BRADI_1g10340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833638"/>
    <dbReference type="eggNOG" id="KOG2675">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015780_1_0_1"/>
    <dbReference type="OMA" id="KSQQTHK"/>
    <dbReference type="OrthoDB" id="1601at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GNV7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008179">
        <property type="term" value="F:adenylate cyclase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019933">
        <property type="term" value="P:cAMP-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.330:FF:000001">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.70:FF:000006">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.330">
        <property type="entry name" value="Adenylate cyclase-associated CAP, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001837">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013912">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013992">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018106">
        <property type="entry name" value="CAP_CS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053950">
        <property type="entry name" value="CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036222">
        <property type="entry name" value="CAP_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652:SF0">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08603">
        <property type="entry name" value="CAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21938">
        <property type="entry name" value="CAP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01213">
        <property type="entry name" value="CAP_N-CM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101278">
        <property type="entry name" value="N-terminal domain of adenylylcyclase associated protein, CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01088">
        <property type="entry name" value="CAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="4">
        <location>
            <begin position="327"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="222"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="222"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK13468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="487" mass="51966" checksum="CB828C4D11C73C83" modified="2012-06-13" version="1">MEKALVERLEAAVARLEAAAASGASFSSAPRDFGEVPGASDPAIVAYDEFVAQAIGRLTAAAEKIGGKVLDATKVLAEAFTVAKDLLIQAKQLQKPASMANAQDFFKPLNDVIGKAIAMTEGRRPDYFNHMKSVADSLAALAWVAFLGKDCGMSFPTAHVEESWQMAEFYNNKVLVEYRNKDPDHVEWAKALKELYVPGLRDYVKKYYPLGPVWGPVGGAPVSQPKAAAPTPKAPAVKAPPPPAPPSAPLFSTEKSPKSAKPKEGMSAVFQEISSGKPVTSGLRKVTDDMKTKNRADRSGVVNSTAATAPAPEKTSRGASFAFKSGPPKLELQMGRKWVVENQVGKKDLAIDDCDSRQSIYVYGCKDSVLQVNGKVNNITVDKCAKFGIVFKDVVAAFEVVNCNGVEVQCQGTAPTISIDNTSGCQLYLSKHSLGASITSAKSSEMNVLVPSGVTDGDWVEHALPQQYIHSFKDGQFITSPVSHSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQ25</accession>
    <name>I1GQ25_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100834680</name>
        <name evidence="16" type="ORF">BRADI_1g13910v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class III subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562005.1">
        <property type="nucleotide sequence ID" value="XM_003561957.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQ25"/>
    <dbReference type="STRING" id="15368.I1GQ25"/>
    <dbReference type="EnsemblPlants" id="KQK14011">
        <property type="protein sequence ID" value="KQK14011"/>
        <property type="gene ID" value="BRADI_1g13910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834680"/>
    <dbReference type="Gramene" id="KQK14011">
        <property type="protein sequence ID" value="KQK14011"/>
        <property type="gene ID" value="BRADI_1g13910v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834680"/>
    <dbReference type="eggNOG" id="ENOG502QPKR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012517_0_0_1"/>
    <dbReference type="OMA" id="ADPNGCN"/>
    <dbReference type="OrthoDB" id="125004at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08875">
        <property type="entry name" value="START_ArGLABRA2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000197">
        <property type="entry name" value="Homeobox-leucine zipper protein REVOLUTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044830">
        <property type="entry name" value="HD-Zip_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013978">
        <property type="entry name" value="MEKHLA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950:SF7">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08670">
        <property type="entry name" value="MEKHLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="30"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="15">
        <location>
            <begin position="174"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="32"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="141"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="100"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK14011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="861" mass="93546" checksum="144386EC156613D6" modified="2012-06-13" version="1">MAGAMVAGLHGGGRDRDRDRSSPGGGGAPQVDTGKYVRYTPEQVDALERVYSECPKPSSLRRQQLIRECPILSNIEPKQIKVWFQNRRCREKQRKEASRLQTVNRKLSAMNKLLMEENDRLQKQVSRLVYENGYMRTHLSNPSVATTDTSCESVVTSGQHQQQQNPAVPRPQRDANNPAGLLAIAEETLAEFLSKATGTAVDWVQMVGMKPGPDSIGIIAVSHNCSGVAARACGLVSLEPTKVAEILKDRPSWYRDCRCVDVLQIIPTGNGGTIELIYMQTYAPTTLAAPRDFWILRYTSGLEDGSLVICERSLTQATGGPSGPNTPNFVRAEVLPSGYLIRPCEGGGSMIHIVDHVDLDAWSVPEVLRPLYESPKILAQKMTIAALRHIRQIAHESSGEMPYGGGRQPAVLRTFSQRLSRGFNDAVNGFLDDGWSLMSSDGAEDVTIAINSSPNKLVGSHVNSSQLFSAIGGGILCAKASMLLQNVPPALLVRFLREHRSEWADPGVDAYSAAALRASPYAVPGLRASGFMGSQVILPLAHTLEHEEFLEVIRLEGHSLCHDEVVLSRDMYLLQLCSGVDENAAGACAQLVFAPIDESFADDAPLLPSGFRVIPLDAKTDAPSATRTLDLASTLEVGSGGTRAASDAPSTSNTRSVLTIAFQFSYENHLRESVASMARQYVRTVVASVQRVAMAIAPSRLGGQIEMKNPPGSPEAHTLARWIGKSYRFHTGAELLCTDSQSADASLKALWQHSDSIMCCSLKAAPVFTFANQAGLDMLETTLIALQDISLEKILDDDGRKALCSEFPKIMQQGFAYLPGGVCVSSMGRPVSYEQAVAWKVLSEDDTPHCLAFMFVNWSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSQ7</accession>
    <name>I1GSQ7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g22580v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 79 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSQ7"/>
    <dbReference type="STRING" id="15368.I1GSQ7"/>
    <dbReference type="EnsemblPlants" id="KQK15409">
        <property type="protein sequence ID" value="KQK15409"/>
        <property type="gene ID" value="BRADI_1g22580v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15409">
        <property type="protein sequence ID" value="KQK15409"/>
        <property type="gene ID" value="BRADI_1g22580v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1671743_0_0_1"/>
    <dbReference type="InParanoid" id="I1GSQ7"/>
    <dbReference type="OrthoDB" id="726732at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005199">
        <property type="entry name" value="Glyco_hydro_79"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363">
        <property type="entry name" value="HEPARANASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363:SF13">
        <property type="entry name" value="OS07G0598400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03662">
        <property type="entry name" value="Glyco_hydro_79n"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094083" evidence="2">
        <location>
            <begin position="25"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK15409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="158" mass="16223" checksum="F2EB9B5C848665E7" modified="2012-06-13" version="1">MAAPAGGGLILLCTLIFLFSGVRAAGSGVEKATVKVQAVTTIARTDANFICATLDCTTVTFGLNALRGRRKASSNGTLYVGAWDGRNARDLMRYTVEKGYRVESWELGNELCAGGVAAKVDGVRGVGGGGHGLAPGAAQAGAVWRRRKGEKGGVPPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTF2</accession>
    <name>I1GTF2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="4">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC100830084</name>
        <name evidence="21" type="ORF">BRADI_1g24620v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Alternative NADH-ubiquinone oxidoreductase which catalyzes the oxidation of mitochondrial NADH does not translocate protons across the inner mitochondrial membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562875.1">
        <property type="nucleotide sequence ID" value="XM_003562827.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312465.1">
        <property type="nucleotide sequence ID" value="XM_024456697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GTF2"/>
    <dbReference type="FunCoup" id="I1GTF2">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GTF2"/>
    <dbReference type="EnsemblPlants" id="KQK15734">
        <property type="protein sequence ID" value="KQK15734"/>
        <property type="gene ID" value="BRADI_1g24620v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74927">
        <property type="protein sequence ID" value="PNT74927"/>
        <property type="gene ID" value="BRADI_1g24620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830084"/>
    <dbReference type="Gramene" id="KQK15734">
        <property type="protein sequence ID" value="KQK15734"/>
        <property type="gene ID" value="BRADI_1g24620v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74927">
        <property type="protein sequence ID" value="PNT74927"/>
        <property type="gene ID" value="BRADI_1g24620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830084"/>
    <dbReference type="eggNOG" id="KOG2495">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021377_1_3_1"/>
    <dbReference type="OMA" id="DLVWGDW"/>
    <dbReference type="OrthoDB" id="3244603at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000009">
        <property type="entry name" value="Internal alternative NAD(P)H-ubiquinone oxidoreductase A1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF4">
        <property type="entry name" value="NADH:UBIQUINONE REDUCTASE (NON-ELECTROGENIC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="19">
        <location>
            <begin position="117"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="20">
        <location>
            <begin position="487"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="69"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQK15734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="558" mass="60295" checksum="E943DF22CC83BF69" modified="2012-06-13" version="1">MAWSRMARSSQLSRSISRMASGSGAPTPASSALRNAEAANAHRGAAHSFHSLALVGLSDKCGAGHLLHPNRGISTTPPTLHPAGAAAAAEPVECSDAEDHSAAVPDLGPTKPGEKPRVVVLGTGWAAFRFLKDVDTSAYDVVCVSPRNHMVFTPLLASTCVGTLEFRSVVEPVSRIQSALATRPGSFFFLANCTGVDTRRHEVHCTVASDEAAVGTLPRNPYRFRVAYDKLVIASGAEPLTFNIKGVEENAVFLREVSHAQEIRRKLLTNLMLSENPGLSEEEKKRLLHCVVVGGGPTGVEFSGELSDFITRDVRERYAHVKDYVKVTLIEANEILSSFDIGLRQYATNHLSKYGVKLVRGVVKEVEPTKIVLSDGTSVPYGLLVWSTGVGPSEFVKSLDLPKSPGGRIGVDEWLRVPSADDVYALGDCAGFLERTGKPVLPALAQVAEREGKYLAALLKRVAAQNGGKAHCCGKKTDLGEPFVYKHLGSMASVGRYKALVDLRENKDAKGVSMAGFVSWLVWRSAYLTRVVSWRNRFYVAVNWATTLVFGRDNTRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1GW83</accession>
    <name>I1GW83_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100842933</name>
        <name evidence="13" type="ORF">BRADI_1g32840v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the early nodulin-like (ENODL) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563420.1">
        <property type="nucleotide sequence ID" value="XM_003563372.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GW83"/>
    <dbReference type="STRING" id="15368.I1GW83"/>
    <dbReference type="EnsemblPlants" id="KQK17171">
        <property type="protein sequence ID" value="KQK17171"/>
        <property type="gene ID" value="BRADI_1g32840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842933"/>
    <dbReference type="Gramene" id="KQK17171">
        <property type="protein sequence ID" value="KQK17171"/>
        <property type="gene ID" value="BRADI_1g32840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842933"/>
    <dbReference type="eggNOG" id="ENOG502RZQI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058719_1_0_1"/>
    <dbReference type="OMA" id="INPQENY"/>
    <dbReference type="OrthoDB" id="691587at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11019">
        <property type="entry name" value="OsENODL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000010">
        <property type="entry name" value="Early nodulin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041846">
        <property type="entry name" value="ENL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF253">
        <property type="entry name" value="EARLY NODULIN-LIKE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094108" description="Phytocyanin domain-containing protein" evidence="11">
        <location>
            <begin position="24"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="12">
        <location>
            <begin position="24"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="131"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK17171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="272" mass="26120" checksum="C20091642F45940F" modified="2012-06-13" version="1">MASSRGGVARLLCLAMAAAAASAAQFRVGEQRGWSVPDGGAEPYNSWAGRMRFVIGDQLLFVYPKGSDSVLVVDAGAYGSCNTTAYTAKFEDGNTVVTLDRSGPFYFISGNEAGCKANQKLEVVVLAAAHTPPPAPVSPSPSSMPPSPASPPSSMTPPMASPPSPGPSSMTPPMASPPSPGPSSAPPMPAPAAGPGASAPAPSPTGAAAPADSPPAPGSPSSSSSPDGPGGPASPPGGSQGAGSTPGSGAASVTAGILGALAAGVGYAMLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GW97</accession>
    <name>I1GW97_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3615 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100845879</name>
        <name evidence="4" type="ORF">BRADI_1g32960v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563429.1">
        <property type="nucleotide sequence ID" value="XM_003563381.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GW97"/>
    <dbReference type="FunCoup" id="I1GW97">
        <property type="interactions" value="493"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK17194">
        <property type="protein sequence ID" value="KQK17194"/>
        <property type="gene ID" value="BRADI_1g32960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845879"/>
    <dbReference type="Gramene" id="KQK17194">
        <property type="protein sequence ID" value="KQK17194"/>
        <property type="gene ID" value="BRADI_1g32960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845879"/>
    <dbReference type="eggNOG" id="ENOG502R3H2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035562_3_1_1"/>
    <dbReference type="OMA" id="IMECAYD"/>
    <dbReference type="OrthoDB" id="684795at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GW97">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33326:SF14">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33326">
        <property type="entry name" value="OS05G0543800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12274">
        <property type="entry name" value="DUF3615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3615" evidence="3">
        <location>
            <begin position="374"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="323"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="227"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK17194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="491" mass="56002" checksum="BCE2F77AD186AB06" modified="2012-06-13" version="1">MAGAGDPHRPPPPRGTVGWSYRDMEEQTESHEEYISRLLGERTSEPQGDPSGEPSCEPNPLWLHNGDMQIRDERQALEAARRAQNQKRAKAERKAKKKRRRAALALQLEQGTSQPQQHPSGESQPPQDPSLHRPQLIQSPEPARARVRQSVLYADRPRRSRRPSQDKLQVEEHAGMVSSREFEALTLGQSPPTHDSAHRIMECAYDTANSDTAPEIIMTGSESIEVVDKLKDESDDLEELRRSAKEYRAEYDSLSRKEWRAKLSAAHAANLAAILERDAARRRLPDALKWAKWESPPVPMQKPWTSPGSQLLCMLGLSSENDESSVVAHPRDEHLSRQHPPGSQPGSNDSDEDYEELSDQQIIENGRKWMSEEVMVAFKKYTESCADQTGLDYQFGELLHQCLNVEDYYKTYHHFNFTMKMKDPTSCVWRVELYFAEIRRMFRMKHYFCCPLEPLEDGHCYGCKNQGVNDLKHPATGGFDQGCATSTFPYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXJ1</accession>
    <name>I1GXJ1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase III subunit RPC6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100840897</name>
        <name evidence="7" type="ORF">BRADI_1g36620v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563664.1">
        <property type="nucleotide sequence ID" value="XM_003563616.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXJ1"/>
    <dbReference type="STRING" id="15368.I1GXJ1"/>
    <dbReference type="EnsemblPlants" id="KQK17767">
        <property type="protein sequence ID" value="KQK17767"/>
        <property type="gene ID" value="BRADI_1g36620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840897"/>
    <dbReference type="Gramene" id="KQK17767">
        <property type="protein sequence ID" value="KQK17767"/>
        <property type="gene ID" value="BRADI_1g36620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840897"/>
    <dbReference type="eggNOG" id="KOG3233">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033661_2_0_1"/>
    <dbReference type="OMA" id="VGTTKKC"/>
    <dbReference type="OrthoDB" id="613763at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000116">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007832">
        <property type="entry name" value="RNA_pol_Rpc34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016049">
        <property type="entry name" value="RNA_pol_Rpc34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12780">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05158">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK17767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28450" checksum="122821AAA7068065" modified="2012-06-13" version="1">MPPRKRPLEPSSGKPPPPDVKAIVPPPSDPPAAAQEIPPISAAILNNLPGPEREVYKRVYAAGNKGIWSQDLRHSMQLAAPTVTKLTRALVQRGILKEVTDVRHRGKKVFMDSRMEPAPEITGGTWYHNGQLDTEAVAAVRRRCLDQIDRLGAATPDMVHKGVEREDPRAGYMIDQIRDILKTMALDRVLEECRSTGEGEFSAVRSGRVCYRRGGAPQGGMIEGIPCGVCPRIDECSPDGVISPTTCVYYKKWLQMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXW5</accession>
    <accession>A0A0Q3H511</accession>
    <name>I1GXW5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100829968</name>
        <name evidence="4" type="ORF">BRADI_1g37660v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17935.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014755610.1">
        <property type="nucleotide sequence ID" value="XM_014900124.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXW5"/>
    <dbReference type="STRING" id="15368.I1GXW5"/>
    <dbReference type="EnsemblPlants" id="KQK17935">
        <property type="protein sequence ID" value="KQK17935"/>
        <property type="gene ID" value="BRADI_1g37660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829968"/>
    <dbReference type="Gramene" id="KQK17935">
        <property type="protein sequence ID" value="KQK17935"/>
        <property type="gene ID" value="BRADI_1g37660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829968"/>
    <dbReference type="eggNOG" id="ENOG502RYM2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063778_1_1_1"/>
    <dbReference type="OMA" id="EQKPHEI"/>
    <dbReference type="OrthoDB" id="1931904at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GXW5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025520">
        <property type="entry name" value="DUF4408"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33098">
        <property type="entry name" value="COTTON FIBER (DUF761)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33098:SF119">
        <property type="entry name" value="OS06G0566500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14364">
        <property type="entry name" value="DUF4408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4408" evidence="3">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK17935.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="244" mass="26072" checksum="FC0CDC31483C1024" modified="2012-06-13" version="1">MLESAIPALLSTVHGWFTPAVLFVVLNIVIGTIAVTSKVAAASGSRDGAAAGSGAGAGAGGEKRGLSRVPSMALDRLRSFNLSSFAAPAPEPAVTGVLDLGLDEQIPALETDDEGEPEHAPMERSMSEAAAEAELPRLPARLRKSASEKSAFAHFVAEEDTETVEARRPATTRDAGRRRTLAAEPEEPAWEEEIEEAAAEVDARADDFINKFRHQLKLQRIDSFIRYRDTLRRGQQAATAGAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXY4</accession>
    <name>I1GXY4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100833334</name>
        <name evidence="10" type="ORF">BRADI_1g37800v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/BELL homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010227597.1">
        <property type="nucleotide sequence ID" value="XM_010229295.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXY4"/>
    <dbReference type="STRING" id="15368.I1GXY4"/>
    <dbReference type="EnsemblPlants" id="KQK17968">
        <property type="protein sequence ID" value="KQK17968"/>
        <property type="gene ID" value="BRADI_1g37800v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833334"/>
    <dbReference type="Gramene" id="KQK17968">
        <property type="protein sequence ID" value="KQK17968"/>
        <property type="gene ID" value="BRADI_1g37800v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833334"/>
    <dbReference type="eggNOG" id="KOG0773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031764_0_0_1"/>
    <dbReference type="OMA" id="YMANDAM"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006563">
        <property type="entry name" value="POX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07526">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="418"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="420"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK17968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="509" mass="55405" checksum="20E18701E6DD3DAA" modified="2012-06-13" version="1">MSSSDPSYQQLGLDAISTCFIPSSSGSMMMCSSEAPFFHPSIVVPHDASFLSGSGSGVGADVAAHYNYMANDAMETDEDAYAGAESCSTVHSMSMLPAFAAGPLGFFQYGGPADVTIAQPPSRMSKLITGEPHCNSWLYDGPSAVSIHEPYYLAPFSGAGSFPAASGLSLRLGAALSSSVTMASLPEQSSDVSCSGLTHANSEGFGYQQQPETTVKAHAESDDGMPMPFQLPPYPEMYSTPPQLSQVLPRSRYAHVAQELLNGFAACLLNDVADHNISDFGPGNGGIGSEASSNKLMLPSIEQRQDDVRGDLLRLLQLMDQRCNRCFDDIQATASRFSSMVAHSGGGGGGGAIVAAPRFAHRAVWATYQRLRKRITGMMVAVAQREQQPSSLADKERRWESSFIQKHWALQQLRRGDQQSWRPQRGLPEKSVAVLKAWMFENFLRPYPKDNEKDMLAARSGLSRSQVSNWFINARVRLWKPMIEEMYEELKRSSGGREVEHLSSKDVVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZS4</accession>
    <name>I1GZS4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100846584</name>
        <name evidence="3" type="ORF">BRADI_1g45790v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564090.1">
        <property type="nucleotide sequence ID" value="XM_003564042.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZS4"/>
    <dbReference type="STRING" id="15368.I1GZS4"/>
    <dbReference type="EnsemblPlants" id="KQK18989">
        <property type="protein sequence ID" value="KQK18989"/>
        <property type="gene ID" value="BRADI_1g45790v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846584"/>
    <dbReference type="Gramene" id="KQK18989">
        <property type="protein sequence ID" value="KQK18989"/>
        <property type="gene ID" value="BRADI_1g45790v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846584"/>
    <dbReference type="eggNOG" id="ENOG502S16H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058719_4_2_1"/>
    <dbReference type="OMA" id="TEWAFQN"/>
    <dbReference type="OrthoDB" id="1839683at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34052">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34052:SF1">
        <property type="entry name" value="OS06G0216700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094199" description="Phytocyanin domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="2">
        <location>
            <begin position="28"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK18989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="147" mass="16211" checksum="FE47D617089166BC" modified="2012-06-13" version="1">MEAKGGATTAVAVLLLLLVVVPEACRAERFVVGDAARWTWGYNYTDWVIRKGPFFQNDSLVFTYDPPNATTHAHSVYLMRSLAEYQSCNLKAAKLVAGVMQGAGSGYEFVLKKRKPHYFVCGERAGLHCTAGQMKFVVKPKSSACRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1H5L2</accession>
    <name>I1H5L2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100842374</name>
        <name evidence="7" type="ORF">BRADI_1g62760v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557935.1">
        <property type="nucleotide sequence ID" value="XM_003557887.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H5L2"/>
    <dbReference type="STRING" id="15368.I1H5L2"/>
    <dbReference type="MEROPS" id="C48.A02"/>
    <dbReference type="EnsemblPlants" id="KQK21735">
        <property type="protein sequence ID" value="KQK21735"/>
        <property type="gene ID" value="BRADI_1g62760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842374"/>
    <dbReference type="Gramene" id="KQK21735">
        <property type="protein sequence ID" value="KQK21735"/>
        <property type="gene ID" value="BRADI_1g62760v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842374"/>
    <dbReference type="eggNOG" id="KOG0778">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024324_6_1_1"/>
    <dbReference type="OMA" id="NEVHKCL"/>
    <dbReference type="OrthoDB" id="1939479at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.395.10:FF:000005">
        <property type="entry name" value="Ubiquitin-like-specific protease ESD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF95">
        <property type="entry name" value="OS03G0344300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="201"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK21735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="403" mass="47177" checksum="0DF4D71A0F7DEDFB" modified="2012-06-13" version="1">MISDAGLLFNRRKRRLPASDLLYPHFRSPKRKHFFFESPPIPPEARSLAFDMGNFLSSSLPDCMRRDSGLEVFRGWVRASQDARGLTVATSDDVELGQVVIRRFDPTKVARQAVPPPREKMKPFYKEALEKTGLHDKRLGEIEVEVTLQKEVLEELRKAPKEDLSQLFIPLTAEEENEVHDCLYGYGSSSEVLVLHESSNIEVSREKFRCLRPHGWLNDEVINLYLELLKERGIREPKRFLKCHFFNTFFYKKLAGGKNGYDYKSVKRWTTCRKLGYELIDCDKIFVPVHQSVHWCLAIINMKEKTFQYLDSLCGKDSRVRRVLDKYIADEVKDKSNKEIDISSWKEASLDYVPLQQNGWDCGMFMLKYIDFYSRGLSLSFGQEHMEYFRMRTVKEILRLRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6B6</accession>
    <name>I1H6B6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100840555</name>
        <name evidence="13" type="ORF">BRADI_1g64780v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558102.1">
        <property type="nucleotide sequence ID" value="XM_003558054.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6B6"/>
    <dbReference type="FunCoup" id="I1H6B6">
        <property type="interactions" value="383"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H6B6"/>
    <dbReference type="EnsemblPlants" id="KQK22047">
        <property type="protein sequence ID" value="KQK22047"/>
        <property type="gene ID" value="BRADI_1g64780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840555"/>
    <dbReference type="Gramene" id="KQK22047">
        <property type="protein sequence ID" value="KQK22047"/>
        <property type="gene ID" value="BRADI_1g64780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840555"/>
    <dbReference type="eggNOG" id="KOG0698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013173_3_1_1"/>
    <dbReference type="OMA" id="PRCCSEM"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000013">
        <property type="entry name" value="probable protein phosphatase 2C 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="12">
        <location>
            <begin position="26"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK22047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="433" mass="46283" checksum="52C1FC8B3E318D2C" modified="2012-06-13" version="1">MESASREERLPPALPLATLIGRELRGGGSERPLVRYGHSGFAKRGEDYFLVKPDCFRVPGDPSSAFSVFAVFDGHNGVSAAVFSKEHLLEDVMSAVPQGISREDWLQVLPRALVAGFVKTDIDFQRKGEMSGTTATLVVVDGFTVTVASVGDSRCILDTQGGVVSLLTVDHRLEENAEERERVTASGGEVSRLNLCGGQQVGPLRCWPGGLCLSRSIGDTDVGEFIVPIPHVKQVKLSNAGGRLIIASDGIWDAVSSETAAQACRGLPAELAAKLVVKQALKTSGLKDDTTCVVVDIIPSDHCSTPPPLSPNKNQNKLRSLLFGRRSHSSVGKLGNRKKSASFGFVEELFEEGSAKLEERLGRNSPSKAKFPPFRCAICQVDQVPFEDLMTDNGGGYCSAPSTPSAGPYLCSDCKKKKDAMEGKRSSPSTVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1H776</accession>
    <name>I1H776_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 9A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100836925</name>
        <name evidence="7" type="ORF">BRADI_1g67360v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228835.1">
        <property type="nucleotide sequence ID" value="XM_010230533.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H776"/>
    <dbReference type="STRING" id="15368.I1H776"/>
    <dbReference type="EnsemblPlants" id="KQK22463">
        <property type="protein sequence ID" value="KQK22463"/>
        <property type="gene ID" value="BRADI_1g67360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836925"/>
    <dbReference type="Gramene" id="KQK22463">
        <property type="protein sequence ID" value="KQK22463"/>
        <property type="gene ID" value="BRADI_1g67360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836925"/>
    <dbReference type="eggNOG" id="KOG2319">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036671_1_0_1"/>
    <dbReference type="OMA" id="FREYPYL"/>
    <dbReference type="OrthoDB" id="300289at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H776">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000325">
        <property type="term" value="C:plant-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.120:FF:000003">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.80:FF:000007">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041545">
        <property type="entry name" value="DUF5601"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18151">
        <property type="entry name" value="DUF5601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="6">
        <location>
            <begin position="111"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="439"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="439"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK22463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="494" mass="55217" checksum="81C0A38ED6FFB41D" modified="2012-06-13" version="1">MEGGGGGDAFGSSTAPLAWHDFLERMRQPSASEFVKSIKGFIVTFSNRAPDPERDSAAVQEFLENMEGAFRAHTPWAGSSEEELESAGEGLEKYVMTKLFNRVFASVPEDVKSDEELFEKMSLLQQFVRPENLDIKPEYQNETSWLLAQKELQKINMYKAPRDKLACILNCCKVINNLLMNASHMSNDNAPGADEFLPVLIYVTLKANPPQLHSNLLYIQRYRSQSRLVSEAQYFFTNILSAESFIWNIDAESLSMDERDFQKKMDLARERLLGLSVSSENQDNHTNLDVREHRSQILKASGNSDVHIYLKDHVQGPGHDIKRVSDVSSKPVERVQSISDLDKKGQSISDLEKKGATELLKDDDLSKIFQEYPFLFARAGDLTVADVGSLLNSYKQLVLRYVALSQGMGVNPEVPLVQSMHTASDVLASEEPENVSNVVNNSEISEGSSKTCDDIKNVNLDPEVGNVSTQQTAVDPRDDQKTLKAEASDKSEHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I1HA75</accession>
    <name>I1HA75_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104581893</name>
        <name evidence="3" type="ORF">BRADI_1g76600v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229270.1">
        <property type="nucleotide sequence ID" value="XM_010230968.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HA75"/>
    <dbReference type="FunCoup" id="I1HA75">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK23863">
        <property type="protein sequence ID" value="KQK23863"/>
        <property type="gene ID" value="BRADI_1g76600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581893"/>
    <dbReference type="Gramene" id="KQK23863">
        <property type="protein sequence ID" value="KQK23863"/>
        <property type="gene ID" value="BRADI_1g76600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104581893"/>
    <dbReference type="eggNOG" id="ENOG502S76W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154843_0_0_1"/>
    <dbReference type="OMA" id="FWTRTEH"/>
    <dbReference type="OrthoDB" id="783284at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK23863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="137" mass="14890" checksum="D4551A6B53EAED4C" modified="2012-06-13" version="1">MAALCPNRATALPFLPALRSPPRRLSPTAFVASVVRPRGPRIVLRGSRHPDLPARNSVWFENATMLRTEHKKKALFASETDSPTTDSSKRSDDNSSPPDGPPVLTILAGIIVFFLVLWVIGSIFTWIVGLVFGAAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1HB74</accession>
    <name>I1HB74_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100831114</name>
        <name evidence="4" type="ORF">BRADI_2g00750v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564889.1">
        <property type="nucleotide sequence ID" value="XM_003564841.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HB74"/>
    <dbReference type="STRING" id="15368.I1HB74"/>
    <dbReference type="EnsemblPlants" id="KQK02313">
        <property type="protein sequence ID" value="KQK02313"/>
        <property type="gene ID" value="BRADI_2g00750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831114"/>
    <dbReference type="Gramene" id="KQK02313">
        <property type="protein sequence ID" value="KQK02313"/>
        <property type="gene ID" value="BRADI_2g00750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831114"/>
    <dbReference type="eggNOG" id="ENOG502QQ61">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009340_3_0_1"/>
    <dbReference type="OMA" id="ACFDKFL"/>
    <dbReference type="OrthoDB" id="1602505at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HB74">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049172">
        <property type="entry name" value="DUF6857_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048297">
        <property type="entry name" value="DUF936_dom_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010341">
        <property type="entry name" value="DUF936_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31928:SF6">
        <property type="entry name" value="DUF936 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31928">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21647">
        <property type="entry name" value="DUF6857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06075">
        <property type="entry name" value="DUF936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF936" evidence="2">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6857" evidence="3">
        <location>
            <begin position="260"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="474"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK02313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="534" mass="56557" checksum="7C0B09B2C9182E6B" modified="2012-06-13" version="1">MTTLSTGVLLKLLDGMRSGAAKPVGEHRTAVLQVTDIVPADMDEKDLFPRHGQFYVKVSDSSHSIYATLPLAQADLVLTNKLSLGQFVHVDRLDPGSPVPVIVGARPQPGRHPLVVGTPEPPARGKAAVPRRGSWGPENHAIAASPKVAIKPTALNFDERTPVKATPARSSALSASVRKSSSVLPRLMTRSRSFAADRGDPPPKIPKSPFPAEKSSMSCTAASRPISRRVRVEEPSSPVSDDDTATSAKKRPSSAARVLVPGKLNLLGKEAMEQMEQAQKVALEALRNASATENVARIYKMFSELSKAARPDAPAACFESFLGFHQEAVQAVADIESIQAAMSMAASSAVAASDEPPAEEAHVLQEIAQNRTTAMRRRGGGLLGGLGGVSKSVSFAPGTLLDPSSRHDGSRSLSASRKCLAMDKTGGEGEKRSSSVLGSSLRLAKQMLAEAGSWFMEFLEAVLETGLKKKRSKQSSSSSSATGKQSGSGCSCPQSLMLRVINWVEMEQSGDSRKAGHPRAAAVARKLRIKAKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCM4</accession>
    <name>I1HCM4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100844985</name>
        <name evidence="2" type="ORF">BRADI_2g04990v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569754.1">
        <property type="nucleotide sequence ID" value="XM_003569706.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCM4"/>
    <dbReference type="STRING" id="15368.I1HCM4"/>
    <dbReference type="EnsemblPlants" id="KQK03008">
        <property type="protein sequence ID" value="KQK03008"/>
        <property type="gene ID" value="BRADI_2g04990v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844985"/>
    <dbReference type="Gramene" id="KQK03008">
        <property type="protein sequence ID" value="KQK03008"/>
        <property type="gene ID" value="BRADI_2g04990v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844985"/>
    <dbReference type="eggNOG" id="ENOG502SK9M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014546_2_0_1"/>
    <dbReference type="OMA" id="HFKAQYS"/>
    <dbReference type="OrthoDB" id="444127at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HCM4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF41">
        <property type="entry name" value="OS01G0179000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="438" mass="47014" checksum="02C16AB9776DF948" modified="2012-06-13" version="1">MAAAKKSVSRLAQRLVLPAEPTPSGPLRLSWLDRYPTQMALIESLHVFKPAPARHADACPGPARTIERALAQALVRYYPLAGRLAFTDDGGQSHVDCGGPRSGVWFTEAEAACGLEDVDYLEHPMMISKDELLPPTPAAEEGDERRLVLLVQVTSFACGGFVVGFRFSHAVADGPGAAQFMAAVGDLARGAESLSMEPQWGRDAVPDPAGAVVGALPDPAGAKRLEYLAMDISADYIDHFKAQYNSTNNGGARCSAFEVLVAKAWQSRTRAAGFDPSTTVHLCFAMNARPLLHASLPSAGAGFYGNCYYIMRVSAPAGKVSGSSVPEVVKIIKDGKRRMPAEFARWASGEAGAGGEDPYRITSDYRTLLVSDWTRLGFAEVDYGWGPPAHVVPLTNLDYIATCILVKPWAHKPGARLITQCVTPDRVAAFHQGMLDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1HFA9</accession>
    <name>I1HFA9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100835837</name>
        <name evidence="13" type="ORF">BRADI_2g12937v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567686.1">
        <property type="nucleotide sequence ID" value="XM_003567638.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HFA9"/>
    <dbReference type="FunCoup" id="I1HFA9">
        <property type="interactions" value="2505"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HFA9"/>
    <dbReference type="EnsemblPlants" id="KQK04317">
        <property type="protein sequence ID" value="KQK04317"/>
        <property type="gene ID" value="BRADI_2g12937v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835837"/>
    <dbReference type="Gramene" id="KQK04317">
        <property type="protein sequence ID" value="KQK04317"/>
        <property type="gene ID" value="BRADI_2g12937v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835837"/>
    <dbReference type="eggNOG" id="KOG0600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_78_0_1"/>
    <dbReference type="OMA" id="SSRHMEP"/>
    <dbReference type="OrthoDB" id="28397at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HFA9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07840">
        <property type="entry name" value="STKc_CDK9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000043">
        <property type="entry name" value="probable serine/threonine-protein kinase At1g54610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000021">
        <property type="entry name" value="probable serine/threonine-protein kinase At1g54610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF176">
        <property type="entry name" value="OS01G0367700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="91"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="412"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="481"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK04317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="548" mass="60980" checksum="645CB28D7386A889" modified="2012-06-13" version="1">MGCVFGRASSSATRKKEKASPKPAPSNGSPADGDKSLGRPRRRLGRRTGPRQGCVPAPAAAEQLAAGWPPWLVAVAGEALRGWTPRRADTFEKLNKIGSGTYSNVYRARDTVSGRIVALKKVRFDNLEPESVKFMAREILILRRLDHQNVIKLEGLVTSRMSCSLYLVFEYMEHDLAGLAASPEVKFTLPQIKCYMHQLLSGLEHCHDNNVLHRDIKGSNLLLDNNGVLKIADFGLAALFDPRHKRPMTSRVVTLWYRPPELLLGATDYGVGVDLWSAGCILAELLYGKPIMPGRTEVEQLHKIFKLCGSPSEEYWKKSKLPHATIFKPQQPYKRCIRDTFKDFPPSSLQLVETLLAIDPAERQTSTAALQSEFFASEPYACDPSSLPTYPPSKEMDAKLRDEEARRLRAAAKAKGEVAKRTRTRDRSHRAGPAPEANAELQTNLDQRRRMITHANAKSKSEKFPPPHQDGAMGNPLGSCRHMEPTYEHQDTSFSTVVPIEKGTSQTWSGPLFDPSALGQSRRKKQTALDAKAAAYSKQLRKEKGRAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2023-09-13" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJ55</accession>
    <name>I1HJ55_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g24435v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJ55"/>
    <dbReference type="EnsemblPlants" id="KQK06104">
        <property type="protein sequence ID" value="KQK06104"/>
        <property type="gene ID" value="BRADI_2g24435v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06104">
        <property type="protein sequence ID" value="KQK06104"/>
        <property type="gene ID" value="BRADI_2g24435v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2907179_0_0_1"/>
    <dbReference type="InParanoid" id="I1HJ55"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK06104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="62" mass="7193" checksum="002C0C8E53CA8FB1" modified="2012-06-13" version="1">MVLVTTKHGDFLRINMFLKENPWIPCTDRYTDKQQFFGPSYHSCPSPPPDLETVTISNFPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJT9</accession>
    <name>I1HJT9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cyclin-dependent kinase D-1</fullName>
            <ecNumber evidence="3">2.7.11.22</ecNumber>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">CDC2+/CDC28-related protein kinase R2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">CDK-activating kinase R2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100833383</name>
        <name evidence="22" type="ORF">BRADI_2g26510v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="3"/>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568531.1">
        <property type="nucleotide sequence ID" value="XM_003568483.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJT9"/>
    <dbReference type="FunCoup" id="I1HJT9">
        <property type="interactions" value="1770"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HJT9"/>
    <dbReference type="EnsemblPlants" id="KQK06467">
        <property type="protein sequence ID" value="KQK06467"/>
        <property type="gene ID" value="BRADI_2g26510v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833383"/>
    <dbReference type="Gramene" id="KQK06467">
        <property type="protein sequence ID" value="KQK06467"/>
        <property type="gene ID" value="BRADI_2g26510v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833383"/>
    <dbReference type="eggNOG" id="KOG0659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="OMA" id="GIHHCHR"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07841">
        <property type="entry name" value="STKc_CDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000289">
        <property type="entry name" value="Cyclin-dependent kinase D-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000097">
        <property type="entry name" value="Putative cyclin-dependent kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037770">
        <property type="entry name" value="CDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="18"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="311"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="24"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 18">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KQK06467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="419" mass="46938" checksum="81C66D5D88A9580F" modified="2012-06-13" version="1">MASGGDDDAGVKRVADRYLKREVLGEGTYGVVFKAVDTKTGSIVAIKRIRLGKYKEGVNFTALREIKLLKELKDPNIIELIDAFPYKGNLHLVFEFMETDLEAVIRDRNIVLSPADTKSYIQMMLKGLVVCHKKWVLHRDMKPNNLLIGAEGQLKLADFGLARIFGSPERNFTHQVFARWYRAPELLFGTKQYGSAVDIWAAGCIFAELLLRRPFLQGSSDIDQLGKIFAAFGTPKSSQWPDMVYLPDYVEYQFVSAPPLRSLFPMASDDALDLLSKMFTYDPKARITAQQALEHRYFSSVPAPTIPFKLPRPQPKGDSGNNKIPDLNLQGGPAVLSPPRKLRRVSAHEGMDRAEKAEEQPTGARRMDEMSSQSARIPMSVDVGVVFGTRPAPRPTLNSADKSRLKRKLDMDPEFGYAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1HTF1</accession>
    <name>I1HTF1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Laccase</fullName>
            <ecNumber evidence="5 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100826116</name>
        <name evidence="17" type="ORF">BRADI_2g55050v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Secreted</location>
            <location evidence="3 13">Extracellular space</location>
            <location evidence="3 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="5 13"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564616.1">
        <property type="nucleotide sequence ID" value="XM_003564568.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HTF1"/>
    <dbReference type="STRING" id="15368.I1HTF1"/>
    <dbReference type="EnsemblPlants" id="KQK10592">
        <property type="protein sequence ID" value="KQK10592"/>
        <property type="gene ID" value="BRADI_2g55050v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73207">
        <property type="protein sequence ID" value="PNT73207"/>
        <property type="gene ID" value="BRADI_2g55050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826116"/>
    <dbReference type="Gramene" id="KQK10592">
        <property type="protein sequence ID" value="KQK10592"/>
        <property type="gene ID" value="BRADI_2g55050v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73207">
        <property type="protein sequence ID" value="PNT73207"/>
        <property type="gene ID" value="BRADI_2g55050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826116"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006504_6_3_1"/>
    <dbReference type="OMA" id="WYHSHQV"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF109">
        <property type="entry name" value="LACCASE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="11 13" id="KW-0186">Copper</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982741" description="Laccase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="30"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="156"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="410"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK10592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="551" mass="58552" checksum="CF7760C26B146BAD" modified="2012-06-13" version="1">MAQLLWLASVLLLAASSVADAATANYTFTVASMRVNRLCNSTDIIAVNGQLPGPTIEVNDGDEVVVNVTNGSPYNLTIHWHGMLQLLTPWADGPSMVTQCPIQPNSSYAYRFNVTGQEGTLWWHAHSSFLRATVYGALIVKPRNGSAYPFPTPDQEVPLVLGEWWSQNVVDVEKDALMSGQLPSRSDAFTVNGLTGQLYPCANETFTVVVEPNTTVLLRVINAALNTHLFFKLAGHNFTVVAVDACYTANHTTDTLVLAPGNTVDALIFTGPKPAGSYYMAVQPHDTISPATMATSDDDSTATAILRYNGTSPTATPAMPAMPTSSDTSTANAFYFGLRGVKAFTAVPTKVDVNMTIELGLGQLPCDSAQTSCNGTAFAAAMNGVSFRLPTRVSLLEAQFKGKPAGVYTADFPDGPPGSGMAMVEGTKVRSLPYNSTVEIVLQNPTAVPAENHPIHLHGFNFFVLAQGLGTFTPGNASAYNLVDPVSRNTLAVPTGGWAVIRFVANNPGMWFFHCHLDAHVPMGLGMVFAVQNGTAPGSILPPPPADLPMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1HU77</accession>
    <name>I1HU77_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100838770</name>
        <name evidence="2" type="ORF">BRADI_2g57580v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564831.1">
        <property type="nucleotide sequence ID" value="XM_003564783.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HU77"/>
    <dbReference type="STRING" id="15368.I1HU77"/>
    <dbReference type="EnsemblPlants" id="KQK11009">
        <property type="protein sequence ID" value="KQK11009"/>
        <property type="gene ID" value="BRADI_2g57580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838770"/>
    <dbReference type="Gramene" id="KQK11009">
        <property type="protein sequence ID" value="KQK11009"/>
        <property type="gene ID" value="BRADI_2g57580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838770"/>
    <dbReference type="eggNOG" id="ENOG502SRKS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084340_0_0_1"/>
    <dbReference type="OMA" id="NSHYYTH"/>
    <dbReference type="OrthoDB" id="1284989at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34124">
        <property type="entry name" value="F16B3.27 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34124:SF2">
        <property type="entry name" value="F16B3.27 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK11009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="17078" checksum="8A2A924BDACF8591" modified="2012-06-13" version="1">MAKSHTHKAFLLCNYALLAAASSCIFLTLSLRLLPSPCGLLLVFLHALTAVFSAAGCSGSFTGPASWHTAHTAGAALTAIFQGAVALLAFTRTADFLAEIQSYVRDEDGAVILKMVGGLGTAIFVLEWAALALAFSLRLDDDEEDDDLNRTKNWAASSYHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYQ9</accession>
    <name>I1HYQ9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC104583471</name>
        <name evidence="4" type="ORF">BRADI_3g08050v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234039.1">
        <property type="nucleotide sequence ID" value="XM_010235737.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYQ9"/>
    <dbReference type="EnsemblPlants" id="KQJ94025">
        <property type="protein sequence ID" value="KQJ94025"/>
        <property type="gene ID" value="BRADI_3g08050v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66168">
        <property type="protein sequence ID" value="PNT66168"/>
        <property type="gene ID" value="BRADI_3g08050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583471"/>
    <dbReference type="Gramene" id="KQJ94025">
        <property type="protein sequence ID" value="KQJ94025"/>
        <property type="gene ID" value="BRADI_3g08050v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66168">
        <property type="protein sequence ID" value="PNT66168"/>
        <property type="gene ID" value="BRADI_3g08050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583471"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_0_1_1"/>
    <dbReference type="OMA" id="YSKCGCW"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000073">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000090">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015">
        <property type="entry name" value="OS07G0578800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015:SF930">
        <property type="entry name" value="PPR CONTAINING PLANT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="109"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="344"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="449"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ94025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="627" mass="69635" checksum="9330885FBFDC26F9" modified="2012-06-13" version="1">MSGKLSAAAVAPWKRLCKLVSDGRYQEALLAYSRAHASDLRPDAFTFPCLLKSCAALQDASAALQLHGNLVKSGFFSCPYTATALTSAYARLLRLTDARKVFDEMPERTVPCFNALIAGLSQCGKVDEARDVLSLLRKEGILPDSVTVASVLPACGVVEQAKQLHGLVVKTGDSLDRYVVTTLITKYLDYGDLETARRIFELVVHKGVESYNAMASGLSRNNEHFIALDTIREMGLGSSEKPNDTTLLVVLSACTSALASSLGKEAHCYVLKHAIDCNVKIKTALIDMYSKCGYLECAYQVFSNTEERNLVTWNTMISGFLIHDKLANALGLFEQLRLEGFNPDRITWNLMINGLAHHQKFFEVFSFFRKMRLEQVSGVNLETITSMLSACSAMSDIQHGKEIYCHVIRTMQDFEDDVFQTTVIDMFMSCGCDRYAGRVFGKEMRKSNDPALWNAMISGYGKCGKNCLALQTFNEMLKQQVQPNSATFLCALSACSHAGLVQKAMQLYQLMGSTYSVDPTIEHLSVILDLFCRAGKLPEAYDLLLKYANPPASMWYSFLGACRNYCNAELGEIAATKLYDLDPGSTTPWVILSNIYAEQYRWSEVETLRKMMSDRCLIKIPACSELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1I065</accession>
    <name>I1I065_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxisomal membrane protein PMP22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100843682</name>
        <name evidence="7" type="ORF">BRADI_3g12810v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573232.1">
        <property type="nucleotide sequence ID" value="XM_003573184.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I065"/>
    <dbReference type="FunCoup" id="I1I065">
        <property type="interactions" value="591"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I065"/>
    <dbReference type="EnsemblPlants" id="KQJ94720">
        <property type="protein sequence ID" value="KQJ94720"/>
        <property type="gene ID" value="BRADI_3g12810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843682"/>
    <dbReference type="Gramene" id="KQJ94720">
        <property type="protein sequence ID" value="KQJ94720"/>
        <property type="gene ID" value="BRADI_3g12810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843682"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="QHSVFAY"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I065">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF46">
        <property type="entry name" value="OS08G0566900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ94720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20757" checksum="3A9B7655B939CE4F" modified="2012-06-13" version="1">MAAMAGEAYMKQLRAHPLRTKAITSGVLAGCSDAVAQKISGVKKLQLRRLLLIMLYGFAYAGPFGHFFHKLMDRIFKGKKGKETTAKKVIVEQLTVSPWNNMMFMMYYGLVVEGRPFTQVKSKVKKDYATIQLTAWKFWPIVSWINYEYMPLQLRVLFASSVASCWAVFLNLKAARSSSIVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1I223</accession>
    <name>I1I223_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome non-ATPase regulatory subunit 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100835233</name>
        <name evidence="14" type="ORF">BRADI_3g18280v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the 19S regulatory particle (RP/PA700) base subcomplex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The RP/PA700 complex is composed of at least 17 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the proteasome subunit S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571547.1">
        <property type="nucleotide sequence ID" value="XM_003571499.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I223"/>
    <dbReference type="FunCoup" id="I1I223">
        <property type="interactions" value="2981"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I223"/>
    <dbReference type="EnsemblPlants" id="KQJ95640">
        <property type="protein sequence ID" value="KQJ95640"/>
        <property type="gene ID" value="BRADI_3g18280v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835233"/>
    <dbReference type="Gramene" id="KQJ95640">
        <property type="protein sequence ID" value="KQJ95640"/>
        <property type="gene ID" value="BRADI_3g18280v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835233"/>
    <dbReference type="eggNOG" id="KOG2062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002323_0_0_1"/>
    <dbReference type="OMA" id="NKVAHAD"/>
    <dbReference type="OrthoDB" id="261572at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000048">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016642">
        <property type="entry name" value="26S_Psome_Rpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048570">
        <property type="entry name" value="PSMD1_RPN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040623">
        <property type="entry name" value="RPN2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF13">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18004">
        <property type="entry name" value="RPN2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21505">
        <property type="entry name" value="RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015947">
        <property type="entry name" value="26S_Psome_Rpn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal" evidence="13">
        <location>
            <begin position="8"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome regulatory subunit RPN2 C-terminal" evidence="12">
        <location>
            <begin position="793"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="852"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="964"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="852"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="964"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ95640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="994" mass="108057" checksum="E267BB5FAA8F45C1" modified="2012-06-13" version="1">MAAATVSSASGLLAMLQEPAAELKLHALSNLNSLVHVFWPEISTSVPAIESLYEDEEFDQRHLAALVVSKVFYYLSELNDSLSYALGAGPLFDVSEDSDYTNTLLARALDEYASIRSKTSKATEEEEMMDPRLEVIVERMLDKCILDGKYQQAMGMAVECRRLDKLEDAISRCDNLHGALSYCINLSHQYVSHREYRCEILRCLVKIYKTLSNPDYLSICQCLMFLGESETVADILHKLLSGSEDDALLAYQIAFDLVENENQAFLLNVRNHLDTLSMRTSALPSGQTDNAATEPAGDIQMGDDVATSNGSAQVVDPNEVAVTDKLSKIKGILSGETSIQLTLQFLYSHNRSDLLILKTIKQALEMRNSVCHSATICSNAIMHAGTTVDTFLRENLEWLSRATNWAKFSATAGLGVIHRGHLQQGRALMAPYLPQGGAVGGGSPYSEGGALYALGLIHANHGEGIKQFLRESLRTTSSEVVQHGACLGLGLASLGTADEEIFEDIKNILYTDSAVASEAAGIGLGLLMVGTATEKAGEMLAYAHDTQHEKIIRGLALGIALTVYGREEEADTLIEQMTRDQDPILRYGGMYAIALAYRGTANNKAIHQLLHFAVSDVNDDVRRTAVLALGFVLYNEPEQTPRIVSLLSESYNPHVRYGAALAVGISCAGTGLSDAISLLEPLTSDVVDFVRQGALIAMAMVMIQTNESYDPRVGTFRRQLEKIILDKHEDTMSKMGAILASGIIDAGGRNVTIKLKSKSKHDKLTAVVGLAVFSQFWYWYPLTYFISLAFSPTAIIGVNSDLRMPKLEFLSNAKPSLFDYPKPTSQQTATAAVQLPTAILSTYAKAKSRAAKKDAESKAQEKAEAVQEKAETAEGKAEAPTSEDASTSMQVDGAAPEKKAPEPEPTFQVLANPARVLPAQEKFIKFLEGSRYEPVKMAPSGFILLRDLKPTEAEELVLADTPSTAAAATNATSQQSAMAVDEEPQPPEPFEYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1I247</accession>
    <name>I1I247_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_3g18490v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_3g18490v3"/>
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        <property type="protein sequence ID" value="PNT66915"/>
        <property type="gene ID" value="BRADI_3g18490v3"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="term" value="F:histone binding"/>
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        <property type="term" value="P:chromatin remodeling"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_PAC1"/>
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        <property type="entry name" value="WD40_repeat_CS"/>
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        <property type="entry name" value="WD40_rpt"/>
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        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
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        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="GPROTEINBRPT"/>
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        <property type="match status" value="6"/>
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    </dbReference>
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        <location>
            <begin position="86"/>
            <end position="128"/>
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        <location>
            <begin position="182"/>
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    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        </source>
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            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="345" mass="39391" checksum="ADFACFFEB4B220EF" modified="2012-06-13" version="1">MNTPKLQFLGNIAHKKVYKSRIADMAMVQIVQEINHDGPVYRARYMPQNSFIIATKTDSGEVYVFDYRKHPAKPPLNGACNPDLRLKGHNSEGYGLSWSIFKEGHLLSGSDDVKICLWDIKANGKNKTLDAYQTFKFYHHGNVEDVAWHLRHGYLFGSVGEDRNLLIWDLRSSASSKPVQSVEEHQSEVNCLAFNPLNEWVVATGSTDKTVKLFDLRKIDTSLHTLNFHKEGIVQLGWCPKNEAVLASSCLGKKLMVWDLSRIGREQSPEDAEEGPPELLFIHGGHKRNISDFSWNQCEDWMLASVDEENILHVWQIAQHVYDDDHEDDFPGDNRPAFQKLFETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2A2</accession>
    <name>I1I2A2_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="3" type="primary">LOC104583582</name>
        <name evidence="2" type="ORF">BRADI_3g18900v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234514.1">
        <property type="nucleotide sequence ID" value="XM_010236212.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2A2"/>
    <dbReference type="EnsemblPlants" id="KQJ95768">
        <property type="protein sequence ID" value="KQJ95768"/>
        <property type="gene ID" value="BRADI_3g18900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583582"/>
    <dbReference type="Gramene" id="KQJ95768">
        <property type="protein sequence ID" value="KQJ95768"/>
        <property type="gene ID" value="BRADI_3g18900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583582"/>
    <dbReference type="eggNOG" id="ENOG502S2C5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097715_0_0_1"/>
    <dbReference type="OMA" id="AAYEHRC"/>
    <dbReference type="OrthoDB" id="752671at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="entry name" value="OS08G0243800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS08G0243800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ95768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="174" mass="18846" checksum="D0E3F333BEB0EB6A" modified="2012-06-13" version="1">MGIGSRRRSAASRSVADLSTAAHEHRYPADEDDFEFEFTPLLPHGRRDHGSDTRATPSRTASHGSGTRASTLQAPRAERAPASASARRQESGKARGRRPVRWHELAFGSARVPAAMDMAEIRRRLRRRDGDIELAAAEAEAWAPWRLIRSLSCRGVEAVAASAAASASAPVRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1I380</accession>
    <name>I1I380_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100830036</name>
        <name evidence="9" type="ORF">BRADI_3g22020v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573780.1">
        <property type="nucleotide sequence ID" value="XM_003573732.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234623.1">
        <property type="nucleotide sequence ID" value="XM_010236321.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I380"/>
    <dbReference type="STRING" id="15368.I1I380"/>
    <dbReference type="EnsemblPlants" id="KQJ96254">
        <property type="protein sequence ID" value="KQJ96254"/>
        <property type="gene ID" value="BRADI_3g22020v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ96257">
        <property type="protein sequence ID" value="KQJ96257"/>
        <property type="gene ID" value="BRADI_3g22020v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830036"/>
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        <property type="protein sequence ID" value="KQJ96254"/>
        <property type="gene ID" value="BRADI_3g22020v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96257">
        <property type="protein sequence ID" value="KQJ96257"/>
        <property type="gene ID" value="BRADI_3g22020v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830036"/>
    <dbReference type="eggNOG" id="KOG3030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="WYHAPAG"/>
    <dbReference type="OrthoDB" id="10030083at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I380">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000001">
        <property type="entry name" value="Lipid phosphate phosphatase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF35">
        <property type="entry name" value="RE23632P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="101"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ96254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="310" mass="34725" checksum="7FE92E42F47D21B8" modified="2012-06-13" version="1">MADIQLGCHTVRSHGAKVARLHMYDWIILFFLAVVDGLLNIIEPFHRFVGRDMMTDLSYPLKGNTIPFWAVPLFAVVLPWIIFVGIYFKKKNVYDLHHGILGILYSVLITAVITDAIKDAVGRPRPDFFWRCFPDGKPNYDKFTSNVICHGEKSVIKEGHKSFPSGHSSGSFAGLGFLAWYLAGKLAAFDRKGHIAKLCLVFLPLLVASLVAVSRVDDYWHHWQDVFAGGIIGLTVASFCYLQFFPYPFDADAIWPHAYFQQLAETQSNGIANSYDMRPTGVEPVDEGHGAIALRNTSPMLDAMESGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1I4E6</accession>
    <name>I1I4E6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100822877</name>
        <name evidence="11" type="ORF">BRADI_3g27890v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Intracellular vesicle trafficking and protein transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574002.1">
        <property type="nucleotide sequence ID" value="XM_003573954.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I4E6"/>
    <dbReference type="STRING" id="15368.I1I4E6"/>
    <dbReference type="EnsemblPlants" id="KQJ96947">
        <property type="protein sequence ID" value="KQJ96947"/>
        <property type="gene ID" value="BRADI_3g27890v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67481">
        <property type="protein sequence ID" value="PNT67481"/>
        <property type="gene ID" value="BRADI_3g27890v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822877"/>
    <dbReference type="Gramene" id="KQJ96947">
        <property type="protein sequence ID" value="KQJ96947"/>
        <property type="gene ID" value="BRADI_3g27890v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67481">
        <property type="protein sequence ID" value="PNT67481"/>
        <property type="gene ID" value="BRADI_3g27890v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822877"/>
    <dbReference type="eggNOG" id="KOG0080">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_1_1"/>
    <dbReference type="OMA" id="YSTNRDC"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01863">
        <property type="entry name" value="Rab18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000456">
        <property type="entry name" value="Ras-related protein RABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ96947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="217" mass="23701" checksum="DA6DB7D3F5C6326D" modified="2012-06-13" version="1">MGLSPGTAGGSSAYECSFKILLIGDSGVGKSSLLVSFVAAATLDDDITPTIGVDFKIKFLTVGGKKLKLTIWDTAGQERFRTITGSYYRGAQGIILVYDVAKRESFTNLADVWTKEIDSNSSNKDCIKMLVGNKVDKDDERTVTREEGLAFAEESGCLFLESSAKTRENVENCFEQLTLKILEVPSLLEEGCSSVVKRNILKQQQESHAKYGGRCCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1I4P1</accession>
    <name>I1I4P1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100824400</name>
        <name evidence="5" type="ORF">BRADI_3g28747v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234820.1">
        <property type="nucleotide sequence ID" value="XM_010236518.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I4P1"/>
    <dbReference type="EnsemblPlants" id="KQJ97087">
        <property type="protein sequence ID" value="KQJ97087"/>
        <property type="gene ID" value="BRADI_3g28747v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67540">
        <property type="protein sequence ID" value="PNT67540"/>
        <property type="gene ID" value="BRADI_3g28747v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824400"/>
    <dbReference type="Gramene" id="KQJ97087">
        <property type="protein sequence ID" value="KQJ97087"/>
        <property type="gene ID" value="BRADI_3g28747v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67540">
        <property type="protein sequence ID" value="PNT67540"/>
        <property type="gene ID" value="BRADI_3g28747v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824400"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_49_0_1"/>
    <dbReference type="OMA" id="DTISCNM"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF28">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="175"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="210"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="245"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="315"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="350"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="385"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="420"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="455"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="490"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ97087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="603" mass="67069" checksum="9EAB4C261895351C" modified="2012-06-13" version="1">MTHSGAVSCHRYGLWASGSAPLIVISASMMQSSRVRAHAVHLDCPPRTEQKNGGSLVVMRPYQSPRSQDDFAKRNNEGNVVVMRPDRRLWDGLVGDAATGNSRPAADIEKAVLSLQAKPWKRLGQYSDEGVDGVHLPKDKGMLYLGNLRRYCNNGKLIQACCVIDEMVLHGQIPDSKCCIRLIRGLVKTGKANKARQVLEVMVLSGGIPDTITCNMLIAQLCRGGQLSSAMSVLEDMRYSGTSPNGITFNTLIRCMCNQHMYDKAITFWKEQLRIGWPPYVMTNTLLVDLICKKCGSKRAMEVLDELSLEGCQPDVVTYNALISASCKADRLKDAKTILTRLIAEGLEPNSTTYCILLHSLCNKRRWAEVCDLLAHMKRANCEPDVTAYNIFINYFCKYGHLDQAIDVLEKMVSDKCCPDIVTYNTLLNAISKEGMVEEALVIAHFIRQNGWQLVLITYNTLIDALANKGEVKKAMVLFDEMASGRISPDDITYGSLVMGFCKKNMSEEALELLNRTLALGFQVKTTTFIMVIQALCRDGKVEAAAEILRVMVSETNNTNSSFYLSIVRRVAKSGRIEEAERLHQELIDCKILKKDSLVVLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1I4S9</accession>
    <name>I1I4S9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flotillin-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100832661</name>
        <name evidence="6" type="ORF">BRADI_3g29050v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <location evidence="4">Caveola</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the band 7/mec-2 family. Flotillin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571892.1">
        <property type="nucleotide sequence ID" value="XM_003571844.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I4S9"/>
    <dbReference type="STRING" id="15368.I1I4S9"/>
    <dbReference type="EnsemblPlants" id="KQJ97144">
        <property type="protein sequence ID" value="KQJ97144"/>
        <property type="gene ID" value="BRADI_3g29050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832661"/>
    <dbReference type="Gramene" id="KQJ97144">
        <property type="protein sequence ID" value="KQJ97144"/>
        <property type="gene ID" value="BRADI_3g29050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832661"/>
    <dbReference type="eggNOG" id="KOG2668">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030844_0_0_1"/>
    <dbReference type="OMA" id="VEYVTSM"/>
    <dbReference type="OrthoDB" id="6080404at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044853">
        <property type="term" value="C:plasma membrane raft"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03399">
        <property type="entry name" value="SPFH_flotillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027705">
        <property type="entry name" value="Flotillin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806:SF23">
        <property type="entry name" value="FLOTILLIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806">
        <property type="entry name" value="FLOTILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="5">
        <location>
            <begin position="34"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ97144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="496" mass="54192" checksum="CFC4DC3D5421D1C2" modified="2012-06-13" version="1">MARFHVADASQYLAITGWGIDDVKLAKKALVFVGQDCKKFSISPVNYEFEVHAMSAEKLPFVLPAVFTIGPKVTPAITDPPAKGIQEMEQQLLLYAKLIAPLHNSDTKVHDLVKGVIEGETRVLAAELTMEEIFKGTKTFKKQVFERVQLELNQFGLIIYNANVKQLVDVPGHEYFSYLGQKTQQDAANQAKVDVAEAKMKGEVGAKERDGLTRQNAAKVDAETKVLSVRQQGQGLKEEAKVKAEVKVFENAREAEVAAAMADLKMKKAGWDKQAKVAEVEAAKAVAIREAELQMEVERKNALRQTEKLKAEQLSKATVQYETGVQDSNALLYSRQKAAEAALFEQMRAAEARKAQADAQFFEQKMSEDAKLYAKQKEAESLALVGKAKTEYVASMLQALGGNYHALRDYLMIDGGMYAEMARINAGAVNGMQPKISIWSNGEGAGGSEAAAGNALQQVAGVYKMLPPLLSTVHEQTGMLPPAWIGTLPKDKAAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1I4X2</accession>
    <name>I1I4X2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100841854</name>
        <name evidence="3" type="ORF">BRADI_3g29430v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571916.1">
        <property type="nucleotide sequence ID" value="XM_003571868.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I4X2"/>
    <dbReference type="FunCoup" id="I1I4X2">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ97207">
        <property type="protein sequence ID" value="KQJ97207"/>
        <property type="gene ID" value="BRADI_3g29430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841854"/>
    <dbReference type="Gramene" id="KQJ97207">
        <property type="protein sequence ID" value="KQJ97207"/>
        <property type="gene ID" value="BRADI_3g29430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841854"/>
    <dbReference type="eggNOG" id="ENOG502SYET">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044274_0_0_1"/>
    <dbReference type="OMA" id="HEHLRIQ"/>
    <dbReference type="OrthoDB" id="687745at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33098">
        <property type="entry name" value="COTTON FIBER (DUF761)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33098:SF44">
        <property type="entry name" value="DUF4408 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ97207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="31415" checksum="0AFA41194A76B22A" modified="2012-06-13" version="1">MNCSILKAAAVAGAAAVLLAATASAAGSYAVSAAPLVIPSGFLSSPHFLWVAANAIVLWLIVSSYRHHRTTGAAVSSDMDGAGLLFPSPEHENHAFAAAPVVVSLSPREARAAAKQRLAGDRPRARKKKPACEGTPPAKAEAPVGEAMATSGAVVVADDVSMDAAWQSILRRGVARPVAVRKSETWGGEELPRMRRAADRAVVGVAERRTGEMRKSASMVPPSPPHPAAASSPVAAKQGWRTRDVLGMAQDELLRRAESFIRRQHEHLRIQRQESEQRQALVEHERRRPAQIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5H7</accession>
    <name>I1I5H7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100836268</name>
        <name evidence="9" type="ORF">BRADI_3g31430v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXA1/ALB3/YidC (TC 2.A.9.2) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574213.1">
        <property type="nucleotide sequence ID" value="XM_003574165.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5H7"/>
    <dbReference type="STRING" id="15368.I1I5H7"/>
    <dbReference type="EnsemblPlants" id="KQJ97497">
        <property type="protein sequence ID" value="KQJ97497"/>
        <property type="gene ID" value="BRADI_3g31430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836268"/>
    <dbReference type="Gramene" id="KQJ97497">
        <property type="protein sequence ID" value="KQJ97497"/>
        <property type="gene ID" value="BRADI_3g31430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836268"/>
    <dbReference type="eggNOG" id="KOG1239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029282_4_0_1"/>
    <dbReference type="OMA" id="FSRGNRH"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I5H7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF69">
        <property type="entry name" value="MEMBRANE PROTEIN OXA1-LIKE, MITOCHONDRIAL, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="8">
        <location>
            <begin position="154"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ97497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="423" mass="45869" checksum="0843D5D647D68718" modified="2012-06-13" version="1">MAFAARRSLASRFSHHLTRRLHPSVPHLLSSRSNDDDRPSPSKPPQLPPFRSPLPPASRAAQTLNHLLPFSLHHSSLPRRNFSSSAPAPASAPAPTGEADAAAGVLADAAEAAAATVPAPFPGEVAAAAADSFFPVAALQHLIDTIHTFTGLNWWACIALTTVLIRSATIPLLVNQLKSTHKLNAIKPEMEAIKDEMNSMDQKSAMEGRKKIKALFKKHGVSPFSPLKGLLIQGPMFMSFYFAINNMVEKVPSLKGGGAFWFTDLTTPDPLYILPVLTGLTFLATVEINLQEGMETNPMAGKMKMFSRGMAVMTVPFTMNFAKGIFCYWITSNLFTLVYGIVIRRPTVRKLFNLHALVAPSAPGQKSAFSFFGGSKAIPSADSPLAIMSAKQSLEKPDAAALGYRVKNLDKKVKSRGKSRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1I7M3</accession>
    <name>I1I7M3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100821631</name>
        <name evidence="10" type="ORF">BRADI_3g37570v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574583.1">
        <property type="nucleotide sequence ID" value="XM_003574535.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I7M3"/>
    <dbReference type="FunCoup" id="I1I7M3">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I7M3"/>
    <dbReference type="EnsemblPlants" id="KQJ98548">
        <property type="protein sequence ID" value="KQJ98548"/>
        <property type="gene ID" value="BRADI_3g37570v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821631"/>
    <dbReference type="Gramene" id="KQJ98548">
        <property type="protein sequence ID" value="KQJ98548"/>
        <property type="gene ID" value="BRADI_3g37570v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821631"/>
    <dbReference type="eggNOG" id="KOG0374">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_0_0_1"/>
    <dbReference type="OMA" id="REDYCHI"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000026">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF416">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="119"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ98548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="305" mass="34424" checksum="F556C8E18A995666" modified="2012-06-13" version="1">MDGNALEELIRRLLDGKKNKGPGKKVQISEAEIRHLCVTAKEIFLSQPNLLELEAPINVCGDIHGQFSDLLRLFDYGGLPPSANYLFLGDYVDRGKQSIETICLLLAYKIKFPDNFFLLRGNHECASINRIYGFYDECKRRFSVRLWKLFTDCFNCLPVAAVIDDKILCMHGGLSPDLDSLDRIREIQRPVDVPDQGLLCDLLWSDPDRESSGWGENDRGVSFTFGADKVAEFLNKHDLDLICRAHQVVEDGYEFFADRQLVTIFSAPNYCGEFNNAGALMNVDASLLCSFQILKPLRGRSQTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1I9X8</accession>
    <name>I1I9X8_BRADI</name>
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            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">BRADI_3g44100v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575011.1">
        <property type="nucleotide sequence ID" value="XM_003574963.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I9X8"/>
    <dbReference type="STRING" id="15368.I1I9X8"/>
    <dbReference type="EnsemblPlants" id="KQJ99586">
        <property type="protein sequence ID" value="KQJ99586"/>
        <property type="gene ID" value="BRADI_3g44100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826468"/>
    <dbReference type="Gramene" id="KQJ99586">
        <property type="protein sequence ID" value="KQJ99586"/>
        <property type="gene ID" value="BRADI_3g44100v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826468"/>
    <dbReference type="eggNOG" id="ENOG502S42P">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="OrthoDB" id="782808at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="78"/>
            <end position="99"/>
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    </feature>
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        <location>
            <begin position="78"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ99586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="197" mass="21857" checksum="754723AA31369B3F" modified="2012-06-13" version="1">MAMATGGAVGEDKGRRKKVELLQEAIDGLLQEKRGKPQQHQQQREGEDASSTMVLRDQEDDLFLSSLLSKLDGVEKDVDLDHDEPNSFHPKPESSNEVGLGDIAKDLNKIKRQNMITHILLGTVIVMTAVWQFNEVSFLLRVQEKLSNPFKYLGDMIKSSLKRDRKASIEASPLPPVGVPDVTRADLPMLVIGNDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
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    <name>I1IAX8_BRADI</name>
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100841453</name>
        <name evidence="3" type="ORF">BRADI_3g46987v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003575183.4"/>
    </dbReference>
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        <property type="interactions" value="1843"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IAX8"/>
    <dbReference type="EnsemblPlants" id="KQK00048">
        <property type="protein sequence ID" value="KQK00048"/>
        <property type="gene ID" value="BRADI_3g46987v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841453"/>
    <dbReference type="Gramene" id="KQK00048">
        <property type="protein sequence ID" value="KQK00048"/>
        <property type="gene ID" value="BRADI_3g46987v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841453"/>
    <dbReference type="eggNOG" id="ENOG502QRQ3">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032694_0_0_1"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34778">
        <property type="entry name" value="OS02G0580700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34778:SF2">
        <property type="entry name" value="OS02G0580700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="242"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="KQK00048.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK00048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="652" mass="71800" checksum="742E2E7E0D143788" modified="2012-06-13" version="1">MAESEVEALRKAYAEIMLNTTKESAARVLAAEERAAVLEGRVAAAKEDGVAALVRLKAIMEARIKEVESKSLAHVRKINELQEQLHGAQNTVASLQVELQTANTELEQTRTTLAEERNNILPTCDEMDSNKNRSSCSKMHVQSRSVSLKNKDTADDICCVPTSTESGAAENRENVCGTDVSSIARNKKSKLYRYGCTQRIRALKRRSPRADSSEQNGMQASVLNIRSKMGKNNTARNTRRTRSIMEQILETEFQGNCKRKRGQRRRPSYKHDSSEVHGKTEDTSSDASDKDGCLQLLKALEQDLSPPNMFTRHSGEGLTDRKDYLPIGGKDAPALIEAPALSKMQIVRRKRTKTVRISDDGYSLSKSAPGNTLRTSTNKDTIFKSEQTSESIDNHSDTPASKNVSGLSVGTENLMHPSDGTENLMYPSDDTENLKQPSDATENSMHPSDHIENLMQLSDTTESLMHPNIASTGQFESGNSSPLVLQSTKRQIDSEGELRVDHPKCRTPETNSAGWQEVKVDESCNLALDRSGPSAIISLDKEQNAMASGVPVQPGGARRIQYTFNRRKRKSMSLDSTPQGAIPEKGSSLVNLTDKQESQALPGKSSNLVNLADKQESQALPETQNLLVESPQDDSELVHVAQQLVLLSELKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBC7</accession>
    <name>I1IBC7_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="15368.I1IBC7"/>
    <dbReference type="EnsemblPlants" id="KQK00238">
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        <property type="gene ID" value="BRADI_3g48110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00238">
        <property type="protein sequence ID" value="KQK00238"/>
        <property type="gene ID" value="BRADI_3g48110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838617"/>
    <dbReference type="eggNOG" id="ENOG502S3X3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147134_0_0_1"/>
    <dbReference type="OMA" id="AHHEITS"/>
    <dbReference type="OrthoDB" id="775892at2759"/>
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        <property type="component" value="Chromosome 3"/>
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    <dbReference type="ExpressionAtlas" id="I1IBC7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36709">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36709:SF1">
        <property type="entry name" value="OS02G0604100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="42"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK00238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="128" mass="14685" checksum="9858535A0007BA65" modified="2012-06-13" version="1">MAKYNVVQKNKREFNQDRKRQAHGDPNSGKLKHRNAPVSMSGKRKRKLLRRHNRDQKEAVMVKALENNMGDVEMASAEASLETAKDKSQMKFNVKNSRIQIKRLKGKGRNKAKNAKQPTKEKADAMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IE97</accession>
    <name>I1IE97_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H/ACA ribonucleoprotein complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucleolar protein family A member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100824602</name>
        <name evidence="10" type="ORF">BRADI_3g56240v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570455.1">
        <property type="nucleotide sequence ID" value="XM_003570407.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IE97"/>
    <dbReference type="STRING" id="15368.I1IE97"/>
    <dbReference type="EnsemblPlants" id="KQK01502">
        <property type="protein sequence ID" value="KQK01502"/>
        <property type="gene ID" value="BRADI_3g56240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824602"/>
    <dbReference type="Gramene" id="KQK01502">
        <property type="protein sequence ID" value="KQK01502"/>
        <property type="gene ID" value="BRADI_3g56240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824602"/>
    <dbReference type="eggNOG" id="KOG3167">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084513_1_1_1"/>
    <dbReference type="OMA" id="EDNYEAR"/>
    <dbReference type="OrthoDB" id="5364946at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IE97">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034513">
        <property type="term" value="F:box H/ACA snoRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000015">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit NHP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="9">
        <location>
            <begin position="33"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK01502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="150" mass="16331" checksum="31EB4BA9A0A80E64" modified="2012-06-13" version="1">MGSDTETEKKRTPVALAPIAKPLAGKKLSKRTLKLVRRASEAKCLKRGVKEVVKSIRRGSKGLCVIAGNISPIDVITHLPILCEEANVPYIYVTSKEELATAGTTKRPTCCVLVMTKPAKGEISEEVKEKLQSDYDQVVTEVAEVTSSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEZ3</accession>
    <name>I1IEZ3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyanobacterial aminoacyl-tRNA synthetase CAAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100836567</name>
        <name evidence="5" type="ORF">BRADI_3g58290v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573067.1">
        <property type="nucleotide sequence ID" value="XM_003573019.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEZ3"/>
    <dbReference type="STRING" id="15368.I1IEZ3"/>
    <dbReference type="EnsemblPlants" id="KQK01795">
        <property type="protein sequence ID" value="KQK01795"/>
        <property type="gene ID" value="BRADI_3g58290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836567"/>
    <dbReference type="Gramene" id="KQK01795">
        <property type="protein sequence ID" value="KQK01795"/>
        <property type="gene ID" value="BRADI_3g58290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836567"/>
    <dbReference type="eggNOG" id="ENOG502S1WK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095488_1_0_1"/>
    <dbReference type="OMA" id="YSIWFTS"/>
    <dbReference type="OrthoDB" id="2014299at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025564">
        <property type="entry name" value="CAAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033344">
        <property type="entry name" value="CURT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222:SF2">
        <property type="entry name" value="PROTEIN CURVATURE THYLAKOID 1D, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14159">
        <property type="entry name" value="CAAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Cyanobacterial aminoacyl-tRNA synthetase CAAD" evidence="4">
        <location>
            <begin position="119"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK01795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="190" mass="19654" checksum="5B264EE6627019A7" modified="2012-06-13" version="1">MEVRVSTAAACSAPLSIARGRRPPPSVAVVRGPARGVRCRSAAPDPVPSEEPSSASASTVVAVTDKPAGEEKGEEAGAVSGGSSGAVEATVADAPASSGTPEEDGSVDDILSKLDIEVTPTYVLFGSGALIALLILSKVVAAIDSVPLLPKVLELVGTGYSIWFTTRYLLFKESRDELFAKFEDLKERII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IG28</accession>
    <name>I1IG28_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Purple acid phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100821451</name>
        <name evidence="12" type="ORF">BRADI_4g00590v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="8"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578743.2">
        <property type="nucleotide sequence ID" value="XM_003578695.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IG28"/>
    <dbReference type="FunCoup" id="I1IG28">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ85612">
        <property type="protein sequence ID" value="KQJ85612"/>
        <property type="gene ID" value="BRADI_4g00590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821451"/>
    <dbReference type="Gramene" id="KQJ85612">
        <property type="protein sequence ID" value="KQJ85612"/>
        <property type="gene ID" value="BRADI_4g00590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821451"/>
    <dbReference type="eggNOG" id="KOG1378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013387_0_1_1"/>
    <dbReference type="OMA" id="FICISTE"/>
    <dbReference type="OrthoDB" id="45007at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000034">
        <property type="entry name" value="Fe(3+)-Zn(2+) purple acid phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.380">
        <property type="entry name" value="Purple acid phosphatase-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039331">
        <property type="entry name" value="PAPs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015914">
        <property type="entry name" value="PAPs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008963">
        <property type="entry name" value="Purple_acid_Pase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953">
        <property type="entry name" value="ACID PHOSPHATASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953:SF111">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49363">
        <property type="entry name" value="Purple acid phosphatase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982811" description="Purple acid phosphatase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase N-terminal" evidence="11">
        <location>
            <begin position="54"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="9">
        <location>
            <begin position="157"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="10">
        <location>
            <begin position="381"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ85612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="459" mass="51908" checksum="85887594654D0CD4" modified="2012-06-13" version="1">MEPHMLTLFLLLIAALLAHAGAGITSSYRRSLLGLSAMPFDADVFRPPPGYNAPEQVHITQGDLTGRAMTVSWVTPHHPGSNVVRYGLAADNLTRFAEGTVRRYAFGGSYQSGHIHHATLSGLDHATVYHYAVGYGYENVRRFSFKTPPAPGPETTIRFGVIGDLGQTAHSNDTLAHYEARPGDAVLFIGDLSYADNHPAHDNRRWDSWARFVERNVAYQPWIWTTGNHEIDFAPEIGETVPFKPFTNRYRTPFRASNSTEPFFYSVKMGPAHVIMLSSYTSYGKYTPQWTWLQDELTTRVDRNVTPWLIICVHSPWYNTNEYHYMEGETMRVQFERWVVDAKADIVFAGHVHSYERTHRVSNVAYDIANGKATPAFNVSAPVYVTIGDGGNIEGLATTFRSPQPDYSAFREASFGHATLEIMNKTHAYYEWHRNQDGVKVVADKAWFTNRYWLPTDTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPW0</accession>
    <name>I1IPW0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100839128</name>
        <name evidence="8" type="ORF">BRADI_4g29530v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in environmental stress response.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238141.1">
        <property type="nucleotide sequence ID" value="XM_010239839.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPW0"/>
    <dbReference type="FunCoup" id="I1IPW0">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IPW0"/>
    <dbReference type="EnsemblPlants" id="KQJ90117">
        <property type="protein sequence ID" value="KQJ90117"/>
        <property type="gene ID" value="BRADI_4g29530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839128"/>
    <dbReference type="Gramene" id="KQJ90117">
        <property type="protein sequence ID" value="KQJ90117"/>
        <property type="gene ID" value="BRADI_4g29530v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839128"/>
    <dbReference type="eggNOG" id="KOG3183">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061621_1_1_1"/>
    <dbReference type="OMA" id="YKSHECP"/>
    <dbReference type="OrthoDB" id="431929at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677">
        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677:SF20">
        <property type="entry name" value="ZINC FINGER AN1-TYPE CONTAINING 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="7">
        <location>
            <begin position="10"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="7">
        <location>
            <begin position="102"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ90117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="187" mass="20200" checksum="E474E9E64C9B3447" modified="2012-06-13" version="1">MARRGTEAFPELGAHCDREDCNQLDFLPFDCDGCGKVFCAEHRTYRGHGCARAADQGRTVVVCEACGDAIERPAAAGGGREKTDAEILEAHARSRRGCDPARKRKPRCPAPRCKETLTFSNTSECKGCGQKLCLRHRFPADHACARVSPAGAAAAARRKGAGACARDAQKKDGGWTLPPLIRNLKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQ55</accession>
    <name>I1IQ55_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="7">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100838839</name>
        <name evidence="18" type="ORF">BRADI_4g30330v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the CCR4-NOT complex, at least composed of CRR4 and CAF1 proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CAF1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="7"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578122.1">
        <property type="nucleotide sequence ID" value="XM_003578074.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQ55"/>
    <dbReference type="STRING" id="15368.I1IQ55"/>
    <dbReference type="EnsemblPlants" id="KQJ90246">
        <property type="protein sequence ID" value="KQJ90246"/>
        <property type="gene ID" value="BRADI_4g30330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838839"/>
    <dbReference type="Gramene" id="KQJ90246">
        <property type="protein sequence ID" value="KQJ90246"/>
        <property type="gene ID" value="BRADI_4g30330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838839"/>
    <dbReference type="eggNOG" id="KOG0304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027974_0_1_1"/>
    <dbReference type="OMA" id="RTPNCAS"/>
    <dbReference type="OrthoDB" id="1164111at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000027">
        <property type="entry name" value="Putative CCR4-associated factor 1 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039637">
        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10797">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQJ90246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="279" mass="30853" checksum="B67DA927DBED1182" modified="2012-06-13" version="1">MTTPVEDKPDDVEIREVWAENLEAEFAVIREIVDDYPYVAMDTEFPGVVCRPLGTFKSAADFNYATLKANVDMLKLIQLGLTFSDERGGLPALGPGGRPCVWQFNFRGFDPRTDVAAADSIDLLRRSGIDFARHAADGADSRRFAELLMSSGVVMNAEIHWVTFHSGYDFGYLLKLLTGTNLPDTNSGFFDLIRIYFPVIYDIKHLMRFCNSLHGGLNKLAELLDVERVGICHQAGSDSLLTALSFNKLKESYFGGLTEKYAGVLYGLGTEGGETTSVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1IRI1</accession>
    <name>I1IRI1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100837622</name>
        <name evidence="4" type="ORF">BRADI_4g34360v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578372.1">
        <property type="nucleotide sequence ID" value="XM_003578324.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IRI1"/>
    <dbReference type="STRING" id="15368.I1IRI1"/>
    <dbReference type="EnsemblPlants" id="KQJ90888">
        <property type="protein sequence ID" value="KQJ90888"/>
        <property type="gene ID" value="BRADI_4g34360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837622"/>
    <dbReference type="Gramene" id="KQJ90888">
        <property type="protein sequence ID" value="KQJ90888"/>
        <property type="gene ID" value="BRADI_4g34360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837622"/>
    <dbReference type="eggNOG" id="KOG0878">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071479_4_1_1"/>
    <dbReference type="OMA" id="ENCAHEF"/>
    <dbReference type="OrthoDB" id="751288at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ90888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15662" checksum="4C8A1AB1B7BE7B10" modified="2012-06-13" version="1">MAVPLLTRKIVKKRVKQFKRAHSDRYIGLKTSWRRPKGIDSRVRRKFKGCTLMPNIGYGSDKKTRHYLPNKFKKFVVHNVSELELLMMHNRTYCAEIAHNVSTKKRKEIVERAAQLDVVVTNKLARLRSQEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IT04</accession>
    <name>I1IT04_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine-containing phosphotransfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100826811</name>
        <name evidence="10" type="ORF">BRADI_4g38450v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as a two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction through the multistep His-to-Asp phosphorelay. Functions as a positive regulator of the cytokinin signaling pathway. May play a regulatory role in salt and drought tolerance during plant development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578675.1">
        <property type="nucleotide sequence ID" value="XM_003578627.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IT04"/>
    <dbReference type="FunCoup" id="I1IT04">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IT04"/>
    <dbReference type="EnsemblPlants" id="KQJ91580">
        <property type="protein sequence ID" value="KQJ91580"/>
        <property type="gene ID" value="BRADI_4g38450v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826811"/>
    <dbReference type="Gramene" id="KQJ91580">
        <property type="protein sequence ID" value="KQJ91580"/>
        <property type="gene ID" value="BRADI_4g38450v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826811"/>
    <dbReference type="eggNOG" id="KOG4747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111777_3_0_1"/>
    <dbReference type="OMA" id="NMFTTGM"/>
    <dbReference type="OrthoDB" id="1673781at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043424">
        <property type="term" value="F:protein histidine kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080038">
        <property type="term" value="P:positive regulation of cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.160:FF:000001">
        <property type="entry name" value="Histidine-containing phosphotransfer protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045871">
        <property type="entry name" value="AHP1-5/YPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242:SF30">
        <property type="entry name" value="HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242">
        <property type="entry name" value="PHOSPHORELAY INTERMEDIATE PROTEIN YPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4 8" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HPt" evidence="9">
        <location>
            <begin position="38"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="7">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ91580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="145" mass="16094" checksum="062818EAB2BCC4FB" modified="2012-06-13" version="1">MAAAALKGQLNTLLSSMFSEGLVDEQFQQLRMLQEDGAPGFVAEVVTLFCDDADRIIAEIANLLEQPVVDFDKVDGSVHQLKGSSASVGAQKVKLSCMQFRQFYEAKSKDGCLMALSVVRNAFYEMRSNFQTMLQLEQQIQAYDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IT56</accession>
    <name>I1IT56_BRADI</name>
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100833745</name>
        <name evidence="3" type="ORF">BRADI_4g38855v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578698.1">
        <property type="nucleotide sequence ID" value="XM_003578650.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238547.1">
        <property type="nucleotide sequence ID" value="XM_010240245.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IT56"/>
    <dbReference type="STRING" id="15368.I1IT56"/>
    <dbReference type="EnsemblPlants" id="KQJ91639">
        <property type="protein sequence ID" value="KQJ91639"/>
        <property type="gene ID" value="BRADI_4g38855v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833745"/>
    <dbReference type="Gramene" id="KQJ91639">
        <property type="protein sequence ID" value="KQJ91639"/>
        <property type="gene ID" value="BRADI_4g38855v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833745"/>
    <dbReference type="eggNOG" id="KOG1347">
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        <property type="expression patterns" value="baseline and differential"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR34797">
        <property type="entry name" value="ATG8-INTERACTING PROTEIN 2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ91639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="278" mass="29580" checksum="11460AB8AFA04251" modified="2012-06-13" version="1">MADADNWELVSLTASTYAAAPGPISPVLLLDAHKTGELSNPPPPPPPAIFMSQHFNWSEAVEALAQLNGRQEESTPPAVDTNAMVDDDTHLDMLCPENCEREQLDDSLARSDSRSNTLDDGNFSLAGEERQTEIFCSADAHAAAAYSDIRGTSAPHVSAAAAAAAAAIVETASHVPVAVSSSPRIAPRMPREAAQAWWKKTFSFLRNNARESLTFRVIFVAATVVGLAILGQREKLQLQQLRLEFDVHSEKISCGSVGEPLGQMKGGMIVGGSPVAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IUW5</accession>
    <name>I1IUW5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SANTA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100832997</name>
        <name evidence="3" type="ORF">BRADI_4g44060v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577118.1">
        <property type="nucleotide sequence ID" value="XM_003577070.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IUW5"/>
    <dbReference type="STRING" id="15368.I1IUW5"/>
    <dbReference type="EnsemblPlants" id="KQJ92495">
        <property type="protein sequence ID" value="KQJ92495"/>
        <property type="gene ID" value="BRADI_4g44060v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832997"/>
    <dbReference type="Gramene" id="KQJ92495">
        <property type="protein sequence ID" value="KQJ92495"/>
        <property type="gene ID" value="BRADI_4g44060v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832997"/>
    <dbReference type="eggNOG" id="ENOG502S4QI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023651_0_0_1"/>
    <dbReference type="OMA" id="PECANCC"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IUW5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053090">
        <property type="entry name" value="Centromere_KNL-2_homolog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015216">
        <property type="entry name" value="SANTA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35311">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN KNL-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35311:SF10">
        <property type="entry name" value="OS04G0347900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09133">
        <property type="entry name" value="SANTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANTA" evidence="2">
        <location>
            <begin position="40"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="500" mass="54859" checksum="8193A6BADAC1FE86" modified="2012-06-13" version="1">MASPPEPAIPVLSPLSSRLSAAADSSSVSSSAPVEHPCFTLQNWWLVRVEGEERKIAVSGFTQRGDAFTSAPIAKRHESLVLEDEDGVVVRIDGLMSLCRMRRNGFSLQICESFLIGFPSWWESWDSHFESQPTSSSNSQEDSSQIYLKIFQLGNVVQKSVASFIKNPLHDAKIFRRYVADAFTQCSRFDEYSFDNDTSTKGKTVASNDASEGPAAVANEVDNMEIDLIVSSTSQERGHVDISCNASFAPTEKCTSDETYKEAENQNDSMHPDVTEQEAGNHSVNSDLICNRSRDRMPSDLEDGNTNAGNSTDVALCHLATAQPERVNCCSEIPGALQNIQPLSNQRNPVASLKNQSHPKRTEDISLNQKAVPIEDTSTSIRSHVLSSEKTVGPSKKQRSAQDKLLSPARLRGTRNPISYVHHSPHTRGKAQSLSISTPESLEMTRTKSGRVVVPPLDLGCERILYGNNHLVLGVAPVKLHSPPIKGSKPETPARKRRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVY8</accession>
    <name>I1IVY8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glycosyltransferases</fullName>
            <ecNumber evidence="12">2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100841498</name>
        <name evidence="14" type="ORF">BRADI_5g02350v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in the synthesis of glucuronoxylan hemicellulose in secondary cell walls.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-" evidence="12"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580881.1">
        <property type="nucleotide sequence ID" value="XM_003580833.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVY8"/>
    <dbReference type="EnsemblPlants" id="KQJ81696">
        <property type="protein sequence ID" value="KQJ81696"/>
        <property type="gene ID" value="BRADI_5g02350v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841498"/>
    <dbReference type="Gramene" id="KQJ81696">
        <property type="protein sequence ID" value="KQJ81696"/>
        <property type="gene ID" value="BRADI_5g02350v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841498"/>
    <dbReference type="eggNOG" id="KOG1476">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044006_1_1_1"/>
    <dbReference type="OMA" id="HHHPFAS"/>
    <dbReference type="OrthoDB" id="675023at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IVY8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042285">
        <property type="term" value="F:xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010417">
        <property type="term" value="P:glucuronoxylan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000064">
        <property type="entry name" value="Glycosyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF33">
        <property type="entry name" value="GLUCURONOSYLTRANSFERASE OS04G0103100-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ81696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="396" mass="43866" checksum="A6BB9A36B737B121" modified="2012-06-13" version="1">MASIRRPHSPAKQQHLLRHHHPFASSSPPSSPLRHASSAATTSSSPRHHHGGSYPHPFLFFTRRPLPRFAAFFLLGSFLGLLHFLSHLPLLPHLPVPTNASSSSPQFNHLQQQPALDQQQQATTLGLGGGGENNGDKLLIVVTPTRARASQAYYLSRMGQTLRLARPPVLWVVVEAGKPTPEAAHALRRTAVMHRYVGCCDKLAAAANANASIDYRPHQMNAGLEVVENHRLDGIVYFADEEGVYSLQLFDRLRQIRRFGTWPVPVISDGGNGVVLDGPVCKQNQVVGWHTSGEASKLQRFHVAMSGFAFNSTMLWDPKLRSHQAWNSIRHPEMVEQGFQGTAFVEQLVEDESQMEGIPADCSQIMNWHVPFGSESLVYPKGWRVATNLDVIIPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IX61</accession>
    <name>I1IX61_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ACT domain-containing protein ACR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Protein ACT DOMAIN REPEATS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100841900</name>
        <name evidence="4" type="ORF">BRADI_5g08340v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds amino acids.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579632.1">
        <property type="nucleotide sequence ID" value="XM_003579584.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IX61"/>
    <dbReference type="STRING" id="15368.I1IX61"/>
    <dbReference type="EnsemblPlants" id="KQJ82305">
        <property type="protein sequence ID" value="KQJ82305"/>
        <property type="gene ID" value="BRADI_5g08340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841900"/>
    <dbReference type="Gramene" id="KQJ82305">
        <property type="protein sequence ID" value="KQJ82305"/>
        <property type="gene ID" value="BRADI_5g08340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841900"/>
    <dbReference type="eggNOG" id="ENOG502QT1H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031332_3_0_1"/>
    <dbReference type="OMA" id="HAVRCEA"/>
    <dbReference type="OrthoDB" id="2019938at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IX61">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040217">
        <property type="entry name" value="ACR1-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31096:SF55">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN ACR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13740">
        <property type="entry name" value="ACT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="353"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ82305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="474" mass="52056" checksum="BDE0E358949E0F70" modified="2012-06-13" version="1">MVLIDDQDEYAKLVRGMNPPRVEIDNEISKIGTFIQVDSVNTHGTLLALVQVITDLNLVVRKAYFTADGDWFMDVFYVTDRDGEKVTDEATLNYIQTTLESDDCYYTEARDNSADIVPSESEEDSHQYSSIELTGTDRPGLLSEVCAVLSDVRCAVVSADLWTCNTRVAAVVQVADAATGVAISADPARVAEISRRLAHLLRSRSWCHATVAASVAEEPSLVAMHKERRLHQLMAADPESGVIEGDGAYLQPAPGTTPATVVEVTDCAQRGYTFVVVRCRDVPKLLFDTVCTITDAQYVVYHGNVSTEPDGVTAYQEYYVRNKAGLAATEPERLLLKRQLEAAVERRFADGIELEVRSGDRAGLLSDVTRIIRENGLTILRAGVKSQGGEAVDTFYVSDPMGLDYPVEPRTIDTIRAQIGEATLRVKKNPFADADQQQQQHDAAASVVGAIAFILGSVYKFYRPFQSLALVKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1IYV8</accession>
    <name>I1IYV8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Isopropylmalate dehydrogenase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100843832</name>
        <name evidence="14" type="ORF">BRADI_5g13490v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Performs an essential role in the oxidative function of the citric acid cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579972.1">
        <property type="nucleotide sequence ID" value="XM_003579924.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IYV8"/>
    <dbReference type="FunCoup" id="I1IYV8">
        <property type="interactions" value="1017"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IYV8"/>
    <dbReference type="EnsemblPlants" id="KQJ83166">
        <property type="protein sequence ID" value="KQJ83166"/>
        <property type="gene ID" value="BRADI_5g13490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843832"/>
    <dbReference type="Gramene" id="KQJ83166">
        <property type="protein sequence ID" value="KQJ83166"/>
        <property type="gene ID" value="BRADI_5g13490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843832"/>
    <dbReference type="eggNOG" id="KOG0784">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_1"/>
    <dbReference type="OMA" id="PWSCDYY"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IYV8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000009">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF56">
        <property type="entry name" value="NAD-DEPENDENT ISOCITRATE DEHYDROGENASE C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="13">
        <location>
            <begin position="43"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ83166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="371" mass="39914" checksum="CA36B629CDA44FE6" modified="2012-06-13" version="1">MARRSAPLLRRLVSSPPPLPGHGAAARRTVTYMPRPGDGTPRPVTLIPGDGIGPLVTGSVQQVMDAMHAPVYFETYDVHGDMPSVPPAVIDSIRRNKVCLKGGLATPVGGGVSSLNMQLRKELDLYASLVNCANVPGLPTRHKNVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNYRKKVTAVHKANIMKLADGLFLESCREVASKYPGIEYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLVGGTGVMPGGNVGQDHAIFEQGASAGNVGNDNLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKRVVAEGKYRTKDLGGTSTTQEVTDAVIANLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IYW7</accession>
    <name>I1IYW7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100839670</name>
        <name evidence="6" type="ORF">BRADI_5g13537v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240028.1">
        <property type="nucleotide sequence ID" value="XM_010241726.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IYW7"/>
    <dbReference type="EnsemblPlants" id="PNT61305">
        <property type="protein sequence ID" value="PNT61305"/>
        <property type="gene ID" value="BRADI_5g13537v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839670"/>
    <dbReference type="Gramene" id="PNT61305">
        <property type="protein sequence ID" value="PNT61305"/>
        <property type="gene ID" value="BRADI_5g13537v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839670"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_37_8_1"/>
    <dbReference type="OMA" id="KSCSRMC"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000490">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000031">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF458">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="189"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="259"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="290"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="391"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="5">
        <location>
            <begin position="606"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT61305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="698" mass="77527" checksum="8316D5747EF8778F" modified="2012-06-13" version="1">MAATALPAARLPAPGMPSPARQTPRRAALRDVPQLHATLLKSGAMTTSPDSFHSLLEAAALPAPATSSAHLSYAIRLFRLGPHPPRSARSYNILIRSFLRAGHPEDALHLFVEMLDDTAVSPDQHTVANTVKSCSRMCDLSVGRGVQAYAFKRGFMVDQFVLNSLIHMYASCGDVVAAHVLFHTVQVKGVIAWNAMIAGYVKNGDWKEVVEMFKGMLEVRAPFDEVTLLSVATACGRLGDANLGQWIAEYAEEKGMLRSRNLATALVDMYAKCGELDKARRLFDRMHSRDVVAWSAMISGYTQSDRCREALAIFNEMQGTEVNPNDVTMVSVLSACAVLGALETGKWVHSYIRRKDLPLTVILGTALVDFYAKCGCIKDAVKAFESMPVRNTWTWTALIKGMASNGRSREALELFSSMLEANIEPTDVTFIGVLLACSHGCLVEEGRRHFTSMTQDYGICPRIEHYGCMVDLLGRAGLIDEAYQFIRNMPIEPNAVVWRALLSACTVHKNVEIGEEALKQIVPLDPCHSGNYILLSNTYASVGQWKNAAMVRKEMKEKGVEKIPGCSLIELEGTIFEFFAEDSEHPQLTEIYEKVHEMIENIKMVGYIPNTADARLDVDEYEKQVSVSHHSEKLAIAFGLMKSRPGATIRLSKNLRVCIDCHSATKLISKVYNREIIVRDRNRFHHFKDGLCSCNDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0N0</accession>
    <name>I1J0N0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100834167</name>
        <name evidence="5" type="ORF">BRADI_5g18520v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240268.1">
        <property type="nucleotide sequence ID" value="XM_010241966.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0N0"/>
    <dbReference type="STRING" id="15368.I1J0N0"/>
    <dbReference type="EnsemblPlants" id="KQJ84074">
        <property type="protein sequence ID" value="KQJ84074"/>
        <property type="gene ID" value="BRADI_5g18520v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ84075">
        <property type="protein sequence ID" value="KQJ84075"/>
        <property type="gene ID" value="BRADI_5g18520v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834167"/>
    <dbReference type="Gramene" id="KQJ84074">
        <property type="protein sequence ID" value="KQJ84074"/>
        <property type="gene ID" value="BRADI_5g18520v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ84075">
        <property type="protein sequence ID" value="KQJ84075"/>
        <property type="gene ID" value="BRADI_5g18520v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834167"/>
    <dbReference type="eggNOG" id="KOG0389">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_16_6_1"/>
    <dbReference type="OMA" id="STRNCES"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J0N0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140750">
        <property type="term" value="F:nucleosome array spacer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071494">
        <property type="term" value="P:cellular response to UV-C"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000729">
        <property type="term" value="P:DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17919">
        <property type="entry name" value="DEXHc_Snf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000038">
        <property type="entry name" value="Protein CHROMATIN REMODELING 19 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="339"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="705"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ84074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="876" mass="98094" checksum="82B8B0B60847EF4B" modified="2012-06-13" version="1">MRRAFEEISDEEWSNHTFKPSRVLKRPHRTSQPAAPPPPPIESFRYDRKASSAVGTSKVTVVLSDDDDDFELGGNVRSRRAGKSSGVLKPPQHSNPPRRGPVSSVPFRHNPQPSKAAITAAFSHDDDFVISDDDFDLPVWRTSRRGRSTGRRLATATVDLSEGEEELDLAYEDSDLADDDSDDLADDNSDHPDPRPSRPRRTTGRRFVIEDDDDSDGSAAVGVMEVGDGEEDDEINLSELEKEDDDDGDYNGERSVKVEEVDGDVVGKALRRCSRISADLRQELYGSSTRNCESYAETDASTCQIVTQEDVDAACTCEELDFEPVLKPYQLVGVNFLVLLHRKKIGGAILADEMGLGKTVQAVTYLNLLQHLYNDPGPHLIVCPASVLENWERELKKWCPSFSVIMFHGAGRAAYSKELSSLGKAGCPPPFNVLLTSYSLFERKSAQQKDDRKALKRWKWSCVLMDEAHVLKDKGSFRWKNLMAVAQHARQRLMLTGTPLQNDLHELWSLLEFMMPDIFATGDVDLKKLLNAEDHELILHIKSILGPFILRRLKSDVMQQLVPKTQHVNFVSMGSEQYKAYKDATEDYRAVCQARSAKSSVQLSNNLSGLIPKRQISNYFMQLRKIANHPLLIRRIYSDKDVERIARLMYPKGAFGFECPLDRAIQELKNSNDFAIHQLLISYGDTGTKGALKDEHVLGSAKCQALAELLPSLANDGHRVLIFSQWTTMLDILEWTLEVIGVTYRRLDGGTPVIERQTIVDTFNNDRSIVACLLSTRAGGQGLNLIGADTVIIHDMDFNPQMDRQAEDRCHRIGQQKPVTIYRLVTKESVDENIYAIARRKLVLDAAILQSGADVDDETDVPEKTMGEILASLLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EL13</accession>
    <name>A0A0Q3EL13_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g16687v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EL13"/>
    <dbReference type="EnsemblPlants" id="KQJ88271">
        <property type="protein sequence ID" value="KQJ88271"/>
        <property type="gene ID" value="BRADI_4g16687v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88271">
        <property type="protein sequence ID" value="KQJ88271"/>
        <property type="gene ID" value="BRADI_4g16687v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EL13"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033238934" evidence="2">
        <location>
            <begin position="26"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ88271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="178" mass="19642" checksum="989B34A783EA3BA3" modified="2016-01-20" version="1">MQILPPFLFSSRILLLLLLLHCTLLPPPPTPREEHQDEMKSKRKIEMFDEKSKRACLPSVEDDEEEDTEEEKRVGGGRPVRHSEHCDPRGSHRDEPDGLGEGARGLIHSSMHDLSDDEPVEDGVRGVGHDTSMGDMHERGHGSYGLSSKLSIGREEEGDQHSEGRACGLDDGVCGLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EXQ5</accession>
    <name>A0A0Q3EXQ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Beta-galactosidase</fullName>
            <ecNumber evidence="4 11">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100841567</name>
        <name evidence="15" type="ORF">BRADI_4g36517v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="4 11"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576777.1">
        <property type="nucleotide sequence ID" value="XM_003576729.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EXQ5"/>
    <dbReference type="FunCoup" id="A0A0Q3EXQ5">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ91244">
        <property type="protein sequence ID" value="KQJ91244"/>
        <property type="gene ID" value="BRADI_4g36517v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841567"/>
    <dbReference type="Gramene" id="KQJ91244">
        <property type="protein sequence ID" value="KQJ91244"/>
        <property type="gene ID" value="BRADI_4g36517v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841567"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EXQ5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019388">
        <property type="term" value="P:galactose catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009827">
        <property type="term" value="P:plant-type cell wall modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22842">
        <property type="entry name" value="Gal_Rha_Lectin_BGal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000050">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000098">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041392">
        <property type="entry name" value="GHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17834">
        <property type="entry name" value="GHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129075" description="Beta-galactosidase" evidence="13">
        <location>
            <begin position="23"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="14">
        <location>
            <begin position="749"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQJ91244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="835" mass="94061" checksum="9D5921F0FEDF1C47" modified="2016-01-20" version="1">MATAVLLLAAAVSALLAGEAEAAWSLTKKGTVVSYDERSLMIDGKRDLFFSGAIHYPRSPPEMWPKLLDRAKDGGLNTIETYVFWNAHEPEPGKYNFEGRCDLIKFLKLIQDNDMYAVIRIGPFIQAEWNHGGLPYWLREIPHIIFRANNEPYKKEMEKFVRFIVQKLKDADMFASQGGPIILAQIENEYGNIKKDHITDGDKYLEWAAEMALSTNIGIPWIMCKQTTAPGVVIPTCNGRHCGDTWTLRDKNKPRLWTENWTAQFRAFGDQAAVRSAEDIAYSVLRFFAKGGTLVNYYMYYGGTNFGRTGASYVLTGYYDEAPIDEYGLNKEPKFGHLRDLHKLIKSYHKAFLVGKQSFELLGHGYEAHNYELPEENLCLAFISNNNTGEDGTVMFRGKKYYIPSRSVSILADCNHVVYNTKRVFVQHSERSFHTADESTKNNVWEMYSEPIPRYKVTSVRTKEPLEQYNLTKDKSDYLWYTTSFRLEADDLPFRRDIRPVVQVKSSAHAMMGFVNDAFAGSGRGSKKDKGFLFEKPIDLRIGINHLALLSSSMGMKDSGGELVEVKGGIQDCMIQGLNTGTLDLQGNGWGHKINLDGEDKEIYTEKGMGTVKWKPAENGHAVTWYRRYFDEPDGDDPVVLDMSSMSKGMIFVNGEGVGRYWTSYKTIAGLPSQSLYHIPRPFLKSKKNLLVVFEEEIGKPEGILIQTVRRDDICFLMSEHNPAQVKTWDADGGQIKLIAEDHSSRGILTCPHKKTIEEVVFASFGNPEGACGNFTAGTCHTPNAKEFVAKECLGKKSCVLPLIHTLYGADINCPTTTATLAVQVRCHRKGKKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F2Q8</accession>
    <name>A0A0Q3F2Q8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC112271515</name>
        <name evidence="2" type="ORF">BRADI_3g06251v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316499.1">
        <property type="nucleotide sequence ID" value="XM_024460731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F2Q8"/>
    <dbReference type="EnsemblPlants" id="KQJ93710">
        <property type="protein sequence ID" value="KQJ93710"/>
        <property type="gene ID" value="BRADI_3g06251v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112271515"/>
    <dbReference type="Gramene" id="KQJ93710">
        <property type="protein sequence ID" value="KQJ93710"/>
        <property type="gene ID" value="BRADI_3g06251v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="611323at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF84">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
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        </location>
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            <begin position="47"/>
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    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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        </location>
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        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK03320.1"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="126" mass="14179" checksum="D1D6F6271CECE1FD" modified="2016-01-20" version="1" fragment="single">KHRIISPKCLCSSSNHLQKYLLPHGALPFFSPRAILPRPARRHRRCLLNPPLQSTTTQTPSRAPTSTTPPTPSSNASDDHAAAADPRQQEDRVLHPHPHRPPRRRPPHERHLLWIGGSNIYGRSLS</sequence>
</entry>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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            <strain evidence="10 11">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Schmutz J."/>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    <dbReference type="KEGG" id="bdi:100836853"/>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
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            <taxon>BOP clade</taxon>
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CemA family.</text>
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    <dbReference type="EMBL" id="CM000881">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblPlants" id="KQK04344">
        <property type="protein sequence ID" value="KQK04344"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="14338" checksum="BB93CB44B17E6E40" modified="2018-03-28" version="2" fragment="single">MKKKKALPSLLYLVFIVLLPWGASFSVNKCLELWIKNWWNTRQSETLLTDIQEKRILERFIELEELSLLDEMIKEKPKTHVQKPPIGIHKEIIQWVKIDNEDHLHIILHFSTNIICLAILS</sequence>
</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="5">cv. Bd21</strain>
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        </source>
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</entry>
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    <accession>A0A0Q3GDF1</accession>
    <name>A0A0Q3GDF1_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC104582838</name>
        <name evidence="2" type="ORF">BRADI_2g46665v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </dbReference>
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        <property type="gene ID" value="BRADI_2g46665v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104582838"/>
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        <property type="gene ID" value="BRADI_2g46665v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104582838"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="97"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK09207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GII2</accession>
    <name>A0A0Q3GII2_BRADI</name>
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        <recommendedName>
            <fullName evidence="8">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100824800</name>
        <name evidence="5" type="ORF">BRADI_2g57107v3</name>
    </gene>
    <organism evidence="5">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003567291.4"/>
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    <dbReference type="STRING" id="15368.A0A0Q3GII2"/>
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        <property type="protein sequence ID" value="KQK10927"/>
        <property type="gene ID" value="BRADI_2g57107v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824800"/>
    <dbReference type="Gramene" id="KQK10927">
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        <property type="gene ID" value="BRADI_2g57107v3"/>
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    <dbReference type="KEGG" id="bdi:100824800"/>
    <dbReference type="OrthoDB" id="1870062at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GII2">
        <property type="expression patterns" value="baseline and differential"/>
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        <property type="evidence" value="ECO:0007669"/>
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    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="At5g08430-like"/>
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    <dbReference type="PANTHER" id="PTHR46851">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46851:SF21">
        <property type="entry name" value="OS01G0884500 PROTEIN"/>
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    </dbReference>
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    <dbReference type="Pfam" id="PF22908">
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    </dbReference>
    <dbReference type="Pfam" id="PF02201">
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    </dbReference>
    <dbReference type="SMART" id="SM00444">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00719">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF159042">
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    <dbReference type="SUPFAM" id="SSF47592">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="4">
        <location>
            <begin position="249"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Plus3" evidence="3">
        <location>
            <begin position="379"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="770"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="706"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="710"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK10927.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK10927"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="827" mass="93891" checksum="E9103A8A7984ACDD" modified="2016-01-20" version="1">MAMDGIKPRRWGKRADDYMAEFMCFVCMDAGHVRVCDVENCLKAFHPSCVGKKDDLFMSDEEFICVCHTCVNCKRSSVYRCLCCPRSACEECLGEIEFVQVKQIRELSASHGVILGDFKSAQMKQGKGFCSTCLNLTLVLEKYADADYEKARADFGRTEYYESGFVGYWSFIKDQEELTLLDLRIARHLLKRSQSSKEGRDSEKSPEQHRKTDGSSSGDNDNAGETFRPDKMDIPNEVQASLKRRKAKKKTYVGWASKELTDFLSCIGKDTTKPIEHFKVTEVVKEYIRQRNLFQDKKKKSVVCDDNLHSLFSKRKVKYNLIHGLLDTHFAANAISESEDETDGSDYDDGSTVQKKLRNCLEPTILKRVPEVNKRCLASLNQKNLNLIYLRRTLVTKLLGQPDTFEQKVLGCLVRVKNDLKSYSYQMSKKYYQIGFVTGIKRSSEEYKIKDTCTDILFCVSSMWDDVKITMLSEEDFEEDECHDILLTKKEPLKRPTVAELEEKVASVHADIVNHWMDRELLRLEKQIERAFDKGWRCEAHDLLCKQKLLRAPAERQRRLEEIPEVIPDPEEENKEAETESAASNFSQGNRGIKRKIAACMNFAEEKIKDATTQVPVSLETIIEETAEGATVQPCDSLEVVPEESREGVTQQVTDPLAVGIQESPEGASVQIDSASEVASQADGKALCNADTPRSGLHTLVLPGQVTADGEDKNGTRHHNEIIDLDGDDDELHAEQREPEAAQRGSPEALTASGPLFTVTPSAPDGPDSVKMWYYMDPQGQQQGPFSMFQLRFWHRLDWFDKDFRVWHIGQKPKRAILVADAVQMIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GRA1</accession>
    <name>A0A0Q3GRA1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Caleosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g15418v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the caleosin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GRA1"/>
    <dbReference type="STRING" id="15368.A0A0Q3GRA1"/>
    <dbReference type="EnsemblPlants" id="KQJ83522">
        <property type="protein sequence ID" value="KQJ83522"/>
        <property type="gene ID" value="BRADI_5g15418v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61454">
        <property type="protein sequence ID" value="PNT61454"/>
        <property type="gene ID" value="BRADI_5g15418v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83522">
        <property type="protein sequence ID" value="KQJ83522"/>
        <property type="gene ID" value="BRADI_5g15418v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61454">
        <property type="protein sequence ID" value="PNT61454"/>
        <property type="gene ID" value="BRADI_5g15418v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GRA1"/>
    <dbReference type="OrthoDB" id="640742at2759"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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    <dbReference type="PANTHER" id="PTHR31495:SF20">
        <property type="entry name" value="CALEOSIN-RELATED FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31495">
        <property type="entry name" value="PEROXYGENASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05042">
        <property type="entry name" value="Caleosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ83522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20513" checksum="41823719C72FECC2" modified="2016-01-20" version="1">MAADGVLPEAMSSVAEEAPVTADRPVRADLETSLPKPYLARALVAPDVNHPEGTGEGHVHRQRSVLQQHVSFFDLDGDGVVYPWETYGGLRALGFNVIVSFVLAIAINVGLSIPTLPSWIPSPLFPVYIKNIHRAKHGSDTFTYDTEGRFMPVNFESIFSKNAHTEPDKLTLGEIWRMTEGQRLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H075</accession>
    <name>A0A0Q3H075_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g06916v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86650.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H075"/>
    <dbReference type="EnsemblPlants" id="KQJ86650">
        <property type="protein sequence ID" value="KQJ86650"/>
        <property type="gene ID" value="BRADI_4g06916v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ86650">
        <property type="protein sequence ID" value="KQJ86650"/>
        <property type="gene ID" value="BRADI_4g06916v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H075"/>
    <dbReference type="OrthoDB" id="669288at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H075">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835:SF85">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ86650.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25435" checksum="C4A44AF590F4A6F9" modified="2018-03-28" version="2">MENEVQEDVEDANNVEAKKQTLFNSASPMKVIKVCKLLDRKQRKKIMKVGFGSFLDIKCSKLFPELCKYLMDHFDPDTSALEFPEDERGIIPIIVDTVFEVLGIPKGALPVIYQYDSVSVKYVLDLLQVEDGRQPKISDIETRLTEMGTAEDPFLLLWMLYVVCFLAPTTGVRVSPNCYPSLVNVSQINKLDWCRFTINILIQTAKAKNKNCFKACMPLLMVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H5Y8</accession>
    <name>A0A0Q3H5Y8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_1g41850v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H5Y8"/>
    <dbReference type="STRING" id="15368.A0A0Q3H5Y8"/>
    <dbReference type="EnsemblPlants" id="KQK18344">
        <property type="protein sequence ID" value="KQK18344"/>
        <property type="gene ID" value="BRADI_1g41850v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18344">
        <property type="protein sequence ID" value="KQK18344"/>
        <property type="gene ID" value="BRADI_1g41850v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H5Y8"/>
    <dbReference type="OrthoDB" id="275301at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H5Y8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052751">
        <property type="entry name" value="Plant_MAPKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48011:SF24">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="288"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="352"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK18344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="474" mass="50373" checksum="DE6DA460C161368A" modified="2016-01-20" version="1">MIVGEWTRGPAIGRGSSAVVSLAVDRVTGVVFAVKSVGAGRAAELRREQSILRGLSSPYVVRCLGYVADGSGEMLMEYAAGGSLADEIRRCGGRCPEALIRCRARDVLRGLAHAHGAGVAHCDVKARNVLVGSHGRAMLADFGCARRIVASSREPQLMGMGGTPMFMAPEAARGEERGAAADVWAVGCTVIEMATGGAPWRSRFADPVAALHHVAFSGEAPELPPWLSEAGKEFLGRCLRQDPRERWTAEQLLEHPWFLQADADAVSCSSSFLPGITGKETTFVSPKSVLDQVPWDDDDTRTGADNDPTDRVRALSGGGAPDWTWDGSWITVHSGDNDDHHDAMWADQPEVDADTTADSDPLVGGSAGRPSVGISNGHASHGNGDSYDDTSSSCDGRCAVEVNLLVPFIEPRASPTGINPFRWLDRRLTPTSSCSVFLCRKLTPPSAPPLHHLAHKPDADELPAQSSSAVLLCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H749</accession>
    <name>A0A0Q3H749_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g44611v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H749"/>
    <dbReference type="EnsemblPlants" id="KQK18775">
        <property type="protein sequence ID" value="KQK18775"/>
        <property type="gene ID" value="BRADI_1g44611v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18775">
        <property type="protein sequence ID" value="KQK18775"/>
        <property type="gene ID" value="BRADI_1g44611v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H749"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H749">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK18775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="127" mass="14145" checksum="1102FA9222A1ADDE" modified="2016-01-20" version="1">MPVDDVRRGVRSRLAQVGCGRCGRAMARRSTAVDSSPRRTDVPLYRCANMPGQQQNFYSSDGYSDLSSSGDNFAKGMHQSIPILAQSEEKRTMRFLVCQASLPTRELTSQITIFCSSHCSRQCQIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HA70</accession>
    <name>A0A0Q3HA70_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GEX2 N-terminal Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC104585100</name>
        <name evidence="6" type="ORF">BRADI_4g30665v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311009.1">
        <property type="nucleotide sequence ID" value="XM_024455241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HA70"/>
    <dbReference type="FunCoup" id="A0A0Q3HA70">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3HA70"/>
    <dbReference type="EnsemblPlants" id="KQJ90298">
        <property type="protein sequence ID" value="KQJ90298"/>
        <property type="gene ID" value="BRADI_4g30665v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104585100"/>
    <dbReference type="Gramene" id="KQJ90298">
        <property type="protein sequence ID" value="KQJ90298"/>
        <property type="gene ID" value="BRADI_4g30665v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5334309at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HA70">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048235">
        <property type="term" value="P:pollen sperm cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Big_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23616">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
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        <location>
            <begin position="1054"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="domain" description="GEX2 N-terminal Ig-like" evidence="5">
        <location>
            <begin position="82"/>
            <end position="181"/>
        </location>
    </feature>
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            <begin position="190"/>
            <end position="296"/>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HFD6</accession>
    <name>A0A0Q3HFD6_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">BRADI_4g09225v3</name>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
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    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ87059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="105" mass="11853" checksum="144AF08AF1355DBB" modified="2016-01-20" version="1">MTSCWQARPCCLSPAVRVLRERQKGRTSTTVRERGVSGRALGRERRAGRRVNLGKKRREEEDGVVADAWDSYISGPARCHVSNPCFGKHVSELIDRNRRAPGCQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HKQ3</accession>
    <name>A0A0Q3HKQ3_BRADI</name>
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    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
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        <name type="synonym">Trachynia distachya</name>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </source>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK23285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="54" mass="6127" checksum="167FD6FA43065256" modified="2016-01-20" version="1">MHLCFYIHFRMGMGRFLELPYATTINAVMITAMVSVKTGVIKRIKVFATNTMDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HL92</accession>
    <name>A0A0Q3HL92_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">BRADI_1g75065v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HL92"/>
    <dbReference type="STRING" id="15368.A0A0Q3HL92"/>
    <dbReference type="EnsemblPlants" id="KQK23631">
        <property type="protein sequence ID" value="KQK23631"/>
        <property type="gene ID" value="BRADI_1g75065v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK23631">
        <property type="protein sequence ID" value="KQK23631"/>
        <property type="gene ID" value="BRADI_1g75065v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HL92"/>
    <dbReference type="OrthoDB" id="684782at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35161">
        <property type="entry name" value="OS02G0303100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35161:SF4">
        <property type="entry name" value="OS02G0303100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK23631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="169" mass="19859" checksum="2ADFE151B86DA272" modified="2016-01-20" version="1">MESPLEHLTRGNVLKMANELIGEYMPHSQQDKFYVQEHTKLLVRSMAKDLKRHHDQGHCVSSFNHWNIKISASGRARIIGVTSVDKNENSIRQNYKDLHNIVLNTIFRDFSLQHQLPSDWRSLVLLMKMHPIGNEYLICNYVALMPIENRIFFFHPMNMSGLYWPKPTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HMR6</accession>
    <name>A0A0Q3HMR6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g25936v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HMR6"/>
    <dbReference type="EnsemblPlants" id="KQJ89475">
        <property type="protein sequence ID" value="KQJ89475"/>
        <property type="gene ID" value="BRADI_4g25936v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89475">
        <property type="protein sequence ID" value="KQJ89475"/>
        <property type="gene ID" value="BRADI_4g25936v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HMR6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ89475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="60" mass="7027" checksum="DD90BEBED6210454" modified="2016-01-20" version="1">MFMRGRINQPLYHLDRFLSVCINLRTSKNRFFNLVGVLLGRLPAEYLLTSGDVLSHWSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HWA1</accession>
    <name>A0A0Q3HWA1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100839746</name>
        <name evidence="8" type="ORF">BRADI_4g44370v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577134.3">
        <property type="nucleotide sequence ID" value="XM_003577086.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HWA1"/>
    <dbReference type="STRING" id="15368.A0A0Q3HWA1"/>
    <dbReference type="EnsemblPlants" id="KQJ92547">
        <property type="protein sequence ID" value="KQJ92547"/>
        <property type="gene ID" value="BRADI_4g44370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839746"/>
    <dbReference type="Gramene" id="KQJ92547">
        <property type="protein sequence ID" value="KQJ92547"/>
        <property type="gene ID" value="BRADI_4g44370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839746"/>
    <dbReference type="OrthoDB" id="2021064at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31282">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="143"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ92547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="304" mass="33631" checksum="66575111ECC1DFFA" modified="2016-01-20" version="1">MKVEAKNSSRHPSPRGRHGSSPSQSQVEALREMARGQSLVAQLRAVVLPALVDDERAGVVAQMFQSVLDCSAKAMAALKLCQIENQARADADGVLMADDKKRSVKKVVVVSDDGDNAKPHRQRKRRRLADECVTLETPVPHYDGHQWRKYGQKVINNAKHPRSYYRCTYGQEQGCKATKTVQQKDDNGAGAVYEDDQVMFAVVYYGQHTCKPPSSSDTDGTVVDSDSRCSSNISVTCTSVAVIDHHRQSSLESSLLDMAEEDLATEEYDQLFDVAAYEPLDSDPAWEMDDAHGHGHGILKFGHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IET4</accession>
    <name>A0A0Q3IET4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Photosystem I iron-sulfur center</fullName>
            <ecNumber evidence="3">1.97.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">9 kDa polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">PSI-C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Photosystem I subunit VII</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">PsaC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">BRADI_2g13635v3</name>
    </gene>
    <organism evidence="27">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">Bd21</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Bd21</strain>
        </source>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="28">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Apoprotein for the two 4Fe-4S centers FA and FB of photosystem I (PSI); essential for photochemical activity. FB is the terminal electron acceptor of PSI, donating electrons to ferredoxin. The C-terminus interacts with PsaA/B/D and helps assemble the protein into the PSI complex. Required for binding of PsaD and PsaE to PSI. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>reduced [plastocyanin] + hnu + oxidized [2Fe-2S]-[ferredoxin] = oxidized [plastocyanin] + reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:30407"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10039"/>
            <dbReference type="Rhea" id="RHEA-COMP:10040"/>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
            <dbReference type="ChEBI" id="CHEBI:30212"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="EC" id="1.97.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="25">The eukaryotic PSI reaction center is composed of at least 11 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="24">Plastid thylakoid membrane</location>
            <topology evidence="24">Peripheral membrane protein</topology>
            <orientation evidence="24">Stromal side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.97.1.12" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IET4"/>
    <dbReference type="STRING" id="15368.A0A0Q3IET4"/>
    <dbReference type="EnsemblPlants" id="KQK04459">
        <property type="protein sequence ID" value="KQK04459"/>
        <property type="gene ID" value="BRADI_2g13635v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04459">
        <property type="protein sequence ID" value="KQK04459"/>
        <property type="gene ID" value="BRADI_2g13635v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IET4"/>
    <dbReference type="OrthoDB" id="1865383at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IET4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009522">
        <property type="term" value="C:photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055035">
        <property type="term" value="C:plastid thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:photosynthetic electron transport in photosystem I"/>
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        <property type="entry name" value="Photosystem I iron-sulfur center"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960:SF79">
        <property type="entry name" value="PHOTOSYSTEM I IRON-SULFUR CENTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="4FE4S_FER_2"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="12" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="17" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="26">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="26">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022836"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030218"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032541"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00033423"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00063727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KQK04459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="81" mass="8869" checksum="D10663DC8FD59E09" modified="2016-01-20" version="1">MSHSVKIYDTCIGCTQCVRACPTDVLETIPWDGCKAKQIASAPRTEDCVGCKRCESACPTDFLSVRVYLGPETTRSMALSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IN15</accession>
    <name>A0A0Q3IN15_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g12800v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IN15"/>
    <dbReference type="EnsemblPlants" id="KQJ87656">
        <property type="protein sequence ID" value="KQJ87656"/>
        <property type="gene ID" value="BRADI_4g12800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87656">
        <property type="protein sequence ID" value="KQJ87656"/>
        <property type="gene ID" value="BRADI_4g12800v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IN15"/>
    <dbReference type="OrthoDB" id="692641at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IN15">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
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        <property type="entry name" value="HARPIN-INDUCED PROTEIN 1 CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ87656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="22863" checksum="5642F302EEC75ABF" modified="2016-01-20" version="1">MSEHKPTKDKLKPWRVRLGIVLAVAVVLAATVLLALRYAVIPEVKAAIDDARLDRFALATTNDSSSLGFNVSIALAVRNTNRAMSVKYTEPVVANFVFHDRRLGTAAVAGEGHEHPPRRREVHLLRLGGEVPSGVIGDAAAEEFKKQNASGAFDVELRFDVEISLGIGNTRGMSFSCPLRLQLAPPGPTVVVFRRVYCMHEEQEKNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ING5</accession>
    <name>A0A0Q3ING5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g57873v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ING5"/>
    <dbReference type="EnsemblPlants" id="KQK01732">
        <property type="protein sequence ID" value="KQK01732"/>
        <property type="gene ID" value="BRADI_3g57873v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01732">
        <property type="protein sequence ID" value="KQK01732"/>
        <property type="gene ID" value="BRADI_3g57873v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3ING5"/>
    <dbReference type="OrthoDB" id="676037at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK01732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="103" mass="11876" checksum="D529E5505CE3AC2D" modified="2016-01-20" version="1">MTDIHTWFESFPRKCRQIIIIGSAALCWSIWKSRNAICFQNKYPGDPANLVFSLCYWIDAWSILQKPDTKAQLLRASGILKKVVVEILNKSCGWSSWRRLEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IRI2</accession>
    <name>A0A0Q3IRI2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_2g04723v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02972.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IRI2"/>
    <dbReference type="EnsemblPlants" id="KQK02972">
        <property type="protein sequence ID" value="KQK02972"/>
        <property type="gene ID" value="BRADI_2g04723v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02972">
        <property type="protein sequence ID" value="KQK02972"/>
        <property type="gene ID" value="BRADI_2g04723v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IRI2"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001203">
        <property type="entry name" value="Putative pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF492">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="178"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="209"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="279"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK02972.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="41245" checksum="768709DEA21FBAD5" modified="2018-03-28" version="2">MLLRVYVGMLARGATPDAYTFQPLLKAVTAAAERGAVGDAVHAHVVKFGMELNAHVASSLVLMYAARRDGAVARTLLDAWPARGGDTPVVWNALISGHRRSRQFRLTGVVPTPVTYITVLSACGKDKYIWLGMQVHKCVVASGVLPDLKVGNALIDMYAECSEMDAAWELFEGMEVRNTVSWTSVISGFLRLGQVDQARTLFDCMPERDTVSWTAMIDGYVQAGQFREALEMFREMQFSKVRADEFTMVSVVTACAQLGALETGEWARIYMSRHGIKMDTFVGNALIDMYSKCGSIQQALDVFKEMYIRDKFTWTAVILGLAVNCHGEEAINMFYWMLRVFEAPDEVTFMVSSLHALMLAWSTKDETSFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J640</accession>
    <name>A0A0Q3J640_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubby C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100833083</name>
        <name evidence="4" type="ORF">BRADI_3g05930v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571017.1">
        <property type="nucleotide sequence ID" value="XM_003570969.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J640"/>
    <dbReference type="SMR" id="A0A0Q3J640"/>
    <dbReference type="FunCoup" id="A0A0Q3J640">
        <property type="interactions" value="1017"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3J640"/>
    <dbReference type="EnsemblPlants" id="KQJ93640">
        <property type="protein sequence ID" value="KQJ93640"/>
        <property type="gene ID" value="BRADI_3g05930v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833083"/>
    <dbReference type="Gramene" id="KQJ93640">
        <property type="protein sequence ID" value="KQJ93640"/>
        <property type="gene ID" value="BRADI_3g05930v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833083"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009620">
        <property type="term" value="P:response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF131">
        <property type="entry name" value="TUBBY-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="3">
        <location>
            <begin position="140"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ93640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="394" mass="42804" checksum="E04E2B55B7680C93" modified="2016-01-20" version="1">MAAATTTPAKREPLRPLSCNAGAAAVAVVRPRGAATASAEKENQGAPNLGPDKEEKVKVAPPPPLKPSSLQARMEGEAPLAAAAPAAVVPVFMGPRGRELLPPATPAAAAAASYEAWDLSDSEAAPAASWATLPNRALLCRPLPLDVGRCTCVVVREKAAGPRGVALYSLYTNEGQGRQDRKLAVARHRRRRGRSEFIVAQNQDGVFCSSDKNFLGTVGANLVGSKYQIWGQGNRIDELNSQSKRLLGVVAFAPTITTLTGSFRSMRAWIPKSQSMQLRTNSSAQIQHIGGLPKDWEEKASRADKLCSRSPFYNNMTKRYELDFRERVGRMGYKVQTSVKNFQMTLEENGRQTILQLGRVGKSKYIMDFRYPLTGYQAFCICLASMDSKLCCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JB60</accession>
    <name>A0A0Q3JB60_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g17651v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JB60"/>
    <dbReference type="EnsemblPlants" id="KQJ95528">
        <property type="protein sequence ID" value="KQJ95528"/>
        <property type="gene ID" value="BRADI_3g17651v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95528">
        <property type="protein sequence ID" value="KQJ95528"/>
        <property type="gene ID" value="BRADI_3g17651v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JB60"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ95528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="209" mass="20414" checksum="7E8ACD120193EF27" modified="2016-01-20" version="1">MAFRGGGRGGGEYQGGDRFYDRDRRGYGGGGSRGSFNDGYGDNEGYGDGREDFGRADFGGYNGDRFSGGRDGDGFCGNFHPGRWFAGPGGPPRDGGGNRRRYEFRTGHGSAGHGRGLPPARGRGSGAGRGGGGGKGAAATSTSAGVTAVAETGATNASASATGVVSGEGQAAAVASAPLAAAVPVVSSAGLVGAAMPLGAGSSSFSHCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JI54</accession>
    <name>A0A0Q3JI54_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g33480v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JI54"/>
    <dbReference type="STRING" id="15368.A0A0Q3JI54"/>
    <dbReference type="EnsemblPlants" id="KQJ97816">
        <property type="protein sequence ID" value="KQJ97816"/>
        <property type="gene ID" value="BRADI_3g33480v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97816">
        <property type="protein sequence ID" value="KQJ97816"/>
        <property type="gene ID" value="BRADI_3g33480v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JI54"/>
    <dbReference type="OrthoDB" id="777875at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JI54">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043424">
        <property type="entry name" value="BLT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31071">
        <property type="entry name" value="GB|AAF24581.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31071:SF39">
        <property type="entry name" value="PROTEIN BRANCHLESS TRICHOME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ97816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="320" mass="34277" checksum="F2A68C82BEF6585E" modified="2016-01-20" version="1">MAEPRRSDAGREDEMGEGRNQQTGSAHKGKARSEAAGAAKPLFPASKCRIIGSRARKDGVELSRSGGRTSMIVASFDDGDGMERQTMGLGGGYGGVSVSELVARVEELGAELEFERRMRRKVETVNEALAAELAEERRRGEAERARAAGLARAREEEVDEERRMLRVAELWREERVRMKLADARAAVEDALREVADAGHRATIATADRCSCCRSSGGGAGSPIGKPTGTPASGQQSPASGQHGQSHRREATTGGGGGENPHIRRGIKGSVEFPRAVRVRPRGGERADVVSNLECQRAQLRVLMRHRSPADAGAPPDNLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JJU8</accession>
    <name>A0A0Q3JJU8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_3g37755v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JJU8"/>
    <dbReference type="FunCoup" id="A0A0Q3JJU8">
        <property type="interactions" value="489"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ98576">
        <property type="protein sequence ID" value="KQJ98576"/>
        <property type="gene ID" value="BRADI_3g37755v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ98576">
        <property type="protein sequence ID" value="KQJ98576"/>
        <property type="gene ID" value="BRADI_3g37755v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JJU8"/>
    <dbReference type="OrthoDB" id="642536at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165:SF62">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="2">
        <location>
            <begin position="57"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ98576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="332" mass="35896" checksum="C9CD72B974C5DEAA" modified="2016-01-20" version="1">MDTYMALRGVCRSWRNATAGPAVDGTMVDGATDPLFMLSKWAMVTQSLRADAGVTLVNLSTGRFLSKRIPLLLRYFFLGATGGGLLVLGEREPPHLMRVFNPLTGAMARFNLPVVRSEVAAVAVTPSPLMVFISVRHEDAINMTPFDGNVHLTNWGSIISTFQRVVVDGAGTTRSAQTIGIATTIPPLSSAGTSLFYLVESAGELLLVTKKWSFVPHLVYKVDMVKKAFEPVRSIGNRALFVSSGRCFSVDADKFPGVEPDRIYSVELSLTGAPGFDSYKVTKVHVAGGGPAETIEDENVLLRRGCFQPARLVQVFAEYCKFVGYPGLGLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JXL1</accession>
    <name>A0A0Q3JXL1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(m1A58)-methyltransferase subunit TRM6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_2g06310v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRM6/GCD10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JXL1"/>
    <dbReference type="STRING" id="15368.A0A0Q3JXL1"/>
    <dbReference type="EnsemblPlants" id="KQK03209">
        <property type="protein sequence ID" value="KQK03209"/>
        <property type="gene ID" value="BRADI_2g06310v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03209">
        <property type="protein sequence ID" value="KQK03209"/>
        <property type="gene ID" value="BRADI_2g06310v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JXL1"/>
    <dbReference type="OrthoDB" id="10254665at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017423">
        <property type="entry name" value="TRM6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945:SF0">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT TRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04189">
        <property type="entry name" value="Gcd10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038170">
        <property type="entry name" value="tRNA_m1A_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="79"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK03209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="433" mass="47125" checksum="3A5BA3C8369190D7" modified="2016-01-20" version="1">MQPIFKAGSAEAWKGCSVILDINDGDRVIFSRLTPRATVDIGGRKCSLQPLVGRPFGSTFRVEPCDSEGGDYSGVLVSYATDAPSRDDDPTQDGKEHERRDNRSLVDNNTAQTLSSDDIEAMKRNGLSGDAIVEALIANSSTFGKKTMQSQEKYILKKQKKYAPKLLLRRPSARSICETYLKQNADRTGFMRVDSLSLLLSMANVGAYSDVLVVDMVGGLVVGAVAERLGGTGYVCRTYLGTVPSSIDIVGIFNFSSDVTSRIVQSPLSDLYSLRNSGNAPCVLNDSIQGEADEPSAASDCKRDDCASVASTSAKAGMAPSSERMKYWREHGFSSLIVAAPEYELGSYVSDLLPLLSYSAPFAIYHRCLEPLATCMHSLVVSEMAVGLQISEPWLREYQVLPSRTHPHMKMNSFGGYILSGIWVRKPEAYSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3L1Q0</accession>
    <name>A0A0Q3L1Q0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AT-hook motif nuclear-localized protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g32901v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The PPC domain mediates interactions between AHL proteins.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3L1Q0"/>
    <dbReference type="STRING" id="15368.A0A0Q3L1Q0"/>
    <dbReference type="EnsemblPlants" id="KQK17177">
        <property type="protein sequence ID" value="KQK17177"/>
        <property type="gene ID" value="BRADI_1g32901v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17177">
        <property type="protein sequence ID" value="KQK17177"/>
        <property type="gene ID" value="BRADI_1g32901v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3L1Q0"/>
    <dbReference type="OrthoDB" id="2014829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039605">
        <property type="entry name" value="AHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31500:SF48">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31500">
        <property type="entry name" value="AT-HOOK MOTIF NUCLEAR-LOCALIZED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PPC" evidence="3">
        <location>
            <begin position="137"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="12"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="75"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK17177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="288" mass="29018" checksum="AFD5F8A332713E03" modified="2016-01-20" version="1">MSAEATYGVGLGLGIDLNKSPPKAELQLQSTVGAPADSPAQPPEPPLQTCSAPAKSASVDPRKYSLPLAILPAPQPAGAAPALPPGLANPQLVPPALSSPLQPSPSGNSKQRVKKRGRPPSSTGRTGPKVPATGSGVTGLYPQVIIVQAGEDAAEKIMSFSGNGWAVCIMSAIGAVSNVTVRASASFSPQTITYEGRFEILSLSGSYVPIELDGVSTGTGGLNIILSCTDGRLLSGGVAGPLIAASPVQVVIGRFQGDDKKQLGQAGTTETMGAMNGGSHGELRIVPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LBD3</accession>
    <name>A0A0Q3LBD3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g27732v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LBD3"/>
    <dbReference type="EnsemblPlants" id="KQJ89781">
        <property type="protein sequence ID" value="KQJ89781"/>
        <property type="gene ID" value="BRADI_4g27732v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89781">
        <property type="protein sequence ID" value="KQJ89781"/>
        <property type="gene ID" value="BRADI_4g27732v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LBD3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ89781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="160" mass="16020" checksum="2F553055EE8B5702" modified="2016-01-20" version="1">MLGMSRKEVTAAAGKDRRCSWRGAAAETASGRGAGAVAAVPGDNVGAAHGAARRQGRFLLPAREGAAQPPGVPPLHRLWLRLRRLAAAPLPPQIVLACRSGGVAAGGKGRAAVVRTAASLGGSRGGGWRLGKEQSAAADRSGAREQTRGGGGLRGGPARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LIJ2</accession>
    <name>A0A0Q3LIJ2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC104584800</name>
        <name evidence="2" type="ORF">BRADI_4g43481v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92373.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238767.1">
        <property type="nucleotide sequence ID" value="XM_010240465.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758756.1">
        <property type="nucleotide sequence ID" value="XM_014903270.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318978.1">
        <property type="nucleotide sequence ID" value="XM_024463210.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318979.1">
        <property type="nucleotide sequence ID" value="XM_024463211.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318980.1">
        <property type="nucleotide sequence ID" value="XM_024463212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LIJ2"/>
    <dbReference type="STRING" id="15368.A0A0Q3LIJ2"/>
    <dbReference type="EnsemblPlants" id="KQJ92373">
        <property type="protein sequence ID" value="KQJ92373"/>
        <property type="gene ID" value="BRADI_4g43481v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584800"/>
    <dbReference type="Gramene" id="KQJ92373">
        <property type="protein sequence ID" value="KQJ92373"/>
        <property type="gene ID" value="BRADI_4g43481v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584800"/>
    <dbReference type="OrthoDB" id="656344at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LIJ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ92373.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="485" mass="50931" checksum="ABD97968273CE25A" modified="2018-03-28" version="1">MDQLMSRSRSFAEAMVVMICPVLLALALDKVELNSKLYGRVIPALMLAIAAAALITGICPLLACCFSETGVIPNAFFFFLGQPRPAPGVVAATTTLLAVPFASGCVLVLSCWFAQLVVDRWIAIAVCSAVAICVMARTAYYHHFERNYHDLQPIDNRSSEFYDMLNQSHEFLCGVTGIFFLGLETLALQGHEEVTSKAAAGNGRMVTAAAVGLGAPMGVLIVIVCSLGVFLMLFAMIPPEPFHKDGEIAVFTLALDSIMAAATLTVLVAIVHKLMGTVAFFLPGPLLAFFVYYLFLIMTTNPGPATPATAAAAVAPTTPPPPPPAAAAGGNDIKLASLELTRVTFTGFLAVSVAALATGAANDSPSKLVITNMFLLFAAGAIVFGLSWRLLSQMQVRTLGPGADLASSVNLASFCAHLCIVIATVLFTVMAGIGGTAGRNRSNPGAPAPDMHLHQSHAVLSSAKMFAPANVITGFTSVIFCFSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ND65</accession>
    <name>A0A0Q3ND65_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g20165v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant dirigent protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ND65"/>
    <dbReference type="STRING" id="15368.A0A0Q3ND65"/>
    <dbReference type="EnsemblPlants" id="KQK15022">
        <property type="protein sequence ID" value="KQK15022"/>
        <property type="gene ID" value="BRADI_1g20165v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15022">
        <property type="protein sequence ID" value="KQK15022"/>
        <property type="gene ID" value="BRADI_1g20165v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3ND65"/>
    <dbReference type="OrthoDB" id="674745at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46442">
        <property type="entry name" value="DIRIGENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46442:SF6">
        <property type="entry name" value="DIRIGENT PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK15022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="159" mass="17374" checksum="3A51EE97172CD364" modified="2016-01-20" version="1">MKMRVYYHDILLYDAVYNSDAANATSATAPSPLSPTGFFGMMVVFNDPTKGKALPPAAVGGDEEVARAHGLYFYNRKDKFSAWFAMSLVFNGATGGGRRGTLELMGADLMDRKTRDISVVGGTGDFFLARGVATLRTDAVEGVIYYRLQMDIKLSQCYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PEF1</accession>
    <name>A0A0Q3PEF1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g12831v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PEF1"/>
    <dbReference type="FunCoup" id="A0A0Q3PEF1">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3PEF1"/>
    <dbReference type="EnsemblPlants" id="KQJ87665">
        <property type="protein sequence ID" value="KQJ87665"/>
        <property type="gene ID" value="BRADI_4g12831v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87665">
        <property type="protein sequence ID" value="KQJ87665"/>
        <property type="gene ID" value="BRADI_4g12831v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3PEF1"/>
    <dbReference type="OrthoDB" id="691578at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3PEF1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF282">
        <property type="entry name" value="OS04G0433000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="2">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ87665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="194" mass="21802" checksum="7D6CAF7F5E1A2213" modified="2016-01-20" version="1">MTITSGFHLLRIDGHSQTKNIPPGQKLSSQGFAIGGHSWRLDYYPTGLATAPNPGSAVSVYLQLMTHNVKKLLQARYSFSILDQSGATAYELPASTGTFAGLERREGLVRDDALVLRCDVQGVTEIATGLLAHDDLHGFGYGSDDDTDDEDEQYPTFGAPRHRRRRRQHRRPDDREYVKWCMAQRPAEPPRRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGP7</accession>
    <name>A0A2K2CGP7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g11912v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CGP7"/>
    <dbReference type="EnsemblPlants" id="PNT61200">
        <property type="protein sequence ID" value="PNT61200"/>
        <property type="gene ID" value="BRADI_5g11912v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61200">
        <property type="protein sequence ID" value="PNT61200"/>
        <property type="gene ID" value="BRADI_5g11912v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CGP7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT61200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="120" mass="10700" checksum="B2E1D4295AD1A0CD" modified="2018-03-28" version="1">MSGVGAGGEDIGASAGAGAAAEGVGAVSGAGTGAAVGGGDTGAGVGGGKTGAGAGGGVAGGGGGAWGAAAGAAPGACAEADTTRSARTERKASGEAAIVLVIGVLRWLRFASRLLCLRCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CIA8</accession>
    <name>A0A2K2CIA8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BRADI_5g20365v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CIA8"/>
    <dbReference type="STRING" id="15368.A0A2K2CIA8"/>
    <dbReference type="EnsemblPlants" id="PNT61759">
        <property type="protein sequence ID" value="PNT61759"/>
        <property type="gene ID" value="BRADI_5g20365v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61759">
        <property type="protein sequence ID" value="PNT61759"/>
        <property type="gene ID" value="BRADI_5g20365v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CIA8"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CIA8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034052">
        <property type="term" value="P:positive regulation of plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF168">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="31"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="218"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00058961"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="PNT61759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="60538" checksum="5CF0D38A3F053A73" modified="2018-03-28" version="1">MQGTMGTRAGYLCNCNAGYYGNPYTADGCLDINECRNNESNSCIHRDFCNNTEGGFTCSCPQNMIGDGYRTGTGCTEAPIPSGSPQQQPQGLNPCTHPETNPCEYPKYCSDGQGDVWCDCPQGMSGDGRKKGRGCQKHFPIDIVLGVGLALVVTVTTTALCYYWSMKRREARRKRAELFRKNGGLLLQQRFSAITSQGKDSSAKIFSAEELKTATNNYSESRVLGRGGYGTVYKGVLPDETVVAVKKSRVFDESQVEQFVNEITILSQIDHPNVVKLLGCCLETQVPLLVYEFIPNGTLFQHIHNRSPPHSLTWEDTLRIAAETAEALAYLHSTSSIPIIHRDIKSSNILLDENFVAKISDFGASRSVPFDQTHVTTLIQGTIGYLDPEYFQSSMLTEKSDVYSFGVVLAELLTRQKPISVGRSEESCNLAMHVVILFTEGCLLQEIEPHILEEAGEEQLYAVAHLSVRCLNLSGQERPVMKEVASVLNKLRRSFAKEQTMRRKDEPVHKNNELVHEAIPISSLHCSEGSTQFSMEAEMIASSDSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CU59</accession>
    <name>A0A2K2CU59_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g44385v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CU59"/>
    <dbReference type="EnsemblPlants" id="PNT65559">
        <property type="protein sequence ID" value="PNT65559"/>
        <property type="gene ID" value="BRADI_4g44385v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65559">
        <property type="protein sequence ID" value="PNT65559"/>
        <property type="gene ID" value="BRADI_4g44385v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CU59"/>
    <dbReference type="OrthoDB" id="1883168at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT65559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="134" mass="15436" checksum="501554B8B0CD916C" modified="2018-03-28" version="1" fragment="single">PNAGRNPPPPQERKNRTDAGISRRRNGPGPTARGPNNNAPHQRVRASFAPSRPRSGQETPRRRRRQSQHHGGRRPALQGGANGRGRGRASAAPARRRRRCFARRRRRIEGRHRLRRPARSRRIDQRRSQELARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CXQ9</accession>
    <name>A0A2K2CXQ9_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BRADI_3g17305v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CXQ9"/>
    <dbReference type="EnsemblPlants" id="PNT66814">
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        <property type="gene ID" value="BRADI_3g17305v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66814">
        <property type="protein sequence ID" value="PNT66814"/>
        <property type="gene ID" value="BRADI_3g17305v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CXQ9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="87" mass="9980" checksum="2F19CFD013D7520A" modified="2018-03-28" version="1">MFRLSADHHALHPLTSGRFDLDIEDSSSPCSWYALLHGSMVFVLPLSNFCNFYLIDMFIGLLFVRNEGAVLASNLYYLNNKTVIWYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CZ51</accession>
    <name>A0A2K2CZ51_BRADI</name>
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CZ51"/>
    <dbReference type="EnsemblPlants" id="PNT67307">
        <property type="protein sequence ID" value="PNT67307"/>
        <property type="gene ID" value="BRADI_3g24425v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67307">
        <property type="protein sequence ID" value="PNT67307"/>
        <property type="gene ID" value="BRADI_3g24425v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CZ51"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036043286" evidence="2">
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            <end position="150"/>
        </location>
    </feature>
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        <location>
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            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT67307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="150" mass="15497" checksum="6F8941EB44470FEB" modified="2018-03-28" version="1">MGQRRWARRGLGWAWASRACTGLRAGGRGGWWGALQAGGAERWRRSGRVDVRGMEELRCEAAPAGVAGGGEAVVGEQGTPGERSRRAGRRGGDEETRGRTRAEEGAGAVGGESGAGEERGGAGGGVAGGELDGARWRSKAARGERKRRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CZ65</accession>
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    </protein>
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CZ65"/>
    <dbReference type="FunCoup" id="A0A2K2CZ65">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CZ65"/>
    <dbReference type="EnsemblPlants" id="PNT67322">
        <property type="protein sequence ID" value="PNT67322"/>
        <property type="gene ID" value="BRADI_3g24743v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67322">
        <property type="protein sequence ID" value="PNT67322"/>
        <property type="gene ID" value="BRADI_3g24743v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CZ65"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
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    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF6">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT67322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="86" mass="9676" checksum="DE52BDA275B4ACE6" modified="2018-03-28" version="1">MGPGTMDVIIHQYLILPVYLGGETDDKVVEENVKKLKITFEVYEARLAKFKYLAGDFFSLADLSHFPIAHYLLATPHASLLEGLDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D307</accession>
    <name>A0A2K2D307_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="1">
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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                <person name="Harmon-Smith M."/>
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                <person name="Bruce D."/>
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                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="3">cv. Bd21</strain>
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</entry>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
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                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Rokhsar D."/>
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        <source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
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                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
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        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        </location>
    </feature>
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        <location>
            <begin position="360"/>
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        </location>
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            <begin position="1"/>
            <end position="12"/>
        </location>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT70738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="444" mass="48084" checksum="B39869A52F676C4F" modified="2018-03-28" version="1">MLISNTPPPAPAPASSIDEGAPPTPATALASTPTPTFVRSPALEEHNFSSGNSSGGSLPLWGSKKRSRAFASSSSKSPAPTQPPPVPASVTTFKPSNSAGFIPAPEAAREALLGRTWQFPHQRERRVRSEQSAPVARQPPQEPAPEMRQPQQQQPVFVARQPARELPGRQQPPREAARKGRTAPTAAEKGKAIVMPTASTVPKKRRTATEAGNDVAQTLAAMSAQRKLILPKFRLELSMDQKEEDFMLMTGGKLPCRPMKHPRTVDQNKIKHKAACCCSLPSSCCSGAARPPPLLRGEKGRDRGVRRATAACVCPPPELLAAPHRLCCGRPPAPLRRRARYPAPPRTACLPLLPMTAAPPRRRPCSSRHLPPHARSVGRSRVTEREGERARAAAACPAAESAPCYRASRRRCRPSLRRRRPQPLPPLASPQHLARRPSPHRGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">Bd21</strain>
        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="BRADI_2g30530v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1166350at2759"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DB89">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A2K2DK42</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g19285v3"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DK42"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
    </feature>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT74642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="183" mass="19252" checksum="3D1678EB354C7C7A" modified="2018-03-28" version="1">MSAAGPGRAEELTHGHASKKIKPCPGHGRHHRHSPDPSLLHLPSTAGSLLQEIAGTRRRAEIEWPNSSGGYLNAQAQPGWTGGPCPGCRKAGRCPCPQQYTRPSLAPSRCRAPQQSPRTAARLPPRASAATPPHPALPSAQPPSSSSSSRERRPPSLPRFLAPSPLARLPPRASASAAGWSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DP42</accession>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
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    <organism evidence="1">
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Harmon-Smith M."/>
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                <person name="Vogel J.P."/>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
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            <title>The complete chloroplast genome sequence of Brachypodium distachyon: sequence comparison and phylogenetic analysis of eight grass plastomes.</title>
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                <person name="Coleman-Derr D."/>
                <person name="Lazo G.R."/>
                <person name="Anderson O.D."/>
                <person name="Gu Y.Q."/>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            <dbReference type="PubMed" id="20148030"/>
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    <reference evidence="8" key="3">
        <citation type="journal article" date="2017" name="New Phytol." volume="0" first="0" last="0">
            <title>Comparative plastome genomics and phylogenomics of Brachypodium: flowering time signatures, introgression and recombination in recently diverged ecotypes.</title>
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                <person name="Cantalapiedra C.P."/>
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                <person name="Gordon S."/>
                <person name="Vogel J.P."/>
                <person name="Catalan P."/>
                <person name="Contreras-Moreira B."/>
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            <dbReference type="DOI" id="10.1111/nph.14926"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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        <citation type="submission" date="2019-03" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="component" value="Chromosome 5"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.610:FF:000001">
        <property type="entry name" value="30S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.610">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_uS2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACF08633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CZT22477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ84934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="VGO04200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="236" mass="26895" checksum="134D6A8B4CEC77C5" modified="2008-09-02" version="1">MTRRYWNINLKEMIEAGVHFGHGIKKWNPKMAPYISAKRKGTHITNLARTARFLSEACDLVFDAASQGKSFLIVGTKKRAADLVASAAIRARCHYVNKKWFSGMLTNWSITKTRLSQFRDLRAEEKMGKFHHLPKRDVAILKRKLSTLQRYLGGIQYMTRLPDIVIVLDQQKEYIALRECAILGIPTISLVDTNCDPDLANISIPANDDTMTSIRLILNKLVFAICEGRSLYIRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLZ8</accession>
    <name>I1GLZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Squalene synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100825967</name>
        <name evidence="3" type="ORF">BRADI_1g04900v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phytoene/squalene synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559279.1">
        <property type="nucleotide sequence ID" value="XM_003559231.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLZ8"/>
    <dbReference type="STRING" id="15368.I1GLZ8"/>
    <dbReference type="EnsemblPlants" id="KQK12612">
        <property type="protein sequence ID" value="KQK12612"/>
        <property type="gene ID" value="BRADI_1g04900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825967"/>
    <dbReference type="Gramene" id="KQK12612">
        <property type="protein sequence ID" value="KQK12612"/>
        <property type="gene ID" value="BRADI_1g04900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825967"/>
    <dbReference type="eggNOG" id="KOG1459">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031981_0_0_1"/>
    <dbReference type="OMA" id="DYAEDCE"/>
    <dbReference type="OrthoDB" id="431150at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GLZ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010287">
        <property type="term" value="C:plastoglobule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045338">
        <property type="term" value="P:farnesyl diphosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000012">
        <property type="entry name" value="Squalene synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006449">
        <property type="entry name" value="Squal_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044844">
        <property type="entry name" value="Trans_IPPS_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01559">
        <property type="entry name" value="squal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626">
        <property type="entry name" value="FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626:SF2">
        <property type="entry name" value="SQUALENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK12612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="46166" checksum="3C4CD8CD78CD477E" modified="2012-06-13" version="1">MEAVWEAVALVKLRVAAGRVKLQIPPQEEEEHWAFAYDMLQKVSRSFAFVIQQLGPDLRNAVCIFYLLLRALDTVEDDTSIPTEVKLTILQEFHRHVYNPSWHYSCGTGNYRVLMDKFRYVSTAFLELDQGCQEAIKEITKRMGEGMSKFICKEVETVDDYDEYCHYAAGLVGYGLSRLFHAAGTENLASDQLSNSMGLFLQKIHIIGDYFEDISEIPKRRMFWPREIWSKYVEKLEDLKYKENSEKAVQCLNDMVTNALIHAKDCLQYMSALKDDTVFRLCAIPQIMAMGNFAICYNNVNVFRGLVKMRHGLTARVIDETKSMSDVYTAFYEFSSLLESKIDDNDPSAALTRKRVDAIKKTCKSSGLLKRRGYDLEKPKYMPVLIMLALMLAAVVLGVLYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMN8</accession>
    <name>I1GMN8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100831481</name>
        <name evidence="3" type="ORF">BRADI_1g06830v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228983.1">
        <property type="nucleotide sequence ID" value="XM_010230681.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMN8"/>
    <dbReference type="EnsemblPlants" id="KQK12911">
        <property type="protein sequence ID" value="KQK12911"/>
        <property type="gene ID" value="BRADI_1g06830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831481"/>
    <dbReference type="Gramene" id="KQK12911">
        <property type="protein sequence ID" value="KQK12911"/>
        <property type="gene ID" value="BRADI_1g06830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831481"/>
    <dbReference type="eggNOG" id="KOG0001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173745_0_0_1"/>
    <dbReference type="OMA" id="AHEPFHM"/>
    <dbReference type="OrthoDB" id="767900at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK12911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7425" checksum="6EAF6590D902668B" modified="2012-06-13" version="1">MGFVLVISLPFIFFTVLLGFGCYFFGKHRGREEMRTGVGAQIYGTPLPPPGAPAPVPPFPMKNEGPDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNY5</accession>
    <name>I1GNY5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100824436</name>
        <name evidence="3" type="ORF">BRADI_1g10580v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559494.1">
        <property type="nucleotide sequence ID" value="XM_003559446.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNY5"/>
    <dbReference type="STRING" id="15368.I1GNY5"/>
    <dbReference type="EnsemblPlants" id="KQK13504">
        <property type="protein sequence ID" value="KQK13504"/>
        <property type="gene ID" value="BRADI_1g10580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824436"/>
    <dbReference type="Gramene" id="KQK13504">
        <property type="protein sequence ID" value="KQK13504"/>
        <property type="gene ID" value="BRADI_1g10580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824436"/>
    <dbReference type="eggNOG" id="ENOG502R61J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055715_2_1_1"/>
    <dbReference type="OMA" id="CLCATIR"/>
    <dbReference type="OrthoDB" id="641595at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01958">
        <property type="entry name" value="HPS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027923">
        <property type="entry name" value="Hydrophob_seed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051636">
        <property type="entry name" value="Plant_LTP/defense-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31731">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14547">
        <property type="entry name" value="Hydrophob_seed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093955" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK13504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="121" mass="12375" checksum="8EC684ABE7DF1EC0" modified="2012-06-13" version="1">MAACKPRALAALFVALNLLVVSGVVRGQTPSSRGNPCPTNALADLKVCGDVLVLLKLKINVPANQQCCPLIGQLVKLDVAACLCAAIKLSVLGIPINLPLDVPLVLNYCGRNASAAGANCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQ04</accession>
    <name>I1GQ04_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="12 13">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100836219</name>
        <name evidence="16" type="ORF">BRADI_1g13710v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="12">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the PPP phosphatase family. BSU subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12 13"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561867.1">
        <property type="nucleotide sequence ID" value="XM_003561819.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQ04"/>
    <dbReference type="STRING" id="15368.I1GQ04"/>
    <dbReference type="EnsemblPlants" id="KQK13977">
        <property type="protein sequence ID" value="KQK13977"/>
        <property type="gene ID" value="BRADI_1g13710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836219"/>
    <dbReference type="Gramene" id="KQK13977">
        <property type="protein sequence ID" value="KQK13977"/>
        <property type="gene ID" value="BRADI_1g13710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836219"/>
    <dbReference type="eggNOG" id="KOG0374">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_7_0_1"/>
    <dbReference type="OMA" id="NDELQWR"/>
    <dbReference type="OrthoDB" id="309851at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07419">
        <property type="entry name" value="MPP_Bsu1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000042">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000139">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000008">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041758">
        <property type="entry name" value="MPP_BSL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012391">
        <property type="entry name" value="Ser/Thr_prot_Pase_BSU1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE BSL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE BSL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036363">
        <property type="entry name" value="PPP_BSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="8 12" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="15">
        <location>
            <begin position="759"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="539"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="540"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR036363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK13977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="993" mass="105316" checksum="8F3436540D561F8C" modified="2012-06-13" version="1">MDVDSRMTTESDSDSDATTAAAALGRGSGSETSSSSAPSTPGTPTAAPASPAVAGSGPRPAPGYTAVTAVIEKKEDGPGCRCGHTLTSVPAVGEEGSQSYVGPRLILFGGATALEGNSATPPSSAGSAGIRLAGATADVHCYDVLSNKWSRLTPLGEPPSPRAAHVATAVGTMVVIQGGIGPAGLSAEDLHVLDLTQQRPRWHRVVVQGPGPGPRYGHVMALVGQRFLLTIGGNDGKRPLADVWALDTAAKPYEWRKLEPEGEGPPPCMYATASARSDGLLLLCGGRDTNSVPLSSAYGLAKHRDGRWEWAIAPGVSPSPRYQHAAVFVNARLHVSGGALGGGRMVEDSSSVAVLDTAAGVWCDIKSVVTTPRTGRYSADAAGGDAAVELTRRCRHAAAAVGDLIFIYGGLRGGVLLDDLLVAEDLAAAETTTAANHAAASAAATDIQAGRVPGRYAYNDDRARQAAPESAPDGSVVLGTPVAPPVNGDMYTDISPENAVLQGQRRLSKGVDYLVEASAAEAEAISATLAAVKARQVNGETDHLSDKEESPDSTSSSKHSSLIKPDNALSNMTPPPGVRLHHRAVVVAAETGGALGGMVRQLSIDQFENEGRRVSYGTPENATAARKLLDRQMSINSIPKKVIASLLKPRGWKPPVRRQFFLDCNEIADLCDSAERIFSSEPSVLQLKAPIKIFGDLHGQFGDLMRLFDEYGAPSTAGDIAYIDYLFLGDYVDRGQHSLETMSLLLALKVEYPQNVHLIRGNHEAADINALFGFRIECIERMGERDGIWTWHRMNRLFNWLPLAALIEKKIICMHGGIGRSINHVEQIENLQRPITMEAGSVVLMDLLWSDPTENDSVEGLRPNARGPGLVTFGPDRVMEFCNNNDLQLIVRAHECVMDGFERFAQGHLITLFSATNYCGTANNAGAILVLGRDLVVVPKLIHPLPPAITSPETSPDHIEDTWMQELNANRPATPTRGRPQAAANDRGALAWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I1GR40</accession>
    <name>I1GR40_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100828359</name>
        <name evidence="15" type="ORF">BRADI_1g17630v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562446.1">
        <property type="nucleotide sequence ID" value="XM_003562398.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233881.1">
        <property type="nucleotide sequence ID" value="XM_010235579.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754679.1">
        <property type="nucleotide sequence ID" value="XM_014899193.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754696.1">
        <property type="nucleotide sequence ID" value="XM_014899210.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GR40"/>
    <dbReference type="STRING" id="15368.I1GR40"/>
    <dbReference type="EnsemblPlants" id="KQK14619">
        <property type="protein sequence ID" value="KQK14619"/>
        <property type="gene ID" value="BRADI_1g17630v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK14620">
        <property type="protein sequence ID" value="KQK14620"/>
        <property type="gene ID" value="BRADI_1g17630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828359"/>
    <dbReference type="Gramene" id="KQK14619">
        <property type="protein sequence ID" value="KQK14619"/>
        <property type="gene ID" value="BRADI_1g17630v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14620">
        <property type="protein sequence ID" value="KQK14620"/>
        <property type="gene ID" value="BRADI_1g17630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828359"/>
    <dbReference type="eggNOG" id="KOG0598">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_49_1"/>
    <dbReference type="OMA" id="VHTMNER"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GR40">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000297">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000048">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="152"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="408"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK14619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="485" mass="53807" checksum="4329210E26CE0DFA" modified="2012-06-13" version="1">MVSSQISSLTIKLAHGPKTFTRNILLPMDPPNASSSENAEYDFSDVFGSSPVQMAENLCLLGPDSPAAPIESNEEVYNDPVVIIKRSHSLVGPTSLVSCSLGLDKLTLSKAEGSPEPVDCTTEENEVNLEQLSDEEFGNAMTEDEGVGLDDFEILKLVGQGAFGKVFQVRKKGTSEIYAMKVMRKDKILEKNHSEYMKAERDILTKVDHPFVVQLRYSFQTKYRLYLVLDFINGGHLFFQLYKQGLFREELARIYTAEIVSAVSHLHANGIMHRDLKPENILLDADGHAMLTDFGLAKEFRENTRSNSICGTLEYMPPEIILGQGHDKAADWWSVGILLFEMVTGKPPFVGNREKIQQKIVKEKLKLPPFLSSEAHSLLKGLLNKDAAKRLGTGPGGSDEIKKHKWFKPINWRKLEAREIQPSFRPNVAGLTCIANFDACWTNTSVLDSPAATPVTAGGGQGNFPGFTYVRPTPFLRELKPSTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTV3</accession>
    <name>I1GTV3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100829856</name>
        <name evidence="8" type="ORF">BRADI_1g25950v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560138.1">
        <property type="nucleotide sequence ID" value="XM_003560090.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GTV3"/>
    <dbReference type="FunCoup" id="I1GTV3">
        <property type="interactions" value="481"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GTV3"/>
    <dbReference type="EnsemblPlants" id="KQK15948">
        <property type="protein sequence ID" value="KQK15948"/>
        <property type="gene ID" value="BRADI_1g25950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829856"/>
    <dbReference type="Gramene" id="KQK15948">
        <property type="protein sequence ID" value="KQK15948"/>
        <property type="gene ID" value="BRADI_1g25950v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829856"/>
    <dbReference type="eggNOG" id="ENOG502T258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050605_4_0_1"/>
    <dbReference type="OMA" id="RMYVFHG"/>
    <dbReference type="OrthoDB" id="674678at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF26">
        <property type="entry name" value="OS07G0524400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="7">
        <location>
            <begin position="70"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK15948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="209" mass="21962" checksum="DA09AA8704BA7A64" modified="2012-06-13" version="1">MGTKARKKHHTLSCCVLLAILAILGVLAIVFYILYRPLPPRVVATPVVIGVEHFGLLPVPSLTLSVGVHVVVSNPSRAPFRYEETSTPVLYHGEPVGVTVVPAGRVGGYGTSTVEPLTEVDGVKVAADPHFAADVAVGEGALPFVAFVRLDGKALVLRVFEVSVTVEVVCYVRVMVFHGESSSRCVASVRTGSSGGGEGRESVAPLHVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>I1GU02</accession>
    <name>I1GU02_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC100824051</name>
        <name evidence="21" type="ORF">BRADI_1g26310v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">Bd21</strain>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312381.1">
        <property type="nucleotide sequence ID" value="XM_024456613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GU02"/>
    <dbReference type="STRING" id="15368.I1GU02"/>
    <dbReference type="EnsemblPlants" id="KQK16016">
        <property type="protein sequence ID" value="KQK16016"/>
        <property type="gene ID" value="BRADI_1g26310v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16017">
        <property type="protein sequence ID" value="KQK16017"/>
        <property type="gene ID" value="BRADI_1g26310v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16018">
        <property type="protein sequence ID" value="KQK16018"/>
        <property type="gene ID" value="BRADI_1g26310v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824051"/>
    <dbReference type="Gramene" id="KQK16016">
        <property type="protein sequence ID" value="KQK16016"/>
        <property type="gene ID" value="BRADI_1g26310v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16017">
        <property type="protein sequence ID" value="KQK16017"/>
        <property type="gene ID" value="BRADI_1g26310v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16018">
        <property type="protein sequence ID" value="KQK16018"/>
        <property type="gene ID" value="BRADI_1g26310v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_37_3_1"/>
    <dbReference type="OMA" id="CMELCAG"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009931">
        <property type="term" value="F:calcium-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000015">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000249">
        <property type="entry name" value="Calcium-dependent protein kinase SK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="84"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="384"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="420"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="456"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="494"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQK16016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="532" mass="59379" checksum="0A80E00371E8E64A" modified="2012-06-13" version="1">MGQCCSRATSPDSGRGVNGYGYSHQPKQAQTPPSYNHAHPPPQAEVRYTPPAMNPPVVPPVVAPSKPTPDTILGKQYEDVRSVYSLGKELGRGQFGVTYLCTEIATGRQYACKSISKRKLVSKADKEDIRREIQIMQHLSGQPNIVEFCGAYEDKSSVHVVMELCAGGELFDRIIAKGHYSERAAATICRGVVNVVNVCHFMGVMHRDLKPENFLLATKDENAVLKATDFGLSVFIEEGKMYRDIVGSAYYVAPEVLKRNYGKEIDVWSAGVILYILLSGVPPFWAETEKGIFDAILQGDIDFESQPWPSISESAKDLVRKMLAQDPKKRINSAQVLQHPWLREGEASDKPIDSAVLSRMKQFRAMNKLKKMALKVIASNLNEEEIKGLKQMFSNMDTDNSGTITYEELKAGLAKLGSKLSEAEVKQLMDAADVDGSGSIDYVEFITATMHRHKLERDEHLFKAFQYFDKDSSGFITRDELETALIEHEMGDTDTIKDIISEVDTDNDGRINYDEFCAMMRGGIQQQPVRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1GUT1</accession>
    <name>I1GUT1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100844653</name>
        <name evidence="11" type="ORF">BRADI_1g28760v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563177.1">
        <property type="nucleotide sequence ID" value="XM_003563129.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GUT1"/>
    <dbReference type="SMR" id="I1GUT1"/>
    <dbReference type="FunCoup" id="I1GUT1">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GUT1"/>
    <dbReference type="EnsemblPlants" id="KQK16441">
        <property type="protein sequence ID" value="KQK16441"/>
        <property type="gene ID" value="BRADI_1g28760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844653"/>
    <dbReference type="Gramene" id="KQK16441">
        <property type="protein sequence ID" value="KQK16441"/>
        <property type="gene ID" value="BRADI_1g28760v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844653"/>
    <dbReference type="eggNOG" id="KOG0223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_3_0_1"/>
    <dbReference type="OMA" id="LFITIAW"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GUT1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000001">
        <property type="entry name" value="Probable aquaporin PIP1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45687">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK16441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="290" mass="30438" checksum="77E41C27B3D04E10" modified="2012-06-13" version="1">MAKDEVMESGGGHQDFAAKDYTDPPPAPLIDAAELASWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDVSASGPDAACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYMIAQCLGAMCGVGLVKAFQSAYFVRYGGGANTLAAGYSKGTGLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIFNKDKAWDDQWIFWVGPMVGAAIAAFYHQYILRAGAIKALGSFRSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1GVN5</accession>
    <name>I1GVN5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100826831</name>
        <name evidence="9" type="ORF">BRADI_1g31170v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563372.1">
        <property type="nucleotide sequence ID" value="XM_003563324.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GVN5"/>
    <dbReference type="STRING" id="15368.I1GVN5"/>
    <dbReference type="EnsemblPlants" id="KQK16870">
        <property type="protein sequence ID" value="KQK16870"/>
        <property type="gene ID" value="BRADI_1g31170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826831"/>
    <dbReference type="Gramene" id="KQK16870">
        <property type="protein sequence ID" value="KQK16870"/>
        <property type="gene ID" value="BRADI_1g31170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826831"/>
    <dbReference type="eggNOG" id="KOG0265">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_2_1"/>
    <dbReference type="OMA" id="IWDIRPY"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000229">
        <property type="entry name" value="Small nuclear ribonucleoprotein U5 subunit 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="50"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="93"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="135"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="184"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="219"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="280"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="311"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK16870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="344" mass="38292" checksum="C5BED46E81164B74" modified="2012-06-13" version="1">MFSAPGNNSLALAAPRPGMELGNFHQHPNQALGPGGKQRTSSLEAPIMLLTGHQSAVYCMKFNPAGTVVASGSHDKDIFLWYVHGDCKNYMVLRGHRNAILDLQWTTDGTQIISASPDKTLRVWDVETGKQVKKMAEHSSFVNSCCPARKWPPLVVSGSDDGTAKLWDLRQRGAIQTLPDDYQITAVSFSEAADKVFTGGLDNDVKWWDLRKNEVTEHLKGHQDMITGMQLSPDGSYLLTNAMDNELKIWDLRPYAPENRNIKTLTGHQHNFEKNLLKCSWSPDNRKVTAGSADRMVYIWDTTSRRILYKLPGHNGSVNETAFHPTEPIIGSCGSDKQIYLGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWI0</accession>
    <name>I1GWI0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100843726</name>
        <name evidence="5" type="ORF">BRADI_1g33680v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560501.1">
        <property type="nucleotide sequence ID" value="XM_003560453.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWI0"/>
    <dbReference type="STRING" id="15368.I1GWI0"/>
    <dbReference type="EnsemblPlants" id="KQK17323">
        <property type="protein sequence ID" value="KQK17323"/>
        <property type="gene ID" value="BRADI_1g33680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843726"/>
    <dbReference type="Gramene" id="KQK17323">
        <property type="protein sequence ID" value="KQK17323"/>
        <property type="gene ID" value="BRADI_1g33680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843726"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_37_2_1"/>
    <dbReference type="OMA" id="GLDQSCY"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002936">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001027">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At5g44230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000515">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF523">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="312"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="343"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="560"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK17323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="652" mass="71000" checksum="32367A6AEEC9CBB5" modified="2012-06-13" version="1">MVHLAPPSLPTARLHAPAAPPLPGTLPLALLPPPTLASLLLAAVDSSPSLRHLRHLHALLIRLPLPPRSLPFILSRLLRRLAALPPPHAPLPYALSVFAALSPPDPFLAAALLRFALLTQPPLSPFHLFSRILRTARGGELPFLPFAFSPLAKSAAAARSLPAAQAAHAVSFLVGGFDKHRFVENSLIGAYIACGDVGAARKVFDEMVVKDVICWTSIVVAYSRSRDMGSAEEVFGQCPVKDMVAWTAMVTGYAQNAMPMKALEVFDRMTALGIGIDEVSLTGAISACAQLGAVKRAAWVQEIAEMNGFGMNVVVGSGLVDMYAKCGLIDEACKVFDGMQEKNVYTYSSMVVGLASHGRAREAIALFKDMVRRADVAPNHVTFIGVLTACSHAGMVTDGRYYFAQMKDKYGILPSADHYTCMVDLLGRAGLVDEALDLVRSMTVEPHGGVWGALLGACRIHGKTEVAKVAAEHLFKLEPEGIGNYVLLSNTLAAAGKWDEVSKVRKVMRSRRLKKVPAVSWFEGRDGLVHQFFAGDNSHPRTREIKKALLELVAKLKHAGYVPILSSIVYDVSNEEKERLLMGHSEKLALLFGLLTLGSRCTIRIVKNLRICDDCHLFIRLVSRFEPVEIIVRDNMRFHHFKDGECSCGGFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWR9</accession>
    <name>I1GWR9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100827328</name>
        <name evidence="6" type="ORF">BRADI_1g34430v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560525.1">
        <property type="nucleotide sequence ID" value="XM_003560477.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318940.1">
        <property type="nucleotide sequence ID" value="XM_024463172.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318957.1">
        <property type="nucleotide sequence ID" value="XM_024463189.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318988.1">
        <property type="nucleotide sequence ID" value="XM_024463220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWR9"/>
    <dbReference type="EnsemblPlants" id="KQK17430">
        <property type="protein sequence ID" value="KQK17430"/>
        <property type="gene ID" value="BRADI_1g34430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827328"/>
    <dbReference type="Gramene" id="KQK17430">
        <property type="protein sequence ID" value="KQK17430"/>
        <property type="gene ID" value="BRADI_1g34430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827328"/>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000837_25_4_1"/>
    <dbReference type="OMA" id="YLYLWDS"/>
    <dbReference type="OrthoDB" id="632105at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1020">
        <property type="entry name" value="OS06G0703200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="3">
        <location>
            <begin position="180"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="4">
        <location>
            <begin position="493"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="650"/>
            <end position="966"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK17430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="996" mass="112287" checksum="A4E47D31F383A320" modified="2012-06-13" version="1">MADLVAGVLSNVVLRGLESVEQGSWLWLLDEDRAVISGGPRIDIAKQELQELEDDINSASFLLAAGFGKPQPIDGWISEARKVAFCLADQIDMYAIQGSTRSELYQMKRDIGMLRIAELTTRIRDYKVEGEGTGQMPRAVRGNREEQVPQLSELSPFIRRALPELLPAEELLTITLMDSKIKRVEAFLADARSVLFITGGQGSGKTTLMNYVYASQKEKTKAVHGKALWNDFNQCCWVDVAKTGGLVDLLKKVLKESNVGCDSIHEFEMIKTVHDNFKDKNYLIVLDNVQDTSVLYSLKNMLNELKGKIICLTRRKDIQEDKNQDVLHITKLEPYESFKLFLSVVFHMSVNNDKEDFNSVLANAITDKDAHTSRSLLATDLKKMVKLLNVILSKCQDNPWNIRSIGTQLEANPLGKWNEIGNRIDGMIIDDTKKYGKRDPPVQLEDASLANAAIRHCFLYCLAFPVTSENGNETSETGNETSEIMQDSGGIPTRKLIRLWTAEGYVQDSENQEQAECLLEELIKKNLLVVKKKDFDGKVLKCSVNEHVRPLAERMCEQQKFCKVVIDDGGDEESSTSRVAKCFPISPNIRIREKKKPLSDRYRMLAVHGDRGVTSETLGKDIRLRSLLHFKTGRVEHSDNIDLSFRRTNMLLRTLDLEGARLAYLPSSIDSLVCLRYLGLRNTQLEDLPQTLPRHLLCLDIRDTHVAELADVSQFSEMRHLYLTKSFRSHSVVIREGLQSLTHLQTLSGAAYRESSGRQYSRIVPFEQELSHLRRLRKLSMKKTSSASSKNICDAINGMDFLNSLAISCEKDGRKFDLSHLKIGKNLRKLKLGGPLGKFPVLQPLLQSITFLYLWDNELDMNLLDALKGLKNLLVLVLLNASTSDKMVCDNGYEKLKKLCIMSMENLSECKFGQNAMGNLEELVFASCDKLKRPPPELEHLGSLREVLLSKMPSEFCEDAIIKKVEDKVHVLPELSYDVSKIPSHMIRGAATEGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYK1</accession>
    <name>I1GYK1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1,4-alpha-glucan branching enzyme</fullName>
            <ecNumber evidence="5">2.4.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100826523</name>
        <name evidence="10" type="ORF">BRADI_1g41970v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="5"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYK1"/>
    <dbReference type="STRING" id="15368.I1GYK1"/>
    <dbReference type="EnsemblPlants" id="KQK18365">
        <property type="protein sequence ID" value="KQK18365"/>
        <property type="gene ID" value="BRADI_1g41970v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18365">
        <property type="protein sequence ID" value="KQK18365"/>
        <property type="gene ID" value="BRADI_1g41970v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0470">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="HWDPPPE"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="UniPathway" id="UPA00152"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GYK1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009501">
        <property type="term" value="C:amyloplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019252">
        <property type="term" value="P:starch biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005983">
        <property type="term" value="P:starch catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001322">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001359">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000045">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000093">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF4">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME 3, CHLOROPLASTIC_AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="9">
        <location>
            <begin position="397"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="48"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK18365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="916" mass="105276" checksum="D4A371F4F7BCC4D5" modified="2012-06-13" version="1">MASCPLFLLVPHPPPPLVAIPRRHSPLPHILTSCRSPLLSRQQSFRCRCSSSSSSSSSSGPRERSPRPQQQQGCTQQRPGRGSAVDPVGFLAKIGVSDRAFAQFLRDRHKAFKDRKWEMHSRFIDLKEASSGFDLLGLHRHRQHRVDFMDWAPGARYCSLVGDFNQWSATENCAREGHLGHDDFGYWFVILEDKLREGQEADEYFFQEYNYVDDYDKGDNGVNAEDLIRRMNEEYWEPGEVKSRRSRLETVAKLYEQMFGPNGPQTEEELGEIPDAETRYKNWKATQKDDLSSSSPSYDIIDTGQDFDIFNVVTDRASFEKFQAKATPLAYWVEMRKGRKAWIEKYVPAISHKDKYRVYFNTPDGALERVPAWATYVLPDAEGMQSYAVHWEPPPEEIYKWRFQRPKIKGSLRIYECHVGISGSEQKISSFQEFMSSVLPHIKEAGYNAIQLIGVPEHKDYSSVGYKVTNYFAVSSRFGTPDDFKKLVDEAHGLGLLVLLDIVHSYASADELVGLSLYDGSNDCYFHSGKRGHHKYWGTRMFKYDDVDVLHFLLSNLNWWVTEYQIDGFQFHSLSSMLYTHNGFSTFTGAIEEYCNQYVDKDALIYLILANEMLHELHPDIITIAEDATYYPGLCEPTTQGGLGFDYWTNLSIPDMWLWHLENVPEREWSMSKIMKVLISSNHNMLSYVENHNQSISGRKSFAEIVLNTGMSSSGSVDDDLIFRASSLLKIIKLITFTTSGGAYLNFMGNEFAHPKRVEFPMSSNDYSFHLAYRQWELLDKGVHKNVFNFDKDIMTLDENERIISRGSLNIHHCDDTNMVISFTRGPFLFVFNFNPDVPYQLYRIGVDEAGEYQLILNTDETKYGGCGELNSSQYMKRTNDKRVDGCRNSLELTLASRSAQLDHQCQCPYSRKLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2L1</accession>
    <name>I1H2L1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100827000</name>
        <name evidence="18" type="ORF">BRADI_1g53880v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Carrier of the growing fatty acid chain in fatty acid biosynthesis. May be involved in the synthesis of short and medium chain fatty acids. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Complex I is composed of at least 49 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK20336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003557327.3"/>
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        <property type="interactions" value="1473"/>
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    <dbReference type="STRING" id="15368.I1H2L1"/>
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        <property type="protein sequence ID" value="KQK20336"/>
        <property type="gene ID" value="BRADI_1g53880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827000"/>
    <dbReference type="Gramene" id="KQK20336">
        <property type="protein sequence ID" value="KQK20336"/>
        <property type="gene ID" value="BRADI_1g53880v3"/>
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    <dbReference type="eggNOG" id="KOG1748">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="OrthoDB" id="448946at2759"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Acyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002148">
        <property type="entry name" value="PRK00982.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
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    </dbReference>
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        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 16" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 16" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="17">
        <location>
            <begin position="54"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK20336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="132" mass="14251" checksum="A9839FDA40AB94D0" modified="2012-06-13" version="1">MAAAALRPAILRHIRLSATTVTPLAAAAAAAQPHALARWLGRPMSSHADHLTHDEVVGRVLDVLKSHPKVDPSKVTPEAHFEKDLGLDSLDTVEVVMAIEEEFKLEIPDMEADKIDSLPLAIEYVANHPMAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4K1</accession>
    <name>I1H4K1_BRADI</name>
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        <recommendedName>
            <fullName evidence="6">PdxS/SNZ N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100842713</name>
        <name evidence="7" type="ORF">BRADI_1g59870v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the PdxS/SNZ family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK21272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_010228496.1">
        <property type="nucleotide sequence ID" value="XM_010230194.3"/>
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    <dbReference type="AlphaFoldDB" id="I1H4K1"/>
    <dbReference type="STRING" id="15368.I1H4K1"/>
    <dbReference type="EnsemblPlants" id="KQK21272">
        <property type="protein sequence ID" value="KQK21272"/>
        <property type="gene ID" value="BRADI_1g59870v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842713"/>
    <dbReference type="Gramene" id="KQK21272">
        <property type="protein sequence ID" value="KQK21272"/>
        <property type="gene ID" value="BRADI_1g59870v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842713"/>
    <dbReference type="eggNOG" id="KOG1606">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055352_1_0_1"/>
    <dbReference type="OMA" id="YSDPNML"/>
    <dbReference type="OrthoDB" id="1660966at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0016843">
        <property type="term" value="F:amine-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
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    </dbReference>
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        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pyridoxine biosynthesis protein PDX1"/>
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    </dbReference>
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        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00343">
        <property type="entry name" value="pyridoxal 5'-phosphate synthase lyase subunit PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF8">
        <property type="entry name" value="PDXS_SNZ N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="6">
        <location>
            <begin position="27"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="3">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="255"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR029271-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR029271-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK21272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="313" mass="33091" checksum="490F4452039BF6AC" modified="2012-06-13" version="1">MASDGSGVVALYGGNNNNSILDSKSPFAVKAGLAQMLRGGVIMDVVTADQARIAEEAGACAVMALERVPADIRAQGGVARMSDPGLIRDIKRAVTIPVMAKARIGHFVEAQILEHIGVDYVDESEVLTLADDAHHINKHNFRVPFVCGCRNLGEALRRVREGAAMIRTKGEAGTGNVVEAVRHVRSVMGDVRALRAMDDDEVFSYAKSIAAPYDLVLQTKQLGRLPVVQFAAGGVATPADAALMIQLGCDGVFVGSGIFKSGDPARRARAIVQAVTHYSDPAVLAEVSCDLGEAMVGINLSDPKVERFAARSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1H5Y9</accession>
    <name>I1H5Y9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ripening-related protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_1g63750v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kiwellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H5Y9"/>
    <dbReference type="FunCoup" id="I1H5Y9">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H5Y9"/>
    <dbReference type="EnsemblPlants" id="KQK21889">
        <property type="protein sequence ID" value="KQK21889"/>
        <property type="gene ID" value="BRADI_1g63750v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21889">
        <property type="protein sequence ID" value="KQK21889"/>
        <property type="gene ID" value="BRADI_1g63750v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ0R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047639_4_2_1"/>
    <dbReference type="InParanoid" id="I1H5Y9"/>
    <dbReference type="OMA" id="CDDNCCE"/>
    <dbReference type="OrthoDB" id="406505at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22270">
        <property type="entry name" value="DPBB_kiwellin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039271">
        <property type="entry name" value="Kiwellin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191:SF76">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24300">
        <property type="entry name" value="KWL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094364" description="Ripening-related protein 5" evidence="5">
        <location>
            <begin position="27"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK21889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="20319" checksum="7A68C3D0AC6F60F4" modified="2012-06-13" version="1">MATASTAVTMVFFILVILSASHVASSFRPGLGVCRASGYLPGKTGHCEKSNDSDCCEDGKRYPQYHCSPTVTADTKAVLTLNSFEKGKDGGGPSECDNAYHSDEEMVVALSTGWFENMGRCGHRIKITANGKSVYAKVVDECDSVYGCDEDHNYEPPCDNNIVDASPAVWNALGLDQNVGMEGITWSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6X9</accession>
    <name>I1H6X9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_1g66550v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6X9"/>
    <dbReference type="STRING" id="15368.I1H6X9"/>
    <dbReference type="EnsemblPlants" id="KQK22330">
        <property type="protein sequence ID" value="KQK22330"/>
        <property type="gene ID" value="BRADI_1g66550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22330">
        <property type="protein sequence ID" value="KQK22330"/>
        <property type="gene ID" value="BRADI_1g66550v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_0_1_1"/>
    <dbReference type="InParanoid" id="I1H6X9"/>
    <dbReference type="OMA" id="FAIEANC"/>
    <dbReference type="OrthoDB" id="3789372at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000005">
        <property type="entry name" value="Heat shock protein ssb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="471"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK22330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="481" mass="52359" checksum="87699C918D316C14" modified="2012-06-13" version="1">MAAGTNGDGPAIGIDLATTYSCVAVWRPAHNRVEVIANDQGNLTTPSCVAFTDTWRLIGESAMNPSNTIFDAKRLIGRRYKDPSVQEDIKLWPFKVISGPGERPIIVVQYRGEERPFAAEEISAMVLVKMRETAEAYLGSAVKNAVITVPVYFNGSQRQDTIDAGAIAGLNVMRIINEPSAAAIADGLDRMSGSSEVKTVLIFDLGGGTLDISVIDIENGKFIVQATSGDTHLGGEDLNSRMELNMDLFRKCIEHVEKCLGDAKMDKSQIHDVVLVGGSSRIPKVQQLLQDFFDGKMLCRNINPDETVAYGAAVQAAVLSGEYQQVQDMLLLDVTPLSLGIEVVGDIMSVVIPRITTIPCKKGRIYTTEYDNQTVAAINVYEGEGALIKDNNLLGKFTLEGIPPAPRLVPKINVTFEIEMNGILKVSAEDMTTGNKNSISIKTDKGGLTKEEIERMVQDAEKYKSEDKKNLTKIKKERGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H7Q0</accession>
    <name>I1H7Q0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100833735</name>
        <name evidence="2" type="ORF">BRADI_1g68880v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003558366.4"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.I1H7Q0"/>
    <dbReference type="EnsemblPlants" id="KQK22703">
        <property type="protein sequence ID" value="KQK22703"/>
        <property type="gene ID" value="BRADI_1g68880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833735"/>
    <dbReference type="Gramene" id="KQK22703">
        <property type="protein sequence ID" value="KQK22703"/>
        <property type="gene ID" value="BRADI_1g68880v3"/>
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    <dbReference type="eggNOG" id="KOG2659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073203_1_0_1"/>
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    <dbReference type="OrthoDB" id="2415936at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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        <property type="entry name" value="CRA_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
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        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
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    </dbReference>
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        <location>
            <begin position="94"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="259" mass="29525" checksum="6CF75D31B8001939" modified="2012-06-13" version="1">MNPFVWRRGKAEKLLSLPVLEECFCACRQLPASMASSKKVVTRDEWERKLRDVKIRKEDMNRLVMNFLVTEGFVDAADKFRIESGTQPDIDLATITDRMEVKKAVQSGNVQEAIEKINDLNPTILDTNPQLYFHLQQQKLIELIRVGKINEALEFAQEELAPRGEENQAFLEEIEKTVALLVFEDVKNCPYGELLDVSQRLKTASEVNAAILTSQSHEKDPKLPSLLKMLIWTQNQLHEKAAYPRISNFSTAALEDPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1HBN9</accession>
    <name>I1HBN9_BRADI</name>
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        <recommendedName>
            <fullName evidence="4">Xylanase inhibitor N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_2g02130v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HBN9"/>
    <dbReference type="EnsemblPlants" id="KQK02538">
        <property type="protein sequence ID" value="KQK02538"/>
        <property type="gene ID" value="BRADI_2g02130v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02538">
        <property type="protein sequence ID" value="KQK02538"/>
        <property type="gene ID" value="BRADI_2g02130v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_807397_0_0_1"/>
    <dbReference type="InParanoid" id="I1HBN9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="TAXi_N"/>
    </dbReference>
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        <property type="entry name" value="TAXi_N"/>
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    </dbReference>
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        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094519" description="Xylanase inhibitor N-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanase inhibitor N-terminal" evidence="4">
        <location>
            <begin position="69"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14543"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK02538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="344" mass="36666" checksum="A51B0A2DE16F4D55" modified="2012-06-13" version="1">MWQRKTTLILVLVLAAVSLRACTGTTSPTGKPLVTVVNIDQSTNLFTSPLNSGRPLVLDLSSAALSTACNTQTTKVTISAFETDGKKLLDRASGVAGLGPSPLSFSSQVASLERLPNKFALCLPGERPFDTGAAIFGGGPLFSPSVITNDESFDSPPTEGQDIAKLLSPEIPLRRLPHTGGLERCQDRPDGTVRAVLLTERAGPGILLAAGGFHAGGRAELDRQPHQVRVPVGRMLRVRQGEAAAERGDAGGGHRRVQHGRQGRGVRPGQAEYQLHSTDQPQDLLRERQIHALRELDCSSWARAFGGASGNGTSAMARIIRPSWQYGMWVPTKCTIGPHLSPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCT9</accession>
    <name>I1HCT9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100828575</name>
        <name evidence="11" type="ORF">BRADI_2g05590v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564624.1">
        <property type="nucleotide sequence ID" value="XM_003564576.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCT9"/>
    <dbReference type="FunCoup" id="I1HCT9">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HCT9"/>
    <dbReference type="EnsemblPlants" id="KQK03098">
        <property type="protein sequence ID" value="KQK03098"/>
        <property type="gene ID" value="BRADI_2g05590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828575"/>
    <dbReference type="Gramene" id="KQK03098">
        <property type="protein sequence ID" value="KQK03098"/>
        <property type="gene ID" value="BRADI_2g05590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828575"/>
    <dbReference type="eggNOG" id="ENOG502QSFP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038424_0_0_1"/>
    <dbReference type="OMA" id="TMPAFYP"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HCT9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:response to ethylene"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009739">
        <property type="term" value="P:response to gibberellin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:response to sucrose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="transcription factor MYB1R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
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        <property type="entry name" value="Myb_dom_plants"/>
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        <property type="entry name" value="Plant_Stress_Dev_TF"/>
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    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44191:SF4">
        <property type="entry name" value="OS01G0187900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44191">
        <property type="entry name" value="TRANSCRIPTION FACTOR KUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="3"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="105"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="105"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="57"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK03098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="368" mass="38833" checksum="A748EC1E33A33F54" modified="2012-06-13" version="1">MTRRCSHCSNNGHNARTCPARGGGGGGGGGVRLFGVHLTSPPVASMKKSASMSCIASSLGGGSGGSSPAAGAGGGGGGARGGGEGAPGYVSDDPMHASCSTNGRAERKKGTPWTEEEHRMFLMGLQKLGKGDWRGISRSFVVSRTPTQVASHAQKYFIRQTNSSRRKRRSSLFDMVPEMPMDESPTGAEEFMVCSTQEESSSSNKLSLFHLGQHKEAEFDKDVPTMHLRQHKESEFAEPSLEAPDLDMNHAVSFKAATVQTMPAFYPTLVPVPLTLWPQNVANVEVAGTTHEILKPTPVNGKEAVKGDEVVSMSKLSIGDGSSGSLEPSALSLQLTGSTNTRQSAFHVNPPMTRTDLSKGNNSPIHAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCU7</accession>
    <name>I1HCU7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100832025</name>
        <name evidence="17" type="ORF">BRADI_2g05620v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>oxaloacetate + H(+) = pyruvate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15641"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="EC" id="1.1.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Photosynthesis; C4 acid pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230567.1">
        <property type="nucleotide sequence ID" value="XM_010232265.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCU7"/>
    <dbReference type="FunCoup" id="I1HCU7">
        <property type="interactions" value="2728"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HCU7"/>
    <dbReference type="EnsemblPlants" id="KQK03102">
        <property type="protein sequence ID" value="KQK03102"/>
        <property type="gene ID" value="BRADI_2g05620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832025"/>
    <dbReference type="Gramene" id="KQK03102">
        <property type="protein sequence ID" value="KQK03102"/>
        <property type="gene ID" value="BRADI_2g05620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832025"/>
    <dbReference type="eggNOG" id="KOG1257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011405_5_2_1"/>
    <dbReference type="OMA" id="EVPVTPW"/>
    <dbReference type="OrthoDB" id="5365701at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HCU7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000002">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000067">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF94">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="16">
        <location>
            <begin position="193"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="15">
        <location>
            <begin position="384"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="62"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="568"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK03102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="668" mass="72966" checksum="05ECC68325A610E3" modified="2012-06-13" version="1">MLSARTAAASPAASLWKRAGGEGGSGNGNGNNGCTSCREVRRRAAVVTVRATPPRRVEAVAMESAAAEIAEAEAEAVAMESAVAEAETAEEEAEKEVAAAGGGVEDKYGEDRATEELPVTPWAFSVASGYTLLRDPHHNKGLAFTEKERDAHYLRGLLPPGVVSQDLQVKKIMHNLRQYKVPLQRYVAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYVSLRDKGKVLEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEQLLNDEFYIGLRQRRATGEEYHELMEEFMGAVKQIYGEKVLIQFEDFANHNAFDLLAKYSQSHLVFNDDIQGTASVVLAGLLSSLKIVGGNLAEHTYLFLGAGEAGTGIAELIALEISKQTDNPIEECRKRIWLADSKGLIVSSRRDSLQPFKKSWAHEHEPVTTLLDAVKSLKPTVLIGTSGVGKAFTKEVIEAMASFNEKPVIFSLSNPTSHSECTAEEAYRWTKGRAVFASGSPFDPVVYDEKTYVPGQANNAYIFPGLGLGVVISGAIRVHEDMLLAASETLAEQATQENFAKGSIFPPFANIRKISALIAASVAAKAYELGLATRLPRPRDLEKYAESCMYTPVYRSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HDS9</accession>
    <name>I1HDS9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100825498</name>
        <name evidence="4" type="ORF">BRADI_2g08480v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314390.1">
        <property type="nucleotide sequence ID" value="XM_024458622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HDS9"/>
    <dbReference type="SMR" id="I1HDS9"/>
    <dbReference type="STRING" id="15368.I1HDS9"/>
    <dbReference type="EnsemblPlants" id="KQK03543">
        <property type="protein sequence ID" value="KQK03543"/>
        <property type="gene ID" value="BRADI_2g08480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825498"/>
    <dbReference type="Gramene" id="KQK03543">
        <property type="protein sequence ID" value="KQK03543"/>
        <property type="gene ID" value="BRADI_2g08480v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S1XJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1117157_0_0_1"/>
    <dbReference type="OMA" id="MNDFRAV"/>
    <dbReference type="OrthoDB" id="913402at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF19">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="19"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK03543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="234" mass="24546" checksum="A7FC6D8C76CCC6F4" modified="2012-06-13" version="1">MTGGLNGNGVAGIGNGGSGACAVCKHQRRKCEPNCELAAYFPANRMNDFRALHLVFGVANLTKLIKANATEEARRRAAETLTWEARWRERDPSEGCYREVSCLRRENAVLRAENAALRRRADQCASCCCGTTVLQQQQQQQQMLLVSAYNGARPPVLHGVSNGSVLPGSFVGGCYNNGGNNNAGGGGRGANGNGAMPPARPPVAQSTTLIGYHAVSNGGAGAGQTDSRDKSGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1HF43</accession>
    <name>I1HF43_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100824069</name>
        <name evidence="6" type="ORF">BRADI_2g12390v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567647.1">
        <property type="nucleotide sequence ID" value="XM_003567599.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HF43"/>
    <dbReference type="EnsemblPlants" id="KQK04216">
        <property type="protein sequence ID" value="KQK04216"/>
        <property type="gene ID" value="BRADI_2g12390v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT70456">
        <property type="protein sequence ID" value="PNT70456"/>
        <property type="gene ID" value="BRADI_2g12390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824069"/>
    <dbReference type="Gramene" id="KQK04216">
        <property type="protein sequence ID" value="KQK04216"/>
        <property type="gene ID" value="BRADI_2g12390v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70456">
        <property type="protein sequence ID" value="PNT70456"/>
        <property type="gene ID" value="BRADI_2g12390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824069"/>
    <dbReference type="eggNOG" id="KOG0765">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_3_3_1"/>
    <dbReference type="OMA" id="VSCVYYV"/>
    <dbReference type="OrthoDB" id="250329at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HF43">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080:SF5">
        <property type="entry name" value="OS01G0329400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="4">
        <location>
            <begin position="32"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="4">
        <location>
            <begin position="130"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="4">
        <location>
            <begin position="237"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK04216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="35622" checksum="3BD0E7E911949CB5" modified="2012-06-13" version="1">MAAATAADTTDASTAGLALAEANINWERLDKTKFHVIGAILFTAQQGVLHPTAVVKTRMQVAEGGLSHMSGFAVFRRILRSDGIPGVFRGFGTTAVGALPGRVLALTSLEVSKEMAFKYSERFDMSEASRIAVANGVAGLVSSIFSSSYFVPLDVICQRLMVQGLPGMQTYRGPLDVINKVVRTEGLRGLYRGFGITMLTQSPASALWWSSYGGAQHAIWRSLGYVNDSQKKPSQSELIAVQATAGTIAGACSSIITTPIDTIKTRLQVMDNYGSGRPSVVKTTRLLLQEDGWKGLYRGFGPRFLNMSLWGTSMIVTYELIKRLSVKPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1HG27</accession>
    <name>I1HG27_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100838773</name>
        <name evidence="14" type="ORF">BRADI_2g15460v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565859.1">
        <property type="nucleotide sequence ID" value="XM_003565811.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HG27"/>
    <dbReference type="FunCoup" id="I1HG27">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK04711">
        <property type="protein sequence ID" value="KQK04711"/>
        <property type="gene ID" value="BRADI_2g15460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838773"/>
    <dbReference type="Gramene" id="KQK04711">
        <property type="protein sequence ID" value="KQK04711"/>
        <property type="gene ID" value="BRADI_2g15460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838773"/>
    <dbReference type="eggNOG" id="KOG2287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051579_0_0_1"/>
    <dbReference type="OMA" id="MREGANC"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000043">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF363">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK04711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="314" mass="35623" checksum="008B5C0120685BD7" modified="2012-06-13" version="1">MLSSPFSHVPFLLVPFLLLALIYLSVSPNDHFSLRSAFAPCNTNSNAPAQRPGAADVDLRVLLGVLTLPSSYERRALLRLAYKLQPPPTGATVDVRFVFCNVAKEEDAVLVAMEIIAYDDILVLNCTENMNDGKTYDFFSAVPKLFADQVPSYDYVGKVDDDIYYRVSPLADALRSKARRDAYYGFEWPCEPETRPGDERFMVGWGYFVSWDVAAWISETEELRGAAKGAEDMTFSRWLRRGGKGKNMYGEKPSRMYDYLNDGWGEKAACFRHNFTADTVAVHKLKDRRQWARALKFFNATQGLKPSKMYHLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1HG42</accession>
    <name>I1HG42_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100829590</name>
        <name evidence="1" type="ORF">BRADI_2g15550v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567836.1">
        <property type="nucleotide sequence ID" value="XM_003567788.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HG42"/>
    <dbReference type="FunCoup" id="I1HG42">
        <property type="interactions" value="1341"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HG42"/>
    <dbReference type="EnsemblPlants" id="KQK04728">
        <property type="protein sequence ID" value="KQK04728"/>
        <property type="gene ID" value="BRADI_2g15550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829590"/>
    <dbReference type="Gramene" id="KQK04728">
        <property type="protein sequence ID" value="KQK04728"/>
        <property type="gene ID" value="BRADI_2g15550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829590"/>
    <dbReference type="eggNOG" id="ENOG502QRBA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068761_0_0_1"/>
    <dbReference type="OMA" id="ELENWNC"/>
    <dbReference type="OrthoDB" id="439326at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35738">
        <property type="entry name" value="OS05G0577800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35738:SF3">
        <property type="entry name" value="OS05G0577800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK04728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="429" mass="47618" checksum="A3D7B3BB318FF676" modified="2012-06-13" version="1">MGILWSSFRSEPPPPPPPMVLVPPLFDYPPIAARTRMSVPAYELMFGKLPLRNLFEDYFDQAGNMTSRIMLKPLEDPHVDLIATVSAAADKNSGTEVKGDALFRWQKDLDNPHTFVDLLVSTSEPMSQLRSCAYYPEYRIGAFGTFPLLMGNRVCSEDYGVMGVRYGSENLSFGASFVPFPESAEVPYGAWLAGRKGSLSAGAQYKPLSGNKHHMPYMDSKNWNCAISYGVGSTSPLSPSFIFSLELARSTQLIASFYRHMVVQRRVKNPFEDGQVVGITNYIDLGLELATTTDKSKPADSANSSMFQLAASWQANKNFLLKGKLGPSKSSIALAFKSWWRPSFTFSVTAVNDHSKGATSYGFGIHVEDLRKASYERADPNYVMLTPNKEHLAPGVLHEYGKRPMFQADVDSGNYDHLPTELKPIDKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HGN6</accession>
    <name>I1HGN6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDP-diacylglycerol--serine O-phosphatidyltransferase</fullName>
            <ecNumber evidence="10">2.7.8.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phosphatidylserine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100833489</name>
        <name evidence="11" type="ORF">BRADI_2g17230v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) is replaced by L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a CDP-1,2-diacyl-sn-glycerol + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="10"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567934.1">
        <property type="nucleotide sequence ID" value="XM_003567886.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HGN6"/>
    <dbReference type="FunCoup" id="I1HGN6">
        <property type="interactions" value="502"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HGN6"/>
    <dbReference type="EnsemblPlants" id="KQK04990">
        <property type="protein sequence ID" value="KQK04990"/>
        <property type="gene ID" value="BRADI_2g17230v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK04993">
        <property type="protein sequence ID" value="KQK04993"/>
        <property type="gene ID" value="BRADI_2g17230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833489"/>
    <dbReference type="Gramene" id="KQK04990">
        <property type="protein sequence ID" value="KQK04990"/>
        <property type="gene ID" value="BRADI_2g17230v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04993">
        <property type="protein sequence ID" value="KQK04993"/>
        <property type="gene ID" value="BRADI_2g17230v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833489"/>
    <dbReference type="OrthoDB" id="10265393at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00615"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HGN6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106245">
        <property type="term" value="F:L-serine-phosphatidylethanolamine phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004277">
        <property type="entry name" value="PSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF20">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03034">
        <property type="entry name" value="PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK04990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="433" mass="50207" checksum="A381FE574ACCCD03" modified="2012-06-13" version="1">MEPPPKQRAARHGDERRRPSLAAAAAADDAVSAAAEEYDPWTAWLYKPRTISVLLVGACLLIWASGALDPEGASSHTSVTSVKRGVWAMIAVFLAYCTLQAPSTILIRPHPAVWRLVHGLAVVYLVALTFLLFQNRDDARQFMKHLYPDLGVELPERSYGADCRLYVPENPKNKFINIYDTLFDEFVVAHVLGWWGKAVMIRNQSLLWVLSIGFELMELTFRHMLPNFNECWWDSIILDILICNWFGIWAGMHTVRYFDGKTYEWVGLSRQPSIMGKVKRSLSQFTPAQWDKDQWQPFMGPLRFVQVLFLCVVFMMVELNTFFLKFCLWIPPRNPLVVYRLILWWLIAIPTIREYNSYLQDRKPVKKVGAFCWLSVAICIVELLICMKFGHGLFHDQMPTWLVIFWSSAGIALVIFLLAWSWRNHQKFRRKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HM91</accession>
    <name>I1HM91_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Embryo defective 2735</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100845186</name>
        <name evidence="2" type="ORF">BRADI_2g37140v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK07703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003568950.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HM91"/>
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        <property type="gene ID" value="BRADI_2g37140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845186"/>
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        <property type="protein sequence ID" value="KQK07703"/>
        <property type="gene ID" value="BRADI_2g37140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845186"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102720_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK07703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="19280" checksum="186DB82D7B9E2A91" modified="2012-06-13" version="1">MKFRVVCRKLYDYVRYDLREIAFPSSLPDPPGYKPRPKLTLNEKWCILKEATRLYAASWVRDIGPELRPNDYKKVKEESSSSKSEEGKTTSEPTVLEDLAVAARGGAETLKPALRRIYMTRASTYTSAVKNYVETYQEGLKDVLDEKVAGKGHQPGSEPTKSSTSPPPPPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2023-06-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>I1HP66</accession>
    <name>I1HP66_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g42855v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HP66"/>
    <dbReference type="EnsemblPlants" id="KQK08612">
        <property type="protein sequence ID" value="KQK08612"/>
        <property type="gene ID" value="BRADI_2g42855v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08612">
        <property type="protein sequence ID" value="KQK08612"/>
        <property type="gene ID" value="BRADI_2g42855v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3053116_0_0_1"/>
    <dbReference type="InParanoid" id="I1HP66"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK08612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="54" mass="6026" checksum="18E34BA7A7CDBDBC" modified="2012-06-13" version="1">MVGENVIVADPQVQWKGQAATEHGETYENLQLGSFQPQVHKPSRSLGEPPGYYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPH8</accession>
    <name>I1HPH8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100821700</name>
        <name evidence="6" type="ORF">BRADI_2g43810v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232036.1">
        <property type="nucleotide sequence ID" value="XM_010233734.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPH8"/>
    <dbReference type="EnsemblPlants" id="KQK08774">
        <property type="protein sequence ID" value="KQK08774"/>
        <property type="gene ID" value="BRADI_2g43810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821700"/>
    <dbReference type="Gramene" id="KQK08774">
        <property type="protein sequence ID" value="KQK08774"/>
        <property type="gene ID" value="BRADI_2g43810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821700"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001724_3_0_1"/>
    <dbReference type="OMA" id="MEHATIS"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000051">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000054">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48046:SF6">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48046">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 72E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK08774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="488" mass="52264" checksum="9906CAB5CFB6609F" modified="2012-06-13" version="1">MAIEAASATPAPERSTTHVVLLACPGAGHVLPVAELARRIVAHNDGFTATLVTHTNFSSGDHYASTFASLPPSVSTAQLPEMSIDDLPADARAETRIITIVKRALPHLRDLLLRLLDSPAGVAAFVPDLFCPWALEVSGELGIPGYIFCPSNLMGLSSLLYTPELDRTTTCEFRDLPDLVRLPGCVPLHGADLVDTIQDRGDPAYPLMVELGEHYLLAQGFLVNTFDAMEHETILAFQELSDKGVYAPAYAVGPFVRSCSNESNKHSSLQWLDAQPDGSVLYVCFGSGGTLSTAQTAELAAGLEASGQRFLWVTQFPSDKDRCASFFGGGRDGDSPVNYLPEGFVERTRGTGLAVPLWAPQVEVLNHPAVAGFVSHCGWNSTLEAVAAGVPTMAWPLYAEQRMNAVMLSERVGLALRPRAREEDGVVPRDEVARVARELMVGEKGAAARKKARELREAAQKGLAPGGKSREALEAIAGKWKKAVGNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUB5</accession>
    <name>I1HUB5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100822213</name>
        <name evidence="4" type="ORF">BRADI_2g57920v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564861.1">
        <property type="nucleotide sequence ID" value="XM_003564813.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUB5"/>
    <dbReference type="FunCoup" id="I1HUB5">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.A08"/>
    <dbReference type="EnsemblPlants" id="KQK11068">
        <property type="protein sequence ID" value="KQK11068"/>
        <property type="gene ID" value="BRADI_2g57920v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822213"/>
    <dbReference type="Gramene" id="KQK11068">
        <property type="protein sequence ID" value="KQK11068"/>
        <property type="gene ID" value="BRADI_2g57920v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822213"/>
    <dbReference type="eggNOG" id="KOG1515">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_14_0_1"/>
    <dbReference type="OMA" id="IEWRDAN"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF211">
        <property type="entry name" value="B1065G12.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="127"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK11068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="402" mass="43326" checksum="820A9A54B8BC6069" modified="2012-06-13" version="1">MPSVGVKIYSVFFKLLLRHRLQSLAAGEGGADAFGVSCRPDEATAPANPAFSAADGVASKDLHIDPNSALSVRVFLPTPPPHAHLLNQRRASEPAAGAAAAPYRGYLPHAVSSPRAAASARRRLPIVVQFHGGGFVTGSNSSASNDAFCRRVAKACDAIVVAVGYRLAPESRYPAAFDDGVRVLKWIAKQANLAMMSKVGGGVDTFGASTVEPWIAAHGDPARCVLLGVSCGANIADFVARKVVEDGKLFNPVKVVAQVLMYPFFIGSVPTHSEIRLANSYFYDKSTCILAWRLLLSEKEFSLDHPAANPLAPGRGGPPLKCMPPTLTIIAEHDWMRDRAIAYSEELRKVNVDAPVLDYKDTVHEFATLDVFLKTPQAQACAEDIAIWMKKYISLRGHEFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUC1</accession>
    <name>I1HUC1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Gamma-butyrobetaine hydroxylase-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100834708</name>
        <name evidence="4" type="ORF">BRADI_2g57960v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567370.1">
        <property type="nucleotide sequence ID" value="XM_003567322.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUC1"/>
    <dbReference type="STRING" id="15368.I1HUC1"/>
    <dbReference type="EnsemblPlants" id="KQK11076">
        <property type="protein sequence ID" value="KQK11076"/>
        <property type="gene ID" value="BRADI_2g57960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834708"/>
    <dbReference type="Gramene" id="KQK11076">
        <property type="protein sequence ID" value="KQK11076"/>
        <property type="gene ID" value="BRADI_2g57960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834708"/>
    <dbReference type="eggNOG" id="ENOG502RZ9H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117841_1_0_1"/>
    <dbReference type="OMA" id="SGLFTWE"/>
    <dbReference type="OrthoDB" id="19707at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35303">
        <property type="entry name" value="OS02G0197800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35303:SF5">
        <property type="entry name" value="OS02G0197800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-butyrobetaine hydroxylase-like N-terminal" evidence="3">
        <location>
            <begin position="39"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK11076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="146" mass="16214" checksum="65789C24353A063B" modified="2012-06-13" version="1">MAAAAAAMMGAAARRLHHAAAAAAGQPPRLKKLALHPPKSVEVEFADGSSFHLSAEFLRVYSPAADSKIRSIAGEKVIFARRHVGIMSAESIGNYGVRILFDDLHKTGIFTWDFLYHLGSNKFSLMRNYIKMLRRHGLTRDPQRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HWH4</accession>
    <name>I1HWH4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100842141</name>
        <name evidence="7" type="ORF">BRADI_3g01837v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Secreted</location>
            <location evidence="5">Extracellular space</location>
            <location evidence="5">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the plant dirigent protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318224.1">
        <property type="nucleotide sequence ID" value="XM_024462456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HWH4"/>
    <dbReference type="EnsemblPlants" id="KQJ92957">
        <property type="protein sequence ID" value="KQJ92957"/>
        <property type="gene ID" value="BRADI_3g01837v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842141"/>
    <dbReference type="Gramene" id="KQJ92957">
        <property type="protein sequence ID" value="KQJ92957"/>
        <property type="gene ID" value="BRADI_3g01837v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842141"/>
    <dbReference type="eggNOG" id="ENOG502S4MA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078923_0_0_1"/>
    <dbReference type="OrthoDB" id="581739at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009699">
        <property type="term" value="P:phenylpropanoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.480.10">
        <property type="entry name" value="Allene oxide cyclase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044859">
        <property type="entry name" value="Allene_oxi_cyc_Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004265">
        <property type="entry name" value="Dirigent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46506">
        <property type="entry name" value="OS05G0143600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03018">
        <property type="entry name" value="Dirigent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00915">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0052">Apoplast</keyword>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="6">
        <location>
            <begin position="165"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ92957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="313" mass="34565" checksum="364C256FDD7F7CDC" modified="2012-06-13" version="1">MSISDFLVTPFGGAVENAEFHLSNLYLHINDTDPDLNEQMIADNAGAGKYGRTYATRWAIHHGVDSAAELVARAPGLITYASDSWHQGFTINFQNQRFKGSTLQVMGVDYDQDEWAIVGGTGEFAMARGIIKKYPHSTARGRVVVKLNIHGFCSMKPTRLGIPYPRKTQTWGGNVGQTLDVIPQPWRLESFKIHEDNGFISSFALKYVDRTGSMYYLGPYWGDVPGTTKITLSPSETLQQVHGTSDNYRGTTIVTSLTFTISNRAADETFGKSSGTDFSPALAQDECIVGFYGQSYGKFLTSIGFYVHKSSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1I302</accession>
    <name>I1I302_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">H/ACA ribonucleoprotein complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Nucleolar protein family A member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100844088</name>
        <name evidence="11" type="ORF">BRADI_3g21230v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573741.1">
        <property type="nucleotide sequence ID" value="XM_003573693.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I302"/>
    <dbReference type="STRING" id="15368.I1I302"/>
    <dbReference type="EnsemblPlants" id="KQJ96137">
        <property type="protein sequence ID" value="KQJ96137"/>
        <property type="gene ID" value="BRADI_3g21230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844088"/>
    <dbReference type="Gramene" id="KQJ96137">
        <property type="protein sequence ID" value="KQJ96137"/>
        <property type="gene ID" value="BRADI_3g21230v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844088"/>
    <dbReference type="eggNOG" id="KOG3387">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084513_4_1_1"/>
    <dbReference type="OMA" id="VRTHANV"/>
    <dbReference type="OrthoDB" id="1924699at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000002">
        <property type="entry name" value="NHP2-like protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="10">
        <location>
            <begin position="21"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ96137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="128" mass="13825" checksum="A314DF16736EF48F" modified="2012-06-13" version="1">MSQEAVNPKAYPLADAQLAQSIQELINQAANYKQLKKGANEATKTLNRGIAEFVVMAADAEPLEILLHLPLLAEDKNVPYVFVPSMQALGRACGVTRPVIACSVTSNEGSNLKDPINKLKIAIEKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1I4Q8</accession>
    <name>I1I4Q8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100829307</name>
        <name evidence="3" type="ORF">BRADI_3g28877v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_024460379.1"/>
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    <dbReference type="AlphaFoldDB" id="I1I4Q8"/>
    <dbReference type="EnsemblPlants" id="PNT67552">
        <property type="protein sequence ID" value="PNT67552"/>
        <property type="gene ID" value="BRADI_3g28877v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829307"/>
    <dbReference type="Gramene" id="PNT67552">
        <property type="protein sequence ID" value="PNT67552"/>
        <property type="gene ID" value="BRADI_3g28877v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1987">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040595_0_0_1"/>
    <dbReference type="OMA" id="FELSIFK"/>
    <dbReference type="OrthoDB" id="1883087at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46162:SF7">
        <property type="entry name" value="MATH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46162">
        <property type="entry name" value="TRAF-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="2">
        <location>
            <begin position="30"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="2">
        <location>
            <begin position="187"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT67552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="321" mass="35922" checksum="D3D507CEEF0D2D88" modified="2012-06-13" version="1">MGASLSRGRLNEGRSQKSKGAGIVPPSPAQTTFKWRINGFSSLLDKDEGLTYSRVFEITGLNWYLKLNPRDRKSGDKNEYVSLKLELARACVRSSTVVEASFKFLIYDQAYGKHQEHLVRHNFQTASTSSGTSCMIPLTTLNKHSSGFLMGDSCVFGVEFIKVATTKANDTSETLFVQKANNTFSDPEVYTWNIEDFFALKSMDNSPEFEIGGHKWSIIIYPSGAANNGNYLSLYLEAKMLDTLHQNSANLVELSICVKDQETGKHRKLTGRCQFSKKSTKWGWDKFISLENFKDSSNGYLVKTKCCIEVEVAIVGSSKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1I501</accession>
    <name>I1I501_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ripening-related protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g29690v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kiwellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I501"/>
    <dbReference type="FunCoup" id="I1I501">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I501"/>
    <dbReference type="EnsemblPlants" id="KQJ97250">
        <property type="protein sequence ID" value="KQJ97250"/>
        <property type="gene ID" value="BRADI_3g29690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97250">
        <property type="protein sequence ID" value="KQJ97250"/>
        <property type="gene ID" value="BRADI_3g29690v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXVH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047639_4_2_1"/>
    <dbReference type="InParanoid" id="I1I501"/>
    <dbReference type="OMA" id="MANTIPI"/>
    <dbReference type="OrthoDB" id="406505at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22270">
        <property type="entry name" value="DPBB_kiwellin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039271">
        <property type="entry name" value="Kiwellin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191:SF71">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24300">
        <property type="entry name" value="KWL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095042" description="Ripening-related protein 4" evidence="6">
        <location>
            <begin position="24"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ97250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="22428" checksum="12D8D1C4CC3A9BC8" modified="2012-06-13" version="1">MASTRLLLLAVFALLQILYSVHGAVEDVGFTSAVNSTRHKPKHPHPHPGGGHGQCRASGVLHGKASKCNKAHGSECCAAGRRYPQFKCSPPVTAKTPATLTVNSFARGGDGGGKSFCDDHFHGDRELVVALSTGWLRLDGTNRCNRKVRVSHGGKSVVAKVVDECDSVNGCDPEHNFEPPCPNNVVDGSPGVWKALGLDDGIGEVKVTWSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1I9M9</accession>
    <name>I1I9M9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100837194</name>
        <name evidence="4" type="ORF">BRADI_3g43320v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574965.1">
        <property type="nucleotide sequence ID" value="XM_003574917.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I9M9"/>
    <dbReference type="FunCoup" id="I1I9M9">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I9M9"/>
    <dbReference type="EnsemblPlants" id="KQJ99455">
        <property type="protein sequence ID" value="KQJ99455"/>
        <property type="gene ID" value="BRADI_3g43320v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99459">
        <property type="protein sequence ID" value="KQJ99459"/>
        <property type="gene ID" value="BRADI_3g43320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837194"/>
    <dbReference type="Gramene" id="KQJ99455">
        <property type="protein sequence ID" value="KQJ99455"/>
        <property type="gene ID" value="BRADI_3g43320v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99459">
        <property type="protein sequence ID" value="KQJ99459"/>
        <property type="gene ID" value="BRADI_3g43320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837194"/>
    <dbReference type="eggNOG" id="KOG1698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076458_1_0_1"/>
    <dbReference type="OMA" id="SSIKQMM"/>
    <dbReference type="OrthoDB" id="432645at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I9M9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.790:FF:000003">
        <property type="entry name" value="50S ribosomal protein L19, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_bL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="Ribosomal_bL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01024">
        <property type="entry name" value="rplS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680:SF17">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00061">
        <property type="entry name" value="RIBOSOMALL19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ99455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24161" checksum="BCF66189DA0BE819" modified="2012-06-13" version="1">MQSLLRTVYRIGSHGTCTRFMDFAASGVAQSSIKQMMPHSWIRPTESPIIHHNGIYVKALAMRGFSTVGNTEVSLEDEISSSTAVEHPPRIKFKRPDKTARHIMNILNKEAVDKVRVERAIPDVQPGCILQMRVQVPENKRRESTLKGIVIARRNAGINTTFRLRRLVAGVGVESVFPLYSPNIKEIKILDRKKVRRAKLYYLRDRMNALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IA16</accession>
    <name>I1IA16_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100834524</name>
        <name evidence="7" type="ORF">BRADI_3g44380v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Probable RNA-binding protein that may play a role in growth regulation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575037.1">
        <property type="nucleotide sequence ID" value="XM_003574989.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IA16"/>
    <dbReference type="EnsemblPlants" id="KQJ99630">
        <property type="protein sequence ID" value="KQJ99630"/>
        <property type="gene ID" value="BRADI_3g44380v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834524"/>
    <dbReference type="Gramene" id="KQJ99630">
        <property type="protein sequence ID" value="KQJ99630"/>
        <property type="gene ID" value="BRADI_3g44380v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834524"/>
    <dbReference type="eggNOG" id="KOG4660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012447_1_0_1"/>
    <dbReference type="OMA" id="NQANVGE"/>
    <dbReference type="OrthoDB" id="417481at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045927">
        <property type="term" value="P:positive regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045836">
        <property type="term" value="P:positive regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12524">
        <property type="entry name" value="RRM1_MEI2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12529">
        <property type="entry name" value="RRM2_MEI2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12531">
        <property type="entry name" value="RRM3_MEI2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000063">
        <property type="entry name" value="MEI2-like protein 5 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000101">
        <property type="entry name" value="Protein MEI2-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034453">
        <property type="entry name" value="MEI2-like_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034454">
        <property type="entry name" value="MEI2-like_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="277"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="362"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="589"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="668"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="744"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="945"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="777"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="962"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00055084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ99630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="987" mass="108847" checksum="2B04FC6710EB67B8" modified="2012-06-13" version="1">MPSQVMDQRHQLSQYRNPIVTASSFSDELLLPTERQIGFWKPESIPHNMGSKSVASSPLEKPQPIGTKIAGRLELIQQYDPKDQKTAYSLEHKPFGQERHANLPPSPWRPQQNPSSQSASSLKATPLLFRDERRTTTNEVYNENGLFSSSLSDIFDKKLRLTTNNALVGKPIQKVDLNHVDDEPFELTEEIEAQIIGNLLPDDDELLSGVLDEVGYAAHTNNGEDVDDDIFYTGGGMELETDESKKLQELTGGANDGFGFLNGALNGEHPHGEHPSRTLFVRNINSNVEDSELKLIFEHYGDIRTLYTACKHRGFVMISYYDIRSARNAMRALQNKPLRRRKLDIHYSIPKDNPSEKDVNQGTLVVFNVDPSVTNDDLRRIFGGYGEIKEIRDTTQKGHHKIIEFYDIRGAESALRALNRNDIAGKKIKLESSHLGGTRRLMQHLSPELGQEEFGVYKLGSPSTSSPSMASFGSSNLATLTSTGFENGSMGMLSGIQTSMSSFRDASFPGLSSTIPQSLSSPVGITSGVNKATLGELSHSLGRMNGHMNYGFQGMGGLTNGSPYNTMTPIGVDSNSRVAEAVDSRHLHKVGSGNHNGHPFDRAEGAPGFSRSGSLPLRGQHLMWNNSNNFHHHPNSPVLWPNPNPASFVNNVPSRPPAQMHGLPRAPAHMLENAPPMHHHHVGSAPAINPSVWDRRHGYAGDLTEAQSFHPGSVGSIGFPGSPQLHSVELNNIFSPNGGSCMDPAVSPAQIGAPSPQQRGMFHGRNPMVPHPLFDSPGERMRNRRNDTSANQSDNKRQYELDVDCILRGEDSRTTLMIKNIPNKYTSKMLLTAIDENHKGTYDFIYLPIDFKNKCNVGYAFINMTNPQHIVPFYQTFNGKKWEKFNSEKVASLAYARIQGKLALIAHFQNSSLMNEDKRCRPILFHSNGPNAGDQEPFPMGANIRARSGRARTSSGEENHQEISSTLASCDTSSNGADTSGPTKDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBB8</accession>
    <name>I1IBB8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100837712</name>
        <name evidence="2" type="ORF">BRADI_3g48036v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylethanolamine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575305.1">
        <property type="nucleotide sequence ID" value="XM_003575257.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBB8"/>
    <dbReference type="STRING" id="15368.I1IBB8"/>
    <dbReference type="EnsemblPlants" id="KQK00226">
        <property type="protein sequence ID" value="KQK00226"/>
        <property type="gene ID" value="BRADI_3g48036v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837712"/>
    <dbReference type="Gramene" id="KQK00226">
        <property type="protein sequence ID" value="KQK00226"/>
        <property type="gene ID" value="BRADI_3g48036v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837712"/>
    <dbReference type="eggNOG" id="KOG3346">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043994_6_1_1"/>
    <dbReference type="OMA" id="SFNFEMF"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000028">
        <property type="term" value="P:regulation of photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.280.10:FF:000001">
        <property type="entry name" value="Terminal flower 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001858">
        <property type="entry name" value="Phosphatidylethanolamine-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF88">
        <property type="entry name" value="FLOWERING LOCUS T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01220">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK00226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="174" mass="19591" checksum="9E1A65999748A19F" modified="2012-06-13" version="1">MSRDPLVVGNVVGDILDPFIKSASLKILYNNRELTNGSDLKPSQVVNEPRIEIAGRDMRNLYTLVMVDPDSPSPSNPTKREYLHWLVTDIPESANASYGNEIVSYESPKPTAGIHRFVFVIFRQSVRQTIDAPGWRPNFNSRDFSALYNLGPPVASVFFNCQRETGCGGRRYIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBS1</accession>
    <name>I1IBS1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100842265</name>
        <name evidence="8" type="ORF">BRADI_3g49310v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575406.1">
        <property type="nucleotide sequence ID" value="XM_003575358.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBS1"/>
    <dbReference type="EnsemblPlants" id="KQK00421">
        <property type="protein sequence ID" value="KQK00421"/>
        <property type="gene ID" value="BRADI_3g49310v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00422">
        <property type="protein sequence ID" value="KQK00422"/>
        <property type="gene ID" value="BRADI_3g49310v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842265"/>
    <dbReference type="Gramene" id="KQK00421">
        <property type="protein sequence ID" value="KQK00421"/>
        <property type="gene ID" value="BRADI_3g49310v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00422">
        <property type="protein sequence ID" value="KQK00422"/>
        <property type="gene ID" value="BRADI_3g49310v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842265"/>
    <dbReference type="eggNOG" id="KOG1441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048347_0_1_1"/>
    <dbReference type="OMA" id="MQDKESH"/>
    <dbReference type="OrthoDB" id="5547497at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IBS1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005464">
        <property type="term" value="F:UDP-xylose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="19"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK00421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="337" mass="36344" checksum="7430DC802E7B1561" modified="2012-06-13" version="1">MTAGFQLGVIGSLTLSVASSVAIVICNKALISTLGFPFATTLTSWHLMVTFCTLHVAQRMRFFEPKAIDGHTVILFGLLNGTSIGLLNLSLGFNSIGFYQMTKLAIIPFTVLLETIFLKKRFSESIKFSLLVLLLGVAIASVTDLKLNLLGSVLSGLAIATTCVGQILTNTIQKKLKVTSTQLLYQSAPYQAAILFATGPFVDQLLTNRSVFAHKYSAPVVGFIVMSCLIAVSVNFSTFLVIGTTSPVTYQVLGHLKTCLVLSFGYTLLHDPFTMRNILGILVAIFGMALYSCFSVMESKRKSAGDALPVLSQMPEKETEPLLETKDSGDTKKANGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICE7</accession>
    <name>I1ICE7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100842464</name>
        <name evidence="16" type="ORF">BRADI_3g51110v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572797.1">
        <property type="nucleotide sequence ID" value="XM_003572749.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICE7"/>
    <dbReference type="FunCoup" id="I1ICE7">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00683">
        <property type="protein sequence ID" value="KQK00683"/>
        <property type="gene ID" value="BRADI_3g51110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842464"/>
    <dbReference type="Gramene" id="KQK00683">
        <property type="protein sequence ID" value="KQK00683"/>
        <property type="gene ID" value="BRADI_3g51110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842464"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_18_3_1"/>
    <dbReference type="OMA" id="ANNGIGH"/>
    <dbReference type="OrthoDB" id="1060944at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ICE7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001678">
        <property type="entry name" value="Calmodulin-binding receptor kinase CaMRLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000649">
        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF55">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095254" evidence="13">
        <location>
            <begin position="28"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="977"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="36"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="380"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK00683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1037" mass="113701" checksum="AF363AFF864AA3C0" modified="2012-06-13" version="1">MASTSMHPAFARFLLILVATLSRAAHALPVAASCLPEERDALLAFKDGISSDPGGVVASWQRGGQEDCCRWRGIRCSNNTGHVLALRLRNVPPGPELDDRGYYAGTALVGRISPSLLSLSRLRHLDLSRNYLEGSPDAAGCALPAFLGGLRSLRYLNLSGIYFSGEVPPQIGNLSRLHTLDLSSDFDARLMRSSDLSWLERLPLLQHLSLSSVDLSRARDWHRAVNMLPALRTLRLSSCSLPASVHQSNPPLLFRNFTNLEELDLSMNQLEHPAAPSWFWNLTSLTSLNLMGTLLYGQLPDSLDAMVSLEILDFSYNGNMATMPRSLKNLCNLRYLDLDSSLADGVDIGEMLESLPQRCSSSRLQELYLPNNGMSGNLPDYRRLMHLTGLRVLDLSYNNITGYIPPSLGNLTTLATLDISSNNLTGLIPTGQGYFPSLSTLVLSSNYLTGDIPAEIGFLASLITLDLGDNYLTGPVPSQISMLSNLTYLDLSLNALVAVVTEEHLASFVNLKKLDLSQNLLVKVEVNSKWKPPFSLHEASFASCFMGPLFPGWLQWQVELFYLDISSTGINDRLPDWFSSTFSKVVDLDISNNSLYGELPGNMEAMSLVEAYLSLNKLTGHVPRLPRNITVLDISMNSLSGPLPSLGASRLRVLILFSNRIVGHLPVSICEARSLAILDLANNLLMGELPSCSAMEGVRYLLLSNNSFSGTFPPFVQSCTSLGFLDLAWNSLTGTLPMWIGNLMQLQFLRLSHNMFTGKIPIVITKLKLLHHLNLAGNDISGSIPRGLSNLTAMTQKAGKVGSFPYQGYADVVGEYGNSLSAVTKGQDLNYGVGILQMVSIDLSFNSLTGIIPEEIAFLDALLNINLSWNHLSGKIPDNIGAIKSLESLDLSKNMLSGEIPSSLSSITYLSFLNLSQNNLTGRIPPGSQLDTLYQEHPSIYDGNSGLCGPPLQKICLTNATTKQDGQKRSKHGFEPMSFYFGLGLGLMLGLWLVFCILLFKKAWRIAYFRLFDKLYDQIYVLVVVKWNSLTRAGTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICL6</accession>
    <name>I1ICL6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_3g51700v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICL6"/>
    <dbReference type="FunCoup" id="I1ICL6">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1ICL6"/>
    <dbReference type="EnsemblPlants" id="KQK00770">
        <property type="protein sequence ID" value="KQK00770"/>
        <property type="gene ID" value="BRADI_3g51700v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00770">
        <property type="protein sequence ID" value="KQK00770"/>
        <property type="gene ID" value="BRADI_3g51700v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031591_5_0_1"/>
    <dbReference type="InParanoid" id="I1ICL6"/>
    <dbReference type="OMA" id="KRDNKDH"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044785">
        <property type="entry name" value="RopGAP1-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23177">
        <property type="entry name" value="MKIAA1688 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23177:SF74">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="139"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="300"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="310"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK00770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="442" mass="48774" checksum="477C32FC1A553B7F" modified="2012-06-13" version="1">MQPSPIRFGRQIPLPCSDSEEEEEEEEEEQYEWEEEAEGEAPVVSPFLLPAARGVVSVVDMVAAVLRRSLLLCSSVRADEEQRAAAARMQIGRPTDVCHVSHVTFDRFVGFLGLPPDLEPEVPCPAPSASISIFGVLRTSMQCSFDKRGNSVPAILLTMQRKLYLLGGLQAEGVFRINADNSQEQHVREQLNRGVVPDGVDLHCLAGLIKAWFRELPSGVLDSLTPEQVMHCNTEEECALVASTVPPVEAALLDWAINLMADVMADPLTALIHAVQVMNFLKTLILKTVKEREEAAAAARAFTSNSGSPSDKDEPQTLDHLDKPFTYSRQQTDERPMISGSTLDHFLFRVEQELHHDAHPSIGEPKCDTGSAHDKSNNKFSPLNSAFSIHDNNSRNKFSGDSVEGLFDMFKFRNGVGRLCRHPVFQLSRSVKKSDEAGQACA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1ID34</accession>
    <name>I1ID34_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100840231</name>
        <name evidence="2" type="ORF">BRADI_3g52990v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572865.1">
        <property type="nucleotide sequence ID" value="XM_003572817.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ID34"/>
    <dbReference type="EnsemblPlants" id="PNT69296">
        <property type="protein sequence ID" value="PNT69296"/>
        <property type="gene ID" value="BRADI_3g52990v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840231"/>
    <dbReference type="Gramene" id="PNT69296">
        <property type="protein sequence ID" value="PNT69296"/>
        <property type="gene ID" value="BRADI_3g52990v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840231"/>
    <dbReference type="eggNOG" id="ENOG502S3KX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1613725_0_0_1"/>
    <dbReference type="OMA" id="FWGRPEQ"/>
    <dbReference type="OrthoDB" id="669276at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ID34">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33264">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33264:SF27">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT69296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="187" mass="20826" checksum="ECA9652EA6565B33" modified="2012-06-13" version="1">MSSSSSSCAAETGLVAMDCLVVCCCCPCLVLQITVFLFVRLPRKVVVKSKRIILRRWRHRHRRRAGAKTRGLGTVSSDGNGMKHLEELLDGDFGGPFGINGTECSDSDSDGWKEGCFAMDGDGRWKQRCFAVDNSYHDGVWEALVEQEGLFWFGSFWGRTEQGDPVSAEDQMYAGLRLPVVLERVHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1IF22</accession>
    <name>I1IF22_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106866514</name>
        <name evidence="9" type="ORF">BRADI_3g58740v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756324.1">
        <property type="nucleotide sequence ID" value="XM_014900838.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IF22"/>
    <dbReference type="EnsemblPlants" id="KQK01838">
        <property type="protein sequence ID" value="KQK01838"/>
        <property type="gene ID" value="BRADI_3g58740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106866514"/>
    <dbReference type="Gramene" id="KQK01838">
        <property type="protein sequence ID" value="KQK01838"/>
        <property type="gene ID" value="BRADI_3g58740v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:106866514"/>
    <dbReference type="eggNOG" id="KOG2640">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051582_0_0_1"/>
    <dbReference type="OMA" id="MIHRNTH"/>
    <dbReference type="OrthoDB" id="19690at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd02999">
        <property type="entry name" value="PDI_a_ERp44_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044606">
        <property type="entry name" value="APRL4/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46854">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE-LIKE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46854:SF1">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE-LIKE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095259" description="Thioredoxin domain-containing protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="30"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK01838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="322" mass="35950" checksum="5A64B801B04A4CFC" modified="2012-06-13" version="1">MARRSPCWPALLLLLLHISPAAVGHSSAASAPPDACPVPTAVESILGPSYTCSPPDRRLGDPVGVIEGDEATLARAINLLHMNKDDYIAILFYASWCPFSQECKPNFEALAHLFPTIRHFAFEESAIRPSIISRYGIHGFPTLFLLNSTMRVRYHGPRTVKPLAAFYSDVSGINASVKSTAEEAMVHSLEDIEPKKDVEQENCPFWWARSPENILQQDTYLALATSFVILRLLYLLFPKIVSLAKQAWRRHTLFGNLMGVHEYFFTYLDQARQKFNRLYPSKRGNLQEGARNATAWASKSLASVSIGEPSTIGRTNSTNELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1IGT2</accession>
    <name>I1IGT2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100828536</name>
        <name evidence="9" type="ORF">BRADI_4g02710v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577678.1">
        <property type="nucleotide sequence ID" value="XM_003577630.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IGT2"/>
    <dbReference type="STRING" id="15368.I1IGT2"/>
    <dbReference type="EnsemblPlants" id="KQJ85978">
        <property type="protein sequence ID" value="KQJ85978"/>
        <property type="gene ID" value="BRADI_4g02710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828536"/>
    <dbReference type="Gramene" id="KQJ85978">
        <property type="protein sequence ID" value="KQJ85978"/>
        <property type="gene ID" value="BRADI_4g02710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828536"/>
    <dbReference type="eggNOG" id="KOG4344">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007396_0_0_1"/>
    <dbReference type="OMA" id="TREVIHE"/>
    <dbReference type="OrthoDB" id="66546at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IGT2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1900:FF:000008">
        <property type="entry name" value="Gamma-tubulin complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF33">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="49"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="706"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ85978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1035" mass="116062" checksum="0C0B618448E75648" modified="2012-06-13" version="1">MVLPQQTGGLLEADVTESFIHKLQLSVSNGLPHAAPVPASRAEEDELVKSVFQVLQGFDTLLLYWDENVPAYCEKAGTYVSHLSRASLGSVLKPLLFAATCLKLVELFVGKVRSCGHGTPTLNAFASSVHSWLMRLREAALKEEEELFLSVDRTITLLGLTDSMSSLCSGAEHLYQVVHGAVPDGFWNSGANIASSEVAVHVLNHIFKKLNEVCLVEDGEGEPYHMLLVIFAGSLLPYLHCLDSWLYDGILDDPYEEMFFYANNAVTVDQPSFWEMSYMLRVRGSRADNSSTLTDTESIRTKESNKQEPANTGACLKASNQGYVDILCPVFLKDIARAIVSAGKSFQLVQHVQSTHHTRTHEGTNGFDVDQHSNHSSRLNRPDILSFEIKAGHLTCEDDLRKSTGQFGHDAREMGLLTLSEIFLICLSGLLENGDHVYEYLRNLPAGSTPDVKALLECKSDAQGTKEACAENSSEKTWLKLLRDAISGRKCDDMEKTLSKSAAMRDPTSVHEYLQDLSSNAVDRHFSPCCYENPAITTCGDALRRNPNSWSNLNISKCFDLPPLNDENMRRAIFGDHHSAGTSICGDTQSTTSFPRLDGTDYKFGFQFDDLEYVRQEDDRRTLEDLYAFPTLLPCVNENVPLSEILPLQKDSTLASRVLKFIQSMALKDPLHPVGIIQECLSKCIKRQVDHIGRQILCKLMGEWRLMDELLVLRAIYLLGSGDMLQQFLITIFDKLDKGNSWDDDFELNTLLQESIRYSADKMLLTAPDSLVVSLAKPDPRYDEESALTSRKGRAQGFGIDALDVLNFTYKVSWPLDLIANAEALKKYNQVMGFLLKVKRAKFVLDETRKWMWKGRGSTAHNFKQHLIVGQKLLHFVDAFHQYVMDRVYHSAWTELCDGMASATTLDEVMEVHEAYLSSIQRQCFVASDKLWALIASRVKTILGLALDFHNVEQTLGTGGTTPAVRSRCEMELDRIDKQFDECVVFLLRILSFKLNVGHFPHLADLVTRINYNHYYMSDTGTFSAIPGSRSRQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMP2</accession>
    <name>I1IMP2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcriptional corepressor SEUSS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100844409</name>
        <name evidence="2" type="ORF">BRADI_4g22770v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ89011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_4g22770v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_4g22770v3"/>
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    </dbReference>
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        <property type="component" value="Chromosome 4"/>
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        <property type="expression patterns" value="baseline and differential"/>
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    </dbReference>
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        <property type="entry name" value="LIM-bd/SEUSS"/>
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    </dbReference>
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        </location>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="609"/>
            <end position="791"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
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        <location>
            <begin position="645"/>
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    </feature>
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        <location>
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        <location>
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            <end position="741"/>
        </location>
    </feature>
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            <end position="769"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ89011.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ89011"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1IR35</accession>
    <name>I1IR35_BRADI</name>
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        <recommendedName>
            <fullName evidence="2">25S rRNA (uridine-N(3))-methyltransferase BMT5-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">LOC100822578</name>
        <name evidence="3" type="ORF">BRADI_4g33200v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010241097.3"/>
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        <property type="gene ID" value="BRADI_4g33200v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822578"/>
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        <property type="gene ID" value="BRADI_4g33200v3"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BMT5-like"/>
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        <property type="entry name" value="25S RRNA (URIDINE-N(3))-METHYLTRANSFERASE BMT5-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
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            <begin position="135"/>
            <end position="300"/>
        </location>
    </feature>
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    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF10354"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ90674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="531" mass="58139" checksum="99E65F323FF28B11" modified="2012-06-13" version="1">MRRPRGPAAAGGKGREKAPPGSAVVVGVKDADGVPAVIAVDGKGGAPVQGVLFVAESGCGEKAPLDAGTAVGGKDAEGVAVIAVGVKDGASAQGDPVVPAVDREVDKPMGKGGVAEKDEEGIKWLKHYSSMQSILIVGDGDFSFSLSLATAFGSGQNLVATSLDTYEDLTKKYVKAESNVTELKSLGAAVLHGVDAKEMKLHPFLKMRRFDRIVFNFPHAGFDGKEDDLHMINKHKQLVNGFFCNARHLLRPYGETHLSHKTGLPYDAWDIEQLAYQSCFTMVEKVDFCKQDYPGYNQKRGDKAKCDQPFALGPCCTFKFCIGDVKKLKKVRANRIDSIPSLGGSGSYPGILVTDMRPSDLHPPAPAWPRPHFPPVNALHIPIAFEPYHLGVNQMEHPGFPVNFYGTDRAPHFNSWGMVRPVCSIPGSPQNVLLTPGGIPPPMRIPNTTLIAPPEHPWYQEGPPVELLRRGGYSFPEREYQRSLQREYEIHRQLMPGGSSLGYSVFLEHRYMESVQRRERLEMLINFYGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1IS29</accession>
    <name>I1IS29_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100846153</name>
        <name evidence="3" type="ORF">BRADI_4g35850v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578486.1">
        <property type="nucleotide sequence ID" value="XM_003578438.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IS29"/>
    <dbReference type="STRING" id="15368.I1IS29"/>
    <dbReference type="EnsemblPlants" id="KQJ91141">
        <property type="protein sequence ID" value="KQJ91141"/>
        <property type="gene ID" value="BRADI_4g35850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846153"/>
    <dbReference type="Gramene" id="KQJ91141">
        <property type="protein sequence ID" value="KQJ91141"/>
        <property type="gene ID" value="BRADI_4g35850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846153"/>
    <dbReference type="eggNOG" id="ENOG502QRMN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076751_0_0_1"/>
    <dbReference type="OMA" id="AECQREI"/>
    <dbReference type="OrthoDB" id="198474at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36383">
        <property type="entry name" value="OS09G0529350 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36383:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ91141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="298" mass="32849" checksum="551119CB8A2B2A33" modified="2012-06-13" version="1">MQHLALLCPLVQSPLLPASPLAARCRRRVRGLGIRGRYAPGEGAGDGVSSAEWLSSAVGEKVDELLQREENRALLEGVEAAERRVELARAALQDIERQEAAARLAREEVRRLEKRRDEIQESQRELLLAREMITEAQRTLSSSLEDQSFGDVSSGDIDEDSERLESVKAAALSSIVGVLASLPISFYEVDSFPRLFVRSLIVFISSALFGVTFRYAIRRDVDNIQLKTGVAAAFAFVRGLAMLESGRPFELSSEAVISLALDGAFSVAESIFIFLPAAIALDFCFKMRFLSPFPTRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1IU78</accession>
    <name>I1IU78_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription repressor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ovate family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100825141</name>
        <name evidence="9" type="ORF">BRADI_4g42080v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional repressor that regulates multiple aspects of plant growth and development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577023.1">
        <property type="nucleotide sequence ID" value="XM_003576975.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IU78"/>
    <dbReference type="FunCoup" id="I1IU78">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IU78"/>
    <dbReference type="EnsemblPlants" id="KQJ92176">
        <property type="protein sequence ID" value="KQJ92176"/>
        <property type="gene ID" value="BRADI_4g42080v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825141"/>
    <dbReference type="Gramene" id="KQJ92176">
        <property type="protein sequence ID" value="KQJ92176"/>
        <property type="gene ID" value="BRADI_4g42080v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825141"/>
    <dbReference type="eggNOG" id="ENOG502RRRQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061898_1_0_1"/>
    <dbReference type="OMA" id="PVVDTGC"/>
    <dbReference type="OrthoDB" id="1928390at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038933">
        <property type="entry name" value="Ovate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006458">
        <property type="entry name" value="Ovate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01568">
        <property type="entry name" value="A_thal_3678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057:SF163">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33057">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR OFP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04844">
        <property type="entry name" value="Ovate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51754">
        <property type="entry name" value="OVATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OVATE" evidence="8">
        <location>
            <begin position="159"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="104"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ92176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="251" mass="26693" checksum="EFA48EDC13CFA3DE" modified="2012-06-13" version="1">MSTSRLRRGSGGRQFAVGGASVSVGGAGRWRHVAVVDGVGCGCRPRRPSASRLLMSLPSFLRPSPKPPAAAPAPRRNASFSSTHSSSASSSFFFPASGAASTTSSSSAYSHDMTAKAATAPAPAPRKKRANNSKAKKRREEKLTAEAEEGEEGDVGVAVEKESSDPRGDFRDSMVQMVVEMGLCDWDGLRCMLRRLLALNAPRHHAAILAAFAEVCAHLASSSSSAPPPPPSPPPPAYQYQYHHYDDYYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1IV91</accession>
    <name>I1IV91_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="7">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100825545</name>
        <name evidence="10" type="ORF">BRADI_4g45065v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="7"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238829.1">
        <property type="nucleotide sequence ID" value="XM_010240527.3"/>
    </dbReference>
    <dbReference type="SMR" id="I1IV91"/>
    <dbReference type="STRING" id="15368.I1IV91"/>
    <dbReference type="EnsemblPlants" id="KQJ92654">
        <property type="protein sequence ID" value="KQJ92654"/>
        <property type="gene ID" value="BRADI_4g45065v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825545"/>
    <dbReference type="Gramene" id="KQJ92654">
        <property type="protein sequence ID" value="KQJ92654"/>
        <property type="gene ID" value="BRADI_4g45065v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825545"/>
    <dbReference type="eggNOG" id="KOG0260">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2992">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002449_2_0_1"/>
    <dbReference type="OMA" id="WGSQQKS"/>
    <dbReference type="OrthoDB" id="1926397at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000418">
        <property type="term" value="C:RNA polymerase IV complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02737">
        <property type="entry name" value="RNAP_IV_NRPD1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040402">
        <property type="entry name" value="NRPD1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF51">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE V SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11523">
        <property type="entry name" value="DUF3223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="9">
        <location>
            <begin position="210"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1302"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1353"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1578"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1869"/>
            <end position="1954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1314"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1405"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1424"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1445"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1493"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1519"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1601"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1650"/>
            <end position="1671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1697"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1735"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1879"/>
            <end position="1892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1917"/>
            <end position="1927"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ92654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1954" mass="215477" checksum="239EF33D9E17706B" modified="2012-06-13" version="1">MEEDQSAVLVAEGAIKSIKLSLSTEDEILTYSINDCPVTHPSQLGNPFLGLPLETGKCESCGASENGKCEGHFGYIELPVPIYHPCHVSELRQLLSLVCLKCLRIKKGKAKQSNGKENVSVTACSYCRDVPALSLKEVKTADGAFRLELRAPPRRLMKDSSWNFLDKYGFHHGGASHFRTLLPEEALNILKKIPDDTRKKLAARGYIAQSGYVMKYLPVPPNCLYIPEFTDGQSIMSYDISISLLKKILHRIEQIKKSRAGTPNFESHEAESSDLQISIAQYIHLRGTTKGPQDTKRFTISTDSSHLSTKQWLEKMRTLFISKGSGFSSRSVLTGDPYIGVDVVGLPSEVAKRITFEEQVTDINIKRLQEVVDKGLCLTYRDGQTTYAITVGSKGYTTLKVGQTISRRIVDGDVVFLNRPPSTHKHSLQAFYVYIHDDHTVKINPLICSPLAADFDGDCVHIYYPQSLAAKAEALELFSVEKQLTNSHNGKVNLQLSNDSLLALKHMSSRTVLSKESANQLAMLLSFSLPDPAVVKLKPCWTITQIIQGALPAALTCEGGRFLVKDSTVIKLDLAKESVQASFSDLVSSILCVKGPGGALQFLNALQPLLMEYLLLDGFSVSLQDFNVPKVLLEEVHKSIQEQSLVLEQSRCSKSQFVEMRVDNNLKDVKQQISDFVVESSHLGLLIDPKSEPSMSKVVQQLGFVGLQLYREGKFYSSRLVEDCFSSFVDKHPPIVGNQHPPEAYGLVQNSYFHGLNPYEELVHSISTREAIVRSSRGLTEPGTLFKNLMAILRDVVICYDGTVRNICSNSIMQLKYNEDDATDIPSALTPGEPVGVLAATAISNPAYKAVLDASQSNNTSWASMKEILQTKVSYKNDTNDRKVILFLNDCSCPKKFCKEKAAIAVQNRLKRVTLEDCATDICIEYHKQILDGSSEATPALVGHIHLEKARLDMINVSTEDILQKCQEVSLKHGKKKGHLGHLFKKITFSTCDCSFTQKPMIDGKLPKVPCLQFSFSEDIPMLSESVERAVSVLANSLCDVLLDTIIKGDPRIQEAKIMWVGSDAQSWVKNTRKVSKGEPTVEIVVEKNEASKQGDAWRIAMDACIPVIDLIDTRRSIPYGIQQVRELLGISCSFDQIVQRLSTTMKTVAKGILKDHLILVANSMTCTGNLYGFNTGGYRATFRALKVQVPFTESTLFTPMKCFEKAAEKCHSDALGCVVSSCSWGKHAALGTGSSFQILWNENQLKSNKEYGDGLYDFLAMVRTDQEKARYTFLDDVDYLVEDNAMDDICLSPELNGTHGVPTFEDNFEHQDTQNGNSWENGTKANASWEQNASAGNDSDNWGGWSNAAAAADTGAAKPADQGNSSWDVPATAENDSTDWGGWGNEKAKDNRTVSTEPAELDTWSDRGAKKGTDGGGGSWGKQTNTCEDSGTNLERNSWAKRPSSPSLSTWAKKNSDGGDGTWDKQANSCKKNVEQDSWKNMPVSPARNAWNKKESSRGDATWEMRASTLEEKKTSESNEGSWEKSNAQKDSWGNTQHGSSDKMAVKDNDMQQDPWGHIATQNINAQDDLWGSVAAKAQTSTAENTDAQDDSWGAVAAKAQTSTAQESWGNVAASPSDNAWKAPPISQTSAAEHTDAHNDSWGIVAAKAQTSTAQQESWGNATASPSDNAWNAAPMDLDAKQPGSWDGWSSALAEDSNKADDSSNKNKGWKSDGWGAKGNRRDQRDNPSMPPMRPDERPPRPRFEVPAEAKKILREIEPIVSMVRKIFRESCDGVRLPLEDEKFIKESILEHHPEKERKVPGEIDHIMVNKHHIFQESRCFYVVLADGTHTDFSYNKCMDNYVRKTYTDAAEHADLVSQMYFKKRDRDRAAAVDGGSTPANASQSTQVMETSQDEAPQEAQPETCVATQEETRVSPQETPAATTQQEETENNPDSASEADYHSASEAGLPEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1IX42</accession>
    <name>I1IX42_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100837743</name>
        <name evidence="8" type="ORF">BRADI_5g08190v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579618.1">
        <property type="nucleotide sequence ID" value="XM_003579570.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IX42"/>
    <dbReference type="FunCoup" id="I1IX42">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IX42"/>
    <dbReference type="EnsemblPlants" id="KQJ82281">
        <property type="protein sequence ID" value="KQJ82281"/>
        <property type="gene ID" value="BRADI_5g08190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837743"/>
    <dbReference type="Gramene" id="KQJ82281">
        <property type="protein sequence ID" value="KQJ82281"/>
        <property type="gene ID" value="BRADI_5g08190v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837743"/>
    <dbReference type="eggNOG" id="ENOG502QRB8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021715_1_0_1"/>
    <dbReference type="OMA" id="GTMQQRY"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IX42">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17354">
        <property type="entry name" value="MFS_Mch1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056555">
        <property type="entry name" value="NFD4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF96">
        <property type="entry name" value="OS04G0388700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23262">
        <property type="entry name" value="NFD4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="556"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Nodulin-like" evidence="6">
        <location>
            <begin position="15"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="NFD4 C-terminal" evidence="7">
        <location>
            <begin position="359"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ82281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="594" mass="64646" checksum="A07A01E6F3D5BD34" modified="2012-06-13" version="1">MGNLAERLRAFSTNRWLVFVAAMWLQSMAGIGYLFGAISPVVKAALGYNQRQVAALGIAKDLGDCVGFLAGTLSAMLPAWAMLLIGAVQNFLGYGWLWLIVTKQAPALPLSMMCVLIFVGTNGETYFNTTSLVTCIQNFPKSRGPTVGILKGFAGLSSAILTQLYAVLHTPDHATLVFMVAVGPSLVALGLMFVIRPVGGHRQVRPSDKNSFMFIYSICLLLASYLVGVMLVQDFLNLSDNVVTSLTVILFILLISPIAIPVTLSFFSKTEYPSPTEEALLSEALKGEASTSQEKEDQPELILSEVEEEKSKEIDSLPPSERRRRIADLQAKLVQAAARGGVRIRSRPHRGENFTLMQALVKADFWLIWLSLLLGSGSGLTVIDNLGQMSQAAGFQDAHNFVSLTSIWNFLGRVGGGYFSEIIVRERAYPRHIALALAQILMAAGHFLFAMAWPGTMYMGTFLVGLGYGAHWAIVPAAVSELFGVKHFGAMYNFLTVANPTGSLIFSGLIASNFYDYEAEKQAHRHQSSVLPSPRLLHGLGLLSDGPLKCEGAVCFVVSSLIMSVFCVMGAGLSLLIVHRTKRVYSRLYRSSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1J148</accession>
    <name>I1J148_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peroxidase</fullName>
            <ecNumber evidence="3">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC104581319</name>
        <name evidence="20" type="ORF">BRADI_5g19857v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">Bd21</strain>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="KQJ84288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="I1J148"/>
    <dbReference type="EnsemblPlants" id="KQJ84288">
        <property type="protein sequence ID" value="KQJ84288"/>
        <property type="gene ID" value="BRADI_5g19857v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581319"/>
    <dbReference type="Gramene" id="KQJ84288">
        <property type="protein sequence ID" value="KQJ84288"/>
        <property type="gene ID" value="BRADI_5g19857v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ2M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010543_0_1_1"/>
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    <dbReference type="OrthoDB" id="2113341at2759"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="F:heme binding"/>
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        <property type="term" value="P:hydrogen peroxide catabolic process"/>
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        <property type="entry name" value="Haem_peroxidase_sf"/>
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    </dbReference>
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        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF254">
        <property type="entry name" value="PLANT HEME PEROXIDASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="9 16" id="KW-0408">Iron</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="19">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
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    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="11"/>
        </location>
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    <feature type="binding site" evidence="16">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="15">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
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        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <location>
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        <ligand>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>2</label>
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            <position position="7"/>
        </location>
    </feature>
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        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
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        </location>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
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        </source>
    </evidence>
    <sequence length="267" mass="28533" checksum="2638C72733DB3820" modified="2012-06-13" version="1">MGASLLRLHFHDCFVQGCDGSILLDDVGSFVGEKTAGPNKNSVRGYDVIDRIKQTLEQMCPGVVSCADIVALAARDSTFLLGGPTWEVLLGRRDSTTTSLADANTDLPAPTSNLDVLISAFAKKNLSPRDLTALSGAHTVGFSQCSNFRDHIYNDTNIDTAFAALRKTDCPAAAPAGNTNLSPLDVETQADVFDNAYYRNLVARRGLLHSDQELFNGASQDALVRQYGNNPALFASDFVTAMIKMGSISPLTGATGEIRLNCRVVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EL23</accession>
    <name>A0A0Q3EL23_BRADI</name>
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        <recommendedName>
            <fullName evidence="1">Exportin-1/Importin-beta-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100825040</name>
        <name evidence="2" type="ORF">BRADI_4g16893v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003577449.4"/>
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        <property type="protein sequence ID" value="KQJ88302"/>
        <property type="gene ID" value="BRADI_4g16893v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825040"/>
    <dbReference type="Gramene" id="KQJ88302">
        <property type="protein sequence ID" value="KQJ88302"/>
        <property type="gene ID" value="BRADI_4g16893v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825040"/>
    <dbReference type="OrthoDB" id="435593at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EL23">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057941">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF44">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24138">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="1">
        <location>
            <begin position="106"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ88302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1004" mass="108989" checksum="A5DFCB7E129AC7EC" modified="2016-01-20" version="1">MDGAEARDLQAQLAAAVQALNHGAHPSARLAANQWLLALQGSPQAWALAASLLASPDPSLPADLLFFAAQMLRRKIQSPSAPLPDPAAQLLDALLLAARRFCLGPPRLLTQICLALAALALRAEGGVDGLFARMQHLPDPALLELLTVLPEEVAQDQSGDTGVNAAARCRFTRELLAHAPAVLKFLLAQSEKPDGADGVSLHERSRRVLRCLLSWVRVGCFSGMPGAELAAHPLLTFAFNSLQVSSSFDVAIEVMTELVSQYQDLPQAFLSKILHIREVLLLPALANRSEKVIAGLTSLMCEVGLAAPALVAEGSNQAIALSDALLRCIAFSSEDWEIADSTLQFWCSLAHFLLGIDVQAAKRNATRELFLPVFSSLLDALLFRAQITDTDGVSTIPDGLAQFRLNLEELLVDICLLLGAPAYINKLLSGGGWGLSTQSIPWKEVEVRMYALSMVSDTILQDGSPLDFSIIMHFVNILSSRTPAELNGCHFLVYKSFGDVIGSYSKCLSSAKSNIKPLLLFCASGIAKSVSANACSLALRKLCEDASSFIHDPQNLEILFWISEGMDEGNLRIEDEEEIISAITHALCSVLDKELRKSSLSRLLCSSYSAVKKINDVDRDQSLRQGPGAYTQALNLAVRGLHRMGALFSHLAASVASGLIDDDTISVLLGIFWPLLEKLSKSSHMENTSLSAAACRSLSSAIHSCGQHFQILLPNILECLSTNFLLYQRHDCFLKTAANVIEEFGHKEEYSVVCVRTFETFSSAASLSNLNSSYTCDQEPDLVEAYVNFTSAFIRCCPKEVIFASGSLLELSFQKAAICSTAMHRGAALAAMSYLSCFLDVSLTAVLESPECLSDGSRDVVLVQILARCGEGLMSNVLYALLGVSALSRVHKSATVLQQLGALCSLCERTTWKAILCWDSLCRWLQSTVKSLPSEYLRHGEAEMIIQLWLKVLQDAASDYLHSRTVSNGRNHPGYMQGKGGRTLKRIIRDFAESHRNIPIPCPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ELV6</accession>
    <name>A0A0Q3ELV6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_4g19168v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ELV6"/>
    <dbReference type="EnsemblPlants" id="KQJ88535">
        <property type="protein sequence ID" value="KQJ88535"/>
        <property type="gene ID" value="BRADI_4g19168v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88535">
        <property type="protein sequence ID" value="KQJ88535"/>
        <property type="gene ID" value="BRADI_4g19168v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3ELV6"/>
    <dbReference type="OrthoDB" id="1667110at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3ELV6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF64">
        <property type="entry name" value="OS02G0828900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="26"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="117"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="699"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="736"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="708"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ88535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="797" mass="90233" checksum="B4B2DA85E3B60959" modified="2016-01-20" version="1">MGEEAEGTRASRLKWCHPCGFSDLGRLLPVGSPPPAIVGDGEEAKRGRARPRGRGRGRGRSGDRAFASPAFKPSDEARGVAAGNRVLRERRPAPQSFRERLDNQVLERLKKPVKSVAVKKLEPRKRKADQEGSKVQFSKGKIHSGNKGTSNNGVLEPAKKQERRDAEGAPNTKKLQPKEVDKLVPSSKNKSSGERYMNRKKMLTGENARMCDQCQRNDKERVVWCKACNNKRFCVPCITQWSVPPKKEISEENQIRYACHIVRLLLPWLGELQREQMEEKKLEASIRGVSVNEVKLEQVECDLDERVYCSMCKTSIFDFHRSCKNCLYDLCVICCRELRNGEIPGGQEVESMPYEDKGKDYVFAKKIQPNAENRRISLRRQMDSPNSPLPPWKANNDGSIPCPPKEIGGCSGSVLDVKCMFPEKVLAELESRTENAVRSELFAKETARRTANRKDSRDNHLYCPVATGIQDDDLVHFQMHWEKGEPVIVSDPLVMWRTLRERAQGREEDEQFAVKAIDCLDWCEIEINIHMFFMGYKTGRAHPRTFWPEMLKLKDWPPSSAFDKRLPRHGAEFISALPFPEYTDPRYGPLNLSVKLPSGVLKPDLGPKNYIAYGFSEELGRGDSVIATFLMRLPQIEKIKKNMRDQDLQELYGDLKSHSEIILKTSIVEPQNKSDDEAPKLLCGVENDDTHKDRCNGLQIDALPPGDNRSEAKDKAPPCKSLIQSGIHQGSDHIHEVNKSGETHNGAHCNSNNQGHPDWRVHQNKVSDPPTPVLKNSEQEKTGGALWDIFHREDSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ENH2</accession>
    <name>A0A0Q3ENH2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g23254v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ENH2"/>
    <dbReference type="EnsemblPlants" id="KQJ89054">
        <property type="protein sequence ID" value="KQJ89054"/>
        <property type="gene ID" value="BRADI_4g23254v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89054">
        <property type="protein sequence ID" value="KQJ89054"/>
        <property type="gene ID" value="BRADI_4g23254v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3ENH2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999500" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ89054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8825" checksum="14C9A91422E31D0D" modified="2016-01-20" version="1">MLSSLPSFGICMLQVAATWAGVTFATTTMHASSVDPPQIPPPETFLLGVPSRLTLLPLKDWCAVVDLHVRTNFHSPIMSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F0S7</accession>
    <name>A0A0Q3F0S7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g42273v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F0S7"/>
    <dbReference type="STRING" id="15368.A0A0Q3F0S7"/>
    <dbReference type="EnsemblPlants" id="KQJ92212">
        <property type="protein sequence ID" value="KQJ92212"/>
        <property type="gene ID" value="BRADI_4g42273v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92212">
        <property type="protein sequence ID" value="KQJ92212"/>
        <property type="gene ID" value="BRADI_4g42273v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3F0S7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
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        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ92212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="119" mass="12874" checksum="65861FA561BA47DD" modified="2016-01-20" version="1">MGSCGACWAFAATAALESHNTIRRKSLMPPSEQELVDYDSKSSGCQGGEASDASDYAFERVMLYDKFQPLQAVTHGPVVVPIAIGNNATSLEDYAYAAGLYLRPRRSDIDYEMLLIGCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FE39</accession>
    <name>A0A0Q3FE39_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g30761v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ97438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ97438">
        <property type="protein sequence ID" value="KQJ97438"/>
        <property type="gene ID" value="BRADI_3g30761v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97438">
        <property type="protein sequence ID" value="KQJ97438"/>
        <property type="gene ID" value="BRADI_3g30761v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FE39"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ97438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12297" checksum="3140F757FDA14A5F" modified="2016-01-20" version="1">MNHLLVDCPFSCVLWHEVLSWIRSICAPPTAGVCFADWWQESIHSSPPTMRKGTASLIMLTAWSIWKHRKAAVFDNATPNFASLLDLIKTDARCWASAGPAGLGMLLLAVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FSI1</accession>
    <name>A0A0Q3FSI1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E2F/DP family winged-helix DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100829412</name>
        <name evidence="12" type="ORF">BRADI_3g59740v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FSI1"/>
    <dbReference type="FunCoup" id="A0A0Q3FSI1">
        <property type="interactions" value="421"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FSI1"/>
    <dbReference type="EnsemblPlants" id="KQK01999">
        <property type="protein sequence ID" value="KQK01999"/>
        <property type="gene ID" value="BRADI_3g59740v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69664">
        <property type="protein sequence ID" value="PNT69664"/>
        <property type="gene ID" value="BRADI_3g59740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01999">
        <property type="protein sequence ID" value="KQK01999"/>
        <property type="gene ID" value="BRADI_3g59740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69664">
        <property type="protein sequence ID" value="PNT69664"/>
        <property type="gene ID" value="BRADI_3g59740v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5318at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FSI1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000073">
        <property type="entry name" value="E2F transcription factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000295">
        <property type="entry name" value="E2F transcription factor-like E2FE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015633">
        <property type="entry name" value="E2F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081:SF7">
        <property type="entry name" value="TRANSCRIPTION FACTOR EFL-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="11">
        <location>
            <begin position="46"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="11">
        <location>
            <begin position="190"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="133"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="349"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK01999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="422" mass="46669" checksum="A4C2CFCA7EF8E4C8" modified="2016-01-20" version="1">MATTSSSPTAEASAETAAAPPRPWPEPEPPLPLVFAAKLRDHAYSRKHKSLGLLCSNFVAMYDRDGVECIGLDDAARRLGVERRRIYDIVNVLESVGILARKAKNRYCWIGFGGVPMALRELKERAIRERSGLAPLPVEEPSAANMSDDEDDDKLGNPEGDTENERPSQTLDNLSDKPCAPICRLRSDHRKEKSLGLLTQNFVKLFLTMEVDTITLDEASKLLLGEGHEESNMKAKVRRLYDIANVLSSLNFIEKTQADTRKPAFRWLGTAGKAKPENGVTVAVDPQRKTMSNKRAFGTELTNIGINRSKVDSTVQKKAKLAQSGGDILKNDKIDVQSQVGPVKTSDFAYGPFHPTSARKQEPNGWHGAGQRESTQDWESLADSFRPQYQNQALGDLFSHYVEAWKSWHSEFAQAAGSCSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G3L4</accession>
    <name>A0A0Q3G3L4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Intimal thickness related receptor IRP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100834793</name>
        <name evidence="11" type="ORF">BRADI_2g23027v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753528.1">
        <property type="nucleotide sequence ID" value="XM_014898042.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G3L4"/>
    <dbReference type="FunCoup" id="A0A0Q3G3L4">
        <property type="interactions" value="1440"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK05863">
        <property type="protein sequence ID" value="KQK05863"/>
        <property type="gene ID" value="BRADI_2g23027v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834793"/>
    <dbReference type="Gramene" id="KQK05863">
        <property type="protein sequence ID" value="KQK05863"/>
        <property type="gene ID" value="BRADI_2g23027v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834793"/>
    <dbReference type="OrthoDB" id="29657at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3G3L4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054103">
        <property type="entry name" value="CAND6-7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF54">
        <property type="entry name" value="OS05G0462500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21904">
        <property type="entry name" value="CAND6-7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129128" description="Intimal thickness related receptor IRP domain-containing protein" evidence="8">
        <location>
            <begin position="19"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="CAND6/7 N-terminal" evidence="10">
        <location>
            <begin position="33"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="9">
        <location>
            <begin position="187"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK05863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="51081" checksum="8C6835753760B985" modified="2016-01-20" version="1">MAAGASLLLLLLLAVAGAIVPPSAAEIRSESFREDPRQSILFEKFGFSKSGAVRIILTGAAVSSSFARADPKQIGFFLLADESLLQAIYESRERPAPEKRAEDPTGADEPDLSSCVLSSPYVKTLFTFHDMEGGHYNKSFPVTHPDEYSLFFANCAPASLVTMNVRTEMYNANPDGSKDYLPVGQAPLPAIYGFFAFCYAAFLAAWGYLTLVSRDRASASNQIHHLMTALLVARMLYCLSAAEDQHYIRVTGTPHGWDVAFYLFQLVKGVILFAVIVLVGTGWSFLKPFLQDREKKVLMVVIPLQVMANIAAAVIGETGPFMQGWVTWNQILMFIDVACCCAVLFPVVWSMRSLRETSKTDGKAARNLSKLALFRQFYTVVIGYLYFTRIIVYALRTIASYRYRWASILAEEVATMAFYMFMFYTFRPAERSNYFSLDDDEEEAAEMVLREEEFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G4Q6</accession>
    <name>A0A0Q3G4Q6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BRADI_2g25710v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G4Q6"/>
    <dbReference type="FunCoup" id="A0A0Q3G4Q6">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3G4Q6"/>
    <dbReference type="EnsemblPlants" id="KQK06320">
        <property type="protein sequence ID" value="KQK06320"/>
        <property type="gene ID" value="BRADI_2g25710v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06320">
        <property type="protein sequence ID" value="KQK06320"/>
        <property type="gene ID" value="BRADI_2g25710v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3G4Q6"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3G4Q6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF211">
        <property type="entry name" value="CYTOCHROME P450-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999562" description="Cytochrome P450" evidence="13">
        <location>
            <begin position="18"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK06320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="53935" checksum="64688578D088796D" modified="2016-01-20" version="1" fragment="single">MAAISIFLLLLLAGVLAALRLAHSMLWVPRRLERRLRRQGIRGPPRSLISGNAADYVALLAAAQSTPLASFHHGIVARATPQYGEWPARYGRPFVFWLGPRPRLVVSGPDVAKAALTDATGAFEKGSPGGGNPLARQLIGEGLVGLTGEKWAHHRRIIAPAFNMERIKGWIPEISAITSSVLEKWEAQGETRAEFEIDVNKEFHTLSADVISSVAFGSSYEEGKRVFQLQEEQMKLVIIAIRSIYIPGFRFVPTKTNRRRHSLNKEIRNSLRKLIEINGSKCEDSKNLLGLMLSASKMENELKMGIEEIIDECKTFYFAGKETTANLLTWATLLLALHTEWQDKARDEVLQVCGKNEHPNAENLSSLKIVNMVLKETLRLYPPATFLNRMVTRDVELGKLDIPAGTQLNLPIIDIHHDADIWGANVEEFDPSRFADGKSYQLGAYFPFGIGPTICVGQNLAMVEAKLILAMVLQRFKFDVSPNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G6H2</accession>
    <name>A0A0Q3G6H2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g31733v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G6H2"/>
    <dbReference type="EnsemblPlants" id="KQK06960">
        <property type="protein sequence ID" value="KQK06960"/>
        <property type="gene ID" value="BRADI_2g31733v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06960">
        <property type="protein sequence ID" value="KQK06960"/>
        <property type="gene ID" value="BRADI_2g31733v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3G6H2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="117" mass="12459" checksum="C8449E6646E01769" modified="2016-01-20" version="1">MVARGHALGVVASGTEAHDHRMEQTADGTELCGHGDDAAIGDTDASDQPSNTATCFSFMQATREIMESISVLDRHGHSFPPLNFPAQSCLLTGEQVLFVALASARINEYCSLLIRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
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    <name>A0A0Q3G8E0_BRADI</name>
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            <fullName evidence="5">F-box domain-containing protein</fullName>
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        <name evidence="2" type="ORF">BRADI_2g36680v3</name>
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    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G8E0"/>
    <dbReference type="EnsemblPlants" id="KQK07631">
        <property type="protein sequence ID" value="KQK07631"/>
        <property type="gene ID" value="BRADI_2g36680v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07631">
        <property type="protein sequence ID" value="KQK07631"/>
        <property type="gene ID" value="BRADI_2g36680v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="693760at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="FBL15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709:SF52">
        <property type="entry name" value="OS07G0548100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709">
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK07631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="50536" checksum="2759D180B6CBCB03" modified="2016-01-20" version="1">MEHRGGEIAAKQPCLSTGGDGGSEDRLSALPDDVLIHILTISKLDAHFAGQTSVLSSRWRRLWPFLPELRFPVSSDPHRIRAALTAHEVSTLRFLVAGFRDATAESLVPWLPIATRRLSGDLILYNMAQRNQGERGGTLVLPCFEDATSFCLDLGHLGLSALNDGVFARLTDLCLARVHLHGPCMLGEVVSSPRCPSLRKLFVSDAWGLGNRAIHSDSLLEMEMKNLWQDSATGFGNLTIHSESLLKIKLVKLHGLQQLTVMAPSLKQLTVAACFASNGVILGPSRPVANISAPQLVSLDWRDAYDPSSVHVGEMTHLQQLGTNFFFGCNRLLRHFQHIRSLYLMLAYMPDIGDHQYLMEDITRLPDITFMGLGILSHGHSFGASVFHVLRLCIGVRKLSLTLVCPLLQEQTGCPSGCICGQPPNWKNEELSLNSLQEVELDSCQGTEHEAAPVKRLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GZ49</accession>
    <name>A0A0Q3GZ49_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g27021v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16144.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GZ49"/>
    <dbReference type="STRING" id="15368.A0A0Q3GZ49"/>
    <dbReference type="EnsemblPlants" id="KQK16144">
        <property type="protein sequence ID" value="KQK16144"/>
        <property type="gene ID" value="BRADI_1g27021v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16144">
        <property type="protein sequence ID" value="KQK16144"/>
        <property type="gene ID" value="BRADI_1g27021v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GZ49"/>
    <dbReference type="OrthoDB" id="667615at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055312">
        <property type="entry name" value="FBL15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709:SF76">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709">
        <property type="entry name" value="OS10G0396666 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="59"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK16144.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="354" mass="39260" checksum="CBA3D57D5E6C5CC8" modified="2018-03-28" version="2">MDSDHGATSIDVRRRINSLLDQILSLNGIRTFTFDDDKTTTIQRHHYDDGGVEDEEESEDRISILPDDLLHHILSFVPTQDAVRTSALSRRWRHVWIGLPALAFDDERSTTGFAESVDRVLVHGRHSHYIGDRIEISLAHPPHLAAAAAARSISIIFFREKKDCDRRHGVVLELPACANGRTTSMTLDFGVVGVGTLAIPPAVASAPSSLTQLELKNLRVDGSAFSHFVSSCCPHLRKLALYAFGYEDVLRVSNDALEALELGYEYGRGGLRRLEVSCRNLRRLRIANLFSPGVMARRVHDGSKAACFRTPRLEDLTWCSSAMILTSQQGGVRPQLGHRTTASRCSLHPRYGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H1F5</accession>
    <name>A0A0Q3H1F5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g03472v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81851.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H1F5"/>
    <dbReference type="EnsemblPlants" id="KQJ81851">
        <property type="protein sequence ID" value="KQJ81851"/>
        <property type="gene ID" value="BRADI_5g03472v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81851">
        <property type="protein sequence ID" value="KQJ81851"/>
        <property type="gene ID" value="BRADI_5g03472v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H1F5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ81851.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="126" mass="13588" checksum="E0B89A401369C292" modified="2018-03-28" version="2">MMSITLVTMCHSSQVILISSANEPPCSAAIRVLKLILWFGSLGATPSLSLLLMVAPIGALAAIWTQHLDSLDLGLIDLQDSSPVSAELMHNIINALFLTCSTYSCSLNSWCVSEILWLCCTFCLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H4Q7</accession>
    <name>A0A0Q3H4Q7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Subtilisin inhibitor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g17850v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H4Q7"/>
    <dbReference type="EnsemblPlants" id="KQJ88426">
        <property type="protein sequence ID" value="KQJ88426"/>
        <property type="gene ID" value="BRADI_4g17850v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88426">
        <property type="protein sequence ID" value="KQJ88426"/>
        <property type="gene ID" value="BRADI_4g17850v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H4Q7"/>
    <dbReference type="OrthoDB" id="618207at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.10">
        <property type="entry name" value="Trypsin Inhibitor V, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000864">
        <property type="entry name" value="Prot_inh_pot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036354">
        <property type="entry name" value="Prot_inh_pot1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091">
        <property type="entry name" value="PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091:SF90">
        <property type="entry name" value="SUBTILISIN INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00280">
        <property type="entry name" value="potato_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00292">
        <property type="entry name" value="POTATOINHBTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54654">
        <property type="entry name" value="CI-2 family of serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00285">
        <property type="entry name" value="POTATO_INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ88426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="6903" checksum="348C91D0A1310084" modified="2016-01-20" version="1">MSTTKTSWPEVVGWPAAQAVTQVGTDRPDVAIEVLPTGTSVAPGFNSKRVRVFFDGTGSVAATPVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H5T0</accession>
    <name>A0A0Q3H5T0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g40206v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H5T0"/>
    <dbReference type="EnsemblPlants" id="KQK18264">
        <property type="protein sequence ID" value="KQK18264"/>
        <property type="gene ID" value="BRADI_1g40206v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18264">
        <property type="protein sequence ID" value="KQK18264"/>
        <property type="gene ID" value="BRADI_1g40206v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H5T0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK18264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="212" mass="22678" checksum="6A1179DD45B9B3C4" modified="2016-01-20" version="1">METEGNGLSSSRHCSSVPLATNYRERYLQIKKGWDNLKVGVGGGGNIVAEAERRSELEAWSWPTRKTSEKNSTDYQINPSSCALIQSPNPGLLPAALQIGAAAPSSPRCSRLLPSPFRSAPVSSLPSSARLLLCVRRHPSSLPPPPPALRRRPETRPGNGQRRFEQPAATAATLSSGGDALLGTDGGGILLFYLRYGLSTEDAAFKVQSRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H735</accession>
    <name>A0A0Q3H735_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g42033v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H735"/>
    <dbReference type="EnsemblPlants" id="KQK18381">
        <property type="protein sequence ID" value="KQK18381"/>
        <property type="gene ID" value="BRADI_1g42033v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18381">
        <property type="protein sequence ID" value="KQK18381"/>
        <property type="gene ID" value="BRADI_1g42033v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H735"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999608" description="Secreted protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK18381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11989" checksum="63E7D9787689F384" modified="2016-01-20" version="1">MKYNSLTPVFLSLLHSHPLLTTCDEIPPPCSDTTCLGLAERCVHEQWITFLSDPVPCFLIRVLHGMPTEAYSVSARGYQEPVRLPGACTRAPRRLNPTFAPLPLETAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HQA5</accession>
    <name>A0A0Q3HQA5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g30667v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HQA5"/>
    <dbReference type="EnsemblPlants" id="KQJ90299">
        <property type="protein sequence ID" value="KQJ90299"/>
        <property type="gene ID" value="BRADI_4g30667v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ90299">
        <property type="protein sequence ID" value="KQJ90299"/>
        <property type="gene ID" value="BRADI_4g30667v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HQA5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ90299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="74" mass="9947" checksum="90DE3A89DCA7EA1F" modified="2016-01-20" version="1" fragment="single">WWCWRSWLWFRLSIRVRFSIWIGRRFISGFRCNFRWRLLCRRIRLWSRARPRIWIWSGFWLGPWTRLWLWFRLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HTF5</accession>
    <name>A0A0Q3HTF5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Pectinesterase</fullName>
            <ecNumber evidence="3 10">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100829331</name>
        <name evidence="12" type="ORF">BRADI_4g38840v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Acts in the modification of cell walls via demethylesterification of cell wall pectin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pectinesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="3 10"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91587.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576885.2">
        <property type="nucleotide sequence ID" value="XM_003576837.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HTF5"/>
    <dbReference type="STRING" id="15368.A0A0Q3HTF5"/>
    <dbReference type="EnsemblPlants" id="KQJ91587">
        <property type="protein sequence ID" value="KQJ91587"/>
        <property type="gene ID" value="BRADI_4g38840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829331"/>
    <dbReference type="Gramene" id="KQJ91587">
        <property type="protein sequence ID" value="KQJ91587"/>
        <property type="gene ID" value="BRADI_4g38840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829331"/>
    <dbReference type="OrthoDB" id="2019149at2759"/>
    <dbReference type="UniPathway" id="UPA00545">
        <property type="reaction ID" value="UER00823"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HTF5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000013">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF73">
        <property type="entry name" value="PECTINESTERASE 14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pectinesterase catalytic" evidence="11">
        <location>
            <begin position="86"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ91587.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="415" mass="43648" checksum="36F9C162E2AD2AC0" modified="2018-03-28" version="1">MHANAMPRTRTMRRASRSCSRAGRVIWASSAISSALLAILLASTHLAPSPSTNPPSLLDGRRRHCDEEAKWASSIASRLNITQILTVDRHGCGNFSSIQKAIDAVPDNNNHIGTRTLITVGAGTFHEKVTVWSNKTGLTIHGRGNLNSVVAWDDTAGTTGGCTPCSATFTVLAAGFVAYNVTFRNAAPPAGPGASGGQAVALRVAGDQAAFHWCGFYSAQDTLLDEQGRHFFRGCYVEGSIDFIFGNGRSLYLGCTISSVAAAASGSNAGGITGSVTAQGRRTEAEKTGFAFVRCSVVGTGSVWLGRAWGAYATVVFAETYLAGIVAPEGWNDWNDPARQGSVMFGEYESSGPGANKDGRVAYARQLDRRQAAPFMDVDYIDGGQWALPPLALAAPLLALPHGDDSDRASGGQVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I8V0</accession>
    <name>A0A0Q3I8V0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g00156v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I8V0"/>
    <dbReference type="EnsemblPlants" id="KQK02227">
        <property type="protein sequence ID" value="KQK02227"/>
        <property type="gene ID" value="BRADI_2g00156v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02227">
        <property type="protein sequence ID" value="KQK02227"/>
        <property type="gene ID" value="BRADI_2g00156v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3I8V0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK02227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="260" mass="26974" checksum="F231A9F843AE5364" modified="2016-01-20" version="1">MKRKAHLVNGMNSAEYHMGSSSSSNTRFSNSVQQNPTALSEPSHPQMPLSIGPSNWNGQSLVNQEAPRRNVRARHNPEPRPASTSTAINNNLPTFGSTGSAFTSTSAGRNQAPFSLPTRTLPSGASGVTGTVSSSRLYHPAMHSNSSIVAAATTVQGSSGGAIFANAGYAHPSSAASSSSRAISHAAVIPSYHPTTSSSVRINQPSPIGTAASSTHARHVSMGHANSGRNQRATNSIGLCWSSWLSTNQGNHHLLTPTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IAG8</accession>
    <name>A0A0Q3IAG8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BRADI_2g03853v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IAG8"/>
    <dbReference type="FunCoup" id="A0A0Q3IAG8">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3IAG8"/>
    <dbReference type="EnsemblPlants" id="KQK02811">
        <property type="protein sequence ID" value="KQK02811"/>
        <property type="gene ID" value="BRADI_2g03853v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02811">
        <property type="protein sequence ID" value="KQK02811"/>
        <property type="gene ID" value="BRADI_2g03853v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IAG8"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061:SF13">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="992"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="14">
        <location>
            <begin position="116"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="14">
        <location>
            <begin position="365"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK02811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1024" mass="112823" checksum="5EB0D7280958575C" modified="2016-01-20" version="1">MASFSRSREPPVLLLLLYLFIYLTLGVQLSYRSTVTPQYSNHTTAGNGVPAIPCLPDQASALLRLKRSFTVTNESRCTLASWQAGTDCCHWKGVHCRGFDGRVTSLHLGRCHLESAALDPSVFRLTSLRHLNLAWNDFNGSQLPASGFERLSELTHLNLSSSSFDGQIPVAIGHLTNLVSLDLSTTFFVQEESDEYISYATWSDHSLWLVEPNIASLLANLSNLRELNLDMVDLFDNGAAWCAAFSNSTTPQLQVLSLRYCLLQGHVCRSLSSIPTLTVINLQYNKLHGPIPEFLADLPSLSILQLTRNHLEGQFPVRIFENRNLTALDISYNFEVSGSLPNFSSDSCLANLVVSNTNFSGPIPSSIGNLKSLNKLGLAATGSSVQEIPSSIGELTSLDWLEISGAAVVGKLPSWVTKLTSLVHLQFSNCGLFGEIPSSISNLKKLEWLALYKCNFSGPIPPQIFNLTQLETIYLNSNNFAGTVELSSFLELPNLYHLSLSYNRLSVIDGEHNSILIPNWLSSLRLASCNISKFPDSLRNLQTLYYLDLSDNQIHGAIPHWVWDNRSVLNLSHNQFNSIGYDYALPIGISLFDLSSNLLEGPMPIPGPYTSSYDCSDNQFSSIPTNFGSQLSGVIYLKASGNNLSGEIPPSICDARDLALLDLSYNNLSGPIPSCLMEDLNSLRVLKLKANKLQGELPHRIKQGCGFYGLDLSDNQIEGQLPRSLVACRSLQVFDIGNNHINDTFPCWMSTLTELQVLVLKSNKFFGKVGTSVLGTAEENCEFMKLRILSLASNNFSSTLTNKWLKSLKSMTAKSTDDTSLMPNQHGLYLADGREHEFTAEITYKGYVVILNKILKTLVVIDVSDNGFNGVIPESVAELVLLCELNMSHNALTGTIPTQLGALHQLESLDLSSNDLSGEIPQELAWLDFLSVLNLSYNQLVGRIPGSCHFQTYSNLSFMGNIGLCGSPLSKECEDTTPNMMPHPWKREPMDIILFLFIGLGFGVGFAAAIVMWWGIRIRKPSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IHW6</accession>
    <name>A0A0Q3IHW6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g20123v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IHW6"/>
    <dbReference type="EnsemblPlants" id="KQK05451">
        <property type="protein sequence ID" value="KQK05451"/>
        <property type="gene ID" value="BRADI_2g20123v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05451">
        <property type="protein sequence ID" value="KQK05451"/>
        <property type="gene ID" value="BRADI_2g20123v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IHW6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK05451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="73" mass="8493" checksum="B8E2D6A7A28DAF83" modified="2016-01-20" version="1">MRWALSRSYRGRRLLPFRILRRRPAPPRIRRHLCPTVLDFLRGSAPEPARPVCLAPGHWKAKPALAPTTASWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IJS1</accession>
    <name>A0A0Q3IJS1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100830516</name>
        <name evidence="6" type="ORF">BRADI_3g51077v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family. Ago subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570049.2">
        <property type="nucleotide sequence ID" value="XM_003570001.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IJS1"/>
    <dbReference type="SMR" id="A0A0Q3IJS1"/>
    <dbReference type="FunCoup" id="A0A0Q3IJS1">
        <property type="interactions" value="2594"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3IJS1"/>
    <dbReference type="EnsemblPlants" id="KQK00676">
        <property type="protein sequence ID" value="KQK00676"/>
        <property type="gene ID" value="BRADI_3g51077v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830516"/>
    <dbReference type="Gramene" id="KQK00676">
        <property type="protein sequence ID" value="KQK00676"/>
        <property type="gene ID" value="BRADI_3g51077v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830516"/>
    <dbReference type="OrthoDB" id="10252740at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IJS1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009753">
        <property type="term" value="P:response to jasmonic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000110">
        <property type="entry name" value="Argonaute 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000013">
        <property type="entry name" value="protein argonaute 10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000001">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024357">
        <property type="entry name" value="Argonaut_Gly-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12764">
        <property type="entry name" value="Gly-rich_Ago1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="PAZ" evidence="4">
        <location>
            <begin position="415"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="5">
        <location>
            <begin position="704"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="23"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1030"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1030"/>
            <end position="1050"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK00676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1076" mass="119804" checksum="12836741E51E604B" modified="2016-01-20" version="1">MDFQMDNGHYSHQALTMVKNKQYAAISSGESSKTQEATRTPGQGPSQRVERGQQRGGSNRAHANTQYSQQGGRGGGHYQDPASQQPPGGPVKYQAHGYYGHGGPNQRGMSQPYHDGRRSGGGGRGVPITPSITLPELHQAPQVQHQVPVVTPSPRETGSSSLGVDMNTGQLQLQFQQLVNLGQSSSGQGIQLAPPSSKSVRFPMRPGKGKLGNRCIVKANHFSAELPDKDLHQYDVSITPDVPSRGVNRAVMGQLVTLFRQSHLGGSLPAYDGRKSLYTAGPLPFTSRTFEIILQDEEDRLGGAQAAQRREKHFTVVIKFAARADLHHLAMFLAGRQADAPQEALQVLDIVLRELPTARYSPVARSFYSPNLGRRQQLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEPLPVIDFVAQLLNRNVSVRPLSDADRVKIKKALRGVKVEVTHRGNMRRKYRISGLTSQATRELTFPIDNHGTVKTVVRYFQETYGFNIQHTTLPCLQVGNPQRPNYLPMEVCKIIEGQRYSKRLNEKQITALLKVTCQRPQQRELDILQTVNHNAYHEDPYAQEFGIRIDKKLASVEARILPPPRLKYHDSGREKDVLPRIGQWNMKNKKMVNGGRVKDWTCINFSRHVQDSAAKSFCHELAVMCQISGMEFSIDPLLPPLTARPEHVERALKARYQDSMTVLKPQGRELDLLIVILPDNNGSLYGDLKRICETDLGLVSQCCLTKHVFKMNQQYLANVALKINVKVGGRNTVLVNALSRRIPLVSDRPTIIFGADVTHPHPGEDSSPSIAAVVASQDWPEITKYAGLVSAQTRRQELIQDLFKVQQDPQRGSIAGGMVRELLISFKRSTGQKPQRIIFYRDGVSEGQFYQVLLYELDAIRKACASLEPNYQPPVTFVVVQKRHHTRLFANNHNDQHSVDRKSGNILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADGLQTLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYLEPDTSDSGSAMSGATTSRGPASARSNRAAGNVAVKPLPDLKDNVKRVMFYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J733</accession>
    <name>A0A0Q3J733_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100829911</name>
        <name evidence="2" type="ORF">BRADI_3g07440v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014755849.1">
        <property type="nucleotide sequence ID" value="XM_014900363.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J733"/>
    <dbReference type="STRING" id="15368.A0A0Q3J733"/>
    <dbReference type="EnsemblPlants" id="KQJ93912">
        <property type="protein sequence ID" value="KQJ93912"/>
        <property type="gene ID" value="BRADI_3g07440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829911"/>
    <dbReference type="Gramene" id="KQJ93912">
        <property type="protein sequence ID" value="KQJ93912"/>
        <property type="gene ID" value="BRADI_3g07440v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829911"/>
    <dbReference type="OrthoDB" id="695890at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3J733">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33564:SF32">
        <property type="entry name" value="DUF4005 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33564">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ93912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="144" mass="15202" checksum="6DDF39A1FDAF5F40" modified="2016-01-20" version="1">MSSSSSSSSIGASLALAAATAMALSGSLVLFSLRRFAKPAEHDDDVPASPASLRPCLSSSSSNSERRPRRKGEKKRVRFAAGVVDNEGAAPPVARSSSSARPSPSEPTCRGSAGGDHHRMPANREALYRGMLRDRSVHRTAYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J7M5</accession>
    <name>A0A0Q3J7M5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g08685v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J7M5"/>
    <dbReference type="EnsemblPlants" id="KQJ94128">
        <property type="protein sequence ID" value="KQJ94128"/>
        <property type="gene ID" value="BRADI_3g08685v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ94128">
        <property type="protein sequence ID" value="KQJ94128"/>
        <property type="gene ID" value="BRADI_3g08685v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3J7M5"/>
    <dbReference type="OrthoDB" id="638532at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999687" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ94128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="8019" checksum="ADDA0974993210B6" modified="2016-01-20" version="1">MLPPGTLLAVGFMLCPFRAFAPSGRNLTALLPELCELIGSLYRHVRGELVRQQLTGAVTSPRRHRRSLRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J8X3</accession>
    <name>A0A0Q3J8X3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="27 28">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LOC100824131</name>
        <name evidence="27" type="ORF">BRADI_1g15440v3</name>
    </gene>
    <organism evidence="27">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">Bd21</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Bd21</strain>
        </source>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="28">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Probably involved in the RNA silencing pathway. May cleave double-stranded RNA to produce short 21-24 nucleotides (nt) RNAs which target the selective destruction of complementary RNAs.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May interact with ARGONAUTE1 or PINHEAD through their common PAZ domains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18 20">Belongs to the helicase family. Dicer subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752438.1">
        <property type="nucleotide sequence ID" value="XM_014896952.2"/>
    </dbReference>
    <dbReference type="SMR" id="A0A0Q3J8X3"/>
    <dbReference type="FunCoup" id="A0A0Q3J8X3">
        <property type="interactions" value="1838"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3J8X3"/>
    <dbReference type="EnsemblPlants" id="KQK14323">
        <property type="protein sequence ID" value="KQK14323"/>
        <property type="gene ID" value="BRADI_1g15440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824131"/>
    <dbReference type="Gramene" id="KQK14323">
        <property type="protein sequence ID" value="KQK14323"/>
        <property type="gene ID" value="BRADI_1g15440v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3J8X3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010267">
        <property type="term" value="P:ta-siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18034">
        <property type="entry name" value="DEXHc_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd18802">
        <property type="entry name" value="SF2_C_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000004">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.380:FF:000001">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000420">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000705">
        <property type="entry name" value="Endoribonuclease dicer-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.380">
        <property type="entry name" value="Dicer dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038248">
        <property type="entry name" value="Dicer_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005034">
        <property type="entry name" value="Dicer_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF70">
        <property type="entry name" value="ENDORIBONUCLEASE DICER HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03368">
        <property type="entry name" value="Dicer_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51327">
        <property type="entry name" value="DICER_DSRBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0347">Helicase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="14 20" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="15" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="24">
        <location>
            <begin position="34"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="25">
        <location>
            <begin position="377"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Dicer dsRNA-binding fold" evidence="26">
        <location>
            <begin position="561"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="PAZ" evidence="23">
        <location>
            <begin position="811"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="22">
        <location>
            <begin position="953"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="22">
        <location>
            <begin position="1144"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="21">
        <location>
            <begin position="1317"/>
            <end position="1383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00056187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KQK14323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1402" mass="157798" checksum="253E5B290EDB2E87" modified="2016-01-20" version="1">MDDPLAAAAAAAASTEEAPKEDPQTIARWYQLEALERALCGNTVAFLGTGSGKTLIAVLLLRAYAHRVRSPARDFAVFLVPTVVLVDQQARVIEAHTDLRVDRFSGDMKVDYWDAPTWRRVVDDAEVLVMTPQILLNNLIHSFFRLQDIALLIFDECHRAKGNHPYACILKEFYHAQLNSRPQDPLPRIFGMTASPVNTKNLQPDTYLKRISQLENLLNSKVYTVDNESALSEYIQYATTRIVHYDDSIIPSNSYNHVMSCLNRLKEKHLEVLKANLYGSSLKVAKERISRLHRTFLYCTADLGIWLASKAAEVQSSNEQFLSFWGEQIEKDVEGFIRNYSEEVYRELSCCSKTGHIGEDLEANLQDGLLTRKVHCLVQTLLEYRHKQDLRCIVFVERVITSVVLKSLLSTITQMSGWIVKYMTGGISGLHSQSRKERTEILDSFRRGEVHLIVATQVLEEGLDVPSCNLVIRFDPPATVRSFIQSRGRARMQNSDYVLLVRRGDAAALSKTEKYLASGQLMREESLRLASTMCQPLENPLCEEDYYRVESTGATVTINTSISLIYLFCSKLPSDEYFKPLPRFSINKASCTCTLYLPKSSPVQTVSTEGEVDVLKKEVCLKACRELHAIGALTDSLLPELGFPCEEEPDIVVEKYQHEQPAYFPEELVDNWLSFSCLGIYICYMISLEGFLQTTAPTDIILAMKCDMGSDFVSRSFKLYGTQDYVTVAIKYMGIIHLNQEQVIIARRFQTSILSLLINNAIKYYYEMQRSIGIVYLLLPLVSGKIDWCTMKFSAPQEVNNKDVRHCHSCKEVDLLQTKDGLLCRCMLRNSIICTPHNSRLYAITGFLDLNAKSLLHLRDGSILTYITYFKTRHGLRLTNENQPLLAASKLGQVRNFLHKRNYKNEKVSSSSFAVELPPELCRVVMSPVPADILYSFSIIPSVMYRIHGMLLSAKLKVQLGPRMQQFSVPDLKILEALTTKECQEEFSQESLETLGDSFLKYVTTQHLYTKYKLHHEGTLTKMKKNLISNAALCQLACNNNLVGYIQGEVFNPKGWFIPGLGYDTCGNKSFCLSSNGMYSLRKISIKSKRIADTVEALIGAYLSAAGEQAAFLFINSLGMDIGFHSEIPIERNIVIKPEEFINIRSLEIILGYNFKDPLLLIEALTHGSYQIAGPTACYQRLEFLGDAVLDHLVTVYYYNTYPECTPALLTDLRSASVNNNCYAHASVKAGLNKHILHSSTELHRKMAYYLENFGQAFTGPSHGWEAGIGLPKVLGDVIESIAGAIYLDSKYDKEMVWRSMKPLLEPLATPETVERDPVKELQEFCDRRSYSRSYTKTHEGGVSSVVAEVQVEGTIYSATRSGRDKTVAQKLAVKSLLEDLKAAAAANDPIYLLPPLCNGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JDK1</accession>
    <name>A0A0Q3JDK1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g54222v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10396.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JDK1"/>
    <dbReference type="EnsemblPlants" id="KQK10396">
        <property type="protein sequence ID" value="KQK10396"/>
        <property type="gene ID" value="BRADI_2g54222v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10396">
        <property type="protein sequence ID" value="KQK10396"/>
        <property type="gene ID" value="BRADI_2g54222v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JDK1"/>
    <dbReference type="OrthoDB" id="690785at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK10396.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="60" mass="6076" checksum="88303173017B9E11" modified="2018-03-28" version="2">MAPSSYPAPAISKTPSGNPLPRRGQVKESMGKQIAAVAVSTASVVLHACSDKKAAGAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JPY1</accession>
    <name>A0A0Q3JPY1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g13868v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JPY1"/>
    <dbReference type="EnsemblPlants" id="KQK14004">
        <property type="protein sequence ID" value="KQK14004"/>
        <property type="gene ID" value="BRADI_1g13868v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14004">
        <property type="protein sequence ID" value="KQK14004"/>
        <property type="gene ID" value="BRADI_1g13868v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JPY1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="101" mass="11924" checksum="44CAF713F2F19A0E" modified="2016-01-20" version="1">MNKEFRRRKECHVVSDAWSSSMALFLVRLAESEKETGFCDTGCSRKMGFLLLYPRLHLLLVTYFLVFLRDLHDQIVELYTRSHFCLLIHDRSVLCQALKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JUP6</accession>
    <name>A0A0Q3JUP6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 4G</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Eukaryotic initiation factor 4F subunit p220</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100842805</name>
        <name evidence="11" type="ORF">BRADI_1g25002v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F. Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4G family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237155.1">
        <property type="nucleotide sequence ID" value="XM_010238853.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315361.1">
        <property type="nucleotide sequence ID" value="XM_024459593.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A0Q3JUP6">
        <property type="interactions" value="999"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3JUP6"/>
    <dbReference type="EnsemblPlants" id="KQK15811">
        <property type="protein sequence ID" value="KQK15811"/>
        <property type="gene ID" value="BRADI_1g25002v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK15812">
        <property type="protein sequence ID" value="KQK15812"/>
        <property type="gene ID" value="BRADI_1g25002v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842805"/>
    <dbReference type="Gramene" id="KQK15811">
        <property type="protein sequence ID" value="KQK15811"/>
        <property type="gene ID" value="BRADI_1g25002v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15812">
        <property type="protein sequence ID" value="KQK15812"/>
        <property type="gene ID" value="BRADI_1g25002v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842805"/>
    <dbReference type="OrthoDB" id="514777at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JUP6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
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                <person name="Shu S."/>
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                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Harmon-Smith M."/>
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                <person name="Grimwood J."/>
                <person name="Bruce D."/>
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                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="19"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575454.2">
        <property type="nucleotide sequence ID" value="XM_003575406.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3L1V1"/>
    <dbReference type="EnsemblPlants" id="KQJ86445">
        <property type="protein sequence ID" value="KQJ86445"/>
        <property type="gene ID" value="BRADI_4g05530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834425"/>
    <dbReference type="Gramene" id="KQJ86445">
        <property type="protein sequence ID" value="KQJ86445"/>
        <property type="gene ID" value="BRADI_4g05530v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834425"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000248">
        <property type="entry name" value="S-receptor-like kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF42">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="23">
        <location>
            <begin position="48"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="519"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="546"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQJ86445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="830" mass="92106" checksum="46B95E89CB812D6A" modified="2016-01-20" version="1">MQTVQLLRVFIIITLSFMFFYCFHQQLPCAHAISLPSSTAKNTSFLDYSTISLGGNSSARLFLQSRSSSNTLASFGLYSTDNSSFVLCVMMTYYSSGYADLVIGQPQVIWSANRNFPVGWNAILSFTGDGNLLLHNSDGTLVWSPETAGTSIVGGSLAVGMRLYGSGNLVLFDRNHSSVWQSFDYPTDTLVIGQSLCVGVNLISNASVTSWTSGQVNLHTRLNGLHFYFGSASYYKQVFQPTSLGNSTSQYCYAFANGSLGSPNLQIFSLPLARSFQFMRLELDGHLRLYEMEEATVRVVFDVLSNDVKFCDYPMACGEYGVCINGQCCCPSSSYFRLQDEWHPDVGCMPLTSLSCNHMGYHQLVPIGNISYFSDDSFQSLAASFSQKKKSLATSATDVYCKQSCLEECSCKVALFHYDSHDGNTGSCLLLSQALLLSQTKSSANHTLAFFKIQGSLPPKRRTSIAVGSAVGSLVLFSIAISIFIWRKCKKREEEEVYFGGIPGAPTRFSYNELKIATRNFSMKLGVGGFGTVFKGKIGKETIAVKRLEGVDQGKDEFLAEVETIGGIHHINLVRLVGFCAEKSHRLLVYEYMSNSSLDKWIFHAHPAFTLSWKTRRNIIMGIAKGLSYLHEECEQRIAHLDIKPHNILLDDKFQAKVSDFGLSKLISRDESKVMTRMRGTRGYLAPEWLGSKITEKVDIYSFGIVIVEIICGRENLDESQPEERIHLISLLQEKARSGQLLDLVDSSSNDMQFHLEEVREMMELAMWCLQVDSSKRPLMSTVAKVLEGAMALEATPNYDLVANYEPNHANVSGQICSYLPSATHLSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3L467</accession>
    <name>A0A0Q3L467_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100830270</name>
        <name evidence="11" type="ORF">BRADI_1g37667v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560649.1">
        <property type="nucleotide sequence ID" value="XM_003560601.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3L467"/>
    <dbReference type="STRING" id="15368.A0A0Q3L467"/>
    <dbReference type="EnsemblPlants" id="KQK17937">
        <property type="protein sequence ID" value="KQK17937"/>
        <property type="gene ID" value="BRADI_1g37667v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830270"/>
    <dbReference type="Gramene" id="KQK17937">
        <property type="protein sequence ID" value="KQK17937"/>
        <property type="gene ID" value="BRADI_1g37667v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830270"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3L467">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF216">
        <property type="entry name" value="ZINC TRANSPORTER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK17937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="408" mass="42650" checksum="6F4178B9B3880866" modified="2016-01-20" version="1">MAFFEGMESYAPYLHQFAASVSGTSCDRAAADEECRDDAAALRLKMVAVASILVAGAAGVAIPLVARKRRGGSGSGAGGGGTFVLAKAFAAGVILATGFVHMMHDAEEKFADPCLPSTPWRRFPFPGFVAMLAALGTLVVDFVGTSFYERKHRRDEEDASAAAAAARDESEATRFLLDDGVPSSRIAAAAVSGGDEKQDAMHIIGIRAHAAAHRHSHAHGHGACDGGAVFDGHGHGHEHGHADEEGPSQSRHVVVSQILELGIISHSVIIGLSLGVSQSPCTIKPLVAALSFHQFFEGFALGGCISEAQFKNFSALLMAFFFAITTPAGITVGAGIASFYNPNSPRALVVEGILDSMSAGILIYMALVDLIAADFLSRKMSCNPRLQVCSYVALFLGAMAMSSLAIWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LCI1</accession>
    <name>A0A0Q3LCI1_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g56755v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodium</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LCI1"/>
    <dbReference type="EnsemblPlants" id="KQK20785">
        <property type="protein sequence ID" value="KQK20785"/>
        <property type="gene ID" value="BRADI_1g56755v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20785">
        <property type="protein sequence ID" value="KQK20785"/>
        <property type="gene ID" value="BRADI_1g56755v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LCI1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK20785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="61" mass="6744" checksum="331A19A6FF4FFB92" modified="2016-01-20" version="1">MMDEQNGGQQLERKHNFYTTSEAATSSTSIGEIKSGGRTNHTKHRDLCVRIELNHTPGCGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LNE9</accession>
    <name>A0A0Q3LNE9_BRADI</name>
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        <recommendedName>
            <fullName evidence="5">YLP motif-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100838487</name>
        <name evidence="2" type="ORF">BRADI_3g08040v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571664.1">
        <property type="nucleotide sequence ID" value="XM_003571616.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LNE9"/>
    <dbReference type="FunCoup" id="A0A0Q3LNE9">
        <property type="interactions" value="671"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3LNE9"/>
    <dbReference type="EnsemblPlants" id="KQJ94021">
        <property type="protein sequence ID" value="KQJ94021"/>
        <property type="gene ID" value="BRADI_3g08040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838487"/>
    <dbReference type="Gramene" id="KQJ94021">
        <property type="protein sequence ID" value="KQJ94021"/>
        <property type="gene ID" value="BRADI_3g08040v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838487"/>
    <dbReference type="OrthoDB" id="513595at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LNE9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032204">
        <property type="term" value="P:regulation of telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000978">
        <property type="entry name" value="YLP motif-containing protein 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026314">
        <property type="entry name" value="YLP_motif_con_p1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13413">
        <property type="entry name" value="YLP MOTIF CONTAINING PROTEIN NUCLEAR PROTEIN ZAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13413:SF0">
        <property type="entry name" value="YLP MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ94021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="756" mass="84667" checksum="EC0BB5EC362F46DB" modified="2016-01-20" version="1">MDYPWRFPPGAGADLCPICSAPHFPFCPPPPLPPHPFPYDFHPPPPPPPPQYHAPFHPPPPQPPMWAPPGPHPYDLPDREGPHKRMRMGEAPPFDPYAPPPPPHPPMLGRDSVEGERLIGLIREHGHGHGRPPLPPTQRHGELYPHDGFGYEGARGYPPQNYINTYAQGGNSTDYDHERRLQGGNFADYDQARRLHPLPRHYDSGSDFVPVGGSREKYFDLDHHYHQFHQEASPGASSLPPVPHYAEAGNHYDSRDWRPHASVVPPPPDPPVPSPPDYHATPSLQAVNSSLFPVLSGSPATTALPPSDHTLHQSHLTLNANCCNGTDHSEGLDLIYRPHPEQHLRDRNPTQVKHSFNSTKLNTINACDLFKQPLRASRPDHIVIIMRGLPGSGKSYLAKALRDLEVECGGNAPRIHSMDDYFMIEVEKKVEGSEGSKSSTASKGRKQLTKKVIEYCYEPEMEETYRSSMLSAFMKTLDEGNFTFVIVDDRNLRVADFAQFWASAKKSGYEVYLLEAPYKDPTGCAARNVHGFTIDDIRKMAADWEEAPPLYLRLDTHSLFHDDNLREHSIQEVDMDMDDTDDANDTAVSTETENSNKAIPESTGVADQGEKWDSSDEEDLDDIKELGQSKWSKDFDDDTEKSKHTEGNTHALSGLAQTYGTRRKTLTWGDRLEKGGFSIGAAKRRRTSSLIIGPGSGYNLVSNPLDEDSSTETKDKVNNETKRRFCEQLRDEGQSFRAVFDKRKQRVGVFENGNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LVV3</accession>
    <name>A0A0Q3LVV3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g23045v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LVV3"/>
    <dbReference type="EnsemblPlants" id="KQJ96429">
        <property type="protein sequence ID" value="KQJ96429"/>
        <property type="gene ID" value="BRADI_3g23045v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96429">
        <property type="protein sequence ID" value="KQJ96429"/>
        <property type="gene ID" value="BRADI_3g23045v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LVV3"/>
    <dbReference type="OrthoDB" id="685486at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ96429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13609" checksum="1A8C3FC30D80E3DD" modified="2016-01-20" version="1">MTYMMKKLDLSETKRKGLMIHWKEGSTAREIEPKAVGELLLEKPTFASGLAQSWGKVQNMFLFTFHQTTGNRHAMEDGPWNFGNDLIVMENFVENKTIDEYEFLYVPIWIRVAMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3M621</accession>
    <name>A0A0Q3M621_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100834217</name>
        <name evidence="9" type="ORF">BRADI_3g46200v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318299.1">
        <property type="nucleotide sequence ID" value="XM_024462531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3M621"/>
    <dbReference type="STRING" id="15368.A0A0Q3M621"/>
    <dbReference type="EnsemblPlants" id="KQJ99949">
        <property type="protein sequence ID" value="KQJ99949"/>
        <property type="gene ID" value="BRADI_3g46200v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68849">
        <property type="protein sequence ID" value="PNT68849"/>
        <property type="gene ID" value="BRADI_3g46200v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834217"/>
    <dbReference type="Gramene" id="KQJ99949">
        <property type="protein sequence ID" value="KQJ99949"/>
        <property type="gene ID" value="BRADI_3g46200v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68849">
        <property type="protein sequence ID" value="PNT68849"/>
        <property type="gene ID" value="BRADI_3g46200v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2020857at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3M621">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080147">
        <property type="term" value="P:root hair cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000017">
        <property type="entry name" value="Transcription factor bHLH66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF215">
        <property type="entry name" value="OS02G0564700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="262"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="341"/>
            <end position="367"/>
        </location>
    </feature>
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        <location>
            <begin position="423"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ99949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="453" mass="48385" checksum="3FC93DACF4B64AF4" modified="2016-01-20" version="1">MGGFVDPFIPSPAWPQDMVFPGSSWAGSSAQGMGFHHLQNGSSPTQLITDKELAAATVFPAGNNAKLHDHEQQLLLRSTDDLDYTLCSILGGQNVSLTDSAPVLCSSSNESSGSEQSGAAGVLPQFLMGERHPVPVAWASGYAGEETPQSFGFPGDDLLHEAAPNGYQQLGTVAPAQLQLHDDAKFSAGKLLSFAPGQQQIRPDIDTLQQEFGSGLRHLNLVPGAQLAPFNPKQCAEDGRNGNGVAVGGGAPKARVRARRGQATDPHSIAERLRREKISDRMKNLQELVPNSNKTDKASMLEEIIEYIKFLQLQTKVLSMSRLGATDALVPLLMDSHNESSGLVMGSPKSGAAAGGKGHAGHRQTDSDDFVEDKVVLEQEVAQMMEDNMTMAMQYLQNRGLCLMPITLASAISVQKGTFSATVRPENGGKDDVKRVPTVLPSQKEVKSRPNVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3MYW3</accession>
    <name>A0A0Q3MYW3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g48061v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3MYW3"/>
    <dbReference type="EnsemblPlants" id="KQK09446">
        <property type="protein sequence ID" value="KQK09446"/>
        <property type="gene ID" value="BRADI_2g48061v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09446">
        <property type="protein sequence ID" value="KQK09446"/>
        <property type="gene ID" value="BRADI_2g48061v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3MYW3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK09446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="185" mass="20123" checksum="9E90C59772190620" modified="2016-01-20" version="1">MHEEEGVTRPSISCPSDVPRRFPPPPRLPPRRCTAPTAAPPSSCRCPPQDPVALQALSSRPPPSTSVSSAGPPSSRQCPPQDPAAPRALSSPRHRGLASAAHPRQRRCPPPPRAACLTSIWIGMRQGREIRERQEDLDWLLASIWIGIWCEGGRSRVCPMTGGPGRNCPAYGYPSLGMLQKKDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3N8A2</accession>
    <name>A0A0Q3N8A2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g06766v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12902.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3N8A2"/>
    <dbReference type="FunCoup" id="A0A0Q3N8A2">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK12902">
        <property type="protein sequence ID" value="KQK12902"/>
        <property type="gene ID" value="BRADI_1g06766v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12902">
        <property type="protein sequence ID" value="KQK12902"/>
        <property type="gene ID" value="BRADI_1g06766v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3N8A2"/>
    <dbReference type="OrthoDB" id="730395at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3N8A2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000444">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000158">
        <property type="entry name" value="pentatricopeptide repeat-containing protein At2g33680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000073">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF357">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="31"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="132"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="334"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="404"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="435"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="470"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK12902.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="672" mass="74468" checksum="8F1CB7C754AD98B6" modified="2018-03-28" version="2">MYVGSALIKMYADAGLLDGAREVFDGMDERDCVLWNVMMDGYVKAGDVASAVGLFRVMRASGCDPNFATLACFLSVCAAEADLLSGVQLHTLAVKYGLEPEVAVANTLVSMYAKCQCLEEAWRLFGLMPRDDLVTWNGMISGCVQNGLVDDALRLFCDMQKSGLQPDSVTLASLLPALTELNGFKQGKEIHGYIVRNCAHVDVFLVSALVDIYFKCRDVRMAQNVFDATKSIDVVIGSTMISGYVLNRMSEAAVKMFRYLLALGIKPNAVMVASTLPACASMAAMRIGQELHGYVLKNAYEGRCYVESALMDMYSKCGRLDLSHYMFSKMSAKDEVTWNSMISSFAQNGEPEEALDLFRQMIMEGVKYNNVTISSILSACAGLPAIYYGKEIHGIIIKGPIRADLFAESALIDMYGKCGNLELALRVFEHMPEKNEVSWNSIISAYGAHGLVKESVDLLCCMQEEGFKADHVTFLALISACAHAGQVQEGLRLFRCMTEEYHIEPQVEHLSCMVDLYSRAGKLDKAMQFIADMPFKPDAGIWGALLHACRVHRNVELAEIASQELFKLDPHNCGYYVLMSNINAVAGRWDGVSKMRRLMKDKKVQKIPGYSWVDVNNTSHLFVAADKNHPDSEEIYMSLKSLIIELKQEGYVPRPDVCYTTQPVDITQVMAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NKH9</accession>
    <name>A0A0Q3NKH9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100823529</name>
        <name evidence="12" type="ORF">BRADI_1g37377v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560635.2">
        <property type="nucleotide sequence ID" value="XM_003560587.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NKH9"/>
    <dbReference type="FunCoup" id="A0A0Q3NKH9">
        <property type="interactions" value="306"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK17886">
        <property type="protein sequence ID" value="KQK17886"/>
        <property type="gene ID" value="BRADI_1g37377v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823529"/>
    <dbReference type="Gramene" id="KQK17886">
        <property type="protein sequence ID" value="KQK17886"/>
        <property type="gene ID" value="BRADI_1g37377v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823529"/>
    <dbReference type="OrthoDB" id="10064100at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3NKH9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16655">
        <property type="entry name" value="RING-Ubox_WDSUB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01989">
        <property type="entry name" value="USP_STK_Ubox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051348">
        <property type="entry name" value="U-box_ubiquitin_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647">
        <property type="entry name" value="OS02G0152300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647:SF65">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="412"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="11">
        <location>
            <begin position="694"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK17886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="765" mass="84996" checksum="50B52B9068C5F237" modified="2016-01-20" version="1">MASSSSSTGGDPQAKPVAVAVAVRGDGRASRRAARWAAANLALVPGRVVLVHVIPPVSFVPSPSGERVPVERMEPGVVEMYAQDRRERAQEVFLPFRRFCGRRSVETVVLEGDSVSEALARYAAESGVRNLVLGSACLSWFRRILRLQNLPTTVLKATPCSCNVFIVSRRQLTVKFANLSQTGKSNTYVRIQSISHRAYALIQRNWLQVKHSLHGLPDDETPKSSGVTSSDLCSQACSSLSTSTNAGRKTPGRAGDKEFDAIGQLKEFPCVSLSSTEGPKPIDDVAKLRKELQNTLMTYGEAHEDVVHAKKKIQVLSNDCSEDLKEVQDALRREELLKQTAAYEKSKHFRAITDTEMVKEAFTCEAYSKHKTESVANMMSTETGKVVDALLCTGKTCRRYLRHEIELATDNFSDAKKIGEGGYGIVYRCTLDHTEVAVKVIQQDSSDKIDEFFKEVEILSQLHHPNLVLLLGFCPEIGCLVYEYMENGSLEDQLINNKGCQPLHWFMRFQIIFEVARGLAFLHGTKPEPIVHRDLKPGNILLDKNYVSKIGDVGFAKLIADLVPDGFTEYRDTVIAGTLYYMDPEYQLTGTVRPKSDLFALGIIVLQLLTGKHPHGLILSAEEAIRKDTFSDILDQSQTDWPIAEAETLAKLGLRCTALKCRDRPNLESEVLPVLEDLLSRVTSSLKSRSPNVVVPSHFVCPILQEVMDDPYVAADGHTYEYRAIKAWLKKHKISPVTKHKLPNSSIIPSHSLHAAIQRWKSQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R9Z1</accession>
    <name>A0A0Q3R9Z1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g51123v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09882.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R9Z1"/>
    <dbReference type="EnsemblPlants" id="KQK09882">
        <property type="protein sequence ID" value="KQK09882"/>
        <property type="gene ID" value="BRADI_2g51123v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09882">
        <property type="protein sequence ID" value="KQK09882"/>
        <property type="gene ID" value="BRADI_2g51123v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3R9Z1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK09882.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="103" mass="11532" checksum="4B4171F4C6330AC2" modified="2018-03-28" version="2">MLNLNPPGVLVTCVRTWPDAVDPRTENHARPTHWLTYRCARTCGCCSSSRSTCRHTINLLLDALLLLDYCGHKAQNASPKSPGWDWGEEAGSTNGRRSVHRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CFS1</accession>
    <name>A0A2K2CFS1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g07356v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CFS1"/>
    <dbReference type="EnsemblPlants" id="PNT60877">
        <property type="protein sequence ID" value="PNT60877"/>
        <property type="gene ID" value="BRADI_5g07356v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT60878">
        <property type="protein sequence ID" value="PNT60878"/>
        <property type="gene ID" value="BRADI_5g07356v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60877">
        <property type="protein sequence ID" value="PNT60877"/>
        <property type="gene ID" value="BRADI_5g07356v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60878">
        <property type="protein sequence ID" value="PNT60878"/>
        <property type="gene ID" value="BRADI_5g07356v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CFS1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT60877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="83" mass="9367" checksum="69D09EBA36A692E9" modified="2018-03-28" version="1">MKEGCASATQARVRWSFWTLGPDGIRGPAIEHFRKLSLPVQHGQVTRRAPHRREKLLIARSVMGRRILADMWIVDGQMCLGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CIS1</accession>
    <name>A0A2K2CIS1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100844425</name>
        <name evidence="22" type="ORF">BRADI_5g23020v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">Bd21</strain>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240477.2">
        <property type="nucleotide sequence ID" value="XM_010242175.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CIS1"/>
    <dbReference type="EnsemblPlants" id="PNT61919">
        <property type="protein sequence ID" value="PNT61919"/>
        <property type="gene ID" value="BRADI_5g23020v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844425"/>
    <dbReference type="Gramene" id="PNT61919">
        <property type="protein sequence ID" value="PNT61919"/>
        <property type="gene ID" value="BRADI_5g23020v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844425"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000014">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000402">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1045">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044576550" description="Receptor-like serine/threonine-protein kinase" evidence="18">
        <location>
            <begin position="29"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="20">
        <location>
            <begin position="29"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="21">
        <location>
            <begin position="343"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="505"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="532"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="PNT61919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="820" mass="90809" checksum="04C0CF03A497335B" modified="2018-03-28" version="1">MVGNGGGIMNMSYFPVFILLFLFSSCKSDDQLTQAKPLSPGNMLVSKEGTFALGFFSPANSNRNLYVGIWYNNIPERNRNILWVANRDKPATTTSSAMTTLMVSNSSNLVLLDLKGQTLWMTKNNMSAAQGLGGAYAVLLDTGNFVLRLPNGTIIWQSFDDPTDTALPGMRFLLSNKAHAVGRLVAWKGPNDPSPGEFSFSVDPSSNLEIITWNGTKPYCRIIVWNGVSVSGGTYLRNTSSVMYRTIINTGDMFYMMFTVSDGSPYTRVTLDYTGAFRILTWSNYSSSWTTISEKPSGSYGVYGSCGPFGYADFTGAVPTCQCLDGFKHDGLNSCQRVEELKCGKRSHFVALPGMRVPGKFLHIQNISFEQCAGECNRNCSCTAYAYANLSNAGTLADQTRCLVWTGELVDTWKTTFNGENLYIRLAGSPVHEKSSLAKTVLPIIACLLILCIAVVLRCKNRGKNKKILKKLMLGYLSPSSELGGENVEFPFLSFKDIISATHNFSDSCMLGRGGFGKVYKGILGDREVAIKRLSNGSGQGTEEFGNEVVLIAKLQHRNLVRLLGCCIHEDEKLLVYEYMPNRSLDAFLFDATRRYALDWLTRFKIIKGVARGLLYLHQDSRLTIIHRDLKASNILLDKEMSPKISDFGMARIFGGNQQQGNTIRVVGTYGYMSPEYVMSGAFSVKSDTYSFGVLLLEIVSGLKISSPQLITNFPNLTSYAWKLWEDGIARELVDSSVLDSCPLHEVLRCIHVGLLCVQDHSDARPLMSSVVFMLENETTFLPEPEQPAYFSPRNHENAHARETMENSFNAMSITTLVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CLC6</accession>
    <name>A0A2K2CLC6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100833105</name>
        <name evidence="5" type="ORF">BRADI_4g08750v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237332.1">
        <property type="nucleotide sequence ID" value="XM_010239030.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237333.1">
        <property type="nucleotide sequence ID" value="XM_010239031.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757926.1">
        <property type="nucleotide sequence ID" value="XM_014902440.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CLC6"/>
    <dbReference type="STRING" id="15368.A0A2K2CLC6"/>
    <dbReference type="EnsemblPlants" id="PNT62827">
        <property type="protein sequence ID" value="PNT62827"/>
        <property type="gene ID" value="BRADI_4g08750v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT62830">
        <property type="protein sequence ID" value="PNT62830"/>
        <property type="gene ID" value="BRADI_4g08750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833105"/>
    <dbReference type="Gramene" id="PNT62827">
        <property type="protein sequence ID" value="PNT62827"/>
        <property type="gene ID" value="BRADI_4g08750v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62830">
        <property type="protein sequence ID" value="PNT62830"/>
        <property type="gene ID" value="BRADI_4g08750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833105"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CLC6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044517">
        <property type="entry name" value="PHOX1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46183:SF3">
        <property type="entry name" value="OCTICOSAPEPTIDE_PHOX_BEM1P (PB1) DOMAIN-CONTAINING PROTEIN _ TETRATRICOPEPTIDE REPEAT (TPR)-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46183">
        <property type="entry name" value="PROTEIN CLMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PB1" evidence="4">
        <location>
            <begin position="529"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="332"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="483"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="334"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="367"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT62830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1014" mass="116431" checksum="4A83A80FF17EA3BF" modified="2018-03-28" version="1">MEEEMPGEKELSEPGSINCASSSSNSPCNSRISSQNNPNSLVDRDDDNISTGVVVELKEGMSTSELKEGATMELKEEGANIEVLKEEVTTTELKKGSAIDLMEEGVAINPVLEKEGVAIDLKKKEHMDTDLKEKGATFKESKEEGAPIELEKRSNHEKAIQLKRDGTRLFQQRDYEGAACAFDNAIKLLPKEHDDIAFLHCNIAACYMHMNPEDYERAIDECNSALEASPKYTKALLKRARCFEALDRLDLACKDAQKVLSLEPNNITALELFESIREEMEETEMLLENQLAVSLPEEPKSIVSAKERIKRKVSRRFRNNSIVEEEVWLIHDDEMQENDQDSKEEECDEENDHHMTSNLNNEENDSEEMRSKHDEDNCRYTEQQNHMKHSQYRQGPANDECQLLHHASWDMEEMHRKQTHSQNKALKELDVRSCQQHQQEMHPKQDQISGVNKRQKQIEEEIRRTSPSKNENRIEMYERHIKEKHSERHISRGEEDKQEKQMGIKVASHGKDQQYQQHIKEKKDSNKETRTVKFVLGDDIRIALVPENCSLIQLINIARCKYSPHLKAMLLKFKDIEGDLVTITSTEELRWVEDLKQGPARLYIKEVSPEREITRDIVMPSISIATLQKKHSISECGSSRHAAEEEKNSSYVDDWMVQFARLFKNHVGFDSDAYVDLRDLGTRLYYEAMEDTITSEEAQEIFHAAEAKFQEMAALALFNWGNIHMSRAKKRLVLSEDATKESVLSQVKSAYEWACAEYVKAGKKFEDTVDVKPDFYEGLIALGHQQFEQAKLSWRYADACKVDMGTEVLELFNRAEDNMEKGMEMWEGIEYLRVKGMSKSKKEKILLDKLGLDGHQQDLTADEAVEQASNMRSQLNISWGTILYERSVVELKLGLSSWEESLTEAIEKFKTGGASLADISVMVKNHCINEKTQEGLSFKIDEIVQAWNEMYDAKKLKNGWQLLGTVYIQEFQRVTFPIQTIRAAKNALVKLTIHFTSWTSTDVLKCIDCVLLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMC7</accession>
    <name>A0A2K2CMC7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_4g12735v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CMC7"/>
    <dbReference type="STRING" id="15368.A0A2K2CMC7"/>
    <dbReference type="EnsemblPlants" id="PNT63182">
        <property type="protein sequence ID" value="PNT63182"/>
        <property type="gene ID" value="BRADI_4g12735v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63182">
        <property type="protein sequence ID" value="PNT63182"/>
        <property type="gene ID" value="BRADI_4g12735v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CMC7"/>
    <dbReference type="OrthoDB" id="655162at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1227">
        <property type="entry name" value="OS11G0462500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="2"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="3">
        <location>
            <begin position="401"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="4">
        <location>
            <begin position="647"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="771"/>
            <end position="1128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT63182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1140" mass="128803" checksum="AE89E2053BD2EFC0" modified="2018-03-28" version="1">MKHLGSILGKCQNLQSLILDDASGSSSMSILCDGLSRVSPSPVHLQRIEVFPQACIFPSLPKWLGQLNSLCVLKIAVRELSKEDMDILKRLPALTAFSLYVGITPAEQIVFDKGFKVLWYFKFTCTALCMAFSEGTMSNVQRLKLRFNADKLEKYDPVRAGFEHLTGLKEISAKIGCIGADESDSKSALMDIFRKHPSRPIFNVQLVDRIIQGDKHKRVHMRVSLPREELSSYPQILGGKKRWRQMNVHLTKTLRGVTFFNCIYAEPLRGVTYLIMKLPLNLGDDLEFIKREMRQMSSIIEGNKEDDLEEEWVPQLQELVYDIEDYICIYSLLRTRLSVHIIPHMSHMKHLKGRIMSIQNWRHADISSKIIDTGSTAMPRSFPGTSPRCYVRQDDLIGIENPRDELLQLLIHADDRQPWMISIVGCRGSGKSTLAAVVYNDHRVCQEFDGRAWVVASECRNSGDLLDEIFQQLDPENKTTRSIYGLTCILQDRRCLVVIDQAERGEIWYDISRAFPPDSRIIVTTSVQSVVATAAGFFSSHSYIYAMQGLGDADSRKLFWRKVYGFDTEPAPALVDGSASIFKKCDGLPLALVSVANYVREHHQGGRIQRFGTNYQEAFGQMNRVFVQCFNSLPDNGHRTCLLSLSIFPHGHQINRKSVIRRWIAEGLVAGNAEHTAEEVAAARFSELINRSIIEPVLIGNNSKVKRCRVNGAMLEFVVNKAISKNFATLIRKDEPVSYTNPHPIRRLSVQGDGGTTGSGSIATGIGLSFIRSLTINNSEAFDFQRSKLLRVLDLERCKGLDDIVLDAVCQLLILKYLSLRGSDVRKIPKRIRRLRHLETLDIRGTKVNNLPMEAMMLPRLVHLFGRFELPEELRYEMEKSKLQKFLSEESRLQTLSGFVMEKNGGLESIVLHMSSLRKVKIWCKRYPCSTSAIEYDVSSIQKLFAGSNNALESISIDSDSYPTNFLHSLQGPCRISSIKLRGTLLQLPKFLSSVDSTLSELQLSFTGLSCEELSVLQNLRCLLYLKLSEVRQGLIGGIFEVRSGGFPSLERLCLEAPMLPEVHIKQGAMNNLTSLRLLCLPEYAGWRNVKGIEHLHRLKEVVLHHSVDAVGLEAWKQTAVKHENRPKVSREVVESIIPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQL6</accession>
    <name>A0A2K2CQL6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g27735v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQL6"/>
    <dbReference type="EnsemblPlants" id="PNT64338">
        <property type="protein sequence ID" value="PNT64338"/>
        <property type="gene ID" value="BRADI_4g27735v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64338">
        <property type="protein sequence ID" value="PNT64338"/>
        <property type="gene ID" value="BRADI_4g27735v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQL6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT64338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="49" mass="5242" checksum="72C4FC2246212D6E" modified="2018-03-28" version="1">MSCPLSEMIISVNKIKLSVGDRNNNPHSDSEICPLGLNKGRKGSISMVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CRS1</accession>
    <name>A0A2K2CRS1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_4g32267v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DPH4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CRS1"/>
    <dbReference type="FunCoup" id="A0A2K2CRS1">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CRS1"/>
    <dbReference type="EnsemblPlants" id="PNT64731">
        <property type="protein sequence ID" value="PNT64731"/>
        <property type="gene ID" value="BRADI_4g32267v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64731">
        <property type="protein sequence ID" value="PNT64731"/>
        <property type="gene ID" value="BRADI_4g32267v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CRS1"/>
    <dbReference type="OrthoDB" id="66964at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.660.10:FF:000003">
        <property type="entry name" value="DNAJ heat shock N-terminal domain-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT64731.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT64731"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
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        <name type="common">Purple false brome</name>
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    <reference evidence="2 3" key="1">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
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    <reference evidence="3" key="3">
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                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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            <begin position="84"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT66445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66445"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="126" mass="14258" checksum="7EF183E7CCB7AAA6" modified="2018-03-28" version="1">MVATKWVVPWPRASSQPPYKEHRCKAHACAPTAHQTAIDVDDRILRIKTLQTEPLRQNNHTVPIFPNTTLPCLLSNRRNPWCSIEEDDKGDQPAIRGTTPRREGQAACTRKKGLGRSLPLIDYNSH</sequence>
</entry>
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    <accession>A0A2K2CZI6</accession>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </feature>
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        </location>
    </feature>
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            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="128" mass="14229" checksum="63C01801765F1540" modified="2018-03-28" version="1">MRFDMMRRRRRTDGVRQQMDTSGAAAFAEERGRGGRRRAGSEDTGDSGRRRRCWRFIVPYLSRRPEALNCGLDERLQIASLRFGNVAGKDEHVGRAEIPAGFRPSSPPRPASRPSSRTGGGAGSRRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">BRADI_3g27537v3</name>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CZJ7"/>
    <dbReference type="EnsemblPlants" id="PNT67453">
        <property type="protein sequence ID" value="PNT67453"/>
        <property type="gene ID" value="BRADI_3g27537v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67453">
        <property type="protein sequence ID" value="PNT67453"/>
        <property type="gene ID" value="BRADI_3g27537v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CZJ7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="145" mass="14603" checksum="C5825EBF8564A98A" modified="2018-03-28" version="1">MDGAAPTPPPPPALTSLSAAAAPALTSTPLPPTSIGPPPTSTAAASAHRAGGRRHARPVQLPAAAPASSESFAAAAPPPSTTGPPPDLPDRRRLSSSTSFPLRRGLLVGFVVEEDRVVFLTGGPVCHCEILPDVVSCFISSGFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D5K8</accession>
    <name>A0A2K2D5K8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">histidine kinase</fullName>
            <ecNumber evidence="5">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BRADI_3g57807v3</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ethylene receptor family.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="5"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D5K8"/>
    <dbReference type="STRING" id="15368.A0A2K2D5K8"/>
    <dbReference type="EnsemblPlants" id="PNT69561">
        <property type="protein sequence ID" value="PNT69561"/>
        <property type="gene ID" value="BRADI_3g57807v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69561">
        <property type="protein sequence ID" value="PNT69561"/>
        <property type="gene ID" value="BRADI_3g57807v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D5K8"/>
    <dbReference type="OrthoDB" id="647750at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D5K8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051740">
        <property type="term" value="F:ethylene binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038199">
        <property type="term" value="F:ethylene receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000087">
        <property type="entry name" value="Ethylene receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058544">
        <property type="entry name" value="ETR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24423:SF628">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24423">
        <property type="entry name" value="TWO-COMPONENT SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25487">
        <property type="entry name" value="ETR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0186">Copper</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="18 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 20" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="22">
        <location>
            <begin position="565"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="20">
        <location>
            <position position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="PNT69561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="684" mass="74103" checksum="F8790BACA0484FF6" modified="2018-03-28" version="1">MTPQLLTAAQRYCGCAGDGAVEAMLQCQRVSDFLIAASYLSIPLELLYFASSRRADLVATAAPLDLRWLLLQLAAFAALGGATHLLAVFASHHPQSARILLASTAAKLLAALVSFATAATSRAVRMLAHVRGRDPQAILHAAMLHLADTLALRSCAVWMPGHDAGGILHMVHQLSLRGKGPVGVVLGSQAPISEDDPDVFDIKSSEAAKVLRPGSALATASSGGLMPPGAVAAIRMPMLKVSSFDGGKTPDQASSYAILVLVLHSQDGSREWGTQDLDIVQVVSDQVAVALSHATVLEEWQVMRDKLAEQRRALSRAKHEAVVANNAIKYVQSAMCDGMRRPMHSIIGVLSMVRQAENMRREQKPVVDAIARTSTLSLALMNDVDMETLVMNCAPFRLHSLIREAMSVAGCLASCRAVSFSYKLVNSLPEWVVGDETRVFQLLLHMVGDVLSRQCKNGGRLLFSVKGCNVDEEDCIPMQLNLSAGCSTCVKFQVGMERSTGCSQLPHRPVSSHISMCKKIVQIMNGTVRSASDGQSITLTLQFQLQQLGVCRRTPPSIPRFNGLRILLIDSDSMSRVVTQKLLEKLGYQVMSASSSIHCLALLESADSSFQLLLLDLDMDVFEVALQIRELRNRRWFLIAAALPVSIVDSIREMCRRSGINGLIQKPITLTALGAQLSRVLQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D626</accession>
    <name>A0A2K2D626_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g60446v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D626"/>
    <dbReference type="EnsemblPlants" id="PNT69727">
        <property type="protein sequence ID" value="PNT69727"/>
        <property type="gene ID" value="BRADI_3g60446v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69727">
        <property type="protein sequence ID" value="PNT69727"/>
        <property type="gene ID" value="BRADI_3g60446v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D626"/>
    <dbReference type="OrthoDB" id="638336at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1107">
        <property type="entry name" value="OS08G0373000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="3">
        <location>
            <begin position="138"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="258"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT69727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="637" mass="71819" checksum="372CE8C4349F59AF" modified="2018-03-28" version="1">MTSYSSRVITTTRMNNVAHSCCSSFSDHIYNIRPLGIVHSRQLFHRRLFNPEEDCFSYLEEVSEHILEKCAGLPLAIIAISGLLANIERTEGMWNQVKESMGHALERNCSVERMMRIFSLSYFDLPPRLKTCLLYLSIFPEDSIIESKDLIRRWIAEGFIHKEGRYTVSDLGERCFNELLNRSLIQPVKKDRYGNVKSCRVHDTVLDFINSKSTEENFVTLFGVPNLTVGTQGKVRRISLQVGKQGNCFIPTGLALSHVRSLSVFGDSVEIPFLDKFRHLRVLDFGGCYQLENHHLADIGRLFLLRYLNLRGTEVSELPEQISNLGCLEMLDLRSTMVHELPASIVSLKRLVHLFVDNDVTFPCGIAKMQALEMLKSVYVFKQTFNFLQELGQLKNLRKLLLDFQGDSAIGDTTGVHKEECRKSIVSSLGNLGRSENLGSLVIWEGGSFLQQGPMCPVPLSIHKLMTYRSRLPQVPKWVSSLLNLRSLCLQVEAITQEDFCILGALPALLILYLSGAAESKEGLRVSGEVGFRFLRMFRYYVKDGGMGLMFAVGGMPKLEKLAISTFFESSESESITISSDFGIRNLPCLITVRFNTNRRDKNIEATKAAMEEAASTHPNHPTVRFGIANSFSVKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D8K7</accession>
    <name>A0A2K2D8K7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100821594</name>
        <name evidence="2" type="ORF">BRADI_2g14492v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565813.1">
        <property type="nucleotide sequence ID" value="XM_003565765.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D8K7"/>
    <dbReference type="EnsemblPlants" id="PNT70616">
        <property type="protein sequence ID" value="PNT70616"/>
        <property type="gene ID" value="BRADI_2g14492v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821594"/>
    <dbReference type="Gramene" id="PNT70616">
        <property type="protein sequence ID" value="PNT70616"/>
        <property type="gene ID" value="BRADI_2g14492v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821594"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT70616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="131" mass="14618" checksum="4E5A074CEF422A52" modified="2018-03-28" version="1">MLPSIESSSSKELITTPSSSMSTQSPIVYAQDESSNNTPPVPQLAATDTDKDNLIKSAAKRSIQFTEHDLMNKGSENEVELTYGTKVQDVKRNKHETYQPCRKQHRKSQLHCLLPKISYSVAVIVLLESKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DP49</accession>
    <name>A0A2K2DP49_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g43343v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DP49"/>
    <dbReference type="EnsemblPlants" id="PNT76047">
        <property type="protein sequence ID" value="PNT76047"/>
        <property type="gene ID" value="BRADI_1g43343v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76047">
        <property type="protein sequence ID" value="PNT76047"/>
        <property type="gene ID" value="BRADI_1g43343v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DP49"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="97" mass="10814" checksum="12FDAE2AF451F32C" modified="2018-03-28" version="1">MSRRASCSVVISPRSTSTDKMIDTMPESDVRTATVAPRWPRPLPRGQDGRRRETRPRPGTSAWKMRRSGRTTCEGSVTSAIVTRTVTHGSRLWSFPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DPK7</accession>
    <name>A0A2K2DPK7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">K-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g45806v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DPK7"/>
    <dbReference type="STRING" id="15368.A0A2K2DPK7"/>
    <dbReference type="EnsemblPlants" id="PNT76211">
        <property type="protein sequence ID" value="PNT76211"/>
        <property type="gene ID" value="BRADI_1g45806v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76211">
        <property type="protein sequence ID" value="PNT76211"/>
        <property type="gene ID" value="BRADI_1g45806v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DPK7"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="K-box" evidence="2">
        <location>
            <begin position="23"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT76211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="159" mass="17405" checksum="CD9BC49682D34464" modified="2018-03-28" version="1">MAAVSLGSPMVANTVVRSSPLTMGVEHNKYNSLNEQLAEASLRLRHMRGEELEGLTVGELQQMEKNLETGLQRVLCTKDQQFMQQISELQQKGTLVAEENLRLRSQMPQVPKAGKMTVVETENVATEDVHSSESVMTALHSGSSHDNDDGSDISLKLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DPQ1</accession>
    <name>A0A2K2DPQ1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g46525v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DPQ1"/>
    <dbReference type="EnsemblPlants" id="PNT76263">
        <property type="protein sequence ID" value="PNT76263"/>
        <property type="gene ID" value="BRADI_1g46525v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76263">
        <property type="protein sequence ID" value="PNT76263"/>
        <property type="gene ID" value="BRADI_1g46525v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DPQ1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="17639" checksum="639BF09ACD7EEF04" modified="2018-03-28" version="1">MLKFEQEGGRQTHKTEATLHLEPADVVIVAVGTSPLGHEGTTARTWSGRSRHMFQHTVAPWSCTTRNTCVSPPPTQRGGGRRGERRGPRRCGARCRRKGRAGPWCRHSRGGRGPRTGILGTPGATSGGATSTRASGTCARTARTRGRRGAQIPGLHGGARTGGTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DS12</accession>
    <name>A0A2K2DS12_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_1g57297v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the plant thionin (TC 1.C.44) family. 4 C-C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DS12"/>
    <dbReference type="FunCoup" id="A0A2K2DS12">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77066">
        <property type="protein sequence ID" value="PNT77066"/>
        <property type="gene ID" value="BRADI_1g57297v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77066">
        <property type="protein sequence ID" value="PNT77066"/>
        <property type="gene ID" value="BRADI_1g57297v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DS12"/>
    <dbReference type="OrthoDB" id="653285at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DS12">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1350.10:FF:000001">
        <property type="entry name" value="Hellethionin-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1350.10">
        <property type="entry name" value="Thionin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001010">
        <property type="entry name" value="Thionin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036391">
        <property type="entry name" value="Thionin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33920">
        <property type="entry name" value="THIONIN-2.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33920:SF2">
        <property type="entry name" value="THIONIN-2.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00321">
        <property type="entry name" value="Thionin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00287">
        <property type="entry name" value="THIONIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57429">
        <property type="entry name" value="Crambin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00271">
        <property type="entry name" value="THIONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0800">Toxin</keyword>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT77066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="113" mass="12027" checksum="104A366BCF8FBC0E" modified="2018-03-28" version="1" fragment="single">RGRASCCKTTIARNCYNLCRAAGGARQVFASTCGCKIISGNKCPSNFRKMDLLPESGEPEAIKYCNIGCSSTVCDNMNHVFRGKEMKINVELCFDACVSLCNGTEAAVASVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DSG0</accession>
    <name>A0A2K2DSG0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)H dehydrogenase (quinone)</fullName>
            <ecNumber evidence="3">1.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100832004</name>
        <name evidence="7" type="ORF">BRADI_1g59440v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinone + NADPH + H(+) = a quinol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561515.2">
        <property type="nucleotide sequence ID" value="XM_003561467.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DSG0"/>
    <dbReference type="STRING" id="15368.A0A2K2DSG0"/>
    <dbReference type="EnsemblPlants" id="PNT77218">
        <property type="protein sequence ID" value="PNT77218"/>
        <property type="gene ID" value="BRADI_1g59440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832004"/>
    <dbReference type="Gramene" id="PNT77218">
        <property type="protein sequence ID" value="PNT77218"/>
        <property type="gene ID" value="BRADI_1g59440v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832004"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DSG0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF60">
        <property type="entry name" value="NAD(P)H DEHYDROGENASE (QUINONE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
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    </dbReference>
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        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="6">
        <location>
            <begin position="62"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT77218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="262" mass="27876" checksum="E013165B2C3E67A9" modified="2018-03-28" version="1">MAMHLISYIYLPTFSFFLHQNTHRRLVATSQEAEQFSNQIINKSNPTLQLAKDLSAMAATKIYIVYYSTWGHVATLAEEIKKGADTIPGVQATIWRVPETLPESVLSKMQAAPARENHGVITASQLADADGILFGFPTRFGMMAAQMKAFFDATGGLWHAGALAGKPAGFFFASGTQGGGQETTALTAVTQLAHHGMVFVPVGYTHGAGMFAMDEVKGGSPYGAGTFAGTDGSRVPSEAELALAEHQGKYFAGVAKKLKAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DTE2</accession>
    <name>A0A2K2DTE2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7731 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100828860</name>
        <name evidence="2" type="ORF">BRADI_1g64670v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561704.2">
        <property type="nucleotide sequence ID" value="XM_003561656.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DTE2"/>
    <dbReference type="STRING" id="15368.A0A2K2DTE2"/>
    <dbReference type="EnsemblPlants" id="PNT77546">
        <property type="protein sequence ID" value="PNT77546"/>
        <property type="gene ID" value="BRADI_1g64670v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77547">
        <property type="protein sequence ID" value="PNT77547"/>
        <property type="gene ID" value="BRADI_1g64670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828860"/>
    <dbReference type="Gramene" id="PNT77546">
        <property type="protein sequence ID" value="PNT77546"/>
        <property type="gene ID" value="BRADI_1g64670v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT77547"/>
        <property type="gene ID" value="BRADI_1g64670v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34366">
        <property type="entry name" value="OS07G0289901 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24865">
        <property type="entry name" value="DUF7731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7731" evidence="1">
        <location>
            <begin position="57"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT77546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="189" mass="20452" checksum="336423BDE50F4492" modified="2018-03-28" version="1">MGLIHTELLFSAVSPMGFLARLSRRWCGIAAVICLCASSCNPDVAPIEPLLLPDAPARSLQCFEDGQVYDCCEDAYRLNPSGIIGVPVSAVDYYCGGACVVETEDVLNCVASALDGFRFHNSASVEGVRYALRRGCSHTIKRGDFNDLEPPIGDYPDIYGDFGSDGYRFTVPLRMLTFLGGALLLVLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DTT2</accession>
    <name>A0A2K2DTT2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g66923v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DTT2"/>
    <dbReference type="FunCoup" id="A0A2K2DTT2">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77684">
        <property type="protein sequence ID" value="PNT77684"/>
        <property type="gene ID" value="BRADI_1g66923v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77684">
        <property type="protein sequence ID" value="PNT77684"/>
        <property type="gene ID" value="BRADI_1g66923v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DTT2"/>
    <dbReference type="OrthoDB" id="691358at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34670">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34670:SF8">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT77684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="9378" checksum="045FF4B994FCA00F" modified="2018-03-28" version="1">MEGLLPLVYRVIVQSRKGGEGPAVGNLFSDESLAAASTYRRLDTCDSGAYSPRPAAADASSLMLATVLSPRRLPIVNARESVHVHGIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DTU5</accession>
    <name>A0A2K2DTU5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100823099</name>
        <name evidence="8" type="ORF">BRADI_1g67290v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DTU5"/>
    <dbReference type="STRING" id="15368.A0A2K2DTU5"/>
    <dbReference type="EnsemblPlants" id="PNT77698">
        <property type="protein sequence ID" value="PNT77698"/>
        <property type="gene ID" value="BRADI_1g67290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77698">
        <property type="protein sequence ID" value="PNT77698"/>
        <property type="gene ID" value="BRADI_1g67290v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="167295at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DTU5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024491">
        <property type="entry name" value="Se_SelK/SelG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16875">
        <property type="entry name" value="SELENOPROTEIN K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16875:SF0">
        <property type="entry name" value="SELENOPROTEIN K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10961">
        <property type="entry name" value="SelK_SelG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="125"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT77698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="173" mass="18753" checksum="EBFE70CF5080B3AC" modified="2018-03-28" version="1">MDISQSDIIHPFVEIIAWLLNLFGFVSGADCFLWLYDCVFGLSLACKSVLFRVSLKWTDYYLEVNVFFIYLPGVVKGKRTIWRLSIIPDFFKAVLNFIRMFFLTMFSIEKTDSYKKGYGGGKKWDGGPGGGGPGGGPYGGGGGGGGGPRGPRTLSDLRSNDQSSLPACGSCCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DVN7</accession>
    <name>A0A2K2DVN7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100827038</name>
        <name evidence="5" type="ORF">BRADI_1g77791v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562203.1">
        <property type="nucleotide sequence ID" value="XM_003562155.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DVN7"/>
    <dbReference type="STRING" id="15368.A0A2K2DVN7"/>
    <dbReference type="EnsemblPlants" id="PNT78341">
        <property type="protein sequence ID" value="PNT78341"/>
        <property type="gene ID" value="BRADI_1g77791v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827038"/>
    <dbReference type="Gramene" id="PNT78341">
        <property type="protein sequence ID" value="PNT78341"/>
        <property type="gene ID" value="BRADI_1g77791v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827038"/>
    <dbReference type="OrthoDB" id="651791at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF396">
        <property type="entry name" value="BTB_POZ DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="191"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT78341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="394" mass="42182" checksum="E8E2421590993AD8" modified="2018-03-28" version="1">MGDNNESASTIVVTTTSASHVLDIKGYPKLIKLLPNGKGVASLPFAVQGRACTTSSWHVEFFPDGDLPARAGFVSLFAVLDRADSALKATVGFDLLDPATGDPVPRYASSAARDVVHDFTRPGAYSPHAGSSIRREELERVFRLLEAGGGSFSVRCNVTVFEFSGAAAAPAVPPPDLPRHLGGLLETREGADVSFLVGGAEDTTGCFSAHRCVLAARSPVFRAELYGGMAESAADGRCVRVDDMRPEVFGHLLRFAYTDSLPEPSDQEDADDHAIAMAQHLMEAADRYGMERLKLACEDTLCRHVGAGTGATTLALAEQHGCHRLKEACFEFLLKSPDALCSVMATDDFDHLSRSCPSLLKELICKLAARCSDPTEKPAEISPPCKRRKTKKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DVT0</accession>
    <name>A0A2K2DVT0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g78305v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DVT0"/>
    <dbReference type="EnsemblPlants" id="PNT78377">
        <property type="protein sequence ID" value="PNT78377"/>
        <property type="gene ID" value="BRADI_1g78305v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78377">
        <property type="protein sequence ID" value="PNT78377"/>
        <property type="gene ID" value="BRADI_1g78305v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DVT0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT78377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="81" mass="8977" checksum="AF79E460776D1FC1" modified="2018-03-28" version="1">MMSASYQLLFQKKLPTVHLLSATQNLNLHGPICSQKNVLRECTGNDSSKKRGKSTGNVQLKCQSDRRRSSKSGRCRVLSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-06-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C3SAB7</accession>
    <name>C3SAB7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Exostosin family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100836422</name>
        <name evidence="8" type="ORF">BRADI_1g78280v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2009" name="Plant Mol. Biol." volume="70" first="47" last="61">
            <title>Structural characterization of Brachypodium genome and its syntenic relationship with rice and wheat.</title>
            <authorList>
                <person name="Huo N."/>
                <person name="Vogel J.P."/>
                <person name="Lazo G.R."/>
                <person name="You F.M."/>
                <person name="Ma Y."/>
                <person name="McMahon S."/>
                <person name="Dvorak J."/>
                <person name="Anderson O.D."/>
                <person name="Luo M.C."/>
                <person name="Gu Y.Q."/>
            </authorList>
            <dbReference type="PubMed" id="19184460"/>
            <dbReference type="DOI" id="10.1007/s11103-009-9456-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="EU730901">
        <property type="protein sequence ID" value="ACF22751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562229.2">
        <property type="nucleotide sequence ID" value="XM_003562181.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C3SAB7"/>
    <dbReference type="STRING" id="15368.C3SAB7"/>
    <dbReference type="CAZy" id="GT47">
        <property type="family name" value="Glycosyltransferase Family 47"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK24126">
        <property type="protein sequence ID" value="KQK24126"/>
        <property type="gene ID" value="BRADI_1g78280v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836422"/>
    <dbReference type="Gramene" id="KQK24126">
        <property type="protein sequence ID" value="KQK24126"/>
        <property type="gene ID" value="BRADI_1g78280v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836422"/>
    <dbReference type="eggNOG" id="KOG1021">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012659_4_1_1"/>
    <dbReference type="OMA" id="ARAWPWQ"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF362">
        <property type="entry name" value="XYLOGLUCAN GALACTOSYLTRANSFERASE GT19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Exostosin GT47" evidence="6">
        <location>
            <begin position="39"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ACF22751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK24126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="462" mass="51678" checksum="4F7AF06429BBA9E0" modified="2009-06-16" version="1">MPRHLLITACNFLPILFFFFFFFSSPVVVLTAAALPDPCAGRRVHIRRLPPRFNTDLLGHCSTAFPLADHPSSTPSCASLANHGLGPRTHNRTRSWHRTDGRLLEPFFHRRVLDLPCLAADPAAADAVFLPYYASLDALPFVLEPAMLNFSAIHGVPLAQFLERDRPDVLKRNHGHDHFLVLAGPAWDYAQPPESEPRLWGTTSILRRPEFVNFTFLTLESRAWPWQEHAIPHPTAFHPPTFPRLQAWIARARRSRRTSLMLYAGGVSRPSKPNIRGSILAECANRTDNVCSLIDCSGGACALDPAHYMIPMLRSRFCLQPPGDTPTRRSTFDAVLAGCVPVFFEHASARTQYGWHLPPERYDEFSVTIPKDSVVLGGVVIAETLAAVPEVEVARMRARLLEMAPRVVYRRHGTSTAGEMGMDAIDIAVDGVLRRIRKRFKALQDGQAEAIYSMDDDNQEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1GM06</accession>
    <name>I1GM06_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosomal protein L30 ferredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100845883</name>
        <name evidence="7" type="ORF">BRADI_1g04950v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564261.1">
        <property type="nucleotide sequence ID" value="XM_003564213.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228147.1">
        <property type="nucleotide sequence ID" value="XM_010229845.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GM06"/>
    <dbReference type="STRING" id="15368.I1GM06"/>
    <dbReference type="EnsemblPlants" id="KQK12622">
        <property type="protein sequence ID" value="KQK12622"/>
        <property type="gene ID" value="BRADI_1g04950v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK12623">
        <property type="protein sequence ID" value="KQK12623"/>
        <property type="gene ID" value="BRADI_1g04950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845883"/>
    <dbReference type="Gramene" id="KQK12622">
        <property type="protein sequence ID" value="KQK12622"/>
        <property type="gene ID" value="BRADI_1g04950v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12623">
        <property type="protein sequence ID" value="KQK12623"/>
        <property type="gene ID" value="BRADI_1g04950v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845883"/>
    <dbReference type="eggNOG" id="KOG3184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055156_0_2_1"/>
    <dbReference type="OMA" id="SYYVDAQ"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000002">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="6">
        <location>
            <begin position="10"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="5">
        <location>
            <begin position="86"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="16"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK12623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="28353" checksum="C3163F26084DAAA1" modified="2012-06-13" version="1">MSSEAAKVVVPESVLRKRKREELWAAEKKTKAVEEKKKSSENRKVIFARAKQYAEEYDAQDKELVQLKREARLKGGFYVSPEAKLLFVVRIRGINAMHPKTRKILQLLRLRQIFNGVFLKVNKATINMLRRVEPYVAYGYPNLKSVRELIYKRGYGKLNKQRIPLTNNKVIEEGLGKHNIICIEDLVHEILTVGPHFKEANNFLWPFKLKAPLGGLKKKRNHYVEGGDAGNRENYINQLVRRMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMH1</accession>
    <name>I1GMH1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100825348</name>
        <name evidence="5" type="ORF">BRADI_1g06330v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559350.1">
        <property type="nucleotide sequence ID" value="XM_003559302.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMH1"/>
    <dbReference type="FunCoup" id="I1GMH1">
        <property type="interactions" value="1601"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK12841">
        <property type="protein sequence ID" value="KQK12841"/>
        <property type="gene ID" value="BRADI_1g06330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825348"/>
    <dbReference type="Gramene" id="KQK12841">
        <property type="protein sequence ID" value="KQK12841"/>
        <property type="gene ID" value="BRADI_1g06330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825348"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013137_15_4_1"/>
    <dbReference type="OMA" id="FCTRTPP"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000829">
        <property type="entry name" value="RING-H2 finger protein ATL70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045899">
        <property type="entry name" value="ATL71-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46719:SF7">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL71-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46719">
        <property type="entry name" value="TRANSCRIPTION FACTOR C2H2 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK12841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="204" mass="21537" checksum="B0418D3FFD0044E2" modified="2012-06-13" version="1">MSSSGGPPPPPGPGRQLNPPDGNSVGMFSSDRIGGFGYGVGVSVGILLLITTITLASYFCTRAPPAIADTDAAASTRRPRRRRPEDEDANANNSNGAEADLELGIDEATLKGYPEVVYGEAKGKKKKKPGASTTCTCCSVCLDNYGDGDVLRMLPDCGHLFHRECVDPWLRQHPTCPVCRTSPLPSPMPTPLAEVTPLAMARPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPS8</accession>
    <name>I1GPS8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100840672</name>
        <name evidence="8" type="ORF">BRADI_1g13060v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thaumatin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559612.2">
        <property type="nucleotide sequence ID" value="XM_003559564.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPS8"/>
    <dbReference type="FunCoup" id="I1GPS8">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GPS8"/>
    <dbReference type="EnsemblPlants" id="KQK13877">
        <property type="protein sequence ID" value="KQK13877"/>
        <property type="gene ID" value="BRADI_1g13060v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840672"/>
    <dbReference type="Gramene" id="KQK13877">
        <property type="protein sequence ID" value="KQK13877"/>
        <property type="gene ID" value="BRADI_1g13060v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840672"/>
    <dbReference type="eggNOG" id="ENOG502QV4N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043181_5_0_1"/>
    <dbReference type="OMA" id="CKGGTNY"/>
    <dbReference type="OrthoDB" id="430315at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000003">
        <property type="entry name" value="Thaumatin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0295">Fungicide</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094003" evidence="7">
        <location>
            <begin position="25"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="33"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="90"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="143"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="150"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="182"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK13877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="239" mass="25445" checksum="A45E9E8919A40F3D" modified="2012-06-13" version="1">MAMLNTSWVLRILPFFLLVAFTHAATFTITNKCQFTVWAAAVPSGGGKQLDAGQHWTIDVPAGTTGGRVWARTGCDFDGNGNGRCQTGDCGGVLQCTGYGQAPNTLAEFGLNKFNDLDFFDISLIDGFNVPMDFLPADGTAGCDKGGPRCDADVTAQCPSELKAPGGCNNACTVFKEDRYCCTGTAADNCGPTDYSRFFKGQCPDAYSYPKDDQTSTFTCPGGTNYQVVFCPQASERAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRQ9</accession>
    <name>I1GRQ9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL45</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L45, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100837234</name>
        <name evidence="11" type="ORF">BRADI_1g19550v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL45 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562558.1">
        <property type="nucleotide sequence ID" value="XM_003562510.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRQ9"/>
    <dbReference type="STRING" id="15368.I1GRQ9"/>
    <dbReference type="EnsemblPlants" id="KQK14923">
        <property type="protein sequence ID" value="KQK14923"/>
        <property type="gene ID" value="BRADI_1g19550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837234"/>
    <dbReference type="Gramene" id="KQK14923">
        <property type="protein sequence ID" value="KQK14923"/>
        <property type="gene ID" value="BRADI_1g19550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837234"/>
    <dbReference type="eggNOG" id="KOG4599">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073960_1_0_1"/>
    <dbReference type="OMA" id="RQSMWSS"/>
    <dbReference type="OrthoDB" id="19619at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GRQ9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.240:FF:000006">
        <property type="entry name" value="Mitochondrial inner membrane translocase complex, subunit Tim44-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051975">
        <property type="entry name" value="mtLSU_mL45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28554">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L45, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28554:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tim44-like" evidence="10">
        <location>
            <begin position="170"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK14923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="324" mass="36483" checksum="127D5055AB46326D" modified="2012-06-13" version="1">MSLARLGQSLIRRLHRPLHLPPPPPTDHHAAVSRSLALSQANRCLRGFASLTYNPGSTIAGKFGGPSPVHAVQLQVLERVVQLNHARPMSSAAPAPAGSTSSGPPETSPSVSKGVPVGARKVGMKVVMMSPGFVYEPYSLPEPISFWRRWFTRSGWTRTKEDIILEMKNAYAVSRLRKKTGYTKKQFYNQAFNIYKEVNTLMAQGDTSSLRKALTERMHSTMKNEIKRRQSKWKSVHWELVEPAVNIRTLRARMIGLDKNDLDKAFIQLTLEFITKQKFEAYNSKGEVVSGDKSKEVLVKDIWVFERSLFHPGADWRVCGRITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRR2</accession>
    <name>I1GRR2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100838065</name>
        <name evidence="2" type="ORF">BRADI_1g19570v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mo25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562561.1">
        <property type="nucleotide sequence ID" value="XM_003562513.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRR2"/>
    <dbReference type="STRING" id="15368.I1GRR2"/>
    <dbReference type="EnsemblPlants" id="KQK14925">
        <property type="protein sequence ID" value="KQK14925"/>
        <property type="gene ID" value="BRADI_1g19570v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838065"/>
    <dbReference type="Gramene" id="KQK14925">
        <property type="protein sequence ID" value="KQK14925"/>
        <property type="gene ID" value="BRADI_1g19570v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838065"/>
    <dbReference type="eggNOG" id="KOG1566">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035755_1_0_1"/>
    <dbReference type="OMA" id="AYDHKES"/>
    <dbReference type="OrthoDB" id="609103at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000247">
        <property type="entry name" value="calcium-binding protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013878">
        <property type="entry name" value="Mo25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182:SF3">
        <property type="entry name" value="PROTEIN MO25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08569">
        <property type="entry name" value="Mo25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="336" mass="38777" checksum="0D3FB4BD8D9F68B9" modified="2012-06-13" version="1">MSFFFRAASRPRSSQNELVRSIKDSLLALDTKTGAKALEDVEKNIFTLRQTLSGDGEVEPNQEQVLQIALEICKEGVLSLFVQNLPSLGWEGRKDLVHCWGVLLRQKVDESHCCVQYIENHVDLLDFLVVCYKNLEVALNCGNMLRECIKYPALAKYILKSSSFELFFQYVELPNFDIASDALNTFKDLLTRHEDAVSEFLSSHYEQFFELYTRILTSNNYVTRRQSVKFLSEFLLEAPNAQIMKRYILEVRYLNIMIGLLKDSSKNIRICAFHIFKVFVANPNKPRDIIQVLVDNHRELLKLLTDLPAGKGEDDQLDEERDLIIKEIQKLVRSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTI5</accession>
    <name>I1GTI5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MYB transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100841283</name>
        <name evidence="11" type="synonym">MYB41</name>
        <name evidence="10" type="ORF">BRADI_1g24887v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="journal article" date="2021" name="Plant J." volume="0" first="0" last="0">
            <title>BdFAR4, a root-specific fatty acyl-coenzyme A reductase, is involved in fatty alcohol synthesis of root suberin polyester in Brachypodium distachyon.</title>
            <authorList>
                <person name="Wang Y."/>
                <person name="Xu J."/>
                <person name="He Z."/>
                <person name="Hu N."/>
                <person name="Luo W."/>
                <person name="Liu X."/>
                <person name="Shi X."/>
                <person name="Liu T."/>
                <person name="Jiang Q."/>
                <person name="An P."/>
                <person name="Liu L."/>
                <person name="Sun Y."/>
                <person name="Jetter R."/>
                <person name="Li C."/>
                <person name="Wang Z."/>
            </authorList>
            <dbReference type="PubMed" id="33768632"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="MT681927">
        <property type="protein sequence ID" value="QTK16915.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560097.1">
        <property type="nucleotide sequence ID" value="XM_003560049.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GTI5"/>
    <dbReference type="FunCoup" id="I1GTI5">
        <property type="interactions" value="800"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK15782">
        <property type="protein sequence ID" value="KQK15782"/>
        <property type="gene ID" value="BRADI_1g24887v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841283"/>
    <dbReference type="Gramene" id="KQK15782">
        <property type="protein sequence ID" value="KQK15782"/>
        <property type="gene ID" value="BRADI_1g24887v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841283"/>
    <dbReference type="eggNOG" id="KOG0048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028567_15_2_1"/>
    <dbReference type="OMA" id="HANPMVQ"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000349">
        <property type="entry name" value="Transcription factor MYB39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994:SF5">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="9"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="66"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="293"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK15782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="QTK16915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="372" mass="41087" checksum="9EAF1E71CE5B98F6" modified="2012-06-13" version="1">MGRAPCCDRAGLKKGPWTQEEDEKLVSYIKKHGQGNWRTLPKNAGLERCGKSCRLRWTNYLRPDIKRGRFSFEEEETIIQLHSILGNKWSAIAARLPGRTDNEIKNYWNTHIRKRLLRMGIDPVTHAPRLDFLDLTSLLNKHHHPAATNAYYPTQADLEALRALEPLASYPADLLRLASTLLSPPNQDQQQQQQQMLLMPWLQIMAQQQQQQQQQVSDQRATAAAAHQLLMQQPCGLAAGQMPGLVHHANLQEDHMVPGCVVDYAQPPAAGACYDYVPALMHMVSDADASNNNNNNLQWSSTVDTGSNNNVGSGVSTPSSSPAAGRVNSASTTTTTTTAFGALNDVIAADDADAGLFDMQLYSDLLDVSDYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I1GUG6</accession>
    <name>I1GUG6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g27820v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GUG6"/>
    <dbReference type="STRING" id="15368.I1GUG6"/>
    <dbReference type="EnsemblPlants" id="KQK16257">
        <property type="protein sequence ID" value="KQK16257"/>
        <property type="gene ID" value="BRADI_1g27820v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16257">
        <property type="protein sequence ID" value="KQK16257"/>
        <property type="gene ID" value="BRADI_1g27820v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1429864_0_0_1"/>
    <dbReference type="InParanoid" id="I1GUG6"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="64"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK16257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="190" mass="21068" checksum="627E0FED4906B7DE" modified="2012-06-13" version="1">MGTNRTRFQFPNQRRRRYPVFIRDDGAGEGGGAAAQELHRHGGRVPRPRAQEQQQQEQDHAAGGPDVHSMGFFFTLNGIAMGQTTVVMTESAVRAGLRGVLDVAERSGITEIMAAAPVVVGMTREMRQHWRLLPALEHAICGGAQLPDSAAKQFRRRFLHLDLCMKNKSSNVSDPDPLHLIRESFESKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWB6</accession>
    <name>I1GWB6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-related modifier 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100822830</name>
        <name evidence="7" type="ORF">BRADI_1g33110v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 6">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of the MOCS3/UBA4 homolog, then thiocarboxylated (-COSH) via the rhodanese domain of the MOCS3/UBA4 homolog.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the URM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563439.1">
        <property type="nucleotide sequence ID" value="XM_003563391.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWB6"/>
    <dbReference type="STRING" id="15368.I1GWB6"/>
    <dbReference type="EnsemblPlants" id="KQK17225">
        <property type="protein sequence ID" value="KQK17225"/>
        <property type="gene ID" value="BRADI_1g33110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822830"/>
    <dbReference type="Gramene" id="KQK17225">
        <property type="protein sequence ID" value="KQK17225"/>
        <property type="gene ID" value="BRADI_1g33110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822830"/>
    <dbReference type="eggNOG" id="KOG4146">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148208_0_1_1"/>
    <dbReference type="OMA" id="DYELQPN"/>
    <dbReference type="OrthoDB" id="10248987at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01764">
        <property type="entry name" value="Ubl_Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03048">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015221">
        <property type="entry name" value="Urm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14986">
        <property type="entry name" value="RURM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09138">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037379">
        <property type="entry name" value="Ubiquitin-related_modifier_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="modified residue" description="1-thioglycine" evidence="5">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)" evidence="5">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK17225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="103" mass="11228" checksum="9F2E131E73F2940E" modified="2012-06-13" version="1">MHLTLEFGGGLELLLEKSTKVHKVDCQPTMDGSEGGKATMKGLLSWVKSNLIKERPEMFIKGDSVRPGVLVLINDCDWELCGGLDAELEDKDVVVFISTLHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1H222</accession>
    <name>I1H222_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100822667</name>
        <name evidence="11" type="ORF">BRADI_1g52230v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DExH box helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557276.1">
        <property type="nucleotide sequence ID" value="XM_003557228.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H222"/>
    <dbReference type="FunCoup" id="I1H222">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H222"/>
    <dbReference type="EnsemblPlants" id="KQK20065">
        <property type="protein sequence ID" value="KQK20065"/>
        <property type="gene ID" value="BRADI_1g52230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822667"/>
    <dbReference type="Gramene" id="KQK20065">
        <property type="protein sequence ID" value="KQK20065"/>
        <property type="gene ID" value="BRADI_1g52230v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822667"/>
    <dbReference type="eggNOG" id="KOG0948">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002902_0_1_1"/>
    <dbReference type="OMA" id="IMLKNYN"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H222">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000083">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000141">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.300:FF:000003">
        <property type="entry name" value="DEAD-box family ATP dependent helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000009">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025696">
        <property type="entry name" value="Beta-barrel_MTR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF25">
        <property type="entry name" value="DEXH-BOX ATP-DEPENDENT RNA HELICASE DEXH9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13234">
        <property type="entry name" value="MTR4_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="84"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="315"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK20065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="993" mass="112724" checksum="C9FCC05A10841C2A" modified="2012-06-13" version="1">METLKRKAPEVPAARANSPTKAPRDDDAPAAPAASEPVACVHDVSYPEGYDASTSSRIVAGGGEGVAPAKTFPFKLDPFQSEAIRCLDNGESVMVSAHTSAGKTVVALYVIAMSLRNQQRVIYTSPIKALSNQKYREFKEEFSDVGLMTGDVTIEPNASCLVMTTEIWRSMQYKGSEVMREVAWVIFDEVHYMRDRERGVVWEESIVMAPKNSRFVFLSATVPNAKEFADWVAKVHKQPCHIVYTDYRPTPLQHYVFPSGGDGLYLVVDENGKFREDSFQKSLNVLAPASGGDKKRENGKRQKGISAGKPGEESDIFKMVKMIIQRQYDPVILFSFSKRECEFLAMQMAKMDLNEDDEKVNIETIFWSAMDLLSDDDKKLPQVSNMLPLLKRGIGVHHSGLLPILKEVIEILFQEGLIKCLFATETFSIGLNMPAKTVVFTNVRKFDGDRFRWLSSGEYIQMSGRAGRRGIDDRGICILMVDDKMEPSTAKMMLKGGADSLNSAFHLSYNMLLNQMRCEDGDPEKLLRHSFYQFQADRALPDLEKRVRELEIERNSMVIDEEESLKDYYDLLEQYKTLKKDVRDIVLSPKYVLPFLQSGRLVRVQFSTDEQPTFSIDENVTWGIIINFEKVKTQAEDRKPEDCDYAVDILTRCSVNKDISGKKTMKIIPLKDRGEPVVISLPLSQIDGLSSVRMYIPKDLLPVEARENTLRKVEEVISRFAKDGIPLLDPEEDMEVKSSSYRKATRRIEALESLFEKHDVRNSPHIQQRLKIFHAKKEISAKIKSIKKTMRASTALAFKDELKARKRVLRRLGYITSEDVVEVKGKVACEISSADELTLTELMFSGALKDATVEQMVALLSCFVWQEKLQDAPKPRDELDLLFYQLQETARRVANLQLECKIQIDVETFVNSFRPDVMEAVYSWARGSKFHQIMEMTQVFEGSLIRAIRRLEEVLQQLILASKSIGETELEAKLEEAVSKIKRDIVFAASLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1H346</accession>
    <name>I1H346_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100838118</name>
        <name evidence="11" type="ORF">BRADI_1g55560v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ELIP/psbS family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557495.1">
        <property type="nucleotide sequence ID" value="XM_003557447.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H346"/>
    <dbReference type="STRING" id="15368.I1H346"/>
    <dbReference type="EnsemblPlants" id="KQK20603">
        <property type="protein sequence ID" value="KQK20603"/>
        <property type="gene ID" value="BRADI_1g55560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838118"/>
    <dbReference type="Gramene" id="KQK20603">
        <property type="protein sequence ID" value="KQK20603"/>
        <property type="gene ID" value="BRADI_1g55560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838118"/>
    <dbReference type="eggNOG" id="ENOG502RZBJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099630_0_0_1"/>
    <dbReference type="OMA" id="VWPKDTE"/>
    <dbReference type="OrthoDB" id="765963at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="18"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK20603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="188" mass="19029" checksum="74A4E37F7A0792F6" modified="2012-06-13" version="1">MATMMALSSFAGAAAVGRSANRSALAPRRRSLVVRAQTEPEMDPTKETTTSASASSPTPSPSPAAPKPKAKASPSVWDALAFSGPAPERINGRLAMVGFVAALSVEAARGGGLLSQAGSGAGLGWFLTTTALLSVASLVPLLQGQSVESKSSGFWTADAELWNGRFAMLGLVALAATEVITGAPFVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1H3C0</accession>
    <name>I1H3C0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100822781</name>
        <name evidence="3" type="ORF">BRADI_1g56160v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557528.1">
        <property type="nucleotide sequence ID" value="XM_003557480.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H3C0"/>
    <dbReference type="STRING" id="15368.I1H3C0"/>
    <dbReference type="EnsemblPlants" id="KQK20706">
        <property type="protein sequence ID" value="KQK20706"/>
        <property type="gene ID" value="BRADI_1g56160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822781"/>
    <dbReference type="Gramene" id="KQK20706">
        <property type="protein sequence ID" value="KQK20706"/>
        <property type="gene ID" value="BRADI_1g56160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822781"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_1"/>
    <dbReference type="OMA" id="KDANTMF"/>
    <dbReference type="OrthoDB" id="1669814at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107">
        <property type="entry name" value="NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107:SF7">
        <property type="entry name" value="RE15974P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK20706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="282" mass="28217" checksum="B311E27A84A12E5D" modified="2012-06-13" version="1">MATAADTAAAAKAADAVAAVASRDHPACSQPLAGRVAIVTGASRGIGRAIATHLSSLGASLVLGYASSASLAEQLAASLLPPNSAIAVKADVSSEAGVRSLFDAAESAFGRPAQILVANAGVLDDKYPSLAATATADFDRVFSTNARGAFLCLREAANRIPPGCGGGGGGRIVAVTSSVVASLPEGYAAYTASKAAVEAMVRTMAKELRGTRVTANCVAPGATATDMFFAGKSEETVRRVAEGNPMCRIGEAGDIAPVVGFLCTDAAEWINGQVIRANGGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HA05</accession>
    <name>I1HA05_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MsrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100826003</name>
        <name evidence="1" type="ORF">BRADI_1g76000v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562125.1">
        <property type="nucleotide sequence ID" value="XM_003562077.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HA05"/>
    <dbReference type="STRING" id="15368.I1HA05"/>
    <dbReference type="EnsemblPlants" id="KQK23770">
        <property type="protein sequence ID" value="KQK23770"/>
        <property type="gene ID" value="BRADI_1g76000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826003"/>
    <dbReference type="Gramene" id="KQK23770">
        <property type="protein sequence ID" value="KQK23770"/>
        <property type="gene ID" value="BRADI_1g76000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826003"/>
    <dbReference type="eggNOG" id="ENOG502RZ7Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150959_0_0_1"/>
    <dbReference type="OMA" id="RTKIICN"/>
    <dbReference type="OrthoDB" id="2015559at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045282">
        <property type="entry name" value="At4g08330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33674">
        <property type="entry name" value="METHIONINE-S-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33674:SF5">
        <property type="entry name" value="METHIONINE-S-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24046">
        <property type="entry name" value="At4g08330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK23770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="15502" checksum="01AACB85E4474B27" modified="2012-06-13" version="1">MAQAPAPAPATTSYGCAACGADLNLSSAHLYPAGVYFEAGNKGTLSFSWADESRLRLAAEDRIRPFFETLDYWGIQRKRTRISCDACGRLLGHVYDDGPPVMEGTGQFGMGPSQVVPRRPRYRFKIKAVTPIAGGGAASSAEAASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HEH2</accession>
    <name>I1HEH2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Amino acid permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100846498</name>
        <name evidence="9" type="ORF">BRADI_2g10510v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314869.1">
        <property type="nucleotide sequence ID" value="XM_024459101.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314870.1">
        <property type="nucleotide sequence ID" value="XM_024459102.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314871.1">
        <property type="nucleotide sequence ID" value="XM_024459103.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314872.1">
        <property type="nucleotide sequence ID" value="XM_024459104.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314873.1">
        <property type="nucleotide sequence ID" value="XM_024459105.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314874.1">
        <property type="nucleotide sequence ID" value="XM_024459106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HEH2"/>
    <dbReference type="EnsemblPlants" id="KQK03895">
        <property type="protein sequence ID" value="KQK03895"/>
        <property type="gene ID" value="BRADI_2g10510v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846498"/>
    <dbReference type="Gramene" id="KQK03895">
        <property type="protein sequence ID" value="KQK03895"/>
        <property type="gene ID" value="BRADI_2g10510v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_0_2_1"/>
    <dbReference type="OMA" id="TYCIAMF"/>
    <dbReference type="OrthoDB" id="3257095at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015185">
        <property type="term" value="F:gamma-aminobutyric acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015180">
        <property type="term" value="F:L-alanine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015812">
        <property type="term" value="P:gamma-aminobutyric acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000026">
        <property type="entry name" value="Amino-acid permease BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF32">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK03895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="55593" checksum="DB66C1ADED4BB0FF" modified="2012-06-13" version="1">MAVSGSGAAVDTGDADADQARLHQLGYKQELKRGLSAMSNFAFSFSIISVMAGVTTTYNAGLRYGGPASMTLGWLVVAAFNGCVALSMAEICSAYPTSGGLYYWSAKLAGNKWAPLASWITGWFNIVGQWALTTSTDFSLAQLVQVIILLGTGGANGGGYMASKYVVLAIHGFFLIMHGLINSLPIRWLSWFGHLGAFWNTVGAFVLVILIPAVAKEKASTEFIFTHFNTDNGMRIHGKSYILALGLLTSQYSLLGYDASAHMIEETKNADWSGPIGIITSVALSTMFGWIFLVALTSIVTDIPYLLSPDNDAGGYAVAQALYTSFDKRYGSGVGGLVCVGIVAVGIFLAGVACIASNSRMGYAFSRDRAMPFSHVWHRVSQNEVPLNVVWLSVVVAFVMALTSLGSQVAFQAMVSIATLGQYIAYALPIFFRVTTARRSFVPGPFYLGRYGVAVGWAAVLWVAFLTVLFSLPVAYPVAKDNFNYTPVAVGGVLLLSMVAWVVHARFWFQGPITNVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1HFM1</accession>
    <name>I1HFM1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100831236</name>
        <name evidence="14" type="ORF">BRADI_2g13790v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567755.1">
        <property type="nucleotide sequence ID" value="XM_003567707.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HFM1"/>
    <dbReference type="STRING" id="15368.I1HFM1"/>
    <dbReference type="EnsemblPlants" id="KQK04494">
        <property type="protein sequence ID" value="KQK04494"/>
        <property type="gene ID" value="BRADI_2g13790v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831236"/>
    <dbReference type="Gramene" id="KQK04494">
        <property type="protein sequence ID" value="KQK04494"/>
        <property type="gene ID" value="BRADI_2g13790v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831236"/>
    <dbReference type="eggNOG" id="ENOG502QT84">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_92_6_1"/>
    <dbReference type="OMA" id="GSNMEPY"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000467">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000275">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000722">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF83">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094542" description="Protein kinase domain-containing protein" evidence="12">
        <location>
            <begin position="19"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="391"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK04494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="694" mass="74814" checksum="F617977EC28D5E03" modified="2012-06-13" version="1">MATVLSLVLLLLPVATDALTADGQALLAFKAAVLRDPTGALADWNNSTDDPCSWNGVACDRGTRRVVALSLPRKGLVAALPASALPDSLRHLNLRSNRLFGALPPALVAGAVGLQSLVLSGNQLYGLVPRELGDLPYLQILDLSSNSLNGSLPGSILKCRRLRTLALGHNNLRGPLPPGFGRELSALERLDLSYNRFSGGIPEDIGNLSRLEGTVDLSHNDFSGLIPATLGKLPEKVYIDLTFNNLSGPIPQNGALENRGPTAFMGNPGLCGPPLKNPCSPDAMPSSKPGESAPASSGGKGLGKVAIVAIVLSDVVGILIIALVFLYCYRRTVFPREKGQGGAAGSKGSRSGKDCGCFRRDESETALDQEEQYDLVVLDRQVRFDLDELLKASALVLGKSGIGIVYKVVLEDGLSMAVRRLGEGGLQRFKEFQTEVDAIGKVRHPNIVTLKAYYWSSDEKLLIYDYISNGSLSAAIHGKPESMTFSPLPWDARLKIMKGVASGMSFLHEFSPKKYVHGDLRPNNVLLGTGMEPYISDFGLGRLANIAGGGSPFAESDRDGLEKAQIQHPDASVCPILSKGPCYQAPEALITLKPSQKWDVYSYGVILLEIITGRSPVVLLETMQMDLVQWVQFCIEEKKESADVLDPFLARESEREDEMIAVLKIALACIQANPERRPSMRHVTQTLERLNVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJG1</accession>
    <name>I1HJG1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100841423</name>
        <name evidence="7" type="ORF">BRADI_2g25430v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753912.2">
        <property type="nucleotide sequence ID" value="XM_014898426.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJG1"/>
    <dbReference type="EnsemblPlants" id="KQK06271">
        <property type="protein sequence ID" value="KQK06271"/>
        <property type="gene ID" value="BRADI_2g25430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841423"/>
    <dbReference type="Gramene" id="KQK06271">
        <property type="protein sequence ID" value="KQK06271"/>
        <property type="gene ID" value="BRADI_2g25430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841423"/>
    <dbReference type="eggNOG" id="KOG1237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009313_4_2_1"/>
    <dbReference type="OMA" id="LSECFCN"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="501"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="541"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK06271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="65743" checksum="647AA89594CD436F" modified="2012-06-13" version="1">MAGAAPADEDGSPKKTKQGGFKTMPFILANEICDRFATAGFNANLITYLTQQLHLPLVEASNLLTNFMGTAAFTPVFGAIIADSFTGRFWAIAGGGLLYQLGMLGLVVSALTPSLRPGPCTAPPGTSCQRANGGQLAMLYLSLLFTALGSGGIRPCVVAFGADQFGVGGRRPGGGQKWSFFNFYFFTMGLAVLLALTVVVYIQENVGWGWGFGIPAMAMFVSVLSFVAGYPLYVRVKPGGSPFVRLVQVVVAAVRKRNEAVPEDAALLYQNKELDAPIAVDGRLLHTNQLRFLDRAAIVTTTDAVVVDSSTGAEPEPDPWRLSTVHRVEELKSIVRMLPLWAASITLIAAASHNFTFAIQQSRTMDRRLSSHFEIPPASMIIFTTLTMLLSLSLYDRAFVPLARRYTGLPSGITYFQRMGAGLAVSVLGVLAGALVERKRRAVAAEHGLLDGGAAAAVPMSVFWLVPQYALHGVSDTLSTVAHMEFLYDQSPESMRSSAAALFWVAGSLGNYLGTALVTVVQSASGGEWLQDNINRGRLDYYYWLVTFLMALNLVYYIVCFHFYTLKSFEVDAGDKARRPEDGADQGPSIEPVTALSAGQIGGSRHCEGPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQC0</accession>
    <name>I1HQC0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100834918</name>
        <name evidence="13" type="ORF">BRADI_2g46420v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the Aux/IAA family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569553.1">
        <property type="nucleotide sequence ID" value="XM_003569505.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQC0"/>
    <dbReference type="FunCoup" id="I1HQC0">
        <property type="interactions" value="271"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HQC0"/>
    <dbReference type="EnsemblPlants" id="KQK09168">
        <property type="protein sequence ID" value="KQK09168"/>
        <property type="gene ID" value="BRADI_2g46420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834918"/>
    <dbReference type="Gramene" id="KQK09168">
        <property type="protein sequence ID" value="KQK09168"/>
        <property type="gene ID" value="BRADI_2g46420v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834918"/>
    <dbReference type="eggNOG" id="ENOG502R3EF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049393_1_0_1"/>
    <dbReference type="OMA" id="XARAGEG"/>
    <dbReference type="OrthoDB" id="673488at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HQC0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000078">
        <property type="entry name" value="Auxin-responsive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF16">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="151"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="76"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK09168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="271" mass="28333" checksum="3C34ED690911C6A5" modified="2012-06-13" version="1">MSPPLEPHDYIGLSAAAASPSPTSSSSCSSSANPGAEVGPRLTLRLGLPGSESPDRDSSADVAAALTLGLVSTHKAASKRAFPEASPRRGGSAAAAAGARGEEKAPAAAPPAAKAQVVGWPPVRNYRKNTLAASVSKSKGGEEGAAAQGGPQYVKVSMDGAPYLRKVDLKIYSSYEDLSTALQKMFSCFITGQSAMRKPSSKDRLTNGSKADSLQDQEYVLTYEDKDADWMLVGDLPWDLFTTICRKLKIMRGSDAAGIAPRSLEQTGQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQS0</accession>
    <name>I1HQS0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100846295</name>
        <name evidence="18" type="ORF">BRADI_2g47670v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232203.1">
        <property type="nucleotide sequence ID" value="XM_010233901.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQS0"/>
    <dbReference type="STRING" id="15368.I1HQS0"/>
    <dbReference type="EnsemblPlants" id="KQK09386">
        <property type="protein sequence ID" value="KQK09386"/>
        <property type="gene ID" value="BRADI_2g47670v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09387">
        <property type="protein sequence ID" value="KQK09387"/>
        <property type="gene ID" value="BRADI_2g47670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846295"/>
    <dbReference type="Gramene" id="KQK09386">
        <property type="protein sequence ID" value="KQK09386"/>
        <property type="gene ID" value="BRADI_2g47670v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09387">
        <property type="protein sequence ID" value="KQK09387"/>
        <property type="gene ID" value="BRADI_2g47670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846295"/>
    <dbReference type="eggNOG" id="KOG0475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015263_3_0_1"/>
    <dbReference type="OMA" id="CTALSME"/>
    <dbReference type="OrthoDB" id="4564at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902476">
        <property type="term" value="P:chloride transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00400">
        <property type="entry name" value="Voltage_gated_ClC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000008">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050368">
        <property type="entry name" value="ClC-type_chloride_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0129">CBS domain</keyword>
    <keyword evidence="12 15" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="445"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="476"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="17">
        <location>
            <begin position="568"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="17">
        <location>
            <begin position="641"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="501"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="504"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="514"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK09387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="709" mass="75282" checksum="AE4F2B48907F0DE0" modified="2012-06-13" version="1">MAPSATPCSLRLLLASSASPPPLFCAASVRPLLTGNRGGRVHLRIRCGAADEETGARRGEQVKASVEEMAPGRDLVTLAACLVGLLTGVSVVLFNLSVHEIRDLLWDGIPLRGASWLREEPTGEIWQRVIFVPVSGGIIVGGLNALRNSIKSNSDGTVSRIMGVFRPFLKAAAASFTLGTGNSLGPEGPSVEIGSAIAKGFGNVFRWEDGKTMSLVAAGSAAGIASGFNAAVAGCFFAVESVLWPSSVNSSSLSNSTPMVILSAVIASVVSEIGLGSDPAFTVPEYDFRSPTELPLYLLLGVFCGLVSITLSRCTALTMDIVENLQKTAGLPMAASPALGGLIVGLLALIYPEVLYWGFENVDILLESRPFTSGLSATVLVQLIGVKILATSLCRAFGLVGGYYAPSLFIGAATGMAYGKFMRFTFTGPESLFHVPFLDVASPQAYGLVGMAATLAGVCKVPLTSVLLLFELTQDYRIVLPLLGAVGLSSWIASPQRFSKSSKGKLDSLEEKTSTVQQTKSVPSQTQQVNSLDSVDATAELCKLESSLCLYNVRDENILENLTVAEAMKTRYISVSLTTPIIEALNLMFVEKQPFVVIIESNKSLVGLLTLKDFQDFCGTVKITKAQTEVHEHLVSHVCGVIKCKMCSVTPQMPLATAEKIMDSHGVDQLPVISANHQDGGPLIGFIDRECITIARRASAAKDFFKFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQZ2</accession>
    <name>I1HQZ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100824390</name>
        <name evidence="2" type="ORF">BRADI_2g48240v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569687.1">
        <property type="nucleotide sequence ID" value="XM_003569639.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQZ2"/>
    <dbReference type="STRING" id="15368.I1HQZ2"/>
    <dbReference type="EnsemblPlants" id="KQK09482">
        <property type="protein sequence ID" value="KQK09482"/>
        <property type="gene ID" value="BRADI_2g48240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824390"/>
    <dbReference type="Gramene" id="KQK09482">
        <property type="protein sequence ID" value="KQK09482"/>
        <property type="gene ID" value="BRADI_2g48240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824390"/>
    <dbReference type="eggNOG" id="KOG3010">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049344_5_0_1"/>
    <dbReference type="OMA" id="STHAYEM"/>
    <dbReference type="OrthoDB" id="10027013at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44575">
        <property type="entry name" value="OS01G0589200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44575:SF1">
        <property type="entry name" value="OS01G0716500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 12" evidence="1">
        <location>
            <begin position="39"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK09482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="261" mass="28958" checksum="457B808C75D31A7B" modified="2012-06-13" version="1">MADLYEKPSETYAKKRPRYPKEWFSMLASLTAGHHRAWDAGCGSGQASVSIAEHYDSVVATDVSEAQLRHGIAHPRVRYLHTPEGLTEDGLVALVGGERSLDLVIVATAIHWFDVPLFYAVVSRALKKPGGVLAVWGYNYDIRPFEDKLQGRLYAAARAYMDPRTRLAMERYRGLPFPFEPVGVGREGEPADVDMEVEMTLDDLAGFVMTGSVATTAREQGVDLEALVKGVMKEVEEEWGDQPTVPRKLVFKAFMLAGKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HV92</accession>
    <name>I1HV92_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100836127</name>
        <name evidence="11" type="ORF">BRADI_2g60910v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the villin/gelsolin family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK11568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003565032.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HV92"/>
    <dbReference type="STRING" id="15368.I1HV92"/>
    <dbReference type="EnsemblPlants" id="KQK11568">
        <property type="protein sequence ID" value="KQK11568"/>
        <property type="gene ID" value="BRADI_2g60910v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK11569">
        <property type="protein sequence ID" value="KQK11569"/>
        <property type="gene ID" value="BRADI_2g60910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836127"/>
    <dbReference type="Gramene" id="KQK11568">
        <property type="protein sequence ID" value="KQK11568"/>
        <property type="gene ID" value="BRADI_2g60910v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11569">
        <property type="protein sequence ID" value="KQK11569"/>
        <property type="gene ID" value="BRADI_2g60910v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836127"/>
    <dbReference type="eggNOG" id="KOG0443">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002568_2_0_1"/>
    <dbReference type="OMA" id="WKGRGAN"/>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11293">
        <property type="entry name" value="gelsolin_S4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11291">
        <property type="entry name" value="gelsolin_S6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000002">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000039">
        <property type="entry name" value="Villin-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000028">
        <property type="entry name" value="Villin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF51">
        <property type="entry name" value="PROTEIN FLIGHTLESS-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0117">Actin capping</keyword>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HP" evidence="10">
        <location>
            <begin position="916"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="771"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="863"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="772"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="798"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="897"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK11568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="981" mass="107726" checksum="00440C080DE70B8D" modified="2012-06-13" version="1">MSTAKVLDPAFQGAGQKVGIEIWRIEDFKPVPLPKSDYGKFYCGDSYIVLQTTSPKGGAYLYDIHFWIGKDSSQDEAGTAAIKTVELDSILGGRAVQHRELQGYESDKFLSYFKPCIIPMEGGFASGFKTPEEDKFETRLYICKGRRAIRIKEVPFARSSLNHDDVFILDTESKIYQFNGANSNIQERAKSLEVIQHLKEKYHGGVCDVAIVDDGKLQAESDSGEFWVLFGGFAPIGKKTVSDDDVVLETTPPKLYSINDGQLKLEDTALTKAVLENTRCFLLDCGAEMFVWVGRVTQLDDRKATTKAVEEFIIDQKRPKTTRVTQVIQGYESHAFKSKFESWPVGNVAGNSGAEDGRGKVAALLKQQGVDVKGAAKSSTPINEEVPPLLEGGGKLEVWCVDGSAKTVLPKEDNGKFYSGDCYIVLYTYHSGDKKEEYYLNYWIGKDSTTDDQAMAAELANTMWNSLKGRPVLGRIFQGKEPPQFVALFQPMVILKGGIGSGYKKIAEEKGVGSGMYSAEGIALFRVSGTAIHNNKTLQVDAKATSLSSTDCFVLQSGSAMFTWHGNSSTYEQQQWAAKVAEFLKPGATVKHSKEGTESSAFWFALDGKQSYTNKTVTQDIIVREPHLYAFSFRKGRLEVTEIFNFCQDDLLTEDMMILDTHGEVFIWIGQCVESKEKHKAFDIGQKYIEHAMSIEDLSAYVPLYKVSEGNEPSFFKTYFSWDNTKSVVHGNSFQKKLSLLFGLRSESTSRSSGNGGPTQRASALAALSSAFNPSSQQKQANDSRPASSGDGGPTQRASALAALSNAFNPSSKPKTPPPSRSGQGSQRAAAVAALSSVLTAEQSGSSDNLRASKMSTTAEKIDADVAVITPSEASPRSEAGESSEFQSEKDAVVDEVPSEGDGAEPEAPEEQTTEHVGEVTFSYDRLISKSADPIRGIDYKRREAYLSESEFQTVFGVTKDAFYQQPAWKQELQKRKADLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1I0E6</accession>
    <name>I1I0E6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100842759</name>
        <name evidence="4" type="ORF">BRADI_3g13467v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ94825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571304.1">
        <property type="nucleotide sequence ID" value="XM_003571256.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I0E6"/>
    <dbReference type="STRING" id="15368.I1I0E6"/>
    <dbReference type="EnsemblPlants" id="KQJ94825">
        <property type="protein sequence ID" value="KQJ94825"/>
        <property type="gene ID" value="BRADI_3g13467v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842759"/>
    <dbReference type="Gramene" id="KQJ94825">
        <property type="protein sequence ID" value="KQJ94825"/>
        <property type="gene ID" value="BRADI_3g13467v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842759"/>
    <dbReference type="eggNOG" id="KOG2308">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006932_1_0_1"/>
    <dbReference type="OMA" id="WQNENIV"/>
    <dbReference type="OrthoDB" id="431378at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057826">
        <property type="entry name" value="WWE_C20G8.02"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF10">
        <property type="entry name" value="LD21067P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23463">
        <property type="entry name" value="WWE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDHD" evidence="3">
        <location>
            <begin position="674"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="489"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="597"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ94825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="934" mass="104720" checksum="2EE34FB413CA381B" modified="2012-06-13" version="1">MESPEASSARGAPPGPDGASTSHAAEGASPDSLQNTPSNIARLEDAIENCAARRKYLARTKSASDGEDVRWYFCKLPLADRVLSASVPRTEIVGKGDYFRFSMRDCLALEAAFLEREESLLGYWWREYAECSEGPTGSLAKTDSSDSEYLYKVEEERVGVPVKGGLYEVDLMRRHCFPVYWNGENRRVLRGNWFARKGGLDWIPLREDVSEQLELAYNCQVWHRRKFQPSGLFAARVDLQGSTPGLHALFTGEDDTWEAWLVFDTGPKLGSNTIKLRRGFSSSGSANPTQDELRQQKEEETDDYCSQVPVGHLVFMVHGIGQRLEKANLVDDVVDFRRVTANLADRYLTPYQRSTQRVLYIPCQWRKSLKLGGERTVEKITLDGVKGLRVALGATVHDVLYYMSPIYCQHIIDSVSSQLNQLYMKFLKRNPGYSGKVSLYGHSLGSVLTYDILCHQESLSAPFPTDYLNMEISSDEGHQAPNTFTIHNSGVKEHDTSSTSGRSCADNVNGVDEDGTRNDHSLTDNIIPSCVPESVPNNDDTLAPPISVDGLQTEVENQNQAENHQMAYTEEGATSGVCTKDADQWISRSAEEQHEAVLDKDKSISSLEEEVRRLKAKLAELEQQKDLVTQNISSVGSHQDKDVNCTVSLASGKLDVGQGSTSQSYRPRIRYTKLNFKVDTFFAVGSPLGVFLSLRNVRIGVGRGQDYWQDKNIVEEMPCCRQMFNVFHPFDPVAYRIEPLVCEDYVNKRPVIIPYHRGGKRIHVGVQEFTEDVAARSQSIARQFKSLKVKAVAALLSLSKNDTEDDGEKTEEEERSYGSMMMERLTGSPDGRVDHVLQEKTFQHPYLSALGSHTNYWRDHDTALFIIKHLYRDIPEEPPTDAAGGTPIRLFYVRDPIAEDTPLTFSDNSSVKEFSRKMKTYSRETLNDANCGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1I0X7</accession>
    <name>I1I0X7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100846310</name>
        <name evidence="16" type="ORF">BRADI_3g14980v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Interacts with ATG4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234307.1">
        <property type="nucleotide sequence ID" value="XM_010236005.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757206.1">
        <property type="nucleotide sequence ID" value="XM_014901720.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I0X7"/>
    <dbReference type="SMR" id="I1I0X7"/>
    <dbReference type="STRING" id="15368.I1I0X7"/>
    <dbReference type="EnsemblPlants" id="KQJ95062">
        <property type="protein sequence ID" value="KQJ95062"/>
        <property type="gene ID" value="BRADI_3g14980v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66624">
        <property type="protein sequence ID" value="PNT66624"/>
        <property type="gene ID" value="BRADI_3g14980v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846310"/>
    <dbReference type="Gramene" id="KQJ95062">
        <property type="protein sequence ID" value="KQJ95062"/>
        <property type="gene ID" value="BRADI_3g14980v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66624">
        <property type="protein sequence ID" value="PNT66624"/>
        <property type="gene ID" value="BRADI_3g14980v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846310"/>
    <dbReference type="eggNOG" id="KOG1654">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119276_0_1_1"/>
    <dbReference type="OMA" id="AKMKWMF"/>
    <dbReference type="OrthoDB" id="6738456at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008429">
        <property type="term" value="F:phosphatidylethanolamine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16128">
        <property type="entry name" value="Ubl_ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000010">
        <property type="entry name" value="Autophagy-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0072">Autophagy</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="13">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQJ95062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="119" mass="13789" checksum="F3D374D25F495100" modified="2012-06-13" version="1">MARSSFKLEHPLERRQAEANRIREKYPDRIPVIVEKAERSDIPDIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNTLPPTAALMSAIYEENKDEDGFLYMTYSGENTFGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1J1</accession>
    <name>I1I1J1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100831253</name>
        <name evidence="4" type="ORF">BRADI_3g16860v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571477.1">
        <property type="nucleotide sequence ID" value="XM_003571429.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1J1"/>
    <dbReference type="EnsemblPlants" id="KQJ95380">
        <property type="protein sequence ID" value="KQJ95380"/>
        <property type="gene ID" value="BRADI_3g16860v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66774">
        <property type="protein sequence ID" value="PNT66774"/>
        <property type="gene ID" value="BRADI_3g16860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831253"/>
    <dbReference type="Gramene" id="KQJ95380">
        <property type="protein sequence ID" value="KQJ95380"/>
        <property type="gene ID" value="BRADI_3g16860v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66774">
        <property type="protein sequence ID" value="PNT66774"/>
        <property type="gene ID" value="BRADI_3g16860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831253"/>
    <dbReference type="eggNOG" id="ENOG502QQPF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_21_4_1"/>
    <dbReference type="OMA" id="HYNDQDI"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I1J1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001029">
        <property type="entry name" value="Os02g0194400 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000887">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001">
        <property type="entry name" value="OS01G0253100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001:SF538">
        <property type="entry name" value="OS02G0227500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="32"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ95380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="324" mass="36240" checksum="8074EF34DFF5003C" modified="2012-06-13" version="1">MNLFGFFRVSKKRDSHSLHYNDQDITNNLGDDDAMSNINGGTWNSALYRVPLPGTSSVVVVKKLQNRSGSPVDASLDNRCQSEVNLLGSIHHDNIIGLADCLRTNNFIVLIYNHKENGSLHRWLHPDLPQLAAQGLVLDWPTRRNIAIGTVEGLCYLHHGRNHPIVHHNINSASILLDTSLNPKIAGFDFARINLAGPDQPVPISELTAANMFGYTAPEYVTMVTIKVDVYSIGVVLLELVTGRLPNEPVLDGHLATWAQNNCERLMEKTGDFSNVVDMVIPDRARYIKEMAAMFRLGVDCTVKKPEERPTIREGLFRLRNRCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1I317</accession>
    <name>I1I317_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine carboxypeptidase-like 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100845311</name>
        <name evidence="7" type="ORF">BRADI_3g21350v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573745.1">
        <property type="nucleotide sequence ID" value="XM_003573697.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I317"/>
    <dbReference type="FunCoup" id="I1I317">
        <property type="interactions" value="463"/>
    </dbReference>
    <dbReference type="MEROPS" id="S10.018"/>
    <dbReference type="EnsemblPlants" id="KQJ96166">
        <property type="protein sequence ID" value="KQJ96166"/>
        <property type="gene ID" value="BRADI_3g21350v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845311"/>
    <dbReference type="Gramene" id="KQJ96166">
        <property type="protein sequence ID" value="KQJ96166"/>
        <property type="gene ID" value="BRADI_3g21350v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845311"/>
    <dbReference type="eggNOG" id="KOG1282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008523_0_1_1"/>
    <dbReference type="OMA" id="RTHLECA"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I317">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000001">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000072">
        <property type="entry name" value="Serine carboxypeptidase-like 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF203">
        <property type="entry name" value="OS04G0176400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095024" description="Serine carboxypeptidase-like 18" evidence="6">
        <location>
            <begin position="32"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ96166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="52889" checksum="9B0240B9E01EFF71" modified="2012-06-13" version="1">MAATTLPLRLRSLLLLLCQLLLLLHPFFSGATPWITTKSVSRLPGFSGGDLPFSLETGYVGLDDGVRLFYYFIQSERSPEEDPVLLWLTGGPGCSALSGLVYEIGPLAFDFDGYTGGLPTLLYKPAAWTKVSNIIFVDSPAGTGFSYDSTHNRTIPSDTIVIRQLHIFLQTWFDEHPQFLPNPLYIAGDSYSGLIIPSLAMKIAKGIESGDERLVNLKGTIAGNAFTDIKLDFNARLPFLHGMGIIPDELYEAARENCRGEYRSPSNAPCANSLQAVTDCIKDVNDVHVLEPRCPEYLDLTIFHKQLKTLQDHGRKRLMLESAVSSVCRNATYFLSELWTNDKAVRESLGIQKGTVPSWQRCDFHIPYIMEISSTVYDHLSLIMKGYRSMIYSGDHDSKVSFVGTQAWIRHLNLSVTDVWRPWHLDSQVVGFTRTYSDNLTYATVKGAGHTAPEYMPRECLAMIDRWLSGQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1I3Y9</accession>
    <name>I1I3Y9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="4">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100834813</name>
        <name evidence="18" type="ORF">BRADI_3g26660v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-prolyl-[collagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:18945"/>
            <dbReference type="Rhea" id="RHEA-COMP:11676"/>
            <dbReference type="Rhea" id="RHEA-COMP:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571778.1">
        <property type="nucleotide sequence ID" value="XM_003571730.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I3Y9"/>
    <dbReference type="FunCoup" id="I1I3Y9">
        <property type="interactions" value="517"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I3Y9"/>
    <dbReference type="EnsemblPlants" id="KQJ96682">
        <property type="protein sequence ID" value="KQJ96682"/>
        <property type="gene ID" value="BRADI_3g26660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834813"/>
    <dbReference type="Gramene" id="KQJ96682">
        <property type="protein sequence ID" value="KQJ96682"/>
        <property type="gene ID" value="BRADI_3g26660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834813"/>
    <dbReference type="eggNOG" id="KOG1591">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058132_5_0_1"/>
    <dbReference type="OMA" id="CAHCRIE"/>
    <dbReference type="OrthoDB" id="420380at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000002">
        <property type="entry name" value="Prolyl 4-hydroxylase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF238">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01549">
        <property type="entry name" value="ShK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00254">
        <property type="entry name" value="ShKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51670">
        <property type="entry name" value="SHKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095089" description="procollagen-proline 4-dioxygenase" evidence="15">
        <location>
            <begin position="21"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="16">
        <location>
            <begin position="118"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="ShKT" evidence="17">
        <location>
            <begin position="256"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQJ96682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="298" mass="32605" checksum="D5048DDDB143E0CC" modified="2012-06-13" version="1">MARILVAVLALHLTAAVASAASSVAGGGAFDPSRVVQLSWRPRAFLHKGFLSEPECDHMIELAKDKLEKSMVADNESGKSVQSEVRTSSGMFLEKRQDEVVARIEERIAAWTFLPSENGESIQILHYKNGEKYEPHYDYFHDKNNQALGGHRIATVLMYLSNVEKGGETIFPNAEGKLTQHKDETASECAKNGYAVKPMKGDALLFFSLHPDATTDPDSLHGSCPVIEGQKWSATKWIHVRSFENPGKQGASGDGCEDENVLCAQWAAVGECAKNPNYMVGTKEAPGFCRKSCNLCAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5A2</accession>
    <name>I1I5A2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Salt tolerance receptor-like cytoplasmic kinase 1</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Receptor-like cytoplasmic kinase 154</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC100823795</name>
        <name evidence="24" type="ORF">BRADI_3g30490v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">Bd21</strain>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Acts probably as a dual specificity protein kinase. Regulates hydrogen peroxide (H(2)O(2)) homeostasis and improves salt tolerance by phosphorylating tyrosine residues of CATC thus activating its catalase activity. Promotes growth at the seedling stage and prevents grain yield loss under salt stress conditions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Self-interacts. Interacts with CATA, CATB and CATC at the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574174.1">
        <property type="nucleotide sequence ID" value="XM_003574126.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5A2"/>
    <dbReference type="STRING" id="15368.I1I5A2"/>
    <dbReference type="EnsemblPlants" id="KQJ97394">
        <property type="protein sequence ID" value="KQJ97394"/>
        <property type="gene ID" value="BRADI_3g30490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823795"/>
    <dbReference type="Gramene" id="KQJ97394">
        <property type="protein sequence ID" value="KQJ97394"/>
        <property type="gene ID" value="BRADI_3g30490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823795"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_21_4_1"/>
    <dbReference type="OMA" id="YLANWSG"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902074">
        <property type="term" value="P:response to salt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000514">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000419">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001">
        <property type="entry name" value="OS01G0253100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001:SF543">
        <property type="entry name" value="OS10G0516200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="59"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="22">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00072135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQJ97394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="353" mass="37172" checksum="5188F94F859BA314" modified="2012-06-13" version="1">MGQRYRFFCCGCGANAAADDDREADEEGGAGVPDGGKGGDGVVAARQLSWAQVERMTGGFTSAVVGEGGFSTVYLARLSGALAAAKVHRSSERLHRVFRQELETLLRVRHPHVVRLLAFCEQQDEGVLVLEFAANGNLHEKLHGGGKAAGTMPWARRVSVALQVAQALEYLHDRCEPQVVHGDVKASNVLLDSAMSAKLCDFGSARMGFSAAVRPRAHQTMLGSPGYVDPHYIRSGVVTKKTDVYSFGVLLLELLTGMEAFCPVEGRLLTAVLAPRLKPAGGVPCDARALVDERLGSAYDAAEASAVAALAASCVGQNPSLRPSMADVVRTLEQSAQGSILSVGKGSDGIGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1I690</accession>
    <name>I1I690_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC104584190</name>
        <name evidence="8" type="ORF">BRADI_3g33670v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236663.1">
        <property type="nucleotide sequence ID" value="XM_010238361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I690"/>
    <dbReference type="STRING" id="15368.I1I690"/>
    <dbReference type="EnsemblPlants" id="KQJ97845">
        <property type="protein sequence ID" value="KQJ97845"/>
        <property type="gene ID" value="BRADI_3g33670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584190"/>
    <dbReference type="Gramene" id="KQJ97845">
        <property type="protein sequence ID" value="KQJ97845"/>
        <property type="gene ID" value="BRADI_3g33670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584190"/>
    <dbReference type="HOGENOM" id="CLU_2029904_0_0_1"/>
    <dbReference type="OMA" id="MFETAHE"/>
    <dbReference type="OrthoDB" id="681852at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677">
        <property type="entry name" value="AP2 DOMAIN CLASS TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677:SF75">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF084"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="25"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ97845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="122" mass="13239" checksum="2249BC5DEAE5BA73" modified="2012-06-13" version="1">MVVTDEAAAGRRKKKTRPAPEARTEFRGVCRQPSGRYRGQIKRSGTTLHLGIFDTAHEAARGYDAAAAQLHGAKAITNFKRRQSPAPTAAAAHNDGEAMDFSGFPEVPVELRLLPYGILQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I1I727</accession>
    <name>I1I727_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-haem dioxygenase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g35920v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I727"/>
    <dbReference type="EnsemblPlants" id="KQJ98282">
        <property type="protein sequence ID" value="KQJ98282"/>
        <property type="gene ID" value="BRADI_3g35920v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ98282">
        <property type="protein sequence ID" value="KQJ98282"/>
        <property type="gene ID" value="BRADI_3g35920v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2870670_0_0_1"/>
    <dbReference type="InParanoid" id="I1I727"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ98282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7266" checksum="59D00D8C2777E2E5" modified="2012-06-13" version="1">MPEIFALPLEAKQRNASTLGPFRGYIHNLPGMNWESLRLSDVNVAGSVRDFTDTFWPEGNPAFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEM2</accession>
    <name>I1IEM2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100846611</name>
        <name evidence="5" type="ORF">BRADI_3g57300v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570524.1">
        <property type="nucleotide sequence ID" value="XM_003570476.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEM2"/>
    <dbReference type="EnsemblPlants" id="KQK01645">
        <property type="protein sequence ID" value="KQK01645"/>
        <property type="gene ID" value="BRADI_3g57300v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846611"/>
    <dbReference type="Gramene" id="KQK01645">
        <property type="protein sequence ID" value="KQK01645"/>
        <property type="gene ID" value="BRADI_3g57300v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846611"/>
    <dbReference type="eggNOG" id="ENOG502QWKN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019284_1_0_1"/>
    <dbReference type="OMA" id="HVEEYFT"/>
    <dbReference type="OrthoDB" id="608866at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IEM2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47122">
        <property type="entry name" value="MYB-LIKE DNA-BINDING DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47122:SF4">
        <property type="entry name" value="TRF-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="506"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="3">
        <location>
            <begin position="514"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK01645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="627" mass="70242" checksum="263DAD481CAEACA5" modified="2012-06-13" version="1">MEVGAPQDARENKNEENEFEENGSSDPVVYQLVRVEGDGTLVPATEDEVLQFEHFVDEKVDLPSIEDVGRVEDFFANNCMFLKPDLEEGPCKLETTGKLQTQKLDADLEEDRLKPLDDSLDIPSKCTVVHDKQPEKLNTEQGYHNTVHLDNASTETTKSTVLNDSCSVEKEKADACSRHVNDSSTEPSVSGITSSVPDFSILRGEVFLDTLTIRELQEAFRATFGRQTTVKDKLWLKRRITMGLTHSCDVPSSGCVIKDYIVVGKDAKEELPTIEGILKVEVEATSLARDQVLDHANERDTPSSSYYQSEDQQRSSKRLKGISTDNDESLGTLQDEQGTAKRLRKPTKRYIEELSDIETFDSTGKLSSPGKRPAHDEVLLRPRATFQEVDSLTITYPTRKDTFGGFSVHVPYVSRMRRGRPRRNFSSFLDDKPSVECAEVQMAVEMTVEKDGEQGNHVNQAMEVPLMNNAEKNGGHVETADNKEVRTVEANILCRADAKSKTKRSLKRKHHRAWTLSEVLKLVDGVARFGVGKWSEIRRLAFASYSYRTSVDLKDKWRNLIRASQTQLYLEKDGSCLRKSNPSTIPIPPSILLRVKELAEMQPQVGDLAAPMQLNSRAAQGKGSGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IG80</accession>
    <name>I1IG80_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100828932</name>
        <name evidence="17" type="ORF">BRADI_4g01070v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sterol desaturase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576810.1">
        <property type="nucleotide sequence ID" value="XM_003576762.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757441.1">
        <property type="nucleotide sequence ID" value="XM_014901955.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IG80"/>
    <dbReference type="STRING" id="15368.I1IG80"/>
    <dbReference type="EnsemblPlants" id="KQJ85692">
        <property type="protein sequence ID" value="KQJ85692"/>
        <property type="gene ID" value="BRADI_4g01070v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ85693">
        <property type="protein sequence ID" value="KQJ85693"/>
        <property type="gene ID" value="BRADI_4g01070v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ85694">
        <property type="protein sequence ID" value="KQJ85694"/>
        <property type="gene ID" value="BRADI_4g01070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828932"/>
    <dbReference type="Gramene" id="KQJ85692">
        <property type="protein sequence ID" value="KQJ85692"/>
        <property type="gene ID" value="BRADI_4g01070v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ85693">
        <property type="protein sequence ID" value="KQJ85693"/>
        <property type="gene ID" value="BRADI_4g01070v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ85694">
        <property type="protein sequence ID" value="KQJ85694"/>
        <property type="gene ID" value="BRADI_4g01070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828932"/>
    <dbReference type="eggNOG" id="KOG0539">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034756_1_0_1"/>
    <dbReference type="OMA" id="HDYPKDK"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="16">
        <location>
            <begin position="87"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ85693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="237" mass="27385" checksum="B34E7AFB7E674FCE" modified="2012-06-13" version="1">MVAQAFTVDLDKPLVFQVGHLEEQYQDWVHQPIVSKEGPRFFANDVLEFLTRTKWWAVPLIWLPVVCWCLNTSIQMGHTYPEVALMVVVGMFIWTLIEYTLHRYLFHIDTKSYWTNTAHYLLHGCHHKHPMDGLRLVFPPTAAAILCYPFWNLVKLFTTTSTTPGVFGGGLLGYVIYDCTHYYLHHGQPSSDPAKHLKKYHLNHHFRIQNKGFGITSTLWDHVFGTLPSTKTVDKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1IHK9</accession>
    <name>I1IHK9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100828314</name>
        <name evidence="2" type="ORF">BRADI_4g04900v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575439.1">
        <property type="nucleotide sequence ID" value="XM_003575391.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IHK9"/>
    <dbReference type="EnsemblPlants" id="KQJ86355">
        <property type="protein sequence ID" value="KQJ86355"/>
        <property type="gene ID" value="BRADI_4g04900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828314"/>
    <dbReference type="Gramene" id="KQJ86355">
        <property type="protein sequence ID" value="KQJ86355"/>
        <property type="gene ID" value="BRADI_4g04900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828314"/>
    <dbReference type="HOGENOM" id="CLU_1236547_0_0_1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IHK9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33115">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33115:SF22">
        <property type="entry name" value="OS12G0449900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ86355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25235" checksum="0BB0A0D1488F4A33" modified="2012-06-13" version="1">MGYLSMAPSSSSVASSPCSRRTFGCSQSQPSSGLFADRHAILLSHRQRELLNVSVRPKQIQLLVCPAYLRPISARADEPSIIIGKNMLTDAVNKMKRRPKSDYRQQLMDALLMLKKLAADGNNRRAMRRSFGMLSIVMAPVSSDLHRNDHGAWSSLAAASLKLMWLLVDDTSPSQRASIMKHEMSRKEEEITRSMMGILNCDKCDEQLRMLAKKILAQVKKIHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>I1ILE8</accession>
    <name>I1ILE8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Retinoblastoma-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100844602</name>
        <name evidence="12" type="ORF">BRADI_4g17210v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Regulator of biological processes that recruits a histone deacetylase to control gene transcription. May play a role in the entry into mitosis, negatively regulating the cell proliferation. Formation of stable complexes with geminiviridae replication-associated proteins may create a cellular environment which favors viral DNA replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the retinoblastoma protein (RB) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576010.1">
        <property type="nucleotide sequence ID" value="XM_003575962.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ILE8"/>
    <dbReference type="FunCoup" id="I1ILE8">
        <property type="interactions" value="1241"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1ILE8"/>
    <dbReference type="EnsemblPlants" id="KQJ88348">
        <property type="protein sequence ID" value="KQJ88348"/>
        <property type="gene ID" value="BRADI_4g17210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844602"/>
    <dbReference type="Gramene" id="KQJ88348">
        <property type="protein sequence ID" value="KQJ88348"/>
        <property type="gene ID" value="BRADI_4g17210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844602"/>
    <dbReference type="eggNOG" id="KOG1010">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="DYIRFGW"/>
    <dbReference type="OrthoDB" id="844594at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ILE8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
        <property type="term" value="P:regulation of DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000030">
        <property type="entry name" value="Retinoblastoma-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000075">
        <property type="entry name" value="Retinoblastoma-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015030">
        <property type="entry name" value="RB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024599">
        <property type="entry name" value="RB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF22">
        <property type="entry name" value="RETINOBLASTOMA-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11934">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08934">
        <property type="entry name" value="Rb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01367">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein N-terminal" evidence="10">
        <location>
            <begin position="8"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="11">
        <location>
            <begin position="287"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="523"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="741"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="845"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="854"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ88348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="878" mass="97636" checksum="FEB4A5315AA848E4" modified="2012-06-13" version="1">MGPPPVESNQLRSKIDEVLRSRYGPDWENQPELKQLQSLVNLMTDASRFYRKAFNELFSSASTCQESESTANNLDYTCFGWLLFLTLRSKSPELFKDLVSCIHGLVAILAILLIHVPARFRSFTIEGSSHLIKQTERGVDLLASLCHNYHTSEDRLKEMMGKSHQAIEEYFSMKPLTASECKAETLDKIDTVGLKYFKHLLDEECFQSNLENLEKLSSTSSWEGELDLKMFLINNDNIVSAEDSSSCPKHVFETLVSPTKTIKNMLTVPTSPSSPVNGGSVKIMQMTPVTSAMTTSKWLREVISSSPEKPSSKLEQFLSSCNRDLTSDITKRVNIVLEAIFPTRSSGDWGGSIGLNCANAFDIPWAEARKSEASKLYYRVLEAICRAESHNTNVNNLTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEESLPRELKRHLNALEEQLLESMAWEKGSSLYNSLVVARPSLASEINRLGLLAEPMPSLDDIVAHQNIHSEGLPATPSKKRAAGPDDNDDPQSPKRLCKESRNTLADHNLLTPPPKQSHMVKAKWHPLQSTFASPTVSNPVGGNEKCAEVGVNIFFSKILKLAAIRIRNLCERLRHVEQTERVYNIFKQILDQQTTLFFNRHIDQLILCCLYGVAKVSQLTLTFKEIVTNYKREQQCKPEVFRSVFVGSTNRNGGLGSRHVDIIVFYNEVFVPAVKPFLVDLIPSGARAEDKKNPSSQIPGSPKPSPFSNLPDMSPKKVSSSHNVYVSPLRQTKKDALLSPSSRSFYACIGESTHAFQSPSKDLAAINNRLNYTSRRINTRINFDMVSDSVVAGSLGQPNGGPASSDPAAAFSPLSKKGKPGPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMV9</accession>
    <name>I1IMV9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_4g23560v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IMV9"/>
    <dbReference type="STRING" id="15368.I1IMV9"/>
    <dbReference type="EnsemblPlants" id="KQJ89113">
        <property type="protein sequence ID" value="KQJ89113"/>
        <property type="gene ID" value="BRADI_4g23560v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89113">
        <property type="protein sequence ID" value="KQJ89113"/>
        <property type="gene ID" value="BRADI_4g23560v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040256_0_0_1"/>
    <dbReference type="InParanoid" id="I1IMV9"/>
    <dbReference type="OMA" id="WSHYLEI"/>
    <dbReference type="OrthoDB" id="412781at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000065">
        <property type="entry name" value="Programmed cell death 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095413" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats domain-containing protein" evidence="5">
        <location>
            <begin position="30"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="6">
        <location>
            <begin position="71"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ89113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="325" mass="38376" checksum="6E6074BB325EE552" modified="2012-06-13" version="1">MKNHQKLLLMFLLLLFVVLLILEMQIVTSVQDIVERGALREQDISALEEKALQKDKPQTLDEFEMLVRSSPNSSFIWINYMAFLLDLADVEKARSVAERALKTINIREEEEKLNVWVAYFNLENECGCPREHAVNKIFQRAVQYCDPKKVYLALLGMYEITEQHELADELFERMTKRFKTSCKIWLRRIQFSLKQGRDVQYIKSIINRALLSLPQSKRIKFLSQTAILEFKCGVPEEGRSIFELLLREYPKRTDLWSVYIDQEVRLGDTDVIRALFDRVTWLSLPPKKMKFLFKKYLAYEKSHGDRERMELVMQKATEYSKISQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPW1</accession>
    <name>I1IPW1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100823108</name>
        <name evidence="9" type="ORF">BRADI_4g29540v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578071.1">
        <property type="nucleotide sequence ID" value="XM_003578023.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPW1"/>
    <dbReference type="EnsemblPlants" id="KQJ90118">
        <property type="protein sequence ID" value="KQJ90118"/>
        <property type="gene ID" value="BRADI_4g29540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823108"/>
    <dbReference type="Gramene" id="KQJ90118">
        <property type="protein sequence ID" value="KQJ90118"/>
        <property type="gene ID" value="BRADI_4g29540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823108"/>
    <dbReference type="eggNOG" id="ENOG502QPJU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006348_7_0_1"/>
    <dbReference type="OMA" id="QLCTPRI"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000285">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000392">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000491">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF79">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="8">
        <location>
            <begin position="53"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="296"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="341"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="149"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="161"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ90118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="557" mass="57751" checksum="9E73652F13BD56E2" modified="2012-06-13" version="1">MGATRPRRWRLPFQRSAPSSPSSKSFPAPYSPALSAAAAASEAEPKGEQQEEAAPPEFLCPILGALMADPVILPSGQTYERACLQACKELSFFPPGTGSGSDAMIPNSALKAAIGTWCARSGRAVPALPSEEAAREAVLRAMPPPAAAAAAEAKSVRTRRPPVAASPSNSSYSSPASAASTSPSTSSSEITPAEEDVAVKTAKVVIQAVDPLENEVVAKVMDADEDGVVAAAMAALREATRESAERRRALCTPRLLAALRRVLLIPRHASARVDATAVLVNLSLEPANKVRIVRAGAVPALVEVLRTGGSSVPAEARENAAGALFGLALHEENRAAIGVLGAVPPLLDLLTSTTQHPRARRDAGMALYYLSLAAVNQSKVARFPGAPKALLAAASDAAEPTPIRRLALMVICNVGACAEGRATLMDAGAVAAVAGILSDDTTRVAELEEWCVAAIYALSRGSLRFRGLAHAAGADKALRRVADEGAPGGVRREMARKTLRAMRTDLDNDADADLTGSSLECGDGDDCGGSIVSDGLMSFRRRQRDLGASSCGNTTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IS25</accession>
    <name>I1IS25_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HpcH/HpaI aldolase/citrate lyase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100844725</name>
        <name evidence="6" type="ORF">BRADI_4g35820v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HpcH/HpaI aldolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578481.1">
        <property type="nucleotide sequence ID" value="XM_003578433.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IS25"/>
    <dbReference type="EnsemblPlants" id="KQJ91137">
        <property type="protein sequence ID" value="KQJ91137"/>
        <property type="gene ID" value="BRADI_4g35820v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844725"/>
    <dbReference type="Gramene" id="KQJ91137">
        <property type="protein sequence ID" value="KQJ91137"/>
        <property type="gene ID" value="BRADI_4g35820v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844725"/>
    <dbReference type="eggNOG" id="ENOG502QR7H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059964_0_0_1"/>
    <dbReference type="OMA" id="SRCEDET"/>
    <dbReference type="OrthoDB" id="1621678at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IS25">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000028">
        <property type="entry name" value="2-keto-3-deoxy-L-rhamnonate aldolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050251">
        <property type="entry name" value="HpcH-HpaI_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30502">
        <property type="entry name" value="2-KETO-3-DEOXY-L-RHAMNONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30502:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH/HpaI aldolase/citrate lyase" evidence="5">
        <location>
            <begin position="72"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ91137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="342" mass="36208" checksum="EF998D462E35ED49" modified="2012-06-13" version="1">MAVSATATSLSHLLPAPKPKPRTPPRLSLLPSNRKPSRAATSAIFAAAAAASDFLSPVPSLKSRLAAGDTLYGLFLLSFSPTLAEIAALAGYDYVVVDMEHGPGSITEALACLRALDAARTPAVLRLPEACPVWAKKALDLGPAGLMLPAIESPAAAAEAVSHCRYPPRGVRGAAYPIVRASAYGLDDSYVSRCEDDTLIICQVETAAGVAEVDAIAAVDGVDVVQMGPLDLSASMGYLWDPGNRKVRAALREAERKVLEARKKKVTASAGNAAYLGGFAMPNDPPEQLKMRGYHMVAGAVDIGLFRKAALEDVKWFKEAVMEIGEEEGEEEDEKDDGYWSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1IS28</accession>
    <name>I1IS28_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104584710</name>
        <name evidence="3" type="ORF">BRADI_4g35840v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238409.1">
        <property type="nucleotide sequence ID" value="XM_010240107.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318992.1">
        <property type="nucleotide sequence ID" value="XM_024463224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IS28"/>
    <dbReference type="EnsemblPlants" id="KQJ91139">
        <property type="protein sequence ID" value="KQJ91139"/>
        <property type="gene ID" value="BRADI_4g35840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584710"/>
    <dbReference type="Gramene" id="KQJ91139">
        <property type="protein sequence ID" value="KQJ91139"/>
        <property type="gene ID" value="BRADI_4g35840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584710"/>
    <dbReference type="eggNOG" id="ENOG502SNH0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060680_0_0_1"/>
    <dbReference type="OMA" id="SEEGNWA"/>
    <dbReference type="OrthoDB" id="642536at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165:SF34">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="2">
        <location>
            <begin position="94"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ91139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="369" mass="41023" checksum="E3FAE132A04BCA97" modified="2012-06-13" version="1">MATEKRRRHHSPPVITSGDSSSSIPTTSVHSASPPLDLLPDIARRLTSLEDFFSLRASCTAYRALLPASRDLFASQAPLLLVSLFPSFSEALFHPRLRRVHRFRLPWGHHLPPSRHTLFYAHGFLVTATTAATQYPPRLLLLHLFTGEQQRLPKVPTPFSRVILSDDLLAVLFLPGRPTIQHCHPGDALWRVAAADTPHVFDDLIFVDSTLYALVGLRLAILELSESSLELSFLGGEYDEETRPVGERFMLGECEGEVLLISEEHGETVVYRVFRWVSGEGKWAMITSLGGRTLFLGFNGFAACVGPGLPEIRGDCIYAAGRRLGEWYEYSLVNGTCDVCYAEYVGAPPLNNNSPIRPLVWVFPSLMQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1IT21</accession>
    <name>I1IT21_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100823810</name>
        <name evidence="15" type="ORF">BRADI_4g38575v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer (believed to be a pentamer but probably hexamer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576869.1">
        <property type="nucleotide sequence ID" value="XM_003576821.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IT21"/>
    <dbReference type="EnsemblPlants" id="KQJ91596">
        <property type="protein sequence ID" value="KQJ91596"/>
        <property type="gene ID" value="BRADI_4g38575v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823810"/>
    <dbReference type="Gramene" id="KQJ91596">
        <property type="protein sequence ID" value="KQJ91596"/>
        <property type="gene ID" value="BRADI_4g38575v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823810"/>
    <dbReference type="eggNOG" id="ENOG502QYH0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015790_0_3_1"/>
    <dbReference type="OMA" id="SNTINQG"/>
    <dbReference type="OrthoDB" id="1546383at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000098">
        <property type="entry name" value="Germin-like protein 9-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095476" description="Germin-like protein" evidence="13">
        <location>
            <begin position="18"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="67"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQJ91596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="213" mass="22372" checksum="91765AED255CF8A1" modified="2012-06-13" version="1">MASIKLVFVVLAVLVSAAPMAVVAGDPDILTDFVAPTYIPGIPATNITGDFFTYTGFAFPLPPLMTFSVLKASMAQFPALDGQSVSYAKLRFPPGTVNPTHTHPRASELLFLLEGALSVGFIDTAGKLYAKDLAAGDMFVFPKGLVHYQYNPGNILAVAFSAFGSAFAGTVNVPSVVFGTDIDDAVLAKSFKTDVPTVLKLKAALTPPPTPMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVN8</accession>
    <name>I1IVN8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100843732</name>
        <name evidence="2" type="ORF">BRADI_5g01310v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580224.1">
        <property type="nucleotide sequence ID" value="XM_003580176.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239636.1">
        <property type="nucleotide sequence ID" value="XM_010241334.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239637.1">
        <property type="nucleotide sequence ID" value="XM_010241335.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVN8"/>
    <dbReference type="STRING" id="15368.I1IVN8"/>
    <dbReference type="EnsemblPlants" id="KQJ81534">
        <property type="protein sequence ID" value="KQJ81534"/>
        <property type="gene ID" value="BRADI_5g01310v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ81535">
        <property type="protein sequence ID" value="KQJ81535"/>
        <property type="gene ID" value="BRADI_5g01310v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ81536">
        <property type="protein sequence ID" value="KQJ81536"/>
        <property type="gene ID" value="BRADI_5g01310v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843732"/>
    <dbReference type="Gramene" id="KQJ81534">
        <property type="protein sequence ID" value="KQJ81534"/>
        <property type="gene ID" value="BRADI_5g01310v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81535">
        <property type="protein sequence ID" value="KQJ81535"/>
        <property type="gene ID" value="BRADI_5g01310v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81536">
        <property type="protein sequence ID" value="KQJ81536"/>
        <property type="gene ID" value="BRADI_5g01310v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843732"/>
    <dbReference type="eggNOG" id="ENOG502QW1F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043458_3_1_1"/>
    <dbReference type="OMA" id="KGWKIIK"/>
    <dbReference type="OrthoDB" id="1921166at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012866">
        <property type="entry name" value="DUF1644"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31197:SF2">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31197">
        <property type="entry name" value="OS01G0612600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07800">
        <property type="entry name" value="DUF1644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ81534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="373" mass="42290" checksum="C54C44FA2D14F5A5" modified="2012-06-13" version="1">MPNGENKLFKMPNADGEISALHKEWDDARCPICMDHPHNAVLLLCSSHDKGCRSYICDTSYRHSNCLDRFKKMKLDHMDSSSQPSSSFPRDPSNRNVAQISRVGLNRESPRLLIDIPDRGDLGHQHVIHSSAAIAGQQEETNFIQDTDLTLVAQEGEGSRLVESGEAANLNQLMCPVCRGTVEGWEIIKDARQYLDEKPRACSREACTFSGNYSALRRHARRVHPTTRPADVDPSRRRAWHRLEHQREYGDILSAIRSAMPGAVVLGDYVIEGGDMSSHDREGSGPNQPSGSLLTTFFLFHMMSTTPMRSGDEPRGSSRGLRRQRRRYLWGENLLGLQYDDDDDEEGDDNLAEEVQRPRSHRRFVRSRSEERH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZA3</accession>
    <name>I1IZA3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <ecNumber evidence="12">4.1.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100821874</name>
        <name evidence="18" type="ORF">BRADI_5g14640v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>S-adenosyl-L-methionine + H(+) = S-adenosyl 3-(methylsulfanyl)propylamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="12">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the eukaryotic AdoMetDC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="12"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311365.1">
        <property type="nucleotide sequence ID" value="XM_024455597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZA3"/>
    <dbReference type="STRING" id="15368.I1IZA3"/>
    <dbReference type="EnsemblPlants" id="KQJ83381">
        <property type="protein sequence ID" value="KQJ83381"/>
        <property type="gene ID" value="BRADI_5g14640v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821874"/>
    <dbReference type="Gramene" id="KQJ83381">
        <property type="protein sequence ID" value="KQJ83381"/>
        <property type="gene ID" value="BRADI_5g14640v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0788">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023050_2_0_1"/>
    <dbReference type="OMA" id="RNWHIYS"/>
    <dbReference type="OrthoDB" id="1068353at2759"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006597">
        <property type="term" value="P:spermine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.50:FF:000001">
        <property type="entry name" value="S-adenosylmethionine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.90.10:FF:000002">
        <property type="entry name" value="S-adenosylmethionine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.50">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.90.10">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048283">
        <property type="entry name" value="AdoMetDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001985">
        <property type="entry name" value="S-AdoMet_decarboxylase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018166">
        <property type="entry name" value="S-AdoMet_deCO2ase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00535">
        <property type="entry name" value="SAM_DCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570:SF24">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01536">
        <property type="entry name" value="SAM_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001355">
        <property type="entry name" value="S-AdenosylMet_decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01336">
        <property type="entry name" value="ADOMETDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4 12" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 12" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9 12" id="KW-0704">Schiff base</keyword>
    <keyword evidence="5 12" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5013990406" description="S-adenosylmethionine decarboxylase beta chain" evidence="17">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013990405" description="S-adenosylmethionine decarboxylase alpha chain" evidence="17">
        <location>
            <begin position="77"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="13">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="13">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="13">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="13">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="16">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="15">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR001355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQJ83381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="396" mass="43288" checksum="827A73B95FAF63BE" modified="2012-06-13" version="1">MAVLSVDPAPVSAIGFEGYEKRLEITFSEASVFADPHGRGLRALSRAQIDSVLDLARCTIVSELSNKDFDSYVLSESSLFIYSQKIVIKTCGTTMLLLTIPRILELAEELCMPLAAVKYSRGMFIFPGAQPAPHRSFSEEVDVLNRYFGGLKSGGNAYVIGDPAKPGQKWHIYYATEQPEKPMVTLEMCMTGLDKKKASVFFKTSADGHISCAKEMTKVSGISEIIPEMEVCDFDFEPCGYSMNAIHGSAFSTIHVTPEDGFSYASYEVMGIDASALAYGDLVKRVLRCFGPSEFSVAVTIFGGRGHAATWGKKLTAEAYDCNNTVEQELPCGGVLIYQSFAVNEEVAVSAGSPRSVFHCFDGENVENHALVKDGKLANLLQWREDALEEDGVLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1J2I2</accession>
    <name>I1J2I2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100826721</name>
        <name evidence="3" type="ORF">BRADI_5g23790v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580670.1">
        <property type="nucleotide sequence ID" value="XM_003580622.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J2I2"/>
    <dbReference type="FunCoup" id="I1J2I2">
        <property type="interactions" value="666"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J2I2"/>
    <dbReference type="EnsemblPlants" id="KQJ84926">
        <property type="protein sequence ID" value="KQJ84926"/>
        <property type="gene ID" value="BRADI_5g23790v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826721"/>
    <dbReference type="Gramene" id="KQJ84926">
        <property type="protein sequence ID" value="KQJ84926"/>
        <property type="gene ID" value="BRADI_5g23790v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826721"/>
    <dbReference type="eggNOG" id="KOG1454">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_35_1_1"/>
    <dbReference type="OMA" id="LHDWGGM"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000168">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050228">
        <property type="entry name" value="Carboxylesterase_BioH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194">
        <property type="entry name" value="HYDROLASE ALPHA/BETA FOLD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN LPX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="139"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ84926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="392" mass="42797" checksum="712731881969CCAD" modified="2012-06-13" version="1">MAGCPCASTSSASSSPLFLLPSVSTPSRSSVAFASCGVRLRKAGARRGVAASSSNKEDTEEQGDEGGDPAFNPFGFVTDNPSSRAAIQLQPSPAEDGNVGQMLYRTEYKGREYGKTVRSGDLRWFVRETGSPDARRGTIIFIHGAPTQSFSYRMVMSQMSDAGYHCFAPDWIGFGFSEMPQPGYGFDYKEEEFHKAFDDLLGTLNVTEPFFLVVQGFLVGSYGLTWALKNSSKVRKLAILNSPLTVSSPVPGLFNQLRLPLFGEFTCQNAILAERFIEAGSPYVLKSEKADVYRLPYLSSGAPGFALLEAARKAKFQDVLSRISAGFSSNSWEKPILLAWGESDKYLPLSIAEEFKKSNPAVVKLKTIEGAGHMPQEDWPEKVVTALTSFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E2W9</accession>
    <name>A0A0Q3E2W9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box protein AT5G49610-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g04650v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E2W9"/>
    <dbReference type="FunCoup" id="A0A0Q3E2W9">
        <property type="interactions" value="813"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ81982">
        <property type="protein sequence ID" value="KQJ81982"/>
        <property type="gene ID" value="BRADI_5g04650v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81982">
        <property type="protein sequence ID" value="KQJ81982"/>
        <property type="gene ID" value="BRADI_5g04650v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E2W9"/>
    <dbReference type="OrthoDB" id="592687at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3E2W9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056594">
        <property type="entry name" value="AT5G49610-like_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33207">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23635">
        <property type="entry name" value="Beta-prop_AT5G49610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box protein AT5G49610-like beta-propeller" evidence="1">
        <location>
            <begin position="120"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ81982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="415" mass="47720" checksum="7DF76E6180EDFA4E" modified="2016-01-20" version="1">MDSPPSPAAVANIMSKVLDDDNLLREIILRVGFPTTLFRATLVCKRWFHHAADPAFLRRFRELHPPRLLGLYVKDVFFPYRPNVISRFVPLLTQTEEPDLATILRRMTTISLDTDAEIRIRECWNGSVFISEFRKYGPIIYQVHNPLFPERGVAVLPPPPQHAMTKHINGYIFTMSCFLSKGGDGCLSYEWLAMEIHFMGRRSIGEIFGKVYVLQDGAWVMRTEATTQLPEPQWKPQPLLVNDRIYMAGGTCNNILVLDLANSSFFTIQLPEGVELSIRYKEGIDTAFSRADASGVYLIGVKELQLRIWLYEDDNFFLVDTICLREMRAALGMPDVMVEEEHTDAVRISQAGDNAEFVFLQMGRCRFYLDIRRRVMRKVYEVTEKDQRAGQVHPFMMIWPPAFPSLKDDSTRNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EFX6</accession>
    <name>A0A0Q3EFX6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NPH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100835792</name>
        <name evidence="5" type="ORF">BRADI_5g25950v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014750987.1">
        <property type="nucleotide sequence ID" value="XM_014895501.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EFX6"/>
    <dbReference type="STRING" id="15368.A0A0Q3EFX6"/>
    <dbReference type="EnsemblPlants" id="KQJ85260">
        <property type="protein sequence ID" value="KQJ85260"/>
        <property type="gene ID" value="BRADI_5g25950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835792"/>
    <dbReference type="Gramene" id="KQJ85260">
        <property type="protein sequence ID" value="KQJ85260"/>
        <property type="gene ID" value="BRADI_5g25950v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835792"/>
    <dbReference type="OrthoDB" id="1080584at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EFX6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="NPH3" evidence="4">
        <location>
            <begin position="231"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="178"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="503"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="554"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="567"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ85260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="577" mass="62127" checksum="7F522AEE43D7B57E" modified="2016-01-20" version="1">MDEACDLEVHVNGHHTLLLHQSVVCGFSGKLRAMVATTKKKKPGKEAEPAAGALLSVELAGFPGGGEALELAARFCYGNGEPPPLLWRPTTFPLLYCAAVFLEMTEEVRPGNLLSQAEAFVHDDARRLWTWADAVAAVRSCERLVVSDSSTNAAAIADALLEKLVSALFSRIAAASRPANCSSSSSSSSPLSISSSSSSSPDTDSATKTPPPPELVTTTTKPCSSSGSGNEWWFDNVASLSPPTVEKATRFLGGEKNNKDPTITRFLLHYLRRRRRSVDSPTSDDLTLSVLANTAAHGVAALAATGGCFSSCRSLFRVLRTVSAAGASRESRNKLETLVGSLLDQATLDDLLVPVFVSGGGSVPGGGVYDVRLVVRLVRVFVMAMALEEEEEGTKRLRKVGRLVDKYLGEISPDQGLTVGRFLAVAESLPDSARGCFDGVYRALDIFLQSHAALTVEERAAACRCVNYEKLTLEACRDMARNRRVPAAVAVQALASQRWRPDKLRNVHPPSPTPTPIPVGDEEEAPRLSLWPIRRQSRGSGELETAAYFKEMTGDRRRTSKLQGRKSFGGRGLPWMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EPE5</accession>
    <name>A0A0Q3EPE5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_4g16065v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EPE5"/>
    <dbReference type="EnsemblPlants" id="KQJ88156">
        <property type="protein sequence ID" value="KQJ88156"/>
        <property type="gene ID" value="BRADI_4g16065v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88156">
        <property type="protein sequence ID" value="KQJ88156"/>
        <property type="gene ID" value="BRADI_4g16065v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EPE5"/>
    <dbReference type="OrthoDB" id="3990054at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EPE5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23995">
        <property type="entry name" value="Seipin_BSCL2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009617">
        <property type="entry name" value="Seipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212">
        <property type="entry name" value="BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212:SF5">
        <property type="entry name" value="SEIPIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06775">
        <property type="entry name" value="Seipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ88156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="210" mass="22095" checksum="6D9BAF03E005065D" modified="2016-01-20" version="1" fragment="single">MCLTSFDTISYRWGPPGPHVRFSGLLAYLCSRALSAFLGLLLLPTASLVGALRRAAPALARGALAFAALSAASLLLGFFLVRRCSVEGPISVRRPLYFDYTKQQPSVAVSLAGAAASIPAGHSVKVSAALLLPDSDHNYRIGVFQIKAEAIAATGVPIATATRPYMLRYKSDAVRLAQSALAIVPLTTGLMSESQSATIRDVPSDVRAPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FAA9</accession>
    <name>A0A0Q3FAA9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100827290</name>
        <name evidence="5" type="ORF">BRADI_3g26900v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FAA9"/>
    <dbReference type="STRING" id="15368.A0A0Q3FAA9"/>
    <dbReference type="EnsemblPlants" id="KQJ96743">
        <property type="protein sequence ID" value="KQJ96743"/>
        <property type="gene ID" value="BRADI_3g26900v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96743">
        <property type="protein sequence ID" value="KQJ96743"/>
        <property type="gene ID" value="BRADI_3g26900v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="37537at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FAA9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19145">
        <property type="entry name" value="AKR_AKR13D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF79">
        <property type="entry name" value="PROTEIN PCNT115, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="20"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ96743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="358" mass="39043" checksum="639194893B4EA4CF" modified="2016-01-20" version="1">MEQSQMARVKLGTQGLEVSKIGYGCMGLTGVYNAPVPEDAGVAVIRRAFDAGVTFFDTADAYGPHTNEILLGKALKQVPRQSVQVATKCGIAGFDISGMVVKGTPDYVRACCEASLARLGVDYIDLYFQHRIDQSVPIEETMGELKKLVEEGKVKYVGLSEASADTIRRAHAVHPITAVQLEWSLWTRDIEEDIIPLCRELGIGIVPYSPLGRGFFAGRAAVESIPSESLLSKHPRYTGENLEKNKALYTRLEKLSTKYGCTPAQLALAWVLHQGDDVVPIPGTTKVKNLDDNIGAVKAIGNDSLQWSGRAAHGVPFRRKTSLGAAGQSRVDLEALQLPIMHFEHHRGTRRGRPPGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FHY6</accession>
    <name>A0A0Q3FHY6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_3g42037v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FHY6"/>
    <dbReference type="EnsemblPlants" id="KQJ99254">
        <property type="protein sequence ID" value="KQJ99254"/>
        <property type="gene ID" value="BRADI_3g42037v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99255">
        <property type="protein sequence ID" value="KQJ99255"/>
        <property type="gene ID" value="BRADI_3g42037v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68537">
        <property type="protein sequence ID" value="PNT68537"/>
        <property type="gene ID" value="BRADI_3g42037v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99254">
        <property type="protein sequence ID" value="KQJ99254"/>
        <property type="gene ID" value="BRADI_3g42037v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99255">
        <property type="protein sequence ID" value="KQJ99255"/>
        <property type="gene ID" value="BRADI_3g42037v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68537">
        <property type="protein sequence ID" value="PNT68537"/>
        <property type="gene ID" value="BRADI_3g42037v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FHY6"/>
    <dbReference type="OrthoDB" id="6161812at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FHY6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF999">
        <property type="entry name" value="NB-ARC DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="3">
        <location>
            <begin position="4"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="4">
        <location>
            <begin position="247"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="366"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ99255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="749" mass="85376" checksum="73FB6DB0784C792C" modified="2016-01-20" version="1">MEKAGQLKVVAVVGSGGMGKTTLAKQVYTKIKGQFDCTAFVPVSQNPNIVKILSDIILQVHACLPEGCNDERPLIHKLREYLQYKRYLIVIDDLWATEAWSIKNSFVENNRGSRVITTTHIERVAQACCSRFHDHVYKIQPLNVPDSRRLFHRRIFLSNDACPEHLKDVSDEILKKCQGVPLAILSVASTLSSHGGVMFKKNWETMRDHLGDPLETDSDMNWMRYVLNLGYNDLSLDLKTCLLYIGIFPEDSVIMKDDLIRRWIAGCFVSGKNTYGPEEIADSYFNELINRNMIQIADYDDCGEVASCRVHDLMLDFIISKSTEENFMTVINGQRSTKGTLEPRQVSVHLSNSEPNNRLENMPLTQVRSFYFWGPAQLMPSFSKFQLLRVLYLDVSGAKREHCYLSSIGNLFQLRYLRTRGIPYKQVLTQLQNLQRLKTLEIAEDDGIDGQYDYFVLNVCLLPSTLLHLIVTADVKLVGGIGRMRSLHTLATLQVNLRDVESMKGLGDLSNLRDLKLLRCTYGVEDTCDLLVSSLCRLSSLKSLAFRGSLMADALTRWSPPPLDLQRLHVLECPFSTVSDWLTQLDRLRSLEVTVQLLPRDGAEILARLTSLVHLRLHTRAQAPEEGVIIRGAMFPYLKNFWFRCKVPCLMFEAGAMPRIQSLTVECHVHAERHAYDLHHGIEHLGSLKVYKMFAYERDTFMSRFLHSSYSSKAHVEGVRKWDLQTLETEVRKVIDKHPGGPDVSIKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FSL0</accession>
    <name>A0A0Q3FSL0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100832472</name>
        <name evidence="8" type="ORF">BRADI_3g59992v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK02039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573130.3">
        <property type="nucleotide sequence ID" value="XM_003573082.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FSL0"/>
    <dbReference type="STRING" id="15368.A0A0Q3FSL0"/>
    <dbReference type="EnsemblPlants" id="KQK02039">
        <property type="protein sequence ID" value="KQK02039"/>
        <property type="gene ID" value="BRADI_3g59992v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832472"/>
    <dbReference type="Gramene" id="KQK02039">
        <property type="protein sequence ID" value="KQK02039"/>
        <property type="gene ID" value="BRADI_3g59992v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832472"/>
    <dbReference type="OrthoDB" id="608258at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FSL0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07331">
        <property type="entry name" value="M48C_Oma1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051156">
        <property type="entry name" value="Mito/Outer_Membr_Metalloprot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726:SF26">
        <property type="entry name" value="PEPTIDASE M48 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M48" evidence="7">
        <location>
            <begin position="216"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK02039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="396" mass="44399" checksum="5607F9EE220D9F2B" modified="2016-01-20" version="1">MKCILKRTAGSALRLLPPPPPPLQAATRPFHRGGSQAPPAELSFLRRCSSFHTSARRRPEPELIHFARRRRGEGEGEGGGRIPWYLSWEKLLTRALAPAAAACALYRLSLQTVPYTHRRRAVVLPARYERKLGERRFQALKEKAAAAGKLLPPDHGDAVRARRVAEEIVAAARRTLIGRRGNEDLLLLLDDDAESRDEPRGAPEPEPMTKHLVGLDWEVIVVEDDEASASCLPGGKIVVNTGFLRRFQTDAEIAVVLGREVGHIVARHAAEGFSKALWSELLSICLWGVFDCRDFVARTLPLLLVKRHFSRKMEIEADHIGIMLLAAAGFDPHVALEVHKKLRDLGGESELRNYLSTHPSRRKRVQNLSQHKLMEEAMELYRETNARKTANVYSCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GBT1</accession>
    <name>A0A0Q3GBT1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-adaptin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100825405</name>
        <name evidence="9" type="ORF">BRADI_2g44070v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Adaptor protein complexes are heterotetramers composed of two large adaptins (beta-type subunit and alpha-type or delta-type or epsilon-type or gamma-type subunit), a medium adaptin (mu-type subunit) and a small adaptin (sigma-type subunit).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232046.2">
        <property type="nucleotide sequence ID" value="XM_010233744.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GBT1"/>
    <dbReference type="STRING" id="15368.A0A0Q3GBT1"/>
    <dbReference type="EnsemblPlants" id="KQK08816">
        <property type="protein sequence ID" value="KQK08816"/>
        <property type="gene ID" value="BRADI_2g44070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825405"/>
    <dbReference type="Gramene" id="KQK08816">
        <property type="protein sequence ID" value="KQK08816"/>
        <property type="gene ID" value="BRADI_2g44070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825405"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GBT1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000014">
        <property type="entry name" value="Beta-adaptin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000113">
        <property type="entry name" value="Beta-adaptin-like protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015151">
        <property type="entry name" value="B-adaptin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09066">
        <property type="entry name" value="B2-adapt-app_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01020">
        <property type="entry name" value="B2-adapt-app_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Beta-adaptin appendage C-terminal subdomain" evidence="8">
        <location>
            <begin position="725"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="644"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="655"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="674"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK08816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="847" mass="93244" checksum="4F5DFD2641B8A73C" modified="2016-01-20" version="1">MAPTAPSPAKSASPSQPSGKSEVSDLKQQLRQLAGSRAPDADDQRRDVFKRVISCMTAGIDVSAAFGEMVLCSATSDVVLKKMCYLYVGVHARAHPDLALLTINFLQRDCRDQDPTIRGLALRSLCSLRVPNLVEYLVAPLTTGLKDPSAYVRMVASVGAAKLYHISTTTCLDADLPAALKALMLSDSDAQVVANCLHSLLEIWTLEAANSEAAAREIETLYSKPVVFYLLNRIKEFSEWAQCHVLELASKFLPSDNNEIFDIMNLLEDRLQHANGAVVLATIKVFLHLTMSMTDVHQQVYERIKAPLLTLVGAGSPEQSYSVLCHLHLLVMRAPMLFSSDYKSFYCQFSDPSYVKKLKLEMLTAIANESNTYEIVTELCEYAGNVDVPIARESIRAVGKIALQQYDVNAIVDRLLQFLEMDKDYVTAETLVLVKDLLRKYPQWSHDCIAVVGNISSQNIQEPKGKAALIWMLGEYSQDMHDAPYILESLVDNWDEEQSPEVRLHLLTAVMKCFFKRPPETQKALGATLAAGLADTHQDVHDRALFYYRLLQHDPAVAERVVNPPKQAVSVFADTQSSEIKDRIFDEFNSLSVVYQKPSYMFTDKEHRGTFEYSEDLTNLTVGVEAPETVISAQRYQENDNDLLLSTSDKEDNVTRASNGSSTSTYNAPPDLTGPSSLLSSQTPTETALINPGGPTYSAQTNFSLDDLLGLGVPDTPAPPSPPALTLNSKPVLDPGTFQKKWGQLALSLSQECSLSPQGAASLMNPQSLIRHMQSNYIQCIASGGQPPNYKFFFYGQKDGATAFYLVECIVNTASAKAQLKIKADDGTTAQSFSTLFQSALSKFGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GNG7</accession>
    <name>A0A0Q3GNG7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g08523v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GNG7"/>
    <dbReference type="EnsemblPlants" id="KQJ82346">
        <property type="protein sequence ID" value="KQJ82346"/>
        <property type="gene ID" value="BRADI_5g08523v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82346">
        <property type="protein sequence ID" value="KQJ82346"/>
        <property type="gene ID" value="BRADI_5g08523v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GNG7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ82346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="195" mass="21124" checksum="33E8BD43FF725ECF" modified="2016-01-20" version="1">MASDKKSVPQGISTRGNYSPLLSRTPPLFSLPPDLSTSSLSLLRAPRPDSKAPPMAATDCHGATPPPHVSTRLHPARRRGIRPPSGRRHRIHPPWIPGGRLSPSPTPTAARYARSGDLDPGCCRIWRPRPRAPPNPPLPLTSSSAPMRTSRPGGSSCRAGVPDLSHRRGEPEAQPSPSWSRNRSHRRHGAVADFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GSS6</accession>
    <name>A0A0Q3GSS6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_1g13261v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GSS6"/>
    <dbReference type="EnsemblPlants" id="KQK13903">
        <property type="protein sequence ID" value="KQK13903"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74351">
        <property type="protein sequence ID" value="PNT74351"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74352">
        <property type="protein sequence ID" value="PNT74352"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74353">
        <property type="protein sequence ID" value="PNT74353"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74354">
        <property type="protein sequence ID" value="PNT74354"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74355">
        <property type="protein sequence ID" value="PNT74355"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74356">
        <property type="protein sequence ID" value="PNT74356"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74357">
        <property type="protein sequence ID" value="PNT74357"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74358">
        <property type="protein sequence ID" value="PNT74358"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74359">
        <property type="protein sequence ID" value="PNT74359"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74360">
        <property type="protein sequence ID" value="PNT74360"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13903">
        <property type="protein sequence ID" value="KQK13903"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74351">
        <property type="protein sequence ID" value="PNT74351"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74352">
        <property type="protein sequence ID" value="PNT74352"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74353">
        <property type="protein sequence ID" value="PNT74353"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74354">
        <property type="protein sequence ID" value="PNT74354"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74355">
        <property type="protein sequence ID" value="PNT74355"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74356">
        <property type="protein sequence ID" value="PNT74356"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74357">
        <property type="protein sequence ID" value="PNT74357"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74358">
        <property type="protein sequence ID" value="PNT74358"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74359">
        <property type="protein sequence ID" value="PNT74359"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74360">
        <property type="protein sequence ID" value="PNT74360"/>
        <property type="gene ID" value="BRADI_1g13261v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GSS6"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43670">
        <property type="entry name" value="HEAT SHOCK PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43670:SF64">
        <property type="entry name" value="SHSP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="SHSP" evidence="8">
        <location>
            <begin position="158"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="91"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="248"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="350"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="277"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="355"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK13903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="454" mass="50043" checksum="E6B32DE70E140466" modified="2016-01-20" version="1">MAWPHTESKFKMVDRATGAMHGPTLNREKENLLYLLYPANMLLLSHGLTSPANPPATCQPPKTKQGILDRSLCVADARMEEEHQKMEKNITCPASHVRPVQESYSSPSPEPAPLPVSDYIYDLHSKTPPSSHSSSSSSTSISLHTFIELKAMDTRGISRTFEDYAPIVEWSHAADASTVKIIVPGFKREEIRVLVDNHGHLRTRGERPLEGSKWSRFQKDFQLPSDCNVDGIRAKFENEALTITLPKKNPSPAAIPMPMPIPAKPEPVRPAPASAAPLPPRRPPPALPEPAFPERRPSLPRKPSAQEPAWPAPAPAPEVMESQIPNKYNGGAAAAATAELQKKQQAEAVAAEEEEMKRREREARGKMEEDNRKMMEERKLANEEEEERRRMMGEMEMAQQRRRPATATAASRGLLVNVAVAVVVLVGITVYVWHNLTSAAGGGGHGNGSYGDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GY42</accession>
    <name>A0A0Q3GY42_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100834057</name>
        <name evidence="7" type="ORF">BRADI_1g22717v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin-like family. Class-II subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752556.1">
        <property type="nucleotide sequence ID" value="XM_014897070.2"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GY42"/>
    <dbReference type="EnsemblPlants" id="KQK15429">
        <property type="protein sequence ID" value="KQK15429"/>
        <property type="gene ID" value="BRADI_1g22717v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834057"/>
    <dbReference type="Gramene" id="KQK15429">
        <property type="protein sequence ID" value="KQK15429"/>
        <property type="gene ID" value="BRADI_1g22717v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834057"/>
    <dbReference type="OrthoDB" id="1668162at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GY42">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051144">
        <property type="entry name" value="Formin_homology_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733">
        <property type="entry name" value="FORMIN-J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733:SF10">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 tensin-type" evidence="5">
        <location>
            <begin position="200"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="6">
        <location>
            <begin position="1170"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="541"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="682"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="750"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="838"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1555"/>
            <end position="1608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="682"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="756"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="840"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="853"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="875"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="915"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="932"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="966"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1555"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1575"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1593"/>
            <end position="1608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK15429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1608" mass="174779" checksum="3206609B59D167C2" modified="2016-01-20" version="1">MALFRKFFLKKTPDRLLEISERVYVFDCCFSADAMGEDEYRDYLSGIVAQLQDYFPDASFMVSNFWSGDKRSRISDILSEYDMTVMDYPQQYEGCPLLQLEMIHHFLKSCENWLSVEGQQNMLLMHCERGGWPVLAFMLAGLLLYRKTYTGEQKTLEMVYKQARRDFIQQFFRLNPQPSHLRYLHYVTRHGSGSEWPPISRPLILDSVVLHTVPRFDSEGGCRPYLRIHGQDSSPGNKSAKVLFEIPKTKKHLQRYGQVEVPVKISAFCRVQGDVVLECIHIGRNIDHEETMFRVMFNTAFIQSNVLGLNRDDIDTAWNVNNQFPRDFRAEVLFSDPDSFKPAAVLEEVGDDGDETDVASVDTGDEFYEAEEDWHDARRDPETQSTDGRTSLEVGNTELDGGVASEANISLEKHLTDEDVKLIVSQNSGSMNDNGPSATAPILENLGGLQQACKVLEKSKLGNRSDQEDNAVHDIQVVACSVDSEGRKFGSICQEDTKGVIAQTLVTATDPSCSDEVQCHPDGCTKISRYTDLDYTAFGAPRTSSGMDEDTHLRTRPNEGQPNGDIKIITENTITVDNELLIYEEKTVVDNGNLMQEVKKVVKEESVISKMDRTRLENMHSRDNSNHKVEMAKIAETADTRLEESKLKSGHGDPILAKKTSLQDSIVVLPANEIAAEIKTKREERGGRRDFGLALPQSKIEARANSPRISDVQRQTPEAASSVPKKIPVDGTAPEPVLLEAIVGHIEQLEQQSKPAKAKTIRRWVSPKKESETASVRRPSHPPSRYDSSPAALAIRSMSTDNKINVVKGASLVLPGVPYGKHISQDAALSSTFPPRLSVTGAQAASRTQATNPAPPPPPPPPPFYSSSSSGTKHLPPPPPPPPPPPPPPPASAGLSNIPPPPPPPFLGLGVRGNVPPPPPPPPRLNGSAIAPPAPPPPPPKPSSSAPPPPPPQPPPAPSFIRHPSAPLPPPPPPPPPTARSVAPPPPPPPLPATRSGAPPPPPPPRTCSTPPPPPPPPMTRSGAPPPPPPPPGGRPGPPPSPPPPGARPGPPPPPPPPGARPGPPPPPPPPGARPGPPAPPPPPGARPGPPPPPGRPGAPPPPPPPGGGGRAPPPPPAPGGRVGGPPPPPPPGGRAPAPPRAPGVPPPPGGNPSSLGRERGVVRSPASGFGAAAARKSTLKPLHWVKVTRAMQGSLWEELQRNDDSQSVSEFDLSELESLFPAAVPKLDNTSKTDRRKSLGSKPEKVHLIELRRANNTEIMLTKVKMPLPELVRAALALDQSTLDVDQVENLIKFCPTKEEMETLKNYTGDKENLGKCELFFLELMKVPRMESKLRVFSFKIQFGSQVADLGKSLKTIDSSCNEIRTSLKLKEIMKKILLLGNTLNQGTARGAAVGFRLDSLLKLTDTRATNNKMTLMHYLCKVLAAKSPQLLNFYVDLVSLEATSKIQLKMLAEEMQAVSKGLEKVEHEFKASESDGPVSEVFREKLKEFTDNAGADVQSLSSLFSEVGKKADQLIKYFGEDPVRCPFEQVMSTLLTFVTMFRKAHEENVKQAEIEKKKAQKEAEAEKTKNAQLASKNESKPSNPSRQAKQMLEKTKATSRRGKDVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H5T4</accession>
    <name>A0A0Q3H5T4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g15677v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H5T4"/>
    <dbReference type="EnsemblPlants" id="KQJ83595">
        <property type="protein sequence ID" value="KQJ83595"/>
        <property type="gene ID" value="BRADI_5g15677v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83596">
        <property type="protein sequence ID" value="KQJ83596"/>
        <property type="gene ID" value="BRADI_5g15677v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83595">
        <property type="protein sequence ID" value="KQJ83595"/>
        <property type="gene ID" value="BRADI_5g15677v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83596">
        <property type="protein sequence ID" value="KQJ83596"/>
        <property type="gene ID" value="BRADI_5g15677v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H5T4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ83595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="151" mass="16576" checksum="0C4282263FA08A34" modified="2016-01-20" version="1">MAIYGKFTFSFHLFYRESCQKAPDHLTHLFIPVPLPRPKSQPNPLSVFFASFILPVLLFNQQTILDLAWRGDRRSGRWGDPPGGELLAVVRWWSASNGGGGGGLTGGGRDRCPALGSLVSPRRRGEVPAEVSKLYGDAAHPLGHVLHHLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HGG3</accession>
    <name>A0A0Q3HGG3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g61897v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HGG3"/>
    <dbReference type="FunCoup" id="A0A0Q3HGG3">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK21605">
        <property type="protein sequence ID" value="KQK21605"/>
        <property type="gene ID" value="BRADI_1g61897v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21605">
        <property type="protein sequence ID" value="KQK21605"/>
        <property type="gene ID" value="BRADI_1g61897v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HGG3"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000047">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF223">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK21605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="496" mass="53628" checksum="799AC57176A517EF" modified="2016-01-20" version="1" fragment="single">MSNHAAASDEANNKADAAHFLFVPLMAQGHIIPAVDTALLLATQGALCTIVATPSTAARVRPTVDSARLSGLAVTLVDFPLDYAAVGLPGGMPGGADNMDNIPLEHMLSYYRAIALLREPIESYLRAAHAPRPPTCVVSDFCHPWTRELAASLGVPRLSFFSMCAFCILCQHNVERFNAYDGVLDPNEPVVVPGLEKRFEVTRAQAPGFFRGWPGWEQFGDDVETARAQADGVVINTFLEMEPEYVAGYTAARGMKVWTVGPVSLYHQHTATLALRGDTTAIDADECLRWLDGKEPGSVVYASFGSIVHADPKQVSELGLGLEASGHPFIWVVKDAARHDETALAFLRGLEARVAGRGLLVWGWAPQALILSHRAAGAFVTHCGWNSTLEAVTAGLPVVTWPHFTDQFLNEKLAVEVLEIGVSVGVKEPVLYQVDQKEIVVGRETVEAAVRSVMDGGEEGEERRRRARALAGKAKAAMREGGSSHSNIRDLVKCFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HIN9</accession>
    <name>A0A0Q3HIN9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g69005v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
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</entry>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
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                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
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            <strain evidence="8">cv. Bd21</strain>
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        <property type="protein sequence ID" value="KQJ87013"/>
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        <property type="gene ID" value="BRADI_4g08988v3"/>
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    <dbReference type="OrthoDB" id="680064at2759"/>
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        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ87013"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="646" mass="74892" checksum="1E2E774DCB6F7930" modified="2018-03-28" version="2" fragment="single">ASSCTPECDENLKPAVGMAFDNMEEVEEFYKAYAHNVGFSVRIGQKRTVDNVVVWRRFLCGKSGFRRNNEEEPKKELNGEKKGDEKKRTHARKITRCGCEAMITVKRMKDGKYAVSYFHEEHTHEFVTPRKQHLIKSNRQVSDKAKDTLFTCHAASIGTAGAFRLLRVGEGGFEFVGCTKRDLQNYHRDMRSTFKDSDAQMFIDNLRKRQEINPGFFFEYVLDDKNWLTHVFWADACCRKNYALFGEMVSFDSTYSTNQYSMIFCPFTGINHHMASVFYGAALIVNETIESYKWVFQTFLKAMDGAAPRLIVTDEDQSMKIAIEDVMHNTVHRLCMWHIMRKLPEKVGPPLREDEHFYSTINSCVWGSENPTEFEAKWSSMISEFGLEDNTWFSRRYELHESWIPAYFRGVFLGEILRTTSRSESANSFFNHFIGYKHALVEFWIRIGTALEEQRQNELKAGHECLNSMPPLITSWEIEKHASLFFTHAFFSSFQKEVRASRDHCLIENISQEGDVRTTIIGGARSFKSREVQFNTTSKSARCSCMLFETRGIPCRHIIMVLRGAKMSELPSHFILDRWKKTVNRRVVYDSSGNLLEENESSSLDLAKRKMVSEIHQTLDDTIRLAEQSPEELEALRNLLVDMKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LDV7</accession>
    <name>A0A0Q3LDV7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_4g33755v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LDV7"/>
    <dbReference type="FunCoup" id="A0A0Q3LDV7">
        <property type="interactions" value="313"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ90757">
        <property type="protein sequence ID" value="KQJ90757"/>
        <property type="gene ID" value="BRADI_4g33755v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ90757">
        <property type="protein sequence ID" value="KQJ90757"/>
        <property type="gene ID" value="BRADI_4g33755v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LDV7"/>
    <dbReference type="OrthoDB" id="669288at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36479:SF11">
        <property type="entry name" value="NPH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36479">
        <property type="entry name" value="ULP_PROTEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="438"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="295"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="323"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ90757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="565" mass="63123" checksum="A7485DE84E0CA722" modified="2016-01-20" version="1">MVRKRGEVDSGDEDLKPVGVRNRASPYGLTKLYKGFSGAQKLDFRNMEFTSFLHINTTKLHNKSIDWLASCYDPAARGLLIPGRGRIPMTEESVYNALGCPHGTEPVPYKVDKDIEARLAPEMFPGMNLSKTPLHSQVNTILKNMTESGDRFKRLVLLYIMSTILTPTTTTRISNRCYPVLDDIANVHNYNFCKFVLDQLDENLSKKKLNKGCRCSPFLHVAWAATILTAWTNSLIDEVMKADTKEDGSFGKLQMRNKVETIVGQFASGMTCLVGNLVQGWTGLTPPESEEMTRRFATVTGGVPRRSRTARGRFEGFTYPSDTDTDDEEQELDGCDSSDGDPPHNQDGDNQDVGMDGDDDHEGQGSGADGGNNNDRGAHTDNDEAPCNDGPGNEHCGGGVVPASGSRAVHAVSPAVGSASRPVMFPFLQLWGESEKEKEGHWYSISLNINQRKFEILDSLRGPKDDELILHSGEMVLHIKRAWREHYAGAKIQIQDFTTEHIVVPMQDNIDDRGYYMIEFMKKWDGRIVPALVPDDIFEARKILTHQLIMNQDFNEKRNAKEFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LWA5</accession>
    <name>A0A0Q3LWA5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g25404v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LWA5"/>
    <dbReference type="EnsemblPlants" id="KQJ96589">
        <property type="protein sequence ID" value="KQJ96589"/>
        <property type="gene ID" value="BRADI_3g25404v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96589">
        <property type="protein sequence ID" value="KQJ96589"/>
        <property type="gene ID" value="BRADI_3g25404v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LWA5"/>
    <dbReference type="OrthoDB" id="695490at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055302">
        <property type="entry name" value="F-box_dom-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141:SF47">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ96589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="88" mass="9929" checksum="DCBDFBC98C92DA82" modified="2016-01-20" version="1">MPRWWPPGADLISSLPDGVLGEIIYLLPIKEGACTRILVHRWRDLWFVVPLKLDCCQLCGTVSKIRDLTPHIVKAHPGPVPRFYVAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LZF6</accession>
    <name>A0A0Q3LZF6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutathione transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100837891</name>
        <name evidence="7" type="ORF">BRADI_3g32370v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Phi family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572027.1">
        <property type="nucleotide sequence ID" value="XM_003571979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LZF6"/>
    <dbReference type="STRING" id="15368.A0A0Q3LZF6"/>
    <dbReference type="EnsemblPlants" id="KQJ97637">
        <property type="protein sequence ID" value="KQJ97637"/>
        <property type="gene ID" value="BRADI_3g32370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837891"/>
    <dbReference type="Gramene" id="KQJ97637">
        <property type="protein sequence ID" value="KQJ97637"/>
        <property type="gene ID" value="BRADI_3g32370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837891"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000004">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF28">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="104"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ97637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="255" mass="28293" checksum="C92B6E27E43B4469" modified="2016-01-20" version="1">MAPAPVLQVFGQPTSTDVARVMACLFERDLHFELVRTDAITKRGLNLAASCFVDTRYCCPGGGHVTLKHGDTYLTDSRDICRYVCTELPRGRAAREAQALYGAGSLERASIEKWLRAEARCFNPPSSELASNYQQQHLALTPDDEAVAEGERRVRAVMGVYDGALARTRYLAVDEFSLADLCHLPGAHRLLAVAGGERGKRRLLGSLEHVSRWYHDVSARPAWRRVVAVQSAARDLMPPLVPARRCHEELRTPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3N7U3</accession>
    <name>A0A0Q3N7U3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_1g05707v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3N7U3"/>
    <dbReference type="STRING" id="15368.A0A0Q3N7U3"/>
    <dbReference type="EnsemblPlants" id="KQK12734">
        <property type="protein sequence ID" value="KQK12734"/>
        <property type="gene ID" value="BRADI_1g05707v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12734">
        <property type="protein sequence ID" value="KQK12734"/>
        <property type="gene ID" value="BRADI_1g05707v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3N7U3"/>
    <dbReference type="OrthoDB" id="1890356at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829:SF2">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01435">
        <property type="entry name" value="NPOXDRDTASE5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal" evidence="11">
        <location>
            <begin position="75"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="10">
        <location>
            <begin position="141"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK12734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="33736" checksum="8C56CAD77F34E370" modified="2016-01-20" version="1">MEHTFQYAWVIPLLPLPVILSMGFGLFLIPITTKKIRRIWAFPSVLLFSIAMVFSVQLSIQQINGSSIYQYVWSWTINNDFSLEFGYLIDPLTSIMLMLITTVGILVLIYSDGYMSHDEGYLRFFIYISFFNISMLGLVTSSNLIQIFFFWELVGMCSYLLIGFWFTRPIAASACQKAFVTNRIGDFGLLLGILGFFWITGSLEFRDLFQTANNWIPNNGTTSLLTTLCAFLLFLGAVAKSAQFPLHVWLPDAIEGPTPISALILVRCNCQPVLSKVGVAMDSSHSTWFHKICMIFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PGL0</accession>
    <name>A0A0Q3PGL0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g18252v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88457.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PGL0"/>
    <dbReference type="EnsemblPlants" id="KQJ88457">
        <property type="protein sequence ID" value="KQJ88457"/>
        <property type="gene ID" value="BRADI_4g18252v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88457">
        <property type="protein sequence ID" value="KQJ88457"/>
        <property type="gene ID" value="BRADI_4g18252v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3PGL0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ88457.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="110" mass="12665" checksum="DC8EF5E874C52B77" modified="2018-03-28" version="2">MDKSKLQENEIWELRREDPALWSVRPCCVGIFFPHTTRTSTQIEVEQRHTAEEAQAAQQFLRFARPVEQLRVKQLHCIWSGPRTQHKRSHPVPFPSSSPSPPPPALVSMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PZA9</accession>
    <name>A0A0Q3PZA9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription repressor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ovate family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_3g13013v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
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                <person name="Lindquist E."/>
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        </source>
    </evidence>
    <sequence length="213" mass="23421" checksum="032FE9E51B74B68A" modified="2016-01-20" version="1">MAARSALLFALLVAALSFTATAAGDAPFIVAHKKVALSRPKPGVERLAVSLDLYNQGSATAYDVAITDDSWPKEAFELVSGEVSKTLERLDPGATASHVFVLETKAQGRFQGSPAVIKYRVPTKAVLQEAFSTPILALDILAERPPVKKFEWVKTRRKIWRAGVSCFLRCSVYLPGREPIKIWWGKSRQEETLIYLWFTVLVAGCLPGETLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RSX3</accession>
    <name>A0A0Q3RSX3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100825873</name>
        <name evidence="8" type="ORF">BRADI_1g27237v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16178.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RSX3"/>
    <dbReference type="STRING" id="15368.A0A0Q3RSX3"/>
    <dbReference type="EnsemblPlants" id="KQK16178">
        <property type="protein sequence ID" value="KQK16178"/>
        <property type="gene ID" value="BRADI_1g27237v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16178">
        <property type="protein sequence ID" value="KQK16178"/>
        <property type="gene ID" value="BRADI_1g27237v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2162691at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RSX3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043069">
        <property type="term" value="P:negative regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000008">
        <property type="entry name" value="GRAM domain containing 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666">
        <property type="entry name" value="PROTEIN VASCULAR ASSOCIATED DEATH 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666:SF1">
        <property type="entry name" value="PROTEIN VASCULAR ASSOCIATED DEATH 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="641"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="384"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="11"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="110"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK16178.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="732" mass="80818" checksum="4A5167536262B343" modified="2018-03-28" version="1">MSTLCNTFSVVPSSFSSSPGVDRPNPTAASRRIPKKAGRRHRRHSHRPRFYYSNPPSSLSLLVLLTPPTYRSAFASSLPHRPPAQPPPLHSPATTAPLSLPLAPGADGMASPTAAASPSRALAANTTAPSPASPPRRLASAPPGVDASAVSSPASAYSGDLSACDPSSPLLASRSEEYRLMFRLPPDEVLVQDFNCALQENILLQGHMYLFLHHICFYSNIFGYETKKTIPLQDVTDIRKAKTAAIFPNAVEIVAGTKRHFFGSFLARDEAYRIIVDAWEHHVSDTRLLLERQDAKSASSSDENGYVLLEEGKESKQDDDSSPLDRPANHTAAVGGSTDYVDSDINISKRFSKVPEDRTEETVASLDPFSSEPFDDDAPNVPESYTLITESKFQVPVEVLFDVLFSDGAFGFLDDLHKKCGDKEFRCSKWRLDEQGLARDVSFLHPIKIYLGAKFGTCQEVQKLRLYKNRHIVIRTSQEIGDAPYGDHFIVEGIWDVEQDSLDGNSCYLRVYINVAFSKKTIFRGKIEQSTKDECREVFSLWIKLGHDYLKQDNSSRLKDAEASTATNADVQSGATLISENPSENTVAYMASAPYESGLSTLVPPIHHHQQSIGKGSLASASQELWGSLVSYMRSGQSGPVLAVMLVAIIILMQVIVIVLLTRSPKVLMVSHEASGSSFGSYSKESLEWLQKRVSLLGEEMQMAEAHMEKMRHEFAWLKSHLERLEKLRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RU52</accession>
    <name>A0A0Q3RU52_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100834666</name>
        <name evidence="9" type="ORF">BRADI_1g29147v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316849.1">
        <property type="nucleotide sequence ID" value="XM_024461081.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316852.1">
        <property type="nucleotide sequence ID" value="XM_024461084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RU52"/>
    <dbReference type="STRING" id="15368.A0A0Q3RU52"/>
    <dbReference type="EnsemblPlants" id="KQK16536">
        <property type="protein sequence ID" value="KQK16536"/>
        <property type="gene ID" value="BRADI_1g29147v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16538">
        <property type="protein sequence ID" value="KQK16538"/>
        <property type="gene ID" value="BRADI_1g29147v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834666"/>
    <dbReference type="Gramene" id="KQK16536">
        <property type="protein sequence ID" value="KQK16536"/>
        <property type="gene ID" value="BRADI_1g29147v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16538">
        <property type="protein sequence ID" value="KQK16538"/>
        <property type="gene ID" value="BRADI_1g29147v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2402896at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RU52">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF274">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="8">
        <location>
            <begin position="546"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK16538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="807" mass="91245" checksum="131DD3ACDA137E44" modified="2016-01-20" version="1">MSSPSASASASGQPDPTPTNLSPKPLPSSSDSASATLQSSPPNPKSLAPPPIPKPNAAAGEEDCTPRMDMEFSSEQHAYDFYRRYASKAGFSVRRRYTNKSKKTGEVTSCKFACSLEGFNNRTSHISITPPSSSTSSRRTGCNAHLILRRTEHGFQVYAFQPRHNHPLFLFPPQPAALLPSPGFSLHSSSFSTQSTDDDATALPLRTRRQWEIKYGEAAALLNYLRRQSLADPSFHHAVQLDVEDKVANVFWADARMVIDYSHFGDVVAFDVLSRNCISLRHFASFVGCNSFGEPVIFGLALMYDETCESFQWLFQTFLHAMSGQAPKTFFSHQDTVITKAVSLAMPCTSHAICAWHVKHAATRNVNRLAKGHCDFIKEFKACINNYDEEAEFLASWEAMINKYDLRDNVWLHKLFQEKEKWARPYAKGIFSAGMSTRLNERLNSDVRDHLRAEVDIVLFLRHLQKVIKDRRYKELEFEYSSRLKLPCLKIRAPMLTQASEVYTSMIFQLFQEEYEEFQSAYIVNRDEGGPCREYIVSIVEKEKQYTVYGSSMEQTVSCSCRKFETIGFLCSHALKILDTMDIKYIPDRYIMRRWTKYARCLTSPEVLGQTVQAERSEEISNRYQHMCPKYVRLVARASECEESYRVLDQFWGELSEKVEEILQKQSIGTSVTQPDVHNLKMSLSSITNGTGSENVLDKSCSTPARIVKKKDKKSKGHPVNYIEKGLRKKQKVHLEQPAVQYAVLNASAQSENAMFQDLEAPPNMSQGTDLSSSMGDINYEETRSGLSPTFTPELDFCGLSQFSGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CER1</accession>
    <name>A0A2K2CER1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g01035v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CER1"/>
    <dbReference type="EnsemblPlants" id="PNT60518">
        <property type="protein sequence ID" value="PNT60518"/>
        <property type="gene ID" value="BRADI_5g01035v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60518">
        <property type="protein sequence ID" value="PNT60518"/>
        <property type="gene ID" value="BRADI_5g01035v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CER1"/>
    <dbReference type="OrthoDB" id="1002684at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT60518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="65" mass="7505" checksum="8AB2F1FEAFA0B823" modified="2018-03-28" version="1">MRIMLNTGVQEIRFLVHPSVYKLTTRSEHGCLVRSHIDLSTNMSKLKNITPPTNILNLEQNRQDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CFZ8</accession>
    <name>A0A2K2CFZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100832523</name>
        <name evidence="8" type="ORF">BRADI_5g08550v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CFZ8"/>
    <dbReference type="STRING" id="15368.A0A2K2CFZ8"/>
    <dbReference type="EnsemblPlants" id="PNT60952">
        <property type="protein sequence ID" value="PNT60952"/>
        <property type="gene ID" value="BRADI_5g08550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60952">
        <property type="protein sequence ID" value="PNT60952"/>
        <property type="gene ID" value="BRADI_5g08550v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="262529at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CFZ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16273">
        <property type="entry name" value="SNM1A-1C-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12650:FF:000001">
        <property type="entry name" value="DNA cross-link repair 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000010">
        <property type="entry name" value="DNA cross-link repair 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF36">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN SNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="7">
        <location>
            <begin position="356"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="103"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="103"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT60952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="556" mass="61869" checksum="19F449B8D6795052" modified="2018-03-28" version="1">MDTDITGDAETDLPPPVSDDLDDNGFPILPPTAAATSSFADDFYRSGIDWSSLQLQAAPPHQRNPAAGTKAKCGGPLVQRSLFQAWGIEKPPREEAAQGVRAAAGVSSSSASHSGTWSGRKRRWGGDSEENGVAKKPATCPFYKKIPGTPFTVDAFRYGAVEGCSAYFLSHFHYDHYGGLTKKWCHGPIYCTALTARLVKMLLSINSEYICPLELNTEYVIDGVTVTLLEANHCPGAALIHFRLSDGKTYLHTGDFRASKSMQSHPLLQTGRINLLYLDTTYCNPKYKFPPQEDVIDFVVRTAQRYLKKQPKTLIVVGAYSIGKENVYLAISQALEVPIYTDASRRRILHSFGWPDLSKRISSCDQSSPLHVMPLASVQHEKLTKYLETLNQRFLAVLAFRPTGWTFSEAAGKELDLIKPSSRGRVTIYVASRWYMRSSQIFSRRLGLFILLCAGKDMLKEITQSTACIMVRAGDLRANVLVSLEALVAYFTYISVRCQQCLRDDGTAFGQCCLIDCQLLLLLEVDDRLFLRFLRHCLVAHFLVCGCAREAMRMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CLY5</accession>
    <name>A0A2K2CLY5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_4g10675v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CLY5"/>
    <dbReference type="EnsemblPlants" id="PNT63042">
        <property type="protein sequence ID" value="PNT63042"/>
        <property type="gene ID" value="BRADI_4g10675v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63042">
        <property type="protein sequence ID" value="PNT63042"/>
        <property type="gene ID" value="BRADI_4g10675v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CLY5"/>
    <dbReference type="OrthoDB" id="599337at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1201">
        <property type="entry name" value="OS02G0301800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="239"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="405"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="523"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="882"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT63042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="904" mass="101145" checksum="5D87C159E9BDBA61" modified="2018-03-28" version="1">MDITTGAMASLLPKLLQLLEEEYKLHKGIKKEVQSLLKELASMHAALHEVGRVPREQLDEQVKIWADDVRELSYNMEDVVDTFLVRAEGSEPAADLDGFRSLIKKMGNLLKMRKVRHQISDMIKDIKDQVHDAAARRDRYRVDSVVANSDATAGTLDPRLSAMYNKLTELVGIGEPRDEIIKRLTVKNDGVETVSIAGFGGLDKTALAKAVYDSLKAEFHCTAFVSVSQNPEITRIFKKILHMLDEDKYLIVIDDIWKIQSWKTIKYGLVENRCGSRIITTTRDFAVADQVGGSYKLKPLSLESSKELFYGRIFGSEEKCPQELTEVSLKILKKCGGVPLAVITMASLLTVDSTIANPREWDQVCDSIGSGLGNNPGVKDMRTILSLSYYNLPSHLRTCLLYLPIYPEDYVVPRDRLIWKWIAEGFIPENSKDGRSLFEVGVSYYNELISSCMIQPAEYFQGCHLHDMMLELICSLSSEENFVSILDHIEDSTSPQSNSRRISLQNKRENHQTTPPFCMRVTQVRSVTVFPPAIDLMPPLSSYGVLRVLDLSGCYLGGSGHIRLKYIGNLLHLRYLGLANTKICELPSSIGRLQFLQVLDIRSNSQLPALPPSIIKLKRLLCLQSDTYGMAIFLDGFGNLTSMEVLTGIPASLNIVKELCNLARLRVLEIFFTYDQSLEVEGAFVESVCKLPNIQSLTVQGSFESMDLFSGRWVPSQHLRIFDWYNAGVFSTLPAWIRRYPSHLSNLSELIIKVKNLQEEDLRVLGMLPSLSILGLWSTHQTERLLVIGADAFHCLATFIVHLTPPAQIIFGQGVLPRAEEVGFNFGVRLAKDEGNDDFDSGLGNLLSLQRAFLRICCGGVTVGVARKAEAVLRHQVDVHPNHPEATISTEPSIPQDADEDQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQ89</accession>
    <name>A0A2K2CQ89_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC104584644</name>
        <name evidence="13" type="ORF">BRADI_4g25745v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237950.1">
        <property type="nucleotide sequence ID" value="XM_010239648.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQ89"/>
    <dbReference type="EnsemblPlants" id="PNT64191">
        <property type="protein sequence ID" value="PNT64191"/>
        <property type="gene ID" value="BRADI_4g25745v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584644"/>
    <dbReference type="Gramene" id="PNT64191">
        <property type="protein sequence ID" value="PNT64191"/>
        <property type="gene ID" value="BRADI_4g25745v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584644"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000404">
        <property type="entry name" value="RING-H2 finger protein ATL72-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044602">
        <property type="entry name" value="ATL10/ATL72-79-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46905">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46905:SF21">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="PNT64191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="201" mass="20035" checksum="86C6B9B82EACD40F" modified="2018-03-28" version="1">MGARRLSGGGADTTAPPLVTPAAAAASAGGTFTTTTTNNSNSSGGGNGGSFDANMVIILAALLCVLIFALGLNSLVRCCLHCARRLSSSSSAPSSSSSPAPASAGLKRKALRRIPVEVYGASNGVTAEGRAAAAECAICLGEFAGGEKVRVLPRCRHGFHVRCIDAWLAAHSSCPNCRASLLVPDPGAAAGAGEDGARGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CR81</accession>
    <name>A0A2K2CR81_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g29901v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CR81"/>
    <dbReference type="EnsemblPlants" id="PNT64534">
        <property type="protein sequence ID" value="PNT64534"/>
        <property type="gene ID" value="BRADI_4g29901v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64534">
        <property type="protein sequence ID" value="PNT64534"/>
        <property type="gene ID" value="BRADI_4g29901v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CR81"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT64534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="17039" checksum="131763099E1AF4FD" modified="2018-03-28" version="1">MGAILLPAGTIHDIDKRCRAFFWTGDGRCPFCVRTETTSHMLLHCHSVQPIWSALQALHYDASSCESLEELLTARHPPDKVHSTVLLAIICNIWKRRNAMVFNNLIKLPHLVIERCSNDIALWSHKCGSSAPKALMNDWSIMLTHLARWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CRS8</accession>
    <name>A0A2K2CRS8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g32300v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CRS8"/>
    <dbReference type="FunCoup" id="A0A2K2CRS8">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT64732">
        <property type="protein sequence ID" value="PNT64732"/>
        <property type="gene ID" value="BRADI_4g32300v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64732">
        <property type="protein sequence ID" value="PNT64732"/>
        <property type="gene ID" value="BRADI_4g32300v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CRS8"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CRS8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF358">
        <property type="entry name" value="OS09G0460300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="151"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT64732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="390" mass="41711" checksum="E4E827D17FBC6866" modified="2018-03-28" version="1">MPFFSMAIRSGANIWAGWAATQPYRRLAAHRRSLLQLPYLSFRFSPQIKCPGVWRSRARSSTSHDNGAPPVTATEPEAQDEVLLESPAQFRIYKSGKIDRLNERTLSPTGLDEATGVTSKDVVLDADTGVSVRLYLPMLKEPAASTKLPVLVYFHGGAFLIGSAGDATYHSYVNALAAAAGVLVVSADYRLAPEHPLPAAYDDSWAALQWAAVSAQDDWITQYGDTSRLFLAGDSAGANIVHDMLMRAASDNDGGEPRIEGAILLHPWFSGSTAIEGEPPAAAMITGMLWSYACPGAVGGADDPRMNPLAPGAPALEKLGCVRMLVTAGLKDGLAARDRAYYDALVASGWRGDAAWLESEGEGHVFFLEKPGCENAKQLMDRVVAFIAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CXN5</accession>
    <name>A0A2K2CXN5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100846522</name>
        <name evidence="6" type="ORF">BRADI_3g17050v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317801.1">
        <property type="nucleotide sequence ID" value="XM_024462033.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317802.1">
        <property type="nucleotide sequence ID" value="XM_024462034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CXN5"/>
    <dbReference type="STRING" id="15368.A0A2K2CXN5"/>
    <dbReference type="EnsemblPlants" id="PNT66795">
        <property type="protein sequence ID" value="PNT66795"/>
        <property type="gene ID" value="BRADI_3g17050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846522"/>
    <dbReference type="Gramene" id="PNT66795">
        <property type="protein sequence ID" value="PNT66795"/>
        <property type="gene ID" value="BRADI_3g17050v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="592605at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CXN5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001729">
        <property type="term" value="F:ceramide kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF37">
        <property type="entry name" value="OS08G0152700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="5">
        <location>
            <begin position="103"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT66795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="526" mass="58066" checksum="AB0758F4D31AA051" modified="2018-03-28" version="1">MGSRSFVRSPVSCSAVNEFFTCSSHCSRNYYQCSNTMKSQRAQVGQTLLPRKLRKSTRWQTALFTQRRIVSNCCSDLSTTYTEEVPNYLALNVLQDQSNTGQDNIRKVLVILNPNSGFRSSRAVFYKKVQSTLKLSGFTMKVVETAYAGHAKVLASTVDLSTCPDGIICVGGDGVVNEVLNGLLGRDDLKEALQLPIGIVPAGSDNSLVWTVLGIRDPVSAATALAKGGFTPIDVFAVKWIQAGVTHFGLTASFCGFVADVLQLSENFRLQLGPFRYVIAGLLKFLSLPQYRFEVDYLPSPGRNPELKSPTQNCCDQLSDGSKVKRGSTEDNWVMRKGEFLGILVCNHFCKPAQGLLSPVMAPKAQHDDGSLDLILIHGSGRLRLFCFFVAYQFCWHLLLPFVEYVKVKQVKIRPVGGTHNGCGVDGELLQGEGQAEWQCSLLPAQVPSRRRGAMDVSLLAYRCFIAFYSHLQYLPCKQVKIRPVGNTHNGCGVDDELLQAETKWNRTIGGSGESMHCQMFLRPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D031</accession>
    <name>A0A2K2D031_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100834726</name>
        <name evidence="20" type="ORF">BRADI_3g29917v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="15368.A0A2K2D031"/>
    <dbReference type="EnsemblPlants" id="PNT67640">
        <property type="protein sequence ID" value="PNT67640"/>
        <property type="gene ID" value="BRADI_3g29917v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67640">
        <property type="protein sequence ID" value="PNT67640"/>
        <property type="gene ID" value="BRADI_3g29917v3"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="expression patterns" value="baseline"/>
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        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="term" value="P:DNA-templated transcription"/>
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        <property type="term" value="P:megagametogenesis"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
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        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
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    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
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    </dbReference>
    <dbReference type="InterPro" id="IPR009674">
        <property type="entry name" value="Rpa2_dom_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="26"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
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            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
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            <end position="520"/>
        </location>
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    <feature type="domain" description="DNA-directed RNA polymerase I subunit RPA2" evidence="19">
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            <begin position="574"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="14">
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            <begin position="689"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="15">
        <location>
            <begin position="1076"/>
            <end position="1186"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D092</accession>
    <name>A0A2K2D092_BRADI</name>
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        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">BRADI_3g30712v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_024461420.1"/>
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        <property type="protein sequence ID" value="PNT67697"/>
        <property type="gene ID" value="BRADI_3g30712v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112271675"/>
    <dbReference type="Gramene" id="PNT67697">
        <property type="protein sequence ID" value="PNT67697"/>
        <property type="gene ID" value="BRADI_3g30712v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:112271675"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47069">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47069:SF11">
        <property type="entry name" value="OS04G0275550 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="13"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT67697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="282" mass="32230" checksum="0A198C7AA8CBD312" modified="2018-03-28" version="1">MEPIKVDFVKAVWDATAARIFCECATKQVQLGNRPTKFLSSTGYKNLVADFNAQTGRNYERRQLKNRWDDLKAIHSAWVFYKNKATRLGWNEDTQTIIADEEQWAELIKIHKPIKAFRKGPPDNLELMHIMFDKANVDGTTSIMLGVEEVVAIQVEEDVIDLDEEETSTVSPWLKTRPKRPSSAIPCSPSNPKKANMSNEFQMFVDHVVTESSSNSTTTEAKEIAAIMEEVPKCGASELSDEHYIATKLFAKSTNRSFFLSMKTNEGQLNWLKRQYEDRKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D9W8</accession>
    <name>A0A2K2D9W8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106866036</name>
        <name evidence="7" type="ORF">BRADI_2g22797v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754045.1">
        <property type="nucleotide sequence ID" value="XM_014898559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D9W8"/>
    <dbReference type="EnsemblPlants" id="PNT71080">
        <property type="protein sequence ID" value="PNT71080"/>
        <property type="gene ID" value="BRADI_2g22797v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106866036"/>
    <dbReference type="Gramene" id="PNT71080">
        <property type="protein sequence ID" value="PNT71080"/>
        <property type="gene ID" value="BRADI_2g22797v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:106866036"/>
    <dbReference type="OrthoDB" id="786283at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF19">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="6">
        <location>
            <begin position="11"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT71080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="177" mass="19560" checksum="291FE4A503103ADB" modified="2018-03-28" version="1">MSQHNVNLVCWNVRGLNNPSHRSAVCDLIRDTHATIICIQETKLQVVNDRLIRDLLGPCFSANFAVLPAAGTRGGMILAASEDFFTISDVHLSAHSITVTVTMRSEGASWSLTSVYGQQGDPEKLLFIEELKLLEPVVKSEWILLGDFNVITKATDKNNTNINRRLIGKFRGALDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DA36</accession>
    <name>A0A2K2DA36_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g23667v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DA36"/>
    <dbReference type="STRING" id="15368.A0A2K2DA36"/>
    <dbReference type="EnsemblPlants" id="PNT71145">
        <property type="protein sequence ID" value="PNT71145"/>
        <property type="gene ID" value="BRADI_2g23667v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71145">
        <property type="protein sequence ID" value="PNT71145"/>
        <property type="gene ID" value="BRADI_2g23667v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DA36"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033865">
        <property type="entry name" value="Ataxin-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159:SF0">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT71145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="166" mass="18548" checksum="0B2901ED3A2C85EB" modified="2018-03-28" version="1">MSNRSLYPAPENLPRFTLAPHLDNLRVSGVHIYAVRGTLPTKCPNDYNDFGQWLSPDEASNIMRSRYQAQKKQHSNSAIEMASRHYHMVAIAASLRGVQVEVQSEQPYKQEDVSTKQEKNAADLELAIAISLMPFEATVASCHPAQGEVCNMEHDSSKCEDSTKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DAN7</accession>
    <name>A0A2K2DAN7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g26397v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        </location>
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    <evidence type="ECO:0000259" key="1">
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    </evidence>
    <evidence type="ECO:0000313" key="2">
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            <dbReference type="EMBL" id="PNT71344.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="181" mass="21290" checksum="DC5645E382F09F8C" modified="2018-03-28" version="1">MKVAGRGRQIFVAEALVDRAWVKWVKPDLSSLAIDEFLLIWDIVEGWALSDEDDVLRWKWEGSVDRLRRRKMPHPPCCPLCDQVEENINHLLLGCVVAWQVWLIVLGRWQRQDWLPGVLDRLETWWPGRVVVARKDRRNLHTAVNLVYWCIWKHRNAVVFDGVTPSVTHIIREIGREGDAW</sequence>
</entry>
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        <name evidence="3" type="ORF">BRADI_2g27197v3</name>
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_2g27197v3"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="F-box protein AT5G49610-like beta-propeller" evidence="2">
        <location>
            <begin position="129"/>
            <end position="173"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT71416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="187" mass="21380" checksum="548D62FFA314486D" modified="2018-03-28" version="1">MIPEIGRDRRLKMDFPSSPAAVVAKVLDDDDLLREILLRVGFPTTLVRAALVCTRWYHHAADRAFLRRFRQRHPPRLLGLYVGDVFIPHTLEFLPRFVPLLTKTQELELATVLCRMATFSLDTIHGMSIKECRNGSIFISGFRNHKYAYEVHSPLFPERGVAVLPPPPPHAVTDDAHNLLLLPSQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DCV0</accession>
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            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">BRADI_2g39435v3</name>
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_2g39435v3"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="PNT72104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="106" mass="11596" checksum="759F1777A0B0DC4E" modified="2018-03-28" version="1">MCVQIQKSKPRLVCGLVGRQNSLRSRNGDREPHRDAAAISAAALRISISGRPPSLAISGRPCFCSLIQDSCKRIICECDGLVLEPLVYPNSSLQVERNTLVLFCAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGJ3</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
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        <property type="gene ID" value="BRADI_2g58016v3"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="PNT73406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="57" mass="6432" checksum="DF324FBBE0DC0220" modified="2018-03-28" version="1">MCIEFPHGVVISSASQDSQTKDQRDMLSKYIPTRRTFAMNVAKAKSMPCDVMSRPHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DLM3</accession>
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        </alternativeName>
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    <organism evidence="10">
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DLM3"/>
    <dbReference type="STRING" id="15368.A0A2K2DLM3"/>
    <dbReference type="EnsemblPlants" id="PNT75180">
        <property type="protein sequence ID" value="PNT75180"/>
        <property type="gene ID" value="BRADI_1g28380v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75180">
        <property type="protein sequence ID" value="PNT75180"/>
        <property type="gene ID" value="BRADI_1g28380v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DLM3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="130"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT75180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="343" mass="38119" checksum="2B5D08260133B414" modified="2018-03-28" version="1">MECFRRVNPFRACAGLRVLGYLMLALVAAIVAVSYYAVVVYAWGPLLLGGGGGSVAAAAIVLAAFHLLLIMMLWCYLMVVFTDPGAVPENWRHAAEEDDMDESNTRTISNDVATDIVNPPLFTSEGQGNASRYCSRCQNGKPPRCHHCSICDRCVLKMDHHCVWVVNCVGARNYKYFLLFLVYTFLETVLDTLVLLPSFITFFRDESRRSSSASDVAILFLAFVLNLAFALSLLIFIGMHTSLVASNTTSIESIRLAIIFVGSWLVLLACPNDMLCVGSNLLQVYERKKTVSWQYDLGWRKNLEQVFGTKKLFWFLPVHSSEDLHNIPALQGLGFPARPGAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQ31</accession>
    <name>A0A2K2DQ31_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminotransferase-like plant mobile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g47682v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQ31"/>
    <dbReference type="EnsemblPlants" id="PNT76389">
        <property type="protein sequence ID" value="PNT76389"/>
        <property type="gene ID" value="BRADI_1g47682v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76389">
        <property type="protein sequence ID" value="PNT76389"/>
        <property type="gene ID" value="BRADI_1g47682v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DQ31"/>
    <dbReference type="OrthoDB" id="693469at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835:SF63">
        <property type="entry name" value="AMINOTRANSFERASE-LIKE PLANT MOBILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="31059" checksum="E6780851FED7A93D" modified="2018-03-28" version="1" fragment="single">MYKLHLLPKINLHFSAWLLQRINTSTMSLDPCDGSSFDLTPESVHEVFELPIGNDAVVSESTIFSESLDDLKFAEVYLLKPLSEESSKIEIECFQIAFVIYVVSNLLAPTAKHDYVTLDFLGSPANISTISSFNWCEFVLKHIVLAANKLNSDIASGASSIHMGGCHLFLQVFYLDHIDLGVYNKPKLVSPRIGLFDYESLKKMIDHLSAVNGIESSFFPPVSLAQDKQDKKDATSNSAAATRRQGNHLNVLTKTHASNTALSCSNPGPNDFARHLQVNPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQ35</accession>
    <name>A0A2K2DQ35_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104581629</name>
        <name evidence="3" type="ORF">BRADI_1g47636v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010227962.1">
        <property type="nucleotide sequence ID" value="XM_010229660.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312330.1">
        <property type="nucleotide sequence ID" value="XM_024456562.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312331.1">
        <property type="nucleotide sequence ID" value="XM_024456563.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312332.1">
        <property type="nucleotide sequence ID" value="XM_024456564.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312333.1">
        <property type="nucleotide sequence ID" value="XM_024456565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQ35"/>
    <dbReference type="EnsemblPlants" id="PNT76385">
        <property type="protein sequence ID" value="PNT76385"/>
        <property type="gene ID" value="BRADI_1g47636v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581629"/>
    <dbReference type="Gramene" id="PNT76385">
        <property type="protein sequence ID" value="PNT76385"/>
        <property type="gene ID" value="BRADI_1g47636v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104581629"/>
    <dbReference type="OrthoDB" id="1743609at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF5">
        <property type="entry name" value="OS05G0421675 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044576658" description="Reverse transcriptase zinc-binding domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="2">
        <location>
            <begin position="156"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT76385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="282" mass="32670" checksum="360CCD5B4ECD6E4A" modified="2018-03-28" version="1">MGSRFLAVLLQCWSREFAAFCRCCFERRLHGFVRSVPKLDDGVGSFWWRDLVGLIAGYKEVARCLPGGGSTISFWKDAWDSDPFMTRFSRIFSFAINKEITLLEFHSGEDRYDLFALPLSTLTSDELQHIIAILHSAALDQTDQDLWICRLGSGAFSTKLFYQHHFPALHRRLKSTWIWKNNCLPKIKGFAWLLYRDRVNTRDMMDRRNCNRDGVLSCALCDSGSRETRDHLFFTCFSAECWLRIGVNWSPLPSEPSLPNRLHLVKAHVPVRIHREVFLSMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DR14</accession>
    <name>A0A2K2DR14_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g52116v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DR14"/>
    <dbReference type="EnsemblPlants" id="PNT76718">
        <property type="protein sequence ID" value="PNT76718"/>
        <property type="gene ID" value="BRADI_1g52116v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76718">
        <property type="protein sequence ID" value="PNT76718"/>
        <property type="gene ID" value="BRADI_1g52116v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DR14"/>
    <dbReference type="OrthoDB" id="682412at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT76718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="69" mass="7594" checksum="BAD63A1C4A98DA88" modified="2018-03-28" version="1">MGRKSSRAASSLFILTLWSIWKERNNKIFNRLHRPAPSLISAIKAEASLWGLVDTSGLGALVSRSDDVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DRX3</accession>
    <name>A0A2K2DRX3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_1g56930v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DRX3"/>
    <dbReference type="FunCoup" id="A0A2K2DRX3">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77029">
        <property type="protein sequence ID" value="PNT77029"/>
        <property type="gene ID" value="BRADI_1g56930v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77029">
        <property type="protein sequence ID" value="PNT77029"/>
        <property type="gene ID" value="BRADI_1g56930v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DRX3"/>
    <dbReference type="OrthoDB" id="5788137at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DRX3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000017">
        <property type="entry name" value="3-oxo-5-alpha-steroid 4-dehydrogenase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF35">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="8">
        <location>
            <begin position="357"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT77029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="472" mass="51587" checksum="8BA7340F8323B0F5" modified="2018-03-28" version="1">MIIAVSVSKRCESVRIIVQCPNQMPSYMIKADDHKLCPPSRSGMKHLAKHLNSQRPIHEYLEKNLYCTDISSTSTYLFGYSLERDASACQLHRHHGQENARCREIDTDPTASGRPLELRSSGQPRPARVRAAGGTYVHEFLAVDFAQFPHNRQMRNARSTSASAPQPHDLFFFHTQTDGLDLEGDRRALVEMWQLLPSALLYPAPASAFVTAMSVLSSASLASAGLSELRGQHMAYSKFWHVVASGSTQKNPGGGGERLLSSRDGMLVAYAPALVAAAASFALPGALEEGLRAQLLAGALAVHFLKRVLEVLFVHRYSGSMPLNTALMISSSYLLSTITMIYSLHLAAGLPEPSINLLYPGVLVFTVGIAGNFYHHYLLSRLRKGSGTTSEGDKSYKIPTGGLFGLVACPHYLFEIAGFFGFAMISQTVYALAMASGTAAYLTGRSCATRRWYASKFEEYPARIKALVPYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GL57</accession>
    <name>I1GL57_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100846650</name>
        <name evidence="6" type="ORF">BRADI_1g02560v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559189.1">
        <property type="nucleotide sequence ID" value="XM_003559141.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GL57"/>
    <dbReference type="STRING" id="15368.I1GL57"/>
    <dbReference type="EnsemblPlants" id="KQK12268">
        <property type="protein sequence ID" value="KQK12268"/>
        <property type="gene ID" value="BRADI_1g02560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846650"/>
    <dbReference type="Gramene" id="KQK12268">
        <property type="protein sequence ID" value="KQK12268"/>
        <property type="gene ID" value="BRADI_1g02560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846650"/>
    <dbReference type="eggNOG" id="ENOG502R1VN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108204_1_0_1"/>
    <dbReference type="OMA" id="GWCSGKN"/>
    <dbReference type="OrthoDB" id="676007at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF10">
        <property type="entry name" value="HARPIN-INDUCED PROTEIN 1 CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="5">
        <location>
            <begin position="77"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK12268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="209" mass="22518" checksum="2A47230BF84ACAA5" modified="2012-06-13" version="1">MGFWERDHEWDWKKTAIWAAVLLLALGVLALLIVAFAVASPPKATADDALLQRFTLSSTPSSPTPNSTISYNATVAVSLYNPNIHRAISYGAMAAAFSFNGTRFDDQATVAAFEQGARKTTTVRVTVGGADRAVRLSAAGVAEFGREKGDGRFMVEVRMESVMRYKGRKTDCPVVVVCPLLLQLVDPAVAATAFQKTKCTILRAKKSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLY6</accession>
    <name>I1GLY6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="11">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100827255</name>
        <name evidence="13" type="ORF">BRADI_1g04850v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family. PP-2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="11"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564197.1">
        <property type="nucleotide sequence ID" value="XM_003564149.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLY6"/>
    <dbReference type="FunCoup" id="I1GLY6">
        <property type="interactions" value="1496"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GLY6"/>
    <dbReference type="EnsemblPlants" id="KQK12605">
        <property type="protein sequence ID" value="KQK12605"/>
        <property type="gene ID" value="BRADI_1g04850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827255"/>
    <dbReference type="Gramene" id="KQK12605">
        <property type="protein sequence ID" value="KQK12605"/>
        <property type="gene ID" value="BRADI_1g04850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827255"/>
    <dbReference type="eggNOG" id="KOG0371">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_8_1_1"/>
    <dbReference type="OMA" id="EGYNWGQ"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GLY6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="12">
        <location>
            <begin position="112"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK12605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="307" mass="35234" checksum="0F2E4A2B86F164D0" modified="2012-06-13" version="1">MSSPHGGLDDQIERLMQCKPLPEAEVRALCEKAKEILMEESNVQPVKSPVTICGDIHGQFHDLAELFRIGGKCPDTNYLFMGDYVDRGYYSVETVTLLVSLKVRYPQRITILRGNHESRQITQVYGFYDECLRKYGNATVWKTFTDLFDYFPLTALVESEIFCLHGGLSPSIETLDNIRNFDRIQEVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDISEQFNHTNNLRLIARAHQLVMEGFNWAHEQKVVTIFSAPNYCYRCGNMASILEVDDCREHTFIQFEPAPRRGEPDVTRRTPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMZ9</accession>
    <name>I1GMZ9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100844619</name>
        <name evidence="2" type="ORF">BRADI_1g07710v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559040.1">
        <property type="nucleotide sequence ID" value="XM_003558992.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMZ9"/>
    <dbReference type="FunCoup" id="I1GMZ9">
        <property type="interactions" value="424"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GMZ9"/>
    <dbReference type="EnsemblPlants" id="KQK13051">
        <property type="protein sequence ID" value="KQK13051"/>
        <property type="gene ID" value="BRADI_1g07710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844619"/>
    <dbReference type="Gramene" id="KQK13051">
        <property type="protein sequence ID" value="KQK13051"/>
        <property type="gene ID" value="BRADI_1g07710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844619"/>
    <dbReference type="eggNOG" id="ENOG502S0TF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034795_1_0_1"/>
    <dbReference type="OMA" id="SVSYACH"/>
    <dbReference type="OrthoDB" id="655446at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36072:SF2">
        <property type="entry name" value="OS01G0531000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36072">
        <property type="entry name" value="OS01G0541600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK13051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="377" mass="40661" checksum="FF5E4121E8BE385E" modified="2012-06-13" version="1">MGKKRGNSAPSSASRATNQALSLREESCGKTQADAASLLRVQHLQRLAAWASGEAGVSPVGALLGHRLATKAEAAGIPLGASTFLCQRCESVLQPGFNCTIRIKNNKRNAKRCKKSNSCQNSVAYVCHFCGSQNLIRGSGKGIVKGLLSSRKPYNMDQTGIMSKGNRLDHSFPAVAQVESSRLKKSTLEQYDHVGVSKSNLPEDSSMAEGIVSSLVCHSQLAASTVQVDITPKIKVEITNNKDMNDIQPVSSEKIGPCESDVTSQAEFRVTSKFVTPQKNKLADVAAPKDSAEPLKKRSTLNNKGESCVSVTGKAPIKLTSNDSGKNTMSAPRDSSQMAGSSRKRARKGWTTLKQIAEKDEFERKEKIVNFVIPFFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1GN68</accession>
    <name>I1GN68_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100821755</name>
        <name evidence="9" type="ORF">BRADI_1g08301v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559369.1">
        <property type="nucleotide sequence ID" value="XM_003559321.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GN68"/>
    <dbReference type="EnsemblPlants" id="KQK13143">
        <property type="protein sequence ID" value="KQK13143"/>
        <property type="gene ID" value="BRADI_1g08301v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821755"/>
    <dbReference type="Gramene" id="KQK13143">
        <property type="protein sequence ID" value="KQK13143"/>
        <property type="gene ID" value="BRADI_1g08301v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821755"/>
    <dbReference type="eggNOG" id="KOG0765">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_3_3_1"/>
    <dbReference type="OMA" id="GPSGILM"/>
    <dbReference type="OrthoDB" id="250329at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080:SF3">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="22"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="125"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="232"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK13143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="34991" checksum="ED365776E86783C7" modified="2012-06-13" version="1">MDTSTRAAKIPSLYHQTEINWENLDMTKFYVVGAGMFSCVTVALYPVSVIKTRMQVASGEAMRRNALATFKNILKVDGVPGLYRGFGTVITGAIPARIIFLTALEKTKATSLKLVEPLKLSESMEAALANGLGGLTASLCSQAVFVPIDVVSQKLMVQGYSGHVRYKGGLDVAQKIIKADGPRGLYRGFGLSVMTYAPSSAVWWASYGFSQRIIWSALGHWHDKEDTPGQLKIVGVQATGGMIAGAVTSCVSTPLDTIKTRLQVNQNKPKAGEVIRRLIAEDGWKGFYRGLGPRFFSSSAWGTSMIVCYEYLKRVCAKVEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPH0</accession>
    <name>I1GPH0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100843012</name>
        <name evidence="8" type="ORF">BRADI_1g12190v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561175.1">
        <property type="nucleotide sequence ID" value="XM_003561127.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPH0"/>
    <dbReference type="FunCoup" id="I1GPH0">
        <property type="interactions" value="299"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13743">
        <property type="protein sequence ID" value="KQK13743"/>
        <property type="gene ID" value="BRADI_1g12190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843012"/>
    <dbReference type="Gramene" id="KQK13743">
        <property type="protein sequence ID" value="KQK13743"/>
        <property type="gene ID" value="BRADI_1g12190v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843012"/>
    <dbReference type="eggNOG" id="KOG1237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009313_4_2_1"/>
    <dbReference type="OMA" id="FMICAKW"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17416">
        <property type="entry name" value="MFS_NPF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="515"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="558"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK13743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="588" mass="64345" checksum="D83417FC1D4917BB" modified="2012-06-13" version="1">MRSGGGGGREDDEAGQKLKSMDVDKMENGADDSPRPLVKYHGWKAMPFIIGNETFEKLGTLGTSANLLVYLTQVFHMQSVDAATLLNGLNGTTSLAPIVGAFLSDAYLGRYLALAIASVASLIGMFFLTLTAGADSLHPPECGAGEVCQKATSYQFAVLFISFAFLVIGSAGIRPCSMPFGADQFDPHTESGKRGINSFFNWYYFTFTSAMLVSATVIIYVQSNVSWPIGLGIPTALMFLACVLFFMGTKLYVRVIPEGSPFTTIVQVFAAAVSKRSLKQAKDPKQDLFDPPHTSNVVSKLAHTDQFRCLDKAAMVASPEEVRPGGAAPVNPWRLCTVQQVEEVKCLIRIVPVWSTGIIYYVAVVQQSTYVVFSALQSDRRLGSSFHVPAASFTVFAMLAQTLWIPIYDRILLPRLRKVTGKDEGFTLLQRQGIGIALSTVAMVISAIVEDRRRDIALNQPVIGTTQTGGGISAMSSFWMVPQLMILGLSEAFNLISQIEFYYKEIPEHMRSVAGALAFCNLALGNYLSGFLTTIVHQTTGAGQNWLAQDLNKGRLDLFYWMIAGIGVFNFVYFMICARWYRFKGASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQN2</accession>
    <name>I1GQN2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100827348</name>
        <name evidence="1" type="ORF">BRADI_1g15750v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562356.1">
        <property type="nucleotide sequence ID" value="XM_003562308.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQN2"/>
    <dbReference type="STRING" id="15368.I1GQN2"/>
    <dbReference type="EnsemblPlants" id="KQK14370">
        <property type="protein sequence ID" value="KQK14370"/>
        <property type="gene ID" value="BRADI_1g15750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827348"/>
    <dbReference type="Gramene" id="KQK14370">
        <property type="protein sequence ID" value="KQK14370"/>
        <property type="gene ID" value="BRADI_1g15750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827348"/>
    <dbReference type="eggNOG" id="KOG1947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_913321_0_0_1"/>
    <dbReference type="OMA" id="NINPGEP"/>
    <dbReference type="OrthoDB" id="1108417at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905761">
        <property type="term" value="F:SCF ubiquitin ligase complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF12">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK14370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="33364" checksum="5C6F19B33EDD9DC3" modified="2012-06-13" version="1">MASPPVRQWGELQHDLLVSIMSRVGSPDLLSGGAPRACSAWWGAARDPLAWRRVDLRDWTALTSARRAAGTGGTSRRVSVQDDLAGVLEIAARRADGRMEAVLLPEFADEEHLLFLAERSLNLHYFSFPTTCMTYDQFCKVIGKLQYLRGMAVDESLINYDVLLHVYQCCPNFLELKVFAVYVDEDMASIICDCLPRLKKLEIPNSDMSCAAIIKFLDCLEELEYMDISGYETSVISSSVLHKASRLKVFVWNSKFELGEFKDCSNCGEHSINPQEPCKCTIDHKVMDWLAGPLQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSK0</accession>
    <name>I1GSK0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable 6-phosphogluconolactonase</fullName>
            <ecNumber evidence="6">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100838155</name>
        <name evidence="8" type="ORF">BRADI_1g22050v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 6">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="6"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562731.1">
        <property type="nucleotide sequence ID" value="XM_003562683.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562732.1">
        <property type="nucleotide sequence ID" value="XM_003562684.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSK0"/>
    <dbReference type="STRING" id="15368.I1GSK0"/>
    <dbReference type="EnsemblPlants" id="KQK15344">
        <property type="protein sequence ID" value="KQK15344"/>
        <property type="gene ID" value="BRADI_1g22050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838155"/>
    <dbReference type="Gramene" id="KQK15344">
        <property type="protein sequence ID" value="KQK15344"/>
        <property type="gene ID" value="BRADI_1g22050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838155"/>
    <dbReference type="eggNOG" id="KOG3147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053947_0_0_1"/>
    <dbReference type="OMA" id="YQLFEFE"/>
    <dbReference type="OrthoDB" id="432544at2759"/>
    <dbReference type="UniPathway" id="UPA00115"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000009">
        <property type="entry name" value="Probable 6-phosphogluconolactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF8">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="18"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK15344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="277" mass="30584" checksum="F5893F5B4FA86448" modified="2012-06-13" version="1">MAASYEPKMNGEIRIFESSDEIATDLAEYISQVSEISVKERGYFAIALSGGALVSYLWKLCEAPYNKTLDWSKWYIFWSDERAVAKNHADSNYKLTKEGFLSKVPILSGHVYSINDNATVEDAATDYEFVIRQLVKVRTVGVSESNDCPKFDLILLGMGSDGHVASLFPNHQALELKDDWVTYITDSPQPPPERITFTLPVINSASNVAILATGDDKANAVHLAVSDSSNSQDAPTSLPARMVQPTDGKLVWFLDSASASLLEATTDDAAYEQHREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSS0</accession>
    <name>I1GSS0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100823439</name>
        <name evidence="7" type="ORF">BRADI_1g22710v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cystatin family. Phytocystatin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562768.1">
        <property type="nucleotide sequence ID" value="XM_003562720.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSS0"/>
    <dbReference type="STRING" id="15368.I1GSS0"/>
    <dbReference type="MEROPS" id="I25.056"/>
    <dbReference type="EnsemblPlants" id="KQK15428">
        <property type="protein sequence ID" value="KQK15428"/>
        <property type="gene ID" value="BRADI_1g22710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823439"/>
    <dbReference type="Gramene" id="KQK15428">
        <property type="protein sequence ID" value="KQK15428"/>
        <property type="gene ID" value="BRADI_1g22710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823439"/>
    <dbReference type="eggNOG" id="ENOG502S46Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113093_2_2_1"/>
    <dbReference type="OMA" id="FVYEQVW"/>
    <dbReference type="OrthoDB" id="2016588at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027214">
        <property type="entry name" value="Cystatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47116">
        <property type="entry name" value="PHLOEM FILAMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982590" description="Cystatin domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="6">
        <location>
            <begin position="31"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK15428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="123" mass="13355" checksum="62D56F4B0677E661" modified="2012-06-13" version="1">MARTLLLLAVLAAIAAASGSAAGIDDDLDRLIVGGWEPIRDVSEPHIQELGSWAVAQHARLATDRLRFLRVTRGEEQVVAGMNYCLFVDAQDAAGRSAPYVAVVYEQSWTNTRELTSFEPVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1GU20</accession>
    <name>I1GU20_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100826734</name>
        <name evidence="14" type="ORF">BRADI_1g26440v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752953.1">
        <property type="nucleotide sequence ID" value="XM_014897467.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GU20"/>
    <dbReference type="FunCoup" id="I1GU20">
        <property type="interactions" value="510"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GU20"/>
    <dbReference type="EnsemblPlants" id="KQK16045">
        <property type="protein sequence ID" value="KQK16045"/>
        <property type="gene ID" value="BRADI_1g26440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826734"/>
    <dbReference type="Gramene" id="KQK16045">
        <property type="protein sequence ID" value="KQK16045"/>
        <property type="gene ID" value="BRADI_1g26440v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826734"/>
    <dbReference type="eggNOG" id="KOG1002">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_2_10_1"/>
    <dbReference type="OMA" id="DHIMLRR"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="172"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="608"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="691"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="331"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK16045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="861" mass="96939" checksum="03BF40BB6FEA00D9" modified="2012-06-13" version="1">MPRRKGNASASGSRQRRRPDSDSDGDESVPSSDDTSDFEFVADLEEAADDADDFVSDEEDVAPAPAPAPALAVPRYVYVPPPPGQPARRRRAKARGRKKGKAADNRPPLPWEEWEEANEQWLDERIETADLEEADASHAPAALPAVPTAEPPPEVLLQLLRFQKEWLAWALAQEASVSRGGILADEMGMGKTIQGIALVLTARQLRHPGSGPSSPPSLSLGLPIQRVGCTLVICPVVAVIQWAQEIERHTAKGSARVLLYYGARRGSQKYDFDTYDFVVTTYSTIEADYRKHIMPLKIRCEYCDKLFYPNKMKVHLTYYCGPDALRTEKQAKQMSKKWADKKGKGKRSGSKRKISAQEEEEDNEELGESERQSRGRSPLHSVRWERIILDEAHFIKDRRCNTARAVFALESEYKWALSGTPLQNRVGELYSLIRFLQVFPYSNYFCKDCDCKILDTNMKKQCDCGHSSVRHFCWWNKFIARPILYGGPEGRRAMILLKEKVLKGIVLRRTKKGRAADLALPPKIVTLRRDSFDRNEMEFYEALYTQSCTQFDSYVDAGTLLNNYAHIFDLLTRLRQAVDHPYLVAFSKSAELREGYKNEGNQTMESQCGICHDMAEDVVVTSCDHVFCKTCLIDYSATLGNVSCPSCSKPLTVDLTTKSSKGKVPANLKGGKRSGILGRLQNLADFKTSTKIDALREEIRNMIEHDGSSKGIVFSQFTSFLDLIEFSLQKSGVKCVQLNGKMNMSEKGRAIDTFINDPDCRIFLMSLKAGGVALNLTVASHVFLMDPWWNPAVESQAQDRIHRIGQFKPIRSVRFVIKDTVEERILQLQDKKRLVFEGTVGDSPEAMSKLTEADLKFLFQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>I1GU57</accession>
    <name>I1GU57_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g26775v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GU57"/>
    <dbReference type="EnsemblPlants" id="KQK16106">
        <property type="protein sequence ID" value="KQK16106"/>
        <property type="gene ID" value="BRADI_1g26775v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16106">
        <property type="protein sequence ID" value="KQK16106"/>
        <property type="gene ID" value="BRADI_1g26775v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1362087_0_0_1"/>
    <dbReference type="InParanoid" id="I1GU57"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK16106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="201" mass="21352" checksum="C5295E5B5B564B17" modified="2012-06-13" version="1">MDIEGWRAGTETGTGTREDADDGIVVVRPCVEPRIILPLLPANRAAKSEGQRSKVSKGQKEKGKKEPQEGQRRANRLAEVDDVDLQKRALAGEDGGSGVDPVCYTVLDVGVPLGGDAHVPHVRTPHSGDAAVSNEYERPAGEADVSHLCAPLVAEAATSHVCVLLSGDASDVDEWVTLAGDAQDGRSSGVEAWRMRERARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWT5</accession>
    <accession>A0A0Q3H3M8</accession>
    <name>I1GWT5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyl transferase 64 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100829754</name>
        <name evidence="6" type="ORF">BRADI_1g34550v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 64 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17456.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757418.1">
        <property type="nucleotide sequence ID" value="XM_014901932.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWT5"/>
    <dbReference type="FunCoup" id="I1GWT5">
        <property type="interactions" value="613"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GWT5"/>
    <dbReference type="EnsemblPlants" id="KQK17456">
        <property type="protein sequence ID" value="KQK17456"/>
        <property type="gene ID" value="BRADI_1g34550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829754"/>
    <dbReference type="Gramene" id="KQK17456">
        <property type="protein sequence ID" value="KQK17456"/>
        <property type="gene ID" value="BRADI_1g34550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829754"/>
    <dbReference type="eggNOG" id="KOG1022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013906_1_1_1"/>
    <dbReference type="OMA" id="GGGTKEY"/>
    <dbReference type="OrthoDB" id="5954868at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GWT5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053318">
        <property type="entry name" value="GT64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015338">
        <property type="entry name" value="GT64_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48410">
        <property type="entry name" value="GLYCOSYLINOSITOL PHOSPHORYLCERAMIDE MANNOSYL TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48410:SF1">
        <property type="entry name" value="GLYCOSYLINOSITOL PHOSPHORYLCERAMIDE MANNOSYL TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09258">
        <property type="entry name" value="Glyco_transf_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 64" evidence="5">
        <location>
            <begin position="134"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK17456.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="386" mass="42754" checksum="4BB5CCA9CDFC764E" modified="2012-06-13" version="1">MYNSMYSVVRTIHNHHHLSSIWKGKQLGVLLRLSSTGTGARANQSSLSAPMLLGVAKLQLQATAAAAFRRNGTRRRPLLVPVPARHPSGASAGRFAACFVACLLALAAATVLALTLRRLDPDAASPRGGGGGYVVVVNTWKRYGLLRRAVAHYSGCAGVDAVHVVWSEPREPPEALRRSVLNCSSAAEVRFEVNREDSLNNRFRPIQGLRTDAVFSVDDDLIVPCATLRFAFSVWRSAPSAMVGFVPRMHWLADPRSNTEEFRYASWWSVWRTGTYSMILSKASFFHKKYLDLYTNHMLPSIRDYVTENRNCEDIAISFLVANVTGAPPIWVQGRIYEIGSSGISSLKGHDLQRSRCLNKFAAMYGHMPLVATTAKAVDSRTSWFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1GXA4</accession>
    <name>I1GXA4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="2">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100828171</name>
        <name evidence="13" type="ORF">BRADI_1g35940v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563623.1">
        <property type="nucleotide sequence ID" value="XM_003563575.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GXA4"/>
    <dbReference type="FunCoup" id="I1GXA4">
        <property type="interactions" value="1795"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GXA4"/>
    <dbReference type="EnsemblPlants" id="KQK17663">
        <property type="protein sequence ID" value="KQK17663"/>
        <property type="gene ID" value="BRADI_1g35940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828171"/>
    <dbReference type="Gramene" id="KQK17663">
        <property type="protein sequence ID" value="KQK17663"/>
        <property type="gene ID" value="BRADI_1g35940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828171"/>
    <dbReference type="eggNOG" id="KOG1448">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_7_0_1"/>
    <dbReference type="OMA" id="LLPEHKC"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000007">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_B">
        <property type="entry name" value="RibP_PPkinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037515">
        <property type="entry name" value="Rib-P_diPkinase_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002758">
        <property type="entry name" value="PRK02812.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF41">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="12">
        <location>
            <begin position="87"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK17663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="396" mass="42521" checksum="D7D8A456052AF9C5" modified="2012-06-13" version="1">MATTAAASPSSSSSLLLSRRHGQSSSASRAARFRAPRCVLGVEQLRVLDEGKRVGGGEPMAAAWTPKSPAQQARLTALPLEARDSRLKIFSGTANRPLALEIASYLGVDLGKILIKRFADGEIYVQLQESVRGCDVFLVQPTCSPVNENLMELFIMIDACRRASARSITVVIPYFGYARADRKAQGREAITAKLAANLLTEAGSDRVIVCDIHSAQALGYFDIPVDHIYGQPVILDYLASKTISKDLVVVSPDVGGVVRARAFAKKLSDAPLAIVDKRRQGHNLSEVMHLIGDVKGKVAIMVDDMIDTAGTITSGAALLKQEGAEAVYACCTHAVLSPPAIERLSGGIFEEVIVTNSILLPEDKCFPQLTVLSMANLVAETIWHVHRDGSVSSIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1H0D9</accession>
    <name>I1H0D9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100825370</name>
        <name evidence="6" type="ORF">BRADI_1g47440v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560998.1">
        <property type="nucleotide sequence ID" value="XM_003560950.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H0D9"/>
    <dbReference type="FunCoup" id="I1H0D9">
        <property type="interactions" value="1598"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H0D9"/>
    <dbReference type="EnsemblPlants" id="KQK19290">
        <property type="protein sequence ID" value="KQK19290"/>
        <property type="gene ID" value="BRADI_1g47440v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825370"/>
    <dbReference type="Gramene" id="KQK19290">
        <property type="protein sequence ID" value="KQK19290"/>
        <property type="gene ID" value="BRADI_1g47440v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825370"/>
    <dbReference type="eggNOG" id="KOG0324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069001_1_2_1"/>
    <dbReference type="OMA" id="HSGVQGM"/>
    <dbReference type="OrthoDB" id="412286at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF17">
        <property type="entry name" value="OS06G0182100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="5">
        <location>
            <begin position="44"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK19290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="254" mass="26697" checksum="F1D2B72CD9253591" modified="2012-06-13" version="1">MASSASSSSSSYSSAGSISTPRPPRHPHGAAAASSSSSSTSSSSPVFLNVYDVTPANGYARWLGLGVYHSGVQVHGVEYAYGAHEGNGSGIFEVLPRRCPGYAFRESVLVGTTELTRAQVRAVMAGELAAEFPGDAYNLVSRNCNHFCDAACRRLVAQARIPRWVNRLAKIGVVFTCVIPSGNGKQQQARRRNKGEPSARARSRSARQAADAAAPPRARTFFRSLSVGGGTKNVTPTPTPPLDPVLTSTSSVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1H186</accession>
    <name>I1H186_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">microtubule-severing ATPase</fullName>
            <ecNumber evidence="7">5.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100828523</name>
        <name evidence="12" type="ORF">BRADI_1g49940v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.</text>
            <dbReference type="EC" id="5.6.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557126.1">
        <property type="nucleotide sequence ID" value="XM_003557078.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H186"/>
    <dbReference type="STRING" id="15368.I1H186"/>
    <dbReference type="EnsemblPlants" id="KQK19706">
        <property type="protein sequence ID" value="KQK19706"/>
        <property type="gene ID" value="BRADI_1g49940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828523"/>
    <dbReference type="Gramene" id="KQK19706">
        <property type="protein sequence ID" value="KQK19706"/>
        <property type="gene ID" value="BRADI_1g49940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828523"/>
    <dbReference type="HOGENOM" id="CLU_000688_21_12_1"/>
    <dbReference type="OrthoDB" id="10251136at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H186">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008568">
        <property type="term" value="F:microtubule severing ATPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02679">
        <property type="entry name" value="MIT_spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19524">
        <property type="entry name" value="RecA-like_spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000066">
        <property type="entry name" value="AAA-type ATPase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000019">
        <property type="entry name" value="AAA-type ATPase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000958">
        <property type="entry name" value="Spastin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
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        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
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        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF86">
        <property type="entry name" value="SPASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MIT"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="11">
        <location>
            <begin position="45"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="245"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="126"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="150"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00038871"/>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SMART" id="SM00382"/>
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        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="KQK19706.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK19706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="485" mass="53685" checksum="BAA60CB8D63904B4" modified="2012-06-13" version="1">MSFLRALADSLSSLFAPSPEAPMADAAPPPPSAAAVVGERVAVKLRGYFELAKEEIDKAVRAEEWGLPEDADAHYRNALRVMLEAKAARVPDAVSSSERGQVRMYQEKIAKWQTQVEERLRVLGRRSGAAAPVPKKVATNNHLNRPERPASTSFSKSTLQSRPTFNRGGQASSHQNSNGGSKPMQKAGGKDDDKLVEMINTTIVDRSPSVKWDDVAGLDKAKQALMEMVILPTKRRDLFTGLRRPAKGLLLFGPPGNGKTMLAKAVASESEATFFNVSASSLTSKWVGEAEKLVRTLFMVAIDRQPSVIFMDEIDSVMSTRLANENDASRRLKSEFLIQFDGVTSNPDDLVIVIGATNKPQELDDAVLRRLVKRIYVPLPDPNVRRLLLKNQLKGQAFKLSNHDFERLAVETEGYSGSDLRALCEEAAMMPIRELGPQNILTIKANQLRPLKYEDFKNAMTAIRPSLQKSKWDELEKWNDEFGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1H333</accession>
    <name>I1H333_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100833729</name>
        <name evidence="3" type="ORF">BRADI_1g55460v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557480.1">
        <property type="nucleotide sequence ID" value="XM_003557432.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H333"/>
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    </dbReference>
    <dbReference type="STRING" id="15368.I1H333"/>
    <dbReference type="EnsemblPlants" id="KQK20581">
        <property type="protein sequence ID" value="KQK20581"/>
        <property type="gene ID" value="BRADI_1g55460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833729"/>
    <dbReference type="Gramene" id="KQK20581">
        <property type="protein sequence ID" value="KQK20581"/>
        <property type="gene ID" value="BRADI_1g55460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833729"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35700">
        <property type="entry name" value="OS07G0181800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OS07G0181800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK20581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="76" mass="8526" checksum="02577347EFB77A48" modified="2012-06-13" version="1">MSSLGTSKGILEIAKFGVYLAVPITLTYLVATDSQSIKKLMGLRPYVVYPPEGPRPPPPEELRERAREIARSRRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1H355</accession>
    <name>I1H355_BRADI</name>
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        <recommendedName>
            <fullName evidence="13">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100835800</name>
        <name evidence="14" type="ORF">BRADI_1g55630v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228292.1">
        <property type="nucleotide sequence ID" value="XM_010229990.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228293.1">
        <property type="nucleotide sequence ID" value="XM_010229991.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313196.1">
        <property type="nucleotide sequence ID" value="XM_024457428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H355"/>
    <dbReference type="STRING" id="15368.I1H355"/>
    <dbReference type="EnsemblPlants" id="KQK20612">
        <property type="protein sequence ID" value="KQK20612"/>
        <property type="gene ID" value="BRADI_1g55630v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK20613">
        <property type="protein sequence ID" value="KQK20613"/>
        <property type="gene ID" value="BRADI_1g55630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835800"/>
    <dbReference type="Gramene" id="KQK20612">
        <property type="protein sequence ID" value="KQK20612"/>
        <property type="gene ID" value="BRADI_1g55630v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20613">
        <property type="protein sequence ID" value="KQK20613"/>
        <property type="gene ID" value="BRADI_1g55630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835800"/>
    <dbReference type="eggNOG" id="KOG0627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030308_1_0_1"/>
    <dbReference type="OMA" id="VIMEDMN"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000057">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF402">
        <property type="entry name" value="HEAT STRESS TRANSCRIPTION FACTOR A-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="13">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="10"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="246"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="173"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK20612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="457" mass="50304" checksum="2BD8CC4F738B7F28" modified="2012-06-13" version="1">MDPLLSLVKEEQEGHGGGGGSPPAAVAAEDGPSTVAAAAVPRPMEGLHDVGPPPFLTKTYDMVDDQNSNHVVSWSPTNNSFVVWDPHAFATSLLPRHFKHSNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQRHLLKNIRRRKPPAHPASNQQSFGSSYLEVGHFGNDAEIDRLKRDKELLMAQVVKLRQEQQDTKARLKAMEDRLHGNEQKQQQMVTFLARVLRNPEFLKQLIAKNEMRKQLHDTISKKRRRRIDQGTEADHMGASSSLQQESPILFDPHVSMELLADGSVQLLADDIPPDLEGSGGFVADGIPPDMESSVEHVADGILPGLEGSVELLADGIPPDLEGSLDLLADGIPLNLEGSMELLADGIPSGLEGPGIKTNGVMEPHGFDLGICGMQQNRAQGVLKDNFWDDLLNEELGDEDDNPVIMEDMNLLSEKMGYLVSDTPTPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4G2</accession>
    <name>I1H4G2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 23">Peroxidase</fullName>
            <ecNumber evidence="5 23">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC100835072</name>
        <name evidence="25" type="ORF">BRADI_1g59537v3</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 23">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20 23">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="20 23">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20 23">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="20 23">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 23">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="5 23"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561522.1">
        <property type="nucleotide sequence ID" value="XM_003561474.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H4G2"/>
    <dbReference type="STRING" id="15368.I1H4G2"/>
    <dbReference type="EnsemblPlants" id="KQK21232">
        <property type="protein sequence ID" value="KQK21232"/>
        <property type="gene ID" value="BRADI_1g59537v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835072"/>
    <dbReference type="Gramene" id="KQK21232">
        <property type="protein sequence ID" value="KQK21232"/>
        <property type="gene ID" value="BRADI_1g59537v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835072"/>
    <dbReference type="eggNOG" id="ENOG502QQVF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010543_0_3_1"/>
    <dbReference type="OMA" id="HCSSYAD"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000008">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20 23" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 23" id="KW-0349">Heme</keyword>
    <keyword evidence="17 23" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="13 20 23" id="KW-0408">Iron</keyword>
    <keyword evidence="9 20 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 23" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 23" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="16" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 23" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982692" description="Peroxidase" evidence="23">
        <location>
            <begin position="20"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="24">
        <location>
            <begin position="26"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="21">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="36"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="69"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="122"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="200"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KQK21232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="330" mass="34688" checksum="CFC3DBBCC75C3259" modified="2012-06-13" version="1">MAAAWLAALAFALAPLLCASEGNGTGIAFGYYNATCPEAETIVFRETARILRASPDLAASLLRLHYHDCFVQGCDASVLLDTTDAANPTEKDGKPNESLRGFDAVARVKDKLETACPATVSCADLLALMARDAVLLSKGPRWAVALGRRDGRSSSAGNCGELPPLYGNMTVMVEVFAGKGMDAKDIAVLSGAHTLGKAHCSSFADRLYSGANATCVTDPALDGRYAARLRLRCPGNNGGNGGAAAEMDAGSCGTFDTSYYRHVASKRGLLQSDAALMEHPVTAAYVRRAATGRYDGHFFSDFAESMAKMGAVGVLTGDQGEIRIKCNRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1H550</accession>
    <name>I1H550_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glabrous enhancer-binding protein-like DBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100841096</name>
        <name evidence="4" type="ORF">BRADI_1g61477v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GeBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228580.1">
        <property type="nucleotide sequence ID" value="XM_010230278.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H550"/>
    <dbReference type="STRING" id="15368.I1H550"/>
    <dbReference type="EnsemblPlants" id="KQK21550">
        <property type="protein sequence ID" value="KQK21550"/>
        <property type="gene ID" value="BRADI_1g61477v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841096"/>
    <dbReference type="Gramene" id="KQK21550">
        <property type="protein sequence ID" value="KQK21550"/>
        <property type="gene ID" value="BRADI_1g61477v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841096"/>
    <dbReference type="eggNOG" id="ENOG502QUAW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057404_0_0_1"/>
    <dbReference type="OMA" id="MNAMPLC"/>
    <dbReference type="OrthoDB" id="669440at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007592">
        <property type="entry name" value="GEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053932">
        <property type="entry name" value="GeBP-like_DBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31662">
        <property type="entry name" value="BNAANNG10740D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31662:SF2">
        <property type="entry name" value="OS03G0370250 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04504">
        <property type="entry name" value="GeBP-like_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glabrous enhancer-binding protein-like DBD" evidence="3">
        <location>
            <begin position="69"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK21550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="354" mass="38604" checksum="A6DB5144ECFAF517" modified="2012-06-13" version="1">MLPMVDDPSAAAGAAASSSFPDADAYGNGDSEDIDFPVDPIPNPPFSSSATVPAPASAAAAVGERRPLFQRLWTEEDEIVILRAFAEFTAQRGTAFASHQYDTEPFYEEMRRRLQLDFSKSQLAEKLRRLKRKYRNCVERLRGSGASFTFRSPHEQAVFEIARNIWRPSSDKHGRDPSAGDSDDDATVTATNAGANGDAKSPSSSRAQRRVRRRRTADCTADATEVPQPYTPSTMPVKTEESLPAFFPQVSMDGAEHVVPPAAQPAANTESSVLTPLFKEMVRAMLGMGGCPSPLGLGAKVPEPPSAILGIPMEGEKWRQQRILELEVYLRRIDLLQDEVKSALEELKATPAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1H574</accession>
    <name>I1H574_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100844144</name>
        <name evidence="13" type="ORF">BRADI_1g61690v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228588.1">
        <property type="nucleotide sequence ID" value="XM_010230286.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H574"/>
    <dbReference type="FunCoup" id="I1H574">
        <property type="interactions" value="619"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK21579">
        <property type="protein sequence ID" value="KQK21579"/>
        <property type="gene ID" value="BRADI_1g61690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844144"/>
    <dbReference type="Gramene" id="KQK21579">
        <property type="protein sequence ID" value="KQK21579"/>
        <property type="gene ID" value="BRADI_1g61690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844144"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_21_2_1"/>
    <dbReference type="OMA" id="KPFTANC"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000228">
        <property type="entry name" value="Serine/threonine-protein kinase BIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="102"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK21579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="447" mass="48376" checksum="3F46E58242BFAE1F" modified="2012-06-13" version="1">MPRPWRPVLASATKCCAAEDAVVTAGAGQGLARCRPARSEFSRRLASFRRLSSMANSPATPMDGKDVDDDDDADGEAGQMGVGALQLHSFSLSELRGVTHDFSSGYLLGEGGFGTVHKGFVDAGMRPGLEPQPVAVKQLDIAGHQGHREWMAEVIFLGQFRHQHLVKLLGYCCEDEERLLVYEFMPRGSLENHLFKRISATLPWGTRLKVAIGAAKGLAFLHGAHKPVIYRDFKASNILLDSEFTAKLSDFGLAKMGPEGEDTHVTTRVMGTHGYAAPEYVQTGHLTMKSDVYSFGVVLLELLTGRRAMENVRGRTAHAEQTIKLVEWTRPYLASSRRLRCIMDPRLAGHYSVKGARAVAHLACECTALQPRDRPRMAAVVESLERLQGFKDMAVSVGLWPTAPVVAGRNALSAKFRAEMKGAGAGAGAGVGVGAVSRRRSASAKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1H5B3</accession>
    <name>I1H5B3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100835760</name>
        <name evidence="11" type="ORF">BRADI_1g62000v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the villin/gelsolin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228602.1">
        <property type="nucleotide sequence ID" value="XM_010230300.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753026.1">
        <property type="nucleotide sequence ID" value="XM_014897540.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311703.1">
        <property type="nucleotide sequence ID" value="XM_024455935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H5B3"/>
    <dbReference type="STRING" id="15368.I1H5B3"/>
    <dbReference type="EnsemblPlants" id="PNT77391">
        <property type="protein sequence ID" value="PNT77391"/>
        <property type="gene ID" value="BRADI_1g62000v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77392">
        <property type="protein sequence ID" value="PNT77392"/>
        <property type="gene ID" value="BRADI_1g62000v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77393">
        <property type="protein sequence ID" value="PNT77393"/>
        <property type="gene ID" value="BRADI_1g62000v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77394">
        <property type="protein sequence ID" value="PNT77394"/>
        <property type="gene ID" value="BRADI_1g62000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835760"/>
    <dbReference type="Gramene" id="PNT77391">
        <property type="protein sequence ID" value="PNT77391"/>
        <property type="gene ID" value="BRADI_1g62000v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77392">
        <property type="protein sequence ID" value="PNT77392"/>
        <property type="gene ID" value="BRADI_1g62000v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77393">
        <property type="protein sequence ID" value="PNT77393"/>
        <property type="gene ID" value="BRADI_1g62000v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77394">
        <property type="protein sequence ID" value="PNT77394"/>
        <property type="gene ID" value="BRADI_1g62000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835760"/>
    <dbReference type="eggNOG" id="KOG0443">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002568_2_0_1"/>
    <dbReference type="OMA" id="DPNIWSA"/>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11293">
        <property type="entry name" value="gelsolin_S4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11291">
        <property type="entry name" value="gelsolin_S6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000001">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000002">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.950.10:FF:000006">
        <property type="entry name" value="Villin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000039">
        <property type="entry name" value="Villin-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000028">
        <property type="entry name" value="Villin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000038">
        <property type="entry name" value="Villin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF98">
        <property type="entry name" value="VILLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0117">Actin capping</keyword>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HP" evidence="10">
        <location>
            <begin position="890"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="770"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="771"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="796"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="888"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT77392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="955" mass="105686" checksum="F12896B07984D7FF" modified="2012-06-13" version="1">MSTAKQVLDPAFQGAGQKPGTEIWRIEDFKPVPLPKSDYGKFYCGDSYIILQTTCNRGGAYLFDIHFWIGKDSSQDEAGTSAIKTVELDTMLGGRAVQHREPQGYESDKFLSYFKPCIIPLEGGFASGFKKPEEDKFETRLYICKGKRAIRVKEVPFARSALNHDDVFILDSEKKIYQFNGANSNIQERAKALEVIQHLKEKYHEGVCDVAIVDDGKLQAESDSGEFWVLFGGFAPIGKKAVSDDDVILETAPTKLYSINNGKLKLEDIVLTKSILENTKCFLLDCGSELFVWVGRVTQVDDRKAASVAVEEFIVNQNRPKTTRVTQVIQGYETHTFKSKFESWPISSTAGNASMEDGRGKVAALLKKKGDVKGASKTSATVNEEVPPLLEGGGKLEVWYIDGSAKTALPKEDLGKFYSGDCYIVLYTYHSGDKREEFYLTYWIGKDSVLDDQQMACQMANTIWNAMKGRPVLGRIYQGKEPPQFIALFQPMVIFKGGISFRYKKFIEEKGLKDATYSATGIALVQIIGTSTHNNKTLQVDAVSTSLSSTDCFVLQSGNSMFTWIGNTSSHEQQQWAAKVAEFLKPGGTIKHCKEGTESSAFWSALGGKQNYTNRNASQDVLREPHLYTFSFRNGKLEVTEVFNFSQDDLLTEDVMVLDTHAEVFVWMGQCVDTKEKQNAFEIGQKYVEHAVTFEGLSPDVPLYKFSEGNEPCFFRTYFSWDNTRAVIHGNSFQKKLSLLFGMRSESGSKGSGDGGPTQRASALAALSSAFNPSSQDKQSNDGPRSSGDGGPTQRASALAALSSALNPSSKPKSPQSQSRPGQGSQRAAAVAALSNVLTAESPRNDAEKTELAPSDASPLGEAAGSSEFDEGPGERTEPDVSQEETANENGGQTTFSYERLISKSTDPVRGIDYKRRETYLSDDEFQTVFSMTKDEFYQQPRWKQELQKRKADLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1H867</accession>
    <name>I1H867_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100837114</name>
        <name evidence="9" type="ORF">BRADI_1g70220v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558507.1">
        <property type="nucleotide sequence ID" value="XM_003558459.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H867"/>
    <dbReference type="STRING" id="15368.I1H867"/>
    <dbReference type="EnsemblPlants" id="KQK22939">
        <property type="protein sequence ID" value="KQK22939"/>
        <property type="gene ID" value="BRADI_1g70220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837114"/>
    <dbReference type="Gramene" id="KQK22939">
        <property type="protein sequence ID" value="KQK22939"/>
        <property type="gene ID" value="BRADI_1g70220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837114"/>
    <dbReference type="eggNOG" id="KOG3065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061058_0_0_1"/>
    <dbReference type="OMA" id="TANHMEQ"/>
    <dbReference type="OrthoDB" id="19261at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H867">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15861">
        <property type="entry name" value="SNARE_SNAP25N_23N_29N_SEC9N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000031">
        <property type="entry name" value="SNAP25 homologous protein SNAP33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000040">
        <property type="entry name" value="SNAP25 homologous protein SNAP33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044766">
        <property type="entry name" value="NPSN/SNAP25-like_N_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF29">
        <property type="entry name" value="OS03G0212400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="8">
        <location>
            <begin position="184"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK22939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="249" mass="27588" checksum="AFA1893B8CDAF2E3" modified="2012-06-13" version="1">MPVSMDKKQNSLGTPVLRTNPFDSDSDTDSEVLSRPSRAQSAPANCAKSVQELEDNAVRRAEETSCKVNDCVRAAEAIREDATQILMTLHQQGEQITQTHRVAVDIEQDLSVSERLLGSLGGLFSKTWRPKKNQQIKGPISQSNSFKSTSYHMEERQKLGIASTPPQSHNPVRHSPATALEKVQAEKAKQDDAFSDLSNSLGQLKLMAMDMGTEIERQNKSLDTLSDDVDELNFRLKGANQRGRRLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1H8J9</accession>
    <name>I1H8J9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">serine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100833118</name>
        <name evidence="17" type="ORF">BRADI_1g71300v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Ser) + L-serine + ATP = L-seryl-tRNA(Ser) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558579.1">
        <property type="nucleotide sequence ID" value="XM_003558531.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H8J9"/>
    <dbReference type="FunCoup" id="I1H8J9">
        <property type="interactions" value="2732"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H8J9"/>
    <dbReference type="EnsemblPlants" id="KQK23100">
        <property type="protein sequence ID" value="KQK23100"/>
        <property type="gene ID" value="BRADI_1g71300v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK23101">
        <property type="protein sequence ID" value="KQK23101"/>
        <property type="gene ID" value="BRADI_1g71300v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK23102">
        <property type="protein sequence ID" value="KQK23102"/>
        <property type="gene ID" value="BRADI_1g71300v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833118"/>
    <dbReference type="Gramene" id="KQK23100">
        <property type="protein sequence ID" value="KQK23100"/>
        <property type="gene ID" value="BRADI_1g71300v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK23101">
        <property type="protein sequence ID" value="KQK23101"/>
        <property type="gene ID" value="BRADI_1g71300v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK23102">
        <property type="protein sequence ID" value="KQK23102"/>
        <property type="gene ID" value="BRADI_1g71300v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833118"/>
    <dbReference type="eggNOG" id="KOG2509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_0_1_1"/>
    <dbReference type="OMA" id="GYTPCFR"/>
    <dbReference type="OrthoDB" id="593770at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.40:FF:000003">
        <property type="entry name" value="Serine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000026">
        <property type="entry name" value="Seryl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="16">
        <location>
            <begin position="191"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="49"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="266"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="282"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="355"/>
            <end position="358"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="11">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK23101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="456" mass="51421" checksum="EFA8FF08A4B2E159" modified="2012-06-13" version="1">MLDINLFRKEKGGDPDLVRNSQRSRFASVELVDEVIVLDEEWRQRQFELDKIRQELNKTSKEIGKLKAKRLDATVLIQSTEEIKSRLAAKETEVQDAKSTLDAKLVTIGNLVHESVPVSNDEANNAIVRTWGERRLEGNLKNHVDLCIMLDIVALEKGADVAGGRGYFLKGNGVLLNMALINFGLAFLGIRGYTQMQTPFFMRKETMARCAQLAQFDEELYKVTGDGEEKYLIATSEQPLCAYHLGDRIYPADLPIRYAGYSTCFRKEAGSHGRDTAGIFRVHQFEKIEQFCVTSPNDNNSWELHEEMIKNSEDFYKEIGLPYQLVSIVSGALNDAAAKKYDLEAWFPASKTYRELVSCSNCTDYQARRLGIGFGQKKNDEQSKQFVHMLNSTLTATERTLCCILENFQKEDGVEVPKALRPFMGGIDFLPFKKPLDSKQAADSKPNKSKPKGNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCC3</accession>
    <name>I1HCC3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104582497</name>
        <name evidence="3" type="ORF">BRADI_2g04140v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230493.1">
        <property type="nucleotide sequence ID" value="XM_010232191.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCC3"/>
    <dbReference type="FunCoup" id="I1HCC3">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK02857">
        <property type="protein sequence ID" value="KQK02857"/>
        <property type="gene ID" value="BRADI_2g04140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104582497"/>
    <dbReference type="Gramene" id="KQK02857">
        <property type="protein sequence ID" value="KQK02857"/>
        <property type="gene ID" value="BRADI_2g04140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104582497"/>
    <dbReference type="eggNOG" id="ENOG502SDM5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067213_1_0_1"/>
    <dbReference type="OMA" id="RVTSIWG"/>
    <dbReference type="OrthoDB" id="679127at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK02857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="231" mass="25766" checksum="542B0331DD643C83" modified="2012-06-13" version="1">MASPRPATWSVSACLRYRGGLEIRAAAENVVLPWWGHGGERLSFLLRLRRRLRLAVTSQCARAPAPASADPESKEKKKKPRALKLLRFLRSRLARAPSALRRKKPPPPRAEDEDDRGDPRAQPVPCFLTRALMRPAKTKTTATVLCFVAALAVAVVALRLVAGFVIPPPTVSCTSWRCFLAKKLAKKLLGPPLQEWFSRIGLRILEFLDPPAALRDWPGLPKLGLKWLFNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCH3</accession>
    <name>I1HCH3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100843564</name>
        <name evidence="3" type="ORF">BRADI_2g04560v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569410.1">
        <property type="nucleotide sequence ID" value="XM_003569362.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCH3"/>
    <dbReference type="FunCoup" id="I1HCH3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK02939">
        <property type="protein sequence ID" value="KQK02939"/>
        <property type="gene ID" value="BRADI_2g04560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843564"/>
    <dbReference type="Gramene" id="KQK02939">
        <property type="protein sequence ID" value="KQK02939"/>
        <property type="gene ID" value="BRADI_2g04560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843564"/>
    <dbReference type="eggNOG" id="ENOG502RZC2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122141_1_0_1"/>
    <dbReference type="OMA" id="YVDYTKH"/>
    <dbReference type="OrthoDB" id="1699369at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.20:FF:000005">
        <property type="entry name" value="Uncharacterized protein At2g34160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014560">
        <property type="entry name" value="UCP030333_Alba"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947">
        <property type="entry name" value="DNA/RNA-BINDING PROTEIN ALBA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947:SF14">
        <property type="entry name" value="OS01G0173100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030333">
        <property type="entry name" value="UCP030333_Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="2">
        <location>
            <begin position="41"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK02939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="150" mass="16257" checksum="D5BAF7E02EDEFBB1" modified="2012-06-13" version="1">MAVEEITEGVRALAVQDGEAAAPPGGAAEGQQRRGTNSSSNRIQVSNTKKPLFFYVNLAKRYMQQHGDVELSALGMAIATVVTVAEILKNNGLAVEKKIRTSTVEINDESRGRPFQKAKIEIELGKSEKFDELMAAAAEDREAEEGEEHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1HG59</accession>
    <name>I1HG59_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100831946</name>
        <name evidence="1" type="ORF">BRADI_2g15710v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567844.1">
        <property type="nucleotide sequence ID" value="XM_003567796.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HG59"/>
    <dbReference type="FunCoup" id="I1HG59">
        <property type="interactions" value="1290"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HG59"/>
    <dbReference type="EnsemblPlants" id="KQK04749">
        <property type="protein sequence ID" value="KQK04749"/>
        <property type="gene ID" value="BRADI_2g15710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831946"/>
    <dbReference type="Gramene" id="KQK04749">
        <property type="protein sequence ID" value="KQK04749"/>
        <property type="gene ID" value="BRADI_2g15710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831946"/>
    <dbReference type="eggNOG" id="ENOG502QPII">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112670_0_0_1"/>
    <dbReference type="OMA" id="NDHPRDD"/>
    <dbReference type="OrthoDB" id="10266005at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HG59">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35716:SF1">
        <property type="entry name" value="OS05G0574700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35716">
        <property type="entry name" value="OS05G0574700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK04749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="211" mass="24050" checksum="3CBE594ED9FA1074" modified="2012-06-13" version="1">MASLAPATPAKPIGLLPCPIRGGGIGSARPGLATAAPRLRVSASASASDVPDFLSSDWLETRKKKPLGPRLNYNAEEAVEYQLEALKYNDRPRQDYGIEVMYRFAGFDPFERSTYFGRQFDLGQFERFRRIFHHSTYRVLLAHKERKILSSLWVEENQYKQRVWVRGARPEEEEIFQFTMIQRVGGSWDGYWLTESLTNDDGDTFSGGVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1HHE2</accession>
    <name>I1HHE2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100821198</name>
        <name evidence="7" type="ORF">BRADI_2g19270v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568066.1">
        <property type="nucleotide sequence ID" value="XM_003568018.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HHE2"/>
    <dbReference type="EnsemblPlants" id="KQK05294">
        <property type="protein sequence ID" value="KQK05294"/>
        <property type="gene ID" value="BRADI_2g19270v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821198"/>
    <dbReference type="Gramene" id="KQK05294">
        <property type="protein sequence ID" value="KQK05294"/>
        <property type="gene ID" value="BRADI_2g19270v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821198"/>
    <dbReference type="eggNOG" id="KOG1595">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044407_1_0_1"/>
    <dbReference type="OMA" id="SACSWAD"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045234">
        <property type="entry name" value="Unkempt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057444">
        <property type="entry name" value="Znf-CCCH_AtC3H23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493">
        <property type="entry name" value="UNKEMPT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493:SF44">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25512">
        <property type="entry name" value="zf-CCCH_AtC3H23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="109"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="109"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK05294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="276" mass="29775" checksum="541FAB4AAA8157A5" modified="2012-06-13" version="1">MVSHEQLLQLDPTALAFSWAAEPAPSVSEIPPQLLVALGEYLSSGAAAGHNEGDAGAVADAADAEADEEFMMYEFKVRRCARARSHDWTACPYAHPGEAARRRDPRRVAYAGEPCPDFRRRPGAACPRGNSCPLAHGTFELWLHPSRYRTRPCRAGTACRRRVCFFAHTPAELRAAAGHKAGGDISPLAALSPKSTLTSLWESPPVSPVEGGRGWWVDAAAGDEGDADAEVEELMLAMRELNFRQAAAKAASRTQVLPPVTEDDGPDFGWVSELVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1HHF2</accession>
    <name>I1HHF2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="12">ATP-PFK</shortName>
            <shortName evidence="12">Phosphofructokinase</shortName>
            <ecNumber evidence="12">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100821809</name>
        <name evidence="12" type="synonym">PFK</name>
        <name evidence="15" type="ORF">BRADI_2g19326v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Allosterically activated by AMP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="12"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568068.1">
        <property type="nucleotide sequence ID" value="XM_003568020.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HHF2"/>
    <dbReference type="STRING" id="15368.I1HHF2"/>
    <dbReference type="EnsemblPlants" id="KQK05302">
        <property type="protein sequence ID" value="KQK05302"/>
        <property type="gene ID" value="BRADI_2g19326v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821809"/>
    <dbReference type="Gramene" id="KQK05302">
        <property type="protein sequence ID" value="KQK05302"/>
        <property type="gene ID" value="BRADI_2g19326v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821809"/>
    <dbReference type="eggNOG" id="KOG2440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="ETNRMAN"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HHF2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000002">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01981">
        <property type="entry name" value="Phosphofructokinase_II_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050929">
        <property type="entry name" value="PFKA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012004">
        <property type="entry name" value="PyroP-dep_PFK_TP0108"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005301">
        <property type="entry name" value="PRK06830.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45770">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="14">
        <location>
            <begin position="152"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="511"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="512"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="222"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="247"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="276"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="321"/>
            <end position="323"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="433"/>
            <end position="436"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity; cannot use PPi as phosphoryl donor" evidence="12">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK05302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="553" mass="60818" checksum="76ECD9C995FE1A81" modified="2012-06-13" version="1">MASHIILPPDDEEQRQRDEEEDGGQSASEHAPLPFSAMCVRISRDSYPNLRALRNASAVSLADAAYAKISEGDFGYVLDDVPHLADYLPDIPTYPNPLQDHPAYSTVKQYFVNEDDTVPQKVVVQKNSRRGVHFRRAGPRQRVYFESDEVKACIVTCGGLCPGLNTVIRELVCGLSHMYNVNKIYGIQNGYKGFYSSNYLAVTPKTVNDIHKRGGTVLGTSRGGHDTKKIVDNIQDRGINQVYIIGGDGTQKGAYEIYKEIRKRGLKVAVAGVPKTIDNDIAIIDKSFGFDSAVEEAQRAIDSAHVEACSAENGIGLVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYMDGEGGLLQYIEKRLKENKHMVIVVAEGAGQDIIAKSIPSSDQQDASGNKLLLDIGLWLTHKIKDHFKSKKVEMTIKYIDPTYMIRAIPSNASDNVYCTLLAHSAIHGAMAGYSFTVGMVNGRHTYIPFYRVTSTRNKVKITDRMWARLLSSTNQPSFLSQKDIDEAREADRLANKPPLPSGANQSVANAFGQSASSSSNGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HHT0</accession>
    <name>I1HHT0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100843351</name>
        <name evidence="10" type="ORF">BRADI_2g20280v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568142.1">
        <property type="nucleotide sequence ID" value="XM_003568094.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HHT0"/>
    <dbReference type="STRING" id="15368.I1HHT0"/>
    <dbReference type="MEROPS" id="C19.A13"/>
    <dbReference type="EnsemblPlants" id="KQK05481">
        <property type="protein sequence ID" value="KQK05481"/>
        <property type="gene ID" value="BRADI_2g20280v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK05482">
        <property type="protein sequence ID" value="KQK05482"/>
        <property type="gene ID" value="BRADI_2g20280v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843351"/>
    <dbReference type="Gramene" id="KQK05481">
        <property type="protein sequence ID" value="KQK05481"/>
        <property type="gene ID" value="BRADI_2g20280v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05482">
        <property type="protein sequence ID" value="KQK05482"/>
        <property type="gene ID" value="BRADI_2g20280v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843351"/>
    <dbReference type="eggNOG" id="KOG1865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029502_0_0_1"/>
    <dbReference type="OMA" id="CCNDSHI"/>
    <dbReference type="OrthoDB" id="420187at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02661">
        <property type="entry name" value="Peptidase_C19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000118">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF758">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="22"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="418"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK05482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="641" mass="70730" checksum="0FE3F2589888F3E9" modified="2012-06-13" version="1">MAPTAQPPSPPPPRRPRLGPPLGLKNLGNTCYLNSVLQCLASTPPLANFCLNSRHSNLCQKVFPNRDKECAFCVLERQIVRMLRAEAGALDSPGKIIRCLPLFAEHFRWGRQEDAHEFLRYVIDACHTACLRVRKRLPAASANGDCGQEEGRGQGNCMLMRETFGGALLSQVKCLVCKGESNKTDEIMDISLDLLGSSSVGDALACFFRPELLEGANKYSCERCKKLTSARKQMFILRAPKVLVIQLKRFEGINGGKINRNIGFKESLILSDFMYNKNQDSQPVYNLFASIVHSGFSPESGHYYAYVKDSIGRWYCCNDSHVSLSSSQDVLSEKVYILFYILSSKTQKHCTNGYSSSAVKSSNTNGNGISSATCSEPLKIQLVKQNGPCSIKGIAPLPLKNGKIAPGAHVKPIHLKNTGTEKVSSNGKQDLNLRNKPEVNGNVTLPESNGCEPGKSNGIISCDKMDVDSQRMLQDADGNGHPIHSADLQETTIDKATCAEKYPEQPSNDAASTLDKNIRSKENSKGSVLHHDEVPVNSVKEVVASAKDSALKHHLGEGKFKEMLAESASSELRSCGWVDDVYQFMRSTKRRCIENTGIAQDNDEIRKQLITDSGRVFKSKVPEALRERLIKTLKSYYEDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1HI54</accession>
    <name>I1HI54_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100836144</name>
        <name evidence="17" type="ORF">BRADI_2g21340v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568202.1">
        <property type="nucleotide sequence ID" value="XM_003568154.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HI54"/>
    <dbReference type="STRING" id="15368.I1HI54"/>
    <dbReference type="EnsemblPlants" id="KQK05638">
        <property type="protein sequence ID" value="KQK05638"/>
        <property type="gene ID" value="BRADI_2g21340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836144"/>
    <dbReference type="Gramene" id="KQK05638">
        <property type="protein sequence ID" value="KQK05638"/>
        <property type="gene ID" value="BRADI_2g21340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836144"/>
    <dbReference type="eggNOG" id="KOG1375">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_1_1_1"/>
    <dbReference type="OMA" id="VWIISAM"/>
    <dbReference type="OrthoDB" id="732292at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000005">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="429"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK05638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="447" mass="50336" checksum="03856E1AA3D3FA91" modified="2012-06-13" version="1">MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRSLTVPELTQQMWDSKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIAPRGLSMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVAEYQQYQDATAEDEEDYEEDEDLQAEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HKM0</accession>
    <name>I1HKM0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein phosphatase inhibitor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100828389</name>
        <name evidence="2" type="ORF">BRADI_2g31120v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568683.1">
        <property type="nucleotide sequence ID" value="XM_003568635.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HKM0"/>
    <dbReference type="STRING" id="15368.I1HKM0"/>
    <dbReference type="EnsemblPlants" id="KQK06893">
        <property type="protein sequence ID" value="KQK06893"/>
        <property type="gene ID" value="BRADI_2g31120v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK06894">
        <property type="protein sequence ID" value="KQK06894"/>
        <property type="gene ID" value="BRADI_2g31120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828389"/>
    <dbReference type="Gramene" id="KQK06893">
        <property type="protein sequence ID" value="KQK06893"/>
        <property type="gene ID" value="BRADI_2g31120v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06894">
        <property type="protein sequence ID" value="KQK06894"/>
        <property type="gene ID" value="BRADI_2g31120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828389"/>
    <dbReference type="eggNOG" id="ENOG502RYGC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="KKPAGCD"/>
    <dbReference type="OrthoDB" id="551302at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HKM0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="18861" checksum="5BABF5E69921F27B" modified="2012-06-13" version="1">MSSHRVKWNEDNLYEIESNKPVRQKITEPKTPYHPMIDEDGSLSPTRPFDKCLDETVNAEAILTALNGVASSSKSDAKDDGWASSDDDADAMEHEDGDSEVDKGAVSFKEHRRVHYDEFRKVKELMRTGSLTEEEPRSDDRGANNSQNKTTGEKAADSKSSPSPQTSSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1HKX7</accession>
    <name>I1HKX7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100821499</name>
        <name evidence="12" type="ORF">BRADI_2g32600v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568748.1">
        <property type="nucleotide sequence ID" value="XM_003568700.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HKX7"/>
    <dbReference type="FunCoup" id="I1HKX7">
        <property type="interactions" value="1667"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HKX7"/>
    <dbReference type="EnsemblPlants" id="KQK07056">
        <property type="protein sequence ID" value="KQK07056"/>
        <property type="gene ID" value="BRADI_2g32600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821499"/>
    <dbReference type="Gramene" id="KQK07056">
        <property type="protein sequence ID" value="KQK07056"/>
        <property type="gene ID" value="BRADI_2g32600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821499"/>
    <dbReference type="OrthoDB" id="756301at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HKX7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000009">
        <property type="entry name" value="Mitochondrial 2-oxoglutarate/malate carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="16"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="107"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="206"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK07056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="302" mass="32331" checksum="B598C8CA98B4F899" modified="2012-06-13" version="1">MADAKQQQAVAPAGVWKTIKPFVNGGASGMLATCVIQPIDMIKVKIQLGEGSAGQVTKKMLANEGIGSFYKGLSAGLLRQATYTTARLGSFRVLTNKAVEANDGKPLPLVQKAFIGLTAGAIGACVGSPADLALIRMQADSTLPAAQRRHYKNAFHALYRITADEGVLALWKGAGPTVVRAMSLNMGMLASYDQSVELFRDKLGAGEIQTVVGASAISGFFAAACSLPFDYVKTQIQKMQPDANGKYPYTGSLDCALQTFKSGGPFKFYTGFPVYCVRIAPHVMMTWIFLNQIQKFEKKIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HL96</accession>
    <name>I1HL96_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100841221</name>
        <name evidence="5" type="ORF">BRADI_2g34010v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568812.1">
        <property type="nucleotide sequence ID" value="XM_003568764.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231652.1">
        <property type="nucleotide sequence ID" value="XM_010233350.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315061.1">
        <property type="nucleotide sequence ID" value="XM_024459293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HL96"/>
    <dbReference type="EnsemblPlants" id="KQK07220">
        <property type="protein sequence ID" value="KQK07220"/>
        <property type="gene ID" value="BRADI_2g34010v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK07221">
        <property type="protein sequence ID" value="KQK07221"/>
        <property type="gene ID" value="BRADI_2g34010v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK07222">
        <property type="protein sequence ID" value="KQK07222"/>
        <property type="gene ID" value="BRADI_2g34010v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841221"/>
    <dbReference type="Gramene" id="KQK07220">
        <property type="protein sequence ID" value="KQK07220"/>
        <property type="gene ID" value="BRADI_2g34010v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07221">
        <property type="protein sequence ID" value="KQK07221"/>
        <property type="gene ID" value="BRADI_2g34010v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07222">
        <property type="protein sequence ID" value="KQK07222"/>
        <property type="gene ID" value="BRADI_2g34010v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841221"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038211_1_1_1"/>
    <dbReference type="OMA" id="RHRNQVS"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000348">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46225">
        <property type="entry name" value="C3H4 TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46225:SF23">
        <property type="entry name" value="OS05G0179000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="251"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="346"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK07220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="419" mass="46112" checksum="4C88C46897B2DD52" modified="2012-06-13" version="1">MAAPPVESETGSPTDNHPLLMDHLENTSHREIAIDSPRDDDASSSTSSRDNNSGLDRLPRNSENLQETTTPSNSQNAPLARRGDNRVRRQQSPLNSSCWISVELIVTVSQIIAAISVLSLSRNEHPHAPLFEWVIGYTVGCIATLPLLYWRYIHRNRLTTGQESASQNFPPNSIPEANSFTESSASRISEAGHVTGTNGVSQNNTITRNPRVQAYADHFRMALDCFFAVWFVVGNVWIFGGHSSSHDAPNLYRLCIAFLTFSCIGYAMPFILCALICCCLPCIISLMSFREDLNQNKGATAEAINALRTYKFKLKKARNGEGSEGGGILAAGTDKERIVSAEDAVCCICLARYVNNDDLRELPCTHFFHKECVDKWLKINALCPLCKAEIDGGSTSSSALGFGRRHSDNRVVNDIESQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSY7</accession>
    <name>I1HSY7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100844965</name>
        <name evidence="9" type="ORF">BRADI_2g53720v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TOM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564510.1">
        <property type="nucleotide sequence ID" value="XM_003564462.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSY7"/>
    <dbReference type="FunCoup" id="I1HSY7">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HSY7"/>
    <dbReference type="EnsemblPlants" id="KQK10380">
        <property type="protein sequence ID" value="KQK10380"/>
        <property type="gene ID" value="BRADI_2g53720v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844965"/>
    <dbReference type="Gramene" id="KQK10380">
        <property type="protein sequence ID" value="KQK10380"/>
        <property type="gene ID" value="BRADI_2g53720v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844965"/>
    <dbReference type="eggNOG" id="KOG1087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026748_2_1_1"/>
    <dbReference type="OMA" id="QTEIIDM"/>
    <dbReference type="OrthoDB" id="2018246at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14231">
        <property type="entry name" value="GAT_GGA-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03561">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044836">
        <property type="entry name" value="TOL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45898:SF8">
        <property type="entry name" value="OS01G0825700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45898">
        <property type="entry name" value="TOM1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="7">
        <location>
            <begin position="25"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="8">
        <location>
            <begin position="191"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK10380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="602" mass="65503" checksum="E0CF57B30D91E393" modified="2012-06-13" version="1">MYPAGAVAAAAAAPARQAAVRVEKATSHLLLGPDWAVNLEICDVINADVWQTKDAVKAVKKRLRNKDPKVQYYALLLLETMMKNCGEYVQFEVAEQHVLQEMVKIIQKKNDMQVRDKVLLLLDSWQEAFGGPGGKYPQYHWAYLEVKRTGVVFPRHPVDAPPILTPPATHSSQNYGSPRYGAASLNDRMSSDVDTLSLGDLNNIRNVTELLNDMVHALNPSDLKAVKDEIITDLVSKCRSNQQKLMQFVSSTGNEQLLKQGLEINDRLQNVLSKYDAIASGTHLAVEAPVREAAESPQEDPSSKPSAPPIEQNVILNNEEDEFARLAQRKNKSVISSDETSSSAEDLALVPIDMASSESASSVASNALVPLDPVPVSNTQTKEQDMIDLLSLTLCSPTDETCADSSAWNGNEFHQPTVTDGQHNYTGVPQYPSNNQPYSVNEGYTPQNSNYVAPWAQSRAYPSQPPVYATGYPVPPWATPSTVNSNPFQPPAYQEQVPLVASAVSAATHAAPPTSYTSPSASQPTQQYRYVGLPTGDGLTMPQTQMIGNQQPKDSSAAASKPYYILDNLFSDLIDLKSSGGGNIGSSNGGRSMIDEKKIKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1HWD6</accession>
    <name>I1HWD6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase Rpb4/RPC9 core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100843177</name>
        <name evidence="5" type="ORF">BRADI_3g01560v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573316.1">
        <property type="nucleotide sequence ID" value="XM_003573268.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HWD6"/>
    <dbReference type="FunCoup" id="I1HWD6">
        <property type="interactions" value="2960"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HWD6"/>
    <dbReference type="EnsemblPlants" id="KQJ92911">
        <property type="protein sequence ID" value="KQJ92911"/>
        <property type="gene ID" value="BRADI_3g01560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843177"/>
    <dbReference type="Gramene" id="KQJ92911">
        <property type="protein sequence ID" value="KQJ92911"/>
        <property type="gene ID" value="BRADI_3g01560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843177"/>
    <dbReference type="eggNOG" id="KOG2351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110332_1_0_1"/>
    <dbReference type="OMA" id="HRKTQNE"/>
    <dbReference type="OrthoDB" id="2186918at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.40:FF:000004">
        <property type="entry name" value="Dna-directed rna polymerase ii subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045222">
        <property type="entry name" value="Rpb4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21297">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="4">
        <location>
            <begin position="16"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ92911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="141" mass="15950" checksum="43A59E924A989855" modified="2012-06-13" version="1">MSGEEEENAAELKIGEEFLKAKSLMNCEVAIILEHRYEQLQQMSDGGGDPSSQMSQVFEKSLGYVKRFSRYKNPDAVRQVRETLSRYGLHEFELCTLGNLCPDTADEAKALVPSLVPGGRFDVDERIDKMLNDLSLIKKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXB9</accession>
    <accession>A0A0Q3F1I3</accession>
    <name>I1HXB9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC100830520</name>
        <name evidence="25" type="ORF">BRADI_3g04176v3</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">Bd21</strain>
            <strain evidence="26">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 28">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93382.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317324.1">
        <property type="nucleotide sequence ID" value="XM_024461556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXB9"/>
    <dbReference type="EnsemblPlants" id="KQJ93382">
        <property type="protein sequence ID" value="KQJ93382"/>
        <property type="gene ID" value="BRADI_3g04176v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830520"/>
    <dbReference type="Gramene" id="KQJ93382">
        <property type="protein sequence ID" value="KQJ93382"/>
        <property type="gene ID" value="BRADI_3g04176v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_22_9_1"/>
    <dbReference type="OMA" id="GINFMHE"/>
    <dbReference type="OrthoDB" id="647974at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HXB9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000394">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000469">
        <property type="entry name" value="Receptor-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:002651">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF155">
        <property type="entry name" value="LOW QUALITY PROTEIN: RECEPTOR-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003643018" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="34"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="683"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="758"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="787"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KQJ93382.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1043" mass="114219" checksum="98D13C6C58AE4FDE" modified="2012-06-13" version="1">MQTHHCSYWSRFPVAFIGLAVVLLISLPSPTSSCTEQEKSSLLQFLAELSQDGSLTVSWRRNGTDCCTWEGIICGLNGTVTDVSLASRGLEGSISPFLGNLTGLSRLNLSHNLLSGGLPLELVSSSSITVLDVSFNHLTGGLRELPYSTPPRPLQVLNISSNLFTGRFPSTIWEVMKSLVALNASTNSFTGQIPTIPCVSAPSFAVLEISFNEFSGNVPTGLSNCSVLKVLSAGSNNLTGTLPDELFKVTSLEHLSLPGNLLEGALNGIIRLTNLVTLDLGGNDLSGSIPDAIGELKRLEELHLEHNNMSGELPSSLSNCTSLITIDLKSNHFSGELTKVNFSSLPSLKNLDLLYNNFNGTIPESIYTCRNLRALRLSSNNFHGQLSESIGNLKSLSFLSIVNSSLTNITRTLQILRSSRSLTTLLIGFNFMHEAMPEEISTDGFENLQVLAINDCSLSGKIPHWLSKLTNLEMLFLDDNQLTGPIPDWISSLNFLFYLDISNNSLTGEIPSALMDMPMLKSDKTAPKVFELPVYNKSPFMQYLMPSAFPKILNLCMNNFTGLIPEKIGQLKALISLNLSSNTLSGEIPEPISNLTNLQVLDLSGNHLTGTIPAALNNLHFLSKFNISNNDLEGPIPTVGQLSTFTSSSFDGNPKLCGHVLLNNCSSAGTPSIIQKRHTKNSVFALAFGVFFGGVAIIFLLARLLVSLRGKKRSSNNDDIEATSSNFNSEYSMVIVQRGKGEQNKLTVTDLLKATKNFDKEHIIGCGGYGLVYKAELPDGSKVAIKKLNSEMCLMAREFSAEVDALSMAQHDNLVPLWGYCIQGDTRLLIYSYMENGSLDDWLHNRDDDGGSFLDWPTRLKIAQGASRGLSYIHDVCKPHIVHRDIKSSNILLDKEFKAYIADFGLSRLIFHNKTHVTTELVGTLGYIPPEYGQGWVATLRGDMYSFGVVLLELLTGRRPVQICPRSKELVQWVQEMISKEKHIEVLDPTLQGAGHEEQMLKVLEVACRCVNRNPSLRPAIQEVVSALSSRDGNLQKQNSVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXW7</accession>
    <name>I1HXW7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="14">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100839519</name>
        <name evidence="22" type="ORF">BRADI_3g05847v3</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
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        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
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    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
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            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="14"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570586.1">
        <property type="nucleotide sequence ID" value="XM_003570538.4"/>
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    <dbReference type="AlphaFoldDB" id="I1HXW7"/>
    <dbReference type="FunCoup" id="I1HXW7">
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    <dbReference type="STRING" id="15368.I1HXW7"/>
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        <property type="gene ID" value="BRADI_3g05847v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT66016"/>
        <property type="gene ID" value="BRADI_3g05847v3"/>
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    <dbReference type="GeneID" id="100839519"/>
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        <property type="gene ID" value="BRADI_3g05847v3"/>
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        <property type="gene ID" value="BRADI_3g05847v3"/>
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    <dbReference type="KEGG" id="bdi:100839519"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="18">
        <location>
            <begin position="50"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="199"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="19">
        <location>
            <begin position="448"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
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            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
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            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="15">
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            <begin position="683"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
        <location>
            <begin position="1064"/>
            <end position="1154"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022478"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
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        </source>
    </evidence>
    <sequence length="1158" mass="130354" checksum="F6FBCED06DF10729" modified="2012-06-13" version="1">MPPVMPGPDAVAAEGDPDALPVPTYRSLAAPVRRPVDKFELLPAFLKVRGLVKEHIDSFNYFITKGIKNIVQANNRIEARSDPSIYLRYKDIRVGEPSVQVDFRVETITPHFCRLTDRTYSAPVIVDIEYTVGKTHVVHRKPNFIIGYMPIMLRSYACVLNGKDEAELARYGECPLDPGGYFVVKGNEKVILIQEQLSKNRIIIDTDNKKRVIASVTSSTHEVKSKTVIVMDKEKIYLQLNQFTKPIPIIVVMKAMGIQSDQEIVQMVGRDPRYGDLLYLSIQECASERIYTQQQALQYMDDKVMFPGPGNVKEGRSKTLLRDVFVAHVPVENGNFREKCIYTAVMLRRMLDAILNSDTFDDKDYVGNKRLELSGQLVSLLFEDLFKTMNSFAVDRMNKNSDMARSSPLDFSQLIMQQDLITTGLERAISTGNWDIKRFKMHRKGVSQVLSRLSYMASLGYMTRITPQFEKTRKTSGPRALQPSQWGMLCPCDTPEGEACGLTKNLALLTHVTTDQEEGPLMNLCYSLGVEKLSLLSGEEIHAPGSFLVMFNGLILGKHRQPQRFAKSMRTFRQSGKIGEFVSIFVNEKQHCIHIASDGGRVCRPLIIADKGRSRVKEHHMKELRDGVRSFDDFLRDGLIEYLDVNEENNALIALYEHLDQDDVQRSSITHIEIEPMTILGVVAGLIPYPHHNQSPRNTYQCAMGKQAMGNIAYNQLFRADSLLYLLVYAQRPLLTTKTIELVGYDKLGAGQNATVAVMSYSGYDIEDAIVMNKSSLDRGFGRCIAMKKYTVTTEKYANGTISDRIAKPQRDKDGALIKQNMRALDEDGFVAPGQIIRNHDIYVNKQTPKAIPKTPGTTLTDRDYKDSPAVYKGVDGETTVVDRVMLCSDTNDKLTIKCIIRHTRRPEVGDKFSSRHGQKGVCGTIVQQEDFPFSERGICPDLIMNPHGFPSRMTIGKMIELLGGKAGVSCGRFHYGSAFGESSGNADHVDDISHTLVKHGFSYNGKDFLYSGILGHPLEAYIFMGPIYYQKLKHMVLDKMHARASGPRVLLTRQPTEGRSRDGGLRLGEMERDCLIAYGASMLIFERLLLSSDPYQVQVCRKCGLLGYYNYKLKTSFCSMCKNGENMAKMRMPYACKLLFQELQSMNVVPRLKLTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZG5</accession>
    <name>I1HZG5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100841858</name>
        <name evidence="4" type="ORF">BRADI_3g10127v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572383.1">
        <property type="nucleotide sequence ID" value="XM_003572335.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZG5"/>
    <dbReference type="FunCoup" id="I1HZG5">
        <property type="interactions" value="614"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HZG5"/>
    <dbReference type="EnsemblPlants" id="KQJ94359">
        <property type="protein sequence ID" value="KQJ94359"/>
        <property type="gene ID" value="BRADI_3g10127v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841858"/>
    <dbReference type="Gramene" id="KQJ94359">
        <property type="protein sequence ID" value="KQJ94359"/>
        <property type="gene ID" value="BRADI_3g10127v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841858"/>
    <dbReference type="eggNOG" id="KOG0975">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020844_0_0_1"/>
    <dbReference type="OMA" id="MSRIAYV"/>
    <dbReference type="OrthoDB" id="25921at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HZG5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000002">
        <property type="entry name" value="D-alanine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000008">
        <property type="entry name" value="D-amino-acid transaminase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF22">
        <property type="entry name" value="D-AMINO-ACID TRANSAMINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ94359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="393" mass="42036" checksum="BDED9C0983BB3621" modified="2012-06-13" version="1">MGHLSALPPPAASGHRAYRPFLAQTKIAPSPGGGAGAAPDGLLLWRAAVAIAARRSNWAASAGTIVNPIDVPLLSFSEVAERLEAFQASGARKQNYMAMYSSIFGGITTDPSAMVIPIDDHMVHRGHGVFDTAAIMDGHIYELQQHLDRFLKSAQMAKIQLPFDRSRIQSVLIQTVCASKCTQGSLRYWLSVGPGDFQLSSLGCTNPALYAIVIDSPSLPVPSGCKVVTSSIPMKSPQFAVMKNVNYLPNALTKVEGEENGGFTGIWLDDEGFVAEGSNMNVGFVTRNKELILPRFDNILSGCTAKRVLDLAGQLVDDGRLSGITTRNVSVQEGKAADEMMLIGSGILVKPVVQWDEQMVGSGKEGPIAQALFNLILEDMRSGPPSVRIAVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1I006</accession>
    <name>I1I006_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100835861</name>
        <name evidence="8" type="ORF">BRADI_3g12030v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573207.1">
        <property type="nucleotide sequence ID" value="XM_003573159.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234193.1">
        <property type="nucleotide sequence ID" value="XM_010235891.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756602.1">
        <property type="nucleotide sequence ID" value="XM_014901116.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I006"/>
    <dbReference type="STRING" id="15368.I1I006"/>
    <dbReference type="EnsemblPlants" id="KQJ94649">
        <property type="protein sequence ID" value="KQJ94649"/>
        <property type="gene ID" value="BRADI_3g12030v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ94650">
        <property type="protein sequence ID" value="KQJ94650"/>
        <property type="gene ID" value="BRADI_3g12030v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ94651">
        <property type="protein sequence ID" value="KQJ94651"/>
        <property type="gene ID" value="BRADI_3g12030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835861"/>
    <dbReference type="Gramene" id="KQJ94649">
        <property type="protein sequence ID" value="KQJ94649"/>
        <property type="gene ID" value="BRADI_3g12030v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ94650">
        <property type="protein sequence ID" value="KQJ94650"/>
        <property type="gene ID" value="BRADI_3g12030v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ94651">
        <property type="protein sequence ID" value="KQJ94651"/>
        <property type="gene ID" value="BRADI_3g12030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835861"/>
    <dbReference type="eggNOG" id="KOG0459">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="ARFDECT"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I006">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018444">
        <property type="term" value="C:translation release factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000024">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit ERF3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000862">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit ERF3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000077">
        <property type="entry name" value="Putative translation elongation/initiation factor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="99"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ94649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="542" mass="59438" checksum="793D593523C75EFB" modified="2012-06-13" version="1">MEHDAEMASPISSDMVTAADDWAARDDFEDSPAESHAHPPAGAGAAAAVTEDEAREEAPPAPPSEDANGIESTLQSLELQPSDAPHNRVQIEAEERNRKRHLNVVFIGHVDAGKSTAGGQILFLSGQVDDRTIQKYEKEAKDKSRESWYMAYIMDTNEEERAKGKTVEVGRAHFETETTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYERGGQTREHVLLAKTLGVSKLIVVINKMDDPTVGWSKERYDEIEGKMTPFLKSSGYNVKKDVQFLPISGLLGSNMKTRLDKSICSWWNGPCLFELMDCIEVPLRDPKGPVRLPIMDKYKDMGTVVMGKIESGTISEGDNLVIMPNKANVKVISVYCDEDKVRSASPGENVRVKLSGIEEEDIAAGFVLSNIGNPVGAVSEFNAQLQILELLDNAIFTAGYKAVLHIHSVVEECEIVELIEEIDLKKKKESDPKKRKPKKKPLFVKNGAVVVCRIQVSNLICIENFSDSPQLGRFTLRTEGKTIAVGKVVAVPPTGISTFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1I359</accession>
    <name>I1I359_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">(S)-ureidoglycine aminohydrolase cupin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100824300</name>
        <name evidence="2" type="ORF">BRADI_3g21670v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <strain evidence="2 3">Bd21</strain>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="162" mass="17647" checksum="75AC251DA735F702" modified="2012-06-13" version="1">MASTAGAGAGARCHLLSPIPMAAGAAAGASSRRRRCVLSAASGGGGGGRMVGRRPIEDVYKVRVERGATAQARVEALRVMETWSAWRTGGRCHMPWDWQVDQLVYVVSGEVRVLPTGATTGEEYMHFVAGDLVRYPKWLEADLHFDGPYEERYRFLAYGDDN</sequence>
</entry>
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    <accession>I1I582</accession>
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    </gene>
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        <lineage>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblPlants" id="KQJ97368">
        <property type="protein sequence ID" value="KQJ97368"/>
        <property type="gene ID" value="BRADI_3g30320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829714"/>
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        <property type="protein sequence ID" value="KQJ97368"/>
        <property type="gene ID" value="BRADI_3g30320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829714"/>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="LTI65/LTI78_PGEED"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR037491">
        <property type="entry name" value="LTI78/LTI65"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33836">
        <property type="entry name" value="LOW-TEMPERATURE-INDUCED 65 KDA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33836:SF1">
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    </feature>
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5Z6</accession>
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    <reference evidence="5 6" key="1">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
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                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="5">Bd21</strain>
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    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
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    <dbReference type="eggNOG" id="ENOG502QV6R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019073_4_1_1"/>
    <dbReference type="OMA" id="HMYSAEK"/>
    <dbReference type="OrthoDB" id="1053009at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I5Z6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2010:FF:000001">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2010:FF:000003">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1310:FF:000002">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2010">
        <property type="entry name" value="PRONE domain, subdomain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005512">
        <property type="entry name" value="PRONE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038937">
        <property type="entry name" value="RopGEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101">
        <property type="entry name" value="ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101:SF47">
        <property type="entry name" value="ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03759">
        <property type="entry name" value="PRONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51334">
        <property type="entry name" value="PRONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRONE" evidence="4">
        <location>
            <begin position="96"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ97726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="495" mass="52884" checksum="1800DD6F25E3EEFE" modified="2012-06-13" version="1">MADSSISSPSLSASDESSELDAGCYSSSTTTAPPSLPDTADFSRSASDASSFSDHSGPFGAAAVSKLIAGRGSPAGAGASLRRLSMKPRADVLDRRSATAADVDELELVKERFSKLLLGEDMSGGGKGVCAAVAISNAITNLYATVFGSCCHRLEPLPEGKKAMWRREMDCLLSVCDHIVEFYPSSQALPDGTRVEVMATRPRSDIYINLPALEKLDAMLIEIMDGFEKAEFWYADDGGARSFGSTATTTTSSSASPSPASSFRRSSAALHRKNEDKWWVPVPCVPDGGLSAAARKELRRRRDCASQIHKAAVAINSDVLGDMEVPESFMALLPKSGKASVGDAVYRAMMGGGGGKFSPDHLLDCVDVSSEHEALALADRVEAAMYVWRRKASASLAHGGRWVQWSKVKELAADDGGDGGKNMTLASRAESLLLCIKHRFPGLSQTTLDTSKIQFNKDVGQAILESYSRVLESLAFSIVSWIDDVLFADKSVRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>I1I9F1</accession>
    <name>I1I9F1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100823393</name>
        <name evidence="12" type="ORF">BRADI_3g42750v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574923.1">
        <property type="nucleotide sequence ID" value="XM_003574875.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235404.1">
        <property type="nucleotide sequence ID" value="XM_010237102.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I9F1"/>
    <dbReference type="STRING" id="15368.I1I9F1"/>
    <dbReference type="EnsemblPlants" id="KQJ99346">
        <property type="protein sequence ID" value="KQJ99346"/>
        <property type="gene ID" value="BRADI_3g42750v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823393"/>
    <dbReference type="Gramene" id="KQJ99346">
        <property type="protein sequence ID" value="KQJ99346"/>
        <property type="gene ID" value="BRADI_3g42750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823393"/>
    <dbReference type="eggNOG" id="KOG0882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_31_2_1"/>
    <dbReference type="OMA" id="GMVEYWR"/>
    <dbReference type="OrthoDB" id="10264753at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048440">
        <property type="term" value="P:carpel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010338">
        <property type="term" value="P:leaf formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010358">
        <property type="term" value="P:leaf shaping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010305">
        <property type="term" value="P:leaf vascular tissue pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009933">
        <property type="term" value="P:meristem structural organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010082">
        <property type="term" value="P:regulation of root meristem growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048453">
        <property type="term" value="P:sepal formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048443">
        <property type="term" value="P:stamen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd01927">
        <property type="entry name" value="cyclophilin_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000003">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000450">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD-repeat protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="87"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="11">
        <location>
            <begin position="495"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ99346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="652" mass="73050" checksum="E9659C38F76A1A6D" modified="2012-06-13" version="1">MATTSDPTADPALPTETAEGDAVQEQEHGNGAAAAPAVVLQQQGQGEEEEELIGPGPAPARQRQKRPLQFEQAFLDALPSAAMYEKSYMHRDVVTHVAVSPADFFITGSADGHLKFWKKKPSGIEFAKHFRSHLSPIEGLAVSVDGLLCCTISNDRSVKIYDVVNYDMMFMMRLPFVPGAIEWVYRQGDVKPKLAVSDRNTSFIHVYDTHSGSVDPVISKEIHSGPVKVMKYNHAQDVVVSADAKGLLEYWSPSTLKFPEDVVNFRLKTDTNLFELAKCKTSVSAIEVSNDGTQFAVTSPDRRIRVFWFKTGKLRRVYDESLEVAQDLQKSDVPLYHLDAIDFGRRMAVEKEIEKTENVPQPNAVFDESSNFLIYATLLGIKVVNLHTNKVSRILGKVENNERFLRIALYQGDKGNKKVRKIPSIAANVNDSKEPLSDPTLLCCAFKKHRIYTFSRREPEEPEDATKGRDVFNEKPPPEELLSVSELGKTATTSLPDNLVFHTSMGDIHLKLYPEECPKTVENFTTHCRNGYYDNLIFHRVIKGFMVQTGDPLGDGTGGQSIWGREFEDEFHKSLRHDRPFTLSMANAGPNTNGSQFFITTVATPWLDNKHTVFGRVVKGMDVVQQIEKVKTDKNDKPYQDVKILNVTVPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>I1I9J4</accession>
    <name>I1I9J4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="11">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100831056</name>
        <name evidence="13" type="ORF">BRADI_3g43070v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="11">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="11"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574947.1">
        <property type="nucleotide sequence ID" value="XM_003574899.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I9J4"/>
    <dbReference type="FunCoup" id="I1I9J4">
        <property type="interactions" value="1354"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I9J4"/>
    <dbReference type="EnsemblPlants" id="KQJ99399">
        <property type="protein sequence ID" value="KQJ99399"/>
        <property type="gene ID" value="BRADI_3g43070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831056"/>
    <dbReference type="Gramene" id="KQJ99399">
        <property type="protein sequence ID" value="KQJ99399"/>
        <property type="gene ID" value="BRADI_3g43070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831056"/>
    <dbReference type="HOGENOM" id="CLU_056632_1_0_1"/>
    <dbReference type="OrthoDB" id="2015551at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I9J4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000003">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn], chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="8 11" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="12">
        <location>
            <begin position="65"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ99399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="204" mass="20634" checksum="263D9C2584FFD4EC" modified="2012-06-13" version="1">MASVTAAAQTTLLSAATAPTSLFQAAPSSARPFHPLRLVSAGRRTLVVADATKKAVAVLKGTSQVEGVVTLTQEDDGPTSVNVRITGLTPGLHGFHLHEFGDTTNGCISTGPHFNPNGLTHGAPGDEVRHAGDLGNIVANAEGIAETTIVDSQIPLSGPNAVVGRAFVVHELEDDLGKGGHELSLSTGNAGGRLACGVVGLTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICG9</accession>
    <name>I1ICG9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100836341</name>
        <name evidence="2" type="ORF">BRADI_3g51300v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235805.1">
        <property type="nucleotide sequence ID" value="XM_010237503.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICG9"/>
    <dbReference type="EnsemblPlants" id="KQK00706">
        <property type="protein sequence ID" value="KQK00706"/>
        <property type="gene ID" value="BRADI_3g51300v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836341"/>
    <dbReference type="Gramene" id="KQK00706">
        <property type="protein sequence ID" value="KQK00706"/>
        <property type="gene ID" value="BRADI_3g51300v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836341"/>
    <dbReference type="eggNOG" id="ENOG502S5QT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1888998_0_0_1"/>
    <dbReference type="OMA" id="RCPRIMV"/>
    <dbReference type="OrthoDB" id="1304043at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK00706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="131" mass="13954" checksum="722C84DE57742E92" modified="2012-06-13" version="1">MATRCFFPRDAAGGHGQSKAAAEALEQLHNGGRVLSREDVGGAVRVKIVVSKRELKKMVAALGAGAGGEAAAAPGDRRGGRQRAAAGTTDAEQRLLSLRRRSMRRAAEETRRMQASGEWEPGLQSIPEEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>I1IE20</accession>
    <name>I1IE20_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Ethylene receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">LOC100824308</name>
        <name evidence="28" type="ORF">BRADI_3g55730v3</name>
    </gene>
    <organism evidence="29">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">Bd21</strain>
            <strain evidence="29">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="28">Bd21</strain>
        </source>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="29">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">May act early in the ethylene signal transduction pathway, possibly as an ethylene receptor, or as a regulator of the pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="20">Binds 1 copper ion per dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the ethylene receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235987.1">
        <property type="nucleotide sequence ID" value="XM_010237685.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IE20"/>
    <dbReference type="STRING" id="15368.I1IE20"/>
    <dbReference type="EnsemblPlants" id="KQK01419">
        <property type="protein sequence ID" value="KQK01419"/>
        <property type="gene ID" value="BRADI_3g55730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824308"/>
    <dbReference type="Gramene" id="KQK01419">
        <property type="protein sequence ID" value="KQK01419"/>
        <property type="gene ID" value="BRADI_3g55730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824308"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_48_1"/>
    <dbReference type="OMA" id="GRHEMEI"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051740">
        <property type="term" value="F:ethylene binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038199">
        <property type="term" value="F:ethylene receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010105">
        <property type="term" value="P:negative regulation of ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16938">
        <property type="entry name" value="HATPase_ETR2_ERS2-EIN4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000004">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014525">
        <property type="entry name" value="ETR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058544">
        <property type="entry name" value="ETR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24423:SF624">
        <property type="entry name" value="ETHYLENE RECEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24423">
        <property type="entry name" value="TWO-COMPONENT SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25487">
        <property type="entry name" value="ETR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026389">
        <property type="entry name" value="Ethyln_sen_HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="24" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14 19 20" id="KW-0186">Copper</keyword>
    <keyword evidence="17 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 19" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="10 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 19 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="26" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15 19" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095243" description="Ethylene receptor" evidence="26">
        <location>
            <begin position="43"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="27">
        <location>
            <begin position="655"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="21">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="21">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)" evidence="22">
        <location>
            <position position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR026389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR026389-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR026389-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR026389-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="KQK01419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="774" mass="86067" checksum="7CD2B3B1119D20A8" modified="2012-06-13" version="1">MLCWMVPPRFRCSRRGRRSSPTAAMRVLLLLLLAVAPAAVAATGDDVGYPHCNCGGGGGFWSMENIFKWQKVSDLLIAAAYFSIPLEILYFVAGLRHLLPFRWVLVQFGAFIVLCGLSHLLTAFTYEPHPFMVVLLLTTAKFLTALVSFLTAITLLTLIPQLLRVKVRESLLWIKARELDREVDLMKQQEEASWHVRMLTQEIRKSLDRHTVLYTTLIELSRVLELKNCAVWMPAEDKAAMHLTHELRRGGSDGLVVGVDDADVVEVRRSDGVKLLGPDSVLASASGGGREDTGPVAAIRMPMLKVSDFKGGTPEVIQASYAVLVLVPPSDKNWGAHELEIVEVVADQVAVALSHASLLEESQAMRDRLAEQNRELLQARRDTLMANEARDAFQLVMSQGMRKPIHSILGLVSVVQEDNLMPEQKLVVNTMARTATVVSTLINDVMEMSATNRERFPLETRPFHLHSMIRDAACVARCLCDFRGFGFAVHVENTLPDLVIGDERRIFHVILHMVGNLISRINSGHVTFRVRADDEVMEGSLGQRWDPWRPSYSSGYSSVKFVIGWKSTESVSSAGQFLRKPNGEGFDLRLSFSMCRKLVQMMRGNIWAVLDGQGLPESMTLVLRFQLQPSLTSSSTGGSFERQYTSPSCQLTGLKVLLIDDDDINMVVARKLLEKLGCVVSSLPSGSGFLNSVGPSSGAFQVVMVNLEMTRVNALDVAARVRQYRSGRWPFVMAMTSEQNAWEKCAQSGINGIIRKPVVLREMKEELTRILQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IFR0</accession>
    <name>I1IFR0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100830518</name>
        <name evidence="14" type="ORF">BRADI_3g60590v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK02140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570724.1">
        <property type="nucleotide sequence ID" value="XM_003570676.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IFR0"/>
    <dbReference type="FunCoup" id="I1IFR0">
        <property type="interactions" value="2399"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IFR0"/>
    <dbReference type="EnsemblPlants" id="KQK02140">
        <property type="protein sequence ID" value="KQK02140"/>
        <property type="gene ID" value="BRADI_3g60590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830518"/>
    <dbReference type="Gramene" id="KQK02140">
        <property type="protein sequence ID" value="KQK02140"/>
        <property type="gene ID" value="BRADI_3g60590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830518"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IFR0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:RNA helicase activity"/>
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        <property type="term" value="P:mRNA splicing, via spliceosome"/>
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        <property type="entry name" value="DEADc_EIF4AIII_DDX48"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATP-dependent RNA helicase DBP5"/>
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        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
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    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
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    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
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    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="68"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="249"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK02140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="410" mass="45814" checksum="14A7397707EF1ED5" modified="2012-06-13" version="1">MAAAGATTSRRGPGGGGGAMDDENLTFETSAGVEVVTSFDAMGIRDDLLRGIYGYGFDKPSAIQQRAVLPIITGRDVIAQAQSGTGKTSMISLSVCQIVDTSVHEVQALILSPTRELATQTEKVMQAVGNYMSVNVHACVGGKSIGEDIRKLESGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLDEADEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHDILEITSKFMTDPVRILVKRDELTLEGIKQFFVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTERMRTNNFTVSAMHGDMPQKERDAIMNEFRGGSTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVRKDDIRILRDIEQYYSTQIDEMPMNVADLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJK8</accession>
    <name>I1IJK8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100832373</name>
        <name evidence="3" type="ORF">BRADI_4g10710v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003577216.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJK8"/>
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        <property type="interactions" value="1068"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IJK8"/>
    <dbReference type="EnsemblPlants" id="KQJ87392">
        <property type="protein sequence ID" value="KQJ87392"/>
        <property type="gene ID" value="BRADI_4g10710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832373"/>
    <dbReference type="Gramene" id="KQJ87392">
        <property type="protein sequence ID" value="KQJ87392"/>
        <property type="gene ID" value="BRADI_4g10710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832373"/>
    <dbReference type="eggNOG" id="KOG0431">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007921_0_0_1"/>
    <dbReference type="OMA" id="LMFNDFY"/>
    <dbReference type="OrthoDB" id="1717591at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072318">
        <property type="term" value="P:clathrin coat disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000009">
        <property type="entry name" value="Auxilin-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172">
        <property type="entry name" value="AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172:SF50">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="707"/>
            <end position="733"/>
        </location>
    </feature>
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        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
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        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="457"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="532"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="667"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="717"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ87392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="857" mass="93833" checksum="F4448A869A9E7EC8" modified="2012-06-13" version="1">MDGIEGLLASNFGVRPQGKAAPMAAAASSSRGGSSSGSAWPNPRSTPASAPSYDDLFGAPVPVPAPASAAASSPSLDSLFDSFKPPAPSSSSAAAARSKPAFDDDDIFSAVPGLRPTNSASSATRYDGDDVFGAGTAGPVYDDVFVSSNPRSAPQHSSYDDLLGGLAGRPRAEERKGSVAVEDDDLLGGFGMMPPPASEKKKPVVAEEDSQGSNGFDDLIPGFASTSPPKSRKIDDDNKKKPAVPTSKSTPNKNFDSTADDESLFDDSSTFDQVPKSDPLFTSEINGTKDSHQNSNSVQSSVNRNPASRSSLEDFGDVMPKSQSTRYSDIHVDDSSERYSGNSMEDQSPISTESEDDIWLTVSEIPLFTRPTSAPPPSRSPPRLKQKSLGAKANGKYDEYVTQSTQSRNHYKDLPKQADISSTSEWGGVAMDKPQMPANYDNNSFDDDEEFNTNSAAREEAESQERLEQAQEMRQREEQRRLEKERELEQQREKQAVERATKEARDRAAAEARAKAERDARQRSQRAAVQRAHQEARERAAAEAKERVARAAAEERERAATEAKERERAAAEAKERERTVSRDKAAAERAAVERVQQEARKRAERAATERAAAEARERHAAAAAAAAKEKQSTPVDVESFFRDSAPKQRAPTVDSTFDPQPRGRGTVNGSQRAAPTSASTRKANTSSATNMDNLFNLFDDQVPASSNVFQDVEGESEERRRARLERHQRTNERVEKALAEKNERDMQVQREQAERDRIGDSLDFEIKRWAAGKEGNLRALLSTLQYILWPACGWQAVSLTDLITGAAVKKQYRKATLCIHPDKVQQKGATLQQKYIAEKVFDILKEAWNKFNSEELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMF2</accession>
    <name>I1IMF2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g21860v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IMF2"/>
    <dbReference type="EnsemblPlants" id="KQJ88877">
        <property type="protein sequence ID" value="KQJ88877"/>
        <property type="gene ID" value="BRADI_4g21860v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88877">
        <property type="protein sequence ID" value="KQJ88877"/>
        <property type="gene ID" value="BRADI_4g21860v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_21_3_1"/>
    <dbReference type="InParanoid" id="I1IMF2"/>
    <dbReference type="OMA" id="ILESWSI"/>
    <dbReference type="OrthoDB" id="187462at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707">
        <property type="entry name" value="C2 CALCIUM/LIPID-BINDING PLANT PHOSPHORIBOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707:SF77">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ88877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="146" mass="16146" checksum="FAB6206C67B49A2D" modified="2012-06-13" version="1">MQSRAITQKVSGTMGYFAPEYLSGVITYHVDLYSLGVTIMEILTGEKGYHDVENVVECWSNGSKKSQSDLQLEQVRVCAEIGIQCTDFNPAKRPDTQHIIGRLCETESMDEHIGIGVITSQLAENDPIELDKDSRMTLGETSSEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-04-02" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1INW6</accession>
    <name>I1INW6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_4g26730v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INW6"/>
    <dbReference type="STRING" id="15368.I1INW6"/>
    <dbReference type="EnsemblPlants" id="KQJ89611">
        <property type="protein sequence ID" value="KQJ89611"/>
        <property type="gene ID" value="BRADI_4g26730v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89611">
        <property type="protein sequence ID" value="KQJ89611"/>
        <property type="gene ID" value="BRADI_4g26730v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122115_0_0_1"/>
    <dbReference type="InParanoid" id="I1INW6"/>
    <dbReference type="OMA" id="CRNSMAT"/>
    <dbReference type="OrthoDB" id="682531at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15570">
        <property type="entry name" value="PHD_Bye1p_SIZ1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="5">
        <location>
            <begin position="38"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ89611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="120" mass="12980" checksum="AABD87E36923B29F" modified="2012-06-13" version="1">MHDPVNALSVSGSQIEPGHGVKPKKKPDDSTQLAVMVRCPCRNSMATGSMIKCDNPQCNVWQHVDCVIIPKEPGDSVPPELPSSFYCDMCRISRADPFWVTINHPLLPAAITPSIAALDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQR0</accession>
    <name>I1IQR0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 14">Alpha-amylase</fullName>
            <ecNumber evidence="11 14">3.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">1,4-alpha-D-glucan glucanohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100845943</name>
        <name evidence="17" type="ORF">BRADI_4g32140v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11 14">
            <text>Endohydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1-&gt;4)-alpha-linked D-glucose units.</text>
            <dbReference type="EC" id="3.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 13">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.1" evidence="11 14"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578230.1">
        <property type="nucleotide sequence ID" value="XM_003578182.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQR0"/>
    <dbReference type="FunCoup" id="I1IQR0">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IQR0"/>
    <dbReference type="EnsemblPlants" id="KQJ90515">
        <property type="protein sequence ID" value="KQJ90515"/>
        <property type="gene ID" value="BRADI_4g32140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845943"/>
    <dbReference type="Gramene" id="KQJ90515">
        <property type="protein sequence ID" value="KQJ90515"/>
        <property type="gene ID" value="BRADI_4g32140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845943"/>
    <dbReference type="eggNOG" id="KOG0471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030069_1_0_1"/>
    <dbReference type="OMA" id="WESCKNQ"/>
    <dbReference type="OrthoDB" id="550577at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005983">
        <property type="term" value="P:starch catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11314">
        <property type="entry name" value="AmyAc_arch_bac_plant_AmyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000021">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012850">
        <property type="entry name" value="A-amylase_bs_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013775">
        <property type="entry name" value="A-amylase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006046">
        <property type="entry name" value="Alpha_amylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43447">
        <property type="entry name" value="ALPHA-AMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07821">
        <property type="entry name" value="Alpha-amyl_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001028">
        <property type="entry name" value="Alph-amls_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00110">
        <property type="entry name" value="ALPHAAMYLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00810">
        <property type="entry name" value="Alpha-amyl_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 14" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0309">Germination</keyword>
    <keyword evidence="10 14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013989121" description="Alpha-amylase" evidence="11">
        <location>
            <begin position="27"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="15">
        <location>
            <begin position="29"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-amylase C-terminal beta-sheet" evidence="16">
        <location>
            <begin position="369"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001028-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ90515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="439" mass="47569" checksum="81D70C25FC12C351" modified="2012-06-13" version="1">MGKQVTALNGLLVAMALLCLSCDALAQTQILFQGFNWESCNKQGGWYNMLKGRVGSIASSGVTHVWLPPPSHSVSPQGYMPGRLYDVDASKYGTKAELKSLIAAFHGKGVKCVADIVINHRTAEEKDGRGVYCIFKGGGGPEGQLDWGPGMICSDDAKYSDGTGHRDTGADFAAAPDIDHLNPRVQRELSAWLGWLKSDLGFDGWRLDFAKGYSAEVAKVYAGNASPGFVVAEIWNSLSYDGDGKPATNQDGERQELVNWAKAVGGPAMAFDFTTKGILQAAVQGELWRMRGKDGKAPGMIGLSPEKAVTFVDNHDTGSTQKMWPFPADKVMMGYAYILTHPGLPCIFYDHVFDWNLKKEIDTLAAVRSRNGINAGSKLRILAAESDVYVAMVDEKVISKIGPRFDAGNLIPSDFHVVAHGSNYCVWEKSGLRVPAGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IT05</accession>
    <name>I1IT05_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="4">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FAR1</name>
        <name evidence="9" type="synonym">LOC100827426</name>
        <name evidence="8" type="ORF">BRADI_4g38460v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>Three Fatty Acyl-Coenzyme A Reductases, BdFAR1, BdFAR2, and BdFAR3, are involved in primary alcohols biosynthesis of cuticular wax in Brachypodium distachyon.</title>
            <authorList>
                <person name="Wang Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="4"/>
    <dbReference type="EMBL" id="MF285084">
        <property type="protein sequence ID" value="ASK86469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578677.1">
        <property type="nucleotide sequence ID" value="XM_003578629.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IT05"/>
    <dbReference type="STRING" id="15368.I1IT05"/>
    <dbReference type="EnsemblPlants" id="KQJ91581">
        <property type="protein sequence ID" value="KQJ91581"/>
        <property type="gene ID" value="BRADI_4g38460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827426"/>
    <dbReference type="Gramene" id="KQJ91581">
        <property type="protein sequence ID" value="KQJ91581"/>
        <property type="gene ID" value="BRADI_4g38460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827426"/>
    <dbReference type="eggNOG" id="KOG1221">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024661_4_1_1"/>
    <dbReference type="OMA" id="VWKMIET"/>
    <dbReference type="OrthoDB" id="429813at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010345">
        <property type="term" value="P:suberin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF44">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="6">
        <location>
            <begin position="21"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="5">
        <location>
            <begin position="422"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ASK86469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ91581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="527" mass="58313" checksum="C9E622DC87C8CDC0" modified="2012-06-13" version="1">MVGTLDEGMIIEYFKNKSILITGSTGFLGKILVEKILRVQPDVKKIYLPVRAVDAGAARRRVQTEVLEKELFGLLREKHGQAGFDRFVESKIVALAGDVMREGFGIDGATSAELGLLESLHVIVNGAATTNFYERYDVALDVNVQGVKHMCDFAKNCPNLEALLHVSTAYVAGEKQGLVRERTFRDGETLREGTHLDIGAELRLARDLKKQLEYVDGPAAEKAKAERKAMKELGLARARHFGWPNTYVFTKSMGEMMLAQQLRGCGVPVVIVRPSIITSVLNDPLPGWIEGTRTIDAILIGYAKQNLSCFLADLDLTMDVMPGDMVVNAMMAATAAAAHSNGGPNAVNPAHSPSPPQPQPVTVYHVSSSLRNPAPYAVLYRTGIRYFTEHPRRTADGRVAPTRKVRFLSTLASFRLFMLLRYRLPLELLHLLAFLCCGLFGLAALYDRLARRYRFVMQLVDLYGPFSLFKGCFDDVNLSKLRVAMDNGGNGGGGEGQGVFNFDAKTVDWDDYFYRVHIPGVMKYVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVC8</accession>
    <name>I1IVC8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100844423</name>
        <name evidence="3" type="ORF">BRADI_4g45410v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310531.1">
        <property type="nucleotide sequence ID" value="XM_024454763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVC8"/>
    <dbReference type="FunCoup" id="I1IVC8">
        <property type="interactions" value="1034"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IVC8"/>
    <dbReference type="EnsemblPlants" id="KQJ92710">
        <property type="protein sequence ID" value="KQJ92710"/>
        <property type="gene ID" value="BRADI_4g45410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844423"/>
    <dbReference type="Gramene" id="KQJ92710">
        <property type="protein sequence ID" value="KQJ92710"/>
        <property type="gene ID" value="BRADI_4g45410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844423"/>
    <dbReference type="eggNOG" id="ENOG502QUHX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028932_0_0_1"/>
    <dbReference type="OMA" id="FSHVWRT"/>
    <dbReference type="OrthoDB" id="385235at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IVC8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035770">
        <property type="term" value="C:ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901259">
        <property type="term" value="P:chloroplast rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000963">
        <property type="term" value="P:mitochondrial RNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044528">
        <property type="term" value="P:regulation of mitochondrial mRNA stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.960.10">
        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="2">
        <location>
            <begin position="549"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="614" mass="68822" checksum="34D71FB512973836" modified="2012-06-13" version="1">MAAPVDAASSFQPKLGFLLVRRPPQALLLLPRLAHTIPNLAVNPCRASVKEDDATPQWQLDFLGPHPQPRPDEEEDDDPLPAESTDWCVRARRSALRSIEARGLSPSLQRMVSPPKKISNNKKRKKQKKILDKKKKKNDELTDEEDEMDSDAVPDDLDHRVAQLADGVFDEKRQRNRELFIQTLSSFSAAQPSNRSKEVSLNRDIVQARTAEEVLALTAEVMAAVAKGLSPSPLTPLNIATALHRIAKNMETVSMTQTHRLAFARQRDMSMLVGLAMLSLPECSPQGVSNISWALSKIGGDLLYLPEMDRIAKVAISKVDDFNAQNVANVAGAFASMRQSAPALFLALAQRAAQLVYTFKEQELAQFLWGCASLNECPYPLLDALDAAFQDGLVSDMRQTSAKDASSGEDVSNAHALSFSRDQLGNIAWSYTVLGQIDRQFFSHIWKTLKQYEEQRVSDQYREDIMFASQVYLANQSVKLEYPHLDFALRGDLEEKITKAGKSKRFNQKTTSSFQKEVGHLLYITGHEWIREYTVDGYTLDAVLVDEKVALEIDGTTHFSRNLGTPLGHTALKRRYITTAGWKLVSLSHQEWEELQGESEQMEYLRRILGIDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0Y8</accession>
    <name>I1J0Y8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100843120</name>
        <name evidence="7" type="ORF">BRADI_5g19400v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily. Tau family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580393.3">
        <property type="nucleotide sequence ID" value="XM_003580345.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0Y8"/>
    <dbReference type="STRING" id="15368.I1J0Y8"/>
    <dbReference type="EnsemblPlants" id="PNT61713">
        <property type="protein sequence ID" value="PNT61713"/>
        <property type="gene ID" value="BRADI_5g19400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843120"/>
    <dbReference type="Gramene" id="PNT61713">
        <property type="protein sequence ID" value="PNT61713"/>
        <property type="gene ID" value="BRADI_5g19400v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0406">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_18_0_1"/>
    <dbReference type="OMA" id="RECSEGK"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000044">
        <property type="entry name" value="Glutathione S-transferase GSTU6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000023">
        <property type="entry name" value="Probable glutathione S-transferase GSTU6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF511">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="91"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT61713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="237" mass="25477" checksum="CAD8158EF174ECF6" modified="2012-06-13" version="1">MAGGDELKLLGSWASPFVTRAKLALALKGLSYEDVEEDLQNKSELLLSSNPVHKKVPVLIHNGVPVCESMLIVQYIDEAFAGTGPSVLPADPYERAIARFWAAYVDDKLVASWVKSSKSNTEEDKAEAMKQTFVAVEALEGALRECSKGEGGFFGGDSVGLVDISLGSLLSWLRATEQMSGTKIFDPAKTPLLAAWMERFGELEAAKAVLQDVDRVVEYAMKKAHAAAAAAAADNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1J334</accession>
    <name>I1J334_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100832005</name>
        <name evidence="11" type="ORF">BRADI_5g25340v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580767.1">
        <property type="nucleotide sequence ID" value="XM_003580719.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J334"/>
    <dbReference type="FunCoup" id="I1J334">
        <property type="interactions" value="1788"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J334"/>
    <dbReference type="EnsemblPlants" id="KQJ85169">
        <property type="protein sequence ID" value="KQJ85169"/>
        <property type="gene ID" value="BRADI_5g25340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832005"/>
    <dbReference type="Gramene" id="KQJ85169">
        <property type="protein sequence ID" value="KQJ85169"/>
        <property type="gene ID" value="BRADI_5g25340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832005"/>
    <dbReference type="eggNOG" id="KOG1677">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045191_0_0_1"/>
    <dbReference type="OMA" id="HGANSHP"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
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        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
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            <begin position="34"/>
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        </location>
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        </location>
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        </location>
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            <begin position="28"/>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ85169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="298" mass="31230" checksum="0EBD97CCFB32C266" modified="2012-06-13" version="1">MDGAAAARKRSRTDTANGGAKRSKETESQQTGLSSKSKPCTKFFSTVGCPFGEGCHFLHFVPGGYQAVSKTHNLGHAAASAPSRAPVDHGANSHPAPAGKTRMCTKYNTAEGCRFGDKCHFAHGERELGRTTYESPYAPPMGGRFGSRHEPHPAMMGPTTGSFGASATAKISVDAALAGGIIGKSGVNTKQICRVTGVKLSIRDHESDPNLKNIELEGNFDQIKQASDMVGELIATISASTPLKNPAGAVPPAGRGGPGGRSNYKTKICENFLKGTCTFGDRCHFAHGENEQRKGAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E1D3</accession>
    <name>A0A0Q3E1D3_BRADI</name>
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        <submittedName>
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        </submittedName>
    </protein>
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        </location>
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            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ81502.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="186" mass="19936" checksum="9EE2F002C069C7DB" modified="2018-03-28" version="2">MGFLGPPWASQVPTRSPRAAMSEPASGSSCGGGDGGGDDIKLATGKKPAMKEHRRSETASGSSGDATIKSQKVAEGDKAEAGKKAKMWTVTREYIDRLRKEKDAGPQIRSFDYLNDGTDPGADALRAIVAGAAAATAETEAEKARILEDYDTRGYAQVEVVEDPWSDKEMVRKLMAWKEKVQQLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E9W1</accession>
    <name>A0A0Q3E9W1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">BRADI_5g22083v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ84655">
        <property type="protein sequence ID" value="KQJ84655"/>
        <property type="gene ID" value="BRADI_5g22083v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ84655">
        <property type="protein sequence ID" value="KQJ84655"/>
        <property type="gene ID" value="BRADI_5g22083v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E9W1"/>
    <dbReference type="OrthoDB" id="686392at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ84655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="75" mass="8185" checksum="F4D1B85D0B01923C" modified="2016-01-20" version="1" fragment="single">WWLEAIQSSPSSARKGTTSLVMLIAWSIWKHRNTAVFDNSPPSIASLLDAVKAEARQWSRAGAVGLRALLPSDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EW45</accession>
    <name>A0A0Q3EW45_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100841674</name>
        <name evidence="3" type="ORF">BRADI_4g39410v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_014902021.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EW45"/>
    <dbReference type="EnsemblPlants" id="KQJ91727">
        <property type="protein sequence ID" value="KQJ91727"/>
        <property type="gene ID" value="BRADI_4g39410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841674"/>
    <dbReference type="Gramene" id="KQJ91727">
        <property type="protein sequence ID" value="KQJ91727"/>
        <property type="gene ID" value="BRADI_4g39410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841674"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EW45">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF631">
        <property type="entry name" value="PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="156"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ91727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="436" mass="47053" checksum="9DD10A5E71C61DF9" modified="2016-01-20" version="1">MPSVTVKLYSLIFKLLLRRRLSSLSVSDPAPAGAASSFGVSSRPADHHPSPHSNPAFSTAAPDAVATKDLHPDPLSSLHLRLFLPNPHHATPLNNPPPPPLRRNSFPPPASDVDGQHLLSRRASASFHGVSSSASSQPHYGGYLPTARSARKLPVIVQFHGGAFVTGAADSAANDAFCRRIARHCDAIVVAVGYRLAPENRYPAAFEDGVTVLRWIAKQANLAACGRMMAKGAGTCGTDSFGAAMVEPWLAAHADPSRCVLLGVSCGANIADYVARKAVEAGKFLDPVKVVAQVLMYPFFMGSSPTQSELKLANSYFYDKSTCLLAWKLFLPEDEFCLDHPAANPLLPGRGPPLKLMPPTLTIVAELDWMKDRAIAYSEELRKVNVDAPVLEYKDAVHEFATLDGLLKTPEAQACAEDIAIWVKKYISLRGHEFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F068</accession>
    <name>A0A0Q3F068_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_3g02391v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F068"/>
    <dbReference type="FunCoup" id="A0A0Q3F068">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93038">
        <property type="protein sequence ID" value="KQJ93038"/>
        <property type="gene ID" value="BRADI_3g02391v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93038">
        <property type="protein sequence ID" value="KQJ93038"/>
        <property type="gene ID" value="BRADI_3g02391v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3F068"/>
    <dbReference type="OrthoDB" id="606501at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Trypsin Inhibitor V, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prot_inh_pot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036354">
        <property type="entry name" value="Prot_inh_pot1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091:SF12">
        <property type="entry name" value="OS02G0124200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091">
        <property type="entry name" value="PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00280">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CI-2 family of serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00285">
        <property type="entry name" value="POTATO_INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ93038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="65" mass="7206" checksum="257EBC19849B79B1" modified="2016-01-20" version="1">MAKTSWPEVVGWVSLNAWYQIHGDRPDVSIGFYVQGSPLPPGYDPKRVILILNDSQVVIQTPVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FBC6</accession>
    <name>A0A0Q3FBC6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g28586v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FBC6"/>
    <dbReference type="EnsemblPlants" id="KQJ97063">
        <property type="protein sequence ID" value="KQJ97063"/>
        <property type="gene ID" value="BRADI_3g28586v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97063">
        <property type="protein sequence ID" value="KQJ97063"/>
        <property type="gene ID" value="BRADI_3g28586v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FBC6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ97063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="167" mass="18540" checksum="503DFD327F7AC8CE" modified="2016-01-20" version="1">MAHPPRRLGRCARCVELDPARFCDPAMPRSLVRLCGSGNEEGPSSSLLSPPQSASLLHSTSPSFLLPEQQHHLRPPFLLQALSLNHLFVHLQLRDTRRRRQDCIAVHGLVAAGSGLPLPRCRSGHPLPRCGRIQPHCSLSTFPLSWIHSAPLTTRRIRARPLRRSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FR31</accession>
    <name>A0A0Q3FR31_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_3g50263v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FR31"/>
    <dbReference type="FunCoup" id="A0A0Q3FR31">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FR31"/>
    <dbReference type="EnsemblPlants" id="KQK00557">
        <property type="protein sequence ID" value="KQK00557"/>
        <property type="gene ID" value="BRADI_3g50263v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00557">
        <property type="protein sequence ID" value="KQK00557"/>
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    </dbReference>
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        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="OPT"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
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            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="329"/>
            <end position="350"/>
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    </feature>
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            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="425"/>
            <end position="442"/>
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    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="517"/>
            <end position="538"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="576"/>
            <end position="594"/>
        </location>
    </feature>
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            <begin position="606"/>
            <end position="631"/>
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            <end position="668"/>
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        <location>
            <begin position="1"/>
            <end position="45"/>
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            <end position="33"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="682" mass="74868" checksum="7A1888C98E236498" modified="2016-01-20" version="1">MDVIVPDRSQRVMPEAEIEKQENDEAAEGDMESDPARGGQLQAPPPERWQEELTVRGLVAALLIGFIYTVIVMKILLTTGQVPTLNVSAALIAFLSLQGWTRLLDRLGVPCRPFTRQENTVVQTCVVACYTIAFGGGFGSTLLGLNKRTYEQTGDSAGNVPGSWKEPGIGWMTGFLLAVSFGGLLTLIPLRKVLVIDYKLTYPSGTATAVLINGFHTTQGDKNSKKQIHGFLKYFGLSFFWSFFQWFYTGGDVCGFVQFPTLGLKAWKQTFFFDFSATYIGAGMICPHIVNISTLFGAIISWGLMWPLLSKRKGDWYPANVPESSMKSLYGYKAFICIALIMGDGVYHFTKIIGITSKSMYRQFNRKRVDNRVRNVDNTIALDELQRVEVFKKGHISSWMAYTGYALLSVVAVITMPIMFRQVKWYYVVIAYALAPVLGFANSYGTGLTDINMGYNYGKIALFVFAGWAGKDNGVVAGLVGGTLVKQLVLISADLMHDLKTSYLTLTSPRSMLVGQAIGTAMGCIVSPLTFMLFYKAFDIGNPDGYWKAPYALIYRNMALLGVEGFSVLPKYCLELSGGFFAFAALSSIARDVLPHKYGMYVPLPMAMAVPFLVGGSFAIDMCIGSLVVFIKEKLNKKEAGFMVPAIASGLICGDGIWTFPSSLLALAKIKPPICMKFTPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GMH6</accession>
    <name>A0A0Q3GMH6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100831635</name>
        <name evidence="4" type="ORF">BRADI_2g61490v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003567459.4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GMH6"/>
    <dbReference type="EnsemblPlants" id="KQK11661">
        <property type="protein sequence ID" value="KQK11661"/>
        <property type="gene ID" value="BRADI_2g61490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831635"/>
    <dbReference type="Gramene" id="KQK11661">
        <property type="protein sequence ID" value="KQK11661"/>
        <property type="gene ID" value="BRADI_2g61490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831635"/>
    <dbReference type="OrthoDB" id="1937632at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OS01G0962100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="26"/>
            <end position="49"/>
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            <end position="279"/>
        </location>
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            <begin position="137"/>
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            <end position="134"/>
        </location>
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            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK11661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="307" mass="33152" checksum="34BAD996B389CA32" modified="2016-01-20" version="1">MYSSSSSSSCLVVALARWFLRLAVQLPALICLEAMACALAFLTSPLRVLAAADRERKLDGLLGEMEAQMEKVVWENRALEEKLKAALMEQADLEDVLDEMDQEHDEAFARIRFLEAQMKALKKENLRLRELKGKAAWDEKKKPAPALTAGGREEEQEEGEEEQEEGFVFVSGDDSSKAAAEEEAQRQLGLLAATARRRSLFSVGMSAAVGGVAAWAAAEDAPAHACLPLLAGLLAVVAMSMCSVARLFRARHGRRQASGAVALLSVNWFLLGVLTYPMLPGVARAVLPRAARLAGPAIAWLAAAVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GTG3</accession>
    <name>A0A0Q3GTG3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="19">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100839254</name>
        <name evidence="22" type="ORF">BRADI_1g14630v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="18">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell, into the endoplasmic reticulum, or into organelles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Membrane</location>
            <topology evidence="1 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="19"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232633.1">
        <property type="nucleotide sequence ID" value="XM_010234331.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GTG3"/>
    <dbReference type="FunCoup" id="A0A0Q3GTG3">
        <property type="interactions" value="1802"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GTG3"/>
    <dbReference type="EnsemblPlants" id="KQK14183">
        <property type="protein sequence ID" value="KQK14183"/>
        <property type="gene ID" value="BRADI_1g14630v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74439">
        <property type="protein sequence ID" value="PNT74439"/>
        <property type="gene ID" value="BRADI_1g14630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839254"/>
    <dbReference type="Gramene" id="KQK14183">
        <property type="protein sequence ID" value="KQK14183"/>
        <property type="gene ID" value="BRADI_1g14630v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74439">
        <property type="protein sequence ID" value="PNT74439"/>
        <property type="gene ID" value="BRADI_1g14630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839254"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GTG3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Calcium-transporting ATPase"/>
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    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000011">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF420">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE 3, PLASMA MEMBRANE-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 19" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="15 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 19" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5006203237" description="Calcium-transporting ATPase" evidence="20">
        <location>
            <begin position="21"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="428"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="991"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1023"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="147"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006124"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361146"/>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="EMBL" id="KQK14183.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK14183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1058" mass="114621" checksum="6A12594EC1D1FEBF" modified="2016-01-20" version="1">MHNGVLCVCLFARPLQLAFSLPGTASSPAPQALPCRRIPPNPWARPGFGATTVTLRRSCRRKPCCGVESSDRRFPFAAAGELSNSNRAQHEKPRVSVFASKGAVQLQNGISLQNEYVVPEDVKAAGFRIDPDELTSIVESRDTKRLTAQGQSAGIADKLATSLTDGISTDEDLLNRRQEIYGVNKFAESEVRGLWEFVSEALQDTTLIILTACAFVSLAVGTATEGWPNGSHDGIGIVASILLVISVSATSDYQQSLQFRDLDREKRKILVQVTRNEFRQRMLIDDLLPGDVVHLAVGDQVPADGLFISGFSVLVDESSLTGESEPVDVNEGKPFLLSGTKVLDGSCQMLVTAVGMRTQWGKLMAALTEGGNDETPLQVKLNGVANIIGKIGLFFAVLTFIVLSQGLIGQKYHEGLLLSWSGDDVLEILNHFAVAVTIVVVAVPEGLPLAVTLSLAYAMKKMMNDKALVRQLAACETMGSSTVICSDKTGTLTTNRMTVVKACICGNTVEVNDLLIPSSLSPKIPGIAAQTLLESIFNNTGGEVVINQDGKPDILGTPTEAALLEFALSLDGKYKQKRQETKIVKVEPFNSTKKRMSVILELPGGGYRAHCKGASEIVLAACDKFIDDRGSIVPLDRKTADKFNGIIETFSSEALRTLCLAYKALEHGFNHEEIPLQGYTFIGIVGIKDPVRPGVRESVASCRSAGIAVKMVTGDNINTARAIARECGILTDGLAIEGAEFREKTPKELLELIPKIQVLARSSPLDKHTLVKHLRTTFNEVVAVTGDGTNDAPALREADIGLAMGIAGTEVAKESADVVILDDNFSTIVTVAKWGRSVYVNIQKFVQFQLTVNVVALLVNFSSACFTGDAPLTAVQLLWVNMIMDTLGALALATEPPNDNLMKKAPVGRTGKFITNVMWRNILGQSLYQFTVIWYLQTQGRYIFGLEGSQSDIVVNTIIFNTFVFCQVFNEVSSREMEEVNVLKGLSENSIFIGVLTGTIIFQFILVQFLGDFANTTPLTQQQWLLCVLFGFLGMPIAAAIKLIPVQPRKEDDKHTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GU59</accession>
    <name>A0A0Q3GU59_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">COBRA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_1g16791v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COBRA family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14512.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GU59"/>
    <dbReference type="STRING" id="15368.A0A0Q3GU59"/>
    <dbReference type="EnsemblPlants" id="KQK14512">
        <property type="protein sequence ID" value="KQK14512"/>
        <property type="gene ID" value="BRADI_1g16791v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14512">
        <property type="protein sequence ID" value="KQK14512"/>
        <property type="gene ID" value="BRADI_1g16791v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GU59"/>
    <dbReference type="OrthoDB" id="2014623at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010215">
        <property type="term" value="P:cellulose microfibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056900">
        <property type="entry name" value="COB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006918">
        <property type="entry name" value="COBRA_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052">
        <property type="entry name" value="COBRA-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052:SF34">
        <property type="entry name" value="OS07G0690900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25079">
        <property type="entry name" value="COB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04833">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999590" description="COBRA C-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="COBRA C-terminal" evidence="8">
        <location>
            <begin position="444"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK14512.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="685" mass="72058" checksum="B3F80068B82132A6" modified="2018-03-28" version="2">MASTIVSSLAVFFFLLLLLALAVAQPAVTPPPLPDDTAGCNGVLLTYTLQGRDRIRPFVSPADAQSQPYSFRATATVLNSGVRPLAAGSWSLLLAFAHREVLVSVSGAVLTSGADLPFNTTTAEAGALTSFSESPQRDLLTPIATAGDLARIQATVTLVGTHFAGPEPLTPPPLPSNISLLAGGGYTCSPPAVNNASLSTCCVPGNDPMAAPPLAPPTSTLSRGVSGDLVITYDVLQAHESTYLALVTLDNDAPMGRLDGWRLSWEWARGEFVGAMRGAHPLELDAGACVYGAQGEHYKDLDFFSGAVLTCARRPVILDLPPTRRDDAAMGNIEHCCRNGTLLPKSMDDAPGSSSSSSRSAFQMEVYKMPPDLNRTSRPHPPASFRISGSSPLNPDYTCGQPVTVRPSEFPDPSGLESTTRAIATWQASCNITVDNGSGGKPPQCCVSFSAFYNDSVIPCNTCACGCPAPASSASCSRTAPATLLPPHALLMPADRRAKEALKWANDEDLPLPANPPPCGDMCGVSVNWHVATDAEGGWSARVTLFNWGPGADMPDWFAAVVMDDKVYAGFEQAYSFNGTAVGNGTVFLRGREGFNELLLRESNMSGVDYPVPGKLQSVLSFTKKIDGGGDIDVVAGDGFPSKVFFNGEECAMPQRIPSKGVGVRAGISGNALLLLLLSKHLIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H1U6</accession>
    <name>A0A0Q3H1U6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100825875</name>
        <name evidence="13" type="ORF">BRADI_1g31333v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">May be a cell surface adhesion protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fasciclin-like AGP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16903.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560388.1">
        <property type="nucleotide sequence ID" value="XM_003560340.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H1U6"/>
    <dbReference type="FunCoup" id="A0A0Q3H1U6">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3H1U6"/>
    <dbReference type="EnsemblPlants" id="KQK16903">
        <property type="protein sequence ID" value="KQK16903"/>
        <property type="gene ID" value="BRADI_1g31333v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825875"/>
    <dbReference type="Gramene" id="KQK16903">
        <property type="protein sequence ID" value="KQK16903"/>
        <property type="gene ID" value="BRADI_1g31333v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825875"/>
    <dbReference type="OrthoDB" id="694090at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000015">
        <property type="entry name" value="Fasciclin-like arabinogalactan protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033254">
        <property type="entry name" value="Plant_FLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32382">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32382:SF6">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129208" description="FAS1 domain-containing protein" evidence="11">
        <location>
            <begin position="23"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="12">
        <location>
            <begin position="43"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK16903.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="289" mass="29823" checksum="E80E4AC5669AAC11" modified="2018-03-28" version="2">MAPPKLSLLALLFLLFPAAGNAAAAAAAADPADTVAAAAPPALFNVTEILGRFPDFTLFNYLLTKTKVSAQINRRGTVTILAPVNADVDWLLRRSSRLPRAALVELLAVHVILDYIDAAKLAALPRGRPGAPPVVATTLYQTMGVAPAGDRSGFLTITPTGNGGAVFASAAPGALVNAPFKKAVTAKPYNISVLQISNFVVPPGVISRPRFGPPPPRRIRSMAVAPSPAPAPVLPAVPPVTMPVEEGTTETIPVAAPEPSDGNRAVEAMRLWFGAGVVMALACVLVTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H247</accession>
    <name>A0A0Q3H247_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g06833v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H247"/>
    <dbReference type="EnsemblPlants" id="KQJ82155">
        <property type="protein sequence ID" value="KQJ82155"/>
        <property type="gene ID" value="BRADI_5g06833v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82155">
        <property type="protein sequence ID" value="KQJ82155"/>
        <property type="gene ID" value="BRADI_5g06833v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H247"/>
    <dbReference type="OrthoDB" id="690705at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33026:SF7">
        <property type="entry name" value="OS03G0100275 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33026">
        <property type="entry name" value="OS06G0360600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ82155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="239" mass="27416" checksum="110AFDB4954172D7" modified="2016-01-20" version="1">MEKIPRSLLGERVIFCDYVLRGLSFRLHAFFRAMLVKPEVRYFNLQLRESVQHWRSKWFYVRDDSVGKANKQLPAFTVKGSVKKIVAWKHESTASEREEVDSLMSRIYALMDQGLTGIHLIALFLKMRIQPLQAHAHPMWSYMGPLDPTRVNAEELTPEEVEERVARLTSSSVSSSVSLDSLVVPYGLARPMEEGLEDPFRQPPLTVQTESSDEVLESPTRVADPEVEEVSTETQRKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H386</accession>
    <name>A0A0Q3H386_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g14061v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblPlants" id="KQJ87882">
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        <property type="gene ID" value="BRADI_4g14061v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ87882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="93" mass="10332" checksum="83A395D583DC1004" modified="2016-01-20" version="1">MENDVGVVGDGRAVVTWTSAMSTFMLKHMAALVESGARTSSGFKMMQYNGCARVLNEHFHQVLTGTQMSNHYMTIKKKFQKIKKIKDDCSGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H636</accession>
    <name>A0A0Q3H636_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g22041v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodium</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H636"/>
    <dbReference type="EnsemblPlants" id="KQJ88914">
        <property type="protein sequence ID" value="KQJ88914"/>
        <property type="gene ID" value="BRADI_4g22041v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88914">
        <property type="protein sequence ID" value="KQJ88914"/>
        <property type="gene ID" value="BRADI_4g22041v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H636"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ88914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="200" mass="21648" checksum="1FB6BB9201B98B5C" modified="2016-01-20" version="1">MEAESEILESQGARHASIPVRLPSHLNSASPSLKRTPGAAASQSLTPKILLSVLLPSFVYLNSERERPAAMAGGGVSKELGHTLLQAKCASKTIRHNRDLLLQFQIHLQKDNDERRRRRGGERGIDVDELVYVTGIEAGARYLGSGQRSSALLLAGHGVPCPLLSAVHSNPTAAGLRSPRTSARKVADLEKKLWLCSGWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H7I5</accession>
    <name>A0A0Q3H7I5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g45858v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H7I5"/>
    <dbReference type="EnsemblPlants" id="KQK19000">
        <property type="protein sequence ID" value="KQK19000"/>
        <property type="gene ID" value="BRADI_1g45858v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19000">
        <property type="protein sequence ID" value="KQK19000"/>
        <property type="gene ID" value="BRADI_1g45858v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H7I5"/>
    <dbReference type="OrthoDB" id="440676at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK19000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="60" mass="6860" checksum="9B144FD9956703C4" modified="2016-01-20" version="1">MSIKLSKALNFEGIRSMVERVSQKNSTQWDAKDVESTANRSMQEVGKNDENIVTVETEWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HD62</accession>
    <name>A0A0Q3HD62_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">X8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100823015</name>
        <name evidence="10" type="ORF">BRADI_4g03190v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003579178.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HD62"/>
    <dbReference type="STRING" id="15368.A0A0Q3HD62"/>
    <dbReference type="EnsemblPlants" id="KQJ86083">
        <property type="protein sequence ID" value="KQJ86083"/>
        <property type="gene ID" value="BRADI_4g03190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823015"/>
    <dbReference type="Gramene" id="KQJ86083">
        <property type="protein sequence ID" value="KQJ86083"/>
        <property type="gene ID" value="BRADI_4g03190v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823015"/>
    <dbReference type="OrthoDB" id="417697at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044788">
        <property type="entry name" value="X8_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044">
        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF52">
        <property type="entry name" value="OS01G0631500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129244" description="X8 domain-containing protein" evidence="8">
        <location>
            <begin position="27"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="9">
        <location>
            <begin position="36"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ86083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="173" mass="18547" checksum="A8C8FB431C171242" modified="2016-01-20" version="1">MASPTATTLCFLVVGVLALLASPALAEFDCEPKGPSFCAARSTVGDDRLQAALDYACGHGADCSAIQPGAPCFDPNTKTAHASYAFNDYYQRHGRTPSACDFAGAGFIVHTGPEPDICEHIRFKSLICMRWACKLECVDNNPTKIIEGAYCTHKKGLRYCNCKVCVKAPPAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HN65</accession>
    <name>A0A0Q3HN65_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_1g78335v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PMEI family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HN65"/>
    <dbReference type="EnsemblPlants" id="KQK24133">
        <property type="protein sequence ID" value="KQK24133"/>
        <property type="gene ID" value="BRADI_1g78335v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK24133">
        <property type="protein sequence ID" value="KQK24133"/>
        <property type="gene ID" value="BRADI_1g78335v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HN65"/>
    <dbReference type="OrthoDB" id="679957at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009827">
        <property type="term" value="P:plant-type cell wall modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35357:SF8">
        <property type="entry name" value="OS01G0111000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35357">
        <property type="entry name" value="OS02G0537100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033724949" description="Pectinesterase inhibitor domain-containing protein" evidence="4">
        <location>
            <begin position="17"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="5">
        <location>
            <begin position="17"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK24133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="279" mass="29448" checksum="6C99DE95B83B6B1D" modified="2016-01-20" version="1">MRATAVVVLFLAGADSIMNATCSSLGAYPGYSYCVNVLSSDPSSGNAKDARDLAIIAANATSHNITQTAHTIDELLVYLQRCSADYSHYMGDLVDGRDPTNKLFYASIVGPDSCNVEFDQNQEWGFTKELLTDEDAQNAALTIFTANVALLNSLNKSLALPPSPSPASIINATCYALPDLYEEFERTLDLAVIAIHAVQRNITSAVRLIDGLVSDLAGCKAAYGAMAQKVGGALEDLVARRDHVGAANKLADASHDPVSCDAGRAHFASNIASSLGQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IES1</accession>
    <name>A0A0Q3IES1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Auxin efflux carrier component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100841055</name>
        <name evidence="9" type="ORF">BRADI_3g41080v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the auxin efflux carrier (TC 2.A.69.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574813.1">
        <property type="nucleotide sequence ID" value="XM_003574765.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IES1"/>
    <dbReference type="FunCoup" id="A0A0Q3IES1">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99099">
        <property type="protein sequence ID" value="KQJ99099"/>
        <property type="gene ID" value="BRADI_3g41080v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841055"/>
    <dbReference type="Gramene" id="KQJ99099">
        <property type="protein sequence ID" value="KQJ99099"/>
        <property type="gene ID" value="BRADI_3g41080v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841055"/>
    <dbReference type="OrthoDB" id="2133778at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010329">
        <property type="term" value="F:auxin efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010315">
        <property type="term" value="P:auxin export across the plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009926">
        <property type="term" value="P:auxin polar transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051107">
        <property type="entry name" value="Auxin_Efflux_Carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31752">
        <property type="entry name" value="AUXIN EFFLUX CARRIER COMPONENT 1B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31752:SF11">
        <property type="entry name" value="AUXIN EFFLUX CARRIER COMPONENT 5C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ99099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="39427" checksum="15ADB7B418D4353F" modified="2016-01-20" version="1">MIAWGDIRKVVSAMAPLYFALGLGYGSSRWWHLLASPEQCAAVNTLVAFFSMPFFTFDFVSGVDPSAVNFRVLAADALAKLLAILAAWAWAWARQYDDWSWPITGFSLATLSNTLVVGVPLLEAMYGDWARELMVQVAVAQSVVWVPLLLLAFELRNACCVLHLQPEPETKTKDGGGDVELAAAAAARVERDPNARAGNGNGNKKIRRCWAMARTVGIKVASNPNVYASVLGVAWACAAYRWRVGMPGVVTGALQVMSRTGTGMSMFSMGLSMAQQERMVACGAGLAALGMALRFVAGPAAALAGAAAMGIRGNVLRFVVVQAALPQSIASFVFAKEYGLHADVLSTAVIFGTLLSLPVLIAYYALLDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IUV2</accession>
    <name>A0A0Q3IUV2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g27801v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89776.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
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    </evidence>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Bevan M.W."/>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
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                <person name="Grimwood J."/>
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                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
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                <person name="Vogel J.P."/>
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                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Bd21</strain>
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    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="GeneID" id="100820846"/>
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    </dbReference>
    <dbReference type="KEGG" id="bdi:100820846"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KPT4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="entry name" value="DENTIN SIALOPHOSPHOPROTEIN-LIKE PROTEIN"/>
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        <location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EMBL" id="KQK13121.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK13121"/>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="1023" mass="112018" checksum="3B6445A3DF10F2E3" modified="2016-01-20" version="1">MQSQSSSRVKMLAKVDGSGAIDYSVDPSTFGFPQDANQEFFNRALQYDYSVSRQGFEDKGFLPSLPDHVVNSTSSALGEYQQLDDFLRHLEPIESDGQRVQNNNGNIAHFSRASNTSCLDHVEDITSYDVDCRDDRAISFGSSCSTGIASYPYINLLQSNNCIADTEDGTWAALMQMQEALEASNEECSDLTFNNTELSGGSTMQHQVVWDNGCLTSPSFTSNFLPFPGEAEATVTNTRNICHLQNLVDMPHDNEQDISSFELKVPEPKGTTTSNVCERRDEMHSAQWGAYPGHNESSVLMPVAQDRQNSISHQQLSVSVNGVDGSVDNDMKKLHGIYECEEQMEIDSLLNSFGASSDTFSQAYEIFQKGETLSDVDKKVKLEESSSATCVSDTVSYTTQAGAAESALSNGSSCAQQYQSTSQSCGLFSSESQWKTMAGSAFPLWGCQNGVNEYNSLHTLETNHKDHLLYSGHTSVQQQQSESKTTKLELIDNAGSPYQEFTTGVDGPFCAKRGTVSVWAAHPSIMENCSSRVHSTHTEPSDMQLPVTPTAHIQVQLPAPSLSMESNSSLIGGTELKKVNQCHPDVQLPMTQTSHVQLPAQSFSKDPNSSSIEITELKKVEQHHPDTQLPITQISHVQLPSPSSSKDPNSALTGRTGLKKFQHHSDMQFSMTQTNHVQLPVPSLSKDPNSALIEGTELKKVEQHHLDMQLPMTHTSHAQLPAPSLSKDPNPAFIGGTKLKNVEQLDNYSHVGSDQQIILLSASKPSCSSGSPIKKFDDMVDSRPKKRKRPAANLLVQHAQVMSGCGSKPCKRTPELDLARATRTSVKKVDGENATMQHSTFISRAQERLVLTTSLIQHVLPVLPARLLASNVTNPSETIVYHISKLAVSDILDPVLSFGSDPNNFTRSEKMPPSETSTSGKEGSKILSEVLATFVTRYGKLESSLSRVEKALTFQDFAFELREIDRWSILNHLVKFGEYKTLQAGGCSNPGPDPSSTIIRKHVRVAATAPLCLPDGVRCRVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3L692</accession>
    <name>A0A0Q3L692_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BRADI_4g16068v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3L692"/>
    <dbReference type="EnsemblPlants" id="KQJ88157">
        <property type="protein sequence ID" value="KQJ88157"/>
        <property type="gene ID" value="BRADI_4g16068v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88157">
        <property type="protein sequence ID" value="KQJ88157"/>
        <property type="gene ID" value="BRADI_4g16068v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3L692"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3L692">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000432">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000288">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase EFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000627">
        <property type="entry name" value="Probable leucine-rich repeat receptor-like protein kinase At2g33170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051809">
        <property type="entry name" value="Plant_receptor-like_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008">
        <property type="entry name" value="OS04G0122200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27008:SF588">
        <property type="entry name" value="RECEPTOR KINASE-LIKE PROTEIN XA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033725089" description="non-specific serine/threonine protein kinase" evidence="17">
        <location>
            <begin position="22"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="625"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="658"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQJ88157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="909" mass="98303" checksum="9CD97B9546464967" modified="2016-01-20" version="1">MAGALALVVFSLLLCAFPSHALHPPSSNRMVSSNTTTADELSLLSFKSMLSGGPLPSWNSSGSYCSWPGVICGGRRHPDRVVALRLHSYNLSGWLSPSLGNLSFLQKLDLSDNQLVGQIPPELGRLIRLRLLNLSDNSLQGSIPAALRGCTKLTRLDLFSNQLQGEIPAKKIGALKVLSNLILKETIALYFLNLSGEIPPRLAELTSMEYMSLGHNRLSGEIPPGLGNLSKMWYLALSFNMLSGSIPSSFGMLSSLSTITLSSNNLTGAIPISFWNISSLKFLVEFITALTNCSQLQVLGLNANKFEGVLPISLSNLSTSLVHLELKSNRIKGSIPEGIGNLVNLQRLVLMQNSFTGTLPSSLGENKISGSIPWTIGNLTELNYLDLYMNDFSHDLPSTLVNLTKLFGLYVSSNNITGQIPSGLFDIGSIPKEIGNLKNLVDFRAESNKLSGEIPDTLGGCQLLQVLSLQNILNGGIPSVLGGLKGLETLDLSSNNLSGQIPKSLGDLTTLHYLNLSFNSLAGQVPIVGVFANSTAVSIQGNGKLCGGIPGLHLPLCSLQIAKKKHKFPVVPTLVSLISTLAVLALLYKLLTCQKKRKEMIPSTNSMQDRSLISYSHLVKATDDFSVSNLLGSGSFGSVYKGKLDGHAGESATFVAVKVLKLQTPKALESFTAECEALRNMRHRNLVKIITVCSSIDTQRNDFKAIVYDFMPNGNLEGWLHPHTNDKTEQKHLNLLQRVTILRDVAYALDYLHCHGPAPTVHCDLKSSNVLLDAEMYGAGNMVSTHGDIYSYGIVVLETVTGKMPTDSKSRQGLSLREYAQLGLCDRMLDVVDPRLSIDLGSGLKTANALSYRRALECLVSLLRLGMSCSQELPSSRMSTGDIIKELHAIGESLQGIQSMEMENCANKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LJR2</accession>
    <name>A0A0Q3LJR2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g00242v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92725.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LJR2"/>
    <dbReference type="EnsemblPlants" id="KQJ92725">
        <property type="protein sequence ID" value="KQJ92725"/>
        <property type="gene ID" value="BRADI_3g00242v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92725">
        <property type="protein sequence ID" value="KQJ92725"/>
        <property type="gene ID" value="BRADI_3g00242v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LJR2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ92725.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="9578" checksum="6C97407225569C00" modified="2018-03-28" version="1">MAEEEGKRKNRRELGTGAGLLGFGLGRKKGGNKGKKRKAGGPAREAQVTQRLYMAAPSPPSTRTEQEFICSVLVNCDCHQVACLLNAYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3P7B2</accession>
    <name>A0A0Q3P7B2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100846062</name>
        <name evidence="2" type="ORF">BRADI_5g23437v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580649.1">
        <property type="nucleotide sequence ID" value="XM_003580601.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3P7B2"/>
    <dbReference type="FunCoup" id="A0A0Q3P7B2">
        <property type="interactions" value="1433"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3P7B2"/>
    <dbReference type="EnsemblPlants" id="KQJ84873">
        <property type="protein sequence ID" value="KQJ84873"/>
        <property type="gene ID" value="BRADI_5g23437v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846062"/>
    <dbReference type="Gramene" id="KQJ84873">
        <property type="protein sequence ID" value="KQJ84873"/>
        <property type="gene ID" value="BRADI_5g23437v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846062"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3P7B2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000016">
        <property type="entry name" value="Transmembrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33471">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33471:SF7">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ84873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="328" mass="35229" checksum="FCAA0A5C1D5A1AD0" modified="2016-01-20" version="1">MAIVAALVAPTADPRNPLKLAAGPQHRHWRVAAASSSSSPAAAASGVDLQALRSAIDKKDSNEAKRTLDELKELGWAKRWSSQPYMSRRTTSLRELTTLGIKNAENLAIPSVRNDAAFLFTVVGTTGFLAVLAGQLPGDWGFFVPYLTGSISLIVLAVGSVSPGLLQVAIGSFSAVFPDYQERIARHEAAHFLVAYLTGLPILGYSLDIGKEHVNLVDEQLQKLIYSGQLDQKELDRLAVVSMAGLAAEGLEYDKVVGQSADLFTLQRFLNRTKPPLSKDQQQNLTRWAVLIAASLLKNNKAAHDALVSAMSEKASVLGCIEAIENAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QS22</accession>
    <name>A0A0Q3QS22_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dephospho-CoA kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_2g12800v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3QS22"/>
    <dbReference type="STRING" id="15368.A0A0Q3QS22"/>
    <dbReference type="EnsemblPlants" id="KQK04282">
        <property type="protein sequence ID" value="KQK04282"/>
        <property type="gene ID" value="BRADI_2g12800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04282">
        <property type="protein sequence ID" value="KQK04282"/>
        <property type="gene ID" value="BRADI_2g12800v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3QS22"/>
    <dbReference type="OrthoDB" id="247245at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3QS22">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00152">
        <property type="entry name" value="dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK04282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="18816" checksum="ED38E6370E5A1E52" modified="2016-01-20" version="1" fragment="single">GRLRKIVFSDPVELQLLNRLLAPHITCGIFWKISKLWLKGCKVIILDIPLLFEAKMDQWTDPVIVVWVDPKTQIERLVSRDGCSEEQAQNRIDTQLALDWKKSQADMVVNNSGSLDDTKEQLQEVLKQVSGDLTWKERLRSRDDLVSIVLCTTIGVLLAHKNLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CFB2</accession>
    <name>A0A2K2CFB2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g03615v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CFB2"/>
    <dbReference type="EnsemblPlants" id="PNT60712">
        <property type="protein sequence ID" value="PNT60712"/>
        <property type="gene ID" value="BRADI_5g03615v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60712">
        <property type="protein sequence ID" value="PNT60712"/>
        <property type="gene ID" value="BRADI_5g03615v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CFB2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT60712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="210" mass="23663" checksum="C29FA0C49B464607" modified="2018-03-28" version="1">MLYYLHFDRDPEIHAEDRRTVEGDAFVRVTIWVFPRREQPRGPHRSIAAGEDFEDASINAIHQMLHRLGGLYQDELHGTPIRHFAFRREGGPLQLSQYLRTETNPTVLHMTRYSYGLEGRLLTTSGMLRDMHARLQHNRRRMEILEERLVEANTALVAAQAAAHNAEAAAKEAINNTNDQAQAAIEANNTMAEVAVHAAQEQAQHAAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CJ77</accession>
    <name>A0A2K2CJ77_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BRADI_5g25175v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT62081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CJ77"/>
    <dbReference type="EnsemblPlants" id="PNT62081">
        <property type="protein sequence ID" value="PNT62081"/>
        <property type="gene ID" value="BRADI_5g25175v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62081">
        <property type="protein sequence ID" value="PNT62081"/>
        <property type="gene ID" value="BRADI_5g25175v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CJ77"/>
    <dbReference type="OrthoDB" id="674570at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CJ77">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000337">
        <property type="entry name" value="Wall-associated receptor kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF461">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036043234" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="31"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="301"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="409"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="PNT62081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="73098" checksum="EF673C75045AC14A" modified="2018-03-28" version="1">MATAPLQSRFQLLPIILLLLALATNHLVLAAEELQPKIARPGCRDKCGNISIPFPFGIGPNRCFCEKGFEVLCDDSASPPRAFLADNRTNQYVSQGSASVTDAGATSHEDNSTALPLELVSISVNTGEARAYASEYFIQQHAVRGVGDAERFDSSCTAYPPYYGYVAANGSCNGRGCCEGTLPLEIIPIKYVEVSLRSKTNHLSETNLCSYGMVVEKYIFSVPDMNGDKVLVKRHPRGVPFVLEFAIGNASCPAEGQRPPADYACVSAYSSCANATSGEGYVCKCWDNYDGNPYITDGCQDIDECKSPELYYCSSNGVCKNRPGGYDCPCKRGMRGDGKADTCKEIFPLVARVIVGAVGSVGFTIVTFLIILLKERRKMKEFYKKNGGPILEKAKLIKLFKKKELKQILKGSNIIGKGFFGEVYKGLLDNKMVAIKKPINAGVLENEQFANEVIIQSQVSHKNIVRLLGCCLEVDTPMLVDLSLDLRLSIAAQSADGLAYMHSKTNTQILHGDVKPANILLDDNFAPKIADFGLSRLIVRDTKHTEFVIGDMNYMDPIYQKEGLLTEKSDVYSFGVVILELISRRKAIHRDTNNLVNSFREVHEKERKPTELFDKDIAVTEDLEVLNSLTEMAMECLSLEVDQRPTMTEVAERLFMMSRSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CNB5</accession>
    <name>A0A2K2CNB5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF2470 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100835872</name>
        <name evidence="2" type="ORF">BRADI_4g16885v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CNB5"/>
    <dbReference type="STRING" id="15368.A0A2K2CNB5"/>
    <dbReference type="EnsemblPlants" id="PNT63509">
        <property type="protein sequence ID" value="PNT63509"/>
        <property type="gene ID" value="BRADI_4g16885v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63509">
        <property type="protein sequence ID" value="PNT63509"/>
        <property type="gene ID" value="BRADI_4g16885v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1873930at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CNB5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.10">
        <property type="entry name" value="PNP-oxidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037119">
        <property type="entry name" value="Haem_oxidase_HugZ-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343">
        <property type="entry name" value="CREG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343:SF28">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT (PPR) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="52327" checksum="92A12D6ACF03FBD5" modified="2018-03-28" version="1">MLLAAAAAAAPDHPFFFLSRRLPPPPLSFFAARRAAAGGGSAVSPRAQLRRCRSQTRSHHSSHCRSHSSPSSWCGNVHARRSRHCRVGATTPDELPPGRGGSYHPSEDIAETLQLNDGEPARLTDAESARTIVEVHNKATVMISTLIDDGVHERIILPEFPYLTDENGDIYFEVDNEDTVMESIMGDDKLAHVIIGLDNTEVFADLDLAASSAEFVQEDDEDDDDSDDEESDFEDDFEEEGVFDVDEEDGGDDDDDDEDAPSWSNLETLNSCHPLYFARMIVESANKSSIDWLDRPPASLVVEGQLRPAFAEESTMVSRHLLNGDEPLKDRKESGATFFKVEVLSIELITAYGTEPKVKIEEYRRSRPDIIAHSAPNIISRLRAGGDKITQALKSLCWRCKAIKVEEAAIIGVDCLGFDLRLCSGTQVQTLRFAFPTKSSAQRSKYMNFSFLGYIKKGSHHKPSRKSLDVIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CNH9</accession>
    <name>A0A2K2CNH9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g18232v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CNH9"/>
    <dbReference type="EnsemblPlants" id="PNT63601">
        <property type="protein sequence ID" value="PNT63601"/>
        <property type="gene ID" value="BRADI_4g18232v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63601">
        <property type="protein sequence ID" value="PNT63601"/>
        <property type="gene ID" value="BRADI_4g18232v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CNH9"/>
    <dbReference type="OrthoDB" id="692647at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT63601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18143" checksum="2E3F36CF9309FB05" modified="2018-03-28" version="1">MEMVGPLRKWIKCAGTSYGLVTSKRTEIDQLAMLWDRLLTVQLEGEPDLISWKGSADGEYSAKAAYKAHFLQAIPQPLLNSVWSIKASSFTMRSWWRCIVSGPKSETKKETTMAAIIVWHLWKERNNRVFQQKECNSISLYRLIEDEFCLLLEAIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQ69</accession>
    <name>A0A2K2CQ69_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g25476v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQ69"/>
    <dbReference type="EnsemblPlants" id="PNT64176">
        <property type="protein sequence ID" value="PNT64176"/>
        <property type="gene ID" value="BRADI_4g25476v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64176">
        <property type="protein sequence ID" value="PNT64176"/>
        <property type="gene ID" value="BRADI_4g25476v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQ69"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT64176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="221" mass="22174" checksum="A2A5BEC8EFF9B514" modified="2018-03-28" version="1">MADTPLRDTVPLEEGHPPFSFPPHPLTPLALTPPEFLAGITSAAGRSSLAVITSTSPSSPPRSADRVIFLASSRRPRRTLPRAQGAPEPPEPPSPEPPPPPERCGWRGGRGEAGPATGEGGVRRGRWGMGEAGAGGDGGRASGEMGGVGGGGSRRGGKRGRRGEGGESGASGAPRGGGEAGSGLGGGGYGRGARGGGGGGGEGWRRRSDARESGQRRLRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQV4</accession>
    <name>A0A2K2CQV4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g28222v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQV4"/>
    <dbReference type="EnsemblPlants" id="PNT64410">
        <property type="protein sequence ID" value="PNT64410"/>
        <property type="gene ID" value="BRADI_4g28222v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64410">
        <property type="protein sequence ID" value="PNT64410"/>
        <property type="gene ID" value="BRADI_4g28222v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQV4"/>
    <dbReference type="OrthoDB" id="694714at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33170">
        <property type="entry name" value="DUF4283 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33170:SF40">
        <property type="entry name" value="OS04G0557100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="478"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="706"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="953"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="709"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="738"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="748"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="781"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="959"/>
            <end position="971"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT64410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1005" mass="102063" checksum="0F20CD0558B9CE7C" modified="2018-03-28" version="1">MREEDRHRGREVFFSGCDLGRSAPPLSSLAASGVEHDIAGARGPHPAFSGSPPHRGAPMVGATGREEERCCFFFNKPAAYDGLAGGVLGRQSELSDEEEDVASPPAGGGCVKLLAGAGSPEEMAFDAMDAGRRLKGPRKAAVEALNGRKGGSAAVGDCVSPSSARSTLGLLDSFVSGRVSMDLASGDAASDGGHAASGGSLAAGDAPSGVAPAAATVPAASAGMIGQAAHGAPLGGAGTAAGTWSGGAAATGTCAPRGSAAFGGGTAGYHRGNGGGFRGGYGGGYGNNYGGYGPSYGGGYGNGYGGGYGGCGYGNGYGGGRNYRFASSHGRFPYQGRGGGQRWGRGFRGTNEQQGGVGQQGGGEMVQGAEMSSSAATQSVTVQASEVATVQVDGPVLAGQGVAAAGVAATAGKGAVVGAAAAGQAVVGVAAAQESAVGAAAAGQPSAAAGQGRVAAGSSAAAASNEVISVTNPLAKVRCFRCEQKGHYSSMCTAVLCDFREKADHVSAECELPRLPKLSVRSCGTVADDLFYFELPEEAVVVPKVESNRKGLIQVEGGSLSQERVVAEMQRLIPVPNFQWEVTAQGMSDFVVLFPTRGELQQLVRVGVIQVPNSELRFRVDEWTSSSTAAFEFQDTWVNIGNIPRDLFNYRAIWGLGTLLGTTLNDMPFSRRHGVARVRVSVTNISAIPPGTDLRHEGRGYRLTFLVEPSEDEVMNDDDDDDDDDGNDDNKDKSNPDNNDDMDDEILDDMKDLDNKKREEERQTGKRSKPSRELPDGSMGGSSSAPPVMRGSSSPTTGLIFRTGSATGVGFLSPFKMFPEPAVELPSPEERRAVSAGMGARRPSNGGTGGTTVGCCPSLFAGYDGGAGGTSVGCGLSAGSDGGAGGTIGCCAVKGRGLDGAGSVGRERGGVSAVRVTCSSLAFSVVCVAAFFAFGVFELEAQLAMVGLRGGGGDDETSGAAQRGAVPAVGARGRRREYPPPSRFSAVLLPKLTLMTDDQQSNATC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQZ8</accession>
    <name>A0A2K2CQZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g28733v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQZ8"/>
    <dbReference type="EnsemblPlants" id="PNT64450">
        <property type="protein sequence ID" value="PNT64450"/>
        <property type="gene ID" value="BRADI_4g28733v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64450">
        <property type="protein sequence ID" value="PNT64450"/>
        <property type="gene ID" value="BRADI_4g28733v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQZ8"/>
    <dbReference type="OrthoDB" id="662234at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33207">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT64450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="32805" checksum="30F840696E227C19" modified="2018-03-28" version="1" fragment="single">MAAIANLTGTSLRRAALTFSYRCLSRHLSHSPSPPLLGHFFHPCPPSTQEPDETPILPPSAPAFQPLTASCPRLSLAFVPDAPRFSLSDSHLGLLLLLPNNPLFHYPDFPCFVVCDPARRLHATVPPPPVAMLSGARFVGAALLSRAAHPSGLRFEAVCLTVDVGRPRAPRAWVASFDDGECCWRSLPLPREVIFEFDIDSHWLERCCVRAAGSLYWHICMSRHVLALDAATLEFSFLPVPTEMSPGVEWHVQKNRVGEMPQDGRLCIGSLEFLAGMSLPDGGSAGDGAGQAAHRRAARRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CSC3</accession>
    <name>A0A2K2CSC3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g35208v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CSC3"/>
    <dbReference type="EnsemblPlants" id="PNT64940">
        <property type="protein sequence ID" value="PNT64940"/>
        <property type="gene ID" value="BRADI_4g35208v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64940">
        <property type="protein sequence ID" value="PNT64940"/>
        <property type="gene ID" value="BRADI_4g35208v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CSC3"/>
    <dbReference type="OrthoDB" id="668516at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012871">
        <property type="entry name" value="DUF1668_ORYSA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07893">
        <property type="entry name" value="DUF1668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT64940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="221" mass="24965" checksum="D6BF460B27AFB452" modified="2018-03-28" version="1" fragment="single">GPKNFAALWAVGSTVYALDCGLDAQRHSCFETLSREPLPALHPDGSTFFLSLDKSPWHLFLRHQAPRVGAPRTQLDAAFRRRGPLHGRRGGQVDAWVGLCSRRKCRLVACQVLSPRPSDDDDWGRELEPAPFQDKEGRVDLRSKWEQRHLGASLTYLGKAEFCLQELLTQKGYDFSMAAGVKVPMLLHLVTFQGGVLRRRLARTYKLSRDSSEYTPIAFRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYG0</accession>
    <name>A0A2K2CYG0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">At1g61320/AtMIF1 LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g20141v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CYG0"/>
    <dbReference type="STRING" id="15368.A0A2K2CYG0"/>
    <dbReference type="EnsemblPlants" id="PNT67067">
        <property type="protein sequence ID" value="PNT67067"/>
        <property type="gene ID" value="BRADI_3g20141v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67067">
        <property type="protein sequence ID" value="PNT67067"/>
        <property type="gene ID" value="BRADI_3g20141v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CYG0"/>
    <dbReference type="OrthoDB" id="613853at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF64">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="At1g61320/AtMIF1 LRR" evidence="1">
        <location>
            <begin position="50"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="At1g61320/AtMIF1 LRR" evidence="1">
        <location>
            <begin position="148"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="426" mass="48470" checksum="2E7B89DD297428AE" modified="2018-03-28" version="1">MLPVSRTFLSSWRCFPNLTFNWKTIGFNLDEDTYETAKKFKEYMARIYHIFENHSGTGVKTLELNLRPWGNDFTASHLDIWLQTAVKSGILELVVNLPDDHSPKYNFRCLLLSCAASSIQTLSLASAFRPTLMIGCLRNLKSLYLRLEFEISDCNEITSSLKIPSYLQRLSILRVRRCERLEVIEIYAPKVTSFVFIGPPTKISIVDSSQLETVVMNGSTYSGMFRYAVTKLYSFASNLHTLVLFSYGEAINSTPGSAHKFLQLRHLKIYFDGRKFHSSFDFLSMVSFLEACPVLETFFLSAGSRFRGRQEPTLKDTDADSWHIKRIPGFRHDKLKKVSITGVQSTKSLIELACQILESCSSLRCLVLDTTSGYDDRSMCENLGWEDVMEALKGFKAIKRHLKGKVPSSVELTVLKPCDRCHMSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D3K1</accession>
    <name>A0A2K2D3K1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g46114v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D3K1"/>
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        <property type="protein sequence ID" value="PNT68845"/>
        <property type="gene ID" value="BRADI_3g46114v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT68845"/>
        <property type="gene ID" value="BRADI_3g46114v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D3K1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT68845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="126" mass="14714" checksum="9A0EA8521D5A5AB8" modified="2018-03-28" version="1" fragment="single">AIHSHHTARHARTRNREREKEREREIGARGDDRTTIRRVEAGGADGARNLRADQRLRRAPPQVHRRRLPFHQHAPVRPGLLQKQRDRRRQPARPAGHPQRLDREGMPRAHRPQRPPQQGPPPPQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D450</accession>
    <name>A0A2K2D450_BRADI</name>
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        <recommendedName>
            <fullName evidence="9">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="11" type="primary">LOC100826056</name>
        <name evidence="10" type="ORF">BRADI_3g48697v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
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            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575352.1">
        <property type="nucleotide sequence ID" value="XM_003575304.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D450"/>
    <dbReference type="STRING" id="15368.A0A2K2D450"/>
    <dbReference type="EnsemblPlants" id="PNT69056">
        <property type="protein sequence ID" value="PNT69056"/>
        <property type="gene ID" value="BRADI_3g48697v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826056"/>
    <dbReference type="Gramene" id="PNT69056">
        <property type="protein sequence ID" value="PNT69056"/>
        <property type="gene ID" value="BRADI_3g48697v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826056"/>
    <dbReference type="OrthoDB" id="207175at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000003">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor ANT"/>
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    </dbReference>
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        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF72">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR BBM"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="291"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="393"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D5V9</accession>
    <name>A0A2K2D5V9_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g59729v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="InParanoid" id="A0A2K2D5V9"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT69663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="95" mass="10736" checksum="17A88D68258EE84B" modified="2018-03-28" version="1">MSHGNRREREDDETAANTTDFSRSDAPLSIIYRHMRGPRNHISSAHVLPFLDQTLLAIIASASPAQQWILAARVRGQLEVTSNRWEKHASAHVHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D6R0</accession>
    <name>A0A2K2D6R0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g03925v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT69970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_2g03925v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_2g03925v3"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT69970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="74" mass="8591" checksum="D8B5DE6E90CFFE3E" modified="2018-03-28" version="1">MANLSFLLPLVNKRHCAHYPYLQIIVRISACCRQTNVCIRAKISKLARFNGGNQHRRCNPSVELKCMQWRGIFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D6R4</accession>
    <name>A0A2K2D6R4_BRADI</name>
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        <submittedName>
            <fullName evidence="12 13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_2g03900v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2D6R4"/>
    <dbReference type="EnsemblPlants" id="PNT69968">
        <property type="protein sequence ID" value="PNT69968"/>
        <property type="gene ID" value="BRADI_2g03900v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69968">
        <property type="protein sequence ID" value="PNT69968"/>
        <property type="gene ID" value="BRADI_2g03900v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="1394818at2759"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D6R4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061:SF44">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
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    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033311666" evidence="9">
        <location>
            <begin position="28"/>
            <end position="492"/>
        </location>
    </feature>
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        <location>
            <begin position="41"/>
            <end position="79"/>
        </location>
    </feature>
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            <begin position="371"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="492"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
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            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
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            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="492" mass="54490" checksum="72031F1402E46815" modified="2018-03-28" version="1" fragment="single">MNRSSKHHLYVLLRLLLLICSYSTIDAASVAKQNTTVLCLPDQASSLLQLKRSLIDFDENLASWRAGSDCCHWKGITCDMASGRVISLDLGGFHMQGRRLDPALFNLTSLRNLSLAFIDFRRAQLPLYGFERLTNMIHLNFSETLFLGQILIGIARLKNLVTLDFSRNYVGLYLQEPSFETFMANLSNLRELRLDQVDISDGGSTWSGVLANSAPQLHILSLYGRGISGPIHPSFSRLRLLREIDLEYNELTGKVPEFFAEFSSLSILKISGTFSEGQFPAKIFQMKSLRTLSLSSNPSLFVCLPDFPVGSNLETLSLDGSNLACGIPPAFINLKYLKMLSISTIGVSNKLSLIHKLPSLSTLWLYGQDLETPTLSWIGNLSTLTSLVLWGYDFSQLAPSWIGNLKSLTSLFIMYCNFSGPIPHQIGNLTNLAELKSLDCSYNEQPLPSWIGNLTELTALMLSGCNLSGPIPSTLGNLMQLRSLQIVDSNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DB37</accession>
    <name>A0A2K2DB37_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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            <end position="27"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT71497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="111" mass="12171" checksum="415DA14A595A8F83" modified="2018-03-28" version="1">MSQSSSRLACSRRRSSPPPTLTSFSPASLGEYPCAAPFFISSIALEPRHQAHHAARQQATEQPSSPACGPSGGPPAKNYMAKANFQFRQRNSYFIHTCWNSINCEETNVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
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    <gene>
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    <organism evidence="3">
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DD72"/>
    <dbReference type="EnsemblPlants" id="PNT72228">
        <property type="protein sequence ID" value="PNT72228"/>
        <property type="gene ID" value="BRADI_2g41652v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72228">
        <property type="protein sequence ID" value="PNT72228"/>
        <property type="gene ID" value="BRADI_2g41652v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DD72"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT72228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="79" mass="9023" checksum="A842DEFD2EBAE841" modified="2018-03-28" version="1">MRKLRSKAHQSCRPRQASPPPQRKLNKLVLLFALARQSTTDPVRVAAVLLLGVACLLGCFKASTYFFSHVFEVRLWPYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DH71</accession>
    <name>A0A2K2DH71_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g61235v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DH71"/>
    <dbReference type="EnsemblPlants" id="PNT73619">
        <property type="protein sequence ID" value="PNT73619"/>
        <property type="gene ID" value="BRADI_2g61235v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73619">
        <property type="protein sequence ID" value="PNT73619"/>
        <property type="gene ID" value="BRADI_2g61235v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DH71"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT73619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="231" mass="25406" checksum="DABFA6725905E846" modified="2018-03-28" version="1" fragment="single">SLPLPLLIPQADRPQPSALLPSPQAISPRSLLFSTARSRLLLPKSGQLPDPRAPPRPPPWPRGPAAPTAWPARARLLHLRPSAATLAPAVPQPPRPQGRAQEHRPTSRSRRRPRIEAPPTLAPPHRSPSAGRPHRRRQAPPVASPPHQRRRCPRGAGVHTCGISASQSAPLMSRRDDELRLMKPLCPRNMLKISQPILVRSKTTLNMPKTGLGMIYLVLKISEFKLNGFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQH5</accession>
    <name>A0A2K2DQH5_BRADI</name>
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        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g49250v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQH5"/>
    <dbReference type="FunCoup" id="A0A2K2DQH5">
        <property type="interactions" value="492"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT76535">
        <property type="protein sequence ID" value="PNT76535"/>
        <property type="gene ID" value="BRADI_1g49250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76535">
        <property type="protein sequence ID" value="PNT76535"/>
        <property type="gene ID" value="BRADI_1g49250v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DQH5"/>
    <dbReference type="OrthoDB" id="618980at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF24">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="22"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="382" mass="42605" checksum="7E968FEB5B09D76D" modified="2018-03-28" version="1" fragment="single">RSRDAVTGLAAFRSEALLVFPLSRGWCLVLDARDASCHLSHLATGATAALPKLTAVCRADRRGGIITHDQGRYYLHYTDKESGRGSRSSHLWGPGEKIRIGCPGFWVWGFSTYLAFTEYMRFAVHVPPAGADQGGMLIMMYHALLGPTGMVFCRPGDAAWTKVEKPRPEVGAFGFIDFAYHAGKVFVLESDGILTVFDATTLDVLCLVQPPPPAALALLPTFLFELEYQVHLVALPSKLVLVRVTVKWSRPVAFDLFELAAGGQCWRKVADAGDYELFLDGYHASFRENAHGISGTRIYYVYCYHLVSSTAACFYSMAGTPIYYAHDQRWVLNNAAYCYSMQDNKLECVYTWPPPLDQVNNYSYRTPVDNYVISTKPSWFVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQM9</accession>
    <name>A0A2K2DQM9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g49847v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQM9"/>
    <dbReference type="EnsemblPlants" id="PNT76580">
        <property type="protein sequence ID" value="PNT76580"/>
        <property type="gene ID" value="BRADI_1g49847v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76580">
        <property type="protein sequence ID" value="PNT76580"/>
        <property type="gene ID" value="BRADI_1g49847v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DQM9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT76580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="242" mass="26263" checksum="AAA3173B50865B26" modified="2018-03-28" version="1">MHMFNGGSAKPTEVEFLTTLPTAVAPRSPRSANSFSASSPVKEHGGRVDIDWMDMFSCGSAEPPEFEFLPSLPAVVAPRSTRPAESFCVSSHANPVKKHGGRVDIDWMDMFNCGSAESPEFEFLPSPPSAVAPRSARPAESFCVSSNANPVKKHKARVDIDWMDMFNGRSAEPPEVEFSSPLPTAVAPRSARLAESFCVSSHANPVKKHGARVDIDWMEMFIGGSTELPEVDFSSPARGRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DSC0</accession>
    <name>A0A2K2DSC0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BRADI_1g58762v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DSC0"/>
    <dbReference type="EnsemblPlants" id="PNT77175">
        <property type="protein sequence ID" value="PNT77175"/>
        <property type="gene ID" value="BRADI_1g58762v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77175">
        <property type="protein sequence ID" value="PNT77175"/>
        <property type="gene ID" value="BRADI_1g58762v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DSC0"/>
    <dbReference type="OrthoDB" id="10022113at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000891">
        <property type="entry name" value="Calcium binding EGF domain containing protein, expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000043">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF296">
        <property type="entry name" value="CALCIUM BINDING EGF DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="489"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="PNT77175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="611" mass="67618" checksum="4747E47F491D6F50" modified="2018-03-28" version="1">MNQIIVKFSKTILVKSGVDVYNMSWTPGNSFAVLESIMILSVVACDLDVYLVHRNGDRTLLCMVTCPSIHMAEQVYNQEPHGPGSCDLTRSTLSFVRTIDLQFVHRKASRIKTQSNLSILWDEININFETFLFWSIPGKTSCSSSMKDNNYSCISNHSQCMVPPYLPGYVCRCSRGFEGNPYLLDGCSPDIDNGYSPRPRKENCSGRCGTINVEFPFGLEEACSARKSFQLKCSSHSTKPPILRLNPAIEVTYINVSDGLLGIKYDSTLGGMFINSHVNSVGTPSESQEPKLYVDPLESASVRWAVANLTCQDARDNTSGYACVSTDSSCPGVISSIEGYVGYRCVCLPGFEGNPYIPDGCIDIDDCAQTPGLCKGICQNTIGNYSCTKCPDHTEYDITKMQCTPKAKQNLFLGIIIGLSTGFGLLLLSLSAVLLVRRWKRDAEKKLRRKYFRMNQGLLLEQLISSDENASEKTKIFSLEELSKATNNFDTARILGHGGHGTVYKGILSNQHVVAIKKSKFVRKGEISDFVNEVAILSQINHRNIVKLFGCCLETEVPLLVYDFISNGSLFDVLHPADSSNIVLACELHQKLQVLCIISTLRHQYQSSIGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DUA7</accession>
    <name>A0A2K2DUA7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable glucan 1,3-alpha-glucosidase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glucosidase II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100830771</name>
        <name evidence="16" type="ORF">BRADI_1g69780v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; N-glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DUA7"/>
    <dbReference type="FunCoup" id="A0A2K2DUA7">
        <property type="interactions" value="3264"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DUA7"/>
    <dbReference type="EnsemblPlants" id="PNT77862">
        <property type="protein sequence ID" value="PNT77862"/>
        <property type="gene ID" value="BRADI_1g69780v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77863">
        <property type="protein sequence ID" value="PNT77863"/>
        <property type="gene ID" value="BRADI_1g69780v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77862">
        <property type="protein sequence ID" value="PNT77862"/>
        <property type="gene ID" value="BRADI_1g69780v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77863">
        <property type="protein sequence ID" value="PNT77863"/>
        <property type="gene ID" value="BRADI_1g69780v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3237269at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DUA7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090599">
        <property type="term" value="F:alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06603">
        <property type="entry name" value="GH31_GANC_GANAB_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000046">
        <property type="entry name" value="Glucosidase alpha, neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000039">
        <property type="entry name" value="Glucosidase, alpha neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033403">
        <property type="entry name" value="DUF5110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF54">
        <property type="entry name" value="BCDNA.GH04962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17137">
        <property type="entry name" value="DUF5110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="13">
        <location>
            <begin position="4"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="12">
        <location>
            <begin position="269"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="15">
        <location>
            <begin position="605"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5110" evidence="14">
        <location>
            <begin position="711"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="PNT77863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="834" mass="94231" checksum="5797B08FD452D6C5" modified="2018-03-28" version="1" fragment="single">PLLLRLSALPPHALRLQIDEDYSTSTPPNRRFRVPDVLLPDVDSRKLHLSKPKTVDGVSTVALSSDLDVVVKHDPFELTVRRAGSGNPVLSFNSHGLFDFEPLQESKPEGETWEEHFRSHTDSRPRGPQSITFDVSFHGADFVYGLPEHGSTSLALRPTRGPGVEESEPYRLFNLDVFEYLHESPFGLYGSIPFMIAHGAGASSGFFWLNAAEMQIDVLAPGWDGTASAENGRIDTLWMAEAGVVDAFFFVGSEPKDVIKQYISVTGAPSMPQQFAVAYHQCRWNYRDEEDVAGVDSGFDEHDIPYDVLWLDIEHTDGKRYFTWDRSTFPNPEEMQRKIADKGRKMVTIVDPHMKRDSGYYLHEEATAKGYYVKDASGKDYDGWCWPGASSYPDMLNPEIRDWWADKFSYQNYKGSTPTLYIWNDMNEPSVFNGPEVTMPRDIIHYGNVEHRELHNAYGYYFHMATSDGLLKRGEGKDRPFVLSRAFFAGSQRYGAVWTGDNTADWDHLKSSIPMVLTLGLTGMTFSGADIGGFFGNPEPDLLVRWYQVGAFYPFFRGHAHHDTKRREPWLFGERRTALMREAIHMRYSLLPYYYTLFKEASITGVPVMRPLWLEFPDDKETYNNGEAFMVGPSILAQGIYEEGQKSVSVYLPGKESWYDLRNGSPYKGSVSHKLEVSEDSIPSFLRAGTIVPRKDRFRRSSTQMVNDPYTLVIALNSSGAAEGELYVDDGKSYDYQQGAFIHRRFVFADNKLTSTNIAPNHPGKKEFSTECVIERIIVLGLSSRAKKAVIEPGNQEVEIELGPISLRSGSSSVAPTVRRPNVRVVDDWSVRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DV54</accession>
    <name>A0A2K2DV54_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g74506v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DV54"/>
    <dbReference type="FunCoup" id="A0A2K2DV54">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT78155">
        <property type="protein sequence ID" value="PNT78155"/>
        <property type="gene ID" value="BRADI_1g74506v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78155">
        <property type="protein sequence ID" value="PNT78155"/>
        <property type="gene ID" value="BRADI_1g74506v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DV54"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036043463" evidence="1">
        <location>
            <begin position="27"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT78155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="69" mass="7559" checksum="E257008C4300645F" modified="2018-03-28" version="1">MACKTTVVIASILLLALLMSCDTVQCKCKDDRTNRPCHDSATCDQDCRLEGNNRGYCDAGLCHCVDCGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I1GKM8</accession>
    <name>I1GKM8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">ATP-dependent DNA helicase 2 subunit KU80</fullName>
            <ecNumber evidence="3 15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100830366</name>
        <name evidence="17" type="ORF">BRADI_1g01007v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Single-stranded DNA-dependent ATP-dependent helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ku80 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 15"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559791.1">
        <property type="nucleotide sequence ID" value="XM_003559743.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GKM8"/>
    <dbReference type="STRING" id="15368.I1GKM8"/>
    <dbReference type="EnsemblPlants" id="KQK12012">
        <property type="protein sequence ID" value="KQK12012"/>
        <property type="gene ID" value="BRADI_1g01007v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830366"/>
    <dbReference type="Gramene" id="KQK12012">
        <property type="protein sequence ID" value="KQK12012"/>
        <property type="gene ID" value="BRADI_1g01007v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830366"/>
    <dbReference type="eggNOG" id="KOG2326">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010975_2_2_1"/>
    <dbReference type="OMA" id="WAMQYVW"/>
    <dbReference type="OrthoDB" id="30826at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GKM8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00873">
        <property type="entry name" value="KU80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000006">
        <property type="entry name" value="ATP-dependent DNA helicase 2 subunit KU80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000102">
        <property type="entry name" value="ATP-dependent DNA helicase 2 subunit KU80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1600.10:FF:000002">
        <property type="entry name" value="X-ray repair cross-complementing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.240:FF:000001">
        <property type="entry name" value="X-ray repair cross-complementing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.240">
        <property type="entry name" value="Ku, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024193">
        <property type="entry name" value="Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005160">
        <property type="entry name" value="Ku_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036494">
        <property type="entry name" value="Ku_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014893">
        <property type="entry name" value="Ku_PK_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF4">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03730">
        <property type="entry name" value="Ku_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08785">
        <property type="entry name" value="Ku_PK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016570">
        <property type="entry name" value="Ku80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101420">
        <property type="entry name" value="C-terminal domain of Ku80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ku" evidence="16">
        <location>
            <begin position="275"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR016570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK12012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="689" mass="77766" checksum="12C63FE5F239E83D" modified="2012-06-13" version="1">MARNKEGLVLLLDVGPSMHGALQEVENVCSTLLRKKLVYNRSDEVGIVLFGTKETCNDLAKEIGGYKHVVVKHDIKVVEEGTKDALQNLPRGTAPGDFLDAIVVGLDMLIKRFGNTKGKHRLCLITDSQHPLRDPPEGTKEDQVDTIAEQMKKHDIKMDCIVFRESGVQHNSVMDENDQLLYHFRDRSVAKVVQVDTPTSLLGALKTRIVLPVTIFRGDLEVSSNLKIKVWVYKKTSEEKFPTLKKYSDQAPPSDKFASHEVKVDFEYKSILEPDTVVPPDQRIKGYLYGPQVVPISSAEWEAVKFKPEKGVKLLGFADRSSIPRHYFMKDVNSFVPEPGNKKATVAVSAIARAMQEMNKVAILRCVWRQGQGNVAFGVLTPNISSVNNVPDSFFFNILPFSEDIREFQFRSFSSLPSSSQPTEEQQEAADSLVKMLDLAPPGREEILRPDFTPNPMLERFYSFLDLKSKEPDANAPPLDKCLRRITEPDSDIIDYQAPLIQNLGKAFELKDNPKRKKARTQERLNYTAADDQTKKSADPYAEKAKAIEVLYPSAEKVGKIGDLNPVQDFEAMLTERSSSIWVQKAIEEMQKYTTVLIENSPQGDYYQKALECIVALRKACIIEQEPNEFNQFLTKLYKRLKNVDDVAKFFQLLSSKNVSLISKEEAPDSDVTEEMARSFYLKPEASSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GM97</accession>
    <name>I1GM97_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">40S ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100828019</name>
        <name evidence="8" type="ORF">BRADI_1g05670v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557546.1">
        <property type="nucleotide sequence ID" value="XM_003557498.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GM97"/>
    <dbReference type="STRING" id="15368.I1GM97"/>
    <dbReference type="EnsemblPlants" id="KQK12728">
        <property type="protein sequence ID" value="KQK12728"/>
        <property type="gene ID" value="BRADI_1g05670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828019"/>
    <dbReference type="Gramene" id="KQK12728">
        <property type="protein sequence ID" value="KQK12728"/>
        <property type="gene ID" value="BRADI_1g05670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828019"/>
    <dbReference type="eggNOG" id="KOG3311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_0_1_1"/>
    <dbReference type="OMA" id="SYKGVRH"/>
    <dbReference type="OrthoDB" id="564720at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.910.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003140">
        <property type="entry name" value="PRK04053.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK12728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17713" checksum="7CB1A91703FEB267" modified="2012-06-13" version="1">MSLIAGEEFQHILRLLNTNVDGKQKIMFALTSIKGVGRRFSNIVCKKADIDMNKRAGELTPEELDRLMHVVANPRQFKVPDWFLNRKKDYKDGRFSQVVSNALDMKLRDDLERLKKIRNHRGLRHYWGVRVRGQHTKTTGRRGKTVGVSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMG5</accession>
    <name>I1GMG5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC100827610</name>
        <name evidence="21" type="ORF">BRADI_1g06270v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">Bd21</strain>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228730.1">
        <property type="nucleotide sequence ID" value="XM_010230428.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMG5"/>
    <dbReference type="STRING" id="15368.I1GMG5"/>
    <dbReference type="EnsemblPlants" id="KQK12833">
        <property type="protein sequence ID" value="KQK12833"/>
        <property type="gene ID" value="BRADI_1g06270v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827610"/>
    <dbReference type="Gramene" id="KQK12833">
        <property type="protein sequence ID" value="KQK12833"/>
        <property type="gene ID" value="BRADI_1g06270v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827610"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_37_1_1"/>
    <dbReference type="OMA" id="MGKMGQH"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009931">
        <property type="term" value="F:calcium-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000015">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000249">
        <property type="entry name" value="Calcium-dependent protein kinase SK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="127"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="428"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="464"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="500"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="536"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="28"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQK12833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="590" mass="64593" checksum="152055E2324A7BC9" modified="2012-06-13" version="1">MGNNCVGPNAPGRNGFLASVAIWRPAAARPDPAAPAPDAPPQTTFSDKAPEPVTIPASEHSSHHSSRAADPPPPKAAEPQPAQAQTQAPAPAAKKPVPKVKRVQSAGLQADSVLKRDVNTARLKDLYTIGKKLGQGQFGTTYLCVEKATGKEFACKSIAKRKLLTEEDVEDVRREIQIMHHLAGHSNVVSIVGAYEDAVAVQLVMELCAGGELFDRIIQRGHYSEKAAAQLTRVIVGVVEACHSLGVMHRDLKPENFLFVNQNEDSPLKAIDFGLSIFFKPGQMFTDVVGSPYYVAPEVLLKHYGREVDVWSAGVIIYILLSGVPPFWDESEQGIFEQVLKGELDFSTDPWPSISESAKDLVRKMLIRDPKKRLTAHEALCHPWVCVDGVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLKEMFKMLDTDNSGHITLEELKSGLQRVGATLMDSEIDALMEAADIDNSGTIDYGEFIAATMHINKVDKEDKLFTAFSYFDKDGSGYITQDELQKACEEFGIGDTHLEDIIGDVDKDNDGQIDYNEFVEMMQKGNNPLGRKGQQSNVNFGLGDALKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPV7</accession>
    <name>I1GPV7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dehydrin 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100832107</name>
        <name evidence="2" type="ORF">BRADI_1g13330v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561646.1">
        <property type="nucleotide sequence ID" value="XM_003561598.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231882.1">
        <property type="nucleotide sequence ID" value="XM_010233580.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPV7"/>
    <dbReference type="STRING" id="15368.I1GPV7"/>
    <dbReference type="EnsemblPlants" id="KQK13912">
        <property type="protein sequence ID" value="KQK13912"/>
        <property type="gene ID" value="BRADI_1g13330v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13913">
        <property type="protein sequence ID" value="KQK13913"/>
        <property type="gene ID" value="BRADI_1g13330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832107"/>
    <dbReference type="Gramene" id="KQK13912">
        <property type="protein sequence ID" value="KQK13912"/>
        <property type="gene ID" value="BRADI_1g13330v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13913">
        <property type="protein sequence ID" value="KQK13913"/>
        <property type="gene ID" value="BRADI_1g13330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832107"/>
    <dbReference type="eggNOG" id="ENOG502S99J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144452_1_0_1"/>
    <dbReference type="OMA" id="TLHMGGH"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039285">
        <property type="entry name" value="HIRD11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34941">
        <property type="entry name" value="DEHYDRIN HIRD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34941:SF1">
        <property type="entry name" value="DEHYDRIN HIRD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK13913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11313" checksum="06680502DC2957D4" modified="2012-06-13" version="1">MAGIIHKIEEKLHMGGGSDHKDEHKKEEEHKKKDGEHKKDGEHKEGMMEKIKDKITGEHGDGDKSSGDHKEKKDKKKKKEKKHGEGHKKEDGHSSSSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1GR83</accession>
    <name>I1GR83_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FKB95-like N-terminal Kelch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100833357</name>
        <name evidence="2" type="ORF">BRADI_1g18040v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024310959.1">
        <property type="nucleotide sequence ID" value="XM_024455191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GR83"/>
    <dbReference type="EnsemblPlants" id="KQK14681">
        <property type="protein sequence ID" value="KQK14681"/>
        <property type="gene ID" value="BRADI_1g18040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833357"/>
    <dbReference type="Gramene" id="KQK14681">
        <property type="protein sequence ID" value="KQK14681"/>
        <property type="gene ID" value="BRADI_1g18040v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032521_1_2_1"/>
    <dbReference type="OMA" id="WMHGNAV"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22152">
        <property type="entry name" value="F-box_AtAFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050354">
        <property type="entry name" value="F-box/kelch-repeat_ARATH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057499">
        <property type="entry name" value="Kelch_FKB95"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24414">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN SKIP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24414:SF23">
        <property type="entry name" value="F-BOX_KELCH-REPEAT PROTEIN SKIP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25210">
        <property type="entry name" value="Kelch_FKB95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FKB95-like N-terminal Kelch" evidence="1">
        <location>
            <begin position="103"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="378" mass="39632" checksum="E1F85DA42A8F395B" modified="2012-06-13" version="1">MSAAGSTSSPEPSEAVRGSLIPVLPDDLAVHCIARLPRAAHPALALVSRALHALLCRHPEPLLAARKRLGLSDPHVLVSLRPPSSASPLFFLLLPQPGWPPLPLPTPPVPVSSSSAAAAAGSRLFLVGGSVDGVPAPSVQVLDARTRSWSVGPRLSSPREFAAAVALPGALFVAGGCVPSSPFWAESLDLASPRAKWAPVPSPDHLREKWIHGCVSLAGKVLAVADRGGLAYDPAAPPAEAWAPVSPVLDMGWKGRAAVVGGILYSYDYMGQVKGYDPDTDAWSMVQGLDKELPRFLCGATLANVGGLLYLIWEVKWKGKASASDGEVRGMVAVEWATIEVTSAKEGRLMGKVMCRDTVVFADMPRGSAITHCVALDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSD6</accession>
    <name>I1GSD6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein TIFY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Jasmonate ZIM domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100828668</name>
        <name evidence="9" type="ORF">BRADI_1g21490v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Repressor of jasmonate responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The jas domain is required for interaction with COI1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TIFY/JAZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562699.1">
        <property type="nucleotide sequence ID" value="XM_003562651.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSD6"/>
    <dbReference type="FunCoup" id="I1GSD6">
        <property type="interactions" value="1074"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GSD6"/>
    <dbReference type="EnsemblPlants" id="KQK15259">
        <property type="protein sequence ID" value="KQK15259"/>
        <property type="gene ID" value="BRADI_1g21490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828668"/>
    <dbReference type="Gramene" id="KQK15259">
        <property type="protein sequence ID" value="KQK15259"/>
        <property type="gene ID" value="BRADI_1g21490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828668"/>
    <dbReference type="eggNOG" id="ENOG502RIU4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051749_1_1_1"/>
    <dbReference type="OMA" id="FPRDHVS"/>
    <dbReference type="OrthoDB" id="1937734at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GSD6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018467">
        <property type="entry name" value="CCT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040390">
        <property type="entry name" value="TIFY/JAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010399">
        <property type="entry name" value="Tify_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077:SF140">
        <property type="entry name" value="PROTEIN TIFY 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09425">
        <property type="entry name" value="Jas_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06200">
        <property type="entry name" value="tify"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00979">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51320">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Tify" evidence="8">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK15259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="237" mass="25028" checksum="028795119385B644" modified="2012-06-13" version="1">MAASTRPGEKASSFAVACSLLSRYVRQNGAAAAELSLGFNKVSGEAETQKEAATKSSLPVVDGEESGRKKETMELFPQSAGLGGVQDAATPDAIAREQEKRQLTIFYGGKVLVFNDFPADMAKNLIQLASKGNPVVQNTTVPTPVIDSNKAETVVQAPASSLPGAQNDAPKPARPNAADLPIARKASLHRFLEKRKDRLHASAPYQASPSDATPVKKEPENQTWLGLGPNAAAKPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1GUH1</accession>
    <name>I1GUH1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="6">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100826830</name>
        <name evidence="8" type="ORF">BRADI_1g27867v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Extracellular space</location>
            <location evidence="6">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="6"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563122.1">
        <property type="nucleotide sequence ID" value="XM_003563074.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GUH1"/>
    <dbReference type="FunCoup" id="I1GUH1">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GUH1"/>
    <dbReference type="EnsemblPlants" id="KQK16264">
        <property type="protein sequence ID" value="KQK16264"/>
        <property type="gene ID" value="BRADI_1g27867v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826830"/>
    <dbReference type="Gramene" id="KQK16264">
        <property type="protein sequence ID" value="KQK16264"/>
        <property type="gene ID" value="BRADI_1g27867v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826830"/>
    <dbReference type="eggNOG" id="ENOG502QQC7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048041_1_1_1"/>
    <dbReference type="OMA" id="VHRAMAF"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982659" description="Xyloglucan endotransglucosylase/hydrolase" evidence="6">
        <location>
            <begin position="29"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="7">
        <location>
            <begin position="24"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK16264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="302" mass="34108" checksum="F4428537990D43E9" modified="2012-06-13" version="1">MASISLPLLPAMAAAVLLLAMAVGSSDAQPSPGYYPSSRFRPVAFNRGYKNKWGPQHQTLSGDQSALTIWLDKTCGSGFKSKHSYRNGYFATRIKLPSGYTAGTNTAFYLSNNEAHPGFHDEIDMEFLGTIPGEPYTLQTNVYVRGSGDGRIIGREMRFHLWFDPTAGFHHYAILWNPDAITFFVDDVPIRRYERKTELTFPDREMWAYGSIWDASDWATDDGRHRADYRYQPFVARFDRFVVAGCATTAPASCRPVPASGPRGSTGLTPRQYAAMRWAQQGHMVYYYCQDFRRDRSLTPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1GX00</accession>
    <name>I1GX00_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100835700</name>
        <name evidence="3" type="ORF">BRADI_1g35070v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563561.1">
        <property type="nucleotide sequence ID" value="XM_003563513.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GX00"/>
    <dbReference type="EnsemblPlants" id="KQK17527">
        <property type="protein sequence ID" value="KQK17527"/>
        <property type="gene ID" value="BRADI_1g35070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835700"/>
    <dbReference type="Gramene" id="KQK17527">
        <property type="protein sequence ID" value="KQK17527"/>
        <property type="gene ID" value="BRADI_1g35070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835700"/>
    <dbReference type="eggNOG" id="KOG2819">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032056_0_0_1"/>
    <dbReference type="OMA" id="YRKNDQK"/>
    <dbReference type="OrthoDB" id="411211at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039156">
        <property type="entry name" value="PHAF1/BROMI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465:SF2">
        <property type="entry name" value="PHAGOSOME ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465">
        <property type="entry name" value="UPF0183 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK17527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="426" mass="47134" checksum="B15B75D06726E3A3" modified="2012-06-13" version="1">MQAQRSPAMVGGGAGAAVGSPSPATVPVRRRCEGTAMGAITLDLRPGLGVGPFTLGMPISDAFAQIEHQPNIYDVVHVKYFDEEPLELDLVISFPDHGFHLRFDTWSQRLRLIEIFDVKRLQLRYATSLIGGPSTLATFVSVYALFGPTFPGIYDKERGIYTLFYPGLSFAFPIPSQYTNFFTNGEVADLPLEFPDGTTPVTCRVSIYDSSTDSKVGVGSLMDKAVVPALPVGSLYMEEVHAKLGEELWFTTGGQHIPFGASPQDIWTELGRPCGIHQKQVDQMVIHSASDLRPRTTLCGDYFYNYFSRGIDILFDGQTHRIKKFVLHTNFPGHSDFNSYMKCNFVIYDAEAEGTQQVGNISKNCITPRTKWEQVKEILGDCGRAAIQTQGSMNNPFGSTFVYGYQNIAFEVMKNGYIATVTLFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1GY54</accession>
    <name>I1GY54_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 20">Peroxidase</fullName>
            <ecNumber evidence="4 20">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC100828172</name>
        <name evidence="23" type="ORF">BRADI_1g38350v3</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">Bd21</strain>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17 20">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="17 20">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17 20">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="17 20">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 20"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563785.1">
        <property type="nucleotide sequence ID" value="XM_003563737.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GY54"/>
    <dbReference type="STRING" id="15368.I1GY54"/>
    <dbReference type="EnsemblPlants" id="KQK18061">
        <property type="protein sequence ID" value="KQK18061"/>
        <property type="gene ID" value="BRADI_1g38350v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828172"/>
    <dbReference type="Gramene" id="KQK18061">
        <property type="protein sequence ID" value="KQK18061"/>
        <property type="gene ID" value="BRADI_1g38350v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828172"/>
    <dbReference type="eggNOG" id="ENOG502QQ2M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010543_0_0_1"/>
    <dbReference type="OMA" id="ARCHHYR"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS30 family.</text>
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    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="62" mass="6913" checksum="D19C19A092F5235F" modified="2012-06-13" version="1">MGKVHGSLARAGKVRGQTPKVAKQDKKKQPRGRAHKRIQYNRRFVTAVVGFGKKRGPNSSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4H3</accession>
    <name>I1H4H3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100826582</name>
        <name evidence="4" type="ORF">BRADI_1g59620v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557707.1">
        <property type="nucleotide sequence ID" value="XM_003557659.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H4H3"/>
    <dbReference type="STRING" id="15368.I1H4H3"/>
    <dbReference type="EnsemblPlants" id="KQK21241">
        <property type="protein sequence ID" value="KQK21241"/>
        <property type="gene ID" value="BRADI_1g59620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826582"/>
    <dbReference type="Gramene" id="KQK21241">
        <property type="protein sequence ID" value="KQK21241"/>
        <property type="gene ID" value="BRADI_1g59620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826582"/>
    <dbReference type="eggNOG" id="KOG1463">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029573_2_1_1"/>
    <dbReference type="OMA" id="MCLECIE"/>
    <dbReference type="OrthoDB" id="1418352at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000007">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040780">
        <property type="entry name" value="Rpn6_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040773">
        <property type="entry name" value="Rpn6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18503">
        <property type="entry name" value="RPN6_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18055">
        <property type="entry name" value="RPN6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="229"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK21241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="428" mass="47776" checksum="E9EFCB06410B7DEC" modified="2012-06-13" version="1">MTMSSPMQSSFLPATTESIAMAQEAKDASESISILYRVLEDPSSSSDAVRTKELAITNLTTYLTKENRAEELRNLLTQLRPFFALIPKAKTAKIVRGIIDAVAKIPGTSDLQISLCKEMVEWTRAEKRTFLRQRVEARLASLLLESQEYTEALALLSGLIKEVRRLDDKLLLVDIDLLESKLHFSLRNLPKAKASLTAARTAANAIYVPPAQQGTIDLQSGILHAEEKDYKTAYSYFFEAFEGFNALEDAKAIFCLKYMLLCKIMVNQADDVAGIISSKAGLKYVGPDVDAMKAVADAYSKRSLKYFETALRDYKAQLEEDPIVHRHLSSLYDTLLEQNLCRLIEPYSRVEIAHVAEMIELPIDHVEKKLSQMILDKKFAGTLDQGAGCLVIFEDPKTEAIFPATLETISNVGKVVESLYMRSAKIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1H8W2</accession>
    <name>I1H8W2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100827926</name>
        <name evidence="4" type="ORF">BRADI_1g72250v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558644.1">
        <property type="nucleotide sequence ID" value="XM_003558596.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H8W2"/>
    <dbReference type="STRING" id="15368.I1H8W2"/>
    <dbReference type="EnsemblPlants" id="KQK23252">
        <property type="protein sequence ID" value="KQK23252"/>
        <property type="gene ID" value="BRADI_1g72250v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK23253">
        <property type="protein sequence ID" value="KQK23253"/>
        <property type="gene ID" value="BRADI_1g72250v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT78023">
        <property type="protein sequence ID" value="PNT78023"/>
        <property type="gene ID" value="BRADI_1g72250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827926"/>
    <dbReference type="Gramene" id="KQK23252">
        <property type="protein sequence ID" value="KQK23252"/>
        <property type="gene ID" value="BRADI_1g72250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK23253">
        <property type="protein sequence ID" value="KQK23253"/>
        <property type="gene ID" value="BRADI_1g72250v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78023">
        <property type="protein sequence ID" value="PNT78023"/>
        <property type="gene ID" value="BRADI_1g72250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827926"/>
    <dbReference type="eggNOG" id="KOG3381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075876_3_0_1"/>
    <dbReference type="OMA" id="NQCISAR"/>
    <dbReference type="OrthoDB" id="2746at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H8W2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000010">
        <property type="entry name" value="Protein AE7-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="3">
        <location>
            <begin position="39"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK23252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="158" mass="17789" checksum="FDE692F7D0D0C739" modified="2012-06-13" version="1">MVMELSNANPVVYEKKERRIRQAPENTDENAAEPIDQLEIFDHIRDIKDPEHPYSLEDLNVVTEESVEINDELSHVKVTFTPTVEHCSMATVIGLCLRVKLMRSLPPRYKVDIRVAPGSHATEAAVNKQLSDKERVAAALENPNLLDIVEECLSPTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9Q8</accession>
    <name>I1H9Q8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100827010</name>
        <name evidence="10" type="ORF">BRADI_1g75170v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558810.1">
        <property type="nucleotide sequence ID" value="XM_003558762.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H9Q8"/>
    <dbReference type="STRING" id="15368.I1H9Q8"/>
    <dbReference type="EnsemblPlants" id="KQK23650">
        <property type="protein sequence ID" value="KQK23650"/>
        <property type="gene ID" value="BRADI_1g75170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827010"/>
    <dbReference type="Gramene" id="KQK23650">
        <property type="protein sequence ID" value="KQK23650"/>
        <property type="gene ID" value="BRADI_1g75170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827010"/>
    <dbReference type="eggNOG" id="KOG0416">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_7_1_1"/>
    <dbReference type="OMA" id="LMKLMMR"/>
    <dbReference type="OrthoDB" id="269518at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H9Q8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000024">
        <property type="entry name" value="Ubiquitin-conjugating enzyme 5, E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK23650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="185" mass="21072" checksum="3CFE705FF8BCF266" modified="2012-06-13" version="1">MSSPSKRREMDLMKLMMRDYKVEMVNDGMQEFFIEFRGPNESLYQGGVWKVRVEFPDAYPYRSPSVGFCNKIYHPNVDELSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAALLMRDRAAYEQKVKECCEKYAKPEDAGITPEDNSSEEEPSEEADDDSGDDEPILGHPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9Y7</accession>
    <name>I1H9Y7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100840053</name>
        <name evidence="2" type="ORF">BRADI_1g75850v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558853.1">
        <property type="nucleotide sequence ID" value="XM_003558805.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H9Y7"/>
    <dbReference type="STRING" id="15368.I1H9Y7"/>
    <dbReference type="EnsemblPlants" id="KQK23750">
        <property type="protein sequence ID" value="KQK23750"/>
        <property type="gene ID" value="BRADI_1g75850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840053"/>
    <dbReference type="Gramene" id="KQK23750">
        <property type="protein sequence ID" value="KQK23750"/>
        <property type="gene ID" value="BRADI_1g75850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840053"/>
    <dbReference type="eggNOG" id="ENOG502SFAF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2350071_0_0_1"/>
    <dbReference type="OMA" id="REFQPRS"/>
    <dbReference type="OrthoDB" id="773814at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33401">
        <property type="entry name" value="LIGHT-HARVESTING COMPLEX-LIKE PROTEIN OHP2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33401:SF2">
        <property type="entry name" value="OS03G0138400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK23750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="120" mass="12439" checksum="46FD9B0285AEC8C6" modified="2012-06-13" version="1">MDRRSFRSSSCNGGGKNPVPAPSPAAAGSRTSAVPAAAGSRTSASPAAGSKEDAGAGERKALLPRHLPGGTARKGQKGPKRRVQWKDSHGKKLAEVLEFQPSDSSDSDDEYLDTCICTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAS0</accession>
    <name>I1HAS0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100837925</name>
        <name evidence="14" type="ORF">BRADI_1g78140v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559018.1">
        <property type="nucleotide sequence ID" value="XM_003558970.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAS0"/>
    <dbReference type="STRING" id="15368.I1HAS0"/>
    <dbReference type="EnsemblPlants" id="KQK24097">
        <property type="protein sequence ID" value="KQK24097"/>
        <property type="gene ID" value="BRADI_1g78140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837925"/>
    <dbReference type="Gramene" id="KQK24097">
        <property type="protein sequence ID" value="KQK24097"/>
        <property type="gene ID" value="BRADI_1g78140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837925"/>
    <dbReference type="eggNOG" id="KOG3002">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040603_1_0_1"/>
    <dbReference type="OMA" id="SCSYAKW"/>
    <dbReference type="OrthoDB" id="4788989at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HAS0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16571">
        <property type="entry name" value="RING-HC_SIAHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044286">
        <property type="entry name" value="SINL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46632:SF16">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46632">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SIAH-type" evidence="13">
        <location>
            <begin position="148"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK24097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="329" mass="35036" checksum="98E0D96C25515A8C" modified="2012-06-13" version="1">MAKFSFDDADEDPPAAAAAGAGGDKRKREGSAAADGADGGGPPPTSKARNSAVVGGERDGRAVVGASCCERRVDGDGEPGTGGISMRIDTDLLDCSICFEPLSPPLYQCQNGHVACFSCWSRLSNKCHVCSHDAIFARNIALEKIVESIKSSCAYAKWGCSKLVSYAQRSVHEEACLFAPSTCPIPGCGYRGFTGCWSGHFLVDHSADCLHFTYGQSFEVNLAVSLPFLVLLGEDDHLFLLLNKNMMPFGHAFTVVCLRNGNLNWNFSYEIEAASRGNPGNCLRLKASVTNTKEWGGLHPAEAFLLVPYAFCSSANQTLSVSVARSASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1HKS1</accession>
    <name>I1HKS1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100824588</name>
        <name evidence="8" type="ORF">BRADI_2g31720v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566411.1">
        <property type="nucleotide sequence ID" value="XM_003566363.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HKS1"/>
    <dbReference type="STRING" id="15368.I1HKS1"/>
    <dbReference type="EnsemblPlants" id="KQK06957">
        <property type="protein sequence ID" value="KQK06957"/>
        <property type="gene ID" value="BRADI_2g31720v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824588"/>
    <dbReference type="Gramene" id="KQK06957">
        <property type="protein sequence ID" value="KQK06957"/>
        <property type="gene ID" value="BRADI_2g31720v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824588"/>
    <dbReference type="eggNOG" id="ENOG502QQ2H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015477_2_0_1"/>
    <dbReference type="OMA" id="IAMNAQV"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF9">
        <property type="entry name" value="METAL-NICOTIANAMINE TRANSPORTER YSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="436"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="553"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="593"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="623"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK06957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="684" mass="74582" checksum="1BCC9FF23E86A55B" modified="2012-06-13" version="1">MDAMISDPSLAMSVERAFEKQPLAGTLDQVTPRSVVIAVVLSIVFGFVSLKLQMTAGVLPSLNMPITVLSYIFLKWFVGLLRTCGLPAPPCTRQETVLIVTTVITVGNLAITGGFALYITSMTSVVATSLGEEHPDPKNIVDNFPTGQWMLFLFLVGLMGIFTSVPLNQVMILDYRLLFPTGTAQAHLINSFHTPQGAYLAKMQVASIFKFFIGSFSWAAFSWLYTAGKDCGFSSFPLFGLELYKHRFFFDFSATYIGVGMMVPPMVNISFLSGGMVSWGILFPFLESKNGIWYNASPTSLSGLNGYKIFITVALIVTDGLYTLIMLLITAYNDYRLKRQETDSVVANYLKRHPSLNYDDRKRIEVFLENRIPVSIPVVGYIICATICAVIIPNLFSQISFYHVITIYTIIPMAAVTNTYVAGLTDWNVAYTYAKFTIFIVAAWVAKPGAIVAGLVACGIVVTSLYISSLTTMDIKTGYMTLTSQRAMVFMQIVGITIGSVITPCIFRAFQRSGKAHVPIGSADSAYPCPYAGLYRAIGMIGSAGVDALPKHCLKFCFAAACFAIAINTISLLSQRKGWAIQAYIPSVTVFALPFTIGSAVGIDLCIGSVVMFIWTKMNVQSAVLLSAAVASGLMCGEGLFTFPSALLSLYSVEPPMCMKFIHSGKQVDLTESFFRNMGTPTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HL99</accession>
    <name>I1HL99_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100842133</name>
        <name evidence="3" type="ORF">BRADI_2g34040v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatase 2A regulatory subunit.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315059.1">
        <property type="nucleotide sequence ID" value="XM_024459291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HL99"/>
    <dbReference type="STRING" id="15368.I1HL99"/>
    <dbReference type="EnsemblPlants" id="KQK07230">
        <property type="protein sequence ID" value="KQK07230"/>
        <property type="gene ID" value="BRADI_2g34040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842133"/>
    <dbReference type="Gramene" id="KQK07230">
        <property type="protein sequence ID" value="KQK07230"/>
        <property type="gene ID" value="BRADI_2g34040v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2085">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012437_4_1_1"/>
    <dbReference type="OMA" id="FRRIAYC"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051177">
        <property type="term" value="P:meiotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000306">
        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF64">
        <property type="entry name" value="SERINE_THREONINE PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK07230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="487" mass="55564" checksum="C464D3898841245C" modified="2012-06-13" version="1">MWKQFLGKLSGKSPKSGGVGAGGWGSPPAKPPTSYGANGVQWDSMRASPPLAGVAGAEGETREDVFLQKLNVCCVLFDFSTDRGRDSPERERKRHVLLSLVDCLGAAEEPLTEAMVSACVRMFAINLFRVFPPKVRPGGVGTTEADEDDPFFDPSWYHLQVVYELLLRFVTSPVVDVKVARKYMDNSFISRLLDLLDSDDPRERDCLKTVLHRIYGKFMGNRPFIRKAVSNIFYRFVSETDRHNGIAELLEVFGSVISGFAKPLKEEHKLFLWKALIPLHKPKTVGMYLPQLTYCITQFIDKEPKLSGTVIRGLLKYWPVTNSQKEMMFLGELEEVLELTEMPEFQKCMVPLFRRVAHCLNSSHFQVAERALYLWNNEHLFDLISQNHQVILPIIYPALERNARLHWNQSVLNVTMNVRKMFFDMDQKLLLACEINLQEEEEKQAASEERRRLIWEHLERNAVYHPVTGDINFAVLPTSALVAPTMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQR2</accession>
    <name>I1HQR2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100834497</name>
        <name evidence="10" type="ORF">BRADI_2g47590v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569634.1">
        <property type="nucleotide sequence ID" value="XM_003569586.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQR2"/>
    <dbReference type="EnsemblPlants" id="KQK09372">
        <property type="protein sequence ID" value="KQK09372"/>
        <property type="gene ID" value="BRADI_2g47590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834497"/>
    <dbReference type="Gramene" id="KQK09372">
        <property type="protein sequence ID" value="KQK09372"/>
        <property type="gene ID" value="BRADI_2g47590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834497"/>
    <dbReference type="eggNOG" id="KOG0048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028567_16_4_1"/>
    <dbReference type="OMA" id="GRHACSA"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000158">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000268">
        <property type="entry name" value="Transcription factor MYB86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051953">
        <property type="entry name" value="Plant_SW-associated_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997:SF1">
        <property type="entry name" value="OS01G0702700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="64"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="64"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK09372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="283" mass="31347" checksum="5C77E2CCB13D9893" modified="2012-06-13" version="1">MGRHAGTGGVQQKLRKGLWSPEEDEKLYNHIIRYGVGCWSSVPKLAGLQRCGKSCRLRWINYLRPDLKRGSFSQQEEDAIVGLHEILGNRWSQIASHLPGRTDNEIKNFWNSCLKKKLRQRGIDPSTHKPISSAAAAVETDQAPKDQKPQTAVEGFSTLKQQQVFDPFPVTDTFNGGFDGVGMTLYDNLGGKDASGFVDYSSVLDVSENLGYGESSSNSSNWNCAPEVNNVLEGHWASESKAEPFAGYGGGEQDEALEHKFVLPCQGGQEHSMAHFDFNLEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSK9</accession>
    <name>I1HSK9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100830929</name>
        <name evidence="10" type="ORF">BRADI_2g52700v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315574.1">
        <property type="nucleotide sequence ID" value="XM_024459806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSK9"/>
    <dbReference type="STRING" id="15368.I1HSK9"/>
    <dbReference type="EnsemblPlants" id="KQK10209">
        <property type="protein sequence ID" value="KQK10209"/>
        <property type="gene ID" value="BRADI_2g52700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830929"/>
    <dbReference type="Gramene" id="KQK10209">
        <property type="protein sequence ID" value="KQK10209"/>
        <property type="gene ID" value="BRADI_2g52700v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_27_2_1"/>
    <dbReference type="OMA" id="EEGMGTN"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="6 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094777" description="Cytochrome P450" evidence="9">
        <location>
            <begin position="32"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK10209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="57899" checksum="84DFF6846E839991" modified="2012-06-13" version="1">MELSLTSSLAILPLLLLPLLCFLCLRWQTRKQPRADGLKAYPVVGTLPHFVKNQHRLVEWSAGIIARCPTHTMSFNFKGLGLMAGAITANPANVEYVMKTNFQNYPKGDFVVSVIEDFLGHGIFNSDGDQWLWQRKASSYEFSKRSLRNFVVSTVRFEVVERLLPLLSQAEQDGRTLDVQDVLERFAFDNICCVAFDEDPACLTEEGLGVNGRAEFMHALTDAQNIVMARFMSPIKSAWRIKKLLNIEPERRMHEALATIHGYVDRIVRERSERGAAGLARKDDFLSRFASSGEHSNESLRDVVTNFILAGRDTTSSALTWFFWLLSSRPDVEEKIVREICTVRASSRSTDAAFSFSELRKMNYLQAAITESMRLYPPVAMDSRCCQHDDVLPDGTFVGKGWQVSYSAYAMARLEEIWGNDCQEYRPERWLDAEGMFQPESTSKYPIFHGGPRMCLGKEMAYIQMKSIVACVFERFSFQFVGGESRPGLVFSVTLRMEGGLPMQVKKREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1HW10</accession>
    <name>I1HW10_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RuBisCO large subunit-binding protein subunit beta, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100842360</name>
        <name evidence="7" type="ORF">BRADI_3g00480v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574065.1">
        <property type="nucleotide sequence ID" value="XM_003574017.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233648.1">
        <property type="nucleotide sequence ID" value="XM_010235346.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317350.1">
        <property type="nucleotide sequence ID" value="XM_024461582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HW10"/>
    <dbReference type="STRING" id="15368.I1HW10"/>
    <dbReference type="EnsemblPlants" id="KQJ92756">
        <property type="protein sequence ID" value="KQJ92756"/>
        <property type="gene ID" value="BRADI_3g00480v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92757">
        <property type="protein sequence ID" value="KQJ92757"/>
        <property type="gene ID" value="BRADI_3g00480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842360"/>
    <dbReference type="Gramene" id="KQJ92756">
        <property type="protein sequence ID" value="KQJ92756"/>
        <property type="gene ID" value="BRADI_3g00480v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92757">
        <property type="protein sequence ID" value="KQJ92757"/>
        <property type="gene ID" value="BRADI_3g00480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842360"/>
    <dbReference type="eggNOG" id="KOG0356">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016503_4_1_1"/>
    <dbReference type="OMA" id="MRPQRKC"/>
    <dbReference type="OrthoDB" id="1733909at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ92757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="63657" checksum="4000FAA9E772D83B" modified="2012-06-13" version="1">MASPFGGTSTCGLKAAAPAGFATKKQLSLVLPPCVSLPQKFRPRRKCSFRVNAAKELYFNKDGSAIKKLQNGVNKLADLVGVTLGPKGRNVVLESKYGSPKIVNDGVTVAKEVELEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVILAQGMITEGVKIVAAGANPVQIARGIEKTAKALVSELRKMSKEVEDSELADVAAVSAGNNYEVGNMIAEAMSKVGRQGVVTLEEGKSAENNLYVVEGMQFDRGYISPYFVTDSEKMTVEYENCKLLLVDKKINNARDLITILEDAIKSGYPILIIAEDIEQEALATLVVNRLRGALKIAAIKAPGFGERKSQYLDDIATLTGGTVIREEIGLSLDKADSEVLGTAAKVLVTKDTTTIVGDGSTQEEVSKRVTQIKNQIEAAEQEYEKEKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLAAKVDAIKETLENDEQKIGAEIVRKSLSYPLKLIAKNAGVNGSVVTEKVLANDNFRYGYNAATGKYEDLMAAGIIDPTKVVRCCLEHAASVAKTFITSDAVVVDIKEAEQAPAANPMAGSGYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HX48</accession>
    <name>I1HX48_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100830650</name>
        <name evidence="8" type="ORF">BRADI_3g03637v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233798.1">
        <property type="nucleotide sequence ID" value="XM_010235496.3"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HX48"/>
    <dbReference type="EnsemblPlants" id="KQJ93285">
        <property type="protein sequence ID" value="KQJ93285"/>
        <property type="gene ID" value="BRADI_3g03637v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830650"/>
    <dbReference type="Gramene" id="KQJ93285">
        <property type="protein sequence ID" value="KQJ93285"/>
        <property type="gene ID" value="BRADI_3g03637v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830650"/>
    <dbReference type="eggNOG" id="KOG4521">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004612_0_1_1"/>
    <dbReference type="OMA" id="TIERLWC"/>
    <dbReference type="OrthoDB" id="67716at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059141">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021717">
        <property type="entry name" value="Nucleoporin_Nup160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035192">
        <property type="entry name" value="NUP160_hel_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056536">
        <property type="entry name" value="TPR_NUP160_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056535">
        <property type="entry name" value="TPR_NUP160_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11715">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17238">
        <property type="entry name" value="NUP160_helical_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23354">
        <property type="entry name" value="TPR_NUP160_120_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23347">
        <property type="entry name" value="TPR_Nup160_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup120/160 beta-propeller" evidence="4">
        <location>
            <begin position="69"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 helical" evidence="5">
        <location>
            <begin position="553"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 middle TPR" evidence="7">
        <location>
            <begin position="925"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 C-terminal TPR" evidence="6">
        <location>
            <begin position="1226"/>
            <end position="1507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ93285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1522" mass="170620" checksum="24899601BEAAB9BC" modified="2012-06-13" version="1">MATPAPAPASRRIAGTEVPITGSDKIRWIDLTVPSSSSAPESATDPFVSVPPRAASGCHIVYPGSDSQRYLAWRIHEEHQNVLEVIELCASKEFPSSGLRLIFEEELCPFAFICESEGARRDKSVYLLYVVTVSGVAFLCNLHSPFSYVSGSILAQNDIVEFSLQTLTQSAKVTAVKAKPGCLVIGRQDGSICCYSLGKVAPCSPGFSNELRDDAGIGRLWTLMSRTKAVGPVQDIETAIVNERELLFVLHLDGSLRVWDIFSHTKVLNYNVQSNGFEGQPSRLWVGEADDDQELITLAVLHQGTVVPACDRVAVYGFSFGAGEKFLLSPEPSVFDVPLLEGKLVDLKISTEKLWILKEVGPMLYEIVQYDTDTGEMCSYVLQEDAISEQMFQSSESALDDLVWTADSIFSSTKEHSFSFISSMFLRRLLQPGVNHWCALRETLLEYKRFLSDSEFQSLTTSGLGKEILSIIEQEESSQTASSTAYHWKKFSARYLHNWCRNNRPYGLLLDTNGEVFGLIRKGSFSLFRCLERVEQLIYGFSDELGNLNGLELTILSEKSETEILIEVLTCMGHIHHLLGRSSAAIYHESLISSVISLDEIASQILKILETGFSPQSSSALIALLGTDAYVERRQIAHKSQRKFSVEMLLSFQKLQSRSISWPAVFDVIERFMKYLNTELTRQECESKRVCNINSMLLVQATSQVARTMFESAFDLFLFLRYLVGVGGQVSLLQSDAARIKLKLLPMIQDILGQWIVLHFIGISPTMPPTIEDFSYQLSSLQLGIADELSLHRKLGSSDFTLACLLDFPKSAEGDALSPCFPTPAEVINLVRRFSSSIMCRSNVEHVDGFLDTSWVHSFLGSTINLAALFVRHGQYGAAENLLGILETYWNNGRASQTDKDTDIASLARLHLNGFCLLMLAHDEANIVLKESKVHEAIRCFFRAASGQEAPKALKKFSSETGFQFSGECRSISLWRLHYYEWAMQIFEQHSMSEGACQFALAALEQVDSIFYLNNGSDAEDLPETAAMIKGRLWANVFKYSLDLKKFHDAYCAIISNTDEDSKYVCLRRFIIVLCELGETKVICSGEIPFTGLVEKVEQELFWKAERSDLSSKPNLYKVLYSFEAYRNNWRKAAAYMYRYFVRLSREGNAGGTLQLSHVLQERLHALAAAINSLQLVDPSSAWLDSVCEADDQISPSKKPRNLLMENSAFGTDSERSRLQFCVDIEILEKEYTLTEALYMLSTVNSRSNFSESYSIETLTDILINENLYDLAFTIVLKFWKDSGMKRELERVFAAIAQQCCPNKVGNSGRDLTGSQQLLLLPSSQDDAWDDNNKSIAVAHQVRGSCQWETLEIYLDKYTDLHPRLPVIVAETLLYTDPEIELPLWLVQMFKTSKGGNRMISWGMSGKEADPAALFRLYINYGRHTEATNLLVQYLDSFASSRPVDVLHRKKMSAAWFPYTTIERFWSQLEEMQSAGHSVDQCDKLKKLLHGALMNHLQQVVVDSEDVLSSVGGGQGTESQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXW0</accession>
    <name>I1HXW0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FGGY carbohydrate kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100830328</name>
        <name evidence="7" type="ORF">BRADI_3g05780v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571008.1">
        <property type="nucleotide sequence ID" value="XM_003570960.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXW0"/>
    <dbReference type="STRING" id="15368.I1HXW0"/>
    <dbReference type="EnsemblPlants" id="KQJ93624">
        <property type="protein sequence ID" value="KQJ93624"/>
        <property type="gene ID" value="BRADI_3g05780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830328"/>
    <dbReference type="Gramene" id="KQJ93624">
        <property type="protein sequence ID" value="KQJ93624"/>
        <property type="gene ID" value="BRADI_3g05780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830328"/>
    <dbReference type="eggNOG" id="KOG2517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_10_2_1"/>
    <dbReference type="OMA" id="HKAMWHE"/>
    <dbReference type="OrthoDB" id="203824at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019150">
        <property type="term" value="F:D-ribulokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019321">
        <property type="term" value="P:pentose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07782">
        <property type="entry name" value="ASKHA_NBD_FGGY_D-RBK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000101">
        <property type="entry name" value="FGGY carbohydrate kinase domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006003">
        <property type="entry name" value="FGGY_RbtK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01315">
        <property type="entry name" value="5C_CHO_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435:SF4">
        <property type="entry name" value="FGGY CARBOHYDRATE KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435">
        <property type="entry name" value="RIBULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="5">
        <location>
            <begin position="7"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="6">
        <location>
            <begin position="303"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ93624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="571" mass="61267" checksum="B243B38FE8085E87" modified="2012-06-13" version="1">MSPGGVFLGVDVGTGSARAGLFDEKGKLLGSASSPIQIWKEKDCIEQSSTDIWHAVCAAVKSACSLANVAPDGVVGLGFAATCSLVAVDADGSPVSVSWSGDARRNIIVWMDHRAVDQADRINARNSPVLQYCGGGVSPEMQAPKLLWVKENLQESWPMVCRWMDLSDWLAYRATGDDTRSLCTTVCKWTYLGHAHMEQWRESDSRDMEACGWDEVFWEEIGLGDLVEGNRAKIGRSVAFPGHPLGSGLTPTSAKELGLLPGTPVGTSLIDAHAGGVGVMESVPDAGSEAGSSDEEAICRRMVLVCGTSTCHMAVSKNKLFIPGVWGPFWSAMVPEYWLTEGGQSATGALIDYIVQNHAAAPLLAKNAASQSVSIFELMNKMLISMSHEQNLPFLSALSQDTHVLPDFHGNRSPVADPKSKGVICGLTLDTSEMHLALLYLATIQAIAYGTRHIVEHCNTNGHKIDTLLACGGLAKNSLYIQEHADIIGCPIILPRESESVLLGAAVLGAVAAKKFPGVRDAMKSLNAAGEVVHPSSDPRVKKYHDAKYQIFRSLYEQQLSHRSAMSQALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1I108</accession>
    <name>I1I108_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100842357</name>
        <name evidence="17" type="ORF">BRADI_3g15250v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>oxalate + O2 + 2 H(+) = H2O2 + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:21880"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
            <dbReference type="EC" id="1.2.3.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 15">Secreted</location>
            <location evidence="2 15">Extracellular space</location>
            <location evidence="2 15">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the germin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573398.1">
        <property type="nucleotide sequence ID" value="XM_003573350.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I108"/>
    <dbReference type="EnsemblPlants" id="KQJ95107">
        <property type="protein sequence ID" value="KQJ95107"/>
        <property type="gene ID" value="BRADI_3g15250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842357"/>
    <dbReference type="Gramene" id="KQJ95107">
        <property type="protein sequence ID" value="KQJ95107"/>
        <property type="gene ID" value="BRADI_3g15250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842357"/>
    <dbReference type="eggNOG" id="ENOG502QQ4A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015790_0_0_1"/>
    <dbReference type="OMA" id="VSWHALA"/>
    <dbReference type="OrthoDB" id="1850619at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050162">
        <property type="term" value="F:oxalate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0052">Apoplast</keyword>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 12 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094981" description="Germin-like protein" evidence="15">
        <location>
            <begin position="25"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="16">
        <location>
            <begin position="72"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ95107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="228" mass="24799" checksum="3DB8CACF9442006B" modified="2012-06-13" version="1">MASTSSYFLLLAAVLALVSWQAVASDPSPLQDFCVADNSSQVLVNGFVCKDPKDVTAEDFFLSAKLDMPRDTKMSKVGSNVTLINVMKIPGLNTLGISLARIDYAPLGENPPHTHPRATEILTVLEGTLYVGFVTSNPENKFLSKELKKGDVFVFPQGLIHFQFNPNPYKPAVAIAALSSQNPGAITIANVVFGSKPMISDDVLAKAFQVEKKTVDWLQAQFWADNHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1H2</accession>
    <name>I1I1H2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100842157</name>
        <name evidence="6" type="ORF">BRADI_3g16680v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573482.1">
        <property type="nucleotide sequence ID" value="XM_003573434.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1H2"/>
    <dbReference type="STRING" id="15368.I1I1H2"/>
    <dbReference type="EnsemblPlants" id="KQJ95348">
        <property type="protein sequence ID" value="KQJ95348"/>
        <property type="gene ID" value="BRADI_3g16680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842157"/>
    <dbReference type="Gramene" id="KQJ95348">
        <property type="protein sequence ID" value="KQJ95348"/>
        <property type="gene ID" value="BRADI_3g16680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842157"/>
    <dbReference type="eggNOG" id="KOG0439">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036554_1_2_1"/>
    <dbReference type="OMA" id="IYAPSNI"/>
    <dbReference type="OrthoDB" id="264603at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I1H2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000813">
        <property type="entry name" value="Vesicle-associated protein 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF150">
        <property type="entry name" value="OS08G0155100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019693">
        <property type="entry name" value="VAMP-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="5">
        <location>
            <begin position="10"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ95348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="240" mass="25900" checksum="09C593508101A01D" modified="2012-06-13" version="1">MSAGAGTGTFLEIQPSELAFPFELLKQSSCSMQLTNKTDHYVAFKVKTTNPKQYCVRPNIGVVLPGSTCDVTVTMQAPAMAPPDLQCKDKFLVQSVAAENGAATQDITAAMFNKEPGKVIDECKLRVLYVLTTAPGSNPEESELGSSSRSFTQENGTPNSTLLPSRSFGETGKEKSSEATTMISKLTEEKMSAIQQNQKLRQELDLLRKESSKSNGGFSLTFMIVVGLLGIVVGFILKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1I355</accession>
    <name>I1I355_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100825119</name>
        <name evidence="3" type="ORF">BRADI_3g21630v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573764.1">
        <property type="nucleotide sequence ID" value="XM_003573716.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I355"/>
    <dbReference type="FunCoup" id="I1I355">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I355"/>
    <dbReference type="EnsemblPlants" id="KQJ96222">
        <property type="protein sequence ID" value="KQJ96222"/>
        <property type="gene ID" value="BRADI_3g21630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825119"/>
    <dbReference type="Gramene" id="KQJ96222">
        <property type="protein sequence ID" value="KQJ96222"/>
        <property type="gene ID" value="BRADI_3g21630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825119"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100007_0_0_1"/>
    <dbReference type="OMA" id="WAGHANV"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24184:SF11">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24184">
        <property type="entry name" value="SI:CH211-189E2.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ96222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="261" mass="27737" checksum="F216BCFF3F283705" modified="2012-06-13" version="1">MGRRRAGGGGRGGGRGRGGGGEGRGRGRGEEEDLPLHKAARSGDAAAAESLCESDPLALNSRDRLSRTPLHLAAWAGHIEVVKCLCKHKADVGAAAMDDTAAIHFASQKGHMEVVRELLASGASVKAKNRKGFTALHFAAQNSHLELVKYLVKKGLDITAKTNGGQTALHVAENDDVRAFLKECEQALKKGEELPSEKKDDSVSEKSGDGKVSDEAVKDGAEAAQGEKRKSDDSGVPEQKKAKVSLRHLENDMEEEEEGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1I522</accession>
    <name>I1I522_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100832672</name>
        <name evidence="13" type="ORF">BRADI_3g29854v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Hakai family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574119.1">
        <property type="nucleotide sequence ID" value="XM_003574071.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I522"/>
    <dbReference type="FunCoup" id="I1I522">
        <property type="interactions" value="1159"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I522"/>
    <dbReference type="EnsemblPlants" id="KQJ97271">
        <property type="protein sequence ID" value="KQJ97271"/>
        <property type="gene ID" value="BRADI_3g29854v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832672"/>
    <dbReference type="Gramene" id="KQJ97271">
        <property type="protein sequence ID" value="KQJ97271"/>
        <property type="gene ID" value="BRADI_3g29854v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832672"/>
    <dbReference type="eggNOG" id="KOG2932">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041733_1_0_1"/>
    <dbReference type="OMA" id="HLPPKQH"/>
    <dbReference type="OrthoDB" id="547746at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16508">
        <property type="entry name" value="RING-HC_HAKAI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000280">
        <property type="entry name" value="E3 ubiquitin-protein ligase Hakai"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040380">
        <property type="entry name" value="HAKAI-like_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040383">
        <property type="entry name" value="HAKAI/CBLL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13480:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HAKAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13480">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HAKAI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="174"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="418"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ97271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="466" mass="50249" checksum="1A1E1EA9B07E6978" modified="2012-06-13" version="1">MLQIRLSKIGSSDSGAAASGAAAGAPGGAPAKSAAVAAGGVPESVTVACPDHLVIADLAVAKSLGAVTNSAIAASRAIGRRSRRPLGERVHICCRCEFPIAIYGRLNPCEHAFCLACARSDSSCYLCDERIQKIQTVKMMEGIFICAAPMCLKSFLKKAEFESHVPEVHANLLQTNADKEDRNEPDAPNISRASAGDTQRQSQMPEMSTARAPPRPGVSPTSSSHMQDREDRSRYHHSREQTPLRPPMLSKPPSFHGRHSYPPGDTQSENNPPQGFDRPYGWAHDSTPGATPLRQESDHGTQDKQQVMPNSPFMFSPMHPHQQNFMMHVNMNQPLMQNTPFNYPVQQDGNPQYFGSPFQMQPPDAGLDQGPVSGPEGLQRPWGMGLMGNPSQGGGGMVFMQAGFGIMPDNSMNPGMQGRDIQGQADRGDGRGVLEQLPMAMQMQMPLPPPPPAQPPSAGQQSFNRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5G2</accession>
    <name>I1I5G2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100834121</name>
        <name evidence="5" type="ORF">BRADI_3g31130v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574206.1">
        <property type="nucleotide sequence ID" value="XM_003574158.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5G2"/>
    <dbReference type="STRING" id="15368.I1I5G2"/>
    <dbReference type="EnsemblPlants" id="KQJ97483">
        <property type="protein sequence ID" value="KQJ97483"/>
        <property type="gene ID" value="BRADI_3g31130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834121"/>
    <dbReference type="Gramene" id="KQJ97483">
        <property type="protein sequence ID" value="KQJ97483"/>
        <property type="gene ID" value="BRADI_3g31130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834121"/>
    <dbReference type="eggNOG" id="KOG0743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010189_0_4_1"/>
    <dbReference type="OMA" id="EWRSAPF"/>
    <dbReference type="OrthoDB" id="10251412at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058017">
        <property type="entry name" value="At3g28540-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25568">
        <property type="entry name" value="AAA_lid_At3g28540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="227"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="420"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="492"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="421"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ97483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="520" mass="55978" checksum="E5CECBCA97864B40" modified="2012-06-13" version="1">MLLDGGGVAAAALYAVLAVVALRAVLSYKSAAHAARRLWRWADEWAQAYQYYEVPRFLGGGGDENPLFRKAAAYVSSLPSLEDADAACVLSSASKSNDFALQLGPGHTARDAFLGARLAWTNAGGGAAAGARERLVLRVRRHDRTRVLRPYLQHVESVADEMELRRRELRLHANTGAAAPRWASAPFTHPATLDTVAMDPELKTRIRADLETFLKGRAYYHRLGRVWRRSYLLYGPPGTGKSTFAAAMARFLGYDVYDVDLSRGGCDDDLRALLLDTAPRSLILVEDLDRYLRGGDGETSAARAARVLGFMDGLSSCCGEERVMVFTMSGGKEGVDPAVLRPGRLDVHIHFTMCDFEGFKALASNYLGLKDHKLYPQVEERFHAAGGARLSPAELGEIMLANRASPSRALRTVINALQHVSPPAQARTSSSSSAAPRPPRLSSRSSGNLGASSPAAESNAASQSPGSGGFGKDAPMREFKKLYGLIKIRSRKDGSGVVPVDDTASANGRGSDASSTEKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5Q6</accession>
    <name>I1I5Q6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Peroxidase</fullName>
            <ecNumber evidence="18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC100830524</name>
        <name evidence="21" type="ORF">BRADI_3g32130v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="18"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234947.2">
        <property type="nucleotide sequence ID" value="XM_010236645.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5Q6"/>
    <dbReference type="FunCoup" id="I1I5Q6">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I5Q6"/>
    <dbReference type="EnsemblPlants" id="KQJ97598">
        <property type="protein sequence ID" value="KQJ97598"/>
        <property type="gene ID" value="BRADI_3g32130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830524"/>
    <dbReference type="Gramene" id="KQJ97598">
        <property type="protein sequence ID" value="KQJ97598"/>
        <property type="gene ID" value="BRADI_3g32130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830524"/>
    <dbReference type="eggNOG" id="ENOG502QPX7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010543_0_3_1"/>
    <dbReference type="OMA" id="FIWISAN"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF84">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 18" id="KW-0349">Heme</keyword>
    <keyword evidence="12 18" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="8 15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="5 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 18" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982765" description="Peroxidase" evidence="18">
        <location>
            <begin position="25"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="25"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="35"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="68"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="120"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="199"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQJ97598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="320" mass="32777" checksum="719ABD37D570B0D1" modified="2012-06-13" version="1">MEAMRLLVQVAVTSALLMAAAVSAQLRVGFYDSSCPAAEIIVQQEVSSAVAANPGLAAGLLRLHFHDCFVGGCEASVLVDSTASNTAEKDAGPNKSLRGFEVIDRIKARVEQACFGVVSCADILAFAARDGIALTGGNGYQVPAGRRDGSVSKASDTSGNLPPPTPSVPQLTAIFASKGLTQKDMVTLSGAHTIGGSHCTSFSSRLQTPGPQTPDPTMDPGYVAQLASQCSSSSSGMVPMDAVTPNTFDEGYFKGVMANRGLLASDQALLGDGATAGQVVAYANDPATFQSDFAAAMVKMGYVGVLTGSSGKIRANCRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5Q9</accession>
    <name>I1I5Q9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100831140</name>
        <name evidence="9" type="ORF">BRADI_3g32160v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234948.1">
        <property type="nucleotide sequence ID" value="XM_010236646.3"/>
    </dbReference>
    <dbReference type="FunCoup" id="I1I5Q9">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I5Q9"/>
    <dbReference type="EnsemblPlants" id="KQJ97601">
        <property type="protein sequence ID" value="KQJ97601"/>
        <property type="gene ID" value="BRADI_3g32160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831140"/>
    <dbReference type="Gramene" id="KQJ97601">
        <property type="protein sequence ID" value="KQJ97601"/>
        <property type="gene ID" value="BRADI_3g32160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831140"/>
    <dbReference type="eggNOG" id="KOG1801">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005436_0_0_1"/>
    <dbReference type="OMA" id="NMITYDR"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I5Q9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045529">
        <property type="entry name" value="DUF6469"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF520">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20073">
        <property type="entry name" value="DUF6469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6469" evidence="8">
        <location>
            <begin position="121"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="6">
        <location>
            <begin position="353"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="7">
        <location>
            <begin position="724"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="243"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="967"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1106"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1148"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1215"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1153"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1261"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1323"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1369"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1402"/>
            <end position="1413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ97601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1490" mass="166740" checksum="6AF77FD2F7C1B887" modified="2012-06-13" version="1">MRRRNDAGDGDGDGDGAGDWRPEDDPAEPSCSLEGGGDSPVPMQASSGDSLWQWRSQGLSEVVFSWSVDQILNKDLLRDKVSKIPDTFHSMEQYMTSFFGPLLEEVRGDMCSSMEDISRAPFASVQSVDAMRKGKGLYEIKLDRWRGGSHGCGTEGYRPKAADVLLISETRPENQSDILKQSKSCVIIWVNKVQGNKMTVKASRRMETGAHGDERQQIGVNKYEKLYAEGFDKSWEMLDQEAMAPKSRNSSVNQKVWKEPPNDRKRSKNSTDPQEQNETGIYGNSSRRWSFYAMYLTNMITYDRVWIVLRRGLTMDSRIILNMLGKNNHAIRHCNYCSNKSYDEIKGGLCNFKLNESQLGAVASCISASNCFHRSSVRLIWGPPGTGKTTTLAVMLHMLLMKKQRILACAPTNMAVLQVASRLIGLIEDFSLKHHYSFGDIILFGNKDRLHIGKELSKIYLDDRVHKLLRCFKRENGWKHCVDSVLKFLTNCISRHRMSLDIQPAGSGECNLTFKKYFTSKFSSLAKELVACISTFSDHLPVDTLGKNFDKMMFLKSLLDKMQHLLCADDVSDELLFKIFKPSDKLPDPSTSHDDLADDATEGLPDLDISLDNPLEIKSMCIKILMDLSKMRLPCEDNESSIRDMCLKQAKLIFCTASGSFDLFRLQSVLPISILVIDEAAQLKESESLVPLLLPGIEHVLLIGDENQLSSLVKSKIAKDVDFGRSLYERLCAMDYNKHLLEVQYRMHPCISKFPNAKFYGNRISDSPSVKKKDYTKSYLPGPIYGSYSFIHIENDMEMLDDLGQSSKNMVEVAVAANIIERLAKECWKKSRRTSVGVISPYTAQVIALQEKLGRKFEKHEFLSVTVKSIDGFQGGEEDIILISTVRSNKDGKIGFLSDAGRINVALTRAKHCLWILGNGATLLASNSIWAELVNDSKRRGCFFEARKDKDLAETMRLVMKRNGRRIDATGEPSWPSRARHDVTGAGNSTPIRRSQLPPFGSGRGINNGHAWQQNGYDLRLKASHPNKPVFLAGREDRHGLHFEQHRTYSNQSRGVPANQYWPNRFGPYHDKNGDPQGFRGQVEQHLGEHYHSRTYQEPICSTSQTSNGRFAPGSVRRAGSHSQTSVLGEWQPSGGYCDREFQNRTAYPFGPVSSQRRFNSYGNADPHLSSMNKERQLSRHPQGAPYTYRERAPYRGHGGRGRENLSFHERAMRGDRDEQANNNQMEEPHCRVQNSSSEAASHDLPVPEQRGMKRNWCKAEASDSPRQDNTKARPSVKSAYQPNCQAHDGSPGAASHKLLVPKQPGMKREGCEAESSNPSHQDNTKVRPESADRPHNKAQDESSGVASLELPVPEQLRREGCEAEASYSPDQDNTKVSSESADQPDGQAHDGSSGAASHEPPVPDRPELKQEVCEAESSSSPHQDDTEASAQSLDRLHCQMQDTSSGAAPAPHQLHALEQEGCMETDLGDAEATVIPDINIRLESIEQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6C4</accession>
    <name>I1I6C4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 20">Pyruvate kinase</fullName>
            <ecNumber evidence="6 20">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC100830749</name>
        <name evidence="23" type="ORF">BRADI_3g33930v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 20">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 20">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="6 20"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574362.1">
        <property type="nucleotide sequence ID" value="XM_003574314.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6C4"/>
    <dbReference type="FunCoup" id="I1I6C4">
        <property type="interactions" value="352"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I6C4"/>
    <dbReference type="EnsemblPlants" id="KQJ97884">
        <property type="protein sequence ID" value="KQJ97884"/>
        <property type="gene ID" value="BRADI_3g33930v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830749"/>
    <dbReference type="Gramene" id="KQJ97884">
        <property type="protein sequence ID" value="KQJ97884"/>
        <property type="gene ID" value="BRADI_3g33930v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830749"/>
    <dbReference type="eggNOG" id="KOG2323">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_2_1"/>
    <dbReference type="OMA" id="SHMGAMF"/>
    <dbReference type="OrthoDB" id="108365at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="term" value="P:glycolytic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000003">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="17 20" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="12 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="21">
        <location>
            <begin position="85"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="22">
        <location>
            <begin position="457"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
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            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="566" mass="61199" checksum="E829F253E326026E" modified="2012-06-13" version="1">MAAAAAEMAVGFMATTTRAAAAAARPASAAAPPQPRRAVAARVLRTSASETVAADLATVGANGALSALSNSEVTADATSQNAVRRKTKIVCTIGPSTNTREMIWKLAETGMNVARMNMSHGDHQSHKKVIDLVKEYNAQNTDGNTIAIMLDTKGPEVRSGDVPEPIMLVEGQEFNFTIKRGVSTEDTVSVNYDDFISDVEAGDILLVDGGMMSLAVKSKTADTVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGVENGVDFYAVSFVKDAKVIHELKAYLKSANADIHVIPKIESADSIPNLQSIIAASDGAMVARGDLGAELPIEEVPLLQEEIIRTCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREAADAIMLSGETAHGKYPLKAVKVMHTVALRTESSLYDPTKAPSLVARPQALLNDDFCKSQLSKMFGSHATMMANTLRTPIIVFTRVGSMAVLLSHYRPSSTIYAFTNEVRVKQRLALYQGVVPILMDFSDDAEETFSRAISSLLDAKYMNEGDYVTLVQSGSKSIWRDESTHHIQVRKVQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8P4</accession>
    <name>I1I8P4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100833502</name>
        <name evidence="4" type="ORF">BRADI_3g40590v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ99020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003574740.4"/>
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        <property type="protein sequence ID" value="KQJ99020"/>
        <property type="gene ID" value="BRADI_3g40590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833502"/>
    <dbReference type="Gramene" id="KQJ99020">
        <property type="protein sequence ID" value="KQJ99020"/>
        <property type="gene ID" value="BRADI_3g40590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833502"/>
    <dbReference type="eggNOG" id="KOG1091">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014968_0_0_1"/>
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    <dbReference type="OrthoDB" id="27140at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:retromer complex"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000034">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000038">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF337">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="7"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="631"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="716"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="637"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="722"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="738"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ99020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="830" mass="91611" checksum="9E62948ADB37A890" modified="2012-06-13" version="1">MAEEERRRFSSLRSVRWRVDLGILPASPEASVEEVRRAAADSRRRYVSLRRRLLVDPHLPKEEARSSNLVVDNPLSQNPDSSWGRFFRGAELEKTVDQDLSRLYPEDGSYFQTPACQAMLRRILLMWCLQHPEYGYRQGMHELLAPLVYVLQVDVDKLSQVRKLHEDCFNDDFDGVPFPDTDMVFSYKPRKDPKWNSGADNENDSESASKVNTLDELDLDTKEIILLSDPYGAEGELGIVLSERFMEHDAYAMIDGLMDGGGGVVRMAEFFSPSSVGSSSSLPPAIEASSALYHLLSVVEPSLHSHFIELKVEPQWFALRWLRVLFGREFCLNDLLVVWDKVFACCNNMLLSSDQEYSFRILCSARGAFIAAMAVSMLLHVRSSLLATEVDVSCLQRLLNYPTNVDVQKLIEKAKSLQSIAIDANASSPSFLLRRDICEYDRVNSNLAISTPPRTQVQPLTESYWEEKWRNVHHDGTTPKEIEKGHSFSREIKKSLRQKLGLSRTESDPSPVKAVGVKSDARNSVRRCLLNTLSDSVGSSSEVAGKMEQDEFPVVSIHKEIPVSSAETLQLKATGETVTVSPPCLAKVSPLENSQTVPADDNATQRIQRATEACSSGETSPVFYAAIATNEIENGQDNDSSRSSVTSDSCDGDNDRDETLKDESSSCNCDGKTVRDSGATASDKTADPDGSSERSAVSNERKPFISKFQWLLKLGRPSGEGNIEKGSGETLDGRDGVGSFSSLPSDGNSNSSNGSTKLATGDKKVTGTFKNLGQSMLENIQVIESAFQQDRGQPGPMENFSNNILGGKGQATAMSALTELRKISNLLSEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8X8</accession>
    <name>I1I8X8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NTF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100846326</name>
        <name evidence="4" type="ORF">BRADI_3g41310v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235339.1">
        <property type="nucleotide sequence ID" value="XM_010237037.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8X8"/>
    <dbReference type="FunCoup" id="I1I8X8">
        <property type="interactions" value="2876"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I8X8"/>
    <dbReference type="EnsemblPlants" id="KQJ99134">
        <property type="protein sequence ID" value="KQJ99134"/>
        <property type="gene ID" value="BRADI_3g41310v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846326"/>
    <dbReference type="Gramene" id="KQJ99134">
        <property type="protein sequence ID" value="KQJ99134"/>
        <property type="gene ID" value="BRADI_3g41310v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846326"/>
    <dbReference type="eggNOG" id="KOG2104">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131642_0_0_1"/>
    <dbReference type="OMA" id="QFVEYYY"/>
    <dbReference type="OrthoDB" id="6507044at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000005">
        <property type="entry name" value="Nuclear transport factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="3">
        <location>
            <begin position="6"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ99134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="122" mass="13146" checksum="5ECF8AA8EC1D02DE" modified="2012-06-13" version="1">MDPDAVAKAFVQHYYQTFDANRGALVGLYQDGSMLTFEGDKFLGSAAIAGKLGSLPFQQCHHKIDTVDCQPSGPQGGVLVFVSGAITTGPGEHPLKFSQMFHLLPAGGSFYVQNDMFRLNYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IAZ8</accession>
    <name>I1IAZ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100843678</name>
        <name evidence="4" type="ORF">BRADI_3g47150v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318135.1">
        <property type="nucleotide sequence ID" value="XM_024462367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IAZ8"/>
    <dbReference type="EnsemblPlants" id="PNT68927">
        <property type="protein sequence ID" value="PNT68927"/>
        <property type="gene ID" value="BRADI_3g47150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843678"/>
    <dbReference type="Gramene" id="PNT68927">
        <property type="protein sequence ID" value="PNT68927"/>
        <property type="gene ID" value="BRADI_3g47150v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_49_2_1"/>
    <dbReference type="OMA" id="FCKSKRY"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051240">
        <property type="entry name" value="Mito_RNA-Proc/Resp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933:SF45">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="164"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="199"/>
            <end position="229"/>
        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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        <location>
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        </location>
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        <location>
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    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="771"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT68927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="821" mass="91723" checksum="F20CA29F1D6CDC39" modified="2012-06-13" version="1">MAPSPSSSGSPAAHLAHLNAILSAPNWHLHPSLPHLPALLAPSLPHTLNVPLPVRLAAATARAAAPCRHLLALSLPILFRLHSLSPPPLRGLFDRPFASLLNHLSRFGLNPLILRLFAYMHLHSPPAPTGATYNALIRSLCRRADLARALRYLSLMVRSGWCPDAYTFNSLIMGYCRTHQADAARDLFDKMPLRGFPQGVVCYTAMIEGLCEMARIDEALELFGEMEQPDAHTYGVLVKGLCEAGRGEEGLQMLQRMKKLGWRPSTRAYAAVINFWCRERKVTEAEEMLDEVSHKGLVPSVVTCTALVNAYCKEGRISDAVRVFEAMELRGCKPNVWTYNVLVCGFCKEGKVHSAMALLNRMRAHGVEPDVVTYNLLIRAQCLDGHIDSAFRLLRLMEGDDLAADMYTYNALVDALCKDGRIDQACSLFDSLEVRGKKPNLVTFNTLIDGLCKSGKVDIASSLFEKMVSAGYTPDTYTYSPFIENLCKMKGSQGGLSFIDEMLKKSVKATTVNYTIVIDKLFKEANYGLATKIWGQMVSLGCNADAVTYTTSMRAYCNEGKLSEAENVLTEMNKSGVTVDTIAYNTLMDGHVRIGQTDCAVSTLKHMKNTDSMPNQFTYMILLRHVLQRRLPESVSLNAASVWKTTELTDIFELFEVMKKNYIIPNTVTYSVILERFSEDGRLNEMTSLVSCMKDDKLSLNENIYTSLISCFCKSGRLSDAWALLHSMIEHGFVPRLMSYQHLLCGLISEGQDHRVKEIFGNSRWKDYNPDEIVWKVIIDGLIRKGHSDICLDMISKLEQMNCRPSNQTYAMLAEELSNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IB83</accession>
    <name>I1IB83_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Steroid nuclear receptor ligand-binding</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100827178</name>
        <name evidence="6" type="ORF">BRADI_3g47827v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572687.1">
        <property type="nucleotide sequence ID" value="XM_003572639.4"/>
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    <dbReference type="AlphaFoldDB" id="I1IB83"/>
    <dbReference type="STRING" id="15368.I1IB83"/>
    <dbReference type="EnsemblPlants" id="KQK00182">
        <property type="protein sequence ID" value="KQK00182"/>
        <property type="gene ID" value="BRADI_3g47827v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827178"/>
    <dbReference type="Gramene" id="KQK00182">
        <property type="protein sequence ID" value="KQK00182"/>
        <property type="gene ID" value="BRADI_3g47827v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827178"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="entry name" value="At3g27390-like"/>
    </dbReference>
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        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31133:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="40"/>
            <end position="66"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK00182.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="587" mass="64753" checksum="D4736317731AD50C" modified="2012-06-13" version="1">MEPSTGLWVSVWSCFKFLPFFCGLLLLGIIKGVLFGPWAWLIMTVGISAIILGLWPMHVVWTYYCIIRSKLVGPVVKLLLLISASGILVLWLIVGIVGSVIAGLVYGFLAPVMATFDAAGEGKERPLVHCLVDGTWSTITGSCTVVRDLKDMLFHSYFSIMDDLRFHAPPGGTPFEIRVLDIPGAVFAAACGLLTDGIMFTVIALYKLPVMLFKGWKRLIEDLVGREGPFLETACVPFAGLAILLWPFAVLGAFLASIISSVPLGVYAAIVVYQESSLLMGLFYVISSVSIFDEYTNDVLDMAQGSCFPRFAYRKNEVSTQSGPLSRPASFKDRQDEKKAPARMTSFKSSFDEFNPFKMLDHLFAECKHHGETLVSEGVITSKDIEETKSGKVGSGVINVGLPAYVILNALLRSVKANSDGLLLSDGCEITSDNRPKNTVFDWFFDPLMVIKDQIKAENFTEEEEAYLKKRVLLISDPKRLKATLPHLASLNERQQAEIDAFARRLQGITKSISRYPTSKRRFDDLVKALSEELERAMGGSQSVSGSQFQRLRSGVARMLSQRSMGKTAIIRGDDQEAQLTSNGRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBA2</accession>
    <name>I1IBA2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100835962</name>
        <name evidence="5" type="ORF">BRADI_3g47950v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575299.1">
        <property type="nucleotide sequence ID" value="XM_003575251.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBA2"/>
    <dbReference type="STRING" id="15368.I1IBA2"/>
    <dbReference type="EnsemblPlants" id="KQK00208">
        <property type="protein sequence ID" value="KQK00208"/>
        <property type="gene ID" value="BRADI_3g47950v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00209">
        <property type="protein sequence ID" value="KQK00209"/>
        <property type="gene ID" value="BRADI_3g47950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835962"/>
    <dbReference type="Gramene" id="KQK00208">
        <property type="protein sequence ID" value="KQK00208"/>
        <property type="gene ID" value="BRADI_3g47950v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00209">
        <property type="protein sequence ID" value="KQK00209"/>
        <property type="gene ID" value="BRADI_3g47950v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835962"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017106_0_0_1"/>
    <dbReference type="OMA" id="LLECECR"/>
    <dbReference type="OrthoDB" id="5314041at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IBA2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF38">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="227"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="588"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="590"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK00208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="709" mass="76328" checksum="90CC8620B135D1A0" modified="2012-06-13" version="1">MMKRSCYFPLRWESTGDQWWYASPIDWAAADGHYDIVRQLLHLDPNLLIKLTSLRRIRRLETLWDDDARFLDAPRHRASVARSLLLECECKHGAENTLLRAGYGGWLLYTAASAGDMGFVQELLDRDPLLVFGEGEYGVTDMFYAAARGGDAVVFRMLLDHAMSPRCSTNCRDGDGTNGGGGGGRASVFRLEMMSRAVHAAARGGSVQMLRELIDGSSDVSAYLDIRGSTVLHAAAGRGQLEVVKYLMASFDIINSTDNQGNTALHVAAYRGHLPVVQALVAASPSTLSAVNNAGETFLHSAIAGFRTPGFRRLDRQLELTKHLIQERTADIRKIINLKNDAGLTALHMAVVGCVHPDLVELLMTTPSIDLNVKDAGGMTPLSLLKEQLQSTTSERLIKQIVSAGGVLSSSVLRSRSAVVSQIKMRGGIAISPGTMFKISDAEIFLYSGIGTAGSRRASSCSSPGKCDLADANDEGGDVNHGSSGGKRLSSASRAKDRLKLMLRWPRHREKMLRTPRKPEDSGPLDSIKKLNEQVAAETPAPLRQAFTKTTALNNKRTLAVKSSAPSSATKKKLNMKLIHGIMEAMPQLASSSSANRSPPNTLPRSSMSSTPPSTKLKDICFDDEAPIMSGKLKDIVLDGNDGSVDDPSCSNSSFTGDDGVAGAARRNHGCGNGRLINICFGAQGLAVEDSASGQPTSKMFKQQCLRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBT8</accession>
    <name>I1IBT8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100845822</name>
        <name evidence="5" type="ORF">BRADI_3g49427v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575417.1">
        <property type="nucleotide sequence ID" value="XM_003575369.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756651.1">
        <property type="nucleotide sequence ID" value="XM_014901165.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBT8"/>
    <dbReference type="STRING" id="15368.I1IBT8"/>
    <dbReference type="EnsemblPlants" id="KQK00439">
        <property type="protein sequence ID" value="KQK00439"/>
        <property type="gene ID" value="BRADI_3g49427v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845822"/>
    <dbReference type="Gramene" id="KQK00439">
        <property type="protein sequence ID" value="KQK00439"/>
        <property type="gene ID" value="BRADI_3g49427v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845822"/>
    <dbReference type="eggNOG" id="KOG2051">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002633_2_0_1"/>
    <dbReference type="OMA" id="DFQHHQI"/>
    <dbReference type="OrthoDB" id="27832at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IBT8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000026">
        <property type="entry name" value="Regulator of nonsense transcripts UPF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000030">
        <property type="entry name" value="Regulator of nonsense transcripts UPF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000033">
        <property type="entry name" value="Regulator of nonsense transcripts UPF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="65"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="475"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="678"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="322"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="387"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="904"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1136"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="328"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="423"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="904"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="980"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1001"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1030"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1064"/>
            <end position="1074"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK00439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1195" mass="135424" checksum="E8FF08AAA27013FD" modified="2012-06-13" version="1">MENSQSENLADPKQDDEARQSKQEDEGARIEEYKRLMDQKTTLRRSNQNPERPDANYLRTLDSSIKRNTTVIKKLKTINDEQKDGLMDELKSVNLSKFVSEAVSYICEAKLRSADIQAAVQVCSLLHRRYKDFSPCLIQGLLKVFFPGKSGDDMDVDKNSRAMKKRSTLKLLIELYFVGIVEDASIFVNIIKDLTSLEHLKDREATQTNLSLLSTFVRQGRLFIGLQSHGHEPYDEFFKDLNVTADQKKFFKKALNSYYDAVSELLQSEHASLHLMEAENAKVLSAKGELSDENTASYEKLRKSFDQLLRGVSSLAEAIDLQPPVMPDDGNTTRVTTGTDVTPSSGKESSVLEPIWDDDDTKTFYESLPDLRAFVPAVLLGEAEQKLNEQHAKGREQSNESSAEQETEAHDNAQTSSATEDQLEGKTDDVAKDNEDKDKDKGKDPEKEKTKDKDLDRKTEKEKEKVRASDGGSLDNLLQRLPGCVSRDLIDQLTVEFCYLNSKASRKKLVRAVFSVPRTSLELLPYYSRLVATLSLCMKDVPSMLLSMLEEEFNFLINKKDQINIETKIKNIRFIGELCKFKIAPAALVFSCLKACLDDFSHHNIDVACNLLETCGRFLYRSPETTIRMANMLEILMRLKNVKNLDPRHSTLVENAYYLCKPPERSARVSKVRPPLYQYIRKLLFSDLDKSSVEHVLRQLRKLPWAECQQYLVKCFLKVHKGKYSQVHLIALLTAGLSRYHDDFAVAVVDEVLEEIRVGLELNDYAMQQRRLAHMRFLGELYSYKHIDSSVVFETLYLIILFGHGTQEQDVLDPPEDCFRIRLIITLLQTCGHYFTRGSSKRKLDKFLLHFQRYIIMKGPLPLDIEFDIQDLFVELRPNMTRYSSIDELNSALVELEEHERAVSAEKIESERHSDNESQKKQPHDAAFSVNGRGSVNGAEGNGREHGEAADSESYSDSGSIDGHEDEEDLLSDNKSNDASENEGDDEDDGIPVGSDEDEGVEVRQKVVQVDPKEQEDFDRELKALLQESLESRKSEVRTRSTLNMKVPMNVLEGSKDQRAGESESGEETMDEEGGNAGGGSKVRVKVLMKKGHKQQTRQMFIPGDCPLVQSTKEQEAAELEEKQSIKRRILEYNEREEEEMNGGSSQMGNWGQGGSSTGSSIRSGGRGNWDGWIRGGARRHHAAGGFYQGYGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBY1</accession>
    <name>I1IBY1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100823687</name>
        <name evidence="2" type="ORF">BRADI_3g49780v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572745.1">
        <property type="nucleotide sequence ID" value="XM_003572697.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBY1"/>
    <dbReference type="STRING" id="15368.I1IBY1"/>
    <dbReference type="EnsemblPlants" id="KQK00488">
        <property type="protein sequence ID" value="KQK00488"/>
        <property type="gene ID" value="BRADI_3g49780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823687"/>
    <dbReference type="Gramene" id="KQK00488">
        <property type="protein sequence ID" value="KQK00488"/>
        <property type="gene ID" value="BRADI_3g49780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823687"/>
    <dbReference type="eggNOG" id="ENOG502QUM1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028999_1_0_1"/>
    <dbReference type="OMA" id="YSRLYMN"/>
    <dbReference type="OrthoDB" id="1927466at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040412">
        <property type="entry name" value="At1g65710-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34367:SF3">
        <property type="entry name" value="DUF4005 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34367">
        <property type="entry name" value="OS02G0734667 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK00488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="599" mass="62762" checksum="1F7B19AE577E78BD" modified="2012-06-13" version="1">MGLCFSKKNQEKPPPPAKQPSAEASTKSKKKPGKVADEKAKKITPQPRRAAKAEEEPAAAAAGKKASVVVKSKAAAVVEEEKKKEEARQAVVVAKGPVPVRTSSCTKEEVDAILIQCGRLSRSSSGTGRAASSETGTGNRRRSGSKRSCDFDQERRGGGAEEDCDWEGHGVPVSRPSPHRSSPQRKRSGSRERTGGGSRRSSRSPGRRGEGGAPSAGAASSGAARQQPGKMVSVPAREKGRAPSPAVVAGKGCASPRSSSPARVAMAAGNENAGAGQKTAGPTPSLSRSSSRKAEKSPHRRNPMAEIDENSLRNNNSSNANPQKKSTEVAVAKPTERAKEKKAEIAEETMVAASETRAPSSKTTATRTATENLNPRASSRSRRPSRDFDQSPNLSATQVLDDIQNYHHASGTPPPSFSLPACVSKARSIVEAVADLNSSSSESRACERSNDKGSVNAPAGRHDDDVDLVEPSVHRYVSVRDIRGRGETELQESAGSNSLSGNPWTPSCESTDRTWSTSRSSNNGDEVVDQDPGRHGARSPMNRPRQSKQRPAAQPEPSGRSRAAGSSGVNAHRGRSSAHRGSGSVASGRSVARGVSAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICI2</accession>
    <name>I1ICI2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g51430v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICI2"/>
    <dbReference type="FunCoup" id="I1ICI2">
        <property type="interactions" value="1631"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1ICI2"/>
    <dbReference type="EnsemblPlants" id="KQK00726">
        <property type="protein sequence ID" value="KQK00726"/>
        <property type="gene ID" value="BRADI_3g51430v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00726">
        <property type="protein sequence ID" value="KQK00726"/>
        <property type="gene ID" value="BRADI_3g51430v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118311_0_0_1"/>
    <dbReference type="InParanoid" id="I1ICI2"/>
    <dbReference type="OMA" id="DWKNTVA"/>
    <dbReference type="OrthoDB" id="159299at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045238">
        <property type="entry name" value="Tim23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371:SF19">
        <property type="entry name" value="OS04G0566700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371">
        <property type="entry name" value="TIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK00726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="20786" checksum="D449D3D150C5E828" modified="2012-06-13" version="1">MSHDDAANIPAPGVDGRRQYVLDRPAFGRGAQLLYDLPTSPEFLFEEEALRKNRTWGENLTFYTGVGFCAGSLTGGARGLGRAIAAAERGESAKLRLSRALNECGSVGRKHGNRLGVIGLLFGGAESAVRHFRDDQDDWITTVSAGVGTGAIYGCPSGPRAAVVRGIVGGVLAGASTAVKPLLEKFAPDLAARFKLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1ID65</accession>
    <name>I1ID65_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100833274</name>
        <name evidence="12" type="ORF">BRADI_3g53247v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="11"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570227.1">
        <property type="nucleotide sequence ID" value="XM_003570179.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ID65"/>
    <dbReference type="STRING" id="15368.I1ID65"/>
    <dbReference type="EnsemblPlants" id="KQK01007">
        <property type="protein sequence ID" value="KQK01007"/>
        <property type="gene ID" value="BRADI_3g53247v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833274"/>
    <dbReference type="Gramene" id="KQK01007">
        <property type="protein sequence ID" value="KQK01007"/>
        <property type="gene ID" value="BRADI_3g53247v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833274"/>
    <dbReference type="eggNOG" id="KOG0831">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023995_2_0_1"/>
    <dbReference type="OMA" id="FWFTCAN"/>
    <dbReference type="OrthoDB" id="264532at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ID65">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF63">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK01007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="331" mass="36640" checksum="CC9C16CBA237F7F7" modified="2012-06-13" version="1">MGAGGNGLSNGGAGEAAADGTTVFKATAYSPLRTTVALALWLGGIHLNVVLVLASLFLLPRRIAAMVLGTQLFFMLVPVSDKSRLGRKIARFISKYVIGYFPVTLHVEDYNAFDPKRAYVFGYEPHSVLPIGLGALLDLVGFMPLPKIKVLASSAVFYTPFLRQIWTWLGLVPASRKSFYSYLGAGYTCIIVPGGVQEMLHMDHDSEVAFLKSRKGFVKIAMETGSPLVPVFCFGQSFVFKWWRPGGKLIVKIARAIKFTPIVFWGKFGTPIPFATPLHLVVGRPIEVKKNPQPNYDEINEVHEQFVVALQELFEKYKTKAGYDKLHLRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IIH1</accession>
    <name>I1IIH1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100822054</name>
        <name evidence="9" type="ORF">BRADI_4g07560v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 18 family. Xylanase inhibitor subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576258.1">
        <property type="nucleotide sequence ID" value="XM_003576210.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IIH1"/>
    <dbReference type="STRING" id="15368.I1IIH1"/>
    <dbReference type="EnsemblPlants" id="PNT62722">
        <property type="protein sequence ID" value="PNT62722"/>
        <property type="gene ID" value="BRADI_4g07560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822054"/>
    <dbReference type="Gramene" id="PNT62722">
        <property type="protein sequence ID" value="PNT62722"/>
        <property type="gene ID" value="BRADI_4g07560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822054"/>
    <dbReference type="eggNOG" id="KOG4701">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007818_0_0_1"/>
    <dbReference type="OMA" id="WEDAWDR"/>
    <dbReference type="OrthoDB" id="663593at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000044">
        <property type="entry name" value="Chitinase III C10701-rice"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF23">
        <property type="entry name" value="GH18 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003644853" description="GH18 domain-containing protein" evidence="7">
        <location>
            <begin position="29"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="8">
        <location>
            <begin position="33"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT62722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="306" mass="33603" checksum="98520C0C7699A5BE" modified="2012-06-13" version="1">MAMPRPILILAALLCVVQISFLARRAAGTGKTSQLTVFWGRHKDEGSLREACDSGMYTAVIVSFLNIVSGHDNAKYNYNLDLSGHPLAGIGDDIKHCQITGVPVSLSLRGAGALPTNQSALHLSDHLWFSYLSGFQKGVRRPFGDAKLDGVDFFLDHGKEEEEYYGVLAKDLQAKRRQSPAGTTKPLQLTATPGCALLTAGRALAAAGITLERIHVRFYGDASCSGGAWVEDAWGKWAAAYRRPGSRIYLGLTASGKTEDGYLYPKELYYGVIPEVQKAANYGGVMLWDRYYDKRNEYSSYVKYWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJC7</accession>
    <name>I1IJC7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_4g10030v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJC7"/>
    <dbReference type="FunCoup" id="I1IJC7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ87256">
        <property type="protein sequence ID" value="KQJ87256"/>
        <property type="gene ID" value="BRADI_4g10030v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87256">
        <property type="protein sequence ID" value="KQJ87256"/>
        <property type="gene ID" value="BRADI_4g10030v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000837_8_6_1"/>
    <dbReference type="InParanoid" id="I1IJC7"/>
    <dbReference type="OMA" id="IQCFIND"/>
    <dbReference type="OrthoDB" id="3027644at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF909">
        <property type="entry name" value="OS11G0673900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="190"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="427"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="562"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ87256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1012" mass="113904" checksum="A80F2F6349FD3428" modified="2012-06-13" version="1">MASVLDPLVGSCITKLQDIIAEKAVLILDVKEELEKMQGTMRQIRCFLDDAEQRRIKESAVNNWLSELRDAMYDAVDIVDSARFEGSKLLKDRKSSSSKNSTAGCGISLLSCFPVIQRRHEIAVKIRDLNDRVEQLSKHGNSFLHPGVGPTGQGSTSKGRENSNLVQPKLVGKEIMHSSKKLVDLVLAGKEQKDYRLAIVGTGGVGKTTLAQKIYNEQKIKPVFEKQAWVCVSQECNEVNLLKEILRNIGVYQDQGETIAELQRKIAKTIEGKSFFLVLDDVWKSSVIDLIEAPIYAAASSVILVTTRDDRIAMDIHAAHTHRVNLLSEEVGWELLWKSMNIDEEKEVQNLRNTGIQIIKKCGYLPLAIKVIARVLTSKDQTENEWKKILSKISAWSELHDDIEGALYLSYNELPHHLKQCFLYCALYPEDSTIKRDDLVMLWVAEGFIEEQEGQLLEETGEEYYYELIHRNLLQPDGSTFDHTNCKMHDLLRQLACYLSRDECFTGDPESLEGQSMTKLRRISAVTKKDMLVFPTMDKEHLKVRTLLRKFYGVSQGVDHSLFKKLLLLRVLDLTGSSIQTIPDCIANLIHLRLLDLNGTEISCLPEVMGSLINLQILNLQRCDALHNLPSSITQLCNLRRLGLEDTPINQVPEGIGRLTFLNDLEGFPIGGGSDIGKTQDGWKLEELGHLLQLRRLHMIKLERASPPTTDSLLVDKKYLKLLSLNCTKHPVESYSEGDVGNIEKIFEQLIPPHNLEDLIIADFFGRRFPTWLGTTHLVSVKHLILIDCNSCVHLPPLWQLPNLKYLRIDGAAAVTKIGPEFVGCRGDNPRSTVAAAFPKLETLVIEDMPNWEEWSFVEEGDAAAASMEGEEDGSAEIRKGEAPSPRVQVLPRLKWLRLDGCPKLRALPRQLGQEATCLEELGLRGASSLKVVEDLPFLSEALICGCDGLERVSNLPVLRELYAQDCPHLRCVDGLGNLQQLWLDDDMQEVSKLWVPGLQQIRGEDLDVYTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1INJ4</accession>
    <name>I1INJ4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100822988</name>
        <name evidence="8" type="ORF">BRADI_4g25717v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237946.1">
        <property type="nucleotide sequence ID" value="XM_010239644.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INJ4"/>
    <dbReference type="STRING" id="15368.I1INJ4"/>
    <dbReference type="EnsemblPlants" id="KQJ89441">
        <property type="protein sequence ID" value="KQJ89441"/>
        <property type="gene ID" value="BRADI_4g25717v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822988"/>
    <dbReference type="Gramene" id="KQJ89441">
        <property type="protein sequence ID" value="KQJ89441"/>
        <property type="gene ID" value="BRADI_4g25717v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822988"/>
    <dbReference type="eggNOG" id="ENOG502STKS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087453_0_0_1"/>
    <dbReference type="OMA" id="SYRANQQ"/>
    <dbReference type="OrthoDB" id="770297at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1INJ4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31282">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="45"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ89441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="239" mass="25665" checksum="8D5A265F1C1AA50F" modified="2012-06-13" version="1">MALDSVPAYPGDLGSSTTRASGSGGGGGGRTQHRIRKEERTWTADTYAPYDDGHQWRKYGEKKLSNSNFPRFYYRCTYKNDMKCPATKQVQQKDTSDPPLFSVTYFNHHTCNTTSRPIGSAPDTMEQPSSRKAVSICFGSSAATEQLPAFLTPPSTAALHSPASRGNDPQDRGTYAPQFQWADASSSLSTGNAPVKMEADDHAGGAGSSSSSITGGGGALSRTLLPIGQSRCIEYFQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1INV0</accession>
    <name>I1INV0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="10">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100843603</name>
        <name evidence="12" type="ORF">BRADI_4g26610v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(E)-4-coumarate + ATP + CoA = (E)-4-coumaroyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19641"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19642"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(E)-4-coumarate + ATP + H(+) = (E)-4-coumaroyl-AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:72419"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:72420"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(E)-4-coumaroyl-AMP + CoA = (E)-4-coumaroyl-CoA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:72423"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:72424"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="10"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577890.1">
        <property type="nucleotide sequence ID" value="XM_003577842.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INV0"/>
    <dbReference type="STRING" id="15368.I1INV0"/>
    <dbReference type="EnsemblPlants" id="KQJ89589">
        <property type="protein sequence ID" value="KQJ89589"/>
        <property type="gene ID" value="BRADI_4g26610v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843603"/>
    <dbReference type="Gramene" id="KQJ89589">
        <property type="protein sequence ID" value="KQJ89589"/>
        <property type="gene ID" value="BRADI_4g26610v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843603"/>
    <dbReference type="eggNOG" id="KOG1256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_45_4_1"/>
    <dbReference type="OMA" id="GQLCKDP"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016207">
        <property type="term" value="F:4-coumarate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106290">
        <property type="term" value="F:trans-cinnamate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF95">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="11">
        <location>
            <begin position="110"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ89589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="697" mass="76552" checksum="EC5DBDF8BF7159E7" modified="2012-06-13" version="1">MDASDRRLRAVSAHLRPPATFAPLRSGSDLAASPTAGEYAHVLGYSVVLPEKLQTGKWNVYRSAQSPITLVNKPLDNPDIGTLHDNFVYAVETFRDCTYLGTRVQADGTVGDYKWMTYGEASTSRTAIGSGLIYHGIPEGASVGLYFINRPEWIIADHACSAYSYVSVPLYDTLGPDAVQFIVNHATVEAIFCVPQTLSTLLSFITQMPCVRLIVVVGGIDENMPSASAITGVEIITYSRLHNQGHTSPQPFRPPKPEDVATICYTSGTTGTPKGAVLSHANLIANVAGSSLVIKFYPSDVYISYLPLAHIYERVNQIWLLQRGVAIGFYQGDNLKLMDDLATLRPTIFASVPRLYNRIYAAITNAVKESGGMKERLFNVAYHAKRQAIINGRNPSPMWDKLVFNKIKARLGGRVRLMSSGASPLSPDVMEFLRICFGGEILEGYGMTETSCVISTMDVGDKSIGHVGSPNPACEVKLVDVPEMNYTSEDQPYPRGEICVRGPTIFLGYYKDEIQTREVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIENVYAKCKYIAQCFVYGDSLNSSLVAIIAVEPDVLKAWAVSEGIQCEDLRQLCADPRAKAAVLADMDSIGKEAQLRGFEFVKAVALIAEPFTLENGLLTPTFKVKRPQAKAYFAKEISDMYAQLHDAESGRAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPE1</accession>
    <name>I1IPE1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC100835757</name>
        <name evidence="21" type="ORF">BRADI_4g28107v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576419.1">
        <property type="nucleotide sequence ID" value="XM_003576371.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPE1"/>
    <dbReference type="FunCoup" id="I1IPE1">
        <property type="interactions" value="718"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IPE1"/>
    <dbReference type="EnsemblPlants" id="KQJ89848">
        <property type="protein sequence ID" value="KQJ89848"/>
        <property type="gene ID" value="BRADI_4g28107v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835757"/>
    <dbReference type="Gramene" id="KQJ89848">
        <property type="protein sequence ID" value="KQJ89848"/>
        <property type="gene ID" value="BRADI_4g28107v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835757"/>
    <dbReference type="eggNOG" id="ENOG502QQFB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_22_1_1"/>
    <dbReference type="OMA" id="TDQFRPS"/>
    <dbReference type="OrthoDB" id="2021138at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000453">
        <property type="entry name" value="Leucine-rich receptor-like protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000201">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000228">
        <property type="entry name" value="Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000215">
        <property type="entry name" value="Receptor-like protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF70">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 19" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095395" description="non-specific serine/threonine protein kinase" evidence="19">
        <location>
            <begin position="19"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="603"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="661"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KQJ89848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="950" mass="102737" checksum="FB7F4D88686B966E" modified="2012-06-13" version="1">MAALFLALLLLSLRTAAPYDAGSLLAAKRKLSDPAGALSGWKARSGGHSPCAWPHVACAVNSTTDVAGLYLKNVSLSGVFPASLCSLRSLRHLDLSQNDIGGPLPVCLAALPALAYLDLSGNNFSGHVPAAYGAGFRSLATLNLVENALSGAFPAFLANLTSLQELMLGYNDFTPSPLPENLGDLAGLRLLYLSRCYLKGRIPSSLGNLRNLVNLDMSVNGLSGEIPGSIGNLGSAVQIEFYSNQLSGRIPEGLGRLKKLQFLDLSMNLLSGAMPEDAFAGPRLESVHIYQNNLSGRLPASLASAPRLNDLRLFGNQIEGPFPPEFGKNTPLQFLDMSDNRLSGPIPPTLCASGRLAEIMLLNNKLEGSIPVELGQCWSLTRIRLLNNSLSGTVPPEFWALPNVRMLELRLNALSGTIDPAIGGARNLSKLLLQDNRFTGALPAELGNLAILKELFVSGNNLSGPLPASLVELSELYTIDLSNNSLSGEIPRDIGRLKKLVQVRLSHNHLTGVIPPELGEIDGISVLDLSHNELSGGVPGQLQKLRIGNLNLSYNKLTGPLPDLFTNGAWYNNSFLGNPGLCNRTCPSNGSSDAARRARIQSVASILAVSAVILLIGFTWFGYKYSSYKRRAAEIDRENSRWVFTSFHKVEFDEKDIVNSLDEKNVIGEGAAGKVYKAVVGRRSELALAVKKLWPSNTVSTKMDTFEAEVATLSKVRHRNIVKLFCSMANSTCRLLIYEYMPNGSLGDFLHSAKAGILDWPTRFKIAVHAAEGLSYLHHDCVPSILHRDVKSNNILLDADFGAKVADFGVAKAIVDGTATMSVVAGSCGYIAPEYAYTIHVTEKSDVYSFGVVILELVTGKWPMASEIGEKDLVAWVRDTVEQNGVESVLDQKLDSLFKDEMHKVLHIGLMCVNIVPNNRPPMRSVVKMLLDVEEENKRKARIEASLPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1IV21</accession>
    <name>I1IV21_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pulmonary surfactant-associated protein B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Pulmonary surfactant-associated proteolipid SPL(Phe)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100830063</name>
        <name evidence="14" type="ORF">BRADI_4g44500v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579028.1">
        <property type="nucleotide sequence ID" value="XM_003578980.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IV21"/>
    <dbReference type="STRING" id="15368.I1IV21"/>
    <dbReference type="EnsemblPlants" id="KQJ92568">
        <property type="protein sequence ID" value="KQJ92568"/>
        <property type="gene ID" value="BRADI_4g44500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830063"/>
    <dbReference type="Gramene" id="KQJ92568">
        <property type="protein sequence ID" value="KQJ92568"/>
        <property type="gene ID" value="BRADI_4g44500v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830063"/>
    <dbReference type="HOGENOM" id="CLU_077249_0_0_1"/>
    <dbReference type="OrthoDB" id="69496at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IV21">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.225.10:FF:000008">
        <property type="entry name" value="Pulmonary surfactant-associated protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF100">
        <property type="entry name" value="SAPOSIN B-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095535" description="Pulmonary surfactant-associated protein B" evidence="12">
        <location>
            <begin position="23"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="13">
        <location>
            <begin position="66"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="13">
        <location>
            <begin position="152"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ92568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="248" mass="27604" checksum="0AA84C151347CE4F" modified="2012-06-13" version="1">MNSRLGAPVFLLLLLLLAVGSGAVLQDRRLLRDRALYLDTSEVLDSRPIYDEQIATTKIPVPVERGCPLCSTCEMFTNKSVSYLSQKETQDDILEILHGACSQTFSLAEKCVEFVDSYASLLFAKVAEIKPDEFCKQYGLCRDAAILSAAKSESTCAFCHQLIDEVLSKMKDPDAQFEIIQLLIKECNKVQGHVQQCKRMVLQYVPLIVANGEKFLKKKDVCTLMQACDASQKRAVASFFDSGLRSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IYH4</accession>
    <name>I1IYH4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ArsA/GET3 Anion-transporting ATPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100822194</name>
        <name evidence="8" type="ORF">BRADI_5g12380v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239988.1">
        <property type="nucleotide sequence ID" value="XM_010241686.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IYH4"/>
    <dbReference type="STRING" id="15368.I1IYH4"/>
    <dbReference type="EnsemblPlants" id="KQJ82974">
        <property type="protein sequence ID" value="KQJ82974"/>
        <property type="gene ID" value="BRADI_5g12380v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822194"/>
    <dbReference type="Gramene" id="KQJ82974">
        <property type="protein sequence ID" value="KQJ82974"/>
        <property type="gene ID" value="BRADI_5g12380v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822194"/>
    <dbReference type="eggNOG" id="KOG2825">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_2_1_1"/>
    <dbReference type="OMA" id="WSTRERY"/>
    <dbReference type="OrthoDB" id="608491at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IYH4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000936">
        <property type="entry name" value="Arsenical pump-driving ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF8">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="7">
        <location>
            <begin position="61"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ82974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="371" mass="39916" checksum="20C403DB6ECEB7F8" modified="2012-06-13" version="1">MAALLNPTARHLALAACRRLSTPPAAEPLVAHWSTQARYSSSLVEINGGFKEMVTGTQSYYVVGGKGGVGKTSIAASLAMKLAKHGQPTLIVSMLPAHSLGDTFEQDLSGNKIVPVNGVDSLFAAEIGHVNIKEESSNAGSFISDMLGKIGLGVLADPLGSNKLNEMLMKTPGFGEALAMSKLAEIVNLQQNNKFRQIVLDTAATGHTLNLLSATDLMEKFLGMANKAVNLASSIPALKSAFEKGKIDFARIEELRKQIARVHKLLQDPQSTEFIIVTIPTAMAITESSRFHTSLKKDGAPAKRLIVNQVLPPSACDCRFCAVKRKEETRALNMISEDRELSGLELIQAPLLDVEVRGVPALNFFSDVVWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZ91</accession>
    <name>I1IZ91_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100844524</name>
        <name evidence="5" type="ORF">BRADI_5g14530v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580060.1">
        <property type="nucleotide sequence ID" value="XM_003580012.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZ91"/>
    <dbReference type="STRING" id="15368.I1IZ91"/>
    <dbReference type="EnsemblPlants" id="KQJ83369">
        <property type="protein sequence ID" value="KQJ83369"/>
        <property type="gene ID" value="BRADI_5g14530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844524"/>
    <dbReference type="Gramene" id="KQJ83369">
        <property type="protein sequence ID" value="KQJ83369"/>
        <property type="gene ID" value="BRADI_5g14530v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844524"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_9_0_1"/>
    <dbReference type="OMA" id="MAGDYGS"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000387">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490">
        <property type="entry name" value="(+)-NEOMENTHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490:SF60">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ83369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="298" mass="32342" checksum="EEB96BACEC4CC0B8" modified="2012-06-13" version="1">MDYSGSSSSSKEPSPTGAWWSKETVAVVTGANRGIGLALAARLAEQGLTVVLTARDGVRGEAAAAPLRDRGLPVVFRRLDVSDAASVAGFAGWLRNAVGGLDILVNNAAVSFNEIDTNSVEHAETVLRTNFYGAKLLTEALLPLFRRSPATSRILNISSQLGLLNKVSNPSLMRLLQDEETLTEAKIEGMVSQFLAQVKDGTWAEHGWPKVWTDYSVSKLALNAYTRVLARRLRERGERVSANCFCPGFTRTDMTKGWGKRTAEEVADVGARLALLPPGELPTGTFFKWCTPQLYSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0A2</accession>
    <name>I1J0A2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100832404</name>
        <name evidence="8" type="ORF">BRADI_5g17480v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580272.1">
        <property type="nucleotide sequence ID" value="XM_003580224.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0A2"/>
    <dbReference type="STRING" id="15368.I1J0A2"/>
    <dbReference type="EnsemblPlants" id="KQJ83906">
        <property type="protein sequence ID" value="KQJ83906"/>
        <property type="gene ID" value="BRADI_5g17480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832404"/>
    <dbReference type="Gramene" id="KQJ83906">
        <property type="protein sequence ID" value="KQJ83906"/>
        <property type="gene ID" value="BRADI_5g17480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832404"/>
    <dbReference type="eggNOG" id="ENOG502QRIC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058713_3_0_1"/>
    <dbReference type="OMA" id="PANICRN"/>
    <dbReference type="OrthoDB" id="1647183at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF287">
        <property type="entry name" value="DEVELOPMENT RELATED ERF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="147"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="226"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ83906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="289" mass="30566" checksum="87B792F6B5CF5278" modified="2012-06-13" version="1">MLLNPAASEALVLDSIRQHLMEETHGAAAPSQGQKGGAMTRPAYLRSASFGSLVADQWSSSLPFRADDSDDMVVYGALRDAFSCGWLPDGSFAAVKPEPLPSSPDSYAIADASSSSCFAFLDPDTPSEEAMPAPTRSEEASARGKQHYRGVRMRPWGKFAAEIRDPAKNGARVWLGTYDAAEDAALAYDRAAYRMRGSRALLNFPLRITSEIAAAAAAGLKRPAALEPATSNSSSSSSSSSGSPKRRKRGEAAAASMAMPLVPPPSSQAWFAPVEQVVGMAPRLEQLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0Z7</accession>
    <name>I1J0Z7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100821787</name>
        <name evidence="22" type="ORF">BRADI_5g19480v3</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581569.1">
        <property type="nucleotide sequence ID" value="XM_003581521.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0Z7"/>
    <dbReference type="EnsemblPlants" id="KQJ84223">
        <property type="protein sequence ID" value="KQJ84223"/>
        <property type="gene ID" value="BRADI_5g19480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821787"/>
    <dbReference type="Gramene" id="KQJ84223">
        <property type="protein sequence ID" value="KQJ84223"/>
        <property type="gene ID" value="BRADI_5g19480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821787"/>
    <dbReference type="eggNOG" id="ENOG502QSBD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_62_6_1"/>
    <dbReference type="OMA" id="RMGMEVA"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000626">
        <property type="entry name" value="probable L-type lectin-domain containing receptor kinase S.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000198">
        <property type="entry name" value="Probable L-type lectin-domain containing receptor kinase S.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000749">
        <property type="entry name" value="Putative L-type lectin-domain containing receptor kinase S.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019825">
        <property type="entry name" value="Lectin_legB_Mn/Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00307">
        <property type="entry name" value="LECTIN_LEGUME_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095693" description="non-specific serine/threonine protein kinase" evidence="20">
        <location>
            <begin position="25"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="334"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KQJ84223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="704" mass="77598" checksum="D18DE84F6FAFCC27" modified="2012-06-13" version="1">MTAVHNNIIRCSTFLVVLCTACSSLQFTYPSFDDTTNESDFSFTPGTAISNGSLHITPNTGNLRHRSGRVVYARETLKLWNSNRTALTSFRTEFTLNILPLERNGTGEGMAFFLTNNPSLPRDSSGQWLGLSNKQTDGAAANRIVAVEFDTRRSVEAGDIDGNHFGVDNNGVKLMWQYPLSNLSIVLASGVDVWVGLTYDGATVSVQAVQEGRTFAYSWLADLSPYLLDDDIFFGFSASNGESPQLNQIRSWNFSTYGEAIAGADDDEGRYRRQSLILAVSIPLAMFALFVALLVWRRLTRQTRLAYRNLEKMIDAHGPVRFKLRELRKATAGFSTARKLGRGRSGTVYLGYLSRMSMEVAVKRVSTNVNSNRGEKEFVAEVNTISKLSHRNLVKLIGWCHNKGELLLVYEYFPMGSLDKLLYARPSSRAAATPPSSTTTGQTNTSASDPELTWERRYRIIRGVASALDYLHHGSSKRILHRDVKASNVMLDEEYNARLGDFGLARVISHDGVTHHSTQAVAGTRGYMAYESFFTGRASLDTDVFAFGVFVMEVVSGKSPCSSVLCHSDDSRGESRAGSHSRPAPMYIVDWTWRLYGEGKALHVADEVLGGMYDEAQVDRAVRLALACCHPNPRERPSMRTAVQVLIDGAPAPEPPFDKPAFVWPPGGNQQEMELPDVGLLFTGGAGQHRHSSFGSISCSISGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>I1J310</accession>
    <name>I1J310_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100824752</name>
        <name evidence="13" type="ORF">BRADI_5g25157v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the ARF family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579438.1">
        <property type="nucleotide sequence ID" value="XM_003579390.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J310"/>
    <dbReference type="SMR" id="I1J310"/>
    <dbReference type="STRING" id="15368.I1J310"/>
    <dbReference type="EnsemblPlants" id="KQJ85149">
        <property type="protein sequence ID" value="KQJ85149"/>
        <property type="gene ID" value="BRADI_5g25157v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824752"/>
    <dbReference type="Gramene" id="KQJ85149">
        <property type="protein sequence ID" value="KQJ85149"/>
        <property type="gene ID" value="BRADI_5g25157v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824752"/>
    <dbReference type="eggNOG" id="ENOG502QQ0Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002626_2_0_1"/>
    <dbReference type="OMA" id="LKPNKHP"/>
    <dbReference type="OrthoDB" id="2016915at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J310">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010305">
        <property type="term" value="P:leaf vascular tissue pattern formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009942">
        <property type="term" value="P:longitudinal axis specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048507">
        <property type="term" value="P:meristem development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000047">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF10">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="11">
        <location>
            <begin position="144"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="851"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="534"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="629"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="539"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="559"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="569"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="636"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ85149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="955" mass="105663" checksum="D80A572B58B09A37" modified="2012-06-13" version="1">MASSQEKQPTFGVLRNAAALLDEMQLLGEAQGAKKVINSELWHACAGPLVFLPQRGSLVYYFPQGHSEQVAATTRKVPNSRIPNYPNLPSQLLCQVHNITMHADKETDEVYAQMTLQPVNSETDVFPIPALGSYAKSKHPPEYFCKNLTASDTSTHGGFSVPRRAAEKLFPQLDYSMQPPNQELIVRDLHDNMWTFRHIYRGQPKRHLLTTGWSLFVGAKRLKAGDSVLFIRDEKSQLLLGVRRATRQQTQLSSSVLSTDSMHIGVLAAAAHAASSGSSFTIYYNPRTSPSPFVVPLARYNKANYVQQSVGMRFAMMFETEESSKRRYTGTIVGVSDYDPIRWPNSKWRNLQVEWDEHGYGERPERVSIWDIETPENALVFPSSSLNSKRQCLPGYGVPGLEIGSVNMPSLTEIPTVLGNPRALGNPYGNLQHMPAVGSELAMMLLSQSGQNLGTPLSCHQSSYSSIIQNVKQNYVPPSTFGVSTVPIKQESMHSNEAQQQLHAPKLRRGDLENCELQPVTDSVSASELNVAVREPRNTDSYQSQSISEQNGKGEPKVKTRRSKKGLSRKTISESSELSSTPSRVCADQQHGFEVKLVAGDTQQINSANNEDSSGALAHGNFARQLQGQQVEQNGSLSPPKLESSKSPDGGKSVNSFPNQGCFSQFFDGLDWMIQPSYYQDSNGIQSVSASENIFSSSANIPSTINADTMEAFQNSCLSDCFPNSIQDFIGSPDLNSITFLSPDMQNLDVQHDGSNLPSTSNSYVQMSFSEDSGNHMESIQRSMNNTSCSQPQATESFDLGMYSKLPSLKETQVLSLPEIHNSSRGTSSCSMDVTDYSIDRSAKPLKPPVRTYTKVQKLGSVGRSIDVTRYRDYRELRSAIASMFGLQGKLEHPGSSEWKLVYVDYENDVLLVGDDPWEEFINCVRCIRILSPSEVQQMSESGMHVLNDCIQTDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1J3B3</accession>
    <name>I1J3B3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">dolichyl-diphosphooligosaccharide--protein glycotransferase</fullName>
            <ecNumber evidence="7">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC100821674</name>
        <name evidence="23" type="ORF">BRADI_5g26030v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="7"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580816.1">
        <property type="nucleotide sequence ID" value="XM_003580768.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J3B3"/>
    <dbReference type="FunCoup" id="I1J3B3">
        <property type="interactions" value="3320"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J3B3"/>
    <dbReference type="EnsemblPlants" id="KQJ85269">
        <property type="protein sequence ID" value="KQJ85269"/>
        <property type="gene ID" value="BRADI_5g26030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821674"/>
    <dbReference type="Gramene" id="KQJ85269">
        <property type="protein sequence ID" value="KQJ85269"/>
        <property type="gene ID" value="BRADI_5g26030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821674"/>
    <dbReference type="eggNOG" id="KOG2292">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009279_1_0_1"/>
    <dbReference type="OMA" id="TWYAIGT"/>
    <dbReference type="OrthoDB" id="10261066at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12610:FF:000002">
        <property type="entry name" value="dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048999">
        <property type="entry name" value="STT3-PglB_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21436">
        <property type="entry name" value="STT3-PglB_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="310"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="398"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="21">
        <location>
            <begin position="33"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="STT3/PglB/AglB core" evidence="22">
        <location>
            <begin position="547"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00059243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF21436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KQJ85269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="723" mass="81058" checksum="99D133FDD1678143" modified="2012-06-13" version="1">MAAASAATTSALEPLLRSLRLKTKQQELLLRVSALALIYVLAFAVRLFSVLRYESMIHEFDPYFNYRTTLYLTEHGYGEFWNWFDSESWYPLGRVVGGTLFPGLMVTAALLHRLLHALSLAVHIREVCVLTAPFFAANTTLVAYAFGREIWDSGAGLVAAALIAVCPGYISRSVAGSYDNEGVAIFALLLTFYLFVRAVNTGSLAWALASAFGYFYMVSAWGGYVFIINLLPLYVLVLLVTGRYSQRLYVAYNCMYILGMLLGMQIRFVGFQHVQSGEHMAAMGVFFLLQVFFFLDWVKYMLNDVKLFKSFLRITLTCVITVGTLALGIGTASGYISPWTGRFYSLLDPTYAKDHIPIIASVSEHQPTAWSSFMFDFHILLFLFPAGLYFCFKRLSDATIFIVMYGLTSMYFAGVMVRLILVAAPAVCLISAIAVSATMKNLTTLIRTKSKSPQTTSGKATGSKAAAKGAVDQPLPFQHNVAIALLLGAFYLLSRYAIHCTWVTSEAYSSPSIVLSARGHNGGRVIFDDYREAYYWLRQNTPTDAKIMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMQSLDVNYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRVDKGASHKMLNCLMYKLCYYRFGELTTEYGKPPGYDRVRGVEIGNKDVKLEYLEEAFTTSNWIVRIYKVKPPKNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1J3D5</accession>
    <name>I1J3D5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fiber protein Fb34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100824562</name>
        <name evidence="9" type="ORF">BRADI_5g26217v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DESIGUAL family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580825.1">
        <property type="nucleotide sequence ID" value="XM_003580777.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J3D5"/>
    <dbReference type="FunCoup" id="I1J3D5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J3D5"/>
    <dbReference type="EnsemblPlants" id="KQJ85302">
        <property type="protein sequence ID" value="KQJ85302"/>
        <property type="gene ID" value="BRADI_5g26217v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824562"/>
    <dbReference type="Gramene" id="KQJ85302">
        <property type="protein sequence ID" value="KQJ85302"/>
        <property type="gene ID" value="BRADI_5g26217v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824562"/>
    <dbReference type="eggNOG" id="ENOG502QSXH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062329_1_1_1"/>
    <dbReference type="OMA" id="FTELHYL"/>
    <dbReference type="OrthoDB" id="2015495at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009606">
        <property type="entry name" value="DEAL/Modifying_wall_lignin1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052222">
        <property type="entry name" value="DESIGUAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31769">
        <property type="entry name" value="OS07G0462200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06749">
        <property type="entry name" value="DUF1218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095709" description="Fiber protein Fb34" evidence="8">
        <location>
            <begin position="22"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ85302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="19870" checksum="BC340EB5DFA449DD" modified="2012-06-13" version="1">MASIVVIGLVLALDLLAFVLAIGAERRRSTAYVTVDGVGKPYCVYGTDASTWYGVGALSLLLAGQCVAMVASRCFCCGRALSPGRWRAFSGLCFVLCWLTFLIAELCLLAGSVRNAYHTKLSGYFISGPPRCAMLRKGVFAAGAAFAFLDLLFVELHYVFYAKARAAAASAPPIIHGGGAAGGIGMTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1J3W1</accession>
    <name>I1J3W1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100822924</name>
        <name evidence="11" type="ORF">BRADI_5g27670v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Intracellular vesicle trafficking and protein transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580906.1">
        <property type="nucleotide sequence ID" value="XM_003580858.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J3W1"/>
    <dbReference type="STRING" id="15368.I1J3W1"/>
    <dbReference type="EnsemblPlants" id="KQJ85531">
        <property type="protein sequence ID" value="KQJ85531"/>
        <property type="gene ID" value="BRADI_5g27670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822924"/>
    <dbReference type="Gramene" id="KQJ85531">
        <property type="protein sequence ID" value="KQJ85531"/>
        <property type="gene ID" value="BRADI_5g27670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822924"/>
    <dbReference type="eggNOG" id="KOG0080">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_1_1"/>
    <dbReference type="OMA" id="RVHKMDV"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01863">
        <property type="entry name" value="Rab18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000456">
        <property type="entry name" value="Ras-related protein RABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ85531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="215" mass="23709" checksum="C9249C60AC61AE70" modified="2012-06-13" version="1">MDSSSSSSSTAQQPDFDYLFKLLLIGDSGVGKSSLLLRFTADSFEDLSPTIGVDFKVKMVNIAGKKLKLAIWDTAGQERFRTLTSSYYRGAQGIIMVYDVTRRETFTNLSDIWAKEIDLYSTNQDCIKMLVGNKVDKESERAVTKKEGIEFAREYGCLFLECSAKTKVNVEQCFEELVLKILDTPSLLADASSGAKKNIFKQKAPEADAAAGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F8S2</accession>
    <name>A0A0Q3F8S2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100835029</name>
        <name evidence="2" type="ORF">BRADI_3g11930v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316296.1">
        <property type="nucleotide sequence ID" value="XM_024460528.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316297.1">
        <property type="nucleotide sequence ID" value="XM_024460529.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316298.1">
        <property type="nucleotide sequence ID" value="XM_024460530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F8S2"/>
    <dbReference type="EnsemblPlants" id="KQJ94642">
        <property type="protein sequence ID" value="KQJ94642"/>
        <property type="gene ID" value="BRADI_3g11930v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ94643">
        <property type="protein sequence ID" value="KQJ94643"/>
        <property type="gene ID" value="BRADI_3g11930v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ94644">
        <property type="protein sequence ID" value="KQJ94644"/>
        <property type="gene ID" value="BRADI_3g11930v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66454">
        <property type="protein sequence ID" value="PNT66454"/>
        <property type="gene ID" value="BRADI_3g11930v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66455">
        <property type="protein sequence ID" value="PNT66455"/>
        <property type="gene ID" value="BRADI_3g11930v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835029"/>
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        <property type="protein sequence ID" value="KQJ94642"/>
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    </dbReference>
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        <property type="protein sequence ID" value="KQJ94643"/>
        <property type="gene ID" value="BRADI_3g11930v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ94644"/>
        <property type="gene ID" value="BRADI_3g11930v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT66454"/>
        <property type="gene ID" value="BRADI_3g11930v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66455">
        <property type="protein sequence ID" value="PNT66455"/>
        <property type="gene ID" value="BRADI_3g11930v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1931893at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35755">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35755:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ94644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="52" mass="5714" checksum="141EF5EED8E8437F" modified="2016-01-20" version="1">MPQQAPQPGTASHVLDSVPLFVIVLGAVHVLALVYWIYKLASQKQPTRSKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FPW7</accession>
    <name>A0A0Q3FPW7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g47652v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLX family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FPW7"/>
    <dbReference type="STRING" id="15368.A0A0Q3FPW7"/>
    <dbReference type="EnsemblPlants" id="KQK00157">
        <property type="protein sequence ID" value="KQK00157"/>
        <property type="gene ID" value="BRADI_3g47652v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00157">
        <property type="protein sequence ID" value="KQK00157"/>
        <property type="gene ID" value="BRADI_3g47652v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FPW7"/>
    <dbReference type="OrthoDB" id="646377at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040353">
        <property type="entry name" value="FLX/FLX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405:SF28">
        <property type="entry name" value="DUF1421 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33405">
        <property type="entry name" value="PROTEIN FLX-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0287">Flowering</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK00157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="358" mass="39536" checksum="FE0E6D82AD293EFD" modified="2016-01-20" version="1">MESRASLHDVRGHQTLRQNLDNTEKEIQISQAERSDVDIVSGMQMKELMGMIQKIEAEMEERLQVLLKQDRFRADLSAGLQRLVVLVVNAAKRFEGDELDAELNRLQGHSEAIRRVFDYSRDYFTKQVEKMQTMVTSLIAMTKNVDKLHEEARNAEIRAQGLGTMVSGMAQAQVAQVGMCPYGSAYNNLPASYEQTTQAGQSREQTTQAGMRSYGSTYNNLPYAYPYAYFPPAEYQQATQDWAYAYAVPYACADPMASHQAVQASGYAGYPSSGSGQNAVPYYPGYLAGHGQNQNVSHYDVYVAPQYPMIVNNGPTTDAANIGGNAGFPAGQLQTSGRAGTNVAYERIPPSQGRHHRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FXA5</accession>
    <name>A0A0Q3FXA5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100832240</name>
        <name evidence="13" type="ORF">BRADI_2g11620v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
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    <sequence length="523" mass="57641" checksum="BCD1BA66AD58F1BC" modified="2016-01-20" version="1">MELPSAAMFQELFVLAILYAALHYLARAILPAKRAAGHASPPLPLPPGPRGYPVVGALPLLGRAPHRALATMAKLHGPIMHLTLGRQGVVVASTPAAARLFLKEHGASFLDRPTGDAAPTLLAYGAQDLVFAPYGPRWRRLRRECSIGLLGPQALVNWADTRREEVGHMVRAVLARRGEAVEVPEFLFCAMANMIGQAAVGRRVLDDAGGEATREFKEMVVELMTSAGLVNIGDFVPAVAWMDLQGLVRKMRRLWTRLDRVWARLLSEHEEVMAARQQEGARRLDLVDRLIACRREAGEEYGVTDLNIKALLNNVFTAGTDTSSSTIEWALAEMLLNPAIMRRAQAEMDALIGRDRLLRDSDTPNLPYLHAICKETFRKHPSTPLNLPRVSTEACNVQGYHIPKGTRLIVNIWGIGRDPAAWPDPTRFDPERFMTEQGKKVEPMGSHFELIPFGAGRRMCAGARMGVTLVHHMLGALVHAFDWEMPEGAAGVMDMEEEFGLALQKKVPVRAVARPRLAASAYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G809</accession>
    <name>A0A0Q3G809_BRADI</name>
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            <fullName evidence="5">CW-type domain-containing protein</fullName>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    </organism>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="CYSTEINE-TRYPTOPHAN DOMAIN-CONTAINING ZINC FINGER PROTEIN 5"/>
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        </source>
    </evidence>
    <sequence length="1481" mass="161067" checksum="B1054431EA07E42E" modified="2016-01-20" version="1">MMGCGRRREAAAGHEEGELDLEEGEALGGAARGTAHDELLDPDSLTYIDEKLHNLLGHFQKDFEGGVLAENLGSQYGGYGSFLPTYQRSPSGVSQSRSPAVPPNRGSASRSPYIPVESAEKSHFFKTALDSRRKDDCCQGTSSGTNGTGHQQKLNTTTKGPEQKAPKICIKVNTNRSLARNTAAIYSGLGLDISPSSSMDDSLDGSTGAPEPENLRNESPNTIFQIMTCHPIPGGVLLSPLAENILELRKRPKSVTNKHEAPALVDDKAEFNRDWCHVTSVARGNKDQMVNTYKYDEKKDHVPNVKSSKRRHNNSMIMNKMTMPELLDILDDTDSVLVPRSIKTEQASVEESTKLVADISNNHLKEIKNDPLKRRIRNKSSVGDIESIVDVGAVNLADDGNHPKGKANSKASTVRNAFKDLSKDSKTEPTLHEGFISKIKYDSDGYSDRPSTTSSQPQNVSRNNPSVGRDKGKVVHVKDELSLYRRKGMESSVNAESMDTIPESVGGNPSGMPKRKKKISSLQTAPSGKKLKVKAQKQLSNVTMKSYGEDMSVKPKETVSPGETDNGRLGDGNDDDHNISPLNFDRSTPVPSACVNEATKSSMSVPAAEPVVINERWACCDKCDKWRLLPYGMNPDILPKKWRCSMQSWLPAMSSCKISEDETTKALRALYMVPAPENDISLDGRHDISTSGICTAMAPLSSEGDTKFITTSGTPKVGSNVNVPSKLNMAEMSKPSKKLHSLISRNPDVDCFPKQKEKRKHVGSSDKGETVAEDLTRPESKSGIGVNHGNLRASKKIKKEFNEAAKNCPSEFEISKSSPSIKETPIFLHQDSGISPSLGKYGSSSSVKCNDDKVISDEGIRTSDAGQSDLPDSSSKVRKSKQRQLSKPGTETGSSDASVKHVVKEAISESNGAKEKPMPELKSLKTDDSKVAHARGPIVGRDSDRMYAEKECLSEQHQENIHLQHSLLSESATRKSMCYAQASTAATSSSSKVSSSHKSKVDFQETRASPVESVSSSPLRASGKNIVDQNKRYPRAVAENVPSQEPGRSVSSFSTGTQDLGSGSDHAKLHGSGCFNGDRHRHVLKGGELQKVKQDLLTNECSENEDSGLGGRNGQLNSVIARKVNSHVLSMHGNCDEKQPPAREHGRTLPHLNSNQCDHAKLTSGKLPAQVKPDKGHAEQKDLKTNPSAVNGSKQQTTLNNTANGDVSSKAKQSKNTVIENTKQVALNRDASNPINTSVLLKEARDLKHWSKRLKEKGDDLESASICFEACLKFLHVASLLEGPSIDRSKEGDSIQAMRLYSETGNLCGFCAREFERLKKMANAALAYKCVEVAYMKAAFYKHPGAIKDKHALQATSLMVPPAESPSSSASDVDNLNYQSTAAKAVSARALYSPQIAGNPIPRNNHHLMGLLAYADDINYAFDGTRKSQSSFAAYVADIGKVQVDGIALVREVLEFSFHNVKRLLQLIRQSLESINHECVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GPB5</accession>
    <name>A0A0Q3GPB5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycolipid transfer protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100836492</name>
        <name evidence="4" type="ORF">BRADI_1g06511v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GLTP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753013.1">
        <property type="nucleotide sequence ID" value="XM_014897527.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GPB5"/>
    <dbReference type="FunCoup" id="A0A0Q3GPB5">
        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GPB5"/>
    <dbReference type="EnsemblPlants" id="KQK12871">
        <property type="protein sequence ID" value="KQK12871"/>
        <property type="gene ID" value="BRADI_1g06511v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836492"/>
    <dbReference type="Gramene" id="KQK12871">
        <property type="protein sequence ID" value="KQK12871"/>
        <property type="gene ID" value="BRADI_1g06511v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836492"/>
    <dbReference type="OrthoDB" id="116883at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GPB5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902387">
        <property type="term" value="F:ceramide 1-phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902388">
        <property type="term" value="F:ceramide 1-phosphate transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035627">
        <property type="term" value="P:ceramide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120009">
        <property type="term" value="P:intermembrane lipid transfer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3520.10:FF:000005">
        <property type="entry name" value="Accelerated cell death 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3520.10">
        <property type="entry name" value="Glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036497">
        <property type="entry name" value="GLTP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014830">
        <property type="entry name" value="Glycolipid_transfer_prot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219:SF43">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08718">
        <property type="entry name" value="GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110004">
        <property type="entry name" value="Glycolipid transfer protein, GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glycolipid transfer protein" evidence="3">
        <location>
            <begin position="35"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK12871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="205" mass="22359" checksum="7412275EA9F95547" modified="2016-01-20" version="1">MSSEADKPLRRIGASFEELAAVAKQQPPAAMVAGDFSRACSHVSVLFGCLGIAFKFAEMDYVAKVDDLLDASKSISTLPSMVELDIQKGTVRQAGNHTRNLLRVKRGIDMVKVLFEQILVTEGNSLRDAASKAYAQVFAPHHGWAIRKAVGAGMYALPSKSQLLKKLNEDENSARAQMQSFVRSSGPVILYVEGLFTSRNLGIDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GPP5</accession>
    <name>A0A0Q3GPP5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC104581964</name>
        <name evidence="7" type="ORF">BRADI_1g07051v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cystatin family. Phytocystatin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229475.1">
        <property type="nucleotide sequence ID" value="XM_010231173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GPP5"/>
    <dbReference type="EnsemblPlants" id="KQK12960">
        <property type="protein sequence ID" value="KQK12960"/>
        <property type="gene ID" value="BRADI_1g07051v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581964"/>
    <dbReference type="Gramene" id="KQK12960">
        <property type="protein sequence ID" value="KQK12960"/>
        <property type="gene ID" value="BRADI_1g07051v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104581964"/>
    <dbReference type="OrthoDB" id="752087at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027214">
        <property type="entry name" value="Cystatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47116">
        <property type="entry name" value="PHLOEM FILAMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129168" description="Cystatin domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="6">
        <location>
            <begin position="37"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK12960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="116" mass="12932" checksum="A7B415412FF3CB16" modified="2016-01-20" version="1">MRTSSCLLLLAVVTIIAVVYPVTTSAQQPWHPIDGEDIDRPFYQILGGWAVMEHVKQAHDGLKFSKVFSGEKQDLSTSVKYHFVIIASDRGGKTDRYDAELIEGNPRRLISFAPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GSU6</accession>
    <name>A0A0Q3GSU6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100845761</name>
        <name evidence="10" type="ORF">BRADI_1g13430v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Nucleoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMP family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK13927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003561634.4"/>
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    <dbReference type="AlphaFoldDB" id="A0A0Q3GSU6"/>
    <dbReference type="STRING" id="15368.A0A0Q3GSU6"/>
    <dbReference type="EnsemblPlants" id="KQK13927">
        <property type="protein sequence ID" value="KQK13927"/>
        <property type="gene ID" value="BRADI_1g13430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845761"/>
    <dbReference type="Gramene" id="KQK13927">
        <property type="protein sequence ID" value="KQK13927"/>
        <property type="gene ID" value="BRADI_1g13430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845761"/>
    <dbReference type="OrthoDB" id="772609at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
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        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
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    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019358">
        <property type="entry name" value="NEMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31587:SF3">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31587">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN (DUF2215)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10225">
        <property type="entry name" value="NEMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="293"/>
            <end position="313"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="358"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="498"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00004575"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005748"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK13927.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK13927"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="543" mass="60086" checksum="E09EF49AFC319710" modified="2016-01-20" version="1">MARAAAGTSSASATTTWSSSEEAARPTSTAYSQSLSRRGFPPLLLVASLATLLILSSGDDAATYEASTAARPLKDVNLENPEVTFAPTSLGGQFCERVRISGVPRLRLQSYANQVRVTMNVSLSMREKFHRKIEICFHGNVSMGLCQCEIGEWQVLQDGMWNAVKSPYGIKYVDVKLADKKSTAFNLSIQEDFQKWRLACLGIGFVLLFLSPIVSKWAPFYYSSSMALGILLVVLIVLFQGMKLLPMGRKSLLYLTIYGSALGVGSYAVHYFSTLVASILENFGWSEEMHNPVSIFLLVAVVLTGAGFGYWMVRRFILSKDGSVDAGIAQFVKWAMRVVAIFFVMQSTLDPILALVALTTSWSVCSVLTANKVQEPMAPKQKQLKVSSQPKFTQVSPSTRQVQFLSPSSRMNIGRGPSNSPATQYGWNNLANGGLVPSTVTKRVLPNRDEDHYSTFHNIEPRKYSKREWEEFSEESTRNALMEHTATPEFAQWAADNAHRMRVERDDASEDDTIESSSNSSEETEEVDKATSLLSRLFAVTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GXM2</accession>
    <name>A0A0Q3GXM2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100839461</name>
        <name evidence="12" type="ORF">BRADI_1g24720v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010238680.3"/>
    </dbReference>
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        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GXM2"/>
    <dbReference type="EnsemblPlants" id="KQK15759">
        <property type="protein sequence ID" value="KQK15759"/>
        <property type="gene ID" value="BRADI_1g24720v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839461"/>
    <dbReference type="Gramene" id="KQK15759">
        <property type="protein sequence ID" value="KQK15759"/>
        <property type="gene ID" value="BRADI_1g24720v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839461"/>
    <dbReference type="OrthoDB" id="757982at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000006">
        <property type="entry name" value="B3 domain-containing transcription repressor VAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
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    <dbReference type="InterPro" id="IPR057743">
        <property type="entry name" value="Zfn_VAL1-3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011124">
        <property type="entry name" value="Znf_CW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46245">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS07G0563300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46245:SF10">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR VAL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07496">
        <property type="entry name" value="zf-CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25813">
        <property type="entry name" value="zf_VAL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51050">
        <property type="entry name" value="ZF_CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TF-B3" evidence="10">
        <location>
            <begin position="504"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="CW-type" evidence="11">
        <location>
            <begin position="751"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="660"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="899"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="56"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="905"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="922"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="957"/>
            <end position="967"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51050"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK15759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="995" mass="109987" checksum="3EEFCF7293C0A7EF" modified="2016-01-20" version="1">MSSPAPPRPPPPSAPTPATAPTATPMSVQPPPPQPKPPPPQQPSGSSAPPPPQFHPPASHQQPQPHPAASHQQPQPHTPVSHQQPQQPHPAGSHQQQQQQPHPPSSHQQRPRICFNTHCKDPKSEGPRRRGWRLRSGDFAELCDRCYGSFEQGSFCETFHSEVAGWRNCEACGKRLHCGCIVSIHAYALLDAGGVDCILCTRKSYAAAAMAPNQMCPTPTMHMPQNVADKKDSFVKSWRPPGGPYPSQWRQNSLWSVSGIQSDLQQRLAYEFDRPSGSEKLPPGRTFIHAQEKKFDDMHDRPTTPAGMNQIIRDRHADGHGQQTSMDPAHSYALYQREGPNPNSLHDHSHHGGESDSTSARKGIISEGCSSNIVSSSFKLDSRHPLESLLKENISLLPVGVGCTITNCPPVNGRNDIVRIIPHQPTSQTPSSAASSAQKQFYSHTVIDPEYQSHFRNGKPRMDAKARSQLLPRYWPRITDKELQHLSAEYPKRTNSKSVITPLFEKMLSASDAGRIGRLVLPKKCAEAYFPPISQPEGLPLKVQDGSGKEWVFQFRFWPNNNSRMYVLEGVTPCIQSMHLQAGDIVTFSRIDPEGKLIMGFRKSTTQEQILRQEQPTKPANAAVTAPPEVNVNVARPHEVNTENKNISPVDQAAVGKVENGGVAQKEGPGTARSSPGSLKRKATSVGPKIKRFRMDNEESMELKITWEEAQELLRPPLKAPSVVIVDGHEFEEYEEPPILGRRTYFVTDQSGENHQWAQCEDCSKWRKLPVGALLPSKWTCSDNKWDPERTSCESAQEATTEELAELFPIKAGAAKKPKARIEPDSIDVSDGLDTLANLAILGEGESLPSQPTTKHPRHRPGCSCIVCIQPPSGKGPKHKQTCTCNVCMTVRRRFKTLMLRREKRLSEKDTEEPRRKEVPQTGSDPPLGSTSPTSSPQKADANPDDAEDMVVDHRMSSSPVKNQIDLNIQPEREDEQSPKSNAVGAARLPRDNPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H3A5</accession>
    <name>A0A0Q3H3A5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_1g32219v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17053.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H3A5"/>
    <dbReference type="STRING" id="15368.A0A0Q3H3A5"/>
    <dbReference type="EnsemblPlants" id="KQK17053">
        <property type="protein sequence ID" value="KQK17053"/>
        <property type="gene ID" value="BRADI_1g32219v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17053">
        <property type="protein sequence ID" value="KQK17053"/>
        <property type="gene ID" value="BRADI_1g32219v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H3A5"/>
    <dbReference type="OrthoDB" id="672793at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H3A5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047627">
        <property type="term" value="F:adenylylsulfatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009150">
        <property type="term" value="P:purine ribonucleotide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01276">
        <property type="entry name" value="PKCI_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000005">
        <property type="entry name" value="Histidine triad nucleotide-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="5">
        <location>
            <begin position="171"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="45"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="265"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK17053.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="281" mass="30777" checksum="20F551CA2B0C8D80" modified="2018-03-28" version="1">MWPQSGCTRRRSSAANSQRQTAQSNGLPFLFFFLARWLLRNSRDEQHRMRDPGPAPDAAAPSSRPGENSIPQRPQPPLPAFSPMASAATLAAATSSLLRRSSPLLLRRPHGLRISRDVAPRRIGGSKKQETRTRNLELPVPRSVRHIASSTNEESAARAAAETADTGGPTIFDKIIAKQIPSNIVYEDEKVLAFRDINPQAPVHVLVIPKLRDGLTGLDKAEPRHAEILGQLLYAAKVVAEKEGVADGFRVVINNGEEGCQSVYHLHVHVLGGRQMKWPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H3N4</accession>
    <name>A0A0Q3H3N4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_5g10959v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable ATPase of unknown function. Its presence in a non-photosynthetic plant (Epifagus virginiana) and experiments in tobacco indicate that it has an essential function which is probably not related to photosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ycf2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H3N4"/>
    <dbReference type="STRING" id="15368.A0A0Q3H3N4"/>
    <dbReference type="EnsemblPlants" id="KQJ82787">
        <property type="protein sequence ID" value="KQJ82787"/>
        <property type="gene ID" value="BRADI_5g10959v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82787">
        <property type="protein sequence ID" value="KQJ82787"/>
        <property type="gene ID" value="BRADI_5g10959v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H3N4"/>
    <dbReference type="OrthoDB" id="652494at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33078">
        <property type="entry name" value="PROTEIN YCF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33078:SF102">
        <property type="entry name" value="YCF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ82787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="178" mass="20470" checksum="DCDFD777DC144046" modified="2016-01-20" version="1">MNYEFNRSSLAERRILLAHYQTITYSQTSCGANRFHLPSHGKPFSFRLALSGILVTGSIRTGRSYLVKYLTKNYDFPFIKVRGLLIPQERKHLFILSYTRGFYLENTMFHTKRFGCITTSSSALDLVALSNEALSISIPHKKSIIEKNTIRLTLHRQTCGLRAKVRSAQDHGTFFYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H8S3</accession>
    <name>A0A0Q3H8S3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g47586v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H8S3"/>
    <dbReference type="EnsemblPlants" id="KQK19319">
        <property type="protein sequence ID" value="KQK19319"/>
        <property type="gene ID" value="BRADI_1g47586v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19319">
        <property type="protein sequence ID" value="KQK19319"/>
        <property type="gene ID" value="BRADI_1g47586v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H8S3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK19319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="107" mass="11213" checksum="B233A5E567133FCF" modified="2016-01-20" version="1">MLLASAPSSVGWQRGAGTGREGGRGDDAVSAEDDVVAAVAGAEHAEVQRGAGYSPVAVGVAESESRPADRPICLLPPRSRRVRSILYMLCSALCDNRCRQDTVDHCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HB33</accession>
    <name>A0A0Q3HB33_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BURP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100834271</name>
        <name evidence="3" type="ORF">BRADI_1g50390v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228072.1">
        <property type="nucleotide sequence ID" value="XM_010229770.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HB33"/>
    <dbReference type="FunCoup" id="A0A0Q3HB33">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3HB33"/>
    <dbReference type="EnsemblPlants" id="KQK19773">
        <property type="protein sequence ID" value="KQK19773"/>
        <property type="gene ID" value="BRADI_1g50390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834271"/>
    <dbReference type="Gramene" id="KQK19773">
        <property type="protein sequence ID" value="KQK19773"/>
        <property type="gene ID" value="BRADI_1g50390v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="654134at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HB33">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044816">
        <property type="entry name" value="BURP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004873">
        <property type="entry name" value="BURP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31236">
        <property type="entry name" value="BURP DOMAIN PROTEIN USPL1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31236:SF7">
        <property type="entry name" value="BURP DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03181">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01045">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51277">
        <property type="entry name" value="BURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129235" description="BURP domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="BURP" evidence="2">
        <location>
            <begin position="358"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK19773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="587" mass="64993" checksum="642A8D861395B021" modified="2016-01-20" version="1">MRSNMLSLLSGFLLVMTVQQGLHTISVCADETSTVLDSTTVYWQKMLPNSPMPPAIVELLASSSSGRKIQNQKSQLSLWGLVRKLISFYGNNEVEKLIPFYGENNVEQIIPFYPGTKDEKNGLARIIPFYPGTKDEKNGLPKIIPFYPGTKDEKNGMPRIIPFYPGTKDDKNGLPKIIPFYPGTKDDKNGLPKIIPFYPETKDDKNGLQKIIPFYPGTKNEKNGLPKIIPFYPETKDEKNGLPKIIPFYPETKNEKNGMPKIIPFYPRSKDETNGLPRIIPFYPAGTQKDEINGLPKISMKRRTSSVQAEEDEHVTATEATEIPNHLNHFFFENEVPPHQNDAHNHIHHNHFTISNLFFLEESLTPGTTINLYILPSTSTGAALLPRAVADSIAMTTTSYPTILDTFSPVTRSMAEDIWTVLDVCEQSAMGGEDMKNQSCATSVESMAEFAASVLTGGDTHGLRAFSSPDVPAKGAMDGPRYKVTAARRATESMETMTCHDLSFPFPVFLCHSVNPTKVFEVTLEREEADQLAGAGDDGAERMEVLAICHLDTSAFEPEKMPGGIKPGEATVCHFIGKDTILWATAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HG27</accession>
    <name>A0A0Q3HG27_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Neprosin PEP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g43811v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HG27"/>
    <dbReference type="FunCoup" id="A0A0Q3HG27">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3HG27"/>
    <dbReference type="EnsemblPlants" id="KQJ92458">
        <property type="protein sequence ID" value="KQJ92458"/>
        <property type="gene ID" value="BRADI_4g43811v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92458">
        <property type="protein sequence ID" value="KQJ92458"/>
        <property type="gene ID" value="BRADI_4g43811v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HG27"/>
    <dbReference type="OrthoDB" id="784883at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004314">
        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF135">
        <property type="entry name" value="OS05G0341100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03080">
        <property type="entry name" value="Neprosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52045">
        <property type="entry name" value="NEPROSIN_PEP_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neprosin PEP catalytic" evidence="1">
        <location>
            <begin position="51"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ92458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="307" mass="34223" checksum="7C2815EF1B6D1B22" modified="2016-01-20" version="1">MKPSFYPYELQNRSSSVATKSLAQVPTVSCPRGTVPILQDRKGDITNFEGFHTMDGPSGELAVIKTVDDIYGSRVSINVYEPKVKEKTEDLSASWVLMLNKENASRMESVGVGSVVWPAFSGDNFARLHINWRDNTRDALCYDHRCPGFVHVNSRIGLGSKIQPVSVYNGPQHFIDVLLFKDPNTKNWWFLLGGAPVGYWPSSIFTHLKDKVTEAAWGGQVYGPTVQSNFPEMGSGHFAWEGFGKAAYVSNIKIVRENNKYYTPDTDSTFVRSTRPCCYAIDNYGQDEGGMHLYYGGPGDCSNNCNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I4X2</accession>
    <name>A0A0Q3I4X2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Uncharacterized protein ycf72</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_3g18416v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ycf72 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I4X2"/>
    <dbReference type="STRING" id="15368.A0A0Q3I4X2"/>
    <dbReference type="EnsemblPlants" id="KQJ95659">
        <property type="protein sequence ID" value="KQJ95659"/>
        <property type="gene ID" value="BRADI_3g18416v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95659">
        <property type="protein sequence ID" value="KQJ95659"/>
        <property type="gene ID" value="BRADI_3g18416v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3I4X2"/>
    <dbReference type="OrthoDB" id="597657at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038860">
        <property type="entry name" value="YCF72"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37377">
        <property type="entry name" value="RIBULOSE BISPHOSPHATE CARBOXYLASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37377:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009599"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ95659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="182" mass="20134" checksum="C190D1E7F55D2255" modified="2016-01-20" version="1">MGAFPSPPPWGWSTGFITTPLTMRRLPSQHLDPALPKLFWFTPTLPTCPTVAKQFWDTKRTSPDGNLKVADLPSFAISFATAPAALANWPPLPRVISMLCMAVPKGISVEGRFRFCACFKQFCLLHITISLESIIFYEELLNSITCCRRAFPIDIGTFVPETVVSPIIAPLGCKIYLFSPSP</sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
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                <person name="Barry K."/>
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                <person name="Vogel J.P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="11">Bd21</strain>
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    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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            <strain evidence="12">cv. Bd21</strain>
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        <property type="entry name" value="OS04G0168100 PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="OS07G0101800 PROTEIN"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ81432"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
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</entry>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tice H."/>
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                <person name="Bruce D."/>
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                <person name="Shu S."/>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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            <strain evidence="9 10">Bd21</strain>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
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                <person name="Shu S."/>
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                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
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        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="gene ID" value="BRADI_4g36250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828137"/>
    <dbReference type="Gramene" id="KQJ91208">
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        <property type="gene ID" value="BRADI_4g36250v3"/>
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    <dbReference type="KEGG" id="bdi:100828137"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="term" value="P:lipid droplet formation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="CDD" id="cd23995">
        <property type="entry name" value="Seipin_BSCL2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Seipin"/>
    </dbReference>
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        <property type="entry name" value="BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="Seipin"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="45"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ91208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="51257" checksum="244B3C03D8927571" modified="2016-01-20" version="1">MPLADHASSGRLSGSPPMDADEPAAASSSSSPTSDDAFFEAQDSIPPPPPPPPPPPSHPPPPPPPPPAPPLPSPPHTSSTLRRRPRRAKSLKEPDHVVTPSASAASTVTGVDEPVKAEPESSDVTSARRPPSPAEEEPNEKDAEAEAEVVARDYRPAAPPSILESLAVVIIKAVVFQVNALVSCLTFPVRLLQWWFLFVTDPVGSVRQAREWVLGAAGQATGAVTTWLGGGDGVGRVVARLVWGSLWAVYVCVVLCSLLVMAFLGGGLLVGKMVEEPVQMTENLNFDYTKPSPMAFVPVWRFVPPNQRMELEVSLTLPESDYNRRLGVFQVRAELLSADGKVVSASSQPCMLKFKSVHMHFIETFFQSLSLLSGYSSESQVITLKMRGIKEAFEPITGVRIILKQRAEFSPGAGIPEIYAASLKLEAELPLLKRILWNWRWTLFVWSSMGFFVFGLLLTLICCRPCIFPRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IZQ8</accession>
    <name>A0A0Q3IZQ8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polyglutamine-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Polyglutamine tract-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100840198</name>
        <name evidence="17" type="ORF">BRADI_2g58667v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="14">Interacts with POU3F2/Brn-2, ATXN1, TXNL4A, HTT and AR. Interaction with ATXN1 correlates positively with the length of the polyglutamine tract. Interacts with RNA polymerase II large subunit in a phosphorylation-dependent manner. Forms a ternary complex with ATXN1 mutant and phosphorylated RNA polymerase II. Interacts (via C-terminus) with TXNL4A and CD2BP2. Interacts (via WW domain) with ATN1 and SF3B1, and may interact with additional splice factors. Interacts (via WW domain) with WBP11; Leading to reduce interaction between PQBP1 and TXNL4A. Interacts with CAPRIN1. Interacts with DDX1. Interacts with SFPQ. Interacts with KHSRP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564919.1">
        <property type="nucleotide sequence ID" value="XM_003564871.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IZQ8"/>
    <dbReference type="STRING" id="15368.A0A0Q3IZQ8"/>
    <dbReference type="EnsemblPlants" id="KQK11187">
        <property type="protein sequence ID" value="KQK11187"/>
        <property type="gene ID" value="BRADI_2g58667v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840198"/>
    <dbReference type="Gramene" id="KQK11187">
        <property type="protein sequence ID" value="KQK11187"/>
        <property type="gene ID" value="BRADI_2g58667v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840198"/>
    <dbReference type="OrthoDB" id="42462at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IZQ8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21737">
        <property type="entry name" value="POLYGLUTAMINE BINDING PROTEIN 1/MARVEL MEMBRANE-ASSOCIATING DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21737:SF3">
        <property type="entry name" value="POLYGLUTAMINE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WW" evidence="16">
        <location>
            <begin position="288"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="16">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="438"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK11187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="565" mass="60978" checksum="8C2C4C0FDA4AC181" modified="2016-01-20" version="1">MNNSHGQPLPPGVGTWQPPPSNHPSQFQPSPRPHAYPTPYDTRPNGGNNQNTANVFYATQPFHPSPHPHAYPAPYDTRPNNGSNQDTSNLFYATQPNTPLLPAQSTPNAPTDPGNWQAHQTAHEVANHNGSAANIESAVQEAVLHEQDIETQQVIQNQRQANATSEPAEYGEDMLLSRRDPNALKEHLLKMTVDHRAEMANKRGKSLQHDNGNVEIGNGYGVPGGGAYHAGSFSSAQMNKPKDGADDLPEFLKQRLKARGILKDKAANDNSAAKQKADSQEGHNISAQELPPGWVEAKDPTTGASYFYNQSTGVTQWDRPGGAVNPVQHQAAPSLPENWEEALDKSTGQKYYYNTKTHATQWEPPTSVQPGVVPHASTDVAVQPTAQNADLCNPHMQRCSGCGGWGIGLVQPWGYCNHCTRVQNLPFQQYPSYSNNIVQSSGTNAPKIQGNVATKDRSSTKPPSGKANKKDNRKRNHAEDDELDPMDPSSYSDAPRGGWVVGLKGVQPRAADTTASGPLFQQRPYPSPGAVLRKNAEVASNGKKRGGMAAISKRGDGSDGLGEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J0T8</accession>
    <name>A0A0Q3J0T8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4220 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104585165</name>
        <name evidence="3" type="ORF">BRADI_4g40840v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010241176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J0T8"/>
    <dbReference type="EnsemblPlants" id="KQJ91960">
        <property type="protein sequence ID" value="KQJ91960"/>
        <property type="gene ID" value="BRADI_4g40840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104585165"/>
    <dbReference type="Gramene" id="KQJ91960">
        <property type="protein sequence ID" value="KQJ91960"/>
        <property type="gene ID" value="BRADI_4g40840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104585165"/>
    <dbReference type="OrthoDB" id="677696at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025315">
        <property type="entry name" value="DUF4220"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007658">
        <property type="entry name" value="DUF594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31325">
        <property type="entry name" value="OS01G0798800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
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        </location>
    </feature>
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        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
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        <location>
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            <end position="164"/>
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            <end position="365"/>
        </location>
    </feature>
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        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4220" evidence="2">
        <location>
            <begin position="63"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ91960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="722" mass="81156" checksum="140C541308DBC005" modified="2016-01-20" version="1">MQQQHLSVGNLTREATALAASPVGRLARIELLVTISCALLTVLVLLGSGRRATQSSALRFAVWAALMLSYPAVSYTIGLMQSASFRNELVVTWGCFLLLLLGTADGIAAYSLNDSDQQARTILNQALQVIYVFLLLLSYLGSLPLHLKAFLSLLLLLSVAKLGMRVTCFLRDGRDRVLTVDNKLVSDYMAREHGYSGADYDAGSMKGYKYVVAGEAAELHDGERDYWAIDPETNPDLVTVDRVWECQGRLLRSGPDADQDDDDDAAMAALELKDLCLSFALFKLLRRRLGRYPLHESGLNKTRDLVKVGLLAGEDHERMYRVVEVELGFLFDFYYARYRSPKETLMPDTALFAAVVATSLCTLFATAPDYYRRSPAPPQGYPNNIIVTTMFDIWLTRVIILLFLVLESFQYLALVFSDWHKVKTLCRYVLNEAWHNSPLLERTLRLICRVKLTRYWNGSLGQHSLLHACLVRHDKPPRVLRFLTSGRRTATADRRKLPGSVKRAIYGFLRTGLTRIQNGEHTLEKNGMLDNFYWAIAQESAVQTMMIWHVATAICDSRMRLSCRADAATKENHEVATTLSAYCAYLMASAPELLPDHSYDTQLLFQGVRRKAKKLLRGCRLKEDMHGKLPDSECNDGYEGVLADGKKLSDEIVHKMPDTAMRWRVLAELWVEMLLSVAPSDNATGHIQKLATGGELITHLWALLTHAGILEKPISRYQRKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J335</accession>
    <name>A0A0Q3J335_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glycerate dehydrogenase</fullName>
            <ecNumber evidence="9">1.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BRADI_3g00330v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-glycerate + NAD(+) = 3-hydroxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17905"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16659"/>
            <dbReference type="ChEBI" id="CHEBI:17180"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Photosynthesis; photorespiration; 3-phospho-D-glycerate from glycine: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.29" evidence="9"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92736.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J335"/>
    <dbReference type="FunCoup" id="A0A0Q3J335">
        <property type="interactions" value="778"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3J335"/>
    <dbReference type="EnsemblPlants" id="KQJ92736">
        <property type="protein sequence ID" value="KQJ92736"/>
        <property type="gene ID" value="BRADI_3g00330v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92736">
        <property type="protein sequence ID" value="KQJ92736"/>
        <property type="gene ID" value="BRADI_3g00330v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3J335"/>
    <dbReference type="OrthoDB" id="9991913at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3J335">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008465">
        <property type="term" value="F:hydroxypyruvate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009854">
        <property type="term" value="P:oxidative photosynthetic carbon pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000207">
        <property type="entry name" value="Glycerate dehydrogenase HPR, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF257">
        <property type="entry name" value="GLYOXYLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0323">Glycolate pathway</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="11">
        <location>
            <begin position="29"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="12">
        <location>
            <begin position="130"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ92736.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="379" mass="41344" checksum="E4438AC6163C2726" modified="2018-03-28" version="2" fragment="single">MAKPISIEVWNPSGKYRVVSTKSMPGTRWIRLLTDNDCRLEICTEKKTILSVDDILALIGDHCHGVIGQLTEDWGEVLFSALKRAGGTAFSNMAVGYNNVDVDAANRNGIAIGNTPGVLTETTAELAASLSVAAARRIVEADQFMRAGLYDGWLPHLFVGNLLKGQTVGVIGAGRIGSAYARMMIEGFKMNLIYYDLYQSTRLEKFVTAYGQFLKANGEQGVTWKRAGSMEEVLREADVISLHPVLDKTTYHLINPERLAMMKKEAVLVNASRGPVIDEAALVEHLKANPMFRVGLDVFEDEPYMKPGLAEMKNAVVVPHIASASKWTREGMATLAALNVLGKIKGYPVWGNPNAVEPFLDENATPPPACPSIVNAKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JLL5</accession>
    <name>A0A0Q3JLL5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100826774</name>
        <name evidence="14" type="ORF">BRADI_3g41987v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">May be a general defense protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235369.1">
        <property type="nucleotide sequence ID" value="XM_010237067.3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99248">
        <property type="protein sequence ID" value="KQJ99248"/>
        <property type="gene ID" value="BRADI_3g41987v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826774"/>
    <dbReference type="Gramene" id="KQJ99248">
        <property type="protein sequence ID" value="KQJ99248"/>
        <property type="gene ID" value="BRADI_3g41987v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826774"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JLL5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000157">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000532">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="500"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="535"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="692"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1110"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1140"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1197"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1220"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1250"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1281"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1331"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="97"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="767"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ99248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1362" mass="153921" checksum="4527299A8EE82A70" modified="2016-01-20" version="1">MEKQQISLQEPSAEDQQPPVPVPVMTASGRLWAPPLTHEDNRGFLHMLREKKERLGVGAVKVEVQFKDLTVEADVRVGRRALPTLLNSALNAAQELAASSHMCSTRKRPIKIINGASGTIQPSRMTLLLGAPGSGKTTFLKALAGKLDSSLKLKGKVMYNGEEVNPWTPQYLHAYISQYDLHHAEMTVRETIDFSSKMLGTNNEFEMLGEAIGRKKGAINKVDQDLDSFIKATTFGEGGNLTTNYIIKILGLSECADTLVGDEMRRGISGGQKKRATIGEMLVGLARCFFMDDISTGLDSSTTYEIVKFVQQMAHLMDLTVVISLLQPPPETLELFDDIILLCEGQIVYHGPREKATDFFEIMGFKCPSRKNVADFLQEVTSKMDQKQYWIGDENKYQYRPIEKFAESFRSSYLPRLVEDNLCRSNNTEKSKQAKTSASRRISRWNIFKACFSREVLLLKRNSPVHIFKTVQITLLALVISTVFLRTNMKHGSVLDANKYMGALFMAVVIVNFNGMTEIAMTIKRLPTFYKQRELLALPGWALLCSVYLISLPMSLVETGLWTSLTYFVIGYAPSVIRFIQHFLVLFTMHQMSMGLYRFLAAIGRTQVMANMLGTAALIAIYIFGGFVISKDDLQPWLRWGYWTSPFTYAQNAVSLNEFLDERWATEFHYANANTVGEAILKIRGMLTEWHWYWICVCVLFGFSLAFNILSIFALEFMNSPHKHQVNINTTKMMTECKNKKAGTGKVSTAPAVLPFRPLSLVFDHINYFVDMPKEMMKHGVTEKKLQLLQDVSGAFRPGVLTALMGITGAGKTTLLDVLAGRKTGGYIEGTIKVAGYPKKQETFSRISGYCEQSDIHSPNLTVYESLQFSAWLRLPSNIKSRQRDMFIDEVMDLVELTGLKNAMVGLAGATGLSAEQRKRLTIAVELVASPSIIFMDEPTTGLDARAAAIVMRTVRKTVDTGRTVVCTIHQPSIEIFESFDELLLMKRGGQIIYSGSLGPLSSNMLKYFEAIPGVPRIKEGQNPAAWMLDISSQTTEYEIEVDYAEIYRSSSLYRENLLLIDEMGKPAPNTEDLHFPPRYWQNFRAQCMACLWKQRCAYWKNSEHNVVRFLNTFAVSIMFGIVFWKIGSTIKKEQDVFNILGVVYGSALFLGFMNCSILQPVVAMERVVLYREKAAGMYSTLAYAIAQVAIELPYMLVQVFVFAAIVYPMIGFQMTASKFFWFVLYMALSFMYYTLYGMMTVALTPSTEIAAGLSFLIFIFWNVFSGFIIGRELIPVWWRWVYWANPAAWTVYGLMFSQLGDQTELILVAGQPDQTVREFLEGYLGLEDRYFNLVTCLHFAIIALFAFLFFISLKHLKFQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JQ32</accession>
    <name>A0A0Q3JQ32_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_3g51050v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JQ32"/>
    <dbReference type="FunCoup" id="A0A0Q3JQ32">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3JQ32"/>
    <dbReference type="EnsemblPlants" id="KQK00673">
        <property type="protein sequence ID" value="KQK00673"/>
        <property type="gene ID" value="BRADI_3g51050v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00673">
        <property type="protein sequence ID" value="KQK00673"/>
        <property type="gene ID" value="BRADI_3g51050v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JQ32"/>
    <dbReference type="OrthoDB" id="195679at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46502">
        <property type="entry name" value="C2 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46502:SF14">
        <property type="entry name" value="CALCIUM-DEPENDENT LIPID-BINDING (CALB DOMAIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK00673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="216" mass="24617" checksum="6DDE6C5CC3738391" modified="2016-01-20" version="1">METPYGVLELLLVSAEGLKHAHHLGPSVVAILFSESLMTLVASHCAHPCSSACACAGPQRHYVTIQCGELIRTSKITHGKHRKIWWNEKFRFPLSPVECRGLLAEVTLKIMERDKFSEDSLVGETRVNVGDIIREGIEREFLQMKPVPYNIVLQDGTYKGELKLGLKFLSGSSYVPPRQQQFVVYKPFLNIMLPCIPWRRFFFFCTRSNNGHRKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KZK4</accession>
    <name>A0A0Q3KZK4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g29272v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KZK4"/>
    <dbReference type="EnsemblPlants" id="KQK16561">
        <property type="protein sequence ID" value="KQK16561"/>
        <property type="gene ID" value="BRADI_1g29272v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16561">
        <property type="protein sequence ID" value="KQK16561"/>
        <property type="gene ID" value="BRADI_1g29272v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3KZK4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK16561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="110" mass="12057" checksum="C8DA3348CDF8598F" modified="2016-01-20" version="1">MRSSEARARHNKSNSLRNPDSAAESTPVSWALRIEAENKLMGHVKLQLDVAHLALATTTKAIGCRTSRSLEGETHWYPLASTIAKLQSGSLAAKGRKNEVEPPFDMSRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3MHG8</accession>
    <name>A0A0Q3MHG8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100824182</name>
        <name evidence="15" type="ORF">BRADI_2g10420v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the translocation pore. Directly involved in the pore formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567047.3">
        <property type="nucleotide sequence ID" value="XM_003566999.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3MHG8"/>
    <dbReference type="STRING" id="15368.A0A0Q3MHG8"/>
    <dbReference type="EnsemblPlants" id="KQK03886">
        <property type="protein sequence ID" value="KQK03886"/>
        <property type="gene ID" value="BRADI_2g10420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824182"/>
    <dbReference type="Gramene" id="KQK03886">
        <property type="protein sequence ID" value="KQK03886"/>
        <property type="gene ID" value="BRADI_2g10420v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824182"/>
    <dbReference type="OrthoDB" id="19656at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3MHG8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07305">
        <property type="entry name" value="Porin3_Tom40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000010">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM40-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10" id="KW-0626">Porin</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK03886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="338" mass="37230" checksum="61452257E5DCB3C7" modified="2016-01-20" version="1">MGSSVSHAAAPPPPPPGYSAPAPAYTAPPPYAAVNDPLLMPQAEAKEEEEEKVDYLNLRCPVPYEEIQREAFMALKPELFDGFRFDLNKVFGEKFALNHSLSMGSLEIPSQGADVIKVPTSSYEFGASFVDPKVMLIGRISQDGRLNARVKYDLPSGFCLKINAQLTNEPHYSQGMGTLDYKGKDSRSQFQFGNNGFFGGNYIQSVTKNLSLGTEAFWLGEQRKSGVGFLARYDTKKMVATGQIASTGVISLSYVQKVSDKVSLATDFMYNHMSRDVTASVGYDYILRQSRLRGKLDTNGVVSSLLEEQMAPGLNFILSAEVDHWKKDYKFGFGLTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3N985</accession>
    <name>A0A0Q3N985_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100831591</name>
        <name evidence="9" type="ORF">BRADI_1g08977v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752972.1">
        <property type="nucleotide sequence ID" value="XM_014897486.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3N985"/>
    <dbReference type="FunCoup" id="A0A0Q3N985">
        <property type="interactions" value="1518"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13262">
        <property type="protein sequence ID" value="KQK13262"/>
        <property type="gene ID" value="BRADI_1g08977v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74159">
        <property type="protein sequence ID" value="PNT74159"/>
        <property type="gene ID" value="BRADI_1g08977v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831591"/>
    <dbReference type="Gramene" id="KQK13262">
        <property type="protein sequence ID" value="KQK13262"/>
        <property type="gene ID" value="BRADI_1g08977v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74159">
        <property type="protein sequence ID" value="PNT74159"/>
        <property type="gene ID" value="BRADI_1g08977v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831591"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3N985">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000988">
        <property type="entry name" value="Leucine-rich repeat protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000880">
        <property type="entry name" value="Leucine-rich repeat protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055164">
        <property type="entry name" value="EDR1/CTR1/ARMC3-like_pept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF1">
        <property type="entry name" value="INHIBITOR OF NUCLEAR FACTOR KAPPA-B KINASE EPSILON SUBUNIT HOMOLOG 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14381">
        <property type="entry name" value="EDR1_CTR1_ARMC3_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="772"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="460"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK13262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1101" mass="123013" checksum="3BF4E67F5B53FBF4" modified="2016-01-20" version="1">MAPAVLDPDAGDLPARGSAAAADDIAGNTWDLAPFSPPPAALRGGEIFIYRNTYNLVPRSIGECRRGGLRALKFFGNDVEVLPPEAGELDALESLQVKVSAPRVSGAVLRRMRALRELELSMVPPRPSACSILAEIAGLKCLTKLAICHFSIRFLPPEIGSLRKLQELDLSFNKLKNLPNCITELGALKFLKVTNNKLVDLPSGISSLRCLESLDLSNNRLTSLGSVKLVSMLTLQYLNLQFNRISHTLIVPSWICCDMRENGENAVKRDRLQYLGISSVSSSAEPGTASCACNGALSCSHTETSPNLKAHATQKMKKGWKRRDCLQQQARQERLDSSRSKLSENDVEEMAVNMTEDECLSSLHDIENKPVMKGIAEDTSVQDLLKETSSEDLSCIVDYDSDGLIKDTGMMLQDHHDNGSGIVTDPACLSRSSIHNIENELEDTASSTCKVVHVVEENPSETSKFTSKSKRHPDMDNNPKPSKCPRPIDECSKLSYKYSVESFCSIDDHLPDGFYDAGRDMPFMPLEEYERSLGLYAREVILLDREKDEELDAIASSAQLLLSSLKRPVFSETDEDAGQDLLRASVLALFVSDCFGGCDRSASLRITRRAIVSLRKEQPFICTCSAGNMCYSNESSKQINTPMGHFDFTGLCDKSIRIIKERRNSGIVPIGALQFGVCRHRAVLMKYLCDRADPPIPCELVRGHLDYTPHAWNVVPVRQGNIWVRMIVDACYPTNIKEETDPEYFCRYVPLSRLHLALDDESYTPRSSFPSVSLCKEIEATASSAVYHCKIGAVDAAAKIRYLDTRSASNDEVKNFEYKLLGEVRMLNALRKHRSIVDIYGHQLSSKWVQNDSDKEYRIMQSIILMEYVKGGSLKAFLAKLLKNGEKHVPIDLAFYIAREVACALLELHRKLVIHRDIKSENVLVDLDSKGHGAPVVKLSDFDRSIPLHSLSHTCCIAHLGTYPPNVCVGTPCWMAPEVVQAMHEKIQYGLEVDIWSFGCFILEMLTLHIPYQGLPDSQIYDLIKRKKQRPRLTRELEAFWTMDEPITRLNLGITCDAHAEKLRLLIDLFYKCTRGIASRRPKAEQIYNLLCSLPTCYDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NAQ2</accession>
    <name>A0A0Q3NAQ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g12705v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NAQ2"/>
    <dbReference type="EnsemblPlants" id="KQK13822">
        <property type="protein sequence ID" value="KQK13822"/>
        <property type="gene ID" value="BRADI_1g12705v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g12705v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NAQ2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036297604" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK13822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="10027" checksum="01F4A879C1F4A32F" modified="2016-01-20" version="1">MECNLAIHIIMVISYILATPCSLANTHHQQSNLGESSGCIRIYFRMIKHLMGGGHLEFWNKRLRLQVVDQMYRRVWLQVQGVLASSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NL68</accession>
    <name>A0A0Q3NL68_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g39222v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18166.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NL68"/>
    <dbReference type="EnsemblPlants" id="KQK18166">
        <property type="protein sequence ID" value="KQK18166"/>
        <property type="gene ID" value="BRADI_1g39222v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18166">
        <property type="protein sequence ID" value="KQK18166"/>
        <property type="gene ID" value="BRADI_1g39222v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NL68"/>
    <dbReference type="OrthoDB" id="673425at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48239">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48239:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK18166.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="57" mass="6184" checksum="29A092D1D6CA161B" modified="2018-03-28" version="2">MTGIGTTKGTKETRTTTTNSSANIFPKSRKLSDNGFVKSSRMLIGKRIGVGLIYFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGN9</accession>
    <name>A0A2K2CGN9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g11610v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CGN9"/>
    <dbReference type="EnsemblPlants" id="PNT61188">
        <property type="protein sequence ID" value="PNT61188"/>
        <property type="gene ID" value="BRADI_5g11610v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61188">
        <property type="protein sequence ID" value="PNT61188"/>
        <property type="gene ID" value="BRADI_5g11610v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CGN9"/>
    <dbReference type="OrthoDB" id="1912352at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CGN9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF204">
        <property type="entry name" value="OS11G0460800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT61188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="434" mass="47693" checksum="44FC2860FF10ADFF" modified="2018-03-28" version="1">MLDMPGGARAQCHDGSSCRRLAGGRSRGRSGCSGRARLMDTAACGHLPGREKKLLQQCLRRILPACAQTLKNAGAGSDSDTTPAVRGYMNTLSSSQIPFMLVVNHIAAGMTIPTLALEIDISNKESGEEPLLNLKGYFAGNPMTDDRFDTAGKIQFFHGMGVIPNELYEIAKENCRGNYSDPPSASCAESMQAIDILTKDINLSHILAPSCETIWSPRIQQAAARDGASLLTADDSSGDDIQFLFKCRSDSHQLSYIWANDEAVRESLAVRKETKGEWKRCDFDIPYTKDITSTVEHHLSLRKEGYPALIYSGDHDSKFSFVGTQAWIRSFNLSITDDWRPWYVDGQVAGALGILLQSTSPRTASPCSQDGFRVNLYERLVSIGSAPVSMDRTIECLLHLINIGNQRGSPQESTLRVCNLRLLPCFAFFKLRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHG6</accession>
    <name>A0A2K2CHG6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100844647</name>
        <name evidence="3" type="ORF">BRADI_5g15600v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHG6"/>
    <dbReference type="STRING" id="15368.A0A2K2CHG6"/>
    <dbReference type="EnsemblPlants" id="PNT61474">
        <property type="protein sequence ID" value="PNT61474"/>
        <property type="gene ID" value="BRADI_5g15600v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61474">
        <property type="protein sequence ID" value="PNT61474"/>
        <property type="gene ID" value="BRADI_5g15600v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="444127at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CHG6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF12">
        <property type="entry name" value="ACYL TRANSFERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT61474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="261" mass="27940" checksum="C869AB4A87878235" modified="2018-03-28" version="1" fragment="single">PRAVSLTQNNPSESNPQIACTKTSSTSRGNPESAPLPASAGEGRAAAAPNLPLPQTLFPKLILLVLEVMQFACGGFVVGFRFSHAVADGPGAAQFTTAAGEIAPGRAGPSVKAAWGREAIPTELRLQYLAMDISTDYIEHFKARFLEHAGPKCSAFEVLIAKAWQARTRAARFACGTPVHVCFAMNARSALPTSPRAIPDGFYGYCYYIMRVSAPAEAVSDAPLHDVVRLIHDGKKRLPSEFARWSRGEMNDGDGDPNQIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHZ3</accession>
    <name>A0A2K2CHZ3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g18206v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHZ3"/>
    <dbReference type="EnsemblPlants" id="PNT61644">
        <property type="protein sequence ID" value="PNT61644"/>
        <property type="gene ID" value="BRADI_5g18206v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61644">
        <property type="protein sequence ID" value="PNT61644"/>
        <property type="gene ID" value="BRADI_5g18206v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CHZ3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CHZ3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT61644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="311" mass="33289" checksum="9B9B3FA76D449D5C" modified="2018-03-28" version="1">MKSKRKIEMFDEKSKRACLPSVEDESATLAVGFDMPGDHSDDETDDGQYQDLSIWGESQEDGFDELYVCSEEEDTEEEKRVGGGRRVRHSEHRDPRGSHRDEPGGLGEGARGLIHGGMHDLSDDEPVEDGVRWVGHNTSTGGMHRRGHGSRGLSSELSIGREEEGYQHSEGRACGLDDGVCGLGGDLDTRAASSATATTTARVGSTTTKATACTAGRATPSLASASSACPWTQATRTELAISVVCVGAEAMVVACLETELAAVVTWAMASACAMSWPSSAHPWTQARQTRTSVWLQNLELLIAARKKLPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CKZ6</accession>
    <name>A0A2K2CKZ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g07252v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CKZ6"/>
    <dbReference type="EnsemblPlants" id="PNT62699">
        <property type="protein sequence ID" value="PNT62699"/>
        <property type="gene ID" value="BRADI_4g07252v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62699">
        <property type="protein sequence ID" value="PNT62699"/>
        <property type="gene ID" value="BRADI_4g07252v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CKZ6"/>
    <dbReference type="OrthoDB" id="671539at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20151" checksum="9409AF266980D8BC" modified="2018-03-28" version="1">MEGSPSIFLVVVTDAFNSLFLAGRTDAQQGHCVVAWAKVCLLIRLGGLGVRDANLLRWPWLQRSEVEKPWASFRIRFSSRILALFREACYSVVGNGRFTLFWSDNWIDGKSVESLAPVVFAAVGRRITRTCSVADALARSAWIRDISGPLNAQGMVEFLQLVGTLEAVVLIPGQEDVLRWR</sequence>
</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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            <strain evidence="1">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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            <dbReference type="EMBL" id="PNT63077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="30" mass="3655" checksum="4BD29A7E7C31A7E0" modified="2018-03-28" version="1">MRIDQLAKIYRMINYILPDLLSWHGKLKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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            <strain evidence="2">Bd21</strain>
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    <reference evidence="3" key="3">
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
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    </dbReference>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="1">Bd21</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
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    </evidence>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="EMBL" id="PNT68631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
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</entry>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </dbReference>
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    </dbReference>
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        <location>
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        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT69254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="302" mass="32237" checksum="61FC1F1C25854F34" modified="2018-03-28" version="1">MRCKRHPFQAGGGVCATCLRERLFVLAAAQNDASSPTTPAPPPPAASAAAAGPPAFPRSVSPYVSRRKSDSSGGALRNHPSLLFFRTPQVGPASAYGAGGALEEGDIGYEKRRAGKFSVLATLFGNHHPHQQHHNRSEVDKDKGGSGNKERKSRSWLAGIIPRRRKKQAPVADATALPPSSSPPPRRSCRAVSNRGLSPERGSHGSGEESSSPADPPWRPFPSPMRKTPCRRRQTNSMPSGLAVCLSPLVRPSPGRRHRAAQPPDPGTFSCELRPSPLHSVSSAANVARCRSRKLADAGRFR</sequence>
</entry>
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        </submittedName>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblPlants" id="PNT70981">
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        <property type="gene ID" value="BRADI_2g20893v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70981">
        <property type="protein sequence ID" value="PNT70981"/>
        <property type="gene ID" value="BRADI_2g20893v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D9L8"/>
    <dbReference type="Proteomes" id="UP000008810">
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT70981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="100" mass="11362" checksum="C5190E7FD4055A3E" modified="2018-03-28" version="1">MSNEVTENLATKLSLVGHHPISWRRRLFGAKMSKVLIRLLRNPSVRQRCSIVTPATGDGSGLLDLTNGIEDMERKTDELASSVKWTLWMTCIEDSVRYSW</sequence>
</entry>
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    <accession>A0A2K2D9S5</accession>
    <name>A0A2K2D9S5_BRADI</name>
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            <fullName evidence="2 3">Uncharacterized protein</fullName>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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            <begin position="69"/>
            <end position="90"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="100" mass="11413" checksum="0BCF000D987E7D68" modified="2018-03-28" version="1">MGGGLVLSLLSRPDNIYCEKGKTLPSFVFPTGLQGQAGGRVKVMEQSWTRSLLRRCHTKKSSSGRRRSPFFLTWLANHAMYILLFMLPFYSWKYLPIDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
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        <name evidence="2" type="ORF">BRADI_2g31735v3</name>
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    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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    <reference evidence="2 3" key="1">
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT71581.1"/>
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        <property type="protein sequence ID" value="PNT71581"/>
        <property type="gene ID" value="BRADI_2g31735v3"/>
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        <property type="gene ID" value="BRADI_2g31735v3"/>
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    <dbReference type="InParanoid" id="A0A2K2DBD7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EMBL" id="PNT71581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71581"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="105" mass="11434" checksum="205111C2192AA32F" modified="2018-03-28" version="1">MNYSQLPPPTTDAGERAAAATCGTLLPTRPPATTFFPRRGRASSGGGRREGARSGRPRHLLPTRPLGYHLLPPARASERRWRPTPQFVSPPTLSPPPNRVCMRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DBK0</accession>
    <name>A0A2K2DBK0_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g33277v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT71654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DBK0"/>
    <dbReference type="EnsemblPlants" id="PNT71654">
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        <property type="gene ID" value="BRADI_2g33277v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT71655">
        <property type="protein sequence ID" value="PNT71655"/>
        <property type="gene ID" value="BRADI_2g33277v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71654">
        <property type="protein sequence ID" value="PNT71654"/>
        <property type="gene ID" value="BRADI_2g33277v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71655">
        <property type="protein sequence ID" value="PNT71655"/>
        <property type="gene ID" value="BRADI_2g33277v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DBK0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="41" mass="4277" checksum="BA2F4FDBD24F98EB" modified="2018-03-28" version="1">METWTRASLSLSSPSPRHAQFGLKDVTTDEPMMPGGGGGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">BRADI_2g33545v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DBL0"/>
    <dbReference type="EnsemblPlants" id="PNT71670">
        <property type="protein sequence ID" value="PNT71670"/>
        <property type="gene ID" value="BRADI_2g33545v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71670">
        <property type="protein sequence ID" value="PNT71670"/>
        <property type="gene ID" value="BRADI_2g33545v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DBL0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
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            <begin position="9"/>
            <end position="36"/>
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    </feature>
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        <location>
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            <end position="105"/>
        </location>
    </feature>
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            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT71670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="187" mass="20476" checksum="4EBF5B1DDE21FA02" modified="2018-03-28" version="1">MDPLLVGHYVCHRCKKWYQRWEALYGHQSNHHGKKEYYLPRLTLSSVSGGGGCWDEPPRYQPWHVKSAHELDETRCRRASSAPSAAAASAHQRPPSGTWRAPTAASRPPLPAALVVLRHACHLPNHNGKLGCAWLSKRRGQCTARDGGWGGAALGGRVGGMDGARAGMAREENCVAREESMENVHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DCT9</accession>
    <name>A0A2K2DCT9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100845682</name>
        <name evidence="3" type="ORF">BRADI_2g39127v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DCT9"/>
    <dbReference type="STRING" id="15368.A0A2K2DCT9"/>
    <dbReference type="EnsemblPlants" id="PNT72087">
        <property type="protein sequence ID" value="PNT72087"/>
        <property type="gene ID" value="BRADI_2g39127v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72088">
        <property type="protein sequence ID" value="PNT72088"/>
        <property type="gene ID" value="BRADI_2g39127v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72087">
        <property type="protein sequence ID" value="PNT72087"/>
        <property type="gene ID" value="BRADI_2g39127v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72088">
        <property type="protein sequence ID" value="PNT72088"/>
        <property type="gene ID" value="BRADI_2g39127v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="594396at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DCT9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046533">
        <property type="entry name" value="DUF6598"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065:SF190">
        <property type="entry name" value="DUF6598 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065">
        <property type="entry name" value="OS07G0486400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20241">
        <property type="entry name" value="DUF6598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6598" evidence="2">
        <location>
            <begin position="145"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT72087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="398" mass="44619" checksum="E4AB06D13D478339" modified="2018-03-28" version="1">MKNINQMDPLQTEIRNESTRAHGPLKRSPRRRTRSKSDLDGRRGSRKGIIMAGGDSSSSSPRAAVVANADDEPPPPWEKQKPPPQPAAEEEEDVDPRFNEYDPKTGEIINIRFFNSRLDLDEESSFGPMRHTDKVFEDGFRLKNTVNVVSVQIVSSDYGYPLNVYGSILARDSLDRKCVYVFQRGQDNCQFISSKDDLLTLTGPKRGFMVCDDIFFEINLKVKDAQGRKVDDERLSKGLIEVDAIRRLSFQPRYKVETETLVSMNSILDLNYTFLRRTVEGTVEIRILEGPADFHGRVTACTSSIPCNIVLHDRKVSGVLTAGDDGVVQTARRVVGVSVDEMLLLTVSAAAAAADDDDEFSARTTVEFTPRRNGYGIEKIVCGDHRMSLKVTWSIVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DE63</accession>
    <name>A0A2K2DE63_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4283 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g45635v3</name>
    </gene>
    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DE63"/>
    <dbReference type="FunCoup" id="A0A2K2DE63">
        <property type="interactions" value="493"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DE63"/>
    <dbReference type="EnsemblPlants" id="PNT72529">
        <property type="protein sequence ID" value="PNT72529"/>
        <property type="gene ID" value="BRADI_2g45635v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72529">
        <property type="protein sequence ID" value="PNT72529"/>
        <property type="gene ID" value="BRADI_2g45635v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DE63"/>
    <dbReference type="OrthoDB" id="8026949at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053253">
        <property type="entry name" value="Sex_diff_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087:SF31">
        <property type="entry name" value="OS06G0482850 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33087">
        <property type="entry name" value="OS07G0539200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="412"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT72529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="77982" checksum="DB7A57E2069ADFEC" modified="2018-03-28" version="1">MASTPGGAQPSTMERRWRESSPSSGSESERSYREVRQELPQEMAGKCFNCLGDDHAAALCPNPTRCLRCKEGRAMWLGCAGSVAAQVVPRRLGLGGGAQGPAPRGPVRPAVVAQEPPVARGMEVPTTQASPVLIHGGQRPGLALLARLGRVGASACENLTGEEAASALTAVAPAALPWGAASLRPRVETCIISRTAQLKEEEAALEWSLVASVTGTRPRVPLSSVSLALVGHFPGLEGTFTAHRFWPDDFLVVFRSREGRDVVLAAGEIGGRGFSLRFSAWNRFRQAVGRTLYFRVHLELDGVPSQAWSFGTAASILGPSCVVERLGSTTASREDMGRFRVFPWTPDPNLVPRETTLQIVEPPALVDGDDDLMLAPEDLIPEELQLLEYKVLIHLLQVDNQRESTDRTLSMDGPSDNGDSGNDGDPGCSYGGAHDAREAWRNYFSCVRGQVDSDDFGNRPRGTWVGGRVAEGCLMVADWSRLSATAPAYVPALLPWVEMVEGSLAVGMEEVVAMLSLLPGCEVVVDDGWDPMRLEACILPCPTLTAPVLAGPDSPIPVLAGMDTSVVGSADSTGGTTVLPGSARLGWRETPSEGGFAGHDAAGSEYIHGEHHAGGEVASGLPGCGFEGKCGAGHCGLQGDDAAFAFASVAAVGGATVEARIAGRLTPAVGVKREERELMHRLGIAREGEQIGEEALEAYTKLFVRPLSSEHIAAILALFGWEPDVLPLETGEGDAITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DFU1</accession>
    <name>A0A2K2DFU1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BRADI_2g53870v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="11"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DFU1"/>
    <dbReference type="STRING" id="15368.A0A2K2DFU1"/>
    <dbReference type="EnsemblPlants" id="PNT73129">
        <property type="protein sequence ID" value="PNT73129"/>
        <property type="gene ID" value="BRADI_2g53870v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73129">
        <property type="protein sequence ID" value="PNT73129"/>
        <property type="gene ID" value="BRADI_2g53870v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DFU1"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DFU1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000084">
        <property type="entry name" value="probable methyltransferase PMT23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF1101">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="62"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="PNT73129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="686" mass="76226" checksum="6C391EFCD0292C94" modified="2018-03-28" version="1">MAISSALSFLSDRKRPIAIAVILFILLSSLFLLLSPAPSALPFFYSPTARFSSSFSPIAASAPPQTPIPVSADASPPQTPVGAFGGSGSNSADPPPADTAAPHGDATAAAVHLHQPDHSPPPPAAAEVSASVDKKEIATVVSGERGGDEGGEGGGAGAEEVLSWDLCEVGKRVEPADYIPCLDNVKAVKALKSTRHMEHRERHCPTEPRPRCLVPLPAGYRLPLPWPRSRDMIWYNNVPHPKLVEYKKDQNWVRKSGNYFVFPGGGTQFKAGVTRYIRFIEQIMPQINWGTHTRTVLDVGCGVASFGGYLLDRNVITMSFAPKDEHEAQIQFALERGIPALLAAIGTQKLPFPDNAFDVIHCARCRVHWYADGGKPLLELNRVLRPGGYYIWSATPVYRRGKRDEEDWNAMVTLTKSICWRTVVKSKDVNKIGVVIYQKPVSNSCYIERKNNEPPLCTARDDHSPWYTPLDSCLLLPVVSSSGEGNGWPISWPERLNMRYPSRSDNSSTQFSQEKIDSDTKQWSGLVSEVYFSGFAIDWSSIRNVMDMNAGFGGFAASLIDRPLWVMNVVPFDQPDTLPIIFNRGLIGVYHDWCESFNTYPRTYDLLQMSYLLQSLTNRCDIIEVAAEIDRILRPGRWFVLHDTIGVIRKMDQVLRSLHYKTAIVKQQLLVARKSFWRPGSTDDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGN2</accession>
    <name>A0A2K2DGN2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g58562v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGN2"/>
    <dbReference type="EnsemblPlants" id="PNT73446">
        <property type="protein sequence ID" value="PNT73446"/>
        <property type="gene ID" value="BRADI_2g58562v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73446">
        <property type="protein sequence ID" value="PNT73446"/>
        <property type="gene ID" value="BRADI_2g58562v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DGN2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT73446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="75" mass="8618" checksum="AAED1C1FDE6B7F93" modified="2018-03-28" version="1">MSGFMIKAQSPQQQNPLKMLLHSQVTCRQAFLHGFVFVELMSSLTCTNKNCFTIISHPKRYFTLSAFTIYFTVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DJL8</accession>
    <name>A0A2K2DJL8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g15291v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DJL8"/>
    <dbReference type="EnsemblPlants" id="PNT74476">
        <property type="protein sequence ID" value="PNT74476"/>
        <property type="gene ID" value="BRADI_1g15291v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74476">
        <property type="protein sequence ID" value="PNT74476"/>
        <property type="gene ID" value="BRADI_1g15291v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DJL8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036319252" description="Secreted protein" evidence="1">
        <location>
            <begin position="43"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT74476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12790" checksum="739409BDB3229204" modified="2018-03-28" version="1">MVLLHVRRLSLLSMVLHCRSMFSRLSLLSMVMLSLSLSPIHGAAPVAAAATARPCVGGGSRRCACGGDSGQHRAMCFWRASFFLRHTIEEAKREWCARDSRVTEAFPEKREQSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKJ0</accession>
    <name>A0A2K2DKJ0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glycerophosphodiester phosphodiesterase</fullName>
            <ecNumber evidence="2">3.1.4.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100824856</name>
        <name evidence="10" type="ORF">BRADI_1g22187v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a sn-glycero-3-phosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:83408"/>
            <dbReference type="EC" id="3.1.4.46"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.46" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235901.2">
        <property type="nucleotide sequence ID" value="XM_010237599.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DKJ0"/>
    <dbReference type="STRING" id="15368.A0A2K2DKJ0"/>
    <dbReference type="EnsemblPlants" id="PNT74804">
        <property type="protein sequence ID" value="PNT74804"/>
        <property type="gene ID" value="BRADI_1g22187v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824856"/>
    <dbReference type="Gramene" id="PNT74804">
        <property type="protein sequence ID" value="PNT74804"/>
        <property type="gene ID" value="BRADI_1g22187v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DKJ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08602">
        <property type="entry name" value="GDPD_ScGlpQ1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000023">
        <property type="entry name" value="Glycerophosphodiester phosphodiesterase GDPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620:SF4">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044576649" description="glycerophosphodiester phosphodiesterase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="59"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT74804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="388" mass="43347" checksum="06E7218C22524C88" modified="2018-03-28" version="1">MVSLGMTVAAAALLLALVLGSTSNGAAAARPMAAPGPAPGPWAGLGMEAEKQLLQTSRPYNIAHRGSNGEFPEETAAAYLRAVDEGADFIEADVAATKDGHLVCFHDMTLDDTTDVAAHPEFAGRRRTLEVQWANVTGFFITDFTLAELKTLRTKQRWEFRDKSHDGVSPIITFDEFISIALNAKRVVGIYPEMKSPVFINQHVKWADGKKYEDKFIGTLKKHGYGGKYMSPAWKAKPLFIQSFAPTSLIYAAELTDSPKVFLIDDVTVRTEDTNQSYDEITSGQYLDYMKEYVVGIGPWKDTVVPPTKDNRLAAPTDLVAVAHARGLQVHPYTYRNENKFLRFNFRQDPYAEYDYWLNVIGVDGLFTDFPASLRRFQQWTAKNRSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DL14</accession>
    <name>A0A2K2DL14_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_1g25430v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PsbQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DL14"/>
    <dbReference type="FunCoup" id="A0A2K2DL14">
        <property type="interactions" value="1974"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DL14"/>
    <dbReference type="EnsemblPlants" id="PNT74969">
        <property type="protein sequence ID" value="PNT74969"/>
        <property type="gene ID" value="BRADI_1g25430v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74969">
        <property type="protein sequence ID" value="PNT74969"/>
        <property type="gene ID" value="BRADI_1g25430v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DL14"/>
    <dbReference type="OrthoDB" id="497707at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DL14">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009654">
        <property type="term" value="C:photosystem II oxygen evolving complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009767">
        <property type="term" value="P:photosynthetic electron transport chain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.290">
        <property type="entry name" value="Oxygen-evolving enhancer protein 3 (PsbQ), four-helix up-down bundle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023222">
        <property type="entry name" value="PsbQ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008797">
        <property type="entry name" value="PSII_PsbQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054099">
        <property type="entry name" value="PSII_PsbQ_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33399">
        <property type="entry name" value="OXYGEN-EVOLVING ENHANCER PROTEIN 3-1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33399:SF3">
        <property type="entry name" value="OXYGEN-EVOLVING ENHANCER PROTEIN 3-1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05757">
        <property type="entry name" value="PsbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101112">
        <property type="entry name" value="Oxygen-evolving enhancer protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="8" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT74969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="280" mass="29636" checksum="F93F4B9B1D9B05A2" modified="2018-03-28" version="1">MPHGAPALAPNLSSQIRLATAKAPSYISGTLAATGGARDRSLPPPRSPRENAKLEMAQAMASMTGLTSAQGVRLPGPAGRRGASKLTVRASAEAETAGRRAVLGLMASGVAGGAFAQAVLAAVNPIKVGPPPLPSGGLPGTENSDEARDFALPLKNRFYLQPLPPAAAAARAKESAQDILNLKPLIDKKQWPYVMNDLRLRASYLRYDLKTVISSKATKEEKKDLKDLTGKLFDTLDGVSKTSRQFTYIHSVFTANLIVFTRISGLTCVRLLLFSSVFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DLF6</accession>
    <name>A0A2K2DLF6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g27503v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DLF6"/>
    <dbReference type="EnsemblPlants" id="PNT75104">
        <property type="protein sequence ID" value="PNT75104"/>
        <property type="gene ID" value="BRADI_1g27503v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75104">
        <property type="protein sequence ID" value="PNT75104"/>
        <property type="gene ID" value="BRADI_1g27503v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DLF6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="69" mass="7718" checksum="9CE12AE91ADCE714" modified="2018-03-28" version="1">MIKSKMIANIVQFHYLSQCFFCVALRYCFIIHILSGPSCSCCGIMCILIGRRCTFCFIVGCKGSLHACL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DQN9</accession>
    <name>A0A2K2DQN9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g50115v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DQN9"/>
    <dbReference type="EnsemblPlants" id="PNT76599">
        <property type="protein sequence ID" value="PNT76599"/>
        <property type="gene ID" value="BRADI_1g50115v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76599">
        <property type="protein sequence ID" value="PNT76599"/>
        <property type="gene ID" value="BRADI_1g50115v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DQN9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="250" mass="27052" checksum="972ED5C99EB3F465" modified="2018-03-28" version="1">MRPLPVNSSLVSLSEPAPAPPPVRRPRRRPPPAWRWRSPPSPRRRPYQFIENNIFSCSIPTLARSCGSDTIVGSTTGCEDGSSSSLPSPSPAPKAVMGKGIQPNFILTLLILAGRAADGGRVVVMRRNTASRRCLLISGPVEHNDEDRDRGSPHSGPRGRRQRRCRARRATTMEPGQLLISVAAGAAPARWPRPRTRARGKAASPDLRAAEGAREAVCGGGGRSGRGLTRWWPREWSPCAAPTEKSGWAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DV29</accession>
    <name>A0A2K2DV29_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g74316v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DV29"/>
    <dbReference type="EnsemblPlants" id="PNT78139">
        <property type="protein sequence ID" value="PNT78139"/>
        <property type="gene ID" value="BRADI_1g74316v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78139">
        <property type="protein sequence ID" value="PNT78139"/>
        <property type="gene ID" value="BRADI_1g74316v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DV29"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT78139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="152" mass="16896" checksum="8EA137AB216158C6" modified="2018-03-28" version="1">MDFFYWPIWIGVWSWTTHQGRGCAVGFVSSRTAATASPSPSSPLPAYSCYASCRPSSRPLDLRAAMFNLLADDWLLASRAHLDDAAALGSFLLPRVLLFGFSRLHPLAPSPRALPAGLFFLFFSFSVTFFLGQRFIPNYYSYSLNKLVPAKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPB2</accession>
    <name>I1GPB2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100827633</name>
        <name evidence="5" type="ORF">BRADI_1g11700v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560933.1">
        <property type="nucleotide sequence ID" value="XM_003560885.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPB2"/>
    <dbReference type="FunCoup" id="I1GPB2">
        <property type="interactions" value="1721"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GPB2"/>
    <dbReference type="EnsemblPlants" id="KQK13664">
        <property type="protein sequence ID" value="KQK13664"/>
        <property type="gene ID" value="BRADI_1g11700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827633"/>
    <dbReference type="Gramene" id="KQK13664">
        <property type="protein sequence ID" value="KQK13664"/>
        <property type="gene ID" value="BRADI_1g11700v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827633"/>
    <dbReference type="eggNOG" id="KOG3234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_7_1_1"/>
    <dbReference type="OMA" id="DAHDMRK"/>
    <dbReference type="OrthoDB" id="10264728at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000034">
        <property type="entry name" value="N-alpha-acetyltransferase 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051646">
        <property type="entry name" value="NatB_acetyltransferase_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK13664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="174" mass="20351" checksum="BCEAB6F4CDD0812B" modified="2012-06-13" version="1">MTTIRRFCCDDLLRFASVNLDHLTETFNMSFYMTYMARWPDYFHAAVNPGDRVMGYIMGKVEGQGESWHGHVTAVSVASEFRRQKLAKTLMNLLEEISDKMDKAYFVDLFVRASNMPAIRMYEKLGYVVYRRVLRYYSGEEDGLDMRKALSQDVDKKSIIPLKRPITPDELEYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSY7</accession>
    <name>I1GSY7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100841994</name>
        <name evidence="10" type="ORF">BRADI_1g23320v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560032.1">
        <property type="nucleotide sequence ID" value="XM_003559984.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSY7"/>
    <dbReference type="FunCoup" id="I1GSY7">
        <property type="interactions" value="898"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GSY7"/>
    <dbReference type="EnsemblPlants" id="KQK15505">
        <property type="protein sequence ID" value="KQK15505"/>
        <property type="gene ID" value="BRADI_1g23320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841994"/>
    <dbReference type="Gramene" id="KQK15505">
        <property type="protein sequence ID" value="KQK15505"/>
        <property type="gene ID" value="BRADI_1g23320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841994"/>
    <dbReference type="eggNOG" id="KOG0584">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="OMA" id="YRKVTNV"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000075">
        <property type="entry name" value="Probable serine/threonine-protein kinase WNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000046">
        <property type="entry name" value="probable serine/threonine-protein kinase WNK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK15505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="290" mass="32706" checksum="26722DCB11A4DB82" modified="2012-06-13" version="1">MAADDEEAFEEVDPTGRYGRYADVLGLGSVKKVYRAFDQEEGIEVAWNRVRLRALADRDPGMVDRLHAEVRLLRSLSHDHIIGFHKVWLDRDAGVLSFITEVCNSGSLREYRARHRHVSVKALKKWARQILLGLHHLHTHDPCIIHRDLNCSNVFINGNTGQVKIGDLGLAAIVDKTHVAHTILGTPEFMAPELYTETYTESVDIYSYGMCVLEMVTREMPYAECESVVQIYHSVTRGVPPAALRRLKDPELRGFIDRCIGQPRNRPTAAELLLDPFFSGIDDDTTGTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1GUW0</accession>
    <name>I1GUW0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100822204</name>
        <name evidence="3" type="ORF">BRADI_1g28930v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563188.1">
        <property type="nucleotide sequence ID" value="XM_003563140.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GUW0"/>
    <dbReference type="EnsemblPlants" id="KQK16472">
        <property type="protein sequence ID" value="KQK16472"/>
        <property type="gene ID" value="BRADI_1g28930v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822204"/>
    <dbReference type="Gramene" id="KQK16472">
        <property type="protein sequence ID" value="KQK16472"/>
        <property type="gene ID" value="BRADI_1g28930v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822204"/>
    <dbReference type="eggNOG" id="KOG2372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050634_0_0_1"/>
    <dbReference type="OMA" id="ACTNERY"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GUW0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF74">
        <property type="entry name" value="TLD-DOMAIN CONTAINING NUCLEOLAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="154"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK16472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="322" mass="35647" checksum="5F83802AD035DD8D" modified="2012-06-13" version="1">MQAWKEKVADRLARLLADSPPSPASAAVEPQETPFPAEHSTFTNKGSLSSYVMSLLPNSNLGQENSSPCSGSMRPLPPESLPKRWRGNDFSWKDRPLELSEESGSESDRDERTGSSNNNQILQSHRSGDNQNGNEETSTSDCIGAVYYLTEKSTFVSPELFGFFQSSLPGTLKGCHWVLLYSTWKHGISLRTLLRRTENLQGPCLLIVGDMQGAVFGGLLNGPLRPTEKRKYQGTNQTFVFTTIYGEPRLFRPTGANRYYYLCLKDTLAFGGGGNFALRLDEDLIHGTSGSCETFGNSCLAHGPEFELKNVELWGFSHSRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYS0</accession>
    <name>I1GYS0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100841010</name>
        <name evidence="3" type="ORF">BRADI_1g42760v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563909.1">
        <property type="nucleotide sequence ID" value="XM_003563861.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYS0"/>
    <dbReference type="FunCoup" id="I1GYS0">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK18482">
        <property type="protein sequence ID" value="KQK18482"/>
        <property type="gene ID" value="BRADI_1g42760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841010"/>
    <dbReference type="Gramene" id="KQK18482">
        <property type="protein sequence ID" value="KQK18482"/>
        <property type="gene ID" value="BRADI_1g42760v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841010"/>
    <dbReference type="eggNOG" id="ENOG502QQYU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019628_2_0_1"/>
    <dbReference type="OMA" id="LSMKAIM"/>
    <dbReference type="OrthoDB" id="1523883at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GYS0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK18482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="383" mass="43137" checksum="050B92E62068F52F" modified="2012-06-13" version="1">MPAGSDLRMATGNGENSYAANSRLQEKAILETRPVLRKAVEELYTSLPPRSTMVVADLGCSSGPNTLLVVSEVMGAIRAYTDNKNKWEEEEEAQRAIELQFFLNDLPGNDFNLVFRSLEHFENLGVRLGEKEMPPYYVAGLPGSYYRKLFPCGSVHLFHSSYSLMWRSKVPEEISSGTHLNEGNIYIGETSPPAVIELFQEQFQKDFELFLALRSEELVSGGRVLLTFLGRKSEEMMMHGDVSTLFELVAKSLRSLVLKGRVEKEKLDSFNLPYYTPSVKEVKALINENKLFNIEDIRLFESNWDPQDDSEGDVVLDCARSGANVAKCIRAVLEPMIIDHFGEDIVDELFMLYASIVAKHMKKAKAKYPIILVSLKKATGRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1H0Y3</accession>
    <name>I1H0Y3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100836103</name>
        <name evidence="2" type="ORF">BRADI_1g49060v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561089.1">
        <property type="nucleotide sequence ID" value="XM_003561041.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H0Y3"/>
    <dbReference type="FunCoup" id="I1H0Y3">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H0Y3"/>
    <dbReference type="EnsemblPlants" id="KQK19563">
        <property type="protein sequence ID" value="KQK19563"/>
        <property type="gene ID" value="BRADI_1g49060v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836103"/>
    <dbReference type="Gramene" id="KQK19563">
        <property type="protein sequence ID" value="KQK19563"/>
        <property type="gene ID" value="BRADI_1g49060v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836103"/>
    <dbReference type="eggNOG" id="ENOG502QUVF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014546_2_0_1"/>
    <dbReference type="OMA" id="GATQFFN"/>
    <dbReference type="OrthoDB" id="671439at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009805">
        <property type="term" value="P:coumarin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF33">
        <property type="entry name" value="N-HYDROXYCINNAMOYL_BENZOYLTRANSFERASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK19563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="473" mass="50811" checksum="1B015D4C9DACFCB3" modified="2012-06-13" version="1">MDPPPPPQLRVLDTVQLFAPPPHPRGESELSPSPLAPLSGLDADPNVLGVTFRSVRFFPPHPVSLDPLDTLQRAFAAALALFPQLAGSLRVLDRAAAVVVFSGSNAVPLVLAASDGLSVAEAVDADAPDSPLLDLLAPADVDAGVAGPALALQATRFACGGVALGMRVAHALCDGAGATRFLAAAARFARGEPVAPPVWARRELLGPRDPPRVVAPSFDTVLAPDYDAVGPYGGHGHEQQKLLKVCFHVSDVRVETLRARLSAEAAGVRLTTFEVLAAFIWRARVKANGTSPEETVKMVYSMNISKLLDPPLPEGYWGNVCVPVYVSLTSGDLLGRSLAETATLVRKSKREVDDEYVRSYVDFLELRRREGVTATAGRGGVSAFTDWRRLGHGEVDFGWGGPDAVLPLSWRILGSEEPCFLVPYGISDERRRRGFKVFVAVRETAVACFREEMEEILLLQQEERGNYSTAGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1H3A0</accession>
    <name>I1H3A0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100844809</name>
        <name evidence="1" type="ORF">BRADI_1g56010v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557517.1">
        <property type="nucleotide sequence ID" value="XM_003557469.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H3A0"/>
    <dbReference type="STRING" id="15368.I1H3A0"/>
    <dbReference type="EnsemblPlants" id="KQK20679">
        <property type="protein sequence ID" value="KQK20679"/>
        <property type="gene ID" value="BRADI_1g56010v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844809"/>
    <dbReference type="Gramene" id="KQK20679">
        <property type="protein sequence ID" value="KQK20679"/>
        <property type="gene ID" value="BRADI_1g56010v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844809"/>
    <dbReference type="eggNOG" id="ENOG502S2PF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094777_0_0_1"/>
    <dbReference type="OMA" id="HEAPNCF"/>
    <dbReference type="OrthoDB" id="1922291at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00298">
        <property type="entry name" value="ACD_sHsps_p23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33879">
        <property type="entry name" value="17.6 KDA CLASS II HEAT SHOCK PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33879:SF3">
        <property type="entry name" value="17.6 KDA CLASS II HEAT SHOCK PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK20679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="17340" checksum="5672A4F91FA50FE3" modified="2012-06-13" version="1">MRVHPAPRKRTIAVQRCAAAAADGAKKLRRLPHIFARVLELPFAADADVAVEEDAAALRFVAAADGFSPGGARAHAVQIHPGVTKVVVRGLSVAGAGEDDDDGAAAFELDRWRFRLPPCTRPTLATATYAEGELVVTVPKGAAPDESDGDGAHILGSAGADNVLLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4N1</accession>
    <name>I1H4N1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GBF-interacting protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100821170</name>
        <name evidence="2" type="ORF">BRADI_1g60080v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228510.1">
        <property type="nucleotide sequence ID" value="XM_010230208.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H4N1"/>
    <dbReference type="FunCoup" id="I1H4N1">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H4N1"/>
    <dbReference type="EnsemblPlants" id="KQK21307">
        <property type="protein sequence ID" value="KQK21307"/>
        <property type="gene ID" value="BRADI_1g60080v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821170"/>
    <dbReference type="Gramene" id="KQK21307">
        <property type="protein sequence ID" value="KQK21307"/>
        <property type="gene ID" value="BRADI_1g60080v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821170"/>
    <dbReference type="eggNOG" id="ENOG502QR20">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018374_1_0_1"/>
    <dbReference type="OMA" id="PQMYQHM"/>
    <dbReference type="OrthoDB" id="657470at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H4N1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47070:SF3">
        <property type="entry name" value="GLYCOPROTEIN FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47070">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK21307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="797" mass="86386" checksum="543F0A1D2918200A" modified="2012-06-13" version="1">MDGATATATDAGRPLPRGSSCDVRNRQENYKENTSYQRSNDIRAHKERQSEWTKPGRVFNRNISRGGYSRSSFSGVTQEFRVVKDNRIKQKADGDTVPGSFHNGDSSSEHTVTNIGDKSSTEKLSDGHGTTQADNGHKVAAQAHGKEVKLSSDQRLEQSERMRTPLVGSHALQEKGKQNKELAAPSVTNNFTQELCCSSSDPIHVPPPGSRPVGTFGAIKREVGVVGARQRPSDIAATNISTSNSLATVTMVMKDNASSEHQSRLSVVPSKTSHSSSSVSLSSRPFLPSQVYSKPSIHVGHSKGNPHLEWKPKSVRPTSVNCAANVAPSSATSSADVHQVGMACLSKKLSQANVSKDERVIMPEHLKVPDNERTHLIFGTFESEAKSNASTSSPIATSKEGLDVHSSTRLTPLNSVRSSDEVRPGDQTDHVGSCATLPQSDVLSFSEHQQSSIVDIGPSPGVIGEYRTNEMISNKIAHSVPQPQYQDNYAVQNFKAYEPDSKYEMPFITKSIDGETVQTRSYPSEIMGLHPSNVNQLSVSSASQQPVPQMYQQVQVPQYPSFLPYRHVFSPYYVPPVAVPNYSSNPSFPQLPHASNYLVMPNGASHEIGSMKFGTPHQYKQVFPGSPAGYGSYANQSGYPVSNCIIGSTGAVEDASMSKYKDNNNMYTPNQQAETTDLWIQAHRDIPSMPTTPFYNMMGRPMSPHTAYLPAHNGHAPFNPAPHPAHLQFPGLPHPLQPTSMTMVQNPQSMVHQPAGNMGIDMAAMAPGAQAGAFPGAQVGAFQQNQLGHLGWAPPNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4V4</accession>
    <name>I1H4V4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100821742</name>
        <name evidence="12" type="ORF">BRADI_1g60700v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+) = (S)-allantoin + CO2</text>
            <dbReference type="Rhea" id="RHEA:26301"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15678"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="4.1.1.97"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="3.5.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; urate degradation; (S)-allantoin from urate: step 3/3.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557779.1">
        <property type="nucleotide sequence ID" value="XM_003557731.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H4V4"/>
    <dbReference type="FunCoup" id="I1H4V4">
        <property type="interactions" value="1016"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H4V4"/>
    <dbReference type="EnsemblPlants" id="KQK21430">
        <property type="protein sequence ID" value="KQK21430"/>
        <property type="gene ID" value="BRADI_1g60700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821742"/>
    <dbReference type="Gramene" id="KQK21430">
        <property type="protein sequence ID" value="KQK21430"/>
        <property type="gene ID" value="BRADI_1g60700v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821742"/>
    <dbReference type="eggNOG" id="KOG3006">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="EPEGHYH"/>
    <dbReference type="OrthoDB" id="10265230at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H4V4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031234">
        <property type="term" value="C:extrinsic component of cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051997">
        <property type="term" value="F:2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033971">
        <property type="term" value="F:hydroxyisourate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019428">
        <property type="term" value="P:allantoin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009631">
        <property type="term" value="P:cold acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900457">
        <property type="term" value="P:regulation of brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001560">
        <property type="term" value="P:regulation of cell growth by extracellular stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900140">
        <property type="term" value="P:regulation of seedling development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd05822">
        <property type="entry name" value="TLP_HIUase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3330.10:FF:000002">
        <property type="entry name" value="Uric acid degradation bifunctional protein TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.180:FF:000003">
        <property type="entry name" value="Uric acid degradation bifunctional protein TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3330.10">
        <property type="entry name" value="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="entry name" value="Transthyretin/hydroxyisourate hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014306">
        <property type="entry name" value="Hydroxyisourate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018020">
        <property type="entry name" value="OHCU_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036778">
        <property type="entry name" value="OHCU_decarboxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023418">
        <property type="entry name" value="Thyroxine_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000895">
        <property type="entry name" value="Transthyretin/HIU_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02962">
        <property type="entry name" value="hdxy_isourate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43466">
        <property type="entry name" value="2-OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43466:SF1">
        <property type="entry name" value="2-OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09349">
        <property type="entry name" value="OHCU_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00189">
        <property type="entry name" value="TRNSTHYRETIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="Transthyretin (synonym: prealbumin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158694">
        <property type="entry name" value="UraD-Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00768">
        <property type="entry name" value="TRANSTHYRETIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase" evidence="11">
        <location>
            <begin position="8"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Transthyretin/hydroxyisourate hydrolase" evidence="10">
        <location>
            <begin position="213"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600895-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK21430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="332" mass="36148" checksum="6677D5F79584D272" modified="2012-06-13" version="1">MATPTPLAVEDVLRVNGSRRFAAALAAASPFASLADAVLAARRIWLDKVDVTGWLEAFAAHPPIGSTSPSVSKWSQEEQSAALSTATDSAAQELAEWNARYREKFGFVFMICASGRTAPEVLSELKRRYTNRPIVELEVAAQEELKITELRLAKLFSSETPAPFTSAEGHTSQSDKAADRMRIIGAHLGALSQPSTNKAPEITGSSNRTRPPITTHVLDTARGSPASGIEVSLEVWKDASSRPSFDNKDFNGWTTLGSSVTNNDGRSGQLMDIVDNVTPGFYRISFNTSKYAPSGFFPYVSIVFEIKKSQTTEHFHVPLLHSPFSFTTYRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HEV4</accession>
    <name>I1HEV4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">mRNA export factor GLE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Nucleoporin GLE1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100832549</name>
        <name evidence="12" type="ORF">BRADI_2g11627v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230825.1">
        <property type="nucleotide sequence ID" value="XM_010232523.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HEV4"/>
    <dbReference type="STRING" id="15368.I1HEV4"/>
    <dbReference type="EnsemblPlants" id="KQK04090">
        <property type="protein sequence ID" value="KQK04090"/>
        <property type="gene ID" value="BRADI_2g11627v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK04091">
        <property type="protein sequence ID" value="KQK04091"/>
        <property type="gene ID" value="BRADI_2g11627v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832549"/>
    <dbReference type="Gramene" id="KQK04090">
        <property type="protein sequence ID" value="KQK04090"/>
        <property type="gene ID" value="BRADI_2g11627v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04091">
        <property type="protein sequence ID" value="KQK04091"/>
        <property type="gene ID" value="BRADI_2g11627v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832549"/>
    <dbReference type="eggNOG" id="KOG2412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020707_0_0_1"/>
    <dbReference type="OMA" id="VPANIHS"/>
    <dbReference type="OrthoDB" id="420884at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HEV4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.510:FF:000002">
        <property type="entry name" value="Protein GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.510">
        <property type="entry name" value="GLE1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012476">
        <property type="entry name" value="GLE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038506">
        <property type="entry name" value="GLE1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960">
        <property type="entry name" value="GLE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960:SF0">
        <property type="entry name" value="MRNA EXPORT FACTOR GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07817">
        <property type="entry name" value="GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK04091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="606" mass="68648" checksum="D6DC3A5BD36AF30E" modified="2012-06-13" version="1">MAFTRVELRCPKMLDPCPSWTLGDVLTEFDAFEATRLAAPPMPLKQPPKWADGGSGREKAFVMRVEEEGDTEDEDDDSDEDARALVAHGTSFSCNDIESSDSEESGDELDGRVTPYHLMEKRSLEKSILLELEREHHLKIQEEVRSKLSALEVCHQNEIQRTISAFARLQKYAESRKEIDRRLDVHFQRKIAEILDKHLSMVQRDHEQKSQIVERRIRDDAAVEEAKRREQAMKEDKIKQERTRQEAETRQKAAAKLAAEAQKAAYEAAQKEAAETEAAKLRAAAVSQNSVAHATTVNKKEIKSELPGIKVFADSSALEAESRRHALHDQVPSNIYLSKEFSKYDRQIGKSISKLMPTTDSVKARASELVKALDGHECPRPIACRLFADKIISIVKSRNPKDKTFGNLAFACGYVMLLVTNQVPEAMDYLLAEFHKVCVYTVPKHLHALNAQARNRDYYRLIGYQEENGQLESTEKYLTNVAAYVKLYAAMIQTEIKGVHHPHGLAEGWKWLAMFLNTLPATTATACALHAFLKMAGFALHKKYGSQFMKVLDVISRCFLPVLKEQGSKVQPEVVNNLQNYLNDKIYLEEPEGQYLAQQLLSKVFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1HGN3</accession>
    <name>I1HGN3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100833175</name>
        <name evidence="10" type="ORF">BRADI_2g17220v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCNDBP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567933.1">
        <property type="nucleotide sequence ID" value="XM_003567885.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HGN3"/>
    <dbReference type="FunCoup" id="I1HGN3">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HGN3"/>
    <dbReference type="EnsemblPlants" id="KQK04989">
        <property type="protein sequence ID" value="KQK04989"/>
        <property type="gene ID" value="BRADI_2g17220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833175"/>
    <dbReference type="Gramene" id="KQK04989">
        <property type="protein sequence ID" value="KQK04989"/>
        <property type="gene ID" value="BRADI_2g17220v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833175"/>
    <dbReference type="eggNOG" id="ENOG502SGCW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020897_0_0_1"/>
    <dbReference type="OMA" id="QATTVGM"/>
    <dbReference type="OrthoDB" id="41588at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026907">
        <property type="entry name" value="GCIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049317">
        <property type="entry name" value="GCIP-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049318">
        <property type="entry name" value="GCIP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492">
        <property type="entry name" value="CYCLIN D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492:SF1">
        <property type="entry name" value="CYCLIN-D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20936">
        <property type="entry name" value="GCIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13324">
        <property type="entry name" value="GCIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like N-terminal" evidence="8">
        <location>
            <begin position="52"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like C-terminal" evidence="9">
        <location>
            <begin position="217"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK04989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="371" mass="39960" checksum="14EEA67442AE2EFD" modified="2012-06-13" version="1">MSKKGGRKKTAAAGAGDELSRFLQPHLQTISDTLQLMADAAPSGLERTEWSEVVQLGDLVYRQATVAGMVWSGDLPDVETLKENIVAYFNVLQGFLLACHGSTVGAGPTLHKYISSFAKSVVDSSISLFKLAVSAYESGKPDRKTIIPPVTGTVWEACLALKKVPTTNCTAIGRAMTQICLCLKDILREMKELPIGDSGDITAEKSSNGDVDISSDKDESSSDLEVDDEFSEEEIAIAKMITAVTSDSLVVVKEAIRFITSLLKSSGNQRDASEDKVESMENLLSCCREIAEQINDLGASVYTQETSEMKSAIKGLYNGINGMRKEIGDLGGSPENIFAAFGGFEKSLGALEEKIDEDVVNEMENLTICRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1HIK6</accession>
    <name>I1HIK6_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100830729</name>
        <name evidence="1" type="ORF">BRADI_2g22780v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568271.1">
        <property type="nucleotide sequence ID" value="XM_003568223.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HIK6"/>
    <dbReference type="STRING" id="15368.I1HIK6"/>
    <dbReference type="EnsemblPlants" id="KQK05828">
        <property type="protein sequence ID" value="KQK05828"/>
        <property type="gene ID" value="BRADI_2g22780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830729"/>
    <dbReference type="Gramene" id="KQK05828">
        <property type="protein sequence ID" value="KQK05828"/>
        <property type="gene ID" value="BRADI_2g22780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830729"/>
    <dbReference type="eggNOG" id="ENOG502RZYC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106028_0_0_1"/>
    <dbReference type="OMA" id="KHIGNAY"/>
    <dbReference type="OrthoDB" id="1907500at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045282">
        <property type="entry name" value="At4g08330-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33674:SF14">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33674">
        <property type="entry name" value="METHIONINE-S-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24046">
        <property type="entry name" value="At4g08330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK05828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="147" mass="16399" checksum="E1BBEE9DF5C4BBB0" modified="2012-06-13" version="1">MARTLDGCYSTNTKDVAYSCGYCGYALNLSSSTRNTANIGSKYGKQIRKGVVSFFAIDESRFTQTDEVSCMPYFRSRCSWGFFRKRTRLLCRKCGGHIGDSYEDKDSPLYDSLDDTHLSSAGSRKKYVIKINALQPSSDDSGVPFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJK6</accession>
    <name>I1HJK6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100821303</name>
        <name evidence="4" type="ORF">BRADI_2g25837v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568493.1">
        <property type="nucleotide sequence ID" value="XM_003568445.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJK6"/>
    <dbReference type="STRING" id="15368.I1HJK6"/>
    <dbReference type="EnsemblPlants" id="KQK06341">
        <property type="protein sequence ID" value="KQK06341"/>
        <property type="gene ID" value="BRADI_2g25837v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821303"/>
    <dbReference type="Gramene" id="KQK06341">
        <property type="protein sequence ID" value="KQK06341"/>
        <property type="gene ID" value="BRADI_2g25837v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821303"/>
    <dbReference type="eggNOG" id="KOG1267">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034145_1_1_1"/>
    <dbReference type="OMA" id="MVSLDNC"/>
    <dbReference type="OrthoDB" id="637682at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000001">
        <property type="entry name" value="Mitochondrial transcription termination factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF93">
        <property type="entry name" value="OS05G0403600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK06341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="394" mass="43726" checksum="37A5B8C0A45334DD" modified="2012-06-13" version="1">MFPSICRRRLVFLIRQIPGDGTSLFQSIPDAIPLARSYSSTAVAGAPNSALCSAAATASYLVSCGFSTADAATLTTARNFRIRSPEKADAARALLRSYGFTDADIVRMARSYSMILNADPERIIRPKLDFFAALGFEPGKLATAPFVLARSLDKHIVPCIQFLRGIIASDDLIRLGFSRCPRALMVDPENNMRPAVEALRRCGLPDAAISKLLVIHMGVLMLSPDRIIQIFQDLKAIDMCVEDSRFLYCFRVMSSVKRETWLRKLALYKSLGLSEGEVIKAFKTQPTILLSADETIKKKVRFFVDELKLEISDIVERAVTLAYSMEKCILPRCAVLSVLMKEGKIQRDINLLPALLGSSRAFSARFVSRYADDVPDVVKAYEGKIKFEGFGYHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>I1HKG2</accession>
    <name>I1HKG2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100845690</name>
        <name evidence="9" type="ORF">BRADI_2g29960v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568656.1">
        <property type="nucleotide sequence ID" value="XM_003568608.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HKG2"/>
    <dbReference type="FunCoup" id="I1HKG2">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HKG2"/>
    <dbReference type="EnsemblPlants" id="KQK06814">
        <property type="protein sequence ID" value="KQK06814"/>
        <property type="gene ID" value="BRADI_2g29960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845690"/>
    <dbReference type="Gramene" id="KQK06814">
        <property type="protein sequence ID" value="KQK06814"/>
        <property type="gene ID" value="BRADI_2g29960v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTBU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="PTCISED"/>
    <dbReference type="OrthoDB" id="550883at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HKG2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31241:SF32">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31241">
        <property type="entry name" value="DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="131"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="53"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="237"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK06814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="381" mass="41651" checksum="6571C84A206E665D" modified="2012-06-13" version="1">MTVDQRSVAAAPLEIPALQPGRTLGAEANTRSHVSVESIGSCTLPCNECELSAQQTPKGVAPVASILRKKRPRRSRDGPNSVSETIRRWKEVNQQLEHDPEGAKRARKPPAKGSKKGCMQGKGGPENTRCKFRGVRQRTWGKWVAEIREPNRVSRLWLGTFPTAETAACAYDEAARAMYGPLARTNFTIQDVPTPAVDIPAVVQRLLPGGSTSCESTMTSNHSGIVASSRVLEISSSLKQSDVGSEHDQRSNQYSSPQAGSSVARSRADDLFEPLEPIANLPDGEDDGFDIEELLRMMEADPVEAAEPMVENSWTGFQDVGANTVVDFDQQEPSYLDDFNPSMLEGMLQLAEPFPTCISEDRVMFNPGLRDADLSEFFEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HKP0</accession>
    <name>I1HKP0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100831950</name>
        <name evidence="2" type="ORF">BRADI_2g31270v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568695.1">
        <property type="nucleotide sequence ID" value="XM_003568647.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HKP0"/>
    <dbReference type="FunCoup" id="I1HKP0">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HKP0"/>
    <dbReference type="EnsemblPlants" id="KQK06917">
        <property type="protein sequence ID" value="KQK06917"/>
        <property type="gene ID" value="BRADI_2g31270v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831950"/>
    <dbReference type="Gramene" id="KQK06917">
        <property type="protein sequence ID" value="KQK06917"/>
        <property type="gene ID" value="BRADI_2g31270v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831950"/>
    <dbReference type="eggNOG" id="KOG2813">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044550_1_0_1"/>
    <dbReference type="OMA" id="KRCNTCS"/>
    <dbReference type="OrthoDB" id="3355217at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052789">
        <property type="entry name" value="SSUH2_homolog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48465">
        <property type="entry name" value="PROTEIN SSUH2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48465:SF1">
        <property type="entry name" value="PROTEIN SSUH2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="49790" checksum="3FBEEAA941E41650" modified="2012-06-13" version="1">MTMEAEREGEEQRRPLISSASAPAAEHQQQYQHLGRSSSSALRGGGGGWGGGGPEVSAAEVKSAASFSSNHYPPAPDPNNQDVYPPSIHSAVLYQSPSPAPTPPHPHGLAIVPGGPYPYGGDYQPSQGVTRDVLDEVEIRNLLIEHVGHRCCWASRPARTWKITSIEDCNVYVGTLETFIEERDIITKKEPYDGGKIDGRHKGPVLGVWELDLRSEFPLLFVPEKEVMVKIPHSEVTEKCLDCESRGEIPCPTCNAGQQPGFYKANQMTRCSACHGRGLLARQDGSDTVCGMCNGQGMLPCIACGSRGLVTCKTCNGCGSLVAQSTAHVRWKTLSARKVSATTGAASVPEEVFHRAKGVQLCNIQAYQCTPAFFADSYPLNQFSSEVVASRLPVPPSARVISERHIISVVPVTRVTMAHRKRSFSFYVVGYSRDVFVRDYPSKFCWGLCCCFEWLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HL59</accession>
    <name>I1HL59_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100834312</name>
        <name evidence="2" type="ORF">BRADI_2g33500v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568789.2">
        <property type="nucleotide sequence ID" value="XM_003568741.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HL59"/>
    <dbReference type="STRING" id="15368.I1HL59"/>
    <dbReference type="EnsemblPlants" id="KQK07168">
        <property type="protein sequence ID" value="KQK07168"/>
        <property type="gene ID" value="BRADI_2g33500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834312"/>
    <dbReference type="Gramene" id="KQK07168">
        <property type="protein sequence ID" value="KQK07168"/>
        <property type="gene ID" value="BRADI_2g33500v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834312"/>
    <dbReference type="eggNOG" id="ENOG502RKPE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111343_1_0_1"/>
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    <dbReference type="OrthoDB" id="5371818at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd07245">
        <property type="entry name" value="VOC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
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        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050383">
        <property type="entry name" value="GlyoxalaseI/FosfomycinResist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366">
        <property type="entry name" value="GLYOXALASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366:SF22">
        <property type="entry name" value="VOC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="80"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK07168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="199" mass="21725" checksum="EC47E88A02B6C307" modified="2012-06-13" version="1">MHRTAAKDLRQAAMSTRCISSLALLSPSGSGKSCAVVRAHPALSSAARSRIRTHMSVATGSEQALTAQEQSQEPEYGVVSIHHVGILCENLERSIAFYQDLLGLKVNPARPTDKLPYRGAWLWVGSEMIHLMELPNPDPLTGRPEHGGRDRHTCIAIKDVLKLKEIFDKAGISYTLSKSGRPAIFARDPDGNALEFTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HLM1</accession>
    <name>I1HLM1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100845583</name>
        <name evidence="7" type="ORF">BRADI_2g35057v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566530.1">
        <property type="nucleotide sequence ID" value="XM_003566482.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HLM1"/>
    <dbReference type="FunCoup" id="I1HLM1">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK07398">
        <property type="protein sequence ID" value="KQK07398"/>
        <property type="gene ID" value="BRADI_2g35057v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845583"/>
    <dbReference type="Gramene" id="KQK07398">
        <property type="protein sequence ID" value="KQK07398"/>
        <property type="gene ID" value="BRADI_2g35057v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845583"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060197_2_0_1"/>
    <dbReference type="OMA" id="VWMKMGE"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000132">
        <property type="entry name" value="C2C2-GATA transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052138">
        <property type="entry name" value="GATA_ZnFinger_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47255">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47255:SF4">
        <property type="entry name" value="GATA ZINC FINGER DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="6">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="57"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK07398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="154" mass="17076" checksum="A9849021AE96F4EB" modified="2012-06-13" version="1">MDSPHHKAIGVAAAAAAEGGRMCCVECRTTTTPMWRSGPTGPRSLCNACGIRYRKKRRQELGLDNKQLQSQPQQQQEQQQHQQQEQQQQQQEQQQHQQQEDHSEPTSAVKDSSSSPSKTSKLQVVKKRRVSMGVEEAASLLMALSSSSTPTLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1HMU2</accession>
    <accession>A0A0Q3MU96</accession>
    <name>I1HMU2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pectin acetylesterase</fullName>
            <ecNumber evidence="5">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100837473</name>
        <name evidence="6" type="ORF">BRADI_2g38780v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Secreted</location>
            <location evidence="2 5">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the pectinacetylesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07934.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566691.3">
        <property type="nucleotide sequence ID" value="XM_003566643.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HMU2"/>
    <dbReference type="STRING" id="15368.I1HMU2"/>
    <dbReference type="ESTHER" id="bradi-i1hmu2">
        <property type="family name" value="Pectinacetylesterase-Notum"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK07934">
        <property type="protein sequence ID" value="KQK07934"/>
        <property type="gene ID" value="BRADI_2g38780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837473"/>
    <dbReference type="Gramene" id="KQK07934">
        <property type="protein sequence ID" value="KQK07934"/>
        <property type="gene ID" value="BRADI_2g38780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837473"/>
    <dbReference type="eggNOG" id="KOG4287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031008_0_0_1"/>
    <dbReference type="OMA" id="CNDACES"/>
    <dbReference type="OrthoDB" id="2015280at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HMU2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF83">
        <property type="entry name" value="PECTIN ACETYLESTERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK07934.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="437" mass="48915" checksum="AAC37C814D869A70" modified="2012-06-13" version="1">MSPSSAPASRRHLRLWWRRRGRAGAVAATFAAALLAAGLLLALSYYASVVAPRAAPSSSSSSSGALVDLTLLRRAEKKGALCLDGSAPGYHLQRGSGSGSQSWLIHLEGGGWCRNLKSCASRQKSMLGSSRYMERQVEFAGILSDDEAQNPDFYDWNKVKIRYCDGASFSGNVKDEFQNGTKFFFRGQRIWKAVMDELLLKGLKHAKQAFLTGCSAGGLATYIHCDDFRALLPKDSRVKCLADGGFFLDVEDISKQRTLRAFYSEVVRLQDLKRRFLHCSSSEDPGQCFFPREVVKAIHTPVFVLNPAYDAWQVQHVLAPEASDPKHSWLDCRLDISKCNPNQLKILQGFREELHVAMSELKQKKDWGIFIDSCFVHCQSLNSLTWHSPSSPRVSNKTIAEAVGDWFFDRREVKELDCEYPCNPTCHNLVFDKPFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPT1</accession>
    <name>I1HPT1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1645 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100822333</name>
        <name evidence="2" type="ORF">BRADI_2g44840v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569426.1">
        <property type="nucleotide sequence ID" value="XM_003569378.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPT1"/>
    <dbReference type="FunCoup" id="I1HPT1">
        <property type="interactions" value="1278"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HPT1"/>
    <dbReference type="EnsemblPlants" id="KQK08908">
        <property type="protein sequence ID" value="KQK08908"/>
        <property type="gene ID" value="BRADI_2g44840v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822333"/>
    <dbReference type="Gramene" id="KQK08908">
        <property type="protein sequence ID" value="KQK08908"/>
        <property type="gene ID" value="BRADI_2g44840v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822333"/>
    <dbReference type="eggNOG" id="ENOG502RXG8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059090_2_0_1"/>
    <dbReference type="OMA" id="PWRTRSK"/>
    <dbReference type="OrthoDB" id="666789at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012442">
        <property type="entry name" value="DUF1645_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095:SF130">
        <property type="entry name" value="OS01G0639600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095">
        <property type="entry name" value="OS07G0619500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07816">
        <property type="entry name" value="DUF1645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK08908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="27203" checksum="8BBF3EFD246F73A1" modified="2012-06-13" version="1">MEAEPRSDRLRQEPTVATRENGGAVNNSGDESDGEFEFSFASRPDPAAATGGATADELFYPVFGRVFDETRAPVRRPLGKLFLEESAPARNSSVGSTSSSSSSAATDATDLDGVSPDTYCVWTPGAPSPASSPARSPRKSGSTGSLSRWRRVSELLVIGRSHSEGRDKFRFLSAPPSPARDHQPKAKPSQRGAVNAKAATELDTVAASHRMFYGSPKGSASPGAARRTFLPYRQDLVGLFSNAKGLSRSPYYQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUV2</accession>
    <name>I1HUV2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucosidebeta-D-glucosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_2g59660v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUV2"/>
    <dbReference type="FunCoup" id="I1HUV2">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HUV2"/>
    <dbReference type="EnsemblPlants" id="KQK11357">
        <property type="protein sequence ID" value="KQK11357"/>
        <property type="gene ID" value="BRADI_2g59660v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11357">
        <property type="protein sequence ID" value="KQK11357"/>
        <property type="gene ID" value="BRADI_2g59660v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0626">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001859_1_0_1"/>
    <dbReference type="InParanoid" id="I1HUV2"/>
    <dbReference type="OMA" id="TECGYAN"/>
    <dbReference type="OrthoDB" id="65569at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000069">
        <property type="entry name" value="Beta-glucosidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF335">
        <property type="entry name" value="BETA-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094805" description="4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucosidebeta-D-glucosidase" evidence="6">
        <location>
            <begin position="20"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK11357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="56744" checksum="B79E70D414473D1F" modified="2012-06-13" version="1">MAILSSLLLLLLLLSAAHGAAAPAPDGYTRSDFPRGFVFGAATSAYQYEGAVAEDGRSPSIWDTFTHAGKTPDKSVGDVAADGYHKYKDDVKLMAETNLEAYRFSISWSRLIPNGRGAVNPKGLEYYNNLIDELVKHGIQIHVMLHQLDYPQILDDEYGGWLSTRIVEDFTAFADVCFGEFGDRVSYWTTIDEPNVAAVGSYDTAQIAPGRCSDPFGSTKCTAGDSTVEPYVAAHNMILAHASATRLYRGKYQALQKGVVGINIYTFWTYPLTNSTADIEATKRCRDFMFNWILEPLVFGDYPQVMKKIVGSRLPSFTKVQSEAVKGSVDFIGINHYYTLYVNDSPLQKGVRDFALDMSSAYRGSKTDPPVGKYAPTAIPNDPEGLQLMMLYLKETYGDIPIYVQESGHGSGNDTIDDTDRVEYLKTFIESTLDAIKDGANVKGYFVWSFLDVFEVLSGYKSRYGLYRVDFDNEALPRQARLSACWYSGFLRKNNGTHVQSVLGNAGPHAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1HV71</accession>
    <name>I1HV71_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="4 14">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100832429</name>
        <name evidence="17" type="ORF">BRADI_2g60730v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="4 14"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232818.1">
        <property type="nucleotide sequence ID" value="XM_010234516.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HV71"/>
    <dbReference type="STRING" id="15368.I1HV71"/>
    <dbReference type="EnsemblPlants" id="KQK11539">
        <property type="protein sequence ID" value="KQK11539"/>
        <property type="gene ID" value="BRADI_2g60730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832429"/>
    <dbReference type="Gramene" id="KQK11539">
        <property type="protein sequence ID" value="KQK11539"/>
        <property type="gene ID" value="BRADI_2g60730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832429"/>
    <dbReference type="eggNOG" id="KOG3124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_3_1_1"/>
    <dbReference type="OMA" id="VWAVKPQ"/>
    <dbReference type="OrthoDB" id="10263291at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000190">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 14" id="KW-0521">NADP</keyword>
    <keyword evidence="10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 14" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="15">
        <location>
            <begin position="23"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="16">
        <location>
            <begin position="180"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK11539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="287" mass="29539" checksum="B3ECCAE15F6DEA90" modified="2012-06-13" version="1">MAAPPQPSPAAPAPAAAAADAFRLGFVGAGNLAESIARGVAASGVLPASAIRTAPHRRPERGDAFASLGARILASNGQVVDDSDVIVISVKPQIVKQVLVELKPLLSEEKLLVSIAAGIKMKDLQDWSGQRRIIRVMPNTPSAVGQAASVMCLGEKATENDENRVRSLFSAIGKVWTAEEKYFDAVTGLSGSGPAYIFLAIEAMADGGVAAGLPRDLALGLAAQTVLGAATMVSQTGKHPGQLKDQVTSPAGTTIAGVHELEKGSFRGTLINAVVAATTRCRELSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZ75</accession>
    <name>I1HZ75_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100828100</name>
        <name evidence="7" type="ORF">BRADI_3g09340v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Transcription regulator.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572005.1">
        <property type="nucleotide sequence ID" value="XM_003571957.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZ75"/>
    <dbReference type="STRING" id="15368.I1HZ75"/>
    <dbReference type="EnsemblPlants" id="KQJ94231">
        <property type="protein sequence ID" value="KQJ94231"/>
        <property type="gene ID" value="BRADI_3g09340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828100"/>
    <dbReference type="Gramene" id="KQJ94231">
        <property type="protein sequence ID" value="KQJ94231"/>
        <property type="gene ID" value="BRADI_3g09340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828100"/>
    <dbReference type="eggNOG" id="ENOG502QQW5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059253_1_1_1"/>
    <dbReference type="OMA" id="KNDEVHS"/>
    <dbReference type="OrthoDB" id="1435597at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14703">
        <property type="entry name" value="bZIP_plant_RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000057">
        <property type="entry name" value="Basic leucine zipper 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044759">
        <property type="entry name" value="bZIP_RF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052483">
        <property type="entry name" value="bZIP_transcription_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46391:SF3">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46391">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="114"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ94231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="243" mass="26062" checksum="87975F539BC59C17" modified="2012-06-13" version="1">MAQLPPRAPSAAGQQDWSAAGEFLGFAASRRGAHRRSASDSAAFLEAVPMDDDVIGGGEFDRLDDEQLMSMFSDVDAPAVSSDGGGGGFMDMGDGEEGSAGARAMAAAEGGYGDPKRVKRILANRQSAQRSRVRKLQYISELERSVTGLQMEVSALSPRVAFLDHQRSLLTVGNSHLRQRIAALAQDKIFKDAHQEALKEEIERLRQVYHQQQIKATGGADIATAASMQAKQELLACEGAAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZB6</accession>
    <name>I1HZB6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100837792</name>
        <name evidence="9" type="ORF">BRADI_3g09670v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bHLH protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571175.1">
        <property type="nucleotide sequence ID" value="XM_003571127.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZB6"/>
    <dbReference type="FunCoup" id="I1HZB6">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HZB6"/>
    <dbReference type="EnsemblPlants" id="PNT66286">
        <property type="protein sequence ID" value="PNT66286"/>
        <property type="gene ID" value="BRADI_3g09670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837792"/>
    <dbReference type="Gramene" id="PNT66286">
        <property type="protein sequence ID" value="PNT66286"/>
        <property type="gene ID" value="BRADI_3g09670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837792"/>
    <dbReference type="eggNOG" id="ENOG502QS6Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044652_0_0_1"/>
    <dbReference type="OMA" id="QTRMSHI"/>
    <dbReference type="OrthoDB" id="675169at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010052">
        <property type="term" value="P:guard cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010374">
        <property type="term" value="P:stomatal complex development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02116">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054502">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044283">
        <property type="entry name" value="FAMA/SPEECHLESS/MUTE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46684">
        <property type="entry name" value="TRANSCRIPTION FACTOR FAMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46684:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPEECHLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22754">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="114"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="73"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT66286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="376" mass="39770" checksum="7E641D65669807FC" modified="2012-06-13" version="1">MAMGDDGGLCKLFANDDCEFRHVSGDLFSILDTWEKCMNGAGEGGGGAPCSAAALSPSSVVVVDGAAENNAVVGVRPKPGSRRREAADEEKGGAPGRKKHKGSTVVDDGSDGAAKMSSHITVERNRRKQMNENLAVLRTLMPCFYVKRGDQASVIGGVVDYIKELQQVLHSLEAKKHRKVYAVEHALSPRPGPTPLSPRPLLKPPISPRPAVPISPPTPTPGSPYKPIQRLPHYISPATSSSSTISHAASYDVISPRPYLPTLDSIITELAAQAAARAPGALGGGGGGAGLNLLLLPDVKVEFAGPNLVLKTTSHRARPGQVLRIIAALESLSLEILHVSISTVDDTMVHSFTIKIGIECELSAEELVQEIRQTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1E3</accession>
    <name>I1I1E3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tobamovirus multiplication protein 2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100836568</name>
        <name evidence="6" type="ORF">BRADI_3g16410v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573463.1">
        <property type="nucleotide sequence ID" value="XM_003573415.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1E3"/>
    <dbReference type="STRING" id="15368.I1I1E3"/>
    <dbReference type="EnsemblPlants" id="KQJ95310">
        <property type="protein sequence ID" value="KQJ95310"/>
        <property type="gene ID" value="BRADI_3g16410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836568"/>
    <dbReference type="Gramene" id="KQJ95310">
        <property type="protein sequence ID" value="KQJ95310"/>
        <property type="gene ID" value="BRADI_3g16410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836568"/>
    <dbReference type="eggNOG" id="ENOG502QRDH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055730_0_0_1"/>
    <dbReference type="OMA" id="SAWFIFT"/>
    <dbReference type="OrthoDB" id="432835at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF425">
        <property type="entry name" value="OS08G0159100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ95310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="30681" checksum="ED5C19FED7B96F96" modified="2012-06-13" version="1">MACRGFFECVLKLLNFVVMVVGLAMAGYGAYLLVLWLQVLSPPLPPSPAPPAAVAPSGGDLVRLGRPMLLLLDASLSDNGTVERLSSAWFIFAFIGIGVILFITSIFGCAGASTRSGCCLSTYSFLIILFILVELAAGCFIFFDHDWKNVIPVDKTGNFDMIYSFLKENWRIAKWVALGSVVFEALLFTVALIVQSGIQDDYDSDDEYIGARSGVRQPLVNKQAAADPRVPNLDYRPIRNDAWSQRMRDKYGVDTFDPNRFQQATISPAEQRSRCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1I2A6</accession>
    <name>I1I2A6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rubisco LSMT substrate-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100826570</name>
        <name evidence="5" type="ORF">BRADI_3g18940v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234515.1">
        <property type="nucleotide sequence ID" value="XM_010236213.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I2A6"/>
    <dbReference type="STRING" id="15368.I1I2A6"/>
    <dbReference type="EnsemblPlants" id="KQJ95774">
        <property type="protein sequence ID" value="KQJ95774"/>
        <property type="gene ID" value="BRADI_3g18940v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ95775">
        <property type="protein sequence ID" value="KQJ95775"/>
        <property type="gene ID" value="BRADI_3g18940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826570"/>
    <dbReference type="Gramene" id="KQJ95774">
        <property type="protein sequence ID" value="KQJ95774"/>
        <property type="gene ID" value="BRADI_3g18940v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ95775">
        <property type="protein sequence ID" value="KQJ95775"/>
        <property type="gene ID" value="BRADI_3g18940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826570"/>
    <dbReference type="eggNOG" id="ENOG502QRSB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_502882_0_0_1"/>
    <dbReference type="OMA" id="HSLCPHV"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I2A6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1420.10:FF:000006">
        <property type="entry name" value="SET domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000011">
        <property type="entry name" value="Transcription factor, E2F and DP-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF103">
        <property type="entry name" value="N-METHYLTRANSFERASE DOMAIN AND SET DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rubisco LSMT substrate-binding" evidence="4">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ95774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="541" mass="60245" checksum="9D5F63EB5FCD138A" modified="2012-06-13" version="1">MAAEAELASGGDVVVVRLPSISEEDPLHRDKKRILERANLSCLFQVPISSSTADACRLLDKMIHAARVAHMDELELYFAGDDDYGPFSARNELESLNLLLKIMNTLLVTASDGAKGVLQVLEDEIVVRLRSVGLTDNNQMAVQEQNQDTEDSLLKWGEDQGVKSKLQIAFFQGAGRGMVASENIGVGHIALEIPESLIISEELLCQSDMFLALKDLNSITTETMLLLWSMRERHNPSSNFKMFFETLPSNFNTGLNFGIGALAALEGTLLFDELMQARQHLHQQYDELFPMLCTKFPEIFTQDIYTWDNFLWACELWYSNSMMVVLSSGKLTTCLIPVAGLLNHSVYPHILNYGRVDQATKSLKFPLSRPCKAGQQCFLSYGKHSGSHLITFYGFLPREDNPYDVVPLDLDMSVDEEDGTAQSVSSSVTAHMVRGTWLSRSGGPPTYGLPQRLMSHLRTVLNCGHNESIPKADIKENDRMVLETLITIFSPMLEGLGEPDDYNRESASWDLKLALAYKDLKRRIISSIVTSCTSGLALLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1I410</accession>
    <name>I1I410_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100825735</name>
        <name evidence="6" type="ORF">BRADI_3g26850v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573927.1">
        <property type="nucleotide sequence ID" value="XM_003573879.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I410"/>
    <dbReference type="FunCoup" id="I1I410">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I410"/>
    <dbReference type="EnsemblPlants" id="KQJ96730">
        <property type="protein sequence ID" value="KQJ96730"/>
        <property type="gene ID" value="BRADI_3g26850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825735"/>
    <dbReference type="Gramene" id="KQJ96730">
        <property type="protein sequence ID" value="KQJ96730"/>
        <property type="gene ID" value="BRADI_3g26850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825735"/>
    <dbReference type="eggNOG" id="ENOG502QV99">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065258_1_0_1"/>
    <dbReference type="OMA" id="PWITTSI"/>
    <dbReference type="OrthoDB" id="3012298at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000677">
        <property type="entry name" value="Chitinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46476">
        <property type="entry name" value="CHITINASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46476:SF18">
        <property type="entry name" value="GH18 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00551">
        <property type="entry name" value="2SGLOBULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="5">
        <location>
            <begin position="5"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ96730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="288" mass="31477" checksum="50D300D07DAB4084" modified="2012-06-13" version="1">MTNGYVFREYIGAQFKSVQFSDVPVNAGLSFHFILAFAIDYMAATQSRPKPTPANGVFAPFWDAATLSPAAAAATKKAHPNLSIMVGLGGDTVQNTGVNATFAPTSVDTWVANAVSSLSAMINQYGLDGVDVDYEHFAADVDTFVECTGRLLTQLKARFPRMSTSIAPFERPEIQKYYRALWAKYSGVIDYVNFQFYGYGANTNVDYYVGFYNLQLSNYPGSGSKLLASFKTGDVTGLLSPEQGISGAKELQRQGKLPGLFIWSADSSKEAAYKFDYETRAQQIVANH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1I570</accession>
    <name>I1I570_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetraspanin-10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100844389</name>
        <name evidence="7" type="ORF">BRADI_3g30240v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
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    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
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    </evidence>
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    </evidence>
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</entry>
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    </protein>
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        <name evidence="15" type="primary">LOC100823497</name>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
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        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
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        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
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        <text evidence="11">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="function">
        <text evidence="12">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
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            <dbReference type="ChEBI" id="CHEBI:83099"/>
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        </reaction>
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            <name>thiamine diphosphate</name>
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    <comment type="subunit">
        <text evidence="3">Tetramer of 2 alpha and 2 beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="10 12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="13">
        <location>
            <begin position="40"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ99159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="373" mass="39754" checksum="080297E956029A0C" modified="2012-06-13" version="1">MLGAARRSGCVLGQLIQTLRPAAAAAGPAARSYSAAAKEMTVREALNSALDEEMSADPSVFLMGEEVGEYQGAYKITKGLLDKYGPDRVLDTPITEAGFTGIGVGAAYQGLRPVIEFMTFNFSMQAIDHIINSAAKSNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYAHVPGLKVLTPYSAEDARGLLKAAIRDPDPVVFLENELLYGESFPIKDEVLDSSFSVPIGKAKIEREGKDVTITAFSKMVGYALQAAEILSKEGISAEVINLRSIRPLDRAAINASVRKTNRLVTVEEGFPQHGVGAEICMSVVEDSFEYLDAPVERIAGADVPMPYAANLERLAVPQVEDIVRAAKRACYRAGTMAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1I9S0</accession>
    <name>I1I9S0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100843488</name>
        <name evidence="17" type="ORF">BRADI_3g43650v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>oxalate + O2 + 2 H(+) = H2O2 + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:21880"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
            <dbReference type="EC" id="1.2.3.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 15">Secreted</location>
            <location evidence="2 15">Extracellular space</location>
            <location evidence="2 15">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the germin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574983.1">
        <property type="nucleotide sequence ID" value="XM_003574935.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I9S0"/>
    <dbReference type="FunCoup" id="I1I9S0">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99515">
        <property type="protein sequence ID" value="KQJ99515"/>
        <property type="gene ID" value="BRADI_3g43650v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843488"/>
    <dbReference type="Gramene" id="KQJ99515">
        <property type="protein sequence ID" value="KQJ99515"/>
        <property type="gene ID" value="BRADI_3g43650v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843488"/>
    <dbReference type="eggNOG" id="ENOG502QQ4A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015790_0_0_1"/>
    <dbReference type="OMA" id="FACQDPK"/>
    <dbReference type="OrthoDB" id="589868at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050162">
        <property type="term" value="F:oxalate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0052">Apoplast</keyword>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 12 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095152" description="Germin-like protein" evidence="15">
        <location>
            <begin position="21"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="16">
        <location>
            <begin position="59"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQJ99515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="215" mass="22972" checksum="E5086F4748D5DB67" modified="2012-06-13" version="1">MASKFFLLALLALSVSPALASDPSQLQDFCVADRTSPVFVNGFACQDPKIAVSEDFFFSGLHMAGNTSNKQSSAVTAVNVAQIAGLNTLGISLVRVDYAPFGQNPPHIHPRATEILTVLEGSLYVGFMTSNPENKLFAKVLNKGDVFVFPQGLIHFQFNYGTNKAVAIAALSSKNPGVITIGNAVFGSKPSISDDILAKAFQVDKMTVDRIQAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1IAG6</accession>
    <name>I1IAG6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100826564</name>
        <name evidence="18" type="ORF">BRADI_3g45660v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235537.1">
        <property type="nucleotide sequence ID" value="XM_010237235.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IAG6"/>
    <dbReference type="EnsemblPlants" id="KQJ99862">
        <property type="protein sequence ID" value="KQJ99862"/>
        <property type="gene ID" value="BRADI_3g45660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826564"/>
    <dbReference type="Gramene" id="KQJ99862">
        <property type="protein sequence ID" value="KQJ99862"/>
        <property type="gene ID" value="BRADI_3g45660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826564"/>
    <dbReference type="eggNOG" id="KOG0192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_7_29_1"/>
    <dbReference type="OMA" id="ACQNSEY"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000329">
        <property type="entry name" value="PAS domain-containing protein tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000476">
        <property type="entry name" value="PAS domain-containing protein tyrosine kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF110">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0157">Chromophore</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="17">
        <location>
            <begin position="82"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="448"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="349"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQJ99862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="731" mass="81777" checksum="F8527902861204F7" modified="2012-06-13" version="1">MEADELLKKIRLLEEGHAELKRELGKLAPERRGAQLAARRRNSSALPLPSSRRAVAAAAPPQSSRVLRESPGRLSDRHCHWILQSLGQAVHIIAPDGKLLYWNRYAEHMYGYSASEAIGQDAVELICHPADFDAAYVVIQSIFMGKCFRGKFPVKNKSGERFFIVVHNTPLYDDDGSLVGLICLSLDTRALEEIFSPPASAESYQSSAKPYFHANNEPKSGSLDKGSSGSQQPLQTAITSKITTFASKVTRKVRSRIKTGQTCDKQYGSGCEGQYSEHGVRVELTSSEESTPSGDVLHDAFVAEEKSPRKSSKTNSDDSGEGKVGFHKMFTSKAEALLSKKGISWPWKGHETDEGSGKNNMAPTQLHHKQENDESDRRVPVLEPIIIPDCQDTEYAQASTYEVSGSWWTFNNTSMSSMSSPGSTNSSAIDRVDYEGDCLDYEILWEDLVIGEQVGQGSCGTVYHALWYGSDVAVKVFSKQEYSEEMINTFRQEVSLMKKLRHPNIILFMGAAASQQQLCIVTEFLPRGSLFRLLQKNTGKLDPRRRVNMAIDIARGMNYLHNSIPTVVHRDLKSSNLLVDKNWTVKVADFGLSRLKLETFLTTKTGKGTPQWMAPEVLRSEPSNEKSDVYSYGVVLWELITQKVPWDTLNTMQVIGAVGFMDHRLEIPSDADPQWSSMIESCWVSDPQRRPSFRELLERLQVLQKQYTLQAQAQRRVARKGAEKMMSVEDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICV5</accession>
    <name>I1ICV5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Secretory carrier-associated membrane protein</fullName>
            <shortName evidence="8">Secretory carrier membrane protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100837686</name>
        <name evidence="10" type="ORF">BRADI_3g52390v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Probably involved in membrane trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasmic vesicle</location>
            <location evidence="8">Secretory vesicle membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SCAMP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570157.1">
        <property type="nucleotide sequence ID" value="XM_003570109.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICV5"/>
    <dbReference type="STRING" id="15368.I1ICV5"/>
    <dbReference type="EnsemblPlants" id="KQK00877">
        <property type="protein sequence ID" value="KQK00877"/>
        <property type="gene ID" value="BRADI_3g52390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837686"/>
    <dbReference type="Gramene" id="KQK00877">
        <property type="protein sequence ID" value="KQK00877"/>
        <property type="gene ID" value="BRADI_3g52390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837686"/>
    <dbReference type="eggNOG" id="KOG3088">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066546_3_0_1"/>
    <dbReference type="OMA" id="IYFFQTI"/>
    <dbReference type="OrthoDB" id="242866at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ICV5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007273">
        <property type="entry name" value="SCAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687:SF79">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04144">
        <property type="entry name" value="SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK00877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="274" mass="30557" checksum="04E48C46F7D83E67" modified="2012-06-13" version="1">MHRDPNPFDEGADENPFSNGGGRGAAAAGGKSKFQFRPAEPAGFGGGGNSDAAVDIPLDNMNDSNGKAGELSQWQADLKRREADIKRREEALKSAGVPMEDKNWPPFFPIIHHDIANEIPANAQRLQYLAFASWLGIVLCLFWNFIAVTVCWIRGGDSKLFFLATIYGMLGIPLSYLMWYRPLYRAMRTDSAFSFGWFFLCYMLHIGFCIIAAIAPPIVFRGKSLTGILAAIDTFSDHALVGIFYFVGFALFSLETVVSIWVLQRVYMYFRGHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDC6</accession>
    <name>I1IDC6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trehalose 6-phosphate phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100821815</name>
        <name evidence="7" type="ORF">BRADI_3g53790v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570277.1">
        <property type="nucleotide sequence ID" value="XM_003570229.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDC6"/>
    <dbReference type="STRING" id="15368.I1IDC6"/>
    <dbReference type="EnsemblPlants" id="KQK01090">
        <property type="protein sequence ID" value="KQK01090"/>
        <property type="gene ID" value="BRADI_3g53790v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01091">
        <property type="protein sequence ID" value="KQK01091"/>
        <property type="gene ID" value="BRADI_3g53790v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821815"/>
    <dbReference type="Gramene" id="KQK01090">
        <property type="protein sequence ID" value="KQK01090"/>
        <property type="gene ID" value="BRADI_3g53790v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01091">
        <property type="protein sequence ID" value="KQK01091"/>
        <property type="gene ID" value="BRADI_3g53790v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821815"/>
    <dbReference type="eggNOG" id="KOG1050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_3_1_1"/>
    <dbReference type="OMA" id="HSMARIF"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IDC6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000010">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000052">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000054">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000002">
        <property type="entry name" value="Trehalose-6-phosphate synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000079">
        <property type="entry name" value="Trehalose-6-phosphate synthase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF46">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK01090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="851" mass="94763" checksum="7BC40A8F5B0F8AA9" modified="2012-06-13" version="1">MPSLSCHNLLDLANAATDELPPLPPPSPLRLPRVMASGSPSSPASPSPSAPPRRVIVSHRLPLRAAPDAAAPFGFSFTVDASTVAYQLRSGLPPSAPVLHIGTLPAAAAEDFSDELAAYLLANFSCLPVYLPADLHRRFYHGFCKHYMWPLLHYLLPLTPSTLGGLPFDRSLYHSFLSANRAFADRLTEVLSPDDDFVWIQDYHLFALPTFLRKRFPRAKVGFFLHSPFPSSEIFRTIPVRDDLLRALLNADLVGFHTFDYARHFLSACSRLLGLDYQSKRGYIGIEYYGRTVTVKILPVGIDMGQLRSVVSAPETGEVARQVAEAYKGRRLMLGVDDVDLFKGIGLKFLGMEQLLVEHPELRGRAVLVQIANPARSEGRDVQEVQDEARAISARVNERFGSPGYTPIVMINRPVSVHEKAAYYAAAECCVVSAVRDGLNRIPYIYTVCRQESTTQEEAPKRSVIVLSEFVGCSPSLSGAIRVNPWSVESVAEAMNAALMMSEGEQRLRHEKHYKYVSTHDVAYWARSFDQDLQRACKDHFSRRHWGIGFGMSFKVVALGPNFRRLSVEHIVPSFRKTDNRLILLDYDGTVMPESSIDKAPSNEVISVLNRLCEDPKNRVFIVSGRGKDELSKWFAPCEKLGIAAEHGYFTRWSKDSPWETCGLAVDFDWKKTAEPVMRLYTESTDGSYIEHKESALVWHHHEADPDFGSCQAKELLDHLESVLANEPVVVKRGQHIVEVNPQGISKGVVVESLLSSMVRSGKAPDFVLCIGDDRSDEDMFESIVCPTNSSVKLPATSEVFACTVGKKPSMAKYYLDDTVDVIKMLQGLANAPLQQRPWPVQLRVTFEGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IFE7</accession>
    <name>I1IFE7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100825422</name>
        <name evidence="9" type="ORF">BRADI_3g59627v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236135.1">
        <property type="nucleotide sequence ID" value="XM_010237833.3"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IFE7"/>
    <dbReference type="EnsemblPlants" id="KQK01979">
        <property type="protein sequence ID" value="KQK01979"/>
        <property type="gene ID" value="BRADI_3g59627v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825422"/>
    <dbReference type="Gramene" id="KQK01979">
        <property type="protein sequence ID" value="KQK01979"/>
        <property type="gene ID" value="BRADI_3g59627v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001545_0_0_1"/>
    <dbReference type="OMA" id="HAQQNYE"/>
    <dbReference type="OrthoDB" id="343070at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IFE7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057974">
        <property type="entry name" value="NUA/TPR/MLP1-2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012929">
        <property type="entry name" value="Nucleoprot-TPR/MLP1-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057577">
        <property type="entry name" value="Nucleoprot-TPR/MLP1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898:SF2">
        <property type="entry name" value="NUCLEOPROTEIN TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898">
        <property type="entry name" value="NUCLEOPROTEIN TPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25481">
        <property type="entry name" value="Nucleoprot-TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25785">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07926">
        <property type="entry name" value="TPR_MLP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoprotein TPR/MPL1" evidence="7">
        <location>
            <begin position="171"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="NUA/TPR/MLP1-2-like" evidence="8">
        <location>
            <begin position="498"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoprotein TPR/MLP1-2" evidence="6">
        <location>
            <begin position="1045"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1596"/>
            <end position="2069"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="21"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="306"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="453"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="691"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="761"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="842"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="902"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1210"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1265"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1651"/>
            <end position="1663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1696"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1755"/>
            <end position="1769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1809"/>
            <end position="1827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1867"/>
            <end position="1878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1885"/>
            <end position="1898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1933"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2024"/>
            <end position="2033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="2059"/>
            <end position="2069"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK01979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="2069" mass="233319" checksum="3F02481C0C52C151" modified="2012-06-13" version="1">MPLFMSDEELRLLGGDVAAVAERADAAIREYRNQVDTVRAEADAAAIAAEQTCALLEQRFATLSADADRYQAEATELAAASERRAADLASSQAEIHQLRIQAIAKDGEVERLKVEISELHKSKCQSLELIQQRDAEIREKDGVIQSYHDKIVNQADSSAGKEARIQEVEAKLTHCQATCNRIAQEKELLQKHNLWLDEELKAKVKNLADLRKANMEEESRMSAKIAELEREVSESSSSLRRSKEQSSELEQRVTYMEKELFSAKDAAAANEQRLGAELSTVMKLAELHKESSEEWSKKAGELEGVIKALETHLTQVEDDYKEKLEKETLAKSDLEKEVANLKQKIQKCESDLENSRKSGELSLIPLTSMAADPAALADTQLREMPLSDALNQNDLMIVPSVPTGVSGTALAASLLRDGWSLAKIYEKYQEATDALRHERWGRRHAEAVLERVLHEIEEKAELILDERAEHERMIEAYALMDHKLQQALLEHDNFENTIRNLKSELKRRERDHTIANKEIDDLQKQVAVLLKECQDIQLRCGSSLPNVGHGALSTSINNGLSIVENNSHEHMTFNDINGLVQQNVQLRNKVHLLSTDLDKKDMELRESFQIELKRITDGATSRVEKVMKKSEEQAIMIESLHRSVAMYRKLCEEQQKTRSNVEHISNNLQDDGRKDLMVLFEGSQDVSKKAYEQVSERSKRLDEELTKLRTELVSLRSERDKSVLEAKFAQDRLNGYMTENDHQRKEANSVSLRNAELMHLIVDYEKRLRESSDSMQALEENSRKLSMEVSILKHEKEIVVKSERRSLDEVHDLTERVHRLQGTIDTIHTTEEVQENARSMERRNQQEYIKRLERDWAELKKEVQEQRDHVRVLTLDKQNAFDGCMKQVEDMRKELQSSWKACTDAESRAAVAEVKCADLEAKLKSKMIIFRDGGRDNSSATEENDELFRQKEELEKYKDEAHANKSYMLQYKEIANSNEAALKQMETAYQDYKRESEVAKRSLEDEIAKLRSKLSEMDKSYVMKCEEAANAIEAKEKDITALMSDISILRNELSQKVARIENLEIESASSKSALDEQCKRWRSAQDNYERQVILQSETIQELTNTSKQLSSLQQEVTILRQTADAQKAENDALRTFGEQEKVQLLKEKDEALRKYTELNDQNRILHNRLEALHIRLAEQERGIAGLSSQRTDSHGEDDLHGVVSYLRRSKEIAETEILLLKQEKSRLQIELESALKSAKEAHEVLRSQADSARSSMFKDEEFKSLQLQVREINLLRESNIQLREENRHNFEECQKFREEAHKAIREAERLHDILQEKQVASEIGAKELELQKSEIVNLNQRISELVENSRGVDLSTYETMKDELQNIKSTLRENSIELERAKTLLSEREVVIRNLEEKLAGCQSELAVKEKKLNEVEASLKSEMERLKRINFAIKKKLEISTKEKENATKENNSLLKQIEDLKSSQKTTSETANDQVVKEKESRIQTLEKILERERDDNRKEKSSRKKAESVFLGALQNLSKEKKQIEDSIEKHRQAVKEAIEHYSGQSSQIPSGSAVEEQFRSYFLAAKRIEESSSPFKDGSVNQTSLIDTPTVDAAATGRPVVTPANPAQVKGVEERAVSTSSKPSTERRLRRPLVRPTLERVEEPQADTDTPITEGSTVAQEKGSLSLGRGSTVNQEKGGPSLERGSTIVQEKGGRLLERGLGQDKVGLSLERETSGGVSVPSSRKRLISLSQGRDDAPQGEASDSNPPSKKPKEEFFHGTSEQKNDQSASEDVTAQAPVDPSMDNQDAQQPADEMDTDQASMPIEEVEDTRDGDVGVNDDMEERTDASTGIKDQYTEVHIDSGATAVEDMPVKSEAVEESFDEDQKLDDAKEEGQITTATDVEEEREEGELPEESEQHSDSSPPDIGEQAADSFRVASPGGLTEKSDTDMAEEVGEGETAEHGTVEADQSPLAQSGGADASPGRNTDVSPAREPSPSNPPHDGASSEQQIPATAIDTGETQNPTLNFERARQTRQARFGRTTSPQSATNRGRPRTPIVFPKDTGSGSQKEAARGSRGRRGRGGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1IFL7</accession>
    <name>I1IFL7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106866427</name>
        <name evidence="5" type="ORF">BRADI_3g60230v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in the defense reaction of plants against pathogens.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK02078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014756083.2">
        <property type="nucleotide sequence ID" value="XM_014900597.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317559.1">
        <property type="nucleotide sequence ID" value="XM_024461791.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317560.1">
        <property type="nucleotide sequence ID" value="XM_024461792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IFL7"/>
    <dbReference type="EnsemblPlants" id="KQK02078">
        <property type="protein sequence ID" value="KQK02078"/>
        <property type="gene ID" value="BRADI_3g60230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106866427"/>
    <dbReference type="Gramene" id="KQK02078">
        <property type="protein sequence ID" value="KQK02078"/>
        <property type="gene ID" value="BRADI_3g60230v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035730_8_3_1"/>
    <dbReference type="OMA" id="AKWWASS"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05381">
        <property type="entry name" value="CAP_PR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000004">
        <property type="entry name" value="CAP, cysteine-rich secretory protein, antigen 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002413">
        <property type="entry name" value="V5_allergen-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00838">
        <property type="entry name" value="V5ALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095280" description="SCP domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="4">
        <location>
            <begin position="60"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK02078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="205" mass="22270" checksum="E6CAA3555294E306" modified="2012-06-13" version="1">MEPPLGALLVLCSLLLLAYPSSSAAHGHGHYHEHYDNATVYNVSVAAAAAAGTQGLGAWADALEFLYYHNAVRMARWEPPLAWSPRLESYARWWAAQRRADGCALRHSFPDGQFALGENIFWGSGAAASWRPGDAVKEWAAEGVDYSYAANACAPGRECAHYTQIVWRRTALLGCARVVCGDDGGGVFMTCNYYPPGNVVGERPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1IGR8</accession>
    <name>I1IGR8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100833839</name>
        <name evidence="13" type="ORF">BRADI_4g02600v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the Aux/IAA family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579189.1">
        <property type="nucleotide sequence ID" value="XM_003579141.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IGR8"/>
    <dbReference type="FunCoup" id="I1IGR8">
        <property type="interactions" value="945"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IGR8"/>
    <dbReference type="EnsemblPlants" id="KQJ85961">
        <property type="protein sequence ID" value="KQJ85961"/>
        <property type="gene ID" value="BRADI_4g02600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833839"/>
    <dbReference type="Gramene" id="KQJ85961">
        <property type="protein sequence ID" value="KQJ85961"/>
        <property type="gene ID" value="BRADI_4g02600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833839"/>
    <dbReference type="eggNOG" id="ENOG502QPNB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049393_1_5_1"/>
    <dbReference type="OMA" id="IGTCGSQ"/>
    <dbReference type="OrthoDB" id="642974at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IGR8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000078">
        <property type="entry name" value="Auxin-responsive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF103">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="132"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ85961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="249" mass="27005" checksum="64116228FB0AFD4A" modified="2012-06-13" version="1">MAAADLGFEATELRLGLPGGDGGEQARKRGFAETIDLKLKLEPAGEEEPAAAEEEVEVKAEVPESEEEKAADGGKMKRSPSQSSVVTAAAAAMADPAEKPRAPKAQVVGWPPVRSFRKNILQAEKSSSSSPAAFVKVSMDGAPYLRKVDLNMYKTYQDLSMALHKMFSSFTIGNCGSQGMNGMNESKLMDLLNGSEYVPTYEDKDGDWMLVGDVPWEMFVESCKRLRIMKGSEAIGLAPRAMEKCKNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJN8</accession>
    <name>I1IJN8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC112272454</name>
        <name evidence="1" type="ORF">BRADI_4g11157v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024319022.1">
        <property type="nucleotide sequence ID" value="XM_024463254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJN8"/>
    <dbReference type="STRING" id="15368.I1IJN8"/>
    <dbReference type="EnsemblPlants" id="KQJ87448">
        <property type="protein sequence ID" value="KQJ87448"/>
        <property type="gene ID" value="BRADI_4g11157v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112272454"/>
    <dbReference type="Gramene" id="KQJ87448">
        <property type="protein sequence ID" value="KQJ87448"/>
        <property type="gene ID" value="BRADI_4g11157v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUY1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017798_6_0_1"/>
    <dbReference type="OMA" id="RPQATEI"/>
    <dbReference type="OrthoDB" id="624323at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004320">
        <property type="entry name" value="BPS1_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33070">
        <property type="entry name" value="OS06G0725500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33070:SF124">
        <property type="entry name" value="SYNTAXIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03087">
        <property type="entry name" value="BPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ87448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="235" mass="26541" checksum="7206FA195657273E" modified="2012-06-13" version="1">MAFHQRSISLPSRPHISETEVEQELQSLETSISSSITIGTMCDGLRRLVNIYNGVEEIICLPSNQVFSIQKRKMLDAEMEGSLDLLDLCSTMQEIFAEMKAIIQDLQVALRKGNDTAVQAKIQSYACLAKKVKKHFKKTTKKAMSDKECRMVMLLTTAREVSISLLECTFHLLFKQIEMPKQSLVYKAFHKKKAVVCEDQLQELESSVGDLEDGAAHLFRKLLQSRVSLLNILSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IK75</accession>
    <name>I1IK75_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100838113</name>
        <name evidence="3" type="ORF">BRADI_4g13202v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575829.1">
        <property type="nucleotide sequence ID" value="XM_003575781.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IK75"/>
    <dbReference type="STRING" id="15368.I1IK75"/>
    <dbReference type="EnsemblPlants" id="PNT63223">
        <property type="protein sequence ID" value="PNT63223"/>
        <property type="gene ID" value="BRADI_4g13202v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838113"/>
    <dbReference type="Gramene" id="PNT63223">
        <property type="protein sequence ID" value="PNT63223"/>
        <property type="gene ID" value="BRADI_4g13202v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838113"/>
    <dbReference type="eggNOG" id="KOG0001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_0_0_1"/>
    <dbReference type="OMA" id="SHDTINV"/>
    <dbReference type="OrthoDB" id="680853at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019941">
        <property type="term" value="P:modification-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000562">
        <property type="entry name" value="Polyubiquitin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000211">
        <property type="entry name" value="Polyubiquitin 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="66"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="147"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="224" mass="25444" checksum="750A0AC3EF0893F8" modified="2012-06-13" version="1">MYIFVKNPTSRTICLKVQSSDTLCTVKAKIQEQHFLAFDGKKLEDNLTLADYDIQHGSMLDLQEKMQIYVKETLADRTIILEVDSLDTIGNVKAKMEDKDGFLKGQQCLIFGSKQLEDERTLADHNVLKDSTLLLVLHPSLPRGTTMHIYVQKVEGKGKPITLEVESHDTINVVKMKISEKDGTRPIQCRLMFDCTVLKDYRTLADYGIERGDTLDFFMCLCGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IN88</accession>
    <name>I1IN88_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst subunit Exo70 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100826064</name>
        <name evidence="6" type="ORF">BRADI_4g24680v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the EXO70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758405.1">
        <property type="nucleotide sequence ID" value="XM_014902919.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IN88"/>
    <dbReference type="FunCoup" id="I1IN88">
        <property type="interactions" value="1866"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IN88"/>
    <dbReference type="EnsemblPlants" id="PNT64113">
        <property type="protein sequence ID" value="PNT64113"/>
        <property type="gene ID" value="BRADI_4g24680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826064"/>
    <dbReference type="Gramene" id="PNT64113">
        <property type="protein sequence ID" value="PNT64113"/>
        <property type="gene ID" value="BRADI_4g24680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826064"/>
    <dbReference type="eggNOG" id="KOG2344">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010236_2_1_1"/>
    <dbReference type="OMA" id="WAPNEAG"/>
    <dbReference type="OrthoDB" id="1922221at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF127">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT EXO70C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="5">
        <location>
            <begin position="364"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="53"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="232"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="538"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="553"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT64113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="768" mass="83074" checksum="B84CAB43B9CBAC5A" modified="2012-06-13" version="1">MERIPIPMAMLPQKSSSFSQATVRQHQQQGSDHEKLGRNLSLGAIKLSEHVDRLKKESAAAAEEASSAAAAGGEEAEPPPYEEPDLAALSVEIDAFLASRAEGDSAAAISEVTLDRFASAVEMEIAATVEGDDDRWASAAAEDGGGGGEQKNPPRVLLDAIRRISTLAAALTTNNAAPAAAAAETDAAATAGGGNKKGYTIGVHRVTGVLHRAMAFLEDELYALLEDIPSNSNPGSAKAMRRPPSFSAAAHGADSDRCVLPLPSSATATTTTTPETPAGAGGDNSSASNSNQPQPLFPPETVDRLRAMADAMAHAGYSTECEQVFLISRRNALDSALQALGYEKASIDDVVKMSWESLEAEIGAWIKAFRHVINVGLSAEHDLCVRVFPPSSSNGNGNGNVGKEIFADLARCALLQMLNFTEAVAMAKRAAEKLFKVLDMYEAIRDSAPVVDAFLDMYTPNAGTGHEALSDLQSELASVQSRLGESAAAIFCDLESSIRADAGKQPVPGGAVHPLTRYLMNYLKYACEYKNTLEQVFRQHHHRPDSDDPPPSSTDNNSSSNNNNNNANTNENNPFAAQLMEVMELLHGNLEAKSRLYKDPALSSIFLMNNGRYMLQKIRGSPEINAVVGEAWARKRSTDLRQYHKNYQRETWNRVLNMLRDDGSITVKGHVQKPVLKERFKQFNAAMDEIHRNQGSWVVSDDQLQSELRVSIAAVVVPAYRSFLGRFAQSFSAGRQTEKYIKLSADDLENIIDELFDGNTASMPRRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1IR78</accession>
    <name>I1IR78_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100828929</name>
        <name evidence="2" type="ORF">BRADI_4g33590v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576673.1">
        <property type="nucleotide sequence ID" value="XM_003576625.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IR78"/>
    <dbReference type="EnsemblPlants" id="KQJ90734">
        <property type="protein sequence ID" value="KQJ90734"/>
        <property type="gene ID" value="BRADI_4g33590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828929"/>
    <dbReference type="Gramene" id="KQJ90734">
        <property type="protein sequence ID" value="KQJ90734"/>
        <property type="gene ID" value="BRADI_4g33590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828929"/>
    <dbReference type="eggNOG" id="KOG3336">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067902_5_0_1"/>
    <dbReference type="OMA" id="PKQQTFE"/>
    <dbReference type="OrthoDB" id="407630at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990050">
        <property type="term" value="F:phosphatidic acid transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ90734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="188" mass="20893" checksum="668D500CF7C1DE60" modified="2012-06-13" version="1">MVVSYTQEHVYRHPWHRVTAAAWRKFTDPAAREAPLSHILDVQTLSRRLDPRAGRLHAVRAIAGRAPAPLPFLLRPLAAGVDAVLCVERTTVDCPARAMLVISRNANLRRLVDVEERCRYGPHPERPEEWTLVTQETTIRCAPLAAVSAVVAGLVERRCADSFVQNAAKGQEVVERICQGLALADHED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IUK4</accession>
    <name>I1IUK4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_4g43190v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IUK4"/>
    <dbReference type="MEROPS" id="A01.086"/>
    <dbReference type="EnsemblPlants" id="PNT65487">
        <property type="protein sequence ID" value="PNT65487"/>
        <property type="gene ID" value="BRADI_4g43190v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65487">
        <property type="protein sequence ID" value="PNT65487"/>
        <property type="gene ID" value="BRADI_4g43190v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005738_5_2_1"/>
    <dbReference type="InParanoid" id="I1IUK4"/>
    <dbReference type="OrthoDB" id="632849at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000077">
        <property type="entry name" value="Aspartic proteinase nepenthesin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF57">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT65487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="332" mass="35743" checksum="5843424E6EF6C85B" modified="2012-06-13" version="1">MDISLGTPPVANLVTVDTGSTLSWVVCQRCQISCHTTAPEAGSVFDPDKSTTYELVGCSSRDCADVQRSLVAPFGCIEETDTCLYSLRYGSGPSGQYSAGRLGTDKLTLASSSSIIDGFIFGCSGDDSFKGYESGVIGFGGANFSFFNQVARQTNYRAFSYCFPGDHTAEGFLSIGAYPKDELVYTNLIPHFGDRSVYSLQQIDMMVDGNRLQVDQSEYTKRMMVVDSGTVDTFLLGPVFDAFSKAMASAMQAKGFLSDTVGTETCFRPNGGDSVDSGDLPTVEMRFIGTTLKLPPENVFHDLLPSHDKICLAFKPDVAGVRNVQILGNKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVE3</accession>
    <name>I1IVE3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ent-kaurenoic acid monooxygenase</fullName>
            <ecNumber evidence="14">1.14.14.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100829065</name>
        <name evidence="22" type="ORF">BRADI_5g00467v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ent-7alpha-hydroxykaur-16-en-19-oate + reduced [NADPH--hemoprotein reductase] + O2 = gibberellin A12 aldehyde + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22904"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57298"/>
            <dbReference type="ChEBI" id="CHEBI:57432"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:22905"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ent-kaur-16-en-19-oate + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = gibberellin A12 + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:33219"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57297"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58627"/>
            <dbReference type="EC" id="1.14.14.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:33220"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ent-kaur-16-en-19-oate + reduced [NADPH--hemoprotein reductase] + O2 = ent-7alpha-hydroxykaur-16-en-19-oate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19241"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57297"/>
            <dbReference type="ChEBI" id="CHEBI:57298"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19242"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>gibberellin A12 aldehyde + reduced [NADPH--hemoprotein reductase] + O2 = gibberellin A12 + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22700"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57432"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58627"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:22701"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Plant hormone biosynthesis; gibberellin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.107" evidence="14"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580920.1">
        <property type="nucleotide sequence ID" value="XM_003580872.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVE3"/>
    <dbReference type="STRING" id="15368.I1IVE3"/>
    <dbReference type="EnsemblPlants" id="KQJ81398">
        <property type="protein sequence ID" value="KQJ81398"/>
        <property type="gene ID" value="BRADI_5g00467v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829065"/>
    <dbReference type="Gramene" id="KQJ81398">
        <property type="protein sequence ID" value="KQJ81398"/>
        <property type="gene ID" value="BRADI_5g00467v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829065"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_15_5_1"/>
    <dbReference type="OMA" id="TMWATIL"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051777">
        <property type="term" value="F:ent-kaurenoic acid monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009686">
        <property type="term" value="P:gibberellin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048868">
        <property type="term" value="P:pollen tube development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000052">
        <property type="entry name" value="Ent-kaurenoic acid oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF356">
        <property type="entry name" value="ENT-KAURENOIC ACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 19 20" id="KW-0349">Heme</keyword>
    <keyword evidence="10 19 20" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 20" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095571" description="ent-kaurenoic acid monooxygenase" evidence="21">
        <location>
            <begin position="20"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KQJ81398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="493" mass="56049" checksum="B4B655C6833B0BD3" modified="2012-06-13" version="1">MWWWAAVAVGLAVVVVVDGVVRKAHAWYWVGSLGAERRARLPPGDMGWPLVGGMWAFLRAFKSGKPDAFIDSLVGRYGRTGLYRSFMYSSPSVLVTTPEACKKVLMDDVAFVNGWPEATLNLIGTRSFTSPAMPRDEHRRLRKLTAAPVNGSTALAAYLGFIDHNVTSTLRRWSSSSEPVEFLTELRRMTFLIIVRIFMSRAAEDEGTMAALEQSYSELNHGMRAMAINLPGFAYHKALKARRKLVAVLQGVLDDRRRCPRQASVDMDMMDRLIEVEDEGGRRLEDEEIIDMLVLYLNAGHESSAHITMWATVFMQENSHILSKAKAEQEEIMKNIPLTQKGLTLRDYKKMEYLSQVIDETLRFVNISFVTFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQLYSEPNKFNPSRWEGPPPKVGTFLPFGLGPKLCPGNDLAKLEISVFLHHFLLGYKLTRENPSCRIRYLPHPRPVDNCLAKVTRVSSNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVH3</accession>
    <name>I1IVH3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Ethylene receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">LOC100833656</name>
        <name evidence="30" type="ORF">BRADI_5g00700v3</name>
    </gene>
    <organism evidence="30">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">Bd21</strain>
            <strain evidence="31">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="30">Bd21</strain>
        </source>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="31">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Ethylene receptor related to bacterial two-component regulators. Acts as a negative regulator of ethylene signaling. May delay the transition from the vegetative stage to the floral stage by up-regulating GI (GIGANTEA) and RCN1 and cause starch accumulation in stems by down-regulating the alpha-amylase AMY3D.</text>
    </comment>
    <comment type="function">
        <text evidence="20">May act early in the ethylene signal transduction pathway, possibly as an ethylene receptor, or as a regulator of the pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="22">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="22">Binds 1 copper ion per dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the ethylene receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580931.1">
        <property type="nucleotide sequence ID" value="XM_003580883.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751528.1">
        <property type="nucleotide sequence ID" value="XM_014896042.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVH3"/>
    <dbReference type="STRING" id="15368.I1IVH3"/>
    <dbReference type="EnsemblPlants" id="KQJ81436">
        <property type="protein sequence ID" value="KQJ81436"/>
        <property type="gene ID" value="BRADI_5g00700v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ81437">
        <property type="protein sequence ID" value="KQJ81437"/>
        <property type="gene ID" value="BRADI_5g00700v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT60491">
        <property type="protein sequence ID" value="PNT60491"/>
        <property type="gene ID" value="BRADI_5g00700v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT60492">
        <property type="protein sequence ID" value="PNT60492"/>
        <property type="gene ID" value="BRADI_5g00700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833656"/>
    <dbReference type="Gramene" id="KQJ81436">
        <property type="protein sequence ID" value="KQJ81436"/>
        <property type="gene ID" value="BRADI_5g00700v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81437">
        <property type="protein sequence ID" value="KQJ81437"/>
        <property type="gene ID" value="BRADI_5g00700v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60491">
        <property type="protein sequence ID" value="PNT60491"/>
        <property type="gene ID" value="BRADI_5g00700v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60492">
        <property type="protein sequence ID" value="PNT60492"/>
        <property type="gene ID" value="BRADI_5g00700v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833656"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_48_1"/>
    <dbReference type="OMA" id="FNCCDED"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051740">
        <property type="term" value="F:ethylene binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038199">
        <property type="term" value="F:ethylene receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000904">
        <property type="term" value="P:regulation of starch metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd16938">
        <property type="entry name" value="HATPase_ETR2_ERS2-EIN4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19933">
        <property type="entry name" value="REC_ETR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000094">
        <property type="entry name" value="Ethylene receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000240">
        <property type="entry name" value="Ethylene receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000087">
        <property type="entry name" value="Ethylene receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014525">
        <property type="entry name" value="ETR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058544">
        <property type="entry name" value="ETR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24423:SF633">
        <property type="entry name" value="ETHYLENE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24423">
        <property type="entry name" value="TWO-COMPONENT SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25487">
        <property type="entry name" value="ETR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026389">
        <property type="entry name" value="Ethyln_sen_HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 20 22" id="KW-0186">Copper</keyword>
    <keyword evidence="17 23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 20" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 20" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="10 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 20 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 20 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 25" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="27" id="KW-0732">Signal</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15 20" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095551" description="Ethylene receptor" evidence="27">
        <location>
            <begin position="25"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="28">
        <location>
            <begin position="388"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="29">
        <location>
            <begin position="647"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="570"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="25">
        <location>
            <position position="698"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="23">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)" evidence="24">
        <location>
            <position position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00056438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR026389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR026389-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR026389-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR026389-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR026389-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="KQJ81437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="769" mass="85402" checksum="FF42BDDB790A5A0C" modified="2012-06-13" version="1">MAGAARPRIPFLWVLMFLFSLLLPLPSVAPTAADFSHCGGCDDGDGALWSTESILQCQKVSDFLIAAAYFSIPLELLYFTTCSDLFPLKWIFLQFGAFIVLCGLTHLIAVFTYEPHSFHVVLALTVAKFLTALVSFATAITLLTLIPQLLRVKVRENFLRIKARELDQEVGKMKRQEEASWHVRMLTQEIRKSLDRHTILYTTMVELSKTLELQNCAVWMPNESRTEMILTHHLREREITESHSGSIPIGDPDVVQIKATRGAIVLGEDSALGIASRCNPQAGAAVAAIRMPMLRVSNFKGGTPEVMQTSYAILVLVLPEDGSLGWGEQELEIVEVIADQVAVALSHAAVLEESQLMREKLAQQHRDLLRAKHEAMMATEARNSFQSAMYDGMRRPMHSILGLVSMMQQESMNPEQRLVMDAIVKTSSVASTLMNDVMQTSTMNREHLSLVRRPFSLHSLIKEAVSVVRCLSGAKGVDFEFQVDNSLPERVVGDEKRVFHIVLHMVGTLIHQCSAGCLSLHVNSYNDMEERHNQDWMLQRANLSAGYVCVKFMIRIRKSEDNLLGSSSSKISQEPNASSSEMGLSFNMCKKIVQMMNGNIWSISDSKGLRETVMLALQFQVQHVTPVSGASSDLYRSSPIPNFNGLHVLLVDGDDTNRAVTHKLLEKLGCRVFSVSSGIQCMSSFAGGESSFQLVIVDLTMHTMDGFEVALAIRKFRHNSWPPLIVALAASADDSVRDQCQRSGINGLLQKPVTLAALGDELYRVLQHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVJ5</accession>
    <name>I1IVJ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC100838555</name>
        <name evidence="23" type="ORF">BRADI_5g00925v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">Bd21</strain>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580943.1">
        <property type="nucleotide sequence ID" value="XM_003580895.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239605.1">
        <property type="nucleotide sequence ID" value="XM_010241303.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311419.1">
        <property type="nucleotide sequence ID" value="XM_024455651.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311420.1">
        <property type="nucleotide sequence ID" value="XM_024455652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVJ5"/>
    <dbReference type="STRING" id="15368.I1IVJ5"/>
    <dbReference type="EnsemblPlants" id="KQJ81469">
        <property type="protein sequence ID" value="KQJ81469"/>
        <property type="gene ID" value="BRADI_5g00925v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838555"/>
    <dbReference type="Gramene" id="KQJ81469">
        <property type="protein sequence ID" value="KQJ81469"/>
        <property type="gene ID" value="BRADI_5g00925v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838555"/>
    <dbReference type="eggNOG" id="ENOG502QTX3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_62_6_1"/>
    <dbReference type="OMA" id="WDPSGRH"/>
    <dbReference type="OrthoDB" id="630636at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000238">
        <property type="entry name" value="Os04g0141400 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000883">
        <property type="entry name" value="OSJNBb0096E05.1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019825">
        <property type="entry name" value="Lectin_legB_Mn/Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00307">
        <property type="entry name" value="LECTIN_LEGUME_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095575" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="23"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="365"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="320"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="694"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="694"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KQJ81469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="713" mass="77023" checksum="D0ADF33ED0F19ACC" modified="2012-06-13" version="1">MAVLKLVAMAVLICVHVHHSAAVSFSYSSFSSVDFREEDDARITAAGRIELLGDESGGRARGRVVYKKPVQLWDGVTGEATNFTATFNVNITSLPGRSSSAVGHGMAFFLAPYMPDLPQESYDGCLGLFDESLIQPAQQNGTTAPIPSANATGGARFVAVELDTHRDAWDPSGRHVGVDVNSVDSRGNYVILPDASLVDAGVMSVTVSYDSAMTSLDVALVVGATGATYRLAAVVDLRSLLPEQVAVGFSAATGDMFASDHAVLSCSFHSTLATRNNNTSTSSSSKKATVLLHVGVTAAAVLVLLLGLTVGLLLRRASRRPPDNEKKDMLAGDEDDDSLDDDEFGSSTGPRSIPYAQLAASTEDFAEERKLGQGGSGSVYRGHMEELDDGRRDVAIKVFLRGASLEGRKEYRTEVTVISRLRHRNLVQLIGWCHGRRRLLLVYELVHHGSLDRYLYGSKQETLTWQLRYQIILGLASAVLYLHQEWDQCVVHGDIKPSNIMLDESFNTKLGDFGLSRLIDHGMSLQTMTAMAGTPGYLDPECVISGKASTESDVYSFGITLLEIACGRRPMAPPSADGKQVFRLLEWVWDLYGRGAALDAADQRLGGTFDRWEMERVVAIGLWSAHPDPKMRPAIRQAAEALQSRKFRMPVLPPRMLVAVYLQPFGASTMEYGYGGDSNTTVGSSVTMQCSLTSQTTATQSSNSSVPAGDSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1IYI6</accession>
    <name>I1IYI6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100823837</name>
        <name evidence="9" type="ORF">BRADI_5g12500v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579910.1">
        <property type="nucleotide sequence ID" value="XM_003579862.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IYI6"/>
    <dbReference type="FunCoup" id="I1IYI6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ82995">
        <property type="protein sequence ID" value="KQJ82995"/>
        <property type="gene ID" value="BRADI_5g12500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823837"/>
    <dbReference type="Gramene" id="KQJ82995">
        <property type="protein sequence ID" value="KQJ82995"/>
        <property type="gene ID" value="BRADI_5g12500v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823837"/>
    <dbReference type="eggNOG" id="KOG1303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031160_0_0_1"/>
    <dbReference type="OMA" id="PYINNCY"/>
    <dbReference type="OrthoDB" id="40134at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IYI6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="22"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ82995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="435" mass="47324" checksum="3E0BFD899FDB3E49" modified="2012-06-13" version="1">MASPSSVLPKVVDGENEATGRRAKWWYVTFHNVTAMVGAGVLSLPYAMAHLGWGPGIVALVVSWGMTLYTLRLLILMHECVPGVRFDRYRDLGVHALGPRLGLWVVVPQQLIVQVGCDVVYMVTGGNCLQKFFESVCPSCSPRLHGSYWICIFGSSQFLLSQLRDLNSITAISLAAAAMSLSYSTISWAACLARGPVAGVSYAYNKAGTASDGVFRVCSALGQVAFAFAGHGVVLEVQATIPSSATKPSRVPMWKGTVAAYLVTAACYFPVAFVGYWTFGRDVSDNVLVALERPPWLVAAANLMVVVHVVGSYQVYAMPVFESIETILVNKFRVPRGVLLRLVARSTYVAFTLFVAVTFPFFGDLLGFFGGFGFTPTSFFLPCILWLRIKKPPRFSASWFANWGCIVVGVMLMLVSTIGGLRSIIQDASTFQFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZK8</accession>
    <name>I1IZK8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g15470v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZK8"/>
    <dbReference type="FunCoup" id="I1IZK8">
        <property type="interactions" value="1525"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IZK8"/>
    <dbReference type="EnsemblPlants" id="KQJ83532">
        <property type="protein sequence ID" value="KQJ83532"/>
        <property type="gene ID" value="BRADI_5g15470v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83532">
        <property type="protein sequence ID" value="KQJ83532"/>
        <property type="gene ID" value="BRADI_5g15470v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPPJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040235_1_0_1"/>
    <dbReference type="InParanoid" id="I1IZK8"/>
    <dbReference type="OMA" id="CKGIPNT"/>
    <dbReference type="OrthoDB" id="1925835at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007142">
        <property type="term" value="P:male meiosis II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039300">
        <property type="entry name" value="JASON"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33318">
        <property type="entry name" value="ASPARTYL/GLUTAMYL-TRNA(ASN/GLN) AMIDOTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33318:SF4">
        <property type="entry name" value="OS04G0511700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ83532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="521" mass="57351" checksum="E73D810939E42201" modified="2012-06-13" version="1">MGCFFACFGGGDEDRRRRKSRRRSPSSHPPRPDHHVARVGKASLEEDEVVKEASPPLPDVRASAPPPPLVVEASDELVTGPNPGTEQRELREQKTLTPPSLTVHVAEEVVSGASPGGLSQLNDHKELPARSLPQEAIVTPPLFTMKCSPVATTVVSTPDMELREVSEEENRSSGKKKVSFNMNVTSYENTSLPDQEEERSESIKWMKDEDEKHMQKTVLLPENHRYGNCTDSDDDIGDEYCEDDNCGDYSDTEEDFVECKVDLVDEEEMSTDENKEESHESLFSLPMSKDRQNDQEVTSPIPKSSEGPAQEESPLIQGNSHRDRSKYVHPVLNPVQNLSQWKEVKAQVGPVNKLYKENVNSGPDAGASPSSKVANQTKMSPSNASKGDVSVDASLSTWLVSSGNSTVDKAQSKSPRSVSSICREERPVLGALTVDGLKKSSTTSSPRRSPSHNRQEVPILGTVGSYWSCTEPDNECCSSRSDSGTNGIPNTTSKYREDRRVNWHSTPFNVRLDKALKKSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1J038</accession>
    <name>I1J038_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100845555</name>
        <name evidence="10" type="ORF">BRADI_5g16910v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YABBY family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240181.1">
        <property type="nucleotide sequence ID" value="XM_010241879.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J038"/>
    <dbReference type="FunCoup" id="I1J038">
        <property type="interactions" value="425"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J038"/>
    <dbReference type="EnsemblPlants" id="KQJ83797">
        <property type="protein sequence ID" value="KQJ83797"/>
        <property type="gene ID" value="BRADI_5g16910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845555"/>
    <dbReference type="Gramene" id="KQJ83797">
        <property type="protein sequence ID" value="KQJ83797"/>
        <property type="gene ID" value="BRADI_5g16910v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845555"/>
    <dbReference type="eggNOG" id="ENOG502QQ88">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071156_1_0_1"/>
    <dbReference type="OMA" id="AGECMLF"/>
    <dbReference type="OrthoDB" id="667577at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010158">
        <property type="term" value="P:abaxial cell fate specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010154">
        <property type="term" value="P:fruit development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009944">
        <property type="term" value="P:polarity specification of adaxial/abaxial axis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000024">
        <property type="term" value="P:regulation of leaf development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902183">
        <property type="term" value="P:regulation of shoot apical meristem development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000012">
        <property type="entry name" value="axial regulator YABBY 5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006780">
        <property type="entry name" value="YABBY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056775">
        <property type="entry name" value="YABBY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056776">
        <property type="entry name" value="YABBY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31675:SF50">
        <property type="entry name" value="PROTEIN YABBY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31675">
        <property type="entry name" value="PROTEIN YABBY 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04690">
        <property type="entry name" value="YABBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24868">
        <property type="entry name" value="YABBY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="YABBY N-terminal" evidence="9">
        <location>
            <begin position="29"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="YABBY protein C-terminal" evidence="8">
        <location>
            <begin position="153"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="114"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ83797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="265" mass="28587" checksum="9232D206F9A3958D" modified="2012-06-13" version="1">MMSPAESSETSFFPDHIVEQQEQPPFVEQEQLCYVHCNFCDTILAVGVPCSSLFKTVAVRCGHCANLLSVNLRSLLLPAAAPNQLPFGQSLISPTSPASPHGLLDEMSSFQAPSSLLTEQSSPNVSSITSSNNSSAINTPATMSMPPEKAAQREPQTRKNASSGSTKHPEKRQRVPSAYNRFIKDEIQRIKANNPDITHREAFSAAAKNWAHFPHIHFGLMPDQALRKTSIQSQDAAGDCMLFKDSLYAAAAAAAAASSMGVTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1J080</accession>
    <name>I1J080_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100828152</name>
        <name evidence="9" type="ORF">BRADI_5g17300v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580258.1">
        <property type="nucleotide sequence ID" value="XM_003580210.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J080"/>
    <dbReference type="STRING" id="15368.I1J080"/>
    <dbReference type="EnsemblPlants" id="KQJ83874">
        <property type="protein sequence ID" value="KQJ83874"/>
        <property type="gene ID" value="BRADI_5g17300v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828152"/>
    <dbReference type="Gramene" id="KQJ83874">
        <property type="protein sequence ID" value="KQJ83874"/>
        <property type="gene ID" value="BRADI_5g17300v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828152"/>
    <dbReference type="eggNOG" id="ENOG502QQWK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018354_6_0_1"/>
    <dbReference type="OMA" id="RIPHILM"/>
    <dbReference type="OrthoDB" id="407275at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095679" description="FAD-binding PCMH-type domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="8">
        <location>
            <begin position="68"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ83874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="517" mass="55920" checksum="CD943E6FE508BFB1" modified="2012-06-13" version="1">MAISTRMALLLTVCCFVPVPSFASSGGGGFLQCLSASLPRELVLSQDSPSFGSVLLSSIRNPKFSTPATVRPLCIVTPTNASHVQAAVLCGVRHGVRVRVRSGGHDYEGLSYRSERPEAFAVVDLARLRAVRIDSAAATAWVDSGATVGELYYAVAKAAPGLAFPAGVCASIGVGGHLSGGGIGMMMRKYGLSSDNVIDATIVDARGRILDKDSMGDDLFWAIRGGGGGSFGIVLSWKVRLVPVPPTVTFFNIQKTVDQGAVKAVTRWQTVAPALPEDLSIRVIVQPRQALFQSLYLGNCSALLRTMSSEFPELGMMRADCREMTWLQSTVYINSGDLKTPLESLLNRTTSLSTFTKNKSDYVKEAITEDSWEEIFPWFNRTSAGIIILEPHGGRVGSIADADTPYPHRSGVLYNIQYVAFWTRSGATDATNWISGLYDFMEPLVSKDPRGAYVNYRDLDIGENTVVGGVTSYDSGKVWGEKYFGGNFERLAITKGEVDAGDYFRNEQSVPPLVSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1J0D4</accession>
    <name>I1J0D4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_5g17760v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the expansin family. Expansin B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J0D4"/>
    <dbReference type="FunCoup" id="I1J0D4">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83950">
        <property type="protein sequence ID" value="KQJ83950"/>
        <property type="gene ID" value="BRADI_5g17760v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83950">
        <property type="protein sequence ID" value="KQJ83950"/>
        <property type="gene ID" value="BRADI_5g17760v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRTE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027462_1_2_1"/>
    <dbReference type="InParanoid" id="I1J0D4"/>
    <dbReference type="OMA" id="WLLMQES"/>
    <dbReference type="OrthoDB" id="406505at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22275">
        <property type="entry name" value="DPBB_EXPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005795">
        <property type="entry name" value="LolPI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF76">
        <property type="entry name" value="EXPANSIN-B15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00829">
        <property type="entry name" value="LOLP1ALLERGN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095724" description="Expansin-like EG45 domain-containing protein" evidence="5">
        <location>
            <begin position="26"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="6">
        <location>
            <begin position="53"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="7">
        <location>
            <begin position="175"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ83950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="26519" checksum="435C4D7709CAFB78" modified="2012-06-13" version="1">MVSGFQILSIAIAVLASLLCPCASSIEFHRKLSSWSSAGATWYGAANGAGSDGGACGYKGAVDQAPFSSMIAAGGPSIYGSGLGCGSCYQVKCSGNDACSGSVVTVVITDECAGGPCLNEPHFDLSGTAFGAMAKPGQADQLRGAGVLQIQYTRVPCSWAGAELTFVVDAGSNPHYFAVLVQYQNGDGDLSGVELMQGGAGGAAWTPMQHSWGAVWKLDAGSALQAPLSLRLTSSSGKKLVASNVIPVGWKAGAAYQSAVNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1J2D2</accession>
    <name>I1J2D2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100822597</name>
        <name evidence="13" type="ORF">BRADI_5g23350v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579360.1">
        <property type="nucleotide sequence ID" value="XM_003579312.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J2D2"/>
    <dbReference type="STRING" id="15368.I1J2D2"/>
    <dbReference type="EnsemblPlants" id="KQJ84862">
        <property type="protein sequence ID" value="KQJ84862"/>
        <property type="gene ID" value="BRADI_5g23350v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822597"/>
    <dbReference type="Gramene" id="KQJ84862">
        <property type="protein sequence ID" value="KQJ84862"/>
        <property type="gene ID" value="BRADI_5g23350v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822597"/>
    <dbReference type="eggNOG" id="KOG0156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_4_0_1"/>
    <dbReference type="OMA" id="FELIAYM"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11075">
        <property type="entry name" value="CYP77_89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000012">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051103">
        <property type="entry name" value="Plant_metabolite_P450s"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298:SF892">
        <property type="entry name" value="CYTOCHROME P450 89A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ84862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="56535" checksum="3188F24A80B858D2" modified="2012-06-13" version="1">MEDTAILTSPSLLLLCAASLLLLLLLIGRRRSHGRRPPPGPPALPFLAKFLLLRRSIFDLGPLLVGLHARYGPVISIRLFHRTYVFVADRELAHRALVQGGATFASRPPPHDPGRLFNAGGLDISASPYGPYWRILRRNLAAEALSPARVASFAAARRSTCDRLVSELAAAGAGSTVTLRPLLRRAMFGLLVRMCFGVELRPEELDEVEDLQRRALSAMTEFPVFSFFPALTKRLFHRQWAKLLAVRRRKDEVFGPLIHANRSVNISGNDSESPPFCYAESLLAVRVEDGRQLTDAEMVSLCSEFLNGGTDTTVTLLEWIMAELVNRPEIQAKLYQELQSRPPEDHNSGDLQSMPYLKAVVLEGLRLHPPGSFVLPHGVQQHLSPGAVAEIGGYTVPKGAAVNFLVAEIGRDEATWTAARKFRPERFMPGGEGHGVDVTGSREIKMMPFGAGRRACPGYALGIHHAEYFVARMVGELQWLPETEGKAVDMAEKLDFTTVMKHPLRARVVPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E118</accession>
    <name>A0A0Q3E118_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rx N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g01110v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81495.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E118"/>
    <dbReference type="EnsemblPlants" id="KQJ81495">
        <property type="protein sequence ID" value="KQJ81495"/>
        <property type="gene ID" value="BRADI_5g01110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81495">
        <property type="protein sequence ID" value="KQJ81495"/>
        <property type="gene ID" value="BRADI_5g01110v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E118"/>
    <dbReference type="OrthoDB" id="593438at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377">
        <property type="entry name" value="OS10G0134700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377:SF61">
        <property type="entry name" value="RX N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ81495.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="53020" checksum="5CA8279A2EA1753C" modified="2018-03-28" version="2">MEVAISAVAGELVSRFISFLMNKYNSSLSHAQSEEEGKVVERLQHLLMRAGTIVEEADARYITNSRMMMQLKILSEAMYAGYRVLDTLRYRTLQEGTRFDEVSIDNSSCGSLYLAIPSKRSRTTTQKDDGAFKSLEIAVANMAEFVVLLGGCERMSRRPYDVYLYTDNFMFGRHAEKQKLLSFLFQHNDPPGDIAPAVLPIIGGAAVGKKTLVAHACGDERVRSRFSSVLHLNGDSLLSHGMTMFGVTTLVVIEFASDVGDDDWKKGSKIIIVSRLQRLARFGSVKPIFPSALSYDELRFLFKALAFGSVDPTQHPRLAQLADEFARVLHITQGSLVTTNAFADALRRNLDVQFWSSILDKRIRMAKRNLSIYGVHPSMLIEQGHPVDITDLALHPLSMKGYNINVSIKERLPSVTFGKLLTDPSVRPKEDFILISWESRIPPHISFAHLVTSCAQDTHEGSNALPGRKRRVVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ECS1</accession>
    <name>A0A0Q3ECS1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC104584821</name>
        <name evidence="4" type="ORF">BRADI_4g00100v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the fantastic four family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238848.1">
        <property type="nucleotide sequence ID" value="XM_010240546.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ECS1"/>
    <dbReference type="STRING" id="15368.A0A0Q3ECS1"/>
    <dbReference type="EnsemblPlants" id="KQJ85550">
        <property type="protein sequence ID" value="KQJ85550"/>
        <property type="gene ID" value="BRADI_4g00100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584821"/>
    <dbReference type="Gramene" id="KQJ85550">
        <property type="protein sequence ID" value="KQJ85550"/>
        <property type="gene ID" value="BRADI_4g00100v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584821"/>
    <dbReference type="OrthoDB" id="1303570at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021410">
        <property type="entry name" value="FAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046431">
        <property type="entry name" value="FAF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33155:SF46">
        <property type="entry name" value="FAF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33155">
        <property type="entry name" value="FANTASTIC FOUR-LIKE PROTEIN (DUF3049)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11250">
        <property type="entry name" value="FAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAF" evidence="3">
        <location>
            <begin position="117"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="175"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="288"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ85550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="357" mass="39389" checksum="33053959D7B6E8E8" modified="2016-01-20" version="1">MTVAVMPALDAHTRIPAPSQLDVAWNSIFQTKPPPAMAPKASSMAMSNQSRLDLCTESLGCETGSHDDIMECLYARTVPEAEQPWEEPTQEEEEEEEEEVGDHGVLTAVRYHRAPRAFPPPLPSMSRRLQMRPVRRDGRLVVEAVATKPHGYLHARRQEGRLRLCFVDCRSVSDQSQSQSQKQSISPMQQQSMVPQEEEEDEDDEVKVVDRGTMVEVVAASGGKASRIVINKFVGGAPMSSDSDVQQPSPPVAAAADHPPELPGMRRVPSSTTTLAAAVAAAASSGMDDSDDDDDEEAESECETIKEKKKLVFSWRAGAGAGDREELVRSVRRCRQLRRHKQPLFILEPYYTINIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F1N5</accession>
    <name>A0A0Q3F1N5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100826173</name>
        <name evidence="7" type="ORF">BRADI_4g43760v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577100.2">
        <property type="nucleotide sequence ID" value="XM_003577052.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238780.1">
        <property type="nucleotide sequence ID" value="XM_010240478.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F1N5"/>
    <dbReference type="STRING" id="15368.A0A0Q3F1N5"/>
    <dbReference type="EnsemblPlants" id="KQJ92452">
        <property type="protein sequence ID" value="KQJ92452"/>
        <property type="gene ID" value="BRADI_4g43760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826173"/>
    <dbReference type="Gramene" id="KQJ92452">
        <property type="protein sequence ID" value="KQJ92452"/>
        <property type="gene ID" value="BRADI_4g43760v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826173"/>
    <dbReference type="OrthoDB" id="430207at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3F1N5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF80">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ92452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="251" mass="27161" checksum="AAFE44E528368B2F" modified="2016-01-20" version="1">MAMASALPLLLVSPRPASVARFRPRAAPLSTAAVALRKSGSRVAMLKPLRAASCCNPAAADAAAGGVAAVRAKGLQLVAWYLLSLDKNPVATKAVTSAVLTLAGDLICQLVIDKVPELDLKRTFVFTLLGLVLVGPTLHVWYLYLSKLVTMSGASGAISRLLLDQFIFSPVFIGVFMSLLVTLEGKPSLVVPKLKQEWFSSLIANWQLWIPFQFLNFYFVPQKLQVLAANFVALAWNVILSYKAHKEVIAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FF94</accession>
    <name>A0A0Q3FF94_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Agenet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100827174</name>
        <name evidence="3" type="ORF">BRADI_3g33240v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234996.1">
        <property type="nucleotide sequence ID" value="XM_010236694.3"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FF94"/>
    <dbReference type="EnsemblPlants" id="KQJ97782">
        <property type="protein sequence ID" value="KQJ97782"/>
        <property type="gene ID" value="BRADI_3g33240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827174"/>
    <dbReference type="Gramene" id="KQJ97782">
        <property type="protein sequence ID" value="KQJ97782"/>
        <property type="gene ID" value="BRADI_3g33240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827174"/>
    <dbReference type="OrthoDB" id="433924at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FF94">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014002">
        <property type="entry name" value="Agenet_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055274">
        <property type="entry name" value="SWO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48429">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48429:SF1">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00743">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Agenet" evidence="2">
        <location>
            <begin position="1561"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet" evidence="2">
        <location>
            <begin position="1650"/>
            <end position="1707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="767"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="901"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1163"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1497"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1715"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1784"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1832"/>
            <end position="1894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1907"/>
            <end position="2045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="654"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="806"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="850"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="874"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1224"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1514"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1715"/>
            <end position="1735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1751"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1848"/>
            <end position="1868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1874"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1910"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2002"/>
            <end position="2018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2031"/>
            <end position="2045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ97782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="2045" mass="218173" checksum="F29F49DE5F18C07E" modified="2016-01-20" version="1">MDYDDTDFQSQNFQLAGEDNSKSPSALRSFALPKLDIDDQLQGHLRFDNLVDSEGFFSAGGQDNSWIEVLSTGSSVVDFSASAAESCSISRTNYVWSEATSTECVEMLLKSVGENEMTGSMDGSAHQQLSGMDSQIDLSNTQPKSSNSPSGSIVVPTENDQSQSKSSEKLSSASKNTTESCPAVGNYFDVVHADDSLDNLSIHSAGVDSGKLNDEPFSDLAPIQNMYSTGSYHFQQDNQGSGVGVTHQDSEIQCMHKNKLEGGIHELQKMSESSDGLLEAITNPVKMMQRDDDTCKRASAPLQPSFSQVDAANEPETSVDTSDKLVIEKFGMGEEPSSAKSRRSQPDMEHSNSHVITALSTESGQLIQSPNGKQLTHVTGVPEETKGGGLDDTNTDVSKLGVLEQHQESVHSLKTVIMDDTDINTGEDSKLGVPEQHQDSVDNLNSVVMEEKITREEISAVSGDFEHLVETGHDENAKSATGTSKDEFDLSGDVTTGNSSAGLLDETDPNILSVNHQGPVKEDDTPALENEPVNQHLVSPNSVPSSINISSTTVADTSNTSMDKLDCSGGVPSGGSPSGILDGNDLMTESFKKGASSSLEVGDNNVTSPVSEPWGKNLAIPVNSNIDAIHSSNTDSVASKTQCEEQATLTMNQTDDKSGDHPDSYTQKCQTDGPSLQSEHQENVHLQKFQIDEPPIQSEHHGISVTPSSLGVSPDKAAEAIIETPLNAKDDQSAHIKDIDRSCNDATCGSPTVISCTEPCVQEGGQGGNDLLRQTPSEQSGDQKDPAASADIFQSSKECSARNIEPTISSEETNTAGDDRGFSFEVGDPPEVSEKAHCPAWSPFPISKAAQPQSTEASTETPKTGNPSGNALRTIEDSKRKTGKEQLPGKKVVESVGVLSISSHNGDGTKTRSTPLEQPQQHPTTESTALAHQPFTDIQHVQLRAQIFVYGALIQTIPPAEAYMVSAFGEHVGGGKPTWEAVWRVAVERFQIQKSPLIGLETPTSSRIGSRVAEKASKGLAVKTAPASKKGDKTVSLAHPATLLHSPTFNASPLGSSTLNLQRGSHLDFSQAVSPLFAYNSQTRQPTSAVASWFPQSAGPRAAPWLVPPQNLIFDSSMQPTAPSSESAKGSFQNISISQAITPGVFLPNQASATVASPLAVVHEEKQKTPASTSKRGRGGAASRKPRKRKKASASPEQELDINNSQLKTDIASVTPATDHTPGFTLSTRSTSSALGGGPIPNTSLITSVPDYLVGKGAEKTIIFSEQISGAVEQSMDQAKGASMSSMEALRHSEVVWSHLSKNSRSQLPAEVEQKLNSAAAAASAAVSVAQAAAEAAKMAAAAALQAKLMTEEALGSSKSANSLQNRHAGEIDVNNNLASLSSLTPKSSWKMNDSSHAPGSTISVAREVARKRVEEASAAAKRAENLDAILKAAELAAEAVFKAGSIIGMGEPLPFTLSELLEAGPDGYWKSDRVKNKKAGNTSVNAVIEEVEVPNDINKSGRKSRSKAKIDKATQNSEQSSSVKGLQPDGMQSGNGVGNDPAAAPLNDSRNDTSPSIIWNGIGKGSLVEVLADEGAFKAAWFCAKVLDINEDSACISYETHGEGTGIREAWVSLKQDGEKAPQIRLCHPATMPKSKGTRKRRRDTPGNYSWAIGDHVDAWIENSWREGIISQNCESGEIKFVVQLSVGDSMVVDAWNLRPSLVWKDGQWTEWSRAQERKDKSNNKGDSPYEKRQKTAVCDPVPTVGVAPEPHKDKGTKNTTKPEEQPLALSDKDIMFNIGKSAVENKSTRRPGLQKEGSKVFGVPKPGKKKKFMEVSKHYVADQADKISEGNASTRFAKHLMPQVSRPRESTSKPDQRGTRIGDMRSRGLKSAKSQNVSTNSIPGKDPPSMPVLSSAVLESSFAFAGSMPSSSNTANPTMERNNPAHGTGLRTENVSLPESRIPAAPTGPATKKNSTATDRAKRKYVPSVDSNLNRRMLKTSEIPGQTTSDFAEPRRSNRRIQPTSRLLEGLQSSLIISKVPGEKAPRTNYRSATSTSRGKAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FL83</accession>
    <name>A0A0Q3FL83_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g48900v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FL83"/>
    <dbReference type="FunCoup" id="A0A0Q3FL83">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK00368">
        <property type="protein sequence ID" value="KQK00368"/>
        <property type="gene ID" value="BRADI_3g48900v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00368">
        <property type="protein sequence ID" value="KQK00368"/>
        <property type="gene ID" value="BRADI_3g48900v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FL83"/>
    <dbReference type="OrthoDB" id="686404at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994:SF10">
        <property type="entry name" value="OS04G0508700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994">
        <property type="entry name" value="OS04G0515000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK00368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="24987" checksum="14F905D00A75AE34" modified="2016-01-20" version="1">MVAISDQGEEQPVLPRNGGHTDTTKLDVCVTLFASMILLALVLVFFFAIIFILLSPILHDPAAYSMGIVAVSGLDPTTNRSASALLDPEFNLTVRVASSSLNVPWPWFGDDCLDPSTSVEVSYLRVPLAGARAPRNVCTRAWHSPKDRSFVARGRGVDVPGFLVDSLAEDMRRGEAVFEITLMTPHRQGWVVLTCWAKVGDASSTLETPCAKSSVNAGLPMIQLAGLLGLFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FVV9</accession>
    <name>A0A0Q3FVV9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1618 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g08362v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03519.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FVV9"/>
    <dbReference type="FunCoup" id="A0A0Q3FVV9">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FVV9"/>
    <dbReference type="EnsemblPlants" id="KQK03519">
        <property type="protein sequence ID" value="KQK03519"/>
        <property type="gene ID" value="BRADI_2g08362v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03519">
        <property type="protein sequence ID" value="KQK03519"/>
        <property type="gene ID" value="BRADI_2g08362v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FVV9"/>
    <dbReference type="OrthoDB" id="589927at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FVV9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011676">
        <property type="entry name" value="DUF1618"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33086:SF73">
        <property type="entry name" value="OS01G0245901 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33086">
        <property type="entry name" value="OS05G0468200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07762">
        <property type="entry name" value="DUF1618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1618" evidence="1">
        <location>
            <begin position="238"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03519.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="431" mass="47375" checksum="0633C8B90251F3AB" modified="2018-03-28" version="2">MAGRLLFFAAHLKTLSPITSRGIRNATSTIRHFTTTSGTGHGEMASASPSWVILGAVPRVAAADADLPPGADLSLALPAPPRVALLTIPPRMFPGRITTDRFPSVVAADASGLLLLKADQGRATGPTIIDTPPRKEFSWRPFVEGYFVLDAKTASAFPLPKPELILHPGHLGLIASPTSYGRHYMVAELQPLHVTHQATLMRFSSEVGEWAGTEVGFPLPPRILSPNGVVSHSGRLWWIDLSWCLLTCDPFVDEPRLRVVPLPEGKALEDREAWGFLDKYRCVSVSGGKLRFVDMYMNHNSRGAAQISMWTLADPDSTETEWTLEYEATFAEIFDDASYKATELSRKTPVLALVHPTNCDVVYFFQDEHLLGVDVPARKVVECEMYQLVAPPSKHLATRFVHAWQLPRALCSGSAKENVVGVNEEVEQLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FYB5</accession>
    <name>A0A0Q3FYB5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100839801</name>
        <name evidence="11" type="ORF">BRADI_2g07076v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FYB5"/>
    <dbReference type="STRING" id="15368.A0A0Q3FYB5"/>
    <dbReference type="EnsemblPlants" id="KQK03317">
        <property type="protein sequence ID" value="KQK03317"/>
        <property type="gene ID" value="BRADI_2g07076v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03317">
        <property type="protein sequence ID" value="KQK03317"/>
        <property type="gene ID" value="BRADI_2g07076v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FYB5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF59">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER ABCD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="114"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="456"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="8"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK03317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="748" mass="83434" checksum="955AD39040201140" modified="2016-01-20" version="1">MCCCFPCTSSSQQLPPLPRPQPLRPPSRSASPRVGLPLPLQAVAASSMRRRGRRRAAPPPPTAAAGDASTDSSGPGGEEKRDGTDLKTLARRFWKVAAPYWWSEDKVQARLRLAAVFTLTLATTGISVGFNFLGRDFYNALADKDQEQFTKQLLYYLGGFAVGIPFFVLRDYARETLSLRWRSWMTSYYMKRYFKNRTFYKIQSQSLIDNPDQRINDDLSSFTGTALAFALTLLNAVVDLVSFSNILYGIYPPLFIVLVVYSLGGTAISVFLGKNLVNLNFMQEKKEADFRYGLVRVRENAESIAFYGGEENELQLLLARFRRAYQNLTELLVASRNLEFFTNGYRYLIQILPAAVVAPMFFSGKIEFGVINQSVSAFNHILSDFSLIVFQFQSISAFSAVIDRLGEFDDLLDANEPSLSSRRDSIDGINIFFKSGSPSVLSSNGSVMQSDPGIVLEICNLTLLTPRSENVLLTDFSMELKDKDHLLVMGPSGSGKTSLLRALAGLWTSGTGNIVYHARDSAELQTSNLGSDKPSNIKPEGEELLQSSKQRRDNGIFFVPQRPYMVLGTLRQQLLYPTWTEKIHQSPINDAQKTDLPFLSEVNTSDGVGAESEMPSTEELIRVLEAVRLGYILPRFNGLDSMHDWASVLSLGEQQRLAFARLLLAKPTLVLLDESTSALDDTNEVHLYSQIEAAGITYISVGHRKTLHRFHNKVLYISKSDSTTSSLRNWELKPTDQKSIEESSPFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G2W0</accession>
    <name>A0A0Q3G2W0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100825399</name>
        <name evidence="2" type="ORF">BRADI_2g21350v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566097.1">
        <property type="nucleotide sequence ID" value="XM_003566049.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G2W0"/>
    <dbReference type="EnsemblPlants" id="KQK05639">
        <property type="protein sequence ID" value="KQK05639"/>
        <property type="gene ID" value="BRADI_2g21350v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825399"/>
    <dbReference type="Gramene" id="KQK05639">
        <property type="protein sequence ID" value="KQK05639"/>
        <property type="gene ID" value="BRADI_2g21350v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825399"/>
    <dbReference type="OrthoDB" id="695018at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3G2W0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34193:SF21">
        <property type="entry name" value="OS05G0493200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34193">
        <property type="entry name" value="OS11G0199801 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK05639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="179" mass="18821" checksum="41D68023648D4C6D" modified="2016-01-20" version="1">MMEPQSARARRIARSREEMMGLLADFSAGGGEEEDSDRELSFSDLVEAIGQPSAAADHVSKEEKEEEDVVASAKRQAAAAAMAGKEVRRLRRRRSNGRGSGGGDGVLLNFYVPGLLSRSMTAPRSGRGALPTAAAASSGAGHGVPARAAAGKSRMQAPLAIGCWPALWGRGRDHRNKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GU34</accession>
    <name>A0A0Q3GU34_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g15868v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14383.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GU34"/>
    <dbReference type="EnsemblPlants" id="KQK14383">
        <property type="protein sequence ID" value="KQK14383"/>
        <property type="gene ID" value="BRADI_1g15868v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14383">
        <property type="protein sequence ID" value="KQK14383"/>
        <property type="gene ID" value="BRADI_1g15868v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GU34"/>
    <dbReference type="OrthoDB" id="694273at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GU34">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK14383.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="14795" checksum="E9698CDB285A3192" modified="2018-03-28" version="2">MCSICATEQEDGYHAVMNCTKARALRDSVRLVWSLPPDAALRRTGPDWVLLLLSQVDEDCRSKLLFLWWRAWHLRNDVIFAKGDASVSASAQFLFGYANSLLSLKDKIKAPDLKGWVKLNVDASFIPASGLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GW15</accession>
    <name>A0A0Q3GW15_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g21334v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15224.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GW15"/>
    <dbReference type="STRING" id="15368.A0A0Q3GW15"/>
    <dbReference type="EnsemblPlants" id="KQK15224">
        <property type="protein sequence ID" value="KQK15224"/>
        <property type="gene ID" value="BRADI_1g21334v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15224">
        <property type="protein sequence ID" value="KQK15224"/>
        <property type="gene ID" value="BRADI_1g21334v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GW15"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF970">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK15224.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="234" mass="26060" checksum="10EED63BFAD6A2B5" modified="2018-03-28" version="2">MQLQRFNRSTARQKEKNTTGHVTPFTALASALLHAPGGIIHVTLVHTEHNRRRLLRTHGPAALCSSPNNDDGRLRFETIPDGLPPSDSLDATQDIWRLAEATRRACPAHDRDLLRRLNAQESGGSRPPVSCVVADGAMGFVAHVAKEVRLPAYLFFTQSACGLLGYLHFGQLVQRSLVPFKGRAQTRRCDAAKCYITQLRLKLTCPGSRTRQARMMRRRSDRKEEGDAPGLLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GWW1</accession>
    <name>A0A0Q3GWW1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box associated beta-propeller type 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g23365v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GWW1"/>
    <dbReference type="FunCoup" id="A0A0Q3GWW1">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK15511">
        <property type="protein sequence ID" value="KQK15511"/>
        <property type="gene ID" value="BRADI_1g23365v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15511">
        <property type="protein sequence ID" value="KQK15511"/>
        <property type="gene ID" value="BRADI_1g23365v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GWW1"/>
    <dbReference type="OrthoDB" id="587254at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006527">
        <property type="entry name" value="F-box-assoc_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF13">
        <property type="entry name" value="F-BOX PROTEIN CPR30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07734">
        <property type="entry name" value="FBA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box associated beta-propeller type 1" evidence="1">
        <location>
            <begin position="87"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK15511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="378" mass="42711" checksum="56B27D3378A2F469" modified="2016-01-20" version="1">MPLQLNTSSTTRLYGMPLQPTRIVSFDFSARGEHGSAGHHHHHPPQLPPRALWFTSPLFTLPVGSWDGVVCLRRGSSPDPHGIGFRDQQYVLWNPVAMACATVRAPQQRLAGYIVGGYAHPGTGRFHLLHASCRETHGSIRISPTLLRILRVGDDAWRELPFPKIVMNTCNVVRLHDSLHWLAQPSSGSETYWMESALPLQVLVFDTTREKFWLMETPAELAERPLREAQLGVLSKKLCIFTVEPSSSTMEVWVLDGYGSGGATPRRRCTSWRLEERIGLITWDGYDLSRVITTVHLQVDVGENEEEILVTHVHGRIDAYSAGRKAWRHAGNLRTSFLAVEFLVMHRESLVRGQVSFGKATRDLLKYVDHYGQRFYCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HF36</accession>
    <name>A0A0Q3HF36_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g58953v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HF36"/>
    <dbReference type="EnsemblPlants" id="KQK21141">
        <property type="protein sequence ID" value="KQK21141"/>
        <property type="gene ID" value="BRADI_1g58953v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21141">
        <property type="protein sequence ID" value="KQK21141"/>
        <property type="gene ID" value="BRADI_1g58953v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HF36"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK21141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="133" mass="14898" checksum="F62B9DCAE0A3D203" modified="2016-01-20" version="1">MLISPSALPISHPVYLLHEQPYLLPHQRHCPSSLTVIPSPPPTPAAGDDRARMEEQQRRRVRISPSLSFFSSKSHFLSRRFLGWWPARRAILASNKLAGLFDLPTRSLLDQRLEDKRLAGPGAAAARGAWRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HGQ2</accession>
    <name>A0A0Q3HGQ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Integral membrane bound transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100831379</name>
        <name evidence="9" type="ORF">BRADI_4g45370v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92666.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577182.3">
        <property type="nucleotide sequence ID" value="XM_003577134.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HGQ2"/>
    <dbReference type="STRING" id="15368.A0A0Q3HGQ2"/>
    <dbReference type="EnsemblPlants" id="KQJ92666">
        <property type="protein sequence ID" value="KQJ92666"/>
        <property type="gene ID" value="BRADI_4g45370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831379"/>
    <dbReference type="Gramene" id="KQJ92666">
        <property type="protein sequence ID" value="KQJ92666"/>
        <property type="gene ID" value="BRADI_4g45370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831379"/>
    <dbReference type="OrthoDB" id="68611at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HGQ2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049453">
        <property type="entry name" value="Memb_transporter_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30509:SF9">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN MDTO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30509">
        <property type="entry name" value="P-HYDROXYBENZOIC ACID EFFLUX PUMP SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13515">
        <property type="entry name" value="FUSC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Integral membrane bound transporter" evidence="8">
        <location>
            <begin position="501"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="428"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ92666.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="851" mass="92533" checksum="2852DD15FA07DA77" modified="2018-03-28" version="2">MGMGMADGCSNKQNVRLEMAAAPSSSSRGRSMAGRRWRSSMASGLRAALACTIVGVVSVYAPPALLRHITFPAFSYVVTVIIITDDATVGAALRAVASAAHATAMGAVPSVLALWLAQRMGESSSTTSSVLGTSALVALSAFAVAVPESPGPVAKRIALGQIIIIYVAKFRQLPTTNNGLGVVVQHPANVVACTALGAAAALLAVLLPWPRLATREVEEKSVAYMENAAERVRLLVDAFLLRATVTAEEEEDEEEEEVTSGRRRRRWCVAACMSEAHRLASASAALLRRITSVKGDLQWERVVRLGAAGTMPAADEQERIEMPIKGMEIAVSSTNYLPRPAADQAEMTIINLSCLEQMRDQIRLSLLTATSTSHHITKMNNNNKRSTTMSLFGGAAAERHELELSPFLFLFSMHLLRHPTLLLSHSLPDSTNKVTPACPQLEEPDQDDQQEDQQDSEPEEESKMSTDKEQKKKCRTLLVRRWGLQSSRLKMASKCAVSLGLAVLLGLLFNNDHGFWSGLIVATTMAPAARGSTWAVAVARAHGTAIGSVYGVLACLLSQQRHLMELRFLALLPWIVLATFLKRSRAYGPAGGVAAALSGIIIVGRRYDEPPMAFTISRLVETFIGLSCTVATDLAFQPKAMPSARARTQLPRCFAALRDCLARLPSLRKNQQEQHKMLLEQVALLGKYAAEAGAEPNFLWMAPFPASCYAKVHGSLSRMAQLLGLYLHAQAIIVDNTSYGSQLLAGTDVKRFHNRLSASLPELELDEEDTDFDLEDGNGKWCEDMAVVVKSFIGHAREALLQEEEEEEEQHQQLNAYCLGSIGFCMGEMMKEAQQLEALMLDLSLQLQPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HI93</accession>
    <name>A0A0Q3HI93_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial import inner membrane translocase subunit Tim21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_1g67861v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TIM21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HI93"/>
    <dbReference type="FunCoup" id="A0A0Q3HI93">
        <property type="interactions" value="992"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3HI93"/>
    <dbReference type="EnsemblPlants" id="KQK22528">
        <property type="protein sequence ID" value="KQK22528"/>
        <property type="gene ID" value="BRADI_1g67861v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22528">
        <property type="protein sequence ID" value="KQK22528"/>
        <property type="gene ID" value="BRADI_1g67861v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HI93"/>
    <dbReference type="OrthoDB" id="436405at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HI93">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.320">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit Tim21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013261">
        <property type="entry name" value="Tim21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038552">
        <property type="entry name" value="Tim21_IMS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032:SF6">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08294">
        <property type="entry name" value="TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK22528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="264" mass="29826" checksum="ED59B7A8BE70CBB6" modified="2016-01-20" version="1">MASRITRLPYHQQRHLLATAAEASSHRLPRARGAIPKYTISEKFIFVHVKYNQLRDIFACLLPPLDGFSLKNSRWYMIKSSPSNPLTMRECRKVFPSLIRPSASYSTQASDQKTQKERKDLSTTEDPFDDAPTYNIPEKPVTFAEGASYSLVILAGLGIFGKALAKVQSDSQVTARIGYPITGYGSETRNRAARQRVPSRIWTDEEGVEHVEVNFYIRGPHGAGKVYSAMFKDNSDRSWKFTYLLVEFTAPHQGQVMLESYIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IBX4</accession>
    <name>A0A0Q3IBX4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g15810v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IBX4"/>
    <dbReference type="FunCoup" id="A0A0Q3IBX4">
        <property type="interactions" value="1781"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3IBX4"/>
    <dbReference type="EnsemblPlants" id="KQJ83616">
        <property type="protein sequence ID" value="KQJ83616"/>
        <property type="gene ID" value="BRADI_5g15810v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83616">
        <property type="protein sequence ID" value="KQJ83616"/>
        <property type="gene ID" value="BRADI_5g15810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821890"/>
    <dbReference type="OrthoDB" id="1840940at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF9">
        <property type="entry name" value="AUXIN-RESPONSIVE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ83616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="131" mass="14612" checksum="D04B6D3A601F7769" modified="2016-01-20" version="1">MKGGRSNSNANRRLLRSFLSACRKLQLLGAGGDWARLGSGDEDQAKGGIPWDVPRGHTVVYVGEELRRHVVRVSSLGHPLFRELLDRAGEEYEFAGANRLCLPCDEDFFLGVLCHVGDSKQVHHWRLSLCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IUN2</accession>
    <name>A0A0Q3IUN2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_2g11931v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04140.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IUN2"/>
    <dbReference type="STRING" id="15368.A0A0Q3IUN2"/>
    <dbReference type="EnsemblPlants" id="KQK04140">
        <property type="protein sequence ID" value="KQK04140"/>
        <property type="gene ID" value="BRADI_2g11931v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04140">
        <property type="protein sequence ID" value="KQK04140"/>
        <property type="gene ID" value="BRADI_2g11931v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IUN2"/>
    <dbReference type="OrthoDB" id="6161812at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IUN2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF999">
        <property type="entry name" value="NB-ARC DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="168"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="426"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="542"/>
            <end position="874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK04140.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="938" mass="106444" checksum="C119715A955B9B3B" modified="2018-03-28" version="2">MEGVMASVVTGAISSLLTKLATLLENKYNLSKGVKKEITFLRDEMSSMNALLVKLSRMEELDEQQKDWRDKVRDMSYDMEDCIDIFTNDLDSGEASAVLLSGLKKLQARYKIANRIEELKARVMEASSRHNRYKLDEHIGSPRGLVTIDPRLQALYAEADSLVGIDGPKDKIIELLRMEENTQKLKLVSVVGFGGVGKTTLAKQVHDTIKSHFDRTAFVSLSQNPTMVKVLSDILSGVAGWMPSFPKEEHQLIDELRRRLKDTRYLIVIDDIWSMDVWNIVKCSFVENNRGSRVITTTRIEDVAQACCSCFHGHVYKIKPLNDLDSRRLFHKRIFPSKDSCPEQLKNISNEILKKCQGVPLAILSVASILASHEEVNSNEIWEKIHNCFVFQLEGNPALQWMRHVFNLGYNDLSLDLKTCMLYLGIFPEDSEITKADLVKRWIAEGFISEKHGYGPEEIAESYFSELINRNMIQIGGFDDCGNVSSCRVHDLMLDFITLNKRIPRIHDPDTTKGCITEVRRLSIQLRNSECNHVLGNMALTQVRSFNFWGPGQFRPSLSRFQLLRVLHLDVSDSKEHLSSLRSFFQLRYLRISGVVCTGLLKQLQTLKHLKTLEVAHCVDDDVDFRELPSMLWHLIVPRSVVLRGGIGRMESLRTLDAPSLDLKDVGILKRLGDLNNLRELKLDLGILLWDILLGGPSGFSSDLLLSSLGRLGSLQSLTISGFCIHDDILAYWSPPPRHLHRLHVLECPFSVVPDWIAQLQNLRSLEIQVVSLGKDGAEVLASLTSLVNLKMHVRDHAPEEGISVRDATFPNLKEFQFRYKLPCLMFEAGAMPRLQNLTIDCYAQALRQGSDGVLDGIQHLGSLKVFNVDIYEQEGFSHGQLHVQPNFVREEEEEVPRMQEEEVGRWNLQSLKAAFRQAINKHPGNPHVHISTLRMNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IVZ4</accession>
    <name>A0A0Q3IVZ4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g30096v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IVZ4"/>
    <dbReference type="EnsemblPlants" id="KQJ90208">
        <property type="protein sequence ID" value="KQJ90208"/>
        <property type="gene ID" value="BRADI_4g30096v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ90208">
        <property type="protein sequence ID" value="KQJ90208"/>
        <property type="gene ID" value="BRADI_4g30096v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IVZ4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ90208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="101" mass="9737" checksum="58EC5E325204EA5A" modified="2016-01-20" version="1">MAVLAAARQCGDKTARGPCQGRDGRDAGRAQTGIVMAVTAATRGSGEGSRRRPQGKGRRRGVGAGEAGCAAGQSRAAGGGGGGGGCRGREGGGGGRSRLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KAC5</accession>
    <name>A0A0Q3KAC5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Wall-associated receptor kinase galacturonan-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_2g38295v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07857.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KAC5"/>
    <dbReference type="FunCoup" id="A0A0Q3KAC5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3KAC5"/>
    <dbReference type="EnsemblPlants" id="KQK07857">
        <property type="protein sequence ID" value="KQK07857"/>
        <property type="gene ID" value="BRADI_2g38295v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07857">
        <property type="protein sequence ID" value="KQK07857"/>
        <property type="gene ID" value="BRADI_2g38295v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3KAC5"/>
    <dbReference type="OrthoDB" id="667709at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33491">
        <property type="entry name" value="OSJNBA0016N04.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033725008" description="Wall-associated receptor kinase galacturonan-binding domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase galacturonan-binding" evidence="4">
        <location>
            <begin position="42"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK07857.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="450" mass="49498" checksum="294D9F4EB7A17D62" modified="2018-03-28" version="2">MTARSPAPVVMLLLLLATMASSSFVGARAVVEPEEAIGMPNCDITCGDLKVPYPFGMGPSRCYWPGFKITCDHRTGKHPRLLLANNGNNGTLQQQQLQVQELYLTYPFLLVTRSPAAGGTIDGMPFKLWSGKNQLTLTGCNVRATLKNGTPPCCSSVCETYETTTAPYALTRDSLPMPCSGTHCCQADIIVNRQHRRRRLLRRQQLGYLTSYHVELTYLSWNRRSDEARVPTRVFLADKGWFEHVWLASNETTTATAEDHYQVPIWLQWEVVGDGVEPAYRRNTTAYRCPEEAAARVCKSKHSACRKGTIGYTCSCHEGFQGNPYVTHGCKAISPSSPPLSSPCLGNAGNRRPPPCLVRLIPLPPDCLGPALRRRILLRPTAGPPAATGSAAAGSRAQEGEVCRRVCRRHGVPSSRRAWRRVRRRRVEEVPAAASCLLHSGSPCRGCATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KG32</accession>
    <name>A0A0Q3KG32_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC112270547</name>
        <name evidence="2" type="ORF">BRADI_2g51463v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313963.1">
        <property type="nucleotide sequence ID" value="XM_024458195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KG32"/>
    <dbReference type="EnsemblPlants" id="KQK10001">
        <property type="protein sequence ID" value="KQK10001"/>
        <property type="gene ID" value="BRADI_2g51463v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112270547"/>
    <dbReference type="Gramene" id="KQK10001">
        <property type="protein sequence ID" value="KQK10001"/>
        <property type="gene ID" value="BRADI_2g51463v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:112270547"/>
    <dbReference type="OrthoDB" id="1681423at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="646"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="704"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK10001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="738" mass="81518" checksum="BAE0AAB82A97AD61" modified="2016-01-20" version="1">MGNNNVTGQQVSEESAKGFEETIDGTDWLPGLDNINDKEKDHREYADVSESLKGTIISNGGGEQMALDQSNLSTKNKDLDEKNRCSHQDHTSMQDDDQNINLPTEQGDETQEDNETETTTNSTEKYIHVSTTIEDMASSKDTSPSNEKLKQLVHETAEGSEQSTLANPHRLLQENIEGPPGDEKESWSQEVAVMCGNLPADTVESLQETKIGVSTVDNLLTPWPIQGESTSLTESIISGYLDAETKEIVIKDEPGERDNSSYVRAEDGTNQEKSKKHNAGIPEEKQAICEMRKSCETITSETVIEMLEEDKKIHGLENHEEEFPKCSAFGALDIGQVVSIFQSSLTDPSATNGKEFQNHELKKSIAATGQMTDSVSARMEHNLIEKTNTEQDGTVDKAAVVKNLAEDLEPSKKFDGVPDLVSLLEVNGKDFTSLYPPLSDPFPILNEEKVQKDVMAEELAVPMTASALQLVQDSVKKGYMKHDSSDNNEAKTTDTQDTVNISQCAEPQQILLEEPEVVKFEDSETLSICTKLYRSTTGLTSCREYNMEREGAYATAECFTSESSQEKVTGTAGFTPESKQMKVITHADKATEEWCLFQKPSLGRAASEEIPLLQNAENFNSLSYSSEQHIKVAEDIPMKDITLMQSKDEAEEEPENSPLLGPREPSEGTFRALNHSARNNKPLTSLMTEDQVGTLPLLKEQEHVQKKITTVSSPRSKERKKSQSSIFASCICCASNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KPJ6</accession>
    <name>A0A0Q3KPJ6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g07635v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KPJ6"/>
    <dbReference type="EnsemblPlants" id="KQK13041">
        <property type="protein sequence ID" value="KQK13041"/>
        <property type="gene ID" value="BRADI_1g07635v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13041">
        <property type="protein sequence ID" value="KQK13041"/>
        <property type="gene ID" value="BRADI_1g07635v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3KPJ6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK13041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="176" mass="18269" checksum="8A74D38A1AEFB583" modified="2016-01-20" version="1">MLSHASLTPFLPSPVASPSPAGLCLHGLPYHPGASTLAAAGSVHYAVRSVLSATTGSGLSATGAGLAATGFFARGSATQGREAGAQQEGRWTSAAAATREKMRRHGGEEKTSRDERRRAGEENEGRGDSGTSSFAAWVDSKKMQGHFCKKSGLAGLFGTEGIGSWTQFWVEYNLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KS37</accession>
    <name>A0A0Q3KS37_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="11">dUTPase</shortName>
            <ecNumber evidence="4 11">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100837014</name>
        <name evidence="14" type="ORF">BRADI_1g12830v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in nucleotide metabolism via production of dUMP, the immediate precursor of thymidine nucleotides, and decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>dUTP + H2O = dUMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the dUTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="4 11"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KS37"/>
    <dbReference type="FunCoup" id="A0A0Q3KS37">
        <property type="interactions" value="2219"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3KS37"/>
    <dbReference type="EnsemblPlants" id="KQK13841">
        <property type="protein sequence ID" value="KQK13841"/>
        <property type="gene ID" value="BRADI_1g12830v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837014"/>
    <dbReference type="Gramene" id="KQK13841">
        <property type="protein sequence ID" value="KQK13841"/>
        <property type="gene ID" value="BRADI_1g12830v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837014"/>
    <dbReference type="OrthoDB" id="10261072at2759"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3KS37">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.40.10:FF:000004">
        <property type="entry name" value="Deoxyuridine triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001862">
        <property type="entry name" value="PRK00601.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241:SF0">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="13">
        <location>
            <begin position="96"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK13841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="225" mass="23932" checksum="B6146BBD0B9BD4BA" modified="2016-01-20" version="1">MHTGAGKLYGGRIWRHLFHFPPRRLPIYLPEQPRRLNSSNQTPPPQLPFSRSRRPRIPTSIAMAATNGAAADSVQEPPHKITKIAPLLKVKKLSEKAILPSRGSASAAGYDLSSAADAVVPARGKALVPTDLSIAIPEGTYARVAPRSGLALKHSIDVGAGVIDADYRGPVGVVLFNHSEVDFVVKPGDRIAQMIIEVIVTPEVAEVEDLDATVRGEGGFGSTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KYH2</accession>
    <name>A0A0Q3KYH2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC112269400</name>
        <name evidence="12" type="ORF">BRADI_5g25113v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the WUS homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311863.1">
        <property type="nucleotide sequence ID" value="XM_024456095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KYH2"/>
    <dbReference type="FunCoup" id="A0A0Q3KYH2">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3KYH2"/>
    <dbReference type="EnsemblPlants" id="KQJ85141">
        <property type="protein sequence ID" value="KQJ85141"/>
        <property type="gene ID" value="BRADI_5g25113v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112269400"/>
    <dbReference type="Gramene" id="KQJ85141">
        <property type="protein sequence ID" value="KQJ85141"/>
        <property type="gene ID" value="BRADI_5g25113v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:112269400"/>
    <dbReference type="OrthoDB" id="773671at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090506">
        <property type="term" value="P:axillary shoot meristem initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044555">
        <property type="entry name" value="WUSCHEL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45940:SF2">
        <property type="entry name" value="WUSCHEL-RELATED HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45940">
        <property type="entry name" value="WUSCHEL-RELATED HOMEOBOX 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="14"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="16"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ85141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="295" mass="31826" checksum="D5913D6E5E4DB07D" modified="2016-01-20" version="1">MDGAAANGGQAAPCRASGTRWTPTAEQVRILRELYYGLGIRSPNAEQIQRIAGRLRQYGRIEGKNVFYWFQNHKARERHKKRLTTIDVSPNNNDSNNNCSNNASILSLSPSSGAAAGLYGAGSNGGGGSAHLQMDANASATCWDVGSNAAMANDRSFMQLLQEQDYMGVRTSTAAMAPTPWPACFPAFSPYQPPPAREPETLPLFPTGGGSSGGHQEIVNGVHGGSYQLLQSNSQQLCWGQQHHHHHQLLLQEQQNYQYSSYSSNNQLMMPTQDAAAAASLELTLSSHYPAGSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LGA3</accession>
    <name>A0A0Q3LGA3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100841891</name>
        <name evidence="14" type="ORF">BRADI_4g39250v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578725.1">
        <property type="nucleotide sequence ID" value="XM_003578677.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LGA3"/>
    <dbReference type="FunCoup" id="A0A0Q3LGA3">
        <property type="interactions" value="888"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3LGA3"/>
    <dbReference type="EnsemblPlants" id="KQJ91701">
        <property type="protein sequence ID" value="KQJ91701"/>
        <property type="gene ID" value="BRADI_4g39250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841891"/>
    <dbReference type="Gramene" id="KQJ91701">
        <property type="protein sequence ID" value="KQJ91701"/>
        <property type="gene ID" value="BRADI_4g39250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841891"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LGA3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051657">
        <property type="entry name" value="RNF168/RNF169_E3_ubiq-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23328">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23328:SF0">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="154"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="53"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="227"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="259"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ91701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="398" mass="42934" checksum="9EAB54A1CC82FFF2" modified="2016-01-20" version="1">MAAESPKSAPRRAAAAAAKNGAGLGLLSPRFLSAAEMAGWDEESLLLAALVVEDTPVRESRRKRRPSPAGGSAGSNTRKRRSRRPPPGEIPPVMLALDDEEEKPNNPADGKKEIEDEQKKAVVVETKQPSRSGKEAVPAGELPCMDRLREELSCAICLEICFEPSTTPCGHSFCVKCLKHAASKCGKRCPKCRQLISNSRSYTINTVLWNTIQLLFPSEVEARRSSTASCSASKDEVKKQNPPRSNTNLVQSDLRRSRNTGGSVSSITTQDNTNTRISSNASRRSFTTQGIRRSTTTNNTGSFVPQDTSGITGRTGGRSFVQASQLVATSRVGSANPPSSDDDAALAYRLQQEEFATAFESEEGGGETQRRNAVSAARDSLRAMASRAVRLRARGWPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3M1X1</accession>
    <name>A0A0Q3M1X1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100841964</name>
        <name evidence="5" type="ORF">BRADI_3g37252v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235181.1">
        <property type="nucleotide sequence ID" value="XM_010236879.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235182.1">
        <property type="nucleotide sequence ID" value="XM_010236880.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317621.1">
        <property type="nucleotide sequence ID" value="XM_024461853.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317622.1">
        <property type="nucleotide sequence ID" value="XM_024461854.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317623.1">
        <property type="nucleotide sequence ID" value="XM_024461855.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317624.1">
        <property type="nucleotide sequence ID" value="XM_024461856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3M1X1"/>
    <dbReference type="EnsemblPlants" id="KQJ98501">
        <property type="protein sequence ID" value="KQJ98501"/>
        <property type="gene ID" value="BRADI_3g37252v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841964"/>
    <dbReference type="Gramene" id="KQJ98501">
        <property type="protein sequence ID" value="KQJ98501"/>
        <property type="gene ID" value="BRADI_3g37252v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841964"/>
    <dbReference type="OrthoDB" id="607373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000381">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000288">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g02750"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001228">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g20770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000073">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF506">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT-LIKE SUPERFAMILY PROTEIN ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="109"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="210"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="312"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="382"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="515"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="616"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="831"/>
            <end position="923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ98501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="923" mass="102921" checksum="2856E7E99EB29F93" modified="2016-01-20" version="1">MPDDHRTPSSWYTAISGCVRCGRDSTAFSMLRGMRERGVPLSGFALASLVTACERWEEGRACGAAIHALTQKAGLMVNVYIGTALLHLYGSQKHVLDAQRLFWEMPERNVVSWTALMVALSSNGHLEEALGYYRRMRRERIACNANAFATVVSLCGSLEDEVAGLQVFSHVIVSGLQRQVSVANSLISMLGNLGRVHDAEKLFYRMEERDTVSWNALVSMYSHEGLCSKSFRVFSDMRRGGLLRHDATTLCSLISVCASSDYVSYGSGVHSLCLRTGLHSYIPVVNALVNMYSSAGKLADAEFLFWNMSRRDLISWNTMISSYVQNGNNMDALKTLGQLLHTNEGPDRMTFSSALGACSSPGALMDGRMVHAMTLQLSLHHNLLVGNSLITMYGKCNSIEDAERIFQLMPNHDVVSCNILIGSYAVLEDGTKAMQVFFWMRRGEVKLNYITIVNILGSFTSSNDLRNYGLPLHAYTIHAGFLSDDYVSNSLITMYAKCGDLESSNNVFQRIINRSVVSWNAMIAANVQHGHGEESLKLFMDMRHDGNGLDHICLAECMSSSASLASLEEGMQLHGLGLKCGLGNDSHVVNAAMDMYGKCGKMDEMLKMLPDPAIRPQQCWNTLISGYARYGYFKEAEETFKHMISVGRTPDYVTFVTLLSACSHAGLVDKGIDYYNSMSSVFGVSPGIKHCVCIVDILGRLGRFAEAEKFIEDMPVLPNDLIWRSLLSSSRTHKNLDIGRKAAKRLLELDPFDDSAYVLLSNLYATSARWSDVDRVRSHMKTINLNKIPACSWLKQKKEVSTFGIGDHSHKHADKIYMKLDEILLKLREVGYVADTSSALHDTDEEQKEQNLWNHSEKLALAYGLITVPEGCTVRIFKNLRVCADCHLVFKLVSMVFDREIVLRDPYRFHHFKGGSCSCSDFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3MIX4</accession>
    <name>A0A0Q3MIX4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g13035v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3MIX4"/>
    <dbReference type="EnsemblPlants" id="KQK04334">
        <property type="protein sequence ID" value="KQK04334"/>
        <property type="gene ID" value="BRADI_2g13035v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04334">
        <property type="protein sequence ID" value="KQK04334"/>
        <property type="gene ID" value="BRADI_2g13035v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3MIX4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3MIX4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK04334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="182" mass="20408" checksum="6C464C28BF52AE30" modified="2016-01-20" version="1">MQNSPLFRHGKDSSISSHREPRASTAACLPLARAPSRACLRPCPRLAQHRLLPSFRTGGDRGEMPRSHPPPSTAATFPHHAWSPINRLTSDDHPATRTRRDYFTTEHQEHAAIAKSHPPPPPFLNSAKFLILIHMRSEIVAQSMTTTTEMHSDQLERKKASERLGLGGEMCSTKRKNWINAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3N0T3</accession>
    <name>A0A0Q3N0T3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100833366</name>
        <name evidence="5" type="ORF">BRADI_2g52330v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in translation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3N0T3"/>
    <dbReference type="STRING" id="15368.A0A0Q3N0T3"/>
    <dbReference type="EnsemblPlants" id="KQK10150">
        <property type="protein sequence ID" value="KQK10150"/>
        <property type="gene ID" value="BRADI_2g52330v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10150">
        <property type="protein sequence ID" value="KQK10150"/>
        <property type="gene ID" value="BRADI_2g52330v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3N0T3">
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    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000042">
        <property type="entry name" value="Protein translation factor SUI1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
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    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="4">
        <location>
            <begin position="33"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK10150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="117" mass="13092" checksum="A083804272863290" modified="2016-01-20" version="1">MSDLDVQLPSAFDPFAEANAEDSSAGAGSKDYVHVRVQQRNGRKSLTTVQGLKKEYSYNKILKDLKKEFCCNGTVVQDSELGQVQCCNHILLIKFPWCKCVYTDERSAIFSGHSTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3N614</accession>
    <name>A0A0Q3N614_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hyaluronan/mRNA-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104583610</name>
        <name evidence="3" type="ORF">BRADI_1g01860v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234622.1">
        <property type="nucleotide sequence ID" value="XM_010236320.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3N614"/>
    <dbReference type="FunCoup" id="A0A0Q3N614">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3N614"/>
    <dbReference type="EnsemblPlants" id="KQK12150">
        <property type="protein sequence ID" value="KQK12150"/>
        <property type="gene ID" value="BRADI_1g01860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583610"/>
    <dbReference type="Gramene" id="KQK12150">
        <property type="protein sequence ID" value="KQK12150"/>
        <property type="gene ID" value="BRADI_1g01860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583610"/>
    <dbReference type="OrthoDB" id="686299at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3N614">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039764">
        <property type="entry name" value="HABP4/SERBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006861">
        <property type="entry name" value="HABP4_PAIRBP1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12299:SF71">
        <property type="entry name" value="HYALURONAN_MRNA-BINDING PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12299">
        <property type="entry name" value="HYALURONIC ACID-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04774">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="252"/>
            <end position="344"/>
        </location>
    </feature>
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        <location>
            <begin position="29"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="320"/>
        </location>
    </feature>
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            <end position="567"/>
        </location>
    </feature>
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        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="478"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK12150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="567" mass="60670" checksum="85C59F2ABE393EF5" modified="2016-01-20" version="1">MGSYFDLLDLAEGASGEEAVAKVVGKVVGKVVPPSEPKPPKKKPGLKGAQPQPQQDGMEEYSGDHGGNYDNNADHGGNYHGNGDRGGYYNGNGDRGGYYNGDGGRGGYSNGNGGRGGYRNGNGGRGGYRNGNSGRGYHNGNGGRGYNNVGYNENNSHYQQGAYGGEYYGYGDMQAQGGNYHHSGERQWYSRDGGQARKNRNLQYKPKSKAASVASSDPDLKPEEQAEPAVETKQQDASADNADGVPAPESEKSSGDVAKDDSKKEGEGGTKKKDGVKTKSMCGSARKRQVNKKKAMEANADGSKKTIGNEEEVNQEEKKEMTLEEYEKMLKEKKSLEPAKLEGREINPVAFEGLLKLEKKLEDDADGEHKEKEVVVKNVKPRKSMTIQEYLKPADGQEYVPPRPPRRDGPFNSSYRGGRGNGGFSDRSRDNSTESRVYNSGRGDNAIVFHNVDATSGNAAPRSGGRPDHNNGRGNGGYQERQGGYNGGYQERQGGYQQGGYNGSRGRGGYRQGGNSEGYGNGGYQHGGGGRGYGGCGRGRGYPQPRPVPRPDSAAHFPALTGSSPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RXK8</accession>
    <name>A0A0Q3RXK8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g34454v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RXK8"/>
    <dbReference type="EnsemblPlants" id="KQK17434">
        <property type="protein sequence ID" value="KQK17434"/>
        <property type="gene ID" value="BRADI_1g34454v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17434">
        <property type="protein sequence ID" value="KQK17434"/>
        <property type="gene ID" value="BRADI_1g34454v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3RXK8"/>
    <dbReference type="OrthoDB" id="593438at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377">
        <property type="entry name" value="OS10G0134700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377:SF16">
        <property type="entry name" value="RX N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK17434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="24069" checksum="0E49CF0E67771B41" modified="2016-01-20" version="1">MSGMILVIVEFVSDVADEDWNKFHSFVKKTNRRSKVIIVSRHQRIARFGSVKPIFLNPSKNLQPGGSLLTVNALADVPRMNLNVQFWLCMLNTGRRVIQKNLSEHGVPLPPYLRFQYGHSDITDFALHASSPIRITPWRSSITNDLTKTESPKVVTFRELLVQHAERPKEEFSLLAWESRMPPYTKFAHMVASSTDQDLPQGDVTLSGRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CG55</accession>
    <name>A0A2K2CG55_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histidinol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g09046v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CG55"/>
    <dbReference type="STRING" id="15368.A0A2K2CG55"/>
    <dbReference type="EnsemblPlants" id="PNT61007">
        <property type="protein sequence ID" value="PNT61007"/>
        <property type="gene ID" value="BRADI_5g09046v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61007">
        <property type="protein sequence ID" value="PNT61007"/>
        <property type="gene ID" value="BRADI_5g09046v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CG55"/>
    <dbReference type="OrthoDB" id="1750324at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT61007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17196" checksum="7BBFDB72AE2028B8" modified="2018-03-28" version="1">MGSLRAPALPVGGASAGRGCRLCLASSRVLKSKVLTVSCAMKSYRLSNLSDVEVSSLKARPRIDFSSIFSTVNPIVEDVRVRGDAAVKDYTEKFDKVILDNVVVRVSDLLDAELDPAVKEAFDVAYDNIFAFHVSQKMTEKTVENMKVVLQCCLQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CJA8</accession>
    <name>A0A2K2CJA8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_5g25691v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT62110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CJA8"/>
    <dbReference type="STRING" id="15368.A0A2K2CJA8"/>
    <dbReference type="EnsemblPlants" id="PNT62110">
        <property type="protein sequence ID" value="PNT62110"/>
        <property type="gene ID" value="BRADI_5g25691v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62110">
        <property type="protein sequence ID" value="PNT62110"/>
        <property type="gene ID" value="BRADI_5g25691v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CJA8"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CJA8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11075">
        <property type="entry name" value="CYP77_89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051103">
        <property type="entry name" value="Plant_metabolite_P450s"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298:SF825">
        <property type="entry name" value="CYTOCHROME P450 89A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="489"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT62110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="62667" checksum="CE92C15139ABFAC8" modified="2018-03-28" version="1">MEAAVAFFLLVPLATCAALILFLLLLLPAVKPSKTGGRSLLPLPPSPPSNPILGPLLWLWRARSGLEPVIRAVHRHHGPILTLFFLSPWPAIFVSGRAATHRALVQLGPTFASRPPAIAPFPVLTSAQRTVSSAPYGPLWRSLRRNLAAGVLLRSRHALYSPARRWALALLFSGLDNGGEGKAVVTVVEPLQRAMFALLSSMCFGRRLDDDAVRGIQAVQRELFANYIGFQVFAFCPALTTRVLFRRRWRKVLDIRRRQEQLFLPLIRARRDRRSSSNGEAADDGDVAVYCYADTLLAHRLPKEEEEGGEPRGLTEGEMVSLCTEFLTASVDTTVAALQWVMANLVLYPEIQSKLHHEIAAAMTISNKDHDDHAVVCEEDLENLPYLKAVVLEGLRRHPPAHFLLSHAVSEEEEDAEAACLYGYRIPAATSVNFSVANVGMDEEIWSRPEEFRPERFMAGGEGDGVDLTGGSRPEVMRMMPFGAGRRICPGMALALLHLEFFVANMVWGFEWAPAAGEGGGVNLAERPEFTVTMERPLRALVKPRRRSRRRPDSSYVQSLIVRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CLR3</accession>
    <name>A0A2K2CLR3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104584436</name>
        <name evidence="3" type="ORF">BRADI_4g10003v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237401.1">
        <property type="nucleotide sequence ID" value="XM_010239099.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CLR3"/>
    <dbReference type="STRING" id="15368.A0A2K2CLR3"/>
    <dbReference type="EnsemblPlants" id="PNT62963">
        <property type="protein sequence ID" value="PNT62963"/>
        <property type="gene ID" value="BRADI_4g10003v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584436"/>
    <dbReference type="Gramene" id="PNT62963">
        <property type="protein sequence ID" value="PNT62963"/>
        <property type="gene ID" value="BRADI_4g10003v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584436"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043158492" evidence="2">
        <location>
            <begin position="19"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="460"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT62963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="531" mass="54699" checksum="2371969B71F748B0" modified="2018-03-28" version="1">MRTGLSLAILLLAAAASADDLGYGGGGAEKKAEVVAEKKAKVADAYAVEKKPEAVAEKKAEIVAEKKAEAADAYADGVKKPEAVGEKKAEVVAEKKAEVKAEKKAEVKADAYAEKKAEKKAEKKAEVKAEKKADAYTEKKAEVKAEKKAEVKAEKKAEVKADAYAEKKAEVKAEVKAEKKAEVKAEKKAKGKSDAYAEKKAEKKAEVKAEKKVKGKVEAAKPEAGKAEKKAKHEKREKAKKAESKAPDAYAAPKAEPKKEEPKAPVAITPDAYAAPTKEEPKTPASTPEVPKKEEPAATTPVTPNKEEPKAPAPAAGTPDAYAAPKAKAPAPAAGTPDAYAAPKKEEATAPAPAAPKEEAKALAGTPDAYAAPKKEEAPAPAAGTPDALKKEVASAPALAAGTPDAYAAPKKEETPAPAAAATAPAAGTPDAYTVPKKEESKETTPAPESAVTEKKAEPETPAAATTDAYAAPKTAEPATPAAPMTTDAYAAPKTTKPETLAAPATTDAYAVPGAGADAWHKKLNPFSARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CR48</accession>
    <name>A0A2K2CR48_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g29284v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CR48"/>
    <dbReference type="EnsemblPlants" id="PNT64483">
        <property type="protein sequence ID" value="PNT64483"/>
        <property type="gene ID" value="BRADI_4g29284v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64483">
        <property type="protein sequence ID" value="PNT64483"/>
        <property type="gene ID" value="BRADI_4g29284v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CR48"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT64483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="189" mass="21066" checksum="FFF1B5AA1F0BDBB7" modified="2018-03-28" version="1">MSTSMRVHEILFVGEVHASEVDGDDSAVELVRRDVGPQTRCSGVRASATRRHAREGHLCTVNLLLDNRPAYTLQDTEADPIDPKGASARVAAVNRAALVGAFGRTLRQLECWMRQRQWEGVAGGELTELAECAAGRRPEARQSCPARWRHRGGCKRDKERRGGCARNHFLQKMDRDAMCFLFYGIWSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CTB3</accession>
    <name>A0A2K2CTB3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g39381v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CTB3"/>
    <dbReference type="EnsemblPlants" id="PNT65257">
        <property type="protein sequence ID" value="PNT65257"/>
        <property type="gene ID" value="BRADI_4g39381v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65257">
        <property type="protein sequence ID" value="PNT65257"/>
        <property type="gene ID" value="BRADI_4g39381v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CTB3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CTB3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT65257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="251" mass="27984" checksum="8092CA3B084D54AA" modified="2018-03-28" version="1">MPLAWSSRTASSRCRRNGPQAVRRRAASRMVADPNGRRRQRDPTSPRTHPRPPAGGRIRSQPPCGSRPPGSSSSNTRLPSPVALGHGLATAGSPSGRPSVADSASPTLPAQKASPDSQYGHRRHRAAPNRSCHRRVIRSADFRKKTTRYYKGIFHALSTICRDEGGKLLYRSWATLLDMTSCTMVVNLLHDITEGMASDELITSKVVTMFVYEGNVTCLMMTYLICFYAYYYIWLWCLYSCAVYLCLLGFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CVT9</accession>
    <name>A0A2K2CVT9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g07752v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT66147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CVT9"/>
    <dbReference type="EnsemblPlants" id="PNT66147">
        <property type="protein sequence ID" value="PNT66147"/>
        <property type="gene ID" value="BRADI_3g07752v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66147">
        <property type="protein sequence ID" value="PNT66147"/>
        <property type="gene ID" value="BRADI_3g07752v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CVT9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="91" mass="10548" checksum="C0960BC77623F868" modified="2018-03-28" version="1">MATDGSHSQSTLEKTRKKNRVSNVNMNEASVLFRREYFPDLNLNICLDEMYESTTYCNTCIELSVFRQLNYMNLVLPKTEQCNQIVALTIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CWJ0</accession>
    <name>A0A2K2CWJ0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_3g11042v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CWJ0"/>
    <dbReference type="EnsemblPlants" id="PNT66401">
        <property type="protein sequence ID" value="PNT66401"/>
        <property type="gene ID" value="BRADI_3g11042v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66401">
        <property type="protein sequence ID" value="PNT66401"/>
        <property type="gene ID" value="BRADI_3g11042v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CWJ0"/>
    <dbReference type="OrthoDB" id="662529at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CWJ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055312">
        <property type="entry name" value="FBL15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="132"/>
            <end position="252"/>
        </location>
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    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT66401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="422" mass="47082" checksum="477D33997396674E" modified="2018-03-28" version="1" fragment="single">MGASSDRISSLPDDLIILILTHLGSAAAAARTSVLSRRWRRIWTHLPELFLNEPAATSDSIDAALAAAYSAGGTLRLLAITMPRDANAARVSPWLRFASQRLAGELSLDVNLRLTGATEPEEGSRKLELPPCERVTEMDVCFNYYTLGLPHDRISFPALAVLNMSCAKIEAQDLERLGSLPRLRELVLRGITLATVYDVSIRSDTLERLIYLVSRTRRLEIVAPKLQALRTYSSSGPDHEIAEAYVVAPSLADLAWFFYDPQRHRFVGAGRHLRKLSLLDSSTTALMQRFDTADELEIKLSMDWVREGYENFMNAMSKPTKCKVLDLQLRYRGLPSHFHAPCVLYLLKKSSGLRRIVVHLPLGNMVVIMNFEEGNNHVEFLSLLLGCNTPILKTVVINSETRALSKDTCIKIRSNASPIIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CWJ7</accession>
    <name>A0A2K2CWJ7_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BRADI_3g11005v3</name>
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    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT66394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="BRADI_3g11005v3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT66394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="153" mass="16967" checksum="7A3D9FD4D91998E8" modified="2018-03-28" version="1" fragment="single">LVQPYFFQRNNRPDPSRFPLLPPWRRARPPHRRAGGGSSQLPSSLPSKSLPRPTPPPLPAPRGHAQRGRLPPLPVHRDHARRGCPTPPTSPSRPRPSPLPGKILSPARAPPARWCCVYILPLNGFLPPLTHLQSHHIFDLEDAGLSAPQSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
    </protein>
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    <organism evidence="1">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g15932v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g15932v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CXD8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT66702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="113" mass="12678" checksum="FE19B28F56AE5912" modified="2018-03-28" version="1">MMIQRTTMAQPSSSFSTSSFTTGCCCWLLALLCLFDSLRRSWPLRAMASTASYTIVSLQAWSCQYYLVDWFLLCRLDGASGQVLVGHECRLAGWRGSQGSGMSRHQKFRIAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYJ0</accession>
    <name>A0A2K2CYJ0_BRADI</name>
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            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">BRADI_3g20674v3</name>
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    <organism evidence="1">
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CYJ0"/>
    <dbReference type="EnsemblPlants" id="PNT67089">
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        <property type="gene ID" value="BRADI_3g20674v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67089">
        <property type="protein sequence ID" value="PNT67089"/>
        <property type="gene ID" value="BRADI_3g20674v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CYJ0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT67089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="154" mass="16907" checksum="31DC28566EE24F88" modified="2018-03-28" version="1">MSRVLRRCLGAIPPQERPVAASMPTEPDDSSFLHPSRRSKALCAAMLLSGGRGGEDSASVPRVVSGQFLVPGPFPAVFWSRSVTVWRVAGSRLRVGGQTWSASADFWRWLVFLMGGAVLVVFHPLRRPCCGGVVVNWWSSSARIRLWITVNFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYM7</accession>
    <name>A0A2K2CYM7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100845198</name>
        <name evidence="6" type="ORF">BRADI_3g21431v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CYM7"/>
    <dbReference type="STRING" id="15368.A0A2K2CYM7"/>
    <dbReference type="EnsemblPlants" id="PNT67132">
        <property type="protein sequence ID" value="PNT67132"/>
        <property type="gene ID" value="BRADI_3g21431v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67132">
        <property type="protein sequence ID" value="PNT67132"/>
        <property type="gene ID" value="BRADI_3g21431v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="696486at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF122">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="65"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT67132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="324" mass="38123" checksum="8326AAA76466E2CB" modified="2018-03-28" version="1">MCTGTDADSVPCKKELFRLIYNSPLYLRNGGKQLEINRFPVSALERRYYIVLCRLGHSERYQCDIAIMYRKVRCSYRSLGQSLMPGGHVNNFLISVFCRKLFDDCHPSISKKHFFFSYIGENILKYNSRDQFKLIRNAFKGASLAMKIDACELLFFPICHCEHWFLFVVDLQNCLFAFMDSLYSKKSRYQIVVRSLLIGNFKHLWREVVDPEYNFDNFRIVYPAIPRQGNGHDCGIFVMKCMEIWTPGVVLHDYFSKVNIPNIRIQYANQLFFSSNNTVDKSLVTDFFREKEYNFTSKRVPYNEEIDIAYIGKSEKHSVDIVWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D4I5</accession>
    <name>A0A2K2D4I5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Remorin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g50721v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the remorin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D4I5"/>
    <dbReference type="FunCoup" id="A0A2K2D4I5">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT69176">
        <property type="protein sequence ID" value="PNT69176"/>
        <property type="gene ID" value="BRADI_3g50721v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69176">
        <property type="protein sequence ID" value="PNT69176"/>
        <property type="gene ID" value="BRADI_3g50721v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D4I5"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D4I5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005516">
        <property type="entry name" value="Remorin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471">
        <property type="entry name" value="OS02G0116800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31471:SF53">
        <property type="entry name" value="OS02G0658400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03763">
        <property type="entry name" value="Remorin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Remorin C-terminal" evidence="4">
        <location>
            <begin position="305"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="94"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="317"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT69176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="413" mass="45312" checksum="4DEA3FAF8A1E3584" modified="2018-03-28" version="1">MRRLDHPLPLPLPCVGEPTAASRVSPGSSPARSDASEGAAAFYAADDEPDPEPAASVGRSTQMLLAMAAMGGRGGPYGRRPASSYGSCAAWSAGSLTDHRPASPSPICSPGSSKGGDGCQDGGERRDGGDDDASSFVTSREEEEEQERLPTRGDFAKLCATPRNIRLQTPRHPSLLDRRVDGSNRGPPRFVHKATPARLMRRARSSHNYHGRRLGAIDAVNEWRLPKVSEEEDEAVDQNDWQADTVSSRISSARDWNFEPDGVYEENNQGGRAFGNSDGEDCPVAVQRMERRLRGSAVKPKENFVQAKLVAWKDAQITKLIDKLKRKEANIDDWQKSKITLARNEMTKTEMKLEKKRAEAVQKMQKAIKQAQKKADNKKIKEQAATANQIAGVERALVKMSRTGKLPWSLAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D542</accession>
    <name>A0A2K2D542_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g54515v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D542"/>
    <dbReference type="EnsemblPlants" id="PNT69386">
        <property type="protein sequence ID" value="PNT69386"/>
        <property type="gene ID" value="BRADI_3g54515v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69386">
        <property type="protein sequence ID" value="PNT69386"/>
        <property type="gene ID" value="BRADI_3g54515v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D542"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT69386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="141" mass="16463" checksum="2AF1EC78C69DF5FE" modified="2018-03-28" version="1" fragment="single">PHARAIWWPKLFSPSRYFSSSPLLPQEVFAIAVLRTPHLSRARLDLPPDGGGGGRRWRCSRREATAERDRRGDAMERDQARRSGMRCGPWRRCSRREVAAEREQARRRCWSKHGAAAEMDEVRGRRGIWLYICGIKLVRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D5N8</accession>
    <name>A0A2K2D5N8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_3g58237v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="325" mass="38408" checksum="21670B50D2B2990B" modified="2018-03-28" version="1">MRAALEIEMPETTHLWCKWHVLRKAQESLGPVYTQSGVFRDRFYKVINEMLTVDEFEKAWDDLIDEYDLRKNTFMVRTYEARHMWAKTYSKDKFCLHVERNSSMNHFVEQYNIMLQNRAMEEDTEEHKTKQVKKLSKYMWPVEYHAYKIYTCATYNLFCREIDKASRYVIARNSAGPLYEVCHAYPEKRERWARVKFQVAVIADGESYTCECGLYTHFGILCCHVIRLLNFLGVNEIPKAHIMKRWTKDARDVLPPHLAFYQKDSPTMQSRTFRNRVLVQNSLDLAVKGDTNIDTYEIVMKHIAAANKELEPIIAAAHTGHRTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
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                <person name="Shu S."/>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    <sequence length="236" mass="26310" checksum="534630030C50544B" modified="2018-03-28" version="1">MSKHSRNFPAAGYEKSLTPARIPPAGNVSRGWLNGAAAFSVEVKSESALHRADDGGAVHGRPDGREQRTPRANVPSQTRTRRGIHPSVTSHGTPPRCRRRRVLLVCCWWPNAEVRACARGSEAKSHPAFLLFLFQRPTKKRATSLGGGEPGCLREVFFSFLALSDRPRFAIFLFCPLCCLVLSVWPRGRGWLPCILQSHVPVRNADTCFGTSLKSYYYRRGESCVSRVRRIDAKVH</sequence>
</entry>
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            <strain evidence="4 5">Bd21</strain>
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    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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        </citation>
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            <strain evidence="5">cv. Bd21</strain>
        </source>
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    </dbReference>
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        <property type="entry name" value="Vps5_C"/>
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    <dbReference type="PANTHER" id="PTHR46757:SF1">
        <property type="entry name" value="OS05G0474500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46757">
        <property type="entry name" value="SORTING NEXIN-RELATED"/>
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    </dbReference>
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        <location>
            <begin position="98"/>
            <end position="216"/>
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            <begin position="25"/>
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            <begin position="428"/>
            <end position="455"/>
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            <begin position="25"/>
            <end position="39"/>
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            <begin position="59"/>
            <end position="70"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="EMBL" id="PNT71064.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT71064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="508" mass="56017" checksum="E6AD3C8679571BF1" modified="2018-03-28" version="1">MAASTSPPAASVCAAGDDLETLALDSASSSPAAVSTDPLLRPPSSPAAAANHEPFVIDDFLDDEEDDDDIPPSPSPRAPAAPAEAPPEFDRITVSDPKKHAEPVAGAAGVIPGSGSYFSYLVTTSLVGGGGGEFRVRRRFRDVVALADRLAATHRGLFVPTRPDKSLVEGQVMQRHDFVNQRCTALQRYLRRLAAHPVVGRSSDLRTFLTQPGAIPAFEGEAPRYWTTTANVATMPVPAKAGRDLFGMFKDLKQTVVNGLVATKPPPVEEETDTEFLSHKARLQDLQQQLTTTSQQAESLVKAQDDLRTTTAHLGMTLIKLSKFEREQATCDSHRRRSSDINNFATSVVKFSRSQAKLNSEIVKRLDNIHEYLEMMISVQHAFTDRSNALQHVQSLSADLFFLHTRAGRLESVSSRGIGQEWTRYQKIEGLKETISAAEAAKSQALREYESIKENNMIEIARFDKDRRRDFVDMLKGFVIKQVIYSDNFANMWGKVAEETQVYANRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D9Y8</accession>
    <name>A0A2K2D9Y8_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g22979v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
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            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
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            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="123" mass="13535" checksum="D327F3DAC3A79927" modified="2018-03-28" version="1">MGEASEGGGGSRRIWGRTGRRQQEDVGNALRQRQVGRGRRRRQVSPDPIGEATHGLGTSAADLTESVRGTDVWAGNGRRSRRIRLGHQRGRGTAADPGNNEAEARSSGSWQRGSLQRFMGRWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DDU8</accession>
    <name>A0A2K2DDU8_BRADI</name>
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            <fullName evidence="5">Zinc finger GRF-type domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="2" type="ORF">BRADI_2g44854v3</name>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT72465">
        <property type="protein sequence ID" value="PNT72465"/>
        <property type="gene ID" value="BRADI_2g44854v3"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="595554at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT72465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8441" checksum="3FD56697AAF49928" modified="2018-03-28" version="1">MSSGSVSSGGRRWGRGTVGNRHSSVPYREDPMEYELTKCCLCARREAPRWISWSPRNPGRRYYACVDALISSISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DFJ6</accession>
    <name>A0A2K2DFJ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100842530</name>
        <name evidence="10" type="ORF">BRADI_2g52695v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753614.1">
        <property type="nucleotide sequence ID" value="XM_014898128.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DFJ6"/>
    <dbReference type="STRING" id="15368.A0A2K2DFJ6"/>
    <dbReference type="EnsemblPlants" id="PNT73052">
        <property type="protein sequence ID" value="PNT73052"/>
        <property type="gene ID" value="BRADI_2g52695v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842530"/>
    <dbReference type="Gramene" id="PNT73052">
        <property type="protein sequence ID" value="PNT73052"/>
        <property type="gene ID" value="BRADI_2g52695v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842530"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="6 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT73052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="58499" checksum="304A3E26A8D6C0C3" modified="2018-03-28" version="1">MEPSLTSGLAMLLLLLLPLLCFLCLYWDAKKQTRADGLKAYPIIGTLPHFVKNQHRLIEWTAGVLARCPTYTMSFNFRGLGLTAGVMTANPANVEYVSKTNFQNYPKGEFVVSVIGDFLGHGIFNSDGDHWLWQRKAASYEFSKRSLRNFVVSTVRFELVERLLPLLSRAERDGRTLDVQDVLERFTFDNVCCVVFDEDPACLTEEGLGANGRAEFMLALTEAQNIVMARFMAPVKWAWRVKKLLNMEPERRMRQALATIHGYVDRIVRERSERGAAGLARKDDFLSRFASSGEHSDESLRDVVTNFIVAGRDTTSSALSWFFWMLSSRPDVEDKILQEIHALRRASSSTGAGDDAAAFGYEELHGMQYLHAAITESMRLYPPVAMNTHCSMRDDVLPDGTFVGRGWQVTYNAYAMARLEEIWGGDCSEFRPERWLDEEGVFRPASPFKYAVFHAGPRMCVGKEMAYIQMKSIIATVLERFSFRFVGCDTRPGLVFSLTLRMEGGLPMQVKKRGALNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGS2</accession>
    <name>A0A2K2DGS2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleotide-diphospho-sugar transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g58994v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGS2"/>
    <dbReference type="STRING" id="15368.A0A2K2DGS2"/>
    <dbReference type="EnsemblPlants" id="PNT73478">
        <property type="protein sequence ID" value="PNT73478"/>
        <property type="gene ID" value="BRADI_2g58994v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73478">
        <property type="protein sequence ID" value="PNT73478"/>
        <property type="gene ID" value="BRADI_2g58994v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DGS2"/>
    <dbReference type="OrthoDB" id="604820at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DGS2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044821">
        <property type="entry name" value="At1g28695/At4g15970-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46038">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46038:SF9">
        <property type="entry name" value="OS01G0920800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="1">
        <location>
            <begin position="38"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT73478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="280" mass="32391" checksum="24533D66B67CCC2B" modified="2018-03-28" version="1">MEDKTVIITTVNEAWARPGSLLDIYLESFKNGEDTEHLLAHVLIVALDPAGFRRCTVVHPHCHLLEVKIANLTSATPFMSKEYLELVWTKLYLQQCILELGYNFLCTDTDMILLRDPFRRIPVYADMSVSSDDFSSARAPLDNPPNTGLYYMKATNRSIEMLRYWQAARPRFPGVNDQPVFVKIKTELIEKLQVRIEPLDTVYFGGFCEYHDDFDKICTMHADCCIGVDNKVHDLMDVVADWRRYRSMTLEERKNTSANLTWTVPLLCRKSTNWHKPVHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DH73</accession>
    <name>A0A2K2DH73_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g61305v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DH73"/>
    <dbReference type="EnsemblPlants" id="PNT73626">
        <property type="protein sequence ID" value="PNT73626"/>
        <property type="gene ID" value="BRADI_2g61305v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73626">
        <property type="protein sequence ID" value="PNT73626"/>
        <property type="gene ID" value="BRADI_2g61305v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DH73"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT73626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="59" mass="6402" checksum="40B61A32C356480E" modified="2018-03-28" version="1">MAVGCPAVKEKRLLNALAAPRHKPQATQDDPRHKPQATQDDPRHKPQAFAASDFAGTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DJ56</accession>
    <name>A0A2K2DJ56_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g12309v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DJ56"/>
    <dbReference type="EnsemblPlants" id="PNT74304">
        <property type="protein sequence ID" value="PNT74304"/>
        <property type="gene ID" value="BRADI_1g12309v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74305">
        <property type="protein sequence ID" value="PNT74305"/>
        <property type="gene ID" value="BRADI_1g12309v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74306">
        <property type="protein sequence ID" value="PNT74306"/>
        <property type="gene ID" value="BRADI_1g12309v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74304">
        <property type="protein sequence ID" value="PNT74304"/>
        <property type="gene ID" value="BRADI_1g12309v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74305">
        <property type="protein sequence ID" value="PNT74305"/>
        <property type="gene ID" value="BRADI_1g12309v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74306">
        <property type="protein sequence ID" value="PNT74306"/>
        <property type="gene ID" value="BRADI_1g12309v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DJ56"/>
    <dbReference type="OrthoDB" id="677464at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33165:SF49">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
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                <person name="Shu S."/>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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</entry>
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    </evidence>
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    </evidence>
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    </evidence>
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</entry>
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                <person name="Shu S."/>
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                <person name="Vogel J.P."/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_1g45505v3"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DPI3"/>
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        <property type="component" value="Chromosome 1"/>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
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        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76185"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DR62</accession>
    <name>A0A2K2DR62_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g53265v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DR62"/>
    <dbReference type="EnsemblPlants" id="PNT76773">
        <property type="protein sequence ID" value="PNT76773"/>
        <property type="gene ID" value="BRADI_1g53265v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76773">
        <property type="protein sequence ID" value="PNT76773"/>
        <property type="gene ID" value="BRADI_1g53265v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DR62"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT76773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="106" mass="11430" checksum="EB85235B1BE33909" modified="2018-03-28" version="1">MAGKGVARLLHKVRPWRLGRGKAKEAYGTQWMASACRPWQLRGRDEMGGDGGRWDSASIPVHGVELEMVKGQPEMMGRPEVTLLRGFYPSVAGRVALVAGGVLGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DR93</accession>
    <name>A0A2K2DR93_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_1g53656v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DR93"/>
    <dbReference type="EnsemblPlants" id="PNT76798">
        <property type="protein sequence ID" value="PNT76798"/>
        <property type="gene ID" value="BRADI_1g53656v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76798">
        <property type="protein sequence ID" value="PNT76798"/>
        <property type="gene ID" value="BRADI_1g53656v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DR93"/>
    <dbReference type="OrthoDB" id="696608at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="15"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="57"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT76798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="102" mass="10955" checksum="026D631529071B9C" modified="2018-03-28" version="1">MEEAEAELGEVFYLCSFNPSPVEVVTHFLPRLISGEPLPRGAEHLIHRVDIYNHEPKDLAPATASSSRSPNARKGAAPGQRASPAPAPGQSRRQGTSTMNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DSL9</accession>
    <name>A0A2K2DSL9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100827338</name>
        <name evidence="10" type="ORF">BRADI_1g60395v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561566.1">
        <property type="nucleotide sequence ID" value="XM_003561518.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DSL9"/>
    <dbReference type="EnsemblPlants" id="PNT77275">
        <property type="protein sequence ID" value="PNT77275"/>
        <property type="gene ID" value="BRADI_1g60395v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827338"/>
    <dbReference type="Gramene" id="PNT77275">
        <property type="protein sequence ID" value="PNT77275"/>
        <property type="gene ID" value="BRADI_1g60395v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827338"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF627">
        <property type="entry name" value="OS06G0192800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043158758" description="RING-type E3 ubiquitin transferase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="46"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT77275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="148" mass="15228" checksum="0CFFFAE2D9FFEF48" modified="2018-03-28" version="1">MIAIAGAVVAGAAAAAAVLVLRSSRDDDDDVRPEGAVAAVWPSQECAVCLSELVAGTTGGSCDSEVRMLAGCGHGFHEECIGRWLPLRPECPLCRCPVPVPEAEGESTEAAVVAVAPQAAAWSRPARIACGFGDGRVVWTRSPPTVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-06-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C3SA56</accession>
    <name>C3SA56_BRADI</name>
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        <submittedName>
            <fullName evidence="2 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104583827</name>
        <name evidence="3" type="ORF">BRADI_3g52890v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2009" name="Plant Mol. Biol." volume="70" first="47" last="61">
            <title>Structural characterization of Brachypodium genome and its syntenic relationship with rice and wheat.</title>
            <authorList>
                <person name="Huo N."/>
                <person name="Vogel J.P."/>
                <person name="Lazo G.R."/>
                <person name="You F.M."/>
                <person name="Ma Y."/>
                <person name="McMahon S."/>
                <person name="Dvorak J."/>
                <person name="Anderson O.D."/>
                <person name="Luo M.C."/>
                <person name="Gu Y.Q."/>
            </authorList>
            <dbReference type="PubMed" id="19184460"/>
            <dbReference type="DOI" id="10.1007/s11103-009-9456-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="EU730895">
        <property type="protein sequence ID" value="ACF22690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK00957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010237567.3"/>
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    <dbReference type="AlphaFoldDB" id="C3SA56"/>
    <dbReference type="EnsemblPlants" id="KQK00957">
        <property type="protein sequence ID" value="KQK00957"/>
        <property type="gene ID" value="BRADI_3g52890v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583827"/>
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        <property type="protein sequence ID" value="KQK00957"/>
        <property type="gene ID" value="BRADI_3g52890v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583827"/>
    <dbReference type="eggNOG" id="ENOG502S78J">
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        <property type="component" value="Chromosome 3"/>
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        <property type="entry name" value="OS02G0708000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ACF22690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK00957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="172" mass="18557" checksum="956C1D293E071747" modified="2009-06-16" version="1">MASLPQTMPYYPTSNAIMHAQPATSSRGSFGPVFTVLGVISFLAVAACIAGRLCGRRLSKKKSSSDQQFYSTNAVGGDLEKGFELKYPPMKPMPSSRAVIHDMDDGFEIKFAPGKPAAWKNDNKGDNRGRQQHPQVAMPKEYAGFRYPAAANGVVRQGQVRGGTFISAKPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-06-16" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>C3SAD7</accession>
    <name>C3SAD7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Myb-like DNA-binding domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100836777</name>
        <name evidence="11" type="ORF">BRADI_3g17165v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2009" name="Plant Mol. Biol." volume="70" first="47" last="61">
            <title>Structural characterization of Brachypodium genome and its syntenic relationship with rice and wheat.</title>
            <authorList>
                <person name="Huo N."/>
                <person name="Vogel J.P."/>
                <person name="Lazo G.R."/>
                <person name="You F.M."/>
                <person name="Ma Y."/>
                <person name="McMahon S."/>
                <person name="Dvorak J."/>
                <person name="Anderson O.D."/>
                <person name="Luo M.C."/>
                <person name="Gu Y.Q."/>
            </authorList>
            <dbReference type="PubMed" id="19184460"/>
            <dbReference type="DOI" id="10.1007/s11103-009-9456-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KQJ95429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014755642.1">
        <property type="nucleotide sequence ID" value="XM_014900156.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C3SAD7"/>
    <dbReference type="STRING" id="15368.C3SAD7"/>
    <dbReference type="EnsemblPlants" id="KQJ95429">
        <property type="protein sequence ID" value="KQJ95429"/>
        <property type="gene ID" value="BRADI_3g17165v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836777"/>
    <dbReference type="Gramene" id="KQJ95429">
        <property type="protein sequence ID" value="KQJ95429"/>
        <property type="gene ID" value="BRADI_3g17165v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836777"/>
    <dbReference type="eggNOG" id="KOG0048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028567_16_5_1"/>
    <dbReference type="OMA" id="MANGGGW"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000348">
        <property type="entry name" value="Transcription factor MYB26"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Myb_dom"/>
    </dbReference>
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        <property type="entry name" value="Plant_SW-associated_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997:SF31">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
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        <location>
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        <location>
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            <end position="116"/>
        </location>
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        </location>
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        </location>
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            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ACF22771.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ95429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="341" mass="38025" checksum="E9E70E0C00EA47E1" modified="2009-06-16" version="1">MGHHSCCNQQKVKRGLWSPEEDEKLVRYITTHGYGCWSEVPEKAGLQRCGKSCRLRWINYLRPDIRRGRFTPEEEKLIISLHAIVGNRWAHIASHLPGRTDNEIKNYWNSWIKKKIRKHVPAPPASISPTTASPPNSLIAAPPCTGSTGTSSVAGGANHRRPLQHPTFSCTADEDEQHHLQLDAFMGPQAQAQQSIVSCGRDEGSPPGGATMAAAPPPLFMFDTSGPLFATTPAQEHPFISSFTAAMAEGGCYGLQLPPLVDGMGAMAMDDHCRYEIVEEQKQQQQRRRPEPELELELEEERWVDDEQLLMWHDVHDELTTTPSSNLQSDHHSSLLFMGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNC0</accession>
    <name>I1GNC0_BRADI</name>
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        <recommendedName>
            <fullName evidence="3">SMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100827620</name>
        <name evidence="4" type="ORF">BRADI_1g08740v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LEA type SMP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312445.1">
        <property type="nucleotide sequence ID" value="XM_024456677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNC0"/>
    <dbReference type="STRING" id="15368.I1GNC0"/>
    <dbReference type="EnsemblPlants" id="KQK13229">
        <property type="protein sequence ID" value="KQK13229"/>
        <property type="gene ID" value="BRADI_1g08740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827620"/>
    <dbReference type="Gramene" id="KQK13229">
        <property type="protein sequence ID" value="KQK13229"/>
        <property type="gene ID" value="BRADI_1g08740v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R3IV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115598_0_0_1"/>
    <dbReference type="OMA" id="LTWEATM"/>
    <dbReference type="OrthoDB" id="680622at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042971">
        <property type="entry name" value="LEA_SMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007011">
        <property type="entry name" value="LEA_SMP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31174:SF4">
        <property type="entry name" value="OS03G0747500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31174">
        <property type="entry name" value="SEED MATURATION FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04927">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SMP" evidence="3">
        <location>
            <begin position="60"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="SMP" evidence="3">
        <location>
            <begin position="123"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK13229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="198" mass="20307" checksum="7211646FC77045DF" modified="2012-06-13" version="1">MAEARSRLDCRVYSEAEPSYQGREFAAEPVAPRDDAEFVAERLAHRGDDGVVDEGYDTKVKISEALEAAARAVGGDPVERSDAAAIRAAEARAVGAPIPGGVAEQAQAAADANAGAEREEDKVTIADVVAWNATAKLPTDKAVTEEDAAAVAEAEAAHGAPDGAAARGKPYGVGEALAAAARHNQEDVLVGKTITDCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQC3</accession>
    <name>I1GQC3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100832734</name>
        <name evidence="4" type="ORF">BRADI_1g14810v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562291.1">
        <property type="nucleotide sequence ID" value="XM_003562243.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQC3"/>
    <dbReference type="STRING" id="15368.I1GQC3"/>
    <dbReference type="EnsemblPlants" id="KQK14220">
        <property type="protein sequence ID" value="KQK14220"/>
        <property type="gene ID" value="BRADI_1g14810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832734"/>
    <dbReference type="Gramene" id="KQK14220">
        <property type="protein sequence ID" value="KQK14220"/>
        <property type="gene ID" value="BRADI_1g14810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832734"/>
    <dbReference type="eggNOG" id="ENOG502RXWM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085865_0_0_1"/>
    <dbReference type="OMA" id="GSAIHKQ"/>
    <dbReference type="OrthoDB" id="686179at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053266">
        <property type="entry name" value="Zinc_finger_protein_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47593:SF8">
        <property type="entry name" value="OS12G0581900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47593">
        <property type="entry name" value="ZINC FINGER PROTEIN 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK14220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="236" mass="25583" checksum="00FBB700C36F5F34" modified="2012-06-13" version="1">MRDRDMEQTDEASANKGSPVAGEQQGLEDGGPAAVTWLHLTLGVNSSQQPVAADSSNSSLDSEPPPARTTTPPSPAPSKPPPHKVFSCNFCMRKFFSSQALGGHQNAHKRERSAAKRSSSYHHHAHSSRQHQRMVMAGLPLEAHAAIMRAALRVNPVIHKQAPLATQDATAPRFHDGVGVVGPWAAPPLVYEDALRSTTTSWPGSFRMRTQSEPPSSEEHPPSDQQSKKMDLSLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRF9</accession>
    <name>I1GRF9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tyrosine-specific transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100846275</name>
        <name evidence="9" type="ORF">BRADI_1g18660v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752912.1">
        <property type="nucleotide sequence ID" value="XM_014897426.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRF9"/>
    <dbReference type="STRING" id="15368.I1GRF9"/>
    <dbReference type="EnsemblPlants" id="KQK14801">
        <property type="protein sequence ID" value="KQK14801"/>
        <property type="gene ID" value="BRADI_1g18660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846275"/>
    <dbReference type="Gramene" id="KQK14801">
        <property type="protein sequence ID" value="KQK14801"/>
        <property type="gene ID" value="BRADI_1g18660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846275"/>
    <dbReference type="eggNOG" id="ENOG502QSPD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038102_1_1_1"/>
    <dbReference type="OMA" id="PHIHQVN"/>
    <dbReference type="OrthoDB" id="204942at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GRF9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018227">
        <property type="entry name" value="Amino_acid_transport_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32195">
        <property type="entry name" value="OS07G0662800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32195:SF26">
        <property type="entry name" value="TRYPTOPHAN OR TYROSINE TRANSPORTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03222">
        <property type="entry name" value="Trp_Tyr_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK14801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="49872" checksum="BBD0F84AA30D2D5E" modified="2012-06-13" version="1">MAVASLQCYPLLNSPPLSVSRSISARTPAHRRAVGARWRLQRCCRSQSAESRSQDSQPQLERLFSNVNQATMKHEPGSVTGSIFLVAGTTVGAGILAIPAVTQEAGFLASAVTCIFCWIYMIVTGLLVAEVNVNTMCELGSGGVSLVSMAKRTLGTFGVRTACFSYLFIHYALLVAYVARSSDIITNALGIPLWESAALFSLAFGGLCYFGSQQVIGAVNGFLVFSIIASFTSLVVVASGNIQLSSLLETNFAAAPQSIPIIALSFVYQNVVPVLCTNLEGDLSKVRKAIVLGTAIPLVLFLVWDAVILGTIPGLAGSGTIADPLEQLRSSNGIVGPIVEAFSFLAIGTSYIGFVLGLSDFIADLLKLPSGQNKPLTYLVTLLPPLVLSLLDPEIFFKALDFAGTYGVLVLFGVFPAAMSWSERYSDELEAPVPPIVPGGKVTLSFVMGGALIVIFSEIFKDIMQLQGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRL3</accession>
    <name>I1GRL3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100829367</name>
        <name evidence="2" type="ORF">BRADI_1g19120v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562532.1">
        <property type="nucleotide sequence ID" value="XM_003562484.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRL3"/>
    <dbReference type="FunCoup" id="I1GRL3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK14867">
        <property type="protein sequence ID" value="KQK14867"/>
        <property type="gene ID" value="BRADI_1g19120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829367"/>
    <dbReference type="Gramene" id="KQK14867">
        <property type="protein sequence ID" value="KQK14867"/>
        <property type="gene ID" value="BRADI_1g19120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829367"/>
    <dbReference type="eggNOG" id="ENOG502QQ21">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051754_0_0_1"/>
    <dbReference type="OMA" id="FTWFGIT"/>
    <dbReference type="OrthoDB" id="419711at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242">
        <property type="entry name" value="OS02G0130600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242:SF6">
        <property type="entry name" value="PROTEIN ROLLING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK14867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="323" mass="36844" checksum="E529EAAC06B7ED8A" modified="2012-06-13" version="1">MSTPADGAAYWLRWQVFVCGALIALPTVAAAALLPRLRRSAAPLRATDLWVPCWSRLHPGWLLGYRAFALAAAAALLLRDVLAHGHGLFVFYFYTQWTFLLVTIYFAFATAISAHGCWAYSKKNLRKADESHGFLNGDVENRDLSSSISGERKKDEIDKMTSYYEQIANEKRAGFWGRCMQIIYQASAGATMLTDVTFWGLLVPFFYRDKFGLAMVTDGMHSLNAIFLLIDTILNNMPFPWYRMAFFVFWSCAYVTFQWVLHACGGLSWWPYPFLDLSSSGAPLWYLAMAIAHVPCFSLYWFIVKGKHTYFPRLFPHSYVRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZL0</accession>
    <name>I1GZL0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">shikimate kinase</fullName>
            <ecNumber evidence="6">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100836535</name>
        <name evidence="19" type="ORF">BRADI_1g45304v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the shikimate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="6"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564057.1">
        <property type="nucleotide sequence ID" value="XM_003564009.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZL0"/>
    <dbReference type="FunCoup" id="I1GZL0">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GZL0"/>
    <dbReference type="EnsemblPlants" id="KQK18894">
        <property type="protein sequence ID" value="KQK18894"/>
        <property type="gene ID" value="BRADI_1g45304v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836535"/>
    <dbReference type="Gramene" id="KQK18894">
        <property type="protein sequence ID" value="KQK18894"/>
        <property type="gene ID" value="BRADI_1g45304v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836535"/>
    <dbReference type="OrthoDB" id="197068at2759"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GZL0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000822">
        <property type="entry name" value="Shikimate kinase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21087">
        <property type="entry name" value="SHIKIMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21087:SF24">
        <property type="entry name" value="SHIKIMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="17" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQK18894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="302" mass="32083" checksum="725A2A9A1ABD382F" modified="2012-06-13" version="1">MEAGAGLAMQSSSRAVGVGARGGPGGCRSGALRGERRARLGSLRVGGLAAAAAAVRVRGAKPVAPLYCLKTSRGQETLHNSVDEALLLKRKAEEVLFELNGRCIYLVGMMGSGKSTVGKILAEVLGYSYFDSDSLVEQAVGMPSVAQIFKVHSEAFFRDNESSVLRDLSSMQRLVVATGGGAVIRPVNWRYMKKGLSIMLDVPLDALAKRIAQVGTASRPLLDQPSGDPYTAAFTKLSMLAEQRGDAYANADVRVSLEDIAAKQGHDDVSQLTPTDIAIEALQKIQNFVMEHSVASSPFDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1H125</accession>
    <name>I1H125_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g49390v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H125"/>
    <dbReference type="FunCoup" id="I1H125">
        <property type="interactions" value="323"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK19624">
        <property type="protein sequence ID" value="KQK19624"/>
        <property type="gene ID" value="BRADI_1g49390v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19624">
        <property type="protein sequence ID" value="KQK19624"/>
        <property type="gene ID" value="BRADI_1g49390v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R718">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1236542_0_0_1"/>
    <dbReference type="InParanoid" id="I1H125"/>
    <dbReference type="OMA" id="RYRECIS"/>
    <dbReference type="OrthoDB" id="629996at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36480:SF10">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN LEA-2 SUBGROUP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36480">
        <property type="entry name" value="OS06G0118900 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK19624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="24718" checksum="37CA176E441F26A1" modified="2012-06-13" version="1">MAADQSSGKTTTTCRRFRWLYVARWAVATAVTILAVAVIIRAVVVLLRPEKLQISLAEGRVAVDYIPSLPPPGNAVTFQFLLRASNPSGRASIVYTNVTIRLTDVSVPRAIAGFGLSPTVVAVSQRTTRETAVTVGLTPGDDVPMTYVRALFEGRSVGGVEMEVSGLLASHVAMAAKGGVTTTAVTTTSGAASYYCWPVTIAVVDAAQQATSDDYGGDGGGGGGHDTACLDKSEAPAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1H2H8</accession>
    <name>I1H2H8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g53600v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H2H8"/>
    <dbReference type="EnsemblPlants" id="KQK20291">
        <property type="protein sequence ID" value="KQK20291"/>
        <property type="gene ID" value="BRADI_1g53600v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20291">
        <property type="protein sequence ID" value="KQK20291"/>
        <property type="gene ID" value="BRADI_1g53600v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_37_8_1"/>
    <dbReference type="InParanoid" id="I1H2H8"/>
    <dbReference type="OMA" id="HEAIVWK"/>
    <dbReference type="OrthoDB" id="442680at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000931">
        <property type="entry name" value="Os07g0239600 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000305">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF494">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="76"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="209"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="313"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="414"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="629"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK20291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="721" mass="80320" checksum="00D22A1AA7C73CC7" modified="2012-06-13" version="1">MRYAARKAAAALQSPPTLSSTSSAPLLQRSQSLCASAPEKGLARISQEIVSLPISCVRGHHFHNARKLLDETPKRSASAWTAFIAGCARSGRHMDGLSAFTEMRAKGGAEPNAFVLAAVLRCCAGLRDMESGKCIHGWMLRNGAHLDVVLCNAVLDMYAKCGDYECTKRAFKAMTKIDAVSWNIVISACMQNGDILGSMQLFDVSPLRDTSSWNTVISGLMRNGCAAKALDCLYRMARARVVFNHYTYSTALALAGMLSLQDLGRQLHGRVLTAAVDTDAFVRSSLMDMYCKCGSMETAVLIFDSWSQLTGDMNFAWSAMVAGYVQNGREEEAFEFFRSMLREGVAAHQFTLTSAIAACANAGMVEQGRQVHGCIEKLGHSFDAPLASAIIDMYAKCGNLGDACRIFDRACAKNVALWTSMLCSYASHGKGRMAIELFNRMTAEKITPNEITLVGVLSACSHGRLVNEGDHFFKLMQDEYGIVPSIEHYNCMVDLYGRAGMLYKAHNFIKENNIKHEAIVWKTLLSACRLHKHMEYAKLASENLIQLEECDAGSYVMLSNVYATHSKWLDTSKLRSSMRERRVWKQPGRSWIHLKNIMHTFVAGDTAHPRSAEIYAYLEKLMERLKDLGYTSRTDLVAHDVEEEQRETALKFHSEKLAIAFGIISTPSGTPLRIFKNLRVCVDCHEAIKYVSQATDREIVVRDLYRFHHFKDAKCSCEDFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4W6</accession>
    <name>I1H4W6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100824728</name>
        <name evidence="1" type="ORF">BRADI_1g60770v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557790.1">
        <property type="nucleotide sequence ID" value="XM_003557742.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H4W6"/>
    <dbReference type="STRING" id="15368.I1H4W6"/>
    <dbReference type="EnsemblPlants" id="KQK21441">
        <property type="protein sequence ID" value="KQK21441"/>
        <property type="gene ID" value="BRADI_1g60770v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824728"/>
    <dbReference type="Gramene" id="KQK21441">
        <property type="protein sequence ID" value="KQK21441"/>
        <property type="gene ID" value="BRADI_1g60770v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824728"/>
    <dbReference type="eggNOG" id="ENOG502RXUK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138191_0_0_1"/>
    <dbReference type="OMA" id="CLPFHAV"/>
    <dbReference type="OrthoDB" id="20282at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H4W6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030482">
        <property type="entry name" value="PDRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21162">
        <property type="entry name" value="P53 AND DNA DAMAGE-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21162:SF3">
        <property type="entry name" value="P53 AND DNA DAMAGE-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK21441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="166" mass="18871" checksum="215BCFB39E547195" modified="2012-06-13" version="1">MDPNMKHLQETLVDIETDAEQLLLARHQLVENDKMRNANREALTALRKKARTTKTSVPSPFDVIMKEMEGTSGRELIKEICPTCGNHDPKEHTWLMFPGSDIFARVPFHVTHTILDKDQERLDYDSKKLQSFVKEKSFLISEKGALADKISPGIVKSLVSLTDKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1H787</accession>
    <name>I1H787_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phospholipase A2</fullName>
            <ecNumber evidence="5">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100827006</name>
        <name evidence="16" type="ORF">BRADI_1g67460v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Releases lysophospholipids (LPLs) and free fatty acids (FFAs) from membrane phospholipids in response to hormones and other external stimuli.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phospholipase A2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="5"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558308.1">
        <property type="nucleotide sequence ID" value="XM_003558260.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H787"/>
    <dbReference type="EnsemblPlants" id="KQK22477">
        <property type="protein sequence ID" value="KQK22477"/>
        <property type="gene ID" value="BRADI_1g67460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827006"/>
    <dbReference type="Gramene" id="KQK22477">
        <property type="protein sequence ID" value="KQK22477"/>
        <property type="gene ID" value="BRADI_1g67460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827006"/>
    <dbReference type="eggNOG" id="ENOG502RYYJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115623_0_0_1"/>
    <dbReference type="OMA" id="CVDTHNN"/>
    <dbReference type="OrthoDB" id="1932081at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04706">
        <property type="entry name" value="PLA2_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000005">
        <property type="entry name" value="Secretory phospholipase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094363" description="phospholipase A2" evidence="15">
        <location>
            <begin position="27"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK22477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="155" mass="16355" checksum="E64E0293BBB84651" modified="2012-06-13" version="1">MASFLVASPRCSLLLLLLLLVTASRGLKTGDLVSQGKASADCSRTCESKYCTVAPVMRYGKYCGILYSGCPGEKPCDALDACCMVHDHCVDANNNDYLSTKCNENLLSCLDGVSTAGPTFPGNKCGVGEVAFVIKGVIETAVLAGKILHRRDIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9S4</accession>
    <name>I1H9S4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tonoplast intrinsic protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100828833</name>
        <name evidence="11" type="ORF">BRADI_1g75290v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aquaporins facilitate the transport of water and small neutral solutes across cell membranes. May be involved in transport from the vacuolar compartment to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC 1.A.8.10) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558815.1">
        <property type="nucleotide sequence ID" value="XM_003558767.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H9S4"/>
    <dbReference type="SMR" id="I1H9S4"/>
    <dbReference type="FunCoup" id="I1H9S4">
        <property type="interactions" value="556"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H9S4"/>
    <dbReference type="EnsemblPlants" id="KQK23668">
        <property type="protein sequence ID" value="KQK23668"/>
        <property type="gene ID" value="BRADI_1g75290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828833"/>
    <dbReference type="Gramene" id="KQK23668">
        <property type="protein sequence ID" value="KQK23668"/>
        <property type="gene ID" value="BRADI_1g75290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828833"/>
    <dbReference type="eggNOG" id="KOG0223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_3_4_1"/>
    <dbReference type="OMA" id="GPFHWIF"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006833">
        <property type="term" value="P:water transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000002">
        <property type="entry name" value="Probable aquaporin TIP1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF54">
        <property type="entry name" value="AQUAPORIN TIP1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK23668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="25717" checksum="D6145BBDE30E4F7C" modified="2012-06-13" version="1">MPISRVAVGSHHEVYQAGALKAAVAEFISTLIFVFAGQGSGMAFNKLNAGGAATPAGLISAAIAHAFALFVAVSVGANISGGHVNPAVTFGAFLGGNITLFRGLLYWVAQLLGSTAACFLLRFSTGGLPTGTFGLTGVSVWEALVLEIVMTFGLVYTVYATAVDPKKGSIGTIAPLAIGFIVGANILVGGAFDGASMNPAVSFGPALVSWEWGYQWVYWVGPLIGGGLAGVIYEVLFISHTHEQLPTTDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1HA70</accession>
    <name>I1HA70_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100832077</name>
        <name evidence="20" type="ORF">BRADI_1g76560v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">Bd21</strain>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229263.1">
        <property type="nucleotide sequence ID" value="XM_010230961.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312443.1">
        <property type="nucleotide sequence ID" value="XM_024456675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HA70"/>
    <dbReference type="STRING" id="15368.I1HA70"/>
    <dbReference type="EnsemblPlants" id="KQK23854">
        <property type="protein sequence ID" value="KQK23854"/>
        <property type="gene ID" value="BRADI_1g76560v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK23856">
        <property type="protein sequence ID" value="KQK23856"/>
        <property type="gene ID" value="BRADI_1g76560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832077"/>
    <dbReference type="Gramene" id="KQK23854">
        <property type="protein sequence ID" value="KQK23854"/>
        <property type="gene ID" value="BRADI_1g76560v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK23856">
        <property type="protein sequence ID" value="KQK23856"/>
        <property type="gene ID" value="BRADI_1g76560v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_37_1_1"/>
    <dbReference type="OMA" id="DAPQCQA"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HA70">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009931">
        <property type="term" value="F:calcium-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000249">
        <property type="entry name" value="Calcium-dependent protein kinase SK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="80"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="382"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="454"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="488"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KQK23856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="550" mass="61512" checksum="8408502E958C0347" modified="2012-06-13" version="1">MGNQNGTLGTDCYHNRYPRARPVSVYSDGYVEGGGYLDLKKPLPEANSLKPSAAGILRQGLDPTSISVLGRKTADLRQNYILGRKLGQGQFGTTYLCTEISTGCEYACKTILKRKLITKVDVEDVRREIQIMHHLSGHKNVVSIKDVYEDGQAVHIVMELLAGGELFDRIKGKGYYSELKAAEIIKIVISIVAMCHSLGVMHRDLKPENFLLLDKDDDLSIKAIDFGLSIFFKPGQVFSELVGSPYYLAPEVLNKRYGPESDVWSAGVILYVLLSGVPPFWSAETPQGIFDAVLKGHIDFESEPWPKISDSAKDLIRKMLCHCPSERLKAHEVLRHPWICKNGVTTGQALDPSIISQLNEFSAMKNLKKLALRVIAERLSEEEIAGLREMFKAVDINNRGVITFGELRKGLTRYSNELEDAKISDIMEMADRDDNVTINYEEFIAATMPRNKIECEEHLMAAFTYFDKDGSGYITIDKLQRAFGDHNMEVTFLEEIILEVDQNNDGQIDYAEFVAMMQGNNSTGDGCQKLETNSNVTLRDAPQLDGPKVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAY2</accession>
    <name>I1HAY2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin B-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100821249</name>
        <name evidence="6" type="ORF">BRADI_1g78640v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Interacts with CIPK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559049.1">
        <property type="nucleotide sequence ID" value="XM_003559001.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAY2"/>
    <dbReference type="STRING" id="15368.I1HAY2"/>
    <dbReference type="EnsemblPlants" id="KQK24188">
        <property type="protein sequence ID" value="KQK24188"/>
        <property type="gene ID" value="BRADI_1g78640v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821249"/>
    <dbReference type="Gramene" id="KQK24188">
        <property type="protein sequence ID" value="KQK24188"/>
        <property type="gene ID" value="BRADI_1g78640v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821249"/>
    <dbReference type="eggNOG" id="KOG0034">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_21_0_1"/>
    <dbReference type="OMA" id="MFQQAWI"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HAY2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000073">
        <property type="entry name" value="calcineurin B-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045198">
        <property type="entry name" value="CNBL1-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056">
        <property type="entry name" value="CALCINEURIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056:SF103">
        <property type="entry name" value="CALCINEURIN B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="147"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK24188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="212" mass="24413" checksum="820BCD854449287F" modified="2012-06-13" version="1">MGCFHSKPRRQYPGYEDPISLASQTTFSVSEVEALFELFKSISGSVIDDGLINKEEFQLALFKSKKDNLFANRIFDLFDVKKRGVIDFADFVRALNVFHPSIPMEEKIDFSFKLYDMDNTGFIERNEVKQMLVALLSESEMRLSDETIETILDKTFSDADVNQDGKIDRAEWENFVSRNPSLLKIMTLPYLKDITTTFPSFVFHSEVDDIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HF38</accession>
    <name>I1HF38_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100839593</name>
        <name evidence="5" type="ORF">BRADI_2g12347v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230853.1">
        <property type="nucleotide sequence ID" value="XM_010232551.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HF38"/>
    <dbReference type="EnsemblPlants" id="KQK04209">
        <property type="protein sequence ID" value="KQK04209"/>
        <property type="gene ID" value="BRADI_2g12347v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839593"/>
    <dbReference type="Gramene" id="KQK04209">
        <property type="protein sequence ID" value="KQK04209"/>
        <property type="gene ID" value="BRADI_2g12347v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839593"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001724_0_1_1"/>
    <dbReference type="OMA" id="FDIYYNY"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000135">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1344">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK04209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="495" mass="53452" checksum="E7061ED972F39D8C" modified="2012-06-13" version="1">MESRRRENPPAPQPHLLFVTSPLQGHINPVRRLAARVAGAALVTVSTAVSGHRRMFPSLASPDEEAIEGNGMLHAPYSDGFDEGFDPEIHDVRSYGPRARAVGCETLSGVVARLARRGRPVTRVVYTFLVPWAPDVARAHGVPAALFWIQPAAVFAVYYHFFHGHEAVLASCADDEDGIVSLPGLPPLRPRALPSIVLTTAPEQQRHTVLQTLRELFLALDDDEQQQQQQHRPKVLVNTFDALEPEALRAVPQFELVAVGPVVPPEPDDASSPSSTDLSLFGGHDVEKQASMEEWLGTKAARSVVYVSFGSLIAASKRQEAELRRGLEATGRPYLWVSSTAAAADEEFPDTELLEGTNNGMVVDWCDQARVLSQPAVGCFVTHCGWNSALESVACGVPVVAVPQWTDQPTVAWIVEECAGVGVRARVDGEGVAEGGEIRRCVEAVMGNVDDVAVGIRANASRWRERAMEAIASAGTLDKNLRAFVSGLPRNALDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJV4</accession>
    <name>I1HJV4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC104582676</name>
        <name evidence="5" type="ORF">BRADI_2g26640v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the fantastic four family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231449.1">
        <property type="nucleotide sequence ID" value="XM_010233147.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJV4"/>
    <dbReference type="STRING" id="15368.I1HJV4"/>
    <dbReference type="EnsemblPlants" id="KQK06493">
        <property type="protein sequence ID" value="KQK06493"/>
        <property type="gene ID" value="BRADI_2g26640v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104582676"/>
    <dbReference type="Gramene" id="KQK06493">
        <property type="protein sequence ID" value="KQK06493"/>
        <property type="gene ID" value="BRADI_2g26640v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104582676"/>
    <dbReference type="eggNOG" id="ENOG502RYS2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077801_0_0_1"/>
    <dbReference type="OMA" id="PRMRMVH"/>
    <dbReference type="OrthoDB" id="1916983at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021410">
        <property type="entry name" value="FAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046431">
        <property type="entry name" value="FAF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33155">
        <property type="entry name" value="FANTASTIC FOUR-LIKE PROTEIN (DUF3049)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33155:SF8">
        <property type="entry name" value="PROTEIN FANTASTIC FOUR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11250">
        <property type="entry name" value="FAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAF" evidence="4">
        <location>
            <begin position="107"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK06493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="236" mass="25922" checksum="EC6A93B817E7C3A1" modified="2012-06-13" version="1">MTNSTLYSPPQIPAAGPWSSLYAPQEQPAKPRDASAVAVLPSSSPKKPAYGVKRNLEMCTEALGCETGGFDAAGDEEADEGAERKRRARREETKVEATRVRRRVLPLPPPLTTLAAGVARTRMVHERRDGRLEVYAVRAAGMEAQRSGGRLQLRFRPCHGCNIGAARSIQRETEDQAEEEAEEVDQQREQEEEYGVVAKYVRGGRCAEMAEGAATAARRGEKWEPEQAAAFWVAIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HLA8</accession>
    <name>I1HLA8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bicarbonate transporter-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100843873</name>
        <name evidence="10" type="ORF">BRADI_2g34110v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anion exchanger (TC 2.A.31.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568821.1">
        <property type="nucleotide sequence ID" value="XM_003568773.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231659.1">
        <property type="nucleotide sequence ID" value="XM_010233357.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HLA8"/>
    <dbReference type="STRING" id="15368.I1HLA8"/>
    <dbReference type="EnsemblPlants" id="KQK07242">
        <property type="protein sequence ID" value="KQK07242"/>
        <property type="gene ID" value="BRADI_2g34110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843873"/>
    <dbReference type="Gramene" id="KQK07242">
        <property type="protein sequence ID" value="KQK07242"/>
        <property type="gene ID" value="BRADI_2g34110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843873"/>
    <dbReference type="eggNOG" id="KOG1172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002289_3_2_1"/>
    <dbReference type="OMA" id="QFWERTQ"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000004">
        <property type="entry name" value="probable boron transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF31">
        <property type="entry name" value="BORON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0039">Anion exchange</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="547"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="9">
        <location>
            <begin position="7"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="9">
        <location>
            <begin position="202"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="9">
        <location>
            <begin position="454"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK07242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="678" mass="76620" checksum="8C245A19A4699F4F" modified="2012-06-13" version="1">MEHKKTLFKGVIEDFRGRAACYKQDWHNGFSSGFRILAPTLYIFFASALPVIAFGEQLSKDTDGTLTTVETLASTAICGIIHSVMGGQPLLIVGVAEPTIIMYTYIYNFAKNQPSLGERLFLAWAGWVCVWTAVMLFLMAMFNAAVALNRFTRFAGELFGMLITILFMQEAVKGMLSEFSAPEGQDHNLPIYQFHWVYINGLLGIIFSMGLLYTALRSRAARSSLYGTGWQRSLIADYGVPIMVILWTALSYSLPSKIPSGVPRRLFTPLPWEPKSLQHWTVAKDLFSVPPAYIFLAIVPAAMVAGLYFFDHSVASQMAQQKEFNLKNPSAYHYDILVLSFSVLICGLLGIPPSNGVLPQSPMHTRSLAVLRRQALRKKMIQTAKEGMMNNANCSEVYGKMQEVFIEMDDKSNEVSVHKELRDLKDAVIPEGNGAGNVPEVFDPEKHLEAYLPVRVNEQRVSNLLQSLLVAGCVGVMPIIQKIPTSVLWGYFAYMSIDSLPGNQFWERFQLLFVAPQRRYKVLEGAHASFLESVPFNKIFAFTLFQLFYLLIVWGMTWIPVAGILFPLLFFFLVLIRQHFLPKFFDSRHLWELDASEYEECEGVRRDPSISEADNESIAHGGEAPPEYAHDILEEFTTHRGELKHRAPSFRDERLIRLNSVQMTRQFSRIASLAPERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HLT0</accession>
    <name>I1HLT0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100832236</name>
        <name evidence="11" type="ORF">BRADI_2g35767v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231698.1">
        <property type="nucleotide sequence ID" value="XM_010233396.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231699.1">
        <property type="nucleotide sequence ID" value="XM_010233397.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315711.1">
        <property type="nucleotide sequence ID" value="XM_024459943.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315712.1">
        <property type="nucleotide sequence ID" value="XM_024459944.1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HLT0"/>
    <dbReference type="EnsemblPlants" id="KQK07493">
        <property type="protein sequence ID" value="KQK07493"/>
        <property type="gene ID" value="BRADI_2g35767v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832236"/>
    <dbReference type="Gramene" id="KQK07493">
        <property type="protein sequence ID" value="KQK07493"/>
        <property type="gene ID" value="BRADI_2g35767v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832236"/>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000837_8_4_1"/>
    <dbReference type="OMA" id="AIRGAQW"/>
    <dbReference type="OrthoDB" id="685117at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HLT0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF30">
        <property type="entry name" value="TIR-NBS TYPE DISEASE RESISTANCE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="293"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="545"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="10">
        <location>
            <begin position="815"/>
            <end position="929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK07493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1484" mass="167122" checksum="9ECA7E284009628C" modified="2012-06-13" version="1">MEPAAITGAQWVVGKALSPLSDGLVEAWAATSELGPNIEALKMELLYAQAMLENARGREIPSQALGELLQRLRGLAYGADDVLDELDYFRIQDELEGTFEAAVDDDGRGCFHNLVRDGRHTAKAAAKQLGCCSCSALIHDYKPEDPCKCVRRLASRAHAVGKRFLCSSPQLVRCDGRGDDNRHAPRVPKLKFDRVDVSRKMKCIVEQLKPVCSKVSSILDLELLGSAVAKLELLGSAVAKLESLGSNRAMGNVASSTTSRSITTSQALEPKLYGREPEKKTIVEDITRGAYIHRDLTVVPIVGPGGIGKTTLTQDIYNSQEVQDHFQIRVWICVSLDFSVYRLTQEILSSIPKAEDEKNERTDDAIKNLDQLQKLVQRRLKNKRFLLVLDDIWSYGNEDEWKRFLVPFTEEQGKGNIVLATTRFLHVAEIVKKGDKSLPLEGLGPKDYWRLFLACVFDERNQQCNDGELLQIGKMIVEKLKGSPLAAKTVGRLLRKNLTVDHWTRVLESKEWESQTSDHDIMPALKLSYDFLPFQLQQCFSCWALFPEDYKFDCEELIHFWIGLDILRPSHTTKRIEEIGRNNLNELVSYGFFIEVTGKSDKQYVMHDLLHDLALKVSSQECLHLASSSPRPVEIAPSIYHLSISLSPANSGDGIMDEKFKKELGKIKNILKSENLHTLMLFGDYDANFLRIFSDLFKDAKKLRVVHLSTMYYSVESLLHNFSKLIHLRYLRLVPQFGSKEHLPSSISRFYQLRVLDIRRGSHSSLRDMSNLVKLRHFLSYNVEHHSNISNVGKLDSLQELQRFEVRKESNGFELRELGHLEELGGSLGIYNLENVQASEAHEAKLMYKRHLQKLTLSWNKGRSNTNPDVEDQILESLRPHSNLHEVCIDGHGGVTCPTWLGTNLFTKGLEALRLDGVAWKSLPPLGEMWLIDESGEEYFGCIRGLNFDNLRRLELIGLPRFRKWVANEVCPWYFSLIEELTVEDCPELTELPFSNSNCYSSEGDVNGTCFPRLTTLKIWNCEKLLSLPPLPYGHTLCSVSLRRAGRDLKRLSYSNTNLSSFLTIEGNDDLPKLDETVLAFQNLTQLQELYIADCPPLAEKHLQMLTSLKTLEIDVSRILFLPLARSDVKWQVPVNTLMISNSEASGKELTRLLSRFPELFNLEIRDCEKITRLGIEVEQQKQIAEDVVTEDAVAEQDEEEDGLLLLGPRLTGSLQELWISSCSKLVLTSGGGGLQAMCSLTEITIQDCPKFLSAYKTSSLCSSRPFPSSLQRLWLVGPMEGMETLAPLSNLTSLEELSLANLGEDLRCEGLWPLLAQGPLKELYVWSPNFFAGWDPAWGATSQEEQPLLSPSSKLQELSTDDIAGVLAAPICRLLSSSLTKLSIVSNKVTERFTKEQENALSLLSSLQELELKSCDKLRCLPAGLNKLTNLHRLQIWSCPAIRSLPKNGLPSSLQELDVSGCKNNELTQRCRRLKETIPNIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>I1HML2</accession>
    <name>I1HML2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Isovaleryl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.3.8.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100833691</name>
        <name evidence="20" type="ORF">BRADI_2g38100v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = 3-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:12276"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57345"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15 16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.4" evidence="5"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569046.1">
        <property type="nucleotide sequence ID" value="XM_003568998.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HML2"/>
    <dbReference type="FunCoup" id="I1HML2">
        <property type="interactions" value="2427"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HML2"/>
    <dbReference type="EnsemblPlants" id="KQK07864">
        <property type="protein sequence ID" value="KQK07864"/>
        <property type="gene ID" value="BRADI_2g38100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833691"/>
    <dbReference type="Gramene" id="KQK07864">
        <property type="protein sequence ID" value="KQK07864"/>
        <property type="gene ID" value="BRADI_2g38100v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833691"/>
    <dbReference type="eggNOG" id="KOG0141">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_1_1"/>
    <dbReference type="OMA" id="CFITNSG"/>
    <dbReference type="OrthoDB" id="9988775at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HML2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="term" value="P:L-leucine catabolic process"/>
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        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
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        <location>
            <begin position="35"/>
            <end position="148"/>
        </location>
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            <end position="247"/>
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            <begin position="259"/>
            <end position="407"/>
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    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="272"/>
        </location>
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        <location>
            <begin position="153"/>
            <end position="162"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
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            <end position="188"/>
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        </ligand>
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            <end position="209"/>
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        <source>
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    <sequence length="411" mass="44713" checksum="8AA7AB8686428173" modified="2012-06-13" version="1">MQRWLPALLRRAAGAGGGSPARRSLASCSSLLFDDTQEQFKESVHRFAQEHIAPHAAAIDASNYFPKEVNLWKLMGDFNLHGLTSPEEYGGLGLGYMYHCIAMEEISRASGSVGLSYGAHSNLCINQLVRNGSPAQKEKYLPKLISGEHVGALAMSEPNSGSDVVSMKCKAEKVDGGYVINGNKMWCTNGPSAQTLVVYAKTDITAGSKGITAFIIEKGMPGFSTAQKLDKLGMRGSDTCELVFENCFVPHENVLGEEGKGVYVMMSGLDLERLVLAAGPIGLMQACLDVVLPYVRQREQFGRPIGEFQFVQGKMADMYTSLQSSRSFVYSVARDCDNGKVDRKDCAGVILFAAERATQVALEAIQCLGGNGYINEYPTGRLLRDAKLFEIGAGTSEIRRMIIGRELFKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HRC4</accession>
    <name>I1HRC4_BRADI</name>
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        <recommendedName>
            <fullName evidence="12">Haloacid dehalogenase-like hydrolase family protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="10" type="primary">LOC100846410</name>
        <name evidence="9" type="ORF">BRADI_2g49310v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK09643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003569711.4"/>
    </dbReference>
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        <property type="interactions" value="1511"/>
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        <property type="protein sequence ID" value="KQK09643"/>
        <property type="gene ID" value="BRADI_2g49310v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846410"/>
    <dbReference type="Gramene" id="KQK09643">
        <property type="protein sequence ID" value="KQK09643"/>
        <property type="gene ID" value="BRADI_2g49310v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846410"/>
    <dbReference type="eggNOG" id="ENOG502QUN8">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_024900_1_0_1"/>
    <dbReference type="OMA" id="KMRKRAP"/>
    <dbReference type="OrthoDB" id="27226at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metalloendopeptidase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:rRNA processing"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="HAD_Pase"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
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        <property type="entry name" value="HAD_sf"/>
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        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
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        <property type="entry name" value="YbeY"/>
    </dbReference>
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        <property type="entry name" value="Cof-subfamily"/>
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    </dbReference>
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        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="rRNA maturation RNase YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986">
        <property type="entry name" value="ENDORIBONUCLEASE YBEY, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986:SF1">
        <property type="entry name" value="ENDORIBONUCLEASE YBEY, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK09643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="64367" checksum="DFA54FF548A55CE1" modified="2012-06-13" version="1">MARILSRALPFASHSHLQFSPPIRGAALLSSAPLLPHLPAASTVSLLSWRGFTPTPEPSRSVPPFAGFLAGIRGFRRARRGQAAAKREQAQEDPAPPPPPKESEIELYARIGVDEDLPDDLEVLNIIEILKLNVPMAMKIALDGLLEYNYNTRDTSISDVGKYEKVEVSVLLGNDNFIQNLNKEWRGEDCATDMLSMSQYIPDLDVPILMLGDIAISVETAARQAEERGHTLLDELRTLVVRGLLHLLGFDRQASNEAAVEMEKEELLILKSLRWKGKGFAKSALDLSKPRTETSDGRATNGLKKAGSLRFYRPKFKYIFCDLDGTLLNSKSRVTARNAEALKEARSRGVNIVIATGKTRPAAIDVLNMVGLSGRNGFVSESSPGVFLQGLVVYGLEGQEIYKRNLDQEVCREALLYSLEHKVPLAAFSKDRSFSLFEDPSVDSLHYVYHEPKAEIVSSIDQLLGTAEIQKVLFLGTPEGISTTLRPYWEKAIGERAGVVQGQPDMLELVPPATSKGRGVKMLLDHLCISPDEVMAIGDGENDIEMLQLASLGVAMANGAEKTKAVANVIGATNDEDGVAQAIYDYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUX4</accession>
    <name>I1HUX4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100831123</name>
        <name evidence="6" type="ORF">BRADI_2g59880v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567437.1">
        <property type="nucleotide sequence ID" value="XM_003567389.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUX4"/>
    <dbReference type="STRING" id="15368.I1HUX4"/>
    <dbReference type="EnsemblPlants" id="KQK11384">
        <property type="protein sequence ID" value="KQK11384"/>
        <property type="gene ID" value="BRADI_2g59880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831123"/>
    <dbReference type="Gramene" id="KQK11384">
        <property type="protein sequence ID" value="KQK11384"/>
        <property type="gene ID" value="BRADI_2g59880v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831123"/>
    <dbReference type="eggNOG" id="KOG2111">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025895_2_1_1"/>
    <dbReference type="OMA" id="FACGTEQ"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001083">
        <property type="entry name" value="Autophagy-related protein 18a isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK11384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="446" mass="48492" checksum="88E84915F7CEB39C" modified="2012-06-13" version="1">MATPNPSSFLDDPLPPEPAVREPKVREPEVRDPEPLEPESPLPAASIEPTPSGEDDSDDSSSVSSVTSTPAAAAGAGEGGGIERPFPAAKDLLHISFNQDYGCFAAGTKTGFRIYNCDPFREIFRRDLGGEDDAGGQGGGIGVVEMLFRCNILALVGGGNAPHYPPNKVMIWDDHQSRCIGELSFKSPVRGVRLRRDRIVVVLESKIFVYNFADLKLVHQIETAPNPKGLCAVSQQPGSIVLVCPGAQKGQVRVEHYGARKTKFINAHTSRVACFALSQDGRLIATASTKGTLVRIFNAAEGNLLQEVRRGADRAEIYSLAFSNNLQYLAVSSDKGTIHVFNLKINVGLTTNDMPLPAPDPDVPHMSPSFSFIKGVLPKYFHSEWSVAQFRLHEGEQYIVAFGHEKNTVAVVGMDGSFYRCQFDPVNGGEMQQLECHNFLKPSDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HX27</accession>
    <name>I1HX27_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Terpene cyclase/mutase family member</fullName>
            <ecNumber evidence="4">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100824616</name>
        <name evidence="7" type="ORF">BRADI_3g03490v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the terpene cyclase/mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574426.1">
        <property type="nucleotide sequence ID" value="XM_003574378.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HX27"/>
    <dbReference type="FunCoup" id="I1HX27">
        <property type="interactions" value="1428"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HX27"/>
    <dbReference type="EnsemblPlants" id="KQJ93257">
        <property type="protein sequence ID" value="KQJ93257"/>
        <property type="gene ID" value="BRADI_3g03490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824616"/>
    <dbReference type="Gramene" id="KQJ93257">
        <property type="protein sequence ID" value="KQJ93257"/>
        <property type="gene ID" value="BRADI_3g03490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824616"/>
    <dbReference type="eggNOG" id="KOG0497">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009074_2_0_1"/>
    <dbReference type="OMA" id="CWARQTI"/>
    <dbReference type="OrthoDB" id="602388at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016871">
        <property type="term" value="F:cycloartenol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02892">
        <property type="entry name" value="SQCY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000002">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000022">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002365">
        <property type="entry name" value="Terpene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01787">
        <property type="entry name" value="squalene_cyclas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764:SF20">
        <property type="entry name" value="LANOSTEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="TERPENE CYCLASE/MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13249">
        <property type="entry name" value="SQHop_cyclase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01016">
        <property type="entry name" value="Prenyltransferase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01074">
        <property type="entry name" value="TERPENE_SYNTHASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Squalene cyclase N-terminal" evidence="6">
        <location>
            <begin position="99"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene cyclase C-terminal" evidence="5">
        <location>
            <begin position="414"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ93257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="760" mass="86289" checksum="76E01A07AFDB8CF7" modified="2012-06-13" version="1">MWRLKVGEGGGDPWLRSKNAHVGRQVWEFDPRADDPDALAAVDAARRDFAAGRLRHKHSADRLMRIQFAKENPLKLDLPAIKLEEHEDVTEEAVSTSLKRAISRFSTLQAHDGHWPGDYGGPMFLMPGLLITLYVTGSLNTVLSSEHQKEICRYLYNHQNEDGGWGLHIEGPSTMFGSALTYVCLRLLGEGPDSGDGAMEKGRNWILDHGGATYITSWGKFWLAVLGVFDWSGNNPLPPEIWMLPYCLPIHPGRMWCHCRMVYLPMCYVYGKRFVGRITPIILELRKELFKVPYSEIDWDKARNLCAKEDLYYPHPLIQDILWATLHKFVEPVLTHWPGNKLREKALNLVMQHVHYEDENTRYICIGPVNKVLNMLTCWIEDPNSEAFKLHIPRIYDYLWVAEDGMKMQGYNGSQLWDTAFTVQAIAATGLIEEFAPTLKLAHDYIKNSQVIDDCPGDLDYWYRHISKGAWPFSTADHGWPISDCTAEGLKASLVLSKISPEFVGEPVEVNRLYDAVNCLMSWMNENGGFATYELTRSYAWLELINPAETFGDIVIDYPYVECTSAAIQALTSFKKLYPGHCRKDVDNCINKAANYIESIQRSDGSWYGSWAVCFTYGIWFGVKGLVAAGRTFKNSPAIRKACDFLKSKELPFGGWGESYLSSQDQVYTNLEGKHAHAVNTGWAMLALIDAGQAERDPMPLHRAAKVLINLQSEDGEFPQQEIMGVFNKNCMISYSQYRNIFPIWALGEYRCRVLAADKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXG0</accession>
    <name>I1HXG0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100840752</name>
        <name evidence="3" type="ORF">BRADI_3g04560v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574897.2">
        <property type="nucleotide sequence ID" value="XM_003574849.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXG0"/>
    <dbReference type="FunCoup" id="I1HXG0">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HXG0"/>
    <dbReference type="EnsemblPlants" id="KQJ93432">
        <property type="protein sequence ID" value="KQJ93432"/>
        <property type="gene ID" value="BRADI_3g04560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840752"/>
    <dbReference type="Gramene" id="KQJ93432">
        <property type="protein sequence ID" value="KQJ93432"/>
        <property type="gene ID" value="BRADI_3g04560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840752"/>
    <dbReference type="eggNOG" id="ENOG502QTMV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026333_0_0_1"/>
    <dbReference type="OMA" id="QYWHEPH"/>
    <dbReference type="OrthoDB" id="673865at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF65">
        <property type="entry name" value="FBD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="17"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="At1g61320/AtMIF1 LRR" evidence="2">
        <location>
            <begin position="161"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ93432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="480" mass="53379" checksum="6245C9872035886B" modified="2012-06-13" version="1">MVMKRERRCVGHGEDRISELPDALRLQILSLLPLKSAIRTGALSSKWRDLWEQRWPVPSSLRLRFPPSAAADQLAAFDRRGRRRADVFSMVFQTGQLAQPDLRRFLDYAAACCVEDLQLRLDGTGGRGSRGGQRRAGALAVHFPVGSPLLARLSVRGLHLTASANAMVATLEVIHLHSVSLTDAALRRVVAACPCLRELELRCCRHLRRIDFTTVGVPNLRSLTIVDCSRATELRVPAAPRLRSFRFSGPFLGSNLFSGTTDCFQHLYLCSGGPETGLQHTNLPSAIPHLANLTALTLCSMALQNLSASVASVGKESSLPKLRELQLLMFGMANSNLADIFSFLKTCRCPQLERLFVQLPTNTHDAFTTNFLEVAEEEPPEGGLENLRLVKMTNFKAHKNERRLVDFLLRKASCLNKLFLVAPKEDHPRGLQKVQSDVLSHFLETETPLLERASANTQIVFNEHDSPRVQPLHSEVFIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYU7</accession>
    <name>I1HYU7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100845194</name>
        <name evidence="6" type="ORF">BRADI_3g08356v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571126.1">
        <property type="nucleotide sequence ID" value="XM_003571078.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYU7"/>
    <dbReference type="STRING" id="15368.I1HYU7"/>
    <dbReference type="EnsemblPlants" id="PNT66194">
        <property type="protein sequence ID" value="PNT66194"/>
        <property type="gene ID" value="BRADI_3g08356v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845194"/>
    <dbReference type="Gramene" id="PNT66194">
        <property type="protein sequence ID" value="PNT66194"/>
        <property type="gene ID" value="BRADI_3g08356v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845194"/>
    <dbReference type="eggNOG" id="KOG0158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_9_1"/>
    <dbReference type="OMA" id="EHKHEIR"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24301:SF8">
        <property type="entry name" value="OS02G0221900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24301">
        <property type="entry name" value="THROMBOXANE-A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="3">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT66194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="531" mass="58926" checksum="1AE644BA71C4E4DB" modified="2012-06-13" version="1">MAAITNCSIALVTSTNGHSAAASPTTAALLLLSLIIAFLAYFHLPFWAVRKVPGPPTRFPLGHLHLLAQHGPDILRAMAQEHGPIFRFHMGRQPLVMAASAELCKEVGIKRFRDIRNRSAPPPTAGSPLHRDALFLARDSAWASMRSTVVPLYQPARLAQLVPTMRASVDALVDAVDQDQGSYVPFSQLSLRLAIDIIGKTAFGIEFGLLSKQGTNGDDEARELLGEYERSMEFMKMDLSSSLSTILGLFLPCLQTPCKRLLRRVPGTADHKMEQNERRLCRRIDAIIAARRRRSSSPATALDFIAALLEDSRGRVAALEDRHVRALAYEHLIAGTKTTAFTLSSLVYLVSCHRPVEEKLLAELDAFGPQSQSPDADELHTKFPYLDQIIKESMRFHLVSPLIARETSEAVEIGGYLLPKGTCVWLAPGVLARDAAQFPDPDEFRPERFAADGEEERARHPYAHIPFGIGPRACVGHRFALQQVKLAVVGLYRHFVFRHSPDMESPVEFDFDLVLGFRHGVKLRAIRRTND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZE5</accession>
    <name>I1HZE5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100832347</name>
        <name evidence="9" type="ORF">BRADI_3g09940v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318359.1">
        <property type="nucleotide sequence ID" value="XM_024462591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZE5"/>
    <dbReference type="EnsemblPlants" id="KQJ94332">
        <property type="protein sequence ID" value="KQJ94332"/>
        <property type="gene ID" value="BRADI_3g09940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832347"/>
    <dbReference type="Gramene" id="KQJ94332">
        <property type="protein sequence ID" value="KQJ94332"/>
        <property type="gene ID" value="BRADI_3g09940v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SWQD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027462_0_2_1"/>
    <dbReference type="OMA" id="NSTEWMP"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Expansin-like EG45" evidence="7">
        <location>
            <begin position="7"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="8">
        <location>
            <begin position="132"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ94332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="217" mass="23735" checksum="C14DBF8641205B54" modified="2012-06-13" version="1">MPPAPWAGACGYGNLYSEGYGTRTAALSTVLFNDGAACGQCYKIACDRKVDPRWCKPGVTVTVTATNLCPPNNALPNDNGGWCNVPRPHFDMAQPAWEKIGVYRGGIIPVMYQRVPCVKKGGVRFKINGHDYFNLVLVTNVAAIGSIKSMDVKSSDSKDWAPMARNWGANWHSLAYLSGKMLSFRLTNSDGQTLEFNNVVPSGWKFGQTFASKLQFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1I3H5</accession>
    <name>I1I3H5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor IIF subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100846625</name>
        <name evidence="10" type="ORF">BRADI_3g22897v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TFIIF alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234660.1">
        <property type="nucleotide sequence ID" value="XM_010236358.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I3H5"/>
    <dbReference type="STRING" id="15368.I1I3H5"/>
    <dbReference type="EnsemblPlants" id="KQJ96402">
        <property type="protein sequence ID" value="KQJ96402"/>
        <property type="gene ID" value="BRADI_3g22897v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846625"/>
    <dbReference type="Gramene" id="KQJ96402">
        <property type="protein sequence ID" value="KQJ96402"/>
        <property type="gene ID" value="BRADI_3g22897v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846625"/>
    <dbReference type="eggNOG" id="KOG2393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="VTCGKTM"/>
    <dbReference type="OrthoDB" id="76676at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I3H5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001096">
        <property type="term" value="F:TFIIF-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008851">
        <property type="entry name" value="TFIIF-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011">
        <property type="entry name" value="TFIIF-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05793">
        <property type="entry name" value="TFIIF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="206"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="211"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="354"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="394"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="431"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="455"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ96402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="531" mass="58486" checksum="FC1C0F8A9A3A0F7A" modified="2012-06-13" version="1">MGSVDLVLKPACEGCGSPSDLYGTGCKHTTLCSSCGKSMALSRARCLVCSAPITNLIREYNVRANASTDKAFSIGRFVTGLPPFSKKKNAENKWSLHKEGLQGRQLTDKMQEKYNRKPWILEDETGQYQFQGHMEGSQSATATYYLLMMQGKEFHAFPAGSWYNFSKVAQYKQLTLEEAEEKMNKRKTSATGYERWMMKAATNGPAAFGSDIKKLEPTNDGEKESARPKKGKDNEEGNNSDKGEENEEDEAARKDRLGLSKRGMDDDEEGGKDLDFDLDDEIEKGDDWEHEETFTDDDEAVDIDPEERADLAPEIPAPPEIKQDDEENEEEGGLSKSGKELKKLLGRASGQNESDADEDDEDDDQDDESSPVLAPKQNDQPKDEPVDNSPAKPTPSSGHARSTPPASKSKQKRKSGGDDAKTSGSAASKKAKVEPDTKTSSIKEEAPSSLKHTSKASASSRNANVSPVTEDEIRTVLLAVAPVTTQDLVSRFKSRLRGPEDKNAFAEILKKISKIQKTNGHNYVVLREDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1I3L5</accession>
    <name>I1I3L5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein TIFY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Jasmonate ZIM domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100825629</name>
        <name evidence="8" type="ORF">BRADI_3g23180v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Repressor of jasmonate responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The jas domain is required for interaction with COI1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TIFY/JAZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316337.1">
        <property type="nucleotide sequence ID" value="XM_024460569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I3L5"/>
    <dbReference type="STRING" id="15368.I1I3L5"/>
    <dbReference type="EnsemblPlants" id="KQJ96453">
        <property type="protein sequence ID" value="KQJ96453"/>
        <property type="gene ID" value="BRADI_3g23180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825629"/>
    <dbReference type="Gramene" id="KQJ96453">
        <property type="protein sequence ID" value="KQJ96453"/>
        <property type="gene ID" value="BRADI_3g23180v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R3M9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051749_3_1_1"/>
    <dbReference type="OMA" id="WGIEISD"/>
    <dbReference type="OrthoDB" id="694307at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018467">
        <property type="entry name" value="CCT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040390">
        <property type="entry name" value="TIFY/JAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010399">
        <property type="entry name" value="Tify_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077:SF124">
        <property type="entry name" value="PROTEIN TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09425">
        <property type="entry name" value="Jas_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06200">
        <property type="entry name" value="tify"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00979">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51320">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Tify" evidence="7">
        <location>
            <begin position="61"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ96453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="150" mass="15968" checksum="5051D99D771B0C61" modified="2012-06-13" version="1">MAVPAATTGATTSRFAAACDALSLYMKKSADKSRMQPSAVPVRPLPLMPGADVVADDDIREEPAQAQLTIFYGGRVVVLEDCPADKAAALVRLAAAAAAAAPEKKKKELDLPVARKASLQQFMDKRKARLAARAKPYRRADDADYLALAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8P3</accession>
    <name>I1I8P3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100822558</name>
        <name evidence="5" type="ORF">BRADI_3g40580v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572404.1">
        <property type="nucleotide sequence ID" value="XM_003572356.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8P3"/>
    <dbReference type="FunCoup" id="I1I8P3">
        <property type="interactions" value="1244"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I8P3"/>
    <dbReference type="EnsemblPlants" id="KQJ99019">
        <property type="protein sequence ID" value="KQJ99019"/>
        <property type="gene ID" value="BRADI_3g40580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822558"/>
    <dbReference type="Gramene" id="KQJ99019">
        <property type="protein sequence ID" value="KQJ99019"/>
        <property type="gene ID" value="BRADI_3g40580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822558"/>
    <dbReference type="eggNOG" id="KOG1267">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021017_1_1_1"/>
    <dbReference type="OMA" id="IHRCLAF"/>
    <dbReference type="OrthoDB" id="764594at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000019">
        <property type="entry name" value="mTERF family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000014">
        <property type="entry name" value="Transcription termination factor MTEF18, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF38">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ99019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="648" mass="73310" checksum="1C54C369283DFAA2" modified="2012-06-13" version="1">MSNSLRAAALRAARLGCARRIGLVADRGEPSSRVLPPLWRAAGSSRVRESAAAAAGWFPAVGHGIGLRPYSAAPRRRRKKTLVNDDGVEEEDDDEDEELRGVKQMQRRRDVRAAQRTLMEYLHVTRGMCFSDAEHISKRSPVFASKLLEKVKDAAREPAEGGDEVVFKSVVKKRDMKDERVSKALVRLFNYNPINEFEPFLESIGLSQSECSSFLPRDLMFLSDDELLFENYRVLCNYGIARCKIGKIYRDATEVFGFGHGVLVSKLNDIEELGFSKTSVIKLVTATPVVLVRDPNVELKILQWLDGIGIQWDWISQFLSARKSYNWTKMNQVPQFFSDLGFTKEGIAKLVRQHPDFLLDGSGKVLFTLVLIMLKAGSGKKELFDLFLNFPDVPVENFTKNLRKGMLFLAEVGLSNEDIKKIVLSDGQMLGSAPIKKPNSILTHLNTGKKRLRKIILENPKLLGSYRLGSKVSQLPRIDPFEQSFKGKIKFLKSIGFVEGSEEMKKALKVFRGKGDELQDRYDFLVNAGFDPKDVVNMIKMAPQILNQKIDVVESKISFLLNDTGYPLSELVCFPAYLSFTVERTKVRLFMYNWLLERGAVPQLALSTVLACSDKCFMRYYVKKHPMGPEVWENYKRESAEAKNMPCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IBD1</accession>
    <name>I1IBD1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isochorismatase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100840144</name>
        <name evidence="3" type="ORF">BRADI_3g48140v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575313.1">
        <property type="nucleotide sequence ID" value="XM_003575265.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IBD1"/>
    <dbReference type="STRING" id="15368.I1IBD1"/>
    <dbReference type="EnsemblPlants" id="KQK00242">
        <property type="protein sequence ID" value="KQK00242"/>
        <property type="gene ID" value="BRADI_3g48140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840144"/>
    <dbReference type="Gramene" id="KQK00242">
        <property type="protein sequence ID" value="KQK00242"/>
        <property type="gene ID" value="BRADI_3g48140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840144"/>
    <dbReference type="eggNOG" id="ENOG502QR6S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100758_0_0_1"/>
    <dbReference type="OMA" id="FCERNWP"/>
    <dbReference type="OrthoDB" id="2013482at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008936">
        <property type="term" value="F:nicotinamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019365">
        <property type="term" value="P:pyridine nucleotide salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044717">
        <property type="entry name" value="NIC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47297">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47297:SF2">
        <property type="entry name" value="OS02G0606800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="2">
        <location>
            <begin position="37"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK00242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="248" mass="26964" checksum="1BE21CA779DF2CAD" modified="2012-06-13" version="1">MGSEAAAAAIDLLRSAAPVLPDGDLLLTPREAPAAGLVLVDVSNGFCTVGAGNLAPVTPNKQIEKMVEESARLAKVFCERNWPVFAFLDTHYPDKPEPPFPPHCIVGSGEENFVPALEWLENDPNVTIRRKDCIDGYLAAFEKDGSNVFSDWIAKFQIKTVLVLGICTDYCVLDFASSTLAARNIGRVPPLEDIVIYSEGCATFDLPVEVARSIKGALAHPQDLMHHMGLYMAKSRGAKIVDRIILEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDS0</accession>
    <name>I1IDS0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation initiation factor eIF2B subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">eIF2B GDP-GTP exchange factor subunit epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100823064</name>
        <name evidence="9" type="ORF">BRADI_3g54980v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B gamma/epsilon subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570367.1">
        <property type="nucleotide sequence ID" value="XM_003570319.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IDS0"/>
    <dbReference type="FunCoup" id="I1IDS0">
        <property type="interactions" value="2594"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IDS0"/>
    <dbReference type="EnsemblPlants" id="KQK01294">
        <property type="protein sequence ID" value="KQK01294"/>
        <property type="gene ID" value="BRADI_3g54980v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823064"/>
    <dbReference type="Gramene" id="KQK01294">
        <property type="protein sequence ID" value="KQK01294"/>
        <property type="gene ID" value="BRADI_3g54980v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823064"/>
    <dbReference type="eggNOG" id="KOG1461">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012507_1_0_1"/>
    <dbReference type="OMA" id="LAQSCKI"/>
    <dbReference type="OrthoDB" id="424572at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IDS0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04197">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05787">
        <property type="entry name" value="LbH_eIF2B_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11558">
        <property type="entry name" value="W2_eIF2B_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000022">
        <property type="entry name" value="Translation initiation factor eIF-2B epsilon subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000106">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000029">
        <property type="entry name" value="Trimeric LpxA-like enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035543">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051956">
        <property type="entry name" value="eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056764">
        <property type="entry name" value="LbH_EIF2B3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044123">
        <property type="entry name" value="W2_eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25084">
        <property type="entry name" value="LbH_EIF2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="8">
        <location>
            <begin position="561"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="443"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="522"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="454"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK01294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="733" mass="81531" checksum="FAD909A908AB8088" modified="2012-06-13" version="1">MSQKKSRGGAAGAGDDAEDLSRSPLQAVLLADSFTLKFRPITLERPKVLLPLVNVPMIDYTLSWLETAGVEEVFVFCCAHAQQVKEHLEDAGWTGQPAAREMAVTAVESHDAISAGDALRVMYGRGVIHGDFILISGDTVSNMSLKDALQEHKDRRKKDPLAVMTMIIKHSKPSILTHQTRLGNDEIVMAIAPETKELLYYEDRADGSHLCVTIDKDILASNPTLQLHNNMEDCYIDICSPEVLSLFTDNFDYQHLRRHFVKGLLVDDIMGYKIYTHEICSSYAARIDNFRSYDAVSKDIIQRWTYPMVPDVLSFGNCHEVKLHRQGIYKASDVTLSHSAQIGANSVIGNATSIGEHCKISNSVIGAGCCIGKNVIIHGSYIWDNVIIEDGCKVSNSLVGDDVHLRAGAIVEPGCILSFKIKVGKNVVVPAYSKVSLLEKPSNEDSDEELEYADTNSGVTDSPPFSSMRSNAGRPAVPSKDDDLGASETGTSGVLGYIWASSDTGTQEDWRQSIAPIPKEKLQEMQHAVSVDDDNGSEEDLNNRQYEQDHDGDSEISVVEDDDYTKFEKEVEETFQRALDGIHEDNLILEINALRLSYSLQHADCAGAVFYSVMRSASVAAQSTNDNLLKTTADALAKWKDLLRNYTKTVDEEMEILLKFEEMCQETTKEFCSLFSKILPYLYDKDVVSEDAILRWAEEKENADEADKVFVKQSDAFIQWLKEAEEEDDDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEL3</accession>
    <name>I1IEL3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100844578</name>
        <name evidence="14" type="ORF">BRADI_3g57230v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in plant defense signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570518.1">
        <property type="nucleotide sequence ID" value="XM_003570470.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEL3"/>
    <dbReference type="STRING" id="15368.I1IEL3"/>
    <dbReference type="EnsemblPlants" id="KQK01632">
        <property type="protein sequence ID" value="KQK01632"/>
        <property type="gene ID" value="BRADI_3g57230v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844578"/>
    <dbReference type="Gramene" id="KQK01632">
        <property type="protein sequence ID" value="KQK01632"/>
        <property type="gene ID" value="BRADI_3g57230v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844578"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_21_2_1"/>
    <dbReference type="OMA" id="NQRYARS"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000228">
        <property type="entry name" value="Serine/threonine-protein kinase BIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="80"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK01632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="426" mass="46602" checksum="D48B5D10A9DE8971" modified="2012-06-13" version="1">MRALLFSCFAPRADDSPEQDSKTPTTPAAATTTKKKSVRRLRSATGRLRSLSLDDLSRTLASSGLHAFTVSELRAATRGFSGSSVIGEGGFGPVYKGFIDDRILPGSGLEPQQVAVKCLDAEGPQGHREWLAEVVYLGMQLRHPHLVKLVGYCCQDHHRILVYEYMARGSLEHHLFKNLLSSLPWATRLKIAVGAAKGLAFLHEAETPVIYRDFKASNILLESDYTAKLSDFGLAKEGPQGDDTHVSTRVMGTHGYAAPEYILTGHLTSRSDVYSYGVVLLELLTGRQSVDRRRRGREQNLVDWARPYLRRPDKLHRVMDPGLEGSYSDSAAAKAAMVAYTCLHSVPKSRPRMREVVELLEPLLEMRGDVPAGTFVYTAQPSVKKVQAPAVVVVVGDDKADGGGDRCVLPRGAGGEPPRQSRDRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IFL4</accession>
    <name>I1IFL4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RIC1 C-terminal alpha solenoid region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100824603</name>
        <name evidence="5" type="ORF">BRADI_3g60200v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK02074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236164.1">
        <property type="nucleotide sequence ID" value="XM_010237862.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IFL4"/>
    <dbReference type="FunCoup" id="I1IFL4">
        <property type="interactions" value="2890"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IFL4"/>
    <dbReference type="EnsemblPlants" id="KQK02074">
        <property type="protein sequence ID" value="KQK02074"/>
        <property type="gene ID" value="BRADI_3g60200v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824603"/>
    <dbReference type="Gramene" id="KQK02074">
        <property type="protein sequence ID" value="KQK02074"/>
        <property type="gene ID" value="BRADI_3g60200v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824603"/>
    <dbReference type="eggNOG" id="KOG2006">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002060_0_0_1"/>
    <dbReference type="OMA" id="MVYDRAM"/>
    <dbReference type="OrthoDB" id="67540at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034066">
        <property type="term" value="C:Ric1-Rgp1 guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000735">
        <property type="entry name" value="Quinoprotein amine dehydrogenase beta chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040096">
        <property type="entry name" value="Ric1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009771">
        <property type="entry name" value="RIC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746:SF10">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR SUBUNIT RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746">
        <property type="entry name" value="RAB6A-GEF COMPLEX PARTNER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25440">
        <property type="entry name" value="Beta-prop_RIC1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07064">
        <property type="entry name" value="RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIC1 C-terminal alpha solenoid region" evidence="4">
        <location>
            <begin position="757"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="600"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK02074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1126" mass="126368" checksum="BBCC1483E5AA20B7" modified="2012-06-13" version="1">MYMAYGWPQSIPLDPGDSDGGVVLLRVLGRLLLAVCPASLHLWSASQHKVRLARLDRSPDSLAAHGHNARAVWSPDAKTIAVLTSSFYLHIYKVQLSGKPLIVGGKQLPGLCLASLSLIIVEKVPLGNDISITSNFACDSKSMLLGLSNGHLQVVSWNAEFLDSFKLCCSTCSSEKTTAVVDALVFDPPSSRENSNARPAPCCTEDSAIFHVELSVKLRLLVALYSDCQIALCTVGKKGLKQTSGIRVERWLNTGDAMCTSVASDQQILAVGCSRGVVELYDLAENARHIRTVSLYDWGYSVEDTGPVTCISWTPDNCAFAVGWKFRGLTVWSVSGCRLMCTIRQAGSNSASSPMVKPNAQKFEPLMGGTSHIQWGDYGYKLFAVEESLSERVLAFSFAKCCLNRGLSSTTYTRQILYGEDRILLVQPDDTDELKMLHLNVPVSYSSQNWPVQHVVASDDGMYLAVAGSHGLVLYDLRNKRWRFFGDVTQEQKIQCKGLLWLGKVVIVCNYVESSNTYELLFFPRYHLDHSSLLYRKPLLGRPIVMDVFQDYILVTYSPFDVHIFHVMISGELSPTSSPVLQLSTVRELSIMSPKGPPVSMRLIPEPTDEGELKRDTDGSSDLLSQQPSRCLILRVNGELSVLDMDDGHEQALTNSVELFWVTCSQMEEKGNLIKEVSWLDYGHQGMQVWYPSHGANPFRQEDFLQLDPELEFDREVYPLGLLPNVGVVVGVSQRMSFSTAEFPCFEPSPQAQTILHCLLRHLLQRDKHEEALRLANLSAEKPHFSHCLEWLLFTVFDADISRPSTSKNQISPKSESPKRSLLEKTCDLLRNFSEYMDVVVSVARKTDGRHWADLFSAAGRSTEMFEECFQRRWYRTAACYILVIAKLEGPAVSQYCALRLLQATLDESLYELAGELVRFLLRSGRDFENANADSEKLSPRFMGYFLFRSPYKRQSSDLKSNSTKELSPHIASVMNILESHASYLMSGKELSKLVAFVKGTQFDLVEYLQRERLGSARLENFASALELIGQKLQMDTLQSRLDAEFLLAHMCSVKFKEWIVVLATLLRRAEVLVDLFRHDLRLWNAYSITLQSHDVFREYLDLLSALEDELSSVSNLTLQQNEQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2023-06-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>I1IG32</accession>
    <name>I1IG32_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g00630v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IG32"/>
    <dbReference type="EnsemblPlants" id="KQJ85616">
        <property type="protein sequence ID" value="KQJ85616"/>
        <property type="gene ID" value="BRADI_4g00630v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ85616">
        <property type="protein sequence ID" value="KQJ85616"/>
        <property type="gene ID" value="BRADI_4g00630v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2674492_0_0_1"/>
    <dbReference type="InParanoid" id="I1IG32"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ85616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="75" mass="7231" checksum="8C4163D6920CADD4" modified="2012-06-13" version="1">MAVVGGPDLGLGGLRRLAVVGRGPGCFGGGAGCRLGRSSTGVVCSGDGGPPLILAGGRRPWPTECPSQAGEAVDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IJY6</accession>
    <name>I1IJY6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100841151</name>
        <name evidence="4" type="ORF">BRADI_4g12070v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237500.1">
        <property type="nucleotide sequence ID" value="XM_010239198.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IJY6"/>
    <dbReference type="STRING" id="15368.I1IJY6"/>
    <dbReference type="EnsemblPlants" id="KQJ87583">
        <property type="protein sequence ID" value="KQJ87583"/>
        <property type="gene ID" value="BRADI_4g12070v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841151"/>
    <dbReference type="Gramene" id="KQJ87583">
        <property type="protein sequence ID" value="KQJ87583"/>
        <property type="gene ID" value="BRADI_4g12070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841151"/>
    <dbReference type="eggNOG" id="KOG4178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_2_1"/>
    <dbReference type="OMA" id="VYAREWL"/>
    <dbReference type="OrthoDB" id="7130006at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="29"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ87583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="312" mass="35525" checksum="EF61D7E8A6255C39" modified="2012-06-13" version="1">MTQEIEHTHLPIRGLNLHVAQVGKDELGTVVFLHGFPEIWYTWRHQMLAVAAAGYRAIAPDSRGYGLSDQPPEDVEATWEDLVADVLAILDTLSIQKVFLVGKDYGAIPAYDFALRHPDRTRGVTCLGIPFSPAPFDFTTMPEGFYILRWREPGGRAEADFGRHDVRRVVRTIYILFSRAEVPIAEEGQEIMDLADLSTPLPPWFTEEDLDAYAALFEKSGFRYPLQIPYRALHRMKMQLDAKFQVPVLMVMGEKDYCFKFPGFEEAMRSGAMETFAPDLKIVYIPEGSHFVQEQFPEQVNDLLLGFLKDHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1ILD1</accession>
    <name>I1ILD1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100841872</name>
        <name evidence="3" type="ORF">BRADI_4g17080v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576007.1">
        <property type="nucleotide sequence ID" value="XM_003575959.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ILD1"/>
    <dbReference type="STRING" id="15368.I1ILD1"/>
    <dbReference type="EnsemblPlants" id="KQJ88328">
        <property type="protein sequence ID" value="KQJ88328"/>
        <property type="gene ID" value="BRADI_4g17080v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841872"/>
    <dbReference type="Gramene" id="KQJ88328">
        <property type="protein sequence ID" value="KQJ88328"/>
        <property type="gene ID" value="BRADI_4g17080v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841872"/>
    <dbReference type="eggNOG" id="ENOG502S0SB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_683992_0_0_1"/>
    <dbReference type="OMA" id="HVSYQVC"/>
    <dbReference type="OrthoDB" id="1068731at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04051">
        <property type="entry name" value="C2_SRC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044750">
        <property type="entry name" value="C2_SRC2/BAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF169">
        <property type="entry name" value="PROTEIN SRC2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ88328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="403" mass="42683" checksum="D3EFC7220EAD133F" modified="2012-06-13" version="1">MADAGGGAGRRITVRSVSCRGVKSFVPFQKPPLYAAVSHGGRREKTPADPDGGENADWDGAVFAFDLDGRDDDGLLRFEVKAQVPLLGTKLVGSACVPVADLAGAGAPRRASYQLLAPDGKPNGSLSFVCSVTDAPAVGYQQQPQIYAARPEQDLNNSCCAPPPPPPSSWAPYPAPGMPNAAPHGSGYPPSAPPASAPLYPPLQDFLPSSYPPPPPRPTTDPLFPAPNFGSHSSYPRPPTVYPPPPASCTACPAPPAQYISSSYPPPPPSYYPPPPPSGYPPPARMNPEYPASTFPPTACSYPPPAPQPQPEFCSECPPSPAPRSVDRALHYMAPSSQGSAITHPELAVPQLPSGGSYYPPPGTRHREEEARPPYYYPPPGTRHREEEAKPPYYYPPPGTRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMW5</accession>
    <name>I1IMW5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100827097</name>
        <name evidence="13" type="ORF">BRADI_4g23610v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576215.1">
        <property type="nucleotide sequence ID" value="XM_003576167.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IMW5"/>
    <dbReference type="FunCoup" id="I1IMW5">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IMW5"/>
    <dbReference type="EnsemblPlants" id="KQJ89118">
        <property type="protein sequence ID" value="KQJ89118"/>
        <property type="gene ID" value="BRADI_4g23610v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827097"/>
    <dbReference type="Gramene" id="KQJ89118">
        <property type="protein sequence ID" value="KQJ89118"/>
        <property type="gene ID" value="BRADI_4g23610v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827097"/>
    <dbReference type="eggNOG" id="ENOG502QQH0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010485_1_0_1"/>
    <dbReference type="OMA" id="NREAMLC"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000084">
        <property type="entry name" value="probable methyltransferase PMT23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF1095">
        <property type="entry name" value="PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="98"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="277"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ89118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="870" mass="95818" checksum="4F1CAB63ED773236" modified="2012-06-13" version="1">MALFDRNQRGQRSSLFSTATIVLFVALCLVGFWMVSTPSAPPEALPTTTTASAAAEVVKKADAAGAAKEKEEDSSIDATNNFKQDSTNVVAAEVVATNAETNNPDNGGGGDGDKAASFDDENGRTEGGELVKPETTADADSAAAAAAVAARKTVDDTTTAKDGEKTSGDTKNSDESTVSAASKKQTFDDENGKMEGVDVVKDDAGANNSNKTFISEDITVKPIADESSTAAEAKLTSSDSTGEQQALQEEDQMNLLPEALPNGQAELLTERAAQNGSFTTQADESTNEKNKRAAELKNSTTKKTKKKKAKKPKGANNNKNNGTSSLSSSSSTTTVSWPYAWKLCNTSAGADYIPCLDNEAAISKLKTNKRYEHRERHCPSTPPTCLVPSPAAYREPIRWPASRSKIWYHNVPHASLASYKHNQNWVKLSGEHLVFPGGGTQFKTGGALHYIDLIQEALPEVAWGRRSRVVLDVGCGVASFGGFLFDRGALTMSFAPKDEHEAQVQFALERGIPALSAVMGTKRLPFPAGVFDVVHCARCRVPWHIDGGMLLLELNRLLRPGGFFVWSATPVYQKLPEDVEIWDDMVKLTKAMCWEMVKKTEDTLDQVGLVIFRKPKSNRCYETRRQKEPPLCDGSDDPNAAWNIKLRACMHRAPADYPSVRGSRWPAPWPERAEAVPYWLNNSQVGVYGRPAREDFAADYEHWRKVVQNSYLTGMGIDWAAVRNVMDMRAVYGGLAAALRDMSVWVMNTVTIDSPDTLPVIFERGLFGIYHDWCESFSTYPRSYDLLHADHLFSKLKTRCKVLPVIVEADRILRPNGKLIVRDDKETVNEIVELVRSMHWEVRMTVSNRKEAMLCARKTMWRPTEVEARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1IS36</accession>
    <name>I1IS36_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">30S ribosomal protein S31, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100821448</name>
        <name evidence="5" type="ORF">BRADI_4g35920v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bTHX family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578489.1">
        <property type="nucleotide sequence ID" value="XM_003578441.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IS36"/>
    <dbReference type="STRING" id="15368.I1IS36"/>
    <dbReference type="EnsemblPlants" id="KQJ91153">
        <property type="protein sequence ID" value="KQJ91153"/>
        <property type="gene ID" value="BRADI_4g35920v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821448"/>
    <dbReference type="Gramene" id="KQJ91153">
        <property type="protein sequence ID" value="KQJ91153"/>
        <property type="gene ID" value="BRADI_4g35920v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821448"/>
    <dbReference type="eggNOG" id="ENOG502S834">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154125_0_0_1"/>
    <dbReference type="OMA" id="QPCASRF"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032544">
        <property type="term" value="P:plastid translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030826">
        <property type="entry name" value="Ribosomal_bTHX/bTHXc/bTHXm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044695">
        <property type="entry name" value="Ribosomal_bTHXc/bTHXc_plant"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04560">
        <property type="entry name" value="ribo_THX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34550">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S31, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34550:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BTHXM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17067">
        <property type="entry name" value="RPS31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ91153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9250" checksum="7F1AC0377536CA19" modified="2012-06-13" version="1">MAMRMMAASFVRRLAPARPPALLAEVEAVTCGRGDKKTKRGKRFKGSYGNARPKREKKIERIKDRVEVPRSTPWPLPFKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IUG4</accession>
    <name>I1IUG4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100825464</name>
        <name evidence="7" type="ORF">BRADI_4g42842v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578925.1">
        <property type="nucleotide sequence ID" value="XM_003578877.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IUG4"/>
    <dbReference type="FunCoup" id="I1IUG4">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IUG4"/>
    <dbReference type="EnsemblPlants" id="KQJ92317">
        <property type="protein sequence ID" value="KQJ92317"/>
        <property type="gene ID" value="BRADI_4g42842v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825464"/>
    <dbReference type="Gramene" id="KQJ92317">
        <property type="protein sequence ID" value="KQJ92317"/>
        <property type="gene ID" value="BRADI_4g42842v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825464"/>
    <dbReference type="eggNOG" id="KOG0543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013615_4_0_1"/>
    <dbReference type="OMA" id="ICVASMK"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IUG4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000708">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039663">
        <property type="entry name" value="AIP/AIPL1/TTC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242">
        <property type="entry name" value="ARYL HYDROCARBON RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242:SF0">
        <property type="entry name" value="TPR_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <keyword evidence="2 4" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="6">
        <location>
            <begin position="79"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="276"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ92317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="375" mass="42517" checksum="4664CFE35FE895DA" modified="2012-06-13" version="1">MAVATEDAAPAPAVPAAASDPAAGPIDDEITVEETSFVHTEPPQDDNAPPVVTTDTEVLHDKVKKQVIKEGHGKTPSKFSTCFVHYRAWVQSSLHKFEDTWQEQHPIEIVIGKEKKQMTGLGIGVGNMRSGERALLHVGWELGYGKEGSFSFPNVPPTADLIYEVELIGFDDAKEGKARSDMTVEERIEAADRRKLEGNDYFKEKKFEEAMQQYEMAVAYMGDDFMFQLFGKYRDMALAVKNPCHLNMAACLIKQKRFDEAIAQCSIVLSEDEINVKALFRRGKARAELGQTESAREDFLKAKKHAPEDKEILRELRLLAEHDKAIYQKQKEMYKGLFGPRPQPKPKARNFILLFWQWLVSLIRNLAKIFTRKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I1IV36</accession>
    <name>I1IV36_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g44630v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IV36"/>
    <dbReference type="EnsemblPlants" id="KQJ92586">
        <property type="protein sequence ID" value="KQJ92586"/>
        <property type="gene ID" value="BRADI_4g44630v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92586">
        <property type="protein sequence ID" value="KQJ92586"/>
        <property type="gene ID" value="BRADI_4g44630v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1588722_0_0_1"/>
    <dbReference type="InParanoid" id="I1IV36"/>
    <dbReference type="OMA" id="CHYHLEN"/>
    <dbReference type="OrthoDB" id="624742at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47186:SF22">
        <property type="entry name" value="OS11G0589401 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="2">
        <location>
            <begin position="14"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="168" mass="19323" checksum="00DDEE5B555659DD" modified="2012-06-13" version="1">MDEEPNSDKTEIRQVRSFNAIMCDVKRGSFLSSFQGLRVLSMESCRLMNTCHYHLENLGRLLQLRYLGLLETPITELLEEIGNLRFLQVLELRKIGIKELPQSVGQLRLLKCLCLPTDFTGAPGWIGNLVLLEELLLPYVSLEIVKELAKLTELRDTTRHTPWSHGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVB8</accession>
    <name>I1IVB8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106866906</name>
        <name evidence="5" type="ORF">BRADI_4g45330v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758839.1">
        <property type="nucleotide sequence ID" value="XM_014903353.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVB8"/>
    <dbReference type="EnsemblPlants" id="KQJ92702">
        <property type="protein sequence ID" value="KQJ92702"/>
        <property type="gene ID" value="BRADI_4g45330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="106866906"/>
    <dbReference type="Gramene" id="KQJ92702">
        <property type="protein sequence ID" value="KQJ92702"/>
        <property type="gene ID" value="BRADI_4g45330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:106866906"/>
    <dbReference type="eggNOG" id="ENOG502RY4T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121137_0_0_1"/>
    <dbReference type="OMA" id="AEQQHYY"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="CCT_domain"/>
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        <property type="entry name" value="CONSTANS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319:SF114">
        <property type="entry name" value="OS12G0262400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
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    </dbReference>
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    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="82"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ92702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="194" mass="22014" checksum="ECBAFEFB6543720B" modified="2012-06-13" version="1">MYHHSSSSSYYCQGSNGFLPSQQSASSYSDLLQAEQQHYYSQRQQQQEPMQAQALFRRVLSTGDLGTPPSAPVAGKYSMEERRERIEKYRNKRNQRNFQKKITYACRKTLADSRPRVKGRFARNVDDVSDQQEDAATSEVSSIDSLVMNEANVVVNATDAASSSSMPEWWPAMQGALEMEDEELYLAVSTINLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I1IXM7</accession>
    <name>I1IXM7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Extensin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_5g09800v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IXM7"/>
    <dbReference type="STRING" id="15368.I1IXM7"/>
    <dbReference type="EnsemblPlants" id="KQJ82581">
        <property type="protein sequence ID" value="KQJ82581"/>
        <property type="gene ID" value="BRADI_5g09800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82581">
        <property type="protein sequence ID" value="KQJ82581"/>
        <property type="gene ID" value="BRADI_5g09800v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R669">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1095586_0_0_1"/>
    <dbReference type="InParanoid" id="I1IXM7"/>
    <dbReference type="OMA" id="HITRQYP"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095585" description="Extensin domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ82581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="26772" checksum="F2C8CF8009CEED67" modified="2012-06-13" version="1">MATTNMKSLALLLALAATLLSCCTASSPAAAPAWAPDADGASPPQQYTFPPVAAPAPAAYGEPPMTAFPAPQSGKPHHHANSPVAEPPVYHNGSEEHGKHHHHGNSPAAEPPVYHNGSEEHGKHHHHHGKKSPAEPPAYHNGTEHKKHHGHLGHHHQQHHDAPPSPSPAHHAPPPPAHHSPPPPAHQHLPPPPPSRYPPHFPAPPAHKYPPPPAHHGKAPPPPPPRGHRKFPPQSGYSAMAPSPSYDGAEATSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I1J3L2</accession>
    <name>I1J3L2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BRADI_5g26850v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function as sodium-coupled metabolite transporter across the chloroplast envelope.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast envelope</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J3L2"/>
    <dbReference type="STRING" id="15368.I1J3L2"/>
    <dbReference type="EnsemblPlants" id="KQJ85408">
        <property type="protein sequence ID" value="KQJ85408"/>
        <property type="gene ID" value="BRADI_5g26850v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ85408">
        <property type="protein sequence ID" value="KQJ85408"/>
        <property type="gene ID" value="BRADI_5g26850v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1498305_0_0_1"/>
    <dbReference type="InParanoid" id="I1J3L2"/>
    <dbReference type="OrthoDB" id="1938757at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045158">
        <property type="entry name" value="KEA4/5/6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16254">
        <property type="entry name" value="POTASSIUM/PROTON ANTIPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16254:SF14">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="64"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ85408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="180" mass="20386" checksum="D924999D52AA0169" modified="2012-06-13" version="1">MRASYVQARSAAVLMGSPWASMRGSRKLQGEILFTSRPPARLFFFGMDFDGYVVYIKPTPHWEFGMLINVHFLRNHVDILLAAVILVITIKTFTVAIVVKGFGYSNKTSLLIGMSLAQIREFAFVLLSRASSILLIEVTTPLLFKMIPAVVHLGVLLRWFSVDTLISIRWRYTLISTEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EIE7</accession>
    <name>A0A0Q3EIE7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g11614v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EIE7"/>
    <dbReference type="EnsemblPlants" id="KQJ87519">
        <property type="protein sequence ID" value="KQJ87519"/>
        <property type="gene ID" value="BRADI_4g11614v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87519">
        <property type="protein sequence ID" value="KQJ87519"/>
        <property type="gene ID" value="BRADI_4g11614v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EIE7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ87519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="164" mass="17695" checksum="7EA8C3352FB56672" modified="2016-01-20" version="1">MPERQHVAEKQFVPEVLVEHVASADISAAIEVALKSLIPAQAELLCVELQKMATVCLDETVQPLHDIAASIQSLVLQLGTILERSEVTLGKLASVPVVQVEAAPDADASQASPTLLDPPKKLVVNFTDGGQTVILAIALLVAGLLLRLMRLIYLWHIPPSWCRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FTL0</accession>
    <name>A0A0Q3FTL0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g02173v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FTL0"/>
    <dbReference type="EnsemblPlants" id="KQK02543">
        <property type="protein sequence ID" value="KQK02543"/>
        <property type="gene ID" value="BRADI_2g02173v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02543">
        <property type="protein sequence ID" value="KQK02543"/>
        <property type="gene ID" value="BRADI_2g02173v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FTL0"/>
    <dbReference type="OrthoDB" id="686093at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK02543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="237" mass="24586" checksum="71A26821573FBD1C" modified="2016-01-20" version="1">MDQQDALAVHDLSAQGGDEGSMGPGRVLEVESSVHGATLESVKKGNHVGESSAPAPVTASGSGTKVAMAHASPPDSYRRVPSRSRKQYSPQRFIATQPAALDDDTPSRDRKHPRPEHFIPEEAEASAAAKSRRSNIVVDRFLSSSILHGSPTETSSEQAGGRVRGVGAAASRLGPACVTPGRASPSATAELNGSARIYIVIAILGASLALSVVSCLLFYLVGRRSGSEPGPPPGEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FZY9</accession>
    <name>A0A0Q3FZY9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF679 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_2g16640v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant DMP1 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FZY9"/>
    <dbReference type="FunCoup" id="A0A0Q3FZY9">
        <property type="interactions" value="660"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FZY9"/>
    <dbReference type="EnsemblPlants" id="KQK04895">
        <property type="protein sequence ID" value="KQK04895"/>
        <property type="gene ID" value="BRADI_2g16640v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04895">
        <property type="protein sequence ID" value="KQK04895"/>
        <property type="gene ID" value="BRADI_2g16640v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FZY9"/>
    <dbReference type="OrthoDB" id="525686at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FZY9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007770">
        <property type="entry name" value="DMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31621">
        <property type="entry name" value="PROTEIN DMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31621:SF0">
        <property type="entry name" value="PROTEIN DMP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05078">
        <property type="entry name" value="DUF679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK04895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="27285" checksum="3560E4232EE69608" modified="2016-01-20" version="1">MSSRRAVLSPGNILLRRSVTQDPAAAMATPRSEVVEEQPQPQPQEHGVHVEEEERQPLLVKRSADDGGTGMSPMQKAISQTYQSTAHLATLLPTGTVLAFQLLSPLVTNQGDCLPSNRAMAGALVSLCALSCFVLSFTDSFRDDKQTGNNGAVRYGFATLRGLWVIDGGAPLEASQAAKYRVRFLDLVHAVVSVMVFAAVALFDQNVVGCFCPVPSQDARQVLTVLPIAIGVVGSMLFVAFPTTRHGIGFPLSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GPK2</accession>
    <name>A0A0Q3GPK2_BRADI</name>
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        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">LOC100830360</name>
        <name evidence="3" type="ORF">BRADI_1g03142v3</name>
    </gene>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010227384.1">
        <property type="nucleotide sequence ID" value="XM_010229082.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GPK2"/>
    <dbReference type="EnsemblPlants" id="KQK12349">
        <property type="protein sequence ID" value="KQK12349"/>
        <property type="gene ID" value="BRADI_1g03142v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830360"/>
    <dbReference type="Gramene" id="KQK12349">
        <property type="protein sequence ID" value="KQK12349"/>
        <property type="gene ID" value="BRADI_1g03142v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830360"/>
    <dbReference type="OrthoDB" id="690324at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GPK2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AT5G49610-like_b-prop"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133:SF305">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32133">
        <property type="entry name" value="OS07G0120400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23635">
        <property type="entry name" value="Beta-prop_AT5G49610-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="14"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK12349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="474" mass="52894" checksum="92C62CFB73E4167C" modified="2016-01-20" version="1">MAPPPRRRDSPQELPDELIVEIFLRFPLDEPSCLLRASLVSRRLYRAVSDPFFRQRLHELHRTPPALGFLHNRDDEDIPRFVHSTASAFPLAAPDDHPSWEVVVDCRHGRALFLIHYQVARELLIWEPITGAQHRVPVPAAFENLCPTAAVFCPVEGCSHYNCIGGPFRVVFVFAVDDNDEAKFFTSASVYSSETGTWGEVTRILNRAVCFSSPYRSSVLVWRSLFYFMADGSDILEYDLDSHALARLESPDPYTDTFSLMVAEDGGLGVCKVSDPQQLKLWSWQESDDDDTDGQWVLSRVIYLGNLLPNGALPLSSLSKRVLFFAEVANVIFVTTDDGVFTIELQSERAKKVCDHHFWCLVPVVSFYTPLRLLNLSEEAGGEEGGVKEEKTVERAHQLFGKGTNAVKEGDFDHSFINHDLKTRSVSNEESVMGTSSKDDAGNSKISGSNIMDAASPSEKGDSEEGSKLNSDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GZ40</accession>
    <name>A0A0Q3GZ40_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g03897v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GZ40"/>
    <dbReference type="EnsemblPlants" id="KQJ86194">
        <property type="protein sequence ID" value="KQJ86194"/>
        <property type="gene ID" value="BRADI_4g03897v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ86194">
        <property type="protein sequence ID" value="KQJ86194"/>
        <property type="gene ID" value="BRADI_4g03897v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GZ40"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ86194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="42" mass="4366" checksum="0F092660865A07A0" modified="2016-01-20" version="1">MALAQCAHRWIPCSSPRRCASAGTSSGCAGEHAVHPVALLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H411</accession>
    <name>A0A0Q3H411_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g16196v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ88178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H411"/>
    <dbReference type="STRING" id="15368.A0A0Q3H411"/>
    <dbReference type="EnsemblPlants" id="KQJ88178">
        <property type="protein sequence ID" value="KQJ88178"/>
        <property type="gene ID" value="BRADI_4g16196v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88178">
        <property type="protein sequence ID" value="KQJ88178"/>
        <property type="gene ID" value="BRADI_4g16196v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H411"/>
    <dbReference type="OrthoDB" id="723086at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ88178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="123" mass="13958" checksum="0CEC8B7A0389136A" modified="2016-01-20" version="1">MGEQRITHLKNTTKLNYLSCMNKQSRAKWSKSDSDLFNYQARNNLYLKQVIKKNLNIKDLPIDVNSTRKTRAASNGGNSGNENVLEDSINHVEENGLNCWSKAQAGHRRIRCQGSGGPCFCKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H7Q2</accession>
    <name>A0A0Q3H7Q2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100830377</name>
        <name evidence="8" type="ORF">BRADI_1g46195v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312687.1">
        <property type="nucleotide sequence ID" value="XM_024456919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H7Q2"/>
    <dbReference type="STRING" id="15368.A0A0Q3H7Q2"/>
    <dbReference type="EnsemblPlants" id="KQK19055">
        <property type="protein sequence ID" value="KQK19055"/>
        <property type="gene ID" value="BRADI_1g46195v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK19056">
        <property type="protein sequence ID" value="KQK19056"/>
        <property type="gene ID" value="BRADI_1g46195v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT76233">
        <property type="protein sequence ID" value="PNT76233"/>
        <property type="gene ID" value="BRADI_1g46195v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830377"/>
    <dbReference type="Gramene" id="KQK19055">
        <property type="protein sequence ID" value="KQK19055"/>
        <property type="gene ID" value="BRADI_1g46195v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19056">
        <property type="protein sequence ID" value="KQK19056"/>
        <property type="gene ID" value="BRADI_1g46195v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76233">
        <property type="protein sequence ID" value="PNT76233"/>
        <property type="gene ID" value="BRADI_1g46195v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="660550at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF31">
        <property type="entry name" value="ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014034280" description="Peptidase A1 domain-containing protein" evidence="6">
        <location>
            <begin position="22"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="100"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK19056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="396" mass="41290" checksum="0362D3EFF4250562" modified="2016-01-20" version="1">MKAISVLQLLPYDLFLALSSASTPAAPAVRADLTHVDSGRGFTSRELLRRLATRSRARASRLYSSSSSSSSARPAGAGSHAVTAPLARGTVGDADIDSEYLIHLSIGTPRPQRVALTLDTGSDLVWTQCACHVCFAQPFPTFDALASQTTLAVPCSDPICTSGKYPLSGCTFNDNTCFYLYDYADKSITSGRIVEDTFTFRSPQGNNGSKAHAGVAVPNVRFGCGQYNKGIFKSNESGIAGFSRGPMSLPSQLKVARFSHCFTAIADARTSPVFLGGAPGPDNLGAHATGPVQSTPFANSNGSLYYLTLKGITVGKTRLPLNALAFAGKGTGSGSGGTIIDSGTGIRTLPGPMYRSLRAAFVARVKLPVANESAADAESTLCFEAARCRGAPSAIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HGQ0</accession>
    <name>A0A0Q3HGQ0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g64813v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HGQ0"/>
    <dbReference type="EnsemblPlants" id="KQK22055">
        <property type="protein sequence ID" value="KQK22055"/>
        <property type="gene ID" value="BRADI_1g64813v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22055">
        <property type="protein sequence ID" value="KQK22055"/>
        <property type="gene ID" value="BRADI_1g64813v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HGQ0"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000242">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF360">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="150"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="286"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="463"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK22055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="555" mass="62932" checksum="706150A3053A9C3D" modified="2016-01-20" version="1">MSYTNCLKKHPTRLFFPYANSILRASLEKGSPQKSLMDYGTMLNSTTFCPDYRTYGILLRACLKYSDIYAAMQIHSRLTKVGLLRNQHIIAPLLRLYIDYDCMTEARELFWLMLEWSTDPFHGNLMLTGFLKGGQLDKAYQIFKRMPVKDVVSWNSMIAGAVRSSHLKDAMILFSRLVNSGLVPDGFSFSSVLSACARAGALFYGVWVHRLMAELGVEKNNILISALVDMYAKCGRIDVSVEIFNTVKRNHVPMWNTMIGSLAAHGLGQDVVVLFHRMEHAGVVPDGVTFVALLTACSHCGMVEEARQYFEAMTTKYYITPKVEHYGAMVDTLSRAGLLDEAYNLVRSMVVKPDAVIWRALLSACRRHHQTKLGEITIEQMVCQGSGDYTLLSNIYSSINRWVDSENVWKERKKKKVRKNKGLSWVELGGSTHEFKAGDRSHPDTDDIYRVLHGLSKKAKAEGYSPSTELVMKDVSHEEREENLTFHSEKLAVAYSVLKTGPGTEIMVSKNLQTCGDCHEWIKIISKVLCRVIIMRDRARFHRFESGCCSCKDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IKP0</accession>
    <name>A0A0Q3IKP0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_4g07847v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IKP0"/>
    <dbReference type="EnsemblPlants" id="KQJ86808">
        <property type="protein sequence ID" value="KQJ86808"/>
        <property type="gene ID" value="BRADI_4g07847v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ86808">
        <property type="protein sequence ID" value="KQJ86808"/>
        <property type="gene ID" value="BRADI_4g07847v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843896"/>
    <dbReference type="OrthoDB" id="202537at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IKP0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18624">
        <property type="entry name" value="GH32_Fruct1-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Beta-fructofuranosidase, insoluble isoenzyme CWINV1"/>
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    </dbReference>
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        <property type="entry name" value="Exo-inulinase, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Fructan_Metab_Enzymes"/>
    </dbReference>
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        <property type="entry name" value="Glyco_hydro_32"/>
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        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31953">
        <property type="entry name" value="BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="7">
        <location>
            <begin position="81"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="8">
        <location>
            <begin position="413"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ86808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="643" mass="69896" checksum="0AFA5881922CA8CF" modified="2016-01-20" version="1">MSSGFCDFQNRILKLRIISMQVEGSLIDLIKSASLDGYLYLARRVVQFANQQFRPTHSFVSSPILAMDGTKNPQHGRTAYHFQPAKNWQNDPNGPMYHNGMYHLFYQYNPHGPTWDAGKLSWGHSVSGDLVNWAALDNALDPTSPFDANGCWSGSATVLPGGRPAILYTGIDADRVQVQNVAFASNPSDPLLRDWHKPSCNPVIGIPADVTGNNFRDPTEPWRGSDGLWRVAVAAEVEGKGTLLVYRSADFLRWERNPGPPLHASSPAVPVLECPDLFPISMAAAAEQEGLDALMAAGGSSSGGVTHVLKLTDFAKEDHYMVGRYDELAGDTFAPAEPERGDDPGRWRRLDHGHLYASKSFFDARKKRRVLWAWVDENDGAAEAKGWAGIQAFPRAMWLDGDGKGLVQWPVEEIETLRRKRDSGFGPEGTEVGAGGKVEIGAGIQSSQADVEVVFEIPSLEEAETLDDLEWALDDPQRLCAEKGAFVHGGVGPFGLLVLASGGLQEHTAVFFRVFRHQGKYKVLMCTDLTRSSTKAGVNKPCYGAFLDVDVEKDRSISLRTLIDHTVVESFGNGGRTCMTARVYPEHAAKGRSRTYVFNHGAGAVKVSKLEAWELAAAAVNGAGGGGGSVAPDVATVWSDFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IMJ1</accession>
    <name>A0A0Q3IMJ1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g33105v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IMJ1"/>
    <dbReference type="EnsemblPlants" id="KQK07107">
        <property type="protein sequence ID" value="KQK07107"/>
        <property type="gene ID" value="BRADI_2g33105v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37265">
        <property type="entry name" value="OS01G0195300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37265:SF5">
        <property type="entry name" value="OS01G0195300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK07107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="185" mass="19586" checksum="21AAB77C8BF30E38" modified="2016-01-20" version="1">MDQESAGGKKRKRKPGDNAGAPKRKPRNNTGARKSSNRRGSGAGEQQQQQAAAAIEKVAECVRLPEEEEEENYEGITEESIAEMMSWLALELVPPPAPAGRSCVSVQGSNMESCGPSFSGSASTVMASVDFRFAAPVPQVVPWPWPSPAAEAVPVAGPQGEEGMADDEWVAGLLTDGPPDEGLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IX22</accession>
    <name>A0A0Q3IX22_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein SCAI</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100833218</name>
        <name evidence="2" type="ORF">BRADI_4g32790v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IX22"/>
    <dbReference type="FunCoup" id="A0A0Q3IX22">
        <property type="interactions" value="1893"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3IX22"/>
    <dbReference type="EnsemblPlants" id="KQJ90608">
        <property type="protein sequence ID" value="KQJ90608"/>
        <property type="gene ID" value="BRADI_4g32790v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ90608">
        <property type="protein sequence ID" value="KQJ90608"/>
        <property type="gene ID" value="BRADI_4g32790v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="525027at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IX22">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022709">
        <property type="entry name" value="SCAI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21243">
        <property type="entry name" value="PROTEIN SCAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12070">
        <property type="entry name" value="SCAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ90608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="643" mass="71615" checksum="D4805A96C3230410" modified="2016-01-20" version="1">MILSLPEQTPLLLLSLSTTVTISPPPPPIRRPNRQTEDRERELVSPLLLSSPAMTDGEGSGEYREFKALVEAADRKFARARDLPLYGGGDHHSRKAFKAYTRLWRLQQERRRELVAAGLRRWEIGEVASRIGQLYYARYLRTAEPRSLVGAYVFYEAIYSRGYFAASAAGGGGAGSGGKHQGLLIRYKELRFIARFLVVAMLMRRAEAVDHLVGRLRSLLDETKSAYPKTNFKEWKQVLQELGRFLKADGAYKGARSLRYDNLFDSYASNLASIARFHSKRVLKLKEAVLTSYRRNEVKFTELTLDTFRMLQCLEWEPTGSYQIAVKELTENGTMSDQSGPSGLIDIHLSTEISDGSLPSNPQKAILYHPSVSHLLAVLATICDELSQDSILLIYISASGSADQNPCQKYASSSSSRAKPTAAFCTDKPNPHTASDNHLWLGPRGSGGPNILYPEDLIPFTRYPLFLVIDSENSHAFKAIHNAEKGEPAALLLSPRIVPAMPGADSMGNGSQFTYFLTAPMQAFCQLAGITSDIDSDTYANAENILFSAMEQYEETLCTSVGLNNVWGQILPDPFLRRLILRFIFCRAVLFYFHPEEHVEHLPTCLPSLPESVSPKAKAIKTPILLLAENLVVSNRFHFGNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J8F4</accession>
    <name>A0A0Q3J8F4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g10591v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_3g10591v3"/>
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        <property type="component" value="Chromosome 3"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ94448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="145" mass="16790" checksum="F7ABCF6674967069" modified="2016-01-20" version="1">MPLLICQFYFWMSLSISFLLSIQILSSFFLNIHILSSIVLRNLIQIENNSSFLNIHILSSIFLRNLIQIENISFLNIHILSSIFLRNLIQIGNNNSFLNIHILSNNFLSIHILRKNFLSIRILSNNFFLNNIFLSIRLGACHGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JP61</accession>
    <name>A0A0Q3JP61_BRADI</name>
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC112270552</name>
        <name evidence="3" type="ORF">BRADI_1g48910v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313977.1">
        <property type="nucleotide sequence ID" value="XM_024458209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JP61"/>
    <dbReference type="STRING" id="15368.A0A0Q3JP61"/>
    <dbReference type="EnsemblPlants" id="KQK19539">
        <property type="protein sequence ID" value="KQK19539"/>
        <property type="gene ID" value="BRADI_1g48910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112270552"/>
    <dbReference type="Gramene" id="KQK19539">
        <property type="protein sequence ID" value="KQK19539"/>
        <property type="gene ID" value="BRADI_1g48910v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="617902at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23340">
        <property type="entry name" value="beta-trefoil_FSCN_ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
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        <property type="entry name" value="DUF569"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054726">
        <property type="entry name" value="Ubiq_DUF569-assoc"/>
    </dbReference>
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        <property type="entry name" value="ACTIN CROSS-LINKING PROTEIN (DUF569)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31205:SF72">
        <property type="entry name" value="DUF569 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04601">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="DUF569" evidence="2">
        <location>
            <begin position="188"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK19539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="283" mass="31363" checksum="64F8E5DD1170341F" modified="2016-01-20" version="1">MDQFPNKEHVWVRSRALRTYLHASTDGTRVSLLPTRASINAAWAVQVHEAEHGSPCVLLQGAAYGRYLAATDLPAPFGHSGYRASQLEYCDIPVPEEESVLWRVIPAASGDDVLLRHSAGGRYLRANGNGRRNTGVTVEKIRSLTTMAQWEVEVIPANPNFPGIPGPIYEPVRRDRRILSTGRELPPLRLIRFVQADAEGEYNEDGWSQFSFRGNSIYQLRNEVANRVHINSFVMCVRAGLHAPLAPMLANLPSGRTGNTLYIVVIQSTTPAELAPRYPDINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JU91</accession>
    <name>A0A0Q3JU91_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100826916</name>
        <name evidence="10" type="ORF">BRADI_1g24190v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229637.2">
        <property type="nucleotide sequence ID" value="XM_010231335.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JU91"/>
    <dbReference type="EnsemblPlants" id="KQK15652">
        <property type="protein sequence ID" value="KQK15652"/>
        <property type="gene ID" value="BRADI_1g24190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826916"/>
    <dbReference type="Gramene" id="KQK15652">
        <property type="protein sequence ID" value="KQK15652"/>
        <property type="gene ID" value="BRADI_1g24190v3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF122">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0186">Copper</keyword>
    <keyword evidence="3 8" id="KW-0187">Copper transport</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK15652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="177" mass="18116" checksum="83256F02E8FE75EE" modified="2016-01-20" version="1">MSMPMPPPPPSHAGDITHMPGTDMGMGMGMAMPAMHATFFWAHRAQVLFTGWPGEGPRAGSGMYVLCLLVVLALAALVEAFSAASKGLARRRPGGGAMVSGVVVHAAKMALAYMVMLAAMSFNVGVLLAAVAGHALGFLLARTAVLRRAGAGGTRDDDAAAPQNVVAHATNESEPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K8V4</accession>
    <name>A0A0Q3K8V4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DCD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836442</name>
        <name evidence="3" type="ORF">BRADI_2g34547v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71726.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_024458330.1"/>
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        <property type="gene ID" value="BRADI_2g34547v3"/>
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        <property type="gene ID" value="BRADI_2g34547v3"/>
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        <property type="gene ID" value="BRADI_2g34547v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_2g34547v3"/>
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        <property type="protein sequence ID" value="PNT71726"/>
        <property type="gene ID" value="BRADI_2g34547v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT71727"/>
        <property type="gene ID" value="BRADI_2g34547v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836442"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="NRP-like"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46034:SF7">
        <property type="entry name" value="INFLUENZA VIRUS NS1A-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="6"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="11"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="426"/>
        </location>
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        <location>
            <begin position="458"/>
            <end position="479"/>
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        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="419"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51222"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="858" mass="93963" checksum="0DC3AE244317D7CD" modified="2016-01-20" version="1">MQVQTRNLRENQLGGVIFGCKHETMEECFSKQLFGLPSLHLSYVRNVKAGLPLFLFNYSDRKLHGVFEAASPGGNCIDPYAWSDDGTLRTPFPAQVRICTRTRYLPLLEGQYKKVLQDNYYTSHYFFFELDHAQTRALIALFKSLGPPNVKQVPAISSKRSLVVPLPSTKRVASVIPKQKKGTSNSKDINPFSVLSISSGIVPDDLVDSDADSGSISRTSDSNTDEKASGELVSDWEDLDDNDLQNQFGLCSNPDEVSQHSSYKTVAEGVEVMQCNQPVVDPVNEGSHTCDEDTLVNSHDDGTYGEVQNEPDGVGVQLETPSILKKLKELFVLRQQAALSSQDFVYSSPDGYVPEETQVNANLSSQDESVPEETLLNANLSSQDESVPEETLLNANLCSQDESASGETQFSASLSSQDESVPEETPVNAIPSQVEFVPEEKQINANLSCQDQHVPEETQANASLSTPDHYVPEETQAKSSLRLSNKPVCGTVEDNTCVQQHHGNAELLRIITDLTKKADALEKNQIKSDQEIISLKEVIKDSGRKVQQLEYRIDELQFKFDSSLSLQGSMCDNLDRPSIFLIGGYNGVTWLSSLDSFSPKKDILVPLTSMGSARSYASVAAMEGCIFVFGGGDGSSWYNTAECYNTRSNEWMICPCLNHEKGSLAGVSLDGKIYAMGGGDGSQTYSEVEMFDPFLGKWICSPSMLQPRFALAGAESSGVIYATGGYDGHKYLQSAERYDPREGFWVRLPSMNARRGCHAVAVLDDVLYAIGGYDGVSMVSSVEIFDPRLNAWKMGDPMSSPRGYASAVTLDDSLFVLGGLRSNEQILDTVEVYNVSSGWTVPGFSSIGKRSFASAIVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3KE26</accession>
    <name>A0A0Q3KE26_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g68843v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3KE26"/>
    <dbReference type="EnsemblPlants" id="KQK22692">
        <property type="protein sequence ID" value="KQK22692"/>
        <property type="gene ID" value="BRADI_1g68843v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22692">
        <property type="protein sequence ID" value="KQK22692"/>
        <property type="gene ID" value="BRADI_1g68843v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3KE26"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="84" mass="9362" checksum="F623626E0CF859CC" modified="2016-01-20" version="1">MPVSRSAKFLDWELAMGRLGPGAFCTAPEHRKQSWSRRLTEEAGGNCSICTDLGFYCCKFFLPILTLKALLLLFTAQVLTTSMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LQ82</accession>
    <name>A0A0Q3LQ82_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g12790v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LQ82"/>
    <dbReference type="STRING" id="15368.A0A0Q3LQ82"/>
    <dbReference type="EnsemblPlants" id="KQJ94717">
        <property type="protein sequence ID" value="KQJ94717"/>
        <property type="gene ID" value="BRADI_3g12790v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ94717">
        <property type="protein sequence ID" value="KQJ94717"/>
        <property type="gene ID" value="BRADI_3g12790v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LQ82"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LQ82">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10538">
        <property type="entry name" value="SET_SETDB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.850">
        <property type="entry name" value="Histone-lysine N methyltransferase , C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025776">
        <property type="entry name" value="SUVR4/1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043017">
        <property type="entry name" value="WIYLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018848">
        <property type="entry name" value="WIYLD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46450:SF11">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SUVR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46450">
        <property type="entry name" value="INACTIVE HISTONE-LYSINE N-METHYLTRANSFERASE SUVR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10440">
        <property type="entry name" value="WIYLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51580">
        <property type="entry name" value="SAM_MT43_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="564"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="177"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="321"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ94717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="734" mass="81227" checksum="015831349F4ADAF0" modified="2016-01-20" version="1">MPPDSPPPPPRLPRAEDEAISPRTILDSRWRLGFPFASSYLVFMGLAGLSRPVSVLMGLLMEACLSLLFYKPFIFPSVPIPSASATTTDAGRDASSSSPPHRKRWDCLLLLLNNNMGSNNERFKKAFEAMKPLGISKKQMKPILRHLYNLFDKNWEPIEEENYRILADAILDAQNERPMPAAPNDETNGEPTPHSSTQLRPDDQRPSASASCYYTDVNDNETPLIKRSRRSRMSSADFVHEQPTQPIAIEGMSSQPASLQPNEPIRALAVVRQADDHGDPSSIVACKRDKGLLLESPEDVLFLNGPEPEIDLPAAPVAKHLNTSSSESGAVHSEAQSKGGSLQKEAVESDAPSVKPIACKHAVDGNSNAAVGFTVSESSEPTFQNCIVSCQPELPLSKQRPLHDVADISKGEERVSIPIVNEFGSESCPPLFYYIRKNLVFQSAYVHTSLARIGNEDCCTDCSGDCLLAPLPCSCSRLTGGEFAYTPEGLVKGEFLDECIAVNHFPEKHNKFYCKACPLERSKNNALPDPCKGHLARKFIKECWSKCGCGMQCGNRVVQCGITCNLQVFFTKEGKGWGLRTLDELPKGAFICEYVGEILTNTELHKRTVQNEKRSKHVHQVLLDANWGSEGVSRDEEALCLDPTFYGNVGRFVNHRCYDSNLVVIPVEVETPDRHYYHVAFFAARKIKAFEELTWDYGIDFDGTDIAFECMCGSKYCRNPKNSRKRARAATSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NC44</accession>
    <name>A0A0Q3NC44_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g16652v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NC44"/>
    <dbReference type="EnsemblPlants" id="KQK14494">
        <property type="protein sequence ID" value="KQK14494"/>
        <property type="gene ID" value="BRADI_1g16652v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14494">
        <property type="protein sequence ID" value="KQK14494"/>
        <property type="gene ID" value="BRADI_1g16652v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NC44"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110:SF76">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33110">
        <property type="entry name" value="F-BOX/KELCH-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK14494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="502" mass="55131" checksum="B98BF53F97C177DB" modified="2016-01-20" version="1">MASSSSCSDDSWSQLPQDILVLFVARLPFPGDRARFRAVCRCWRSAVRHHVPPSSSSSSFSPWVLRPDGYFFTPSDDGTWHRLPANSFFPDDGGTVGGGSTDAWIALLRRNSADCCFLLQNPFSGATVRLPELDAVLGGDSTTDVRKVVVRDDDGEQEELLVTVMTDNGNHPLVLCRPGARHYWSPEPWEVPYLYIVDVAFLGDGKLYAITKAEDLVSLDLAYSDGGSPVVVNGACLIRQPLGYDDYDAWTDDDAVEPPHHDHEEAQVVVVAEQLEVDDDDHDEQQHAEEPVTDDDDDDDDVTREGLDYAYDELGDLIITVRYLVAEPRSEKLFVLLLKLHPEGRGLEADVGAGSWLPVTSAARLGGQTLFISKRFSKFAPSDGSGDQVQDGCVYFVDTGEVFDVGSQTVSLTRYSFAAAVPVPLSAAGQFQHRIGAHPAATSSLLAIKVFGDLPRPWIRTTKRCSPRFLTRKSPLPMMLPPAANRNINSSSRRFLSSSPKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NWZ0</accession>
    <name>A0A0Q3NWZ0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100822786</name>
        <name evidence="12" type="ORF">BRADI_1g65170v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558129.1">
        <property type="nucleotide sequence ID" value="XM_003558081.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NWZ0"/>
    <dbReference type="STRING" id="15368.A0A0Q3NWZ0"/>
    <dbReference type="EnsemblPlants" id="KQK22094">
        <property type="protein sequence ID" value="KQK22094"/>
        <property type="gene ID" value="BRADI_1g65170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822786"/>
    <dbReference type="Gramene" id="KQK22094">
        <property type="protein sequence ID" value="KQK22094"/>
        <property type="gene ID" value="BRADI_1g65170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822786"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3NWZ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048250">
        <property type="term" value="P:iron import into the mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000308">
        <property type="entry name" value="Mitochondrial substrate carrier family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758:SF11">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758">
        <property type="entry name" value="MITOFERRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="102"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="198"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="291"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK22094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="41926" checksum="F535514FACB282A0" modified="2016-01-20" version="1">MPQDRFDHRGTNSSFPSPPPKWRHLLQISHPPLPLLYSTPESSSPSPTPLKTLTLPGPLAMAADYRTPDRLLPAAAEEPSQDPPKPVLSVAGPAAAATHDGLRFWQYMLAGSVAGLVEHTAMFPVDTLKTHMQAGAPPCRPTLSLGAALRAAVSGEGGALALYRGLPAMALGAGPAHAVYFSVYEFAKSRLSDRLGPNNPVAHASSGVLATVASDAVFTPMDTVKQRLQLTSSPYTGVSHCVRTVFRDEGLRAFFVSYRTTVLMNAPYTAVHFSTYEAAKRMLGDLAADEESLAVHATAGAAAGALAAAITTPLDVVKTQLQCQGVCGCERFSSSSIGDVFRTIIKRDGYIGLMRGWKPRMLFHAPAAAICWSTYEASKSFFERFNEKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3P3A1</accession>
    <name>A0A0Q3P3A1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100841502</name>
        <name evidence="2" type="ORF">BRADI_5g13737v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
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            <strain evidence="2 3">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
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                <person name="Barry K."/>
                <person name="Shu S."/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF13">
        <property type="entry name" value="AGMATINE HYDROXYCINNAMOYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK13770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="463" mass="50001" checksum="0DD2970C0F38FF88" modified="2016-01-20" version="1">MKVKIESSKIVKPLYDDGTAAPPPGAGGEWSPLSVFDKVTYTEHVALIYAFRPPSPPNSDLELGLAKALAVYREMAGRLGDGPDGLGRSVHLNDAGARFVEASVDGPLAASLPLLVRPSPVLKRLHPSSIDDDDGRPAEELVLVQLTRFSCGSLVLGFAGHHQIADGQSAGNFLVAWGLATRRISPVAPAPVCDRATRFAPRDPPLVAFPHRETEYKLPPLQAKKKHETDEDDDEEEFAETAAHEKVKVHKMHLSKEFVARLKARAAAASSSRGYTTFQSVVAHLWRAITAARGLGAGVGTKVRISVSGRTRMRPPVPRDGYFGNLVLWAFPRADAGDLVARPLGHAAELVRAAVAAVDDAYFRSFVDFASSGVVEAEGLVPTADSDQTALCPNLELDTWLGINFCDLDFGGGCPFYFMPTYFPMEGSLFLAPSFLGDGGMEAYVSLFDNHLEEFKKICYKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGN0</accession>
    <name>A0A2K2CGN0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g11249v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CGN0"/>
    <dbReference type="EnsemblPlants" id="PNT61177">
        <property type="protein sequence ID" value="PNT61177"/>
        <property type="gene ID" value="BRADI_5g11249v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61177">
        <property type="protein sequence ID" value="PNT61177"/>
        <property type="gene ID" value="BRADI_5g11249v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CGN0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT61177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="273" mass="30481" checksum="4F8B03DFA20993D9" modified="2018-03-28" version="1">MGAGKGSGLAAERGDGEEDGARAGCGGVRRGAAAGLGQDVEAVMRGSGGGERPFSSPTDFGPHHHPPPIPHPPAPCSLSTPAASRAAAGPPPRAFAAAPRLAWLRAPSLQPPTLPPPQLRRRARPAPRAALASPRKAMESSLSEIRSPAHRLRARKPRRRAPAHRRAKRRPRRTFPRRRARQTRKRRRQVPTRRGGQRRPGRTRALRRWPARTRRRASAPRARTRPWSPPYGRPPHRLQARKSRRRAPKHRGGQLFPRRTRVQWRVRQRPLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHI1</accession>
    <name>A0A2K2CHI1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g15785v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHI1"/>
    <dbReference type="EnsemblPlants" id="PNT61485">
        <property type="protein sequence ID" value="PNT61485"/>
        <property type="gene ID" value="BRADI_5g15785v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61485">
        <property type="protein sequence ID" value="PNT61485"/>
        <property type="gene ID" value="BRADI_5g15785v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CHI1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT61485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="75" mass="8333" checksum="2A50AD146BC711B7" modified="2018-03-28" version="1">MKIGGDRQRGAAGTRNSGVILRWWCASVRLGKQKWLLWRNTSWCGVKSWVHDSTHQGSNPGAHNYALGVSPTVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CKB1</accession>
    <name>A0A2K2CKB1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">At1g61320/AtMIF1 LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g03905v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CKB1"/>
    <dbReference type="FunCoup" id="A0A2K2CKB1">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CKB1"/>
    <dbReference type="EnsemblPlants" id="PNT62473">
        <property type="protein sequence ID" value="PNT62473"/>
        <property type="gene ID" value="BRADI_4g03905v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62473">
        <property type="protein sequence ID" value="PNT62473"/>
        <property type="gene ID" value="BRADI_4g03905v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CKB1"/>
    <dbReference type="OrthoDB" id="600819at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF71">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="At1g61320/AtMIF1 LRR" evidence="2">
        <location>
            <begin position="175"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT62473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="504" mass="55868" checksum="7EE2BFAA7EC46C48" modified="2018-03-28" version="1" fragment="single">GLVVSLDERKASAGQKDCVATGSIASQGKRKGSPCQQEDSSQSGEEKGYPGPELPEAYIINFCLSHILIIGTCDCDSLKQSERHIHVGFHHLSLLPQLQPNAPSISPLQWDIWIQIHSLMPMRDAARATCVSRASLCSWRSHPNLTFSMKTLGLKGRVSENVEIAKDYATKLDHILKKHSGVGVKTFQLLDAPSCSVKDHYYLDSWLQMAVKPGIEELAISPSDGNGNSIRSLYLDCCAFQPTVGFGCLRSLTSLYLGMVRITGKGLGWLLSNSFALERFTLRCCSEIYRLKIPCHLRRLSYLEVLYCRELKVIESEAPNLSNMRVAAGFQLQLLLGGAESQLGSSMPNLETLSIESTKETVNRPTVSGRFLHLKYLMIYVTVDNSPRYDYVLLASLIDASPSLATLVLDVSQRRMEHVSIFEDPSGLRQLPEQTHAKLQNVQFSGFSSAKMIELTRHILAVSTSLKKLDHIVEALRAVLAIDMYIRPKVPSTVKLCVRAMPCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMB0</accession>
    <name>A0A2K2CMB0_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BRADI_4g12255v3</name>
    </gene>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="21"/>
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    </feature>
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            <begin position="66"/>
            <end position="107"/>
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        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="205" mass="22620" checksum="CFD78E61E75F8477" modified="2018-03-28" version="1">MQEAKKQKPSSEVGKFSKATGNTLAGEHSCVDLIEKPALVTATIGEGFAGGLTQQEQDKILATQAAEQDQQKLTELPEDVDELGEELEGSQERVQLSEEELIDSQDSFETKVDNILGNKGKISDVKEKAKENQKELEKMDKKLMPTRKCERLKGQEDADRTKLAMQRAEQKNSIPGATNVFSESELCDCEGCLNSNYYQGGSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CNL6</accession>
    <name>A0A2K2CNL6_BRADI</name>
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        <recommendedName>
            <fullName evidence="2">PB1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g18369v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CNL6"/>
    <dbReference type="EnsemblPlants" id="PNT63620">
        <property type="protein sequence ID" value="PNT63620"/>
        <property type="gene ID" value="BRADI_4g18369v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63620">
        <property type="protein sequence ID" value="PNT63620"/>
        <property type="gene ID" value="BRADI_4g18369v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CNL6"/>
    <dbReference type="OrthoDB" id="695246at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058594">
        <property type="entry name" value="PB1-like_dom_pln"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26130">
        <property type="entry name" value="PB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1-like" evidence="2">
        <location>
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            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT63620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="290" mass="32536" checksum="DFB90A4B6D678B52" modified="2018-03-28" version="1">MAPLRRLEGNPPPVYEDWDRRFLFSLCINHGGFFVGSGTNRSYVDGRKIWYDFIDGEKWSLADVETIVEEIGYEMAGRVKAYWLVPGKEANNLNGLVQISSAAVTDLPKVFSPKKTTYLENRSEALPAFYKVANPVIIQSNSESSDSESGGEEDFIDSDYEISDGDDDLGEDVIDEDEASDKGKGKAVQQELSDNEYDAEDDMPDEGDHLRAPGSDDEGAVYNFKSFNEEDTHNPVFKYSCKMRVDIKLPINDTQRISASCDDGCPWRMWASYDSRTKCMALAVILNHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CNZ6</accession>
    <name>A0A2K2CNZ6_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g20755v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CNZ6"/>
    <dbReference type="EnsemblPlants" id="PNT63767">
        <property type="protein sequence ID" value="PNT63767"/>
        <property type="gene ID" value="BRADI_4g20755v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63767">
        <property type="protein sequence ID" value="PNT63767"/>
        <property type="gene ID" value="BRADI_4g20755v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CNZ6"/>
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        <property type="component" value="Chromosome 4"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT63767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="108" mass="12159" checksum="1E3413177135F358" modified="2018-03-28" version="1">MAIHAQEAKKDRGLVVAMARRRCCSRVPGWQDPAPRSTAAKQLGRRREGGSSKQRERRKTEPTRTPSCRTRGEQRQRRASCPLVGGLLVSARLRDLATCYTRKEQSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CPZ2</accession>
    <name>A0A2K2CPZ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
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    <gene>
        <name evidence="10" type="ORF">BRADI_4g24006v3</name>
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    <organism evidence="10">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="PNT64063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CPZ2"/>
    <dbReference type="STRING" id="15368.A0A2K2CPZ2"/>
    <dbReference type="EnsemblPlants" id="PNT64063">
        <property type="protein sequence ID" value="PNT64063"/>
        <property type="gene ID" value="BRADI_4g24006v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64063">
        <property type="protein sequence ID" value="PNT64063"/>
        <property type="gene ID" value="BRADI_4g24006v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CPZ2"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CPZ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF492">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE PTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="132"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="314"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="18"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="19"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="PNT64063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="402" mass="44856" checksum="DF47C85643B0D33E" modified="2018-03-28" version="1">MSFSFREKLWSDRRLYLYSANPPGSTTATPTASPASPSPSPPPAPRPKRRRETTPDPAAATGNSPLRGGRGGRAAAASSVRRRSATPPPPAGFDPLDPDAVPPPRTLTRDQVKHCKNALKVFEKKLKDPAAISQEFRALQDIRKQLLSTQKFTVAQNPANGERNRYTDVLPFDETRIKLQSSTGNETASNDYINASLIKRNDGSDQTKFISTQGPLVNTFEDFWQMVFENSCPVIVMLTKFDSVKCDEYLPLRKGQGAYGKFNIKITKTKHDGQLLLRMVKVHSNESDRVHTVLHIQHSTWPDHGVPNDSKTVRDILKRLHSVPKEHPIVAHCSAGIGRTGAYITIHNTIERILLGEQTAMDLAETVRNFRSQRPGMVQTEDQFKFCYHAVVDELKDLVTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQF3</accession>
    <name>A0A2K2CQF3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC112272439</name>
        <name evidence="9" type="ORF">BRADI_4g26621v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318952.1">
        <property type="nucleotide sequence ID" value="XM_024463184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQF3"/>
    <dbReference type="STRING" id="15368.A0A2K2CQF3"/>
    <dbReference type="EnsemblPlants" id="PNT64249">
        <property type="protein sequence ID" value="PNT64249"/>
        <property type="gene ID" value="BRADI_4g26621v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112272439"/>
    <dbReference type="Gramene" id="PNT64249">
        <property type="protein sequence ID" value="PNT64249"/>
        <property type="gene ID" value="BRADI_4g26621v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2015071at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707">
        <property type="entry name" value="C2 CALCIUM/LIPID-BINDING PLANT PHOSPHORIBOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707:SF43">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="787"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="38"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="375"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="487"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="560"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="514"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="576"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="641"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="670"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="688"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT64249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="816" mass="89617" checksum="0490A94917BEAC06" modified="2018-03-28" version="1">MADHARELEALERILSDDAGAEPTRLAYGLMKSITNDFSPSREIGSGGFGVVYLGILRKGTVAVKKLTMSPTFSDDLFLDEVRCLMRTKHKNIVRFLGYCSDTQGELIEHNGRKIMAEVRQRLLCFEYVPNGSLQRYLKEKCHGNGWKIRYQFMKGICQGLHYLHKERINHLDLKPENVLLDAYMEPKISDFGLSRWFDEGQSRIVTINTPGTRGYIAPEIIDKGEISFKSDIFSLGIIIIKLLTGSDHHEFENWHESIDVEGPQVKSCIEIARTCVEADQHNRPCISEIIDKLNEMENAEESRDGTGSSMVSPSSSKEVIPPSTMCRMDKQDCEYAKMDTCLADGMGHSKHDSGVSKDTKTVQLLGQRRVEDNDGSNFLVHDKDLYNGPKMMTNQKARSGDEKGPNGGSGPSNQGKMSGMLLTPPAMMRPNMMPGVGMLQAAAAAGNPIALKNIQMMQQQMMMNNGGTGYPRMGYGMQGMYPMPPNMMQHTAPTKVDPVKFKNPEPEGGGGSESHDLDNEESKDSGSKAHKSGGGKQDTKMYPMGPQKIQMKLSTPLMDAPAASKDPKSIKFNLPEDEFDDDDDEFSDFDDDDDDFDDDGLDDDDPNAKQVGVPPHAATTRGSDKKGGSENEIPAQIKGNNGGKKGGGGGKNVGGAQPNNIKGQEKKGTGTGGLATGGLMGGMLPPQLLPPRPLPPQQQQAMLRPTMMGSSGGFPGMGQMGYRPHMENGVHGMLPPPPPPPTGFYQSGMPPYDREREILHIAAAAGNSMAQLQHRALAQQQQQQHMAFYLFLFMFLFLFTIVIIIIVIFTFKARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CT49</accession>
    <name>A0A2K2CT49_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF868 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100821642</name>
        <name evidence="2" type="ORF">BRADI_4g38410v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758018.1">
        <property type="nucleotide sequence ID" value="XM_014902532.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CT49"/>
    <dbReference type="STRING" id="15368.A0A2K2CT49"/>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Wang M."/>
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</entry>
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                <person name="Tice H."/>
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                <person name="Bruce D."/>
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                <person name="Shu S."/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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            <strain evidence="2 3">Bd21</strain>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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    </evidence>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CZ50"/>
    <dbReference type="EnsemblPlants" id="PNT67304">
        <property type="protein sequence ID" value="PNT67304"/>
        <property type="gene ID" value="BRADI_3g23961v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67304">
        <property type="protein sequence ID" value="PNT67304"/>
        <property type="gene ID" value="BRADI_3g23961v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CZ50"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="123" mass="13333" checksum="6950657FFFA3A409" modified="2018-03-28" version="1">MRKSRKKGKTGPVRPTWTEPAEPARTAPEPDRAPARGHRQIFAAAASSSSSGDPRRPSRRRRRSRVSRPSPASCPPGALVRPPSRARPSSEPPRRRRAAPPRAAPRRPPSLRSPPSDVPAPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D2D8</accession>
    <name>A0A2K2D2D8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g40750v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D2D8"/>
    <dbReference type="FunCoup" id="A0A2K2D2D8">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68454">
        <property type="protein sequence ID" value="PNT68454"/>
        <property type="gene ID" value="BRADI_3g40750v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68454">
        <property type="protein sequence ID" value="PNT68454"/>
        <property type="gene ID" value="BRADI_3g40750v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D2D8"/>
    <dbReference type="OrthoDB" id="670584at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF430">
        <property type="entry name" value="MATH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="4">
        <location>
            <begin position="10"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT68454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35280" checksum="9DCDE8B80E1E5CEA" modified="2018-03-28" version="1">MRTTIVCSSVLKLTIDYEKAKQLPIGKYVESDVVSVGGHLWRIDCYPRGHSEDDKGEYLSLFLAPVNMSNTRSANAILDVFLMDKDGKPSATDARRLLHAFEVNSDGYSWGWPKFVKGTNLEKDYVAEGHITIVCTIMVTDERPIPVPPSDIGTHLGSLLDHNDRADVTFIVEDQTFHAHRAFLAARSRRHDITPATFKCMLHFIYSDDLPGDDELGCSSVEMLQDLLAAADRYALDRLKLMCAQKLWEHLSIDTVATTLACAETYNCPELKNKCFNFFAVEKNFKKAVFTDGFATLLQKFPSITDELKSKVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D5B4</accession>
    <name>A0A2K2D5B4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g56050v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D5B4"/>
    <dbReference type="STRING" id="15368.A0A2K2D5B4"/>
    <dbReference type="EnsemblPlants" id="PNT69469">
        <property type="protein sequence ID" value="PNT69469"/>
        <property type="gene ID" value="BRADI_3g56050v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69469">
        <property type="protein sequence ID" value="PNT69469"/>
        <property type="gene ID" value="BRADI_3g56050v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D5B4"/>
    <dbReference type="OrthoDB" id="692734at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044997">
        <property type="entry name" value="F-box_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32153">
        <property type="entry name" value="OJ000223_09.16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="7"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT69469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="442" mass="51117" checksum="2AC55E55384AAFF2" modified="2018-03-28" version="1">MQGDEGEDRLSKLPDDVLLSIVERLDIADAARTRILSRRWRQIPAMLSKVVIECRHRRSKLHRHDVGWPNSNLVEATRSILGSRTANLYTIDLLRLQFYLGDDSILIGKAVSNTMATQKVCLAEFTVMTKKDRTRCTHEEKLAYGRQLRSFIYACPNAFSGLTRLKLENLISETDFPKIFSTCTRLEFLRLHGCGMGMFSLLEVEHPLLSELEIADCSFEEVYLKWLPKITLLTFSDWISQHDPVTFGYVPLLQSMSINNTGLRHHKMLKLSELLGNTTISDLHLNFQSEKIWVEPEGPKELYQVRDHLCEMVWDKAERKMYGFSEEKKDKGLEWDASASDFKHQKLAVLRIFGFQTEDKFVSHIRRVMEAAVNLENIYLHNKTVCKRCQPWVEKPSRYPYTLKQKTSLRNKINHGIASLKFTSRIYQPASVNAAVCFRAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D8U5</accession>
    <name>A0A2K2D8U5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g16305v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D8U5"/>
    <dbReference type="EnsemblPlants" id="PNT70693">
        <property type="protein sequence ID" value="PNT70693"/>
        <property type="gene ID" value="BRADI_2g16305v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70693">
        <property type="protein sequence ID" value="PNT70693"/>
        <property type="gene ID" value="BRADI_2g16305v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D8U5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT70693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="250" mass="27304" checksum="8391812E1332FCBF" modified="2018-03-28" version="1">MHHPDRLPSPFHRNKNLYCYLTCSCVRGGSYVVPTSQKLRLYATVRTPICFFSFVFSCLAQPAAHAPPLLLPCAPLPPPTCLSSRSSSARTATAPISLCQRCLLLLLAHTRPDPPPPPRTRPALIHLLLARGRGARGGGRDARRGCRPPGRDPPPPHCAAAVGEPSSAAPRPRDATCSPPSQRIECPMPPAEARVLETSAMTASKWEASILCGKVMSYGNLRRVQMLWFDTEQKKEVSFPLKMRVSIGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DAV0</accession>
    <name>A0A2K2DAV0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_2g27172v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DAV0"/>
    <dbReference type="EnsemblPlants" id="PNT71414">
        <property type="protein sequence ID" value="PNT71414"/>
        <property type="gene ID" value="BRADI_2g27172v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71414">
        <property type="protein sequence ID" value="PNT71414"/>
        <property type="gene ID" value="BRADI_2g27172v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DAV0"/>
    <dbReference type="OrthoDB" id="685351at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF19">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="6">
        <location>
            <begin position="3"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT71414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="181" mass="20705" checksum="22B7BD92DB12D561" modified="2018-03-28" version="1" fragment="single">ILVWNVRGLNCRARRLGVDTTGASIVFLQETKLSVVTPTLVMEVLGADFDDYFCLPATDTRGGILIPWDSRMIQLGSPHITDNCVTGLVTPSQGPQWWLSCVYGSQEDVDKIAFLDELRDVRSTQSDPWVVCGDFNLIYQEEDKSNSNLNRRMMGRFCRFLNDCELKEIYLHGRRYTWSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DBX1</accession>
    <name>A0A2K2DBX1_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g35392v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DBX1"/>
    <dbReference type="EnsemblPlants" id="PNT71775">
        <property type="protein sequence ID" value="PNT71775"/>
        <property type="gene ID" value="BRADI_2g35392v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71775">
        <property type="protein sequence ID" value="PNT71775"/>
        <property type="gene ID" value="BRADI_2g35392v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DBX1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT71775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="149" mass="17112" checksum="2453649152BCB0ED" modified="2018-03-28" version="1">MGQVERGKGGEGPIRHRRGWRWRWRGRQRARASSWSPRFSCCWRPRRRRTSGWTSWTATGRTISPGGPAAAGTAGSGLVHASPAGHRGRGSKANMLFVHDACNRIHDRRHVRPRYIGKPKLHFFCFLSWRIEGSIRLCLVNYLLYDVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DFW0</accession>
    <name>A0A2K2DFW0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g54613v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DFW0"/>
    <dbReference type="EnsemblPlants" id="PNT73163">
        <property type="protein sequence ID" value="PNT73163"/>
        <property type="gene ID" value="BRADI_2g54613v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73163">
        <property type="protein sequence ID" value="PNT73163"/>
        <property type="gene ID" value="BRADI_2g54613v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DFW0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT73163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="68" mass="8022" checksum="708AE917C9D97D04" modified="2018-03-28" version="1">MYNEHFSGYLRVMCYVQENTLLAYWNHLPLVMDLPLICIWLNMNTPSTLVCTVKNCERGVFVRKDARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DSP5</accession>
    <name>A0A2K2DSP5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g60810v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DSP5"/>
    <dbReference type="FunCoup" id="A0A2K2DSP5">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DSP5"/>
    <dbReference type="ESTHER" id="bradi-a0a2k2dsp5">
        <property type="family name" value="TGL-GrainShapeFactor"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77301">
        <property type="protein sequence ID" value="PNT77301"/>
        <property type="gene ID" value="BRADI_1g60810v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77301">
        <property type="protein sequence ID" value="PNT77301"/>
        <property type="gene ID" value="BRADI_1g60810v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DSP5"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DSP5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052370">
        <property type="entry name" value="Meta-cleavage_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139:SF68">
        <property type="entry name" value="ALPHA_BETA FOLD FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="77"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT77301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="336" mass="37052" checksum="EB70CF974F279CE8" modified="2018-03-28" version="1">MAPFPSQSQEAPQPPPAAAKRKRPCLSFTVARDRCLRRRFHSAGLRPFSIRLPSSAGSGTGTTVSLWAPPQPARRAVLLLHGFGASATWQWAPYLRRLIAAGFDPIVPDLLFFGDSASPAPDRSETFQARAVKAAMDAIGVRRFAVVGVSYGGFVAHRMAAMYPEAVERAVLVCAGVCLEESDLSVGLFPVAGVEEAAELLIPRRPSDVRRLVRLTFVRPPPIMPSCFLKDYINVMGSDHIEEKTELLRALINDRQLSDLPKISQPTLIIWGEQDKVFPLELAHRLNRHLDGNSRLVVIKRAGHAVNLEKDKEVCANIIEHLQEPVSCDDSSWEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLF7</accession>
    <name>I1GLF7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100823138</name>
        <name evidence="11" type="ORF">BRADI_1g03410v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563440.1">
        <property type="nucleotide sequence ID" value="XM_003563392.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLF7"/>
    <dbReference type="STRING" id="15368.I1GLF7"/>
    <dbReference type="EnsemblPlants" id="KQK12388">
        <property type="protein sequence ID" value="KQK12388"/>
        <property type="gene ID" value="BRADI_1g03410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823138"/>
    <dbReference type="Gramene" id="KQK12388">
        <property type="protein sequence ID" value="KQK12388"/>
        <property type="gene ID" value="BRADI_1g03410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823138"/>
    <dbReference type="eggNOG" id="KOG3480">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162151_3_0_1"/>
    <dbReference type="OMA" id="VGENMQK"/>
    <dbReference type="OrthoDB" id="274922at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000007">
        <property type="entry name" value="Mitochondrial import inner membrane translocase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038:SF16">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="10">
        <location>
            <begin position="16"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK12388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="83" mass="9633" checksum="9D1DAF94DE615490" modified="2012-06-13" version="1">MASKGGPSNLEKEQMFGMAEKEMEYRVDLFNRLTHSCFEKCIEKRHKESELNMGENSCIDRCVSKYWQVTNLVGQMLGNKPQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMQ5</accession>
    <name>I1GMQ5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100841880</name>
        <name evidence="5" type="ORF">BRADI_1g06917v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558522.1">
        <property type="nucleotide sequence ID" value="XM_003558474.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMQ5"/>
    <dbReference type="FunCoup" id="I1GMQ5">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK12930">
        <property type="protein sequence ID" value="KQK12930"/>
        <property type="gene ID" value="BRADI_1g06917v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841880"/>
    <dbReference type="Gramene" id="KQK12930">
        <property type="protein sequence ID" value="KQK12930"/>
        <property type="gene ID" value="BRADI_1g06917v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841880"/>
    <dbReference type="eggNOG" id="ENOG502QSM6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="RNWIPFD"/>
    <dbReference type="OrthoDB" id="411524at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GMQ5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000180">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050748">
        <property type="entry name" value="Glycosyltrans_8_dom-fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 8 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778:SF47">
        <property type="entry name" value="LIPOPOLYSACCHARIDE 1,3-GALACTOSYLTRANSFERASE"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK12930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="362" mass="40078" checksum="EC2B18B4177A54C5" modified="2012-06-13" version="1">MEGLQRRPAAAAANARAQQPPPAIHCDVEPAPRPWPGMQMLAIAAVLVLGGLQFLPATHFRHPADPGRNWVPFDPTRHPLDISGRIEIFSWISCLDLRTLAVLTNSTLSSSSEPHDVYFNFLIPEGGNDKLPFYKIKSVLPDSNITVTSQKQIKDKLNVATPEGNFFWSFHNELSSIIIATTQPSKKRYVYISADSVIKGKIEELARIDLGIYAIGAVEDCSKRVGDYTNMDVLNAVHRTAPTGLASTEPYNKDTCLLDIDVLVVEPRNLKRNTIDAIKVWVTGLSLANPRDGIQLAITLAFYDNYLKLPSSWKRGNANADILHYDGPKNVCSADGRQHQEQGSGETWRQYLSEKSDAMLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1GNZ2</accession>
    <name>I1GNZ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 9">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100833535</name>
        <name evidence="13" type="ORF">BRADI_1g10650v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="4 9"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK13515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KQK13516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KQK13517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560604.1">
        <property type="nucleotide sequence ID" value="XM_003560556.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230827.1">
        <property type="nucleotide sequence ID" value="XM_010232525.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752143.1">
        <property type="nucleotide sequence ID" value="XM_014896657.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GNZ2"/>
    <dbReference type="STRING" id="15368.I1GNZ2"/>
    <dbReference type="EnsemblPlants" id="KQK13514">
        <property type="protein sequence ID" value="KQK13514"/>
        <property type="gene ID" value="BRADI_1g10650v3"/>
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    <dbReference type="EnsemblPlants" id="KQK13515">
        <property type="protein sequence ID" value="KQK13515"/>
        <property type="gene ID" value="BRADI_1g10650v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK13516"/>
        <property type="gene ID" value="BRADI_1g10650v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK13517"/>
        <property type="gene ID" value="BRADI_1g10650v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74230">
        <property type="protein sequence ID" value="PNT74230"/>
        <property type="gene ID" value="BRADI_1g10650v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74231">
        <property type="protein sequence ID" value="PNT74231"/>
        <property type="gene ID" value="BRADI_1g10650v3"/>
    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="KQK13515"/>
        <property type="gene ID" value="BRADI_1g10650v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13516">
        <property type="protein sequence ID" value="KQK13516"/>
        <property type="gene ID" value="BRADI_1g10650v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK13517"/>
        <property type="gene ID" value="BRADI_1g10650v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74230">
        <property type="protein sequence ID" value="PNT74230"/>
        <property type="gene ID" value="BRADI_1g10650v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74231">
        <property type="protein sequence ID" value="PNT74231"/>
        <property type="gene ID" value="BRADI_1g10650v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833535"/>
    <dbReference type="eggNOG" id="KOG2666">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_7_0_1"/>
    <dbReference type="OMA" id="CQDKTLN"/>
    <dbReference type="OrthoDB" id="5059218at2759"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000001">
        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000089">
        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028356">
        <property type="entry name" value="UDPglc_DH_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374:SF3">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374">
        <property type="entry name" value="UDP-GLUCOSE DEHYDROGENASE/UDP-MANNAC DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500133">
        <property type="entry name" value="UDPglc_DH_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="UDP-glucose/GDP-mannose dehydrogenase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0520">NAD</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="328"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="127"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="272"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500133-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR500133-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="PNT74231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="481" mass="52929" checksum="5A2AFE9D070CC6F7" modified="2012-06-13" version="1">MVKICCIGAGYVGGPTMAVIAVKCPAIEVVVVDISKPRIDAWNSDHLPIYEPGLDEVVKECRGRNLFFSTEVEKHVAEADIIFVSVNTPTKTRGLGAGKAADLTYWESAARMIADVSKSDKIVVEKSTVPVKTAEAIEKILTHNSKGINYQILSNPEFLAEGTAIEDLFKPDRVLIGGRETPEGRKAVQALKEVYAHWVPEDQIITTNLWSAELSKLAANAFLAQRISSVNAMSALCEATGANVSEVSYAVGKDSRIGPKFLNASVGFGGSCFQKDILNLVYICECNGLPEVASYWKQVIKINDYQKSRFVNRVVSSMFNTVSGKKIAILGFAFKKDTGDTRETPAIDVCKGLLGDKAQVSIYDPQVTEEQIQWDLAMNKFDWDHPTHLQPTSPTAMKQVSVVRGDAYEATKGAHGICILTEWEEFKTLDYQKIFDNMQKPAFVFDGRNVVDAEKLREIGFIVYSIGKPLDAWLKDMPAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQ40</accession>
    <name>I1GQ40_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100831920</name>
        <name evidence="8" type="ORF">BRADI_1g14030v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232278.1">
        <property type="nucleotide sequence ID" value="XM_010233976.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232282.1">
        <property type="nucleotide sequence ID" value="XM_010233980.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232286.1">
        <property type="nucleotide sequence ID" value="XM_010233984.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318810.1">
        <property type="nucleotide sequence ID" value="XM_024463042.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318814.1">
        <property type="nucleotide sequence ID" value="XM_024463046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQ40"/>
    <dbReference type="FunCoup" id="I1GQ40">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK14039">
        <property type="protein sequence ID" value="KQK14039"/>
        <property type="gene ID" value="BRADI_1g14030v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK14041">
        <property type="protein sequence ID" value="KQK14041"/>
        <property type="gene ID" value="BRADI_1g14030v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK14042">
        <property type="protein sequence ID" value="KQK14042"/>
        <property type="gene ID" value="BRADI_1g14030v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK14043">
        <property type="protein sequence ID" value="KQK14043"/>
        <property type="gene ID" value="BRADI_1g14030v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK14044">
        <property type="protein sequence ID" value="KQK14044"/>
        <property type="gene ID" value="BRADI_1g14030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831920"/>
    <dbReference type="Gramene" id="KQK14039">
        <property type="protein sequence ID" value="KQK14039"/>
        <property type="gene ID" value="BRADI_1g14030v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14041">
        <property type="protein sequence ID" value="KQK14041"/>
        <property type="gene ID" value="BRADI_1g14030v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14042">
        <property type="protein sequence ID" value="KQK14042"/>
        <property type="gene ID" value="BRADI_1g14030v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14043">
        <property type="protein sequence ID" value="KQK14043"/>
        <property type="gene ID" value="BRADI_1g14030v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14044">
        <property type="protein sequence ID" value="KQK14044"/>
        <property type="gene ID" value="BRADI_1g14030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831920"/>
    <dbReference type="eggNOG" id="KOG0253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_0_1"/>
    <dbReference type="OMA" id="WDVVIYS"/>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GQ40">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000232">
        <property type="entry name" value="Organic cation/carnitine transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="22"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK14043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="481" mass="52698" checksum="572D2C24F6104194" modified="2012-06-13" version="1">MGTYTTDDALAAMGFGKFQALVLAYAGMGWVAEAMELNLLSFVGPLVRDEWEISAQDESLLSSVVFAGMLTGSCTWGFVSDKYGRRTALLFSALFTSGMGFLSALSPDYLCLLALRFLVGVGVGGGHVFSSWFLEFVPAQNRGTWMVLFSLFWTVGTILEASLAWVVILTLSWRWLLALTALPCFLLLPFFGTTPESPRFLCAQNRMSDATLVLERISMTNQSALPSGVLTYHPQSKVVHSTFVSETETDHFLPVTETERKDDDASSFKSGVVAALRKLLAPELLRSTLLLWFVYYANSFAYYGLVLLTTQLSDANRRCPSRLQNAQRQEDANVYKDTFFTSLAEIPGLILSAVLVEWLGRKATMWCMLFTCCAFLEPLVLHQNELLTTALLFGARACAMGSSTVICLYAPEVYPTSVRSTGVGIATSIGKIGGVICPLVAVGMLRSCHQMEAVLVFEVVLFLAGVACLLFPVETKGRQID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQW4</accession>
    <name>I1GQW4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC104583389</name>
        <name evidence="4" type="ORF">BRADI_1g16730v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233621.1">
        <property type="nucleotide sequence ID" value="XM_010235319.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQW4"/>
    <dbReference type="STRING" id="15368.I1GQW4"/>
    <dbReference type="EnsemblPlants" id="KQK14504">
        <property type="protein sequence ID" value="KQK14504"/>
        <property type="gene ID" value="BRADI_1g16730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583389"/>
    <dbReference type="Gramene" id="KQK14504">
        <property type="protein sequence ID" value="KQK14504"/>
        <property type="gene ID" value="BRADI_1g16730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583389"/>
    <dbReference type="eggNOG" id="ENOG502QQXS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024423_1_0_1"/>
    <dbReference type="OMA" id="EAWRSHE"/>
    <dbReference type="OrthoDB" id="10006218at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057192">
        <property type="entry name" value="DUF7870"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44843">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44843:SF14">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25276">
        <property type="entry name" value="DUF7870"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase type 11" evidence="2">
        <location>
            <begin position="136"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7870" evidence="3">
        <location>
            <begin position="271"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7870" evidence="3">
        <location>
            <begin position="408"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK14504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="55277" checksum="A393540845452239" modified="2012-06-13" version="1">MEPPPQQPASRKWSGKGILTRALLLGIAALALRLLYGAFLLVAVGGGGGWAVRPAAVVVGRRTNVEAAAGATPEAWRSREWREAVGFHAALLAGHLGGSHDDGNGLLLAPSSRAVCLGPAAQEAALALRELGVHGAVAVASKRRPPLAVAGDDRRLPFPAASVDFVLAARALDASKRPADLAAEAARILKPGAHLVVLTAGAADAYSLRSLQALLLPSLRLLRSREIDGPDGSTLRELVFQKLHVAPASSLSHDPVSNCTIGDHKLPLLTRAEPLILEEPLKPWLTLKRNLKNIKYLPELADISFKRRHVYVDVGARSYGSSIGSWFRKQYPKQNHTFEVFAIEADPAFHPEYATRKGVTLLPYAAWVKNETLNFEINGDPGKEEEAKANGRGMGRIRPIAGKKMSGEVRSVPAFDFAEWLKQTVSQQDYVVMKMDVEGTEFDLIPRLFDTGAICLVDELFLECHYNRWQKCCPGERSPKYQNTYEECLELFSSLRDSGVLVHQWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSR7</accession>
    <name>I1GSR7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100822514</name>
        <name evidence="12" type="ORF">BRADI_1g22680v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WRKY group I family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562765.1">
        <property type="nucleotide sequence ID" value="XM_003562717.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSR7"/>
    <dbReference type="EnsemblPlants" id="KQK15421">
        <property type="protein sequence ID" value="KQK15421"/>
        <property type="gene ID" value="BRADI_1g22680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822514"/>
    <dbReference type="Gramene" id="KQK15421">
        <property type="protein sequence ID" value="KQK15421"/>
        <property type="gene ID" value="BRADI_1g22680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822514"/>
    <dbReference type="eggNOG" id="ENOG502QTWW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012086_7_2_1"/>
    <dbReference type="OMA" id="RGQHTHE"/>
    <dbReference type="OrthoDB" id="2021103at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000006">
        <property type="entry name" value="WRKY transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000003">
        <property type="entry name" value="WRKY transcription factor 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF167">
        <property type="entry name" value="OS07G0596900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="WRKY" evidence="11">
        <location>
            <begin position="177"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY" evidence="11">
        <location>
            <begin position="336"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="283"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK15421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="447" mass="49031" checksum="BCDD3C00CE20F234" modified="2012-06-13" version="1">MAAEQWSGGGGGLWAPPSAAMESLFPDEPAPAAAVLGIFGAWGLQAQLHSLPPLCAAALLGYPQDNFDMFHAQDLAQLAAQVAQKAELEETHSGELNPKITPQIAYTKYSILDQAHNSSFSSATSAQTSQHVSSSVIAPSMWCIPTLPSHTECIKTESNRVSQVLQGASITLDRPADDGYNWRKYGQKAVKGGRYPRSYYKCTLNCPVRKNVEHSEDGKIIKIIYRGQHSHERPSKRYKDCGILLKESDDFNDTEDASTKSQLDCLGYDGKPVTSIGTMADYSLPMREGGDEKVSGTSDYRGEGDDETRTADEAVGDTDANERNAPGQKIIVSTTSDVDLLDDGYRWRKYGQKVVRGNPHPRSYYKCTYQGCDVKKHIERSSQEPHAVITTYEGKHVHDVPGSRNRSHAAGQPYCTEQTYSEQSSASFCSRSEKGKYSAVSLKHVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTV9</accession>
    <name>I1GTV9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100844346</name>
        <name evidence="2" type="ORF">BRADI_1g25990v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563010.1">
        <property type="nucleotide sequence ID" value="XM_003562962.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GTV9"/>
    <dbReference type="FunCoup" id="I1GTV9">
        <property type="interactions" value="1284"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GTV9"/>
    <dbReference type="EnsemblPlants" id="KQK15953">
        <property type="protein sequence ID" value="KQK15953"/>
        <property type="gene ID" value="BRADI_1g25990v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844346"/>
    <dbReference type="Gramene" id="KQK15953">
        <property type="protein sequence ID" value="KQK15953"/>
        <property type="gene ID" value="BRADI_1g25990v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844346"/>
    <dbReference type="eggNOG" id="ENOG502S5I4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092975_0_0_1"/>
    <dbReference type="OMA" id="SHAAWNI"/>
    <dbReference type="OrthoDB" id="779856at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GTV9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010089">
        <property type="term" value="P:xylem development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039280">
        <property type="entry name" value="VUP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33974:SF2">
        <property type="entry name" value="VASCULAR-RELATED UNKNOWN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33974">
        <property type="entry name" value="VASCULAR-RELATED UNKNOWN PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK15953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="193" mass="21168" checksum="EB504F72EAC64EC6" modified="2012-06-13" version="1">MDDLVSSPSFKSVLSCSEAEAQPEESSWTGYFVDFMMSEEEKKRQDASYCSYDDEEEEVDGSMISDAASLAPAAFPDKYKGLKQLKKKVFKALDHDDSLEDTASSPVNSPKVSALSQMEFSPKRRCNVSDLAKGAGIGNDHGREGTDCEEHADAIMEGVRFLDQSQRGITPCAELKDKGLCLVPLSMLLNYQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1GW34</accession>
    <name>I1GW34_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">4-hydroxy-4-methyl-2-oxoglutarate aldolase</fullName>
            <shortName evidence="2">HMG aldolase</shortName>
            <ecNumber evidence="2">4.1.1.112</ecNumber>
            <ecNumber evidence="2">4.1.3.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Oxaloacetate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836315</name>
        <name evidence="3" type="ORF">BRADI_1g32490v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate</text>
            <dbReference type="Rhea" id="RHEA:22748"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:58276"/>
            <dbReference type="EC" id="4.1.3.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>oxaloacetate + H(+) = pyruvate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15641"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="EC" id="4.1.1.112"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class II aldolase/RraA-like family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.112" evidence="2"/>
    <dbReference type="EC" id="4.1.3.17" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563400.1">
        <property type="nucleotide sequence ID" value="XM_003563352.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GW34"/>
    <dbReference type="EnsemblPlants" id="KQK17102">
        <property type="protein sequence ID" value="KQK17102"/>
        <property type="gene ID" value="BRADI_1g32490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836315"/>
    <dbReference type="Gramene" id="KQK17102">
        <property type="protein sequence ID" value="KQK17102"/>
        <property type="gene ID" value="BRADI_1g32490v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836315"/>
    <dbReference type="eggNOG" id="ENOG502QRB4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072626_4_1_1"/>
    <dbReference type="OMA" id="RSCDTQF"/>
    <dbReference type="OrthoDB" id="1476984at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047443">
        <property type="term" value="F:4-hydroxy-4-methyl-2-oxoglutarate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008428">
        <property type="term" value="F:ribonuclease inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.40">
        <property type="entry name" value="Ribonuclease E inhibitor RraA/RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010203">
        <property type="entry name" value="RraA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01935">
        <property type="entry name" value="NOT-MenG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006875">
        <property type="entry name" value="PRK09372.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254:SF30">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605493-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK17102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="168" mass="18036" checksum="B8EA32D9DB63A821" modified="2012-06-13" version="1">MAALPLATAEVCDANSHLITNGELRALQPIFQVYGRRQVFAGPIVTLKVFEDNVLVREFLEEKGQGRVLVVDGGGSLRCAILGGNPVQQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALNSHPMKANKKGMGEKHVPVTIAGTRICDGEWLYADTDGILVSRTELIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1H160</accession>
    <name>I1H160_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100823906</name>
        <name evidence="2" type="ORF">BRADI_1g49690v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557112.1">
        <property type="nucleotide sequence ID" value="XM_003557064.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H160"/>
    <dbReference type="FunCoup" id="I1H160">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK19667">
        <property type="protein sequence ID" value="KQK19667"/>
        <property type="gene ID" value="BRADI_1g49690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823906"/>
    <dbReference type="Gramene" id="KQK19667">
        <property type="protein sequence ID" value="KQK19667"/>
        <property type="gene ID" value="BRADI_1g49690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823906"/>
    <dbReference type="eggNOG" id="ENOG502S663">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113476_0_0_1"/>
    <dbReference type="OMA" id="REPVHEK"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF166">
        <property type="entry name" value="OS06G0128800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK19667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="21575" checksum="E34F1268D786BE8F" modified="2012-06-13" version="1">MSSAASKDFWHGGEGGGVALEVTVLSAESLRLPPSYSPLPRKLRPYVSVSSSSSSSSTGVASSSGREHSWDEGESSVVVVPVGAGFLEGREDVRVAVLSASGGPCPLHLLLGLGDTQLGWCRVPAADVLDGLRPPRALRRLSYSLRGSSSGQCCGVVHLAVRVLGDVRALSSSPLAPAAQCHRVAVGVPVSGPSAGAVVVGTPSPWGAWWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1H1S3</accession>
    <name>I1H1S3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Isopenicillin N synthase-like Fe(2+) 2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g51440v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H1S3"/>
    <dbReference type="EnsemblPlants" id="KQK19942">
        <property type="protein sequence ID" value="KQK19942"/>
        <property type="gene ID" value="BRADI_1g51440v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19942">
        <property type="protein sequence ID" value="KQK19942"/>
        <property type="gene ID" value="BRADI_1g51440v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010119_8_5_1"/>
    <dbReference type="InParanoid" id="I1H1S3"/>
    <dbReference type="OMA" id="HEEWIRI"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopenicillin N synthase-like Fe(2+) 2OG dioxygenase" evidence="3">
        <location>
            <begin position="35"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK19942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="156" mass="17788" checksum="A1E7C8D59726E2E5" modified="2012-06-13" version="1">MHGFFSDPQTTTTFIRLDHCPPWPNPELALGVGRHQDDVGRLDVKRRTDGQWVRVRPVEDSSLVGDIIQVWSNDRYESAEHRVSVNAEKERFSMPCFFNPGSGTIVEPLEELVSAENPARYHGYNSGRFFGTSRKNSNFRKLGVENIPRSRISGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I1H312</accession>
    <name>I1H312_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100829326</name>
        <name evidence="9" type="ORF">BRADI_1g55260v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the histone H1/H5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557467.1">
        <property type="nucleotide sequence ID" value="XM_003557419.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H312"/>
    <dbReference type="FunCoup" id="I1H312">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H312"/>
    <dbReference type="EnsemblPlants" id="KQK20548">
        <property type="protein sequence ID" value="KQK20548"/>
        <property type="gene ID" value="BRADI_1g55260v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829326"/>
    <dbReference type="Gramene" id="KQK20548">
        <property type="protein sequence ID" value="KQK20548"/>
        <property type="gene ID" value="BRADI_1g55260v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829326"/>
    <dbReference type="eggNOG" id="ENOG502S79K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052897_5_0_1"/>
    <dbReference type="OMA" id="HIQANHG"/>
    <dbReference type="OrthoDB" id="1110759at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF159">
        <property type="entry name" value="OS07G0184800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="8">
        <location>
            <begin position="48"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="177"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK20548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="248" mass="24992" checksum="F3D93F5AC74431F6" modified="2012-06-13" version="1">MATEVAAPEVPATEVESPAPAEVAEKKAAKPAKAAKAKKPAAAKKPSAHPSYAEMITAAIVALKERTGSSSVAIGKHIESKHGEQLPGNFRKLLAVQLKKLAATGKLTKVKNSFKLPAKAAAAAPAKAPAAKPKPAAKPKAAAKPAAKKPKVTKPAAKTKAATKVAKPKASPKPKAKTAAPKTKAPAAAATPKRGRQAKAARTSAKDAPGKKAPAAKKKAATPVKKAATPKKAAAAPVRKAAARKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1H3X4</accession>
    <name>I1H3X4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100827241</name>
        <name evidence="17" type="ORF">BRADI_1g58020v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230088.2">
        <property type="nucleotide sequence ID" value="XM_010231786.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H3X4"/>
    <dbReference type="STRING" id="15368.I1H3X4"/>
    <dbReference type="EnsemblPlants" id="KQK20993">
        <property type="protein sequence ID" value="KQK20993"/>
        <property type="gene ID" value="BRADI_1g58020v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827241"/>
    <dbReference type="Gramene" id="KQK20993">
        <property type="protein sequence ID" value="KQK20993"/>
        <property type="gene ID" value="BRADI_1g58020v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827241"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_106_1_1"/>
    <dbReference type="OMA" id="CIAGHHR"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.510.10:FF:000783">
        <property type="entry name" value="Proline-rich receptor-like protein kinase PERK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047117">
        <property type="entry name" value="PERK1-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982:SF10">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="159"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="95"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="467"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="488"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00047899"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK20993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="521" mass="55424" checksum="AC6A0A07E1E7DFE9" modified="2012-06-13" version="1">MASSDSSPPSTTPILATRASRRLSSSHKSSYGGLKLKPEQVALAGVGAALLLVAASCCFCCCRSKKKKSSSRPPPPYLDPAMRFYADTSGFKGDTTASYSPTPPHQWRVAPAPASPDTDSTAFSKSRLPPAMPPPPPVPAGAEKSAFSYADLAAATGGFSEANLLGQGGFGYVHRGELLLPSVTGKKKKKEKVAVAVKQLKAGSGQGEREFQAEVDMISRVHHRHLVSLLGYCIAGHHRLLVYAFVPNHTLEHHLHSASPPVDWPTRLRIAIGAAKGIAYLHEDCNPRIIHRDIKSANILLDDDFEAKVADFGLAKLSHGTETHVSTRVMGTFGYLAPEYALTGKLTEKSDVFSYGVMLLELLTGRRPSDRCHDGNGDDDDDNLVDWARPALARAQADGGYGALVDRRLRVEYDAAEAARVAACAAACVRHAGRRRPKMSLVVKALQGHVPLEALNVDAARGDTLSSVGSMPEFGNGGGYRRLGSGSGSSSTTASSTSSSSNYTAQMERIRNKAVAQPSPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>I1H5R2</accession>
    <name>I1H5R2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Peptidase M16 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100824526</name>
        <name evidence="14" type="ORF">BRADI_1g63160v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557963.1">
        <property type="nucleotide sequence ID" value="XM_003557915.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H5R2"/>
    <dbReference type="STRING" id="15368.I1H5R2"/>
    <dbReference type="EnsemblPlants" id="KQK21796">
        <property type="protein sequence ID" value="KQK21796"/>
        <property type="gene ID" value="BRADI_1g63160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824526"/>
    <dbReference type="Gramene" id="KQK21796">
        <property type="protein sequence ID" value="KQK21796"/>
        <property type="gene ID" value="BRADI_1g63160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824526"/>
    <dbReference type="eggNOG" id="KOG0959">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004639_1_0_1"/>
    <dbReference type="OMA" id="INQVMEH"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H5R2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000003">
        <property type="entry name" value="Insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000030">
        <property type="entry name" value="Insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000005">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000012">
        <property type="entry name" value="Protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="10">
        <location>
            <begin position="110"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="11">
        <location>
            <begin position="266"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="12">
        <location>
            <begin position="453"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="13">
        <location>
            <begin position="848"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="41"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="53"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK21796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1035" mass="118231" checksum="0FEF1B2BB1E34B55" modified="2012-06-13" version="1">MASAVSRDDELVVKSPNDNRSYRLLRLANGLCALLVHDPEIYADGCPVPNPSEDVDMENEDDDDDEEGEDDDEEYSDEEGEDDDDEEEEDEEDEDGSEPKRRKEKGGSEPVIKKAAAAMCVGMGSFADPPNAQGLAHFLEHMLFMGSSEFPDENEYDSYLSKHGGSSNAFTETEYTCYHFDVKREYLKGALDRFSQFFVSPLVKAEAMDREILAVDSEFNQVLQSDSCRLYQLQSHTCSHGHPLNRFTWGNKKSLVEAMGSGINLREEILQMYTSNYHGGMMKLVIIGGEPLDTLEAWTMELFSKVKAGPLLDISPKTNMPFWKSGKLHKLEAVRDVHSLYLSWTLPCLHKEYMKKPEDYLAHLLGHEGKGSLLYFLKAKGWASSLSAGVGSGGAQRSSYAYIFEMSIRLTDSGLKNLYEVITAVYQYLNLLKQSDPQEWIFKELQDIGYMEFRFAEEQPPDDYVVDLAENMLFYSEKHIVAGEYLYEGWEPELVKHVLSFFHPDNMRVDILSKLFDKQSQAIKCEPWFGSQYIEEDIPSSLIESWRNPGQIDANFHLPRKNEFIPGDFTLRNASIDKSLNDDNPRCIVDEPFIKLWYKMDSTFNVPRANTYFLISVKDGCSSLRNSVLTDLFANLLKDELNEVLYQAYVAKLETSLSVVGSNLELKLYGYNDKLAILLSNILAASQSFSPKIDRFEVIKEDLERAYKNTNMKPMSHSTYLRLQVLREIFWDVDEKLEVLATLTFSDLAAFVPELLSQLHIEGLCHGNLSGEEVIHISKIFRNTLSAQTLPEEARHGERVFCIPNGANFLRSVRVKNDPEENSVVEVYFPVEQDVGKEATRLRAITDLFSNIIEEPCFDQLRTKEQLGYTVDSSPRMTYRVLAYCFRVMSSKYSPVYLQSRIDNFINGLSSLLDALDEETFEHHKSGLIADKLEKDPSLSYQTGDYWSQIVDKRYMFDMSKLEAEELRTVRKEDVISWYDTYIRSSSPKRRRLAIHVYGCNSDIAEAAKLQEQSWTAIDDVKSLKVSSQFYSSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9H5</accession>
    <name>I1H9H5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100826726</name>
        <name evidence="6" type="ORF">BRADI_1g74430v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NET family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229167.1">
        <property type="nucleotide sequence ID" value="XM_010230865.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752244.1">
        <property type="nucleotide sequence ID" value="XM_014896758.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752245.1">
        <property type="nucleotide sequence ID" value="XM_014896759.2"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H9H5"/>
    <dbReference type="EnsemblPlants" id="KQK23533">
        <property type="protein sequence ID" value="KQK23533"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT78140">
        <property type="protein sequence ID" value="PNT78140"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT78141">
        <property type="protein sequence ID" value="PNT78141"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT78142">
        <property type="protein sequence ID" value="PNT78142"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT78143">
        <property type="protein sequence ID" value="PNT78143"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT78144">
        <property type="protein sequence ID" value="PNT78144"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826726"/>
    <dbReference type="Gramene" id="KQK23533">
        <property type="protein sequence ID" value="KQK23533"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78140">
        <property type="protein sequence ID" value="PNT78140"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78141">
        <property type="protein sequence ID" value="PNT78141"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78142">
        <property type="protein sequence ID" value="PNT78142"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78143">
        <property type="protein sequence ID" value="PNT78143"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78144">
        <property type="protein sequence ID" value="PNT78144"/>
        <property type="gene ID" value="BRADI_1g74430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826726"/>
    <dbReference type="eggNOG" id="ENOG502QQ6M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001229_0_0_1"/>
    <dbReference type="OMA" id="ERSNHMQ"/>
    <dbReference type="OrthoDB" id="10255522at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011684">
        <property type="entry name" value="NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051861">
        <property type="entry name" value="NET_actin-binding_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258:SF10">
        <property type="entry name" value="OS03G0161100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258">
        <property type="entry name" value="PROTEIN NETWORKED 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07765">
        <property type="entry name" value="KIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51774">
        <property type="entry name" value="NAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAB" evidence="5">
        <location>
            <begin position="14"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="382"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="641"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="760"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="879"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="998"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1068"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1327"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1541"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1685"/>
            <end position="1761"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1797"/>
            <end position="1824"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1947"/>
            <end position="1999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK23533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="2298" mass="262955" checksum="8B611CB9136158B2" modified="2012-06-13" version="1">MATLVRHDSNSTRYSWLWVSHISPKNSKWLQENLTDMDVMVKAMIKLINEDADSFARRAEMYYKKRPELMKHVEEFYRAYRALAERYDQATGALRQAHRTISEEFPNQMPSMSEESPSSQEVEPRTPEMQIPLRAPFDPDDLHKDALGVSPQLFTVKRSGTHPDEISSSRKGLKQFNDLFASCDSAHRVSFPDGKVRKGLSFESPDAKGKEDDIMKLQQEISKLLAESQSLKQQVSSESQRANNAESESQSLKDTISCLRSEKDAALLQYSVSTERFSALESELSKAHTELKKLSDYMVMEVEKLNCAESRNNTMQSELEILNQKIGLQEQELAQCRKEMEIFHSSLQDESAKRKQAEDDLCTIEKEYSKSQGEVRMMALEMRAANDRLSEFKEVKLNLEDTVCELKKDVAKLTEQKQFSELLIEELHGNIDSLGDSKRKIEREIQALTSTISQMNTAKDVALLQHQQCIEEVSDLESKLTKAQSERGKIELKVQILVQELEQKGEEADAIRAQLQDEHFNHMQKEAALLAMEDLHSQSQEEAKRLAQDLAQSNKKLGDLEHKNFKLHNMSQEHERMISELNSKNDASLLQQQKSLERVSCLEAQLLVAQLETEKIVKKAEKSSKRASYLESQLLVAHSETEKIVKKAQMLEQELEHKNKEVGNLQSTLQEEGQKCIRAETAFRRVEHLHLQLQEEAKTLAQNLETLSNKLGEVENERLDLQNISRELKSTISEMHSEKDAMLLQHQQSSDRVSCLEAQLFGIQSELEKNQQKVQLLEQQLKHKKKEVNGLQNNLEEESHKRMHAEAELTMVKNLLSKSQEEVRRLVMDLENLNNKFSDVQDSKLVVEELSCELRNAICVLNAEKEAALVQQRLSFEKVSDLNSEVSKIQLELEKTENKLQMLEQELVQKNGMVEFLQSSLQEEGKKRVQAETLLFSNKNLYSQSQQEVNRLALEVGTLNRKLNEVETLSSELKDTILLLNSEKDTTLLKHKQSLVRTSDLESELSEVQVELKNAEQKVQMLDKELKQKREEVDSLQISLGDEAQKRTEGEEALLMMTNEHSNSRVEVNRLALEISMLNRKLNEVENVSSELKKTISLLNSEKGTALLQQKESLVRVSNLEIKLSEVQAELESSEQKGQMLDKELKLKREEVDALQTSLKDEAQKHIEGEAALLMMTNLHSQSQEEVSMLVLEIERLNGKLNEMENSKMDLENMISKHAEDNRILGEQNLSSESIIRGLHDELDMLKEMQVNLKNEVGLQIGENEVLQQQLTHQIRDKEILGKQYCSLEIEMEALNRRAATLQQVLEEKVCGMEKLSDEFSILKKSFSNAIVEMEALKETVKELEDSESSLKYDVSLHSSEKDALALELHVLNKKYAEVSEQKSMFETSFSNVNSELAELRMKLEASEELSQSYLAEKDNILSQLESTTLSMKSLEDEHGDLGDKHSSLLAEKDLLYSQLRNLQDQLEIRNEQHEALLRLHQIQINDFEVAVSSLREKICHMDQMLDQEQQECTYASISALILHNSLADVKDKNFALFDECQKFMEATHSAEAMIARLEEEAKNEEEEKKALLNHNKSLRDWISQQIKILNICEDLGRPGVVHDEIMLQTLSLETFNHVKHKEESEHRNVFMEAELSVLGTILTQIVLDSRDLHWQKCELEKEAETGAAELLILQNKNHEHIKLNEQLGQRLQQGSEREEKLKIELNSVIAQLMQKDDKLCKADEKNQSLQETNQELCRVLRDLEASMEDAKGVKGELEKVITTLTEQVAERDNDFLLLCEAKVALQGEVDIHKQKEKSLMSTLEMVTAEAEQHEKEIVSLVSDMITCSVNVMIYEEHLLELMMECEALEIRMITERAMLMKEISSRNSYVDDLHKRIASMGGENAELKAEMSISQQFVASLSDQLARLEEGTLLLSELNKEGKLEFVQEDRRGSESQDKSSGVLELQNLIARVEALRVVILDAKDRRDKEFTESAAKLEAANVEIEDLKTRKVSCTERKEQNMDDDRQKYDADNSKGKQVQIMKDIELDQVSTCSRYGTGATVYPLGGDANAELDDEMLQLWETAEKDCKNQTAKSSSSEHDIQAVEEVKSEYPSFELARGRDLGIDRLEMSTASLEPQQLWSKNVLEKLTTDAQGLLIIQASIEEVKQKIEGTLKGKSPTSSEYSSVRSQLQEIEGSVMEQIDFNNSLTKKAENYPAFEVNAELEGYSSRRRISEQVQKGSEKVARLDLELQKIQYVLLKLEEEYEYKRVKVSDKRSRLLLRDYVYARKDKNDAAQKKKSRVPFCGCVRPKTRTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAQ5</accession>
    <name>I1HAQ5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100831615</name>
        <name evidence="8" type="ORF">BRADI_1g78027v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562215.1">
        <property type="nucleotide sequence ID" value="XM_003562167.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAQ5"/>
    <dbReference type="FunCoup" id="I1HAQ5">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HAQ5"/>
    <dbReference type="EnsemblPlants" id="KQK24080">
        <property type="protein sequence ID" value="KQK24080"/>
        <property type="gene ID" value="BRADI_1g78027v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831615"/>
    <dbReference type="Gramene" id="KQK24080">
        <property type="protein sequence ID" value="KQK24080"/>
        <property type="gene ID" value="BRADI_1g78027v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831615"/>
    <dbReference type="eggNOG" id="KOG1021">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039682_1_0_1"/>
    <dbReference type="OMA" id="VCQEVEH"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010417">
        <property type="term" value="P:glucuronoxylan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF229">
        <property type="entry name" value="GLUCURONOXYLAN GLUCURONOSYLTRANSFERASE IRX7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="88"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK24080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="441" mass="50141" checksum="828C7F69D5EFC501" modified="2012-06-13" version="1">MTRDPKLPKPPLPLPPAARRLAASPSLSDKLRRHSPWLLLLIWFALSFSLFLSAAPPAASPLLRRPPAFLHHKPRALAEETTSAPPPAVRIYVYDLPRRFNRDWLAADPRCARHLFAAEVALHEALLGHYSAVRPEDADLFFVPVYVSCNFSTPNGFPSLSHARGLLADAVDLVRREAPYWNRSAGADHVFVASHDFGACFHPMEDVAIADGIPDFLKRSILLQTFGVQGPHVCQEAEHVVIPPHVPPEVALEILELEKTRRDIFAFFRGKMEVHPKNISGRFYSKKVRTELLQRYGRNSKFYLKRKRYDNYRSEMARSLFCLCPLGWAPWSPRLVESVLLGCIPVIIADNIRLPFPSVLRWSDISLQVAEKDVASLEKVLDHVVATNLTVIQKNLWDPVKRKALVFNRRLEEGDATWQVLRELEVLLDRSQSSYVGSWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCD0</accession>
    <name>I1HCD0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100821711</name>
        <name evidence="6" type="ORF">BRADI_2g04180v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NET family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754844.1">
        <property type="nucleotide sequence ID" value="XM_014899358.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754845.1">
        <property type="nucleotide sequence ID" value="XM_014899359.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCD0"/>
    <dbReference type="STRING" id="15368.I1HCD0"/>
    <dbReference type="EnsemblPlants" id="KQK02866">
        <property type="protein sequence ID" value="KQK02866"/>
        <property type="gene ID" value="BRADI_2g04180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821711"/>
    <dbReference type="Gramene" id="KQK02866">
        <property type="protein sequence ID" value="KQK02866"/>
        <property type="gene ID" value="BRADI_2g04180v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821711"/>
    <dbReference type="eggNOG" id="ENOG502QQTB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032761_0_0_1"/>
    <dbReference type="OMA" id="TEKERCI"/>
    <dbReference type="OrthoDB" id="1877257at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HCD0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011684">
        <property type="entry name" value="NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051861">
        <property type="entry name" value="NET_actin-binding_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258">
        <property type="entry name" value="PROTEIN NETWORKED 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258:SF3">
        <property type="entry name" value="PROTEIN NETWORKED 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07765">
        <property type="entry name" value="KIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51774">
        <property type="entry name" value="NAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAB" evidence="5">
        <location>
            <begin position="13"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="182"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="460"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="562"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK02866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="633" mass="72068" checksum="AE2C19115D8450AE" modified="2012-06-13" version="1">MKRMQRMPTKKSHSWWWDSHISPKNNKWLADNLEEMDKQVKEMLKLIEDEGDSFAKKAEMYYQRRPLLVTHVENFYRMYRSLAERYDNVTVELRKNIPSSLQSQGSGISESDSEAQSTSPSPEKKKTPKQKSKIRAAGFDVFLGSGGSSDISKKGSDGSSSSSSESDSEVDEVGEENGNGVSYALNERIIELEDELQEAREKLEALEEKNVRCQCEKLEEKLLASQSEINSLQKDLEEKVSCLENLKEVTSGKEDLEAAALENKNTIKVLEGEMASAAEHYEHQLSYRDLEIEKCKQELEQVSQKYSHDKSTLETEIKKLQEAVKNLEGDLVKMSQEKLQLEAQVMELEQTSHSLNDSSAEITKLQKLIKDLQARLENDSNEKRVLEERAIKFEQIHRELEHSRAETVELQNTIKNLKGDLEGAFQEKSKLEGCVKDLEQAIAHNLEKFSLEKSPLSAEILQLSEANASLEGKLTSTEEQLKQLHAEKGEASLESEKLISELSQDIDNLKTKLELLSSEKAAVDNKVSSLLTDLTTRDEKINEMGSHLHQLHLEHVKLIAEADTARQSVSDLRAQVHELEEEVEKQKLMIFDSAEGKREAIRQLCFSLDHYRHGYQQLRQVLQGHKRPMVMAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1HCV6</accession>
    <name>I1HCV6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4005 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100844448</name>
        <name evidence="6" type="ORF">BRADI_2g05680v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Binds to multiple calmodulin (CaM) in the presence of Ca(2+) and CaM-like proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564676.1">
        <property type="nucleotide sequence ID" value="XM_003564628.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HCV6"/>
    <dbReference type="STRING" id="15368.I1HCV6"/>
    <dbReference type="EnsemblPlants" id="KQK03112">
        <property type="protein sequence ID" value="KQK03112"/>
        <property type="gene ID" value="BRADI_2g05680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844448"/>
    <dbReference type="Gramene" id="KQK03112">
        <property type="protein sequence ID" value="KQK03112"/>
        <property type="gene ID" value="BRADI_2g05680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844448"/>
    <dbReference type="eggNOG" id="ENOG502QVYZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042730_3_0_1"/>
    <dbReference type="OMA" id="HSVAPYY"/>
    <dbReference type="OrthoDB" id="1704267at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025064">
        <property type="entry name" value="DUF4005"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF290">
        <property type="entry name" value="OS01G0190500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13178">
        <property type="entry name" value="DUF4005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4005" evidence="5">
        <location>
            <begin position="344"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="377"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK03112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="493" mass="52909" checksum="B36B03DF91D619A5" modified="2012-06-13" version="1">MGKAARWLRNLLGGGGKKEQGREREQRRPATTNNAAAPSGDRKRWSFCKSSSRDTSEPEVTAAAQVGANNGVAAIARAAEAAWLRSLYKETEREQSKHAIAVAAATAVAADAAVAAAQAAVEVVRLTSQGPLVAATSPRAFAGAGGDGRAAAAVRIQTAFRGYLAKKALRALKALVKLQALVRGYLVRKQAAATLQSMQALVRAQAAIRAAARNRAADLRLHLHPLTVRPPRYSLQEDTRSEHGSGVAPYYYSRRLSASVESSSYGYDRSPKIVEMDTARPKSRSSSLPVAEPGGGDDYGYYSVSSPLMPCGHGLPCAPPRIAAPSRGFFLPEYYEREKPRPATAQSTPRYASSLYYTPVTPAKSVCGVGGYSNNSPSTLLSGPRSYMSSTQSSDAKTRSQSAPKQRPEEGGAVPPRKRVPLREVVLEARASLSGATQRYYASSCNNSNNRPPREEAGEKFSFKKAVVSRFDRSSEPAVAADRDRDMFLQKGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1HDU1</accession>
    <name>I1HDU1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Negatively light-regulated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100844259</name>
        <name evidence="4" type="ORF">BRADI_2g08560v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566309.1">
        <property type="nucleotide sequence ID" value="XM_003566261.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HDU1"/>
    <dbReference type="STRING" id="15368.I1HDU1"/>
    <dbReference type="EnsemblPlants" id="KQK03558">
        <property type="protein sequence ID" value="KQK03558"/>
        <property type="gene ID" value="BRADI_2g08560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844259"/>
    <dbReference type="Gramene" id="KQK03558">
        <property type="protein sequence ID" value="KQK03558"/>
        <property type="gene ID" value="BRADI_2g08560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844259"/>
    <dbReference type="eggNOG" id="ENOG502S1VS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094393_3_0_1"/>
    <dbReference type="OMA" id="NYTQEQD"/>
    <dbReference type="OrthoDB" id="1902088at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF45">
        <property type="entry name" value="OS01G0249300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="68"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK03558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13775" checksum="69F3C553E12F527C" modified="2012-06-13" version="1">MSEMKDTNATGHDLVGNSEPMDQSADNSMPSVQQQEQAIKKKFGGLIPKKTPLISKDHERAYFDSADWALGKSGQGVSKPKGPLEALRPKLQPTRQQQQRSRRTIYTSSENEDGEGASAEDMNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HI64</accession>
    <name>I1HI64_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Association with the SNF1 complex (ASC) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100837253</name>
        <name evidence="4" type="ORF">BRADI_2g21420v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568206.1">
        <property type="nucleotide sequence ID" value="XM_003568158.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HI64"/>
    <dbReference type="STRING" id="15368.I1HI64"/>
    <dbReference type="EnsemblPlants" id="KQK05651">
        <property type="protein sequence ID" value="KQK05651"/>
        <property type="gene ID" value="BRADI_2g21420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837253"/>
    <dbReference type="Gramene" id="KQK05651">
        <property type="protein sequence ID" value="KQK05651"/>
        <property type="gene ID" value="BRADI_2g21420v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837253"/>
    <dbReference type="eggNOG" id="KOG1616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070949_0_0_1"/>
    <dbReference type="OMA" id="QRTINAP"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043554">
        <property type="entry name" value="KINB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316">
        <property type="entry name" value="SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316:SF9">
        <property type="entry name" value="SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="3">
        <location>
            <begin position="185"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK05651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="277" mass="30375" checksum="DE02BD703FA359DC" modified="2012-06-13" version="1">MGNASAKEVENGHSAESLELGGTAGGGLTEAAAPPDAVMRELPPPVPFVFAPQVPVTPLQRLTEISTVFNHSWTNGLDEPTNDNPQEKGIPTLITWRQGGNEVLVEGSWDDWTSRKALQRSGKDHAILLVLPSGVYHYRIIVEGQPRYVPELPHVTDERGQVANLLDVHDYVPESLDSVAEFDAPPSPEHSYDLQFPADEEFAKEPPALPPQLLMSVLGGADNADQHAPKPKPQHVVLDHLFIEKGWGSQSLLALGVTHRFQSKYVNFVLYKPLLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HLX2</accession>
    <name>I1HLX2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Disease resistance N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_2g36170v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK07545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HLX2"/>
    <dbReference type="STRING" id="15368.I1HLX2"/>
    <dbReference type="EnsemblPlants" id="KQK07545">
        <property type="protein sequence ID" value="KQK07545"/>
        <property type="gene ID" value="BRADI_2g36170v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07545">
        <property type="protein sequence ID" value="KQK07545"/>
        <property type="gene ID" value="BRADI_2g36170v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000837_20_3_1"/>
    <dbReference type="InParanoid" id="I1HLX2"/>
    <dbReference type="OMA" id="FLFCENT"/>
    <dbReference type="OrthoDB" id="666944at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rx_N"/>
    </dbReference>
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        <property type="entry name" value="Rx_N"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094636" description="Disease resistance N-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="26"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="13"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK07545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="108" mass="12100" checksum="C465E5741580B64D" modified="2012-06-13" version="1">MAHVGGMLAAAILKVLVQQIGSAIGSQIALHMNLRKDLAKMKMTLESVEAVLEDADRRSITDKPTRLWLKRLKDAMYAISDMIDEFEAIAQPSKQKVRVREKKILKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HM33</accession>
    <name>I1HM33_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100835844</name>
        <name evidence="5" type="ORF">BRADI_2g36647v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568966.1">
        <property type="nucleotide sequence ID" value="XM_003568918.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HM33"/>
    <dbReference type="STRING" id="15368.I1HM33"/>
    <dbReference type="EnsemblPlants" id="KQK07624">
        <property type="protein sequence ID" value="KQK07624"/>
        <property type="gene ID" value="BRADI_2g36647v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK07625">
        <property type="protein sequence ID" value="KQK07625"/>
        <property type="gene ID" value="BRADI_2g36647v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835844"/>
    <dbReference type="Gramene" id="KQK07624">
        <property type="protein sequence ID" value="KQK07624"/>
        <property type="gene ID" value="BRADI_2g36647v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07625">
        <property type="protein sequence ID" value="KQK07625"/>
        <property type="gene ID" value="BRADI_2g36647v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835844"/>
    <dbReference type="eggNOG" id="ENOG502QWJB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012143_0_1_1"/>
    <dbReference type="OMA" id="CAMDYST"/>
    <dbReference type="OrthoDB" id="774873at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23128">
        <property type="entry name" value="RING-HC_MIP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046934">
        <property type="entry name" value="PIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046527">
        <property type="entry name" value="PIR2-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46405">
        <property type="entry name" value="OS05G0141500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46405:SF2">
        <property type="entry name" value="OS05G0141500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20235">
        <property type="entry name" value="PIR2-like_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="798"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="272"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="331"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="434"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="535"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="718"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="435"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK07625.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK07624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="852" mass="93364" checksum="71285EAF678F82D8" modified="2012-06-13" version="1">MSSVATQEAASGSASASQEKAASRNKRKYRAEPPSGELGPFGLEYPLTTDCVGFEFMSPEKAAMAAYATASEGANLDFTPSACDGCKAVHATAEELLECQRHVNWSDPNEAQLEEILLKCLDTTFDNAVSVIISMGYSEAGARAAVVRAAAQYTWRESLAGFSEAAVEVLKSEGDMLPMDGSSLEDMRKIEKAVLGSLVAVVNEAQPFYTTGDAMFCLLMSDMNVAHACAMDYSSASLPPVGAQVVAQPVVGNHEPGPSSDVSVKITNPQTGVTFRGKLTPVPPASLNMPSGKPSISGKMHPLSPNLKHKEHPVAMPDHSEDQPFVAAATQSVKDDKPFSSKRGSSKRDSLHRQKLMSFDKNSRALGSKGSLRSGKHSSSGIAALERKCRPFADATTSSLKGPVKVGKGFATGMTGSEYSGDLSFTATGTIAPLPSFDTKTTSSTDSASTASTELSLSLPLPLPSSSDVSAPSLNQDSKTEAVDPSSKINFTYDENQKVWIPQEKKDEMVLVLVQRQKELQAHMRDWTEWAMEKVMLVTRRLAKEKEELQSLRKEKEEADRLQEERHCLEESTRKKLLEMESAISRANTQLDKADAAGRRRITENTQLRMQMEAAKRHAAESAANFVELSKKDESSLKRSQHWESERTLLQEELAAGKSKLSRVQQQLQSSKEKKEQLKVRWRQEEAAKAEAIARVTSERKERDQIETSLRSEENFLHLKAENDMQRFKSEIRALEHQITQLELSMDALDEAGVPSDKIRSLSLSEGRKIGNTQILAKVAAAASQDLDLDDIQRDRECVMCLSEEMSVVFLPCAHQVVCAKCNDLHEKQGMKECPSCRTHIQRRVCARPAGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HNX9</accession>
    <name>I1HNX9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF632 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100827275</name>
        <name evidence="4" type="ORF">BRADI_2g42060v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231954.1">
        <property type="nucleotide sequence ID" value="XM_010233652.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HNX9"/>
    <dbReference type="STRING" id="15368.I1HNX9"/>
    <dbReference type="EnsemblPlants" id="KQK08470">
        <property type="protein sequence ID" value="KQK08470"/>
        <property type="gene ID" value="BRADI_2g42060v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827275"/>
    <dbReference type="Gramene" id="KQK08470">
        <property type="protein sequence ID" value="KQK08470"/>
        <property type="gene ID" value="BRADI_2g42060v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010985_5_1_1"/>
    <dbReference type="OrthoDB" id="1919226at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HNX9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006868">
        <property type="entry name" value="DUF630"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006867">
        <property type="entry name" value="DUF632"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21450">
        <property type="entry name" value="PROTEIN ALTERED PHOSPHATE STARVATION RESPONSE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21450:SF21">
        <property type="entry name" value="REDUCTASE SUBUNIT C, PUTATIVE (DUF630 AND DUF632)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04783">
        <property type="entry name" value="DUF630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04782">
        <property type="entry name" value="DUF632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF630" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="DUF632" evidence="2">
        <location>
            <begin position="264"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK08470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="698" mass="77517" checksum="D6B8B811A71F47F1" modified="2012-06-13" version="1">MGCRHSKVKDEEAVRRCRDRKKLMKQLVSRRVDLAAAHITYLQALRNTGATLRQFAEVESALSQQSPESLSVPVSPTLTPPPPPPPGPPQPAAYSMTSSMPPHSVTSSMPPSPLAVPFLPFSPIRIRKREKRDGELHGDDSTDDDDDTDSCSTPLPPPPPPGMEWEHIDPITMRPLNFPSPFTGGNDKDVASQVTMDDDWVETNTESDGCEGESISGNFDGIVRRAELHSAKSRALGDENSSVVSWVTKDLDSSAVAWRSEKSLVEIAKEIDEYFLKAAASGSDVVILLDSASGRLDLSELEAKKGAGKNSKSAKVFSTLSWSWSFKSVQVNRESSDGACGYGYHGKTLDKLYDEEQRLYKLVKDEEFARLQYRKYNSLLQRLESGEHDKLHAEKVRDNIELLQTQIISLEEAASSTCLTISKIRDEELYPQVIELCAGLVHMWRNMHECHQVQNHIAQQASLLGNRPGSEPTTDSHCYATSQLEMEVSAWHSSFCNLITLQREYVTILNQWIRLTDCLPDNDGFMRSSSGICSLCGELQHALEKIPEKEPAEAIKTFLSVIHSIVTQQAEERQLKKKSDNIESKFHTQLEKHSENAMQTSGQPTNARNHSVSKDDPKLEAFRKRVEEEKARYRNSLRTSRAMTLNNLQTSLPNVFHALMGFSGVCVQAFEGISRCSEAVASHSGAASPAVSSCDHPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1HP70</accession>
    <name>I1HP70_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100831632</name>
        <name evidence="16" type="ORF">BRADI_2g42890v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231995.1">
        <property type="nucleotide sequence ID" value="XM_010233693.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HP70"/>
    <dbReference type="STRING" id="15368.I1HP70"/>
    <dbReference type="EnsemblPlants" id="KQK08619">
        <property type="protein sequence ID" value="KQK08619"/>
        <property type="gene ID" value="BRADI_2g42890v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831632"/>
    <dbReference type="Gramene" id="KQK08619">
        <property type="protein sequence ID" value="KQK08619"/>
        <property type="gene ID" value="BRADI_2g42890v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831632"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_18_3_1"/>
    <dbReference type="OMA" id="DYRENTI"/>
    <dbReference type="OrthoDB" id="612781at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000649">
        <property type="entry name" value="Leucine Rich Repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF108">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="927"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="42"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="115"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK08619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="985" mass="108573" checksum="F21F3468E0A3BD29" modified="2012-06-13" version="1">MAETRRSSSHASAANFLFIIVTATTIFSVTYASEILNGSCIPTERAALLSFKAGVTSDPASRLDSWSGHGCCHWSGVSCSVRTGHVVELDLHNDHFFAELSGADAPHSMSGQISSSLPALRHLKHLDLSGNYLGNGMPIPEFVGSLKRLTYLDLSNMNFIGTVPPQLGNLSKLVHLDISSVYFPTHSMDISWLARLQSLEHLNMGTVNLSAAVDWVHSVKALPNLIVLKLEFCSLNSKSAPSLLQHNLTVLEELDLSRNTLNSPAAQNWFWGVTSLKWLHLFNCGLSGTFPDELGNLTSLEALDLGGNNMKGMMPATLKNLCSLRYLYIDNNNIGGDITDLIERLLCSWKSLQELNLMEANISGTTLEAVANLTSLSWFDVTNNHLSGSVPVEIGTLANLSVFILTNNNLSGVISQEHFAGLTNLKEIDLSYNNLKIITDFDWIPPFKLDIARFGSCLLGPRFPEWLRGQNGISDLNISRTGLISTIPDWFWTTFSNAVHLDISSNQLSGELPVTLESLSVITLFAQANRLTGSVPQLSNEIQILDISRNFLNGSLPSNNRATRLSIAVLFSNRITETIETAICQWTDLCVLDLSNNLFVGDFPDCGREELKQWKPSSDNSSRDSIDSSGSKIEILLLSNNNLSGGFPLFLRQCRSLIFLDLTQNKFTGKLPAWISEDMPYLLMLRLRSNNFSGRIPNELLGLIALRILDLSNNSFSGSIPRSLGNLTALTATVEGFHADNPFNEYYLSGPLTMSSNGQFNDSLSVVIKGQVLDYRENTIYLMSIDLSCNSLAGEIPEELSSLAGLINLNLSSNLLSGNIPYKIGNLRSLESLDLSKNKLDGVIPWGLSDLTYLSYLNLSYNNLSGRIPSGHQLDILKADDPASMYFGNPGLCGHPIPRQCPGPPGDPSTPGDSARWHDDGLPQMDFLLGFIVGFVAGVWMLFCGLLFKKRWRYAYFGQLDKLYDKVYVTAVITWRKWFRNTVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPQ1</accession>
    <name>I1HPQ1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100843875</name>
        <name evidence="18" type="ORF">BRADI_2g44400v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232060.1">
        <property type="nucleotide sequence ID" value="XM_010233758.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPQ1"/>
    <dbReference type="STRING" id="15368.I1HPQ1"/>
    <dbReference type="EnsemblPlants" id="KQK08864">
        <property type="protein sequence ID" value="KQK08864"/>
        <property type="gene ID" value="BRADI_2g44400v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK08865">
        <property type="protein sequence ID" value="KQK08865"/>
        <property type="gene ID" value="BRADI_2g44400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843875"/>
    <dbReference type="Gramene" id="KQK08864">
        <property type="protein sequence ID" value="KQK08864"/>
        <property type="gene ID" value="BRADI_2g44400v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08865">
        <property type="protein sequence ID" value="KQK08865"/>
        <property type="gene ID" value="BRADI_2g44400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843875"/>
    <dbReference type="HOGENOM" id="CLU_000288_4_1_1"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HPQ1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000083">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000035">
        <property type="entry name" value="Putative receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052232">
        <property type="entry name" value="RLK_Ser/Thr-Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984">
        <property type="entry name" value="OS01G0323000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984:SF4">
        <property type="entry name" value="OS01G0631700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="188"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="83"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="459"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="496"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK08865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="511" mass="57090" checksum="BD33F91853045276" modified="2012-06-13" version="1">MGSTDLNSEMTRTVLGLTLWIWIAIGVVALLVAILLMICIWVASRRRTKRTIDNMTQTQIPIYSKEIPVDRVGGGRSLAQTMHEREQPSFPPQDKHANREPGKTPGHMALSKSSDHDNMSQGSSVCNVDRGGSVHSGEDGSSGQHGRKPYSPAAFVSASPLVGLPEFSHLGWGHWFTLRDLEFATNRFSKENVLGEGGYGVVYRGRLVNGTDVAIKKIFNNMGQAEKEFRVEVEAIGHVRHKNLVRLLGYCVEGVNRMLVYEFVNNGNLEQWLHGAMRQHGVFSWENRMKVVIGTAKALAYLHEAIEPKVVHRDIKSSNILIDDEFNGKVSDFGLAKMLGSDKSHITTRVMGTFGYVAPEYANTGMLNEKSDVYSFGVLLLETVTGRDPVDYSRSANEVNLVEWLKMMIANRRAEEVVDPILEVRPTIRALKRALLIALRCVDPDSEKRPKMGQVARMLESEEVPYREDRRNRRSRTGSMDIESITEGSNSAEFANKVERTGSSRSDRSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HPV1</accession>
    <name>I1HPV1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin efflux carrier component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100826548</name>
        <name evidence="10" type="ORF">BRADI_2g44987v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May act as a component of the auxin efflux carrier.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the auxin efflux carrier (TC 2.A.69.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232076.1">
        <property type="nucleotide sequence ID" value="XM_010233774.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HPV1"/>
    <dbReference type="FunCoup" id="I1HPV1">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HPV1"/>
    <dbReference type="EnsemblPlants" id="KQK08941">
        <property type="protein sequence ID" value="KQK08941"/>
        <property type="gene ID" value="BRADI_2g44987v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826548"/>
    <dbReference type="Gramene" id="KQK08941">
        <property type="protein sequence ID" value="KQK08941"/>
        <property type="gene ID" value="BRADI_2g44987v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826548"/>
    <dbReference type="eggNOG" id="ENOG502QRM7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019285_1_1_1"/>
    <dbReference type="OMA" id="MISWKDF"/>
    <dbReference type="OrthoDB" id="1868374at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009921">
        <property type="term" value="C:auxin efflux carrier complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010329">
        <property type="term" value="F:auxin efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010315">
        <property type="term" value="P:auxin export across the plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009926">
        <property type="term" value="P:auxin polar transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014024">
        <property type="entry name" value="Auxin_eff_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051107">
        <property type="entry name" value="Auxin_Efflux_Carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00946">
        <property type="entry name" value="2a69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31752">
        <property type="entry name" value="AUXIN EFFLUX CARRIER COMPONENT 1B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31752:SF72">
        <property type="entry name" value="AUXIN EFFLUX CARRIER COMPONENT 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="505"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="562"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="234"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="358"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK08941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="590" mass="62913" checksum="DBF785974800EFDB" modified="2012-06-13" version="1">MISSHDLYTVLAAVVPLYVAMILAYGSVRWWGIFTPDQCSGINRFVAIFAVPLLSFHFISASDPYQMNLRFLAADTLQKLLVLAALAACSRLIPSLGLDWSVTLFSLSTLPNTLVMGIPLLVAMYGPDAGSLMVQIVVLQCIIWYTLLLFLFEFRAARALIADQFPDTAASIASLHVDPDVVSLDGGRAETEAEVAPDGRLRVTVRRSSVSRQSLSLLGVTPRPSNLTGAEIYSVNSSQNPTPRGSNFNHADFFATVGAPPPPTTPAGARGSSFGTSSEHFSLHTSSQGPTPRGSNFDEHSAGAGGRKPAAAGAHSSHDAKELHMFVWSSSTSPVSEVSRLPADRARLDAKEIRMVVPADPPQNGSCKEAAVEDDGEAAEAGRERLTKLSSSSTAELRPNVYADTAGAEDAKRPRQQHQMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLVWSLAAFRWHISMPSIVGKSISILSDAGLGMAMFSLGLFMALQPSIIACGNTVAVVSMVVRFIVGPAVIAAASAAVGLRGTLFKISIVQAALPQGIVPFVFAKEYNVHPTILSTAVIFGMLVALPITLAYYAILGLVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSA5</accession>
    <name>I1HSA5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100823241</name>
        <name evidence="6" type="ORF">BRADI_2g51860v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564359.1">
        <property type="nucleotide sequence ID" value="XM_003564311.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSA5"/>
    <dbReference type="EnsemblPlants" id="PNT72990">
        <property type="protein sequence ID" value="PNT72990"/>
        <property type="gene ID" value="BRADI_2g51860v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72991">
        <property type="protein sequence ID" value="PNT72991"/>
        <property type="gene ID" value="BRADI_2g51860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823241"/>
    <dbReference type="Gramene" id="PNT72990">
        <property type="protein sequence ID" value="PNT72990"/>
        <property type="gene ID" value="BRADI_2g51860v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72991">
        <property type="protein sequence ID" value="PNT72991"/>
        <property type="gene ID" value="BRADI_2g51860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823241"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_49_13_1"/>
    <dbReference type="OMA" id="CAAKRWE"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000294">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At1g09900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932">
        <property type="entry name" value="ATPASE EXPRESSION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932:SF45">
        <property type="entry name" value="OS02G0565400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="69"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="136"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="171"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="206"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="241"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="276"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="311"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="346"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="381"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="416"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="448"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="483"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="518"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="553"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="588"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="623"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT72990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="665" mass="73853" checksum="E9B848DBC6AD9C10" modified="2012-06-13" version="1">MATLLPSFPHSLSKPRHLRHSHIAAAATRPEAAAPSPASSRLRRLIARDDLAEAARLVERSTSRGEAPDVYLCTKLIRNLCRRGRTSDAARVLRTAEGSGAPVDVFAYNTLVAGYCRYGRLDAARRLIASMPVPPDAYTYTPLIRGLCDRGRVGDALSLLDDMLRRECQPSVVTYTVLLEAVCKSSGFGQAMKVLDEMRAKGCTPNIVTYNVIINGMCREDRVDDARQILNRLSSYGFQPDTVSYTTVLKGLCAAKRWEDVEVLFCEMVENNCVPNEVTFDMLVRFFCRGGMVERAIEVLDRMSEHGCTANTTLCNIVINSICKQGRVDDAFEFLNNMGSYGCSPDTISYTTVLKGLCRAGRWEDAKELLNEMVRKNCPPNEVTFNTFICILCQKGLIDQAILLIEQMPEYGCSVGIVTYNALVNGFCVQGRVDSALELFNSLPCEPNTITYTTLLTGLCHAERLDAAAELLAEMMQNDCPLNVVTFNVLVSFFCQKGFVEEAIELVQQMMEHGCTPNLITFNTLLDGITEDCNSEEALELLHGLVSKGISLDTITYSSIVDVLSREDRIEEAVQMFHAVQDMGMRPKAVMYNKILSALCKRCETDRAIDFFAHMVSNSCMPNESTYVILIEGLAREGLLKEARYVLSELCSRGVLSKSLIEDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSR1</accession>
    <name>I1HSR1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100830300</name>
        <name evidence="5" type="ORF">BRADI_2g53140v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564466.3">
        <property type="nucleotide sequence ID" value="XM_003564418.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSR1"/>
    <dbReference type="EnsemblPlants" id="KQK10279">
        <property type="protein sequence ID" value="KQK10279"/>
        <property type="gene ID" value="BRADI_2g53140v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73091">
        <property type="protein sequence ID" value="PNT73091"/>
        <property type="gene ID" value="BRADI_2g53140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830300"/>
    <dbReference type="Gramene" id="KQK10279">
        <property type="protein sequence ID" value="KQK10279"/>
        <property type="gene ID" value="BRADI_2g53140v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73091">
        <property type="protein sequence ID" value="PNT73091"/>
        <property type="gene ID" value="BRADI_2g53140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830300"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_37_2_1"/>
    <dbReference type="OMA" id="ASNSQWD"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000913">
        <property type="entry name" value="Os01g0814300 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000404">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF461">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="193"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="263"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="329"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="508"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK10279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="600" mass="66402" checksum="30AFFFB50603F571" modified="2012-06-13" version="1">MQHQLLRPHDAASLPRHLLEARVVSLVRRCPGLRALRGAHAHFLRLRLPRLTYAFALSKLLASCAAASATTVASSYARALFDQIPEPTAFCYNSLIRAVSGSGSSSNSGTTDTFLLYRRMLHAGSPAPNSFTLAFVLKACTALGEGQQLHAQAFGHGLEPSPYVQTGLLNLYARCEEVALARNVFDGMVEDKNLVAWSSMIGGYSRMGMVNEALGLFRDMQAVGVNPDEVTMVSVISACAKAGALDLGKWVHAFIDRKGITVDLELSTALIDMYAKCGLIERAKSVFDSMVERDTKAWSAMIVGLAMHGLAEDALGLFSRMLQLKVRPNNVTFVGVLSACAHSGLVDDGRRYWCTMQELGIEASMENYGCMVDLLCRSGLLDEAFSFVTGMPISPNSVIWRNLLVASKSSNRIDIVELASRRLFELEPQNPENYVLLSNLYALNSQWDRVRYMRKKMKDNNVTVVAGCSSIEINGYLHKFVVSDGSHPEIKKIRLVLREIADRVLCAGHKPWTAAVLHDVVEEEKEVALCEHSERLAIAYGLLKTKAPHVIRVVKNLRFCPDCHEVTKIISKSYGREIIVRDRVRFHRFIGGSCSCNDFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HT28</accession>
    <name>I1HT28_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100845284</name>
        <name evidence="12" type="ORF">BRADI_2g54090v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>jasmonate + 2-oxoglutarate + O2 = (1R,2R)-12-hydroxyjasmonate + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:67144"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58431"/>
            <dbReference type="ChEBI" id="CHEBI:132022"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:67145"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567249.1">
        <property type="nucleotide sequence ID" value="XM_003567201.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232514.1">
        <property type="nucleotide sequence ID" value="XM_010234212.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753828.1">
        <property type="nucleotide sequence ID" value="XM_014898342.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HT28"/>
    <dbReference type="EnsemblPlants" id="KQK10429">
        <property type="protein sequence ID" value="KQK10429"/>
        <property type="gene ID" value="BRADI_2g54090v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK10430">
        <property type="protein sequence ID" value="KQK10430"/>
        <property type="gene ID" value="BRADI_2g54090v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73133">
        <property type="protein sequence ID" value="PNT73133"/>
        <property type="gene ID" value="BRADI_2g54090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845284"/>
    <dbReference type="Gramene" id="KQK10429">
        <property type="protein sequence ID" value="KQK10429"/>
        <property type="gene ID" value="BRADI_2g54090v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10430">
        <property type="protein sequence ID" value="KQK10430"/>
        <property type="gene ID" value="BRADI_2g54090v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73133">
        <property type="protein sequence ID" value="PNT73133"/>
        <property type="gene ID" value="BRADI_2g54090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845284"/>
    <dbReference type="eggNOG" id="KOG0143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010119_16_0_1"/>
    <dbReference type="OMA" id="PAFWHGD"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120091">
        <property type="term" value="F:jasmonic acid hydrolase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900366">
        <property type="term" value="P:negative regulation of defense response to insect"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900150">
        <property type="term" value="P:regulation of defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000008">
        <property type="entry name" value="Jasmonate-regulated gene 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="11">
        <location>
            <begin position="213"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK10429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="378" mass="41759" checksum="BC65F07908A0E5EB" modified="2012-06-13" version="1">MADCMQEWPEPVVRVQAVAESGLAAIPCRYVKPPRDRPSATPTPSDNDDDDIAIPVIDLGALLAADEEGRVTMSKAVAAACREWGFFQVVNHGVRPELMRAAREAWRGFFRLPIPAKQQYANLPRTYEGYGSRVGVQKGGPLDWGDYYFLHLAPDAAKSPDKYWPTNPGICKEVSEEYGREVIGLCERLMKVMSASLGLDETRFQEAFGGSDCGACLRANYYPRCPQPDLTLGLSAHSDPGVLTVLLADEHVRGLQVRRRGGVDGDWVTVQPVPDAFIVNVGDQIQIMSNSVYKSVEHRVIVNAMEERISLALFYNPRGDLPIAPASELVTRGRPSLYPRPMTFDEYRVYIRKNGPKGKAQLEGLKAAQAVPAENELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HU38</accession>
    <name>I1HU38_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic</fullName>
            <ecNumber evidence="3">2.7.7.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100829889</name>
        <name evidence="8" type="ORF">BRADI_2g57260v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.60" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564801.1">
        <property type="nucleotide sequence ID" value="XM_003564753.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HU38"/>
    <dbReference type="STRING" id="15368.I1HU38"/>
    <dbReference type="EnsemblPlants" id="KQK10954">
        <property type="protein sequence ID" value="KQK10954"/>
        <property type="gene ID" value="BRADI_2g57260v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829889"/>
    <dbReference type="Gramene" id="KQK10954">
        <property type="protein sequence ID" value="KQK10954"/>
        <property type="gene ID" value="BRADI_2g57260v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829889"/>
    <dbReference type="eggNOG" id="ENOG502QUUE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061281_0_1_1"/>
    <dbReference type="OMA" id="TPMLIHA"/>
    <dbReference type="OrthoDB" id="414267at2759"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00093"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050518">
        <property type="term" value="F:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02516">
        <property type="entry name" value="CDP-ME_synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000003">
        <property type="entry name" value="2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00108">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001228">
        <property type="entry name" value="IspD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034683">
        <property type="entry name" value="IspD/TarI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050088">
        <property type="entry name" value="IspD/TarI_cytidylyltransf_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018294">
        <property type="entry name" value="ISPD_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00453">
        <property type="entry name" value="ispD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32125">
        <property type="entry name" value="2-C-METHYL-D-ERYTHRITOL 4-PHOSPHATE CYTIDYLYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32125:SF4">
        <property type="entry name" value="2-C-METHYL-D-ERYTHRITOL 4-PHOSPHATE CYTIDYLYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01128">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01295">
        <property type="entry name" value="ISPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK10954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="294" mass="32528" checksum="2618B501AA74A99D" modified="2012-06-13" version="1">MELRLCLRLHARLPSAPKRSRLPSPAMVLLPASGRLRIGWSCGDAPRRIPNSAIFAVRADGAQGDAVKERSVSVILLSGGQGKRMGANMPKQYIPLLGTPIALHSLRTFCRMKEVKEVIVVCDPSYRNVFEDSIENLQIPIKFACPGKERQDSVFNGLQEIDRDSELVCVHDSARPLVSSKDVKKVLEDAAVHGAAVLGVPVKATIKEASSDSFVVKTLDRKTLWEMQTPQVMEPNLLKDGFDLVKRDGLEVTDDVSIVEYLKHPVYITEGSYTNIKVTTPDDLLLAERLMNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUS7</accession>
    <name>I1HUS7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100832428</name>
        <name evidence="8" type="ORF">BRADI_2g59410v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564980.1">
        <property type="nucleotide sequence ID" value="XM_003564932.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUS7"/>
    <dbReference type="STRING" id="15368.I1HUS7"/>
    <dbReference type="EnsemblPlants" id="KQK11314">
        <property type="protein sequence ID" value="KQK11314"/>
        <property type="gene ID" value="BRADI_2g59410v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832428"/>
    <dbReference type="Gramene" id="KQK11314">
        <property type="protein sequence ID" value="KQK11314"/>
        <property type="gene ID" value="BRADI_2g59410v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832428"/>
    <dbReference type="eggNOG" id="KOG1021">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039682_1_0_1"/>
    <dbReference type="OMA" id="CQPKIYV"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HUS7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010417">
        <property type="term" value="P:glucuronoxylan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF200">
        <property type="entry name" value="BETA-1,4-XYLOSYLTRANSFERASE IRX10L-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094875" description="Exostosin GT47 domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="51"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK11314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="418" mass="46724" checksum="F314D517E9A2DA06" modified="2012-06-13" version="1">MARWGLAAIAVLVAAAALFVAAEAQAAQQGHQTERISGSAGDVLDDNPVGRLKVYIYDLPGKYNKKLLKKDPRCLNHMFAAEIFMHRFLLSSAVRTFNPEEADWFYTPVYATCDLTPSGLPLPFKSPRMVRSAIQLIAEKWPYWNRSEGADHFFVAPHDFGACFHYQEEKAIGRGILPLLQRATLVQTFGQKNHVCLKDGSITIPPYAPPQKMQNHLIPGDTPRSIFVYFRGLFYDTSNDPEGGYYARGARASVWENFKNNPLFDISTDHPPTYYEDMQRSVFCLCPLGWAPWSPRLVEAVVFGCIPVIIADDIVLPFADAIPWEDIGVFVSEDDVPKLDSILTSIPTDVILRKQRLLANPSMKQAMLFPQPAEAGDAFHQILNGLARKLPHGENVFLKPGQKVLNWTAGPVGDLKPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUU9</accession>
    <name>I1HUU9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100828986</name>
        <name evidence="1" type="ORF">BRADI_2g59630v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567430.1">
        <property type="nucleotide sequence ID" value="XM_003567382.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUU9"/>
    <dbReference type="STRING" id="15368.I1HUU9"/>
    <dbReference type="EnsemblPlants" id="KQK11348">
        <property type="protein sequence ID" value="KQK11348"/>
        <property type="gene ID" value="BRADI_2g59630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828986"/>
    <dbReference type="Gramene" id="KQK11348">
        <property type="protein sequence ID" value="KQK11348"/>
        <property type="gene ID" value="BRADI_2g59630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828986"/>
    <dbReference type="eggNOG" id="ENOG502R8YE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084347_1_0_1"/>
    <dbReference type="OMA" id="QRIEYYG"/>
    <dbReference type="OrthoDB" id="1512693at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33265">
        <property type="entry name" value="AVR9/CF-9 RAPIDLY ELICITED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33265:SF6">
        <property type="entry name" value="OS01G0930500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK11348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="224" mass="24330" checksum="F4806FBEA0E38CAB" modified="2012-06-13" version="1">MDVQSAPGAAKRMWSYLRAVFLMLRKGKRRLLLGLHLLMKRRNNKGALARSVATLLSHSHHGHGYGHAHALSHRRRGEYEFSCSGSPVDPRRRSNAYFPCLLGSEAETAPTAALQYQYRIEYDNYAASTAAAEAAPEEERDGVLMEELAAGEDECGSTSAESVPSPLVASAGGFSVRVSNFSSEDGGGGGEAVDEEAEEFISRFYEQLRQQNQIALSAAPVPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXA3</accession>
    <name>I1HXA3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100836271</name>
        <name evidence="18" type="ORF">BRADI_3g04040v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ADAGIO family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574962.1">
        <property type="nucleotide sequence ID" value="XM_003574914.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXA3"/>
    <dbReference type="FunCoup" id="I1HXA3">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HXA3"/>
    <dbReference type="EnsemblPlants" id="KQJ93355">
        <property type="protein sequence ID" value="KQJ93355"/>
        <property type="gene ID" value="BRADI_3g04040v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93356">
        <property type="protein sequence ID" value="KQJ93356"/>
        <property type="gene ID" value="BRADI_3g04040v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93357">
        <property type="protein sequence ID" value="KQJ93357"/>
        <property type="gene ID" value="BRADI_3g04040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836271"/>
    <dbReference type="Gramene" id="KQJ93355">
        <property type="protein sequence ID" value="KQJ93355"/>
        <property type="gene ID" value="BRADI_3g04040v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93356">
        <property type="protein sequence ID" value="KQJ93356"/>
        <property type="gene ID" value="BRADI_3g04040v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93357">
        <property type="protein sequence ID" value="KQJ93357"/>
        <property type="gene ID" value="BRADI_3g04040v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836271"/>
    <dbReference type="eggNOG" id="ENOG502QQ74">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033494_1_0_1"/>
    <dbReference type="OMA" id="CITGSRM"/>
    <dbReference type="OrthoDB" id="447251at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22154">
        <property type="entry name" value="F-box_AtADO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000021">
        <property type="entry name" value="Adagio protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000041">
        <property type="entry name" value="Adagio protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000005">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000062">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011498">
        <property type="entry name" value="Kelch_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46175:SF1">
        <property type="entry name" value="ADAGIO-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46175">
        <property type="entry name" value="BACTERIOOPSIN TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07646">
        <property type="entry name" value="Kelch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="11" id="KW-0157">Chromophore</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="PAS" evidence="17">
        <location>
            <begin position="61"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQJ93355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="617" mass="66558" checksum="07C070E310420D5A" modified="2012-06-13" version="1">MEWDSDSDGGSGDEEEEEEGVGPWGGGGGGGGAGFSLAIEGVLGGACGLVVSDALEPDFPIIYVNRGFEDATGYHAEEVLGRNCRFLQCRGPFAQRRHPLVDAIVVAEIQRCLEEGTEFQGDLLNFRKDGSPFMTNLQLKPIYGDDETITHYMGIQFFNDSNVDLGPLPGSMTKDAVRSIWIAPDNTHRPSPTGKGNFCSEHSNLFQLSDEVLCQKILSRLSPRDIASVNSVCKRLYHMTKNDHLWRMVCQNAWGSEATRALENVAGSKSLAWGRIAREMTTLEAVAWRKLTIGGTVEPSRCNFSACAVGNRVVLFGGEGVNMQPMNDTFVLDLSASKPEWRHINVGLAPPGRWGHTLSCLSGSLLVLFGGCGGQGLLNDVFILDLDAKHPTWREILGLAPPVPRSWHSSCTLDGSKLVVSGGCADSGVLLSDTYLLDVTMERPVWRLIPAPWTPPSRLGHSLSVYDGRKILMFGGLAKSGPLRLRSGDVFTMDLSEAVPSWRCITGSGMPGACNPAGVGPPPRLDHVAVSLPGGRILIFGGSVAGLHSASQLYILDPTEEKPTWRILNVPGRPPRFAWGHSTCVMEGTKAIVLGGQTGEEWTLTEIHELSLTSSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXB2</accession>
    <name>I1HXB2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">T-complex protein 1 subunit zeta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100833189</name>
        <name evidence="9" type="ORF">BRADI_3g04130v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574787.1">
        <property type="nucleotide sequence ID" value="XM_003574739.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXB2"/>
    <dbReference type="FunCoup" id="I1HXB2">
        <property type="interactions" value="2647"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HXB2"/>
    <dbReference type="EnsemblPlants" id="KQJ93371">
        <property type="protein sequence ID" value="KQJ93371"/>
        <property type="gene ID" value="BRADI_3g04130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833189"/>
    <dbReference type="Gramene" id="KQJ93371">
        <property type="protein sequence ID" value="KQJ93371"/>
        <property type="gene ID" value="BRADI_3g04130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833189"/>
    <dbReference type="eggNOG" id="KOG0359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_3_1_1"/>
    <dbReference type="OMA" id="LHPRIMT"/>
    <dbReference type="OrthoDB" id="10052040at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03342">
        <property type="entry name" value="TCP1_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000058">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000004">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012722">
        <property type="entry name" value="Chap_CCT_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02347">
        <property type="entry name" value="chap_CCT_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ93371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="59243" checksum="08EA1871E4988D93" modified="2012-06-13" version="1">MSLRVLNPNAEVLNKSAALHMNINAAKGLQDVLRTNLGPKGTIKMLVGGAGDIKLTKDGNTLLKEMQIQNPTAIMIARTAVAQDDTSGDGTTSTVLFIGELMKQSERCIEEGTHPRFLVDGFEVAKRATLEFLEKFKTPVVMGDEPDREMLKMVARTTLRTKLYEGLADQLTDIVLNAVLCVRQKDEPIDLFMVEIMHMRHKFDVDTHLIEGLVLDHGSRHPDMKRRAENCYILTANVSLEYEKSEINAGFFYSNAEQREKMVSAERRQVDERVKKIIELKNKVCSGTDKNFVVINQKGIDPPSLDLLARAGIIALRRAKRRNMERLVLACGGEAINSVDDLTEDCLGWAGLVYEHVLGEEKYTFVENVKNPHSCTILIKGPNDHTIAQIKDAVRDGLRSVKNTVEDEAVVLGAGAFEMAAKKHLMDNVKKTVKGRAQLGVAAFADALLVIPKTLAENSGLDTQDVIVALENEHDRGLVVGLNHHSGEPVDPQMEGIFDNYSVKRQIINSGPIIASQLLLVDEVIRAGRNMRKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXT5</accession>
    <name>I1HXT5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100835109</name>
        <name evidence="10" type="ORF">BRADI_3g05577v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570405.1">
        <property type="nucleotide sequence ID" value="XM_003570357.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXT5"/>
    <dbReference type="FunCoup" id="I1HXT5">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HXT5"/>
    <dbReference type="EnsemblPlants" id="KQJ93591">
        <property type="protein sequence ID" value="KQJ93591"/>
        <property type="gene ID" value="BRADI_3g05577v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835109"/>
    <dbReference type="Gramene" id="KQJ93591">
        <property type="protein sequence ID" value="KQJ93591"/>
        <property type="gene ID" value="BRADI_3g05577v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835109"/>
    <dbReference type="eggNOG" id="ENOG502QV87">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057781_0_0_1"/>
    <dbReference type="OMA" id="VWGWDSN"/>
    <dbReference type="OrthoDB" id="1299653at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14702">
        <property type="entry name" value="bZIP_plant_GBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000020">
        <property type="entry name" value="BZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045314">
        <property type="entry name" value="bZIP_plant_GBF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044168">
        <property type="entry name" value="RISBZ3/4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47693:SF1">
        <property type="entry name" value="BZIP TRANSCRIPTION FACTOR RISBZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47693">
        <property type="entry name" value="BZIP TRANSCRIPTION FACTOR RISBZ3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="143"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="154"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ93591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="312" mass="32413" checksum="C4D94B5B92E34BF1" modified="2012-06-13" version="1">MKKCASELELEAFIRGRGAAAAAVAEQKPGHAAAAAAGTHGPFGVFSAADLAGFGFADSNTLNGGIHNHLWSQSPNLGARLPAVSTTIDSQSSIYAAASPTSATNLSMKENQGFGGTSGSDSDSESMFDMEGGLCDQSTNPTDVKRMRRMVSNRESARRSRKRKQAHLVELETQVDQLRGDNASIFKQLTDANQQFTTAVTDNRILKSDVEALRAKVKLAEKMVSQGALSCGLGHLGLSPAALVPDGLAGLDFLPGGGADDACFASLSPAEQVQGSPMQSMASLESLDHGGRMPGGGDVWGWDSNSNGALSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYN1</accession>
    <name>I1HYN1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100835421</name>
        <name evidence="7" type="ORF">BRADI_3g07910v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234027.1">
        <property type="nucleotide sequence ID" value="XM_010235725.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317609.1">
        <property type="nucleotide sequence ID" value="XM_024461841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYN1"/>
    <dbReference type="FunCoup" id="I1HYN1">
        <property type="interactions" value="1112"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HYN1"/>
    <dbReference type="EnsemblPlants" id="KQJ93988">
        <property type="protein sequence ID" value="KQJ93988"/>
        <property type="gene ID" value="BRADI_3g07910v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93990">
        <property type="protein sequence ID" value="KQJ93990"/>
        <property type="gene ID" value="BRADI_3g07910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835421"/>
    <dbReference type="Gramene" id="KQJ93988">
        <property type="protein sequence ID" value="KQJ93988"/>
        <property type="gene ID" value="BRADI_3g07910v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93990">
        <property type="protein sequence ID" value="KQJ93990"/>
        <property type="gene ID" value="BRADI_3g07910v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835421"/>
    <dbReference type="eggNOG" id="KOG0702">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021712_0_0_1"/>
    <dbReference type="OMA" id="PMPHASH"/>
    <dbReference type="OrthoDB" id="6036at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HYN1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08838">
        <property type="entry name" value="ArfGap_AGFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000005">
        <property type="entry name" value="Arf-GAP domain and FG repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044820">
        <property type="entry name" value="AGD14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46085:SF3">
        <property type="entry name" value="ARF GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46085">
        <property type="entry name" value="ARFGAP/RECO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="6">
        <location>
            <begin position="12"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="123"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="479"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="575"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="659"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="163"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
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        <location>
            <begin position="292"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="323"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="384"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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            <begin position="407"/>
            <end position="420"/>
        </location>
    </feature>
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        <location>
            <begin position="430"/>
            <end position="442"/>
        </location>
    </feature>
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        <location>
            <begin position="483"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ93990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="704" mass="76064" checksum="EA355DE07681E748" modified="2012-06-13" version="1">MASRVKEDERNERVIRGLLKLPANKRCINCNNLGPQYVCTNFWTFVCTNCSGAHREFTHRVKSVSMAKFTAQEVSALQGGGNERAREIFFKEWDSQRGSYPDSSNVDKLRNFIKHVYVERRYTGERSSDRPPRGKDDKDEPSENRRSDGNRGGSRSPPYNESYSDRRSYSGRSDDRNSRYSYGERSPGYDQSDYKKSPRCFEAVDDRSGKTTPVQRFEDRRFSEPRKPETGSPNYEREANGSSPPVVRPVREILGDDAPQLRIGEPPKPNVAKQIDPPKPNGIRTIEPPPQAQRTSTATSVGSSEGTLEQMKVASPISLIDFSADPEPSASAPPSQTGPAPQQHPVNAQPPQPINAAVQQPVLEQGKSAPSVSGGDWASFDAFGQQQIPQATSSVNPLESVLAELSFSETPSAPNTSTFPISVDPKANDGGQSSMIDQSHSSLFGAPLGISGNQHQASTGMSIQGSSIQQSAVAAPMGVLPSQVPSSSQGTSGIQEATSSADDKSSGRKPLPVDFFTSLYPSAAPTMPGWQRAPQFGMGFAMQYPPGMLQGGYPQAAFSQPAYQQTYQQPLYQQPAYPQHSYPQPAKASNPFDLGNEPAQAHTPLSGPLGASAGATPETLLGTSSFGVPPRQPQQLYQSSAPQNHYMMQHVPNNMPGQLPNNMLPRQQGGPGSLGIGYDQQAAPRYSQPSTPPSYGAVGGNPFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZS4</accession>
    <name>I1HZS4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Oligosaccharyltransferase complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100822454</name>
        <name evidence="7" type="ORF">BRADI_3g11024v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OSTC family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_014900034.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZS4"/>
    <dbReference type="STRING" id="15368.I1HZS4"/>
    <dbReference type="EnsemblPlants" id="PNT66397">
        <property type="protein sequence ID" value="PNT66397"/>
        <property type="gene ID" value="BRADI_3g11024v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822454"/>
    <dbReference type="Gramene" id="PNT66397">
        <property type="protein sequence ID" value="PNT66397"/>
        <property type="gene ID" value="BRADI_3g11024v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822454"/>
    <dbReference type="eggNOG" id="KOG3356">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109136_0_0_1"/>
    <dbReference type="OMA" id="CWIFMRM"/>
    <dbReference type="OrthoDB" id="10256333at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021149">
        <property type="entry name" value="OligosaccharylTrfase_OST3/OST6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042416">
        <property type="entry name" value="OSTC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13160">
        <property type="entry name" value="OLIGOSACCHARYLTRANSFERASE COMPLEX SUBUNIT OSTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13160:SF4">
        <property type="entry name" value="OLIGOSACCHARYLTRANSFERASE COMPLEX SUBUNIT OSTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04756">
        <property type="entry name" value="OST3_OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT66397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="18868" checksum="4D7452031CA616D4" modified="2012-06-13" version="1">MAPRSEHAAGSAAAQVHGSEFESMDPLFHVLRVVPFSFLQPPRTRLKVPSNLALPSPMTVFSLILLTYFAVVSGLVYDVIVEPPGIGSSQDPATGAVRPVVFLPGRVNGQYIIEGLSSGFMFLLGGVGIILLDLAVDRTRPRSLRISFAGSGIAAVVIAYAMAMLFLRIKIPGYLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1I026</accession>
    <name>I1I026_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100839222</name>
        <name evidence="15" type="ORF">BRADI_3g12540v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE associated with ER-derived vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the BET1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573218.1">
        <property type="nucleotide sequence ID" value="XM_003573170.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I026"/>
    <dbReference type="STRING" id="15368.I1I026"/>
    <dbReference type="EnsemblPlants" id="KQJ94682">
        <property type="protein sequence ID" value="KQJ94682"/>
        <property type="gene ID" value="BRADI_3g12540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839222"/>
    <dbReference type="Gramene" id="KQJ94682">
        <property type="protein sequence ID" value="KQJ94682"/>
        <property type="gene ID" value="BRADI_3g12540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839222"/>
    <dbReference type="eggNOG" id="KOG3385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086133_0_0_1"/>
    <dbReference type="OMA" id="HDEVENH"/>
    <dbReference type="OrthoDB" id="261831at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000033">
        <property type="entry name" value="bet1-like SNARE 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="32"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQJ94682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="122" mass="14085" checksum="6DA7B4E567491867" modified="2012-06-13" version="1">MNSRRDFRSHRAALFDGIEEGGIRGSAYSSREIHEHENDQAVDNLHERVSILKRLTGDIHDEVENHNRMLDRMGNDMDTSRGFLSGTVDKFKMVFETKSSRRMATMVASFVAAFLLLYYLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1I048</accession>
    <name>I1I048_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100831856</name>
        <name evidence="11" type="ORF">BRADI_3g12680v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571275.1">
        <property type="nucleotide sequence ID" value="XM_003571227.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I048"/>
    <dbReference type="FunCoup" id="I1I048">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I048"/>
    <dbReference type="EnsemblPlants" id="KQJ94703">
        <property type="protein sequence ID" value="KQJ94703"/>
        <property type="gene ID" value="BRADI_3g12680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831856"/>
    <dbReference type="Gramene" id="KQJ94703">
        <property type="protein sequence ID" value="KQJ94703"/>
        <property type="gene ID" value="BRADI_3g12680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831856"/>
    <dbReference type="eggNOG" id="ENOG502S49Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112618_0_0_1"/>
    <dbReference type="OMA" id="ANSDYYY"/>
    <dbReference type="OrthoDB" id="550883at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31241">
        <property type="entry name" value="DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31241:SF62">
        <property type="entry name" value="DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="40"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ94703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="213" mass="22859" checksum="64B2993CFA39AFDA" modified="2012-06-13" version="1">MAERAASRGRQGNSRKCCPLRRSRKGCMKGKGGPENQRCPFRGVRQRTWGKWVAEIREPNRGARLWLGTFSTALDAARAYDSAAKALYGDCARLNLAGGTNNPVLAPPATAAATSSETQSYSSNSSSGANSDYYYYYNEYLNGNNGGGAWISSAAPPAVEDEDFDTYVRRLPKAEDFGLQAFLQNMPFDVLAEASGTAAGAGIWEPSCDMAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1G5</accession>
    <name>I1I1G5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100840028</name>
        <name evidence="4" type="ORF">BRADI_3g16620v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
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    <dbReference type="EnsemblPlants" id="KQJ95340">
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        <property type="gene ID" value="BRADI_3g16620v3"/>
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        <property type="gene ID" value="BRADI_3g16620v3"/>
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    <dbReference type="eggNOG" id="KOG1790">
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ95340"/>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
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            <taxon>Poaceae</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">Bd21</strain>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ96046.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="152" mass="17137" checksum="6B84E2DF1A76ECD5" modified="2012-06-13" version="1">MDLLLLLSLLSKVGLGLMERAWHGTLPCASPQERMPLALISSPSLFYVKAMHKRLFKASWRVKTALSLANAERRKNAEDAPAGSEDKESDLEYYEHPFSSDEDEEEDVESAYQGRISGRRLQLWLRVSLVPTEQSRSSTRKLVRNLGTAVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1I599</accession>
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        <recommendedName>
            <fullName evidence="8">Myb-like domain-containing protein</fullName>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
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        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I599"/>
    <dbReference type="STRING" id="15368.I1I599"/>
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        <property type="gene ID" value="BRADI_3g30457v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g30457v3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
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        </location>
    </feature>
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    </feature>
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    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="355"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="399"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="578"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="652"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="677"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="715"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ97388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="769" mass="82085" checksum="D584BCD8C4F0ED9D" modified="2012-06-13" version="1">MQQQQGGGGGPGQQFGLHPPEMPPFSPAGQRISMAEAPSPISSRPPAPPGQQQLSSNELAGAAAAMSFDEEALAAGEEGGGGGSGGNRWPRQETLVLLKIRSDMDAAFRDATLKGPLWEEVSRKLAEEGYRRNAKKCKEKFENVHKYYKRTKDSRAGRNDGKTYRFFQQLEALQGATPGAGASSVPPPATAVRAPAEPPPQPVVAGAMPTPMGVGNLSFSTSNTEEFSEDEDEEDDSDDEGTDDMAVVGNKRKRMSSDGVAAAGGHNNKKMMRFFEGLMRQVMERQEAMQQRFLEAIEKREQDRMIREEAWRRQEMARLAREQETLAQERAMAASRDAAVLGFIQKITGQSVPMPMAPPPPSIAFMPPPPAGSHPTPISFSAAPPSSSQSPATQASPRPQKPPMPLPTPAPQKTPVPATPPPQQQSGGMEMVVSAPAGGELQLHDGGSGSASSSRWPKAEVHALIQLRSNLDTRYQEAGPKGPLWEEISAGMRRMGYSRSSKRCKEKWENINKYFKKVKESNKKRPEDSKTCPYFHQLEALYRNKQQAALTSPSAAAAPLPALAAPPPPEPFTVAAPISQTPPTTTHALQQPAAAKNGAGNGHGNGNGSGVAACSEGGSVSAGGMQASNGFFGEAGVAAKKPEGMMKETIMEQRQQAQAAPISSSYNNRAGVVDSDNSMDDEDDYDDEDEEDDDDDDVDGNKMQYEIQFQSQQHHQLHQQHHQHNNHNVVRPNAGAAGGGNQPGAAAPPSAASAAAATTTAGSFLGMVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6R1</accession>
    <name>I1I6R1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100836564</name>
        <name evidence="5" type="ORF">BRADI_3g34990v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572159.1">
        <property type="nucleotide sequence ID" value="XM_003572111.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6R1"/>
    <dbReference type="STRING" id="15368.I1I6R1"/>
    <dbReference type="EnsemblPlants" id="KQJ98117">
        <property type="protein sequence ID" value="KQJ98117"/>
        <property type="gene ID" value="BRADI_3g34990v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836564"/>
    <dbReference type="Gramene" id="KQJ98117">
        <property type="protein sequence ID" value="KQJ98117"/>
        <property type="gene ID" value="BRADI_3g34990v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836564"/>
    <dbReference type="eggNOG" id="KOG1616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="GLHTCRP"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I6R1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043036">
        <property type="term" value="C:starch grain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019203">
        <property type="term" value="F:carbohydrate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005983">
        <property type="term" value="P:starch catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14526">
        <property type="entry name" value="DSP_laforin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000072">
        <property type="entry name" value="Phosphoglucan phosphatase LSF1 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000972">
        <property type="entry name" value="Phosphoglucan phosphatase LSF1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045204">
        <property type="entry name" value="DSP_laforin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE LSF1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661:SF2">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE LSF1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="300"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ98117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="603" mass="67311" checksum="759AD12093BB4CC3" modified="2012-06-13" version="1">MALHLVPAPNIALLAAPSAAGAATGVPGGVRVRSVARPSASRVRASAAWWTGRRSGWRRRLGVAVAMAGGETEDEPEEAVATVRMNLNEYMVAVDRPLGVRFALAVNGRVFVHSLKRGGNAEKSRIIMVGDTLKKAGQSESLVNIKDLGDTEMALKEKSGPCNLVLERPFAPYPIHQLHQNEDYHILFNRGRVALATWNSAQLSSKLNESSPGNGNSGFAMFCPRLLSAQGWSLLSREKDGLNRKSTNLANRISEIVGLYSDEDDLDAEWAHGSFPLEEYIKALDRAKGELYYNHSLGMQYSKITEQIFVGSCIQTEKDVKMLSETVGITAVLNFQSESERINWGINSEAINSSCRENNILMINYPIREVDSMDLRKKLPFCVGLLLRLIRKNYRIYVTCTTGYDRSPACVIAYLHWVQDTPLHIAHKFITGLHSCRPDRAAIVWATWDLIQLVENGRHDGTPTHSVCFVWNNGREGEEVELIGDFTSNWKDKLKCNHQGGSRHEAEVRLRHGKYYYKFIVGGQWRHSTSLPSETDEHGNVNNVIRVGDIARIRPAPSQLQIRDPTVVKVIERALTEDERFSLAFAARRMAFAICPIRLAPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6U2</accession>
    <name>I1I6U2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104583707</name>
        <name evidence="3" type="ORF">BRADI_3g35240v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235104.1">
        <property type="nucleotide sequence ID" value="XM_010236802.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235105.1">
        <property type="nucleotide sequence ID" value="XM_010236803.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6U2"/>
    <dbReference type="STRING" id="15368.I1I6U2"/>
    <dbReference type="EnsemblPlants" id="KQJ98166">
        <property type="protein sequence ID" value="KQJ98166"/>
        <property type="gene ID" value="BRADI_3g35240v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68037">
        <property type="protein sequence ID" value="PNT68037"/>
        <property type="gene ID" value="BRADI_3g35240v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583707"/>
    <dbReference type="Gramene" id="KQJ98166">
        <property type="protein sequence ID" value="KQJ98166"/>
        <property type="gene ID" value="BRADI_3g35240v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68037">
        <property type="protein sequence ID" value="PNT68037"/>
        <property type="gene ID" value="BRADI_3g35240v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583707"/>
    <dbReference type="eggNOG" id="ENOG502QUAU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056082_1_0_1"/>
    <dbReference type="OMA" id="KVKHRCS"/>
    <dbReference type="OrthoDB" id="609232at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46477">
        <property type="entry name" value="CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46477:SF8">
        <property type="entry name" value="OS08G0257100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DC1" evidence="2">
        <location>
            <begin position="18"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ98166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="268" mass="28931" checksum="A3383A2FF049D126" modified="2012-06-13" version="1">MTILSTGDAAEIFLPAVHLHKLKKTEPAGDVNRFKCDGCKEDNGAGVRYTCTSPACNFDLHTCCAKLAGARLVHPFDKSIELKFLYEAPTSENDRRLCDACGGKVLGFNYQCGNLNLHPCCAELPALIEKEDLAFELREKASHKCTGCGGSGGYMTFWFYRSKCKKYYLHVRCVKEILLGQQLNQLAAAAGSSSSSSSSSSAAVAAGDVVTTFVKERALEKYKKKMDGETFERILRVVATVLRVILGVLIGDPTTLLIAGAAFLPGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6Z7</accession>
    <name>I1I6Z7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100839118</name>
        <name evidence="9" type="ORF">BRADI_3g35720v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574474.1">
        <property type="nucleotide sequence ID" value="XM_003574426.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6Z7"/>
    <dbReference type="FunCoup" id="I1I6Z7">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ98246">
        <property type="protein sequence ID" value="KQJ98246"/>
        <property type="gene ID" value="BRADI_3g35720v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839118"/>
    <dbReference type="Gramene" id="KQJ98246">
        <property type="protein sequence ID" value="KQJ98246"/>
        <property type="gene ID" value="BRADI_3g35720v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839118"/>
    <dbReference type="eggNOG" id="KOG2640">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051582_2_0_1"/>
    <dbReference type="OMA" id="KQAWRMR"/>
    <dbReference type="OrthoDB" id="19690at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I6Z7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd02999">
        <property type="entry name" value="PDI_a_ERp44_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044606">
        <property type="entry name" value="APRL4/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46854:SF2">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46854">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE-LIKE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095080" description="Thioredoxin domain-containing protein" evidence="7">
        <location>
            <begin position="22"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="37"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ98246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="257" mass="28766" checksum="6106BA3222641B5B" modified="2012-06-13" version="1">MSAKASAAALFLFALPLLAAASEGACPKTPAAAAILRQTSGSCPAADSPGLRGHNVGVVEGDDGVLQRAVTLVLQNREDFVAILFYASWCPFSKIFRTDFQKLSSFFPTIAHFSFEESHIKPRMLSRYGVRAFPTLFLVNSTVRVRYHGSRAMNSLVMFYKDVTGINPVSLDAISLERMQDIVNIVENEKKTEQEDSLFLWARSPDRLLHQDTCLAFASTFVILRLFFFLLPKLNACVKQAWRMRLHELKRLFPSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1I782</accession>
    <name>I1I782_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100832782</name>
        <name evidence="2" type="ORF">BRADI_3g36407v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572217.1">
        <property type="nucleotide sequence ID" value="XM_003572169.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I782"/>
    <dbReference type="STRING" id="15368.I1I782"/>
    <dbReference type="EnsemblPlants" id="KQJ98351">
        <property type="protein sequence ID" value="KQJ98351"/>
        <property type="gene ID" value="BRADI_3g36407v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832782"/>
    <dbReference type="Gramene" id="KQJ98351">
        <property type="protein sequence ID" value="KQJ98351"/>
        <property type="gene ID" value="BRADI_3g36407v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832782"/>
    <dbReference type="eggNOG" id="ENOG502RP95">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083135_1_0_1"/>
    <dbReference type="OMA" id="NEGEICL"/>
    <dbReference type="OrthoDB" id="676555at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33148:SF3">
        <property type="entry name" value="DUF4228 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33148">
        <property type="entry name" value="PLASTID MOVEMENT IMPAIRED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ98351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="260" mass="28047" checksum="59079654DB566EEF" modified="2012-06-13" version="1">MGNAVGGMKRRKTARVMTVDGTTYKYRPPAAACAALREHPGHQLLESEDVRRLGVRARPLDADAPLKPGKLYFLVALPRLAARARAPQRTWSGALLGGSGSCAGERLERLMLSSSSRRSASDVAAAVASPMWRRSASDVAAAVASPLSASVEAAADGAVRLRMRLPKAEVARLLEESKDADEAAERIMRLCVDRDQYCYRSAPATPLLPLPMPMPMPMPLPLPLPALASSDKSKKHAADGGNKKEKRARFEAVPDEIIWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I1IE84</accession>
    <name>I1IE84_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100822335</name>
        <name evidence="1" type="ORF">BRADI_3g56132v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317557.1">
        <property type="nucleotide sequence ID" value="XM_024461789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IE84"/>
    <dbReference type="EnsemblPlants" id="KQK01485">
        <property type="protein sequence ID" value="KQK01485"/>
        <property type="gene ID" value="BRADI_3g56132v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822335"/>
    <dbReference type="Gramene" id="KQK01485">
        <property type="protein sequence ID" value="KQK01485"/>
        <property type="gene ID" value="BRADI_3g56132v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2281328_0_0_1"/>
    <dbReference type="OMA" id="CATFYRI"/>
    <dbReference type="OrthoDB" id="690492at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31264:SF10">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31264">
        <property type="entry name" value="OS07G0554500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK01485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11154" checksum="CB09AFDDD85549E2" modified="2012-06-13" version="1">MAQSTLQAPAPEQLTLDELLEEIFLRLPTAADLARAAMACVSFRRVIAGHRFLRRFRVLQPPPLLGMLEGIINLPFRPALPPHPSAAAARAFADADPDLLCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IE86</accession>
    <name>I1IE86_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100830123</name>
        <name evidence="11" type="ORF">BRADI_3g56150v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572978.1">
        <property type="nucleotide sequence ID" value="XM_003572930.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IE86"/>
    <dbReference type="EnsemblPlants" id="KQK01487">
        <property type="protein sequence ID" value="KQK01487"/>
        <property type="gene ID" value="BRADI_3g56150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830123"/>
    <dbReference type="Gramene" id="KQK01487">
        <property type="protein sequence ID" value="KQK01487"/>
        <property type="gene ID" value="BRADI_3g56150v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830123"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013137_15_5_1"/>
    <dbReference type="OMA" id="PCHECKE"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF25">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="84"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK01487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="153" mass="15972" checksum="8292F25FCCAC5CE7" modified="2012-06-13" version="1">MATGILSTVLLVAGVTLIVLVNILVVVWVLRRGFIARRLSRVGERADQEGGAVGGLTEEEVGELPCRDFKPDQLAAGELGGGECAVCLEALKDGERCAVLPRCGHGFHADCVGSWLRKSRLCPVCRAEVAAGSPRKEAGAVAAHDAEAAVEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IFG1</accession>
    <name>I1IFG1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TORTIFOLIA1/SINE1-2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100841641</name>
        <name evidence="3" type="ORF">BRADI_3g59730v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570678.1">
        <property type="nucleotide sequence ID" value="XM_003570630.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IFG1"/>
    <dbReference type="FunCoup" id="I1IFG1">
        <property type="interactions" value="2137"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IFG1"/>
    <dbReference type="EnsemblPlants" id="KQK01998">
        <property type="protein sequence ID" value="KQK01998"/>
        <property type="gene ID" value="BRADI_3g59730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841641"/>
    <dbReference type="Gramene" id="KQK01998">
        <property type="protein sequence ID" value="KQK01998"/>
        <property type="gene ID" value="BRADI_3g59730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841641"/>
    <dbReference type="eggNOG" id="ENOG502QQTY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025475_0_0_1"/>
    <dbReference type="OMA" id="LNHKNWD"/>
    <dbReference type="OrthoDB" id="1904066at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000586">
        <property type="entry name" value="Microtubule-associated protein TORTIFOLIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033337">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057600">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN TORTIFOLIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355:SF29">
        <property type="entry name" value="OS02G0739900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24714">
        <property type="entry name" value="TOR1L1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TORTIFOLIA1/SINE1-2 N-terminal" evidence="2">
        <location>
            <begin position="16"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK01998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="622" mass="66717" checksum="F6BB77A390F5AF80" modified="2012-06-13" version="1">MGTAARPRGAAAAEPLKQRVNRCLLRLSDRDTEAMAAAELDAIARTLGADELAAFVSAVSDARPTDKAPLRRHTLRLLALLAASQPRDAVAPLVPRILAAALRRVRDQDSSVRAALVDAARSSAAAASSASAALSPLADALLHEQDQCAQLAAALAMAAAVEASALTADLASYLHRLQPRLLKLLRSNAFKAKPALITLIGASAAVAGAAEVTASIPCLRDAIGSDDWAARKAAAEALAALALEYKDLLLTYKSSCIAYFEARRFDKVKIVRESMSRMIEAWKEIPDVEEEEFSSCTAPVSQSQRRSSVAGSASGGGYPTASLGSNSVQSATRRNRPPTGRSPPPDVSPGAAKRHSPSSIRNKKLSPPSYRKVGQAKSSGYKDEIAIAPDATPIKEVTEEKLLKGNNTRSRLESRRALFQGSEERTAKLAGQRAGSRVVPYEGGNLEEISEVEGGPERFQSGHKDEGLLEIKSQLLQIEKQQSSLLDLLQKFMGKSENGMNSLETRVHGLEMALDEISRDLAVSSERMSNSEPRLNSCCILSPKFWRRHDGGRNSSRFSSSDVPNSSEGSRTSYKWERQKFGVQGGFITNPLAEPNISSVGGTVVTQEGRRKELALQKSRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMZ6</accession>
    <name>I1IMZ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100831479</name>
        <name evidence="11" type="ORF">BRADI_4g23880v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577770.1">
        <property type="nucleotide sequence ID" value="XM_003577722.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237885.1">
        <property type="nucleotide sequence ID" value="XM_010239583.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IMZ6"/>
    <dbReference type="FunCoup" id="I1IMZ6">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89164">
        <property type="protein sequence ID" value="KQJ89164"/>
        <property type="gene ID" value="BRADI_4g23880v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT64054">
        <property type="protein sequence ID" value="PNT64054"/>
        <property type="gene ID" value="BRADI_4g23880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831479"/>
    <dbReference type="Gramene" id="KQJ89164">
        <property type="protein sequence ID" value="KQJ89164"/>
        <property type="gene ID" value="BRADI_4g23880v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64054">
        <property type="protein sequence ID" value="PNT64054"/>
        <property type="gene ID" value="BRADI_4g23880v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831479"/>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000837_14_1_1"/>
    <dbReference type="OMA" id="FICEYII"/>
    <dbReference type="OrthoDB" id="693153at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1135">
        <property type="entry name" value="OS08G0246300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="184"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="414"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="654"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="779"/>
            <end position="1132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT64054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1154" mass="130224" checksum="1CF49A7B28BC2AF7" modified="2012-06-13" version="1">MGDLVIGLAKSVVEGTLSKAQSAIEEHGKLRQQAQRDLVFITFEFEMMHAFLSVADQESAAKNTVVRTWVRHVRELAYDVEDCIELVIHLDNKSDWWRRLLPPCIAAAALPLDQAVAEIEQLKARVVDVSSSYMRYNLVSDFGSKSTVAPSAAVGATAPNLVMKAKEDVEGLREFRDLARLINMKKHDLQVISVWGTGSHLGITSIIRKAYNDPEICPNFACQAWVKLIHPFNPQEFIQCLMAQFYANSCEKQGAIVGLQVLQKMEATQENLLKEFANQINGNRYLVVLEGLSSMVEWDAIRTFLPDRKNGSWIIASTEQFEMASLCVAHPYKVLELKQFSAEHSVCAFLTEASQGDGDKGKEISEEHEICSFDKILNGNKDAAIDWMENSLFVGREPEIDKLCQYAAMARFNPYQVISVWGIAGVGKSALVKKFYYDRVLDSSHFEKFSWVDVCHPLNLTDFSRSLLLDLYSEEDPIKECRQLLEHSNCLVVIDNLQSTEEWDLIQAALVSRSSKSLIIVITTEASIATYCAGRGGLVFNVKCLEPEAAFDLLKNEVHTKNSWQPLNDLKEDAELSELILKCGGIPKVIVGIARLLATKTVTLMDTISSLNHRFMYELETNPEFDLHGLFGWMHSFFRSCPDPLKPCIFYLSVFPRDHAVRRRRLVRRWIAEGYSRDTKDISADESGEKFFSKLIDMSVIQQPPQTVATVFDETRMVSCQVKGFICEYIISQKTEENLDFDLGGRCTMTTQRTGRHLAIWDSWDRDKIVFESIDFSRLRSMTVYGKWKPFLVSKKMKLLRVLDLEDASGVTDDDLEQMVKLLPRLKFLSLRGCIGVSHLPSSLGDLRQLQTLDIRHSSIVNLPENITKLQKLQYIRGGISGPALVKQLSMFGSQRRLVDIVVPRGIMELTALHTLGVVNVSASGGKAILEELEQLTQLRKLGVSGINKRNRLKFFSAISGHCHLESLSVRPDKDSEGCLDGILMPSENLQSLKLHGLLDSLPRGIDLHSKLTKLDLEMTILKRSVIEFLGKLPKICILRLHVKQTEDDSLHFCVYMNGLEDRSYQKVKILKIACSSSLHVTFGSETMRHLELLKISCCSGSSVKVSGLENLTELKEVLIKGSCEDTMEEDLRRQFDDRRRPPVFKLEEPTASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPN0</accession>
    <name>I1IPN0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100836698</name>
        <name evidence="7" type="ORF">BRADI_4g28877v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578032.1">
        <property type="nucleotide sequence ID" value="XM_003577984.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPN0"/>
    <dbReference type="FunCoup" id="I1IPN0">
        <property type="interactions" value="3022"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IPN0"/>
    <dbReference type="EnsemblPlants" id="KQJ89998">
        <property type="protein sequence ID" value="KQJ89998"/>
        <property type="gene ID" value="BRADI_4g28877v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836698"/>
    <dbReference type="Gramene" id="KQJ89998">
        <property type="protein sequence ID" value="KQJ89998"/>
        <property type="gene ID" value="BRADI_4g28877v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836698"/>
    <dbReference type="eggNOG" id="KOG2476">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019955_2_0_1"/>
    <dbReference type="OMA" id="IVPITHY"/>
    <dbReference type="OrthoDB" id="444325at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IPN0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061632">
        <property type="term" value="F:RNA lariat debranching enzyme activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07380">
        <property type="entry name" value="MPP_CWF19_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000018">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040194">
        <property type="entry name" value="Cwf19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006768">
        <property type="entry name" value="Cwf19-like_C_dom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006767">
        <property type="entry name" value="Cwf19-like_C_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072">
        <property type="entry name" value="CWF19, CELL CYCLE CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072:SF4">
        <property type="entry name" value="CWF19-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04677">
        <property type="entry name" value="CwfJ_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04676">
        <property type="entry name" value="CwfJ_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="313"/>
            <end position="341"/>
        </location>
    </feature>
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        <location>
            <begin position="350"/>
            <end position="373"/>
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    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="313"/>
            <end position="341"/>
        </location>
    </feature>
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        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ89998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="608" mass="66799" checksum="835324F55CE661DC" modified="2012-06-13" version="1">MAAAPPPAPPRILLAGDAHGRLHQLFKRVKSVNQSTGPFHALLCVGQFFSPEAAEGDSLPGDVADYLEGRADVPIPTYFTGDYGPTAPRLLSKAAADARGFSPGGIQLCPNLFWLRGSALFNLHGLSVAYLSGKKGPGGPGCYSQDDVDALRALAEEPGIVDLFLTNEWPTGMVNGADTSNVPPQVLDPQGYDPVVAELVAEIKPRYHIAGTKGVFYAREPYVNDSSPHVTRFIGLANVGNKDKQKFIHAISPTPASTMSSADIHVKPPNTTVSPYLAPAKSVPVEDTTKRPAENTDLQYWRYDVKRQRQGQTNGSLLCFKYTSSGSCPRGSKCNFRHDEEAREHCQRNVCFDFLNKGKCEKGPECRYAHSLSEEGAVRDAKPRSDRRRVESSCWFCLSSPDVESHLVISIGDGYYCALAKGPLVPDHVLVIPVEHFPNTVMMPVESEAELRRYKNALGKYYEKQGKAAVYFEWVSQQSRHANLQAVPVALSKAASVKKIFHLAAQRLGFEFSVVNPDGDANQGRELLRSQYDGKSGLFYVELPEGTLLLHMIDSSEKFPAQFGREVLAGLLSMADRADWRNCKLSKEEEIQMVADFKQGFSEFDPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1IPT6</accession>
    <name>I1IPT6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100845238</name>
        <name evidence="9" type="ORF">BRADI_4g29300v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578058.1">
        <property type="nucleotide sequence ID" value="XM_003578010.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IPT6"/>
    <dbReference type="FunCoup" id="I1IPT6">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IPT6"/>
    <dbReference type="EnsemblPlants" id="KQJ90077">
        <property type="protein sequence ID" value="KQJ90077"/>
        <property type="gene ID" value="BRADI_4g29300v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845238"/>
    <dbReference type="Gramene" id="KQJ90077">
        <property type="protein sequence ID" value="KQJ90077"/>
        <property type="gene ID" value="BRADI_4g29300v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845238"/>
    <dbReference type="eggNOG" id="KOG4431">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087356_4_1_1"/>
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    <dbReference type="OrthoDB" id="6604018at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
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        <property type="entry name" value="RCF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297:SF3">
        <property type="entry name" value="HIG1 DOMAIN FAMILY MEMBER 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297">
        <property type="entry name" value="HYPOXIA-INDUCBILE GENE 1 HIG1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ90077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="99" mass="10433" checksum="8CB4B482D61A696C" modified="2012-06-13" version="1">MEKGSAAGGSSRPPPMPSLEDFPFEGKKPVKNPFVPIGALVTAGVLTAGLVSFRYGNSRLGQKLMRARVVAQGVTVALMVGSAYYYGDQIKLFKKGSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IUP6</accession>
    <name>I1IUP6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U3 small nucleolar ribonucleoprotein protein MPP10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100838045</name>
        <name evidence="9" type="ORF">BRADI_4g43500v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
            <location evidence="1 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the MPP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758081.1">
        <property type="nucleotide sequence ID" value="XM_014902595.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IUP6"/>
    <dbReference type="FunCoup" id="I1IUP6">
        <property type="interactions" value="238"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IUP6"/>
    <dbReference type="EnsemblPlants" id="KQJ92414">
        <property type="protein sequence ID" value="KQJ92414"/>
        <property type="gene ID" value="BRADI_4g43500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838045"/>
    <dbReference type="Gramene" id="KQJ92414">
        <property type="protein sequence ID" value="KQJ92414"/>
        <property type="gene ID" value="BRADI_4g43500v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838045"/>
    <dbReference type="eggNOG" id="KOG2600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011271_5_1_1"/>
    <dbReference type="OMA" id="HFAEDFG"/>
    <dbReference type="OrthoDB" id="445326at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IUP6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012173">
        <property type="entry name" value="Mpp10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17039">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17039:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04006">
        <property type="entry name" value="Mpp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017300">
        <property type="entry name" value="snoRNP_Mpp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 7" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="94"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="394"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="516"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="218"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="409"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="516"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="541"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ92414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="563" mass="62985" checksum="A81DD4623E387348" modified="2012-06-13" version="1">MAVDSGSPLAAEKGEAALQQLRQADPSLYLSPSANLAAAARAALKHLHSSLAVASPVQPPPLQDLLAGPEFDAEQIWSQSELLARPLLPHLHRQLRRLEHQAPPPEPSKPAEAEEEEEVSEDEEGGDGDDGDELNEAELEDIDDELESGEDEDEEEEEDDEEEETEELEGKAGNRVEDKFLKINELEKFLEQGEEAEYGGSSKEGEKKKKKKAGVNWMEDESDEDMDEDGDEDEDDDDLDLEDIEYDDDEEDGGKSGGDIRYEDFFEGKTKQQVKKRNGSTKKVQFKDELHEVKVDHTENDDGNVGPALEDEQGLSTHEKERLKMHAKIEQMEKASLEPSVWTMKGEVTASSRPRNSALEVDLDFEHNVRPAPVITEEVTASLEEMIKKRIAEGHFDDVEKPSTLPSKAPKEQKEMDENKSKKGLAELYEEDYVKEAGLAPAPLSISDELKKEANTLFKRICLKLDALSHFHFAPKPVIEDMSIQANVPALAMEEVAPVAVSDAAMLAPEEVFEGKGDIKEEAELTQAERKRRRANKKRRYAESHKDKPAESHKERPAKLQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1IWH1</accession>
    <name>I1IWH1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DSBA-like thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100834992</name>
        <name evidence="3" type="ORF">BRADI_5g04030v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581462.1">
        <property type="nucleotide sequence ID" value="XM_003581414.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IWH1"/>
    <dbReference type="FunCoup" id="I1IWH1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IWH1"/>
    <dbReference type="EnsemblPlants" id="KQJ81952">
        <property type="protein sequence ID" value="KQJ81952"/>
        <property type="gene ID" value="BRADI_5g04030v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834992"/>
    <dbReference type="Gramene" id="KQJ81952">
        <property type="protein sequence ID" value="KQJ81952"/>
        <property type="gene ID" value="BRADI_5g04030v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834992"/>
    <dbReference type="eggNOG" id="KOG2567">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074689_1_1_1"/>
    <dbReference type="OMA" id="NQERFWN"/>
    <dbReference type="OrthoDB" id="37297at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02972">
        <property type="entry name" value="DsbA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875:SF3">
        <property type="entry name" value="OS04G0227500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875">
        <property type="entry name" value="OS09G0542200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095536" description="DSBA-like thioredoxin domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="DSBA-like thioredoxin" evidence="2">
        <location>
            <begin position="46"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ81952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="219" mass="23836" checksum="C5B7C470BD764426" modified="2012-06-13" version="1">MGPSTLLLVVVAMAAAMGGCFAQMPIPARTDGFAYGKATAWGEAVVVEAYFDPVCPDSRDAWPPLQKAVQHYGAQRVSVVVHLFPLPYHSNAFIACRSIHTVNKLNASAVYPLLEKFFKYQEAYYNQPTYTKSRETVVAEITNNLVAPVIGEANLAAYKAGFNDSMSDQSTRISFKYGCARGVTGTPYFFVNGIPLGDSGSPVDYDKWISTLDPLVGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>I1IYN5</accession>
    <name>I1IYN5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit C</fullName>
            <shortName evidence="6">eIF3c</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF3 p110</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100832515</name>
        <name evidence="9" type="ORF">BRADI_5g12910v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-3 subunit C family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579937.1">
        <property type="nucleotide sequence ID" value="XM_003579889.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IYN5"/>
    <dbReference type="FunCoup" id="I1IYN5">
        <property type="interactions" value="2789"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IYN5"/>
    <dbReference type="EnsemblPlants" id="KQJ83074">
        <property type="protein sequence ID" value="KQJ83074"/>
        <property type="gene ID" value="BRADI_5g12910v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83075">
        <property type="protein sequence ID" value="KQJ83075"/>
        <property type="gene ID" value="BRADI_5g12910v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832515"/>
    <dbReference type="Gramene" id="KQJ83074">
        <property type="protein sequence ID" value="KQJ83074"/>
        <property type="gene ID" value="BRADI_5g12910v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83075">
        <property type="protein sequence ID" value="KQJ83075"/>
        <property type="gene ID" value="BRADI_5g12910v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832515"/>
    <dbReference type="eggNOG" id="KOG1076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004304_0_1_1"/>
    <dbReference type="OMA" id="MNINNWV"/>
    <dbReference type="OrthoDB" id="29647at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IYN5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000461">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03002">
        <property type="entry name" value="eIF3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027516">
        <property type="entry name" value="EIF3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008905">
        <property type="entry name" value="EIF3C_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058999">
        <property type="entry name" value="EIF3CL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITATION FACTOR 3, SUBUNIT 8 EIF3S8 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05470">
        <property type="entry name" value="eIF-3c_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26569">
        <property type="entry name" value="EIF3CL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="629"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="174"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="827"/>
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        </location>
    </feature>
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        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
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        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
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        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
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        <location>
            <begin position="215"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="864"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="899"/>
            <end position="908"/>
        </location>
    </feature>
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        <location>
            <begin position="916"/>
            <end position="930"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00057041"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ83074.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="940" mass="106035" checksum="18AA82A9E8A3EB34" modified="2012-06-13" version="1">MASRFWGQGASDSEEEVEDIDSEQGGDSEKSEAGDGGREGSKNPYLSKYTSDSDDSDVDNPRVIRSLKDKRNDEMKATADQMRNAIKINDWLSLQESFDKLNKQLEKVVRINESTKVPNRYITTLVLLEDFLTQTLANKEAKDKMSRSNSKALNAMKQKLKKNNKQYEDLIQKCRENPESFEDDVADEKDVDDEDEDDDDSGEDIVDPDKMALSESEESGNEDNEGEEGGGWEKKLSKKDKIMDKQFMKDPSEITWDIVDKKLKEIVASRGKKGTGRVERVEQLTFLTRVAKTPAQKLEILFHVISAQFDVNPSLLGHMPVIVWKKCVNNMLIVLDILQQYPNIVVDTSVEPDEKETQKGADYNGTIHVTGDLVAFLERIDSEFFKSLQCSDPYTKDYVQRLRDEPLFSVLAQTVQEYQERVGNLKAAAKVALRRVELVYYKPQEVYDAMRKLAEQTEAGIEDGDADTGDEYQAADNYRGTPPFVVIPEVVPRKPTFPESGRVLMDGLMSLIYKYGDERTKARAMLCHIYHYAISDEFSVARDLLLMSRLQDGVQLLDISSQILFNRAMAQLGLCAFRAGLIAEAHSCLSELYSTGRVKELLAQGVQQSRYHEKTPEQERLERRRQMPYHMHINLELLEAAHLICAMLIEVPNMAANTFDKRRPMSKTFRRLLDMSERQTFVGPPETVRDHVLAATRALNKGDHEKAFSVIASLDTWKLLRNKDHILEMLKLKIKEEALRTYMFSYSSCYESLSLVQLTTMFDLNESQAHSIVSKMMMLEELHASWDQPTKCIIFHSVDQTRLQGLLFQMADRLSVLVESNERAYEARTGGALEGAPPRRRGGDGQDSSNLGKWQENFASSQGRRGGGRFGYSGRTGGSGRGGGGYQNDRFQNDRSGQGSRGGYGGGSRFHDGRTRTQSGSSSRGDGSARMVSLNRAGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IYW3</accession>
    <name>I1IYW3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Surfeit locus protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100844943</name>
        <name evidence="2" type="ORF">BRADI_5g13520v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NM_001419630.1"/>
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    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83172">
        <property type="protein sequence ID" value="KQJ83172"/>
        <property type="gene ID" value="BRADI_5g13520v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844943"/>
    <dbReference type="Gramene" id="KQJ83172">
        <property type="protein sequence ID" value="KQJ83172"/>
        <property type="gene ID" value="BRADI_5g13520v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRC9">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47854:SF1">
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    </dbReference>
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            <end position="153"/>
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        <location>
            <begin position="262"/>
            <end position="271"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ83172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="29786" checksum="E17400F493C572AD" modified="2012-06-13" version="1">MAKKSKAAAAPDSGMSSPQGSVEGGAEKEGSFLLGQPTWEDAGGGRWRCAETGHELPEREKDSYARSRACRIALIDHGVAHKKPPLSAFKPHPEHKSKLICNITGDTINKSEEHIWKHVNGKRFLNKLEKLEEKMTSSETTKGEVEQSKEVAKKSKSSKKKDKKKAAVVDTSLPREPQPEIDDSDDSEFWVPPVGSRWDDDDGKDRWESPPGKDDVAEDEGASDEDGDDDMADEDDDVSNELASSSTKRLSVEAVGPSSFASRKKKPRKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZA9</accession>
    <name>I1IZA9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100822809</name>
        <name evidence="8" type="ORF">BRADI_5g14687v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F. Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28. Two forms of p82 have been identified, p82-34 and p82-16.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4G family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQJ83387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240075.1">
        <property type="nucleotide sequence ID" value="XM_010241773.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZA9"/>
    <dbReference type="FunCoup" id="I1IZA9">
        <property type="interactions" value="2012"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IZA9"/>
    <dbReference type="EnsemblPlants" id="KQJ83387">
        <property type="protein sequence ID" value="KQJ83387"/>
        <property type="gene ID" value="BRADI_5g14687v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822809"/>
    <dbReference type="Gramene" id="KQJ83387">
        <property type="protein sequence ID" value="KQJ83387"/>
        <property type="gene ID" value="BRADI_5g14687v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822809"/>
    <dbReference type="eggNOG" id="KOG0401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021872_0_0_1"/>
    <dbReference type="OMA" id="MEKDHQP"/>
    <dbReference type="OrthoDB" id="514777at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000027">
        <property type="entry name" value="Eukaryotic translation initiation factor isoform 4G-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000036">
        <property type="entry name" value="Eukaryotic translation initiation factor isoform 4G-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253:SF81">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR ISOFORM 4G-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="MI" evidence="7">
        <location>
            <begin position="626"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="113"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="525"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="583"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ83387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="792" mass="87876" checksum="BEA69658A345F0B2" modified="2012-06-13" version="1">MTTDQPVISLRPGGGGGGPRASRLFSPAFATAASGSGDFLRPHGGAASGVSRIGDSHFETRERIRYSRDQLLELREIADVPEDISRIKQEIEAELQGLLQVDDQSWVRNDSIVPAQTQTQTQTQAQAQNRYTETDNRDWRTRTVQPPPVHEEKSWDNIRENKELYNSSGRQQEQFNRQDQLSSQFVSKAQVGPPPALIKAEVPWSARRGNLTEKDRVLKTVKGILNKLTPEKFDLLKGQLLESGITTADILKDVISLIFEKAVFEPTFCQMYAQLCSELNDNLPSFPPEESGGKEITFKRVLLNNCQEAFEGADSLRVEIARLIEPDQEMERRDKERIFKLRTLGNIRLIGELLKQKMVPEKIVHHIVKELLGSDKKACPDEEHVEAICQFFNTIGKQLDENPKSRRINDTYFIQIKELVANPQLTPRSKFMVRDLIDLRSNNWVPRRAEIKAKTISEIHSEAEKNLGLRPGTTMRNGRNAPGGPLSPGGFPMNRPGIGGMMPGMPGSRKMPGMPGLDNDNWEVQRSKSMPRGDPLRNQGQSVNKLSSINKPLPINHKLLPQGSGALMGKSALLGTGGPPSRPSSFTSPIQSPGQATASPKPLSATPAPVLVPDKAATSSKVIPAELQKKTIALLEEYFGIRILDEAQQCIEELQSPAYYPEIVKEAINLALDKGASFVDPLVRLLEHLYTKKIFKTEDLENGCLLYGSLLEDIGIDLPKAPTQFGEIVARLILSCGLRFEAVEEILKKMDDTFFRKEIFKAVTKTLEANSPGQAILSSHAAVIDACNNLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1J188</accession>
    <name>I1J188_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100836920</name>
        <name evidence="4" type="ORF">BRADI_5g20150v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580458.1">
        <property type="nucleotide sequence ID" value="XM_003580410.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J188"/>
    <dbReference type="EnsemblPlants" id="KQJ84329">
        <property type="protein sequence ID" value="KQJ84329"/>
        <property type="gene ID" value="BRADI_5g20150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836920"/>
    <dbReference type="Gramene" id="KQJ84329">
        <property type="protein sequence ID" value="KQJ84329"/>
        <property type="gene ID" value="BRADI_5g20150v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836920"/>
    <dbReference type="eggNOG" id="ENOG502QRKA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060647_0_0_1"/>
    <dbReference type="OMA" id="HLEMQSE"/>
    <dbReference type="OrthoDB" id="7763451at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J188">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343:SF75">
        <property type="entry name" value="OS04G0594400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343">
        <property type="entry name" value="SERINE/ARGININE-RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="7"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="237"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ84329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="314" mass="33140" checksum="49484833DBC0C022" modified="2012-06-13" version="1">MATSTLSTVMVSNLSLKAAQRDVKEFFSFSGDILHVEMQSADELSQVAYITFKDKQGAETAILLTGATIVDMAVIVTPATDYEVPASVLAALEPKDGKSAVLEKAEDIVGTMLAKGFILGRDALDKAKALDEKHQLTSTATARVSSFDKRIGLSEKISVGSSVVNDKVKEMDQKYLVSEKTKSALAAAEQGVSTAGSAIMKNRYVLTGAAWVTGAFSKVANAANDVGAKAKEKIVAEQEGKTVERESAQGNISDDPAKHKDSDDDFAKVHVSETTEDIPISRAATVPITEEGSSNATPLPPAPKKPEPAQGLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1J1F9</accession>
    <name>I1J1F9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="8">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100842523</name>
        <name evidence="10" type="ORF">BRADI_5g20718v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Extracellular space</location>
            <location evidence="8">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="8"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581615.1">
        <property type="nucleotide sequence ID" value="XM_003581567.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J1F9"/>
    <dbReference type="FunCoup" id="I1J1F9">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J1F9"/>
    <dbReference type="EnsemblPlants" id="KQJ84411">
        <property type="protein sequence ID" value="KQJ84411"/>
        <property type="gene ID" value="BRADI_5g20718v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842523"/>
    <dbReference type="Gramene" id="KQJ84411">
        <property type="protein sequence ID" value="KQJ84411"/>
        <property type="gene ID" value="BRADI_5g20718v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842523"/>
    <dbReference type="eggNOG" id="ENOG502QQ71">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048041_0_1_1"/>
    <dbReference type="OMA" id="DAWTRNT"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02176">
        <property type="entry name" value="GH16_XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008263">
        <property type="entry name" value="GH16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01034">
        <property type="entry name" value="GH16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0052">Apoplast</keyword>
    <keyword evidence="8" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="1 8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982900" description="Xyloglucan endotransglucosylase/hydrolase" evidence="8">
        <location>
            <begin position="31"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="9">
        <location>
            <begin position="43"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="7">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ84411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="316" mass="34229" checksum="22733DE5739B78A5" modified="2012-06-13" version="1">MARSSLLSAPAVAVATAAIAIACLATCSSAAAGGGNFYENFVVAWGDDPDPGRRVEVLDGGRVVTLTLNNVSGGSGFQSKDAFLFGEFTMPMKLVPGDSAGTVTTFYLTSKDPTAEGDGHDEIDFEFLGNVSGEPYVMQTNVFARGVGNREQRFYLWFDPTADFHNYTILWNPLNIIFSVDGVAVRVFRNHERHGVPYLSGQAMKVHATIWNDDTWVTMGGRVKTDWSHAPFVAAYGAYSATACVSSPAAETSDEGGGSDCDGAAEEETSSWMARRLGPDGERAVAWAREKYMVMDYCDDPWHLGRPAECDIDRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E771</accession>
    <name>A0A0Q3E771_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g06504v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E771"/>
    <dbReference type="EnsemblPlants" id="KQJ82132">
        <property type="protein sequence ID" value="KQJ82132"/>
        <property type="gene ID" value="BRADI_5g06504v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82132">
        <property type="protein sequence ID" value="KQJ82132"/>
        <property type="gene ID" value="BRADI_5g06504v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E771"/>
    <dbReference type="OrthoDB" id="693867at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033238914" evidence="1">
        <location>
            <begin position="23"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ82132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="65" mass="6811" checksum="3E41962BBF3FBDE1" modified="2016-01-20" version="1">MAATAMMAKLVVVLLFLTQIFGLHLAAAARPLQEGHGAWMENGIEMLTQLLGGSKSGSNGRGHCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ECX7</accession>
    <name>A0A0Q3ECX7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100846866</name>
        <name evidence="9" type="ORF">BRADI_4g00486v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; phylloquinone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 7/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family. DHNA-CoA hydrolase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579079.1">
        <property type="nucleotide sequence ID" value="XM_003579031.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ECX7"/>
    <dbReference type="STRING" id="15368.A0A0Q3ECX7"/>
    <dbReference type="EnsemblPlants" id="KQJ85598">
        <property type="protein sequence ID" value="KQJ85598"/>
        <property type="gene ID" value="BRADI_4g00486v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846866"/>
    <dbReference type="Gramene" id="KQJ85598">
        <property type="protein sequence ID" value="KQJ85598"/>
        <property type="gene ID" value="BRADI_4g00486v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846866"/>
    <dbReference type="OrthoDB" id="46529at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061522">
        <property type="term" value="F:1,4-dihydroxy-2-naphthoyl-CoA thioesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042372">
        <property type="term" value="P:phylloquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000048">
        <property type="entry name" value="14-dihydroxy-2-naphthoyl-CoA thioesterase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43240">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOYL-COA THIOESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43240:SF15">
        <property type="entry name" value="THIOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="8">
        <location>
            <begin position="51"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ85598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="177" mass="18715" checksum="157230F5EE54BA47" modified="2016-01-20" version="1">MADETAAATAKPKTAELDPVLHAMGFELESLSPSLLTGRLPVTSRCCQPFKVLHGGVSALVSEAMASMGAHMASGYSRVAGVHLAINHFRSAAVGDVVVARAVPVHLGRSTQVWEVKLWKLLVEPPEPEKKKVLIAESRVTLLCNLPVPESLRHAGDALKKYAAAADANPVAPISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EDP7</accession>
    <name>A0A0Q3EDP7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g21612v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84572.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EDP7"/>
    <dbReference type="EnsemblPlants" id="KQJ84572">
        <property type="protein sequence ID" value="KQJ84572"/>
        <property type="gene ID" value="BRADI_5g21612v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ84572">
        <property type="protein sequence ID" value="KQJ84572"/>
        <property type="gene ID" value="BRADI_5g21612v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EDP7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ84572.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="123" mass="12813" checksum="567BE5723023AA2C" modified="2018-03-28" version="2">MKETTTPRCRPPHPSRWTRTARLGRSSRSSSSMAQLKKKKPPPLPSHPTPPRPLLDASAAAPRSSASPPAASSSPQPQPNVVGSAAPPLNTAAGSLATGVDALLWPEAPRDLFQAAPSPSSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EFI9</accession>
    <name>A0A0Q3EFI9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g05490v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EFI9"/>
    <dbReference type="FunCoup" id="A0A0Q3EFI9">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ86439">
        <property type="protein sequence ID" value="KQJ86439"/>
        <property type="gene ID" value="BRADI_4g05490v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ86439">
        <property type="protein sequence ID" value="KQJ86439"/>
        <property type="gene ID" value="BRADI_4g05490v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EFI9"/>
    <dbReference type="OrthoDB" id="597540at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EFI9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111">
        <property type="entry name" value="BNAA05G37150D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111:SF136">
        <property type="entry name" value="F-BOX ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="12"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ86439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="707" mass="79553" checksum="0E48CD407A8B182A" modified="2016-01-20" version="1">MELSEATTAEENQAVAPLPKEILMEILARLPAKSVGRFRCVSPDWSAMLSSAYFVDLHARRANRPDRPRLLLAPVGSSYDDCVYSWQPGGQVEKLRSDDFAETGLLAPVTKPCHGLVLIRCTDYRGYFVCNPSTGEVLPLPDSEVPLKTIWRPSISGQPFFYGVSYGLGYCSVTKQHKVVRLFWCNNVSSCEVFVLDKLAYWRPTAQEPPSCHVSEDKLAVFVRGHLHFLCRGADIITFNTSSETFGSLLPPAGFEDASPLLTELDDCLCYCYGEPDSDDPYHVFLLRDYMGGRWEKLCCIERSAWPESERMLLRSLCISPLVMYHSDDGQRRVMFGTGACKVFAVGLDTNIPEILFTPDGTIIGSCDDDYILPLCLFEEYLGSVGRTVEEMAFSSPTTKAWSDIFKWMPARSVSELSLIPRVMIVVDLQFGQYMNLKDFFDAGAPVLHRNLVCTPQPCHGLNAWSLGSWDFICNPVMGYREHIESDNDNGTSFAGRIGLGYHSAINKHVVVRVTYKEKNLDTRDYELQCELRYVKEKQWRSIDPPPRPIADMPPTYPNLGPIFLRCEIVAFSVETEEFEVLQGPPCSHDNGRISILQLQGALCIACSDKSKNVLDIWMMMDIDTWLMRYHIELEEFLPHYLSENTTPLVVDPKDGRILLNTGLSLGYYDPKMAALETVYSVGIVKHDCTFCPIICDESLVCPFGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2024-10-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EJI5</accession>
    <name>A0A0Q3EJI5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g13931v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EJI5"/>
    <dbReference type="EnsemblPlants" id="KQJ87854">
        <property type="protein sequence ID" value="KQJ87854"/>
        <property type="gene ID" value="BRADI_4g13931v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87854">
        <property type="protein sequence ID" value="KQJ87854"/>
        <property type="gene ID" value="BRADI_4g13931v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EJI5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ87854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="152" mass="16277" checksum="78A9FE87355621AE" modified="2016-01-20" version="1">MNAYESTQECILRFCPPLHIPIQARAPLMCALTVSFFFVRNSGREQAVLVLVSPVASFFGLLCRGTMSDIDAGCGDDDLSSDSNSEFSDFVPANWHGAANAGGPAAANAVSDSHLICASFFICWPFHLICAPFIGTSGKDSKITKIKPTKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ESM0</accession>
    <name>A0A0Q3ESM0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100841579</name>
        <name evidence="10" type="ORF">BRADI_4g31937v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578215.1">
        <property type="nucleotide sequence ID" value="XM_003578167.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ESM0"/>
    <dbReference type="FunCoup" id="A0A0Q3ESM0">
        <property type="interactions" value="1030"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3ESM0"/>
    <dbReference type="EnsemblPlants" id="KQJ90494">
        <property type="protein sequence ID" value="KQJ90494"/>
        <property type="gene ID" value="BRADI_4g31937v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT64711">
        <property type="protein sequence ID" value="PNT64711"/>
        <property type="gene ID" value="BRADI_4g31937v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841579"/>
    <dbReference type="Gramene" id="KQJ90494">
        <property type="protein sequence ID" value="KQJ90494"/>
        <property type="gene ID" value="BRADI_4g31937v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64711">
        <property type="protein sequence ID" value="PNT64711"/>
        <property type="gene ID" value="BRADI_4g31937v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841579"/>
    <dbReference type="OrthoDB" id="2538017at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3ESM0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024882">
        <property type="entry name" value="NUP58/p45/49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13437">
        <property type="entry name" value="NUCLEOPORIN P58/P45 NUCLEOPORIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13437:SF2">
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
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            <strain evidence="1 2">Bd21</strain>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Wang M."/>
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                <person name="Vogel J.P."/>
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        <source>
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    </evidence>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
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            <strain evidence="1 2">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
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                <person name="Mockler T.C."/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00680"/>
        </source>
    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
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    <name>A0A0Q3FTF0_BRADI</name>
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            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_014898332.2"/>
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    <dbReference type="STRING" id="15368.A0A0Q3FTF0"/>
    <dbReference type="EnsemblPlants" id="KQK02680">
        <property type="protein sequence ID" value="KQK02680"/>
        <property type="gene ID" value="BRADI_2g03040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827272"/>
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        <property type="gene ID" value="BRADI_2g03040v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3027644at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FTF0">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF70">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="241"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK02680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="475" mass="53472" checksum="C5F6A9D17B882730" modified="2016-01-20" version="1">MVRANAHRAALPLLCSHQWLISLITCPYSAFSFLPLWISGSEFVWSMATILDSLLRSCANKLQDIITNEAILILRVEKELAEVLRRVELIRCCIADAEKRRTKDLAVNNWLGQLSDIIYDVDELLDVAICKGSKLLPDHTSSSSSKSAACKGPSVFSCFCNIGPRRNVAVRIRSLNKKIENISKDKIFLTFNRSSTQPTGNGSTSKLIRSSNLVEPNLVGKEIRHSSRKLVDLVLAHKESKSYKIAIVGTGGVGKTTLAQKIYNDQKIKGSFNMRAWVCVSQDCNEVTLLKEILRNINVHHEEGETIGELQRKLAETIEGKSFFLVLDDVWQSNVWTDLLRTPLHSTSAGLVLATTRDDKIAMIIGVEDIHRVDLMSVEVGWELLWKSMNIDDEKEVQHLRNIGNEIVRKCGRLPLAIKARTNGAIHRFEGQIVRRSKLRKLVRVEDVQVRPLCVAIELVKEVSCFIFILDRLMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FYC2</accession>
    <name>A0A0Q3FYC2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_2g07116v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FYC2"/>
    <dbReference type="STRING" id="15368.A0A0Q3FYC2"/>
    <dbReference type="EnsemblPlants" id="KQK03325">
        <property type="protein sequence ID" value="KQK03325"/>
        <property type="gene ID" value="BRADI_2g07116v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03325">
        <property type="protein sequence ID" value="KQK03325"/>
        <property type="gene ID" value="BRADI_2g07116v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FYC2"/>
    <dbReference type="OrthoDB" id="73614at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF29">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK03325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="101" mass="11090" checksum="C000937B14A78966" modified="2016-01-20" version="1">MDALARLHRSLAGGDDEEQPGEDILGDTEGLCSLSPLQRVYGFAACLVAGLALMMLVHDKLLTLIAIICEICALFWYSLSYIPFARRVVSDLMVKLCDTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3G8E2</accession>
    <name>A0A0Q3G8E2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g28296v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3G8E2"/>
    <dbReference type="EnsemblPlants" id="KQK06757">
        <property type="protein sequence ID" value="KQK06757"/>
        <property type="gene ID" value="BRADI_2g28296v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06757">
        <property type="protein sequence ID" value="KQK06757"/>
        <property type="gene ID" value="BRADI_2g28296v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3G8E2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK06757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="119" mass="13170" checksum="B9008E32159B5190" modified="2016-01-20" version="1">MNQSLLPFGFCSCFMWRILRIARRGSSCELRTEPTEAELVLLIVSEVADLQRRRAAWYGVGWRLVSHSGFRAIGKPFAAPRSNGKTRSIQLWGGTANRAASTIVPSRSVASSSNLQLCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GKR8</accession>
    <name>A0A0Q3GKR8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g00438v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81394.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GKR8"/>
    <dbReference type="FunCoup" id="A0A0Q3GKR8">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ81394">
        <property type="protein sequence ID" value="KQJ81394"/>
        <property type="gene ID" value="BRADI_5g00438v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81394">
        <property type="protein sequence ID" value="KQJ81394"/>
        <property type="gene ID" value="BRADI_5g00438v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GKR8"/>
    <dbReference type="OrthoDB" id="695277at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835:SF68">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ81394.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="221" mass="24678" checksum="9451E50C6EDDFE1C" modified="2018-03-28" version="2">MSLSKLKAKAAHSDTTAPLSTDDAMQYDSNDESQTSSTSKSATSRTSAKTLYWIIKKVNPIKRQLVKEIGFGGLLELPLWNSISRIFSTWLLSQVDCIDLAIILDATRRLLFVPQDVNRVFGMPCRHRDVLGPETKISDAAMAYIREQAGITMTKISLIDAEKIVLMDLSEDSTRLQKDSFKMAFVIIAMGHLLSPSTKYDHVNIDFLGALRCTEEIGQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GPR5</accession>
    <name>A0A0Q3GPR5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g03555v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GPR5"/>
    <dbReference type="EnsemblPlants" id="KQK12405">
        <property type="protein sequence ID" value="KQK12405"/>
        <property type="gene ID" value="BRADI_1g03555v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12405">
        <property type="protein sequence ID" value="KQK12405"/>
        <property type="gene ID" value="BRADI_1g03555v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GPR5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK12405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="200" mass="22111" checksum="26898C966A076F49" modified="2016-01-20" version="1">MPCLDLPPAFHLLHCPCPRCLERVLGRIKGRALHHLLLHAASDGHPCCAAVHHLTTAPCFLAAWRRPRASCASPKPLASHRCCSLHTRPHLVLCLPAPPLQPAHAAKTHTGRTRLTHRKSWGSPQEPPPPDPLPPVALIPCGTRPHLGQRAYGFRGLVWRLQFLGRTGERAGRKTLRSGYLMGLGPAWANYIQQKLQYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GV46</accession>
    <name>A0A0Q3GV46_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g14915v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GV46"/>
    <dbReference type="FunCoup" id="A0A0Q3GV46">
        <property type="interactions" value="1521"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GV46"/>
    <dbReference type="EnsemblPlants" id="KQK14245">
        <property type="protein sequence ID" value="KQK14245"/>
        <property type="gene ID" value="BRADI_1g14915v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK14245">
        <property type="protein sequence ID" value="KQK14245"/>
        <property type="gene ID" value="BRADI_1g14915v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GV46"/>
    <dbReference type="OrthoDB" id="678486at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33286">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33286:SF52">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033724763" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK14245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="97" mass="11177" checksum="3A2460E94DBE4EDD" modified="2016-01-20" version="1">MAKLMCLLLVVLAIAVTTWAESCDKDREDMISECYKYEKWPGEPSEACCAVWKRANIPCIRKLITKEIEKMCCMKNVRYIAKFCKKPFPPGYKCGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GZ77</accession>
    <name>A0A0Q3GZ77_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THO complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100846277</name>
        <name evidence="10" type="ORF">BRADI_1g24950v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A0Q3GZ77">
        <property type="interactions" value="2645"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GZ77"/>
    <dbReference type="EnsemblPlants" id="KQK15797">
        <property type="protein sequence ID" value="KQK15797"/>
        <property type="gene ID" value="BRADI_1g24950v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15797">
        <property type="protein sequence ID" value="KQK15797"/>
        <property type="gene ID" value="BRADI_1g24950v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="29024at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GZ77">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040007">
        <property type="entry name" value="Tho2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021418">
        <property type="entry name" value="THO_THOC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021726">
        <property type="entry name" value="THO_THOC2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032302">
        <property type="entry name" value="THOC2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597">
        <property type="entry name" value="THO2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11262">
        <property type="entry name" value="Tho2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11732">
        <property type="entry name" value="Thoc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16134">
        <property type="entry name" value="THOC2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="9">
        <location>
            <begin position="38"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="9">
        <location>
            <begin position="443"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 N-terminal" evidence="8">
        <location>
            <begin position="576"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 C-terminal" evidence="7">
        <location>
            <begin position="894"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1207"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1383"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="917"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1220"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1231"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1264"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1284"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1323"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1335"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1427"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1486"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1617"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1636"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1746"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1768"/>
            <end position="1777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK15797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1777" mass="202146" checksum="9F1F166D47B668DC" modified="2016-01-20" version="1">MSPPLQAPDYKYVTEECLREWKSQSAAGFRLPDPVPMARFLYELCWAIVRGDLPPQKCRVALDSVVFVEEAWHEESGSVLADIVAHLGQDITISGEYRNRLVKMTKSFVESSLIVPRLLQERCEEEFLWEVEQSKSKGQDLKAKEVRVNTRLLYQQTKFNLLREESEGYAKLVTLLCQVGSDLACQNASSATISIIKSLIGHFDLDPNRVFDIVLECFELYPDNSTFYQLIPLFPKSHAAQILGFKFQYYQQLDVNSPVPSGLFRIAALLVKSGLIDLDNLYAHLLPNDDEAFEHFGSFVSRRIDEATKIGKINLAATGKDLMDDEKQEITIDLYTALEMENDIIEERAPEIEKNQKLGLLLGFLSVHDWDHAQLLFERLAQLNPVEHIEICDGLFRVIEKTISSAYTIYCQAHHKIPGNIDTHMVEASSVSSPSYLVDLPKEFFQMLAACGPYLHRDTQLFQKVCRVLKVYHVRSKDSARTAGVMSSESRVEEALGSCLLPSLQLIPANPAVDMEIWGVLSLLPYEVRYRLYGEWEKDTEQNPIVLAARQTAKLDTRRLLKRLAKENLKQLGRMVAKLAHANPMTVLRTIVTQVEAYRDMINPVVDAFKYLTQLEYDILQYIVIERLAQGGREKVKDDGLNLSDWLQCLASFWGHLCKKHLSMELKCLFQYIVNQLKKGLGTELVVLEELIQQMANVQYTENMTDEQVDAMAGSETLRLQSSLFGSTRNYKVLNKSMNKLRDSLLPKDEPTLAIPLLLLIAQHRSKIIINADATYIKMVSEQFDRCHGILLQYAEFLSSAVTPAIYIQLIPPLEDLVYKYHIEPDVAFLIYRPVMRLFKSANGGDTCWPLDDNEEGESVSCDDMILHGDSSQKLIMWSDLLNTIRTILPTKAWIALSPELYATFWGLTLYDLHFPKDRYDAEIKKLHDNLKQLEDNSDNSSIAISRRKKDKERIQDLLDKLNNESEKHQQHVVSVFQRLTREKDKWLSSSPDALKINMEFLQRCVYPRCVLSMQDAVYCATFVQTMHSLGTPFFNTVNHIDVFICKTLQPMICCCTEYEAGRLGRFLHETLKMAYHWKSDESVYERECGNKPGFAVYFRFPNSQRVSYTQFIKVHWKWSGRITKVLNQCMESKEYMEIRNALIVLTKITSIFPVIRKSGVNIEKRVAKLKGDEREDLKVLATGVAAALAARKSSWLSEEEFGMGHLDTKPVPAKPIPGNHSSDPSTAKDQIVRAKSTEGRHERTENAMKPDAQHKNNPLPANGSDSQIPSSVAQGKVSGVARGSDEPPKLLSDEGVKVLKPTTESETRVSQKRATHNAAKVSKHDAVKEHAKSGKSTSRGLNQQASSLPIDRQVLSQAADGVLDTNPTSSFVGTNGNVHLVPRKVSASSQRSTVLAAHSGGTANPTGEGESTDLIDSTVKQQKRSVPAEEQERISKRRKGDIQGRDGELTEQHTDKERKLDTRSADRFRSADHERGASEEQNIIRTDKLKEKFDDKHDRDPREKADQLERRRGEDVIERPLDRASDRRERSIDRMQDMAPEKGREDRNKEDRNKAKHAEPSVDRAHSSDERFPGQSLPPPPPLPASFVPQSVGSNRRDEDSGRRGGSTRHTQRLSPRRDEKERWHLEENASLLQDDGKHRREEDLRDRKREDRDVSSSKVDDRDRDKVSTMKEDSDPNSASKRRKIKREQSALEAGEYAPSAPQPPSLGAGGLQLEIRERERKGVISQHRPSHADDLPRITSKTSRREVDQTLEREWEEEKRPRTEAKRKHWKSYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H531</accession>
    <name>A0A0Q3H531_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g19595v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88562.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H531"/>
    <dbReference type="EnsemblPlants" id="KQJ88562">
        <property type="protein sequence ID" value="KQJ88562"/>
        <property type="gene ID" value="BRADI_4g19595v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88562">
        <property type="protein sequence ID" value="KQJ88562"/>
        <property type="gene ID" value="BRADI_4g19595v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H531"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ88562.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="68" mass="7006" checksum="29641CEB49AD1228" modified="2018-03-28" version="2">MNQSSLPPTSSQVTTAPASTGKSCSNRRSGLAELRTGQSGGQDGRKQGMGQGYRASGISSLIFMKNQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HWQ9</accession>
    <name>A0A0Q3HWQ9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDSL esterase/lipase EXL3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100844377</name>
        <name evidence="3" type="ORF">BRADI_3g00311v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570760.4">
        <property type="nucleotide sequence ID" value="XM_003570712.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HWQ9"/>
    <dbReference type="EnsemblPlants" id="KQJ92731">
        <property type="protein sequence ID" value="KQJ92731"/>
        <property type="gene ID" value="BRADI_3g00311v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844377"/>
    <dbReference type="Gramene" id="KQJ92731">
        <property type="protein sequence ID" value="KQJ92731"/>
        <property type="gene ID" value="BRADI_3g00311v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HWQ9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF110">
        <property type="entry name" value="OS02G0101400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="48619" checksum="E062D26FF837A4AD" modified="2016-01-20" version="1">MDSRQARTRTSLASINSPHVTHLLSCRPVTVISAPKAMHKYIPQHNGHRHNTSSLHCTRSSVRIDHSSQSDRSMGVTRCLQRSSRSLFLLMLLVAAAISSVSSRDAAIAASSSSASPKKKKPKVPALVVFGDSIVDPGNNNDINTIVKANFRPYGKDFGRDHRPTGRFCNGRIPTDFIASRLGLKELLPAYLTPNLTNQDILTGVSFASGGTGYDPLTAQLATVISMTDQLRMFEDYKQKVRAAGGDAALATMLSDGVFAVCAGSDDVANTYFTMRARSDYDHASYAALMVDHATSFLDGLLAAGARRVAVISVPPIGCVPSQRTLSGGMARDCSQGHNEVATMVNAGMTKSMDTLKAKHPGAKLVLMDIYGFLLDMMMRPQSYGFKESTLGCCGTGMMEVSVLCNGVTSAVCGEVKDYLFWDSYHPTEKAYKILVDFVYDNYLNQIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HZV0</accession>
    <name>A0A0Q3HZV0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC104583951</name>
        <name evidence="9" type="ORF">BRADI_3g06130v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HZV0"/>
    <dbReference type="FunCoup" id="A0A0Q3HZV0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3HZV0"/>
    <dbReference type="EnsemblPlants" id="KQJ93683">
        <property type="protein sequence ID" value="KQJ93683"/>
        <property type="gene ID" value="BRADI_3g06130v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93683">
        <property type="protein sequence ID" value="KQJ93683"/>
        <property type="gene ID" value="BRADI_3g06130v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1933825at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009788">
        <property type="term" value="P:negative regulation of abscisic acid-activated signaling pathway"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZFP3-like"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47287">
        <property type="entry name" value="C2H2 AND C2HC ZINC FINGERS SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47287:SF15">
        <property type="entry name" value="ZINC FINGER PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
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        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ93683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="161" mass="17585" checksum="9679ECCF55CBBD16" modified="2016-01-20" version="1" fragment="single">QDMGEPAEGGGASAGSPAHDRRGMIFNDGGAKASLRPVELDLIGTVGAQAPEPEPEPRQLYGCSYCGREFYSSQALGGHQNAHKRERARGRRQLVVAIRGGAFVDADPRWEERAPDFLRMMAAVELVYCTGDERRRRGVGRSGDDRRDSSGEQELDLTLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3INX6</accession>
    <name>A0A0Q3INX6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100831642</name>
        <name evidence="6" type="ORF">BRADI_2g36210v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568953.2">
        <property type="nucleotide sequence ID" value="XM_003568905.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3INX6"/>
    <dbReference type="STRING" id="15368.A0A0Q3INX6"/>
    <dbReference type="EnsemblPlants" id="KQK07555">
        <property type="protein sequence ID" value="KQK07555"/>
        <property type="gene ID" value="BRADI_2g36210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831642"/>
    <dbReference type="Gramene" id="KQK07555">
        <property type="protein sequence ID" value="KQK07555"/>
        <property type="gene ID" value="BRADI_2g36210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831642"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3INX6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17744">
        <property type="entry name" value="BRCT_MDC1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18432">
        <property type="entry name" value="BRCT_PAXIP1_rpt6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051579">
        <property type="entry name" value="DDR_Transcriptional_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23196">
        <property type="entry name" value="PAX TRANSCRIPTION ACTIVATION DOMAIN INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23196:SF1">
        <property type="entry name" value="PAX-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="1021"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="170"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="497"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="609"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="883"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="966"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="213"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="270"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="503"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="523"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="550"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="637"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="883"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="893"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="903"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="916"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="945"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK07555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1215" mass="132546" checksum="79E68902433F6559" modified="2016-01-20" version="1">MVGGEGEETQVVDDEGTPPLGSPVSDSDATRSGGEEDANGDTARSGGGEDDNGDTTWSEGEEDDNGVLYGETQALDDDETQAVDDELEEEEEEEVAGDWAETQVVEDSEDDIDAAEQMVTQAVEECGEDGDIVGEKVKTQVVAKCEEEEDGDGMRGDAGDWARTQLVEECEVEDGGNGDWVDTQLVDECQEEERVSDSGDEDVVDWGRTQLVEDSDEEIGDDGDDELSEGTQEASDDDGLLNDETDVKAHGDTKNSGDSDASTNDEGDTAQDTRQHSTSGTNLLTKIVDNSTSCSASLGDHPGLGIDDESYGYVQSHDKDGSKRKGRCSTAKKLFADATPKNNESKTSCFAGLSYLGSQEPGDLSQANALEVVEGLISINGGVSSQEPTPKKLEKAKPPVSLKMGTLKLAEKVDRCRSSNGKPEIFAWVDSLEDDGGGDFFCKNKDILLHKPAARGKSKYPRARKCSTKIAPAENKITNSKLCGRIETLPSSDSRLLKSDVKKSKRASGNRGKKNLLKDLEDQSNAKPMEGQQQKVSVADSRLLRSDVKKSKRASGNRSKKNLLKDLDDQSNAKPMEGHQQKVSVALNDVGQDTQMAVEAIEALAQSSPAKLSVEGEPPVNRDTGVGSKMAKIHSKNGPQKRSSSIQEGVMTRSKRMKVTTDLNPRPQKGRSRGTKMQENAEDHGTKTKHKPEKSVPQKNKVPNIVDGSKYGGTPIAHRTRHTRNNISEYSELCSNKHLKRSMNLTADNSRVGEVRNSHIAYEPEQPTVSERTTEYGLNSVEKESTEHTCANNAQNLQKCRAPSSHPKQRRTPTAMVQAIAPAVTQTTTDHEIQPEVARPSKKRRVFVTSSELLKYARREPSNGRSTSVLSSIIAKSSASSPILSSSVRVTSKTSGFSSSDQPQKEPSDVEDASRSPKNNFPVQSSALKTPSKVLNKLSPTFSPLNPSKASSRSLSKPSVARELLELDPKNALPNRQRKDSRRKDMSSCSILFSHHLNEDVIKRQKKILARLGVCEAFAISDATHFVADSFFRTMNMLEAITLGKLVVTSMWLESCGQAGSFVDDKKYILRDAKKEREIGFSMPISLASACKHPLLLGKRVFVTPNVKPSRQVITSLVSASSGQPLERIGRSIRKEKEAPDDLLVISCEEDYESCVPLLEKGGKVFDSELLLNGIVIQKLEYERHRLFSDCVKQTRSTRWLKHAVQGRFVPVSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J5V0</accession>
    <name>A0A0Q3J5V0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g39081v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J5V0"/>
    <dbReference type="EnsemblPlants" id="KQK08028">
        <property type="protein sequence ID" value="KQK08028"/>
        <property type="gene ID" value="BRADI_2g39081v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK08029">
        <property type="protein sequence ID" value="KQK08029"/>
        <property type="gene ID" value="BRADI_2g39081v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08028">
        <property type="protein sequence ID" value="KQK08028"/>
        <property type="gene ID" value="BRADI_2g39081v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08029">
        <property type="protein sequence ID" value="KQK08029"/>
        <property type="gene ID" value="BRADI_2g39081v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3J5V0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK08028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="141" mass="15930" checksum="1D7E49F4359FB42A" modified="2016-01-20" version="1">MDTHIAASEFAHNIHILKPKDSSLPPRSTTARGFFLFCFSAGISGSRRQWMLQDGPPAVRRPSAPPAGFVLLLLDLEAAVRTEMYSVSMKRRGTNPHHVDCKAVVHFLLFTLHSSIFLRMHAKEIFEATHGWNPLRQRLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JLB4</accession>
    <name>A0A0Q3JLB4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TFIIB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC104583744</name>
        <name evidence="6" type="ORF">BRADI_3g41330v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT68498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235340.1">
        <property type="nucleotide sequence ID" value="XM_010237038.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JLB4"/>
    <dbReference type="STRING" id="15368.A0A0Q3JLB4"/>
    <dbReference type="EnsemblPlants" id="KQJ99136">
        <property type="protein sequence ID" value="KQJ99136"/>
        <property type="gene ID" value="BRADI_3g41330v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT68498">
        <property type="protein sequence ID" value="PNT68498"/>
        <property type="gene ID" value="BRADI_3g41330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583744"/>
    <dbReference type="Gramene" id="KQJ99136">
        <property type="protein sequence ID" value="KQJ99136"/>
        <property type="gene ID" value="BRADI_3g41330v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68498">
        <property type="protein sequence ID" value="PNT68498"/>
        <property type="gene ID" value="BRADI_3g41330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583744"/>
    <dbReference type="OrthoDB" id="511529at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JLB4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000145">
        <property type="entry name" value="Plant-specific TFIIB-related protein PTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053340">
        <property type="entry name" value="PTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48428">
        <property type="entry name" value="PLANT-SPECIFIC TFIIB-RELATED PROTEIN PTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48428:SF1">
        <property type="entry name" value="PLANT-SPECIFIC TFIIB-RELATED PROTEIN PTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="5">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="491"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ99136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="587" mass="63682" checksum="381B5D1C5743D917" modified="2016-01-20" version="1">MESTTCWSCGEGEVVPDPDSGALVCTSCGRVHDAGASEFVHQATFTAEGKLDLRTSSYVHHSSHAHYLDQKLAGASAVITSTAARLGLSPTRAEEALAMAKSATGGSLATPGTAFLPALAAACAFLVARSHRLPLSLVEAAEAAGCGAAALHDLASRIASTLSLPPLPSFDYSAALERAVRYSHPLSAVQGEKKEAILSQARFLLRCASKWSLTTGRHPLPLVAGLTALAAEVNGVTGVSVEDIAQDISAVMHTSRRRYKELVDALVHVARKLLPWGADVNAKNLLLNAPVLLRLMEMRSQSDPSEQFLESFAPDMSGIVQTYSSVDNDESKYLQIVPLDDLDFNNFGQQGKDSEGSKISEECLSDMYQNVLKKLSEVKELGKFGKGANKRKRWGRGLELDPWMDSQDDGWIKDAPLEEAADIDIGYDAPPPAFTANIELQKRRRGRIEAAKCRIDAIRKAPAVRLAYANGSPAVLINEDVCPPQKIIRKKKGQKRIDGRNHEIQGNRPAETSNALNCQKRQKTGPSDGIDWEDCVIELLLLHGANEAEIEQGQYRRLLELHVFSSRNFVPVRSLKVSQADHGKNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K0Z6</accession>
    <name>A0A0Q3K0Z6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100821300</name>
        <name evidence="14" type="ORF">BRADI_2g13400v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315621.1">
        <property type="nucleotide sequence ID" value="XM_024459853.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315622.1">
        <property type="nucleotide sequence ID" value="XM_024459854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K0Z6"/>
    <dbReference type="FunCoup" id="A0A0Q3K0Z6">
        <property type="interactions" value="1661"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3K0Z6"/>
    <dbReference type="EnsemblPlants" id="KQK04417">
        <property type="protein sequence ID" value="KQK04417"/>
        <property type="gene ID" value="BRADI_2g13400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821300"/>
    <dbReference type="Gramene" id="KQK04417">
        <property type="protein sequence ID" value="KQK04417"/>
        <property type="gene ID" value="BRADI_2g13400v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K0Z6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034051">
        <property type="term" value="P:negative regulation of plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010193">
        <property type="term" value="P:response to ozone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000056">
        <property type="entry name" value="Dual specificity phosphatase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF511">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="46"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="108"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK04417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="198" mass="21850" checksum="23195823F1E59AB3" modified="2016-01-20" version="1">MSVPEAGQSSGRDEREREDYEKQQSRVLMALMQGFCAARYRKADNTPCLVDQGLYLGSVGAALNKEALKSLNITHILIVARSLNPAFSEEFTYKKIEVLDSPDTDLVKHFGECFNFIDEGISTGGNVLVHCFAGRSRSVTVVLAYLMKKHQVSLESALSLVRSKRPQASPNEGFMAQLVNFEKSLQVGNCTMDPNLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K571</accession>
    <name>A0A0Q3K571_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">V-type proton ATPase subunit e1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100824828</name>
        <name evidence="11" type="ORF">BRADI_1g49698v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase e1/e2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557115.1">
        <property type="nucleotide sequence ID" value="XM_003557067.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K571"/>
    <dbReference type="STRING" id="15368.A0A0Q3K571"/>
    <dbReference type="EnsemblPlants" id="KQK19668">
        <property type="protein sequence ID" value="KQK19668"/>
        <property type="gene ID" value="BRADI_1g49698v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824828"/>
    <dbReference type="Gramene" id="KQK19668">
        <property type="protein sequence ID" value="KQK19668"/>
        <property type="gene ID" value="BRADI_1g49698v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824828"/>
    <dbReference type="OrthoDB" id="1508846at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008389">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05493">
        <property type="entry name" value="ATP_synt_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044546035" description="V-type proton ATPase subunit e1" evidence="10">
        <location>
            <begin position="22"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK19668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7598" checksum="90A31B6F909888F1" modified="2016-01-20" version="1">MGFLITTIIFFLAGFVASCLALICCNRGPSTNLFHVTLVITATICCWMMWAIVYLAQMKPLINPILSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3MQV9</accession>
    <name>A0A0Q3MQV9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100831845</name>
        <name evidence="2" type="ORF">BRADI_2g27790v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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            <end position="480"/>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
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        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_3g21017v3"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ96105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96105"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="70" mass="7708" checksum="2ECFDD4A025B4097" modified="2016-01-20" version="1">MRLKATPTQVLSLAGNGVVIPAAPLFLPPLRTEVEATTWASSLKENTQENMVAGGSHQDCTMKEMHRCWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3Q5Y9</accession>
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        </recommendedName>
    </protein>
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        <lineage>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DExH box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3Q5Y9"/>
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        <property type="interactions" value="2378"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3Q5Y9"/>
    <dbReference type="EnsemblPlants" id="KQJ97041">
        <property type="protein sequence ID" value="KQJ97041"/>
        <property type="gene ID" value="BRADI_3g28477v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97041">
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        <property type="gene ID" value="BRADI_3g28477v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="5600252at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent RNA helicase DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000931">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF237">
        <property type="entry name" value="ATP-DEPENDENT DNA_RNA HELICASE DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="274"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="526"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="42"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="196"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ97041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1032" mass="115115" checksum="053180021F952364" modified="2016-01-20" version="1">MLLRSPVCRGSLLFRRSFPFATTTTTRTLRALTTTVAATPVMSYRGGRGGGGTNSQRGRGRGRGGGGRGGRGGGGGGRGEQRWWDPQWRAERLRQIASEVEKVDENEWWNKIGQLREGSQQELVVKKNFGRDGQNILADMAQRHGLYFNAYNKGKTLVFSKVPLPDYRAELDERHGSTQNEIKMSNETERRVETLLSRAQSSNNGSASTSTVLRRSLPSSSSSVVPESTTDVDKQKLSSQLRDLQSSKKMMPSARAMQSFREKLPAFKMREEFLKAVASNQVLVISGETGCGKTTQLPQFILEEEIDNLRGADCSIICTQPRRISAISVAARVGSERGEELGETVGYQIRLESKRSTQTRLLFCTTGVLLRKLVQDPDLVGVSHLLVDEIHERGMNEDFLIIILRDLLPRRPDLRLVLMSATINAELFSKYFGEAPIMHIPGFTFPIAELFLEDILEKTRYKIKSERDNFQGNSRRKRFASVKNDPISDVFEDVDINKEYGNYSSTTRQSLEAWSATDLDLSLVEGTIEYICRHDGEGAILVFLTGWDEISKLVDKIKGNNLLGNSNKFLVLPLHGSMPTVNQREIFDKAPTNMRKIVLATNIAESSITIDDVVYVIDCGKAKETSYDALNKLACLMPSWISKASAHQRRGRAGRVQPGVCYRLYPKVIHDAMPQFQLPEILRTPLQELCLTIKSLQLGAVASFLAKSLQPPDPLSVKNAIELLKTIGALDDLEELTYLGQHLCTLPLDPNIGKMLLIGSVFQCLDPALTIAAALAYRNPFVLPIDRKEEADAVKRSFAGDSCSDHIALLKAFEAWKDAKHSGRERSFCWENFLSPMTLKMMDDMRNQFFDLLSDIGFVSKTRGVKAYNHYGKDLEMVSAVLCAGLYPNVIQCKKRGKRTAFYTKDVGKVDIHPSSVNAGVQQFPLPYLVYSEKVKTASIYVRDSTNISDYALLLFGGSLSPSKTGEDIEMLGGYLHFSAPKRIIELIQRLRGELDKLLQRKIEEPALDIFSEGKGVVAAAIELLHSQNVYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R2Z7</accession>
    <name>A0A0Q3R2Z7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g38020v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R2Z7"/>
    <dbReference type="FunCoup" id="A0A0Q3R2Z7">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK07850">
        <property type="protein sequence ID" value="KQK07850"/>
        <property type="gene ID" value="BRADI_2g38020v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07850">
        <property type="protein sequence ID" value="KQK07850"/>
        <property type="gene ID" value="BRADI_2g38020v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3R2Z7"/>
    <dbReference type="OrthoDB" id="695856at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050232">
        <property type="entry name" value="FBL13/AtMIF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900">
        <property type="entry name" value="F-BOX/RNI SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900:SF30">
        <property type="entry name" value="SUPERFAMILY PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="2">
        <location>
            <begin position="324"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK07850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="654" mass="71953" checksum="CEEC122F8367A559" modified="2016-01-20" version="1">MAGDRLSSLGEGALGRVLSFLPSSKEAARAAALSSRWRDAFAGADAVSMEEPERPLRPSDYEDRGCGCMDCGFGKPVDPNPPPPFATAVTAALLARHRRSGPDAPPLRALRVALNCYRREDASQVDHWVSYALKHTAAAGGLEICLRLRRGAVCGRKYSLVARREAGSLEEKDEEDFDVDCEEEPRGRTRSRSQSMAEDSDEEEADRRAMPRRRRSVSGDEMDDAAASDYDNHFADSPPPRPRQPSAPRSPDSRSSSNSDDPAGADRGRSPTPHGLSPPRSRGYRSPTPPRSPSQTPSPGEDDDAVSDAEDSRSRPDWPVPSWERPPSPEYTVPRTLISCAVLRSLTIGPCSLSPPAIISLPSLETLLLTDVSDEEEDVQRLVAACPRLADLTLEACGTVTALSLLRNRHLRRLALRCCHALATVAINGTLLRSFEYRGAVPDASLLTFHGGRSSFVSCKVDICGEETRHLHLQSARLGSGVDHHAMALPAFTNLRRLELTGRLPHDDDDGASLAAVSRILRRAPRLEALSLFFDMGPSDDDELRERQSWRDTNEAALLDSHHLKYNEHEVLLDVVAPAAAVPPCLRNRVREINLVHYHGGRAQRTLAKFLLCNALVLDRLYCGFARGPLWIQTELRDEIRGWAMNKAENIFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R9W8</accession>
    <name>A0A0Q3R9W8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4-coumarate--CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100830101</name>
        <name evidence="10" type="ORF">BRADI_2g50557v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(E)-4-coumarate + ATP + CoA = (E)-4-coumaroyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19641"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19642"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(E)-4-coumarate + ATP + H(+) = (E)-4-coumaroyl-AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:72419"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:72420"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(E)-4-coumaroyl-AMP + CoA = (E)-4-coumaroyl-CoA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:72423"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:85008"/>
            <dbReference type="ChEBI" id="CHEBI:192348"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:72424"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.12" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754841.1">
        <property type="nucleotide sequence ID" value="XM_014899355.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R9W8"/>
    <dbReference type="STRING" id="15368.A0A0Q3R9W8"/>
    <dbReference type="EnsemblPlants" id="KQK09858">
        <property type="protein sequence ID" value="KQK09858"/>
        <property type="gene ID" value="BRADI_2g50557v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830101"/>
    <dbReference type="Gramene" id="KQK09858">
        <property type="protein sequence ID" value="KQK09858"/>
        <property type="gene ID" value="BRADI_2g50557v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830101"/>
    <dbReference type="OrthoDB" id="2962993at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3R9W8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016207">
        <property type="term" value="F:4-coumarate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106290">
        <property type="term" value="F:trans-cinnamate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05941">
        <property type="entry name" value="MCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RSS9</accession>
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        <name type="scientific">Brachypodium distachyon</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="1">Bd21</strain>
        </source>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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            <dbReference type="EMBL" id="KQK16130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16130"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQK18554">
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    </dbReference>
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        <property type="gene ID" value="BRADI_1g43255v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="653237at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF430">
        <property type="entry name" value="OS06G0295000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        </location>
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            <end position="21"/>
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        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="458" mass="51560" checksum="7EBC0E71652675D0" modified="2016-01-20" version="1">MESQPQPGNGHELQNTTSQQEPHQHDDQTPSSADEAELLWKLRKYLILLAILSATITYQAGLAPPGGLWQDNQHGHIASDIVLQYSYTKRYKVFFYCNTTAFMASFIVLILLLAAGSCREVRTSVYIWILLVGIFTYVALHVVFFRHLAPEWLREIFRDVQRFWDESIAHIFIRRHNVTGEPDAPIQDKKEELEQNRSFLLVAATLVAAVTYSAGLSPPGGFWPNNNASHIAGSCREIRTSIFVLTLVGAVLLYLIIQWVAPIVRKPEFVQKSIKWIKENKITYQAGLNPPGGFWPNSRDEHLAGDPILEAINPMRYNAFFYCNANAFVSSLVIITLLQSQLITVGAMKRITIEASKLTEKLGKWRKDLMLVGTFAVSVTYQAGLLPPGDPVIHDTHPMRYKMFFYCNATAFMASMVIVILLLNSTMSKYRRSLLAMKTAMVMDLVCLGRMLQAAAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3S9T3</accession>
    <name>A0A0Q3S9T3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g62574v3</name>
    </gene>
    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3S9T3"/>
    <dbReference type="EnsemblPlants" id="KQK21706">
        <property type="protein sequence ID" value="KQK21706"/>
        <property type="gene ID" value="BRADI_1g62574v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21706">
        <property type="protein sequence ID" value="KQK21706"/>
        <property type="gene ID" value="BRADI_1g62574v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3S9T3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK21706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="166" mass="17896" checksum="4EAD212CFD4681D1" modified="2016-01-20" version="1">MCSRPCAFAVSGLGRPLGAAHTRDSSRTTPYPLLAHEQAGRVVRTAGPFLHKISPHHDPAPLPPQQPSPGRRAGARPEQLVLKNSSSHGARRSPSSSRSTRFWPRRPRAPPGLRRAPAPLRRPPLPSALDGNISVRLPSGALALALRRPSMDCAVLLAFASFFFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CV75</accession>
    <name>A0A2K2CV75_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g04496v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CV75"/>
    <dbReference type="EnsemblPlants" id="PNT65924">
        <property type="protein sequence ID" value="PNT65924"/>
        <property type="gene ID" value="BRADI_3g04496v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65924">
        <property type="protein sequence ID" value="PNT65924"/>
        <property type="gene ID" value="BRADI_3g04496v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CV75"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT65924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="103" mass="11375" checksum="F28B190C43C6D474" modified="2018-03-28" version="1">MYAAFCPLQLPSSLINHTDQTFISKYTQTTSILSIFLSTAILILEASSLHCFSGARSNGRFSQHCVCSVIKIPHLSWITCEVFNQKRAYVGGNAQLFVGIALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYP2</accession>
    <name>A0A2K2CYP2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g21483v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CYP2"/>
    <dbReference type="EnsemblPlants" id="PNT67148">
        <property type="protein sequence ID" value="PNT67148"/>
        <property type="gene ID" value="BRADI_3g21483v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67148">
        <property type="protein sequence ID" value="PNT67148"/>
        <property type="gene ID" value="BRADI_3g21483v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CYP2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT67148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="65" mass="7568" checksum="59263DCB36D32039" modified="2018-03-28" version="1">MIFLFEQSHHSDCCLPIFWQTLRLPHDPVAHATRSHHQSSMPATRSVVQDSRVLSLNMFHWKALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CZF0</accession>
    <name>A0A2K2CZF0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">At1g68980-like TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_3g27027v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CZF0"/>
    <dbReference type="FunCoup" id="A0A2K2CZF0">
        <property type="interactions" value="971"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CZF0"/>
    <dbReference type="EnsemblPlants" id="PNT67400">
        <property type="protein sequence ID" value="PNT67400"/>
        <property type="gene ID" value="BRADI_3g27027v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67400">
        <property type="protein sequence ID" value="PNT67400"/>
        <property type="gene ID" value="BRADI_3g27027v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CZF0"/>
    <dbReference type="OrthoDB" id="726314at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CZF0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057440">
        <property type="entry name" value="At1g68980-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46598">
        <property type="entry name" value="BNAC05G43320D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46598:SF1">
        <property type="entry name" value="OS10G0422566 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25245">
        <property type="entry name" value="TPR_At1g68980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="At1g68980-like TPR repeats" evidence="5">
        <location>
            <begin position="79"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="512"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="619"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT67400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="780" mass="87994" checksum="46E3AAC08C8381B3" modified="2018-03-28" version="1">MLRVGRVCTRSNPCRALISFFVSPLPLVGHDSVHFLDYVRNRTKRTADKNIGFLNHKLHIRSCGACFSTTAQTVLVQARDSSQLALEIENAIDQQRFDDAWRAYEKHVHMDGLPRKSVLSKLITGFAVTRDVHRLNQSYNVVDHAFEEKHELLEKEPLIYLSLTLAQCALPNLAVNVVRKLVKMEAYPPVAAWSGIIAHMCQTANGAFLAADMVMEIGYLFQNNRVDPRKKSNRPLLSMKPNSFTFNIVLTASLLFGTTKKAEQLLELMPRIGVKPDVSLLIVMARIYEKNGHKDEIVKLKRHVDEACGLSELEFQQFYDCLLSCHLKFGDLDSAVDMVLDMLRKGKNAKRSLEAAKAVLEAVESSKIYLPFEKSDPENSGSPNKSVSANGQMRSYVSFFKDKSFARLELDARGLLNLLSDKLQDQVGLVKSEHGILHPTETMYAKLVKAFLEADKISALASFLVKASKEDSPVSVESSFVVQVINACISVGLLEQAHDLLDEMRFSGIKVGSSIYSSLLKAYCKEGHHEDDITALLKDAQQAGIQLDSSCYEDLIQSRVHHKNTAGALHLFKEMRNSKVVKSGHREFEMLVQSCDNSEAALTTKLVEEVKSGHTVNHAIHDWNNVIHFFCKKRLMHDAHKALSKMRVLGHTPNAQTFHSLVTAYAAIGGKYVEVTDLWGEMKVLANSNSMKFDQELLDSLLYCFVRGGFFLRAMEVIEMMEKCKMFIDKYKYKSLWLKYHRTMYKGNPTTEMSELLLHCAVWRCSGISIVGYHCMSHLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D0S8</accession>
    <name>A0A2K2D0S8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glucan endo-1,3-beta-D-glucosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_3g33277v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
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        <property type="protein sequence ID" value="PNT67880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D0S8"/>
    <dbReference type="STRING" id="15368.A0A2K2D0S8"/>
    <dbReference type="EnsemblPlants" id="PNT67880">
        <property type="protein sequence ID" value="PNT67880"/>
        <property type="gene ID" value="BRADI_3g33277v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67880">
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        <property type="gene ID" value="BRADI_3g33277v3"/>
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    <dbReference type="OrthoDB" id="1938138at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2D0S8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="GH"/>
    </dbReference>
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        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
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        <location>
            <position position="1"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT67880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="30152" checksum="5135E11F8EF1BFD2" modified="2018-03-28" version="1" fragment="single">SLTNSLLPSLADKSTGLTTAVSWIRANLATPATNVTLLLAGNEILPSPDPNLILSLLPAMRRLAQALKSEGFPSIRVSTPHYLGILAPSDGIPTNATFRAGYNTKLFPSMPQFHRETKPPFMVNPYPYFSYNPQTLNYTLFRPNARIYDPATKLNYTSMFEAQMDAIYTAMVKLGYGDVEVAIGEAGWPTQAEADQVGVGVKEAQDFNEGMLRVCSSGKGTPLMPNRTFETYLFSLFDENQKPGPVDERHFGLFNPDFTPVYDLRLLTDGKGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D145</accession>
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        </submittedName>
    </protein>
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="BRADI_3g34713v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g34713v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D145"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT68006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="99" mass="11382" checksum="EB30159FD495F7C7" modified="2018-03-28" version="1">MTSIFFLLEYIGVSSIKEGELRCSLQALRAVITNYSTRVSPLSTYHSWCKAKTPKMLQMKGVLIFESGKTERRPMKMHEESKCKFLEVTNKVLLPSHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D4D6</accession>
    <name>A0A2K2D4D6_BRADI</name>
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        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="1">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
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                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT69137">
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        <property type="gene ID" value="BRADI_3g50085v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69137">
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        <property type="gene ID" value="BRADI_3g50085v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D4D6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT69137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="32" mass="3469" checksum="321714FF6008D9A0" modified="2018-03-28" version="1">MGVAGHQARSYRLCDYDSCANRSPCSMATTIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DB24</accession>
    <name>A0A2K2DB24_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT71462">
        <property type="protein sequence ID" value="PNT71462"/>
        <property type="gene ID" value="BRADI_2g27886v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71462">
        <property type="protein sequence ID" value="PNT71462"/>
        <property type="gene ID" value="BRADI_2g27886v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DB24"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="53" mass="6237" checksum="6FC5D36A04C37093" modified="2018-03-28" version="1">MSSATTMENPSPDFYRHHVLAPPELCFRQVRSKPELPSWRDPNLREENRVKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DD20</accession>
    <name>A0A2K2DD20_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g40925v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DD20"/>
    <dbReference type="EnsemblPlants" id="PNT72183">
        <property type="protein sequence ID" value="PNT72183"/>
        <property type="gene ID" value="BRADI_2g40925v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72183">
        <property type="protein sequence ID" value="PNT72183"/>
        <property type="gene ID" value="BRADI_2g40925v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DD20"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT72183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="99" mass="9902" checksum="78D6D9E927D8BFF4" modified="2018-03-28" version="1">MGIYPPEETDLAAPTRKPGGAAAPRRLAGWSQWLSAAELASGHDGGSVAGAAALELNSFGGDILSNLPGFVAGTAALEVPKSALSSPAADSTVILNWTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DE79</accession>
    <name>A0A2K2DE79_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g46880v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DE79"/>
    <dbReference type="FunCoup" id="A0A2K2DE79">
        <property type="interactions" value="905"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DE79"/>
    <dbReference type="EnsemblPlants" id="PNT72604">
        <property type="protein sequence ID" value="PNT72604"/>
        <property type="gene ID" value="BRADI_2g46880v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72604">
        <property type="protein sequence ID" value="PNT72604"/>
        <property type="gene ID" value="BRADI_2g46880v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DE79"/>
    <dbReference type="OrthoDB" id="1939205at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DE79">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034604">
        <property type="entry name" value="SRRP53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31968">
        <property type="entry name" value="SERINE/ARGININE-RELATED PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31968:SF4">
        <property type="entry name" value="SERINE_ARGININE-RELATED PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="24"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT72604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="338" mass="37651" checksum="5ACC2806B768CFB1" modified="2018-03-28" version="1">MEEEKAAAYYDELNRKGEGARRFKQGLGFSSGDPQPAFSSKTTNTSSSSFLSGFVRAGANPAQLPNPTKPHPPREAPSRHRRRSPSPSRPRPGSRSPSRSWRRRSRSRERGDRRASHHRSRSRSPSRRSGRSSNARDRHGDRRRDDGGSGGRESSKVRGGKDGGKVDYSRHIDGYDKMVGPVLRLRLTPAERVKAKMKLQLSETASKDSTLGNATVGWERFEFNKDAPLDEDDADVEAAHDDASLVKHIGKSFRLSAVESKHENLVRDAHDEAMFGVPMSSIVGTEASEDELKTNYEAKKAEDVETQPSSSLISDKVLAMQKVSWRERIQKLRQSSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DFT8</accession>
    <name>A0A2K2DFT8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100845586</name>
        <name evidence="20" type="ORF">BRADI_2g54240v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Calcium-dependent NADPH oxidase that generates superoxide.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RBOH (TC 5.B.1.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DFT8"/>
    <dbReference type="EnsemblPlants" id="PNT73148">
        <property type="protein sequence ID" value="PNT73148"/>
        <property type="gene ID" value="BRADI_2g54240v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73148">
        <property type="protein sequence ID" value="PNT73148"/>
        <property type="gene ID" value="BRADI_2g54240v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000026">
        <property type="entry name" value="Putative respiratory burst oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000049">
        <property type="entry name" value="Respiratory burst oxidase homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000019">
        <property type="entry name" value="Respiratory burst oxidase homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_b245_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013623">
        <property type="entry name" value="NADPH_Ox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF54">
        <property type="entry name" value="RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN J-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08414">
        <property type="entry name" value="NADPH_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01169">
        <property type="entry name" value="NADPH_oxidase_subgroup_(NOX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="490"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="536"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="725"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="233"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="19">
        <location>
            <begin position="578"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="PNT73148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="907" mass="101554" checksum="526241A3C99B6BA5" modified="2018-03-28" version="1">MLPAISVSFLPPSPPLQRICSSSRLARPPHLSPFTGGVPPPAAGAAGIVLSEGRSVWITTDGMASPGGGYSDRPPALDGITVEGASGRPPLPRPPGFARGLLKQPSRLASGVRQFASKVSMKVPEGVAGPWGAGRNMTRMQSSAQMGLRGLRFLDKTSGGKEGWKAVERRFEEMNKAGRIPKESFGKCIGMGDSKEFAGELFVTLSRRRHIEPEEGINKEQLKEFWEEMTDQNFDSRLRIFFDMCDKNGDGMLTEDEVKEVIILSASANKLAKLKGHAATYASLIMEELDPDDRGYIEIWQLETLLRGMVSAQAPEAKLKRTTSSLARTMIPSRYRSPLKRHVSRTVDFVHENWKRIWLVTLWLAVNLALFVYKFEQYKRRSSFQVMGYCVCIAKGAAETLKLNMALILLPVCRNTLTTLRSTALSRVIPFDDNINFHKVLAGAIAVATTVHTLAHVTCDFPRLVSCPSDKFMATLGPNFSFRQPTYPDLLASAPGVTGILMIIIMSFSFTLAMHSFRRSVVKLPSPLHHLAGFNAFWYAHHLLVLAYVLLVVHSYFMFLTRVWYKKTTWMYLIVPVLFYACERIIRKVRENNYRVSILKAAIYPGNVLSLHMKKPPGFKYKSGMYLFVKCPDVSPFEWHPFSITSAPGDDYLSVHIRTLGDWTSELRNLFGKCCQAQVTSKKATLSRLETTVVADSQTEDTRFPKVFIDGPYGAPAQNYKKYDILLLIGLGIGATPFISILKDLLNNIKSNEEVESIHGSEIGSFKNTGPGRAYFYWVTREQGSFDWFKGVMNDVADSDHTNVIEMHNYLTSVYEEGDARSALIAMVQSLQHAKNGVDIVSGSKIRTHFARPNWRKVFSDLANAHKNSRIGVFYCGSPTLTKQLKDLSKEFSQTTTTRFHFHKENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DIL9</accession>
    <name>A0A2K2DIL9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100831895</name>
        <name evidence="1" type="ORF">BRADI_1g08370v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752498.1">
        <property type="nucleotide sequence ID" value="XM_014897012.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DIL9"/>
    <dbReference type="STRING" id="15368.A0A2K2DIL9"/>
    <dbReference type="EnsemblPlants" id="PNT74125">
        <property type="protein sequence ID" value="PNT74125"/>
        <property type="gene ID" value="BRADI_1g08370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831895"/>
    <dbReference type="Gramene" id="PNT74125">
        <property type="protein sequence ID" value="PNT74125"/>
        <property type="gene ID" value="BRADI_1g08370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831895"/>
    <dbReference type="OrthoDB" id="1724864at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DIL9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT74125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="24886" checksum="CA702FC5DB6449F4" modified="2018-03-28" version="1">MCGDVPPPICRPAAGPAVASPPPRSPLSALASAFPPRIHCCRPFTVRRHPPAATIDQAHRTASLLPQLVCACATVEEEGDADPRVLAPFVEAWEEEQDDGMDLRLCLRGEYGRCVAMVLDSAVLCESSAFSAVGGKEIEVYGVENMAALRDAVEFMFDADAPRWLARAGVSRAIAVLECCQEKLAGYISKGMISTITRREENIYGLPIWSKQIIGICCSSTEIKLTTASSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DJN2</accession>
    <name>A0A2K2DJN2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcription factor GAMYB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">OsGAMyb</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100826911</name>
        <name evidence="16" type="ORF">BRADI_1g15581v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751997.1">
        <property type="nucleotide sequence ID" value="XM_014896511.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DJN2"/>
    <dbReference type="SMR" id="A0A2K2DJN2"/>
    <dbReference type="EnsemblPlants" id="PNT74484">
        <property type="protein sequence ID" value="PNT74484"/>
        <property type="gene ID" value="BRADI_1g15581v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826911"/>
    <dbReference type="Gramene" id="PNT74484">
        <property type="protein sequence ID" value="PNT74484"/>
        <property type="gene ID" value="BRADI_1g15581v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826911"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DJN2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009555">
        <property type="term" value="P:pollen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000119">
        <property type="entry name" value="Transcription factor GAMYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47995">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47995:SF18">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0287">Flowering</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="15">
        <location>
            <begin position="46"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="14">
        <location>
            <begin position="46"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="15">
        <location>
            <begin position="99"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="14">
        <location>
            <begin position="99"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="264"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="354"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="PNT74484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="459" mass="48820" checksum="52F49E89195504B5" modified="2018-03-28" version="1">MPMDQGKSSDMVAAPASPEEENAAATGDSSDEEAPQNQMDNEQAPAAALKKGPWTAAEDTMLMNHVLQHGEGNWNAVQRMSGLLRCGKSCRLRWTNHLRPNLKKGSFSPQEELLIAQLHAQLGNKWARMASHLPGRTDNEIKNYWNTRCKRRQRAGLPVYPPEVQLQLATAAKRCRYDDFSGPLSSSSPQESGSSGNVLSLGLGAPDAAAGSYTSARPAALDLAMGTRPVQFLNAPTMPFSAPSTPWSTTPPFQRNARYLKLSSAPVSPTGAPPVHPVTPELSLGGLPGTARFPPLSPVPGAARVELPSSQLRPAMQQPTPAPGGQQAASATAGVPQYQQNGTSYEEILQGLNDAIKPPENGSSVDANGQQQEEADGVSGGNNGSVDALKHDLDTLFGFAVMTPATPFPTAEPDALDVVAANNSGWISQHSSDEQDNSIIDQDWGLDGTFQWNSMSRIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DMY6</accession>
    <name>A0A2K2DMY6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g35896v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DMY6"/>
    <dbReference type="EnsemblPlants" id="PNT75633">
        <property type="protein sequence ID" value="PNT75633"/>
        <property type="gene ID" value="BRADI_1g35896v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75633">
        <property type="protein sequence ID" value="PNT75633"/>
        <property type="gene ID" value="BRADI_1g35896v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DMY6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="116" mass="13047" checksum="B0281F3C4E12AF1A" modified="2018-03-28" version="1">MTAAQSRKVYTIYDVCTRQHFPMQYAHLATLVVSFPDRDRRRRNIAAAAAKPDAGRQRQGQEPKSGLESGRKDQNSESEGFASLCHCPGAGNLLFRSGTSRTCIFNYELTIHNWVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DPA2</accession>
    <name>A0A2K2DPA2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-ketoacyl-CoA synthase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100838880</name>
        <name evidence="9" type="ORF">BRADI_1g44000v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313672.1">
        <property type="nucleotide sequence ID" value="XM_024457904.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313673.1">
        <property type="nucleotide sequence ID" value="XM_024457905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DPA2"/>
    <dbReference type="STRING" id="15368.A0A2K2DPA2"/>
    <dbReference type="EnsemblPlants" id="PNT76099">
        <property type="protein sequence ID" value="PNT76099"/>
        <property type="gene ID" value="BRADI_1g44000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838880"/>
    <dbReference type="Gramene" id="PNT76099">
        <property type="protein sequence ID" value="PNT76099"/>
        <property type="gene ID" value="BRADI_1g44000v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DPA2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="FAE" evidence="7">
        <location>
            <begin position="72"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="8">
        <location>
            <begin position="395"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036417-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT76099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="501" mass="54665" checksum="5C71329C9A18DE66" modified="2018-03-28" version="1">MHRVPPPKDSSTMAISLALNLFTRRLTLAAFPWPFSATVVQLSSNGISIALLHAGLTLLAAALLLYYYYYITSTRARTVYLVDYACFLPPASLRVPLAKYAEHAGLAPCFDDRSVGFQSRLIERSGLGEETCLPQASHGIPPEKTLDAARAEAEQGIFSAVDAVLAKTVVRAEDIALVVVNCTLFAPAPCMADMVVRRYALRSDVRCFNLSGMGCSAGIAAVGLAQNVLMHCSGSGGGSGRYALVVSTEILTYDYYSGNERAMLLQNCLFRMGASAVLLSNSRRSSPARYRLERVVRTHEGHDDGAYGCVQQEDDAAGERGIKLSKEVMPVAGRALRAHMTTLGPLVLPASELLAYVLSLARRRLLLLIFAGQLKDRKPAPPGRVDVPDFRRAFEHFCIHAGGRAVIDELQRGLGLSDRQVEPSRMALHRFGNTSSSSVWYELAYLEAKRRVRRGDRVWTVGFGSGFKCNSAVWVCLRPPAPLDSGPWDACIHRYPVATVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DSE7</accession>
    <name>A0A2K2DSE7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g58976v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DSE7"/>
    <dbReference type="EnsemblPlants" id="PNT77194">
        <property type="protein sequence ID" value="PNT77194"/>
        <property type="gene ID" value="BRADI_1g58976v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77194">
        <property type="protein sequence ID" value="PNT77194"/>
        <property type="gene ID" value="BRADI_1g58976v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DSE7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT77194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT77194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="215" mass="22504" checksum="2C2FE34BF9892DD5" modified="2018-03-28" version="1">MRRRRRGRNCPTTGLGVRARRALEAPLRVSPGVRPLSAAQPFSATAGRPVGQTLFQTPFSPASRAASRRSRGRRSSSPGTSTELRIWLARTSFGATGSFLHSAQLNFHSQFLDFSSSSSLVTSTKSSTSRSFPSVPPAFPPPPDGFAASFVAASSYPVAQLSMQCQSALPLGSFSRSDFASAAASPASLMAAAMAASEMDLGSVAEEAEARSSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1GKW4</accession>
    <name>I1GKW4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100828570</name>
        <name evidence="11" type="ORF">BRADI_1g01740v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562360.1">
        <property type="nucleotide sequence ID" value="XM_003562312.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GKW4"/>
    <dbReference type="FunCoup" id="I1GKW4">
        <property type="interactions" value="3029"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GKW4"/>
    <dbReference type="EnsemblPlants" id="KQK12123">
        <property type="protein sequence ID" value="KQK12123"/>
        <property type="gene ID" value="BRADI_1g01740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828570"/>
    <dbReference type="Gramene" id="KQK12123">
        <property type="protein sequence ID" value="KQK12123"/>
        <property type="gene ID" value="BRADI_1g01740v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828570"/>
    <dbReference type="eggNOG" id="KOG2307">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005470_0_0_1"/>
    <dbReference type="OMA" id="CWAEGVY"/>
    <dbReference type="OrthoDB" id="332281at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060178">
        <property type="term" value="P:regulation of exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009316">
        <property type="entry name" value="COG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024603">
        <property type="entry name" value="COG_complex_COG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024602">
        <property type="entry name" value="COG_su2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12022">
        <property type="entry name" value="COG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06148">
        <property type="entry name" value="COG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 2 N-terminal" evidence="9">
        <location>
            <begin position="30"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="COG complex component COG2 C-terminal" evidence="10">
        <location>
            <begin position="414"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK12123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="759" mass="83825" checksum="1EA15475E350F06D" modified="2012-06-13" version="1">MADLAVASPPAAAAAATDLFGEPIEAHPPWFRPDAFLRAGFDPDAYVTELRSYVPLESLAAELRAHLAALRAELVGLINRDYADFVGLSARLKGVDAAAARMRAPLAELRDKVAAFRAAASAALAALRAGLEQRAAAAAARELLELLLDTSHVVSKVEKLIKELPTAPSDSSNAEVVSVDKGYSGNDVTPSNVEARTDVRETQSILVERIASEMNRLKFYISHAQNLPFIENMEKRVQGATKLLDVSLERCFVIGLEHRDAKVIYNCLRAYAAIDNTSSAEELFRSTVVSPLIQNIMPLNYAKAVSGVASDDLEDDYQRIMQCVEKDCKFILEISSSANSGLHIFDFLANSILKEVHSAIMKGKPGACSPGKPKDFLKNYKASLRFLDFLEGYCPSKSAVTKFRSEPAYTDFMRQWHVSVYFSLRFQEIAGGLDSALTTTISPVGMNENQTRQKKLLLKQSVKLLESLHSCWSDEVLVFSHSDKFLRLSLQLISRYTTWLSSGLAARKAADGSSSSPADAEWALSVPVEDFIYVMHDVNAVIGELSESGNFVGHVNQLLASCPIEVLTLVKQSILQAVEPLKEVLPGIMDVMIGVIVKRSNEDLKHLKGITATYRMTNKLPVRHSPYVSGILHPLKVFLEGDRVHYLSEDDKTKLRCGSTDKITAIYFDMVSEVVNVARKTESSLQRLRQGAQRRVGASTDASDNVISDTDKICMQLFLDIQEYARNLRLLGIDAREIESYRALWQCVAPKDKQDLIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1GKX3</accession>
    <name>I1GKX3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100846376</name>
        <name evidence="3" type="ORF">BRADI_1g01820v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562420.1">
        <property type="nucleotide sequence ID" value="XM_003562372.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GKX3"/>
    <dbReference type="STRING" id="15368.I1GKX3"/>
    <dbReference type="EnsemblPlants" id="KQK12137">
        <property type="protein sequence ID" value="KQK12137"/>
        <property type="gene ID" value="BRADI_1g01820v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846376"/>
    <dbReference type="Gramene" id="KQK12137">
        <property type="protein sequence ID" value="KQK12137"/>
        <property type="gene ID" value="BRADI_1g01820v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846376"/>
    <dbReference type="eggNOG" id="ENOG502S24M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114322_0_0_1"/>
    <dbReference type="OMA" id="DPIRGWD"/>
    <dbReference type="OrthoDB" id="678664at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009860">
        <property type="term" value="P:pollen tube growth"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044509">
        <property type="entry name" value="RIC2/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46931">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN RIC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46931:SF17">
        <property type="entry name" value="OS03G0847900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="2">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK12137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="155" mass="16100" checksum="760C9442212FA89C" modified="2012-06-13" version="1">MKDRRGAGFPFSIGCMSQSAVAVADPLEKKQTQADPPSSSTTSNTTTTTHGAGEEGGSGGEEKAVAPGIVAAGVQRLIKGIKMSLSQMFATDGNGEEEEEEEREIVIGYPTDVQHVGHIGWDGLNKVGGMGMVSAFSLPSSLSLRQLEIAMEGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>I1GL73</accession>
    <name>I1GL73_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Flap endonuclease 1</fullName>
            <shortName evidence="15">FEN-1</shortName>
            <ecNumber evidence="15">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100821896</name>
        <name evidence="19" type="ORF">BRADI_1g02697v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">Bd21</strain>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. May be required for cell proliferation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Mitochondrion</location>
        </subcellularLocation>
        <text evidence="15">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13 15">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="15"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239175.2">
        <property type="nucleotide sequence ID" value="XM_010240873.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758151.1">
        <property type="nucleotide sequence ID" value="XM_014902665.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GL73"/>
    <dbReference type="STRING" id="15368.I1GL73"/>
    <dbReference type="EnsemblPlants" id="KQK12287">
        <property type="protein sequence ID" value="KQK12287"/>
        <property type="gene ID" value="BRADI_1g02697v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821896"/>
    <dbReference type="Gramene" id="KQK12287">
        <property type="protein sequence ID" value="KQK12287"/>
        <property type="gene ID" value="BRADI_1g02697v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821896"/>
    <dbReference type="eggNOG" id="KOG2519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032444_2_0_1"/>
    <dbReference type="OMA" id="IQEVHID"/>
    <dbReference type="OrthoDB" id="1937206at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09907">
        <property type="entry name" value="H3TH_FEN1-Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000015">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF56">
        <property type="entry name" value="FLAP ENDONUCLEASE 1-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1 15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="18">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exonuclease" evidence="16">
        <location>
            <begin position="28"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="17">
        <location>
            <begin position="147"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQK12287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="413" mass="46166" checksum="25F39AEAB1CBCF83" modified="2012-06-13" version="1">MGIKGLTKLLAEHAPRAAAQRRVEDYRGRVIAIDASLSIYQFLVVVGRKGTEVLTNEAGEVTSHLQGMLNRTVRLLEAGIKPVFVFDGEPPDLKKRELAKRSLRRDDASEDLNRAIEVGDEDSIEKFSKRTVKITKKHNDDCKKLLRLMGVPVVEAPGEAEAQCASLCKNHKAYAVASEDMDSLTFGSLRFLRHITDLSFKRSPVTEFEVPKVLEELGLTMDQFIDLCILSGCDYCENIKGIGGQRALKLIRQHGCIEEVVQNLNNRFTVPEDWPYQEVRTLFKEPNVCTEIPDFQWTSVDKEGIVNFLAIENSFSSDRVEKAVQKIKAARDRYSPGRVKLLTPVANLSGSITKKEPECVLGSSGQGLKSWSALQVCRSSSSDFRYRSYYSSKPLVLGRQSGFHGIPHAFSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1GLA7</accession>
    <name>I1GLA7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine-threonine/tyrosine-protein kinase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_1g02970v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GLA7"/>
    <dbReference type="STRING" id="15368.I1GLA7"/>
    <dbReference type="EnsemblPlants" id="KQK12327">
        <property type="protein sequence ID" value="KQK12327"/>
        <property type="gene ID" value="BRADI_1g02970v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12327">
        <property type="protein sequence ID" value="KQK12327"/>
        <property type="gene ID" value="BRADI_1g02970v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1268458_0_0_1"/>
    <dbReference type="InParanoid" id="I1GLA7"/>
    <dbReference type="OrthoDB" id="781197at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002518">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052101">
        <property type="entry name" value="Plant_StressResp_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983:SF20">
        <property type="entry name" value="OS03G0844100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983">
        <property type="entry name" value="PTO-INTERACTING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK12327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24703" checksum="AF31C5340A924915" modified="2012-06-13" version="1">MCIISGSSSDNFRCRACRPGVETVSDLKKLFSFAEQYGYADWICFDASVIFYDWPVDTEEGCLQQYSFGVVLLELLTGRKPVDHTMPRGQQNLVTWATPRLSEDKVKQCIDPRLKGEYPPKGWFRLAAVAALCVQYEAEFRPNMSIVLTVAPGAKIRRQLAMVYMRLLRKSRCISIPSPIPVLCDSVYSIHLTDKLNVPIVRCPYEETGNILLLDGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GME0</accession>
    <name>I1GME0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100846857</name>
        <name evidence="14" type="ORF">BRADI_1g06020v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559336.1">
        <property type="nucleotide sequence ID" value="XM_003559288.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GME0"/>
    <dbReference type="STRING" id="15368.I1GME0"/>
    <dbReference type="EnsemblPlants" id="KQK12795">
        <property type="protein sequence ID" value="KQK12795"/>
        <property type="gene ID" value="BRADI_1g06020v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846857"/>
    <dbReference type="Gramene" id="KQK12795">
        <property type="protein sequence ID" value="KQK12795"/>
        <property type="gene ID" value="BRADI_1g06020v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846857"/>
    <dbReference type="eggNOG" id="ENOG502S3NY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058719_4_0_1"/>
    <dbReference type="OMA" id="YKADQEA"/>
    <dbReference type="OrthoDB" id="784190at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009610">
        <property type="term" value="P:response to symbiotic fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000067">
        <property type="entry name" value="Cupredoxin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF441">
        <property type="entry name" value="OS03G0791366 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093941" description="Phytocyanin domain-containing protein" evidence="12">
        <location>
            <begin position="25"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="13">
        <location>
            <begin position="25"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK12795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="164" mass="17196" checksum="35E0B6341F158B35" modified="2012-06-13" version="1">MASKHMLVAVVALAAAFLPGLAVASEHVVGDDKGWTLQFNYTAWSESRQFVVGDTLLFKYGSSAHNVVEVGGADFMACTKPPTANTWSTGEDRVTLDKAGRRWFICDIGEHCEKGGMKFKVTVNEAGAPSPNSPPAPSNPAGKVHAKHFGVAAVTALAAAALVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1GN34</accession>
    <name>I1GN34_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100840566</name>
        <name evidence="12" type="ORF">BRADI_1g08010v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752106.1">
        <property type="nucleotide sequence ID" value="XM_014896620.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GN34"/>
    <dbReference type="STRING" id="15368.I1GN34"/>
    <dbReference type="EnsemblPlants" id="KQK13094">
        <property type="protein sequence ID" value="KQK13094"/>
        <property type="gene ID" value="BRADI_1g08010v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840566"/>
    <dbReference type="Gramene" id="KQK13094">
        <property type="protein sequence ID" value="KQK13094"/>
        <property type="gene ID" value="BRADI_1g08010v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840566"/>
    <dbReference type="eggNOG" id="KOG0242">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013407_0_0_1"/>
    <dbReference type="OMA" id="PQAHHER"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01374">
        <property type="entry name" value="KISc_CENP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000016">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021881">
        <property type="entry name" value="NACK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF19">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11995">
        <property type="entry name" value="DUF3490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="32"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="615"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="395"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="478"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="636"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="117"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK13094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="948" mass="107681" checksum="80CBAE25D9DC0B7E" modified="2012-06-13" version="1">MGASRHPSTPASKIARTPSLTPGGSSRSKEEKIFVTVRVRPLSKKELAVKDQVAWECADRQTILYKGPTQDRAAPTSYTFDKVFGPGCKTDLVYEDGAKDVAMSAMTGINATIFAYGQTSSGKTFTIRGVTESAVSDIYRHIENTPEREFIIKISAMEIYNEIVKDLLQPDSGPLRLLDDPEKGTIVEKLEEKIADDRQHLRHLIDICEEQRQVGETALNEASSRSHQIIRLTVESRLREVSDCVKSLVASLNFVDLAGSERAAQTHAIGARLKEGCHINRSLLTLTTVIRKLSSEKRSGHVPYRDSKLTRILQLSLGGNARTAIICTMSPARTHIEQSRNTSFFAACAKEVTNTAKVNMVVTDKQLVKHLQTEVARLEAVLRTPDRASSSEIAIMEKDRKLRLMEKEMEELKKERDNARAELEELRKKMGDNQQGWNLFDSPQMARKCLTFSGSLHSSKKIKIRNSIRQSSTAPFMLKHEIRKLEQLQQQLEVEANRAIEVLHKEVECHKQGNQDAAETVAKLQAEIREMQSVRSENTDVEMVADEGNGSDLKDEITRLHMQDTDIAKLEAKLENVQRSIDKLVMSLPNVVTQCNGTTTKFSVSKKKRRMLLPLGMSNINRPNLIRAPCSSSRPSESEPENRAPESDLVCHEDQGKATPTKSEDTGDVSSRDETPRNRRSSSVNMKKMQRMFQNAAEENVRNIRDYVTELKERVAKLQYQKQLLVCQVLELESNESKPNDTEEDPVENVPCLQDGPESWDRLFKEQMQHIIHLWDLCHVSIIHRTQFYMLFRGDTSDQIYIEVEVRRLLWLQQHLDEVGEAPADDLAVSRASSMKALRNEREFLSRRMGSRMAEEERERLFIKWQVPLEAKQRKLQLVNMLWTEPGDEVHVEESADIVARLVGFCEGGNVSKEMFELNFAVPASRKPWLMGWQPISNMIREKAVLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1GPD6</accession>
    <name>I1GPD6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100834269</name>
        <name evidence="4" type="ORF">BRADI_1g11882v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561018.1">
        <property type="nucleotide sequence ID" value="XM_003560970.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GPD6"/>
    <dbReference type="STRING" id="15368.I1GPD6"/>
    <dbReference type="EnsemblPlants" id="KQK13695">
        <property type="protein sequence ID" value="KQK13695"/>
        <property type="gene ID" value="BRADI_1g11882v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834269"/>
    <dbReference type="Gramene" id="KQK13695">
        <property type="protein sequence ID" value="KQK13695"/>
        <property type="gene ID" value="BRADI_1g11882v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834269"/>
    <dbReference type="eggNOG" id="KOG1870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098841_2_2_1"/>
    <dbReference type="OMA" id="LHIENYS"/>
    <dbReference type="OrthoDB" id="1932324at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000005">
        <property type="entry name" value="50S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_uL18_bac/plant/anim"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899:SF19">
        <property type="entry name" value="OS03G0694800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK13695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="114" mass="12159" checksum="C7764DDEF416F23E" modified="2012-06-13" version="1">MVIPPPARAPAITNFLKPYVLKMHFTSNFVSAQVIHTPSATIACAASSQEKILRPSMESTRDVAAAAKIGKLLGERLLFRGIPAVSVSMSRDQTYHGKVKAVIDSLTAAGVKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1GQ07</accession>
    <name>I1GQ07_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100824851</name>
        <name evidence="12" type="ORF">BRADI_1g13740v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GQ07"/>
    <dbReference type="FunCoup" id="I1GQ07">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GQ07"/>
    <dbReference type="EnsemblPlants" id="KQK13981">
        <property type="protein sequence ID" value="KQK13981"/>
        <property type="gene ID" value="BRADI_1g13740v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13981">
        <property type="protein sequence ID" value="KQK13981"/>
        <property type="gene ID" value="BRADI_1g13740v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0498">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013069_3_0_1"/>
    <dbReference type="OMA" id="CHNDEME"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF98">
        <property type="entry name" value="OS03G0646300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="496"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK13981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="717" mass="82379" checksum="0A6B81D69DFF525C" modified="2012-06-13" version="1">MSSMSYPPASTGTTATRRSAFHIDYGASRRRLAMQPEALARGVITQGSAQLRTLGRSIRTGAAMAAVFQEDLKNTSRKIFDPQDRMLVRLNRSFLISCILSIAIDPLFFYTPRITDSMHGINICIGIDRELAVFTAVFRSVVDLFFVARIVLQFRTAFIAPSSRVFGRGELVIDTMEIAKRYFRRFFIADVLSIVPLPQLVVWLFTKRVRGTAVLATKDNLVLVILLQYVPRLARIYPLSTELKRTSGVFAETALAGAAYYLLWYMLASHIVGAFWYMLSIERLSDCWRNACDEFLGCNRIYMYCGRNLEEKEDPGFQEWITITRQVINETCEPQKDGEMPFNYGIYSSAVQSNVIGSLDVTSKILFCLWWGLANLSTLGQGLKTTIYTGEALFSITLATFGLILMAMLIGNIQTYLQSLTVRLEEMRVKRRDSEQWMHHRLLPQELRERVRRYDQYKWINTRGVDEEVLIQNLPKDLRRDIKRHLCLGLVRRVPLFANMDERLLDAICERLKPSLYTEKTHIIREGDPVDQMLFIIRGLLESITTDGGRSGFYNRSLLQEGAFCGEELLTWALDPKSGVSLPSSTRTVMALSEVESFALHAEELKFVAGQFRRMHSKQVQHTFRFYSQQWRTWAATYIQAAWRRHLKRKAAELRRREEEEEEGRSNSFKTTILVSRFAANALRGVHRQRSRKAEDGEIMIHVPVPKPREPDFGIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1GT36</accession>
    <name>I1GT36_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">BRADI_1g23730v3</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GT36"/>
    <dbReference type="FunCoup" id="I1GT36">
        <property type="interactions" value="187"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK15568">
        <property type="protein sequence ID" value="KQK15568"/>
        <property type="gene ID" value="BRADI_1g23730v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15568">
        <property type="protein sequence ID" value="KQK15568"/>
        <property type="gene ID" value="BRADI_1g23730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846686"/>
    <dbReference type="eggNOG" id="ENOG502QSJ4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_62_3_1"/>
    <dbReference type="OMA" id="TLSWHER"/>
    <dbReference type="OrthoDB" id="543442at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901001">
        <property type="term" value="P:negative regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000051">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase V.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000112">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000517">
        <property type="entry name" value="Putative receptor kinase Lecrk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019825">
        <property type="entry name" value="Lectin_legB_Mn/Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00307">
        <property type="entry name" value="LECTIN_LEGUME_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094088" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="21"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="348"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQK15568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="675" mass="74660" checksum="65DF025CAEB60F71" modified="2012-06-13" version="1">MLSLFLLLLLSLGGEMASSADDGQFTYQGFAAANLTLDGLATVLPNGLLALTNFTYQTKGHAFHPVPLRFLNKPTSTNTTAVARSFSTSFVFAVVSRYDGLSDQGLAFVVTPTMNLSTANAGQYLGLLNATNGTASNRILAVELDTIMNPEFSDINSNHVGINVNSLMSRQAKPAGYYGDDDGAFRGLMLNSRKLMQVWVDYDGQGRQLNVTLAPIQVPKPRKPLLSEVIDLSTFMEDPMYVGFSSSSGVVFTHHYVLGWSFSLDGPSPPLHFSKLPTLPRVGPKPRSKLLDVVLPLATALLVAAVLVVVFFFVWRRHRYAEVREDWEDEFGPHRFAYKDLFCATDGFKDKNLLGAGGFGKVYKGVLPASSLEVAVKRVSHGSRQGVREFVAELVSIGRLRHRNLAQLLGYCRRKSELLLVYDYMENGSLDKYLYNKNGPSLNWQERYWVIKGIASSLLYLHEDWEQIVIHRDIKASNVLLDSQMNARLGDFGLARLYDHGMDGQTTHVVGTMGYLAPELLRTGKATPATDIFAFGVFLLEVVCGRRPVESEKHGNKMVLIDWVLEHHRNGSILDTVDPGLMGKFDIQEVTIVLKLGLLCAHPSPNTRPSARKVMQYLDRSRSVPNLSPTYISYSTLALMQNEGFDSYIMPCPLFAKSVHTVSGDSSVTVLLDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1GT88</accession>
    <name>I1GT88_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome-recycling factor, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein OsL8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ribosome-releasing factor, chloroplastic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100834081</name>
        <name evidence="14" type="ORF">BRADI_1g24110v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for the release of ribosomes from messenger RNA at the termination of chloroplastic protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRF family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562889.1">
        <property type="nucleotide sequence ID" value="XM_003562841.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010236683.1">
        <property type="nucleotide sequence ID" value="XM_010238381.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GT88"/>
    <dbReference type="STRING" id="15368.I1GT88"/>
    <dbReference type="EnsemblPlants" id="KQK15638">
        <property type="protein sequence ID" value="KQK15638"/>
        <property type="gene ID" value="BRADI_1g24110v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK15639">
        <property type="protein sequence ID" value="KQK15639"/>
        <property type="gene ID" value="BRADI_1g24110v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74897">
        <property type="protein sequence ID" value="PNT74897"/>
        <property type="gene ID" value="BRADI_1g24110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834081"/>
    <dbReference type="Gramene" id="KQK15638">
        <property type="protein sequence ID" value="KQK15638"/>
        <property type="gene ID" value="BRADI_1g24110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK15639">
        <property type="protein sequence ID" value="KQK15639"/>
        <property type="gene ID" value="BRADI_1g24110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74897">
        <property type="protein sequence ID" value="PNT74897"/>
        <property type="gene ID" value="BRADI_1g24110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834081"/>
    <dbReference type="eggNOG" id="KOG4759">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073981_3_0_1"/>
    <dbReference type="OMA" id="FNPMNNG"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032544">
        <property type="term" value="P:plastid translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00520">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000001">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.20:FF:000017">
        <property type="entry name" value="Ribosome-recycling factor chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00040">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00496">
        <property type="entry name" value="frr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982:SF3">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME RECYCLING FACTOR PSEUDO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="RIBOSOME RECYCLING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="Ribosome recycling factor, RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="9 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ribosome recycling factor" evidence="13">
        <location>
            <begin position="102"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="213"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK15638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="266" mass="29300" checksum="B9799075F52E418D" modified="2012-06-13" version="1">MALHAVSPAAAASPPCALSSARLPQRPGCGCLSSRSSLLSSSTNFMKLHSGAVGFFSAPLVIQYSDKRAVLRHATIEEIEAEKTIIEDQAKEKMEKAIETVQTNFNTVRTGRANPAMLDRIEVEYYGTPVNLKSIAQINTPDATSLLIQPYDKTCLKLIEKAIVAANIGVTPSNDGEVIRVTVPPLTSDRRKEMTKTVAKLSEEGKVAIRNIRRDAIKAYDKLEKEKKLSEDNVKDLSADLQKLTDEYMKKVEAIQKQKEQELSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTH9</accession>
    <name>I1GTH9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100836529</name>
        <name evidence="4" type="ORF">BRADI_1g24850v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562897.1">
        <property type="nucleotide sequence ID" value="XM_003562849.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GTH9"/>
    <dbReference type="STRING" id="15368.I1GTH9"/>
    <dbReference type="EnsemblPlants" id="KQK15777">
        <property type="protein sequence ID" value="KQK15777"/>
        <property type="gene ID" value="BRADI_1g24850v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836529"/>
    <dbReference type="Gramene" id="KQK15777">
        <property type="protein sequence ID" value="KQK15777"/>
        <property type="gene ID" value="BRADI_1g24850v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836529"/>
    <dbReference type="eggNOG" id="KOG0439">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067947_0_0_1"/>
    <dbReference type="OMA" id="LPWWQKK"/>
    <dbReference type="OrthoDB" id="845153at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GTH9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF113">
        <property type="entry name" value="OS07G0558800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MSP" evidence="3">
        <location>
            <begin position="80"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK15777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="267" mass="29348" checksum="2CA29172D2C7DDA5" modified="2012-06-13" version="1">MAISGDDRYAPAGDSGSVKLWNLCRMPFRQAGGAPASSSGIHQSASRYGHEAPVAADGSAPGASAGISTMAKALLPARRRLRLDPPNKLYFPYEPGKQVKSAIRIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETVIATVFKFVEHPENNENVLQKCKVKFKILSLKVKEPMDYAPELFDEQKDQAVVEKLLRVIFLDVQNPGPQLEKLNTQLAEAEAALEARKKPPEESGPRIVGEGLVIDEWKERRERYLAQQQVEVVDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTU2</accession>
    <name>I1GTU2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein GOS2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein eIF1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Translation initiation factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100840498</name>
        <name evidence="11" type="ORF">BRADI_1g25837v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in translation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562997.1">
        <property type="nucleotide sequence ID" value="XM_003562949.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GTU2"/>
    <dbReference type="STRING" id="15368.I1GTU2"/>
    <dbReference type="EnsemblPlants" id="KQK15926">
        <property type="protein sequence ID" value="KQK15926"/>
        <property type="gene ID" value="BRADI_1g25837v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840498"/>
    <dbReference type="Gramene" id="KQK15926">
        <property type="protein sequence ID" value="KQK15926"/>
        <property type="gene ID" value="BRADI_1g25837v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840498"/>
    <dbReference type="eggNOG" id="KOG1770">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082805_3_0_1"/>
    <dbReference type="OMA" id="VENHIHI"/>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004499">
        <property type="entry name" value="SUI1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="10">
        <location>
            <begin position="33"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK15926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="115" mass="12703" checksum="09F5B3382E3F332C" modified="2012-06-13" version="1">MSDLDIQIPTAFDPFAEANAGEAGAAAGSKDYVHVRIQQRNGRKSLTTVQGLKKEFSYSKILKDLKKEFCCNGTVVQDPELGQVIQLQGDQRKNVSNFLVQAGIVKKEHIKIHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1GYN1</accession>
    <name>I1GYN1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein DETOXIFICATION</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_1g42410v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GYN1"/>
    <dbReference type="SMR" id="I1GYN1"/>
    <dbReference type="EnsemblPlants" id="PNT75964">
        <property type="protein sequence ID" value="PNT75964"/>
        <property type="gene ID" value="BRADI_1g42410v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75964">
        <property type="protein sequence ID" value="PNT75964"/>
        <property type="gene ID" value="BRADI_1g42410v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_1_4_1"/>
    <dbReference type="InParanoid" id="I1GYN1"/>
    <dbReference type="OMA" id="IWANTEQ"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT75964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="43255" checksum="D964D41C8C72ECFB" modified="2012-06-13" version="1">MASALDTLCGQAFGARQYHLLGMYKQRAMLLLTLVSIPLAAVWYYTGEILLLVGQDKDIAVEAGTYARWMIPELFAYGLLQCHACFLQTQNIVLPVMLSAAATALCHLAVCWLLVFALGLGSKGAAIGNAISYWINVAILAVYVRVSSSCKKTWTGFSMEAFHDPLSFFKLTVPSALMVCLEWWSFELLVLLSGFLPNPRLETSVLSITLNTSNCVFMIPFGLGAAISTRVSNELGAGRPQAARLAVQVVVFFALSEGLIMGVILICLRYVWGHAYSADEEVVTYIAKMMLVLAVSNFLDGIQCVLSGVARGCGWQKICAWINLGAFYAVGVPAAYLVAFVLRFGGMGLWMGIILGTVVQVLLLVTITLCMDWRKEATKAENRIFISSLPKDLLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZ14</accession>
    <name>I1GZ14_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100828174</name>
        <name evidence="15" type="ORF">BRADI_1g43560v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563952.1">
        <property type="nucleotide sequence ID" value="XM_003563904.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZ14"/>
    <dbReference type="FunCoup" id="I1GZ14">
        <property type="interactions" value="1630"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GZ14"/>
    <dbReference type="EnsemblPlants" id="KQK18597">
        <property type="protein sequence ID" value="KQK18597"/>
        <property type="gene ID" value="BRADI_1g43560v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828174"/>
    <dbReference type="Gramene" id="KQK18597">
        <property type="protein sequence ID" value="KQK18597"/>
        <property type="gene ID" value="BRADI_1g43560v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828174"/>
    <dbReference type="eggNOG" id="ENOG502QRT0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018420_0_0_1"/>
    <dbReference type="OMA" id="NISYYTQ"/>
    <dbReference type="OrthoDB" id="1874781at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11350:FF:000011">
        <property type="entry name" value="O-fucosyltransferase 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741:SF3">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741">
        <property type="entry name" value="OS02G0726500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK18597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="504" mass="57464" checksum="FEE92C2BAC5B8398" modified="2012-06-13" version="1">MGWKATMGGKAAGIGGEKHKCPPSSAAAARSRMKLWMVRATTTVLLWTCVVQLTAVGETWGPRVLKGWPSCLTASEEAAALAAVRPEPIVEKPALPPKRMYRNNGYLMVSCNGGLNQMRAAICDMVVIARYLNVTLVVPELDKTSFWNDPSEFQDIFDVEHFITSLRDEVRILRELPPRVKRRVELGMFHSMPPISWSDISYYHNQILPLIRKYKVLHLNRTDARLANNGLPMEIQKLRCRVNYGSLRFTAEIEDLGKRVIRMLRQNGPFLVLHLRYEMDMLAFSGCTQGCSNEEAEELTRMRYAYLWWKEKIINSDLKRKDGLCPLTPEETALVLRALDIDRSMQIYIAAGEIYGGKRRMAALTSAYPNVVRKETLLEPSDLMFFQNHSSQMAALDYMVSLESDIFVPTYDGNMAKVVEGHRRFMGFKKTILLDRKLIVDLVDQYNNGSLRWDEFSLLIKAAHAGRMGSASKRTVFPDRPKEEDYFYANPQECLHDPDLLHTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZI3</accession>
    <name>I1GZI3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100833537</name>
        <name evidence="9" type="ORF">BRADI_1g45060v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010227818.1">
        <property type="nucleotide sequence ID" value="XM_010229516.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZI3"/>
    <dbReference type="FunCoup" id="I1GZI3">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK18839">
        <property type="protein sequence ID" value="KQK18839"/>
        <property type="gene ID" value="BRADI_1g45060v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833537"/>
    <dbReference type="Gramene" id="KQK18839">
        <property type="protein sequence ID" value="KQK18839"/>
        <property type="gene ID" value="BRADI_1g45060v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013137_2_0_1"/>
    <dbReference type="OMA" id="CVDRWFM"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GZI3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46151">
        <property type="entry name" value="NEP1-INTERACTING PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46151:SF6">
        <property type="entry name" value="OS06G0231600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="185"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK18839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="230" mass="24303" checksum="5D864DCE968E4D30" modified="2012-06-13" version="1">MEVASAEGVSPRSPGAASEEEHRAATCLPRLVAGVLSGVLTGLFAVAGGLTGAITGALAGRASDSGVLRGAGLGAFAGAVLSIEVLEASRAYWCADRSSPQSTSSMGDFVDQLIHARFVQEQYEPSVYMAYRWQQVGVAEFGNDDLHGILGEASSYGLSQDSLKKLPCHVVTDQKQEPVDENLSCPICLQDIVTGETARRLPKCSHSFHQPCVDKWLIGHGSCPVCRQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>I1H664</accession>
    <name>I1H664_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phytochrome B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100829838</name>
        <name evidence="17" type="ORF">BRADI_1g64360v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses. Pfr controls the expression of a number of nuclear genes including those encoding the small subunit of ribulose-bisphosphate carboxylase, chlorophyll A/B binding protein, protochlorophyllide reductase, rRNA, etc. It also controls the expression of its own gene(s) in a negative feedback fashion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="12">Contains one covalently linked phytochromobilin chromophore.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phytochrome family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558068.1">
        <property type="nucleotide sequence ID" value="XM_003558020.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H664"/>
    <dbReference type="SMR" id="I1H664"/>
    <dbReference type="FunCoup" id="I1H664">
        <property type="interactions" value="508"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H664"/>
    <dbReference type="EnsemblPlants" id="KQK21982">
        <property type="protein sequence ID" value="KQK21982"/>
        <property type="gene ID" value="BRADI_1g64360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829838"/>
    <dbReference type="Gramene" id="KQK21982">
        <property type="protein sequence ID" value="KQK21982"/>
        <property type="gene ID" value="BRADI_1g64360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829838"/>
    <dbReference type="eggNOG" id="ENOG502QRNS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010418_0_0_1"/>
    <dbReference type="OMA" id="DDNTCCS"/>
    <dbReference type="OrthoDB" id="2015534at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H664">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009584">
        <property type="term" value="P:detection of visible light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017007">
        <property type="term" value="P:protein-bilin linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009585">
        <property type="term" value="P:red, far-red light phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16932">
        <property type="entry name" value="HATPase_Phy-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000029">
        <property type="entry name" value="Phytochrome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000034">
        <property type="entry name" value="Phytochrome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000039">
        <property type="entry name" value="Phytochrome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.270:FF:000001">
        <property type="entry name" value="Phytochrome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000044">
        <property type="entry name" value="Phytochrome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013654">
        <property type="entry name" value="PAS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044767">
        <property type="entry name" value="Phy_HATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016132">
        <property type="entry name" value="Phyto_chromo_attachment"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013516">
        <property type="entry name" value="Phyto_chromo_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001294">
        <property type="entry name" value="Phytochrome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012129">
        <property type="entry name" value="Phytochrome_A-E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013515">
        <property type="entry name" value="Phytochrome_cen-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043150">
        <property type="entry name" value="Phytochrome_PHY_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47876">
        <property type="entry name" value="OS08G0260000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47876:SF3">
        <property type="entry name" value="PHYTOCHROME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08446">
        <property type="entry name" value="PAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00360">
        <property type="entry name" value="PHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000084">
        <property type="entry name" value="Phytochrome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01033">
        <property type="entry name" value="PHYTOCHROME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00245">
        <property type="entry name" value="PHYTOCHROME_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50046">
        <property type="entry name" value="PHYTOCHROME_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Phytochrome chromophore attachment site" evidence="14">
        <location>
            <begin position="269"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="16">
        <location>
            <begin position="671"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="16">
        <location>
            <begin position="805"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="15">
        <location>
            <begin position="953"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="12">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>phytochromobilin</name>
            <dbReference type="ChEBI" id="CHEBI:189064"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000084-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK21982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1181" mass="129278" checksum="338E885384337D0F" modified="2012-06-13" version="1">MASGSRATPTRSPSSARPAASRQAEQQHHYTQSSGGSTSRAGGGGGGGGGEGGGGGGAAASAAATESVSKAVAQYTLDAGLHAVFEQSGASGRSFDYSQSLLAPPSTSSEQQIAAYLSRIQRGGHIQPFGCTLAVADDSSFRLLAFSENAADLLDLSPHHSVPSLDSSAAPPPVSLGADSRLLFSPPSGVLLERAFAAREISLLNPLWIHSRVSSKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVRAISRLQALPGGDVKLLCDTVVEHVRELTGYDRVMVYKFHDDEHGEVLAESRRTDLEPYLGLHYPATDIPQASRFLFRQNRVRMIADCHAAPVRVIQDPAMPQPLCLVGSTLRSPHGCHAQYMANMGSIASLVMAVIISSGGEDEHNMGRGAIPSAMKLWGLVVCHHTSPRCIPFPLRYACEFLMQAFGLQLNMELQLAYQLSEKHILRTQTLLCDMLLRDSPTGIVTQSPSIMDLVKCDGAALYYHGKYYPLGVTPTEVQIKDIIEWLTVCHGDSTGLSTDSLADAGYSGATALGDAVCGMAVAYITPSDYLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFKAFLEVVKSRSLPWENAEMDAIHSLQLILRDSFRDAREGTSNSKAIVDGQVQLGELELRGIDELSSVAREMVRLIETATVPIFAVDTDGCINGWNAKVAELTGLTVEEAMGKSLVTDLIFKESEEIVEKLLSQALRGEEDKNVEIKLKTFGPEQSKGAIFVIVNACSSRDYTKNIVGVCFVGQDITGQKVVMDKFVNIQGDYKAIVHNPNPLIPPIFASDENICCSEWNTAMEKLTGWSRGEVIGKLLVGEVFGNCCRLKGPDALTKFMIALHNAIGGQDSEKLPFSFFDKNGKYVQALLTANTRSKMDGEAIGAFCFLQIASPELQQAFEIQRQQEKKCYARMKELAYICQEIKNPLSGIRFTNSLLEMTDLKDDQRQFLETSTACEKQMSKIVKDASLQRIEDGSLVLEKGEFSLGNVMNAVVSQVMILLRERDLQLIRDIPDEIKEASAYGDQYRIQQVLSDFLLSMVRFAPTENGWVEIQVRPNVKQNSDGTETMLFLFRFACPGEGLPPDIVQDMFSNSRWTTQEGIGLSVCRKILKLMGGEVQYIRESERSFFLIVLELPQPLQAESRERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6X0</accession>
    <name>I1H6X0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100826172</name>
        <name evidence="2" type="ORF">BRADI_1g66460v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558224.1">
        <property type="nucleotide sequence ID" value="XM_003558176.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6X0"/>
    <dbReference type="EnsemblPlants" id="KQK22318">
        <property type="protein sequence ID" value="KQK22318"/>
        <property type="gene ID" value="BRADI_1g66460v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826172"/>
    <dbReference type="Gramene" id="KQK22318">
        <property type="protein sequence ID" value="KQK22318"/>
        <property type="gene ID" value="BRADI_1g66460v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826172"/>
    <dbReference type="eggNOG" id="ENOG502RZNE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_18_1_1"/>
    <dbReference type="OMA" id="CFAYPDV"/>
    <dbReference type="OrthoDB" id="10066542at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050383">
        <property type="entry name" value="GlyoxalaseI/FosfomycinResist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366">
        <property type="entry name" value="GLYOXALASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366:SF22">
        <property type="entry name" value="VOC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="9"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="138" mass="15185" checksum="50DF7826E289AF0C" modified="2012-06-13" version="1">MASGAVRPALAYIVVYVEDVAKSAACYAAAFGYSVRRIDQSHKWAELDTGSTTIAFTPLHQRETDALTGEVQLPKSPRERGPVEICFDYTDVDAAYRRAVDNGAVPVTPPEQKNWGQKVGYVRDLDGNIVRMGSHVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1H7C7</accession>
    <name>I1H7C7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100842717</name>
        <name evidence="5" type="ORF">BRADI_1g67820v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561812.1">
        <property type="nucleotide sequence ID" value="XM_003561764.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H7C7"/>
    <dbReference type="EnsemblPlants" id="KQK22523">
        <property type="protein sequence ID" value="KQK22523"/>
        <property type="gene ID" value="BRADI_1g67820v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842717"/>
    <dbReference type="Gramene" id="KQK22523">
        <property type="protein sequence ID" value="KQK22523"/>
        <property type="gene ID" value="BRADI_1g67820v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842717"/>
    <dbReference type="eggNOG" id="KOG1584">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027239_0_1_1"/>
    <dbReference type="OMA" id="MTHITID"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="85"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK22523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="350" mass="38807" checksum="DF4148C7A11EB2E6" modified="2012-06-13" version="1">MVSNATSTQAGPVPFKDFEDDVHQATVAPVPERPPEDEDVLVSSLPSTVLPGVPFFKLRFYQGFWLHEGLIPGAIALQRRFEPHPDDVVVASIPKCGTTWLAALAFATMARRAYPPAGADHPLLRLNPHQVAPFMDMFFAQGRDAVLDALPSPRLMFTHMPLAMLPRAVPPASGAGGGGCRVVYICREPKDMAVSLWHFRRALCPELSFADTFESLCAGHSSGPFWDHILGYWRASAATPDNVLFLRYEELLRDPAENVRKLARFVGLPFSDAEEETGVVHAVVELCNLDNMRSLETNKSGHLPYVNFPIKSLFRKGVAGDWVNHMTPEMARRMDDIVAEKFRDTGLTFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1H899</accession>
    <name>I1H899_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cp protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100842174</name>
        <name evidence="1" type="ORF">BRADI_1g70440v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558523.1">
        <property type="nucleotide sequence ID" value="XM_003558475.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H899"/>
    <dbReference type="FunCoup" id="I1H899">
        <property type="interactions" value="702"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H899"/>
    <dbReference type="EnsemblPlants" id="KQK22979">
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22979"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19421" checksum="C843BEBEA039EDFC" modified="2012-06-13" version="1">MEKALTKLSSFTIPRKAKQELSAIGGDISRLSSTVEEKAKWVFDKLKGKPNKSLSDLLREYNLPPGLFPQNIICYEYDQTSSKLVVHLAKPCEVSFKDSSMIRFAPRVKATLSRGKLSGIEGMKTKVVVWVKVTSVNLESFRSDKVCFIAGVKKLRQKDAYEMPREAISVGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I1H8W4</accession>
    <name>I1H8W4_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g72260v3</name>
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    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H8W4"/>
    <dbReference type="EnsemblPlants" id="KQK23254">
        <property type="protein sequence ID" value="KQK23254"/>
        <property type="gene ID" value="BRADI_1g72260v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g72260v3"/>
    </dbReference>
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    <dbReference type="OMA" id="QINNSVW"/>
    <dbReference type="OrthoDB" id="719620at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47865">
        <property type="entry name" value="OS05G0580550 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47865:SF1">
        <property type="entry name" value="OS08G0106700 PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        </location>
    </feature>
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        <location>
            <begin position="8"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK23254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="142" mass="15525" checksum="EE38E37CF9E47F1B" modified="2012-06-13" version="1">MAGVVVESTKTIGTATTSTESDEDEDCISTARSSAKRPAKKAKKDVSEEEDLISTLGRVGSELAAAIVTAAKKSAPQPPPVDEIPDNLYETLLGMEGYSETQIDDYYIFLVSNPRSAKAFMKMGHVGQLVWMDRYINKEFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9G2</accession>
    <name>I1H9G2_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
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    </gene>
    <organism evidence="3">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312862.1">
        <property type="nucleotide sequence ID" value="XM_024457094.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312863.1">
        <property type="nucleotide sequence ID" value="XM_024457095.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_024457096.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312866.1">
        <property type="nucleotide sequence ID" value="XM_024457098.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312867.1">
        <property type="nucleotide sequence ID" value="XM_024457099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H9G2"/>
    <dbReference type="STRING" id="15368.I1H9G2"/>
    <dbReference type="EnsemblPlants" id="KQK23518">
        <property type="protein sequence ID" value="KQK23518"/>
        <property type="gene ID" value="BRADI_1g74330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581871"/>
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        <property type="gene ID" value="BRADI_1g74330v3"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008004">
        <property type="entry name" value="OCTOPUS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31659:SF4">
        <property type="entry name" value="OS11G0707900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31659">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <location>
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            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="259"/>
        </location>
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            <end position="259"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK23518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="488" mass="53445" checksum="95C2ACD7D0AFECF8" modified="2012-06-13" version="1">MDQPPPVPSICGLHPGIAVTGFCSACLRERLAGLHPADPAELRRCKSFSYARSAAAYFEPQRRSCDARGAAIFHHQDLPPGHGEDELEDVPPTSTVRPMKDHISQDSSKKTTFGGGLGKKWQEWRRKSKLKKQGPAAPAVATAAASRAAIDAHRSFRDTHSEVAIGRRSVDVDSSRLWMDAGRISVDEPPRASWQRLPPTVEDAPIPRSDGQIPVEEEDDDAEPGGCAQTRDYYLDSSSSSRRRRSVDRSSFSSRKSFSDTNDLPRVIAAANANARVSPAIGAEFYHYHHHAQGQSVLDHNQHWELHGPNSYSLRDDDMSGSFNSAAFQEGVPVPLPAKKSNKWIKNIWGLIHKKSSTKESQAASIANRSFSETWPELRARGYNGQMLRCNSSVSARSSFSNSGAAVGAVNGRRRSNAEMHVNGLGRARKDEVLLERNFSARYSTCPVDNGVFLNPVGGSRRHQNGMSGKGRPARSSNSLPRSALGMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9V3</accession>
    <name>I1H9V3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100834139</name>
        <name evidence="14" type="ORF">BRADI_1g75530v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="3"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558833.1">
        <property type="nucleotide sequence ID" value="XM_003558785.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229227.1">
        <property type="nucleotide sequence ID" value="XM_010230925.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229228.1">
        <property type="nucleotide sequence ID" value="XM_010230926.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H9V3"/>
    <dbReference type="FunCoup" id="I1H9V3">
        <property type="interactions" value="1197"/>
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        <property type="gene ID" value="BRADI_1g75530v3"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="entry name" value="CYSTEINE--TRNA LIGASE 1, CYTOPLASMIC-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
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    </dbReference>
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        <property type="entry name" value="tRNA-synt_1e"/>
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    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
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        <location>
            <begin position="63"/>
            <end position="357"/>
        </location>
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        <location>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="562" mass="64228" checksum="EC44B98127B8150C" modified="2012-06-13" version="1">MAENSSAPAGATLLSNLTMADDSTSPATTTLPSVPTTGEDKPKPSPPQLHLFNSMTKKKEPFVPRVEGKVGMYVCGVTPYDFSHIGHARAYVAFDVLYRYLKFLGYEVEYVRNFTDIDDKIIDRANKAGETVTSLSTRFINEFLIDMAKLQCLPPTCEPRVTEHIEHIIDLIAEIIKNDKAYVDGDVFFSIDSFPEYNSLSGRNLDDNRPGSRVAVDPRKRNRNDFALWKAAKEGEPFWESPWGRGRPGWHIECSAMSAHYLGHVFDIHGGGKDLIFPHHENELAQSRAAYPDSEVKCWMHNGFVNKDDEKMSKSLNNFFSIREIITLYHPLALRFFLMRTHYKSDVNHSDKALEIASDRVYYIYQTLHDCDEVLSLYREENISVPVPDEEQKLVDEHHTAFLDSMSDDLRTTDVLDGFMGLLKAINSNLNDFKKLQQKLEQQKKKQQQKKQQQKQQRPQKQPEDHIQALISLEAEIKNKLSILGLMPPSSLVEVLKELKEKALKRAGLTEETLREQIEQRVAARNNKQFDVSDQIRKQLASKGIALMDEPTGTVWRPCERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAI0</accession>
    <name>I1HAI0_BRADI</name>
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        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="primary">LOC100823104</name>
        <name evidence="2" type="ORF">BRADI_1g77420v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003558920.4"/>
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        <property type="gene ID" value="BRADI_1g77420v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823104"/>
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        <property type="gene ID" value="BRADI_1g77420v3"/>
    </dbReference>
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        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
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    </dbReference>
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        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
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            <begin position="75"/>
            <end position="95"/>
        </location>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="42339" checksum="825439630962DDA1" modified="2012-06-13" version="1">MSDVGCGWDFLDWVGPDLAASAFGLLDHPADLVRAAAVSRSWRRCAVESGVCKSLCVKLCPEAAVFTSAVEVSRSPPTAAESSSGGGSGGREDDARSQESQFRIFSCLCGAVLHSTTKPDADCIQNCFGASSTDNFPDESMDSTLQPQDRINLNPSYWSSGGQDDPDVPESLTYKLASDICVVREIKLQPFKAYFQIGFPIYSPKMVRFRFGHCKLPHGTESYLTDEDENLAMIADENYVWTYTSPEFPVLQENMLQSFRLPRPVLCIGGVVKIELLGRVQKQATDDKYYICVCHAQVVGRSLSPVFVVDIADAGGHAVLKYSPGVRNLGAEDVKQEDARDSTDWHSLVARYRQVRHLAIMNVLLGPPQSMEEDDAGVVSDDDPFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAW0</accession>
    <name>I1HAW0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formyl transferase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100844926</name>
        <name evidence="4" type="ORF">BRADI_1g78480v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313214.1">
        <property type="nucleotide sequence ID" value="XM_024457446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAW0"/>
    <dbReference type="FunCoup" id="I1HAW0">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HAW0"/>
    <dbReference type="EnsemblPlants" id="KQK24163">
        <property type="protein sequence ID" value="KQK24163"/>
        <property type="gene ID" value="BRADI_1g78480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844926"/>
    <dbReference type="Gramene" id="KQK24163">
        <property type="protein sequence ID" value="KQK24163"/>
        <property type="gene ID" value="BRADI_1g78480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844926"/>
    <dbReference type="eggNOG" id="KOG3076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_3_1_1"/>
    <dbReference type="OMA" id="QILNIHH"/>
    <dbReference type="OrthoDB" id="4239773at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008864">
        <property type="term" value="F:formyltetrahydrofolate deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:one-carbon metabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ACT_F4HF-DF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FMT_core_Formyl-FH4-Hydrolase_C"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Formyl-FH4-Hydrolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
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        <property type="entry name" value="PurU"/>
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        <property type="entry name" value="PurU_ACT"/>
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    <dbReference type="NCBIfam" id="NF004684">
        <property type="entry name" value="PRK06027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706">
        <property type="entry name" value="FORMYLTETRAHYDROFOLATE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706:SF1">
        <property type="entry name" value="FORMYLTETRAHYDROFOLATE DEFORMYLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036480">
        <property type="entry name" value="FormyFH4_hydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01575">
        <property type="entry name" value="FFH4HYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="3">
        <location>
            <begin position="107"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK24163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="303" mass="34279" checksum="44687207F5694553" modified="2012-06-13" version="1">MLSLARRPLSAAVPVGNLLGIHLFQCPDAVGIVAKLSECIATRGGNIHSVDVFVPDDKPVFYARSEFTYNPRLWPRDELGADFLHLSNHFKAQKSTVRVPDLDPKYKISVLASKQDHCLFDLLHRWQEGRLPVDINCVISNHDRPTDNHVRRFLQRHGIPYHYLPTTPGNKREQEILELIEGTDFVVLARYMQVMSESFLKAYGKDIINIHHGLLPSFKGGSPSRQAFNAGVKLIGATSHFVTPELDAGPIIEQMVERVSHRDTLQSFVVKSENLEKQCLAEAIKSYCELRVLPYELMKTVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HBZ6</accession>
    <name>I1HBZ6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100837075</name>
        <name evidence="4" type="ORF">BRADI_2g03120v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568629.1">
        <property type="nucleotide sequence ID" value="XM_003568581.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HBZ6"/>
    <dbReference type="STRING" id="15368.I1HBZ6"/>
    <dbReference type="EnsemblPlants" id="KQK02693">
        <property type="protein sequence ID" value="KQK02693"/>
        <property type="gene ID" value="BRADI_2g03120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837075"/>
    <dbReference type="Gramene" id="KQK02693">
        <property type="protein sequence ID" value="KQK02693"/>
        <property type="gene ID" value="BRADI_2g03120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837075"/>
    <dbReference type="eggNOG" id="KOG1793">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040584_2_1_1"/>
    <dbReference type="OMA" id="REMKEMW"/>
    <dbReference type="OrthoDB" id="21124at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HBZ6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044204">
        <property type="entry name" value="IWS1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47350">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47350:SF4">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="3">
        <location>
            <begin position="306"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK02693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="516" mass="58077" checksum="C1945E44C2B52647" modified="2012-06-13" version="1">MDDFFDEDGEQLMDPDARDLSPEPQAQPYEDLEDDLGDEDGAGAGWNRDRSPTPVHGDDGAGSSSRPRKRLLKKGGGGVPGDDLEDWGEEAAGLADEGLEDHDADAARKRKGSSSLRDLARGGGKEKHEKKRRKDDRVVERERRGLSSGGKGGGADEQEEGEREIQELWDTIAGAGSEDDQEGVRTADDDNFIDDTGVDPADRYDNDNGGHSPRHYAQAEEAEEDDEIERLFKGGKKKKKNDRPRADIGLIVEQFIAEFEVASEEDANLNRQSKPAINKLMKLPLLIEVLSKKNLQQEFLDHGILTLLKNWLEPLPDGSMPNMNIRTAVLKLLSDFPIDLEQFDRREQLKKSGLGKVIMFLSKSDEETTSNRKLAKELVDKWSRPIFQKSTRFEDMRRYDDERAPYRRPQMKKPSSSSSGMESRDDDLDADFSQRKSGQSGSRQHASRPEASPLDFVIRPQSKIDPEQIRARAKQVVQDQRRLKMNKKLQQLKAPKKKNLQASKLSVEGRGMVKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1HDK8</accession>
    <name>I1HDK8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100826432</name>
        <name evidence="7" type="ORF">BRADI_2g07860v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566029.1">
        <property type="nucleotide sequence ID" value="XM_003565981.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HDK8"/>
    <dbReference type="FunCoup" id="I1HDK8">
        <property type="interactions" value="1058"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HDK8"/>
    <dbReference type="EnsemblPlants" id="KQK03436">
        <property type="protein sequence ID" value="KQK03436"/>
        <property type="gene ID" value="BRADI_2g07860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826432"/>
    <dbReference type="Gramene" id="KQK03436">
        <property type="protein sequence ID" value="KQK03436"/>
        <property type="gene ID" value="BRADI_2g07860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826432"/>
    <dbReference type="eggNOG" id="ENOG502QV38">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_429860_0_0_1"/>
    <dbReference type="OMA" id="TRMEMSN"/>
    <dbReference type="OrthoDB" id="118154at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HDK8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03572">
        <property type="entry name" value="ENTH_like_Tepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035802">
        <property type="entry name" value="ENTH/VHS_tepsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039273">
        <property type="entry name" value="TEPSIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514:SF1">
        <property type="entry name" value="OS01G0232400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VHS" evidence="6">
        <location>
            <begin position="35"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="194"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="404"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK03436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="689" mass="74695" checksum="033EBDF984DFD9C5" modified="2012-06-13" version="1">MDQSRRAVESYWRSRMVDGVTAEDDKVAPVYKLEEISELLRASDASIVKEVADFVLKRLDNKSPVVKQKALRLIKYAVGKSGTDFKQEMQQHSAAMRQLIHYKGHPDPLKGDALNKAVRETANKAIAAVFSTEDPKAVVVTDGLGKRIQGFGNTNFEPSRDDKKSFLSELTEVVGIGGASIKQELSNFAATHSMMTNDNGGMYKNPIRRSLTTETDRYGRYDPSEIQGETRATSGASKNVSSGSWGPTPSSSASTDDTGSSQPGIKTREERLLETIVTASGVRLQPTRDALQIFLTEASKLDAVALSHALDNKLNSPLWQVRMKAICVLEAIVRKQDIDPYSIVASYFSENSASIVRCCELPQVSLREKASKVLNLLVGEQPSGTKDLSQTKTALPAAVQMPDLIDTGDQDDPGTQSSAQESSEQTMGNSTYVSLVDDLLGGEPIGNISSTSNGNGGDPFADVSFHEEEIKETNDLFSGMTVEEKSSASMQDNTPMDTNGLPDIFGGNPEPFFQDRVDDKGTVNDLMAGLNLNGTVQAQPGVKAESNSNLNGSQFFDMNNQTSHMVSPAALNGILGQSSFYQQVPLQYNLPQLMFNQSFPGQQLNYGAMEALLAQQQQLLQNLGNFNAGPGHSCFNTINNGNTSVMPDIFNSSNQPQHPVAVMSNSKKEETKAFDFVSDHLAAARGSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1HID7</accession>
    <name>I1HID7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g22187v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HID7"/>
    <dbReference type="STRING" id="15368.I1HID7"/>
    <dbReference type="EnsemblPlants" id="KQK05732">
        <property type="protein sequence ID" value="KQK05732"/>
        <property type="gene ID" value="BRADI_2g22187v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05732">
        <property type="protein sequence ID" value="KQK05732"/>
        <property type="gene ID" value="BRADI_2g22187v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_16_4_1"/>
    <dbReference type="InParanoid" id="I1HID7"/>
    <dbReference type="OMA" id="NDLFKWN"/>
    <dbReference type="OrthoDB" id="1839843at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK05732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="67" mass="7393" checksum="49362EE77AC9E19D" modified="2012-06-13" version="1">MASKRILKELKDLQKDPPTSCSAGPVGEDMFHWQAPIMGPPDSPYAGGVFLVNIHFPPDYPFKPPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1HL14</accession>
    <name>I1HL14_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100840303</name>
        <name evidence="8" type="ORF">BRADI_2g33100v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231616.1">
        <property type="nucleotide sequence ID" value="XM_010233314.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HL14"/>
    <dbReference type="STRING" id="15368.I1HL14"/>
    <dbReference type="EnsemblPlants" id="KQK07106">
        <property type="protein sequence ID" value="KQK07106"/>
        <property type="gene ID" value="BRADI_2g33100v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840303"/>
    <dbReference type="Gramene" id="KQK07106">
        <property type="protein sequence ID" value="KQK07106"/>
        <property type="gene ID" value="BRADI_2g33100v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840303"/>
    <dbReference type="eggNOG" id="ENOG502QPUX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051752_4_0_1"/>
    <dbReference type="OMA" id="PFIHGTK"/>
    <dbReference type="OrthoDB" id="777167at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF2">
        <property type="entry name" value="OS05G0199100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="7">
        <location>
            <begin position="125"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK07106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="293" mass="30082" checksum="A76769D2545E3034" modified="2012-06-13" version="1">MAERVYPAAKPNPPPAMANGAGAGAGAPAAAAPKPQMYQRPIYRPQGAGKSRRGRSCRCSFCCCFCWALLVIILLAIVAAAAGGAFYLLYRPQRPSFSVSSVRLTAFNLSSSATAPVLTDSISLTVTAKNPNKKLVYFYDDFSFSAATAANAVPLGESASPGFAHAAGNTTVFTATVAAAALTVDPSGASSDLKKSGAFSVAIDAETRAGVKVGGLKTKKIGIQVRCEGIKVTPPSPPPPAPKKTKGKNGTALAAPAPAPALDTAEPSTTATVSTAAHACKVRVRVKIWKWTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HR99</accession>
    <name>I1HR99_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100839809</name>
        <name evidence="5" type="ORF">BRADI_2g49067v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232274.1">
        <property type="nucleotide sequence ID" value="XM_010233972.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HR99"/>
    <dbReference type="EnsemblPlants" id="KQK09608">
        <property type="protein sequence ID" value="KQK09608"/>
        <property type="gene ID" value="BRADI_2g49067v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72787">
        <property type="protein sequence ID" value="PNT72787"/>
        <property type="gene ID" value="BRADI_2g49067v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72788">
        <property type="protein sequence ID" value="PNT72788"/>
        <property type="gene ID" value="BRADI_2g49067v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839809"/>
    <dbReference type="Gramene" id="KQK09608">
        <property type="protein sequence ID" value="KQK09608"/>
        <property type="gene ID" value="BRADI_2g49067v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72787">
        <property type="protein sequence ID" value="PNT72787"/>
        <property type="gene ID" value="BRADI_2g49067v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72788">
        <property type="protein sequence ID" value="PNT72788"/>
        <property type="gene ID" value="BRADI_2g49067v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839809"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001724_3_2_1"/>
    <dbReference type="OMA" id="CKAMTNI"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000020">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000082">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF39">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT72787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="484" mass="52946" checksum="E845FF0E51579553" modified="2012-06-13" version="1">MKQTVVLFAGAGVGHVTPMTELAYVFLKHGYDVTMVLLEPPFKSTDSGASFIERIAASNPSISFHVLPPLPAPDFAASGKHPFLLMLQLARDYNAPLEAFLRSIPRERLHSLVLDMFCVHAMDVGTAVGVPVYTFFASGASCLSVLTQFPALVAGRQSGLKDLGDTPLDFLGVPPMPASHLIRELLEHPEDEMCKAMTNIWKRNTETMGVLVNTFEALESRAVQSLRDPLCVPGRILPPVYCVGPLVSKGTAKDDSKAERNECLAWLDAQPDRSVVFLCFGSKGTLSADQLKEMAVGLERSGQRFLWSVRTPAGTKDPKKYFEVRPEADLDALLPEGFLERTKDRGLVVKSWAPQVDVLQHPATGAFVTHCGWNSTLEAVVAGVPMLCWPLEAEQKMNKVFMTEDMGVAVELEGYRTGFIKAGELEAKLRLVIEAEEGRQLRARVAARREEAQAALEEGGSSRAAFVQFLLDVENLAHGKELGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSD7</accession>
    <name>I1HSD7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NTF2-related export protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100830398</name>
        <name evidence="4" type="ORF">BRADI_2g52130v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564383.1">
        <property type="nucleotide sequence ID" value="XM_003564335.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSD7"/>
    <dbReference type="FunCoup" id="I1HSD7">
        <property type="interactions" value="2345"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HSD7"/>
    <dbReference type="EnsemblPlants" id="KQK10125">
        <property type="protein sequence ID" value="KQK10125"/>
        <property type="gene ID" value="BRADI_2g52130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830398"/>
    <dbReference type="Gramene" id="KQK10125">
        <property type="protein sequence ID" value="KQK10125"/>
        <property type="gene ID" value="BRADI_2g52130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830398"/>
    <dbReference type="eggNOG" id="KOG2104">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131642_0_0_1"/>
    <dbReference type="OMA" id="WEQVGTS"/>
    <dbReference type="OrthoDB" id="6507044at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000005">
        <property type="entry name" value="Nuclear transport factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="3">
        <location>
            <begin position="23"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK10125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="141" mass="15432" checksum="133C0C549AB8EEEA" modified="2012-06-13" version="1">MDGPQGKDVGSDGNGGGSEHDVVARAFVEYYYHTFDTDRAALAALYGSTSMLSFEGHRVAGAEEIGTKLAQLPFEQCRHSVVTVDCQPTPSFPAGILVFVSGNLRLAGEEHQLRFSQMFQLVPNEHGSFFVQNDIFRLNYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HUT3</accession>
    <name>I1HUT3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100827151</name>
        <name evidence="5" type="ORF">BRADI_2g59470v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567425.1">
        <property type="nucleotide sequence ID" value="XM_003567377.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753502.1">
        <property type="nucleotide sequence ID" value="XM_014898016.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HUT3"/>
    <dbReference type="STRING" id="15368.I1HUT3"/>
    <dbReference type="EnsemblPlants" id="KQK11323">
        <property type="protein sequence ID" value="KQK11323"/>
        <property type="gene ID" value="BRADI_2g59470v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827151"/>
    <dbReference type="Gramene" id="KQK11323">
        <property type="protein sequence ID" value="KQK11323"/>
        <property type="gene ID" value="BRADI_2g59470v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827151"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_32_0_1"/>
    <dbReference type="OMA" id="MEMMASK"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000583">
        <property type="entry name" value="Inactive leucine-rich repeat receptor-like protein kinase CORYNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051564">
        <property type="entry name" value="LRR_receptor-like_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48055">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48055:SF22">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE SERINE_THREONINE_TYROSINE-PROTEIN KINASE SOBIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094842" description="Protein kinase domain-containing protein" evidence="3">
        <location>
            <begin position="38"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="130"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK11323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="420" mass="45241" checksum="E48F7543735C5F78" modified="2012-06-13" version="1">MARGGGVVVGKKNPTKTLAAILLLLLLLAVFPRPAASQPLHSEPMSTQQSPPPPQQQSKIPHAQPGGGAARLRRIALGVLFGSLTGFLLSLALLYAIRLTVLHARDAPAIAKGPVSFTPQISPKNLLCALPAAQPLAHGTYHKLALDGGLTVAVKRLESVAANRPEASPSAPPPNGSKSDMRRVQRQLEVLARVRHQSVMSLKAYVREVDRLSLVYNFVPGGSLEDVMKRVRSQQLSLDWDTRSRIAAGVAKGLRYLHFECCPSILHCNLKPSNVMLDEGFEPILADCGVASLIDSGPADPESFGSVYAAPEFYQNSRYTDKCDVYAFGMILGVLLTGRDPKDPFFSGEAGRGGLARWLRHMQHSGEAKEALDSSILGEEVCEEEMLMAIRVAIVCLSDLPADRPSSDELVAMLAQLHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I1HX50</accession>
    <name>I1HX50_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100821615</name>
        <name evidence="2" type="ORF">BRADI_3g03660v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233800.1">
        <property type="nucleotide sequence ID" value="XM_010235498.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HX50"/>
    <dbReference type="STRING" id="15368.I1HX50"/>
    <dbReference type="EnsemblPlants" id="KQJ93289">
        <property type="protein sequence ID" value="KQJ93289"/>
        <property type="gene ID" value="BRADI_3g03660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821615"/>
    <dbReference type="Gramene" id="KQJ93289">
        <property type="protein sequence ID" value="KQJ93289"/>
        <property type="gene ID" value="BRADI_3g03660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821615"/>
    <dbReference type="eggNOG" id="ENOG502SB49">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098106_0_0_1"/>
    <dbReference type="OMA" id="YLCQPAF"/>
    <dbReference type="OrthoDB" id="1897212at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF139">
        <property type="entry name" value="OS02G0143300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ93289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="147" mass="15582" checksum="009BEFE573C6C742" modified="2012-06-13" version="1">MMMMGYLSLRAPRQLAGGRSSSNKAAERAALLGEEDAGAGAAVPRGYFAVYVGAEARRFVVPVSYLCQPAFRALMELAAEEFGFGQAGGLRFPCREEDFLAIVADLDAARADRAESRHRRRSSGAGAGAGKLGAMLFAHARAQVVQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXG3</accession>
    <name>I1HXG3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Wound induced protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100827700</name>
        <name evidence="2" type="ORF">BRADI_3g04585v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233840.1">
        <property type="nucleotide sequence ID" value="XM_010235538.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXG3"/>
    <dbReference type="STRING" id="15368.I1HXG3"/>
    <dbReference type="EnsemblPlants" id="KQJ93436">
        <property type="protein sequence ID" value="KQJ93436"/>
        <property type="gene ID" value="BRADI_3g04585v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827700"/>
    <dbReference type="Gramene" id="KQJ93436">
        <property type="protein sequence ID" value="KQJ93436"/>
        <property type="gene ID" value="BRADI_3g04585v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827700"/>
    <dbReference type="eggNOG" id="ENOG502R3PJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164353_0_0_1"/>
    <dbReference type="OMA" id="RMRKAYH"/>
    <dbReference type="OrthoDB" id="691528at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022251">
        <property type="entry name" value="DUF3774_wound-induced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33090">
        <property type="entry name" value="DUF3774 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12609">
        <property type="entry name" value="DUF3774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ93436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="8673" checksum="5030A6DF48B044D0" modified="2012-06-13" version="1">MASRKAPSLVVAASLGAVEALKDQAGLCRWGYALRSLHRRAVISVSLSETAPPAGASSRPTTSSSAAEVRLHKAHHLVCWGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYF7</accession>
    <name>I1HYF7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100837183</name>
        <name evidence="7" type="ORF">BRADI_3g07320v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 5"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571360.1">
        <property type="nucleotide sequence ID" value="XM_003571312.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYF7"/>
    <dbReference type="STRING" id="15368.I1HYF7"/>
    <dbReference type="EnsemblPlants" id="KQJ93895">
        <property type="protein sequence ID" value="KQJ93895"/>
        <property type="gene ID" value="BRADI_3g07320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837183"/>
    <dbReference type="Gramene" id="KQJ93895">
        <property type="protein sequence ID" value="KQJ93895"/>
        <property type="gene ID" value="BRADI_3g07320v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837183"/>
    <dbReference type="HOGENOM" id="CLU_089785_0_0_1"/>
    <dbReference type="OMA" id="QFSGESM"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HYF7">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811:SF26">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP16-1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="6">
        <location>
            <begin position="107"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ93895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="210" mass="22900" checksum="B94E863AC6B72F30" modified="2012-06-13" version="1">MELGSHTGFTSPFPCASLSPRCPYPPESKAFRPASRGFRAVCGVSRRRAVSEMAFLGAAASCVNLLAMPIPAQAAMQEPDVIRYRKLDSGVKLEDVVEGEGPEAREGDLVQFNYVCRRANGYFVHSTVDQFNGESKPVTLPLDGGEMIRGLKDVIIGMKAGGKRRALIPPEVGYVDESLQPVPEEFGPRRSLLSHAKEPLVFEVQLLKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HYI3</accession>
    <name>I1HYI3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100829405</name>
        <name evidence="1" type="ORF">BRADI_3g07530v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571473.1">
        <property type="nucleotide sequence ID" value="XM_003571425.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HYI3"/>
    <dbReference type="FunCoup" id="I1HYI3">
        <property type="interactions" value="2443"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HYI3"/>
    <dbReference type="EnsemblPlants" id="KQJ93924">
        <property type="protein sequence ID" value="KQJ93924"/>
        <property type="gene ID" value="BRADI_3g07530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829405"/>
    <dbReference type="Gramene" id="KQJ93924">
        <property type="protein sequence ID" value="KQJ93924"/>
        <property type="gene ID" value="BRADI_3g07530v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829405"/>
    <dbReference type="eggNOG" id="KOG1550">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066102_1_0_1"/>
    <dbReference type="OMA" id="VIDYCEP"/>
    <dbReference type="OrthoDB" id="2384430at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HYI3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45500">
        <property type="entry name" value="OS02G0202600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45500:SF1">
        <property type="entry name" value="OS02G0202600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ93924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="32014" checksum="4DADEA1A34E26401" modified="2012-06-13" version="1">MFAASARRVAAAISASSSSSSSSSSSSRATQLASALNHQRWMHDRNKKAMELVAKGWSALQEVDRVIDFADRNDKRLIPLLRGAKENFELALEIDNMNTHARCWLAKMHFKYHVPGACKAIGAALLVEAANMGDPEAQYELGCRLRIENDHVQSDHQAFHYIEKAVDQLHPGALYLLGAVYLTGDCVKRDIASAMWCFHRASEKGHAGAAIAYGSLLLKGAEVPEVITRLNSGKSPSTGKAWKRSIQQDPVKLAKEQFQIAAEAGCDLGLRWLKNLGDYEKQQELKQIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZL5</accession>
    <name>I1HZL5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nicotianamine synthase</fullName>
            <ecNumber evidence="2">2.5.1.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100822873</name>
        <name evidence="3" type="ORF">BRADI_3g10520v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Synthesizes nicotianamine, a polyamine which serves as a sensor for the physiological iron status within the plant, and/or might be involved in the transport of iron.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3 S-adenosyl-L-methionine = nicotianamine + 3 S-methyl-5'-thioadenosine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16481"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:58249"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.43"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nicotianamine synthase (NAS)-like family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.43" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572602.1">
        <property type="nucleotide sequence ID" value="XM_003572554.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZL5"/>
    <dbReference type="FunCoup" id="I1HZL5">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66354">
        <property type="protein sequence ID" value="PNT66354"/>
        <property type="gene ID" value="BRADI_3g10520v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822873"/>
    <dbReference type="Gramene" id="PNT66354">
        <property type="protein sequence ID" value="PNT66354"/>
        <property type="gene ID" value="BRADI_3g10520v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822873"/>
    <dbReference type="eggNOG" id="ENOG502QTU6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031919_0_0_1"/>
    <dbReference type="OMA" id="YDWSESA"/>
    <dbReference type="OrthoDB" id="612523at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030410">
        <property type="term" value="F:nicotianamine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030418">
        <property type="term" value="P:nicotianamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004298">
        <property type="entry name" value="Nicotian_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266:SF23">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32266">
        <property type="entry name" value="NICOTIANAMINE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03059">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51142">
        <property type="entry name" value="NAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU368095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT66354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="335" mass="36056" checksum="80ED6709AF68029D" modified="2012-06-13" version="1">MEAQNQEVAALVQKITGLQAAIAKLPSLSPSPQVDALFTELVTACVPPSPVDVTKLGPEAQRMREELIRLCSTAEGHLEAHYADKLAAFDNPLDHLDCFPYYSNYINLSKLEYDLLARYMPSSSGIEPARVAFVGSGPLPFTSLVLAARHLPNTLFDNYDWSESANERARKLVRADKDVGARMSFHTADVAKLTGELGKYDVLFLAALVGMAAEDKATVIAHLGAHMADGAALVVRSAHGARGFLYPIVDPELITQGGFEVLAVYHPDDEVVNSVIIARKAADARGLGKEENGRGARGHAPVPVPVVSPPCRCCSKMEAGMLEKSVEFATTELSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1B1</accession>
    <name>I1I1B1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100830340</name>
        <name evidence="5" type="ORF">BRADI_3g16170v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573442.1">
        <property type="nucleotide sequence ID" value="XM_003573394.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1B1"/>
    <dbReference type="STRING" id="15368.I1I1B1"/>
    <dbReference type="EnsemblPlants" id="KQJ95272">
        <property type="protein sequence ID" value="KQJ95272"/>
        <property type="gene ID" value="BRADI_3g16170v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830340"/>
    <dbReference type="Gramene" id="KQJ95272">
        <property type="protein sequence ID" value="KQJ95272"/>
        <property type="gene ID" value="BRADI_3g16170v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830340"/>
    <dbReference type="eggNOG" id="KOG3295">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075696_1_0_1"/>
    <dbReference type="OMA" id="HWHKRIK"/>
    <dbReference type="OrthoDB" id="10264538at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000003">
        <property type="entry name" value="60S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ95272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="208" mass="23930" checksum="AE6A09EECDF6DF2A" modified="2012-06-13" version="1">MVKHNNVIPNGHFKKHWQNYVKTWFNQPARKQRRRIARQKKAVKIFPRPTSGPLRPIVQCQTLKYNMKARAGRGFTLEELKAAGIPKKLAPTIGISVDHRRKNRSLEGMQSNVQRLKTYKAKLVIFPRRARKVKAGDSTPEELATATQVQGDFMPIARGEKRSVEVVKVTDEMKAFKAYGKLRVERMNQRQVGARMKKAAEAEKDEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1I3W5</accession>
    <name>I1I3W5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100844904</name>
        <name evidence="3" type="ORF">BRADI_3g26160v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573907.1">
        <property type="nucleotide sequence ID" value="XM_003573859.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I3W5"/>
    <dbReference type="FunCoup" id="I1I3W5">
        <property type="interactions" value="2166"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I3W5"/>
    <dbReference type="EnsemblPlants" id="KQJ96636">
        <property type="protein sequence ID" value="KQJ96636"/>
        <property type="gene ID" value="BRADI_3g26160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844904"/>
    <dbReference type="Gramene" id="KQJ96636">
        <property type="protein sequence ID" value="KQJ96636"/>
        <property type="gene ID" value="BRADI_3g26160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844904"/>
    <dbReference type="eggNOG" id="KOG1947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="ACANLHI"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I3W5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000357">
        <property type="entry name" value="F-box/LRR-repeat protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055312">
        <property type="entry name" value="FBL15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709:SF57">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709">
        <property type="entry name" value="OS10G0396666 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="221"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ96636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1017" mass="111420" checksum="03E960724EBA6EBB" modified="2012-06-13" version="1">MADGGRMKGVEGSGEGAGQDEERDEDAREEPELALSLGRRGWHLPPQPPPAPALRWSMVFSAWDPDASGSSRAAERIWDSPPVPPLRFPDMWRGVRADGDAGGAGETEEHNDDEEEDEIEDGDRDLQNKRPKVRGFGEESPQHSGVNASFFGFEPPHLNAFAEHDHLKLSHCPENELDFGLSLFSNDGGNEIQRETNNQEAGGLEKSGDRNAEGVAIRMDLSDDLLHLIFSFLCQKDLCRAGAACKQWQSACIHEDFWKCLKFENTRISLQNFVNICHRYQNVTDLNLSGVSDADLLVMDAITFLRHLKTLTMGKGHLGEAFFQTLAECPLLNTLTVSDASLGSGIQEVTVNHDGLRELQIVKCRVLRVSIRCPQLQLLSLRRTGMAHVSLNCPQLLELDFQSCHKLSDTAIRQAATACPLLASLDMSSCSCVTDETLREIASACQNLSVLDASNCPNISFESVKLPMLIDLRLSSCEGITSASIGAIYYSRILEALQLDNCSLLTSVSLDMPHLRNISLVHLRKFADLNLRSPVLSYIKVSRCSVLRCVSITSNALQKLVLQKQESLSSLSLQCHNLIDVDLSDCESLTNEICEVFSDGGGCPMLRSLILDNCESLCVVGLNNSSLVNLSLAGCRSMTFLKLACPRLQMVNLDGCDHLERASFCPVGLESLNLGICPKLTVLHIEAPNMSILELKGCGVLSQSSINCPRLTSLDASFCRQLMDDSLSRTAEACPLIEHLILSSCLSIDITGLSSLNCLHMLALLDLSYTFLINLKPVFDSCPQLKVLKLSACKYLSDSSLDPLYRECALPMLTELDLSYSSIGQTAIEELLACCTNLVNVNLNGCTNLHELVCGSDYCSSVDMPIDVCPSYSAPDKTKEINESPYSLLEVLNCTGCPNIKKVVISSVANYLNLSKINLNLSAHLKEVDLKCSNLYNLNLSNCSSLEILKLDCPRLANLQLLACTMLQEEELESALSFCGSLEILNVHSCPKINALDFGRLQAVCPTLKRIQSSLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5M4</accession>
    <name>I1I5M4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutathione S-transferase</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g31870v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Tau family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5M4"/>
    <dbReference type="STRING" id="15368.I1I5M4"/>
    <dbReference type="EnsemblPlants" id="KQJ97561">
        <property type="protein sequence ID" value="KQJ97561"/>
        <property type="gene ID" value="BRADI_3g31870v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97561">
        <property type="protein sequence ID" value="KQJ97561"/>
        <property type="gene ID" value="BRADI_3g31870v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0406">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_18_0_1"/>
    <dbReference type="InParanoid" id="I1I5M4"/>
    <dbReference type="OMA" id="CSCAEVM"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000044">
        <property type="entry name" value="Glutathione S-transferase GSTU6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000023">
        <property type="entry name" value="Probable glutathione S-transferase GSTU6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF550">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="11"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="98"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ97561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="228" mass="24696" checksum="45CF4345DA2E052F" modified="2012-06-13" version="1">MAAAAGVGEGEEVKLLGCWDSPFVNRVQIALNLKGIPYEYVEEDLHCKGAVLLASNPVHKKVPVLIHNGKPIPESQVILQYIDEAWAGSDGPSLLPADPLQRAAARFWAAFVDDKVGAPWFTILFARETEEKKEAAARAVAAMETLEGTLGEEKAFFGGDGIGFVDVVLGSYLGWFVVIEKMIGVKLLDAARMPALAAWAERFKAADAVKGVLPEDVDKVLEFLQTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6P2</accession>
    <name>I1I6P2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Seed maturation protein PM41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100822566</name>
        <name evidence="2" type="ORF">BRADI_3g34870v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574420.1">
        <property type="nucleotide sequence ID" value="XM_003574372.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6P2"/>
    <dbReference type="FunCoup" id="I1I6P2">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I6P2"/>
    <dbReference type="EnsemblPlants" id="KQJ98093">
        <property type="protein sequence ID" value="KQJ98093"/>
        <property type="gene ID" value="BRADI_3g34870v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822566"/>
    <dbReference type="Gramene" id="KQJ98093">
        <property type="protein sequence ID" value="KQJ98093"/>
        <property type="gene ID" value="BRADI_3g34870v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822566"/>
    <dbReference type="eggNOG" id="ENOG502S4IR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191789_0_0_1"/>
    <dbReference type="OMA" id="RPRMDIH"/>
    <dbReference type="OrthoDB" id="668318at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd23010">
        <property type="entry name" value="PM41-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36012">
        <property type="entry name" value="OS01G0654400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36012:SF2">
        <property type="entry name" value="OS08G0385000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ98093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="8335" checksum="8408BC64B923513E" modified="2012-06-13" version="1">MSGAQGAQPKGAFTATTYTTAGTGEGESRQTPKTELRSSEDEHGLPVKRLEDKVEDAAGKGGPVFGAGAEDGKPDLGVTGTGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IF69</accession>
    <name>I1IF69_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100846011</name>
        <name evidence="4" type="ORF">BRADI_3g59080v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT69627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573094.3">
        <property type="nucleotide sequence ID" value="XM_003573046.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IF69"/>
    <dbReference type="STRING" id="15368.I1IF69"/>
    <dbReference type="EnsemblPlants" id="PNT69627">
        <property type="protein sequence ID" value="PNT69627"/>
        <property type="gene ID" value="BRADI_3g59080v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846011"/>
    <dbReference type="Gramene" id="PNT69627">
        <property type="protein sequence ID" value="PNT69627"/>
        <property type="gene ID" value="BRADI_3g59080v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846011"/>
    <dbReference type="eggNOG" id="KOG1603">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100095_0_0_1"/>
    <dbReference type="OMA" id="NPAGYYH"/>
    <dbReference type="OrthoDB" id="666972at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF145">
        <property type="entry name" value="COPPER ION BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003643957" description="HMA domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="3">
        <location>
            <begin position="46"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT69627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="175" mass="19303" checksum="D74F70B45D670E66" modified="2012-06-13" version="1">MGGSVKRLVLGLLGAVGGGGGHGGKKRTRRRHQHQQQLQLQLQLQTVVVELRVRMDCERCERQVKKALAGITGVEHVEVSRRQQRVTVTGNVDPHKVLRQAQLTGKKAELWRTQNNPAYSSTADMALYGMGAAAQAHERWAAAVPYQRNPDATTLSAEHITDLFSDDNPNACFIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1IGK9</accession>
    <name>I1IGK9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YTH domain-containing family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100821870</name>
        <name evidence="7" type="ORF">BRADI_4g02150v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the YTHDF family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579159.1">
        <property type="nucleotide sequence ID" value="XM_003579111.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IGK9"/>
    <dbReference type="SMR" id="I1IGK9"/>
    <dbReference type="STRING" id="15368.I1IGK9"/>
    <dbReference type="EnsemblPlants" id="KQJ85870">
        <property type="protein sequence ID" value="KQJ85870"/>
        <property type="gene ID" value="BRADI_4g02150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821870"/>
    <dbReference type="Gramene" id="KQJ85870">
        <property type="protein sequence ID" value="KQJ85870"/>
        <property type="gene ID" value="BRADI_4g02150v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821870"/>
    <dbReference type="eggNOG" id="KOG1901">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017795_5_1_1"/>
    <dbReference type="OMA" id="YDAPKYS"/>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.590.10:FF:000001">
        <property type="entry name" value="YTH domain family 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF99">
        <property type="entry name" value="YTH DOMAIN-CONTAINING PROTEIN ECT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="YTH" evidence="6">
        <location>
            <begin position="441"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="265"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="622"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="199"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="624"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ85870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="656" mass="71358" checksum="86B2FFE63B063051" modified="2012-06-13" version="1">MPYGSANAGAVLPGAELHADMQNLTLEGNKEGSDAVANKASGLPYGSASNGNSSQSVEPHVDRSITPLLQEAMDPNFFYQPNGYPSPAYYYPSGYDGSTNEWDSSYAGHEGMEVPQNVYGDMYHGYGYAPYGPYPSGSPVPTVGHDGQSYGTQHYQYPTQYYQQPAQTNAVHGVNGANSPSELRPVTTHQARAPVASAKANNGTASGIANANTSSLSRKQTHQNVSVTNNVPYGRGPSQGGPSASNFGHNGLRSPVQWYDAPVYSNGHQRSTASSTSYGSNSSSAKNQSHRPTTNLMGMRTQMPSSGVGLTSPSYPSRAYPDSRLYGQYGHYGNTLKAGLGFGSNVYNSRNNGQWGVVDTAKYKPRSRAPFGFGSENQDGFTELNRGPRSGGFRHQKPFGPTVTIAVKGQALPSVGKQENCVLPDKSQFNQESFPATYRDAKFFVIKSYSEDDVHKSIKYNVWASTPNGNKKLDSGYREAQEKSSECPVFLFFSVNTSGQFVGVAEMVGPVDFDKTVDYWQQDKWNGCFSIKWHIVKDIPNNILKHITLDNNDNKPVTNSRDTQEVKLEQGLQMLKIFKDHSSKTSILDDFAFYENRQKLMQEKRAKQQLLQGQGCDEKEKNALNGSSAVQKQTLNEGVVLTAPKPAVESGAANGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1II65</accession>
    <name>I1II65_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cornichon family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100831579</name>
        <name evidence="7" type="ORF">BRADI_4g06710v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576073.1">
        <property type="nucleotide sequence ID" value="XM_003576025.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237232.1">
        <property type="nucleotide sequence ID" value="XM_010238930.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1II65"/>
    <dbReference type="STRING" id="15368.I1II65"/>
    <dbReference type="EnsemblPlants" id="KQJ86622">
        <property type="protein sequence ID" value="KQJ86622"/>
        <property type="gene ID" value="BRADI_4g06710v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ86623">
        <property type="protein sequence ID" value="KQJ86623"/>
        <property type="gene ID" value="BRADI_4g06710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831579"/>
    <dbReference type="Gramene" id="KQJ86622">
        <property type="protein sequence ID" value="KQJ86622"/>
        <property type="gene ID" value="BRADI_4g06710v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ86623">
        <property type="protein sequence ID" value="KQJ86623"/>
        <property type="gene ID" value="BRADI_4g06710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831579"/>
    <dbReference type="eggNOG" id="KOG2729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112942_3_0_1"/>
    <dbReference type="OMA" id="FAVFHVI"/>
    <dbReference type="OrthoDB" id="434393at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ86622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17397" checksum="93EF47E309C3539B" modified="2012-06-13" version="1">MSVELILWLFSFASVMVLVGLTAYQLICLSDLEFDYINPYDSSSRINAVVIIEYALQAVLCAAFLLTLNWFPFLVMAPVTYYHVKLYMNRKHLVDVTEIFRQLHGEKKYRMIKLAFYFGLFIITIYRLVMTAVLLFIDEDANLVETRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1IK11</accession>
    <name>I1IK11_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC104584922</name>
        <name evidence="3" type="ORF">BRADI_4g12290v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239048.2">
        <property type="nucleotide sequence ID" value="XM_010240746.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IK11"/>
    <dbReference type="STRING" id="15368.I1IK11"/>
    <dbReference type="EnsemblPlants" id="KQJ87613">
        <property type="protein sequence ID" value="KQJ87613"/>
        <property type="gene ID" value="BRADI_4g12290v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584922"/>
    <dbReference type="Gramene" id="KQJ87613">
        <property type="protein sequence ID" value="KQJ87613"/>
        <property type="gene ID" value="BRADI_4g12290v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584922"/>
    <dbReference type="eggNOG" id="ENOG502RY48">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020188_5_3_1"/>
    <dbReference type="OMA" id="TACWERS"/>
    <dbReference type="OrthoDB" id="591587at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004158">
        <property type="entry name" value="DUF247_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170:SF18">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31170">
        <property type="entry name" value="BNAC04G53230D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03140">
        <property type="entry name" value="DUF247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ87613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="477" mass="53541" checksum="96968285E77D4661" modified="2012-06-13" version="1">MDVQGPQQEQDQVEEEDDESLIITEILTAALPKLVDTSVACLISWAGDDIVDRGHYYEPSTVRIGPFHRQDPCKRSADRTELKKKLVLRGLLTSSKEEGRREELRSHLEAMERMEADARRCYNRSFSWMTSKEFARMLLLDGCFLYSRFSGFVSGSGDDDDIAVDRDVMFLLENQIPFFVLEKIHQRFNTSTSGNKVLVDKVTKRVQRVLQRSNYIALANVNVPSSPPCHLLHLLHMYFTPTSSTLDLDSTSSPKQSTEPRPPALVRRQGDGQHSIRVPVRWRTATQYHAAGVRFVKRKLGGNGNGEARSILDVDLIKDRLHVPCLTVDANTLRLLRNMVALEQKSLQQQRTSHVTAYCLFLSQLASTEEDVELLVSKGIIVHLLRSNGDVAEGLAGLCDGVVIDPDAGNDYLLRSKYEALEKLIVVCSGSGGRKSMAWLRSHSKCLLVAALVLLLFLWTAQQSVFSALRNCKCRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IMT7</accession>
    <name>I1IMT7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fungal lipase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100824537</name>
        <name evidence="8" type="ORF">BRADI_4g23367v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577748.1">
        <property type="nucleotide sequence ID" value="XM_003577700.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IMT7"/>
    <dbReference type="FunCoup" id="I1IMT7">
        <property type="interactions" value="1396"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IMT7"/>
    <dbReference type="EnsemblPlants" id="KQJ89079">
        <property type="protein sequence ID" value="KQJ89079"/>
        <property type="gene ID" value="BRADI_4g23367v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824537"/>
    <dbReference type="Gramene" id="KQJ89079">
        <property type="protein sequence ID" value="KQJ89079"/>
        <property type="gene ID" value="BRADI_4g23367v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824537"/>
    <dbReference type="eggNOG" id="ENOG502R42M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016367_2_0_1"/>
    <dbReference type="OMA" id="YKIFDKW"/>
    <dbReference type="OrthoDB" id="426718at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IMT7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009862">
        <property type="term" value="P:systemic acquired resistance, salicylic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041266">
        <property type="entry name" value="EDS1_EP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47413">
        <property type="entry name" value="LIPASE-LIKE PAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47413:SF2">
        <property type="entry name" value="LIPASE-LIKE PAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18117">
        <property type="entry name" value="EDS1_EP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="6">
        <location>
            <begin position="132"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="EDS1 EP" evidence="7">
        <location>
            <begin position="430"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ89079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="655" mass="70504" checksum="34B86AA7E3A9515B" modified="2012-06-13" version="1">MEDAEEISMFETSHVLGALLASSPLLARAWDRCAAAAAMGAASPGFVYGGGGGDDGGTVYVAFSGVQAALSVAGAAVASGADVFAPVGLAGDAAGARAFPQLAAAEPDAAAGDPVAVQALALRCFLKLCGSPEFQMLLNQIRGKGVVFTGHSLGGAIATLAALHYLCISSSSSAYATAPPVLCVTFGSPLLGNEALSRAILRERWGGNFCHVVSQHDVVPRLLFCPLDAVPVRIIVGLQLQQWPGCTRHVGTVTNSVEDAEQEALQQLIQAHSRVVAMEQKLAAPEMRGGSPYRPFGAYVLCSPDGAACVDGLTAAVQMLYATFAAKCVSGSVKSLEAAHSCYGDLVLKMPQHLVLKRRPRAVDVLAAVSNSNYDAGISLALEASGIDGEATGATTVRHWLKASKRAGRSPSLNCAGLATRLGRITPCRAQIEWYKASFDGDTGYYDAFKQRRSPKKFHKANIYRIKLGQFWDGVLTMLETSQLPHDFHRRAKWVNAARFYQLLVEPLDIADYHRNNLHKTRGSYITHGRERRYELFDKWWKGKGTFTGCTSDTSTTASRTRSKYAGLTQDPCFWARVEDAREQTESAAAGHDAVALATKLESLREFEHYAAELVESKEVSIDVLAPQSSYSLWLEEWKELKLSDEARTSSLFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1INK3</accession>
    <name>I1INK3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC104581268</name>
        <name evidence="5" type="ORF">BRADI_4g25797v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239300.1">
        <property type="nucleotide sequence ID" value="XM_010240998.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024319460.1">
        <property type="nucleotide sequence ID" value="XM_024463692.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024319461.1">
        <property type="nucleotide sequence ID" value="XM_024463693.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024319462.1">
        <property type="nucleotide sequence ID" value="XM_024463694.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024319463.1">
        <property type="nucleotide sequence ID" value="XM_024463695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INK3"/>
    <dbReference type="FunCoup" id="I1INK3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ89451">
        <property type="protein sequence ID" value="KQJ89451"/>
        <property type="gene ID" value="BRADI_4g25797v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581268"/>
    <dbReference type="Gramene" id="KQJ89451">
        <property type="protein sequence ID" value="KQJ89451"/>
        <property type="gene ID" value="BRADI_4g25797v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104581268"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_0_6_1"/>
    <dbReference type="OMA" id="AEVHCRI"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000806">
        <property type="entry name" value="Os11g0114800 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000333">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001098">
        <property type="entry name" value="Putative pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF344">
        <property type="entry name" value="OS07G0636900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="159"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="252"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="322"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ89451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="43082" checksum="AA766FCA91B498FF" modified="2012-06-13" version="1">MHPTPPPAPGRSNSPAAASRLLPRRGSAAAGHQLHAVLIKHGLLHHPASLRALLSRLPPSSASTLALLLSAAPCSLSPPLYSPAIAALASSPYPAASLAVFNRVSRLSLPIPLPAFPALLKSCARAFNLCAAADGGGEGFVSKGMELHGCVLKLGCGADRYVQNALVSMYGKLGRLGDARKVFDGMPARNAVSWNALVAAHGDVGDSRGAERVSQEMPERSVSWWNAEITRHARVGDMEAAARVFREMPGRDAVSWNSLIGSYAKLGQCARALKVFREMQDSGVEPTELTLVSVLGACTEIGELELGKGVHGYLSSKGVLADGYVGNALVDMYAKCGSLELARQVFESMSTRDITCWNAMIVGLSVHGYSREALELFNVMRVEPDHVTFLGVLIVCSHSGLVDEGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IR20</accession>
    <name>I1IR20_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cellulose synthase-like protein E6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100845624</name>
        <name evidence="15" type="ORF">BRADI_4g33090v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Thought to be a Golgi-localized beta-glycan synthase that polymerize the backbones of noncellulosic polysaccharides (hemicelluloses) of plant cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase-like E subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576649.1">
        <property type="nucleotide sequence ID" value="XM_003576601.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IR20"/>
    <dbReference type="STRING" id="15368.I1IR20"/>
    <dbReference type="EnsemblPlants" id="KQJ90650">
        <property type="protein sequence ID" value="KQJ90650"/>
        <property type="gene ID" value="BRADI_4g33090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845624"/>
    <dbReference type="Gramene" id="KQJ90650">
        <property type="protein sequence ID" value="KQJ90650"/>
        <property type="gene ID" value="BRADI_4g33090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845624"/>
    <dbReference type="eggNOG" id="ENOG502QS7H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001418_3_3_1"/>
    <dbReference type="OMA" id="CEENTSW"/>
    <dbReference type="OrthoDB" id="72851at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016759">
        <property type="term" value="F:cellulose synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009833">
        <property type="term" value="P:plant-type primary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000138">
        <property type="entry name" value="Cellulose synthase isolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000173">
        <property type="entry name" value="Cellulose synthase-like protein E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="641"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="671"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="704"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="439"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQJ90650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="725" mass="81749" checksum="122CF6F6D5403CBC" modified="2012-06-13" version="1">MERLFATEIIGGRALYKLHAVTVAVGICLVLYYRATRVPEQGQGRAAWLGMLAAELCYAAYWVVTQSVRWCPVRRIPFRDRLAARYGERLPCVDIFVCTADPHSEPPSLVISTVLSLMAYNYPTEKISVYLSDDGGSILTFYALWEASLFAKHWLPFCKRYNIEPRSPAAYFSESDGHQDLCTTKEWSLIKDMYEEMTERIDTVVESGKIAEEIKEKHKGFGEWSPEITSKNHQPIVQILVNSKDGNAVDNDGNVLPTLVYMAREKRPQHHHNFKAGAMNALIRVSSVISNSPIIMNVDCDMYSNSSDTITDALCFFLDEEMGHKIGFVQYPQNYNNMTKNNIYGNSLQVINKVELNGLDSVGGPLYIGTGCFHRREILCGRKFTEDYREDWNGGIKDKMQAHADEIEEKAKSLAACTYEHDTQWGDEIGLKYGCPVEDVITGLAIHCRGWGSVCNNPTRPAFVGVGPTTLAQTLLQHKRWSEGNFSIFLSKYCPFLFGHGKITLQHQMGYCIYGLWAPNSLPTLYYLIIPPLALFKGTPLFPEITSPWIIPFISVFCVKNLYSLCESLLCGDTLKGWWNGQRMWMVKRITSYLYGVIDTVRKLIGLSKMSFAVSSKVSDEDESKRYEQEIMEFGSSDPEYVIIATIALLNLVCLAGGLSQMMTGERGIRFNVFCPQLILCGMLVITSVPFYEAMFLRKDKGRIPFSVTLASIGFVMLTFLVPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1ISN9</accession>
    <name>I1ISN9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100834661</name>
        <name evidence="6" type="ORF">BRADI_4g37550v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578616.1">
        <property type="nucleotide sequence ID" value="XM_003578568.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ISN9"/>
    <dbReference type="STRING" id="15368.I1ISN9"/>
    <dbReference type="EnsemblPlants" id="KQJ91418">
        <property type="protein sequence ID" value="KQJ91418"/>
        <property type="gene ID" value="BRADI_4g37550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834661"/>
    <dbReference type="Gramene" id="KQJ91418">
        <property type="protein sequence ID" value="KQJ91418"/>
        <property type="gene ID" value="BRADI_4g37550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834661"/>
    <dbReference type="eggNOG" id="KOG3409">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067135_3_0_1"/>
    <dbReference type="OMA" id="PMVPVGW"/>
    <dbReference type="OrthoDB" id="440760at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05791">
        <property type="entry name" value="S1_CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000223">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019495">
        <property type="entry name" value="EXOSC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE CSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10447">
        <property type="entry name" value="EXOSC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exosome complex component N-terminal" evidence="5">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Exosome complex component CSL4 C-terminal" evidence="4">
        <location>
            <begin position="103"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ91418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="21152" checksum="D56F7B027719DAA1" modified="2012-06-13" version="1">MAATAMEHDGGVEVVTPGELLGTSSSFVAGHGAYADGRSVRASVTGHRRIVPPSPDSPDKRSTVEVVGHKAHGAVPQPGSVVIARVTKVMARVASADIMCVDSKAVKEKFTGMIRQQDVRATEIDKVDMYQSYRPGDIVRALVLSLGDARAYYLSTAKNELGVVSAQSIAGGMLVPTSWTEMQCELTGQIEQRKVAKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITL5</accession>
    <name>I1ITL5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100826901</name>
        <name evidence="12" type="ORF">BRADI_4g40250v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576960.1">
        <property type="nucleotide sequence ID" value="XM_003576912.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITL5"/>
    <dbReference type="EnsemblPlants" id="KQJ91873">
        <property type="protein sequence ID" value="KQJ91873"/>
        <property type="gene ID" value="BRADI_4g40250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826901"/>
    <dbReference type="Gramene" id="KQJ91873">
        <property type="protein sequence ID" value="KQJ91873"/>
        <property type="gene ID" value="BRADI_4g40250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826901"/>
    <dbReference type="eggNOG" id="ENOG502S2I7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025298_1_1_1"/>
    <dbReference type="OMA" id="GGVECHN"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009640">
        <property type="term" value="P:photomorphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19821">
        <property type="entry name" value="Bbox1_BBX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000589">
        <property type="entry name" value="B-box zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051979">
        <property type="entry name" value="B-box_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049808">
        <property type="entry name" value="CONSTANS-like_Bbox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31832">
        <property type="entry name" value="B-BOX ZINC FINGER PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31832:SF63">
        <property type="entry name" value="B-BOX ZINC FINGER PROTEIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="67"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="170"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ91873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="222" mass="22842" checksum="97F8D164BF87576F" modified="2012-06-13" version="1">MKIGCDACGRAAAAVLCCADEAALCRRCDAAVHSANRLAGRHQRVELLSSSSTGAGAGEGDGTHPACDICQEKTGYFFCVEDRALLCRSCDVAVHTATAQASSHRRFLITGVRVGGSANAQQDRHIVSPSSSSANGSSSINVPPASSRTRGLADNDHLSAQTAARLLGVGGEDEEEAAAAGQQQWPWSDIFADDSGVGNGMDDRQFYNGLVSEPAGSSGLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IUL5</accession>
    <name>I1IUL5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Saccharopine dehydrogenase NADP binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100834860</name>
        <name evidence="3" type="ORF">BRADI_4g43280v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578957.1">
        <property type="nucleotide sequence ID" value="XM_003578909.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IUL5"/>
    <dbReference type="FunCoup" id="I1IUL5">
        <property type="interactions" value="663"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IUL5"/>
    <dbReference type="EnsemblPlants" id="KQJ92381">
        <property type="protein sequence ID" value="KQJ92381"/>
        <property type="gene ID" value="BRADI_4g43280v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834860"/>
    <dbReference type="Gramene" id="KQJ92381">
        <property type="protein sequence ID" value="KQJ92381"/>
        <property type="gene ID" value="BRADI_4g43280v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834860"/>
    <dbReference type="eggNOG" id="ENOG502QQSS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032858_1_0_1"/>
    <dbReference type="OMA" id="YWFDMPE"/>
    <dbReference type="OrthoDB" id="10268090at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IUL5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43796">
        <property type="entry name" value="CARBOXYNORSPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43796:SF2">
        <property type="entry name" value="CARBOXYNORSPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="2">
        <location>
            <begin position="41"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="427" mass="45754" checksum="DADF3DA54E3E85D1" modified="2012-06-13" version="1">MAAMVRACMPLRTPPAASASAAAPAASTDAAPGRPRSSARVLVLGGTGRVGGSTATALSKLCPDLSILVGGRNREKGESLASKLGEQSKFVEIDTGNAAMLEEALQGVDLVVHTAGPFQREAECTVLQAAISTKTAYIDVCDDMDYSWRAKAFHEEAKAQGVPAITTAGIYPGVSNVMAAELVNAARSENEEPERLRFFYYTAGSGGAGPTILATSFLLLGEDVIAYNKGEEIKLKPYSGALNIDFGKGVRKRDVYLLNLPEVKSAHKFLGVPTVSARFGTAPFFWNWGMQAFANFLPVEFLRDKDKVRELVELVDPLVRAIDGIAGERVSMRVDLECSNGRSTIGLFSHKKLSVSVGYSVAAFVLAVLEGSTQPGVWFPEEPEGIAVESRKLLLERASQGTTNFVMNKPSWMVETDPKEVILGIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IXW0</accession>
    <name>I1IXW0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ZF-HD dimerization-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100836297</name>
        <name evidence="14" type="ORF">BRADI_5g10470v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homo- and heterodimer with other ZFHD proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579781.1">
        <property type="nucleotide sequence ID" value="XM_003579733.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IXW0"/>
    <dbReference type="STRING" id="15368.I1IXW0"/>
    <dbReference type="EnsemblPlants" id="KQJ82687">
        <property type="protein sequence ID" value="KQJ82687"/>
        <property type="gene ID" value="BRADI_5g10470v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836297"/>
    <dbReference type="Gramene" id="KQJ82687">
        <property type="protein sequence ID" value="KQJ82687"/>
        <property type="gene ID" value="BRADI_5g10470v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836297"/>
    <dbReference type="eggNOG" id="ENOG502SNDM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039237_4_0_1"/>
    <dbReference type="OMA" id="HKTHLAS"/>
    <dbReference type="OrthoDB" id="636896at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006455">
        <property type="entry name" value="Homeodomain_ZF_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006456">
        <property type="entry name" value="ZF_HD_homeobox_Cys/His_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01565">
        <property type="entry name" value="homeo_ZF_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01566">
        <property type="entry name" value="ZF_HD_prot_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948">
        <property type="entry name" value="ZINC-FINGER HOMEODOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948:SF128">
        <property type="entry name" value="ZINC-FINGER HOMEODOMAIN PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04770">
        <property type="entry name" value="ZF-HD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51523">
        <property type="entry name" value="ZF_HD_DIMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZF-HD dimerization-type" evidence="13">
        <location>
            <begin position="23"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="127"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="246"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="271"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ82687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="285" mass="29312" checksum="D30A316202F223D8" modified="2012-06-13" version="1">MMDQLSLVPYEGGSGVGGAGGKYKECMRNHAAAMGGQAFDGCGEYMSASPDSLSCAACGCHRSFHRRQAAGGSLTGSPVFFRPPPPPPHTHHGVLQGFLASAPPPPPQLALPYHAVPTAAWHHGLGLDPAHTARAGSETPPRAEDCSPGSGSGGPGAGAGMFGRKRVRTKFTPEQKEQMRAFAEKQGWRINRDDGGALDRFCLEIGVKRNVLKVWMHNHKTHLASPTFRSSAAAVAAAAGINPVAGAGPGIGITGVGTGDGDDEDTDDSPPRAAVSSPSPSPISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1IYL6</accession>
    <name>I1IYL6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100826512</name>
        <name evidence="3" type="ORF">BRADI_5g12730v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581326.1">
        <property type="nucleotide sequence ID" value="XM_003581278.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014751685.1">
        <property type="nucleotide sequence ID" value="XM_014896199.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311777.1">
        <property type="nucleotide sequence ID" value="XM_024456009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IYL6"/>
    <dbReference type="STRING" id="15368.I1IYL6"/>
    <dbReference type="EnsemblPlants" id="KQJ83034">
        <property type="protein sequence ID" value="KQJ83034"/>
        <property type="gene ID" value="BRADI_5g12730v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61247">
        <property type="protein sequence ID" value="PNT61247"/>
        <property type="gene ID" value="BRADI_5g12730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826512"/>
    <dbReference type="Gramene" id="KQJ83034">
        <property type="protein sequence ID" value="KQJ83034"/>
        <property type="gene ID" value="BRADI_5g12730v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61247">
        <property type="protein sequence ID" value="PNT61247"/>
        <property type="gene ID" value="BRADI_5g12730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826512"/>
    <dbReference type="eggNOG" id="ENOG502QTIQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072195_1_0_1"/>
    <dbReference type="OMA" id="AMHEIDI"/>
    <dbReference type="OrthoDB" id="2017234at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47489">
        <property type="entry name" value="ACYL-COA N-ACYLTRANSFERASES (NAT) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47489:SF2">
        <property type="entry name" value="GCN5-RELATED N-ACETYLTRANSFERASE 5, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="128"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT61247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="301" mass="32684" checksum="A8C364D5CB03EFCF" modified="2012-06-13" version="1">MAASPALLSLSLAPAVSRHPHLLFSSTHSPRHLRLALPSASSSSSPSPSPSPSPRGGGVFLSPSALSQLDALAAFRYAHSFPHGILTVRALTAPDDAEADALVGLLASSFAEDVRWAPAQRYAQLLAFVIRRYLHERRGLAPHAAVLVGFYKPAVAEEGEEGEDGEDEGEMACTAEVSLDAVGAPGAPPTPTPPLEFPYICNMTVKTSLRRRGIGKQLLKACEDLAVKMDAKRRVYLHCRIIDQIPFNMYKKAGYEVVQTDSIFVWLSLQKRKHLMRKELPQVASGSNFTTIDFDDNVLMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZ22</accession>
    <name>I1IZ22_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100830788</name>
        <name evidence="15" type="ORF">BRADI_5g13960v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580015.1">
        <property type="nucleotide sequence ID" value="XM_003579967.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZ22"/>
    <dbReference type="FunCoup" id="I1IZ22">
        <property type="interactions" value="1528"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IZ22"/>
    <dbReference type="EnsemblPlants" id="KQJ83256">
        <property type="protein sequence ID" value="KQJ83256"/>
        <property type="gene ID" value="BRADI_5g13960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830788"/>
    <dbReference type="Gramene" id="KQJ83256">
        <property type="protein sequence ID" value="KQJ83256"/>
        <property type="gene ID" value="BRADI_5g13960v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830788"/>
    <dbReference type="eggNOG" id="ENOG502QRP1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_92_6_1"/>
    <dbReference type="OMA" id="IIQQNIC"/>
    <dbReference type="OrthoDB" id="598358at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045088">
        <property type="term" value="P:regulation of innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000609">
        <property type="entry name" value="Inactive LRR receptor-like serine/threonine-protein kinase BIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000428">
        <property type="entry name" value="Inactive LRR receptor-like serine/threonine-protein kinase BIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000415">
        <property type="entry name" value="Inactive LRR receptor-like serine/threonine-protein kinase BIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF86">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095623" description="Protein kinase domain-containing protein" evidence="13">
        <location>
            <begin position="24"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="324"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQJ83256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="630" mass="67558" checksum="2B21CDFE88F6258B" modified="2012-06-13" version="1">MPPSLLPLLLLLALALPFPPAAPQPAPGSPSEPREDDLRCLRGVKDGLQDPDGRLASWNFRNTSAGALCDYSGVTCWNPQESRVITLSLSGFGLRGHVPSSLQYCGSANTFDLSGNGLDGPIPPALCDWIPFVVSLDLSGNKLSGPLPAELANCRFLNSLKLSDNAFTGQIPASLARLDRLKSLDLSLNQLEGQIPAQLGATFSKESFSGNSGLCGRPVSSRCGRGLGGAGLGIVIAAGVFGAAASLLLAFFFWRCTGKSKGGRRRRQSRGVSESGVTAAEDGSWWAERLRAAHNRLAPVSLFQKPIVKVKLADLLAATQDFSTGHIVVAGSSRAGTAYRAVLRDGSALTVKRLHSCPLSEKAFRSEMGRIGQLRHPNIVPLLGFCVVEDERLLVYKHMESGALCSVRKEPGETLLDWSTRLRIAVGASRGLAWLHHGFQMPQIHQNLSSSAVLLDEDYEARITDVGLTRLVRMAPGEGGDTSPFLNGDFGEFGYVAPEYASNPVGTMKGDTYAFGVILLELVSGQEAATVASDVAGEGFKGTLVDWVYQLKSSGRITDAVDKSLNGKGHDTEIGEFLKVAFQCISARPKERVSMYRVYHALKSIGTGRDVSEQFDEFPLSYNKDDSDTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1J3K8</accession>
    <name>I1J3K8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC100824553</name>
        <name evidence="24" type="ORF">BRADI_5g26820v3</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">Bd21</strain>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function as sodium-coupled metabolite transporter across the chloroplast envelope.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. KEA (TC 2.A.37.1) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579512.1">
        <property type="nucleotide sequence ID" value="XM_003579464.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240639.1">
        <property type="nucleotide sequence ID" value="XM_010242337.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J3K8"/>
    <dbReference type="EnsemblPlants" id="KQJ85402">
        <property type="protein sequence ID" value="KQJ85402"/>
        <property type="gene ID" value="BRADI_5g26820v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824553"/>
    <dbReference type="Gramene" id="KQJ85402">
        <property type="protein sequence ID" value="KQJ85402"/>
        <property type="gene ID" value="BRADI_5g26820v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824553"/>
    <dbReference type="eggNOG" id="KOG1650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_3_0_1"/>
    <dbReference type="OMA" id="YGFSRMM"/>
    <dbReference type="OrthoDB" id="4834at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140899">
        <property type="term" value="P:plastid gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042794">
        <property type="term" value="P:plastid rRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080022">
        <property type="term" value="P:primary root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001057">
        <property type="term" value="P:reactive nitrogen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900069">
        <property type="term" value="P:regulation of cellular hyperosmotic salinity response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010109">
        <property type="term" value="P:regulation of photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000377">
        <property type="term" value="P:regulation of reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000070">
        <property type="term" value="P:regulation of response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900140">
        <property type="term" value="P:regulation of seedling development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009646">
        <property type="term" value="P:response to absence of light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009744">
        <property type="term" value="P:response to sucrose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000007">
        <property type="entry name" value="K(+) efflux antiporter 2 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000134">
        <property type="entry name" value="K(+) efflux antiporter 2 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004771">
        <property type="entry name" value="K/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00932">
        <property type="entry name" value="2a37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46157:SF2">
        <property type="entry name" value="K(+) EFFLUX ANTIPORTER 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46157">
        <property type="entry name" value="K(+) EFFLUX ANTIPORTER 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="15 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1001">Plastid inner membrane</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="587"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="642"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="671"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="704"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="747"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="787"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="828"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="859"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="887"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="922"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="23">
        <location>
            <begin position="967"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="402"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1139"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="246"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KQJ85402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1163" mass="123709" checksum="BC92AAD6FF59B3EB" modified="2012-06-13" version="1">MDFSRFAAASGAAAPRPGLQIGAAAGNGSFRACSLRRARRRGTGLGCGGNLMVASALRGCGSSGLLLYAAPLKHHGTCCSRPPGRGIKCQGNDSLAYVDGPLEGTKGSSEGADEPAGGTSGSDEETRAAGVDVDDLRELLHRARKELEVARLNSTMFEGKAQRISESAIALKDKADGAQRDVSAAVATVQEIIGREEDAKEAVRKATMALSMAEARLQLAAEALDAKRGSVGQLEVSLDDVEEEAFASAQEEIKGCQASLSKCEEELSRIQGKKMELQKEVDRLTELAERALLDASKAEEDVSNIMVLAEQAVALEMEAAKRANDAELVLQKAEKAISSVDSVVELVPAAEGQISDEEDDLSDVYDYSGDGIADISERDEVSNVERLMQIGDLAAEGIEQLESSHEMSNDERSDKLLVEPQKEAESDTDKSKQGKKPDIERKESKSSLKRSSRFFSASFFSSKSDGEFTPTSVFRGLMKSARKQAPKLVVGTLLLGAGVFFFSRAQKSNQLFQQPGIATSIEEVSSTAEPIVREIRQIPKRIKKLIELLPHQEVNEEEASLFDILYLLLASVVFVPLFQKIPGGSPVLGYLAAGVLIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTTAVVGMVAHRFAALPGPAAIVIGSGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGVGFQAIAEAMGMAAVKAIAAITAIIAGGRLFLRPIYRQIAENRNAEIFSANTLLVIFGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLFMSNYPAISLILGLLIIGKTLLVTFIGRLFGISTIAAVRVGLLLAPGGEFAFVAFGEAVNQGLLSPQLSSLLFLVVGISMAMTPYLAAGGQFLASKFEQHDVRSLLPVESETDDLQGHIIILGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGADRACAAAITLDTPGANYRAVWALSKYFPNVKTFVRAHDVDHGVNLEKAGASAVVPETLEPSLQLAAAVLAQAKLPMSEIATTINEFRNRHLSELTELCTTTGSSLGYGFSKVMPKTKPPTSDDDGESVDGALAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E5N9</accession>
    <name>A0A0Q3E5N9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g01208v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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                <consortium name="EnsemblPlants"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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    </evidence>
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        <source>
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            <strain evidence="1 2">Bd21</strain>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Bevan M.W."/>
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    </evidence>
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        <source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="1">Bd21</strain>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
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        <source>
            <dbReference type="EMBL" id="KQJ96614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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            <taxon>Poaceae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317245.1">
        <property type="nucleotide sequence ID" value="XM_024461477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FPQ6"/>
    <dbReference type="STRING" id="15368.A0A0Q3FPQ6"/>
    <dbReference type="EnsemblPlants" id="KQK01400">
        <property type="protein sequence ID" value="KQK01400"/>
        <property type="gene ID" value="BRADI_3g55602v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112271707"/>
    <dbReference type="Gramene" id="KQK01400">
        <property type="protein sequence ID" value="KQK01400"/>
        <property type="gene ID" value="BRADI_3g55602v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1705400at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055298">
        <property type="entry name" value="AtLOH3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006580">
        <property type="entry name" value="Znf_TTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 2-RELATED ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11697:SF230">
        <property type="entry name" value="ZINC FINGER, MYM DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
        <property type="entry name" value="ZnF_TTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTF-type" evidence="2">
        <location>
            <begin position="135"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK01400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="846" mass="95906" checksum="17D957B72CE45D03" modified="2016-01-20" version="1">MKKRSVSSGDRSTGDLRRYLTKKSSAPPTDDGADETERTIPNPPRNESGPIPPISESTNPATNVSTNSTVDESTHRPKRLRLEFDPNHIIPDPALRIPIEDYANEIRDEVRRAYLLKGRTEANGLVFEQTLDGKVWRSFQRSWLGKYDWLEYSVKENAAFCFYCFLFKKTAQPNTFGNDVFSRDGYSRWKTATAAFKKHVGGPNSFHNIARGKCEDFINQRSSVSSKIHAYSKESEIAYKIRLTASLDCARYLIAQGEAFRGHDESSTSINKGNFKELLEWYKDKNSEVKEAFEKGPGNALMVCSDIQKQLAKCCAQEVTSVIKSEIGDRNFAVLIDEARDSSIKEQMAVIVRFVNNQGKVMERFLAMKHISDCTSAALKEALLHLLALHDLPISRLRGQGYDGASNMRGEFNGLQKLIRDDSPHAFYVHCFAHQLQLVVVTIAKCSKCISDFFNHVPLIVTQVSASCKRKDTLIAKHQDVLLEKLENGKISSGKGLHQESGLARPGDTRWGSHLKTLLRIYQMWESVVDVLTIVSTDAREHSSVGGAGGLVKKMECYEFVFILNFLIGLLTITNDLSQALQRKDQDIVEAMGLISDVKTRLQDVRDNGWEPLLDQVKSFCEEYGINVPNMDDLTVPMGESVRPKNKVTLRHYYKVDIFNVAIDATMTEINHRFNEVSSELLVCMSCLNPSNSFAMFNVDKLVRLAEIYEADFTISDRLMLRSQLQIFILNIRRSEKFHGCSDISKLSQLMVETKKDKTFPLVYRLIELTLILPVATASVERIFSAMSIIKTDLRNKLSDGWLNDLMVCYTEREIFKSIDDAKITKRFQSMKDRRGHLPKERNVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FVI3</accession>
    <name>A0A0Q3FVI3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BRADI_2g07730v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FVI3"/>
    <dbReference type="FunCoup" id="A0A0Q3FVI3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK03416">
        <property type="protein sequence ID" value="KQK03416"/>
        <property type="gene ID" value="BRADI_2g07730v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03416">
        <property type="protein sequence ID" value="KQK03416"/>
        <property type="gene ID" value="BRADI_2g07730v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FVI3"/>
    <dbReference type="OrthoDB" id="640735at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FVI3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036297493" description="Subtilisin-like protease" evidence="11">
        <location>
            <begin position="20"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="13">
        <location>
            <begin position="45"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="146"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="14">
        <location>
            <begin position="637"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK03416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="737" mass="76798" checksum="616CBAF12832965C" modified="2016-01-20" version="1">MVSFTSILLPLLLLATLHSTPTLCYVNPASPVVQKDDTKTSAGRTYIVLVEPPRLADQYAHRRWHESFLPSPCADVSGKPCLLHSYTEAFSGFAARLTDVELDAVAKKPGFVRAFPDRMLQPMTTHTPEFLGLRTGTGFWTDAGYGKGVIVGLLDTGIYAKHPSFDDHGVPPPPARWKGSCKAERCNNKLIGAMSFTGDDNSDDDEGHGTHTSSTAAGNFVAGASSHAVSAGTAAGIAPGAHIAMYKVCNSLGCTESAVLAGLDKAVKDGVDVLSMSLGGGSSFRFDQDPIAMATFRAASKGVIVVCSAGNNGPTPGSVTNDAPWLLTVAAGSVDRSFDAAVHLGNGKIIEGQALNQVVKPSSELYPLLYSEERRQCSYAGESSVVGKMVVCEFVLGQESEIRGIIGAGAAGVVLFNNEAIDYATVLADYNSTVVQVTAADGAVLTNYARSTSSSKAALSYNNTVLGIRPAPIVASFSSRGPSRSGPGVLKPDILAPGLNILAAWPPRTDGGYGPFNVLSGTSMSTPHVSGVAALIKSVHPGWSPAAIKSAIVTTADAVNSTGGSILDEQHRKANVFAAGAGHVNPARAADPGLVYDIHADEYVGYLCWLIGNAGPATIVGNSRLPCKTSPKVSDLQLNYPTITVPVASSPFTVNRTVTNVGPARSTYTVKVDAPKSLAVRVFPETLVFSKAGEKKTFSVSVGAHGVQADELFLEASLSWVSGKHVVRSPIVASARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FZB1</accession>
    <name>A0A0Q3FZB1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100836630</name>
        <name evidence="2" type="ORF">BRADI_2g15400v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT70661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565853.2">
        <property type="nucleotide sequence ID" value="XM_003565805.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FZB1"/>
    <dbReference type="STRING" id="15368.A0A0Q3FZB1"/>
    <dbReference type="EnsemblPlants" id="KQK04704">
        <property type="protein sequence ID" value="KQK04704"/>
        <property type="gene ID" value="BRADI_2g15400v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT70661">
        <property type="protein sequence ID" value="PNT70661"/>
        <property type="gene ID" value="BRADI_2g15400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836630"/>
    <dbReference type="Gramene" id="KQK04704">
        <property type="protein sequence ID" value="KQK04704"/>
        <property type="gene ID" value="BRADI_2g15400v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT70661">
        <property type="protein sequence ID" value="PNT70661"/>
        <property type="gene ID" value="BRADI_2g15400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836630"/>
    <dbReference type="OrthoDB" id="605328at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FZB1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055290">
        <property type="entry name" value="At3g26010-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006527">
        <property type="entry name" value="F-box-assoc_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35546">
        <property type="entry name" value="F-BOX PROTEIN INTERACTION DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35546:SF105">
        <property type="entry name" value="OS05G0139200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07734">
        <property type="entry name" value="FBA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK04704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="369" mass="42224" checksum="841EB0D212B8F3D4" modified="2016-01-20" version="1">MAAMERAQRCSAASLTEDLIVEILSRVPVKPLCRFKCVSKAWENLISHPEHRKKLPQTLAGFLYTSLSGLLPALAFTNASPQEERYPIFPSNFSFLPEFQDIRIMDCCNGLLLCRLWESTDDFHYVLCNPATEKFTTVPPSGNADNLYAARLGFDPAVSSSRFHVFELVEYEEDDPVITGVRVYSSETREWVHKEVEWSDDALLESRLSRTVFLNGSLHILTDQPAVLTVDTELKTWRKIHLSLLQDQDGFIQQSQGRLHYVNFREFDSVTRLAVYILEDYGGDDWVLKHSVEASRIFGTTDLQLVDWVAMHPECNLLFFSVGWDGTLMSYDMDRQQVRVIRNLGHDSLPPYLPCVPFYSELPALENET</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GAB9</accession>
    <name>A0A0Q3GAB9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g41294v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GAB9"/>
    <dbReference type="STRING" id="15368.A0A0Q3GAB9"/>
    <dbReference type="EnsemblPlants" id="KQK08344">
        <property type="protein sequence ID" value="KQK08344"/>
        <property type="gene ID" value="BRADI_2g41294v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08344">
        <property type="protein sequence ID" value="KQK08344"/>
        <property type="gene ID" value="BRADI_2g41294v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GAB9"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK08344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="100" mass="11270" checksum="05955C46BC8096D8" modified="2016-01-20" version="1">MASLCSNPHIIECKDGWVDERTSACIFTSCCEGGEIAARSRRQGASYFLKNYLHCNRVLRHHFKCSNILLTKDRQTASKVVEAKEAALCRCLNILNSLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GMC7</accession>
    <name>A0A0Q3GMC7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_2g61190v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRT-like transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GMC7"/>
    <dbReference type="FunCoup" id="A0A0Q3GMC7">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK11613">
        <property type="protein sequence ID" value="KQK11613"/>
        <property type="gene ID" value="BRADI_2g61190v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT73615">
        <property type="protein sequence ID" value="PNT73615"/>
        <property type="gene ID" value="BRADI_2g61190v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11613">
        <property type="protein sequence ID" value="KQK11613"/>
        <property type="gene ID" value="BRADI_2g61190v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73615">
        <property type="protein sequence ID" value="PNT73615"/>
        <property type="gene ID" value="BRADI_2g61190v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GMC7"/>
    <dbReference type="OrthoDB" id="416555at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GMC7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034635">
        <property type="term" value="P:glutathione transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013936">
        <property type="entry name" value="CRT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31326">
        <property type="entry name" value="PROTEIN CLT2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31326:SF3">
        <property type="entry name" value="PROTEIN CLT3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08627">
        <property type="entry name" value="CRT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK11613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="374" mass="39296" checksum="0096CD1F93EA3397" modified="2016-01-20" version="1">MRMRRSGRGEKAAAACAAAGQVAAGGAGTGSRSVGMEVALATAAVVVMGTGNRVLYKLALVPLRQYPFFLAQFATFGYVVVYFSVLFLRYQAGAITDEMLSLPQKPFILVGLLEALAAAAGMAAGAVLSGASIPILSQTYLVWQLLLSAIFLKRRYRVNEITGCFLVTVGVVITVASGSGAGASLQSTGILWPLLMIISFFLQAADTVLKEIIFIDAAKNLKGGSVDLFVVNSYGSAYQALFMFLLLPILSKLWGIPFHLLPTYIRDGAACFLNMGSLSSGCEGAPLLPLLFVLVNMGFNISLLHLLKISSAVVSCLASTFSVPLSIYAFTLPLPYIGVASSLPPGFVAGAAVLIAGLLLYCLPQAQNSGNRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GS39</accession>
    <name>A0A0Q3GS39_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_1g08670v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GS39"/>
    <dbReference type="FunCoup" id="A0A0Q3GS39">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK13221">
        <property type="protein sequence ID" value="KQK13221"/>
        <property type="gene ID" value="BRADI_1g08670v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13221">
        <property type="protein sequence ID" value="KQK13221"/>
        <property type="gene ID" value="BRADI_1g08670v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GS39"/>
    <dbReference type="OrthoDB" id="680539at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999575" description="Peptidase S8/S53 domain-containing protein" evidence="8">
        <location>
            <begin position="25"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="46"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="143"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQK13221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="65353" checksum="D3809BF7F65A395B" modified="2016-01-20" version="1">MASRTFIFLQYSLLFLTLLQSTHSAITLKPKNTIEQKTQTPSTSNTYIIQTNHLSKPSRFTTLDHWYASMVATNSTGRILHTYDTVMHGFAVRLTDAEARRMSRIPGVFGVHRDRVYHTQTTRSPGFIGLHADFGAWPDSEFGDNVIIGVVDTGISPASSSFNDSGLGPVRPGWKGKCVDAEGFSASSCNRKLVGDKVFINEEDGIFTPRDKLGHGTHVASTAAGSKVQGANMLGFSQGSASGVARMARIAMYKACNTLTCTESAIVAAIDAAVSDGVDIISLSLGKLQDPAFYDDMVAVARFGATRRGVFVVFAGGNSGPIASTVSNVAPWMTTVGAATTDRVFPGRLRLGNGVETDLAPDKVMGKIVVCTGMEGASNGFYVQRAGGAGVVSVDSDALFSDGVMVEAYSLPGLTLSTTGRKKLDAYMVSAVPFSSDTVTGETRAPMVAGFSSRGPNPLAPELLKPDVVAPGVNILAAWPGDLSPTNNGIDPRRVEYNIMSGTSMACPHVAGVAALIRKRHGDWTPAMVRSAMMTTADTLDMNGRGIIDNGSGNDTTPLEAGAGLVLPLLAMDPGLVYDASANDYGAFLCSLNYTVEQVRRFVPDLASCPSSGDRREGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GTC1</accession>
    <name>A0A0Q3GTC1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BRADI_1g11330v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13613.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GTC1"/>
    <dbReference type="STRING" id="15368.A0A0Q3GTC1"/>
    <dbReference type="EnsemblPlants" id="KQK13613">
        <property type="protein sequence ID" value="KQK13613"/>
        <property type="gene ID" value="BRADI_1g11330v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK13613">
        <property type="protein sequence ID" value="KQK13613"/>
        <property type="gene ID" value="BRADI_1g11330v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GTC1"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000126">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF21">
        <property type="entry name" value="OS06G0642300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035999592" description="Cytochrome P450" evidence="12">
        <location>
            <begin position="18"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK13613.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="52194" checksum="AE0E019A26AA760A" modified="2018-03-28" version="1">MRYALCFLVALFVYAAARCVVLNVTQQPRPRLPPGPWQLPLIGSLHHLRRGLPHRAMRDLSLRHGPLMLLRVCERAVIVVSSAQAVRDICKGYDAAFSERPSSPGIEELSRHGRGIIFAPYGDHWRLLRRIYPHDGAAQPPPRRGAAAAGGRRRLVNIDERLGEFMTGAAVRAIFGDRMPDGAAFLKMVKQGADPSSLFDLRDLFPSSRLVRLLPRSRGKAERHRQNMFRLMDNILESHEERRAARDGDDLYDMVDDVFGAALDTTTTTLQWAMSELMANPRVMQKSQLETRRIFTRHDKVHDTTLNGLQYLKAAIKETLQLHPPATLLPRQRLQACKIQGYDVPTVVVVVINIWAISRDPKYWDEPEKFMPERFEGDNGNVADFKGADFEFTPFGVGRRICPGIGFAYANVEIALANLLYHFDWELPVGVAPQEIDMTEVFGVTVSRKTELYLCPIPHSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GV75</accession>
    <name>A0A0Q3GV75_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BLE2 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100833441</name>
        <name evidence="3" type="ORF">BRADI_1g19400v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559874.3">
        <property type="nucleotide sequence ID" value="XM_003559826.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GV75"/>
    <dbReference type="FunCoup" id="A0A0Q3GV75">
        <property type="interactions" value="739"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GV75"/>
    <dbReference type="EnsemblPlants" id="KQK14904">
        <property type="protein sequence ID" value="KQK14904"/>
        <property type="gene ID" value="BRADI_1g19400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833441"/>
    <dbReference type="Gramene" id="KQK14904">
        <property type="protein sequence ID" value="KQK14904"/>
        <property type="gene ID" value="BRADI_1g19400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833441"/>
    <dbReference type="OrthoDB" id="618849at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33115">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33115:SF11">
        <property type="entry name" value="OS07G0654700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK14904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="944" mass="106181" checksum="89A08BC6FEED88F1" modified="2016-01-20" version="1">MEDVAEHRVHMPAADAGKPPNSKSPEKWLNGFVRAVALMEKVGNALGTLAFTWATVVLLGGYTKDLEDDFWFATAIVFLEAARMFTRKNRMEYRLVFNNRGAFRPFGSNGLIMMVCFLNLSVILLREVEPTILVISGVLAMTTLVIGWFLSRSGLKLPICHPLRRATSLWSPVVGILLLVPSLSYHSRGYLYGHDQRQYIFAKFLVFTSLLAAVLLVSISRLRFPSIVKLVDRSLGSRQVFWRRVILYFCMVGALITHLCMLGPHGRVLIIIILACALAVVSFGNFQVPAAVLRVVLALLHFELFKSNTMYDDNLKASLAIFYGVVLGQGTLYSVACTVEFFSFIPRRSLVHCGGFRGQYGVESVNLYYAYAFEKCMQDGVFAPTKTSLGTFAMDSVNSDSSKNQLYGLQMMHNFLQKEPTKAPLLYKLTTSPKTMARIIRMLDWTSPKDAIIRLYAAKVTSEFAKNLRVVAFPGTMQIVSALLAADSRPKIGNPLLDTDDEQEDSQDEEHDPSKDAADNQLQRQDQLPDTDNLLQTQTCPTQQVSINPQNSMLRCWQRISEFWSIPKEMPLTNNDLLPALAMSIIDSLAGCDQDNCAEISKAADLIQKIVEFTGNRSDEMITEAQQKILLLSSLKVMQRLTSIGGKIGITLRYEIAKDPFLQRNLKEILGDSMTSPEFKRLVAAILRNLAVDGDTRQEISRIQLIITMLMQTFINTEGTMSNNAGRLLRKVAGQALAMLTIESVHTCLIMLKEPEFIKELKTMILIHDDKYMYVAASLLRNLCLHAQSELRELDLKEISHTLREVLERIMDTERAELEILVGLSSQICKVIPGDFTRELDDGHTKQRFVKRLVVALIANKKPRAHCPGIRRLILEQVIFMTECNSNYADCFNECRMKEALLMVEQTLSKVENYRLFLGNTGFMEYSMPISALVARAKELLGCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GY64</accession>
    <name>A0A0Q3GY64_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100841285</name>
        <name evidence="7" type="ORF">BRADI_4g01693v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ85786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579138.1">
        <property type="nucleotide sequence ID" value="XM_003579090.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GY64"/>
    <dbReference type="FunCoup" id="A0A0Q3GY64">
        <property type="interactions" value="324"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GY64"/>
    <dbReference type="EnsemblPlants" id="KQJ85786">
        <property type="protein sequence ID" value="KQJ85786"/>
        <property type="gene ID" value="BRADI_4g01693v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841285"/>
    <dbReference type="Gramene" id="KQJ85786">
        <property type="protein sequence ID" value="KQJ85786"/>
        <property type="gene ID" value="BRADI_4g01693v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841285"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045543">
        <property type="term" value="F:gibberellin 2-beta-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045487">
        <property type="term" value="P:gibberellin catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="171"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ85786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="341" mass="36964" checksum="EDBF0FEFC9671FEF" modified="2016-01-20" version="1">MEQRRAESPIPADGAAASKLEELELPTLDLEEESGLTEALAAACRDLGVFRLANHGIPADLSDRLFSLTRHLLEEPDAEKKAKLPGYFSGTPALSLRVKELNWVEGFHVAPAAADADPDPTAADDDIGGEFGSFREVTAEYVSRMARIARKLFDAMDLGLDASQRASYLAEHDGTFRAYRYPACPAGGHIGMEAHTDSSVLSILNQQDLQESSLQVLLRPQGTWRSVQPVEGTLVVNIGDMMQAITGDAYRSVEHRVVPLPDTDRMSLCYFAFPQDDAVISGCGEDDGEKGSCYRPFSYREFREQVQADIKATGAKVGLARFRRGASAPELASAIGTEAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H1V4</accession>
    <name>A0A0Q3H1V4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100826815</name>
        <name evidence="9" type="ORF">BRADI_1g31391v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16912.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560391.1">
        <property type="nucleotide sequence ID" value="XM_003560343.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H1V4"/>
    <dbReference type="FunCoup" id="A0A0Q3H1V4">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3H1V4"/>
    <dbReference type="EnsemblPlants" id="KQK16912">
        <property type="protein sequence ID" value="KQK16912"/>
        <property type="gene ID" value="BRADI_1g31391v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826815"/>
    <dbReference type="Gramene" id="KQK16912">
        <property type="protein sequence ID" value="KQK16912"/>
        <property type="gene ID" value="BRADI_1g31391v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826815"/>
    <dbReference type="OrthoDB" id="514967at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H1V4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF114">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="8">
        <location>
            <begin position="167"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="234"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="383"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK16912.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="416" mass="44744" checksum="5243992DF32277DF" modified="2018-03-28" version="2">MMSGRMNNSTGSDDFPFAPTPPPSYGGFEQRALYDSFDFAAAFQFQHQQEHHQMLSLPPNANTSNLLHHPMAPPPPPAAMSMQLPIPMPQMHGHGGDAMIYPALGMAVKREGEVAEGRNIGLNLGRRTYFSPGDMMAVDRLLMRSRLGGVFGLGFGGPGGHGHHHQPPRCQAEGCKADLSGAKHYHRRHKVCEYHAKASLVSAGGKHQRFCQQCSRFHVLTEFDEAKRSCRKRLAEHNRRRRKPATTNGTATSAAKDSATPPSSKKPNNGAGGAIIGSYTVDNKTLSAAKSSTISSNTSGISCLQQQQQDQSKAAAAAALTLGGSPQDQSNAVHQLAGHGHGHHHQEQHFITSLLHNNNNNNNGNNNNILSCSSVCSNAMPPPATANNGGGEVSDQNNHHGNNNSNNMHLFEVDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HCL2</accession>
    <name>A0A0Q3HCL2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF506 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100832408</name>
        <name evidence="2" type="ORF">BRADI_1g55480v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228288.1">
        <property type="nucleotide sequence ID" value="XM_010229986.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HCL2"/>
    <dbReference type="STRING" id="15368.A0A0Q3HCL2"/>
    <dbReference type="EnsemblPlants" id="KQK20584">
        <property type="protein sequence ID" value="KQK20584"/>
        <property type="gene ID" value="BRADI_1g55480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832408"/>
    <dbReference type="Gramene" id="KQK20584">
        <property type="protein sequence ID" value="KQK20584"/>
        <property type="gene ID" value="BRADI_1g55480v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832408"/>
    <dbReference type="OrthoDB" id="691424at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HCL2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01615">
        <property type="entry name" value="A_thal_3542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF38">
        <property type="entry name" value="PLANT-SPECIFIC DOMAIN TIGR01615 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK20584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="40238" checksum="B75CEBB20D7CCA91" modified="2016-01-20" version="1">MEARMPRVSRLRRLLGRVSAAPERLAAGDRDREREEKSPAPEAEVGSAGLDRMVRSFMEDAATPAVERGPPPRAGRCGNCCFNGSAANSDGSDDEDEELVFDFLPSISGQSASSAAVGDTLEALKGLVQSTSLAERNLLADASRVAERCRGGGKQQQKKKGDLRLAVADGLRALGHDAAVCKSRWDKAPSFPAGEHEYIDAMVESEFELDAARLIVEVDFRAEFEVARPTKAYRAVLQALPAVFVGTPDRLGRIVAVVAEAARQSLRKKGLSFPPWRKPEYMRAKWLSPHARCSSGLDKQQQHPPSTPAATPVSAASFTGEFELRFDGETTKGHDSGGGGAQKITVVVSPWRPLEPEVTRKKRPVIAGLTSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HE14</accession>
    <name>A0A0Q3HE14_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g56685v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HE14"/>
    <dbReference type="EnsemblPlants" id="KQK20765">
        <property type="protein sequence ID" value="KQK20765"/>
        <property type="gene ID" value="BRADI_1g56685v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20765">
        <property type="protein sequence ID" value="KQK20765"/>
        <property type="gene ID" value="BRADI_1g56685v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HE14"/>
    <dbReference type="OrthoDB" id="1934998at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK20765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="142" mass="15295" checksum="16D4C3D5CE0F8D74" modified="2016-01-20" version="1">MVLKPLSGCDATHCSMYKFFLLLPLAPEAALVFHPLPPGEVVGGDALVLLPLPPGEVVGGAALVQHHPNLHLINSVSSNKSPMASSSSVNKRVHLKECMIVPVISWIITFLIWLQLVKLPPLRIPRSSPAAASKRDRDARTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HGX2</accession>
    <name>A0A0Q3HGX2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100833655</name>
        <name evidence="4" type="ORF">BRADI_1g62820v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561650.2">
        <property type="nucleotide sequence ID" value="XM_003561602.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HGX2"/>
    <dbReference type="STRING" id="15368.A0A0Q3HGX2"/>
    <dbReference type="EnsemblPlants" id="KQK21749">
        <property type="protein sequence ID" value="KQK21749"/>
        <property type="gene ID" value="BRADI_1g62820v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833655"/>
    <dbReference type="Gramene" id="KQK21749">
        <property type="protein sequence ID" value="KQK21749"/>
        <property type="gene ID" value="BRADI_1g62820v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833655"/>
    <dbReference type="OrthoDB" id="1875580at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HGX2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF66">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK21749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26147" checksum="AC8F63745C43545F" modified="2016-01-20" version="1">MRAGAGGVRMERPSDQPLHHVHGHGHGKKKPMSRIREDDEHKSSWWVWALVILCTLVAIGVIVAGATVFAVYLLYKPKMPYMVVTGAQLGRLVYGQDGVIHDLQVSINILARNTNSRADASFSRVNIAVGFHGADLARLQARPFRVARESETPLPYDVVSKGAGLDPAGMRSMDEALKRHVVPLDLFGKARTRWKMGIFLNLQFWTRISCRLHFNFPGNGTALPIDRDSCRSRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HH73</accession>
    <name>A0A0Q3HH73_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">COBRA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100831307</name>
        <name evidence="10" type="ORF">BRADI_1g64817v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COBRA family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753395.1">
        <property type="nucleotide sequence ID" value="XM_014897909.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HH73"/>
    <dbReference type="STRING" id="15368.A0A0Q3HH73"/>
    <dbReference type="EnsemblPlants" id="KQK22056">
        <property type="protein sequence ID" value="KQK22056"/>
        <property type="gene ID" value="BRADI_1g64817v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831307"/>
    <dbReference type="Gramene" id="KQK22056">
        <property type="protein sequence ID" value="KQK22056"/>
        <property type="gene ID" value="BRADI_1g64817v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831307"/>
    <dbReference type="OrthoDB" id="2014623at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HH73">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010215">
        <property type="term" value="P:cellulose microfibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056900">
        <property type="entry name" value="COB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006918">
        <property type="entry name" value="COBRA_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052">
        <property type="entry name" value="COBRA-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31052:SF3">
        <property type="entry name" value="COBRA-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25079">
        <property type="entry name" value="COB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04833">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044545958" description="COBRA C-terminal domain-containing protein" evidence="8">
        <location>
            <begin position="27"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="646"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="COBRA C-terminal" evidence="9">
        <location>
            <begin position="432"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK22056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="670" mass="71683" checksum="74AEBFC75733DDB3" modified="2016-01-20" version="1">MAGALAPPHVGLALLGVLLLAGLAAAQTPRTPAAPAPAPDPGCNGIELTYNFQGRTMIRPFVGDKNRQPYSFKANASVLNSGTRPLKSWALLVTFGHDEILVGVDGAVLTGGADLPYNTTENAGNATSFSGYPQTDLLTPIGTAGDIAQIQASVGLVGTLFAGPNFVPLPTALSLDDPAYNCPAATNLTAGVLTTCCVLTPEAEANATVIDANATDPTKNFLPRGTGDLVITYDVVQAYGSSYLALVTLENNAKLGRLDNWRLSWEWRRGEFIHSMKGAHPLVVDTSACINGPAGQYYQSLDFSQVLNCEKKPVILDLPLSRYNDTNMGKIEHCCRNGTILPKSMDEAQSKSAFQMQVFKMPPDTNRTKLFPPANFKISGGSSLNPDYSCGQPVPVSPTAFPNPSGLDSTTLAIATWQVVCNITTAKGAKPKCCVTFSAHYNDSVIPCNTCACGCPANRRGPMCSTSAQSMLLPPEALLVPFDNRTQKAQAWAELKHYNVPRPMPCGDFCGVSINWHVSTDFNKGWSARVTLFNWGDVDMANWFAAMVMDKAYDGFEKAYSFNATAQGNNTIFMQGLEGLNYLVKQTNMSGSQYLVPGKQQSVLSFTKSQTPEINVVAGDGFPSKVFFNGDECAMPQRLPMRSGGFGTHLSSALALILFLAASTFVLLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HID9</accession>
    <name>A0A0Q3HID9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_1g68945v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HID9"/>
    <dbReference type="STRING" id="15368.A0A0Q3HID9"/>
    <dbReference type="EnsemblPlants" id="KQK22711">
        <property type="protein sequence ID" value="KQK22711"/>
        <property type="gene ID" value="BRADI_1g68945v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22711">
        <property type="protein sequence ID" value="KQK22711"/>
        <property type="gene ID" value="BRADI_1g68945v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HID9"/>
    <dbReference type="OrthoDB" id="1721314at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052101">
        <property type="entry name" value="Plant_StressResp_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983:SF7">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983">
        <property type="entry name" value="PTO-INTERACTING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK22711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15173" checksum="59EC5E0822F5B58F" modified="2016-01-20" version="1">MSNYSEADFSSQLSMVSRLKNEYFIQLMATTWMRATGPRTRRYAMTGQLNQKSNVYSFGVIFLDATPRLGEDKVKQCIDPKLGNNDYPPKAVAKVMMQVGQCLQYIRRFCSLWSCILLSLSPDCLLLLFFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I7K4</accession>
    <name>A0A0Q3I7K4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA N(3)-methylcytidine methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100844642</name>
        <name evidence="5" type="ORF">BRADI_5g05210v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent methyltransferase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the methyltransferase superfamily. METL family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581102.1">
        <property type="nucleotide sequence ID" value="XM_003581054.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I7K4"/>
    <dbReference type="FunCoup" id="A0A0Q3I7K4">
        <property type="interactions" value="2438"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3I7K4"/>
    <dbReference type="EnsemblPlants" id="KQJ82056">
        <property type="protein sequence ID" value="KQJ82056"/>
        <property type="gene ID" value="BRADI_5g05210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844642"/>
    <dbReference type="Gramene" id="KQJ82056">
        <property type="protein sequence ID" value="KQJ82056"/>
        <property type="gene ID" value="BRADI_5g05210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844642"/>
    <dbReference type="OrthoDB" id="417697at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3I7K4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026113">
        <property type="entry name" value="METTL2/6/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809:SF14">
        <property type="entry name" value="TRNA N(3)-METHYLCYTIDINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037755">
        <property type="entry name" value="Mettl2_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR037755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ82056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="321" mass="36730" checksum="430865D79587BC33" modified="2016-01-20" version="1">MEGGGERDHEAEPEYHCHDFEWEDLRADVEANPSFSHHLSPFPTTAASPSPSSEAWRSFHRRHASGRFFKERRYLLKEFPDLLNNNDVAKMLEVGCGNGSTVVPILRCSRNNIVYACDCSKDTLEKANEIVNNTEGLDGKDRFHPFLLDVSKETFPDWLFCKSCQMSNAKAVDLLLDSSEHNTRKEHPVLLKENQCCVGGIDAVTMIFTLSAIPFNLMSITIQRCVSVLKPGGLVLFRDYGLYDMTMLRFSPSQRVGFREYMRADGTFSYFFTLDTMRELFHAAGLLELELEYCCVRSINRKNGKSMQRVWVHGKFQKPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IKJ6</accession>
    <name>A0A0Q3IKJ6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g25875v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IKJ6"/>
    <dbReference type="EnsemblPlants" id="KQK06347">
        <property type="protein sequence ID" value="KQK06347"/>
        <property type="gene ID" value="BRADI_2g25875v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06347">
        <property type="protein sequence ID" value="KQK06347"/>
        <property type="gene ID" value="BRADI_2g25875v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IKJ6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="106" mass="10705" checksum="13DC88B670B69E6F" modified="2016-01-20" version="1">MAYGPRLDSGRCRLAAGRGRHEGSRRREGIWAGGGGGGGRRGEETAEWSKAREGASSFSCRGLSRTASGEGCSAAAGGGGGAQVDQRVARGGERLAGGRGKREGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IMD5</accession>
    <name>A0A0Q3IMD5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100838096</name>
        <name evidence="10" type="ORF">BRADI_3g55614v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 13">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570414.1">
        <property type="nucleotide sequence ID" value="XM_003570366.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235981.1">
        <property type="nucleotide sequence ID" value="XM_010237679.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235982.1">
        <property type="nucleotide sequence ID" value="XM_010237680.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IMD5"/>
    <dbReference type="STRING" id="15368.A0A0Q3IMD5"/>
    <dbReference type="EnsemblPlants" id="KQK01402">
        <property type="protein sequence ID" value="KQK01402"/>
        <property type="gene ID" value="BRADI_3g55614v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01403">
        <property type="protein sequence ID" value="KQK01403"/>
        <property type="gene ID" value="BRADI_3g55614v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01404">
        <property type="protein sequence ID" value="KQK01404"/>
        <property type="gene ID" value="BRADI_3g55614v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01405">
        <property type="protein sequence ID" value="KQK01405"/>
        <property type="gene ID" value="BRADI_3g55614v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838096"/>
    <dbReference type="Gramene" id="KQK01402">
        <property type="protein sequence ID" value="KQK01402"/>
        <property type="gene ID" value="BRADI_3g55614v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01403">
        <property type="protein sequence ID" value="KQK01403"/>
        <property type="gene ID" value="BRADI_3g55614v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01404">
        <property type="protein sequence ID" value="KQK01404"/>
        <property type="gene ID" value="BRADI_3g55614v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01405">
        <property type="protein sequence ID" value="KQK01405"/>
        <property type="gene ID" value="BRADI_3g55614v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838096"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IMD5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000026">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF495">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="118"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK01404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="316" mass="35657" checksum="A03AE832544448ED" modified="2016-01-20" version="1">MDPALLDNIIGRLLEVKSLKPGKNAQLSESEIKHLCAASKEIFLSQPNLLELEAPIKICGDVHGQYSDLLRLFDYGGYPPESNYLFLGDYVDRGKQSLETICLLLAYKVKYPENFFLLRGNHECASVNRIYGFYDECKRRFSVKLWKTFTDCFNCLPVSALIDEKILCMHGGLSPELNKLDQILNLNRPTDVPDTGLLCDLLWSDPSNEAQGWAMNDRGVSYTFGPDKVAEFLEKHDLDLICRAHQVVEDGYEFFADRQLVTIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPARKVLSGSTNTKSGFKSLRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3INL9</accession>
    <name>A0A0Q3INL9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g35547v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3INL9"/>
    <dbReference type="EnsemblPlants" id="KQK07451">
        <property type="protein sequence ID" value="KQK07451"/>
        <property type="gene ID" value="BRADI_2g35547v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK07451">
        <property type="protein sequence ID" value="KQK07451"/>
        <property type="gene ID" value="BRADI_2g35547v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3INL9"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK07451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="125" mass="12955" checksum="3AFB16DBA721AAD1" modified="2016-01-20" version="1">MFSSGAQLLASSLEALLSFLPFPHGPHPLFPSSFFSSPPALPVAISGRRSCSAASGPELGAARVRRASRGTTRGAARLAAFFSTATKGALRVFSAADIMNPNEDEAPGGALEEDKSADLIPQRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IU65</accession>
    <name>A0A0Q3IU65_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g10936v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IU65"/>
    <dbReference type="EnsemblPlants" id="KQK03972">
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="86" mass="10094" checksum="82CC22D12B9A8DED" modified="2016-01-20" version="1">MQLQNHCGAICLKDSAEERENVVVILFLLTKVTQPKYNYLLYKNYMLVMPNLLFSTMLDVMPLIPLFFVLFLLVFVKLYVLDEIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JAD7</accession>
    <name>A0A0Q3JAD7_BRADI</name>
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        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_2g47271v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DVL/RTFL small polypeptides family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JAD7"/>
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        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09308">
        <property type="protein sequence ID" value="KQK09308"/>
        <property type="gene ID" value="BRADI_2g47271v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09308">
        <property type="protein sequence ID" value="KQK09308"/>
        <property type="gene ID" value="BRADI_2g47271v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="1057178at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
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    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK09308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="40" mass="4916" checksum="84F64A1B87864DBE" modified="2016-01-20" version="1">MNKPRYQGKHREGLGRMLREHKARLYIIRRCVVMLICHHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JE22</accession>
    <name>A0A0Q3JE22_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g24676v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQJ96544"/>
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    </dbReference>
    <dbReference type="Gramene" id="KQJ96544">
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        <property type="gene ID" value="BRADI_3g24676v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ96544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="115" mass="12755" checksum="CF2C921CBF00595D" modified="2016-01-20" version="1">MGAFGGPRGGVRQENIPGSLLPKRSTDAAILEFSPLWRPGAPPPPPLPPATASDRRPRATPTPPPPSGDRERPQRLPPVTASDPDASLWRSRGSSPLRRSRIWYMVPYQTWRSWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JLB9</accession>
    <name>A0A0Q3JLB9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC104581572</name>
        <name evidence="15" type="ORF">BRADI_1g42458v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014752159.1">
        <property type="nucleotide sequence ID" value="XM_014896673.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JLB9"/>
    <dbReference type="EnsemblPlants" id="KQK18435">
        <property type="protein sequence ID" value="KQK18435"/>
        <property type="gene ID" value="BRADI_1g42458v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT75965">
        <property type="protein sequence ID" value="PNT75965"/>
        <property type="gene ID" value="BRADI_1g42458v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581572"/>
    <dbReference type="Gramene" id="KQK18435">
        <property type="protein sequence ID" value="KQK18435"/>
        <property type="gene ID" value="BRADI_1g42458v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75965">
        <property type="protein sequence ID" value="PNT75965"/>
        <property type="gene ID" value="BRADI_1g42458v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104581572"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JLB9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:immune response"/>
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        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Liguleless narrow1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF396">
        <property type="entry name" value="OS06G0496800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="96"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
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        <location>
            <begin position="387"/>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQK18435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="415" mass="46417" checksum="552F719D29420423" modified="2016-01-20" version="1">MGLPQNRLVSNRRLLGTSASPPAVGPSPSSPDGMKIMLAVLVVVIFCTLFYCVYCWRWRKRNAAVRRTQNEILRTISSSDLPLMDLALIHDATTNFSDANKLGEGGFGPVYRGVLPSGTEIAVKRLSTRSRQGTIEFENEVELIAKLQHRNLVRLMGWCSQHDEKLLVYEYLPNRSLDAFLFDTSKSAQLDWNTRHNIILGVARGLLYLHEDSLLKVVHRDLKASNVLLDNKMNPKISDFGLARIFRDGCNRVNTGRVVGTYGYMAPEFVMDGVFSVKSDVFGFGVLLLEILSGQRNGISYLEEHQQSLIQDAWKLWTDGRAKEIMDPTLGESYSREEAWGCFQVGLLCVQDDPDIRPTMSNVMLMLIGDHTSFPTPCKPLRNVPMSAPSSARKSESSLSLKSLNYASITAVQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K1P1</accession>
    <name>A0A0Q3K1P1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100823875</name>
        <name evidence="8" type="ORF">BRADI_2g14950v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04657.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567816.2">
        <property type="nucleotide sequence ID" value="XM_003567768.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K1P1"/>
    <dbReference type="STRING" id="15368.A0A0Q3K1P1"/>
    <dbReference type="EnsemblPlants" id="KQK04657">
        <property type="protein sequence ID" value="KQK04657"/>
        <property type="gene ID" value="BRADI_2g14950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823875"/>
    <dbReference type="Gramene" id="KQK04657">
        <property type="protein sequence ID" value="KQK04657"/>
        <property type="gene ID" value="BRADI_2g14950v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="24948at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K1P1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08366">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000079">
        <property type="entry name" value="Anaphase-promoting complex subunit 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016901">
        <property type="entry name" value="APC10/Doc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03256">
        <property type="entry name" value="ANAPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DOC" evidence="7">
        <location>
            <begin position="87"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="78"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK04657.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="273" mass="29830" checksum="43209B0E147B363A" modified="2018-03-28" version="1">MTCVHGRKPVTNFTSVGPAQFRRPQRALLGRTTLILGPALFIFPEPSPLLQSPNLFPVSPKSSTRAAPMESDGEEEAAAAAAATPGAGAAPPAGRLKGCPELSVDGDMREMAKTAAWSVSSCKPGNGVASLRDDSLDTYWQSDGAQPHLVNIQFQKKVQLQLVVLYVDFKLDESYTPSKISIRAGDGFHNLKEIKTVNLLKPVGWVHISLSGADPRETFIHTFMLQIAVLANHLNGRDTHIRQIKIYGPRPNPVPHQPFHFTSRECIMYSTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K2F3</accession>
    <name>A0A0Q3K2F3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g42594v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K2F3"/>
    <dbReference type="EnsemblPlants" id="KQK18460">
        <property type="protein sequence ID" value="KQK18460"/>
        <property type="gene ID" value="BRADI_1g42594v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18460">
        <property type="protein sequence ID" value="KQK18460"/>
        <property type="gene ID" value="BRADI_1g42594v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K2F3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <source>
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    </evidence>
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        <source>
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        <source>
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</entry>
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                <person name="Harmon-Smith M."/>
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                <person name="Shu S."/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    </evidence>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Pitluck S."/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">Bd21</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0A0Q3PBG1"/>
    <dbReference type="FunCoup" id="A0A0Q3PBG1">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3PBG1"/>
    <dbReference type="EnsemblPlants" id="KQJ86393">
        <property type="protein sequence ID" value="KQJ86393"/>
        <property type="gene ID" value="BRADI_4g05150v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ86393">
        <property type="protein sequence ID" value="KQJ86393"/>
        <property type="gene ID" value="BRADI_4g05150v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3PBG1"/>
    <dbReference type="OrthoDB" id="692283at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3PBG1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15251">
        <property type="entry name" value="TESTIS-SPECIFIC BASIC PROTEIN Y 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15251:SF2">
        <property type="entry name" value="TESTIS-SPECIFIC BASIC PROTEIN Y 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="486"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ86393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="521" mass="55640" checksum="7B16CE4727F968DC" modified="2016-01-20" version="1">MIRESWVVHAIRPPALGIRHTAPKTSQPSPPSSLPQEHEKEASESPLPSQLPPLNCESIIAGAPLCSFNCPAVFVMEVGGSSAGSSTRSGEGDLCAAQESREEEKARSGGGAMRARVCARVRTPHGVGALLLVGGAIVGAAVVAWRRRGDGKGANHHQHREKQPAKEEGFLDGGVVEDEQIQGKFDQSDENSSREDTEVGTNGLDGKETHDLHENQEEEKKEEEINADKLDSKLVEKFDPNSSRGLAEIVTVDTVSSAFCTNKESGDVNKADQNSRKNDIEEEVMPNAIEGVESVENCDQSTLTISSLEIAREEHNDGPQETASTQATAPQLLISEEAKADTMAETAEVELAEGTAIEENVSEHEAAEAELSKGTTVEENVSEQEAAEEELAKGTTMEENVSEHEEHKQAAEKPAVAAVIDSSVISSPQYLLKSAEKKRPANPEWKESGMKLYQDQDCGNGELSKVGTKQGGVAVVTVDRRAASMAVLAIILAVTIGVNAIFAVTIGVNIISCLYSHLRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PS50</accession>
    <name>A0A0Q3PS50_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100839745</name>
        <name evidence="5" type="ORF">BRADI_4g42997v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311125.1">
        <property type="nucleotide sequence ID" value="XM_024455357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PS50"/>
    <dbReference type="EnsemblPlants" id="KQJ92337">
        <property type="protein sequence ID" value="KQJ92337"/>
        <property type="gene ID" value="BRADI_4g42997v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839745"/>
    <dbReference type="Gramene" id="KQJ92337">
        <property type="protein sequence ID" value="KQJ92337"/>
        <property type="gene ID" value="BRADI_4g42997v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3PS50">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1534">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ92337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="547" mass="58179" checksum="B43D46BB256AC9E9" modified="2016-01-20" version="1">MSPSMAAVNAELQQQHGGSPPHFLVVAYGIQSHINPCRLLAHRLACLGIVSGSGPVLATLAVPLSAHRRMFPNHPSGNTADSDSDGVISYAPYSDGLDDGSPMPRDAEGKARVRRASFEGLSSVVASLAALGRPVTCVVVSMVHPAALDVARATALPLAVFWIQPATVLAAYYHFFHDDGGHYKELVTSHAADPDFEVSIPGLSLRRRPLRIRDFPTFLVDTTGSDIASSVNEALRELFEFMDQQGKNNAKVLVNTMEELEPSAVAAMAEHLDLFPVGPVVASGSSNNNASRNIHLFDHDNKAQYISWLDAQPASSVIYVSFGSIWTYSKPQMEEIAAGLKQCNRPFLLVVRKDGRQDQDVSSCLDELCAQELGIVVAWCDQAAVLAHPAVGCFVTHCGWNSTLEAAAHGVPVVAAPGMFDQPTNAFLAEQEWGAGVRVEKEKEDEGGVFAGAELARCVQVVMGDGGRGMEIRGRAQALKEIARKAAADGGPAEKSLRNFVMAVVGQGSSSDNSNTSKLVEADVVEKLKGLEVSTGSSNGNVTSEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QPG0</accession>
    <name>A0A0Q3QPG0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase C45 hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100830403</name>
        <name evidence="2" type="ORF">BRADI_2g07272v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565709.1">
        <property type="nucleotide sequence ID" value="XM_003565661.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3QPG0"/>
    <dbReference type="STRING" id="15368.A0A0Q3QPG0"/>
    <dbReference type="EnsemblPlants" id="KQK03346">
        <property type="protein sequence ID" value="KQK03346"/>
        <property type="gene ID" value="BRADI_2g07272v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830403"/>
    <dbReference type="Gramene" id="KQK03346">
        <property type="protein sequence ID" value="KQK03346"/>
        <property type="gene ID" value="BRADI_2g07272v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830403"/>
    <dbReference type="OrthoDB" id="189997at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3QPG0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.60.10">
        <property type="entry name" value="Penicillin V Acylase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047794">
        <property type="entry name" value="C45_proenzyme-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047801">
        <property type="entry name" value="Peptidase_C45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005079">
        <property type="entry name" value="Peptidase_C45_hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040521">
        <property type="entry name" value="C45_proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34180:SF1">
        <property type="entry name" value="BETA-ALANYL-DOPAMINE_CARCININE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34180">
        <property type="entry name" value="PEPTIDASE C45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03417">
        <property type="entry name" value="AAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C45 hydrolase" evidence="1">
        <location>
            <begin position="132"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="364" mass="39746" checksum="819810CE57B2FF9D" modified="2016-01-20" version="1">MESAGEMEVFDAGRCNGGYDLGFAVGQRFSEMIRSRMRGDMFLQEQLLPFASTAPAQPLLAALQAANRERYPRYWDELVGIADGSGAPLLQVILVNFRKEIRPFIPKKGQDHGRKDEEADDDCSDVLLVSESAAFAAHNEDASPALLGHTYVVKATSPDGASSFTAYTYAGELPTCAFGFNTNRVAFTLDSVPPAIGEVAAGAIALNFVSRDLLEATDLDDAMRRVCSPGVSVGHCYNLMDVRARRIVTVETASRNRFAVHEAGPAPSFHANMYRYLQVEQVQDENSMARERRAAQCAMGSKEEALSVLGDATDEKYPIFMTGPTLYTLCTVLVDLDQETMTICKGNPKNGDSIRVLRMTQTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R6K8</accession>
    <name>A0A0Q3R6K8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g45656v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R6K8"/>
    <dbReference type="EnsemblPlants" id="KQK09034">
        <property type="protein sequence ID" value="KQK09034"/>
        <property type="gene ID" value="BRADI_2g45656v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09034">
        <property type="protein sequence ID" value="KQK09034"/>
        <property type="gene ID" value="BRADI_2g45656v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3R6K8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3R6K8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036297626" description="Secreted protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK09034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="15623" checksum="03D79252141C9132" modified="2016-01-20" version="1">MTWALLLTGVAFALRCGGESFSREEHGPVCELLRLHPGVPPAVHAAASRVAVKVAAYIQGWHNAIVRALCRRLHLLDVCPPRVALQFNRAQGHQARASGDKEDVEAGRRTSHGSHGLPMCNERELCVVCRRSCYVGFGNIGSMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3R8C0</accession>
    <name>A0A0Q3R8C0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ras-related protein Rab7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100821204</name>
        <name evidence="10" type="ORF">BRADI_2g48120v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Lipid-anchor</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569676.1">
        <property type="nucleotide sequence ID" value="XM_003569628.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3R8C0"/>
    <dbReference type="FunCoup" id="A0A0Q3R8C0">
        <property type="interactions" value="1268"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3R8C0"/>
    <dbReference type="EnsemblPlants" id="KQK09458">
        <property type="protein sequence ID" value="KQK09458"/>
        <property type="gene ID" value="BRADI_2g48120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821204"/>
    <dbReference type="Gramene" id="KQK09458">
        <property type="protein sequence ID" value="KQK09458"/>
        <property type="gene ID" value="BRADI_2g48120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821204"/>
    <dbReference type="OrthoDB" id="1436450at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3R8C0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01862">
        <property type="entry name" value="Rab7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000295">
        <property type="entry name" value="Ras-related protein Rab7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF2">
        <property type="entry name" value="RAS-RELATED PROTEIN RABG3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK09458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23167" checksum="5E861DDADDF8AC08" modified="2016-01-20" version="1">MSTSRRRTLLKVIVLGDSGVGKTSLMNQYVHNKFSQQYKATIGADFVTKEVLIEDRLVTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNVNRSFDTLNTWHDEFLNQASPSDPKTFPFILLGNKIDVDGGKSRVVSEKKAIEWCSSKGNIPYYETSAKEDYNVDDAFLSVAKLALEHERDQDIYFQTVADPVPETEQRGGCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CFM8</accession>
    <name>A0A2K2CFM8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100829562</name>
        <name evidence="2" type="ORF">BRADI_5g06207v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CFM8"/>
    <dbReference type="FunCoup" id="A0A2K2CFM8">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT60831">
        <property type="protein sequence ID" value="PNT60831"/>
        <property type="gene ID" value="BRADI_5g06207v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60831">
        <property type="protein sequence ID" value="PNT60831"/>
        <property type="gene ID" value="BRADI_5g06207v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CFM8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08267">
        <property type="entry name" value="MDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052733">
        <property type="entry name" value="Chloroplast_QOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44013:SF6">
        <property type="entry name" value="OS04G0359100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44013">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C16A3.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="46"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT60831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="356" mass="37453" checksum="B616CECEE2F480B6" modified="2018-03-28" version="1">MICSRAFFSSSIQESTQATLAQEVKIMGDPARPETMRAVQYSGYGGGAAALKYVEIPVPSLKKDEVLIKVEAASINPADCNIQKGLLRPFVPRFPFIPVSDVAGEIVEVGAAVSEFKVGDRVVSKLIFWKAGGLAEYVAASESVTVALPAGVSTVDAAGLPVAGLTALQAVRAIGTKFDGTGSGSNILITAGSGGIGTYAVQLAKLGNHNVTATCGARNLELVADMGADEVLDYKTPEGAALKNNSGKKYDYIVNTTNAGKWSAFKPALSSHGRVVDLAPNLANFVASMLTLFSKKKKLSTVLLSLGMEDLRFLLELVKEGKLKTVVDSRHPFDKAADAWEKSMSGHATGKVIVEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CFP3</accession>
    <name>A0A2K2CFP3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100822914</name>
        <name evidence="11" type="ORF">BRADI_5g06765v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579573.2">
        <property type="nucleotide sequence ID" value="XM_003579525.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CFP3"/>
    <dbReference type="STRING" id="15368.A0A2K2CFP3"/>
    <dbReference type="EnsemblPlants" id="PNT60848">
        <property type="protein sequence ID" value="PNT60848"/>
        <property type="gene ID" value="BRADI_5g06765v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822914"/>
    <dbReference type="Gramene" id="PNT60848">
        <property type="protein sequence ID" value="PNT60848"/>
        <property type="gene ID" value="BRADI_5g06765v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822914"/>
    <dbReference type="OrthoDB" id="10253408at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CFP3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000057">
        <property type="entry name" value="Cysteine protease RD19A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="10">
        <location>
            <begin position="75"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="9">
        <location>
            <begin position="164"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT60848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="399" mass="43747" checksum="B719302F3B04A950" modified="2018-03-28" version="1">MNRSRHPHISKPQRKKTISNHPIMARLRRLPIVVAAVLLLSGVAALSSPVEDPLIEQVVGGDEKNELELNAEAHFASFVQRFNKSYRDADEHAHRLSVFTANLRRARRHQRLDPSAVHGVTKFSDLTPDEFRDRFLGLRKYRRSFLKGLSGSAHDAPALPTDGLPTEFDWREHGAVGPVKDQGSCGSCWSFSTSGALEGAHYLATGKLEVLSEQQMVDCDHECDPSEPRACDAGCNGGLMTTAFSYLAKAGGLETEKDYPYTGRGGACKFDKSKIAAQVKNFSTVAVDEDQIAANLVKHGPLAIGINAVFMQTYIGGVSCPFICGRHLDHGVLLVGYGSAGYAPLRFKEKPYWIIKNSWGENWGESGYYKICRGAHVKNKCGVDSMVSTVTAIHTSNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CHC5</accession>
    <name>A0A2K2CHC5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g15045v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CHC5"/>
    <dbReference type="EnsemblPlants" id="PNT61418">
        <property type="protein sequence ID" value="PNT61418"/>
        <property type="gene ID" value="BRADI_5g15045v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61419">
        <property type="protein sequence ID" value="PNT61419"/>
        <property type="gene ID" value="BRADI_5g15045v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61420">
        <property type="protein sequence ID" value="PNT61420"/>
        <property type="gene ID" value="BRADI_5g15045v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61418">
        <property type="protein sequence ID" value="PNT61418"/>
        <property type="gene ID" value="BRADI_5g15045v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61419">
        <property type="protein sequence ID" value="PNT61419"/>
        <property type="gene ID" value="BRADI_5g15045v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61420">
        <property type="protein sequence ID" value="PNT61420"/>
        <property type="gene ID" value="BRADI_5g15045v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CHC5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT61420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="91" mass="9837" checksum="5C3B7D22A50B7FBF" modified="2018-03-28" version="1">MAVLIVINVHPKITIQLESLIWTMETGQSAVSSALSWVRAFHTSTSPSLPRDDDAQRLGIIMDPLALVCNLTCTKFLVVASFVLSPGIPAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CKM2</accession>
    <name>A0A2K2CKM2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cell wall hydroxyproline-rich glycoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_4g05479v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CKM2"/>
    <dbReference type="STRING" id="15368.A0A2K2CKM2"/>
    <dbReference type="EnsemblPlants" id="PNT62583">
        <property type="protein sequence ID" value="PNT62583"/>
        <property type="gene ID" value="BRADI_4g05479v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62583">
        <property type="protein sequence ID" value="PNT62583"/>
        <property type="gene ID" value="BRADI_4g05479v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CKM2"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CKM2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000224">
        <property type="entry name" value="Leucine-rich repeat extensin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051582">
        <property type="entry name" value="LRR_extensin-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32093">
        <property type="entry name" value="LEUCINE-RICH REPEAT EXTENSIN-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32093:SF124">
        <property type="entry name" value="POLLEN-SPECIFIC LEUCINE-RICH REPEAT EXTENSIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036043221" description="Cell wall hydroxyproline-rich glycoprotein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="431"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="440"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="485"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041871"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT62583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="548" mass="57931" checksum="6275486112AA5780" modified="2018-03-28" version="1">MASHGILVPLLFFFFLLSALSDATLKEGSNGELPDDFEFDIHVDVTFANERLRRAYIALQAWRKAIYSDPKNFTGGWAGADVCAYFGVSCVQALDDPNATVVAAVDLNGGDIAGHLPPELGLLADIAVFHINSNRFCGIIPETFSRLSLLHELDVSNNRFVGPFPKVVLHIPVLKYLDLRFNDFEGELPKELFEKSLDAVVLNSNRFVGFIPDNIGNSTASMVVLANNKFVGCIPRGVGMMAGTLDELVLLNNRLDGCMPPELGMLGNTTVVDVSGNALAGTLPEELQGLKKVEQLDVSRNLMAGAVGEKVCTLPALANFSFGGNFFSQEAPACVPKEGGDVEMDDKGNCLPGRPAQKTPLECGPVLARPVDCSTNVCSARPSKPKPVYPPVVGPYMPKAPAPPKYPPVVGPYMPKAPPPVPVMSPPLPVKSPPPPVPLSTSTSEIPATTSARKLTASTCKIPSTTSTGKLATSACKIRTTPSAVPSPPPPAPVRSPPPPVFPPPPPAVPSPPPPAPSPPPPAPAGIILPPIKAQNYMSPPPPQFQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CL22</accession>
    <name>A0A2K2CL22_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g07595v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT62725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CL22"/>
    <dbReference type="EnsemblPlants" id="PNT62725">
        <property type="protein sequence ID" value="PNT62725"/>
        <property type="gene ID" value="BRADI_4g07595v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62725">
        <property type="protein sequence ID" value="PNT62725"/>
        <property type="gene ID" value="BRADI_4g07595v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CL22"/>
    <dbReference type="OrthoDB" id="676984at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT62725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="18331" checksum="F8C46D0B25046B56" modified="2018-03-28" version="1">MEWGSFELQVLLSGLLPNPKLETVVLSICTRVSNGLGAGRPQAARLAARVVMLLALAVGASQGLAMFLLRNVWGYAYSNDEQVAGYIARMMPILAISIVFDSLQCVLSGVVRGCGQQKTGAFVNLVAYYIVGVPAAFFFAFICHLGGMALKAKDRVFTSSLRADMTNDMTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CQC9</accession>
    <name>A0A2K2CQC9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_4g26548v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CQC9"/>
    <dbReference type="EnsemblPlants" id="PNT64245">
        <property type="protein sequence ID" value="PNT64245"/>
        <property type="gene ID" value="BRADI_4g26548v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64245">
        <property type="protein sequence ID" value="PNT64245"/>
        <property type="gene ID" value="BRADI_4g26548v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CQC9"/>
    <dbReference type="OrthoDB" id="21204at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF129">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <position position="132"/>
        </location>
    </feature>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT64245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="132" mass="14109" checksum="5D2D2741939F61FA" modified="2018-03-28" version="1" fragment="single">MALVGRPRTMAVRVPDYVLHDDDGSSSSRSSGAFFARRRGPPTYFSSSFSGPGPASSDAMAALHVLPEPETTREDCCAVCLDGLEDTRGNGEPAAIRSMPCSHSFHEGCIFRWLAVSRVCPCCRFPLPSAAA</sequence>
</entry>
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    <accession>A0A2K2CS41</accession>
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        <name type="common">Purple false brome</name>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_4g33899v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64846">
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        <property type="gene ID" value="BRADI_4g33899v3"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT64846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15514" checksum="2B80A3279199C091" modified="2018-03-28" version="1" fragment="single">FWSTGGPNAINDMLWHIVDVRDVADALLLVYEKPESSGRYICAPNYMRAKAVLELLKKTYPGYNYVKCKAGADHDTRITPMSSEKLRNLGWKPREVEETLLDSVEYCRETGILQDVEGRSYRLPDVFRLFHAAEE</sequence>
</entry>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1">
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        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblPlants" id="PNT67284">
        <property type="protein sequence ID" value="PNT67284"/>
        <property type="gene ID" value="BRADI_3g23343v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g23343v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CZ25"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT67284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="75" mass="8424" checksum="75A8C453C1D2FE6A" modified="2018-03-28" version="1">MVATSTTNVTQPPVGDGLAALLWRTSTRRWLSLQTPCHDSPMLRPLSMRRWPAFAHVPLLKKVPEATLLLNLLFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D0S7</accession>
    <name>A0A2K2D0S7_BRADI</name>
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            <fullName evidence="1">VAN3-binding protein-like auxin canalisation domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g33306v3</name>
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    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="STRING" id="15368.A0A2K2D0S7"/>
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        <property type="gene ID" value="BRADI_3g33306v3"/>
    </dbReference>
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        <property type="entry name" value="VAB"/>
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        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31351:SF39">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="17"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="113" mass="12301" checksum="F1413F91829708E5" modified="2018-03-28" version="1">MEGLDSLTSLFLPPCSVRSAVGASSPGDLTPLTAAAATALRGAAAMKQRVQREARSSASVLPYEKGYSWSPDVWCKEGELLKRTRKGTDYRSGVLKYSEHSVMVMDHKSKSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D6G8</accession>
    <name>A0A2K2D6G8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="primary">LOC104582997</name>
        <name evidence="1" type="ORF">BRADI_2g01692v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_2g01692v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT69863"/>
        <property type="gene ID" value="BRADI_2g01692v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
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        <property type="expression patterns" value="baseline"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="PNT69863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="139" mass="14378" checksum="B7AEACA5AF4E8F80" modified="2018-03-28" version="1">MPVHKVRHARVGMLRLPAMGPPRHLMLVEGNDDSGFLLTVGRGQAGITAVSAVYIRAGGPPWYAVKMWANGPPPAPGMKTDSRAHAVLVEIVAVCGAAPGAVDVKALPVLTVPRNFLVGGAAGAADMVLPVSVRVDKMS</sequence>
</entry>
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    <accession>A0A2K2D9T7</accession>
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            <fullName evidence="2 3">Uncharacterized protein</fullName>
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        <name type="synonym">Trachynia distachya</name>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT71050"/>
        <property type="gene ID" value="BRADI_2g22215v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_2g22215v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT71050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="68" mass="7950" checksum="DEC21E1ACC81FE80" modified="2018-03-28" version="1">MDILFYVRSCYWDDAEDDASSFAMSREDNTSHLVCMATQTQRRLIVIIVNYSCDVYTAYMILYSIPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DBL3</accession>
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        <recommendedName>
            <fullName evidence="2">Obg domain-containing protein</fullName>
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    <gene>
        <name evidence="3" type="ORF">BRADI_2g33263v3</name>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodium</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT71652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A2K2DBL3"/>
    <dbReference type="EnsemblPlants" id="PNT71652">
        <property type="protein sequence ID" value="PNT71652"/>
        <property type="gene ID" value="BRADI_2g33263v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71652">
        <property type="protein sequence ID" value="PNT71652"/>
        <property type="gene ID" value="BRADI_2g33263v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DBL3"/>
    <dbReference type="OrthoDB" id="347018at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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        <location>
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            <end position="108"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
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        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT71652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="108" mass="10802" checksum="536659E6A58F0177" modified="2018-03-28" version="1">MPPASPNLLKKCREGLPSSDAGAPAEGSGCARGGDGGNGCVSQSSQGRTAKAGLTDFSSLQHHTKAGRGGHGLPKNQIGTRGSDKVAQVPVGTVIHLGSTGLHRHLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DC27</accession>
    <name>A0A2K2DC27_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">BRADI_2g36049v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Bd21</strain>
            <strain evidence="2">cv. Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_014898951.2"/>
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    <dbReference type="STRING" id="15368.A0A2K2DC27"/>
    <dbReference type="EnsemblPlants" id="PNT71828">
        <property type="protein sequence ID" value="PNT71828"/>
        <property type="gene ID" value="BRADI_2g36049v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583232"/>
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        <property type="protein sequence ID" value="PNT71828"/>
        <property type="gene ID" value="BRADI_2g36049v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583232"/>
    <dbReference type="OrthoDB" id="785312at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Diaminopimelate Epimerase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR003719">
        <property type="entry name" value="Phenazine_PhzF-like"/>
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    <dbReference type="PANTHER" id="PTHR13774:SF43">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774">
        <property type="entry name" value="PHENAZINE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02567">
        <property type="entry name" value="PhzC-PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54506">
        <property type="entry name" value="Diaminopimelate epimerase-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT71828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="146" mass="15546" checksum="53FAC794FF424F3A" modified="2018-03-28" version="1">MQVNAFADEAFKGNPAAVCLLEGEDVNGADERWLQSVATEFNAPMTAFLSRTTHAAAAAAPPRFHIRWFSPVTEVTLCGHATLAAAHFLFTAVLIAAEDGDDMMAEFVTKSGMILAAKKVPAPVLQSQVSSSTCPAFIELDFPICT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DDA2</accession>
    <name>A0A2K2DDA2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100822230</name>
        <name evidence="12" type="ORF">BRADI_2g41900v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569258.2">
        <property type="nucleotide sequence ID" value="XM_003569210.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DDA2"/>
    <dbReference type="FunCoup" id="A0A2K2DDA2">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72256">
        <property type="protein sequence ID" value="PNT72256"/>
        <property type="gene ID" value="BRADI_2g41900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822230"/>
    <dbReference type="Gramene" id="PNT72256">
        <property type="protein sequence ID" value="PNT72256"/>
        <property type="gene ID" value="BRADI_2g41900v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822230"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DDA2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01989">
        <property type="entry name" value="USP_STK_Ubox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000498">
        <property type="entry name" value="U-box domain-containing protein 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051348">
        <property type="entry name" value="U-box_ubiquitin_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647:SF118">
        <property type="entry name" value="OS01G0581400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647">
        <property type="entry name" value="OS02G0152300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="441"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="353"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT72256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="752" mass="83624" checksum="79F8774F09866CA2" modified="2018-03-28" version="1">MMMFGAPLSPEEPRLTSPPSLHQASSTIVVAIDRDRNSQLAMKWVVDHLLNSAAHIILLHVAAHHPAANHGFAMAEPTPGALEAEMKEIFVPFRGFCDKNGVEQSEVILEEADVSKAILEYITANKIQSIALGASNRNAFTKKWKNPDVPSSLMKGAPDYCNIYVVAKGKPVNVRLAKCGVSTDDSDFALGPTYSRRSSRNHLPPPMPESLSCRRSIDRNIPELTTRPPFRERSLPASVTKPLLLCGRVDSTDGTYRSTRRSASHDSVDVDPDFAQAVHSSSMDFSENLDALNLSPRESCSPLSGAQREVEGEMRRLRLELKQTMDMYNAACKEAISAKQRAREMHLLSMEEARLLEESRQAEEAALHQAEMEKLKCRAAMEAAEAAQRLADLEAQRRRNAEVRARRESDEKVRALDAISSHDFRCRKYTIDEIELATERFDEKLKIGEGGYGPVYSASLDHTPVAIKVLRPDAQQGRKQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVYEYMENGSLEDRLFRRGGTPPMPWSQRFRISAEIATALLFLHQTRPEPLVHRDLKPANILLDRNYVSKISDVGLARLVPPAVADSVTRYRLTATAGTFCYIDPEYQQTGKLGVKSDIYSLGVLLLQVITARPPMGLTHHVEKAIESDTFAQMLDITVKDWPVEDALGFAKLALKCTEMRRRDRPDLGTVILPELNRLRNLGIAYDQVRAAAAATANVSDSSAHAQEIISSHTVDGAWRTAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGR5</accession>
    <name>A0A2K2DGR5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g58945v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGR5"/>
    <dbReference type="EnsemblPlants" id="PNT73476">
        <property type="protein sequence ID" value="PNT73476"/>
        <property type="gene ID" value="BRADI_2g58945v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73476">
        <property type="protein sequence ID" value="PNT73476"/>
        <property type="gene ID" value="BRADI_2g58945v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DGR5"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT73476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="74" mass="8644" checksum="A52DA7BD7308992A" modified="2018-03-28" version="1">MAPYCVHATCVLESPAVSDWKIRWINTVEIDLHIQCFLSDPYYLCQICLNIDVSMFKKRLDTCNVSTNNMDQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGW9</accession>
    <name>A0A2K2DGW9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">thiamine diphosphokinase</fullName>
            <ecNumber evidence="4">2.7.6.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100840605</name>
        <name evidence="14" type="ORF">BRADI_2g59740v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP). TPP is an active cofactor for enzymes involved in glycolysis and energy production. Plant leaves require high levels of TPP for photosynthesis and carbohydrate metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>thiamine + ATP = thiamine diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11576"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18385"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the thiamine pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.2" evidence="4"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565008.2">
        <property type="nucleotide sequence ID" value="XM_003564960.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGW9"/>
    <dbReference type="FunCoup" id="A0A2K2DGW9">
        <property type="interactions" value="1165"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DGW9"/>
    <dbReference type="EnsemblPlants" id="PNT73522">
        <property type="protein sequence ID" value="PNT73522"/>
        <property type="gene ID" value="BRADI_2g59740v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840605"/>
    <dbReference type="Gramene" id="PNT73522">
        <property type="protein sequence ID" value="PNT73522"/>
        <property type="gene ID" value="BRADI_2g59740v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840605"/>
    <dbReference type="OrthoDB" id="25149at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DGW9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.320:FF:000001">
        <property type="entry name" value="Thiamine pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10240:FF:000001">
        <property type="entry name" value="Thiamine pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.320">
        <property type="entry name" value="Thiamin pyrophosphokinase, thiamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036371">
        <property type="entry name" value="TPK_B1-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01378">
        <property type="entry name" value="thi_PPkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF12">
        <property type="entry name" value="THIAMINE PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63862">
        <property type="entry name" value="Thiamin pyrophosphokinase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamin pyrophosphokinase thiamin-binding" evidence="13">
        <location>
            <begin position="283"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="PNT73522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="359" mass="39550" checksum="80D2EEE4DBC5FCD8" modified="2018-03-28" version="1">MPLPCRQQLRPGPNRQRLRQLGSTKKRKKGYAPSTRPLVKGSNLCFLFVKHKYTNSFRVPCSLLIISYSGSAPHRPRRPRHATSPPPGGDQSAIAPMPLPTMTHSSSFLLPSPPAEAAAAADAVAYAVIVLNQRLPRFASLLWTRAQLRVCADGGANRLFDGMPELHPGEDPDEVRARYKPDAIKGDMDSIRPEVKEFYSNLGAKIVDESHDQDTTDLHKCISFITRNPPGPDKANLCILVLGALGGRFDHEMGNINVLYRFSNIKIILLSDESLIFLLPKTHNHEILVDQSIEGPHCGLIPMGMPSSSSTTTGLRWNLDNTSMSYGGLVSTSNIVDDKKVMVTSDSDLIWTISLRNHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DH84</accession>
    <name>A0A2K2DH84_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ceramidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_2g61534v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkaline ceramidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DH84"/>
    <dbReference type="FunCoup" id="A0A2K2DH84">
        <property type="interactions" value="604"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DH84"/>
    <dbReference type="EnsemblPlants" id="PNT73647">
        <property type="protein sequence ID" value="PNT73647"/>
        <property type="gene ID" value="BRADI_2g61534v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73647">
        <property type="protein sequence ID" value="PNT73647"/>
        <property type="gene ID" value="BRADI_2g61534v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DH84"/>
    <dbReference type="OrthoDB" id="5562961at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DH84">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008901">
        <property type="entry name" value="ACER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34368">
        <property type="entry name" value="OS01G0962200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34368:SF1">
        <property type="entry name" value="OS01G0962200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05875">
        <property type="entry name" value="Ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT73647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="33935" checksum="7023FC2B752542CB" modified="2018-03-28" version="1">MDDDAGRRKKKWVAWAAAAAIFVVLMLVTPAIPQDEDYHDFADQRDLFLGIPNTLNVVSNIPFFFVGVVGLILCHYKNYFRLCSQGELWSWTLFFAGVTAVGFGSSYYHLNPNNATLVWDRLPMTIAFTSIMAIFIIERVDDRAGTKSLAPLVFAGALSILYWRFFDDLRPYAVIQFVPCIAIPVMAIVIPPMYTHSSYWLWAAGFYLLAKVEEAADKPIYRWTHQIVSGHTLKHLCAAMVPVFLTLMLAKRTIEPERVSLLQMWKISWVSMRARRSRDSNTVDVNYDYAAVSTTPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DKH8</accession>
    <name>A0A2K2DKH8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g21632v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
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            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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    </evidence>
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</entry>
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    </evidence>
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    <sequence length="149" mass="15809" checksum="D27306998E640376" modified="2018-03-28" version="1">MRPLLGCLLLGAGAAVSSGGRVQGEETRRRSGGSLGRGGRPPPRAGEPPRPRQAAMRVAAENWGRGRGRAPSRDTGKGPSSLTARSSSQQVDSQGENREKNAMGGSKQRNAGTSTNHLGQSCREIKGSFDSKDFHRDFGGFESLEFFPI</sequence>
</entry>
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            <strain evidence="3 4">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
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            <strain evidence="8 9">Bd21</strain>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558804.2">
        <property type="nucleotide sequence ID" value="XM_003558756.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DV80"/>
    <dbReference type="FunCoup" id="A0A2K2DV80">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DV80"/>
    <dbReference type="EnsemblPlants" id="PNT78189">
        <property type="protein sequence ID" value="PNT78189"/>
        <property type="gene ID" value="BRADI_1g75087v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825246"/>
    <dbReference type="Gramene" id="PNT78189">
        <property type="protein sequence ID" value="PNT78189"/>
        <property type="gene ID" value="BRADI_1g75087v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825246"/>
    <dbReference type="OrthoDB" id="1728340at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DV80">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="94"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="261"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT78189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="447" mass="49044" checksum="CE09B51B65684ABB" modified="2018-03-28" version="1">MPLPVACCCSGRVESTAVIPMHASGAAAASGLQEPLVAHGLYKHRPLVPSSDAAFLLEAKKLLTHSVLFATAERAYIFEIMGGSMWMRYAPHNMMILVQLSYTLMYFVTEDAFNRGLNPYVYVTYRHLLVALLLCPFAYFHEKKLRPKMTLMLFLEIFVLSLLGVSLTLNMYFASLKYTSPTFVTAMANTVASITFAIAIVLRMEIVDVKSPRGLAKVAGTAVSFAGVTTMTFYKGAAIASPWKAPIHIHGSNAVHGSWVKGSLLAVASCVCWSIWYIMQAGSVERYPAELSLTAWMATVGGIQSAAFTVLLQHRKEDWLVGFGLNFWCIIYSGIACSGFAVFAQLWCTEKKGPVFVTMFNPVSTIMVAILAYFICGENLYVGSIIGGGVVILGMYMLLWGKDKDQEYNASSSKELQGSDLDWEKQAKMADVSSVQNDSQPETKTMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DVD3</accession>
    <name>A0A2K2DVD3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_1g75681v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DVD3"/>
    <dbReference type="STRING" id="15368.A0A2K2DVD3"/>
    <dbReference type="EnsemblPlants" id="PNT78234">
        <property type="protein sequence ID" value="PNT78234"/>
        <property type="gene ID" value="BRADI_1g75681v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78234">
        <property type="protein sequence ID" value="PNT78234"/>
        <property type="gene ID" value="BRADI_1g75681v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DVD3"/>
    <dbReference type="OrthoDB" id="687450at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DVD3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000028">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF34">
        <property type="entry name" value="CHAPERONE PROTEIN DNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036319280" evidence="4">
        <location>
            <begin position="28"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT78234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="274" mass="29766" checksum="03ADE79E2F70F705" modified="2018-03-28" version="1">MATRLLYQLGVLLLLAIAAAWTSGAAAFGLPRVSSGYCVWTSPFNTVNATAIHIGNTKSCISGYGGRLDPYTMYQFCIPSWVAFTANSTLFGEAAMNHAGVSPGTAVSGFKRLLGVRVFAGILMGELKKTAEAWLGREIMYAVVTVPADFNDAQRSHVTAAAELRGGFHAAKAVDEEVAAAAAYRLHEKRGDGKAILVFHLGGRTCHATKFRFHNGTARLLVERHDAYLGGDDFTCRIVDYFVKLIKEKHHRDISEDEGALRKLRADSEVLKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1GMU7</accession>
    <name>I1GMU7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100834958</name>
        <name evidence="10" type="ORF">BRADI_1g07260v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558752.1">
        <property type="nucleotide sequence ID" value="XM_003558704.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GMU7"/>
    <dbReference type="FunCoup" id="I1GMU7">
        <property type="interactions" value="214"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GMU7"/>
    <dbReference type="EnsemblPlants" id="KQK12989">
        <property type="protein sequence ID" value="KQK12989"/>
        <property type="gene ID" value="BRADI_1g07260v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834958"/>
    <dbReference type="Gramene" id="KQK12989">
        <property type="protein sequence ID" value="KQK12989"/>
        <property type="gene ID" value="BRADI_1g07260v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834958"/>
    <dbReference type="eggNOG" id="KOG0048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028567_6_2_1"/>
    <dbReference type="OMA" id="FGMSTDQ"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor MYB36"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transcription factor RAX3"/>
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        <property type="entry name" value="Homeodomain-like"/>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Myb_dom"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48000:SF67">
        <property type="entry name" value="MYB-LIKE DNA-BINDING DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48000">
        <property type="entry name" value="OS09G0431300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HTH_MYB"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="9"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="9"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="63"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="63"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK12989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="308" mass="34126" checksum="E6DE79DB0C570B12" modified="2012-06-13" version="1">MGRAPCCDKASVKKGPWSPEEDATLKSYIEQNGTGGNWIALPQKIGLKRCGKSCRLRWLNYLRPNIRHGGFSEEEDRIILSLYISIGSRWSIIAAQLPGRTDNDIKNYWNTRLKKKLFGKQSRKDQRQQHFARQGANGGQKEEVSRDAAGTNGLAAGAYNWHQHAMAMAMPVRPMSGSMVEGNRIGEEVDESIRKLLFKLGGSPFMSLPPPPVYGETPSFVPPSVHTTPPSEGGIQCSSILPALELDESFHFNQIKLDGLDCFFGMGDQNMRWNEVSPLVCPNNTVASSSQGIQQYCLVEEPVDLGMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1GRH0</accession>
    <name>I1GRH0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100823656</name>
        <name evidence="6" type="ORF">BRADI_1g18760v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK14815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562514.1">
        <property type="nucleotide sequence ID" value="XM_003562466.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GRH0"/>
    <dbReference type="STRING" id="15368.I1GRH0"/>
    <dbReference type="EnsemblPlants" id="KQK14815">
        <property type="protein sequence ID" value="KQK14815"/>
        <property type="gene ID" value="BRADI_1g18760v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74607">
        <property type="protein sequence ID" value="PNT74607"/>
        <property type="gene ID" value="BRADI_1g18760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823656"/>
    <dbReference type="Gramene" id="KQK14815">
        <property type="protein sequence ID" value="KQK14815"/>
        <property type="gene ID" value="BRADI_1g18760v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74607">
        <property type="protein sequence ID" value="PNT74607"/>
        <property type="gene ID" value="BRADI_1g18760v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823656"/>
    <dbReference type="eggNOG" id="KOG1039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059830_3_0_1"/>
    <dbReference type="OMA" id="HKLWSPW"/>
    <dbReference type="OrthoDB" id="1630758at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GRH0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000660">
        <property type="entry name" value="RING/U-box superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15315:SF88">
        <property type="entry name" value="OS07G0661600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15315">
        <property type="entry name" value="RING FINGER PROTEIN 41, 151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="146"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT74607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK14815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="242" mass="28114" checksum="68CAA23BB7B23872" modified="2012-06-13" version="1">MGRSFRDSLKLLEADIQHANSLASEFRREYDGACLQMRMSYCPAAHLFLFLVQWTDCNLAGALGLLRILIYKVYADGTTTMSTHERKASIREFYAVIYPSLGQLQEGINEVEDKKQKAICIERYRRPDEDHKRVISEIDDNIEEECGICMEINGKVVLPTCSHAMCIKCYRDWRSRSQSCPFCRDSLKRVNSADLWIYTDNSDIVDKATVRRENLRRLFMYIDKLPTVIPESVFEVYDSHVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWB7</accession>
    <accession>A0A0Q3JHG7</accession>
    <name>I1GWB7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100823443</name>
        <name evidence="12" type="ORF">BRADI_1g33117v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17227.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753385.1">
        <property type="nucleotide sequence ID" value="XM_014897899.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWB7"/>
    <dbReference type="STRING" id="15368.I1GWB7"/>
    <dbReference type="EnsemblPlants" id="KQK17227">
        <property type="protein sequence ID" value="KQK17227"/>
        <property type="gene ID" value="BRADI_1g33117v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823443"/>
    <dbReference type="Gramene" id="KQK17227">
        <property type="protein sequence ID" value="KQK17227"/>
        <property type="gene ID" value="BRADI_1g33117v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050764_1_0_1"/>
    <dbReference type="OMA" id="RASETHE"/>
    <dbReference type="OrthoDB" id="185618at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GWB7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13169">
        <property type="entry name" value="RanBD_NUP50_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000353">
        <property type="entry name" value="Nuclear pore complex protein NUP50A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015007">
        <property type="entry name" value="NUP2/50/61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045207">
        <property type="entry name" value="RanBD_NUP50_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF142">
        <property type="entry name" value="RAN-BINDING PROTEIN 3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08911">
        <property type="entry name" value="NUP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RanBD1" evidence="11">
        <location>
            <begin position="323"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="196"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="464"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK17227.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="473" mass="50217" checksum="DBB4B0AE69A92006" modified="2012-06-13" version="1">MADEEHAQTSRKRVADKQINKDNPEPDDDSTEQEDGTFKKATEEVMATRRIVKVRRQQPKPAPSNPFSAIRFTPSDSSVQSSVPVSEPPPSDVTVTNVKDSCLSEKTNEGSNGSGKDALSATDKNADSNEVDEIQKDEAAPEESDAEDKSSAPTEAPSPLVETDDKADDAGDGTGEDKVVVGEPKEDNCKTSGMEGKTEDVEAEEKKAATEAGDEDKFSKDDAEKKDEAESRAKDGSCEQKDAEKSSPTPLFSFKNLSSGQNAFTGLAGTGFSGSSFSFGSVSKESSNAPLFGLKSDGSSFPSFNIGGTSNGSSASALATAAEAPKKFAMPEGPVETGEENEKAVFTAESAIYEYLDGGWKERGKGELKLNIPVSGSGERSRLIMRARGNYRLILNASLYEDMSLKDMDKKGVTFACINSIGQSPSGLTTFAVKFKDTGIREDFKAAVGAHKAKKTPDAALKTPENSPKASDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6M5</accession>
    <name>I1H6M5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100834956</name>
        <name evidence="4" type="ORF">BRADI_1g65700v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558168.1">
        <property type="nucleotide sequence ID" value="XM_003558120.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6M5"/>
    <dbReference type="FunCoup" id="I1H6M5">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK22188">
        <property type="protein sequence ID" value="KQK22188"/>
        <property type="gene ID" value="BRADI_1g65700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834956"/>
    <dbReference type="Gramene" id="KQK22188">
        <property type="protein sequence ID" value="KQK22188"/>
        <property type="gene ID" value="BRADI_1g65700v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834956"/>
    <dbReference type="eggNOG" id="ENOG502QS8H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040722_0_0_1"/>
    <dbReference type="OMA" id="IYFCDEC"/>
    <dbReference type="OrthoDB" id="294702at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H6M5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763:SF8">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="215"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK22188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="537" mass="59409" checksum="6FA3A66F0B2943D6" modified="2012-06-13" version="1">MAAAKTMTWHEELATLVGDTGVRLPGVGVDAAPAPAGVNRAAVGGRWYGEEEEEGKVEEGWAQQARGFAESTAEMLRELGLGVWDVAAQSLAGAEDSELARRLRRPAAAAGKRLSFMNEYLPEDRDPVRCWLVVAAVAFVALLVLGAGTGDETPVEQLRKLYISPPNAKRFQLPDGRHLAYEEKGVSAERARFSLIAPHSFLSSRLAGIPGINTSLLEEFGARLVTYDLPGFGESDPHPGRNLNSSALDMLHLADALGVADKFWVVGYSGGGMHAWSALRYIPDRVAGAAMFAPMANPYDSKMTKDEKRKTWDRWSTKRKLMHILARRFPSLLRLFYLRSFLSGKQGQPESWLSLSLGKKDKALLEGAMFNAFWEKDVAESVRQGDAKPFVEEAVMQVSDWGFSLSDIQMQKKEDQGLFELIKSLFSQAEREWVGFLGPIHIWQGMDDRVVPPSVTEYVRRMVPGATVHKLLDEGHFSYFCFCDECHRQIFSTLFGIPQGPISPVQESGEVAPEFAEETTASEEFAEQEQDTSECLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1H757</accession>
    <name>I1H757_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100846139</name>
        <name evidence="9" type="ORF">BRADI_1g67190v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT77694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558285.1">
        <property type="nucleotide sequence ID" value="XM_003558237.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H757"/>
    <dbReference type="STRING" id="15368.I1H757"/>
    <dbReference type="EnsemblPlants" id="KQK22433">
        <property type="protein sequence ID" value="KQK22433"/>
        <property type="gene ID" value="BRADI_1g67190v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT77694">
        <property type="protein sequence ID" value="PNT77694"/>
        <property type="gene ID" value="BRADI_1g67190v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846139"/>
    <dbReference type="Gramene" id="KQK22433">
        <property type="protein sequence ID" value="KQK22433"/>
        <property type="gene ID" value="BRADI_1g67190v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT77694">
        <property type="protein sequence ID" value="PNT77694"/>
        <property type="gene ID" value="BRADI_1g67190v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846139"/>
    <dbReference type="eggNOG" id="KOG2934">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103892_1_0_1"/>
    <dbReference type="OMA" id="QRNCEAV"/>
    <dbReference type="OrthoDB" id="422700at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H757">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040053">
        <property type="entry name" value="JOSD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006155">
        <property type="entry name" value="Josephin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291">
        <property type="entry name" value="JOSEPHIN 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291:SF0">
        <property type="entry name" value="JOSEPHIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02099">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01246">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50957">
        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="8">
        <location>
            <begin position="25"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT77694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="199" mass="22259" checksum="24217030392C77E3" modified="2012-06-13" version="1">MEPGAKSEANQNEEGSGAVGSSGGSSKVYHERQRMQFCLLHALNNLMQEKESFTRAELDGISENLVLTDPNKDKWTPLSLIFKPHHNALTGNYDVNVLITALETRKTNVVWHDRRKGASSIDLDAGALVGLMINVPVKRLRGLWTGRHWVAIRSIDGIWFNLDSDFSSPKQFQGKEQLIEFLDSILSQGGELMIVLRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAR6</accession>
    <name>I1HAR6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SKP1 component dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100837314</name>
        <name evidence="11" type="ORF">BRADI_1g78110v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends on the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of a SCF (SKP1-cullin-F-box) protein ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559016.1">
        <property type="nucleotide sequence ID" value="XM_003558968.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAR6"/>
    <dbReference type="STRING" id="15368.I1HAR6"/>
    <dbReference type="EnsemblPlants" id="KQK24093">
        <property type="protein sequence ID" value="KQK24093"/>
        <property type="gene ID" value="BRADI_1g78110v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT78360">
        <property type="protein sequence ID" value="PNT78360"/>
        <property type="gene ID" value="BRADI_1g78110v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT78361">
        <property type="protein sequence ID" value="PNT78361"/>
        <property type="gene ID" value="BRADI_1g78110v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837314"/>
    <dbReference type="Gramene" id="KQK24093">
        <property type="protein sequence ID" value="KQK24093"/>
        <property type="gene ID" value="BRADI_1g78110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78360">
        <property type="protein sequence ID" value="PNT78360"/>
        <property type="gene ID" value="BRADI_1g78110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78361">
        <property type="protein sequence ID" value="PNT78361"/>
        <property type="gene ID" value="BRADI_1g78110v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837314"/>
    <dbReference type="eggNOG" id="KOG1724">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2342932at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HAR6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009867">
        <property type="term" value="P:jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000022">
        <property type="entry name" value="SKP1-like protein 21 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component dimerisation" evidence="10">
        <location>
            <begin position="117"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="215"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="170"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT78360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="336" mass="38041" checksum="8C83FAAB4D61937D" modified="2012-06-13" version="1">MSESELSVIKPEALKTYIWLQCFDGSIQQVEEEVAMFCPMICREIVKNGTGSSKNHAIALPERVNPASLSLILDYCRFHQVPGRSNKERKSFDEKFVRIDTEKLCELTSAADSLQLKPLVDLTSRALARIIEGKTPEEIRDIFHLPDDLTEEEKLEPLKNINDDPRIRLLNRLYAKKRKELQERQKLKDIQEQKEQKDERSLDEILCFINGDGGSGGGKAAKNKKKNKRRKDHAKNPQKADPEPGNMEGAACAVVGVGTISRAPCQSSDVQDDAEYPFEDGDLDDGLDPALQEELDREVEDFARRLNSVWPERRRIESQLNGGNGSLQRFSGLNHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1HAR8</accession>
    <name>I1HAR8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100832526</name>
        <name evidence="10" type="ORF">BRADI_1g78120v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the expansin family. Expansin B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK24094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229338.1">
        <property type="nucleotide sequence ID" value="XM_010231036.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HAR8"/>
    <dbReference type="FunCoup" id="I1HAR8">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK24094">
        <property type="protein sequence ID" value="KQK24094"/>
        <property type="gene ID" value="BRADI_1g78120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832526"/>
    <dbReference type="Gramene" id="KQK24094">
        <property type="protein sequence ID" value="KQK24094"/>
        <property type="gene ID" value="BRADI_1g78120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832526"/>
    <dbReference type="eggNOG" id="ENOG502QRTE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027462_1_1_1"/>
    <dbReference type="OMA" id="NESWGAI"/>
    <dbReference type="OrthoDB" id="644057at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22275">
        <property type="entry name" value="DPBB_EXPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005795">
        <property type="entry name" value="LolPI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF21">
        <property type="entry name" value="EXPANSIN-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00829">
        <property type="entry name" value="LOLP1ALLERGN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094459" description="Expansin-like EG45 domain-containing protein" evidence="7">
        <location>
            <begin position="26"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="63"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="183"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK24094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK24094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="28419" checksum="45BD971F8253030D" modified="2012-06-13" version="1">MASSSSSTLLLAASILATLASSAHGIPKVPPGPNITATYGDKWLDAKSTWYGKPTGAGPKDNGGACGYKDVDKEPFSGMTSCGNTPIFRDGRGCGSCFEIKCTKPDACSGDPVLVHITDDNEEPIAAYHFDLSGHAFGSMAKKGKEQDLRSAGEVEIQFRRVKCKYPEGTKVTFHVEKGSSPNYLAILVKYVGGDGDVVAVDVKEKGKDEWVALKESWGAVWRLDTAKPLKGPLTVRYTTDGGTKGESEDVIPEDWKPDTMYESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1HC53</accession>
    <name>I1HC53_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC104583026</name>
        <name evidence="26" type="ORF">BRADI_2g03567v3</name>
    </gene>
    <organism evidence="27">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">Bd21</strain>
            <strain evidence="27">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">Bd21</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014755203.1">
        <property type="nucleotide sequence ID" value="XM_014899717.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HC53"/>
    <dbReference type="EnsemblPlants" id="KQK02770">
        <property type="protein sequence ID" value="KQK02770"/>
        <property type="gene ID" value="BRADI_2g03567v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583026"/>
    <dbReference type="Gramene" id="KQK02770">
        <property type="protein sequence ID" value="KQK02770"/>
        <property type="gene ID" value="BRADI_2g03567v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583026"/>
    <dbReference type="eggNOG" id="ENOG502QSHG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_22_2_1"/>
    <dbReference type="OMA" id="HDFSWNS"/>
    <dbReference type="OrthoDB" id="1896041at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048508">
        <property type="term" value="P:embryonic meristem development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009942">
        <property type="term" value="P:longitudinal axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009945">
        <property type="term" value="P:radial axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000192">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000260">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000679">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase RPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF63">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 24" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094458" description="non-specific serine/threonine protein kinase" evidence="24">
        <location>
            <begin position="24"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="627"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="690"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="718"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KQK02770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="968" mass="103884" checksum="2E68749D4B9246B5" modified="2012-06-13" version="1">MAAPYYHLLLILLLLHFTPLASPSAVADRAALLALKGAVTADPASLLSSWSSSPSSHYCHWRGVTCHPSTATVAAIDLPSASLSGTLDFPIPPRLLRLDLSGNNFSGPVPAAFLASQTLRALNLTSNRLTGPLLFPSPSSPAPCRSLTHLRLAANFLVGQIPRALAQCRSLRVLDLSRNVLEGAVPPALGRLAALRVLDVSRNSLTDRIPPQLAGCRNLAVLLLTNPTAPPGEDQQPEFNAFVGALPPEVLAIPALQVLWAPRANLDGRLLPGYRNNGSCSLRAVNLAQNYIAGAVPQWFGECGDLAFLDLSSNSLEGSMPAQLRIGCMKYLNVSRNSLSGLLLSSTDSKCSSRLIDADDDVLQYYEALVGSALISGPFGPVMGDSTSVALHDFSNNGFAGALPSLHLRLDGNHSYGLFLNSNMFNGTFSGGFFGFCKTANGAAVNLSSNQLSGTLDMLPSCLALQSFEAGHNRFSGSISPSIGDLHLLRSLVLRENNLAGQIPGRLGDLAALEVLDLSKNSLEGIMPPQLFGASRLKVLMLDHNRLSGSIPPSSSELAQLAVFDVSFNNLSGDIPSLRHFADCSSFAGNPLLSPCLGPNALVPPLAATDSGKQTQRSGGHLTRSKVLMVIVAAAATALVSFILAAFFFFLCERRKRAKIANLRRKVVVTFTDAPPELNYDNLIRATSNFSIQNLIGTGGFGATYKAELAPGYLVAVKRLAMGRFQGLQQFDAEIATLGRIRHRNLVTLIGYHIGESDTFLIYNYLPGGNLESFIHEMGSRKVSWAEVYTIGMDVAQALAFLHGSCTPRIIHRDIKPSNILLDDELNAYLSDFGLARLIEVAQTHATTDVAGTFGYVAPEYATTCRVSDKSDVYSFGVVLLELMSGKRSLDPSFSQFGDGFTIVAWGRMLMQEERTSEFFSPGLWDASPKDRLTEMLKVAISCTSESLAVRPSMRQVAARLKQLRNAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1HGL4</accession>
    <name>I1HGL4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100841724</name>
        <name evidence="14" type="ORF">BRADI_2g17077v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a long chain fatty alcohol + a fatty acyl-CoA = a long-chain alcohol wax ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:38443"/>
            <dbReference type="ChEBI" id="CHEBI:17135"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77636"/>
            <dbReference type="ChEBI" id="CHEBI:235323"/>
            <dbReference type="EC" id="2.3.1.75"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the long-chain O-acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231047.1">
        <property type="nucleotide sequence ID" value="XM_010232745.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HGL4"/>
    <dbReference type="STRING" id="15368.I1HGL4"/>
    <dbReference type="EnsemblPlants" id="KQK04969">
        <property type="protein sequence ID" value="KQK04969"/>
        <property type="gene ID" value="BRADI_2g17077v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841724"/>
    <dbReference type="Gramene" id="KQK04969">
        <property type="protein sequence ID" value="KQK04969"/>
        <property type="gene ID" value="BRADI_2g17077v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841724"/>
    <dbReference type="eggNOG" id="ENOG502QTZ2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027831_0_0_1"/>
    <dbReference type="OMA" id="FNCYIIS"/>
    <dbReference type="OrthoDB" id="619536at2759"/>
    <dbReference type="UniPathway" id="UPA00282"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HGL4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047196">
        <property type="term" value="F:long-chain-alcohol O-fatty-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000021">
        <property type="entry name" value="O-acyltransferase WSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045034">
        <property type="entry name" value="O-acyltransferase_WSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009721">
        <property type="entry name" value="O-acyltransferase_WSD1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004255">
        <property type="entry name" value="O-acyltransferase_WSD1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650">
        <property type="entry name" value="O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650:SF1">
        <property type="entry name" value="WAX ESTER SYNTHASE_DIACYLGLYCEROL ACYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06974">
        <property type="entry name" value="WS_DGAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03007">
        <property type="entry name" value="WS_DGAT_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-acyltransferase WSD1-like N-terminal" evidence="12">
        <location>
            <begin position="91"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="O-acyltransferase WSD1 C-terminal" evidence="13">
        <location>
            <begin position="386"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK04969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="537" mass="59365" checksum="1E147B6028F8B938" modified="2012-06-13" version="1">MDPLESTADAASPPDPSPRKRAAAAALTVDTSATPGPRRRRRSPVVRERESKARQETETADMEQPVSPAGRLFREAHFNCYIVALLGLGAPLDVEAARAGLLDTLVRHPRFSSVQVLDEANKNAKPRWVRTTVNLDDHVIFPDLDPAATSADPDKAMEDYVSSLSTRPMDHSRPLWEFHVLDFPTSEARAAVAIRMHHSLGDGVSLISLLMACTRSAADPARLPALPAQPTPPPGPGRGLAAMLAAWAAWAWALLVLAWNTLVDVARFVATSWFLRDERTPFMGVPGVEFRRKRFLNCTLSLDDVKFVKNALKCTVNDVLIGVTSAALSRYYFRKTASTDDTSGDRTKPQKDIRLRAALLVNIRKTPGLHTLAEMMDNSKNNTVKWGNQIGYIVLPFHIVMHDDPLEYIRRGKRTADRKKRSLEAVFTYWSGNLVVKLFGIKAAAALCYGMFTNTTMSFSSLAGPAEKVEFYGHPIVYIATSVYGHPHALTVHFQSYSNIVKLVLAVDDEQFPDSHQLLDDFAESLRLVRQAAASVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HH45</accession>
    <name>I1HH45_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_2g18447v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HH45"/>
    <dbReference type="FunCoup" id="I1HH45">
        <property type="interactions" value="369"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK05162">
        <property type="protein sequence ID" value="KQK05162"/>
        <property type="gene ID" value="BRADI_2g18447v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK05162">
        <property type="protein sequence ID" value="KQK05162"/>
        <property type="gene ID" value="BRADI_2g18447v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1584">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027239_0_1_1"/>
    <dbReference type="InParanoid" id="I1HH45"/>
    <dbReference type="OMA" id="WHHALEY"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="47"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK05162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="346" mass="38357" checksum="6F53B98D8A6D553A" modified="2012-06-13" version="1">MAELVIPSLPVETRCPPFPLRQYNGFWIPEPILPGVTAARARFEPRPSDVFLASVPKSGTTWLKALALATLNRAKHPPCDPDHPLRHRNPHDCVEFLEAPVAWSKDGGGGVFAALPSPRVIATHLPCSLLPGGITEEEGSGGRIVYICRDPKDTLVSTWLYIKKMVAFAGARANDDDGKLLVPRPTSFTIEEAFELFCDGRCVCGPQWRHVGGYWEESKRRPEKVLFLRYEEMLEDPMGNVRKLAEFMGCAFSAEEEEAGVVRHIVELCSLDVLKDMEVNKSGTQGYVKNESFFRKGVAGDWSNHMTPAMAARLDKIVKDELHGSGFRFAVAVSRCPPAEKNHSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HHB3</accession>
    <name>I1HHB3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100842129</name>
        <name evidence="13" type="ORF">BRADI_2g19040v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568048.1">
        <property type="nucleotide sequence ID" value="XM_003568000.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HHB3"/>
    <dbReference type="FunCoup" id="I1HHB3">
        <property type="interactions" value="1997"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HHB3"/>
    <dbReference type="EnsemblPlants" id="KQK05259">
        <property type="protein sequence ID" value="KQK05259"/>
        <property type="gene ID" value="BRADI_2g19040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842129"/>
    <dbReference type="Gramene" id="KQK05259">
        <property type="protein sequence ID" value="KQK05259"/>
        <property type="gene ID" value="BRADI_2g19040v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842129"/>
    <dbReference type="eggNOG" id="KOG3106">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057784_0_0_1"/>
    <dbReference type="OMA" id="QEVLWAF"/>
    <dbReference type="OrthoDB" id="7694678at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094624" description="ER lumen protein-retaining receptor" evidence="12">
        <location>
            <begin position="26"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK05259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="25438" checksum="13D1AFF5DF9F098A" modified="2012-06-13" version="1">MNAFRFLGDMTHLFSVLVLLLKIYANKSCSGVSRKTQELYLAVFVARYLDLFTDYISLYNSVMKVVFISSSAAIVWYMRRHPQVRRTYDREQDTFRHVVLLAGAVVLALIFNERFTFREICWAFSIYLEAVAIIPQLVLLQRSRNVDNLTGQYVLFLGAYRAFYILNWIYRYFTEGHQSRWIPWIAGLVQTGLYADFFYYYFLSWKNNVKLELPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1HK56</accession>
    <name>I1HK56_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100829292</name>
        <name evidence="16" type="ORF">BRADI_2g27710v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Bd21</strain>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568603.1">
        <property type="nucleotide sequence ID" value="XM_003568555.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HK56"/>
    <dbReference type="STRING" id="15368.I1HK56"/>
    <dbReference type="EnsemblPlants" id="KQK06658">
        <property type="protein sequence ID" value="KQK06658"/>
        <property type="gene ID" value="BRADI_2g27710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100829292"/>
    <dbReference type="Gramene" id="KQK06658">
        <property type="protein sequence ID" value="KQK06658"/>
        <property type="gene ID" value="BRADI_2g27710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100829292"/>
    <dbReference type="eggNOG" id="KOG1744">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075666_1_0_1"/>
    <dbReference type="OMA" id="HYNKPST"/>
    <dbReference type="OrthoDB" id="1914959at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="56"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK06658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="153" mass="16574" checksum="9DC3E3A0B0C5FEA0" modified="2012-06-13" version="1">MAPKAEKKPAAKKPVEEEPAAEKAEKAPAGKKPKAEKRLPAGKTASKEGGGEKRGRKKGKKSVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HLD4</accession>
    <name>I1HLD4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100833990</name>
        <name evidence="6" type="ORF">BRADI_2g34350v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566498.1">
        <property type="nucleotide sequence ID" value="XM_003566450.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HLD4"/>
    <dbReference type="FunCoup" id="I1HLD4">
        <property type="interactions" value="606"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK07292">
        <property type="protein sequence ID" value="KQK07292"/>
        <property type="gene ID" value="BRADI_2g34350v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833990"/>
    <dbReference type="Gramene" id="KQK07292">
        <property type="protein sequence ID" value="KQK07292"/>
        <property type="gene ID" value="BRADI_2g34350v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833990"/>
    <dbReference type="eggNOG" id="ENOG502QS7F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035559_3_0_1"/>
    <dbReference type="OMA" id="KYCKKKP"/>
    <dbReference type="OrthoDB" id="191334at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HLD4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044174">
        <property type="entry name" value="BC10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042">
        <property type="entry name" value="CORE-2/I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE BC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK07292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="387" mass="44384" checksum="B95D89AE650AF94E" modified="2012-06-13" version="1">MRPRRHSYGRGGRSAAAVVLLVLCLCVTGVFLLLLHGSSPPLEAKEEEEAAAAGGERKQAVVVQAEVEEAPLPPGNSRVAFLFIARNRLPLDLVWDAFFRGDNEGRFSIYVHSRPGFVLTRATTRSRFFYNRQVNNSVQVDWGEASMIEAERILLSHALKDPFNERFVFVSDSCVPLYNFNYTHDYIMSASTSFVDSFADTKQGRYNPRMDPIIPVENWRKGSQWAVLIKKHAEVVVYDDVVLPEFRKHCRRRPLPEFWRDWDKPIPAEAWKAHNCIPDEHYVQTLLAQNGLEEELTRRSVTHSAWDLSSSKDRERRGWHPVTYKVSDATPALIKSIKDIDNIYYETEYRKEWCTSNEKPAPCFLFARKFTRGAGLKLLNSSFIAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1HRD8</accession>
    <name>I1HRD8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100844975</name>
        <name evidence="18" type="ORF">BRADI_2g49400v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003567102.3">
        <property type="nucleotide sequence ID" value="XM_003567054.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HRD8"/>
    <dbReference type="EnsemblPlants" id="KQK09656">
        <property type="protein sequence ID" value="KQK09656"/>
        <property type="gene ID" value="BRADI_2g49400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844975"/>
    <dbReference type="Gramene" id="KQK09656">
        <property type="protein sequence ID" value="KQK09656"/>
        <property type="gene ID" value="BRADI_2g49400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844975"/>
    <dbReference type="eggNOG" id="ENOG502QR6F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_99_2_1"/>
    <dbReference type="OMA" id="VSHKFHI"/>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HRD8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019199">
        <property type="term" value="F:transmembrane receptor protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000526">
        <property type="entry name" value="Kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000468">
        <property type="entry name" value="PTI1-like tyrosine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044812">
        <property type="entry name" value="CERK1/LYK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46204">
        <property type="entry name" value="CHITIN ELICITOR RECEPTOR KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46204:SF10">
        <property type="entry name" value="LYSM DOMAIN RECEPTOR-LIKE KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094799" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="25"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="17">
        <location>
            <begin position="165"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="364"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQK09656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="675" mass="72763" checksum="4FF7CFA438B49526" modified="2012-06-13" version="1">MPLRRRRLVFLLFLALACSGRGAGAETTSGNATSPPLSCSELSRVCTAFLAFPAAGAAANASVLESMFDASPGDLTADAAASPGYAFVRKNCSCLASRTYLANTTYTVPSAAPPNATAAEVAAAAYAGLAVPPPGGAAHRPPRPSAVVALHLLCGCSSGPWNYLLSYVGADGDTVESLSSRFGASMDAIEATNDMAGPDPINTGKVYYIPLNSVPGQPYSEKSPAPAFAPSPQNTLSEISDHHPARFPYGWVIGSMGVAFALIAIALLVVVMCKFFQYNHQVPDNQRKSPDQPMSHDFRLLKSGSFCYDSGRYLCCQFGNAKKSRKGAGDHRINVPKGMVVDVFDREKPIVFTYEEILESTDLFSDANLLGHGTYGSVYYGVLRDQEVAIKKMTATNAKEFIVEMKVLCKVHHASLVELIGYAASKDELFLVYEYSQKGSLKNHLHDPQSKGYTSLSWIYRVQIALDAARGLEYIHEHTKDHYVHRDIKSSNILLDGSFRAKISDFGLAKLAVRSNDAEASVTKVVGTFGYLAPEYLRDGLATSKCDVYAFGVVLFELISGKEAITKTDAISAGSNSERRSLASVMLTALRNCHNPACVGTLKDCIDPNLMELYPHDCIYQMAMLAKQCVDEDPVLRPDMKQAVITLSQILLSSIEWEATLGGNSQVFSGLVAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1HS52</accession>
    <name>I1HS52_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dymeclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100845902</name>
        <name evidence="5" type="ORF">BRADI_2g51430v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dymeclin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569930.1">
        <property type="nucleotide sequence ID" value="XM_003569882.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HS52"/>
    <dbReference type="STRING" id="15368.I1HS52"/>
    <dbReference type="EnsemblPlants" id="KQK09996">
        <property type="protein sequence ID" value="KQK09996"/>
        <property type="gene ID" value="BRADI_2g51430v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845902"/>
    <dbReference type="Gramene" id="KQK09996">
        <property type="protein sequence ID" value="KQK09996"/>
        <property type="gene ID" value="BRADI_2g51430v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845902"/>
    <dbReference type="eggNOG" id="KOG2225">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013309_1_0_1"/>
    <dbReference type="OMA" id="NFVKRPR"/>
    <dbReference type="OrthoDB" id="10253409at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HS52">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019142">
        <property type="entry name" value="Dymeclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12895">
        <property type="entry name" value="DYMECLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12895:SF9">
        <property type="entry name" value="DYMECLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09742">
        <property type="entry name" value="Dymeclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK09996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="714" mass="80782" checksum="2CA8FAB261E31C5C" modified="2012-06-13" version="1">MGAAPSTPRLGEVGAAPPGAAEQMFAALVGDKAYPISSEFWKQLFELPLTLQWPRERVLQACHAFAQNNYNTKHLTKILIHLVWCLQECTSASSVSYGVYRKAINAAYISSIFLKFIIENAKADNWQELCLHIDKDEKGLENFPSDQTVEYFLMKGVLNYIGSVDVSPESCYLHHELLNLMLVLMSTQLCSGPSPEPKDVHPFIDAAMLQESTTVGSVVQKLLLNFVRRPQIPLNGSHPVFSDNGGPGVLQRVGSAAANLVLLPYYTFNYLVNASAEGATSQLADNSLLVLLILIHYRKCISMTESIPIDNIYMSDSNTNIKDAPSFHENPYCKALNNAKDIQFDRADVEGNAQNGPVVRLSFASLFDALGTCLKDESSVLLLYSLVHGNCDFQEYVLVRTDLDTLLMPILEMLYNASRKTSNQIYMLLIILLILSQDSTFNASVHKLVLPSVPWYQERLMHQTSLGSLMVVILIRTIKYNLSKLRDVYLHTNCLAILANMGPHVHRLSAYASQRLVSLFDMLSRKYAKLAELKNDKASKIISDQIEPDNISDDTSTELHIYTDFLRIVLEIVNAILTYALPRNPEVVYAILHRQEVFQPFKNHPRFNELLENIYTVLDFFNSRMDMQQLDGEWSVDKVLELINKNCRSWRGEGMKMFTQLRFTYEQESHPEEFFIPYAWRLILSRGFSFNPGAINLFPVEIHVDDAQPGEQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSZ1</accession>
    <name>I1HSZ1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g53750v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSZ1"/>
    <dbReference type="FunCoup" id="I1HSZ1">
        <property type="interactions" value="644"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HSZ1"/>
    <dbReference type="EnsemblPlants" id="KQK10384">
        <property type="protein sequence ID" value="KQK10384"/>
        <property type="gene ID" value="BRADI_2g53750v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10384">
        <property type="protein sequence ID" value="KQK10384"/>
        <property type="gene ID" value="BRADI_2g53750v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845274"/>
    <dbReference type="eggNOG" id="ENOG502SY0N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134973_3_2_1"/>
    <dbReference type="OMA" id="MHCEECI"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF304">
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    </dbReference>
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        <property type="entry name" value="HMA, heavy metal-associated domain"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK10384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="69" mass="7786" checksum="685A14D68F3B3801" modified="2012-06-13" version="1">MAVVELKVGMHCDRCIKSIKKAIKTIDDMESYQLEKETNKVTVTGNITPEEVVKALQKIGKTVTYWGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1HW22</accession>
    <name>I1HW22_BRADI</name>
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC104583398</name>
        <name evidence="3" type="ORF">BRADI_3g00580v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ92770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010235352.3"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQJ92770">
        <property type="protein sequence ID" value="KQJ92770"/>
        <property type="gene ID" value="BRADI_3g00580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583398"/>
    <dbReference type="Gramene" id="KQJ92770">
        <property type="protein sequence ID" value="KQJ92770"/>
        <property type="gene ID" value="BRADI_3g00580v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583398"/>
    <dbReference type="eggNOG" id="ENOG502RRNW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017945_2_2_1"/>
    <dbReference type="OMA" id="NYSRNHA"/>
    <dbReference type="OrthoDB" id="605595at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AT5G49610-like_b-prop"/>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OS07G0120400 PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF23635">
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    </dbReference>
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            <begin position="20"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="F-box protein AT5G49610-like beta-propeller" evidence="2">
        <location>
            <begin position="121"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ92770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="409" mass="45361" checksum="95DF3E907FAB5A07" modified="2012-06-13" version="1">MSRRRHSTPAPATLAPLDDEDLLREILLRLPPSPSSLPRASLVCKLWRGILSDPGFGRRFRKHHRIPPLLGCFVKDLGMAPVFSPLLDPPDRIPAPLPENLADPSSWSSTHRRLSFSFHGCRHGIALLLDHRRNEAVLWDPLTGVQRRVCFPPGLGFAGGAQSGIVWNAAVLRTASADDHDDCRLTPFKLVLACRDDKFTKEFVCLYESNTGVWGDVISITTTDRIGLKSPSVLVGNTLCWLVSGGGILEFDFERQTLVVIDEPTDLHATDPYPGMIHRSFQILRGEDGGLGLAVLSDSEQSIQLWARKSDSDGVGSWVLRKTVQLDERFSRPEGTQKRLWMPGYDEDTNAIFLSLDSDDFMLQLESMQFTCLHRNGLEIFRTYHPYAGFYTAGRGIAGGDGGAENVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1HWF1</accession>
    <name>I1HWF1_BRADI</name>
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        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g01667v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HWF1"/>
    <dbReference type="EnsemblPlants" id="PNT65740">
        <property type="protein sequence ID" value="PNT65740"/>
        <property type="gene ID" value="BRADI_3g01667v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65740">
        <property type="protein sequence ID" value="PNT65740"/>
        <property type="gene ID" value="BRADI_3g01667v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="I1HWF1"/>
    <dbReference type="OrthoDB" id="1913924at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HWF1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAH_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35992">
        <property type="entry name" value="CYTOMATRIX PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT65740"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="523" mass="58732" checksum="185AB84C32CB070A" modified="2012-06-13" version="1">MAVHLRPGGGDAEQEPGASRGARRRRARLETASKAQRETGEAMLKLQRRVEELQAAAREKDAEVGRLRAEAAGARTKLQEAHQESESCANLSQNTAEHDIASNLGTELRKLKQAYEILSSKKDKEVSALLSERDFVCDQMSIMEQDFAALLKKLEAAQATESAQKLQQNINELQVLAEKKDDEISRLRAKAVSAKEKIDYLQLLVKEKDDEIERLKGGLPETIPKRNKDISETHKRSRNEGPAVWTESKNSCPRTMIEEDFASEIEMAEKHGQAETSKKPKYASSLSNSGNGGDGDEQSGHDEDGDEQSGGDKDDELIGTDEDGDEQSGTDEDSDEQSGTDEDGNEQSGSDDGSEDVDEQNREDERIWKGQSTMKCSLRKYRNGQSTMKSTGNWALFVSDVNDILADRPDRHKRFIDFLHTFRHKKPRIDSVASTMECVLDGHPDLVRRFNKNRIEVKTKWRPVPGGQRSHQGQRGGEVGKEEKPIFQTKQIVEPLNQTLPKKRGRPRARSWYSWKRGEAQVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HX12</accession>
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    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_3g03340v3</name>
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    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT65832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HX12"/>
    <dbReference type="FunCoup" id="I1HX12">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HX12"/>
    <dbReference type="EnsemblPlants" id="PNT65832">
        <property type="protein sequence ID" value="PNT65832"/>
        <property type="gene ID" value="BRADI_3g03340v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65832">
        <property type="protein sequence ID" value="PNT65832"/>
        <property type="gene ID" value="BRADI_3g03340v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2782">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038422_3_1_1"/>
    <dbReference type="InParanoid" id="I1HX12"/>
    <dbReference type="OMA" id="NPAKRTF"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.170:FF:000004">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000239">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="entry name" value="Putative methyltransferase TM0872, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00006">
        <property type="entry name" value="16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265:SF0">
        <property type="entry name" value="12S RRNA N4-METHYLCYTIDINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="S-ADENOSYL-METHYLTRANSFERASE MRAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="Putative methyltransferase TM0872, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="PNT65832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="430" mass="47849" checksum="9614C5EFE99A0757" modified="2012-06-13" version="1">MAMAARRLLFSSSHLRPLAAAPHRRKHDAVSCKSTGKARTGTSTKPKDSGAGKWRPQRRALEEHLKRRTRSAGAFDADLYRRHSHSHHVPVLLGEVLAAFRRPLPLRSFVDCTLGAAGHSLAMMEAHPEMELHIGMDVDPSALEIGQRHVEAFLASRATDGGDSLQGVLRAYTHVKNFKYIKHVLGGVDESLLADSSGVDGILIDLGMSSMQVNRSDRGFSVLHDGPLDMRMDPKATLTAEDILNSWPELEVGRILRDYGEESNWQSLQRRIVKARQAGGLHSTGELVKLIQRSCTISGGRQGWIKTATRVFQALRIAVNDELRILEDTLHSCFDCLATDGRLAVISFHSLEDRIVKQTFLELIHGDGVDDGDEDLVCTDIDDEDEPWFKQRVKGMNGIILTKRPITPSQEEEKLNQRCRSAKLRVIQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1HX53</accession>
    <name>I1HX53_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100839729</name>
        <name evidence="12" type="ORF">BRADI_3g03690v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574558.1">
        <property type="nucleotide sequence ID" value="XM_003574510.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574559.1">
        <property type="nucleotide sequence ID" value="XM_003574511.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316620.1">
        <property type="nucleotide sequence ID" value="XM_024460852.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316621.1">
        <property type="nucleotide sequence ID" value="XM_024460853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HX53"/>
    <dbReference type="STRING" id="15368.I1HX53"/>
    <dbReference type="EnsemblPlants" id="KQJ93292">
        <property type="protein sequence ID" value="KQJ93292"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93293">
        <property type="protein sequence ID" value="KQJ93293"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93294">
        <property type="protein sequence ID" value="KQJ93294"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93295">
        <property type="protein sequence ID" value="KQJ93295"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93296">
        <property type="protein sequence ID" value="KQJ93296"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ93297">
        <property type="protein sequence ID" value="KQJ93297"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839729"/>
    <dbReference type="Gramene" id="KQJ93292">
        <property type="protein sequence ID" value="KQJ93292"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93293">
        <property type="protein sequence ID" value="KQJ93293"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93294">
        <property type="protein sequence ID" value="KQJ93294"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93295">
        <property type="protein sequence ID" value="KQJ93295"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93296">
        <property type="protein sequence ID" value="KQJ93296"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ93297">
        <property type="protein sequence ID" value="KQJ93297"/>
        <property type="gene ID" value="BRADI_3g03690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839729"/>
    <dbReference type="eggNOG" id="KOG0213">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002242_0_1_1"/>
    <dbReference type="OMA" id="LVMNYVW"/>
    <dbReference type="OrthoDB" id="438939at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000066">
        <property type="entry name" value="Splicing factor 3B subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000069">
        <property type="entry name" value="Splicing factor 3B subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000073">
        <property type="entry name" value="Splicing factor 3b, subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015016">
        <property type="entry name" value="SF3b_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038737">
        <property type="entry name" value="SF3b_su1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12097">
        <property type="entry name" value="SPLICING FACTOR 3B, SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08920">
        <property type="entry name" value="SF3b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="660"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="11">
        <location>
            <begin position="856"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="172"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ93292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1279" mass="142009" checksum="F350C40B9A779FB2" modified="2012-06-13" version="1">MDGIDAELARAQDERRKLEEALEAGAPMAVSSVTFDTDLYGGGGSDPNRFAGYDTSIPASEDDAPEDVHAEPAANPAARRLASYTGHAVAAADIPRSDDDGMPVKKSQRIIDREDDYRRRRLDRIISPERHDAFAAGEATPDPSVRTYVDAMRENKVQQEKEYVLREIAKKKKEEEEKAKEKKAAPEPVPAATKRRNRWDQSQDGDTAAAGAKKAKTASDWDAPDATPGIGRWDATPGRIGDATPSVRRNRWDETPTPGRMADADATPAAGGITPGATPSGAWDATPKLPGGLVTPTPKKQRSRWDETPASMGSATPGGTAATPANYTPGVTPFGAENLATPTPGHLARGPITPEQYQLMRWERDIEERNRPLTDEELDSMFPQEGYKILEPPASYQPIRTPARKLLATPTPLGTPLYAIPEENRGQQYDVPKEMVGLPLMKPEDYQYFGTLLNEDEEEQLTPEEQKERKIMKLLLKVKNGTPPQRKTALRQLTDKAREFGAGPLFNKILPLLMQPTLEDQERHLLVKVIDRVLYKLDELVRPFVHKILVVIEPLLIDEDYYARVEGREIISNLSKAAGLATMIAAMRPDIDNIDEYVRNTTARAFSVVASALGIPALLPFLKAVCQSKKSWQARHTGIKIVQQIAILMGCAVLPHLKNLVEIIEHGLSDENQKVRTITALSLAALAEAAAPYGIESFDSVLKPLWKGIRSHRGKVLAAFLKAIGFIIPLMDALYASYYTKEVMQVLIREFQSPDEEMKKIVLKVVKQCVSTEGVEADYIRSDILPDFFKHFWVRRMALDRRNYKQLVETTVEMANKVGVTGIVGKIVEDLKDESEPYRRMVMETIEKVVANLGASDIEPRLEELLIDGILYAFQEQTSDDANVMLNGFGAVVNALGQRVKPYLPQICGTIKWRLNNKSAKVRQQAADLISRIAIVMKQCQEEQLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMTKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEFVPAREWMRICFELLEMLKAHKKGIRRATVNTFGYIAKAIGPQDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTAASAVKHMALGVAGLGCEDALVHLLNYVWPNIFETSPHVINAVMEAIEGMRVALGAAVVLNYCLQGLFHPARKVREVYWKIYNSLYIGAQDALVASYPALGDDGDNIFSRPELAMFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HXM3</accession>
    <name>I1HXM3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">4-hydroxyphenylpyruvate dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100841334</name>
        <name evidence="18" type="ORF">BRADI_3g05060v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="15">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Cofactor biosynthesis; prenylquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the 4HPPD family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570001.1">
        <property type="nucleotide sequence ID" value="XM_003569953.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HXM3"/>
    <dbReference type="FunCoup" id="I1HXM3">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HXM3"/>
    <dbReference type="EnsemblPlants" id="KQJ93508">
        <property type="protein sequence ID" value="KQJ93508"/>
        <property type="gene ID" value="BRADI_3g05060v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841334"/>
    <dbReference type="Gramene" id="KQJ93508">
        <property type="protein sequence ID" value="KQJ93508"/>
        <property type="gene ID" value="BRADI_3g05060v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841334"/>
    <dbReference type="eggNOG" id="KOG0638">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034004_1_1_1"/>
    <dbReference type="OMA" id="DPFPVKG"/>
    <dbReference type="OrthoDB" id="414569at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003868">
        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08342">
        <property type="entry name" value="HPPD_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000013">
        <property type="entry name" value="4-hydroxyphenylpyruvate dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000025">
        <property type="entry name" value="4-hydroxyphenylpyruvate dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041736">
        <property type="entry name" value="4OHPhenylPyrv_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01263">
        <property type="entry name" value="4HPPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959:SF1">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009283">
        <property type="entry name" value="HPP_dOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="11 15" id="KW-0408">Iron</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="VOC" evidence="17">
        <location>
            <begin position="40"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="17">
        <location>
            <begin position="210"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR009283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQJ93508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="436" mass="46328" checksum="A359BFD633804FF0" modified="2012-06-13" version="1">MPPPATTAAPAAAAVTPEHARPPRRVARVNPRSDRFSALSFHHVELWCADAASAAGRFSFALGAPPAARSDLSTGNSAHASILLRSGSLAFLFTAPYAPSPASDSAASIPSFSASAARQFTADHGGLAVRAVALRVSSASDAFHASVSAGARPSFPPADLGQGFALAEVELYGDVVLRFISHPDENTEIPFLPGFESVSNPGASTYGLTRFDHVVGNVPSLAPVAAYIAGFTGFHEFAEFTAEDVGTADSGLNSVVLANNSERVLLPLNEPVHGTKRRSQIQTYLDHHGGPGVQHIALASDDVLRTLREMRARSAMGGFEFLAPPPPNYYDGVRRRAGDVLSEAQIKECQELGVLVDRDDQGVLLQIFTKPVGDRPTLFLEMIQRIGCMEKDEIGQEQQKGGCGGFGKGNFSELFRSIEEYEKSLEAKQSAVVQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1I0D0</accession>
    <name>I1I0D0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100830124</name>
        <name evidence="8" type="ORF">BRADI_3g13330v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32955"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the PI(3,5)P2 regulatory complex at least composed of ATG18, SAC/FIG4, FAB1 and VAC14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573274.1">
        <property type="nucleotide sequence ID" value="XM_003573226.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I0D0"/>
    <dbReference type="FunCoup" id="I1I0D0">
        <property type="interactions" value="2893"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I0D0"/>
    <dbReference type="EnsemblPlants" id="KQJ94805">
        <property type="protein sequence ID" value="KQJ94805"/>
        <property type="gene ID" value="BRADI_3g13330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830124"/>
    <dbReference type="Gramene" id="KQJ94805">
        <property type="protein sequence ID" value="KQJ94805"/>
        <property type="gene ID" value="BRADI_3g13330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830124"/>
    <dbReference type="eggNOG" id="KOG1888">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_4_0_1"/>
    <dbReference type="OMA" id="KRKCCAH"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I0D0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009832">
        <property type="term" value="P:plant-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009826">
        <property type="term" value="P:unidimensional cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="7">
        <location>
            <begin position="154"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="638"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="638"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ94805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="899" mass="100720" checksum="7B37946789D6EBC0" modified="2012-06-13" version="1">MAAETDGPTAAAATLEKFRLYETRARFYLIGSSREKRWFRVLKIDRSEKSELHLSEDPVWYSQQEVKSLLQRIAEGNRSTGGLTFVTKAYGIAGCIKFLESYYLILVTKRRQIGCICGHAIYCIDESQMITIPHTSVQTDVATSKNELRYKKLLASVDLTKDFFYSYTYPIMQSLQQNVTSAGTKELPYENLFVWNTFLTEPIRSMCKNTLWNVALVHGHFKQVKLSIFGRELNVVLISRRSRHFAGTRYLKRGVNDHGKVANDVETEQIVFEEEAGSWKGRMSAIVQMRGSIPLFWSQEAGRLSPKPDIFVQRYDPTYEATKLHFDDLAQRYGQPIIILNLIKTVEKRPREMMLRREFFNAVGYLNQNVPEEKKLRFIHWDFHKFAKSKSANVLGVLGGVASEALDLTGFYYSGKPKVQKRRPIQLSRTSTARDGSIDVRASSGDLLRLSSNADALGSTASQDMRTEDSKQEPLGDAPCYQTGVLRTNCIDCLDRTNVAQYAYGLAALGRQLHAMGLTAVSKIHPDSSIASALMEMYQSMGDALAHQYGGSAAHNTVFPERQGKWKATTQSREFLKSIKRYYSNAYTDGEKQDAINLFLGYFQPQEGKPALWELDTDYYLHVTTAGDDLVSDSYRLSSSPANNASGGSEAALSPRSTLSPVPACKEDFSRMKLTSFDKLIERTCSSIRSVRLHCDADLRPSGGVGTSGMAPDAAEIQLKSPNWLFGQRKHAETVPTAKVIPVESANDGNKDDTNAPLCGELNWLSSEGSCEEDNFRRYLALTTADVENGWYGGELLYDQDENSGAYKHYAELCQGPVMDPFEHDPEKERHYAEALSVDIEITDEAWVESEMKAALNDYQIIGSDLSIIPSCGALAEDPSQLTRWIIGDEKLRAVATTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1I0H5</accession>
    <name>I1I0H5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF4005 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100846416</name>
        <name evidence="9" type="ORF">BRADI_3g13710v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Binds to multiple calmodulin (CaM) in the presence of Ca(2+) and CaM-like proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IQD family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571316.1">
        <property type="nucleotide sequence ID" value="XM_003571268.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I0H5"/>
    <dbReference type="FunCoup" id="I1I0H5">
        <property type="interactions" value="389"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I0H5"/>
    <dbReference type="EnsemblPlants" id="KQJ94865">
        <property type="protein sequence ID" value="KQJ94865"/>
        <property type="gene ID" value="BRADI_3g13710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846416"/>
    <dbReference type="Gramene" id="KQJ94865">
        <property type="protein sequence ID" value="KQJ94865"/>
        <property type="gene ID" value="BRADI_3g13710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846416"/>
    <dbReference type="eggNOG" id="ENOG502R300">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024547_1_0_1"/>
    <dbReference type="OMA" id="TDHRDSI"/>
    <dbReference type="OrthoDB" id="776767at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I0H5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000062">
        <property type="entry name" value="IQ-domain 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025064">
        <property type="entry name" value="DUF4005"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF6">
        <property type="entry name" value="PROTEIN IQ-DOMAIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13178">
        <property type="entry name" value="DUF4005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DUF4005" evidence="8">
        <location>
            <begin position="432"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="22"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="345"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="521"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="347"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="436"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="549"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ94865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="583" mass="63222" checksum="3D7CEC254242A572" modified="2012-06-13" version="1">MGNNKKGSGSSSSSTSWLTAVKRAFRSPSSKEDSPASKSARLRDDDRGPNSEDDKSKRSERRRWLFRRSSSPSPSPAPPAPPPEQSHQQRQPRSAAPAPAVSDEQRHAIALAVATAATAEAAVATAHAAAEVVRLTRPAATSNGNNSCYVREHYAAILIQTAFRGYLARRALRALKGLVKLQALVRGHNVRKQANMTLRCMQALVRVQARVRDQRMRLSQDSLSFSGAGHGANGNGSSKSSYSVDTSAFWDSKYTHDYADRRSMERSRDGSSFAAAEDWDDRPRTIEEIQAMLQTRKDAALKRERALSYAFSHQIWRNPAPSVEEEMDGEQPRWADRWMASRASFDTNRSSRTAAAAAAPGRASTDHRAHQQHVKTLEMDTSRPFSYSTPRRQQQQQQQAPAHGSGSPMHHRATPSPGKARPPVQVRSASPRANGGSYTPSLHSQRLQHASSAAVPNYMAATESAKARVRSHSAPRQRPATPERGGADRYLQQVQHAGGGAFSVSSAAASSAKKRLSFPAAADTYSGGGGGYAQSLRSPSFKSAAGRFSSEQRSTVSSLSCAESLGGEPVSPSSTTDLRRWLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1I629</accession>
    <name>I1I629_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100826560</name>
        <name evidence="8" type="ORF">BRADI_3g33120v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the expansin family. Expansin B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234990.1">
        <property type="nucleotide sequence ID" value="XM_010236688.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I629"/>
    <dbReference type="FunCoup" id="I1I629">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ97764">
        <property type="protein sequence ID" value="KQJ97764"/>
        <property type="gene ID" value="BRADI_3g33120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826560"/>
    <dbReference type="Gramene" id="KQJ97764">
        <property type="protein sequence ID" value="KQJ97764"/>
        <property type="gene ID" value="BRADI_3g33120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826560"/>
    <dbReference type="eggNOG" id="ENOG502QRTE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027462_1_0_1"/>
    <dbReference type="OMA" id="HANRDGN"/>
    <dbReference type="OrthoDB" id="606990at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22275">
        <property type="entry name" value="DPBB_EXPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005795">
        <property type="entry name" value="LolPI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF19">
        <property type="entry name" value="EXPANSIN-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00829">
        <property type="entry name" value="LOLP1ALLERGN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095098" description="Expansin" evidence="5">
        <location>
            <begin position="26"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="6">
        <location>
            <begin position="60"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="7">
        <location>
            <begin position="181"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ97764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="29827" checksum="834770BBFDBB946B" modified="2012-06-13" version="1">MAGVSTNAVALVALLSAVLVSTGHSQQVNYDTARSYNSGWLPAKATWYGAPTGAGPDDNGGACGYKDTNQYPYSSMISCGNEPLFMGGAGCGTCYQIRCNYANNPACSGQPRLVTITDMNYYPVAKYHFDLSGTAFGAMANNGQNDRLRHAGIIDMQFRRVPCNYPGMNVNFHVERGSNPNYLAVLVQHANRDGNVVLMEIMESRYGRPTGQWTAMTRSWGAIWRRDTNYPMQGPFSLRIRSESGSTLVANQAIPADWKPNARYWSNIQYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6L4</accession>
    <name>I1I6L4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleobase-ascorbate transporter 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100845314</name>
        <name evidence="7" type="ORF">BRADI_3g34660v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT67992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574409.1">
        <property type="nucleotide sequence ID" value="XM_003574361.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I6L4"/>
    <dbReference type="STRING" id="15368.I1I6L4"/>
    <dbReference type="EnsemblPlants" id="KQJ98047">
        <property type="protein sequence ID" value="KQJ98047"/>
        <property type="gene ID" value="BRADI_3g34660v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT67992">
        <property type="protein sequence ID" value="PNT67992"/>
        <property type="gene ID" value="BRADI_3g34660v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845314"/>
    <dbReference type="Gramene" id="KQJ98047">
        <property type="protein sequence ID" value="KQJ98047"/>
        <property type="gene ID" value="BRADI_3g34660v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT67992">
        <property type="protein sequence ID" value="PNT67992"/>
        <property type="gene ID" value="BRADI_3g34660v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845314"/>
    <dbReference type="eggNOG" id="KOG1292">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017959_5_3_1"/>
    <dbReference type="OMA" id="FMEHIGD"/>
    <dbReference type="OrthoDB" id="1641903at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037981">
        <property type="entry name" value="NCS2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ98047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="57663" checksum="DB9607C2FFFE7499" modified="2012-06-13" version="1">MADMKPEEVSHPPMDQLQGLEYCIDSNPSWGEAVALGFQHYILCLGTAVMIPTFLVPLMGGNAHDKAKVVQTMLFVTGINTLLQTLFGTRLPTIIGGSYAFVIPVISIIRDPSLTQIADDHTRFIMTMRATQGALIISSCIQIVLGYSQLWGICSRFFSPLGMVPVVALVGLGLFERGFPLMGRCVEIGLPMLVLFVALSLYLKHVQVRHLPILERFSLVISIALVWVYAHILTVSGAYKHSSLATQVNCRTDRANLIASADWISIPYPLQWGPPTFSADHAFGMMSAVMVSLVESTGAFKAAARLASATPPPPYVLSRGIGWQGIGLLFDGLFGTVAGSTVSVENVGFLGSTRIGSRRVIQISAGFMIFFSILGRFGGLFASIPFTIFAAIYCVMFGYVGAVGLSFMQFTNMNSMRSLFIIGISLFLGMSIPEYFFRYTMSSQQGPAHTRAGWFNDYINTIFSSPPTVALIIAVALDNTLEVRDAARDRGMQWWERFRTFRGDSRNEEFYTLPFNLNRFFPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I1I718</accession>
    <name>I1I718_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100841963</name>
        <name evidence="14" type="ORF">BRADI_3g35860v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574483.1">
        <property type="nucleotide sequence ID" value="XM_003574435.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I718"/>
    <dbReference type="FunCoup" id="I1I718">
        <property type="interactions" value="2317"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I718"/>
    <dbReference type="EnsemblPlants" id="KQJ98274">
        <property type="protein sequence ID" value="KQJ98274"/>
        <property type="gene ID" value="BRADI_3g35860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841963"/>
    <dbReference type="Gramene" id="KQJ98274">
        <property type="protein sequence ID" value="KQJ98274"/>
        <property type="gene ID" value="BRADI_3g35860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841963"/>
    <dbReference type="eggNOG" id="KOG0337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_5_2_1"/>
    <dbReference type="OMA" id="EDQFGMM"/>
    <dbReference type="OrthoDB" id="10261375at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012541">
        <property type="entry name" value="DBP10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF8">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01123">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="55"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="86"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="283"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="691"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="55"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="769"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="787"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="806"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="839"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ98274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="851" mass="94802" checksum="DBBE6428BF75735C" modified="2012-06-13" version="1">MARLTPSTSSSKSKPSMDGAASSGEHRPRRRPQMKSKAAGKKKAAQAAHKKPKSGGFESMGLCEEVYRGVRHKGYRVPTPIQRKAMPLILAGLDVAAMARTGSGKTAAFLVPMLQRLRRRDPGAGIRALILSPTRDLAMQTLKFAHQLGKFTGLKTEAIVGGGSIDSQFEILADNPDIIIATPGRLVHILTMVNDLSLRSVEYVVFDEADSLFSLGFAEQLHTILQKLSDTRQTLLFSATMPKALAEFAKAGLRDPQVLRLDVDKKISPDLKLVFLTLRQEEKLAALLYLVRERISSEEQTMIFVSTKYHVEFLNILFREEGLEPSLSYGAMDQEARNIHISKFRAKKTMLLIVTDVAARGLDIPLLDNVVNWDFPAKPKLFVHRVGRVARQGRTGTAYSFVTSEDMPFLLDLHLFLSKPLRPAPTEEELLKDMDGMNLKIDQSLANGETVYGRFPQTIIDLCSDGVKEVINGCTDLIALEKPCANAFRLYLKTRAMPSKESIRRAKDFPREGLHPIFRDVLRSDEISALAFSERLKSFRPKQTILEAEGEAAKSRSSKGSNQWLDVMKKKREVHEGIINLVHEKNSGDLATKEEEAENISNWETKEVCGKKRKSQSFRDEDYYISSVPQNQHSEAGLSVKGNEGFVQDRLDAAVLDLVDDETSGMQSQKTRYHWMKNKFVKLNSGDRVTASGKIKTERTAKSKAASQDIYKKWQQKSHRAINSGGKDGDFREGAPSTPGGYQRGNRRHPAAGRGRWSIPNADVPSEIRNPQQMQKGRQQKAMQNMRMKEKSAKDGKFPGKFQKNKRPDGSGRDGKFQKNRKPDGKGSRKGNGNGFGKAKGKGKPKGKGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1I9I9</accession>
    <name>I1I9I9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carboxypeptidase</fullName>
            <ecNumber evidence="2">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100837185</name>
        <name evidence="3" type="ORF">BRADI_3g43020v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572508.1">
        <property type="nucleotide sequence ID" value="XM_003572460.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I9I9"/>
    <dbReference type="FunCoup" id="I1I9I9">
        <property type="interactions" value="417"/>
    </dbReference>
    <dbReference type="MEROPS" id="S10.017"/>
    <dbReference type="EnsemblPlants" id="KQJ99393">
        <property type="protein sequence ID" value="KQJ99393"/>
        <property type="gene ID" value="BRADI_3g43020v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837185"/>
    <dbReference type="Gramene" id="KQJ99393">
        <property type="protein sequence ID" value="KQJ99393"/>
        <property type="gene ID" value="BRADI_3g43020v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837185"/>
    <dbReference type="eggNOG" id="KOG1283">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008523_1_0_1"/>
    <dbReference type="OMA" id="QEPKEVW"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000123">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF498">
        <property type="entry name" value="OS03G0393066 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982787" description="Carboxypeptidase" evidence="2">
        <location>
            <begin position="29"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ99393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="471" mass="50784" checksum="588A0E7D2A1202E4" modified="2012-06-13" version="1">MAAPGRLLLLSCIAALLLLVLAPNGAAAAGTADGSEEWGYVQVRPKAHMFWWLYRSPHRVDNASTPWPTVLWLQGGPGASGVGYGNFEEIGPLDVALKPRNSTWLNKADLLFVDNPVGTGFSFVEAGNTTLLAHSDAQTATDLTALLVKLYGGGSGAPLKQGSPLYIVAESYGGKFAVTTALAALKAIGQGQLKATLAGVALGDSWISPEDFVLSWGPLLYQMSRLDENGLQNCDKIAQKIKAQLKANQYTEAEASWEELENAISALTNAIDFYNILKDSSSSDSSAAAPLTASKRQAWLRRKSTRPGRYLRSLMAAEEGGLQGLMDTRIKAKLGIIPANFTWGQQDDAVFDALKPDFMKPRIHEVDELLKLGVNVTIYTGQLDLICATKGTLDWVQKLKWEGLKNFTAAPRKPIYCNGAEAAGTEGTQAFLKSYKNLNFYWIMGAGHMVPVDNPCTALKMLGDITQSPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1IEB2</accession>
    <name>I1IEB2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100827890</name>
        <name evidence="3" type="ORF">BRADI_3g56370v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570466.1">
        <property type="nucleotide sequence ID" value="XM_003570418.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IEB2"/>
    <dbReference type="FunCoup" id="I1IEB2">
        <property type="interactions" value="1784"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01517">
        <property type="protein sequence ID" value="KQK01517"/>
        <property type="gene ID" value="BRADI_3g56370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827890"/>
    <dbReference type="Gramene" id="KQK01517">
        <property type="protein sequence ID" value="KQK01517"/>
        <property type="gene ID" value="BRADI_3g56370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827890"/>
    <dbReference type="eggNOG" id="ENOG502QR86">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080570_0_0_1"/>
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    <dbReference type="OrthoDB" id="47923at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IEB2">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
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        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31923">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31923:SF1">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
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    </dbReference>
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        <property type="entry name" value="BSD domain-like"/>
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        <property type="entry name" value="BSD"/>
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            <begin position="150"/>
            <end position="195"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="214"/>
            <end position="305"/>
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    </feature>
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            <begin position="218"/>
            <end position="232"/>
        </location>
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        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="305" mass="34146" checksum="8878F099F1B16353" modified="2012-06-13" version="1">MDLWERARSFAGEAAKRSQELSLEAAKRSSVLVSETAKKSKEIFSETATKSREIAAEATKQADLLAGQIMHLASDIPVPSIPPIPSIPPIPSAAESEPDAAELERYGITEDLREFVKGMTISTFRDFPLQEEPEMSDVPTVSNVRQDLNEWQARHATLVLSAVKEISKFRYELCPRYMKERKFWRIYFLLVNNYIALYETKYFEELKVKAEEEKRISQQEVSQTSQATTSEQTETKVQSKTSTSSNREHDLDVFLLGDLGSDDEGPDGGDEGLDDDFDKIDGASGLESDEDEKKSATGNSETAKE</sequence>
</entry>
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        <lineage>
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            <taxon>Spermatophyta</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ86143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003579201.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IH54"/>
    <dbReference type="STRING" id="15368.I1IH54"/>
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        <property type="gene ID" value="BRADI_4g03590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830680"/>
    <dbReference type="Gramene" id="KQJ86143">
        <property type="protein sequence ID" value="KQJ86143"/>
        <property type="gene ID" value="BRADI_4g03590v3"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_038016_1_0_1"/>
    <dbReference type="OMA" id="ERSEHYF"/>
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        <property type="component" value="Chromosome 4"/>
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    </dbReference>
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    </evidence>
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            <dbReference type="EnsemblPlants" id="KQJ86143"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="33783" checksum="20B293DCF56500B4" modified="2012-06-13" version="1">MQPPKKPARVDTAEIKSQLVKKLGPQRAELYIHSLNKFLGFQLDKSEFDKICVAALGKENIKLHNSLVRSILSNAFMGLGPPPSRQTPTGNSQTSAVTNGPLGNGVPLPRRVRPLGNRDRRCTDKPSPLGKSPLGNPGAAEFVSVEDGEEVDQDRGSPLCVQSQSPIRAPLGVATVQQPQFSALRPSDVCYINGELPDTECLSKILQDKLDAEGLSMPIECANLLNSGLNAYIRQLLKSCLDVAKARGNTMRAHQANGNASSSSAALNGGLNNGSVSDSGRVYQASLVDLSTAVQSNPKLLGCDSVKQKDKIASHLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I1IM39</accession>
    <name>I1IM39_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA replication licensing factor MCM2-like winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g20640v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IM39"/>
    <dbReference type="STRING" id="15368.I1IM39"/>
    <dbReference type="EnsemblPlants" id="KQJ88707">
        <property type="protein sequence ID" value="KQJ88707"/>
        <property type="gene ID" value="BRADI_4g20640v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88707">
        <property type="protein sequence ID" value="KQJ88707"/>
        <property type="gene ID" value="BRADI_4g20640v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0477">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1273784_0_0_1"/>
    <dbReference type="InParanoid" id="I1IM39"/>
    <dbReference type="OrthoDB" id="844at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059098">
        <property type="entry name" value="WHD_MCM2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23669">
        <property type="entry name" value="WHD_MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="138"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ88707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="217" mass="23316" checksum="C17FB3143A49AE7C" modified="2012-06-13" version="1">MDRRKGSGRRDANGGLTEATALRLSFEDADEVAEEVEESAAAEGEAAAAEVIGSKKTRADPSGVSSLAPPSPRAPGVRRTSSSRPRLSATCVSSSRERLPSSSTAHAACCAAQTAAPLVAAGACLSAQSFHKYMTYKKDYNELLLLLLRTLVKDVLHFEEIMSGSTTRLTHIEVKVDDLGQEFEIYDLKPFFSSSHFSDNNFVLDEGRGITKHPIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1INB3</accession>
    <name>I1INB3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g24857v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INB3"/>
    <dbReference type="FunCoup" id="I1INB3">
        <property type="interactions" value="493"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1INB3"/>
    <dbReference type="EnsemblPlants" id="KQJ89319">
        <property type="protein sequence ID" value="KQJ89319"/>
        <property type="gene ID" value="BRADI_4g24857v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ89319">
        <property type="protein sequence ID" value="KQJ89319"/>
        <property type="gene ID" value="BRADI_4g24857v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001090_4_0_1"/>
    <dbReference type="InParanoid" id="I1INB3"/>
    <dbReference type="OMA" id="SHILFFQ"/>
    <dbReference type="OrthoDB" id="675568at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1INB3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377:SF66">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377">
        <property type="entry name" value="OS10G0134700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ89319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="482" mass="55150" checksum="481D809596537D46" modified="2012-06-13" version="1">MEVLISAVAGDLISRFISFLAQSYGSQTCEEDDRRRVEHLLLRIQIVVEEAEGRHITNRGMFLQLKALIEGVYLGYYMLDRLKLESLGEEIVDVAEVSHQSQSFAVSTFNTAKRLRFHAAGTKSTRKLKGVLECLETKITDMREFVMLLGNCPRLPHQPYSTYMYIDKCMFGRHIEKEQLINFLLYDSHDFTDLSILPIIGPHRIGKKTLVQHVCKDKRVHDHFSHMFFFRGEDLRKGEFLVNCKAASGKYLFVVDFSRDVDEAAWTKFRSYLKVPSTGTKIVLIGRAEQVANLGTARPIRLKCLSQEEYWYYFKVLSFGSMDPDEHPKLASLGMQLATELNGSFLAANILGEILRTNPNAPFWQKVLLSIKELVREHLMSFGTHPEDLLERNSPISFTKVALVGGQAQRYLVHDLREASSAQGELPRLTSQELLTGGDIPAEEMFEVLVWRSRIPPYCDYVVTYEKEKPSRRVSKKKNLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1INU0</accession>
    <name>I1INU0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100835260</name>
        <name evidence="4" type="ORF">BRADI_4g26510v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576355.1">
        <property type="nucleotide sequence ID" value="XM_003576307.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INU0"/>
    <dbReference type="EnsemblPlants" id="PNT64239">
        <property type="protein sequence ID" value="PNT64239"/>
        <property type="gene ID" value="BRADI_4g26510v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835260"/>
    <dbReference type="Gramene" id="PNT64239">
        <property type="protein sequence ID" value="PNT64239"/>
        <property type="gene ID" value="BRADI_4g26510v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835260"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_15_10_1"/>
    <dbReference type="OMA" id="EWNNASE"/>
    <dbReference type="OrthoDB" id="1882346at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000381">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001093">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At2g34400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF428">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="59"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="91"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="264"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="396"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT64239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="66630" checksum="0712EF5A669CA71D" modified="2012-06-13" version="1">MAPLLHARKLHASLLKSGGVSHQGHGGDTYRWNLLLRAYTRGGGLADARALLDLMPSPTLVSYNTLLSGYAASSGLLEAALHLLDAMPERDAWSWNVAISGLARAGRLTDALRRFLEMTRGPVQPDAFTYSIVSPCCCGGAGGGLESAWQVHARALKAGAFADACVGTGFVRLYSGLGLIGDARKVFEGMPERDLVAWNVLLDCGMRSGEAGSCLQDLVLMIGGRVQPDEFTFATVVNGLAERFAGLEAMQVHSVILKSGHLKDLFLCNSLLNVYGRCGYVDLAKKLFEAMPEKDVVSWTALISGLVASGHQADAFKTFCRMQGAATVPNSFTFGSVVSSCACVNDLGGGRQCHALVIKHGLESIPTVASSLLDMYSKCAEMDDATRMFDAMPRRDIVSWNAMICGLAQNGRSARALELYDEMLRLHQESITPNSVTCVGVLSACSHVGAVEKGCIYFTQMVNDFHIEPVPEHYSCLVDLFARAGLLDEAEGIISNSPFKHDAIILGTLLNGCRKYGNLDMAKRIAKRILVDDSDNASTIFLLSNMFIANEEWNDASELRKAVISSGTRKFVGNSWIDVGGNVHCFRAGSSPDPQLESIYDVLRQLQLTMVDADRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IRP0</accession>
    <name>I1IRP0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CFA20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100821555</name>
        <name evidence="2" type="ORF">BRADI_4g34860v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578406.1">
        <property type="nucleotide sequence ID" value="XM_003578358.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IRP0"/>
    <dbReference type="STRING" id="15368.I1IRP0"/>
    <dbReference type="EnsemblPlants" id="KQJ90959">
        <property type="protein sequence ID" value="KQJ90959"/>
        <property type="gene ID" value="BRADI_4g34860v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT64907">
        <property type="protein sequence ID" value="PNT64907"/>
        <property type="gene ID" value="BRADI_4g34860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821555"/>
    <dbReference type="Gramene" id="KQJ90959">
        <property type="protein sequence ID" value="KQJ90959"/>
        <property type="gene ID" value="BRADI_4g34860v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT64907">
        <property type="protein sequence ID" value="PNT64907"/>
        <property type="gene ID" value="BRADI_4g34860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821555"/>
    <dbReference type="eggNOG" id="KOG3213">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060610_1_1_1"/>
    <dbReference type="OMA" id="TTYISCP"/>
    <dbReference type="OrthoDB" id="7486196at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IRP0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040441">
        <property type="entry name" value="CFA20/CFAP20DC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12458">
        <property type="entry name" value="ORF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFA20" evidence="1">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ90959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="190" mass="22040" checksum="CB23D59FF6D971F8" modified="2012-06-13" version="1">MFKNTFQSGFLSILYSLGTKPLQIWDKEVVDGHIKRPQDDDIQSNVLEIIGANVQSTYITCPADPSATLGIKLPFLALIVKNLKKYFTFEVQVLDDKNVRRRFRASNFQSVTRVKPYICTMPLKLDDGWNNIQLNLSDLTKRAYGTNYVETLRVQVHANCRLRRVYFSDRLYSEEELPPEFKLYLPIQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IUH6</accession>
    <name>I1IUH6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">R3H-associated N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100828039</name>
        <name evidence="2" type="ORF">BRADI_4g42940v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578934.1">
        <property type="nucleotide sequence ID" value="XM_003578886.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IUH6"/>
    <dbReference type="FunCoup" id="I1IUH6">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IUH6"/>
    <dbReference type="EnsemblPlants" id="KQJ92331">
        <property type="protein sequence ID" value="KQJ92331"/>
        <property type="gene ID" value="BRADI_4g42940v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828039"/>
    <dbReference type="Gramene" id="KQJ92331">
        <property type="protein sequence ID" value="KQJ92331"/>
        <property type="gene ID" value="BRADI_4g42940v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828039"/>
    <dbReference type="eggNOG" id="ENOG502QQ6J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1108393_0_0_1"/>
    <dbReference type="OMA" id="PWGDEVP"/>
    <dbReference type="OrthoDB" id="75169at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02325">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025952">
        <property type="entry name" value="R3H-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039629">
        <property type="entry name" value="R3HDM4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32019">
        <property type="entry name" value="R3H DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32019:SF2">
        <property type="entry name" value="R3H DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13902">
        <property type="entry name" value="R3H-assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H-associated N-terminal" evidence="1">
        <location>
            <begin position="31"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ92331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="253" mass="29310" checksum="3DEDD51A8FC3326B" modified="2012-06-13" version="1">MASADLLRREEEFYESLFDSAKGDAAKTRGQMIERKIEALEDMATRVSNRRSRRWLNDRLLIELVPRLHVEEIKGLFAPPPWGDEQPLSAFCRTSAGEWDAFRSIDMDVEARFMQRMKQSSTKHKNGVNENELIVLNAWHRIDCQTREAMKRNFLPELLEIYEERVRTFIEDPSDKDVLVLNVQDPFQRLLLHGVCEFYNVASTTTTTIKDEKPWKTTTIKKRSGTGAPSMITLVAFMRAKKDQKPLQFVGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1IV27</accession>
    <name>I1IV27_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100842173</name>
        <name evidence="11" type="ORF">BRADI_4g44550v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238809.1">
        <property type="nucleotide sequence ID" value="XM_010240507.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758422.1">
        <property type="nucleotide sequence ID" value="XM_014902936.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024319231.1">
        <property type="nucleotide sequence ID" value="XM_024463463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IV27"/>
    <dbReference type="EnsemblPlants" id="KQJ92573">
        <property type="protein sequence ID" value="KQJ92573"/>
        <property type="gene ID" value="BRADI_4g44550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92574">
        <property type="protein sequence ID" value="KQJ92574"/>
        <property type="gene ID" value="BRADI_4g44550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92575">
        <property type="protein sequence ID" value="KQJ92575"/>
        <property type="gene ID" value="BRADI_4g44550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT65561">
        <property type="protein sequence ID" value="PNT65561"/>
        <property type="gene ID" value="BRADI_4g44550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT65562">
        <property type="protein sequence ID" value="PNT65562"/>
        <property type="gene ID" value="BRADI_4g44550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842173"/>
    <dbReference type="Gramene" id="KQJ92573">
        <property type="protein sequence ID" value="KQJ92573"/>
        <property type="gene ID" value="BRADI_4g44550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92574">
        <property type="protein sequence ID" value="KQJ92574"/>
        <property type="gene ID" value="BRADI_4g44550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92575">
        <property type="protein sequence ID" value="KQJ92575"/>
        <property type="gene ID" value="BRADI_4g44550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65561">
        <property type="protein sequence ID" value="PNT65561"/>
        <property type="gene ID" value="BRADI_4g44550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65562">
        <property type="protein sequence ID" value="PNT65562"/>
        <property type="gene ID" value="BRADI_4g44550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842173"/>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000837_25_0_1"/>
    <dbReference type="OMA" id="CAFINDS"/>
    <dbReference type="OrthoDB" id="693948at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IV27">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002758">
        <property type="term" value="P:innate immune response-activating signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1181">
        <property type="entry name" value="OS08G0170200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="192"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="432"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="553"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ92575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="907" mass="103492" checksum="F305EAA0A01BC5AE" modified="2012-06-13" version="1">MELATGAMSSLLPKLAELLQKEYNLQTGLKKDVQSLSRELERIEVALAKVAEVPLDQLDKQVRLWARNVKQLSYDMEDIVDSFMVRVEGSEPDADLKSSKRFRKKIANFFKKGKTRHQIADKIQDIKVRVKEVTDLRDRYKVDDVHANQAATNTIDPRMINLFKDQKELVGIEEPRKDLIKRLMEWGDVVSNSKLQLKIVSIFGFGGLGKTTLAKAVYDKLQEEFVCKAFISVGQKPNLKKVFMDILRQLDKNSYLNATMLDEVQLIDELRELLENKRYLIVIDDIWDKSSWNIMKCALIDSNCGSRIITTTRIFEVAEEADDVYKQKPLSSATSKELFYTRLSIGKCKITSGQPIEISEKILQKCGGVPLAIITIASLLASKPWEDWSEVYDSIGFGDGANIHVDNTRKILLYSYYDLPCYLRACLLHLSIYPEDHEIQKDTLIWKWVAEGFVHEKPGMGLFEIGERYFNELINRSLIQPVEEPYKSIIYACGVHDLVLDMICHLSKEENFITVHGSSKTEHQPSQSNVRRLAVQNIAMDEEPNSDNTEIRQVRSFNAIMCDVKRRSFLSGFQGLRILSMERCAFINDSSYHLENLGRLLQLRYLGLLGTPIRKLPKEIGNLRFLQVLDLRLTGIKELPESVGRLRRLKCLRLSRGFKGALGWIGNLVLIEELLLPYVSLEIVKELAKLTELREYGVCCEKFDDSILFNDMMKSLEHLQKLQAIQFECYTSWPRVDQANCVSYVLSHHLRRLELWVAFEKLPAWINSSSLPNLSHLSVGAGAVEAQDMEVLGRFPELVYLHLETLISIPDIVGGGAFPKLRYYHTNASARFLQGAMPSLECVVYYIRDECSGAKFERDIGSIGNLPCLDEVRLAFYRCASVTREKGEAALRQAVEVHPNNITVTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVI8</accession>
    <name>I1IVI8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF8003 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100837347</name>
        <name evidence="4" type="ORF">BRADI_5g00847v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239604.1">
        <property type="nucleotide sequence ID" value="XM_010241302.3"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IVI8"/>
    <dbReference type="EnsemblPlants" id="KQJ81459">
        <property type="protein sequence ID" value="KQJ81459"/>
        <property type="gene ID" value="BRADI_5g00847v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837347"/>
    <dbReference type="Gramene" id="KQJ81459">
        <property type="protein sequence ID" value="KQJ81459"/>
        <property type="gene ID" value="BRADI_5g00847v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837347"/>
    <dbReference type="eggNOG" id="ENOG502QVTJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005411_0_0_1"/>
    <dbReference type="OMA" id="QAGFVNW"/>
    <dbReference type="OrthoDB" id="122018at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IVI8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058316">
        <property type="entry name" value="DUF8003"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31513">
        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31513:SF2">
        <property type="entry name" value="MRAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26010">
        <property type="entry name" value="DUF8003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="878"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1292"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1320"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1364"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1405"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8003" evidence="3">
        <location>
            <begin position="798"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="693"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ81459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1447" mass="154619" checksum="6A7DC3A42D4E38A4" modified="2012-06-13" version="1">MVMRRVACWAVLFVAVGLIGGGGGGRLLAASPLWPEEEDPFRLSSADYDSGSEDAAVKVVEAFPCGAYSASTRSCEEMNGSGSFNTTCVITSSSSLDADVCVYGTGNVEIRPLVKIICPLKGCYIAFNVSGSITVGEHVEVIAGSVSLYATNVSLGRHTTINTTGMAGDPPSQTSGTPHSLEGAGGGHGGRGASCKVSNATNWGGDVYDWPELETPWSYGSKGGSLSADHQLGGDGGGRVMLRASEIMNVDGYVLAEGGVGSLRGGGGAGGSIIIQAVNLYGNGTISAAGGNGWGGGGGGRISLDFYSIQQDLEITVHGGQSFGCPQNAGAAGTIFECSLHSLKVSNGNFSTNTETPILGFSTKRLWSNVLVECNAKVLVPLLWSRVQVTGQIRVLSKGSICFGLSQRPVSEFELVAEELLMSDSVIKVYGAFRMYVKVLLMWDSKIQIDGGGDVILASMLEARNLVILKHGSVISSNAALGVYGQGLLNLSGPGDGIKARRLFLSLFYSIEVGPGSLLQAPLDEDVQSSLDALSACESKTCPTELITPPDDCHVNSSLSFTLQICRVEDITVGGIVRGSIIHIHRARTVTVTNDGAISASELGCKQGIGKGTFFKYGAAGGAGHGGQGGVGIYNGMRSVGGQQYGNAYLPCELGSGSGSAESADYSAGGGLIVIGSMKWPLSRLLIYGSVSSDGESNRGTTGNSNGTFKGGTGGGSGGTILFFLQRLLIEKNSSLSASGGNGGIHGGGGGGGGRIHFHWSNIATGDEYVQIASINGTVASSGGSGNEDGHIGEGGTVTGKKCPMGLYGIFCTECPVGTYKNVVGSNSSLCTACSLDSLPNRADFIYVRGGVTQPSCPYKCISAKYKMPNCYTPLEELIYTFGGPWFFAIILSAAIIILALILSAIRVKIGEGGVTYRATNAIQNDTYSSFPHLLSLAEVPGSIMRDETQSHVHRMYFMGPNTFREPWHLPYSPPDAIIPIVYEDAFNRFIDDINLVAAYEWWEGSVHSILSVLAYPCAWSWKQWRRRKKIHRLQEFVRSEYDHSCLRSCRSRALYKGLKVGSTPDLMVAYIDFFLGGDEKRLDVTFMIQKRFPMCLIFGGDGSYMSPYHLHCDSIQSNLLVQYVCTTTWNRLVDGLNAQLRTVKQGSIRSTLGPVVSWINSHANPQLELHGVRVELGWFQATASGYYQLGIVLAVNEHFYKNHDHHEHAPDSGDRSRKNFAVTLQCSRQASQGQPCGSNAVSRKRLTGGVNGGVINEGTLKSLEYKRDYLFPFSLLLQNCRPIGYAERLQLLICIVLVGDFSITLLMLVQYYWISVGAFLAVLLIPPLALLSPFLAGLNALFSRGPKRSSVTRIFTLWNTTSVVNIIVAIIYGAMYCGISSLTMSSVHASHTKGFKSREDNEWWILPLALFLVKSLQAGLVNWHVANLEIQDYSLFSLDPDRFWAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1IXF5</accession>
    <name>I1IXF5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-binding protein S1FA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100830477</name>
        <name evidence="11" type="ORF">BRADI_5g09140v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-binding protein that specifically recognizes a negative element (S1F) within the RPS1 promoter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the S1FA transcription factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239871.2">
        <property type="nucleotide sequence ID" value="XM_010241569.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IXF5"/>
    <dbReference type="STRING" id="15368.I1IXF5"/>
    <dbReference type="EnsemblPlants" id="KQJ82472">
        <property type="protein sequence ID" value="KQJ82472"/>
        <property type="gene ID" value="BRADI_5g09140v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ82473">
        <property type="protein sequence ID" value="KQJ82473"/>
        <property type="gene ID" value="BRADI_5g09140v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ82474">
        <property type="protein sequence ID" value="KQJ82474"/>
        <property type="gene ID" value="BRADI_5g09140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100830477"/>
    <dbReference type="Gramene" id="KQJ82472">
        <property type="protein sequence ID" value="KQJ82472"/>
        <property type="gene ID" value="BRADI_5g09140v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82473">
        <property type="protein sequence ID" value="KQJ82473"/>
        <property type="gene ID" value="BRADI_5g09140v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82474">
        <property type="protein sequence ID" value="KQJ82474"/>
        <property type="gene ID" value="BRADI_5g09140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100830477"/>
    <dbReference type="eggNOG" id="ENOG502S3X9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172811_0_0_1"/>
    <dbReference type="OMA" id="ANYVMYM"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006779">
        <property type="entry name" value="S1FA_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35298:SF11">
        <property type="entry name" value="DNA-BINDING PROTEIN S1FA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35298">
        <property type="entry name" value="DNA-BINDING PROTEIN S1FA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04689">
        <property type="entry name" value="S1FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007382"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ82472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="8836" checksum="7B8E9FABDBD735F8" modified="2012-06-13" version="1">MAEQFGDSANNVIIEEANKGLNPGMIVLLVVATFLLLFFVGNYALYLYAQKTLPPKKKKPVSKKKLKREKLKQGVSAPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I1IXV0</accession>
    <name>I1IXV0_BRADI</name>
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        <recommendedName>
            <fullName evidence="9">Protein EI24 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100834064</name>
        <name evidence="6" type="ORF">BRADI_5g10400v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003579725.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IXV0"/>
    <dbReference type="STRING" id="15368.I1IXV0"/>
    <dbReference type="EnsemblPlants" id="KQJ82671">
        <property type="protein sequence ID" value="KQJ82671"/>
        <property type="gene ID" value="BRADI_5g10400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834064"/>
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        <property type="gene ID" value="BRADI_5g10400v3"/>
    </dbReference>
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    <dbReference type="eggNOG" id="KOG3966">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="CysZ/EI24"/>
    </dbReference>
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        <property type="entry name" value="ETOPOSIDE-INDUCED PROTEIN 2.4 HOMOLOG"/>
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    </dbReference>
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        <property type="entry name" value="P53 INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07264">
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        <location>
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            <end position="72"/>
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        </location>
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        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ82671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="36219" checksum="AA8DC613BE3391CB" modified="2012-06-13" version="1">MESLASQAKPAAVLWLAGFLQAARLHRVFFFCARSRPLSIRIAQCFLLNGFIFLGSLLTLKSAVIPTILWILPEECDQLRGHLCDHTAAVATYSFLRSGLVEIFYVFWFYPLYIFSFIISTLWYGDIAKHALDVVKSKKLDASRAFDADTDKTSESADRPEGFDGLAIGVGEQVYSILLLTIFFAEVTVVGYIPYLGKAMNFLLLSLMYAYYCFEYKWNFFAVNLNHRLDFFESNWAFFAGFGSPCVLPIFFLSPLASYGVMAILYPLFVMTAAGTQAEQEIDELKPLHGGKLKRIPLFFLAKRLATQLLQLFPEAQKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZ05</accession>
    <name>I1IZ05_BRADI</name>
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        <recommendedName>
            <fullName evidence="4">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100827231</name>
        <name evidence="6" type="ORF">BRADI_5g13810v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580003.1">
        <property type="nucleotide sequence ID" value="XM_003579955.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZ05"/>
    <dbReference type="FunCoup" id="I1IZ05">
        <property type="interactions" value="2243"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IZ05"/>
    <dbReference type="EnsemblPlants" id="KQJ83228">
        <property type="protein sequence ID" value="KQJ83228"/>
        <property type="gene ID" value="BRADI_5g13810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827231"/>
    <dbReference type="Gramene" id="KQJ83228">
        <property type="protein sequence ID" value="KQJ83228"/>
        <property type="gene ID" value="BRADI_5g13810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827231"/>
    <dbReference type="eggNOG" id="KOG2085">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012437_4_1_1"/>
    <dbReference type="OMA" id="PHDPNTD"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051177">
        <property type="term" value="P:meiotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF51">
        <property type="entry name" value="SERINE_THREONINE PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ83228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="507" mass="57910" checksum="49FC38F7FA3F0AE2" modified="2012-06-13" version="1">MIKQILGKLPKKTVKAGDSRDAAMGNGYESSNSNSVVRSVEPAGHKRAGNGEYLAPAGSGPSPAMNGTVVYHSSEPLPAFKDVPASEKQNLFVKKVNLCCAVYDFTDPTKNLKEKEMKRQTLMELVDYVTSANGKFSEVIMLEIGKMVAINLFRSSRPNPRENKAIEGVDLEEDEPLMDPAWSHLQIVYEVFLRFVASQETDAKLAKRYIDHSFILRLLDLFDSEDLRERDYLKTILHRIYGKFMVHRPFIRKAINNIFYRFIFETERHNGVAELLEILGSIINGFALPLKEEHKLFLIRALIPLHKPKCVAMYHQQLSYSITQFVEKDCKLADTVIRGLLKYWPITNSSKEVMFLSELEEVLEATQLAEFQRCMVPLFRQIAGSMNSSHFQVAERALFLWNNDHIESLIKQNYKVILPIIFPALERNARGHWNQAVRSLTLNVRKIFSDHDPAFFGECMEKFNDDELKQEESSSKREALWKRLEEMVASKSNGNIPLGITNGKSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1J131</accession>
    <name>I1J131_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100831804</name>
        <name evidence="7" type="ORF">BRADI_5g19710v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the purine permeases (TC 2.A.7.14) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580442.1">
        <property type="nucleotide sequence ID" value="XM_003580394.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J131"/>
    <dbReference type="STRING" id="15368.I1J131"/>
    <dbReference type="EnsemblPlants" id="KQJ84267">
        <property type="protein sequence ID" value="KQJ84267"/>
        <property type="gene ID" value="BRADI_5g19710v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831804"/>
    <dbReference type="Gramene" id="KQJ84267">
        <property type="protein sequence ID" value="KQJ84267"/>
        <property type="gene ID" value="BRADI_5g19710v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831804"/>
    <dbReference type="eggNOG" id="ENOG502QRUH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043459_2_1_1"/>
    <dbReference type="OMA" id="GKIAMIY"/>
    <dbReference type="OrthoDB" id="1907510at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015211">
        <property type="term" value="F:purine nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030182">
        <property type="entry name" value="PUP_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376">
        <property type="entry name" value="OS09G0467300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376:SF36">
        <property type="entry name" value="PURINE PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16913">
        <property type="entry name" value="PUNUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ84267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="380" mass="40832" checksum="13F803EA0549B314" modified="2012-06-13" version="1">MADASSGNNNAAGSTSNAEVQIQIPAGPAKAEGSAAPSQKSGTKNWRWWLMVSVDAFFLVAGQTSATLLGRYYYHQGGSSKWVSAFVQTAGFPILYLALFCFPSKSPSSGAGRGDAPVAKIGVIYVVLGLIIAADDMMYASGLKYLPVSTYSLVCASQLAFNVVFSYVLNSQKLTGLIMNSVVLLTLSDALLGVNHEETEDVNGFSRGKYLMGFLLTLGASGTYSLILSLMQLTFENVIKKHTYSAVLNMQIYTALVATVATVFGLFASGEWRSLRGEMDAFESGQFSYFMTLVWTAVSWQVASVGVVGLVFEVSSLFSNVISTVALPVIPLFAVLIFHDKMDGIKIVSMLLALWGFVSYLMQHFIDDRKARKAAASGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1J3V4</accession>
    <name>I1J3V4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein CHROMATIN REMODELING 24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100839962</name>
        <name evidence="10" type="ORF">BRADI_5g27610v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579552.1">
        <property type="nucleotide sequence ID" value="XM_003579504.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J3V4"/>
    <dbReference type="STRING" id="15368.I1J3V4"/>
    <dbReference type="EnsemblPlants" id="KQJ85521">
        <property type="protein sequence ID" value="KQJ85521"/>
        <property type="gene ID" value="BRADI_5g27610v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839962"/>
    <dbReference type="Gramene" id="KQJ85521">
        <property type="protein sequence ID" value="KQJ85521"/>
        <property type="gene ID" value="BRADI_5g27610v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839962"/>
    <dbReference type="eggNOG" id="KOG0387">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_17_0_1"/>
    <dbReference type="OMA" id="HEEHDHQ"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J3V4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000055">
        <property type="entry name" value="Protein CHROMATIN REMODELING 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="301"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="652"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="117"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ85521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="999" mass="112433" checksum="277FF270A1C05DE4" modified="2012-06-13" version="1">MASPPSFDFFDDLDDEPATAHRQNPNPPLAAPTPNGLNDRLLRLAQSRPRPQNPNPDPLPPPPPPPEEVAGEPQDRKVKLAGRRRLCKISSSSPDADADDEGDNIRDILDDLTTRLDSLSVDRPKPRPRPQVAPLPCAIDNLDDDISLPHKGAKSTDEDRAPSPVDISSSDEVTVVQKVKKPQTLFSSAFTDRAQETNDKGKKTTAGAYGGGKFGKRASKPSSFLDYEDDRKEEEEEEAGGRAYEEKENRDTDADDIGWEKTEDFKMEPTGSGVMAKPYKLPGRIFKMLYPHQREGLRWLWVLHCRGTGGILGDDMGLGKTMQVSAFLAGLFHSRLIRRVLVVAPKTLLTHWMKELSVVGLKEKIRDYSGPSVNIRNSELQYAFRVGGILLTTYDIVRNNYKLMRGDFYNDADDEEEGRLWDYVILDEGHIIKNPKTQRAQSLLQIPCVHRIVISGTPIQNNLKEMWALFFFCCPEILGDKDEFKTRYELPILRGNDKSATNREKHIGSNVAKELRERIKPYFLRRMKSEVFLDTGSTDDKKLSKKNELIIWLKLTACQRQLYEAFLNSELVHSSMQGSPLAAITILKKICDHPLILTKRAAEGILEGMEGMDGLNNQEIGMVEKMAKNLADMAHDDDALQVDQEVSCKLSFIMSLLRKLLEEGHHVLIFSQTRKMLNLIQEAILLEGYKFLRIDGTTKISERERIVKDFQEGPGAQIFLLTTQVGGLGLTLTKAARVIVVDPAWNPSTDNQSVDRAYRIGQTKDVIVYRLMTSGTIEEKIYKLQVFKGALFRTATEQKEQTRYFSKRDIQELFSLPEQGFDVSLTQKQLQEEHEQQLVMDESLREHILFLEQQGIAGVSHHSLLFTKAAILPTLSENEALDNKPPTMPMMGRQYKASSSDYVANGAAHAFKPKEFTPRTYSPINTSSESPEEIKAKINRLSQTLANTTLVSRLPDRGDKLKKQIHDLDEKLTVIESSPESAASKGATEVICLDDRKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EL61</accession>
    <name>A0A0Q3EL61_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_4g17141v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EL61"/>
    <dbReference type="STRING" id="15368.A0A0Q3EL61"/>
    <dbReference type="EnsemblPlants" id="KQJ88334">
        <property type="protein sequence ID" value="KQJ88334"/>
        <property type="gene ID" value="BRADI_4g17141v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88334">
        <property type="protein sequence ID" value="KQJ88334"/>
        <property type="gene ID" value="BRADI_4g17141v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EL61"/>
    <dbReference type="OrthoDB" id="762143at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF36">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="184"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="422"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="11">
        <location>
            <begin position="570"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ88334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1006" mass="114554" checksum="C04370509E63A651" modified="2016-01-20" version="1" fragment="single">MAMVLDAFASYVSDLLMQVVGEEVRTVLAITGDINKMSDKLEKLKKFLADADRRNITDESVQGWVTELKRAMYEATDILDLCQLKAMERGASTANVGCCKPLLFCMPNPFHAHEIGTRIKALNQRLDNIEKQSASFGFILGSYEDRGRRVQAENSSHTRESTGQLDRSVVVGEKIEEDTTALVAKILQSRNEVNNNIMVVAIVGVGGIGKTTLAQKVFNDDSIEGEFDKKIWLSINQNFDKTELPRTAITLAGGEHHGEKVLAVLQPILTTTLTGKKFLLVMDDLWSHGAWEGVLKIPLVNAAALGSRVLITTKHEGSTRYRLTTLGRCSRNSERDEDHINTLKDIGLKIIEKCGCLPLAVKVMGGLLRQREMLRRDWEQVLDDSKWSVSKMPQELNYALYLSYEDMPPYVKQCFLHYYLLPKSNRFDVLHVIGMWISEGFIHGNSSDLEESGRSYYKELISRNLIELDKSDFDQKYCSMHDVVRSFGQYMARDEALVADDGEIDILAKLKSQKFLRLSIETNQFQSGELDWKSLQEQQSVRTLITTIQIKMKPGDSLLTFSSLRTLICSHLRYFKLLNASISALPGNIGNMKLLQYLDIRGCINLVYLPDSIVKLGQLRYLNLPNLCMIPREFRCLTNMRILGGFPALMDGDWCSLDELGPLSQLRFLRLAQLENVSSAANAKLGEKKHLIHLLLYCTRRREHDGLDEQRQIEKVFNELCPSPSVENFNIYGYFGQQLPSWMMSTSMVPLKNLKFLLISDLAYCTHLPNGLCQLPYLQFLQVSHAPSIRHVGTEFLHPSQETAAAFPRLQKLELFGMVEWEEWEWEEQVQALSRLEELFLDNCKLWRVPPSLASQARSLRKLSINCVRQLSFIENFPFVVELTVLGCPDLERITYLPKLHNLTITDCPKLMVLKGVHALQRLVLDDEKMETVHEYTKDVKPRYLELKCSITLLTSIAEGQCGPEFAKFNHIDHVKAYANGRDWYMLYTRNPCSFQTNINLLFLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ELR3</accession>
    <name>A0A0Q3ELR3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIB1-4 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g18950v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ELR3"/>
    <dbReference type="STRING" id="15368.A0A0Q3ELR3"/>
    <dbReference type="EnsemblPlants" id="KQJ88503">
        <property type="protein sequence ID" value="KQJ88503"/>
        <property type="gene ID" value="BRADI_4g18950v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88503">
        <property type="protein sequence ID" value="KQJ88503"/>
        <property type="gene ID" value="BRADI_4g18950v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3ELR3"/>
    <dbReference type="OrthoDB" id="694239at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005174">
        <property type="entry name" value="KIB1-4_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45560:SF5">
        <property type="entry name" value="DUF295 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45560">
        <property type="entry name" value="OS04G0163150 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03478">
        <property type="entry name" value="Beta-prop_KIB1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIB1-4 beta-propeller" evidence="1">
        <location>
            <begin position="105"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ88503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="225" mass="25729" checksum="F23F4FE4080DAA09" modified="2016-01-20" version="1">MEAFMRIRFSGQHQASWGPRWEPQPRSFSLGMKQMRDPGSYAAMLILHIDFGATAFATAEDPMWRVAYSPGGIEDAIHVKGQFYSVSCFGDMEVWKYGAEASKMRSMMCAFRVYILDVDGRGAGRQWKESKNIVKSALFVGLNNSLCVSTRQHLDIKSNRIYFTDDELEHAVGRSNVWNQCLYNSRGYENRCIGVYNIKKHILKKVKSPMEEASFWPPAVWFSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EM40</accession>
    <name>A0A0Q3EM40_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_4g10686v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EM40"/>
    <dbReference type="EnsemblPlants" id="KQJ87388">
        <property type="protein sequence ID" value="KQJ87388"/>
        <property type="gene ID" value="BRADI_4g10686v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87388">
        <property type="protein sequence ID" value="KQJ87388"/>
        <property type="gene ID" value="BRADI_4g10686v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EM40"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ87388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="112" mass="12708" checksum="527D4DC1288B45E4" modified="2016-01-20" version="1">MKLYTSFKKGGKWQRAQDGAGWLRSQQDHRHHRLLWVHVGESNLNAETLMLTCKSTRIVSISSMLLDHMLLPSEGSEACFFEIAAIVLSMQNTAKTGKQEILRRMLVDAHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FA07</accession>
    <name>A0A0Q3FA07_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GAGA-binding transcriptional activator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100846523</name>
        <name evidence="9" type="ORF">BRADI_3g21390v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transcriptional regulator that specifically binds to GA-rich elements (GAGA-repeats) present in regulatory sequences of genes involved in developmental processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the BBR/BPC family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FA07"/>
    <dbReference type="STRING" id="15368.A0A0Q3FA07"/>
    <dbReference type="EnsemblPlants" id="KQJ96174">
        <property type="protein sequence ID" value="KQJ96174"/>
        <property type="gene ID" value="BRADI_3g21390v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96174">
        <property type="protein sequence ID" value="KQJ96174"/>
        <property type="gene ID" value="BRADI_3g21390v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1903765at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FA07">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009723">
        <property type="term" value="P:response to ethylene"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010409">
        <property type="entry name" value="GAGA-bd_tscrpt_act"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31421">
        <property type="entry name" value="PROTEIN BASIC PENTACYSTEINE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31421:SF22">
        <property type="entry name" value="PROTEIN BASIC PENTACYSTEINE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06217">
        <property type="entry name" value="GAGA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01226">
        <property type="entry name" value="GAGA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="176"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ96174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="376" mass="41442" checksum="A8C1EEF2D1BFC812" modified="2016-01-20" version="1">MDDDGSLGMRNWGFFDPPTRNNLGLQLMSSMPADRRDTKQLLSTGPYVHQHHHVHPHAPQHTHHHPHPHPHPRDGCGGVSSGAPGDSTYIPVNFVRNEAWLHQAHHPREPKILHAITAGHSGHVVHAAHHEQAGYGIIPGTHGMHTLQMMQKAEPQPPPPPKDECISPPLVEENAGVVNELPPPKKKQQRRQPKSPKPKKPKKANAPCEDGVPKPAPRKRGPKKHVGMVINGIDLDLSRIPTPVCTCTGAQQQCYRWGAGGWQSACCTTTISTYPLPMNTKRRGARIAGRKMSHGAFKKVLEKLAGEGYNLNNPIDLKTFWAKHGTNKFVTIRPSLIIMKGPNTACVFNLNVFCFFVVLPKLTVNLAANNEDTLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FTT3</accession>
    <name>A0A0Q3FTT3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g02866v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FTT3"/>
    <dbReference type="EnsemblPlants" id="KQK02655">
        <property type="protein sequence ID" value="KQK02655"/>
        <property type="gene ID" value="BRADI_2g02866v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK02655">
        <property type="protein sequence ID" value="KQK02655"/>
        <property type="gene ID" value="BRADI_2g02866v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FTT3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK02655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="280" mass="31607" checksum="C0B463E55F809823" modified="2016-01-20" version="1">MINRCRARTRTKVRNTTSSVIVPPDASLIPPNITCSSPTGALPSHRIVSVQIGAECTFIGSDCSTSQSSAVNFSLIQHSILEVDFLPMVMAAKFQITFFFLSQVMTRGLLLHVSVEIVLHCCIILAVSDNQITCLKTKLTSHGPRLLGVLVCFRFSFKRRKKMQVRILFILYTQYTTSSLPCLFFLTENCRGNPYYSLPCLETRLLKDLRIKQAGNRKVVVYISKKMQLASYYEGLTYKVLCVIVYMSNCVLSSQDTTPPEVNCMYMWSSSLSTWYRGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FVP4</accession>
    <name>A0A0Q3FVP4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g08174v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03488.2"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK03488"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="242" mass="24992" checksum="5F8B8B3DB4ACE190" modified="2018-03-28" version="2">MSGTAQASEKHILSCRRCFCPLDSVSGTCRTRERETRDTRELRSCSSQVWIEKTLASAHRFSASDCFPPSPPPDPRASSHGGRAGPRTEPRGTAGSGSLHLAKRKQGLWRRGCSATPRCRSTSCKLRDGLAAVPATSDVPAGAAAVPTWLQLPRAPSSVAIPRSLPNVCAMDGWAGSFSSDPTCTCSTSSACSSGPTSAATTTTAGAAEESGAETATAATTTPATAEGENGSDQSFLGERTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="14">Bd21</strain>
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    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <property type="gene ID" value="BRADI_2g25984v3"/>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK08016"/>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87674.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H2M7"/>
    <dbReference type="EnsemblPlants" id="KQJ87674">
        <property type="protein sequence ID" value="KQJ87674"/>
        <property type="gene ID" value="BRADI_4g12881v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87674">
        <property type="protein sequence ID" value="KQJ87674"/>
        <property type="gene ID" value="BRADI_4g12881v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H2M7"/>
    <dbReference type="OrthoDB" id="657261at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF512">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="3">
        <location>
            <begin position="8"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ87674.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="281" mass="31228" checksum="D540E65E70CEAE3D" modified="2018-03-28" version="2" fragment="single">TIVAPTVSGSHIVRIDGYSGTKGLGNGKFLKSEAFDVGGHSWYNADWIAIYLRLHHNNSSEVIAQYKISLLDKDGQPVPSYSRSSSKSRTFTSEEPSLFDFAQFIKRKDLEESPYLKDDVFSIRFDVTVQKDIFTGPILPSLLTAKVPVPPSDMAQHLAQLLLAGEAADITFDAELLGPMKEKTATRIQIHDMEAKVFKALLHFIYTDMVPQMDEAYTIKHRTVATTLTLAEQHGCGKLKTACFKLLASPGNLKEIMKSDCFEHLKSSCPYILEELIANLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H3C8</accession>
    <name>A0A0Q3H3C8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g14279v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H3C8"/>
    <dbReference type="EnsemblPlants" id="KQJ87930">
        <property type="protein sequence ID" value="KQJ87930"/>
        <property type="gene ID" value="BRADI_4g14279v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ87930">
        <property type="protein sequence ID" value="KQJ87930"/>
        <property type="gene ID" value="BRADI_4g14279v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H3C8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ87930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ87930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="83" mass="9201" checksum="A54EEEA22136F84B" modified="2016-01-20" version="1">MSIKRKSLRWWCLGDERVSPSCRPSRGSRGTSTRGDAGGRLRPAPLLATGWPSEMKTKWQEGASATRTSAFDRPGQACFFAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HXF3</accession>
    <name>A0A0Q3HXF3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_3g01817v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ92955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HXF3"/>
    <dbReference type="FunCoup" id="A0A0Q3HXF3">
        <property type="interactions" value="437"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ92955">
        <property type="protein sequence ID" value="KQJ92955"/>
        <property type="gene ID" value="BRADI_3g01817v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ92955">
        <property type="protein sequence ID" value="KQJ92955"/>
        <property type="gene ID" value="BRADI_3g01817v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HXF3"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HXF3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051114">
        <property type="entry name" value="Mito_RNA_Proc_CCM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934:SF6">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN PET309, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="214"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="249"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="284"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ92955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="39549" checksum="4FCC5993F685BCD1" modified="2016-01-20" version="1">MGKVQHAMRAARGLAPKPSTPSIPAVPSGTRTTQHPKPHGNKKPRGAAAPKASGTAPAEAPAPVTADKRRPITSPAELSSAIRATVDKDVDAAASLALKAAPTIPIPVQSLALLLRRLAAHRSVSAARDLLAGLSFGPSSPAPRPALLALADACCRRGDPREIGQQLLPVLADHGVRADAHVYNALMKAHCAASDPEGVLGVLRRMKGDGVDPDLVTYNTLVFGLARAGMVAKARTYVDAMAAEGHFPDVITYTSLMNGMCVKGDALGALKLLEEMEAKGCEPNARTYNTLLMGLCKNRKLDKAVEVYRSMVGAAMKLETPAYASFIRALCRAGRVPDAYEVFDYGIESKSLAQVTAYSELESSLKWLRRMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I7W9</accession>
    <name>A0A0Q3I7W9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g07206v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3I7W9"/>
    <dbReference type="FunCoup" id="A0A0Q3I7W9">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ82184">
        <property type="protein sequence ID" value="KQJ82184"/>
        <property type="gene ID" value="BRADI_5g07206v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82184">
        <property type="protein sequence ID" value="KQJ82184"/>
        <property type="gene ID" value="BRADI_5g07206v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3I7W9"/>
    <dbReference type="OrthoDB" id="674857at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994:SF25">
        <property type="entry name" value="OS02G0619200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994">
        <property type="entry name" value="OS04G0515000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ82184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="21441" checksum="670B38863ECDDDAD" modified="2016-01-20" version="1">MYKEDCCFVVAIVTVLCLLAGLGTFIGMDEYRKPRYYAAIDSVSGLDLGRPSSLLDPVFNVTLRVASLGFRHRECIAPGTVVEVSYHGSPLASGAAGQLCARPRGGAGAREESLVAWGRGVRVPGFALDGLAADARQGVQAFDVTLQMPPTDINHHMGKVVTCSARRAGDAGALEAPCDSSDMGQYWLPSPTNLHGGANSTTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IBF0</accession>
    <name>A0A0Q3IBF0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carboxypeptidase</fullName>
            <ecNumber evidence="2">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_3g32259v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IBF0"/>
    <dbReference type="STRING" id="15368.A0A0Q3IBF0"/>
    <dbReference type="EnsemblPlants" id="KQJ97619">
        <property type="protein sequence ID" value="KQJ97619"/>
        <property type="gene ID" value="BRADI_3g32259v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97619">
        <property type="protein sequence ID" value="KQJ97619"/>
        <property type="gene ID" value="BRADI_3g32259v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3IBF0"/>
    <dbReference type="OrthoDB" id="693328at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IBF0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF223">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ97619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="166" mass="18451" checksum="1C55AE41A69E2D4B" modified="2016-01-20" version="1">MAVQELDLVVSLPEAPSCSSAFKQYSGYVTTDEHLGKTLFDWLFEAADKPDEKPLVLWLNGGPGCSTVGFGQAQELGPFRVKKDVPELEFNQYAWNKAANLLFLDSPAGVGFSYTNTSFEQDPPGDNSTAHGSYTFLVKWFQRFPQHKMKEFYIAGESYAGPPTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IZ93</accession>
    <name>A0A0Q3IZ93_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC104583140</name>
        <name evidence="7" type="ORF">BRADI_2g22045v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK05708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010233188.1">
        <property type="nucleotide sequence ID" value="XM_010234886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3IZ93"/>
    <dbReference type="STRING" id="15368.A0A0Q3IZ93"/>
    <dbReference type="EnsemblPlants" id="KQK05708">
        <property type="protein sequence ID" value="KQK05708"/>
        <property type="gene ID" value="BRADI_2g22045v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583140"/>
    <dbReference type="Gramene" id="KQK05708">
        <property type="protein sequence ID" value="KQK05708"/>
        <property type="gene ID" value="BRADI_2g22045v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583140"/>
    <dbReference type="OrthoDB" id="1910624at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IZ93">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF2">
        <property type="entry name" value="OS05G0199100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="6">
        <location>
            <begin position="79"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK05708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK05708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="206" mass="21840" checksum="A75F45EAE8501CB7" modified="2016-01-20" version="1">MAAPGPRQHRRRRALVRASFAALAVLLLAAAAAILVLTLFRPRPPTTELLSATATGAMPRVTLLPTISIQLNVTFLLVVRVRNPNAASFSFRPGDQPGGSTARLYYRGAPVGEAAVPAGDVPSRGEATLRMDMTVQGEKVAAAAGLGALVGDVLAGEMEFEARTDVKGRVTFLGFVKRSAEARSVCRIVVGVVDVKVRRQECHTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J209</accession>
    <name>A0A0Q3J209_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Drought induced 19 protein type zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100836146</name>
        <name evidence="4" type="ORF">BRADI_2g28040v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Di19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231504.1">
        <property type="nucleotide sequence ID" value="XM_010233202.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J209"/>
    <dbReference type="FunCoup" id="A0A0Q3J209">
        <property type="interactions" value="930"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3J209"/>
    <dbReference type="EnsemblPlants" id="KQK06724">
        <property type="protein sequence ID" value="KQK06724"/>
        <property type="gene ID" value="BRADI_2g28040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836146"/>
    <dbReference type="Gramene" id="KQK06724">
        <property type="protein sequence ID" value="KQK06724"/>
        <property type="gene ID" value="BRADI_2g28040v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3J209">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033347">
        <property type="entry name" value="Di19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027935">
        <property type="entry name" value="Di19_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31875">
        <property type="entry name" value="PROTEIN DEHYDRATION-INDUCED 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31875:SF26">
        <property type="entry name" value="PROTEIN DEHYDRATION-INDUCED 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14571">
        <property type="entry name" value="Di19_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Di19 zinc-binding" evidence="2">
        <location>
            <begin position="48"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Di19 C-terminal" evidence="3">
        <location>
            <begin position="122"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK06724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="26962" checksum="B0E25133887F91CA" modified="2016-01-20" version="1">MDAGDAWGRSSSSSSSAARRLQSRYDLYMGFDEADAGGEEVEARGAEAYNCPFCGEDFDFVGLCCHIDDEHAVEAKSGVCPICATRVGMDLIGHLTMQHGSYFKMQRRRRVRKVSSGSHSLLSLLRKDLRDGNLESFLGGSSYVSNPPAAAPDPFLSSLICSLPVAEPSKDFHSDSSDKTFLLNKFPDEKTVERLLNPFSDVKMCFALFRAETSLSEKDQKERVQRSEFARGLVLSTIFDEDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JH17</accession>
    <name>A0A0Q3JH17_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g61485v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK11660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JH17"/>
    <dbReference type="EnsemblPlants" id="KQK11660">
        <property type="protein sequence ID" value="KQK11660"/>
        <property type="gene ID" value="BRADI_2g61485v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK11660">
        <property type="protein sequence ID" value="KQK11660"/>
        <property type="gene ID" value="BRADI_2g61485v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JH17"/>
    <dbReference type="OrthoDB" id="595071at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK11660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="83" mass="8934" checksum="35BB90C1E84B45B8" modified="2016-01-20" version="1">MAATMDCAIQVVLRVVMRGSICRLHEAVAMAVELGTALLMAVRFSGTAFRRNPAAVPGSSTRYYYNAPVSAASMVGMSRLDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JQ43</accession>
    <name>A0A0Q3JQ43_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC104582258</name>
        <name evidence="2" type="ORF">BRADI_1g50890v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229974.1">
        <property type="nucleotide sequence ID" value="XM_010231672.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312807.1">
        <property type="nucleotide sequence ID" value="XM_024457039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JQ43"/>
    <dbReference type="STRING" id="15368.A0A0Q3JQ43"/>
    <dbReference type="EnsemblPlants" id="KQK19859">
        <property type="protein sequence ID" value="KQK19859"/>
        <property type="gene ID" value="BRADI_1g50890v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT76636">
        <property type="protein sequence ID" value="PNT76636"/>
        <property type="gene ID" value="BRADI_1g50890v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT76637">
        <property type="protein sequence ID" value="PNT76637"/>
        <property type="gene ID" value="BRADI_1g50890v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT76638">
        <property type="protein sequence ID" value="PNT76638"/>
        <property type="gene ID" value="BRADI_1g50890v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104582258"/>
    <dbReference type="Gramene" id="KQK19859">
        <property type="protein sequence ID" value="KQK19859"/>
        <property type="gene ID" value="BRADI_1g50890v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76636">
        <property type="protein sequence ID" value="PNT76636"/>
        <property type="gene ID" value="BRADI_1g50890v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76637">
        <property type="protein sequence ID" value="PNT76637"/>
        <property type="gene ID" value="BRADI_1g50890v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76638">
        <property type="protein sequence ID" value="PNT76638"/>
        <property type="gene ID" value="BRADI_1g50890v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104582258"/>
    <dbReference type="OrthoDB" id="1917939at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JQ43">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010705">
        <property type="term" value="P:meiotic DNA double-strand break processing involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48221">
        <property type="entry name" value="ACYL-COA SYNTHETASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48221:SF2">
        <property type="entry name" value="ACYL-COA SYNTHETASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK19859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="707" mass="78078" checksum="8711DF33E31CDFC9" modified="2016-01-20" version="1">MAPPLDGLTKLLAEVASRLSRPPGVIGAGPSSSSSSSGDSLSASISSLAAALNPRPAAAATGTRVLDAALSLMCFDPLEVDRARVECLVRTITSALSASVSCRVVRTDDCGGEMLCVGSSVSPGDCRELVSLCASLVEKLGDRDGHSYDLLHTVVKTTLLSPRYQCLFPLPYYRENGEKKYEMATVAVDLTRHPSYQVIPSDGSIPLRVLLWHLDPSILKHDLSAMLHEAITRPFLCLRKELHDRIAWRVIIICLVCFPPAFLEMTSLFHSWFLATGMCSVLGLCTAMVSSVLDVLLEPMGWGISMELGQKFPFTHAYFPSEHSDLLSILTGPISCGRFLDLVSYIEAMVYSGKTRAGNSKSSKGTHFGNKQLSKGLLKFKYSSAWSTITNFPIWFSFAAALLFHREGFQGYLSEILSEEKVSESISDISLARRAAFYLSWVLCPSNEDECQMLANNMVELSNSWARNIKSRPSYAYHTSTVNHRRRLRIPTVGDTEKLHMSTNPVSSLIQEFDNRCVNFCNQTAVSQVQDELSGFPPSCISFLHLQIPLGVLLVSSSCVKDHDHDVLLHYASSGLILEADKVQTKTKDHVGNHGFSASRRGFTERWALSGACVIFSWFDVIDDMSAVIFECEDTCQHFVSELRTKTSPYLINCVNLVLNEAGQDKDSVIDLHDRLLDWNNKLKSFDGCEAFKDVILQLKKKVLLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JX19</accession>
    <name>A0A0Q3JX19_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BRADI_2g04930v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JX19"/>
    <dbReference type="EnsemblPlants" id="KQK03001">
        <property type="protein sequence ID" value="KQK03001"/>
        <property type="gene ID" value="BRADI_2g04930v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03001">
        <property type="protein sequence ID" value="KQK03001"/>
        <property type="gene ID" value="BRADI_2g04930v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JX19"/>
    <dbReference type="OrthoDB" id="775830at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JX19">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051708">
        <property type="entry name" value="Plant_Aspart_Prot_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967:SF128">
        <property type="entry name" value="ASPARTIC PROTEINASE CDR1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47967">
        <property type="entry name" value="OS07G0603500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033725072" description="Peptidase A1 domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="9">
        <location>
            <begin position="73"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK03001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="457" mass="46839" checksum="E8C84B66B0483232" modified="2016-01-20" version="1">MEATLLLAGVLLLAGVCGAAYPGDGGFSVELIHRDSIKSPFHDPKLTRHDRARAAALLASDVSSDLFYGDFEYLAAVNVGTPPVRFLAVADTGSDLVWLKCNTTQNNNGIVSSDSGNNSNSSPPPPPPEAVVYFNPFDSSSYSRVGCDGPSCLALATNASCNGDSHACDFRYSYRDGASATGLLAADTFTFGGNINNDTTSTASIDFGCATGTAGREFQADGMVGLGAGPLSLASQLGRKFSFCLTAYDIDDASSILNFGARAVVSDPGAATTPLIASSSNAAAYYAISIDSLKVAGQPVPGTTSVSKVIVDTGTVLTFLDRAALLAPLTESLARVMDGAGLPRAPPPDETLELCYDVSRVKDVDGVIPDVTLVLGGGGGGEVRLTGEGTFVLVKEGVLCLAVVTTSPELQPLSVLGNVALQDLHVGIDLDARTATFATANCDSSSRATVNSVAADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3L022</accession>
    <name>A0A0Q3L022_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g30100v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3L022"/>
    <dbReference type="EnsemblPlants" id="KQK16705">
        <property type="protein sequence ID" value="KQK16705"/>
        <property type="gene ID" value="BRADI_1g30100v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16705">
        <property type="protein sequence ID" value="KQK16705"/>
        <property type="gene ID" value="BRADI_1g30100v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3L022"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK16705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="167" mass="19225" checksum="1609591428D03B00" modified="2016-01-20" version="1">MFQKKNNAHTTHARKTPVPLQVRLINPMEIAPSSAPPSAAAAGSRKLPPVRCFVPYEVEEDPVFDPDSPRSPSEQRAEDSGGGGDQEKMHLECYNYKTASTWVRQSDKYAEMHYNGDPGNEIKYELVDRAPREQRWHHDRQRPHARPRQLHRQARRRRPRRTRGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3L6H0</accession>
    <name>A0A0Q3L6H0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100828348</name>
        <name evidence="4" type="ORF">BRADI_1g44767v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560868.1">
        <property type="nucleotide sequence ID" value="XM_003560820.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3L6H0"/>
    <dbReference type="STRING" id="15368.A0A0Q3L6H0"/>
    <dbReference type="EnsemblPlants" id="KQK18801">
        <property type="protein sequence ID" value="KQK18801"/>
        <property type="gene ID" value="BRADI_1g44767v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828348"/>
    <dbReference type="Gramene" id="KQK18801">
        <property type="protein sequence ID" value="KQK18801"/>
        <property type="gene ID" value="BRADI_1g44767v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828348"/>
    <dbReference type="OrthoDB" id="341587at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3L6H0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001374">
        <property type="entry name" value="RNI-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK18801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="614" mass="66014" checksum="FCD1D88493B335C2" modified="2016-01-20" version="1">MESSLLLSSSRPLKPSLLAPSSRAYRRHACFGSSPLSRCLLPSPPPRARRWTMAPRAVSEVFDGIHSQHNPPGVGRGGARRKAYREVQGEPAAPPLAVPARGVTGYIVPAGALLALSFVVWRVVLNFLPGKKKDRTSAEPTSSGIMWSFAAGSNLSTSTSFKAEKESKKNLNRFYKELRTLKNVNMAGRQFGDEGLFFLAESLAYNKSAEEVDFSGNAITAVGIEAFDGILQINTALKTLNLSGNAIGDEGAKCLSGILVENVGIQKLLLNSTNIGDEGAKAISDMLKKNKTIRIVQLSNNVIEYSGFASIAEALLENNALRSLYLNGNYGGPLGASSLAKGVVGNKSLRELHLHGNGFVNEGLRVLMSALSSHKGKITVLDIGNNNITSEGSLYVADFIKRTKSLRWLSLYMNDVGDEGAEKVADALKQNQTISTMDFGGNNIHSRGVTAIAETLKENAVLTTLELSYNPIGPEGVKALCDVLKFDGKLQTLKLGWCQIGVSGAEFIADCLKCNTTLSTLDLRANGLGDDGAICLARSLKTINESLKSLDLGFNEIRDDGAFALAQALKANEDLAITSLNLANNFFGKFGQVALTEARDHVYEMSEKEIDIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LDX5</accession>
    <name>A0A0Q3LDX5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100827114</name>
        <name evidence="7" type="ORF">BRADI_4g33878v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578337.2">
        <property type="nucleotide sequence ID" value="XM_003578289.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LDX5"/>
    <dbReference type="SMR" id="A0A0Q3LDX5"/>
    <dbReference type="FunCoup" id="A0A0Q3LDX5">
        <property type="interactions" value="1505"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3LDX5"/>
    <dbReference type="EnsemblPlants" id="KQJ90781">
        <property type="protein sequence ID" value="KQJ90781"/>
        <property type="gene ID" value="BRADI_4g33878v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827114"/>
    <dbReference type="Gramene" id="KQJ90781">
        <property type="protein sequence ID" value="KQJ90781"/>
        <property type="gene ID" value="BRADI_4g33878v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827114"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LDX5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11733">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000020">
        <property type="entry name" value="Chaperone protein HscA homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000001">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="640"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="648"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ90781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="680" mass="72478" checksum="87D8E139F507127A" modified="2016-01-20" version="1">MAIGSLVASRLARSGHALATAAISQAPRSRHTTSPLLSRLGAVARAFSSKPAAADVIGIDLGTTNSCVSVMDGKTPRVIENAEGARTTPSIVAKNQNGDLLIGITASRQAVTNAQNTVRGSKRLIGRTFDDPQTQKEMKMVPYKIVRGPNGDAWVEMGGQKYSPSQIGAFVLTKMKETAESFLGKTVSKAVITVPAYFNDAQRQATKDAGRIAGLEVMRIINEPTAAALSYGMNNKEGLIAVFDLGGGTFDVSILEISNGVFEVKATNGDTFLGGEDFDAALLDYLISEYQKSDNIDLSKDKAVLQRLREAAEKAKVELSSTTQTEINLPFITADASGAKHFNITLTRSKFESLVGNLIERTRIPCVNCLKDAGISAKEIDEVLLVGGMTRVPKVQDIVSQIFNKAPSKGVNPDEAVAMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLINRNTTIPTKKSQTFSTAADNQTQVGIKVLQGEREMATDNKLLGEFQLEGIPPAPRGMPQIEVTFDIDANGIVRVSAKDKSTGKEQDITIKSSGGLSERDIENMVKEAELHSQRDQEKKSLIDLKNSADTTIYSIEKSVSEYKDKVPSEITTEVESAVSDLRAAMAGDDLDNIKQKLEAANKAVSKIGQHMQGGGGDAGGSSGGSGGDQTPEAEYQDPKEAKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LEF9</accession>
    <name>A0A0Q3LEF9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_4g35180v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LEF9"/>
    <dbReference type="FunCoup" id="A0A0Q3LEF9">
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    <dbReference type="STRING" id="15368.A0A0Q3LEF9"/>
    <dbReference type="EnsemblPlants" id="KQJ91029">
        <property type="protein sequence ID" value="KQJ91029"/>
        <property type="gene ID" value="BRADI_4g35180v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ91029">
        <property type="protein sequence ID" value="KQJ91029"/>
        <property type="gene ID" value="BRADI_4g35180v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="681562at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00360">
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    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="60"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ91029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="147" mass="16270" checksum="B702752129CB708A" modified="2016-01-20" version="1" fragment="single">CGVCGAGRSHDSAPFCPYNYINGQYGLGTCREVCRPGRHRSLHELGSYSGSGGNIHPLGRVVRVNNVPLTPNEIPPDQRELRGLFGRFGPLVDCHLTRTSVQDLVGFGFVAFESCEHAREAVDKLNGHHFGDRRLRVDWAYPRAARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3NTT0</accession>
    <name>A0A0Q3NTT0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g57260v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3NTT0"/>
    <dbReference type="EnsemblPlants" id="KQK20878">
        <property type="protein sequence ID" value="KQK20878"/>
        <property type="gene ID" value="BRADI_1g57260v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK20878">
        <property type="protein sequence ID" value="KQK20878"/>
        <property type="gene ID" value="BRADI_1g57260v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NTT0"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3NTT0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000022">
        <property type="entry name" value="F-box protein FBW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF70">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF12937">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK20878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="299" mass="34761" checksum="858F688219F3729D" modified="2016-01-20" version="1">MGEECNEYRCWEELLPDALGLIFRNLSLQEVLTVVPRVCKSWNRVVSGPYCWQEIDIDEWCQRNWWKSEKLTRMVHMLIARSGGSFSRFSVSALPNDTLFASLQTSNARSLKTFELPRSDISDCIVEDVAQRLSNVTFLDVSSCTKIGARALEAFGKNCRSLERLWHVMHPMDVDGKDSQHDEARAIAYNMRKLRHLEIGYMLIATEDVVEIASQCHDLKFLDVRGCWYVDNKLLEERYPWLKVQGPLVDGFYEDRFWEEYSDDSDDDSIYSSELIDDDYYVIGSDEEEAIWDDGQDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3PP64</accession>
    <name>A0A0Q3PP64_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100843091</name>
        <name evidence="5" type="ORF">BRADI_4g36621v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576781.1">
        <property type="nucleotide sequence ID" value="XM_003576733.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3PP64"/>
    <dbReference type="STRING" id="15368.A0A0Q3PP64"/>
    <dbReference type="EnsemblPlants" id="KQJ91257">
        <property type="protein sequence ID" value="KQJ91257"/>
        <property type="gene ID" value="BRADI_4g36621v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843091"/>
    <dbReference type="Gramene" id="KQJ91257">
        <property type="protein sequence ID" value="KQJ91257"/>
        <property type="gene ID" value="BRADI_4g36621v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843091"/>
    <dbReference type="OrthoDB" id="1921208at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3PP64">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04216">
        <property type="entry name" value="Phytocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000074">
        <property type="entry name" value="Blue copper binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF179">
        <property type="entry name" value="OS09G0541100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044546095" description="Phytocyanin domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="4">
        <location>
            <begin position="22"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ91257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="172" mass="17970" checksum="15BAB6ABC64EC93E" modified="2016-01-20" version="1">MAATAILLLLFAAAAPRLSGATEYTVGDSDGWTIGPSYLAWSQKYNFTAGDTLVFNYVPRQHDVREVTQDAYRTCEPAANQTVRAWASGRDLVDLAAPGDYYFVCNVTGHCLGGMKFSVAVAAPSPPPPPPPRAFPTPAPPPPSSSSAGAWPDVGGVSGLAIGMWIASLLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3QZ22</accession>
    <name>A0A0Q3QZ22_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g26216v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3QZ22"/>
    <dbReference type="EnsemblPlants" id="KQK06402">
        <property type="protein sequence ID" value="KQK06402"/>
        <property type="gene ID" value="BRADI_2g26216v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK06402">
        <property type="protein sequence ID" value="KQK06402"/>
        <property type="gene ID" value="BRADI_2g26216v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3QZ22"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="155" mass="16431" checksum="7589D40949A2A37B" modified="2016-01-20" version="1">MQQGTGREGGAFQRQRAMGMQRFAVADPSTDRNSAGSKSGRTAGEAGTSAPGNSRWHGHGGKGRGSRRRGRSRMAEVASWMRERGGGGDGGLGVGQQRPRKGSAAGRLARQGREAAHVGGSASLRRKGGDARGRRRQLARSVLLRVEEGLGRYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3RFA3</accession>
    <name>A0A0Q3RFA3_BRADI</name>
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        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">LOC100833245</name>
        <name evidence="4" type="ORF">BRADI_1g00370v3</name>
    </gene>
    <organism evidence="4">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK11898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563471.2">
        <property type="nucleotide sequence ID" value="XM_003563423.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RFA3"/>
    <dbReference type="EnsemblPlants" id="KQK11898">
        <property type="protein sequence ID" value="KQK11898"/>
        <property type="gene ID" value="BRADI_1g00370v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833245"/>
    <dbReference type="Gramene" id="KQK11898">
        <property type="protein sequence ID" value="KQK11898"/>
        <property type="gene ID" value="BRADI_1g00370v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833245"/>
    <dbReference type="OrthoDB" id="659916at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3RFA3">
        <property type="expression patterns" value="differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046527">
        <property type="entry name" value="PIR2-like_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33120">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33120:SF54">
        <property type="entry name" value="PIR2-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12274">
        <property type="entry name" value="DUF3615"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PIR2-like_helical"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="80"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="PIR2-like helical" evidence="3">
        <location>
            <begin position="384"/>
            <end position="496"/>
        </location>
    </feature>
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        <location>
            <begin position="610"/>
            <end position="705"/>
        </location>
    </feature>
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            <begin position="1"/>
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        </location>
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            <end position="67"/>
        </location>
    </feature>
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            <begin position="220"/>
            <end position="247"/>
        </location>
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            <begin position="779"/>
            <end position="800"/>
        </location>
    </feature>
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        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
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        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK11898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK11898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="800" mass="88988" checksum="66E4F8CBC8508069" modified="2016-01-20" version="1">MMSAPPSPGSSGSASSGPSGSASQRSHRPLRRGLISCLHSHTCLHNVAPTGGDEEARTGGDVERPLPDQDLPRAIIKLIVPFYEKAFDRLPCETMPDLVGLLTTGGFCLGLLDPVSNIILNTLALLPEDAAAAASFSPPAGKRSKRVVRNAFPISKRGLCAWHEVAARSYHSLVGFLVAYFGCLTEEQAIRYLYRANANLLLAVMLVQHDLYAEDLHAEGEEPLDPDSDRTRAALESAATSAGHPSPSTLAQVMTIRLKDDDFKLLEYLFSADGTPIMAKDAGAIHHIVMSPLYVTGTSHAIDELAFHVDLAMEAKTEVIPDTSITRTTYSFHSKPIWSMQSGLSKKKLKDCLKTAARQAILLKTPCGDNCDYLRTLNMYLHGMIHNFYIKALKLLPTPSGSLMRSFLTAGHCYGSMDPVSNIIVNSIWYYSRGSSLPLSERRKIDQYIDIFDPVALLPAQVFSLEGLTKLATFVHPQFSVVGCALETLCRAKCNFVEMFTSTTERIEISSIHEAAMAAGHPLPLQLGELHQKLVLMPDERNMLLSLIAGAQTSNTVLPVNEITTFLEAVLCTEPVLGEAPELTSKASRMVSNMRSDYEEKRSWFRSQIEKVLKDYTLKHFWGPKYKLDIILGVEKSWEGPHSRSGRHYCVNFTATSDFKLARELFFAQFWVSSERKPSICCRLPYPYVARCYYGGTTSRKIVYPDRSEYIRVDITIYGTHRVYYMLERDLVYFSPRTILEMDLVYFSPKTDVKLAEYLNHVARLPEVPGHTFGRAGLKQGGCSKIRPRPGQARPSVQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3SD73</accession>
    <name>A0A0Q3SD73_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g70215v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK22938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3SD73"/>
    <dbReference type="EnsemblPlants" id="KQK22938">
        <property type="protein sequence ID" value="KQK22938"/>
        <property type="gene ID" value="BRADI_1g70215v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK22938">
        <property type="protein sequence ID" value="KQK22938"/>
        <property type="gene ID" value="BRADI_1g70215v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3SD73"/>
    <dbReference type="OrthoDB" id="684752at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="102" mass="10917" checksum="257E2E9147B930D0" modified="2016-01-20" version="1">MFPVDEPWRVPDGRSGATDRHDGLQHPLPVVAAGRRSTLYDSFELNAMVGRLNRLLQGDGAGRRPAAPRSLAGTGSWLAAPKVLFRKIKRAFLGGARPAAGS</sequence>
</entry>
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    <accession>A0A2K2CH01</accession>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
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    <gene>
        <name evidence="2" type="ORF">BRADI_5g13354v3</name>
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    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        </location>
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        <location>
            <begin position="84"/>
            <end position="185"/>
        </location>
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        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
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            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT61297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="206" mass="21170" checksum="1F769960A7FC33D7" modified="2018-03-28" version="1">MDSIAGEALLDPNRRQSRRWAQEGDAVRHFARGGYSSAAPRAAPWSGRGRGRGGVAAEEDRWGCGAGGAAARHLGAPVVALDRGEGAGRRGAGRGGAGRNRGGAGPWRRSQRRGRALERRGAAGGGGPGGRQRRHLMRSGLSRTDAGERRRGHQGGGGGVSRGGRTAVVGGGAVWPPESEPTPVRVSSEMAGTRQLAFLPLVGCFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CJ71</accession>
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            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BRADI_5g25228v3</name>
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_5g25228v3"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="695865at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="60"/>
            <end position="99"/>
        </location>
    </feature>
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            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
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        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT62084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="240" mass="28001" checksum="155761CA4D059C29" modified="2018-03-28" version="1" fragment="single">MAGWDDIGLDGPKLLHRLLQADSPKVLQIDACKTKKDKGRNKHKGKISKVTFTQLLEKYKKESEEKSANRPSSVKASRSPQRQKSKDRNWRREKFNLSNSYPPFGPPIPMSWIPPNVDFCSYPSWDKYDSRAHYPSYSRSSHPSYATPRRPFQSKRIERKLQRLSAEELKKKNMTWVPKDKHKDNSDVQAVVARRATKVKKGNNKSNKQFSRSSSAGMIGNPSWIYWESWMHHNSLYHER</sequence>
</entry>
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    <accession>A0A2K2CJB1</accession>
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            <fullName evidence="1 2">Uncharacterized protein</fullName>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CJB1"/>
    <dbReference type="EnsemblPlants" id="PNT62128">
        <property type="protein sequence ID" value="PNT62128"/>
        <property type="gene ID" value="BRADI_5g25885v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62128">
        <property type="protein sequence ID" value="PNT62128"/>
        <property type="gene ID" value="BRADI_5g25885v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CJB1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT62128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="85" mass="9865" checksum="6F75519CD9C91FEF" modified="2018-03-28" version="1">MSMMRKEGLEVSDEVPAMAREREVGGASSEMSFLLLGEIWRRSMLRFPFLKIWRRVCSVVCWCQKPKYGFGTAICPTLWRPLSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMG3</accession>
    <name>A0A2K2CMG3_BRADI</name>
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            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
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    <organism evidence="1">
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        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodium</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CMG3"/>
    <dbReference type="EnsemblPlants" id="PNT63217">
        <property type="protein sequence ID" value="PNT63217"/>
        <property type="gene ID" value="BRADI_4g12967v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63218">
        <property type="protein sequence ID" value="PNT63218"/>
        <property type="gene ID" value="BRADI_4g12967v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63219">
        <property type="protein sequence ID" value="PNT63219"/>
        <property type="gene ID" value="BRADI_4g12967v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63220">
        <property type="protein sequence ID" value="PNT63220"/>
        <property type="gene ID" value="BRADI_4g12967v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT63221">
        <property type="protein sequence ID" value="PNT63221"/>
        <property type="gene ID" value="BRADI_4g12967v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT63217"/>
        <property type="gene ID" value="BRADI_4g12967v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63218">
        <property type="protein sequence ID" value="PNT63218"/>
        <property type="gene ID" value="BRADI_4g12967v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63219">
        <property type="protein sequence ID" value="PNT63219"/>
        <property type="gene ID" value="BRADI_4g12967v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT63220"/>
        <property type="gene ID" value="BRADI_4g12967v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT63221">
        <property type="protein sequence ID" value="PNT63221"/>
        <property type="gene ID" value="BRADI_4g12967v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CMG3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT63220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="124" mass="14140" checksum="F9744D5721CE0D0E" modified="2018-03-28" version="1">MVGQFGEKTSQTKLIKLVHNKLCQLHFHGAQSARAILRGGLHMSAFRSNIFWSICSVSNIWKERNGFIFEAKVPCLSRWVVSLNSGIQLVSIGLESGWKASLRGLHVLFFEEQLLRLGAMWYKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CPD3</accession>
    <name>A0A2K2CPD3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_4g21936v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT64065"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
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    <dbReference type="EnsemblPlants" id="PNT67016">
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        <property type="gene ID" value="BRADI_3g19660v3"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT67016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_010236505.3"/>
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    <dbReference type="STRING" id="15368.A0A2K2CZS3"/>
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        <property type="protein sequence ID" value="PNT67526"/>
        <property type="gene ID" value="BRADI_3g28540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583669"/>
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        <property type="protein sequence ID" value="PNT67526"/>
        <property type="gene ID" value="BRADI_3g28540v3"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34657">
        <property type="entry name" value="EMBRYO SAC DEVELOPMENT ARREST 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34657:SF7">
        <property type="entry name" value="OS10G0472501 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT67526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT67526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="106" mass="10911" checksum="182DDC9D4E50F03F" modified="2018-03-28" version="1">MEVPRGPWRKRKGKDAAGAGPAVPAADAGDRNRLLAGYLAHEFLARGTVRGERRAAGGPGTGSEAGQEQAFVRYEALAALVQHGGARVPGVVNPAQLAAWAAATTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D1V2</accession>
    <name>A0A2K2D1V2_BRADI</name>
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        <recommendedName>
            <fullName evidence="6">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BRADI_3g37900v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="766"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2D1V2"/>
    <dbReference type="EnsemblPlants" id="PNT68260">
        <property type="protein sequence ID" value="PNT68260"/>
        <property type="gene ID" value="BRADI_3g37900v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT68260">
        <property type="protein sequence ID" value="PNT68260"/>
        <property type="gene ID" value="BRADI_3g37900v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="682511at2759"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:1-phosphatidylinositol binding"/>
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    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="OS08G0467300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT68260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="308" mass="33863" checksum="22AB2F978234E582" modified="2018-03-28" version="1">MWALARRAGRTRCWAVALKSLMLAHGLLLRSDELAPGAAARLGRVPFDLADFRARSSGSPGLSAFVRAYFCFLDTRSLFAAQELDAEADDEDARLDRLWKRQHLLELLMQIRPYGDGMEKQSLVLEAMDCVVIEIFEVYSQVCTGIARFLVAVLGSAPTAPRPRRGEDLAAARRRRGAQGMRVLRKAAEQSKQLSAYFELCRGLGVLNAAEFPAVERVPDDDIRDLEKLIMSHHAEDGCKVGDDEAKALVAVEEDDSGVASKTVVTREWVVFDDGDVDGAGARGRQGHFGDYVDPFVGAPWVSVNLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D2X2</accession>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_3g43655v3</name>
    </gene>
    <organism evidence="2">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT68644">
        <property type="protein sequence ID" value="PNT68644"/>
        <property type="gene ID" value="BRADI_3g43655v3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT68644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="70" mass="7312" checksum="EEBF3A30894846F0" modified="2018-03-28" version="1">MEGRAGGHRRGGGRVGVGDPNGPTLWRAEKGTRWGGGRGLGRSGGGIMTVNTVCPFGNFRKVCGFVRPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D2Z4</accession>
    <name>A0A2K2D2Z4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g43772v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT68653.1"/>
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        <source>
            <dbReference type="EMBL" id="PNT68653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="10489" checksum="D8757EF20701640E" modified="2018-03-28" version="1">MTGYGSQWTCKLPYVRRWCPYDWKKCRFGNYPTSTVWISASCKIDLSNWKFSDAAWDRIAHEINSFLDQDLSDLSLSYNWNFSCDLGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D343</accession>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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            <strain evidence="1">Bd21</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT68685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT68685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="10413" checksum="A9B5314AC3CDB807" modified="2018-03-28" version="1">MRRITESFGFLEDGDFTVGYWGSSLIRFSVSVDASRMMNHYKPTRSRWRMSGCSSSWLNSVGLMKVWYVDAHSRFDLVLCIDYSIYSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D5T8</accession>
    <name>A0A2K2D5T8_BRADI</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT69626">
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        <property type="gene ID" value="BRADI_3g59063v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT69626"/>
        <property type="gene ID" value="BRADI_3g59063v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D5T8"/>
    <dbReference type="Proteomes" id="UP000008810">
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT69626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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    </dbReference>
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        <property type="gene ID" value="BRADI_3g59868v3"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT69678.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2 3">Uncharacterized protein</fullName>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="PNT69920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D6L1"/>
    <dbReference type="EnsemblPlants" id="PNT69920">
        <property type="protein sequence ID" value="PNT69920"/>
        <property type="gene ID" value="BRADI_2g02978v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT69920">
        <property type="protein sequence ID" value="PNT69920"/>
        <property type="gene ID" value="BRADI_2g02978v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D6L1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT69920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT69920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="117" mass="12609" checksum="2D77AA6812714CB9" modified="2018-03-28" version="1">MAQFRGVSLDTAGHSELLDDTRVGPSLEAISPFSPPVPSLFLAPARGSSGCGPSRKQQRPSSLLLLRRRRSSPATPHPRTRPPCHPFPSLSSAPARPRRRALLVGSSLPKKRTVRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D7H2</accession>
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    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="1">
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        <lineage>
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            <taxon>Poaceae</taxon>
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            <taxon>Stipodae</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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            <strain evidence="1 2">Bd21</strain>
        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
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        <source>
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        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_2g08290v3"/>
    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT70231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
    <sequence length="82" mass="9132" checksum="C54796F4FE532545" modified="2018-03-28" version="1">MSYHHCYRALPVTHPHGARPCLSPPPRDPSYPNIHPSIHIAGKKSYMHGDFRGLFIASAQQHISVQIKLRRRNNAGAPSTAI</sequence>
</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
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    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
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        <property type="gene ID" value="BRADI_2g31900v3"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="EnsemblPlants" id="PNT71604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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    </evidence>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">cv. Bd21</strain>
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</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGA6"/>
    <dbReference type="EnsemblPlants" id="PNT73300">
        <property type="protein sequence ID" value="PNT73300"/>
        <property type="gene ID" value="BRADI_2g56806v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73300">
        <property type="protein sequence ID" value="PNT73300"/>
        <property type="gene ID" value="BRADI_2g56806v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DGA6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT73300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="9751" checksum="626FE5A30B07E40C" modified="2018-03-28" version="1">MCGRLASRFTARSRLANNDWGQQRIHRRSSSGISNEWRDDKFEASASLFLVVAARGQDVHTVGLRAGGRALDPAECDPGRETKQPLAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGB5</accession>
    <name>A0A2K2DGB5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box/LRR-repeat protein 15-like leucin rich repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100822312</name>
        <name evidence="4" type="ORF">BRADI_2g56920v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024315012.1">
        <property type="nucleotide sequence ID" value="XM_024459244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGB5"/>
    <dbReference type="STRING" id="15368.A0A2K2DGB5"/>
    <dbReference type="EnsemblPlants" id="PNT73315">
        <property type="protein sequence ID" value="PNT73315"/>
        <property type="gene ID" value="BRADI_2g56920v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822312"/>
    <dbReference type="Gramene" id="PNT73315">
        <property type="protein sequence ID" value="PNT73315"/>
        <property type="gene ID" value="BRADI_2g56920v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="423607at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DGB5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22161">
        <property type="entry name" value="F-box_AtSKP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000188">
        <property type="entry name" value="F-box protein SKP2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382:SF69">
        <property type="entry name" value="FI18408P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044576639" description="F-box/LRR-repeat protein 15-like leucin rich repeat domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="3">
        <location>
            <begin position="168"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT73315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="427" mass="46194" checksum="D93E44F3C885E9BA" modified="2018-03-28" version="1">MRSLLAFFIAISDLAGYKSLVMLMNKGRKIRHRSVVFPKVQLSLGYLKKMVNANLMSGNLDNSFSALMVSGGGESGQAQNGGLGTILSGWKDLPMELLLRIISVAGDDRMVVVASGVCTGWRDALGWGVTSLSFSWCQDHMNDLVISLAHKFPKLQVLSLRQIKPQLEDSAVEAVANYCHDLRELDLSRSFRLTDRSLYALAHGCLHLTRLNISGSSNFSDAALVYLTSQCRNLKCLNLCGCVRAASDRALQAIARNCDQLQSLNLGWCDNITDKGVTSLASGCPELRAVDLCGCVLITDESVVALANGCPHLRSLGLYYCQNITDRAMYSLAANSRVRGKGMSWDAGRSSRSKDDKDGLASLNISQCTALTPPAVQAVCDSFPALHTCPERHSLIISGCLSLTSVHCACALHPHRAGRAILSNHAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DJ07</accession>
    <name>A0A2K2DJ07_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g11423v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DJ07"/>
    <dbReference type="EnsemblPlants" id="PNT74257">
        <property type="protein sequence ID" value="PNT74257"/>
        <property type="gene ID" value="BRADI_1g11423v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74258">
        <property type="protein sequence ID" value="PNT74258"/>
        <property type="gene ID" value="BRADI_1g11423v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74257">
        <property type="protein sequence ID" value="PNT74257"/>
        <property type="gene ID" value="BRADI_1g11423v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74258">
        <property type="protein sequence ID" value="PNT74258"/>
        <property type="gene ID" value="BRADI_1g11423v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DJ07"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT74257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="83" mass="8713" checksum="0B19D8C80504B4AE" modified="2018-03-28" version="1">MAGYIGCVASFANRSEGAGDILFSFAWCVGFAALVHAAVCSLAFRGPPLMIGLSFLSHLPHSSLDAITSSGEVSRFCGWNWTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DL01</accession>
    <name>A0A2K2DL01_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Syntaxin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC112270149</name>
        <name evidence="3" type="ORF">BRADI_1g25036v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313688.1">
        <property type="nucleotide sequence ID" value="XM_024457920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DL01"/>
    <dbReference type="STRING" id="15368.A0A2K2DL01"/>
    <dbReference type="EnsemblPlants" id="PNT74956">
        <property type="protein sequence ID" value="PNT74956"/>
        <property type="gene ID" value="BRADI_1g25036v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112270149"/>
    <dbReference type="Gramene" id="PNT74956">
        <property type="protein sequence ID" value="PNT74956"/>
        <property type="gene ID" value="BRADI_1g25036v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="680324at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DL01">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
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        <location>
            <begin position="28"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT74956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="194" mass="21900" checksum="7314E459B9E6E252" modified="2018-03-28" version="1">MSNLPAVASAVGERGIESGRLNTNAASDVQDLFQQIKEFEMLLDNTAVMVHRLKEANREFRSVTEASAVKAIKEHMERNMDEVWKMAHTMTDKLKKMILDSFENKNNAWSEIHGRVTMPMAPSSMTTLIALKNDLQNLRNTIREEYQEVVQIRIFAVSGTKLSDEVIRVIEVGSIVHLGECTSGNNRSREPLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DLU2</accession>
    <name>A0A2K2DLU2_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g29021v3</name>
    </gene>
    <organism evidence="2">
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        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g29021v3"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="PNT75241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="183" mass="20113" checksum="76431EA73E520ED0" modified="2018-03-28" version="1">MPLEVWLLSEGSACQSFLWLCWSWILAGGLVAKVDTFSCWAQVNFSNKWVCPDLSDLVSFHLSGSRHRGCVAAAGKDTRFTGYAALPLCVMSRFFGTTLVLVLGSYRFVLMPCMFYLLSSSDLALYNFVFRPASRFLGLAAHRSSPSRLCLQIGGSARCRSNVCDGTGPLIIRGPKSKFTICT</sequence>
</entry>
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_1g37623v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75741">
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        <property type="gene ID" value="BRADI_1g37623v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DN79"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT75741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="133" mass="15299" checksum="1400A166CCE47973" modified="2018-03-28" version="1">MLIACQVCEPALTHHCSSWIAFPTHTLHMQIMCSVHTRKRSTTVTRSCWLGPLPCVPSQWPRKLSPPIITADVSKITRERMLESMLFGGTRLLVSSLKIMWPCIVSCDTHPQPFLCYYKSVTCRQQRKQHDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DN84</accession>
    <name>A0A2K2DN84_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_1g37565v3</name>
    </gene>
    <organism evidence="9">
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        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
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            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DN84"/>
    <dbReference type="STRING" id="15368.A0A2K2DN84"/>
    <dbReference type="EnsemblPlants" id="PNT75729">
        <property type="protein sequence ID" value="PNT75729"/>
        <property type="gene ID" value="BRADI_1g37565v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT75730">
        <property type="protein sequence ID" value="PNT75730"/>
        <property type="gene ID" value="BRADI_1g37565v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="PNT75731"/>
        <property type="gene ID" value="BRADI_1g37565v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT75732">
        <property type="protein sequence ID" value="PNT75732"/>
        <property type="gene ID" value="BRADI_1g37565v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT75733">
        <property type="protein sequence ID" value="PNT75733"/>
        <property type="gene ID" value="BRADI_1g37565v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75729">
        <property type="protein sequence ID" value="PNT75729"/>
        <property type="gene ID" value="BRADI_1g37565v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75730">
        <property type="protein sequence ID" value="PNT75730"/>
        <property type="gene ID" value="BRADI_1g37565v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75731">
        <property type="protein sequence ID" value="PNT75731"/>
        <property type="gene ID" value="BRADI_1g37565v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75732">
        <property type="protein sequence ID" value="PNT75732"/>
        <property type="gene ID" value="BRADI_1g37565v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75733">
        <property type="protein sequence ID" value="PNT75733"/>
        <property type="gene ID" value="BRADI_1g37565v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DN84"/>
    <dbReference type="OrthoDB" id="692968at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052592">
        <property type="entry name" value="LRR-RLK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48054:SF90">
        <property type="entry name" value="MDIS1-INTERACTING RECEPTOR LIKE KINASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48054">
        <property type="entry name" value="RECEPTOR KINASE-LIKE PROTEIN XA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014294260" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="7">
        <location>
            <begin position="19"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="8">
        <location>
            <begin position="22"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT75733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="242" mass="26594" checksum="C50FC7493E76D3E4" modified="2018-03-28" version="1">MALHILLHISIFLLMCTAKAIDSEAEALLRWRSTLIGAKSVSSWSMANSTCSWSGVTCDAAGHVTRLKLPYAGLSGTLDAFYSEAFRNLISINLGNNNLVGNIPANISLLHTLTFLNLRSNNLVGAIPYQLSHLTSIVGLNLGNNHLTIPEYAKFTPMPNLKFLFLYHNDLNGTFPQYILNCTYSLPEIVPRLRYLDLSANGFSGSIPRSLSRLQKLEVLYLEGNNLTGGIPEELGKITVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DPP3</accession>
    <name>A0A2K2DPP3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g46290v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DPP3"/>
    <dbReference type="EnsemblPlants" id="PNT76245">
        <property type="protein sequence ID" value="PNT76245"/>
        <property type="gene ID" value="BRADI_1g46290v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76245">
        <property type="protein sequence ID" value="PNT76245"/>
        <property type="gene ID" value="BRADI_1g46290v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DPP3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DPP3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT76245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="261" mass="26740" checksum="19331802F47B9D5C" modified="2018-03-28" version="1">MAAAGPLRLRRCSVVRSPARKMATPGGPPSSGCRDALVPRGPSPPPPTTAMPPSTAATDVNDLGISLTSLSLQVTPSPPPPLLAQTHLPEPAPSDLAQVHVPNALETSGPMSGPPPSPSLPSSPRVEDFIAAICTDVPAPVLATPATAVQIVSPTPAVLPPTTVHRRARLAARPLSGISSTLAAQTLVARRLGSLPPAAPFDDEAQEAYVKLFKEPLSEQAIAAIESLVAEARRCKKTPRAAGIGWGMGRGADRGRGIAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DR18</accession>
    <name>A0A2K2DR18_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g52136v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT76721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DR18"/>
    <dbReference type="EnsemblPlants" id="PNT76721">
        <property type="protein sequence ID" value="PNT76721"/>
        <property type="gene ID" value="BRADI_1g52136v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT76721">
        <property type="protein sequence ID" value="PNT76721"/>
        <property type="gene ID" value="BRADI_1g52136v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DR18"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036319273" description="Secreted protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT76721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT76721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="17943" checksum="34627F900D6D9F0A" modified="2018-03-28" version="1">MQVAGLITALASLVLLVLHLACCMMGQPAPRDELNDPLIDQQAVLNNQEEPTNPDLPNNQEEPADVPNNQEEPVDPHLPRNHTAPTLDEIDVEDPVAHRGAQQQEGEEGEQRPDRGSQAQDRLMCVDGLAAGKENCHATDPDERGVDRRRGERCGGVALVELRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DV21</accession>
    <name>A0A2K2DV21_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA topoisomerase</fullName>
            <ecNumber evidence="13">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100833828</name>
        <name evidence="19" type="ORF">BRADI_1g74120v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="13"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229156.1">
        <property type="nucleotide sequence ID" value="XM_010230854.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DV21"/>
    <dbReference type="FunCoup" id="A0A2K2DV21">
        <property type="interactions" value="2324"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DV21"/>
    <dbReference type="EnsemblPlants" id="PNT78130">
        <property type="protein sequence ID" value="PNT78130"/>
        <property type="gene ID" value="BRADI_1g74120v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833828"/>
    <dbReference type="Gramene" id="PNT78130">
        <property type="protein sequence ID" value="PNT78130"/>
        <property type="gene ID" value="BRADI_1g74120v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833828"/>
    <dbReference type="OrthoDB" id="430051at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DV21">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:mitotic cell cycle"/>
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        <property type="project" value="EnsemblPlants"/>
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        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
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        <property type="entry name" value="Topo_IA_Znf"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 13" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
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            <end position="156"/>
        </location>
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        <location>
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        <location>
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    <sequence length="969" mass="107862" checksum="3E9E28AC1965AB0E" modified="2018-03-28" version="1">MHPSGGGGGAIRVLNVAEKPSVAKSVAEILSRRAMQSRPGRSRYNRIFEFNYAIGGQACHMLVTSVTGHLMELDFDDRYRRWYSCDPVELFHAPVRKAVPQDKLDIKRTLEEEARTCQWLVLWLDCDREGENIAYEVVEICAGVNHCLNIWRARFSALIDREIHEAVQHLGRPNKLFADAVDARQEIDLRIGASFTRFQTMLLKDAFVLDVSGEERNMVLSYGPCQFPTLGFIVERFWEIQAHEPEEFWTINCSHTSDEGTASFGWIRGHLFDYSSAIVIYEMCVQEPMATVQNVRNQEKLKYPPYPLSTLELQKRASRCCRMSSEHTMKVAEELYQAGFISYPRTETDNFSPNTDLHAIAHEQVPHPVWGSYAQRLLNPEERLWRNPSNGGHDDKAHPPIHPTKFTAGESNWSQDHHKVYELVVRHFLACCSQPAVGAETTVEIDIAGEQFNASGRVVLAKNYLDVYRYDSWGGSLLPTYTIGQQFVPTTLTLDSGVTRPPPLLAEADLLSCMDKAGIGTDATMHEHIKKLLDRCYATKDANSRFSPTNLGEALVMGYDEMGYELWKPYLRAMMEADMKAVSIGTKSKLEVLEGCLQQMKACFLDARSNKVKLLDAMGTFFARSNRPLNETQNPMEVVRPCGACNDSEMLLKRKPNGGFMVGCRSFPQCRNVVWLPGSLSEAAVTEQICPTCVPGPVYKIQFKFNRRDIPPNFDVDHLGCIGGCDDVLKELIEITRFVNHNQTTTPARGQNQTQTTSGARQGAPRQDLHTGFRSTGQFTNEHTPVVNPQARGQSQTPSGVRQGAPRQDLHPGFRPAGQFANGHTPVVNPQGFRSTHTQNSRNASDPGQVLCTTCGEACISRTANTEANRGRKFYKCQDSGCGFFAWEDELENVVPRGRGRGGRGGGRQASASAGRRGGTQGRGRRGRGRNADANGMTFVSATGDTVHGCCFACGDPSHFANACPNRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DVT3</accession>
    <name>A0A2K2DVT3_BRADI</name>
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        <recommendedName>
            <fullName evidence="16">CCR4-NOT transcription complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100840701</name>
        <name evidence="13" type="ORF">BRADI_1g78550v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="PNT78393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="PNT78393">
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        <property type="gene ID" value="BRADI_1g78550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78393">
        <property type="protein sequence ID" value="PNT78393"/>
        <property type="gene ID" value="BRADI_1g78550v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1933107at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of translation"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ccr4-Not transcription complex subunit"/>
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        <property type="entry name" value="Transcription regulator"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
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        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25097">
        <property type="entry name" value="ARM_Cnot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="11">
        <location>
            <begin position="531"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="10">
        <location>
            <begin position="711"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="9">
        <location>
            <begin position="1025"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="8">
        <location>
            <begin position="1373"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="12">
        <location>
            <begin position="1715"/>
            <end position="1890"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="7">
        <location>
            <begin position="2086"/>
            <end position="2448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1627"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1627"/>
            <end position="1642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="PNT78393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2471" mass="274265" checksum="BE172818FB7A2DE2" modified="2018-03-28" version="1">MNRAPTARAAECRLLAFCLNRQRPADCCIPPPPSPSAPTQRRTRLLPPPLAVCRRDEMLPPSPDVLAAEVRLMLQGATDSNRDSIRRELCQLVDNGLDGCVLLLQVCLDEVLLNAGEEKNFQWKHDLLSAIFRYCLDKTYFCTCFCEAVMMITSTDGLLETLSSVLELSTAEKVGIGLALSDSQNSVMKQKGQQFAIAQIEELCLNPIQSVSNDQIHEIVVFLQQTDGLSKHMDTFSSITSLLEVGQSPFFAPIPKKQRDIQSTNPSRHLDLYFNSTSADFESLLSEIGKEISMADTVTELGYGCTVDIAHCMEMLSAFEPLDNVAISKLLGAVIGNHNSLGEAQNSTHATFVSAIRNSPTSNSPQLTTWNTDVLVDSINAVAPSTNWVQVMENLDHGGFSIPDEAGFYLLMSIYARACKDRFPLHAVCGSMWTNTDGQISFLKHAVSAPATMFTFAHSSRLLALPEFASLSPGNSAWFCLDLLEVLCQLAEVGHTKPVREMLEYPLEHCPELLLVGLGHINTAYNLLQFEVLSCVFPAILKDAAKSNVVNSLWHINTCLTLRGFVDAHSDPSCLLRIVDVCQDMKILSAVLGSTPFAFSIKLAAAASWKDHNHLEKWLTEKLIIYKDSFLKECVNFLKETMSAASYVVEGTMEQPQVSVINKYLEACHPFMKVLQSHLGLLLSNHLSDELRELYTLYESRNHGSVVRDTPTSEGGSDDVEVEANAYFQQMFSGQISIDSMIQMLARFKESPEKREQLIFNCMITNLFEEYKFLTKYPDKQLKLAAVLFGSLIKHQLVAHLGLGIALRAVLDALRKSLDSKMFLFGTIALEQFMDRVVEWPQYCNHILQISHLRENHADMVYAIERALASISSGQNEPNVGNLLSAEQHVSGSSSMETMEVSEPSWQFMGTSPTQLGRTLSSFPLQQRQPEPGVLGDRSMVSMGTSQNNSTLPSQPPVPLTPADSTIDLKATALAHSTNMATTGFLRPRSTPTGLPRQHSYVTGFGAALNIETLVAAAEQRDRPIETPPSEVQDKILFMINNISTSNLEAKANEFNQVLQEQYYPWFAQYMVMKRASIEPNFHELYLKFFAKLNSRSLSKEMLKATYENCKVLLRSDLIKSSSEERSLLKNLGSWLGKFTIGRNQTLRAKEIDPKSLIVEAYEKGLMIAVIPFTSKILEPCQSSIVYRPPNPWTMGILSLLAEIYNLPNLKMNLKFDIEVLFKNLSVDMKDVKPTSLLRDREREVEGNPDFSNKDVASQIPVAAEVSSGIIPPMNHAELQPQVNSTSRAMSLPSILNQYTAPVRLPPNSMAEDDKIASLMPEQVPLHTFTQTPLASLSPSPLSLSQLLSLIPHEEIHFKINTKLGSLGSQLQFSKIIGVALDKAIKEIILPVIERSVTTASKTTKELVLKDYATKSDINSANRSGRLMVGTLAGSLAHVTCKEPLRVALSSHLRSLIQNLTSNSETVDQVTDILINDNLDLGCAIIESVATRQAVDLIDGEITQSFSQQRRKRDAAGPAYYDSFIYAQVPFAPVPEVLRTKPESAAQQRVYEEFVHVWQRRSQSIGAAGSSSASTAAVSSNFGVPRAYSPNSAPATSSTILTSQTAHLTLTQPTELVSEELIPGAAQLSSDSPSQIGTSDSSGWLGGTIASASTSPPPVTSNDLPVGGTTVTADDLSAMMFPPSTISVDNFGLVLPDPLNTDDALKRYRLISQKLEALIAKDGKDSEIQSVIAEVPDLLVKCVSPDEAALAVAQKVFKSLYDNTSNSGRVMWFVATLVAIRDVCKLVVKELTNWVIFSDDEKKFNIEIIIALIRSDLLSLGEYNDYLARLIDGGRNRIATEFAMSLVQKLISQNSVCISELFSVVDALSKISRRPGSPESLQQLIEKSRHKANTAPTFDFGMDEKVRQPKDKKVLSSQTNKEENSVNDITLAESVTFHDQVAHLYTEWCQVCDHPSACDAAYSHFVMQLEHMGLLKGDESTERFIRILTELAVTRSLVSEQIVAPGGLSQQSSQQPHISYFPIDSYSKLVSMVLKHSSVEMGPNEGSLLPKILSVTVKIIQKDAEEKKSLFNPRPFFRLFINWLNDLSSSDPHHDGANFQVLTAFANAFHLLQPLRIPSLSFAWLELVSHRTFMPRLLMCNSQKGWPFFQRLLVDMFKFMEPYLRNADLLEPVRLLYKGTMRVLLVLLHDFPEFLCDYHFSFCDVIPTSCIQMRNVILSAYPHSMRLPDPSTPNLKIDLLAEISIAPRIMSDIGGALKLKHMKTDVDEYLKRPEGSSFLSDIKQKLLLPQNEAIVAGTCYNVPLINSLVLYVGIQAVQLQENKVNALATTPMDIFQIATATEIFRNLATNLDTEGRYLLLNSIANQLRYPNNHTHYFSFIVLYLFAEATQDKIQEQITRILLERLIVKRPHPWGLLITFTELVKNPRYNFWNRSFTHSAPDIQKLFEAAAKSCAAKAAVEGVPDVNRSTMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSG3</accession>
    <name>I1GSG3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S3, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100845739</name>
        <name evidence="10" type="ORF">BRADI_1g21730v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235735.1">
        <property type="nucleotide sequence ID" value="XM_010237433.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235736.1">
        <property type="nucleotide sequence ID" value="XM_010237434.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSG3"/>
    <dbReference type="STRING" id="15368.I1GSG3"/>
    <dbReference type="EnsemblPlants" id="KQK15295">
        <property type="protein sequence ID" value="KQK15295"/>
        <property type="gene ID" value="BRADI_1g21730v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT74776">
        <property type="protein sequence ID" value="PNT74776"/>
        <property type="gene ID" value="BRADI_1g21730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845739"/>
    <dbReference type="Gramene" id="KQK15295">
        <property type="protein sequence ID" value="KQK15295"/>
        <property type="gene ID" value="BRADI_1g21730v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74776">
        <property type="protein sequence ID" value="PNT74776"/>
        <property type="gene ID" value="BRADI_1g21730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845739"/>
    <dbReference type="eggNOG" id="KOG3181">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_2_1_1"/>
    <dbReference type="OMA" id="MYHARKA"/>
    <dbReference type="OrthoDB" id="10248446at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000004">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000006">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="9">
        <location>
            <begin position="20"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK15295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="229" mass="25331" checksum="73A14C039BE0DD4B" modified="2012-06-13" version="1">MAQISKKRKFVADGVFLAELNEMLTRELGEDGFAGVEIRVTPMRTEIIIRATRTQSVLGEKGRRIRELTSVVQKRFNFPDGGVELYAEKVLNRGLCAVAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMISSGHPVNLYIDGAVRHVLLRQGVLGIKVKIMLDWDPKGKQGPATPLPDLVTIHPPKDEDEFLRPLAPQTAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1GU34</accession>
    <name>I1GU34_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100828870</name>
        <name evidence="9" type="ORF">BRADI_1g26550v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003563044.1">
        <property type="nucleotide sequence ID" value="XM_003562996.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GU34"/>
    <dbReference type="STRING" id="15368.I1GU34"/>
    <dbReference type="EnsemblPlants" id="KQK16067">
        <property type="protein sequence ID" value="KQK16067"/>
        <property type="gene ID" value="BRADI_1g26550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828870"/>
    <dbReference type="Gramene" id="KQK16067">
        <property type="protein sequence ID" value="KQK16067"/>
        <property type="gene ID" value="BRADI_1g26550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100828870"/>
    <dbReference type="eggNOG" id="KOG0820">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_2_0_1"/>
    <dbReference type="OMA" id="HIFINPR"/>
    <dbReference type="OrthoDB" id="74991at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000081">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF12">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="8">
        <location>
            <begin position="54"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK16067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="361" mass="39767" checksum="267FE518BF3B3DE4" modified="2012-06-13" version="1">MNRAVSNLRARHVARLGSFCASSSSSSSPASEAWDGRFRLHKPRGQHLLTNPRVLDAIARHAALRPGDAVLEVGPGTGNLTARLLASPAARVSAVEIDPRMVDAVTARASALNLAHKLTVILGDAVETDFPEFDVCVANIPYGISSPLIAKLLFGTYRFRTATLLLQKEFARRLVAKPGDSEYNRLAANVHMVAETRLLMDVSKRDFVPMPRVDSSLVEIRPRVDMPEADLAEWLAFTRECFGQKNKTLGAIFKQKRKILELFKRSHRTKRCADDAPGRGVILGVLDDDNDEACSDDDGNDDNGRSDVVAGFNKEDVGAFKERIANALESTELAGKRPSQLSNDELLRLLRLLNERGVWFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1GZU6</accession>
    <name>I1GZU6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-dependent kinase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100824866</name>
        <name evidence="5" type="ORF">BRADI_1g45985v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDI family. ICK/KRP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560924.1">
        <property type="nucleotide sequence ID" value="XM_003560876.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GZU6"/>
    <dbReference type="STRING" id="15368.I1GZU6"/>
    <dbReference type="EnsemblPlants" id="KQK19020">
        <property type="protein sequence ID" value="KQK19020"/>
        <property type="gene ID" value="BRADI_1g45985v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824866"/>
    <dbReference type="Gramene" id="KQK19020">
        <property type="protein sequence ID" value="KQK19020"/>
        <property type="gene ID" value="BRADI_1g45985v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824866"/>
    <dbReference type="eggNOG" id="ENOG502RZHP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062111_0_0_1"/>
    <dbReference type="OMA" id="ACRARMP"/>
    <dbReference type="OrthoDB" id="695210at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GZU6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045740">
        <property type="term" value="P:positive regulation of DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044275">
        <property type="entry name" value="KRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46776">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="4">
        <location>
            <begin position="217"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="124"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK19020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="265" mass="27568" checksum="4CBE7A24ED15F905" modified="2012-06-13" version="1">MGKYMRKCRAAAGDSSKAPAVPLLGVRTRSRVAAVVGGAAGVAKRRKQATTTTAARAAKELALSGGSDAGCYLHLRSRKLFMASAAVAPPRGLPRAEEEASSAAATLADSGASREAAVAGISRYSSTASSAARERNSGGEAEVCESRDAVESSVSDCCGNRERREATPSSQSPANSSDLESSHAGGEQKHQRTRSPAIRPTTGAATTFQFEERAAKMPPVAEIEEFFAAAEKAEAERFAAKYNFDVALGVPLNAGRFEWTPVASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1H1P4</accession>
    <name>I1H1P4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100827603</name>
        <name evidence="8" type="ORF">BRADI_1g51200v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
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                <consortium name="EnsemblPlants"/>
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        </location>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="166" mass="18065" checksum="1D602972B7ACF7F5" modified="2012-06-13" version="1">MAPDGFGYGYSSTIPIPDDPTLNRYPYPYPWWRFCRHRHQGGWDVSLFWPPPPRRQVFTRGGKDTLEPTPCRLLELGRTPRRYPNRWPDPGGGSGTGGRGQKEQGAAGWPGSGEGDVLGVRVNASSRFPKSEGSGDWLGVGVGVGVACSLSISPAAGRMAEAHWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
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            <taxon>Spermatophyta</taxon>
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            <taxon>Poaceae</taxon>
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    <reference evidence="5 6" key="1">
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">Bd21</strain>
        </source>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
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    <dbReference type="STRING" id="15368.I1H4I3"/>
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        <property type="protein sequence ID" value="KQK21251"/>
        <property type="gene ID" value="BRADI_1g59700v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827197"/>
    <dbReference type="Gramene" id="KQK21251">
        <property type="protein sequence ID" value="KQK21251"/>
        <property type="gene ID" value="BRADI_1g59700v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100827197"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
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        <property type="expression patterns" value="baseline"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF62">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="51"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="142"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="243"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="321"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK21251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="370" mass="41662" checksum="2BDF167CCCB0F679" modified="2012-06-13" version="1">MATPKNWAMAPPYHYHGPEPQPQSQQQEEEAAPAAEGEGGGVAGSGEQQPRSLRIGGLLDWMNEEYLRSCFTRSPELLSAVVKRNKETGKSECFGYLNFADHATADQILQSYNGQKMPNADRDFRLSWVTNYPVQKRDDDGHNIYVGDLAFDVTDFMLHHVFKSRYPSVKHAKIAWDHFNGRSKGYGFVVFGDVNERRQAMTEMNGAYCSTRPMRVGPATKMAGPYTHWTVAGKYSDSDSNNTRLFVGGLDRIVTDEDLKKAFSPYGELTEVKVIAGKKCGFVTYLNRASAEEAMRILNGSLLGDNTIRISWGRSLYHKQKHDQNQWNGERQGSGPGYRSHPEDPKMHGYTGHPEYPHYPQQQAQETSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1H5X2</accession>
    <name>I1H5X2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100835143</name>
        <name evidence="8" type="ORF">BRADI_1g63600v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003557998.1">
        <property type="nucleotide sequence ID" value="XM_003557950.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H5X2"/>
    <dbReference type="FunCoup" id="I1H5X2">
        <property type="interactions" value="275"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H5X2"/>
    <dbReference type="EnsemblPlants" id="KQK21867">
        <property type="protein sequence ID" value="KQK21867"/>
        <property type="gene ID" value="BRADI_1g63600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835143"/>
    <dbReference type="Gramene" id="KQK21867">
        <property type="protein sequence ID" value="KQK21867"/>
        <property type="gene ID" value="BRADI_1g63600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835143"/>
    <dbReference type="eggNOG" id="ENOG502QSIY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035664_8_3_1"/>
    <dbReference type="OMA" id="GHPVMNH"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0006355">
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        <property type="term" value="P:reproductive structure development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="NAC transcription factor 56"/>
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    </dbReference>
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        <property type="entry name" value="NAC domain"/>
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    </dbReference>
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        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF201">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 47"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="9"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="274"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK21867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="347" mass="37758" checksum="4C8D23E0D7F43C41" modified="2012-06-13" version="1">MIMSDPAMLPPGFRFHPTDEELILHYLRNRAAESPCPVSIIADVDIYKFDPWALPSKANYGDREWYFFTPRDRKYPNGVRPNRAAGSGYWKATGTDKPIRSSATSESVGVKKALVFYKGRPPKGVKTNWIMHEYRLAAADAHAGNTYRPMKFRNTSMRLDDWVLCRIYKKTSQMSPMAVPPLSDHEHDEPCAYGGASSAGMVMQGGAAYPGHALAGTQRMPKIPSISELLNEYSLAQLLDEGGAGDIAARPDQHAAFLGHPIMNQYLVNSSGSNNNNMSQLAQTAAGGEGAAGKRKRSEHGGDNGLTSQQAADKKPNGSCFGATTFQIGSNPLQGSLAPGNQRLLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1H8K2</accession>
    <name>I1H8K2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein EARLY HEADING DATE 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein RICE INDETERMINATE 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100834136</name>
        <name evidence="14" type="ORF">BRADI_1g71330v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Transcription activator that acts as a flowering master switch in both long and short days, independently of the circadian clock. Promotes flowering upstream of HD1 by up-regulating FTL1, FTL4, FTL5, FTL6, EHD1, HD3A and RFT1. Seems to repress FTL11 expression. May recognize the consensus motif 5'-TTTGTCGTAAT-3' in target gene promoters.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229031.1">
        <property type="nucleotide sequence ID" value="XM_010230729.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H8K2"/>
    <dbReference type="FunCoup" id="I1H8K2">
        <property type="interactions" value="2113"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H8K2"/>
    <dbReference type="EnsemblPlants" id="KQK23107">
        <property type="protein sequence ID" value="KQK23107"/>
        <property type="gene ID" value="BRADI_1g71330v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834136"/>
    <dbReference type="Gramene" id="KQK23107">
        <property type="protein sequence ID" value="KQK23107"/>
        <property type="gene ID" value="BRADI_1g71330v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834136"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014578_2_2_1"/>
    <dbReference type="OMA" id="NPWERNP"/>
    <dbReference type="OrthoDB" id="6354171at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000554">
        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000131">
        <property type="entry name" value="protein indeterminate-domain 5, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055185">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055186">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031140">
        <property type="entry name" value="IDD1-16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593:SF188">
        <property type="entry name" value="ZINC FINGER PROTEIN GAI-ASSOCIATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22992">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22996">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="197"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="370"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="496"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK23107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="525" mass="55305" checksum="48C3EB88E4AF85BA" modified="2012-06-13" version="1">MEAETSSATAERGVSASGAALLPPPAPPAKKKRALPGMPDPDAEVIALSPKTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLRQRSGKEVRKRVYVCPEPSCVHHDRSRALGDLTGIKKHFCRKHGEKKWKCDKCSKKYAVQSDWKAHAKTCGSREYRCDCGTLFSRRDSFITHRAFCDALAEESAKARVPAAAEEGSSAAAAAPPPVPATPVPLQQLPPAPAQPAPQPQPQHDEQEREFSAAPEPVQFTPPMPSPPSVTGANVSASTSSVSATSQSLLGSIFAPSPVAQAPLQYQERAQAAVVAKPPSLCLTTDASSSLFSAPLADRPPPPSPSPSPHMSATALLQKAAQMGATSSSSSYLRGLGLDISSSSPASTSSGQQHNQHQHQQQEATMHVSFPEGSLPQWPPRLEPEPAPMMSAGLGLGLPYELTGGPVGNPELMMGHQSSLFSAKPATLDFLGLGMSPTGPSASRGLSVFMQPMGGAIGMAGSGAGAAETYGASRGAQAKPWERNPSSSPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1H8K5</accession>
    <name>I1H8K5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100839669</name>
        <name evidence="4" type="ORF">BRADI_1g71350v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024312555.1">
        <property type="nucleotide sequence ID" value="XM_024456787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H8K5"/>
    <dbReference type="FunCoup" id="I1H8K5">
        <property type="interactions" value="588"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H8K5"/>
    <dbReference type="EnsemblPlants" id="KQK23112">
        <property type="protein sequence ID" value="KQK23112"/>
        <property type="gene ID" value="BRADI_1g71350v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839669"/>
    <dbReference type="Gramene" id="KQK23112">
        <property type="protein sequence ID" value="KQK23112"/>
        <property type="gene ID" value="BRADI_1g71350v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839669"/>
    <dbReference type="eggNOG" id="ENOG502QS4V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027536_2_0_1"/>
    <dbReference type="OMA" id="HFITMSP"/>
    <dbReference type="OrthoDB" id="1932894at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02245">
        <property type="entry name" value="cupin_7S_vicilin-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02244">
        <property type="entry name" value="cupin_7S_vicilin-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050253">
        <property type="entry name" value="Seed_Storage-Functional"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189:SF7">
        <property type="entry name" value="OS03G0197300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189">
        <property type="entry name" value="OS03G0336100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094431" description="Cupin type-1 domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="3">
        <location>
            <begin position="36"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="3">
        <location>
            <begin position="260"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="453"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="682"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK23112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="701" mass="80853" checksum="26EFE28C355E0E9C" modified="2012-06-13" version="1">MATATARWLVLLLFVSAASAAAYGEEKWNGGATVGGMVVEKERRRVVAASEAGTVTAADVADDAAGTVYRLQFVTMEPGSLFLPVELHADMVFYVHSGRGKVTYIQEGGSEASALEVERGDVYNLEQGTILYIQSYPNATRERLRIYAIFSSSAISSDDPSHPTSEAYSNVSNLLKGFEVEILRRGFGVPTEVVEPIKSAPSPPLIIPYNPKGKEQESSNWAEEIFDAFWGIRDPQFLNKKKKKSKDKKDKDKKSKDKTFNFYSGEPDVKNCHGWSKTMTNEDLQNLRESNIGMFMVNLTTGSMMGPHWNPKATEIAIVTHGSGIVQTVCPSSPSGEGKRGPHEKGGEEIKCKNSLFRVKEGDVFVVPRFHPMAQMSFNNDSFVFVGFSTHMGQNHPQFLAGKLSALQVIGKEILALSLGQDNSTAVEKLLSAQSDSTILTCVSCAEELEKKVEQEEQEREKEKREREEEERRKKEEEEERKRKEEEEEKARKEEEERRKEEEKARREEEERRRREEEEEKRRKEEERGREEEEEKRRKEEERARQEEEERRRREEEERARREEEERKREEEKAKKEEEERARREQKQEEEERARREQEAQEEAKREQEEIARREQEERARREQEEEERRRREQEGGGGGGGDEPSEEEEGRRKQEEGGRGREDEPRKEDEGGDWGDLQNHTAKKLRKRYSLRKGAVLQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1H9L6</accession>
    <name>I1H9L6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100845630</name>
        <name evidence="5" type="ORF">BRADI_1g74810v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the 3-hydroxybenzoate 6-hydroxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558786.2">
        <property type="nucleotide sequence ID" value="XM_003558738.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H9L6"/>
    <dbReference type="STRING" id="15368.I1H9L6"/>
    <dbReference type="EnsemblPlants" id="KQK23590">
        <property type="protein sequence ID" value="KQK23590"/>
        <property type="gene ID" value="BRADI_1g74810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845630"/>
    <dbReference type="Gramene" id="KQK23590">
        <property type="protein sequence ID" value="KQK23590"/>
        <property type="gene ID" value="BRADI_1g74810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845630"/>
    <dbReference type="eggNOG" id="KOG2614">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_10_2_1"/>
    <dbReference type="OMA" id="CIELKDQ"/>
    <dbReference type="OrthoDB" id="655030at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044560">
        <property type="entry name" value="MOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45934">
        <property type="entry name" value="FAD/NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45934:SF28">
        <property type="entry name" value="OS03G0153100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="21"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK23590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="414" mass="44590" checksum="BC4E5113EE9D1A79" modified="2012-06-13" version="1">MENTYAAATMERSEAAGGGQADIVIVGAGLAGLAVALGLHRKGVKSLVLESSPVLRASGFAFATWPNAFRALDALGVGDKIRKPHLLTQGLRVFSSSTGELIQDVDFTNEGKRNEFRCVRRDVLLQTLEEELPRGAIRYNSKIVSIQEEEGHVKILHLADGSTLRAKVLIGCDGINSVVAKWLGLAKATGSGRAATRGLAHYPDGHGFEPRFLQFIGHGYRAGLIPCNDTDVYWFYTWSHSRNDNGVDGSAAKMKQHVLAELRGSKVPAEAVDVVERSEMSDAAPLRLRPPLSLLWTSISKGNVCVAGDALHPMTPDLGQGGCSALEDGVVLARCLGDAIIHGAGTEKERIESGLREYAGMRRWRSVDLIGTAYVVGFVQQSGNPIVSFLREKVLGGVLARRLVKMSEYDCGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HA22</accession>
    <name>I1HA22_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">serine O-acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100822275</name>
        <name evidence="9" type="ORF">BRADI_1g76140v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homomultimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.30" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558878.1">
        <property type="nucleotide sequence ID" value="XM_003558830.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HA22"/>
    <dbReference type="FunCoup" id="I1HA22">
        <property type="interactions" value="329"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HA22"/>
    <dbReference type="EnsemblPlants" id="KQK23790">
        <property type="protein sequence ID" value="KQK23790"/>
        <property type="gene ID" value="BRADI_1g76140v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822275"/>
    <dbReference type="Gramene" id="KQK23790">
        <property type="protein sequence ID" value="KQK23790"/>
        <property type="gene ID" value="BRADI_1g76140v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822275"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00199"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HA22">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03354">
        <property type="entry name" value="LbH_SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000002">
        <property type="entry name" value="Serine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3130.10:FF:000005">
        <property type="entry name" value="Serine acetyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3130.10">
        <property type="entry name" value="serine acetyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045304">
        <property type="entry name" value="LbH_SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010493">
        <property type="entry name" value="Ser_AcTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042122">
        <property type="entry name" value="Ser_AcTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053376">
        <property type="entry name" value="Serine_acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041874">
        <property type="entry name" value="EPS_EpsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42811">
        <property type="entry name" value="SERINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06426">
        <property type="entry name" value="SATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00971">
        <property type="entry name" value="SATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine acetyltransferase N-terminal" evidence="8">
        <location>
            <begin position="81"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQK23790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="355" mass="37716" checksum="0E306D217F125B37" modified="2012-06-13" version="1">MTSCGCLVLEKVEDHGGGGDAVAAAAARGGGKGRAGCGCGSCAGEWWSRRSFSETVFPIYVMGSSRATAARGILDAAGDPIWEAVKSEAKSEAEKEPILSSFLYASVLSHDCLERALSFVLANRLEDPTLLATQLIDIFNDVMMNDVDIRRSIRLDAQAFKGRDPSCAQYSWALLYLKGYHSLQSYRIAHVLWNQGRKVLALALQSRISEVFAVDIHPAAKIGEGILLDHGTGLVIGETAVIGNWVSLMQGVTLGGTGKEHGDRHPKIGQGALLGAGATILGNINVGEGAMIAAGSLVLKHVPSHSMAVGNPAKVVGYMEKEDPSLTMKHDARRDYFEHVAGSFSDDRPNGSVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I1HB77</accession>
    <name>I1HB77_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100832028</name>
        <name evidence="2" type="ORF">BRADI_2g00780v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565229.1">
        <property type="nucleotide sequence ID" value="XM_003565181.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HB77"/>
    <dbReference type="EnsemblPlants" id="KQK02318">
        <property type="protein sequence ID" value="KQK02318"/>
        <property type="gene ID" value="BRADI_2g00780v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832028"/>
    <dbReference type="Gramene" id="KQK02318">
        <property type="protein sequence ID" value="KQK02318"/>
        <property type="gene ID" value="BRADI_2g00780v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832028"/>
    <dbReference type="eggNOG" id="ENOG502R4AK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102346_0_0_1"/>
    <dbReference type="OMA" id="LTDKHAY"/>
    <dbReference type="OrthoDB" id="662869at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK02318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="265" mass="28877" checksum="1F678AD2384D3044" modified="2012-06-13" version="1">MAGVDGKEELGIILMQAKCASHNIRHNRDRMLQLQLRLQEAPAPGDDDEAARRERLEEVVSGIVDVYFTGIEAGARYLGSCLTMAAENGARLALNPVFALMPDDKLFDALLAQKLPARPTTQVEAFSRVESAFYAVKLPQEHLLPRCVEHLVNIARPATAASEPPKKVSAKTGRRRRRRHGRAPSAPATVATNEPPQDSGTTVEERPRDTSGDMEQARAYLDRACTAVNLAIKHVDLAVMVISSFLDPKDVARFSDLADGGAFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1HBY2</accession>
    <name>I1HBY2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">M-phase phosphoprotein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100838897</name>
        <name evidence="2" type="ORF">BRADI_2g02990v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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            <strain evidence="2 3">Bd21</strain>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <property type="protein sequence ID" value="KQK02671"/>
        <property type="gene ID" value="BRADI_2g02990v3"/>
    </dbReference>
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        <location>
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    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK02671.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="21645" checksum="1B106D420135D331" modified="2012-06-13" version="1">MAARKELSSTLRNLKFMQRAAVAQKIEDKTEVDVEVEAEVLTPAANGGFGSSARVARKCVVVMEGNPHPGAVKGRMSFQNFNPSIDKLNEEASGHQTQSASPSNSQEDGANPSRTDDVSASRFRGFNVDSSESISLNDLKRKEPELEMETPRSQKLPKTTGQNVDGESSSQSNGRGSRKSNKHEKLDWNLLRRRKSK</sequence>
</entry>
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    <accession>I1HDF4</accession>
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        </submittedName>
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            <taxon>Poaceae</taxon>
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            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KQK03365"/>
        <property type="gene ID" value="BRADI_2g07400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834896"/>
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        <property type="gene ID" value="BRADI_2g07400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834896"/>
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    </dbReference>
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        </location>
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        </location>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="218" mass="24655" checksum="E1F1CE28486B65C1" modified="2012-06-13" version="1">MVKGVPKSRPINAAAEVVFDPSVSGPRKPRRAEAPSSSSEYHHFMGSSLTNMYQKPAPEKSTDMSDDEPDIDIEKLLKNVELFGASTWKDRKQIQNRKVVELGGKAIKKQRTPLSVAKPAMKNQQKREQKKIEEERLLGIFRKRDKKDKFQKTRPEDRVLRATEGRFKNGILDVKHLMGGPKPSSSTRDAPEWEARKGKKGGKGKGKGKQKGGRRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1HGH0</accession>
    <name>I1HGH0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-deoxy-manno-octulosonate cytidylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CMP-2-keto-3-deoxyoctulosonic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100825597</name>
        <name evidence="10" type="ORF">BRADI_2g16710v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>3-deoxy-alpha-D-manno-oct-2-ulosonate + CTP = CMP-3-deoxy-beta-D-manno-octulosonate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23448"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:85986"/>
            <dbReference type="ChEBI" id="CHEBI:85987"/>
            <dbReference type="EC" id="2.7.7.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the KdsB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.38" evidence="7"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_2g16710v3"/>
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        <property type="gene ID" value="BRADI_2g16710v3"/>
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    <dbReference type="HOGENOM" id="CLU_065038_2_0_1"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cytidylyl_trans"/>
    </dbReference>
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        <property type="entry name" value="KdsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00466">
        <property type="entry name" value="kdsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003950">
        <property type="entry name" value="PRK05450.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003952">
        <property type="entry name" value="PRK05450.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009905">
        <property type="entry name" value="PRK13368.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42866">
        <property type="entry name" value="3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42866:SF2">
        <property type="entry name" value="3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02348">
        <property type="entry name" value="CTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK04905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="290" mass="31886" checksum="625FB074056B928F" modified="2012-06-13" version="1">MTICAPASESSGSGSRAWVLHGLALGAAAAAAYLYRRPRGFRTLAVGIIPARYASSRFEGKPLALILGKPMIQRTWERVMLASSLDHVVVATDDEKIAECCRGFGADVIMTSVSCQNGSERCCEALQKLEKCYDIVVNIQGDEPLIEPEIIDGVVMALQRAPDAVFSTAVTALKPEDAFDTNRVKCVVDNQGYAIYFSRGLIPFNKSGKANPQFPYHLHLGISCFDSKFLKIYPQLAPTPLQLEEDLEQLKVLENGYRMKVIKVDHDAHGVDAPEDVEKIEALMRARNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HJZ1</accession>
    <name>I1HJZ1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcriptional corepressor SEUSS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100842441</name>
        <name evidence="2" type="ORF">BRADI_2g26970v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568560.1">
        <property type="nucleotide sequence ID" value="XM_003568512.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HJZ1"/>
    <dbReference type="STRING" id="15368.I1HJZ1"/>
    <dbReference type="EnsemblPlants" id="KQK06560">
        <property type="protein sequence ID" value="KQK06560"/>
        <property type="gene ID" value="BRADI_2g26970v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842441"/>
    <dbReference type="Gramene" id="KQK06560">
        <property type="protein sequence ID" value="KQK06560"/>
        <property type="gene ID" value="BRADI_2g26970v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842441"/>
    <dbReference type="eggNOG" id="ENOG502QTWZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007007_0_0_1"/>
    <dbReference type="OMA" id="IHEIMSG"/>
    <dbReference type="OrthoDB" id="774557at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10378">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="568"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="591"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="638"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="667"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="688"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK06560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="887" mass="96135" checksum="EFD98CE2F8C93391" modified="2012-06-13" version="1">MVSSGPPNPMGPGQPVGSASLLRTSSSLLGGGQQGMGAGGGLLQSQSPFSSLVSPRQFGGNGLLGGSSNVSSLLNRPSYGNGAPMSGPGQMPNGVVQMNTLQQRGGLDGAGDLIGAGVSDPASFPSSSQVSLGNHLGSDNLQHQQMEVQDLQHQHERQQQHQLPMSYNQQQLPSQQTQPQVTVKLENGGGMGGVKLEQRIGQHDQNGPSQMMRNAGNVKYEPHHLQALRGLGAVKMEQPNTDSSVFLQQQQQQQQQHHHLLQLTKQNPQAAAAAQLSLLQQQRILQMQQQQKQQQQQQQQQQQQHHMRTPGKPPYEPGTCAKRLTNYMYHQQHRPLDNNIEYWRNFVNEYFAPTAKKRWCVSLYGSGRQTTGVFPQDVWHCEICNRKPGRGFETTVEVLPRLCQIKYASGTLEELLYIDMPRESQNTSGQIVLDYTKAIQESVFDQLRVVREGHLRIVFNPDLKIASWEFCARRHEELIPRRSIIPQVSQLGTVVQKYQAAAQNSASLTTEDMQNNCQSFVQCARQLAKALEVPLVNDLGYTKRYVRCLQIAEVVNCMKDLIDHSRQTGSGPIASLHNFPRRTPSGINPLQSQQQQQQQPEEQQPVPQSSNQSGQNSAAMVGVQASSSANADVTSNNPLSCAPSTSAPSPSVPGLLQGAMDSRQDHPMSNANGLYSNSGNNGAISKVNSTSSLQSNPSTSLPSRGPTTSNNNVISAPQNTNQLSSPGVSSNLPPMQPPPTRPQEPEPSDSQSSVQRILQEMMSSQMNGAGHAGNDMKRSNGLNPGSNGVNCLVGNAVTNHSGMGGMGFGAMGGFGSNPAASGLRMAMANNAMAMNGRMGMHHSAHDLSQLGQQQQHQQQHDIGNQLLGGLRAANSFNSLQYDWKPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1HLQ4</accession>
    <name>I1HLQ4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100844775</name>
        <name evidence="4" type="ORF">BRADI_2g35540v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568911.1">
        <property type="nucleotide sequence ID" value="XM_003568863.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HLQ4"/>
    <dbReference type="EnsemblPlants" id="KQK07450">
        <property type="protein sequence ID" value="KQK07450"/>
        <property type="gene ID" value="BRADI_2g35540v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844775"/>
    <dbReference type="Gramene" id="KQK07450">
        <property type="protein sequence ID" value="KQK07450"/>
        <property type="gene ID" value="BRADI_2g35540v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844775"/>
    <dbReference type="eggNOG" id="ENOG502SC2H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158942_3_1_1"/>
    <dbReference type="OMA" id="MSSTECG"/>
    <dbReference type="OrthoDB" id="650376at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.10">
        <property type="entry name" value="Trypsin Inhibitor V, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000864">
        <property type="entry name" value="Prot_inh_pot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036354">
        <property type="entry name" value="Prot_inh_pot1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091:SF38">
        <property type="entry name" value="OS12G0437800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091">
        <property type="entry name" value="PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00280">
        <property type="entry name" value="potato_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00292">
        <property type="entry name" value="POTATOINHBTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54654">
        <property type="entry name" value="CI-2 family of serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00285">
        <property type="entry name" value="POTATO_INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQK07450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="76" mass="8039" checksum="A07F1E3EE167FA04" modified="2012-06-13" version="1">MSSFSSVDGVACGGGKKTSWPEVVGLSVEEAKKAILKDKPDADIIVLPVGTPVTADFRPNRVRIFIDTVAEIPRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1HML7</accession>
    <name>I1HML7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100834004</name>
        <name evidence="7" type="ORF">BRADI_2g38130v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569047.1">
        <property type="nucleotide sequence ID" value="XM_003568999.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HML7"/>
    <dbReference type="FunCoup" id="I1HML7">
        <property type="interactions" value="484"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HML7"/>
    <dbReference type="EnsemblPlants" id="KQK07869">
        <property type="protein sequence ID" value="KQK07869"/>
        <property type="gene ID" value="BRADI_2g38130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834004"/>
    <dbReference type="Gramene" id="KQK07869">
        <property type="protein sequence ID" value="KQK07869"/>
        <property type="gene ID" value="BRADI_2g38130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834004"/>
    <dbReference type="eggNOG" id="ENOG502QT0N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="WWRWLHD"/>
    <dbReference type="OrthoDB" id="620353at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HML7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044991">
        <property type="entry name" value="TET_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32191">
        <property type="entry name" value="TETRASPANIN-8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK07869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="32693" checksum="B49E9C0877392B3A" modified="2012-06-13" version="1">MMMGGMYTPRFSNVMIGYLNLATLLASIPVIGAGLWLAKGSTTTCSSILQTPLLIIGFVVLLISLAGFVGACFHVAWALWLYLLAVMILIGVLLGLTMFGFAVTAGGGGTQVQGRPYREYHISDYSSWLQKHMQDIKYWKPALACVVGSKACPKISNWTPMDYLQHDLTPSQSGCCKPPTSCTYSGGMPVGAQDEDCFRWNNAPNILCYQCDSCKAGVMEQVRQDWHKISVLNVIVLVFLICVCACGCCAFRNARRSVSEYPYGVNRMSKINPRWDYYWWRWFRDRREQMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I1HNS6</accession>
    <name>I1HNS6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Glyoxylate/succinic semialdehyde reductase 2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100843460</name>
        <name evidence="12" type="ORF">BRADI_2g41630v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH-dependent reduction of glyoxylate to glycolate as well as succinic semialdehyde (SSA) to gamma-hydroxybutyrate in vitro. May function in redox homeostasis and play a role in oxidative stress tolerance by detoxifying glyoxylate and SSA generated in glycolate metabolism and GABA metabolism, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4-hydroxybutanoate + NADP(+) = succinate semialdehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26381"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16724"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.n11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>glycolate + NADP(+) = glyoxylate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.79"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HIBADH-related family. NP60 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569242.1">
        <property type="nucleotide sequence ID" value="XM_003569194.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HNS6"/>
    <dbReference type="FunCoup" id="I1HNS6">
        <property type="interactions" value="2748"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HNS6"/>
    <dbReference type="EnsemblPlants" id="KQK08389">
        <property type="protein sequence ID" value="KQK08389"/>
        <property type="gene ID" value="BRADI_2g41630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843460"/>
    <dbReference type="Gramene" id="KQK08389">
        <property type="protein sequence ID" value="KQK08389"/>
        <property type="gene ID" value="BRADI_2g41630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843460"/>
    <dbReference type="eggNOG" id="KOG0409">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_0_0_1"/>
    <dbReference type="OMA" id="FVPRMTS"/>
    <dbReference type="OrthoDB" id="435038at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HNS6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000016">
        <property type="entry name" value="Glyoxylate/succinic semialdehyde reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000058">
        <property type="entry name" value="Putative oxidoreductase GLYR1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051265">
        <property type="entry name" value="HIBADH-related_NP60_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580:SF2">
        <property type="entry name" value="CYTOKINE-LIKE NUCLEAR FACTOR N-PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580">
        <property type="entry name" value="OXIDOREDUCTASE GLYR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="10">
        <location>
            <begin position="41"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="11">
        <location>
            <begin position="203"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK08389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="34431" checksum="0862BD436F30DCB2" modified="2012-06-13" version="1">MAAAFLLCPRVALPLRRGCRLVVSCSASSSDAPGGVEFQGRVGFLGLGIMGAPMASNLIKAGCDVTVWNRTKSKCDPLLSLGAKFEASPARVASSCDVTFAMLADPESAAEVACGTNGAAEGMAPGKGYVDVSTVDDVTSKLIGERITSTGASFLEAPVSGSKKPAEDGLLIFLTAGDESLYKRVTPLLDVMGKSRFFLGDVGNGAAMKLVVNMIMGSMMVSFAEGLLLSEKVGLDRNTVIEAISQGAINAPMFSLKGPSMVKASYPTAFPLKHQQKDLRLALALAESVSQPIPTAAAANELYKAAKSLGLANQDFSAVIEALKAKVQSSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQ13</accession>
    <name>I1HQ13_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100840731</name>
        <name evidence="12" type="ORF">BRADI_2g45550v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">E2 conjugating enzyme that associates with the E3 ubiquitin-protein ligase EL5 to mediate ubiquitination of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569486.1">
        <property type="nucleotide sequence ID" value="XM_003569438.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232099.1">
        <property type="nucleotide sequence ID" value="XM_010233797.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQ13"/>
    <dbReference type="STRING" id="15368.I1HQ13"/>
    <dbReference type="EnsemblPlants" id="KQK09019">
        <property type="protein sequence ID" value="KQK09019"/>
        <property type="gene ID" value="BRADI_2g45550v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09020">
        <property type="protein sequence ID" value="KQK09020"/>
        <property type="gene ID" value="BRADI_2g45550v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840731"/>
    <dbReference type="Gramene" id="KQK09019">
        <property type="protein sequence ID" value="KQK09019"/>
        <property type="gene ID" value="BRADI_2g45550v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09020">
        <property type="protein sequence ID" value="KQK09020"/>
        <property type="gene ID" value="BRADI_2g45550v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840731"/>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_13_4_1"/>
    <dbReference type="OMA" id="HPNINDR"/>
    <dbReference type="OrthoDB" id="1839788at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000001">
        <property type="entry name" value="Ubiquitin-conjugating enzyme 28, E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="11">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK09019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="147" mass="16532" checksum="8711D1757542ED99" modified="2012-06-13" version="1">MASKRIQKELKDLQKDPPTSCSAGPVGEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNVNSNGSICLDILKDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRHKYESTARTWTQRYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQX3</accession>
    <name>I1HQX3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100820905</name>
        <name evidence="7" type="ORF">BRADI_2g48090v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569675.1">
        <property type="nucleotide sequence ID" value="XM_003569627.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQX3"/>
    <dbReference type="FunCoup" id="I1HQX3">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HQX3"/>
    <dbReference type="EnsemblPlants" id="KQK09455">
        <property type="protein sequence ID" value="KQK09455"/>
        <property type="gene ID" value="BRADI_2g48090v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100820905"/>
    <dbReference type="Gramene" id="KQK09455">
        <property type="protein sequence ID" value="KQK09455"/>
        <property type="gene ID" value="BRADI_2g48090v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100820905"/>
    <dbReference type="eggNOG" id="ENOG502SRMJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073202_1_1_1"/>
    <dbReference type="OMA" id="SGSWMAR"/>
    <dbReference type="OrthoDB" id="693960at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000003">
        <property type="entry name" value="WRKY transcription factor 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF328">
        <property type="entry name" value="OS01G0714800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="6">
        <location>
            <begin position="126"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQK09455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="216" mass="23070" checksum="473B716A88CC522B" modified="2012-06-13" version="1">MAVAAAEAAGTAAYRYHHPHAAADAVACRSMASSPYSSASAAPSFCPAPFNGVQQQLDVLDYFSDDGGPSAVPGTFDTPLQLPPRAPTEAPIVPDVGGYFTAHPRSTAAAAVAAGTTDKIAFRTKSKEEVLDDGYKWRKYGKKSVKNSPNPRNYYRCSTEGCNVKKRVERDRDDADYVLTMYEGIHNHASPGTVYYAAQDATSGRFFVAGTYLSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I1HR73</accession>
    <name>I1HR73_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100836444</name>
        <name evidence="3" type="ORF">BRADI_2g48870v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754269.1">
        <property type="nucleotide sequence ID" value="XM_014898783.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HR73"/>
    <dbReference type="FunCoup" id="I1HR73">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK09581">
        <property type="protein sequence ID" value="KQK09581"/>
        <property type="gene ID" value="BRADI_2g48870v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836444"/>
    <dbReference type="Gramene" id="KQK09581">
        <property type="protein sequence ID" value="KQK09581"/>
        <property type="gene ID" value="BRADI_2g48870v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836444"/>
    <dbReference type="eggNOG" id="ENOG502R58C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023151_5_0_1"/>
    <dbReference type="OMA" id="DFEERYM"/>
    <dbReference type="OrthoDB" id="629734at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HR73">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055302">
        <property type="entry name" value="F-box_dom-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32141:SF96">
        <property type="entry name" value="OS01G0730100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="2">
        <location>
            <begin position="97"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="FBD" evidence="1">
        <location>
            <begin position="368"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK09581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="477" mass="54373" checksum="8CDAAF4C7BDCD9CE" modified="2012-06-13" version="1">MAAAVGDALLRDRLSDVPDSILVAILSLLPIDEAARSTALASRWRHLFPSTLLDFRAPRRNVVKVVDSILATHPTALVRSFSAGRLRFRADQGSAPLDGWLKDLANRGLEELVLQFEERREAIPESILACASLKHLRVISGAFPDVTEAAASPLARLTKIELSAVKISDESVESLLSQCTALEHLEMNGVSNCGCLRIRSRSLKVLNSHGDFDELFIEDAPNLERVLGNFMHLRKVHIEVKHAPKLEFLGYLSMLHTIEIGGTELLSIKEVEAENEDDDEDEDEEIRPPRKFTEVRNYVKTMPSMKTLAVSVSYMTRGYYTIEGFIGWFMELLKLFPCLETLYIKSESWSGVDEDEVPGSWDVLRSVPCINNHLEKVVLEVYRGHDWQMEMAKFFHGRSRFLKTMEFHCMDDTGRADYGKAPSEEWVGNQHELLCFDSRVSSDARFVFFKHQLPCNHHYICHDEDYKRDYYSNLYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSP6</accession>
    <name>I1HSP6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100826325</name>
        <name evidence="8" type="ORF">BRADI_2g53027v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564453.1">
        <property type="nucleotide sequence ID" value="XM_003564405.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSP6"/>
    <dbReference type="STRING" id="15368.I1HSP6"/>
    <dbReference type="EnsemblPlants" id="KQK10259">
        <property type="protein sequence ID" value="KQK10259"/>
        <property type="gene ID" value="BRADI_2g53027v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826325"/>
    <dbReference type="Gramene" id="KQK10259">
        <property type="protein sequence ID" value="KQK10259"/>
        <property type="gene ID" value="BRADI_2g53027v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826325"/>
    <dbReference type="eggNOG" id="ENOG502QUZX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051752_0_0_1"/>
    <dbReference type="OMA" id="CCYSFAT"/>
    <dbReference type="OrthoDB" id="1849707at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF70">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN LEA-2 SUBGROUP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="7">
        <location>
            <begin position="166"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK10259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="299" mass="31923" checksum="DCDE33B4DDD79BDA" modified="2012-06-13" version="1">MMSDGRVHPAAATSSDFSGEMNHSATYSSSDPSSSPLYSFHFEKPIPPPPQTHAPLQQQQPPHKPLPGTYVVQVPKDKVFRVPPPENARLFQHYARRAKRRRSGCFSSCCLCLFLALLALAILLAALAGVAYLALQPQKPSYAVRSLAVSGIGNASVALAPRFDATIRAENPNGKIGVRYDAGSGMSVSYGGVSLAAGAWPAFYQAPRSATVFVATAKGAGIRLSERAQGQMAAAERLRSVPLDVELKVPVRLQIGALKTWAMPVTVRCAVAVDRLAVDAKVVSAGPCDVDARSLFWRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1HSV5</accession>
    <name>I1HSV5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">trans-cinnamate 4-monooxygenase</fullName>
            <ecNumber evidence="12">1.14.14.91</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome P450 73A33</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100839986</name>
        <name evidence="17" type="ORF">BRADI_2g53470v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(E)-cinnamate + reduced [NADPH--hemoprotein reductase] + O2 = (E)-4-coumarate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15669"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.91"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:10609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.91" evidence="12"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564495.1">
        <property type="nucleotide sequence ID" value="XM_003564447.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HSV5"/>
    <dbReference type="STRING" id="15368.I1HSV5"/>
    <dbReference type="EnsemblPlants" id="KQK10342">
        <property type="protein sequence ID" value="KQK10342"/>
        <property type="gene ID" value="BRADI_2g53470v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839986"/>
    <dbReference type="Gramene" id="KQK10342">
        <property type="protein sequence ID" value="KQK10342"/>
        <property type="gene ID" value="BRADI_2g53470v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839986"/>
    <dbReference type="eggNOG" id="KOG0156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_4_0_1"/>
    <dbReference type="OMA" id="DPRAYWQ"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HSV5">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016710">
        <property type="term" value="F:trans-cinnamate 4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009808">
        <property type="term" value="P:lignin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11074">
        <property type="entry name" value="CYP73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000013">
        <property type="entry name" value="Trans-cinnamate 4-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47948">
        <property type="entry name" value="TRANS-CINNAMATE 4-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47948:SF4">
        <property type="entry name" value="TRANS-CINNAMATE 4-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15 16" id="KW-0349">Heme</keyword>
    <keyword evidence="9 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KQK10342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="505" mass="57957" checksum="4727F7743E8A7867" modified="2012-06-13" version="1">MDFLFVEKLLVGLLASALVAIAVSKIRGRKLKLPPGPLPVPIFGNWLQVGDDLNHRNLAAMARKFGEIFLLRMGQRNLVVVSSPPLAREVLHTQGVEFGSRTRNVVFDIFTGEGQDMVFTVYGDHWRKMRRIMTVPFFTNKVVQQYRAGWEAEAAFVVDNVRADPRAATDGVVLRRHLQLMMYNNMYRIMFDRRFESLDDPLFLRLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLRLCKEVKETRLKLFKDYFLEERKKLASTKAMDNNGLKCAIDHILEAQQKGEINEDNVLYIIENINVAAIETTLWSIEWAIAELVNHPEIQQKLRDELDTVLGAGHQITEPDTHKLPYLQAVIKETLRLRMAIPLLVPHMNLQDAKLGGYNIPAESKILVNAWFLANNPEEWRRPDEFRPERFLEEEKHVEANGNDFRFLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQDKLDTTEKGGQFSLHILKHSNIVAKPRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZM7</accession>
    <name>I1HZM7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100821019</name>
        <name evidence="10" type="ORF">BRADI_3g10620v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572668.1">
        <property type="nucleotide sequence ID" value="XM_003572620.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HZM7"/>
    <dbReference type="STRING" id="15368.I1HZM7"/>
    <dbReference type="EnsemblPlants" id="KQJ94454">
        <property type="protein sequence ID" value="KQJ94454"/>
        <property type="gene ID" value="BRADI_3g10620v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821019"/>
    <dbReference type="Gramene" id="KQJ94454">
        <property type="protein sequence ID" value="KQJ94454"/>
        <property type="gene ID" value="BRADI_3g10620v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821019"/>
    <dbReference type="eggNOG" id="KOG0001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023603_0_0_1"/>
    <dbReference type="OMA" id="HMGKGSK"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048579">
        <property type="term" value="P:negative regulation of long-day photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000307">
        <property type="entry name" value="Phosphatidylinositol 4-kinase gamma 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044571">
        <property type="entry name" value="P4KG1-8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800:SF3">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE GAMMA 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="32"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="109"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="9">
        <location>
            <begin position="257"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ94454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="563" mass="61717" checksum="DE60C632AC285FC0" modified="2012-06-13" version="1">MSAAGVATLSPLLDQLAYVPNCLHRLEPLDSILIFLAMPGTPPMPMRVLRSESIASVKLRIQRSNGFVVTKQRLVFSGHELSCNNSHVRDCGLADGNVLHLVLRLADLRSITIKTASGKKFKYQMASGSNVGYLKKKLAAETGEQLERLEDQRLVCDGEELEDNQLITDICRKGAAVIHLFIRTPAKVKTQQIDKDTVVTVVNPEGSDNFQIDALDLTKPASGVHPPVEPIIVNRKVELSPAVMKMISSTRAGLDNGFLPVMSTEGSGGVYFMQDPSGHSNVAVFKPIDEEPMAENNPRGFPFSVDGEGLKRGTRVGEGALREVAAYILDHPIDGCKSSEATGFSGVPPTALVRCSHKGKGFKIGSLQIFVNNHGSCEDMGPRDFPVQEVQKIAVLDIRLANADRHAGNILVCRDGEDHLKLVPIDHGYCLPEKFEDCTFEWLYWPQARESFSAETTAYIASLDADKDIALLKFYGWNLSPQCARVLCISTMLLKKGAERGLAPYDVGSILCRKTANKESEIEGIINEAEDAVLPETSEEMFLEAVSEIMDRHLDNMLSKLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1I124</accession>
    <name>I1I124_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">D-aminoacyl-tRNA deacylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100844903</name>
        <name evidence="4" type="ORF">BRADI_3g15390v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573407.1">
        <property type="nucleotide sequence ID" value="XM_003573359.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I124"/>
    <dbReference type="FunCoup" id="I1I124">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I124"/>
    <dbReference type="EnsemblPlants" id="KQJ95141">
        <property type="protein sequence ID" value="KQJ95141"/>
        <property type="gene ID" value="BRADI_3g15390v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844903"/>
    <dbReference type="Gramene" id="KQJ95141">
        <property type="protein sequence ID" value="KQJ95141"/>
        <property type="gene ID" value="BRADI_3g15390v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844903"/>
    <dbReference type="eggNOG" id="ENOG502QRIE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056464_0_0_1"/>
    <dbReference type="OMA" id="IGKPNNF"/>
    <dbReference type="OrthoDB" id="192183at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10700:FF:000001">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.50:FF:000001">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10700">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.50">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018033">
        <property type="entry name" value="Deacylase_DtdA_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007508">
        <property type="entry name" value="DtdA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667:SF1">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04414">
        <property type="entry name" value="tRNA_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016210">
        <property type="entry name" value="UCP016210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142535">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ95141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="33696" checksum="72A482CCE9C5C234" modified="2012-06-13" version="1">MVVLVVATASDPASIGPASAFLAMPGWSPGPPIPEGMESFTNGNIRLLKHDRSIIAEDNLDHRWQEATGEAISEVIFLSKHTAVSNRPALTVHPIGVPHLREDETPPQGGRPGWAALPNPRIGPWLRLMQQIAADQGLVPEFEITLEATHHGPLTNTPTMFVEIGSTEEYWGRQDAAQAIALVLWKGLGLEEGKAVGTWTGNGEKVLLGIGGGHYAPRHMDIVIKNGAWVGHLLSGYSLPMEAPPEGNSKGSGVGGMWKHSIKASFEATKAAFPGGEVIAHLDQKSFKGWQKNAITSYLQEENIRIGKPNDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1I1E0</accession>
    <name>I1I1E0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100835128</name>
        <name evidence="9" type="ORF">BRADI_3g16377v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ95303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573458.1">
        <property type="nucleotide sequence ID" value="XM_003573410.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I1E0"/>
    <dbReference type="STRING" id="15368.I1I1E0"/>
    <dbReference type="EnsemblPlants" id="KQJ95303">
        <property type="protein sequence ID" value="KQJ95303"/>
        <property type="gene ID" value="BRADI_3g16377v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835128"/>
    <dbReference type="Gramene" id="KQJ95303">
        <property type="protein sequence ID" value="KQJ95303"/>
        <property type="gene ID" value="BRADI_3g16377v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835128"/>
    <dbReference type="eggNOG" id="KOG1677">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060653_1_0_1"/>
    <dbReference type="OMA" id="EMWATEE"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000016">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000045">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF156">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="240"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="240"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="288"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ95303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ95303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="362" mass="38696" checksum="0BE826DBCE79C120" modified="2012-06-13" version="1">MDYSNAIHIIPDAAGPEAWVNSAVLPGGDSGIWATEDDYSQWNAEPGLTGGGYGGDRNSSQPQSRSGSEQPPPGKKPRGGPSGGGGGDAGSMSKSRAIGKMFFKTKLCCKFRAGTCPYVTNCNFAHGMEELRKPPPNWQEIVAAHEEATEQREEHQIPIMTSSSVAPSESVSGRAYKGRHCKKFYTEEGCPYGDACTFLHDEQSKARESVAISLSPSVGGGSYNSPTAAANGPTILKPSNWKTRICNKWEMTGYCPFGSKCHFAHGAAELHKYGGGLVDVEGRDIASTPDSKQAALSAKAPAETTPASTAAPPHSDVYHLGIQSQRSTMTSQRSGQLPRPIQKWKGPDKISRIYGDWIDETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1I4P2</accession>
    <name>I1I4P2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exportin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100839622</name>
        <name evidence="9" type="ORF">BRADI_3g28760v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574058.1">
        <property type="nucleotide sequence ID" value="XM_003574010.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I4P2"/>
    <dbReference type="FunCoup" id="I1I4P2">
        <property type="interactions" value="1460"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I4P2"/>
    <dbReference type="EnsemblPlants" id="KQJ97088">
        <property type="protein sequence ID" value="KQJ97088"/>
        <property type="gene ID" value="BRADI_3g28760v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839622"/>
    <dbReference type="Gramene" id="KQJ97088">
        <property type="protein sequence ID" value="KQJ97088"/>
        <property type="gene ID" value="BRADI_3g28760v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839622"/>
    <dbReference type="eggNOG" id="KOG4541">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005818_1_0_1"/>
    <dbReference type="OMA" id="SCKSIFH"/>
    <dbReference type="OrthoDB" id="5548448at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000287">
        <property type="entry name" value="Exportin-4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044189">
        <property type="entry name" value="XPO4/7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596">
        <property type="entry name" value="EXPORTIN 4,7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596:SF1">
        <property type="entry name" value="EXPORTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ97088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1160" mass="129500" checksum="E65AD95B48FB74B2" modified="2012-06-13" version="1">MQNFPGGAPDPQQLQATMLAIEQACSLIQVHMNPSEAEKVLSSLHSSLMPYQSCRFILETSLMPNARFQAAGAIGDAAIREWGILTDDNKRSLILYCLNYVMEHAGSPDGYVQSKVSAVAARLLKRGWLEFPDQEKGAIFFEVEQSVRGIHGPNRQFAGINFLETLVSEFSPSTASSMGLPKEFHEQCQWSLEVKFLKDFYCWAQAAVFNTTDTILNSNVTTPEEKACSAALRLMLQILSWSFKQALEHENSDAKINSGLRSDAINLKKFERSLVKPGSTWTDILISSGHTTWVLNFYTTLRQKYLYDTLWGDSPIAVSCRQLVVQLCSLAGAVFPDDKGDAQIEHFMHILSAVILWIEPPGVIAESIRSGGSESEFIDGCHALLSVASLTSSSLFDNLLKSIRQYGTINLLSALTSEAVKSVLNNQNEEETWGSDALDILLETWSVILGEADADRSPMSVDGALAASSLFKIIVESHLKAAADSAFEDSDDAEYFHVSVSKRDEQLALYALIARAAADTTIPFLEQLFSERFVQLSQRNGENDPTRTLEELYWLLLITSHVLTDSGEGETLLIPEALQAGFPNVVEATQHPVVTLSWSIINFSRQCLDPGIRGSYFSPRLMEAVIWFLARWVGTYLVPLDVSREIDSMGKHRSQQSRKLLNSFAWENNQGELVLDFVVLISMVALTTYQGEIELQTLICQKLLATVVRRKHTCTYVVQLDSWRDLTRAFASGRSLFSLSGRLQRSLAETLACAASCIKDPEASVQYLRDLMGPVAGCLVENASRSDLKSVAHQPDVIYMICCLLERLRGAARATQPRTQKVLFEMGRTVMNSLLTLLEVYKNQSQVIYMILKFVVDFIDGQAVFLDAKETSVLMSFCLRLLQIYSSHNIGKVMLSLSSTLRSESQSEKYKDLRALLRLLTNICSKDLVGFLSDSNIEGSPDIAEVIYVGLDIVTPLVSLDLLKYPKLSRDYFALMSHLLEVYPEKVAHLNRDAFARIIGSLEFGLRNQDSDVVERCLTAVNALASYHFKERLGGRGGLSSQLMESEGSNGKLQESISSHFLRLLMQLLLFEDFRMELAGSAADALLPLLFCEQELYQRLVHELLEKQQNPTIKSRLAVAFHNLTSCNNLSSTLDRPNRQKFRKNLRAFLVEISGFMQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5P9</accession>
    <name>I1I5P9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100823283</name>
        <name evidence="6" type="ORF">BRADI_3g32080v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003574257.1">
        <property type="nucleotide sequence ID" value="XM_003574209.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5P9"/>
    <dbReference type="STRING" id="15368.I1I5P9"/>
    <dbReference type="EnsemblPlants" id="KQJ97591">
        <property type="protein sequence ID" value="KQJ97591"/>
        <property type="gene ID" value="BRADI_3g32080v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823283"/>
    <dbReference type="Gramene" id="KQJ97591">
        <property type="protein sequence ID" value="KQJ97591"/>
        <property type="gene ID" value="BRADI_3g32080v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823283"/>
    <dbReference type="eggNOG" id="KOG0424">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_12_1_1"/>
    <dbReference type="OMA" id="TWECGIP"/>
    <dbReference type="OrthoDB" id="6600758at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061656">
        <property type="term" value="F:SUMO conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000038">
        <property type="entry name" value="SUMO-conjugating enzyme SCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="6"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ97591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="161" mass="18046" checksum="8EA63465264AE0C3" modified="2012-06-13" version="1">MSGGGIARGRLAEERKAWRKNHPHGFVAKPETVADGSVNLMVWHCTIPGKQGTDWEGGYFPLTLQFSEDYPSKPPKCKFPQNFFHPNVYPSGTVCLSILNEDSGWRPAITVKQILVGIQDLLDQPNPADPAQTDGYHLFIQDPAEYKKRVRLQAKQYPALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1I6E5</accession>
    <name>I1I6E5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Armadillo repeat-containing domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100835554</name>
        <name evidence="1" type="ORF">BRADI_3g34120v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
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            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="BRADI_3g34120v3"/>
    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="19536" checksum="B3D80A3FA51473CA" modified="2012-06-13" version="1">MFTNAQRQVERTGRGGTPRNQYLQDLVSQFQDSTDEESKEKIVANLANFAYDPYNYAFMRQLNILELFLDCITEPNERLIEFGIGGICNSCVDPANASVITQCGGIPLVVQCLSSPVRNTVNYALGALYYLCNPSTKKEILRPDVLRVIGDYATVGAVNSSFNNLANTFLDKHVNP</sequence>
</entry>
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    <accession>I1I8U0</accession>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003574759.3"/>
    </dbReference>
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    <dbReference type="FunCoup" id="I1I8U0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ99084">
        <property type="protein sequence ID" value="KQJ99084"/>
        <property type="gene ID" value="BRADI_3g40960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839232"/>
    <dbReference type="Gramene" id="KQJ99084">
        <property type="protein sequence ID" value="KQJ99084"/>
        <property type="gene ID" value="BRADI_3g40960v3"/>
    </dbReference>
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    <dbReference type="eggNOG" id="KOG4374">
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        <property type="entry name" value="OS08G0526500 PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR10627">
        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ99084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="224" mass="24542" checksum="7EBB3F1ADBEB3B57" modified="2012-06-13" version="1">MYADKVSGRKRSIMDRLGSGGGSRPRSESAKRFRQVDGTWRRELYKDSEGNQISSGLASRNLQAHQKSHLKQSTEVVKKSSVPDLREKLSGVQRPQLNSMVQIPKPAVTEIAKSAKPVQKRELSAPAPSAAAAALPATKNVNAPTAPKQSQEKADSSLDRLLKSLDLEKYSINFQAEEVDMKALVHMNEEDMKSLGIPMGPRKKILSALASKRKKSSRSLPPTS</sequence>
</entry>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
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    <reference evidence="2 3" key="1">
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ99284.1"/>
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    <dbReference type="Gramene" id="KQJ99284">
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        <property type="gene ID" value="BRADI_3g42310v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R3T3">
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ99284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="117" mass="12398" checksum="5FFEF05416F7B57E" modified="2012-06-13" version="1">MEIKVERRKRMGGEGDGFGCFFTEAELAAADQLVQLSASGEATAASASSPTSSTLSVNKGGGEAAARGTTLAAAEDDEDAAAWPMDRRARKRYRTVAELYEATRPAKRGGGSKRRNR</sequence>
</entry>
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    <accession>I1ID17</accession>
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        <recommendedName>
            <fullName evidence="13">ZF-HD dimerization-type domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homo- and heterodimer with other ZFHD proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572860.1">
        <property type="nucleotide sequence ID" value="XM_003572812.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ID17"/>
    <dbReference type="STRING" id="15368.I1ID17"/>
    <dbReference type="EnsemblPlants" id="KQK00949">
        <property type="protein sequence ID" value="KQK00949"/>
        <property type="gene ID" value="BRADI_3g52837v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838704"/>
    <dbReference type="Gramene" id="KQK00949">
        <property type="protein sequence ID" value="KQK00949"/>
        <property type="gene ID" value="BRADI_3g52837v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838704"/>
    <dbReference type="eggNOG" id="ENOG502QS6S">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_643092_0_0_1"/>
    <dbReference type="OMA" id="HMQKRPP"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Zinc-finger homeodomain protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
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    <dbReference type="InterPro" id="IPR006455">
        <property type="entry name" value="Homeodomain_ZF_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006456">
        <property type="entry name" value="ZF_HD_homeobox_Cys/His_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01565">
        <property type="entry name" value="homeo_ZF_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01566">
        <property type="entry name" value="ZF_HD_prot_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948">
        <property type="entry name" value="ZINC-FINGER HOMEODOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31948:SF138">
        <property type="entry name" value="ZINC-FINGER HOMEODOMAIN PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04770">
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    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51523">
        <property type="entry name" value="ZF_HD_DIMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZF-HD dimerization-type" evidence="13">
        <location>
            <begin position="194"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="98"/>
            <end position="173"/>
        </location>
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            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="414"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
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            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="252"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
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            <begin position="335"/>
            <end position="354"/>
        </location>
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            <end position="452"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK00949.1"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK00949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="452" mass="48373" checksum="AAF2B64D7FDF3BE5" modified="2012-06-13" version="1">MEYKRSSHVEEDDDMEEEDEMEEDDDEEEEEEEDEEEEMKGRHHHHHQHHYAAAAPAATGGAPPPHQHAHGHVLVSSAAASSPSSLLESAAFSRPLLPPNSSLISSPPGFLSPHDHHHHQQQQQPHRRRGERERERTVESTGEQQRQRQQEPARNGVLGGGSGGPMPPPAAPAALALVTGGSAAAAGEPLPWRYRECLRNHAARLGAHVLDGCGEFMPSSAPGEGSAAAAALACAACGCHRSFHRREPVVAAASPSPSPASAVVSPSATPRAGAGAGANPNSRLIPLLLAPPHMQQQKRPPVVVPGSSPMSAPAALAVAESSSEELPRPPPQQQHPPHAQAAAMAGSASAPAAPGKKRFRTKFTPEQKDRMLEFAHRVGWRIHKPDGGAVDAFCDQVGVSRRVLKVWMHNNKHLAKTSPSPSQPLPPLHHDPPPAPHHHHHHPAQHHQQHDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I1IDZ4</accession>
    <name>I1IDZ4_BRADI</name>
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        <recommendedName>
            <fullName evidence="5">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQK01383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003570363.4"/>
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        <property type="gene ID" value="BRADI_3g55530v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836870"/>
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        <property type="gene ID" value="BRADI_3g55530v3"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
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        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
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        <property type="entry name" value="Thioredoxin_domain"/>
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    <dbReference type="InterPro" id="IPR044253">
        <property type="entry name" value="WCRKC1/2"/>
    </dbReference>
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        <property type="entry name" value="THIOREDOXIN-LIKE 3-2, CHLOROPLASTIC"/>
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    </dbReference>
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        <property type="entry name" value="THIOREDOXIN-LIKE 3-2, CHLOROPLASTIC"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Thioredoxin-like"/>
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    </dbReference>
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        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="59"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK01383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="195" mass="21179" checksum="8215ED86F1976A4E" modified="2012-06-13" version="1">MATASVSAYAAATGAGGEAAAAAAAAGLSRARARARPPPPRLALLSRSPPARWVSSISLRRGLHLAAAAAEAREEEGPAWVELEPVGSEQQFDRVLAEAQKRGVPLVVLWTASWCRKCIYLKPKLEKLAAEYYPRIQFYCVDVNAVPQKFVNRAGVTKMPAIQMWSDSLKQAEVIGGHKSWLVINDVRSMIEQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1ILA8</accession>
    <name>I1ILA8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone chaperone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100823815</name>
        <name evidence="6" type="ORF">BRADI_4g16847v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577493.1">
        <property type="nucleotide sequence ID" value="XM_003577445.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ILA8"/>
    <dbReference type="STRING" id="15368.I1ILA8"/>
    <dbReference type="EnsemblPlants" id="KQJ88294">
        <property type="protein sequence ID" value="KQJ88294"/>
        <property type="gene ID" value="BRADI_4g16847v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100823815"/>
    <dbReference type="Gramene" id="KQJ88294">
        <property type="protein sequence ID" value="KQJ88294"/>
        <property type="gene ID" value="BRADI_4g16847v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100823815"/>
    <dbReference type="eggNOG" id="ENOG502QQUQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026346_0_0_1"/>
    <dbReference type="OMA" id="APTVYKK"/>
    <dbReference type="OrthoDB" id="514832at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1ILA8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037647">
        <property type="entry name" value="HIRIP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019098">
        <property type="entry name" value="Histone_chaperone_domain_CHZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410:SF2">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09649">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01082">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone chaperone" evidence="5">
        <location>
            <begin position="391"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="72"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="198"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="371"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="208"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="287"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="428"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ88294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="469" mass="51716" checksum="6ADCEF6564A13634" modified="2012-06-13" version="1">MAADDAAAAAFAVREAEIEEALRARISLFKEQADSLTLEGVRRTLEKDMDLELHSLDAHKKFIKQLVEKVFSDSDDEDANNGSEKAEAKDHINSSKEVPEDAQPMSGSNKISSGADEQGARSSKTEEDPEGTKNQTYGSDISEAEIKKAISKRASYFRENSGTLTLQGARRTLEEDLKLEKKALDAYKVFISTELDKVLLEPANGTTEHNKKESRKDADGKTSKGSKRARQDSDTSVISDSHREREDSDEDTRPKKRGSEKGKAIKRQKKVTVEKKMSTSKVKKVAKRDLDKSSKKQGGDAEEDSSHSSAEEDSKKKQQPATVYGRRVERLKCVIKSCGMSIPPSTYRRAKQAPESNREACLIKELEDILEKEGLSSNPSEKEIKAVKKRKERAKDLEGIDMSNIITSSRRRSASSFIPLPMPRFEADSDDDDEDLAEDDDNVVGGDKGDNGDSEAGDGSTDDAGDKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I1IQH2</accession>
    <name>I1IQH2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Subtilisin-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100844401</name>
        <name evidence="18" type="ORF">BRADI_4g31360v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 11">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010238218.1">
        <property type="nucleotide sequence ID" value="XM_010239916.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IQH2"/>
    <dbReference type="FunCoup" id="I1IQH2">
        <property type="interactions" value="951"/>
    </dbReference>
    <dbReference type="MEROPS" id="S08.A24"/>
    <dbReference type="EnsemblPlants" id="KQJ90410">
        <property type="protein sequence ID" value="KQJ90410"/>
        <property type="gene ID" value="BRADI_4g31360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844401"/>
    <dbReference type="Gramene" id="KQJ90410">
        <property type="protein sequence ID" value="KQJ90410"/>
        <property type="gene ID" value="BRADI_4g31360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844401"/>
    <dbReference type="eggNOG" id="ENOG502QRTY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000625_4_6_1"/>
    <dbReference type="OMA" id="FSTHHQW"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10 11" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095469" description="Subtilisin-like protease" evidence="13">
        <location>
            <begin position="28"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="16">
        <location>
            <begin position="41"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="14">
        <location>
            <begin position="143"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="15">
        <location>
            <begin position="406"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="17">
        <location>
            <begin position="698"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="212"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="220"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQJ90410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="805" mass="82798" checksum="DF8DB0D7FD54B56D" modified="2012-06-13" version="1">MPPMQQQPPPVVVLLLLFFLACSYTLASGSTDDEGAAAAATYIVYLNPALKPSPYATHLHWHHAHLESLSLDPSRSLLYSYTTAAPSAFAARLLPSHATELQSHPAVASVHEDVLLPLHTTRSPLFLHLPPYDDPAAADAGGGADVIIGVLDTGVWPDSPSFVDTGLGPVPARWRGSCDTKAADFPSSLCNRKLIGARAFFRGSSASAGAAAAAGGGRNGSSSSSHGVNGEVSASPRDRDGHGTHTASTAAGAVVAGASLLGYARGTARGMAPGARVAAYKVCWRQGCFSSDILAGMEQAIDDGVDVLSLSLGGGALPLSRDPIAVGALAAARRGIVVACSAGNSGPSPSSLVNTAPWVITVGAGTLDRNFPAYAKLGNGETHAGMSLYSPGEDDEDDDDGDKMFPLVYDKGFRTGSKLCMPGSLDAAAVKGKVVLCDRGGNSRVEKGQVVKQAGGVGMVLANTAQSGEEIVADSHLLPAVAVGAKSGDAIRRYVESNDDAEVALSFGGTAVDVHPAPVVAAFSSRGPNRVVPQLLKPDVIGPGVNILAGWTGSVGPTGLIADERRPKFNILSGTSMSCPHISGLAAFVKAAHPDWSPSAIKSALMTTAYAVDNNGSPLLDAAGDNTTATPWSFGSGHVDPVKALSPGLVYDTSIDDYVAFLCTVGGASSPRQIQAITGSRTAKGNATCQRKLSSPGDLNYPSFSVVYPLRKSHSTVKYRRELTNVGAAGSVYTVKVTGGPSSVSVAVKPARLVFKKAGDKLKYTVAFKSSAQGAPTDAAFGWLTWSSADGEHDVRSPISYTWGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1IRW6</accession>
    <name>I1IRW6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100826693</name>
        <name evidence="8" type="ORF">BRADI_4g35342v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the detoxification of the Fusarium mycotoxin deoxynivalenol by the transfer of glucose from UDP-D-glucose to the hydroxyl group at C-3, forming deoxynivalenol-3-O-beta-D-glucoside.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576736.1">
        <property type="nucleotide sequence ID" value="XM_003576688.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IRW6"/>
    <dbReference type="EnsemblPlants" id="KQJ91064">
        <property type="protein sequence ID" value="KQJ91064"/>
        <property type="gene ID" value="BRADI_4g35342v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826693"/>
    <dbReference type="Gramene" id="KQJ91064">
        <property type="protein sequence ID" value="KQJ91064"/>
        <property type="gene ID" value="BRADI_4g35342v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826693"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001724_0_1_1"/>
    <dbReference type="OMA" id="STWRAKM"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098754">
        <property type="term" value="P:detoxification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000057">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1403">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0216">Detoxification</keyword>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00058521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQJ91064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="464" mass="49956" checksum="7B9ECF7D460620D7" modified="2012-06-13" version="1">MVRADESIHVLLVPYPAQGHLNPILQFGKRLAGHGGAVRCTVAVTRFVLGSTKPAPIGSVHVGVISDGCDALGPAELGGHQGPYFERLEAAGSETLDGLLRSEAAQGRPVRVVVYDPFMPWVQDLARRHGAACAAFLTQTCAVDIVYTHARAGRLPVPVRRDDGAAGLLELPGLSARLSAADVPTFLTDTDAHHPSMRDLLMNQFVGLRTVDHVLVNSFFDLEPQEAEHLASTLGAKTIGPTVPSAYLDKRLPADVSYGFHLHTPMTSECKAWLDAHRARSVVYASFGSIVAPGAEQMGEVAEGLQSTGAPFLWVVRATEASKLPEGFASEAKAHGHLIVPWCPQLEVLAHEAVGCFVTHCGWNSTVEALSAGVPMVAVPQWSDQPTNAKYIQDVWRVGVRVRQDGEGVVRKEEVERCVKEVMDGEGYRKRAAAWRAKANKAMSQGGSSDRNIAEFLSKYRAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1IT64</accession>
    <name>I1IT64_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100834663</name>
        <name evidence="3" type="ORF">BRADI_4g38920v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Bd21</strain>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578701.1">
        <property type="nucleotide sequence ID" value="XM_003578653.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IT64"/>
    <dbReference type="EnsemblPlants" id="KQJ91649">
        <property type="protein sequence ID" value="KQJ91649"/>
        <property type="gene ID" value="BRADI_4g38920v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834663"/>
    <dbReference type="Gramene" id="KQJ91649">
        <property type="protein sequence ID" value="KQJ91649"/>
        <property type="gene ID" value="BRADI_4g38920v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100834663"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_21_1_1"/>
    <dbReference type="OMA" id="RRWIDKR"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46863">
        <property type="entry name" value="OS09G0572100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46863:SF6">
        <property type="entry name" value="OS09G0572100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="86"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ91649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="394" mass="41000" checksum="E7C459C0B5401C3D" modified="2012-06-13" version="1">MCKKSSSTDALPSAASRRSSAANLTSSSSARTASSTFSATGPRNTAAAANISSSSTTAAASSSSRSSGRSSLAAARASLPDPPVLYSFQELAAATNSFLAKRASDSSDSAYPYWRCVLRARHAALFQLRSSPGLTKAAASAALARVARYHHTGLAPLLGACLAGPHVYLAYELPDPSAATLAACLQPRKNRNNAAGFTVLRTWLSRVQIAADVAQGLDYVHTHAAQIHGRVSPSAVLVSDPGLRARLTHFGASQFAAAESDSSADAPSKEADVYAFGVLLLGLLSGEAAAARHRFDGATGEFVRVTVVETAAEAARSGRVRGWVDRRLGDSFPVAAAERLVEVGIRCAAAEAADRPDMSWAAGKVSKAYVESIAWEHSLRPPTNSEFSVSLAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>I1IUS8</accession>
    <name>I1IUS8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Histone-lysine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100825241</name>
        <name evidence="14" type="ORF">BRADI_4g43717v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Bd21</strain>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577097.1">
        <property type="nucleotide sequence ID" value="XM_003577049.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024319308.1">
        <property type="nucleotide sequence ID" value="XM_024463540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IUS8"/>
    <dbReference type="STRING" id="15368.I1IUS8"/>
    <dbReference type="EnsemblPlants" id="KQJ92446">
        <property type="protein sequence ID" value="KQJ92446"/>
        <property type="gene ID" value="BRADI_4g43717v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825241"/>
    <dbReference type="Gramene" id="KQJ92446">
        <property type="protein sequence ID" value="KQJ92446"/>
        <property type="gene ID" value="BRADI_4g43717v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825241"/>
    <dbReference type="eggNOG" id="KOG1082">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004556_2_1_1"/>
    <dbReference type="OMA" id="VEEIMMM"/>
    <dbReference type="OrthoDB" id="5792673at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.280.10:FF:000003">
        <property type="entry name" value="Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.280.10">
        <property type="entry name" value="SRA-YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025794">
        <property type="entry name" value="H3-K9-MeTrfase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051357">
        <property type="entry name" value="H3K9_HMTase_SUVAR3-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036987">
        <property type="entry name" value="SRA-YDG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003105">
        <property type="entry name" value="SRA_YDG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660:SF1">
        <property type="entry name" value="OS11G0131600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02182">
        <property type="entry name" value="SAD_SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00466">
        <property type="entry name" value="SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51575">
        <property type="entry name" value="SAM_MT43_SUVAR39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51015">
        <property type="entry name" value="YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YDG" evidence="13">
        <location>
            <begin position="215"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-SET" evidence="11">
        <location>
            <begin position="436"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="10">
        <location>
            <begin position="500"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="12">
        <location>
            <begin position="661"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ92446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="75443" checksum="FEAB8D75CD752AFE" modified="2012-06-13" version="1">MESFKDGAETTEQPLDMKPLLSLAPMFPAPAGYDIATQSGDPPLVYVTPIFRSCPSSERPMPSSEQPRPAEPASFARPSPVSSVPLKAIPISAAFRKPRRKDESSDEDYDPFCKKRKTSTLKRTTKKVQLAGDSNAVNVKQRPIRRSLSKELAGWPSSADSPRESVEATMIMFDSLRRRMLQLDEKEDAGKRADLKAGALMMQNNLRINSLKKMGPVPGVEIGDIFFFRFEMCTVGLHAQSMAGIDYMSSKHAGKDVSLAVSIISSGGYENAEDDTDTLVYTGQGGNSRYKEKHDQKPERGNLALMNSTKRKNQIRVVRGVQDPFCNSGKIYIYDGLYRIEDSWMDMAKNGFNVFKYKLRREPGQPDGISVWKMTEKWKANPVTRGRFITLDLSSEIENQPVCVVNDVDNEKGPSHFEYVTGVKYLRPLSRKKPLQNCKCPSVCLPGDPNCSCMKQNGGDLPYSSSGVLVKHVPILYECSSDCHCSQDCRNRVAQKGVKLNFEVFWTGDRGWGLRSWDPIRAGTFVCEYAGEVIDETNMNTDVEEHEYTFRTSCPGDKVLRWNLGAELLEEKSTDATAESFRQLPIIMSAKDAGNVARFLNHSCSPNLLWQAVQYDHGDDSYPHIMFFAMKHIPPMTELTYDYGARGAPPGIKGKFPQACKLNACLCGSTNCRGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVT4</accession>
    <name>I1IVT4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100842404</name>
        <name evidence="3" type="ORF">BRADI_5g01880v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580554.1">
        <property type="nucleotide sequence ID" value="XM_003580506.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVT4"/>
    <dbReference type="STRING" id="15368.I1IVT4"/>
    <dbReference type="EnsemblPlants" id="KQJ81629">
        <property type="protein sequence ID" value="KQJ81629"/>
        <property type="gene ID" value="BRADI_5g01880v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842404"/>
    <dbReference type="Gramene" id="KQJ81629">
        <property type="protein sequence ID" value="KQJ81629"/>
        <property type="gene ID" value="BRADI_5g01880v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842404"/>
    <dbReference type="eggNOG" id="KOG2184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051088_0_0_1"/>
    <dbReference type="OMA" id="RWTNGRQ"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IVT4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47251">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04180)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47251:SF1">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04180)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="56"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ81629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="294" mass="34064" checksum="59CE808887F53A25" modified="2012-06-13" version="1">MEKRDQHSKDPPGKEAEGEDDEYDLEEFRLPMSHRPTENLDTEGLEQASVDTQLSSSNLGFRLLQKMGWKGKGLGKNEQGILEPIKAGIRDAKLGVGKQEQDDFFTSEENVQRKRLNIELEETEEHIKKREVTAEREQKIRSEVKEIQKVFFCNLCNKQYKLAHEFESHLSSYDHNHRKRFKEMREMQSSSNGNRDDRQKREQQREEKELAKFAQLADAHRKQQQQKQEQPEISGEQVMTKNLPTPGNQDQRRTLKFGFSKMAPSKAPVGHISKKPKIASKVPSVFGNDSDEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IWE7</accession>
    <name>I1IWE7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Glycerol-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="5 11">1.1.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100837232</name>
        <name evidence="15" type="ORF">BRADI_5g03810v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a quinone + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:18977"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="5 11"/>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003581351.1">
        <property type="nucleotide sequence ID" value="XM_003581303.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IWE7"/>
    <dbReference type="STRING" id="15368.I1IWE7"/>
    <dbReference type="EnsemblPlants" id="KQJ81920">
        <property type="protein sequence ID" value="KQJ81920"/>
        <property type="gene ID" value="BRADI_5g03810v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837232"/>
    <dbReference type="Gramene" id="KQJ81920">
        <property type="protein sequence ID" value="KQJ81920"/>
        <property type="gene ID" value="BRADI_5g03810v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837232"/>
    <dbReference type="eggNOG" id="KOG0042">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015740_4_3_1"/>
    <dbReference type="OMA" id="PHIVKPM"/>
    <dbReference type="OrthoDB" id="264015at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IWE7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006127">
        <property type="term" value="P:glycerol-3-phosphate shuttle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000001">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.870">
        <property type="entry name" value="Alpha-glycerophosphate oxidase, cap domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031656">
        <property type="entry name" value="DAO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038299">
        <property type="entry name" value="DAO_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985:SF15">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16901">
        <property type="entry name" value="DAO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00977">
        <property type="entry name" value="FAD_G3PDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00978">
        <property type="entry name" value="FAD_G3PDH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="6 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095568" description="Glycerol-3-phosphate dehydrogenase" evidence="12">
        <location>
            <begin position="35"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="13">
        <location>
            <begin position="76"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-glycerophosphate oxidase C-terminal" evidence="14">
        <location>
            <begin position="466"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQJ81920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="632" mass="68411" checksum="BCC72097FCDAF9F5" modified="2012-06-13" version="1">MAAWRLRRAGTALAASSALAAAAAAASAWSSASASDSTPVALGAARQRVTQPGAAAPPRAAQLAALAGSTAAEPLDVLVVGGGATGCGVALDAATRGLRVGLVEREDFSSGTSSRSTKLIHGGVRYLEKAVFNLDYGQLKLVFHALKERKQVIENAPHLCHALPCMTPCFNWFEVVYYWFGLKFYDIVAGRRLLHLSRYYSVEESVELFPTLARNDGDRSLRGTVVYYDGQMNDSRLNVGLACTSAVVGAAILNYAEVISLIKDESGERIIGAHIRDALSGKEFDTFAKVVVNASGAFCDNVRKMANSDVVPMICPSSGVHIVLPDYYSPEGMGLIVPKTKDGRVVFMLPWLGRTIAGTTDSSTAITMLPEPHEDEIQFILDAICDYLNVQVRRSDVLSAWSGIRPLAMDPSAKNTESISRDHVVFEDYPGLITITGGKWTTYRSMAEDAVNAAIRSGNLKPANDCVTDHLHIAGGYGWDPASFTVLAQNFKRMKKTYGGKVIPGAMDSAVSKHLSHAYGTLAEQVAAIAQNEGLGKRLAHGYPFLEAEVAYCARHEYCESAVDFVARRCRLAFLDTDAAGRALPRIIEILASEHKWDKARRKLELQKGIEFLETFKSSKNAQFRDGKHNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I1IXA1</accession>
    <name>I1IXA1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100822806</name>
        <name evidence="3" type="ORF">BRADI_5g08690v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579654.1">
        <property type="nucleotide sequence ID" value="XM_003579606.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IXA1"/>
    <dbReference type="FunCoup" id="I1IXA1">
        <property type="interactions" value="1159"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IXA1"/>
    <dbReference type="EnsemblPlants" id="KQJ82387">
        <property type="protein sequence ID" value="KQJ82387"/>
        <property type="gene ID" value="BRADI_5g08690v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT60967">
        <property type="protein sequence ID" value="PNT60967"/>
        <property type="gene ID" value="BRADI_5g08690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822806"/>
    <dbReference type="Gramene" id="KQJ82387">
        <property type="protein sequence ID" value="KQJ82387"/>
        <property type="gene ID" value="BRADI_5g08690v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60967">
        <property type="protein sequence ID" value="PNT60967"/>
        <property type="gene ID" value="BRADI_5g08690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822806"/>
    <dbReference type="eggNOG" id="ENOG502QQ6Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021999_1_0_1"/>
    <dbReference type="OMA" id="MEEMRNC"/>
    <dbReference type="OrthoDB" id="1925034at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IXA1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd07307">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037488">
        <property type="entry name" value="At2g33490-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34119">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34119:SF1">
        <property type="entry name" value="OS04G0394700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="348"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="539"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="562"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="615"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ82387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="69591" checksum="BD634A914019F213" modified="2012-06-13" version="1">MKSPLRKFRGLSLPHHHKERKDQRPPPAKLDELVDAAQEMVEMRNCYDSLLSAAAATTNSVYEFAEAMEEMGTCLLEKTALDYDDDDSGRVLMMLGKAQFELQKFVDSYRTNIINTITNPSESLLKELQVVEEMKDQCDQKREEYETVRAAYREKGRSKHSKIETFSTEQLQASFLEYQEDAALFIFRLKSLKQGQFLSILTQAARHHAAQLSFFRRGLKYLEALEPHVKAVAEKQHIDYHFSGLDDDTDNDDYSSYQDNHSDGSELSFDYGINFPASRSSMDLDQSNVASSPKHLKEHEQENAEQMKADFVAPRVKPEFGTQSAPIFAENMLDPSVRFQKMNSPTFLSPLHSSRTNYSYKLPTPADDKNTSAETNRSPHSDKPESKSSMAANLWHSSPLVKGFKPSSLYSGPVKMPSSTEGTSSPLVYSYSTSDSKKMKRESFSGPIPSKAGLSKPLFSAAGQRASMNYPRVMSAKSHEPGWQSSVAPKVPRITSLPTTSPRISELHELPRPPSVGTARPGLVGYSGPLVSRRQIPNVPTRASPPSHTASPLPRPPAAMTRSYSIPSNSQRTPIITVNKLLEARHSRESSEVSSPPLTPISLADVSRKSPVTTIDKRRIKETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I1J195</accession>
    <name>I1J195_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC104585415</name>
        <name evidence="1" type="ORF">BRADI_5g20197v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240349.1">
        <property type="nucleotide sequence ID" value="XM_010242047.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J195"/>
    <dbReference type="FunCoup" id="I1J195">
        <property type="interactions" value="1497"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1J195"/>
    <dbReference type="EnsemblPlants" id="KQJ84336">
        <property type="protein sequence ID" value="KQJ84336"/>
        <property type="gene ID" value="BRADI_5g20197v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104585415"/>
    <dbReference type="Gramene" id="KQJ84336">
        <property type="protein sequence ID" value="KQJ84336"/>
        <property type="gene ID" value="BRADI_5g20197v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104585415"/>
    <dbReference type="eggNOG" id="ENOG502QT0G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041607_0_0_1"/>
    <dbReference type="OMA" id="VENDISM"/>
    <dbReference type="OrthoDB" id="25131at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038925">
        <property type="entry name" value="At3g17800-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008479">
        <property type="entry name" value="DUF760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31808">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31808:SF2">
        <property type="entry name" value="OS04G0596300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05542">
        <property type="entry name" value="DUF760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ84336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="391" mass="43841" checksum="EB0CB6A65F1C31E9" modified="2012-06-13" version="1">MSAWAADAALLLASPSPASSRSLVRPRSSRLCKGFPCTSRSKAGFQISNYRTSILKVKAKMDSGDGETQLAPLKFETPSGQLLVQILQSHPHLIPVTVDQQLENLQSEKDAQNEEAAKVPQDLLYKRIAEVKEKERQTTLEEIIYCWILFKFVENDISMTPSLSSSSGPVRDISLPNQEYKLQSIHSQDALEMIQNHLNLIMGEQAAAPLDTVVEISNLNLGKLYAASIMYGYFLKRVDERFQLEKTMKTLPPSPKEQIVMERDLKPNPFWDMESLVQISPDGEEVDLDDEESNPNKLRSYVSRLDADTLQKYATIRSKESVSLIEKQTQALFGRPDIKVLDDGSVNAKDGKIVTITFTELTNLVLEAAGFGAFLWEAESHIESKYHFVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1J3T4</accession>
    <name>I1J3T4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100844532</name>
        <name evidence="20" type="ORF">BRADI_5g27480v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">Bd21</strain>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Endomembrane system</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Prevacuolar compartment membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Protein storage vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ85502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240672.1">
        <property type="nucleotide sequence ID" value="XM_010242370.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J3T4"/>
    <dbReference type="STRING" id="15368.I1J3T4"/>
    <dbReference type="EnsemblPlants" id="KQJ85502">
        <property type="protein sequence ID" value="KQJ85502"/>
        <property type="gene ID" value="BRADI_5g27480v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844532"/>
    <dbReference type="Gramene" id="KQJ85502">
        <property type="protein sequence ID" value="KQJ85502"/>
        <property type="gene ID" value="BRADI_5g27480v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035275_2_0_1"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1J3T4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032586">
        <property type="term" value="C:protein storage vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02123">
        <property type="entry name" value="PA_C_RZF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000276">
        <property type="entry name" value="Receptor homology region transmembrane domain-and RING domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000020">
        <property type="entry name" value="Receptor homology region transmembrane domain-and RING domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051653">
        <property type="entry name" value="E3_ligase_sorting_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044744">
        <property type="entry name" value="ZNRF4/RNF13/RNF167_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47168">
        <property type="entry name" value="RING ZINC FINGER DOMAIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47168:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="242"/>
            <end position="284"/>
        </location>
    </feature>
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        <location>
            <begin position="290"/>
            <end position="345"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="448"/>
            <end position="486"/>
        </location>
    </feature>
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        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
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    </evidence>
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        <source>
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    <evidence type="ECO:0000256" key="18">
        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
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        <source>
            <dbReference type="EMBL" id="KQJ85502.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ85502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="518" mass="55332" checksum="0AF5A2B32EC82F36" modified="2012-06-13" version="1">METNNFKSRRGAMLFPLCLAAMICFMAQIGAANVVLMGNNLTLSFDDIEANFAPGVKGSGVDGVVYTAEPLNACSALTNKAVKGPPSPFALVIRGGCTFDEKVKNVQDAGFKAAIVYDNENSGVLVSMAGSSSGIHIYAVFISKVSGEVLKKFSGRTDVEVWLIPTFENSAGSIMAISFISLLAMSAVLATCFFVRRHRIRRDRPRIPEAREFHGMSSQLVKALPSLIFTKVQEDNCTSSLCAICLEDYNVGEKLRVLPCRHKFHAACVDMWLTSWRSFCPVCKRDADAGTSAPPASETTPLLSSAVQLPSPSSSFRSSVAASPPRPISRRPSSQSISRVYSVSGTPNSPSIQRYYRTSSAMSISRSGADLANMSSPLLRGSHLGSTHSLVGSHLSPPINISYAPPHMYHSAHASPSTHVSSSYIFNSGHSSSGYYLGSSTQHRSYLRRCGESGPSLSTMAPQSPQQQSQLHHGGESETNLAGASSAQSFRQSYLRHCGDSDASLSAMASSGQSLPGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EC59</accession>
    <name>A0A0Q3EC59_BRADI</name>
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_5g17935v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ83972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EC59"/>
    <dbReference type="EnsemblPlants" id="KQJ83972">
        <property type="protein sequence ID" value="KQJ83972"/>
        <property type="gene ID" value="BRADI_5g17935v3"/>
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        <property type="protein sequence ID" value="KQJ83972"/>
        <property type="gene ID" value="BRADI_5g17935v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EC59"/>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053313">
        <property type="entry name" value="RGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34961:SF11">
        <property type="entry name" value="OS04G0554900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34961">
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="43"/>
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    </feature>
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        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="146" mass="15517" checksum="937555EE44025ADD" modified="2016-01-20" version="1">MPTFSSPLPPKTRQLKVTAATPTTVLITLVFLALFVRSCEPRHLLPHGKDSSSRSPPPAPCKADGSPINQKKNLSNAVDATGTLVDQVDAKVKKGVPMAMASSGAGVQTTIASRVSRRLSQRELQGEGTEFHLDYAGPITHPPSHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name type="common">Purple false brome</name>
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        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ88716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237780.2">
        <property type="nucleotide sequence ID" value="XM_010239478.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EQY1"/>
    <dbReference type="EnsemblPlants" id="KQJ88716">
        <property type="protein sequence ID" value="KQJ88716"/>
        <property type="gene ID" value="BRADI_4g20680v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104584570"/>
    <dbReference type="Gramene" id="KQJ88716">
        <property type="protein sequence ID" value="KQJ88716"/>
        <property type="gene ID" value="BRADI_4g20680v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104584570"/>
    <dbReference type="OrthoDB" id="688001at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EQY1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
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        <property type="entry name" value="PIR2-like_helical"/>
    </dbReference>
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        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33120:SF53">
        <property type="entry name" value="OS03G0697833 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12274">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="35"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="PIR2-like helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="415"/>
        </location>
    </feature>
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        <location>
            <begin position="527"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="738" mass="83846" checksum="5A9E5E2453580F28" modified="2016-01-20" version="1">MAGERRPRHMMIGDLDHLDETPDGVERNISVVLACIHDYYKKALDRLPAQLIPSLLDAGFCFGFLDPVANIIANTVFQFRDGESKKRKRKRSRAFATTNNKGRIESLQQRRQRDAAFSSIIAESSSSKHSILRPPLLNGSAVPNGNGSVASRSLRGLATFLTSYCRYLTVRDALRYLRLSKADLLVAVRLVELDHALTHSFTINDPTAQVALRCAAISAVHPMVATFLSRSFSLASHLDEVSNILPEKGRVIGRSTIKRLFELSSNCKPELVDPLKPMQHAISRMQPYRRTEPVPAGLEDSLMRVLLDKIHVLYLEAISCMPKHDICSRHHRGLLKAGHCYGPFDPVTNIILNTIWYDTAFPTKQEFDVKLICTKSLARIECFSLDGLVAFTCSVFPEFSEYKAMEYLLVNNARIDRVTMKAIRKGHAHSSESDTYDTAARAANHPCPTALVQFATTGMQQVGKTLRKKLKCKPMLSPSDVCTISEMLSQNFLPGESAKLPQTHVLEIISARCEEFEDRQSVIFGRVEAALHMYAQQERQDYELQVICDLNDDIPDDGRYDYMLHHTRFSHINIWAKRKGSQYADEVPTLFFIECSNTNEEKDMQGMRFLCHPILEPSKYAGRCFHCEQEGFRIIHPPSEKGYLGRCTEFEDMASGKNPVSNEHLVDYGNYQTFFADGSETNDSIYFDPILGNEDAITRDQEWKEKRDPRRKDLSWLEDQLRQDLALARKKYRVSLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ETL3</accession>
    <name>A0A0Q3ETL3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100841159</name>
        <name evidence="2" type="ORF">BRADI_4g34400v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90855.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576704.2">
        <property type="nucleotide sequence ID" value="XM_003576656.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ETL3"/>
    <dbReference type="FunCoup" id="A0A0Q3ETL3">
        <property type="interactions" value="470"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ90855">
        <property type="protein sequence ID" value="KQJ90855"/>
        <property type="gene ID" value="BRADI_4g34400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841159"/>
    <dbReference type="Gramene" id="KQJ90855">
        <property type="protein sequence ID" value="KQJ90855"/>
        <property type="gene ID" value="BRADI_4g34400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841159"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3ETL3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08267">
        <property type="entry name" value="MDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052733">
        <property type="entry name" value="Chloroplast_QOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44013:SF2">
        <property type="entry name" value="OS09G0502500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44013">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C16A3.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="55"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ90855.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="393" mass="40775" checksum="E663CA6D8A4CDF79" modified="2018-03-28" version="2">MQKHHGARALPKIVVAGSTLFRRAAAKFTAMPSSTSSSSSPSRTMRAVLYDKYGGGAEGLKHVEVPIPSPKKGELLLRMEAASINRVDWRFQQGKARPILPSKFPFTPVCELAGEVVELGSGVSGFAAGDKVIAVNFPGGGGLAEYAVVSASCAATRPPEVSATEGACLPIAAATALAALRTAGVGLDARGPPKNVLVTAASGGVGTFAVQLAKLSGKHHVTVTSGARNLGLVRALGANDALDYGTPEGAALRRAGPAGRKHDAVVHCAEGFPWSAFEPALADAGGVVVDLTARVASVAVAALQRVLFARKRLVPLLVSPKKEDMEVLLGLVRRGQIRVVIDSRYPLSRAHEGWAKSMGGHATGKIVVEMGVTEGARTRRTSSSHLFVPLNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3EZ58</accession>
    <name>A0A0Q3EZ58_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100821153</name>
        <name evidence="14" type="ORF">BRADI_4g39617v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578742.1">
        <property type="nucleotide sequence ID" value="XM_003578694.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3EZ58"/>
    <dbReference type="FunCoup" id="A0A0Q3EZ58">
        <property type="interactions" value="1173"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ91772">
        <property type="protein sequence ID" value="KQJ91772"/>
        <property type="gene ID" value="BRADI_4g39617v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821153"/>
    <dbReference type="Gramene" id="KQJ91772">
        <property type="protein sequence ID" value="KQJ91772"/>
        <property type="gene ID" value="BRADI_4g39617v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821153"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3EZ58">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000275">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000722">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF36">
        <property type="entry name" value="RECEPTOR PROTEIN KINASE-LIKE PROTEIN ZAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129097" description="Protein kinase domain-containing protein" evidence="12">
        <location>
            <begin position="26"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="445"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="394"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="606"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="671"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="401"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="679"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ91772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="784" mass="82013" checksum="A4BC1AB7FA1EA41C" modified="2016-01-20" version="1">MRTIPATILLILLLLIALPPPPPSAMALTADGLALLAFKAAVTEDPTSALSSWSESDADPCRWSGVTCANISSAQPQQPPQPRVVGLAVAGKNVSGYIPSELGSLLFLRRLNLHDNRLTGAIPAALSNASSLHSLFLYNNALTGVLPVAALCSGLPRLRNLDISSNALSGELPLELRGCRGLQRLVLSGNRFSGEVPGGIWPEMAPSLQQLDISSNTFNGSVPPSLGMLGKLFGTLNLSHNEFSGVVPPELGRLPAAVALDLRFNNLSGAIPQMGSLASQGPTAFLNNPALCGFPLQVACRAVPPPTLSPAPPQNTSPSTAAAAADQGRQHHPIKTNLIALISVADAAGVALVGVILVYIYWKVKDRKKSRDHDDEDDEDRKQGLCRCMWARRGRGGSRDESDDGGSSDDDEEEAGVRKQGGGGGDGELVAIDKGFRVELDELLRSSAYVLGKGGKGIVYKVVVGGGSTPVAVRRLGGGVGGADRRKEFRAEARAMGRVRHPNVVRLRAFYWSPDEKLVVTDFVGNGNLATALRGRSGEPALSWAARLKIAKGAARGLAHLHECSPRRFVHGEVKPSNILLDADFTPRVADFGLVRLLAIAGCAQDTSLPQPPPPSSGGLLGGAIPYTKPAPGQGGAGYRAPEARTAGARPAQKWDVFSFGVVLLELLTGRGPASDHASSPSTSASFSGPASSSTGTTTDRSGSGEHGGGAGVPEVVRWVRRGFEEDSRPVAEMVDPALLRAAPTLPKKELVAAFHAALACTEADPELRPKMKTVAESLDKIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F2M8</accession>
    <name>A0A0Q3F2M8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100825309</name>
        <name evidence="10" type="ORF">BRADI_3g06150v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ93686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570779.2">
        <property type="nucleotide sequence ID" value="XM_003570731.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F2M8"/>
    <dbReference type="STRING" id="15368.A0A0Q3F2M8"/>
    <dbReference type="EnsemblPlants" id="KQJ93686">
        <property type="protein sequence ID" value="KQJ93686"/>
        <property type="gene ID" value="BRADI_3g06150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825309"/>
    <dbReference type="Gramene" id="KQJ93686">
        <property type="protein sequence ID" value="KQJ93686"/>
        <property type="gene ID" value="BRADI_3g06150v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825309"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3F2M8">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000223">
        <property type="entry name" value="probable receptor-like protein kinase At1g80640"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000342">
        <property type="entry name" value="Protein kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF27">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043129099" description="Protein kinase domain-containing protein" evidence="8">
        <location>
            <begin position="37"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="180"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ93686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ93686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="478" mass="51689" checksum="7C10BF6278319C7D" modified="2016-01-20" version="1">MPAGMWSMQPPAPLPLLAFSVFFLLPLLCFPLAANGGSPPPTASRSFFPVVPAATTTVGVDAFSSNGSAAAAAATPAAGPSAPFVLTVEGGRHPLRKELIIAIVLASIAGVTIVVAAIYAFIVWWRYRRSLDDFKDTQSTDTARVALVPILNKFNSFKASKKDLVAMMEYASLEAATGNFSESNVLGIGGYGCVYKANFDGGCVAAVKRLGREGQECGKEFENELDLLQRIQHLNIVSLVGFCIHEENRFIVYELMVNGSLETQLHGPSHGSALSWHIRMKIALDTARGLEYLHEHCNPPVIHRDLKSSNILLDSDFNAKISDFGLAVASGNHSKGNLKLSGTLGYVAPEYLLDGKLTEKSDVYAFGVVLLELLLGRRPVEKMAPSQCQSIVTWAMPQLIDRAKLPTIIDSVIRNTMDLKHLYQVAAVAVLCVQPEPSYRPLITDVLHSLTPLVPLELGGTLRINPESPCAMHNRSPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FAJ2</accession>
    <name>A0A0Q3FAJ2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC104583620</name>
        <name evidence="5" type="ORF">BRADI_3g22570v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234647.1">
        <property type="nucleotide sequence ID" value="XM_010236345.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FAJ2"/>
    <dbReference type="STRING" id="15368.A0A0Q3FAJ2"/>
    <dbReference type="EnsemblPlants" id="KQJ96342">
        <property type="protein sequence ID" value="KQJ96342"/>
        <property type="gene ID" value="BRADI_3g22570v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104583620"/>
    <dbReference type="Gramene" id="KQJ96342">
        <property type="protein sequence ID" value="KQJ96342"/>
        <property type="gene ID" value="BRADI_3g22570v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104583620"/>
    <dbReference type="OrthoDB" id="3800936at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FAJ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="237"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="318"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="414"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="500"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="551"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="631"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="40"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ96342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="741" mass="79953" checksum="9BBD401CF671A5A8" modified="2016-01-20" version="1">MPRARKRRAPAAAASECSPVQGVTPGKTAEAGVRVRDEGEAMEVEPEEANGLESDQEDPDEVEPEEQDPDVDDPEEAEEDPEEAAGSDEGDGNEEGADEDQGNGEEVVGLGDDSAGVESEKAGIKIGGDEEKGTPIVLKEGGDEDTCMEPAVVEKPEEAVKEDAMENDTANLSGDGATTDDMNEIPGEEHEESGDEGTKDKDGIAGQLSVNGDVRTVELDPSLPALGPVSVENAKDLELFVGGLPKDCAEEDITVVFSQFGEIESIKIIKHSARKKGNCIAFVRYMNTEAAKKAVAEFKEGVEVKGKTVTVSAAQGNNTLYLGNICKSWTKDKVLNTLKSIGVEELEMSLPDDPDTGGRNRGFAFLKFSALDKTEAAFQQLLKQRDAFIDIDRSANFSARTPTESSEELAMKIKTVYLEHVPLSWDKSKIEECCEAYGEIHEVRLVKKSRKKISFVEFSSRMSALACVEGINSAKIGGEGGEVKLAASLARPRRLKLDDSAKGGLKVNSGATSEDANSSIKKKDQKKEVVVKSSNKLLKRDVDKLVSQVDAEVAQTSSRYKGKKKSGKNESTSVNERPSKKARKNRDVPTRSSNRTRHGGSSRAAYGGKSAGNMKLSAGARYATSYQSYPAAGASSRSKPNSRDLEPHAGYIPPANRIPANHVQITYVYDQPRSAPSTFCHIDSVPHAREIGALQPTYSIYTSNPQYQGGYAYTYLPPPPSGSYRLGSGAYSPPRRYTDYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FDF6</accession>
    <name>A0A0Q3FDF6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pirin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100825215</name>
        <name evidence="5" type="ORF">BRADI_3g21730v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571689.1">
        <property type="nucleotide sequence ID" value="XM_003571641.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FDF6"/>
    <dbReference type="FunCoup" id="A0A0Q3FDF6">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FDF6"/>
    <dbReference type="EnsemblPlants" id="KQJ96236">
        <property type="protein sequence ID" value="KQJ96236"/>
        <property type="gene ID" value="BRADI_3g21730v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825215"/>
    <dbReference type="Gramene" id="KQJ96236">
        <property type="protein sequence ID" value="KQJ96236"/>
        <property type="gene ID" value="BRADI_3g21730v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825215"/>
    <dbReference type="OrthoDB" id="198735at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FDF6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF11">
        <property type="entry name" value="OS08G0364900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="3">
        <location>
            <begin position="102"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="4">
        <location>
            <begin position="251"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ96236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="40109" checksum="E32C3A676126E512" modified="2016-01-20" version="1">MSAAACVALCPTASSRHGGSPAVVGTPPAAMAAEKEAEQKHLFSGSTEINANGSINGKCDEDGDAAAGVDDVAEHYRDPAVTKPRAVVQRFTCERKPFVDGFALRRSIGSPELESLDPFLSLDEFEFSPPAGFSDHPHRGFENVTYMLEGGLSYHDFSGHKGTINKGDVQWMTAGRGVVHAEMPGGQGVQRGINLWINLSSKDKMVAPRYQDLASVEIPTAEKDGVTIKVIAGSALGARSPLETRTPAMFLDVAMRPGARLRQPVPPGWTACAYVIDGEAGFFFGSGSGSQDAEAGAHECVVFGGDGDGDGVDVRSEGGGRILLLAARPHGEAVVRDGPFVMNTREEVEQAREDYLNRRNGFEMAAGWASAHAPTAVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FQB4</accession>
    <name>A0A0Q3FQB4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">adenosylmethionine decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BRADI_3g48490v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-adenosyl-L-methionine + H(+) = S-adenosyl 3-(methylsulfanyl)propylamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic AdoMetDC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="4"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FQB4"/>
    <dbReference type="FunCoup" id="A0A0Q3FQB4">
        <property type="interactions" value="1890"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FQB4"/>
    <dbReference type="EnsemblPlants" id="KQK00293">
        <property type="protein sequence ID" value="KQK00293"/>
        <property type="gene ID" value="BRADI_3g48490v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK00293">
        <property type="protein sequence ID" value="KQK00293"/>
        <property type="gene ID" value="BRADI_3g48490v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FQB4"/>
    <dbReference type="OrthoDB" id="1068353at2759"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FQB4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006597">
        <property type="term" value="P:spermine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.50:FF:000001">
        <property type="entry name" value="S-adenosylmethionine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.90.10:FF:000002">
        <property type="entry name" value="S-adenosylmethionine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.50">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.90.10">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048283">
        <property type="entry name" value="AdoMetDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001985">
        <property type="entry name" value="S-AdoMet_decarboxylase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018166">
        <property type="entry name" value="S-AdoMet_deCO2ase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00535">
        <property type="entry name" value="SAM_DCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570:SF3">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01536">
        <property type="entry name" value="SAM_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01336">
        <property type="entry name" value="ADOMETDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0704">Schiff base</keyword>
    <keyword evidence="7" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQK00293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="410" mass="44850" checksum="4F5EC4FAFC3A5175" modified="2016-01-20" version="1">MYEAPLGYCIEDLRPAGGIEKFRSAAYSNGFEKRLEITFSEAPVFSDPNGRGLRALSRAQIDSVLDLAKCTIVSELSNEDFDSYVLSESSLFVYPYKVVIKTCGTTKLLLAIPRILELAEELSLPLSAVKYSRGTFIFPEAQPAPHKNFADEVAFLNGYFGNLKSGGNAYVIGDPAKPGQNWHVYYATQQPEHPVVNLEMCMTGLDKKKASVFFKTSADGHTSCAKEMTKLSGICDIIPEMEICDFDFEPCGYSMNATHGPAFSTIHVTPEDGFSYASYEVMGLDPASISYGNLVKRVLSCFGPSEFSVAVTVFGGRGHAETWAEKLVIGAYDSTNMVEQELPAGGLLIYQSFTSIGEVPTGSPRSVLSCFADEHVESGPKVGKMDAYLCWEEDHVDDTDVRDGKKMRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GGL2</accession>
    <name>A0A0Q3GGL2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g48255v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Poa p IX/Phl p VI allergen family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GGL2"/>
    <dbReference type="EnsemblPlants" id="KQK09485">
        <property type="protein sequence ID" value="KQK09485"/>
        <property type="gene ID" value="BRADI_2g48255v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK09485">
        <property type="protein sequence ID" value="KQK09485"/>
        <property type="gene ID" value="BRADI_2g48255v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GGL2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.320">
        <property type="entry name" value="Group V grass pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035506">
        <property type="entry name" value="Pollen_allergen/Os"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00833">
        <property type="entry name" value="POAALLERGEN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81736">
        <property type="entry name" value="Group V grass pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK09485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="67" mass="6961" checksum="83F66C336C12FDF0" modified="2016-01-20" version="1">MAGMSIDGKAYTDYKFIPPLDAAFKEAYASTVAAKPEVKYAVFEAALTKAIGAMAEAEKAASTKPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GMX2</accession>
    <name>A0A0Q3GMX2_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_5g06127v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GMX2"/>
    <dbReference type="EnsemblPlants" id="KQJ82114">
        <property type="protein sequence ID" value="KQJ82114"/>
        <property type="gene ID" value="BRADI_5g06127v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82114">
        <property type="protein sequence ID" value="KQJ82114"/>
        <property type="gene ID" value="BRADI_5g06127v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GMX2"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ82114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="81" mass="8484" checksum="DE0B6B5935273AB9" modified="2016-01-20" version="1">MAAAGTAFRPVLSHSAPSAQLPPVLLQTLIDGVVELVECLRLADIGGDRASLVTGLLVLVQHELIIVLVWSCAGCCTVLFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GNX3</accession>
    <name>A0A0Q3GNX3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC104581931</name>
        <name evidence="8" type="ORF">BRADI_1g01801v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010229408.1">
        <property type="nucleotide sequence ID" value="XM_010231106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GNX3"/>
    <dbReference type="STRING" id="15368.A0A0Q3GNX3"/>
    <dbReference type="EnsemblPlants" id="KQK12133">
        <property type="protein sequence ID" value="KQK12133"/>
        <property type="gene ID" value="BRADI_1g01801v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104581931"/>
    <dbReference type="Gramene" id="KQK12133">
        <property type="protein sequence ID" value="KQK12133"/>
        <property type="gene ID" value="BRADI_1g01801v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104581931"/>
    <dbReference type="OrthoDB" id="675031at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF179">
        <property type="entry name" value="OS08G0148400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="211"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK12133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="259" mass="28576" checksum="5C0654DD9676B321" modified="2016-01-20" version="1">MAAGVVFTPSDHDLVVHFLRPRIAGADVFGRGRFFHDADVYSAAPADLVHGRDHAPGTNKLDSKGMDRSTWYFFSPAQRLQTKSGRVGGRRQGGEKPVLDSDGRRVGYMRKLSFLIKKTMPGEPMTRVGWCMTEYEIDGDEDRLVLCKVYRSRSRSKNTETASMVAGSKKRKAADDDELYALGVYAGAADAANQHKNVCRRQEMPPRIQEIETMGGAEQVRNPSERAEEDDGRAGGGRGCDEDDGRFECKLEELLGGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GQR5</accession>
    <name>A0A0Q3GQR5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome f</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BRADI_1g05781v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="17">Binds 1 heme group covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast thylakoid membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome f family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GQR5"/>
    <dbReference type="STRING" id="15368.A0A0Q3GQR5"/>
    <dbReference type="EnsemblPlants" id="KQK12757">
        <property type="protein sequence ID" value="KQK12757"/>
        <property type="gene ID" value="BRADI_1g05781v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12757">
        <property type="protein sequence ID" value="KQK12757"/>
        <property type="gene ID" value="BRADI_1g05781v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GQR5"/>
    <dbReference type="OrthoDB" id="604292at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.700:FF:000001">
        <property type="entry name" value="Cytochrome f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.830:FF:000001">
        <property type="entry name" value="Cytochrome f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.830">
        <property type="entry name" value="Cytochrome f large domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.700">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024058">
        <property type="entry name" value="Cyt-f_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002325">
        <property type="entry name" value="Cyt_f"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024094">
        <property type="entry name" value="Cyt_f_lg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036826">
        <property type="entry name" value="Cyt_f_lg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33288">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33288:SF10">
        <property type="entry name" value="CYTOCHROME F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01333">
        <property type="entry name" value="Apocytochr_F_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16639">
        <property type="entry name" value="Apocytochr_F_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00610">
        <property type="entry name" value="CYTOCHROMEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103431">
        <property type="entry name" value="Cytochrome f subunit of the cytochrome b6f complex, transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49441">
        <property type="entry name" value="Cytochrome f, large domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51010">
        <property type="entry name" value="CYTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 17" id="KW-0349">Heme</keyword>
    <keyword evidence="13 17" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome f large" evidence="18">
        <location>
            <begin position="36"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="17">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="17">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR602325-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQK12757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="290" mass="32169" checksum="A34631CD5E40EF77" modified="2016-01-20" version="1">MENRNTFSWVKEQMTQSIFVSIMIYVITRTSISNAYPIFVQQGYENPREATGRIVCANCHLASKPVDIEVPQAVLPDTVFEAVLRIPYDMQLKQVLANGKKGGLNVGAVLILPEGFELAPPDRISPDYRPDKKNILVIGPVPGKKYSEIVFPILSPDPATKKDAHFLKYPIYVRGNRGRGQIYPDGSKSNNTVYNATSTVDASDGRQLIDIIPPGPELLVSEGESIKLDQPLTSNPNVGGFGQGDAEIVLQDPLRVQGLLFFFASVILAQVFLVLKKKQFEKVQLYEMNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GRD2</accession>
    <name>A0A0Q3GRD2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA 4-demethylwyosine synthase (AdoMet-dependent)</fullName>
            <ecNumber evidence="6">4.1.3.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">BRADI_1g07110v3</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Endomembrane system</location>
            <topology evidence="17">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TYW1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="6"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A0Q3GRD2">
        <property type="interactions" value="1810"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3GRD2"/>
    <dbReference type="EnsemblPlants" id="KQK12965">
        <property type="protein sequence ID" value="KQK12965"/>
        <property type="gene ID" value="BRADI_1g07110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12965">
        <property type="protein sequence ID" value="KQK12965"/>
        <property type="gene ID" value="BRADI_1g07110v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3GRD2"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GRD2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000258">
        <property type="entry name" value="Probable methyltransferase PMT28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000196">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="16" id="KW-0456">Lyase</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="21">
        <location>
            <begin position="704"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="22">
        <location>
            <begin position="977"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="68"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="859"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="184"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="859"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KQK12965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1306" mass="145611" checksum="18E7E1165C32B26D" modified="2016-01-20" version="1">MTVFRGPPGRPSALVAWWRRRPPLGFAAKVSIAIALGLSFVIVWTTLSPTSASQQISTERTYFAAEIAEPPTASRNRTSGSHGKPHATRSSRGHKKRHSPTRSHSHRPNATPSPDATASKTNLTEPALRPDQEPNEKGPEPVTETETEPEEEQEQEVELSVPEDNGENTGKAPKEEDEKAPELDLVDESSELDGDEEDPEAAKRNPSKEKKKLPHLFSPAAHYHWKQCSAKSGHHYIPCVDFDADGSQRHHERSCPRSPVTCLVSLPKEYKPPVPWPERKEKVWYENIGHPRLASYAKGHSWLNRTGEHLVFPPEESEFKGGASHYIESIDEMAPDIDWGKNIRVALDIGCKSAGFGVALLEKDVITLSLGLANEQTDLAQVALERGIPATVGSLGSRRLPFPSGAFDVIHCSECNIAWHSNGGKLLLEMNRILRPGGYFIISSRHGDLESEKGISASMTALCWNAVAYNSDDVSELGVKIFQRPASNEEYDLRARKDPPFCKEDQNKATAWYIPIKHCLHKAPADIEERGSEWPEEWPKRLETFPDWLGDMQTRVAADHNHWKAVVEKSYLDGLGIDWSNTRNVLDMKAVYGGFAAALSSKKVWVMNVVPVHAPDTLPVIYERGLIGVYHDWCEPFSTYPRSYDLLHADHLFSRLKNRCKQPIVILVEMDRILRPGGWAIIREKLDILDPLEAILRSLHWEIVMTFRKDKEGIMSTLAVRLSRRIADAGVDARPTDAAAFDPDDLPSVPLLLLVLPTHDAGAPPPAAAFLARWLAESAADFRAGALLLSRVRFAIFGVGSRAYGDTFNAAARSFSRLLRELGAVEVIPVVEGDVDDGDLEIVFEEWCGKVLRVANGEEVEDGANGESGGFDELELEGEESDYDDDDDDEEEEEVVNGEVDMEDLAGKATARRQNGKVAGLPNGAENGAREMVTPIIRTSLEKQGYKIVGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNTAIDEHRKMVKQMKGVPGVKPELLAEGLSPRHCALSLVGEPIMYPEINTLVDDLHRRHISTFLVTNAQFPEKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHDKDQRTVYRLTLVKGWNAEEIDAYANLLNLGQPDFIEIKGVTYCGSSATSKLTMENVPWHADVKEFSEVLASKSGGVYELACEHVHSCCVLLAKVDKFKINGKWHTWIDYDRFHELVTSGKPFKSCDYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGTAALKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H6I2</accession>
    <name>A0A0Q3H6I2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g43390v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H6I2"/>
    <dbReference type="EnsemblPlants" id="KQK18568">
        <property type="protein sequence ID" value="KQK18568"/>
        <property type="gene ID" value="BRADI_1g43390v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18568">
        <property type="protein sequence ID" value="KQK18568"/>
        <property type="gene ID" value="BRADI_1g43390v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H6I2"/>
    <dbReference type="OrthoDB" id="681537at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046533">
        <property type="entry name" value="DUF6598"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065:SF103">
        <property type="entry name" value="DUF6598 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33065">
        <property type="entry name" value="OS07G0486400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20241">
        <property type="entry name" value="DUF6598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6598" evidence="2">
        <location>
            <begin position="95"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK18568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="336" mass="38083" checksum="9CE41A8A2F50B4DB" modified="2016-01-20" version="1">MCLSGPPNRKTGRRSDDDPAAAWLNTPCGKKTREGLLKEIASSADKEPWERFRDFWVDVWGEGGYFGKFEDNTAIPPMRYTFSDPDYGYPMETLLFFSVKVVKNDESLHWPLDVYGFVAVRDVLDRKRNMIFCCERDNCQTINQQKNLLTGPTRAVAVSVDPSYFEVKLKVKGATETEDRDFSLFASTYRSGSMVRTFTSKLSTLEMTFQEIPYSVEATVSLKVIDGSWPDGFRGEFSARIDSVPDMKFRLLDCGDDKLPTDVDGKIQLTRRVVSVELRGFLRVPVLAHHVNGKQIKRREAIFQPKRCGISSNSKLKVGSCSMEITVSWSLFTSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HSS0</accession>
    <name>A0A0Q3HSS0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC112272461</name>
        <name evidence="22" type="ORF">BRADI_4g37220v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">Bd21</strain>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024319056.1">
        <property type="nucleotide sequence ID" value="XM_024463288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HSS0"/>
    <dbReference type="EnsemblPlants" id="KQJ91371">
        <property type="protein sequence ID" value="KQJ91371"/>
        <property type="gene ID" value="BRADI_4g37220v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112272461"/>
    <dbReference type="Gramene" id="KQJ91371">
        <property type="protein sequence ID" value="KQJ91371"/>
        <property type="gene ID" value="BRADI_4g37220v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="619931at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3HSS0">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF819">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044545970" description="Receptor-like serine/threonine-protein kinase" evidence="18">
        <location>
            <begin position="30"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="20">
        <location>
            <begin position="31"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="21">
        <location>
            <begin position="345"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="524"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="552"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KQJ91371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="842" mass="90368" checksum="39D69BAC7C721965" modified="2016-01-20" version="1">MARAAGRVVVVAALAVVCFFFLSPRPCAGAGDELVAGELLAPGKPLVSDGGAFALGFFSPSISTPDRQYLGIWYNSIPVNTVVWVANRETPITNGTSAPRLALDNDSSNLVLSDADGRAVWTTGMASGSPPAALAVLTNAGNLVLRSANGTALWQSFDHPADTFLPGMKVWLNHRTHEGGRLVSWSSPEDPSPGRFSYGMDPDTALQLLVWDGTRPHWRSPVWNGYTVQASYVSGTGTVVYTAIVDTEDEISNTFTVSPGAAPTRFVLTSSGQFQLLGWNGSASAWATVGSWPSSGCSRYGYCGPYGYCDVAAAACRCLDGFEPAWATGGDFSKGCRRKEPLPPCGHGSGFLAMAGVKVPDKFVLDGGNRSAEECAARCAGNCSCMAYAYAKLQSSSAKGDVRRCLLWAGDLVDTQMIGPLWASLADTLYLRVPLPPAGSAGTMASKNALKIALPVLAGVLLLACILFVWFCRFREKGRKTESQKKLVPGSANTSTEIGEGEHAEDLEFPSVRFVDIVAATGNFSKAFMIGRGGFGKVYKGTLESGREVAVKRLSKDSDQGTEEFKNEAILIAKLQHRNLVRLLGCCTEGAEKLLIYEYLPNKGLDAILFDSERKSVLDWPTRLEIIKGVARGLLYLHQDSRLTVIHRDLKASNVLLDAEMRPKIADFGMAKIFCDNQQNANTKRVVGTFGYIAPEYSTEGVFSVKSDVYSFGVLLLEIVSGVRISSPDDIMEFPSLIVYAWNLWREGKAGGLVDPSIAGSCSQEEALLCIHVGLLCVEGDPSRRPLMSAVVSILENGSGSSSSTLSLPKPNQPAYLALMEAKRDDLENTRNSITMTVLQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HUJ9</accession>
    <name>A0A0Q3HUJ9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_4g40865v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ91963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HUJ9"/>
    <dbReference type="STRING" id="15368.A0A0Q3HUJ9"/>
    <dbReference type="EnsemblPlants" id="KQJ91963">
        <property type="protein sequence ID" value="KQJ91963"/>
        <property type="gene ID" value="BRADI_4g40865v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ91963">
        <property type="protein sequence ID" value="KQJ91963"/>
        <property type="gene ID" value="BRADI_4g40865v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HUJ9"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18280">
        <property type="entry name" value="BTB_POZ_BPM_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF491">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
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        <location>
            <begin position="18"/>
            <end position="142"/>
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        <location>
            <begin position="179"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ91963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ91963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="40078" checksum="253113FF48E9A4EF" modified="2016-01-20" version="1">MAVDHRDLSSSIVAEAEKVSHVIKIDGYSITKGLRENGSCATSLPFKVAGHSWVVRYYPNGYRSEDAGYPPYASTTTKVILGFSLLDKDLTPVPSYSPKGPVPARTFSKQSPSWGHGRFIRKENLERSAHVRGDCFSIRCDVTVLKDIRVRENKVEFVVVPRSNLGRHLAGLLESMDGADVTFLVAGQRFSAHRCVLAARSSVFKAELFGAMREKEAGGVVEIGDMEADVFGSLLHFIYTDSLPEVTIGNEESAVDDVAMAQHLLVAADRYDVERLKLMCEDRLCRNIDSTVVATSLVLAEQHGCPGLKEACVEFLDSASNFEAMLTSDGFNHLKNSCPSLLSELIRRFMPAELNAANKDIVMAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IRN3</accession>
    <name>A0A0Q3IRN3_BRADI</name>
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        <recommendedName>
            <fullName evidence="2">DUF3615 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">BRADI_2g05107v3</name>
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    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK03028">
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        <property type="gene ID" value="BRADI_2g05107v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03028">
        <property type="protein sequence ID" value="KQK03028"/>
        <property type="gene ID" value="BRADI_2g05107v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="663959at2759"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK03028.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="261" mass="29044" checksum="838D61239063EDD0" modified="2018-03-28" version="2">MADKEETRPLVGVGPAPPGPLAKTAEISEIPPVRPMIEAGKEAEAEAEESCRLRGFGRKPRPLAGDGPAPPRTREARAQIEGVSHEIVLQCLEWYNSNHPGDEYEPAPGRVTRYTIINNLRWWTHGNFVARRKRSGSGCFSFLPAQRTLFFFELTAGLDCIDQVVTCAPLDEPVTEAYKFLGFRLGCGTRRDGGSDCVCKTCHRQFLLPDPFMTRTCACGGKVERLCHMCYPKCIVLHPLGGEFEFGHHKDNVWKAHLDHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JKI4</accession>
    <name>A0A0Q3JKI4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_1g05198v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JKI4"/>
    <dbReference type="EnsemblPlants" id="KQK12660">
        <property type="protein sequence ID" value="KQK12660"/>
        <property type="gene ID" value="BRADI_1g05198v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK12660">
        <property type="protein sequence ID" value="KQK12660"/>
        <property type="gene ID" value="BRADI_1g05198v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JKI4"/>
    <dbReference type="OrthoDB" id="662982at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="5">
        <location>
            <begin position="18"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK12660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK12660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="68" mass="8028" checksum="DE83AB0631A585CD" modified="2016-01-20" version="1">MATERLHLTFGPLPLINCTSCGFRRVKRYTSSTEENKDRDFVKCINHGPKFEGCDFWYWIDEYANFAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JU96</accession>
    <name>A0A0Q3JU96_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rx N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_1g58965v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JU96"/>
    <dbReference type="FunCoup" id="A0A0Q3JU96">
        <property type="interactions" value="492"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK21147">
        <property type="protein sequence ID" value="KQK21147"/>
        <property type="gene ID" value="BRADI_1g58965v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK21147">
        <property type="protein sequence ID" value="KQK21147"/>
        <property type="gene ID" value="BRADI_1g58965v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JU96"/>
    <dbReference type="OrthoDB" id="652192at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377">
        <property type="entry name" value="OS10G0134700 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33377:SF103">
        <property type="entry name" value="RX N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK21147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="55846" checksum="9CD5FB046597AE6C" modified="2016-01-20" version="1">MESQVSATLGEVLSRFISLLIDKYLAVPQLSKVETLQKLERLLLRILITVEEAEGRCITNQAMLRQLNILRGEMWRGYYMLDVFRYLAREEEKAKDHHASRSLAMSISNPGKRLPVSASTIKGKRELKQVLDSVGNLVTDMSEFVVFLKNYPPITRQPYSMHLFLGKCMFGRQVEMERTIAFLLQRDPPGELKMGVLPIVGQGKVGKSTLVEHVCGDARVQGELCNLKDGGTVKHQSDALNEGQILVVIELDGDIDEGAWRRLYSASRSFIPSGSKIIITSPSEDITRFGTTQAVRLNFLPREASWYFFKVLVFGSTDTEEQPKLASVAMEIFEEYFDLEVFNDFTGPFANTINIAGYLRANINIQHWRRILTRVRGTRLRNQCRLSTGPSDATRVENKHFYFYIPRAAKPTKDCVVDSHVRVELSHEDVTKVTMDEVVSGSAPPQERFDVVVWKSHLPPYYSYITSCEIRDSKCMLKWNNRCKKRKSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K068</accession>
    <name>A0A0Q3K068_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronate decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BRADI_2g11960v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the NAD-dependent decarboxylation of UDP-glucuronic acid to UDP-xylose. Necessary for the biosynthesis of the core tetrasaccharide in glycosaminoglycan biosynthesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-alpha-D-xylose biosynthesis; UDP-alpha-D-xylose from UDP-alpha-D-glucuronate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.35" evidence="5"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK04145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K068"/>
    <dbReference type="FunCoup" id="A0A0Q3K068">
        <property type="interactions" value="1736"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3K068"/>
    <dbReference type="EnsemblPlants" id="KQK04145">
        <property type="protein sequence ID" value="KQK04145"/>
        <property type="gene ID" value="BRADI_2g11960v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK04145">
        <property type="protein sequence ID" value="KQK04145"/>
        <property type="gene ID" value="BRADI_2g11960v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K068"/>
    <dbReference type="OrthoDB" id="331544at2759"/>
    <dbReference type="UniPathway" id="UPA00796">
        <property type="reaction ID" value="UER00771"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3K068">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033320">
        <property type="term" value="P:UDP-D-xylose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05230">
        <property type="entry name" value="UGD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000044">
        <property type="entry name" value="UDP-glucuronic acid decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000073">
        <property type="entry name" value="UDP-glucuronic acid decarboxylase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078:SF14">
        <property type="entry name" value="UDP-GLUCURONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="15">
        <location>
            <begin position="113"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK04145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK04145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="426" mass="46680" checksum="7822A1897424C1B4" modified="2016-01-20" version="1">MASELTYRGGAATGSASDGGEYSPKPSKPLSWLTRAARYATAEHRPLFALVGMLFAAALFTFSSSSSSAGYTSPSGPAAVSFKHLANIAHPSLHESVGGKMPLGLRRRGLRVLVTGGAGFVGSHLVDRLMERGDSVIVVDNFFTGRKGNVAHHLGNPRFEVIRHDVVEPILLEVDQIYHLACPASPVHYKRHKTNVVGTLNMLGLAKRIGARFLLTSTSEVYGDPLQHPQVETYWGNVNPIGVRSCYDEGKRTAETLTMDYHRGANLEVRIARIFNTYGPRMCIDDGRVVSNFVAQALRKEPLTVYGDGKQTRSFQYVSDLVEGLMKLMEGEHIGPFNLGNPGEFTMLELAKVVQDTIDSSASIEYRPNTADDPHKRKPDITRAKELLGWEPKVPLHEGLPLMVTDFRKRIFGDQEESTTTAGGMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3K0F4</accession>
    <name>A0A0Q3K0F4_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g35956v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3K0F4"/>
    <dbReference type="EnsemblPlants" id="KQK17668">
        <property type="protein sequence ID" value="KQK17668"/>
        <property type="gene ID" value="BRADI_1g35956v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK17668">
        <property type="protein sequence ID" value="KQK17668"/>
        <property type="gene ID" value="BRADI_1g35956v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3K0F4"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK17668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="192" mass="20755" checksum="93161FEEE1AC2C5A" modified="2016-01-20" version="1">MPIPLLPLTAAPDSPRAATPAFSPRRDTVSRGGCRVRVVVPLYSPNWLGSAPPRTRRRESAATRGGGERWPPGRPRSTRRLGVNVATEVFQDDLDPRAAWESTFSRVCEEEGDGDSRTRALLAVGALLLPAQLWIHDDKLLDSESMAVVGFRRRSGPIQGIGRPPQRVHGGATDLGRTEQYGGPDVGSMVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LD88</accession>
    <name>A0A0Q3LD88_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fucosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100846246</name>
        <name evidence="9" type="ORF">BRADI_4g32160v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in cell wall biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">Golgi stack membrane</location>
            <topology evidence="7">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glycosyltransferase 37 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ90517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578231.1">
        <property type="nucleotide sequence ID" value="XM_003578183.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LD88"/>
    <dbReference type="FunCoup" id="A0A0Q3LD88">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3LD88"/>
    <dbReference type="EnsemblPlants" id="KQJ90517">
        <property type="protein sequence ID" value="KQJ90517"/>
        <property type="gene ID" value="BRADI_4g32160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846246"/>
    <dbReference type="Gramene" id="KQJ90517">
        <property type="protein sequence ID" value="KQJ90517"/>
        <property type="gene ID" value="BRADI_4g32160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846246"/>
    <dbReference type="OrthoDB" id="428346at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LD88">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008107">
        <property type="term" value="F:galactoside 2-alpha-L-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009969">
        <property type="term" value="P:xyloglucan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11340:FF:000005">
        <property type="entry name" value="Galactoside 2-alpha-L-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004938">
        <property type="entry name" value="XG_FTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31889:SF89">
        <property type="entry name" value="FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31889">
        <property type="entry name" value="FUCOSYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03254">
        <property type="entry name" value="XG_FTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="61"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ90517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ90517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="553" mass="61229" checksum="8B0DA3B9E52D64C2" modified="2016-01-20" version="1">MDGKPAMRPPRRPPLPSSKRSAVWLVGLLVALCFFTLPLLLALSRGRPNLSDVSKMSISVTARHEDETSTQAEANSDPAAPGIREQRDRDRLLGGLLSPDISESTCHSRYRSSLHRKAPSPHSPSPYLVSRLRKYEALHRKCGPGTLFYKKSLMQLTSAHSMGLVDCNYLVWAPSSHLGDRVLSMASAFLYALLTRRVFLVHVTGDMAGLFCEPFPGASWELPSGFLVPNLTQLHRGSDSSYGNLLRANKVPAGVRGESLPSYAYVHLEHDHQQPDQLFFCDDDQTVLSKVNWLILRSNLYFTPGLFLVPQFEDELRWMFPARSTTFHHIGRYLFHPSNKVWDVITRYHASYMASYQEKIGLEITTFAWNPVPPDEYLKQITACTSQEKILPDVVDPASSVAHEEVSAASKAVLVSSAQPEYAEKLKSMYYEHGTVTGETVGVLQPGAGKLPKNEKALVEMFLQSFCDVSVVSGWSTVGYVGHGLAGIKPWLLLPPSNKTAVDPPCVQAASMDPCFHAPPSYDCRAKKNGDLGAVLRHVRHCEDVGSGLKLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3LNX6</accession>
    <name>A0A0Q3LNX6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Peroxidase</fullName>
            <ecNumber evidence="19">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BRADI_3g09087v3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in defense response to powdery meldew fungus.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16 19">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16 19">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="19"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3LNX6"/>
    <dbReference type="FunCoup" id="A0A0Q3LNX6">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3LNX6"/>
    <dbReference type="EnsemblPlants" id="KQJ94189">
        <property type="protein sequence ID" value="KQJ94189"/>
        <property type="gene ID" value="BRADI_3g09087v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ94189">
        <property type="protein sequence ID" value="KQJ94189"/>
        <property type="gene ID" value="BRADI_3g09087v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3LNX6"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3LNX6">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000009">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF220">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 19" id="KW-0349">Heme</keyword>
    <keyword evidence="12 19" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="8 16 19" id="KW-0408">Iron</keyword>
    <keyword evidence="5 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 19" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="70"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <location>
            <position position="292"/>
        </location>
        <ligand>
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            <end position="271"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="EnsemblPlants" id="KQJ94189"/>
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        <source>
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    <sequence length="365" mass="37382" checksum="596F14731A637B0F" modified="2016-01-20" version="1">MVLIVTIASYPVSGFLPPIEWLPSHLPLAHGRGPPPPPPVVMRPRPDLLLASAAVIAVALHALAGGSAAQLSAGFYSASCPTVHGVVRQVMSQAVMNDTRSGAAILRLFFHDCFVNGCDASLLLDDTATTPGEKGAGPNAGGSTFGFDVVDSIKTQVEAACPGVVSCADILAIAARDSVNLLGGPSWAVPLGRRDATAPNPSGAATDLPGPDSDLAALVAAFAAKGLTSRDLAALSGAHTVGMARCAHFRTHVYCDDNVSPAFASQQRQACPASGGDASLAPLDALSPNQFDNGYYRSLMSGAGLLRSDQELFNNGAVDSLVRLYGSNANAFSADFAASMITLGNISPLTGSTGEIRLDCRKVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
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    <name>A0A0Q3LWG6_BRADI</name>
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        </submittedName>
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        <name type="common">Purple false brome</name>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">Bd21</strain>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
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    </reference>
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        <citation type="submission" date="2018-08" db="UniProtKB">
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</entry>
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    <reference evidence="2 3" key="1">
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            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
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        <property type="gene ID" value="BRADI_1g24463v3"/>
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        <property type="gene ID" value="BRADI_1g24463v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3NEH8"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="113"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK15702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="217" mass="23124" checksum="9890A22D8915A2EF" modified="2016-01-20" version="1">MRENLDFLLTCLTPSLLPLPTSAPPPPSSAGAGLASARPRLPIAPPFLSSTGPASPSRPLLSPILDPIPEPPPVPSPLSSAAPAPVLLCLRAASPDPPPTTPASSDPPRAAASKIGEAGSAAPEPARRRICRRRPPLWSDPSPSCPSVAGSGLPYLKRLTQVLMQKESHLENFNHYNSTILEYLSFCLIMLVMTSQMHTCFDTGEIFYRGHQRCQVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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    <name>A0A0Q3RUA3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8039 domain-containing protein</fullName>
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    </protein>
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        <name evidence="3" type="ORF">BRADI_1g29363v3</name>
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        <lineage>
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            <taxon>Magnoliopsida</taxon>
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            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3RUA3"/>
    <dbReference type="FunCoup" id="A0A0Q3RUA3">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16578">
        <property type="protein sequence ID" value="KQK16578"/>
        <property type="gene ID" value="BRADI_1g29363v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16578">
        <property type="protein sequence ID" value="KQK16578"/>
        <property type="gene ID" value="BRADI_1g29363v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3RUA3"/>
    <dbReference type="OrthoDB" id="663173at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <source>
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        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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</entry>
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            <dbReference type="PubMed" id="20148030"/>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
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            <authorList>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <source>
            <strain evidence="2">Bd21</strain>
        </source>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
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        </source>
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            <begin position="10"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT60570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CGH7</accession>
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            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
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    <gene>
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    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
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        <lineage>
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            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
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    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT61129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CGH7"/>
    <dbReference type="FunCoup" id="A0A2K2CGH7">
        <property type="interactions" value="1456"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT61129">
        <property type="protein sequence ID" value="PNT61129"/>
        <property type="gene ID" value="BRADI_5g10630v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT61129">
        <property type="protein sequence ID" value="PNT61129"/>
        <property type="gene ID" value="BRADI_5g10630v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CGH7"/>
    <dbReference type="OrthoDB" id="731210at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CGH7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000950">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000158">
        <property type="entry name" value="pentatricopeptide repeat-containing protein At2g33680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF476">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="496"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="644"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT61129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT61129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="678" mass="74395" checksum="22CBB00FD4ED46BC" modified="2018-03-28" version="1">MAAAAASTSAATVSAISAHVAAGRLFAALDKLTPSWSSSPIPSCLYASLLRLATSHGSLSAARRIATHLAASSSASSTSRSSVPTFLFNRAIESLAACGSVADARELFDLMPLRDGGSWNAIITASSRAGHPSEALSLFSNMNSLGIRPKDATMASVLSCCAECLDLCGARQLHGHIAKRDFQSNVILGTALVDVYGNCFLLADARRAFDDILEPNAISWNVIVRRYHLAGMGDMAVDMFFRMLSAGVRPLGYTVSHAVLACRDNNALEEGRCIHAFVLRHGYEHHVHVRSSVVDMYAKCGAMDAAQSLFNLAPMKDMQMRQETREFDAITLGSVLSACTGILDIGKGEEVHAFAIKCGFFSSPILKNALVRMYSKCGCLRSAERLLLFEMGSERDSYSWNSLISGYERHSMSEAALYALTKMQSEVTPNQSTFSSALAACANIFLLKQGMQIHAYMIRKGYEIDDILRSVLIDMYCKCRQFDYSIRIFEARPSRDVILWNSMIFGCAYSGKGEYGLDLFDEMQKQGIKADSVTFLGALVSCISEGHVRLGRSYFTLMMDESIIPRIEHYECMIELLGKHGCMVELEDFVEHMPFEPTTAMWLRIFDCCREYGNRKLGERAAKCINDSNPLTPVQFVATVDYESNDGGREAESTSFSSEGEGCEELPFSLEGEARLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CJA2</accession>
    <name>A0A2K2CJA2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100841804</name>
        <name evidence="8" type="ORF">BRADI_5g25760v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT62114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CJA2"/>
    <dbReference type="FunCoup" id="A0A2K2CJA2">
        <property type="interactions" value="1856"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2CJA2"/>
    <dbReference type="EnsemblPlants" id="PNT62114">
        <property type="protein sequence ID" value="PNT62114"/>
        <property type="gene ID" value="BRADI_5g25760v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT62114">
        <property type="protein sequence ID" value="PNT62114"/>
        <property type="gene ID" value="BRADI_5g25760v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5395350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CJA2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725:SF14">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725">
        <property type="entry name" value="ZN-FINGER, C-X8-C-X5-C-X3-H TYPE-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="260"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="422"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="644"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="443"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="493"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="549"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="647"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="688"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="736"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="765"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="PNT62114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="PNT62114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="785" mass="87809" checksum="4B17B892EE630819" modified="2018-03-28" version="1">MRDLPAFYTSLFQGGITGVKKSVQGNKQLPGKDMCSRRTWELEEEGNCRSRKTELRAALAVLPGGARRIWNVTGSHADVAAGLRRREKARSAASSCWAEKGRRRRDPAARANLATGAVIMEEDGASAAEAAVPAATKPLTPEEEALRRNTDCVYFLASPLTCKKPIDGMFGAPSPGIPAVSSHYAAYNLGKQMVPCYYFQKGNCVKGDKCPFYHGPQSTGNNPPEQVAKVSSFPLEQPQTQKNDLLGIKESARTNNLIQRGGPIIDDRSKMAVDRPTANSAKTPADAIPAKLASSALKSLPKSEKLQSSMPAAKRSFRTSSGEDHPECYHNNLIESDPVQDWNEDYRPPADDDLPQNSREADELPGESSPGFDVLVDNDGDAAAYLHDEEDFGRDMYPVEDYEYAPADFEIPPHHERELFNRMGEQGPVGQIYDGYERKRRRTSSERNMDRPSHSERRSRHRDIGHVEIDGSDLRHRLRRRKINGSSGTSPERSGEHRRRDERYRERAYDGHHTHRDRHRSPRGSTLSSRLQGRIKLPGRSPDRVVTSSEKEQDRRPLRERLSPVKHMDVQGVRHREAGQHQERTLRRSSELPSSARNADGQHLRRNVTESLNFGRKANGRVESEASLDFEGPKPLSIILQKKRQATCGNGSSAHNGKQDKSAKVSHRQPESIVETEKEGYDNIVSSEEYKSRSGDEYKEESRIPAEGHGQSSSHGDKPETEDIIEVDPVVNQEADNYEQGEGESDYDAAEGQEYKSEDENAYQNDEDEFEDDDDFARKVGVVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CMB5</accession>
    <name>A0A2K2CMB5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC112272498</name>
        <name evidence="7" type="ORF">BRADI_4g12678v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT63177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024319281.1">
        <property type="nucleotide sequence ID" value="XM_024463513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CMB5"/>
    <dbReference type="EnsemblPlants" id="PNT63177">
        <property type="protein sequence ID" value="PNT63177"/>
        <property type="gene ID" value="BRADI_4g12678v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112272498"/>
    <dbReference type="Gramene" id="PNT63177">
        <property type="protein sequence ID" value="PNT63177"/>
        <property type="gene ID" value="BRADI_4g12678v3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155:SF1210">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23155">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="4">
        <location>
            <begin position="357"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="5">
        <location>
            <begin position="591"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="704"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="PNT63177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="PNT63177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1166" mass="128827" checksum="FC2E7B60B11D9294" modified="2018-03-28" version="1">MAITTVASLIPKLEDLLKEESKYNLSKGVIKHLTYVHALLERAQQAGDQAEEELERTQQAEEERELSEVMVDIVETFTSSLASATADPQGGRGLVPKVMRSKLHTAKAHREFASNVSDAVQLYHKARDRHARFKVHYSIPSRNNKQPPVDHKGGGISSREVSVSLPDQILTKVDEMCKIHGGLKDEEIDLLKKEMECIGPALRKVAQVPVEQLDEGTKAWIDDLTEACHDIINDFRAPVPVPPPPPLPVAAAAAGTGKKPIIGSSMKLNLAAAKARRRNVAQIRGFNKILLEVAERRQRYWCEIAATNTEGEDVDEGSNRNPPPEPLQDSECPFLGIDVHTDELVKLLTTSSSGLQQEPLKLVSVVGPAGVGKKTLAEAVYSRISTQFECTARVSLSNHQTNNKSRTTLLLDLLRQLRQTEVVSETEDETETGLIDKIRETLNNKRYLIVIEDIWSSVEWDSMRNLFENSHHGSAVLTTTCKVDIAEYVGGVYKLAPLTECEATRLFYRTLFVSEDKCAPGDLANICGKLIAECGSIPLAIIAKADLLATKPWTVMKSHKVHGSYDTERIFEDYYKKLPRRLKPCLLYFSMFQKGYEMSGENLLWMWLNEGFLEAQEGEMCLNELINKGLIEAVEVDAAGKALSCRLHDMLHDSIVSLSAEEKFATILDGKVGNLLETAVRLLSIQGNNKDAQAQQLLRQAPNVRSLVVSGDAITSLMHATPLEEFQGLRVLDLGRGSPDNSLKNDQLKGIGSLFFLRCLVLAGRRITEIPKETGNLKSLQTLDLRATIVKELPATVFQAKKLKRLYVNSRTKIPCGIGKMEDLEELGDMNVSKPDLLKDLCSLTKLILLGIAIWSWDESYGDAMVENLRSLLDKRIIQSLSIVTCCSLDFMNKLEAGNTKATAAGTATAAAGNNKATAAAATPAPSSLQKLEISQSALLGLPRWIISLQNLSSLSIEVHKLTEDMIVMLGKLQNLLALSLTAKHAPAHPHVWFDKSANGFRKLTGFHFSSNGMGKMFRSGAMPKLERLKLSFQASTTDHVVSEASVTQESDFGSTVASSAQSSSWAAFDFGLENLPASLEHVRVEINCFNANDRVVNQAEAAIRRAVSKSRMRPPNLVIKRVREEHMDEGITDKEVVKRKWSSWASKPNDATMHQEGKNQSRLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CR62</accession>
    <name>A0A2K2CR62_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100840043</name>
        <name evidence="12" type="ORF">BRADI_4g29622v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May function as histone H3 lysine demethylase and be involved in regulation of gene expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT64511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318842.1">
        <property type="nucleotide sequence ID" value="XM_024463074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CR62"/>
    <dbReference type="EnsemblPlants" id="PNT64511">
        <property type="protein sequence ID" value="PNT64511"/>
        <property type="gene ID" value="BRADI_4g29622v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840043"/>
    <dbReference type="Gramene" id="PNT64511">
        <property type="protein sequence ID" value="PNT64511"/>
        <property type="gene ID" value="BRADI_4g29622v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1667110at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CR62">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000026">
        <property type="entry name" value="Transcription factor jumonji domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF53">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjC" evidence="11">
        <location>
            <begin position="617"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="871"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="879"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT64511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="PNT64511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="927" mass="103783" checksum="CC927AA26A729272" modified="2018-03-28" version="1">MGAMDLHHRIGAGDWLYGFEDEDEEEDVPAVEESSDDEDWKLELEKKGRKRRRPRSRRPAPSKRRRVATPSPSPASSESASTSGASASRKPRPRGAGIKQGAARGDTPIDGPAVVVPEQDAAVEVDETAASTSGTSRRKGGRACHQCKRVKKKPGQMIECGRCDENIYCAPCIKKQYTEMSSTEVRAECPSCLGICNCKRCTAVVKQREPKSSGLRKCKSRISVTRSRRAGATAGNDHPFLNSNGISNTSVISDKVDTMDVRGDEVDAVTKIKYARYLLHYLLPCLRDLNKEQMVEIEKEAENQGLSVSQLSVEQADCRNDERVFCDNCRTSIFDLHRSCPNCSYELCIVCCKELRENELLGTCREELVSYPYRGIEYMHGGDPSPESKTFKETDISSNTMKWPAISDGIIHCPPTDHGGCGNHVLRLRQIFPKDWLNRLEMDAVQFSKKLETSDVSGYARECTCCTKNENARHASIRENSADNYLYCPTSDNGKTEDLTHFQSHWVKGEPVIVQGVLKKMPDLSWEPPHMWSEIHGANTSSDMENVKAIDCLSCCEVEIRTQDFFKGYSEGRMYENLWPEMLKLKDWPTSNHFEELLPSHGAKYIHSLPFQPYTNLKSGLLCLSALLPDDILKLDMGPKSYIAYGCAQELGRGDSVTKLHCDISDAVNVLMHTAKVTPSEEQEDAIKKLKGRHDAQNGKDCCGNVATDGNDTCHESYVDVDHISTRRCSEDDYGGALWDIFRREDITELKKYLIKHSKEFRHIYCSPVEKIFNPLHDETFYLTKEHKRKLKEEHGIEPWTFVQKLGEAVFIPAGCPHQVRNLKSCTKIALDFVSPENVNECLKLTQQFRMLPKNHRAKEDKLEVQLLIPDSATPGSVPSSPAASTTDDSSSGVASYSDAQLNTPMSSPSSLLVTHIPCRPNYNATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CTD0</accession>
    <name>A0A2K2CTD0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_4g39850v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CTD0"/>
    <dbReference type="STRING" id="15368.A0A2K2CTD0"/>
    <dbReference type="EnsemblPlants" id="PNT65288">
        <property type="protein sequence ID" value="PNT65288"/>
        <property type="gene ID" value="BRADI_4g39850v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65288">
        <property type="protein sequence ID" value="PNT65288"/>
        <property type="gene ID" value="BRADI_4g39850v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2CTD0"/>
    <dbReference type="OrthoDB" id="662514at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000030">
        <property type="entry name" value="Heat shock protein HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000541">
        <property type="entry name" value="Luminal-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT65288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="410" mass="44333" checksum="BEF03A8B265D1EA8" modified="2018-03-28" version="1">MDSPLPSRPFRLGSVIAVRLGNTRSCVSGYGNGHAGAPVFRFCIPSWVAFSDDGAALVGEAAKDHAAANPQAAVFGFKRLLGKTFGHEDVQRESFPYKIVYKETSSTGTGIQVAKKQLLHVEDVAAMVLAELKCRAEAFLGHKVHYAVLTVPSYFRDASRLAAFDAVTSAGLEPVRIIDEPTAAALAHGLHENEITKNVLVLHVGGGTAEATVLVHEDGVFEAIGTWHDAHLGGDDFDRRTANHFLQLIREKHSVDIGNDTAKLWKLRAECERAKKELSDRDVARVSVNSIVDGVDLFEPLTRAKFEELNHDLFGRIMSVVDKAMVGSELAEKKELVDEIVLVGGSSRMPRIQELISDYFGGRQVRIGTAAVEPDEAVVHGGALLSHPTAGGYECLGGERRQFGASTDLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CU83</accession>
    <name>A0A2K2CU83_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DCD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100834059</name>
        <name evidence="4" type="ORF">BRADI_4g44780v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2CU83"/>
    <dbReference type="EnsemblPlants" id="PNT65591">
        <property type="protein sequence ID" value="PNT65591"/>
        <property type="gene ID" value="BRADI_4g44780v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65591">
        <property type="protein sequence ID" value="PNT65591"/>
        <property type="gene ID" value="BRADI_4g44780v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2CU83">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013989">
        <property type="entry name" value="Dev_and_cell_death_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044832">
        <property type="entry name" value="NRP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46034">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46034:SF23">
        <property type="entry name" value="DCD (DEVELOPMENT AND CELL DEATH) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10539">
        <property type="entry name" value="Dev_Cell_Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00767">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51222">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCD" evidence="3">
        <location>
            <begin position="34"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="415"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="285"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT65591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="776" mass="85732" checksum="E676AEFEC94C391A" modified="2018-03-28" version="1">MSTLAMVKKKSSWSQVVTGRPTNLFASARNLHPQDLGAVIFGCTNSTIAECHSRQLFGLPRAHLSYVQNITEGLPLFLFNYDDRKLHGIYEAASNGKFCPESNAWTYNGSEKTSYPAQVAMRVRMWCIPLEESKFRNAIIGNYYQKMPAVPGQKPHFFRFELDHTQTRALMVMFTPSPSPIKFWTPPVAQLAQTGSEHLREPTPPPVWAQKTEGNNELKSEKVLVSYADMVKQNKLESEGVGMGDVDDQRNNDLKPKKVLLSYADMVKQRKFEVHGIGMGDVDDEHASSSKESSDGFDDLDCKETPPEREDHALSSEAVEVQQQSIQEGNGLSFTLVLQKLKALSVQQLSSDPYAYGAGTEHIDAYGCKDMQEIKDVFIEGHSSLPENLDTDIDQLSWGHSSSLLQGLDYESYSEAKLIDVVKELSERIEATEKKQACSNKEIKYLQGVNDRLLKRVADLKDTVKNLNSKIDPLSLDDSLNQFVEECLGSEDVIYLTGGFDGISFLSSLDSFSPSLDILTPLKPMTVGKSYASTVALDGKIFVLGGGDGACWFDTVDCYDRRHDDWTPCPALTHEKGSLAGVCLYGKIYAFGGGDGIGCFSDVEMFDPAQGKWIKCQPMLEKRFALAGAELNGAIYAVGGFNGIQYLSSGERLDPREPNWNMLPMMSTGRGCHTVAVLDEKIYSIGGYDANAGAMVATVEFFEPRMPSWVMVEPMNYTRGYHSSAVLGGSIFTFGGVKGEADTILDVVERYKEGCGWVNTGMKSIGRRCYCSAIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2CYL4</accession>
    <name>A0A2K2CYL4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_3g21206v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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        <scope>IDENTIFICATION</scope>
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                <person name="Harmon-Smith M."/>
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                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="8">Bd21</strain>
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            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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        <source>
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                <person name="Bruce D."/>
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                <person name="Wang M."/>
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                <person name="Vogel J.P."/>
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        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
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        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="85" mass="9548" checksum="E3C3210DC46CF1A2" modified="2018-03-28" version="1">MATGGAGDMSPAGDLRRRREMVERLGPQLDGWLEMGAGPYVSNGSCLWLVQLNPFKLCSRSICISDMVANTFYKKKKIYMFCHNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2023-09-13" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2D9U0</accession>
    <name>A0A2K2D9U0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_2g22221v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2D9U0"/>
    <dbReference type="EnsemblPlants" id="PNT71051">
        <property type="protein sequence ID" value="PNT71051"/>
        <property type="gene ID" value="BRADI_2g22221v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71051">
        <property type="protein sequence ID" value="PNT71051"/>
        <property type="gene ID" value="BRADI_2g22221v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2D9U0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="PNT71051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="10043" checksum="C4C5578AC144C400" modified="2018-03-28" version="1">MCPPPGRPSLQSIFRRRRLHLLEGFLQHCNLFSAATSVLLVPRCFSCQRLPFLGDCFDRDSLTGKMALPVILDRFNWSRGGRHSLGALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DAT8</accession>
    <name>A0A2K2DAT8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_2g26958v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT71396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DAT8"/>
    <dbReference type="EnsemblPlants" id="PNT71396">
        <property type="protein sequence ID" value="PNT71396"/>
        <property type="gene ID" value="BRADI_2g26958v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT71396">
        <property type="protein sequence ID" value="PNT71396"/>
        <property type="gene ID" value="BRADI_2g26958v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DAT8"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036043377" evidence="2">
        <location>
            <begin position="18"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="PNT71396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="PNT71396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="88" mass="9496" checksum="2AE07DE98FF1EB32" modified="2018-03-28" version="1">MFHVASMLLFFFRFVLPIDMQVPADIMNSNEDEAAGGAHEEDKSSDLIPQRSRRNQSAVQIPPAEIAVAAVPDADNVLQGASERLGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DDZ4</accession>
    <name>A0A2K2DDZ4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="9">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100837485</name>
        <name evidence="14" type="ORF">BRADI_2g45420v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9 11">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="9"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT72521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DDZ4"/>
    <dbReference type="FunCoup" id="A0A2K2DDZ4">
        <property type="interactions" value="2127"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DDZ4"/>
    <dbReference type="EnsemblPlants" id="PNT72521">
        <property type="protein sequence ID" value="PNT72521"/>
        <property type="gene ID" value="BRADI_2g45420v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72521">
        <property type="protein sequence ID" value="PNT72521"/>
        <property type="gene ID" value="BRADI_2g45420v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DDZ4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000007">
        <property type="entry name" value="Isocitrate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF26">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9 12" id="KW-0521">NADP</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="13">
        <location>
            <begin position="11"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="310"/>
            <end position="315"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="10">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="10">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="PNT72521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="PNT72521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="412" mass="45988" checksum="664CCCEA329912DB" modified="2018-03-28" version="1">MAFEKIKVANPIVEMDGDEMTRVFWQSIKDKLIFPFLDLDIKYYDLGVLHRDATDDKVTVEAAEATLKYNVAIKCATITPDEDRVKEFNLKQMWRSPNGTIRNIINGNLLLEPIICKNVPKLVPGWTKPICIGRHAFGDQYRATDAVLKGPGKLRLVFEGKEETVDLEVFSFTGAGGVALSMYNTDESIQGFAAASMATAYDKKWPLYLSTKNTILKKYDGRFKDIFQEVYEAEWKSKFEAAGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHFRVHQKGGETSTNSIASIFAWTRGLAHRAKLDDNARLLEFAQKLEDACVGTVESGKMTKDLALLVQGSSNVTRSHYLNTEEFIDAVAAELRTRLAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DG59</accession>
    <name>A0A2K2DG59_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-synthesizing GTPase</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100834891</name>
        <name evidence="11" type="ORF">BRADI_2g56414v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564734.3">
        <property type="nucleotide sequence ID" value="XM_003564686.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DG59"/>
    <dbReference type="STRING" id="15368.A0A2K2DG59"/>
    <dbReference type="EnsemblPlants" id="PNT73276">
        <property type="protein sequence ID" value="PNT73276"/>
        <property type="gene ID" value="BRADI_2g56414v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100834891"/>
    <dbReference type="Gramene" id="PNT73276">
        <property type="protein sequence ID" value="PNT73276"/>
        <property type="gene ID" value="BRADI_2g56414v3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="13"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Initiation factor eIF2 gamma C-terminal" evidence="10">
        <location>
            <begin position="339"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="PNT73276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="442" mass="47669" checksum="6026537E531ED485" modified="2018-03-28" version="1">MDIGTQTSPTPRICIAGLVGQSATGKSTLLETLLGNEPYGRMEPTLRLKCAYAKIYNCSSSHAECYRSCSRIVDIAEHQCDTPGHELNPVSVQKHVSFVDCPGNMLYVSTMLAGIMMTDGVLVLSKPSVDFYLAPTCAVLAAASIMGKSIVVLQNCTEEESANKTKIKESLRQIQQFTRLIVSIEVPIVRLSVRDKTSSKIAELCSRINNMSTPEQDVTSPALMIIANSTCDPEGSENSDETLLVTIHGRVMQGVISPNQETEVRPGIVKVGEDGKAVSCPIILKVVAIMEDAAVSGQVVALRAAANIRNDDQLKSVIKGGLGGHVLGQVGSLPAVYSALKVKFSLVRKMFNPYSEELEKVEAIEKDQDLLLTIGAMSTHGKVHSITEDMMVVSALTPPSCARKEERIFISRKQELAWNLIGHGEIIGGKSVAVVERSFPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DG73</accession>
    <name>A0A2K2DG73_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_2g56435v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DG73"/>
    <dbReference type="EnsemblPlants" id="PNT73281">
        <property type="protein sequence ID" value="PNT73281"/>
        <property type="gene ID" value="BRADI_2g56435v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73281">
        <property type="protein sequence ID" value="PNT73281"/>
        <property type="gene ID" value="BRADI_2g56435v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DG73"/>
    <dbReference type="OrthoDB" id="686981at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022059">
        <property type="entry name" value="DUF3615"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046527">
        <property type="entry name" value="PIR2-like_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33120">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33120:SF39">
        <property type="entry name" value="OS01G0314000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12274">
        <property type="entry name" value="DUF3615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20235">
        <property type="entry name" value="PIR2-like_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIR2-like helical" evidence="3">
        <location>
            <begin position="78"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3615" evidence="2">
        <location>
            <begin position="293"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="PNT73281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="464" mass="51777" checksum="A9E15FAE9E4DFEB0" modified="2018-03-28" version="1">MGGRHNRSRRRRAKAEAEAAILIASDSPEKAFPSSGASATFSERPVQRKIPFSKLTESSPMEEEEDPRRVLRRSLLGQIRGYYLDSISRLPTTDLTTTLARGLLIAGHCYGPLHPAHNILLNAVWHSPATGSMCPSSATRSLDGLVASLRYHCPALSHDDAIWHLNLSRADLRAAVASARGAAPSLFRPAELDVKAAFQVAAKTARHPKPAAFALFASSVIPSVERDAVSLLNNKRRLSSADILCLSTMLLPSPLPDELPHPPLQDRCRKAFEIITRKRKLFIIWYERWVKIADAALRKYAQQTGSHYQLHIIYGTGTLKDEFNLDRSFHINFMAWPKDDPSSAREAPAFFFAEAVRGPDPGFCEENVTSCCTVQPSPSEVDSCHSCLTKKFRIDHPDDYENFDGGLDCPITLEVDYRCFDPDRDIDLVEYLDQDFHRYVSSLPWLRGHKDDGDLSILGYCSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DGB4</accession>
    <name>A0A2K2DGB4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BRADI_2g56990v3</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="PNT73321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DGB4"/>
    <dbReference type="STRING" id="15368.A0A2K2DGB4"/>
    <dbReference type="EnsemblPlants" id="PNT73321">
        <property type="protein sequence ID" value="PNT73321"/>
        <property type="gene ID" value="BRADI_2g56990v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT73321">
        <property type="protein sequence ID" value="PNT73321"/>
        <property type="gene ID" value="BRADI_2g56990v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DGB4"/>
    <dbReference type="OrthoDB" id="122279at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A2K2DGB4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000287">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase RKF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000542">
        <property type="entry name" value="Receptor-like serine/threonine-protein kinase At4g25390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF63">
        <property type="entry name" value="OS01G0883000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="160"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="PNT73321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblPlants" id="PNT73321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="514" mass="55454" checksum="D490162EA83443AA" modified="2018-03-28" version="1">MPLCSACLDAGIRASARLTAKAGDRAKSLSCFYLTVLYAAGVASNAGPVSPATASCTLGLALSTPASPSSSSTATPSSSSSPISHTHIAVATTIPIASVLLVSLIALLLLWTKRRDDGIKRTSRNRLSEERSRSRASRERPNTGSVLFEFGELARATGGFAERNVIGRGGFGVVYRGVLPGDGSVVAVKKMLDPDVEGGDEEFTNEVEIISQFRHRNLVPLRGCCITTATNDADDDDDHQGDNKQMLLVYDYMPNGSLDRYIFAAQEGDGAAAALMPWAQRRSVILDVARGLEYMHHGVKPGIYHRDVKATNILLDADMRARVADFGLARRSRDGQSHLTTRVAGTHGYLAPEYALYGQLTEKSDVYSFGVLLLEAMSGRRALDLSDPSGMVLITDWAWARVRAGRAREVLAETLRREAGEEEECRPTSVQAAVAMVMERFVLVGILCAHVTVACRPTMPEALRMLEGDVDVPDLPDRPQPFGQRIAFDQGIEGSFSTSSVLSGPFVDFGDMLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DIK6</accession>
    <name>A0A2K2DIK6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g08113v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT74112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DIK6"/>
    <dbReference type="EnsemblPlants" id="PNT74112">
        <property type="protein sequence ID" value="PNT74112"/>
        <property type="gene ID" value="BRADI_1g08113v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT74112">
        <property type="protein sequence ID" value="PNT74112"/>
        <property type="gene ID" value="BRADI_1g08113v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DIK6"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT74112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT74112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="78" mass="8544" checksum="765FEE58CFCDBB12" modified="2018-03-28" version="1">MAGADPLEPAGRGNRELCFTSSVYPASVWSDESERRRGNILVINAASTVCQTLPLERSSSNRTSSVMSNEKRTGRRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DLK5</accession>
    <name>A0A2K2DLK5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g28245v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DLK5"/>
    <dbReference type="EnsemblPlants" id="PNT75155">
        <property type="protein sequence ID" value="PNT75155"/>
        <property type="gene ID" value="BRADI_1g28245v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT75156">
        <property type="protein sequence ID" value="PNT75156"/>
        <property type="gene ID" value="BRADI_1g28245v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75155">
        <property type="protein sequence ID" value="PNT75155"/>
        <property type="gene ID" value="BRADI_1g28245v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75156">
        <property type="protein sequence ID" value="PNT75156"/>
        <property type="gene ID" value="BRADI_1g28245v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DLK5"/>
    <dbReference type="OrthoDB" id="678303at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06222">
        <property type="entry name" value="RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044730">
        <property type="entry name" value="RNase_H-like_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052929">
        <property type="entry name" value="RNase_H-like_EbsB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074">
        <property type="entry name" value="BNAC02G40300D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47074:SF65">
        <property type="entry name" value="RNASE H TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="138"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT75156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="326" mass="36045" checksum="F8281E0686B4C2D0" modified="2018-03-28" version="1">MKISAQCPLCSTEAEDIHHMLFRCERAMEVWKALGCESFVQEARDSDRAGPAALDFLLGASFRSGSPPVGFKELATTACWYLWWERRKIVHEEPVQAPVRSALAIRALASNFVAAVPGNAKLKPQPWSRPLQDWVKLNVDASSDADGLCGTVGAVTRDHHANFVAAINWRLDFVADVEAAEAHTVRVGLEFALLAGCSRISISSDSSAVVDALSAGHQPYGAAAAIYEDCFSLLDDLLQFHIGHCLRDSNKVAHELARVAKYQLYNSWYDSPCLHCIFQPLLPINREIFCCQKKRSLPKGNPLSFDLLQTRATTGKPRAWAHGTWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DLU0</accession>
    <name>A0A2K2DLU0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100832820</name>
        <name evidence="9" type="ORF">BRADI_1g29097v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT75249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560273.1">
        <property type="nucleotide sequence ID" value="XM_003560225.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024317988.1">
        <property type="nucleotide sequence ID" value="XM_024462220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DLU0"/>
    <dbReference type="EnsemblPlants" id="PNT75248">
        <property type="protein sequence ID" value="PNT75248"/>
        <property type="gene ID" value="BRADI_1g29097v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT75249">
        <property type="protein sequence ID" value="PNT75249"/>
        <property type="gene ID" value="BRADI_1g29097v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832820"/>
    <dbReference type="Gramene" id="PNT75248">
        <property type="protein sequence ID" value="PNT75248"/>
        <property type="gene ID" value="BRADI_1g29097v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT75249">
        <property type="protein sequence ID" value="PNT75249"/>
        <property type="gene ID" value="BRADI_1g29097v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832820"/>
    <dbReference type="OrthoDB" id="670984at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="28"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="199"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="PNT75248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="PNT75248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="389" mass="42074" checksum="3F6D5C3A351E2E74" modified="2018-03-28" version="1">MSADAAVSERKTKPLLPEGLLLPASMVLVQVFTMGALLLSKLAFNVGMAPFVLLAYRNLIGGITVAPFAFYFERDMMKKVNLKVWGWISVNALFGIVLAMGLQYYGLRATNAAYSVNFLNVIPVVTFIIAIILRVEKLKIETCPGKMKVIGTVIVVGGTMVISLYRGKLLHLWPTHLLKPQLQAAGAASAIPNHHNMLIGTMFLSGSCLSYAFWFIIQARVSKDFPSTYFSTMLACASGTIQAVVIGIMIDRDPSSWALSWDLQLLTVVYSGVFNTGVTFCLISWAVARRGPTYPSMFNSLSLMITTVLDSVLLGTDVSVGSLLGGLLIILGLYAFLWGKGKEMQEQRKQISAAAANTERSKGSGAADGNGVDSVQVGKQEVRIRVEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2K2DVE6</accession>
    <name>A0A2K2DVE6_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Neprosin PEP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g76015v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2K2DVE6"/>
    <dbReference type="FunCoup" id="A0A2K2DVE6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A2K2DVE6"/>
    <dbReference type="EnsemblPlants" id="PNT78251">
        <property type="protein sequence ID" value="PNT78251"/>
        <property type="gene ID" value="BRADI_1g76015v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78251">
        <property type="protein sequence ID" value="PNT78251"/>
        <property type="gene ID" value="BRADI_1g76015v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A2K2DVE6"/>
    <dbReference type="OrthoDB" id="684544at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1320.10">
        <property type="entry name" value="Outer-capsid protein sigma 3, large lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053168">
        <property type="entry name" value="Glutamic_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004314">
        <property type="entry name" value="Neprosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589:SF190">
        <property type="entry name" value="NEPROSIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31589">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF239)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03080">
        <property type="entry name" value="Neprosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52045">
        <property type="entry name" value="NEPROSIN_PEP_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neprosin PEP catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="PNT78251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="PNT78251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="211" mass="23009" checksum="D1A3EC2C2F077B82" modified="2018-03-28" version="1" fragment="single">SIGGPNDNYYGLHVTMDVYGYELKPGQQTSTTIWVGHKGDGATASFNEIQVGWHIYPDHYGDSQPHFYTQWTRDGYEETGCFNMDCPGFVRANGAIVAPGDVIHPVSDVPSGRIQKITLRVLKDKTSGDWWVYYGFNKIPTGVGYFPSGISPNGGRGHAASLTDIQLIDEDGRSSPIMADLPKIVTNEKCHSITPIDHAKCLYGGPGGCVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3UJQ8</accession>
    <name>E3UJQ8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Probable metal-nicotianamine transporter YSL9-like</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="9">Yellow stripe-like 9 transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">YSL9</name>
        <name evidence="10" type="ORF">BRADI_5g17210v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2010-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yordem B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2011" name="Ann. Bot." volume="108" first="821" last="833">
            <title>Brachypodium distachyon as a new model system for understanding iron homeostasis in grasses: phylogenetic and expression analysis of Yellow Stripe-Like (YSL) transporters.</title>
            <authorList>
                <person name="Yordem B.K."/>
                <person name="Conte S.S."/>
                <person name="Ma J.F."/>
                <person name="Yokosho K."/>
                <person name="Vasques K.A."/>
                <person name="Gopalsamy S.N."/>
                <person name="Walker E.L."/>
            </authorList>
            <dbReference type="PubMed" id="21831857"/>
            <dbReference type="DOI" id="10.1093/aob/mcr200"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="5">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="HM443952">
        <property type="protein sequence ID" value="ADO64257.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001266881.1">
        <property type="nucleotide sequence ID" value="NM_001279952.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239579.1">
        <property type="nucleotide sequence ID" value="XM_010241277.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3UJQ8"/>
    <dbReference type="STRING" id="15368.E3UJQ8"/>
    <dbReference type="EnsemblPlants" id="KQJ83860">
        <property type="protein sequence ID" value="KQJ83860"/>
        <property type="gene ID" value="BRADI_5g17210v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83861">
        <property type="protein sequence ID" value="KQJ83861"/>
        <property type="gene ID" value="BRADI_5g17210v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826305"/>
    <dbReference type="Gramene" id="KQJ83860">
        <property type="protein sequence ID" value="KQJ83860"/>
        <property type="gene ID" value="BRADI_5g17210v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ83861">
        <property type="protein sequence ID" value="KQJ83861"/>
        <property type="gene ID" value="BRADI_5g17210v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826305"/>
    <dbReference type="eggNOG" id="ENOG502QQ2H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015477_2_0_1"/>
    <dbReference type="OMA" id="VILQSWT"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="E3UJQ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051980">
        <property type="term" value="F:iron-nicotianamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010039">
        <property type="term" value="P:response to iron ion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048316">
        <property type="term" value="P:seed development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF51">
        <property type="entry name" value="YELLOW STRIPE-LIKE 9 TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="494"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="539"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="589"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="625"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADO64257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ83860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="663" mass="72260" checksum="955E400EAE3865E7" modified="2011-01-11" version="1">MALHQREAGGGKEEKSAMYGDSSEPGPRHGSVPPWREQLTVRGLLVSIAVGTMYSVIVMKLNLTTGLNPTLNVSAALISFVMLRGWTQALARLGVAVRPLTRQENTVVQTCAVACYSIGAAGGFGSYLLGLNKKTYEMAGVDMEGNVGHKEPRIGWMIGFLLTVSFVGILALVPLRKVLVIDYKLTYPSGTATAVLINGFHAPQGDEVAKMQVSGFTKYFAISFFWSFFQWFYSGGDKCGFSQFPTFGLRAWKQTFFFDFNLTYVGAGMICPHLINLSLLLGSVLSWGLMWPLIGGLKGKWYPADLPESSMKSLQGYKAFICIALILGDGIYNFAKIIVSTTMNLLDKSKLKNTKKEEDILPLDELHRNEVFMRDGLPNWLACSGYLALSVVAIITIPLMFPELKWYYAVIAYLLAPALGFSNAYGAGLTDINMAYNYGKVALLILAATAGKESGVVAGLIGCGMVKNLTSISADLMQDFKTGHLTLSSPRSMLIAQIIGTAMGCIISPLTFFVFYNAFDIGNQDGPWKAPYALIYRNIAILGVEGFSALPMHCLQLCYGFFGFALVANVMRDFLPRKYGKWIPLPMAMGFPFLVGGSFAIDMCVGSLIVYIWRKIDRTKAGHMVPAVASGFICGDGLWIFPASLLALAKITPPMCMAFGSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1GM02</accession>
    <name>I1GM02_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100820885</name>
        <name evidence="11" type="ORF">BRADI_1g04930v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant LTP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK12617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003564265.1">
        <property type="nucleotide sequence ID" value="XM_003564217.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GM02"/>
    <dbReference type="STRING" id="15368.I1GM02"/>
    <dbReference type="EnsemblPlants" id="KQK12617">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093916" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="9">
        <location>
            <begin position="35"/>
            <end position="202"/>
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    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="122"/>
        </location>
    </feature>
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        <location>
            <begin position="145"/>
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            <begin position="149"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
            <dbReference type="EMBL" id="KQK12617.1"/>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK12617"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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    <sequence length="202" mass="20211" checksum="85C325F119C7522E" modified="2012-06-13" version="1">MAASASASGRSSAMLGLAMAAVLLAFGLVGPAGADFAADRAECADRLMGLATCLTFVEATATARAPTPDCCSGFKTVLGASKKCLCVLVKDRDEPALGLKINVTRAMNLPSACSIPATFSDCPKILNMSPDSKEAEIFKQYAKEHEGKNGTTTTTPAAAAATGTTGGKSANAAAAAGAGTQRRSTAFVGVVSALLASVFVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I1GP32</accession>
    <name>I1GP32_BRADI</name>
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        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100831083</name>
        <name evidence="9" type="ORF">BRADI_1g11020v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Bd21</strain>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK13565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KQK13568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003559511.1">
        <property type="nucleotide sequence ID" value="XM_003559463.4"/>
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    <dbReference type="AlphaFoldDB" id="I1GP32"/>
    <dbReference type="STRING" id="15368.I1GP32"/>
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        <property type="protein sequence ID" value="KQK13565"/>
        <property type="gene ID" value="BRADI_1g11020v3"/>
    </dbReference>
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        <property type="protein sequence ID" value="KQK13568"/>
        <property type="gene ID" value="BRADI_1g11020v3"/>
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    <dbReference type="OrthoDB" id="20524at2759"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="expression patterns" value="baseline"/>
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        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="term" value="P:meiotic spindle assembly checkpoint signaling"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Dual specificity protein kinase TTK"/>
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    </dbReference>
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        <property type="entry name" value="serine/threonine-protein kinase mph1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027084">
        <property type="entry name" value="Mps1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF21">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="374"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="85"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="315"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="321"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK13565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQK13568"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="721" mass="80114" checksum="BA81D30E8297CE86" modified="2012-06-13" version="1">MERRDNFLRPPAADNTPTAGKSITFPNAGDTATTNLTSSSGSSSSLTLSPPDFLRQVHAACKRQRPPSSQTMPPRAGRVLVARGEGPTKAGASPSVSQNPEGNAMQKQSGARGPSRLRNAVPDQKNVKAAVVLLASNRNESLTTPSVLSTITYTHIQNASQNAQQKGEIGLLVDRKKSSLDVSSSEMISQNALTAGDNFPMAEVAHDQGRKHQEPQIADAAVEMDVKYDAANLPQRGVEEACNQNHGEPMTRCSVIGSSVTAVSLHSGQNVRVLQSNQCPSPMQMPEYTVESSAVISGLAPPKQQVPNLANDVSNKAVCGNQDSLPSQGQALSANDQSTSARDVGTSQASRGQKERRKNNYDPEFFFKVNGKLYQKLGKIGSGGSSEVHKVISMERAIYALKKIKLKGRDYPTAYGFCQEIEYLNKLKGKSNIIQLIDYEVTDKNLLKEGSVSPRDGRIKDDRYIYMVLEYGEIDLAHMVAQKWKERNNSNMKIDENWLRFYWQQMLEAVNTIHEERIVHSDLKPANFMLVRGSLKLIDFGIAKAIMNDTTNIQRDSQVGTLNYMSPEAFMCNEQDSGGNIVKCGRPSDIWSLGCILYQMVYGKTPFADYKTFWAKFKAVTDRNHKINYEPVDNPWLIDLMQRCLAWDRDERWRIPQLLQHPFLVPLVAGELPRIDRDPCNMLMERVRVHWANPDVSRLCSELHDVIAKLEEDQCSPTTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I1GSL3</accession>
    <name>I1GSL3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ninja-family protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">ABI-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100841611</name>
        <name evidence="8" type="ORF">BRADI_1g22180v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a negative regulator of abscisic acid (ABA) response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Ninja family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003562743.1">
        <property type="nucleotide sequence ID" value="XM_003562695.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GSL3"/>
    <dbReference type="STRING" id="15368.I1GSL3"/>
    <dbReference type="EnsemblPlants" id="KQK15364">
        <property type="protein sequence ID" value="KQK15364"/>
        <property type="gene ID" value="BRADI_1g22180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100841611"/>
    <dbReference type="Gramene" id="KQK15364">
        <property type="protein sequence ID" value="KQK15364"/>
        <property type="gene ID" value="BRADI_1g22180v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100841611"/>
    <dbReference type="eggNOG" id="ENOG502QW6K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034695_0_0_1"/>
    <dbReference type="OMA" id="MDEMPCV"/>
    <dbReference type="OrthoDB" id="667358at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GSL3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031307">
        <property type="entry name" value="Ninja_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012463">
        <property type="entry name" value="Ninja_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032310">
        <property type="entry name" value="NLS_NINJA_AFP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032308">
        <property type="entry name" value="TDBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31413">
        <property type="entry name" value="AFP HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31413:SF31">
        <property type="entry name" value="NINJA-FAMILY PROTEIN AFP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07897">
        <property type="entry name" value="EAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16136">
        <property type="entry name" value="NLS_NINJA_AFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16135">
        <property type="entry name" value="TDBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ethylene-responsive binding factor-associated repression" evidence="6">
        <location>
            <begin position="29"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Tify" evidence="7">
        <location>
            <begin position="294"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="179"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK15364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="335" mass="34742" checksum="4D4AF636B5F3D06B" modified="2012-06-13" version="1">MASRDFLGRFGGEKGAASERAGGGGGGEAEEAVELSLGLSLGGCFGASSGRDAKKPRLQLARSSSIGSMCSLPAGGGGGAGSENDDLAAATPAPAPLMRTSSLPAETEEERWRRREMQSLKRLQAKRKRLERRNSMNSGKSGGGAGDAQEPLYPSAFQLRRSVVSQGSTSSGIPDQGGSAAAAAAAEARSTSSMETSSDNNNNGSNQSKSLPPPAPTPAGKPPAPPPNGTAKEQPPPPPPLRTLRSLTMRTTSTGDLRKSMMEDMPMVSSKVDGPNGKKIDGFLYKYRKGEEVRIVCVCHGNFLTPAEFVKHAGGGDVTNPLRHIVVNPSPSVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I1GTM2</accession>
    <name>I1GTM2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC100846157</name>
        <name evidence="23" type="ORF">BRADI_1g25187v3</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">Bd21</strain>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Bd21</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK15839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010237216.1">
        <property type="nucleotide sequence ID" value="XM_010238914.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GTM2"/>
    <dbReference type="EnsemblPlants" id="KQK15839">
        <property type="protein sequence ID" value="KQK15839"/>
        <property type="gene ID" value="BRADI_1g25187v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846157"/>
    <dbReference type="Gramene" id="KQK15839">
        <property type="protein sequence ID" value="KQK15839"/>
        <property type="gene ID" value="BRADI_1g25187v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846157"/>
    <dbReference type="eggNOG" id="ENOG502QSUU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_116_7_1"/>
    <dbReference type="OMA" id="KNSTYWR"/>
    <dbReference type="OrthoDB" id="1910371at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF181">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094078" description="Receptor-like serine/threonine-protein kinase" evidence="19">
        <location>
            <begin position="31"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="475"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="21">
        <location>
            <begin position="31"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="22">
        <location>
            <begin position="377"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="568"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="511"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="596"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KQK15839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="890" mass="96959" checksum="D9DC0A737975FF0C" modified="2012-06-13" version="1">MEHARLVTPTALAAVPFFFLFLLLCLHADAATTLLQGQSLGRNDKLVSPNGAFLLAFFVPRGGGDGSRAYLGVLYARAAEETVPWVANRDAPVSASSALYSATVTSSGQLQILEGDRVVWQTSNTPPSSSSGNNNNFTLTIQDTGNLVLGNGGQNTAPLWQSFDHPTDTFLPGMSITLDRRDGAVASNTLFTSWASPGDPAPGNFTLGQDPLGSAQLYIWRHTPGNTPNNSGIKYWRSGQWANTKFVGIPWRSLYVYGFRLAGDASRGSGTRGGVMSYTFSAYNESQFRFVLKPNGTETCYMLLESTGAWEVVWSQPTIPCHAYNTCGPNAGCAAADDHGRAAACKCLQGFEPRSEEEYYGRGNWTRGCVRSKPLTCSERNVEVSGGDAFAALPGVKLPDFAVWESTVGGADACKGWCLANCTCGAYSYSDGTGCLTWSGRDLVDVYKFPNGEGYDLHIKVPASLLETGAKRRRWTAVIVSVVTALAVVLAACGILLWKCRRRIGEKLGVGGREEKKPRPSMLHPRREAKNDFSGPKQQPDLEEAENGDSCELPLFPLETLAEATGGFSDSNKLGEGGFGHVYKGSLPGGEEVAVKRLSKSSGQGCEEFKNEVILISKLQHRNLVRILGCCIQGHEKMLVYEYMPNKSLDAFLFDPARRGLLDWKTRLSIIEGIARGLLYLHRDSRLRVVHRDLKASNILLDHDMNPKISDFGMARIFGGDQKQENTNRVVGTLGYMSPEYAMEGLFSVRSDVYSFGILVLEIITGQKNSSFHHMEGSLNIVGYAWQMWNADKGSELIDPSIRSSSASREALRCVHMALLCVQDHACDRPDIPYVVMALGSDSSVLPMPKPPTFTLQCTSSDREGFLGGNADYYESYSASDLTVTMLQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1GUQ1</accession>
    <name>I1GUQ1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100820854</name>
        <name evidence="13" type="ORF">BRADI_1g28520v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560246.1">
        <property type="nucleotide sequence ID" value="XM_003560198.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GUQ1"/>
    <dbReference type="FunCoup" id="I1GUQ1">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GUQ1"/>
    <dbReference type="EnsemblPlants" id="KQK16388">
        <property type="protein sequence ID" value="KQK16388"/>
        <property type="gene ID" value="BRADI_1g28520v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100820854"/>
    <dbReference type="Gramene" id="KQK16388">
        <property type="protein sequence ID" value="KQK16388"/>
        <property type="gene ID" value="BRADI_1g28520v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100820854"/>
    <dbReference type="eggNOG" id="KOG0663">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_11_1"/>
    <dbReference type="OMA" id="TCEDEEY"/>
    <dbReference type="OrthoDB" id="595812at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000559">
        <property type="entry name" value="Protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000508">
        <property type="entry name" value="Protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF432">
        <property type="entry name" value="OS10G0154500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="37"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK16388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="312" mass="34229" checksum="5577C9FD262351C4" modified="2012-06-13" version="1">MSAASRKRAAAAADQEPRVDGGNKKRPRYAFGSIYDYEKLEVLGEGAYGQVLMARDRRSGKKVAVKWIRGCGDGGHGPPDFRALIREAGLQAACRGHPSIVEILDVVGDAKTGDMFIVMELVAGGRSLREEIWRPLSEHVTRVMMRQLVDAAKKIHGAGIIHRDIKPENVLVGMFGGLKVCDFGAATRQKPAGVPYEDSRVGTMIYTSPEQLAGNRNYGTAVDMWALGCVMAELLGGETLFEAESEKDMLAEVSKLREQIESTGKLDREWFAELSEPGREVLMGMLAFCPEERLTAAQVLEHRWFSKSSVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1GV47</accession>
    <name>I1GV47_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">15-cis-phytoene synthase</fullName>
            <ecNumber evidence="4">2.5.1.32</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100844630</name>
        <name evidence="10" type="ORF">BRADI_1g29590v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34475"/>
            <dbReference type="ChEBI" id="CHEBI:27787"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.32"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Plastid</location>
            <location evidence="9">Chloroplast</location>
            <location evidence="9">Plastoglobule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phytoene/squalene synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.32" evidence="4"/>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239422.1">
        <property type="nucleotide sequence ID" value="XM_010241120.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239427.1">
        <property type="nucleotide sequence ID" value="XM_010241125.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GV47"/>
    <dbReference type="SMR" id="I1GV47"/>
    <dbReference type="FunCoup" id="I1GV47">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1GV47"/>
    <dbReference type="EnsemblPlants" id="KQK16627">
        <property type="protein sequence ID" value="KQK16627"/>
        <property type="gene ID" value="BRADI_1g29590v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16628">
        <property type="protein sequence ID" value="KQK16628"/>
        <property type="gene ID" value="BRADI_1g29590v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK16629">
        <property type="protein sequence ID" value="KQK16629"/>
        <property type="gene ID" value="BRADI_1g29590v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100844630"/>
    <dbReference type="Gramene" id="KQK16627">
        <property type="protein sequence ID" value="KQK16627"/>
        <property type="gene ID" value="BRADI_1g29590v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16628">
        <property type="protein sequence ID" value="KQK16628"/>
        <property type="gene ID" value="BRADI_1g29590v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16629">
        <property type="protein sequence ID" value="KQK16629"/>
        <property type="gene ID" value="BRADI_1g29590v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100844630"/>
    <dbReference type="eggNOG" id="KOG1459">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037269_2_0_1"/>
    <dbReference type="OMA" id="NEAYNRC"/>
    <dbReference type="OrthoDB" id="6600518at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GV47">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010287">
        <property type="term" value="C:plastoglobule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046905">
        <property type="term" value="F:15-cis-phytoene synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000004">
        <property type="entry name" value="Phytoene synthase chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044843">
        <property type="entry name" value="Trans_IPPS_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31480">
        <property type="entry name" value="BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01212">
        <property type="entry name" value="Phytoene_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK16627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="440" mass="49063" checksum="B35B8DF4A96FE43A" modified="2012-06-13" version="1">MAATITLIGAASPGLGDAGCRDTGGTAGGHHGSFHCSRLLLPNNKRRRPPVAPRRWVLCSLKYACLGSVGEPGEAAAGRTSMTSPVYSSLTVSPAGEAAVAVVSSEQKVYDVVLKQAALLKRQLRPATLAGVRAREPLEVPLNGLKEAYGRCGEICEEYAKTFYLGTLLMTEERRRAIWAIYVWCRRTDELVDGPNASHITPQALDRWEKRLEDLFAGRPYDMLDAALSDTITRFPIDIQPFKDMIDGMRTDLRKARYKNFDELYMYCYYVAGTVGLMSVPVMGIEPESKATAESVYGAALALGIANQLTNILRDVGEDARRGRIYLPQDELAEAGLSDEDIFSGVVTNKWRNFMKRQIKRARMFFEEAERGVTELRKESRWPVWASLLLYRQILDEIEANDYNNFTRRAYVGKGKKLLALPVAYGKSLLLPYSLRNNQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1GVT9</accession>
    <name>I1GVT9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cryptochrome DASH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100845878</name>
        <name evidence="10" type="ORF">BRADI_1g31670v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May have a photoreceptor function.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </cofactor>
        <text evidence="7">Binds 1 5,10-methenyltetrahydrofolate (MTHF) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5 7">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010240284.1">
        <property type="nucleotide sequence ID" value="XM_010241982.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GVT9"/>
    <dbReference type="STRING" id="15368.I1GVT9"/>
    <dbReference type="EnsemblPlants" id="KQK16964">
        <property type="protein sequence ID" value="KQK16964"/>
        <property type="gene ID" value="BRADI_1g31670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845878"/>
    <dbReference type="Gramene" id="KQK16964">
        <property type="protein sequence ID" value="KQK16964"/>
        <property type="gene ID" value="BRADI_1g31670v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0133">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010348_6_2_1"/>
    <dbReference type="OMA" id="KFWRCGP"/>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GVT9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000719">
        <property type="term" value="P:photoreactive repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014133">
        <property type="entry name" value="Cry_DASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02765">
        <property type="entry name" value="crypto_DASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF22">
        <property type="entry name" value="CRYPTOCHROME DASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="9">
        <location>
            <begin position="87"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="352"/>
            <end position="356"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="392"/>
            <end position="399"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="489"/>
            <end position="491"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="423"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="476"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK16964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="587" mass="65917" checksum="2A8143632042A4A8" modified="2012-06-13" version="1">MLHHLSSSSCFPSTAQSLLLLPRSTANLRVLLTMSHDASSSSSSSRPLHGAAAVAVPSLGADETAATADEAFRRHTSPGLRRGGGGGVAVVWFRNDLRVLDNEALVRAWAAAEAVLPVYCVDPRIFAGVTHRFGFPKTGALRAQFLIECLEDLKQNLQKRGLNLLVRHGKPEDILPAIAKAVRAHTVYAHQETCSEEILVERLVHRGLEQVVIPQGPGGASNQNKPLNPKLQLIWGTTMYHVDDLLFPVSNLPDVYTQFRKAVESKSSVQNCCKLPPLLGPAPSSGLDELGGWGSIPTLESLGLSVTKSEKGMHFLGGESAALGRVHEYFWKKDQLKVYKETRNGMLGPDYSTKFSPWLASGSLSPRYICEEVKRYEKQRVANDSTYWVLFELIWRDYFRFLSVKYGSSIFHLGGPRKVVSKWSQDQVLFESWRNGRTGYPLIDANMKELLATGFMSNRGRQIVCSFLVRDMAIDWRMGAEWFETCLLDYDPASNYGNWTYGAGVGNDPREDRYFSIPKQAKTYDPDGEYVAYWLPELQSLPKERRNFPGASYIKQIIPLKFDGGHQKKDQQFNRQRKPNNIYRRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I1GW10</accession>
    <name>I1GW10_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100843827</name>
        <name evidence="2" type="ORF">BRADI_1g32287v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560437.1">
        <property type="nucleotide sequence ID" value="XM_003560389.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GW10"/>
    <dbReference type="STRING" id="15368.I1GW10"/>
    <dbReference type="EnsemblPlants" id="KQK17064">
        <property type="protein sequence ID" value="KQK17064"/>
        <property type="gene ID" value="BRADI_1g32287v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843827"/>
    <dbReference type="Gramene" id="KQK17064">
        <property type="protein sequence ID" value="KQK17064"/>
        <property type="gene ID" value="BRADI_1g32287v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843827"/>
    <dbReference type="HOGENOM" id="CLU_114280_0_0_1"/>
    <dbReference type="OMA" id="QWMVEND"/>
    <dbReference type="OrthoDB" id="694856at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
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        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="1">
        <location>
            <begin position="111"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK17064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="198" mass="22748" checksum="81508C27D27D9C2A" modified="2012-06-13" version="1">MWPLGFFGSLVAGRLLQYSRRICGQAALLGSAKLLHQPRRNLGDRVSRFGFDDRLPSHGSAVAMGALSLSMGGLLYFNKENAEEPLARKLIHQEELLEEETIEEKAMKKRFEQWMVENDRTYKGKEKAMRYEIFKREAELVDEHNARPGQSTTWGTNDFSDRTDEFRSGCGCSCRVWDEEVDDTESLTITKRHLPDAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I1GWH9</accession>
    <name>I1GWH9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100843415</name>
        <name evidence="6" type="ORF">BRADI_1g33670v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK17318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003560500.1">
        <property type="nucleotide sequence ID" value="XM_003560452.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1GWH9"/>
    <dbReference type="STRING" id="15368.I1GWH9"/>
    <dbReference type="EnsemblPlants" id="KQK17318">
        <property type="protein sequence ID" value="KQK17318"/>
        <property type="gene ID" value="BRADI_1g33670v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843415"/>
    <dbReference type="Gramene" id="KQK17318">
        <property type="protein sequence ID" value="KQK17318"/>
        <property type="gene ID" value="BRADI_1g33670v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843415"/>
    <dbReference type="eggNOG" id="ENOG502QQUR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015101_2_1_1"/>
    <dbReference type="OMA" id="CMPTYLT"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1GWH9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF317">
        <property type="entry name" value="OS06G0694200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094143" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="30"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK17318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="41037" checksum="2E9FD693436789F6" modified="2012-06-13" version="1">MASSAASSSSSVFALLGAVFALHLCCCHGRQGVAAIYSLGDSITDTGNLVKEAPPGAFETIKHLPYGVTLGYPTGRCSDGLLMIDFLAQDMGLPFLNPYLGKNKSFDHGVNFAVAGATAMDPAGLFGPRSFSMPFTVSSLKLQLRWFKDFLKSSFATDEDIRKRLQSSIVLVGEIGGNDYNYAFFTNKNVSDVEKLIPAVVQTIIDAAKEVLDMGASRVIIPGNFPIGCIPGYLTTMGSSEPSDYDSTGCLREMNLFAAKHNSKLQQAIAGLRSSYPNASIAYADYYNSFFSILKSASSLGFDANSTRMACCGAGGKYNYDERKMCGMEGTTACAEPSAYLSWDGIHMTQAAYKAMSRLIYHGRYLQPQILNFPEKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1H3Q1</accession>
    <name>I1H3Q1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7912 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100842923</name>
        <name evidence="3" type="ORF">BRADI_1g57350v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK20893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561424.1">
        <property type="nucleotide sequence ID" value="XM_003561376.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H3Q1"/>
    <dbReference type="FunCoup" id="I1H3Q1">
        <property type="interactions" value="482"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H3Q1"/>
    <dbReference type="EnsemblPlants" id="KQK20893">
        <property type="protein sequence ID" value="KQK20893"/>
        <property type="gene ID" value="BRADI_1g57350v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100842923"/>
    <dbReference type="Gramene" id="KQK20893">
        <property type="protein sequence ID" value="KQK20893"/>
        <property type="gene ID" value="BRADI_1g57350v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100842923"/>
    <dbReference type="eggNOG" id="ENOG502QTRC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055531_1_1_1"/>
    <dbReference type="OMA" id="SNKYGCP"/>
    <dbReference type="OrthoDB" id="1100432at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057234">
        <property type="entry name" value="DUF7912"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34544:SF3">
        <property type="entry name" value="OS07G0155200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34544">
        <property type="entry name" value="OSJNBA0006B20.18 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25498">
        <property type="entry name" value="DUF7912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7912" evidence="2">
        <location>
            <begin position="201"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK20893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK20893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="290" mass="32005" checksum="1520CF59A623E29B" modified="2012-06-13" version="1">MARAQALRSLLARCSTKCPAAAAAASSSCLRRAVPSYSQRILPAFRAASVPAVLTRSLATTARGGAGPDEPGAEEDEEEAEAQEWEEDEDYGTEPEIGDGGNGGGVLLRDIKWGELALASAKEVLAEHFGDDVAMFAFKVSPKGYVYVRLDKLTNRYGCPGIEEIENFNRIYKQKLDEMIERGEIPLDLAVEISSPGAERLLKVPEDLDRFKDMAMKVQYFAEGDDLVSDQADGIFLLESVDIQAEHCVWKLANVTENRAGKGRPLSRKQRDWRLQTSFQSVRKVTLYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4C2</accession>
    <name>I1H4C2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100827736</name>
        <name evidence="8" type="ORF">BRADI_1g59200v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024313209.1">
        <property type="nucleotide sequence ID" value="XM_024457441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H4C2"/>
    <dbReference type="SMR" id="I1H4C2"/>
    <dbReference type="EnsemblPlants" id="KQK21181">
        <property type="protein sequence ID" value="KQK21181"/>
        <property type="gene ID" value="BRADI_1g59200v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100827736"/>
    <dbReference type="Gramene" id="KQK21181">
        <property type="protein sequence ID" value="KQK21181"/>
        <property type="gene ID" value="BRADI_1g59200v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_1_4_1"/>
    <dbReference type="OMA" id="MCENTEA"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK21181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="489" mass="52737" checksum="F20E455E5954499F" modified="2012-06-13" version="1">MGEQGAAAAPLLDGRSSNGGSGKEEEEQLIEGRRGWGEYEKEEAWGQVSFAAPMVATSMAFYAIPLVSVMYAGRIGDVELAGATLGNSWGTVTGIALMTGLSGALETLCGQGYGAKVYHMLGVYLQASIITSALFSVLVSILWLYTEPLLIFLHQDPEVSRMAAVFLRYTIPAQFAYGFIQCTLRFLQTQSVVMPLVAFSLLPLVFHVGITHASVHYLGLGFAGPAMSTSISLWLSFIMLATYVMLSERFKHTWGGFSTEAFQHVLPGLKLAVPSAVMVCFEYWAFEVLVLLAGLMPDSQMSTSIIAMCVNTEAISYMVTYGFAAAISTRVSNELGAGNIDKAKKALKVTLALSLLLGVTFLLLLGLGHNMWAGLFSHSDTVISAFASMTPFLIGSVVLDSTQGVLSGVSRGCGWQHLVAWTNLVAFYIIGLPLSLLLGFKLGFHTKGLWMGQICGLLCQNAVLLFITLRTKWERLELATSSKEGNFVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1H4H1</accession>
    <name>I1H4H1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100836921</name>
        <name evidence="19" type="ORF">BRADI_1g59600v3</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK21239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561526.1">
        <property type="nucleotide sequence ID" value="XM_003561478.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H4H1"/>
    <dbReference type="FunCoup" id="I1H4H1">
        <property type="interactions" value="598"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H4H1"/>
    <dbReference type="EnsemblPlants" id="KQK21239">
        <property type="protein sequence ID" value="KQK21239"/>
        <property type="gene ID" value="BRADI_1g59600v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836921"/>
    <dbReference type="Gramene" id="KQK21239">
        <property type="protein sequence ID" value="KQK21239"/>
        <property type="gene ID" value="BRADI_1g59600v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836921"/>
    <dbReference type="eggNOG" id="KOG1052">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007358_0_1_1"/>
    <dbReference type="OMA" id="WDFFQRS"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901701">
        <property type="term" value="P:cellular response to oxygen-containing compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000037">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000054">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000175">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000081">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094295" description="Glutamate receptor" evidence="17">
        <location>
            <begin position="24"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="648"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="831"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="18">
        <location>
            <begin position="466"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="865"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="914"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="882"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="914"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="930"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="756"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037090-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KQK21239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblPlants" id="KQK21239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="945" mass="101361" checksum="9BA22D47959033C6" modified="2012-06-13" version="1">MADLLLLLLRVLCLCSVVPLAVAAARPGNVTIGALFTFNSVIGRSAAAAIDLAVADVNRDAAVLNGTRLSVVAQDTKCSGFVGTIQALQLMEKKVVAVVGPQSSGIAHVVSHVVNELHVPLLTFAATDPALASSQYPYLLRAARGDDASQMAAVADIVAFYGWRQVAAVYADTDHGRGGVDALGDALEPHRARIALRAPFPPGAGSAAMADLLVQVSLAESRVIVVHVSPDSGLAVFSAARSLGMMAAGYVWIATDWLAAAIDSSSSSSHPANHPKSTMGLIQGVLTLRRYSPDSPAKRALAMASRSKHLNAYGLAAYDSVWTAARAIDAFLADGLDVTFSDDPRLQAEQSGSNSTLRLGALKVFDQGPRLLEKLLLSNSTGITGALRFGADRSLVDPVYEVLNVGGTGVRRVGFWSNRTRLSLAAPDTKKNGSSSQQGLYSVIWPGETTATPRGWVFPNNGRPLRIGVPWRTTYRQFVSKDGTSPDGASGYCVDVFKAAVSLLPYPVPFSFVLFGDGEKNPSYGDLVSKVANDEFDAAVGDVSIVTNRTRVVDFTQPYVESGLVIVSPVKEKNPNAWAFLKPFTGAMWAVTGAFFLFVGAVVWVLEHRFNPDFRGSPRKQLVTIFWFSFSTMFFAHRENTVSTLGRMVLIIWLFVVLIINSSYTASLTSILTVQQLSTGIQGLDGLIASAEPIGYQVGSFAKSYMMEELNVPESRLKELAIDDYASSLQLGPHNGGVAAIVDELPYVDLFLSTNCQFKTVGQEFTKSGWGFAFQRDSPLAVDLSTAILTLSENGDLQRIHDKWLNPGQCDGATQGADVTAAADRLNLGSFWGLFLISGVACFLALLVYFARILCQYGKYKLAGGEPPGEDDPSSSSSSVSPERKPPVRRPERLRSIRELMTFVDMKEAEVKRAIRRKSSDDGRRQPDRSIASSAGASSFSVSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1H6Z5</accession>
    <name>I1H6Z5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100831582</name>
        <name evidence="6" type="ORF">BRADI_1g66687v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558238.1">
        <property type="nucleotide sequence ID" value="XM_003558190.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H6Z5"/>
    <dbReference type="FunCoup" id="I1H6Z5">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1H6Z5"/>
    <dbReference type="EnsemblPlants" id="KQK22346">
        <property type="protein sequence ID" value="KQK22346"/>
        <property type="gene ID" value="BRADI_1g66687v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831582"/>
    <dbReference type="Gramene" id="KQK22346">
        <property type="protein sequence ID" value="KQK22346"/>
        <property type="gene ID" value="BRADI_1g66687v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100831582"/>
    <dbReference type="eggNOG" id="KOG1737">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007105_6_0_1"/>
    <dbReference type="OMA" id="MSEPLQY"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000009">
        <property type="entry name" value="oxysterol-binding protein-related protein 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000008">
        <property type="entry name" value="Oxysterol-binding protein-related protein 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF136">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="368"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK22346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="52274" checksum="CFAA17BDE4FAEB83" modified="2012-06-13" version="1">MGSKDQDLAASSGGGGGFFSSIAAGVRSWGTAVHKSVNGMVGYEGLEVINPDGGTEDAEAEAMRGRWKQEDRDSYWKMMSKYIGSDVTSLVTLPVIIFEPMTMLQKMAELMEYCELLDKADECEDPYMRLVYASIWAVSVYPAYQRMWKPFNPILGETYEMVNHQGITFIAEQVSHHPPMGVAHCENDHFTYDITSKLKTKFLGNSVEVYPVGRTRVTLKKSGVVLELIPPLTKVNNLIFGRTWVDSPGEMIMNNLTTGDKVVLYFQPCGWFGAGRYEVDGYVYSAAEEPKMMVTGKWNKFMSYQPCDQEGEPLPGTELKEIWRVAPTPPSDKYQYTYFAHKINSFDTAPKKLLPSDSRLRPDRYALEKGDMSKSGAHKSRLEEQQRAEKRTRDTKGEQFTPKWFNMTDEISPTPWGELEVYEYNGKYAKHRAAVDSSNVAEETDVSSIEFNPWQYSDSPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1H7R0</accession>
    <name>I1H7R0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100832213</name>
        <name evidence="2" type="ORF">BRADI_1g68957v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003561856.1">
        <property type="nucleotide sequence ID" value="XM_003561808.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H7R0"/>
    <dbReference type="SMR" id="I1H7R0"/>
    <dbReference type="STRING" id="15368.I1H7R0"/>
    <dbReference type="EnsemblPlants" id="KQK22714">
        <property type="protein sequence ID" value="KQK22714"/>
        <property type="gene ID" value="BRADI_1g68957v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832213"/>
    <dbReference type="Gramene" id="KQK22714">
        <property type="protein sequence ID" value="KQK22714"/>
        <property type="gene ID" value="BRADI_1g68957v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832213"/>
    <dbReference type="eggNOG" id="ENOG502QR92">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009751_0_0_1"/>
    <dbReference type="OMA" id="PDEHICT"/>
    <dbReference type="OrthoDB" id="567691at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16897:SF2">
        <property type="entry name" value="OS03G0226600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16897">
        <property type="entry name" value="OS10G0105400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="541"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="758"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="795"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="541"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="803"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="833"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="874"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="899"/>
            <end position="914"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK22714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1157" mass="130936" checksum="958CA0DD6085A65C" modified="2012-06-13" version="1">MAAEAEAETAVASASGSGIWSRRRDEITFDRLQKFWNDLPLQARQELLKLDKQTLIEQARKNFYCSRCNGLLLENFKSLQQEVSDIDCLSAVSDSRSRHREQDYSQDPSIHPWGGLATTKDGVLTLIGCFVKAKSLRVLQNVFDNARSREREREMLYPDACGGGGRGWISQGMASYSRGYGTRETCALHTAHLSCNTLVNFWSALCEQTRSSLLRMKEEDFIERLIYRFDSKRFCRDCRKNVIREFKELKELKRIRREPRCTSWFCIADTAFQCEVFEDAIIIDWHQSLSETNGSYHHFEWAIGTDEGQSDVFGFEDVGMKTQVHRNGIDLDQFEDYFITLRAWKLDGRYTELCVKAHALKGQSCVHHRLVVGDGFVTVTKGESIRSLFEHAEEAEEEDEDEAMDRDEHDLDGDGSHPQKHAKSPELAREFLLDAAAVIFKEQVEKAFREGAAQQNAQSVFVSLALKLLEERVHVACKEIITLEKQNKLLEEEEKEKREEQERRMRRRTKEREKKQRRKERLKEKERDKEIKLVEFKSSDNISSSTLSNSSTCTTDEFGNTFGSRDSASEEENNSMVVDMFTADTCTDQSSCREIIGENIVDCCSMVTKRPSVNSSESFTSEHSKSSRRNLRLRKEVPQDNSSCRYDDDRNEFGSIDNLQAQSTERMRNDARICNSVFSTNSRTRDRQDYNSCSCNHQEGYRTEDGCFSPTVTSGREMKIARKAGVDKLTVQYCRVDGTRERNAVQKLVWERTDTPKKTCLHGTNDTSGSVDKADSSKPVECDISGCQKLDTGCEPLSQASERPSDVHKSETNQSYEHSEKTQSACHDGTPMMNKQTCYSRDNGSSRHDEELTTNSASSDGSSSCMTEADRESSSSSMTSLSAHNPESSSSDSEESSERVNNITEAPSTRTASRSLLETCAGNGFREYHPKVRCPPRNDRFGHNVPPFQDQLLHHRNMHLPPYSLASVGPHSHSCAAPINGYFQYGQPPNFFSSPLGFRVPGNRSPDFPVQYNNVHRYPAPAFSCIQPEPIHKKSTSFRVMPPPPSYRHGTGQIGSHTHGDLNPDRHNSVLKPMGLKDAAQGDSKMQDRSASFSLFQFNLPIAPPAPPLSKDGKSGEIVARTPFAPVQAQPCSREQTDVKEYNLFSTDQTGYFHLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1H843</accession>
    <name>I1H843_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein RIK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Rough sheath 2-interacting KH domain protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100835047</name>
        <name evidence="6" type="ORF">BRADI_1g70000v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Bd21</strain>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK22905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010228976.1">
        <property type="nucleotide sequence ID" value="XM_010230674.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024316245.1">
        <property type="nucleotide sequence ID" value="XM_024460477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1H843"/>
    <dbReference type="STRING" id="15368.I1H843"/>
    <dbReference type="EnsemblPlants" id="KQK22905">
        <property type="protein sequence ID" value="KQK22905"/>
        <property type="gene ID" value="BRADI_1g70000v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100835047"/>
    <dbReference type="Gramene" id="KQK22905">
        <property type="protein sequence ID" value="KQK22905"/>
        <property type="gene ID" value="BRADI_1g70000v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100835047"/>
    <dbReference type="eggNOG" id="KOG1960">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028572_0_0_1"/>
    <dbReference type="OMA" id="ATPKPFW"/>
    <dbReference type="OrthoDB" id="397265at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1H843">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22471">
        <property type="entry name" value="KH-I_RIK_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000037">
        <property type="entry name" value="KH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056149">
        <property type="entry name" value="PRP5/DDX46/KHDC4_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031121">
        <property type="entry name" value="RIK/BLOM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15744">
        <property type="entry name" value="BLOM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15744:SF0">
        <property type="entry name" value="KH HOMOLOGY DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23469">
        <property type="entry name" value="KH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent RNA helicase PRP5/DDX46/KHDC4 KH" evidence="5">
        <location>
            <begin position="108"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="KHDC4/BBP-like KH-domain type I" evidence="4">
        <location>
            <begin position="228"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="485"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="493"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="546"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="587"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="626"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00070402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00081001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQK22905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK22905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="661" mass="70301" checksum="622DD628D7400FC6" modified="2012-06-13" version="1">MTEEGTPRASGEPTAATKQRKKRKWDQPAEDIVSAAAEAAAVAGLPVLNFGALSGVQFPGITTYPAAPLPNAIPAPYALPPQLTPSVLQSAAAAVQKLSQAKIPDELIAREIVINDADPSVRYKLTKRQTQDEIQRCTCTVIITRGKYHPPNGQPDGEKPLYLHISAGSQLKDTAERIKAVDRAASMIEEILKQGQMPETTSTHFPSIQSNKQAVHPFSASIFLGFDADPSLNVAARIRGPNDQYINHIMNETGVTVSLRGKGSGNMGGCHAEASQQPLHMYLSSVHLKSLEAAKVLAENLLDTIAAEFGASRISSSKVYGAVPPPQQLLTGVHTSVTTPDVHSTLGPYGSTGAAHSYAPTGVTSPMAVPSTTLQSGFPTYSGIPPPSNLVYPSQAANGGTLYNGYGGIYPQATPLQQVSLTLKHASSSTTQVVPVVSTSTSMATEVNSSAKLESDKRFQRRKFQELPIPDLQNSQQGSKFVKTGLDDLGKMSSPSATPKTKVQPGSNVMFPHDLQHMPSHLSVSTGMSPPPPPPKNMSPPSSKNMPPPPPKSMPPPPPKFPSNEVLRNESRHSAVKEPMAPPRSLDVSSVSPPKSWSAQLPSKDPREEKPSSASVSETLLKLMDYGDDDDDDDDDDIDETDSVPGGNTTPSPGQKPFWSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1HA09</accession>
    <name>I1HA09_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100845631</name>
        <name evidence="5" type="ORF">BRADI_1g76040v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK23775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="PNT78252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003558869.1">
        <property type="nucleotide sequence ID" value="XM_003558821.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HA09"/>
    <dbReference type="STRING" id="15368.I1HA09"/>
    <dbReference type="EnsemblPlants" id="KQK23775">
        <property type="protein sequence ID" value="KQK23775"/>
        <property type="gene ID" value="BRADI_1g76040v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT78252">
        <property type="protein sequence ID" value="PNT78252"/>
        <property type="gene ID" value="BRADI_1g76040v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100845631"/>
    <dbReference type="Gramene" id="KQK23775">
        <property type="protein sequence ID" value="KQK23775"/>
        <property type="gene ID" value="BRADI_1g76040v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT78252">
        <property type="protein sequence ID" value="PNT78252"/>
        <property type="gene ID" value="BRADI_1g76040v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100845631"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048352_0_0_1"/>
    <dbReference type="OMA" id="TASTHMI"/>
    <dbReference type="OrthoDB" id="1577640at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140693">
        <property type="term" value="F:molecular condensate scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000461">
        <property type="entry name" value="Ankyrin repeat domain-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24203">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24203:SF86">
        <property type="entry name" value="PROTEASOME 26S SUBUNIT, NON-ATPASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="300"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="366"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="399"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="81"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQK23775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQK23775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="468" mass="51964" checksum="18F0CD08F20C525D" modified="2012-06-13" version="1">MPASSLPLLLPFSSPIQTLNPLPASASCFHAPSKPLALSLCPRPLVSSSSSFAVAAVDDDDYDEDVALGDCLVFEDEAFEDPGLDLGRPSPSPPHRPTTSRRSRGKPISAAAGGGGGLVPERWKDAVEEINLTKKEKRRIAHGLRFGSRLDRRVPSAVAAPDEYRAYREGRLDAELGHVVRDYVEPLEDKSRAYREREARLGAEPGHVARDYVEPLEKSRAPEMIEAPPPPEPGTRAAPRNPRMGLDVGGLEDIAELFSSKEYAPSEMEDGKKRRPKLFTDEEKALLNKRIPDLESATSSKWLPLHTLAASGDFYLLDNLLKHKVDVNALDKDGLPAIHKAIISKKHAIINYLLRNSANPFIYDKDGATLMHYAVQTACHQTIKTLLLYNVDINRPDDYGWTPLHLAVQTQRTDIVRLLLIKGADRSSKTQDGLTPLELCLRLGHHVRTYELIKLLKSFRLQKQHDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I1HDT3</accession>
    <name>I1HDT3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC104582529</name>
        <name evidence="2" type="ORF">BRADI_2g08505v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010230686.1">
        <property type="nucleotide sequence ID" value="XM_010232384.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HDT3"/>
    <dbReference type="EnsemblPlants" id="KQK03548">
        <property type="protein sequence ID" value="KQK03548"/>
        <property type="gene ID" value="BRADI_2g08505v3"/>
    </dbReference>
    <dbReference type="GeneID" id="104582529"/>
    <dbReference type="Gramene" id="KQK03548">
        <property type="protein sequence ID" value="KQK03548"/>
        <property type="gene ID" value="BRADI_2g08505v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:104582529"/>
    <dbReference type="HOGENOM" id="CLU_2593047_0_0_1"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK03548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="80" mass="8732" checksum="8FC5B6FACBB46226" modified="2012-06-13" version="1">MSGDDGDKAKACGGGGEYETFRGPPSYPPPHPPVVGYPQPAPPPGLYGQREPYSRNRGGYQAGTGYPRYNCCDHWGYHWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I1HK36</accession>
    <name>I1HK36_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate dehydrogenase subunit 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100825203</name>
        <name evidence="1" type="ORF">BRADI_2g27360v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK06624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003568589.1">
        <property type="nucleotide sequence ID" value="XM_003568541.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HK36"/>
    <dbReference type="FunCoup" id="I1HK36">
        <property type="interactions" value="373"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HK36"/>
    <dbReference type="EnsemblPlants" id="KQK06624">
        <property type="protein sequence ID" value="KQK06624"/>
        <property type="gene ID" value="BRADI_2g27360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825203"/>
    <dbReference type="Gramene" id="KQK06624">
        <property type="protein sequence ID" value="KQK06624"/>
        <property type="gene ID" value="BRADI_2g27360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825203"/>
    <dbReference type="eggNOG" id="ENOG502S1RW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086191_0_0_1"/>
    <dbReference type="OMA" id="AQFLPRW"/>
    <dbReference type="OrthoDB" id="822750at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044963">
        <property type="entry name" value="SDH4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36358">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE SUBUNIT 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36358:SF1">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE SUBUNIT 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK06624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK06624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="24282" checksum="C8005A08F195B5A5" modified="2012-06-13" version="1">MASRFLARSKTLALALSRADAAPAPLSGSRALSSLPRYPVVSAPAPAVGSPTRVGKVLGYEPTSHLSGTQFSPRWFSTIVPSGSPMQKTQISETCKYVPGVEHSDALKATEGTSPKVVAFSPLEAAIAKPRSSPLTSESSKVSRSEIATQVTFYMIPALLLASKNSISTSLMVGAVYHQIYMFHKEIFLDYVHHDITRKWALIYFKLLLLVMAKDTIVYFDLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2022-05-25" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1HL09</accession>
    <name>C90D2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome P450 90D2</fullName>
            <shortName evidence="4">BdCYP90D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">3-dehydro-6-deoxoteasterone synthase</fullName>
            <shortName evidence="4">6-deoxotyphasterol synthase</shortName>
            <ecNumber evidence="3">1.14.14.147</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">6-deoxoteasterone synthase</fullName>
            <ecNumber evidence="3">1.14.14.-</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CYP90D2</name>
        <name evidence="6" type="ordered locus">BRADI_2g33050v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>cv. Bd21</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2020" name="J. Agric. Food Chem." volume="68" first="3912" last="3923">
            <title>Establishment of biosynthetic pathways to generate castasterone as the biologically active brassinosteroid in Brachypodium distachyon.</title>
            <authorList>
                <person name="Roh J."/>
                <person name="Moon J."/>
                <person name="Youn J.-H."/>
                <person name="Seo C."/>
                <person name="Park Y.J."/>
                <person name="Kim S.-K."/>
            </authorList>
            <dbReference type="PubMed" id="32146811"/>
            <dbReference type="DOI" id="10.1021/acs.jafc.9b07963"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in reduction steps of the biosynthesis of plant campesterol-derivative steroids, ending to castasterone (CS) but missing brassinolide (BL) (PubMed:32146811). Catalyzes the conversion of (22S,24R)-22-hydroxy-5alpha-ergostan-3-one (22-hydroxy-campesta-3-one, 22-OH-3-one) to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo-3-DHT), 3-epi-6-deoxocathasterone (3-epi-6-deoxoCT) to 6-deoxotyphasterol (6-deoxoTY) and of 6-deoxocathasterone (6-deoxoCT) to 6-deoxoteasterone (6-deoxoTE) (PubMed:32146811).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-epi-6-deoxocathasterone + reduced [NADPH--hemoprotein reductase] + O2 = 6-deoxotyphasterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:20717"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:59410"/>
            <dbReference type="EC" id="1.14.14.147"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:27322"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(22S,24R)-22-hydroxy-5alpha-ergostan-3-one + reduced [NADPH--hemoprotein reductase] + O2 = 3-dehydro-6-deoxoteasterone + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27325"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:20710"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:59411"/>
            <dbReference type="EC" id="1.14.14.147"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:27326"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>6-deoxycathasterone + reduced [NADPH--hemoprotein reductase] + O2 = 6-deoxoteasterone + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69811"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:20714"/>
            <dbReference type="ChEBI" id="CHEBI:20716"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:69812"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Plant hormone biosynthesis; brassinosteroid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.147" evidence="3"/>
    <dbReference type="EC" id="1.14.14.-" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HL09"/>
    <dbReference type="SMR" id="I1HL09"/>
    <dbReference type="STRING" id="15368.I1HL09"/>
    <dbReference type="EnsemblPlants" id="KQK07095">
        <property type="protein sequence ID" value="KQK07095"/>
        <property type="gene ID" value="BRADI_2g33050v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839691"/>
    <dbReference type="Gramene" id="KQK07095">
        <property type="protein sequence ID" value="KQK07095"/>
        <property type="gene ID" value="BRADI_2g33050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839691"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_15_5_1"/>
    <dbReference type="InParanoid" id="I1HL09"/>
    <dbReference type="OMA" id="KRCRRYG"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="UniPathway" id="UPA00381"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HL09">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102097">
        <property type="term" value="F:22alpha-hydroxysteroid 23-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016132">
        <property type="term" value="P:brassinosteroid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010268">
        <property type="term" value="P:brassinosteroid homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048366">
        <property type="term" value="P:leaf development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048441">
        <property type="term" value="P:petal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048443">
        <property type="term" value="P:stamen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000048">
        <property type="entry name" value="3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF226">
        <property type="entry name" value="CYTOCHROME P450 90D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0503">Monooxygenase</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000455310" description="Cytochrome P450 90D2">
        <location>
            <begin position="1"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P04798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="32146811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="32146811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="KQK07095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07095"/>
        </source>
    </evidence>
    <sequence length="510" mass="56818" checksum="3BA4FE5D70CA2CBF" modified="2012-06-13" version="1">MEALSMVGSGGVYSWPAALLVAAIVVSASVRWWGIKRQPTTTESKARLPRGSLGWPVVGETLAFISAAYSAQPESFVDKRRLLYGKVFKSHLWGSKAVVSSDAEVSRAVLQADASAFVPWYPSSLMQLMGESSILVLGGGLQRRVHGLAGAFFKSPQLKARLTVDMQRRVADAMDAWQCHGVVRVQDEAKSIVFEILVKALIGLEPGQEMHYLKQQFREFIAGLISLPIKLPGTQLYRSIQAKKRMAKLIQKIVQEKREKRMGGNNNATCKAPRDMIDVLMSNGSEELTDELISDNMIDFMIPAEDSVPVLITLAVKYLSECPLALEQLEEENMELKKRKSAVGGTLEWTDYMSLAFTQHVITETLRIGNIINGIMRKAVKDVEVKGQLLIPQGWCVFLYFRSVHLDGHIYDDPYAFNPWRWKERDMMAASSGFTPFGGGQRLCPGVDLARLEASIFLHHLVTTFRWEAEDDTVVTFPTVRLKRGMPIRVSQNIETSGTATFRSTESASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I1HLG9</accession>
    <name>I1HLG9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100838280</name>
        <name evidence="13" type="ORF">BRADI_2g34650v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May be a cell surface adhesion protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fasciclin-like AGP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566509.2">
        <property type="nucleotide sequence ID" value="XM_003566461.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HLG9"/>
    <dbReference type="STRING" id="15368.I1HLG9"/>
    <dbReference type="EnsemblPlants" id="KQK07334">
        <property type="protein sequence ID" value="KQK07334"/>
        <property type="gene ID" value="BRADI_2g34650v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100838280"/>
    <dbReference type="Gramene" id="KQK07334">
        <property type="protein sequence ID" value="KQK07334"/>
        <property type="gene ID" value="BRADI_2g34650v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100838280"/>
    <dbReference type="eggNOG" id="ENOG502QQ1S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067693_1_0_1"/>
    <dbReference type="OMA" id="IANSPWD"/>
    <dbReference type="OrthoDB" id="286301at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000006">
        <property type="entry name" value="Fasciclin-like arabinogalactan protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045003">
        <property type="entry name" value="FLA_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32077">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32077:SF84">
        <property type="entry name" value="OS05G0163300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014094678" description="FAS1 domain-containing protein" evidence="11">
        <location>
            <begin position="33"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="12">
        <location>
            <begin position="49"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK07334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="271" mass="27687" checksum="AE04DEFEF45DDB7B" modified="2012-06-13" version="1">MAMQTQTPSMSTPMILLLALLCLSAAARSAHAQTPAATAPAPATAAATPKTIKAVLTKAGQFTKFLQLLQSTQEDSQIDNQLKGKSSSGGLTVFAPPDNAFSALKSGTLNALSDAQKTSLVQFHVVSQLIPMAQFDTASNPLRTQAGETRPGKYPLNVTADGQQVNISTGVVNASVSGTVYTGDRLVVYQVDKVLLPWALYGPALPPAPAPSPVEKEKEKGKVGPKAAADAPAADAATASEAAVPARPGGVWGQGGVFGVAVAVAAAWFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I1HN52</accession>
    <name>I1HN52_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100832644</name>
        <name evidence="13" type="ORF">BRADI_2g39690v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein; which is present as a hexamer that forms the proton-conducting pore).</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Vacuole membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569121.1">
        <property type="nucleotide sequence ID" value="XM_003569073.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HN52"/>
    <dbReference type="FunCoup" id="I1HN52">
        <property type="interactions" value="1905"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HN52"/>
    <dbReference type="EnsemblPlants" id="KQK08111">
        <property type="protein sequence ID" value="KQK08111"/>
        <property type="gene ID" value="BRADI_2g39690v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832644"/>
    <dbReference type="Gramene" id="KQK08111">
        <property type="protein sequence ID" value="KQK08111"/>
        <property type="gene ID" value="BRADI_2g39690v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832644"/>
    <dbReference type="eggNOG" id="KOG0232">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085752_1_0_1"/>
    <dbReference type="OMA" id="MSVCPPY"/>
    <dbReference type="OrthoDB" id="2015590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000003">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="17"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="97"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQK08111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="165" mass="16605" checksum="CA1DD09947459825" modified="2012-06-13" version="1">MASVFSGDETAPFFGFLGAAAALVFSCMGAAYGTAKSGVGVASMGVMRPELVMKSIVPVVMAGVLGIYGLIIAVIISTGINPKAKPYFLFDGYAHLSSGLACGLAGLAAGMAIGIVGDAGVRANAQQPKLFVGMILILIFAEALALYGLIVGIILSSRAGQSRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HND3</accession>
    <name>I1HND3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100846102</name>
        <name evidence="16" type="ORF">BRADI_2g40500v3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003569165.1">
        <property type="nucleotide sequence ID" value="XM_003569117.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024314976.1">
        <property type="nucleotide sequence ID" value="XM_024459208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HND3"/>
    <dbReference type="FunCoup" id="I1HND3">
        <property type="interactions" value="1080"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HND3"/>
    <dbReference type="EnsemblPlants" id="KQK08213">
        <property type="protein sequence ID" value="KQK08213"/>
        <property type="gene ID" value="BRADI_2g40500v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK08214">
        <property type="protein sequence ID" value="KQK08214"/>
        <property type="gene ID" value="BRADI_2g40500v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846102"/>
    <dbReference type="Gramene" id="KQK08213">
        <property type="protein sequence ID" value="KQK08213"/>
        <property type="gene ID" value="BRADI_2g40500v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08214">
        <property type="protein sequence ID" value="KQK08214"/>
        <property type="gene ID" value="BRADI_2g40500v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100846102"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_59_0_1"/>
    <dbReference type="OMA" id="IWKPPTE"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000279">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000096">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000002">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF114">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="17"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="NAF" evidence="15">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KQK08213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="450" mass="50776" checksum="F62D66C5E693BFFC" modified="2012-06-13" version="1">MVGGGGGGGALRRVGKYEVGRTIGEGTFAKVKFAQNTETGESVAMKVLDRSSILRHKMVDQIKREISIMKLVRHPNVVRLHEVLASRKKIFIILEFITGGELFDKIIRHGRLAEADARKYFQQLIDGVDFCHSKGVYHRDLKPENLLLDSQGNLKISDFGLSAWPAEGASLLRTTCGTPNYVAPEVLSHKGYDGALADTWSCGVILYVLLAGYLPFDEVDLTTLYGKIESAEYSFPAWFPTGAKSLIRRILDPKPDSRIRIEEIRNDEWFKKNYEPVSEVENEEVNLDDVNAAFLDHEEDTEHTFDDEAGPLTLNAFDLIILSQGLNLASLFDRRQDHGKLQNRFLSRKPANVILSSMEVVAQSMGFKTHIRNYKMRVEGLNANKTSHLTILLQIFEVAPSIFMVELQRAAGDTSEYNKFLNNYCCKLDDIVWKFPVEKGKSRTSRLSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I1HNU8</accession>
    <name>I1HNU8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100837865</name>
        <name evidence="2" type="ORF">BRADI_2g41807v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003566803.1">
        <property type="nucleotide sequence ID" value="XM_003566755.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HNU8"/>
    <dbReference type="STRING" id="15368.I1HNU8"/>
    <dbReference type="MEROPS" id="S09.A31"/>
    <dbReference type="EnsemblPlants" id="KQK08428">
        <property type="protein sequence ID" value="KQK08428"/>
        <property type="gene ID" value="BRADI_2g41807v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837865"/>
    <dbReference type="Gramene" id="KQK08428">
        <property type="protein sequence ID" value="KQK08428"/>
        <property type="gene ID" value="BRADI_2g41807v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837865"/>
    <dbReference type="eggNOG" id="KOG4667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048353_0_0_1"/>
    <dbReference type="OMA" id="VIPNRRN"/>
    <dbReference type="OrthoDB" id="9988524at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1HNU8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF85">
        <property type="entry name" value="OS01G0579900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="40"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK08428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="280" mass="30870" checksum="290F1BBB72208544" modified="2012-06-13" version="1">MAQPLSPSSQDASNPQQRVVITNKHGEELVGLLHPVGSNNIVVLCHGFTASKNSSVIADLADALTKQGTSIFCFDFSGNGESGGEFQYGNYRKEADDLHSVVSYLHQKKYDVKAIVGHSKGGDVVVLYASMYHDVNMVVNLSGRFYLEKGIEERLGKGFIDRINKEGYIEVTKFLVKKKSGKILYRVTKESLMERLNTDMHATSLSISKECSIFTVHGSADEIVPVEDAYEFAKHIPTHKLCVIKGANHCYTAHRKELSNAVVDFIISNKAGDTSLPKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQ77</accession>
    <name>I1HQ77_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100826758</name>
        <name evidence="8" type="ORF">BRADI_2g46060v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010232115.1">
        <property type="nucleotide sequence ID" value="XM_010233813.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQ77"/>
    <dbReference type="STRING" id="15368.I1HQ77"/>
    <dbReference type="EnsemblPlants" id="KQK09101">
        <property type="protein sequence ID" value="KQK09101"/>
        <property type="gene ID" value="BRADI_2g46060v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826758"/>
    <dbReference type="Gramene" id="KQK09101">
        <property type="protein sequence ID" value="KQK09101"/>
        <property type="gene ID" value="BRADI_2g46060v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826758"/>
    <dbReference type="eggNOG" id="KOG0087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_23_0_1"/>
    <dbReference type="OMA" id="KACEIDH"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK09101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="219" mass="24049" checksum="E1FBBBF4DF0F92E7" modified="2012-06-13" version="1">MAVAYRAEEEYDYLFKVVLIGDSGVGKSNLLSRFARDEFSLDTRSTIGVEFATKTVQVDGKLVKAQIWDTAGQERYRAITSAYYRGAVGALVVYDVTRHITFENAERWLAELRDHTDANIVVMLVGNKADLRHLRAVPPEEARAFAERHRTFSMETSALESTNVEGAFTEVLGEIYRVVSKKALDIGDDPAAPPRGKTINVGAAKDEVSPVNTGGCCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1HQC8</accession>
    <name>I1HQC8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100836152</name>
        <name evidence="2" type="ORF">BRADI_2g46490v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK09176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014754470.1">
        <property type="nucleotide sequence ID" value="XM_014898984.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1HQC8"/>
    <dbReference type="FunCoup" id="I1HQC8">
        <property type="interactions" value="997"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1HQC8"/>
    <dbReference type="EnsemblPlants" id="KQK09176">
        <property type="protein sequence ID" value="KQK09176"/>
        <property type="gene ID" value="BRADI_2g46490v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836152"/>
    <dbReference type="Gramene" id="KQK09176">
        <property type="protein sequence ID" value="KQK09176"/>
        <property type="gene ID" value="BRADI_2g46490v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRHK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="NRHFQTN"/>
    <dbReference type="OrthoDB" id="2016402at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36329">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36329:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK09176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK09176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="38315" checksum="4CC048CFED1428BD" modified="2012-06-13" version="1">MQEASSSSLPALSTAYQPLPSLYLGFVAIWAASGLSWAFSSWRNRHFQANNLQWILALVPLIKALQMALSFLFWYSCVNLQTCSLWMSFGVYVTGILFQTASFVSFMLISHGYCIMYERLSIKERRTTAGLGCLLYLSLIGYKAAVPYFTVFLLINYFASFYIIFRRTSQCLLVLREQLNFVEEEDIHSLHGTLNTKYTMFKRFQGTMQVAAVAFIMVYMRADDTPDNYWFRVLVREWVQFCIFMYIGWNFRIPEASLNLPVMPLMKSNWEITMPPIYSVEMDAADFRGLVSDQWHVGVLATLQRSAHDDSCCSSQPLLVLVQNPSPKISGATTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1HZ43</accession>
    <name>I1HZ43_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aspartate aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100833284</name>
        <name evidence="10" type="ORF">BRADI_3g09050v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003571846.4"/>
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        <property type="protein sequence ID" value="KQJ94182"/>
        <property type="gene ID" value="BRADI_3g09050v3"/>
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    <dbReference type="EnsemblPlants" id="PNT66254">
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        <property type="gene ID" value="BRADI_3g09050v3"/>
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    <dbReference type="GeneID" id="100833284"/>
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        <property type="gene ID" value="BRADI_3g09050v3"/>
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        <property type="protein sequence ID" value="PNT66254"/>
        <property type="gene ID" value="BRADI_3g09050v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833284"/>
    <dbReference type="eggNOG" id="KOG1411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032440_1_2_1"/>
    <dbReference type="OMA" id="MQAVSVK"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0005739">
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
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        <property type="term" value="P:amino acid metabolic process"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Aspartate aminotransferase"/>
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        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
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        <property type="entry name" value="Aminotransferase_I/II_large"/>
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        <property type="entry name" value="Asp_trans"/>
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        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
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        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
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        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
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        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
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        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF45">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="58"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ94182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="430" mass="47736" checksum="0044F4C05E2D22C4" modified="2012-06-13" version="1">MAALSRAASAIRRRGALHLLPGGAPTRAMASLFGHVEPAPKDPILGVTEAFLADPSPDKVNVGVGAYRDDNGKPVVLDCVREAERRIAGNLNMEYLPMGGSIKMIEESLKLAYGEESEFIKDKRIAAVQALSGTGACRLFADFQKRFLPDSQIYIPTPTWSNHHNIWRDAQVPQRTFSYYHPESRGLDFAGLMDDIKNAPNGSFFLLHACAHNPTGVDPTEEQWREISYQFKLKNHFPFFDMAYQGFASGDPERDAKAIRIFLEDGHQIGCAQSYAKNMGLYGQRAGCLSILCEDEMQAVAVKSQLQQIARPMYSNPPVHGALVVSIILNDPDLKSLWLKEVKGMADRIIGMRKALRENLEKLGSPLSWEHVTNQIGMFCYSGMTPEQVDRLTNEYHIYMTRNGRISMAGVTTGNVAYLANAIHDVTKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1I087</accession>
    <name>I1I087_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100836465</name>
        <name evidence="15" type="ORF">BRADI_3g12957v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I087"/>
    <dbReference type="STRING" id="15368.I1I087"/>
    <dbReference type="EnsemblPlants" id="PNT66485">
        <property type="protein sequence ID" value="PNT66485"/>
        <property type="gene ID" value="BRADI_3g12957v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66486">
        <property type="protein sequence ID" value="PNT66486"/>
        <property type="gene ID" value="BRADI_3g12957v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66485">
        <property type="protein sequence ID" value="PNT66485"/>
        <property type="gene ID" value="BRADI_3g12957v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT66486">
        <property type="protein sequence ID" value="PNT66486"/>
        <property type="gene ID" value="BRADI_3g12957v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSE9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_15_0_1"/>
    <dbReference type="OMA" id="DCFTQFI"/>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I087">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000016">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000154">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000069">
        <property type="entry name" value="probable serine/threonine-protein kinase At5g41260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045845">
        <property type="entry name" value="BSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="75"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="PNT66485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="PNT66485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="507" mass="56898" checksum="993C43F0656B614E" modified="2012-06-13" version="1">MGARPWRLLCCCCCRGESDRNGVADDLRLKPDAAEEFVDGGEGETAAGDWCHDLPPFQEFSFQQLRLATAGFAAENIVSEHGDKAPNVVYKGKLDAQRKIAVKRFNRAAWPDPRQFLEEAKSVGQLRSKRFANLLGCCCEGDERLLVSEYMPNNTLAKHLFHWESKAMVGPMRLRVVLYLAEALDYCISKGRALYHDLNAYRVLFDDDCNPRLSCFGLMKNSRDGKSYSTNLAFTPPEYMRTGRITPESVIYSFGTLLSDVLSGKHIPPSHALDLIRDRNFSMLIDSCLAGQISNEEGTELLRLASRCLHYEPRERPNARSLVLALASLQKDVETPSYDLMDKPRGGAFTLQSIHLSPLAEACSRKDLTAIHEILEKTGYKDDEGTANELSFQMWTNQMQVTMDSKKKGDNAFRQKDFTVAIDCYSQFIDVGTMSSPTVYARRCLSHLMNDMPQQALNDAMQALVIFPTWPTAFYLQAAALFSLGKENEAREALKDGSAVETRSKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I1I0D2</accession>
    <name>I1I0D2_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">adenylate kinase</fullName>
            <ecNumber evidence="5">2.7.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100840739</name>
        <name evidence="13" type="ORF">BRADI_3g13360v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Bd21</strain>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="5"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571298.1">
        <property type="nucleotide sequence ID" value="XM_003571250.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I0D2"/>
    <dbReference type="STRING" id="15368.I1I0D2"/>
    <dbReference type="EnsemblPlants" id="KQJ94808">
        <property type="protein sequence ID" value="KQJ94808"/>
        <property type="gene ID" value="BRADI_3g13360v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100840739"/>
    <dbReference type="Gramene" id="KQJ94808">
        <property type="protein sequence ID" value="KQJ94808"/>
        <property type="gene ID" value="BRADI_3g13360v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100840739"/>
    <dbReference type="eggNOG" id="KOG3078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="YHEMLDG"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I0D2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097009">
        <property type="term" value="P:energy homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001694">
        <property type="entry name" value="Adenylate kinase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KQJ94808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ94808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="287" mass="31277" checksum="1105EEF809B06758" modified="2012-06-13" version="1">MASSTAAAAVSCLSTPPVTIERSIPRGRLLFRGSHAGGSRPLRLNSARRRGLSPAPRAAKAVEAAAKAGPLNVMIAGAPASGKGTQCELIKAKYGLVHISAGDLLRAEIAAGSQNGKQAKEFMEKGQLVPDEIVVNMVKERLLQSDAQEKGWLLDGYPRSNSQAMALETLGIRPDIFILLDVPDELLVERVVGRRLDPVTGKIYHLKYSPPENEEIASRLTQRFDDTEEKVKLRLQTHYQNIESLESIYEDVIVKVKGDTTVDDVFAEINKLLTSSFDKKTEMVAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1I3A0</accession>
    <name>I1I3A0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha</fullName>
            <shortName evidence="10">PFP</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">6-phosphofructokinase, pyrophosphate dependent</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">PPi-PFK</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Pyrophosphate-dependent 6-phosphofructose-1-kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100833293</name>
        <name evidence="10" type="synonym">PFP-ALPHA</name>
        <name evidence="12" type="ORF">BRADI_3g22340v3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Regulatory subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose 1,6-bisphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="2.7.1.90"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Allosterically activated by fructose 2,6-bisphosphate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Tetramer of two alpha (regulatory) and two beta (catalytic) chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade 'Long' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003573791.1">
        <property type="nucleotide sequence ID" value="XM_003573743.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I3A0"/>
    <dbReference type="FunCoup" id="I1I3A0">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I3A0"/>
    <dbReference type="EnsemblPlants" id="KQJ96294">
        <property type="protein sequence ID" value="KQJ96294"/>
        <property type="gene ID" value="BRADI_3g22340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100833293"/>
    <dbReference type="Gramene" id="KQJ96294">
        <property type="protein sequence ID" value="KQJ96294"/>
        <property type="gene ID" value="BRADI_3g22340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100833293"/>
    <dbReference type="eggNOG" id="KOG2440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022288_2_0_1"/>
    <dbReference type="OMA" id="AFPCTYG"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047334">
        <property type="term" value="F:diphosphate-fructose-6-phosphate 1-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009749">
        <property type="term" value="P:response to glucose"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.480:FF:000001">
        <property type="entry name" value="Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.480">
        <property type="entry name" value="Phosphofructokinase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01980">
        <property type="entry name" value="Phosphofructokinase_II_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011183">
        <property type="entry name" value="PfpB_PPi_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02477">
        <property type="entry name" value="PFKA_PPi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005482">
        <property type="entry name" value="PRK07085.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43650">
        <property type="entry name" value="PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43650:SF8">
        <property type="entry name" value="PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005677">
        <property type="entry name" value="PPi_PFK_PfpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="11">
        <location>
            <begin position="85"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048072"/>
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    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQJ96294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="617" mass="67152" checksum="C67471FAA17D4FAB" modified="2012-06-13" version="1">MNADYGASTELEGPLQQRRALYQPRLPPCLQGATVRVEYGDATTTIDPAGAPEVARAFPRTYGQPLVNFLAAAADKAAGERPPIRVGVVFSGRQSPGGHNLVWGLHDALKAHNPHCALYGFIGGTEGLFANKTLEITNDVLASYKNQGGFDLLGRSIDQIRTSKQLSAAMTTCTNLNLDGLVIVGGVTSNSDAAQLAETLVQNNCKTKVVGVPVSLNGDLKNQFVETTVGFDTVCKVNSQLISNVCLDAISAGKYYYFVRLMGRKASHVAFECALQSHPNMLIMGEEVALSKLTLMEIINKICDGVVARAELGKYHGVLLIPEGLIQSIPEMYALIQEISILHNNNVPVAEMSSQLSPWAAALFQFLPPFIRRELLLHQESDNSAQLSQIDTEQLLAHLVETEMTKRTKEGRYKGRKFSSVCHFFGYQARGSLPSNFDCDYAYALGHISLHIIAAGLTGYMATVANLKDSVDKWRCAAAPLTAMMSVKRHLRGPGAIPVGKPAIHPSPIDLKGKAYELLREKASSFLLDDFYRTPGGIQYEGPGSDAKPITLTIEDQDYIGDIEILKQYLDKVKVIVKPGCSRDILKAAISSMVSVTDVLTVMSHPLNCEMPLFHFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I1I3M6</accession>
    <name>I1I3M6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BRADI_3g23260v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ96472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I3M6"/>
    <dbReference type="STRING" id="15368.I1I3M6"/>
    <dbReference type="EnsemblPlants" id="KQJ96472">
        <property type="protein sequence ID" value="KQJ96472"/>
        <property type="gene ID" value="BRADI_3g23260v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ96472">
        <property type="protein sequence ID" value="KQJ96472"/>
        <property type="gene ID" value="BRADI_3g23260v3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1605014_0_0_1"/>
    <dbReference type="InParanoid" id="I1I3M6"/>
    <dbReference type="OrthoDB" id="1923497at2759"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="BT1"/>
    </dbReference>
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        <property type="entry name" value="FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FOLATE-BIOPTERIN TRANSPORTER 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03092">
        <property type="entry name" value="BT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KQJ96472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="166" mass="18694" checksum="6089A755D9935DEE" modified="2012-06-13" version="1">MVFANHLSTSFFLNLNSQMDVSHVPLPTLKSIRPWLWLTPCLVFPWLSVNFFLKDGIGASAASLQILQASPNLPMVAKLLLGLLSDVVPVRGYRRLPMVPSPTKIEGASSHLSCEPPLLQPLSVSIMYHVIFIFDPSCTMILEYNVTFYFGSIMYHDFVLLFWSTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5J3</accession>
    <name>I1I5J3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100846516</name>
        <name evidence="7" type="ORF">BRADI_3g31580v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Bd21</strain>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003571986.3">
        <property type="nucleotide sequence ID" value="XM_003571938.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5J3"/>
    <dbReference type="SMR" id="I1I5J3"/>
    <dbReference type="STRING" id="15368.I1I5J3"/>
    <dbReference type="EnsemblPlants" id="KQJ97526">
        <property type="protein sequence ID" value="KQJ97526"/>
        <property type="gene ID" value="BRADI_3g31580v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100846516"/>
    <dbReference type="Gramene" id="KQJ97526">
        <property type="protein sequence ID" value="KQJ97526"/>
        <property type="gene ID" value="BRADI_3g31580v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_1_0_1"/>
    <dbReference type="OMA" id="CMITMMF"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ97526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="562" mass="57793" checksum="E807C389FF2637C6" modified="2012-06-13" version="1">MCEGLIDRPLLSCRCNAKDGGGGVAVAPGNDVAKVVLSKPAAAAPVLADRPSSLTKATASKEAASILGLSLPMIMTGLILYVRPMISMLFLGRLGDLALAGGSLAMGFANITGYSVLSGLAAGMEPVCGQAVGAKNLPLVGATARRMVLLLLAASLPVGFLWAQMESLLLLCGQDASVSAMAQRYVLFSLPDLFFQCFLHPLRLYLRAQSINLPLTISATLAVAVHLPINYLFVTVLGLGVEGVALAAALTNLNLVLFLLAYVYVSGVHHATGGLFGFSSVSGFFEDVAGWARLARLAVESCASVCLEWWWYEIMVLLCGLLANPEATVASMGVLIQTTSLLYIFPSSLGYGVSTRVSNELGANRPRSARAAARAGLALAALQGVVSCMFAVAVRGVWARMFTSDAAILALTASVLPVLGMCELGNCPQTVGCGVLRGSARPSAGARINLGAFYGVGMPVALGLAFWAGMGFGGLWLGLLAAQAACVAVMLVVVGRTDWDRQAQLAQVLAGVAADDDVESGGGGGCVKGNVDGGKEVNVAAPHGDEDSSLLVTVVGLTGDMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1I5P8</accession>
    <name>I1I5P8_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BRADI_3g32070v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ97590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I5P8"/>
    <dbReference type="STRING" id="15368.I1I5P8"/>
    <dbReference type="EnsemblPlants" id="KQJ97590">
        <property type="protein sequence ID" value="KQJ97590"/>
        <property type="gene ID" value="BRADI_3g32070v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ97590">
        <property type="protein sequence ID" value="KQJ97590"/>
        <property type="gene ID" value="BRADI_3g32070v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822974"/>
    <dbReference type="eggNOG" id="ENOG502QR2X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027462_0_0_1"/>
    <dbReference type="OMA" id="MSMSHNW"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095044" description="Expansin" evidence="6">
        <location>
            <begin position="28"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="7">
        <location>
            <begin position="52"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="8">
        <location>
            <begin position="175"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KQJ97590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ97590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="265" mass="28132" checksum="3F1558C18060FAF0" modified="2012-06-13" version="1">MASAPSTATAILALLVSLAGVATTAHGRFTAMQWTPAHATFYGDETAAETMGGACGYGNLYATGYGTDTAALSTTLFLDGYGCGTCYQMRCVGAPACYQGSPVITVTATNLCPPNWEQDSNAGGWCNPPRTHFDLAKPAFMKMAAWRAGIVPVMYRRVPCVRKGGLRFAFQGNPHWLLVYVMNVAGAGDVRDMWVKGCDGGGHVQGWVRMSHNWGAAFQAFGQLGGHALSFKLTSYTTGQTIIAADAAPRTWSVGLTYQARANFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1I7U3</accession>
    <name>I1I7U3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABF2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100836269</name>
        <name evidence="11" type="ORF">BRADI_3g38200v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Bd21</strain>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2015" name="Plant Sci." volume="237" first="33" last="45">
            <title>Genome-wide analysis of SnRK gene family in Brachypodium distachyon and functional characterization of BdSnRK2.9.</title>
            <authorList>
                <person name="Wang L."/>
                <person name="Hu W."/>
                <person name="Sun J."/>
                <person name="Liang X."/>
                <person name="Yang X."/>
                <person name="Wei S."/>
                <person name="Wang X."/>
                <person name="Zhou Y."/>
                <person name="Xiao Q."/>
                <person name="Yang G."/>
                <person name="He G."/>
            </authorList>
            <dbReference type="PubMed" id="26089150"/>
            <dbReference type="DOI" id="10.1016/j.plantsci.2015.05.008"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="KJ850323">
        <property type="protein sequence ID" value="AJR27172.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001304807.1">
        <property type="nucleotide sequence ID" value="NM_001317878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I7U3"/>
    <dbReference type="STRING" id="15368.I1I7U3"/>
    <dbReference type="EnsemblPlants" id="KQJ98641">
        <property type="protein sequence ID" value="KQJ98641"/>
        <property type="gene ID" value="BRADI_3g38200v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836269"/>
    <dbReference type="Gramene" id="KQJ98641">
        <property type="protein sequence ID" value="KQJ98641"/>
        <property type="gene ID" value="BRADI_3g38200v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836269"/>
    <dbReference type="eggNOG" id="ENOG502QPP6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043238_1_0_1"/>
    <dbReference type="OMA" id="WNTEFSC"/>
    <dbReference type="OrthoDB" id="1927218at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I7U3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14707">
        <property type="entry name" value="bZIP_plant_BZIP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000048">
        <property type="entry name" value="ABSCISIC ACID-INSENSITIVE 5-like protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043452">
        <property type="entry name" value="BZIP46-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952:SF463">
        <property type="entry name" value="ABSCISIC ACID-INSENSITIVE 5-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952">
        <property type="entry name" value="CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="273"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="291"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AJR27172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ98641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="353" mass="37489" checksum="B43B1A5EF70DBE5D" modified="2012-06-13" version="1">MDLGDGGESERKGAAPPMPLARQGSVYSLTFDEFQSTLGGASGGGGLGKDFGSMNMDELLRSIWTAEESQAMASASAAPAGELQRQGSLTLPRTLSIKTVDEVWRDFVRDASPGAAGGGEPLPKRQPTLGEMTLEDFLVRAGVVRENPAAAAAVDAAVPPPLAARPIQVVNNGSMFFENFGGANGASGASAMGFAPVGIGDPSHPTMGNGMMPGVAGMGVGAVTVGPLDTSMGQLDSVGKVNGELSSPVEPVPYPFEGVIRGRRSGGHVEKVVERRQRRMIKNRESAARSRARKQAYTMELEAEVQKLKEQNEELQKKQEEMLEMQKNKALEVINNPYGHKKRCLRRTLTGPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1I8I1</accession>
    <name>I1I8I1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100839717</name>
        <name evidence="2" type="ORF">BRADI_3g40160v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ98949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003572376.1">
        <property type="nucleotide sequence ID" value="XM_003572328.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I8I1"/>
    <dbReference type="FunCoup" id="I1I8I1">
        <property type="interactions" value="356"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I8I1"/>
    <dbReference type="EnsemblPlants" id="KQJ98949">
        <property type="protein sequence ID" value="KQJ98949"/>
        <property type="gene ID" value="BRADI_3g40160v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839717"/>
    <dbReference type="Gramene" id="KQJ98949">
        <property type="protein sequence ID" value="KQJ98949"/>
        <property type="gene ID" value="BRADI_3g40160v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100839717"/>
    <dbReference type="eggNOG" id="KOG1603">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156987_0_0_1"/>
    <dbReference type="OMA" id="VEMVCIH"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47856">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 20-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47856:SF2">
        <property type="entry name" value="OS08G0512200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ98949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ98949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="89" mass="10160" checksum="D109A9C03AE3BA5C" modified="2012-06-13" version="1">MESTELKVEMVALHEKRVRKCLSKVKGVERVEVEGSIQKVVVTGYANRNKILKALRRVGLRAELWSPRNELLLTTYAAGSFAFNNYAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>I1I925</accession>
    <name>I1I925_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Retinoblastoma-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100826156</name>
        <name evidence="14" type="ORF">BRADI_3g41630v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Regulator of biological processes that recruits a histone deacetylase to control gene transcription. May play a role in the entry into mitosis, negatively regulating the cell proliferation. Formation of stable complexes with geminiviridae replication-associated proteins may create a cellular environment which favors viral DNA replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the retinoblastoma protein (RB) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010235349.2">
        <property type="nucleotide sequence ID" value="XM_010237047.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1I925"/>
    <dbReference type="FunCoup" id="I1I925">
        <property type="interactions" value="1694"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1I925"/>
    <dbReference type="EnsemblPlants" id="KQJ99188">
        <property type="protein sequence ID" value="KQJ99188"/>
        <property type="gene ID" value="BRADI_3g41630v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826156"/>
    <dbReference type="Gramene" id="KQJ99188">
        <property type="protein sequence ID" value="KQJ99188"/>
        <property type="gene ID" value="BRADI_3g41630v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826156"/>
    <dbReference type="eggNOG" id="KOG1010">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="VYCQSTQ"/>
    <dbReference type="OrthoDB" id="844594at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1I925">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
        <property type="term" value="P:regulation of DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000030">
        <property type="entry name" value="Retinoblastoma-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000067">
        <property type="entry name" value="Retinoblastoma-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.140:FF:000003">
        <property type="entry name" value="Retinoblastoma-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015030">
        <property type="entry name" value="RB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024599">
        <property type="entry name" value="RB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF17">
        <property type="entry name" value="RE32990P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11934">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01367">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01369">
        <property type="entry name" value="Rb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein N-terminal" evidence="11">
        <location>
            <begin position="100"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="12">
        <location>
            <begin position="427"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein C-terminal" evidence="13">
        <location>
            <begin position="886"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="879"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="672"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ99188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1036" mass="114411" checksum="E2DC220B82AC7C05" modified="2012-06-13" version="1">MEGADAPPPGTPGAGDDGSRSTAVSRTDDAATAAAMEQRFADLCKSKLALNESMMRQAMQLFKETKKILLSSMSSLGSGSPEEIQRFWSAFVLFCVSRLGKAGKAKEDGGITLHQILRAFEIKLVDFFKEMPQFCIKVGCVLTGLYGSDWEKKLELQELQATVVHLCSLGRHYKRAYQELFLSNDGKPADNPSEPNAQLASEYYRFGWLLFVLLRIQTNSRFKDLLTSITELVSVLAVLIVHIPVRLRKFSIEDSSFFAKKSDKGVNLIASLCEKHLTSADELRKSLEKTNTLIMDILEKKPCMDASECQQNNLSFIDPEGLTFFKNFMEEDSLKSSLLILDKEYENAINTKGELDERMFANDDDSFLGSGSLSGGAIKLPGTKRKYDVLASPSKSAISPSPMSPPRFCASPNGNSFGNSKMAPPLTPVSTAMTTAKWLRSTISPLPSRPSGELLRFFSACDKDVTDDITRRAAIILGAIFTGNSFGERMCTTVRSTNGFDAIWTEQRKMEALKLYYRVLESMCRAESHVLSGSNLTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHEDTLPRELKRHLNSLEERLLESLAWEKGSSMYNSLIVARPTLSVEINRLGLLAEPMPSLDAIVAHHDISLGGLPPLPFQKQERSPDKDEVRSPKRACTERRNVLVDSNSFRSPVKDTIKLKILPPLQSAFASPTRPNPAAGGETCAETGIGVFLSKIAKLAAIRIKGLCERLQLPQQILERVYSLVQQIISQQTALFFNRHIDQIILCSIYGVAKISQLSLTFKEIIFSYRKQPQCKLQVFRSVFVHRPSRSRTGKTGEDHVDIITFYNEVFIPTVKPLLVDLGSGASPNKNNGEKNATDAVPFPESPRLTRFPTLPDMSPKKVSATHNVYVSPLRSSKMDTLLSPSSKSYYACVGESTHAFQSPSKDLKAINTRLNSGKKVSGRLNFDVVSDLDVARSLSDQNGASTSMVVLGARSLGDQNGGYTPMKVLAAKPPVKCEQTDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I1IAB1</accession>
    <name>I1IAB1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Digalactosyldiacylglycerol synthase 1, chloroplastic</fullName>
            <ecNumber evidence="10">2.4.1.241</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100824823</name>
        <name evidence="14" type="ORF">BRADI_3g45180v3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + UDP-alpha-D-galactose = a 1,2-diacyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10520"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17615"/>
            <dbReference type="ChEBI" id="CHEBI:28396"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.4.1.241"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Plastid</location>
            <location evidence="9">Chloroplast outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.241" evidence="10"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ99777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IAB1"/>
    <dbReference type="FunCoup" id="I1IAB1">
        <property type="interactions" value="319"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IAB1"/>
    <dbReference type="EnsemblPlants" id="KQJ99777">
        <property type="protein sequence ID" value="KQJ99777"/>
        <property type="gene ID" value="BRADI_3g45180v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ99777">
        <property type="protein sequence ID" value="KQJ99777"/>
        <property type="gene ID" value="BRADI_3g45180v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ73">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011647_0_1_1"/>
    <dbReference type="OMA" id="IYRGWRE"/>
    <dbReference type="OrthoDB" id="44480at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IAB1">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046481">
        <property type="term" value="F:digalactosyldiacylglycerol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035250">
        <property type="term" value="F:UDP-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019375">
        <property type="term" value="P:galactolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01635">
        <property type="entry name" value="Glycosyltransferase_GTB-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000325">
        <property type="entry name" value="Digalactosyldiacylglycerol synthase 1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000218">
        <property type="entry name" value="Digalactosyldiacylglycerol synthase 1, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044525">
        <property type="entry name" value="DGDG1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46132:SF5">
        <property type="entry name" value="DIGALACTOSYLDIACYLGLYCEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46132">
        <property type="entry name" value="DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="6" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="13">
        <location>
            <begin position="541"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KQJ99777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ99777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="782" mass="88180" checksum="16FBB704F2BD17A5" modified="2012-06-13" version="1">MEAWVSPIAGAGGRGRGGDDSAFSFLSKGWREVRDSASADLRLMRARADSLRTLADRELEHLLASASTPLAAPPPPLAVGAPIAELEFVRKQIQPKISELRRQYASRDRELGRRVLERWVPPRGATSARVDLSGITAIRNAIVSEARDAEKWRRTAWNGEADTEEKEWEVVRMIRVGLKELERRSQSSELLGGFRGTGELVEKFKSSLKSFNMESEGYKEVPPLDITETLANLIRQSGPFLDQFGLRRDLCDKLVETLYSKQNHSLSAEATLVGNDSLADDLDLRIASVLQSTGYHTDDGFWNEPTKYEVSDTKRHIAVVTTASLPWMTGTAVNPLFRAAYLAKSEKQDVTLVVPWLCRSDQELVYPYSVTFNSPEEQETYIRNWVDERLGFASNFKISFYPGKFSKERRSILPAGDTSQFISSREADIAILEEPEHLNWYHHGKRWTEKFNHVVGIVHTNYLEYIKREKNGALQAFLVKHINNWVTRAYCDKVLRLSAATQDLPKSIVCNVHGVNPKFLKIGDKLTADREGGQQSFSKGAYFLGKMVWAKGYRELVDLLAKHKGDLEGFKLDVYGSGEDSQEVHSTARKLDLNLKFFKGMDHADDSLHRYKVFINPSISDVLCTATAEALAMGKFVVCAEHPSNEFFMSFPNCLTYKTSDEFVARVKEAMAREPQPLTPEERYNLSWEAATERFMEYSDLDKVLNDNSSPRLRETRSRRTSQPSFSNAVDGGLAFAHRCLTGSEVLRLATGAIPGTRDYDEQHCADMGLLPPQVQHPLYGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I1ICQ0</accession>
    <name>I1ICQ0_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC100825822</name>
        <name evidence="20" type="ORF">BRADI_3g51970v3</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">Bd21</strain>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Bd21</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK00812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570118.1">
        <property type="nucleotide sequence ID" value="XM_003570070.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ICQ0"/>
    <dbReference type="STRING" id="15368.I1ICQ0"/>
    <dbReference type="EnsemblPlants" id="KQK00812">
        <property type="protein sequence ID" value="KQK00812"/>
        <property type="gene ID" value="BRADI_3g51970v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100825822"/>
    <dbReference type="Gramene" id="KQK00812">
        <property type="protein sequence ID" value="KQK00812"/>
        <property type="gene ID" value="BRADI_3g51970v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100825822"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_37_4_1"/>
    <dbReference type="OMA" id="THGICHR"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009931">
        <property type="term" value="F:calcium-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000015">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000178">
        <property type="entry name" value="Calcium-dependent protein kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="98"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="399"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="435"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="471"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="507"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="28"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KQK00812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblPlants" id="KQK00812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="556" mass="61224" checksum="9AC547912A459B91" modified="2012-06-13" version="1">MGNTCGVTFRSMYFSSFRGASQRHDPEYAPIAAAAADDPPGKRPSRPAAAGPDGSLAAAAADDAPPPPASAMRRGALAPAELTANVLGHPTPSLHDHYLLGRKLGQGQFGTTYLCTHRATGVDYACKSIGKRKLITKEDVEDVRREIQIMHHLAGHRNVVAIKGAYEDQAYVHIVMELCAGGELFDRIIKRGHYSERKAAELTRIVVGVVEACHSLGVMHRDLKPENFLLANKDDDMSLKAIDFGLSVFFKPGQVFTDVVGSPYYVAPEVLRKSYGPEADVWTAGVILYILLSGVPPFWAETQQGIFDAVLKGTIDFDCDPWPVISESAKDLIRRMLNPHPAERLTAHEVLCHPWICDQGVAPDRPLDPAVLSRIKQFSAMNKLKKMALRVIAESLSEEEIAGLKEMFEAMDTDNSGAITYDELKEGMRKYGSTLKDTEIRDLMEAADVDNSGTIDYIEFIAATLHLNKLEREEHLVAAFSYFDKDGSGYITVDELQQACKEHNMPDAFLDDVIIEADQDNDGRIDYGEFVAMMTKGNMGVGRRTMRNSLNISMTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I1IF20</accession>
    <name>I1IF20_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100821112</name>
        <name evidence="8" type="ORF">BRADI_3g58720v3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probable histone demethylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01792.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQK01834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003570612.2">
        <property type="nucleotide sequence ID" value="XM_003570564.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IF20"/>
    <dbReference type="STRING" id="15368.I1IF20"/>
    <dbReference type="EnsemblPlants" id="KQK01792">
        <property type="protein sequence ID" value="KQK01792"/>
        <property type="gene ID" value="BRADI_3g58720v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK01834">
        <property type="protein sequence ID" value="KQK01834"/>
        <property type="gene ID" value="BRADI_3g58720v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100821112"/>
    <dbReference type="Gramene" id="KQK01792">
        <property type="protein sequence ID" value="KQK01792"/>
        <property type="gene ID" value="BRADI_3g58720v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK01834">
        <property type="protein sequence ID" value="KQK01834"/>
        <property type="gene ID" value="BRADI_3g58720v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100821112"/>
    <dbReference type="eggNOG" id="KOG0029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004498_5_0_1"/>
    <dbReference type="OMA" id="SSRGEMF"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IF20">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052901">
        <property type="term" value="F:spermine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046208">
        <property type="term" value="P:spermine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903602">
        <property type="term" value="P:thermospermine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000064">
        <property type="entry name" value="Lysine-specific histone demethylase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF381">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="SWIRM" evidence="7">
        <location>
            <begin position="135"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK01792.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK01834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="825" mass="88655" checksum="9EB3F9478D0B861F" modified="2012-06-13" version="1">MDMEEGNEAQPPPPPQEAPEAAEPVPMDQDEDQAAADPMEDEAAGGDAAAAVDPMEDDAPTSSPTPSAPSATAAVDDSTVARKRRRRKKQFPGMIPTAGVRVLRASSSSAATAAHLTGIPRRRGRPPTNSHVRQARELDSEALIALAAGFPADTLSEDEVAAVVIPTIGGTEQANYLVVRNHILALWRSNPLSPVSSNAALAAIRTEHAPLVAVAHSFLSDHAYINFGLAPSILSLPPSPPPSLPPPSVLIVGAGFAGLAAARHLMSLGFKVAIVEGRLRPGGRVFTKTMRSTAADYPDIAAAADLGGSVLTGINGNPLGVIARQLGFPLHKVRDKCPLYLPNGSEVNSDMDARVEAAFNQLLDKVCQLRQVVADSFPHGVDVSLGMALEAFRAAHGVAAEPEERMLLDWHLANLEYANAAPLADLSMAFWDQDDPYEMGGDHCFIPGGNSQFVRALADGIPIFYGQNVRRIQYGCDGVMVYTEKQTFRGDMALCTVPLGVLKKGDIDFVPELPAQKREAIQRLGFGLLNKVVILFPFDFWDGRIDTFGHLTEDSAQRGEFFLFYSYSSVSGGPLLVALVAGESAIEFEKKSPMENVEKVLETLRKIFSPKGIEVPNPLQAICTRWGTDRFTYGSYSYVAIGSSGDDYDILAESVADRIFFAGEATNRRYPATMHGALLSGYREAANIVRAARKRANKVDSPEKTDVNFEVKDTDKDDNIDLDDLFRTPDAAFGGFSVLHDPSTSEPDSTSLLRVGIGARKLGSGSLFLYGLIMRKNVAELAAMEGDEQRLSTLYRDFGTKLLGLDGLGHTGESLISRIKAASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I1IIE4</accession>
    <name>I1IIE4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100832793</name>
        <name evidence="5" type="ORF">BRADI_4g07340v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Bd21</strain>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LEA type SMP family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ86717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003575514.1">
        <property type="nucleotide sequence ID" value="XM_003575466.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IIE4"/>
    <dbReference type="STRING" id="15368.I1IIE4"/>
    <dbReference type="EnsemblPlants" id="KQJ86717">
        <property type="protein sequence ID" value="KQJ86717"/>
        <property type="gene ID" value="BRADI_4g07340v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832793"/>
    <dbReference type="Gramene" id="KQJ86717">
        <property type="protein sequence ID" value="KQJ86717"/>
        <property type="gene ID" value="BRADI_4g07340v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832793"/>
    <dbReference type="eggNOG" id="ENOG502QPSX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075678_0_0_1"/>
    <dbReference type="OMA" id="DHEHEMN"/>
    <dbReference type="OrthoDB" id="2014755at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042971">
        <property type="entry name" value="LEA_SMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007011">
        <property type="entry name" value="LEA_SMP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31174:SF20">
        <property type="entry name" value="OS06G0341300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31174">
        <property type="entry name" value="SEED MATURATION FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04927">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SMP" evidence="4">
        <location>
            <begin position="31"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="SMP" evidence="4">
        <location>
            <begin position="150"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="SMP" evidence="4">
        <location>
            <begin position="215"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ86717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ86717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="278" mass="28070" checksum="864F95273B8AD7D2" modified="2012-06-13" version="1">MAQAQPTRGDHEHEMNKGAAQEEQQPGPEPIQYGHVFAVTGDLAVQPIAPRDAAAMRSAEESVQGGAHAGGGGFRAATAMETAAAYNQAVGAVRPGQASHAAYGQGIAVTQTPVPGGCVVTEFVAGQVVGQYSVADQPMVMEEEDASSKVTIGEAMEAAALSAGDRPVDEADAQAIRAAEAQAQGAGKTMPGGLADQAQAAARANLRAQSDRDKIMVGDVLSDATTKLAGDRAAGREDAARVVEAETFADAGARTKASGVGAAVATAARLNQEDDHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IKY1</accession>
    <name>I1IKY1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BRADI_4g15720v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcriptional activator that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type IV zinc-finger family. Class C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IKY1"/>
    <dbReference type="SMR" id="I1IKY1"/>
    <dbReference type="EnsemblPlants" id="KQJ88128">
        <property type="protein sequence ID" value="KQJ88128"/>
        <property type="gene ID" value="BRADI_4g15720v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88128">
        <property type="protein sequence ID" value="KQJ88128"/>
        <property type="gene ID" value="BRADI_4g15720v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1043473_0_0_1"/>
    <dbReference type="InParanoid" id="I1IKY1"/>
    <dbReference type="OMA" id="MWANSRK"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045280">
        <property type="entry name" value="TIFY-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010399">
        <property type="entry name" value="Tify_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46125">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46125:SF23">
        <property type="entry name" value="OS11G0572901 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06200">
        <property type="entry name" value="tify"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51320">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Tify" evidence="14">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="13">
        <location>
            <begin position="193"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KQJ88128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="28584" checksum="0D5A9A46FF96457F" modified="2012-06-13" version="1">MQQQQPLPPICATAGADDEDPLALDFEFDVVLPDSDDDLFAFTPGFDQVFTDDQTAAAAAPMQQQQEMEVAEAEEQVGEEQLVMGYDGRMFVFDSVQPHKVETILSLLDGQELVPLPAQSTKPQLTYLVQPVVVPRDFDRPAALSRYRAKRQRKGLEPVVKADYSCRRDVALRMRRRGGRFVALSAKRTGDSPNELTLCTNCGERSDATPKMRRGPDGSRTFCNACGLMWDKTGRLRETAGNQSCGGGRWCRRSESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I1ILS3</accession>
    <name>I1ILS3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100822150</name>
        <name evidence="4" type="ORF">BRADI_4g19150v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Bd21</strain>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003576051.1">
        <property type="nucleotide sequence ID" value="XM_003576003.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ILS3"/>
    <dbReference type="EnsemblPlants" id="KQJ88532">
        <property type="protein sequence ID" value="KQJ88532"/>
        <property type="gene ID" value="BRADI_4g19150v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822150"/>
    <dbReference type="Gramene" id="KQJ88532">
        <property type="protein sequence ID" value="KQJ88532"/>
        <property type="gene ID" value="BRADI_4g19150v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100822150"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_0_6_1"/>
    <dbReference type="OMA" id="PSNHGVY"/>
    <dbReference type="OrthoDB" id="426361at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003670">
        <property type="entry name" value="Pentatricopeptide repeat protein PPR986-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000184">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF393">
        <property type="entry name" value="REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="180"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="285"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="320"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ88532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="50083" checksum="14F28CDD286A21C1" modified="2012-06-13" version="1">MPAPPLPGAGRRHMSLSLLADRCSTLRGLALVHAAMLVSGRLADDAFAASRLLNAYANLSPPAAVLRFLSSLPYAPNSFMLNTTLRALASSPDPASAFPFFSLHRRSGSSSYSPGRHTFPFLLKASARLALPVSQQIHALVVKHGLHLDTYVANGLVRAYSVAGLIGVARKVFDGLPERSTVVYTTMVSGYTLNGCYEDAMGAFGEMINEGFEPGDVVLASVLSACARSESGGLVIGRRVHNIIEMRGVEAPVGVILSTALVDMYAKNAAIEEAVTVFKGMPERRTATWNALISGLAHHGHGKYALVMFQQMLQEGVPPNATTFVGVLSAYCLSGMIDEARKAFRSMKDFGLTPSIQHYGCMVDLLGRSGLLVEAEEMIRGMTCEADTMIWGALLTACRNHGNIDIALRTALEILKLDPENHGVYVVLSNIYADAGRWQDVDRLRKVMKGALLSKIPGSSAVGGYGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I1INQ6</accession>
    <name>I1INQ6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100832179</name>
        <name evidence="2" type="ORF">BRADI_4g26250v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ89528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014758865.1">
        <property type="nucleotide sequence ID" value="XM_014903379.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1INQ6"/>
    <dbReference type="STRING" id="15368.I1INQ6"/>
    <dbReference type="EnsemblPlants" id="KQJ89528">
        <property type="protein sequence ID" value="KQJ89528"/>
        <property type="gene ID" value="BRADI_4g26250v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832179"/>
    <dbReference type="Gramene" id="KQJ89528">
        <property type="protein sequence ID" value="KQJ89528"/>
        <property type="gene ID" value="BRADI_4g26250v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832179"/>
    <dbReference type="eggNOG" id="ENOG502QTXB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013556_0_0_1"/>
    <dbReference type="OMA" id="CNNGVHY"/>
    <dbReference type="OrthoDB" id="1908091at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33870">
        <property type="entry name" value="CARDIOMYOPATHY-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33870:SF7">
        <property type="entry name" value="OS12G0127650 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ89528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ89528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="788" mass="86975" checksum="9DE724E4CC6A0CDF" modified="2012-06-13" version="1">MASDTNDLVLLRIRRLVRCSIRLGYRSACDHPGMLLSGVLLLFLYELCPPSVFAFLVSSSPVFALTALLLGVLLSYGESSVPCWDGGDVLEDHRILSLRSSISLAECSASAEVENVPFEVRLEERIENGKYNGDLNVREGSTDDRGCDPPCEEKMITHIAADDAVLHEEEAYVEEERTPNGKLHGTTCAEKYVSFVPDDTVLGAESCSKYPKSNATMESEERAEEIGEKAELQELDRSYIGTGNNGVHSQYQMGELMRACWQPVMRQDTPCYDSESDLSDDSSSPDASMTDIIPLLEELHPLIDLGTGHPSLAYKNNSHSSSDDDEEEEGVSSENSDEEGEEWQKDDGNDWKDVTGLNSLDMEENSKLENVMELRRTKNILKFELDKKLMDLQAADATRMLKDASSFHVQVPSISTSRQKTFDPSKDSEEMIELPQIPGSAPSLLPRRNLFDLQFDRTMDRRNRFEETWTPRSRFGSAAQNWKRRNSYGQQSSTYLQHHTSVTMENGEISGKHSCDDGHSDNDAEQEGNTGKLFGSLEAHLGEEMKILSAAISDVGVLGEVNHGTDEGSENASVRDNTSSLAGANDNSEPCVVEASSMSEVNSLFKCRMEEVLARSVSEPSVGQPLEVKPEGGLSVSLSSDSWMHASEASSVEVLNFQFARFDEEAATFAASALNNHPDESIRGRSSEALNGHRSGVPVELDGCCRVLLTEGDQKIADMSEERNELLNAGLLEEMNSPFEQLKEDGRSDMSMPRDCELEVDPVELNTGLLVIDADTDGDASEQRFRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1ITZ8</accession>
    <name>I1ITZ8_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100826915</name>
        <name evidence="5" type="ORF">BRADI_4g41400v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003578846.1">
        <property type="nucleotide sequence ID" value="XM_003578798.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1ITZ8"/>
    <dbReference type="FunCoup" id="I1ITZ8">
        <property type="interactions" value="2016"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1ITZ8"/>
    <dbReference type="EnsemblPlants" id="KQJ92046">
        <property type="protein sequence ID" value="KQJ92046"/>
        <property type="gene ID" value="BRADI_4g41400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100826915"/>
    <dbReference type="Gramene" id="KQJ92046">
        <property type="protein sequence ID" value="KQJ92046"/>
        <property type="gene ID" value="BRADI_4g41400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100826915"/>
    <dbReference type="eggNOG" id="KOG2394">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036858_0_0_1"/>
    <dbReference type="OMA" id="KVAFDPY"/>
    <dbReference type="OrthoDB" id="3367at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051362">
        <property type="entry name" value="WD_repeat_creC_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107:SF16">
        <property type="entry name" value="AT02583P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="310"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="546"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="547"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ92046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="567" mass="60195" checksum="CF6E3B2A110BFDDC" modified="2012-06-13" version="1">MAAASYSSSSSAAGLGGGGAAAAAVAGLKTYFKTPEGRYKLQYEKAHPAAVLHYNHGGKTVSQLTVAYLKERSTGQGSTPSTPSSSSGMRSAAARLLGNGNGSRALSFVGGNGVSRAVSGSSRIGGSLGTSASLGGSQPVANYDGKGAYIIFNAADTLFISDLNSQDKDPVKSMHFSNSNPLCHAFDPEAKEGHDLIIGMGSGDVYSMSLRQQLQESGRKPVAAQHYNKGDKDGTSISSRCTSVAWVPEREGIFVVSHSDGNLYVYDKCKDGNTECTFPAIKDPAQLIVSHAKSSKSNPIARWHICHGSINAMSFSPDGAYLATVGRDGYLRVFDFSKEQLIFGGKSYYGALLCCTWSSDGKYLLTGGEDDLVQVWSMDDRKIVAWGEGHNSWVSGVAFDPYWSPPNSDGTGENVVYRFGSVGQDTQLLLWDLAMDEIVVPLRHPSGGSPTFSGGSPSAHWDSACPPTGILQPSPRMRDVPKLSPLIAHRVHADPLSSLVFTNESILTICREGLIKIWSRPIRSENNQQSNSSELGIDNTVSKDRVITSSTKAGGSSFKQPSPVLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I1IV42</accession>
    <name>I1IV42_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BRADI_4g44678v3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="PNT65579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IV42"/>
    <dbReference type="EnsemblPlants" id="PNT65579">
        <property type="protein sequence ID" value="PNT65579"/>
        <property type="gene ID" value="BRADI_4g44678v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT65579">
        <property type="protein sequence ID" value="PNT65579"/>
        <property type="gene ID" value="BRADI_4g44678v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002706_15_6_1"/>
    <dbReference type="InParanoid" id="I1IV42"/>
    <dbReference type="OMA" id="WPAIWRS"/>
    <dbReference type="OrthoDB" id="733253at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002423">
        <property type="entry name" value="Os12g0109800 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001108">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000343">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At3g58590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000201">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015">
        <property type="entry name" value="OS07G0578800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015:SF1793">
        <property type="entry name" value="OS11G0109800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="248"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="349"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="4">
        <location>
            <begin position="554"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="PNT65579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="PNT65579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="735" mass="80246" checksum="38F7F1FAD5692A68" modified="2012-06-13" version="1">MPPSHLHAQLLKLGMSGDTFTANHLLIAYSRTGLLKNALGVFDEMPHRNLVSWTAMVSGSARGGASQLGLGLFVSMVRSGFLPNEFALASALRATASCHDGLRLGLSLHGVAVKVGVGGDPFAGSSLLLMYANHGRVADAEGAFAEVRRRDLACWNAMLEGYVSNGCGHEAMRAVVLMRRCDMFTYVSAVKACLITGESDFGRQLHGCVIHNMFEPDTSVMNVLVDMYFRAGLKDVAMAVFRRILRKDTISWNTVISGFAHDEDDREVLGCFADMSRSGCKPNEVTLSVMLRLCAAKENASLGLQIFGLAYCHGYSENVLVANAVINMLSRCGLLNSADGFFCNLRFRNIVTWNEMIAGYGLYSYSEDAMRLFRSMVCFGERPDEFTYSAVLSAFQEAHEPRNHEQVHASILKQGITSRQFVSTSLIKAKAVFGSVQSALKIMEDTGKMDLVSWGVVISAFLKHGLNNEVMSLFDLFRGDSMNRADEFILATVLNACANAALLRQCRRVHSLVIRTGHSNHFCVASALVDAYAKCGGIASAKSAFADVSSGSGDAILYNTMLTAYANHGLINEALSLYEDMARAQLVPTPATFVALVSACSNFGLVEQGKILFSLMLSEEHGIHPTRANYATLVDLLARKGFLHEAKDIIEVMPLHPWPSVWRSLMNGCRIHGNMELGVLAAEQILSMTPSSDGAYVSLSNVFADDGEWHLAEEARKMMAENQVRKVHAYSRIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I1IVV7</accession>
    <name>I1IVV7_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100832921</name>
        <name evidence="5" type="ORF">BRADI_5g02077v3</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580984.1">
        <property type="nucleotide sequence ID" value="XM_003580936.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IVV7"/>
    <dbReference type="EnsemblPlants" id="KQJ81653">
        <property type="protein sequence ID" value="KQJ81653"/>
        <property type="gene ID" value="BRADI_5g02077v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832921"/>
    <dbReference type="Gramene" id="KQJ81653">
        <property type="protein sequence ID" value="KQJ81653"/>
        <property type="gene ID" value="BRADI_5g02077v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832921"/>
    <dbReference type="eggNOG" id="ENOG502R5V6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015101_0_2_1"/>
    <dbReference type="OMA" id="NILNNMM"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IVV7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF59">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014095524" description="GDSL esterase/lipase" evidence="4">
        <location>
            <begin position="27"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KQJ81653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="39537" checksum="012A8F1B62A58DF7" modified="2012-06-13" version="1">MKKNCCIVKLVCVVVGLAATVCSSAAAAGQLVNAVYVFGDSLVDVGNNDYLPAPAPRARSPYGYDLPGRPTGRFTNGYNLADIISQRLGFEKSPLAYKSMLLHEKFLLVTCNIGANYASGGSGILDTTGNGTLTMSTQIKYFKRAVDKMVCLPSKEAMLSQSLFLLSAGGNDFSAFTGSINEAPAYIANLVSTYIKHIQSLYNLGARMVGILDVAPIGCTPGQRAGMPDGECNEVANSLARWFNSLFRIELAGTAAATMPELRYSIASNYNILTDMMANPLVAGIREVQTACCGAGKFMAEKMCGAEGTGVCADNHGEYMFWDMLHGTQATCERGALAVFYGAAPRYADPVNFSTLVTMKKKKKGSLVAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I1IX41</accession>
    <name>I1IX41_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100837441</name>
        <name evidence="3" type="ORF">BRADI_5g08180v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003579617.1">
        <property type="nucleotide sequence ID" value="XM_003579569.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IX41"/>
    <dbReference type="FunCoup" id="I1IX41">
        <property type="interactions" value="883"/>
    </dbReference>
    <dbReference type="STRING" id="15368.I1IX41"/>
    <dbReference type="EnsemblPlants" id="KQJ82280">
        <property type="protein sequence ID" value="KQJ82280"/>
        <property type="gene ID" value="BRADI_5g08180v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100837441"/>
    <dbReference type="Gramene" id="KQJ82280">
        <property type="protein sequence ID" value="KQJ82280"/>
        <property type="gene ID" value="BRADI_5g08180v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100837441"/>
    <dbReference type="eggNOG" id="ENOG502RY8Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130098_0_0_1"/>
    <dbReference type="OMA" id="TWQSPCA"/>
    <dbReference type="OrthoDB" id="532289at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034568">
        <property type="entry name" value="MED30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36406">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36406:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQJ82280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="18482" checksum="774F138F88672630" modified="2012-06-13" version="1">MASPAARQRQELAAEGQQHLEDTIAAAFQILSSMNDELCNPVLWSSSATASAAAAAGSQHPHQHQAAPPPPNCADSDVSDAAGGGAAGSGGSLDEARHRYKIAVAALRASISAVAPCAQELGSTEFKGDQAETEQLEERASALRKEIESKNKHLKLLIDQLRGLISDISMWQSPCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I1IX87</accession>
    <name>I1IX87_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="11 13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BRADI_5g08374v3</name>
        <name evidence="12" type="ORF">BRADI_5g08570v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="PNT60959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IX87"/>
    <dbReference type="STRING" id="15368.I1IX87"/>
    <dbReference type="EnsemblPlants" id="KQJ82361">
        <property type="protein sequence ID" value="KQJ82361"/>
        <property type="gene ID" value="BRADI_5g08374v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT60959">
        <property type="protein sequence ID" value="PNT60959"/>
        <property type="gene ID" value="BRADI_5g08570v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ82361">
        <property type="protein sequence ID" value="KQJ82361"/>
        <property type="gene ID" value="BRADI_5g08374v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT60959">
        <property type="protein sequence ID" value="PNT60959"/>
        <property type="gene ID" value="BRADI_5g08570v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832834"/>
    <dbReference type="KEGG" id="bdi:106865484"/>
    <dbReference type="eggNOG" id="KOG0156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_4_0_1"/>
    <dbReference type="OMA" id="EDIRHYA"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20618">
        <property type="entry name" value="CYP71_clan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000038">
        <property type="entry name" value="Cytochrome P450 84A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944:SF7">
        <property type="entry name" value="OS09G0264400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="7 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KQJ82361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="PNT60959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="546" mass="60144" checksum="1CC517B9D1DBBD2A" modified="2012-06-13" version="1">MELALPAAWASSFLAVLMATAILLLVLRHGRNSHRHRYRLPPGPKPWPIIGNLHLLGALPHRSLRELSKRYGPLIQLRLGSFPVVVGSSAETARFFLKTHDAASAGRPRTAAGRHTAYNYSDMLWSPYGAHWRRLRRVCLAELFSAARLGSFEHIRRDEVRALLRGLHDASGIGMPVVVKVKEHLFAATLGMISRMVLGNKYVGKDAGSGSAMEPEEFMRVMEEFFFLNGALNVGDFVPWLGWLDLQGYVGRMKNVAWALDAFMERVLDEHDARRRLEGDGSFVAKDMADVLRLQQRRQIESGDEEGEPLERDSVKALAMDLIAGGTNTNAVTLEWAMSELLRNPAAMDAAGEELGRVVGRGRLVREDDIQSLPYLRAVVKETLRLHPVGTLLAPHEAQEDATVPAFVSGNGVSYDVPAGTRVLVNVWAIARDPALWGPKPEEFRPERFLEGGGNSGVDVVGQDMELLPFGAGRRMCPGYGLGIKVVQICLANLIHGFAWRLPDGVAAEELGMDEVFGLTTSRKFPLEAVLEPKLPAHLYTLHSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I1IXD7</accession>
    <name>I1IXD7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100828249</name>
        <name evidence="2" type="ORF">BRADI_5g08960v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ82438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010239855.1">
        <property type="nucleotide sequence ID" value="XM_010241553.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IXD7"/>
    <dbReference type="STRING" id="15368.I1IXD7"/>
    <dbReference type="ESTHER" id="bradi-i1ixd7">
        <property type="family name" value="Duf_1350"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ82438">
        <property type="protein sequence ID" value="KQJ82438"/>
        <property type="gene ID" value="BRADI_5g08960v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100828249"/>
    <dbReference type="Gramene" id="KQJ82438">
        <property type="protein sequence ID" value="KQJ82438"/>
        <property type="gene ID" value="BRADI_5g08960v3"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSXN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047079_1_1_1"/>
    <dbReference type="OMA" id="WIESNSG"/>
    <dbReference type="OrthoDB" id="4892at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I1IXD7">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010765">
        <property type="entry name" value="DUF1350"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34127">
        <property type="entry name" value="OS04G0405600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34127:SF1">
        <property type="entry name" value="OS04G0405600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07082">
        <property type="entry name" value="DUF1350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ82438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="452" mass="48611" checksum="E50E53E8E47BD4A2" modified="2012-06-13" version="1">MIRVASPRPPSAVQLRGCGGASSSALPYRGSGSCFAATNGSRRTRNRRSAISCSSSEDSEGPRGAAPPTPAPAPTAPSEGSIQLYSQIERVITEAAKQSSEGWGSTGDWTEIEGAWVLRPKSSEPSFVVHFIGGIFVGAAPQVTYRFFLERLADKGALVIATPYASGFDHFFIADEVQFKFDRCLRNLVEPVNDLPTFGVGHSLGSVIHLLIGSRYAVQRNGNILMAFNNKEASQAVPLFSPVIVPMAQSFGPILSQLTSSPTIRFGQAEAAIKQLENLGPPVVKQLLPLIQQLPPLYMDLAKGREEFIPKPEETRRLIKSYYGISRNLLIKFKDDQIDETSILAQVLSSESSISSLLDMSIRSLPGDHGMPLQQVLPDVPPGMADAVNRGGELLANLTTGTPWEAVAKELGTTFGTDSGILRTQIPEDVNALVDVIISWIASNSGPKLLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I1IZ76</accession>
    <name>I1IZ76_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC112268533</name>
        <name evidence="7" type="ORF">BRADI_5g14400v3</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ83346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311222.1">
        <property type="nucleotide sequence ID" value="XM_024455454.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024311223.1">
        <property type="nucleotide sequence ID" value="XM_024455455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1IZ76"/>
    <dbReference type="FunCoup" id="I1IZ76">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ83346">
        <property type="protein sequence ID" value="KQJ83346"/>
        <property type="gene ID" value="BRADI_5g14400v3"/>
    </dbReference>
    <dbReference type="GeneID" id="112268533"/>
    <dbReference type="Gramene" id="KQJ83346">
        <property type="protein sequence ID" value="KQJ83346"/>
        <property type="gene ID" value="BRADI_5g14400v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:112268533"/>
    <dbReference type="eggNOG" id="KOG1237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009313_4_0_1"/>
    <dbReference type="OMA" id="CKEAHGW"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000331">
        <property type="entry name" value="Protein NRT1/ PTR FAMILY 4.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KQJ83346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ83346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="61648" checksum="68E02EDD7AD0FDFA" modified="2012-06-13" version="1">MALGGFVDWRGNTINREVHGGVRAAWFLYVLTVVTNVVIVPNLLNMVTYLQGTMHMGVSGSATTATNFFGATSGFALIGAFLSDSYITRSRTVLIFGPFMFLGYGLLALQAYLPSLHPPPCNIEAELNNCKKVHGWNAALLYTGLYMSAFGDGSIRVCLPSLGADQFDHEDPTESHQQSSFFNWYTFGISFGGFVGLILIAWLENFKGWDIGLGVCCILILLGLVIVASGLPFYRNQVPEGSPLTRILQVLVVAFRNRKHELPQKLEETQESNSRTGSVGAFSQTNSLKFLDKACISRGKSGAWSVCTATKVEETKIVLLMLPVFITSMIGYISNIILLTFTVQQGGMTNTRLGMIHVAPATLFIIPITFQMAMLAVYDQFLVPFLRRRTGYVRGITHLQRIGIGFGSMSLASAIAAIVERKRKQAVVQMSLFWLAPQFFLLGVSDVTSFTGLLEFFNSEAPRGMKSIATALFWCELGLASLMATFLVEIVNKATRHGNQGGWLEGSSLNSSHLDLFYWVVAVVGLLSFFNYLYWAKKYVYQHNPRITEPMVDHQDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-06-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I1J186</accession>
    <name>I1J186_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100832103</name>
        <name evidence="10" type="ORF">BRADI_5g20130v3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ84327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003580443.1">
        <property type="nucleotide sequence ID" value="XM_003580395.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I1J186"/>
    <dbReference type="FunCoup" id="I1J186">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQJ84327">
        <property type="protein sequence ID" value="KQJ84327"/>
        <property type="gene ID" value="BRADI_5g20130v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832103"/>
    <dbReference type="Gramene" id="KQJ84327">
        <property type="protein sequence ID" value="KQJ84327"/>
        <property type="gene ID" value="BRADI_5g20130v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832103"/>
    <dbReference type="eggNOG" id="KOG0048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028567_19_0_1"/>
    <dbReference type="OMA" id="IIKLHAM"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000310">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641:SF1103">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="64"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="64"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="118"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQJ84327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="365" mass="39380" checksum="8F45D48F6333250F" modified="2012-06-13" version="1">MGKGRAPCCAKVGLNKGSWTPEEDMRLIAYIQKYGHANWRALPKQAGLLRCGKSCRLRWINYLRPDLKRGNFTAEEEETLIKLHAMLGNKWSKIAACLPGRTDNEIKNVWNTHLKKRVAAASGGEKKNKKNTKNNKESGAKRQKKPDLPPAPSPSQSSSTTTTTTTTNCSSGDSAGEQQSNTSKETDALDNIMEIPTLDALGFDFDMLVQDTDPVPAQTSYCVPPPAAAASPCSSASPPCAADGELLDLPELDIVPELWSIIDGDAEAPWSNAAAACHVEAGEDGKEWWLEDLERELGLWGPMEDYQCINPGPQAQSGRVDPLSVSAGVELDPVSCYFQAGPAVATSALQGSEHSAVLTDNRMDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E1E5</accession>
    <name>A0A0Q3E1E5_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BRADI_5g01189v3</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000884">
        <property type="protein sequence ID" value="KQJ81510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3E1E5"/>
    <dbReference type="STRING" id="15368.A0A0Q3E1E5"/>
    <dbReference type="EnsemblPlants" id="KQJ81510">
        <property type="protein sequence ID" value="KQJ81510"/>
        <property type="gene ID" value="BRADI_5g01189v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ81510">
        <property type="protein sequence ID" value="KQJ81510"/>
        <property type="gene ID" value="BRADI_5g01189v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3E1E5"/>
    <dbReference type="OrthoDB" id="308440at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053307">
        <property type="entry name" value="Mitochondrial_IM_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47040:SF1">
        <property type="entry name" value="MITOCHONDRIAL ATP-INDEPENDENT INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47040">
        <property type="entry name" value="OSJNBA0068L06.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ81510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="183" mass="19962" checksum="A78BD83B1BDFB40F" modified="2016-01-20" version="1">MVSLSTWFRYAAHKFEYSISLSWKKYNVGQINSTELTDAIWKSFFQGKLTFAHWTKDEGFPPLPQTTSFKLGGGGRAGWVDRGRRRSAVRREECGGVLREVRRGRRRGLGGGGGPGWLDGSGMLLGGRNAAAAQAGWSTAAACWRRERGAATRTESRAPVRREGAEGSCALCLCDEEAASLCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E2T3</accession>
    <name>A0A0Q3E2T3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribulose bisphosphate carboxylase large chain</fullName>
            <ecNumber evidence="3">4.1.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BRADI_5g05663v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 (2R)-3-phosphoglycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="4.1.1.39"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>D-ribulose 1,5-bisphosphate + O2 = 2-phosphoglycolate + (2R)-3-phosphoglycerate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36631"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the RuBisCO large chain family. Type I subfamily.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:reductive pentose-phosphate cycle"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RuBisCO_lsu_fd_N"/>
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        <property type="entry name" value="RuBisCO_lsu_N_sf"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
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        <location>
            <begin position="24"/>
            <end position="121"/>
        </location>
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        <location>
            <position position="121"/>
        </location>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023238"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF02788"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQJ82087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="121" mass="13351" checksum="0B17BD9BB1F50A19" modified="2016-01-20" version="1" fragment="single">MSPQTETKASVGFKAGVKDYRLTYYTPEYETKDTDILVAFRVSPQPGVPPEEVGAAVAAESSIGTWTTVWTDGLTSLDCYKGRCYDIEPVPGEDSQWICYVAYPLDLFEEGSVTNMFTSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E449</accession>
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        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">BRADI_5g10033v3</name>
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    <organism evidence="1">
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            <taxon>Poaceae</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">cv. Bd21</strain>
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    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ82607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ82607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="77" mass="8423" checksum="5C40FAD08A119043" modified="2016-01-20" version="1">MAGWSLELSSQRERKAGTGSLFAASLRNKILHSTLQCAAHVQDYIRPLTEFSVGEQVVRATCKLKAKSCISQLACRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3E6S1</accession>
    <name>A0A0Q3E6S1_BRADI</name>
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    </protein>
    <gene>
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    <organism evidence="1">
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
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                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
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                <person name="Mockler T.C."/>
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                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="gene ID" value="BRADI_5g03836v3"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ81924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ81924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-06-28" version="12" xmlns="https://uniprot.org/uniprot">
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    <name>A0A0Q3EDU7_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="1 2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="1">
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        <lineage>
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    <reference evidence="1 2" key="1">
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            <dbReference type="PubMed" id="20148030"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQJ84628">
        <property type="protein sequence ID" value="KQJ84628"/>
        <property type="gene ID" value="BRADI_5g21925v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ84628">
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        <property type="gene ID" value="BRADI_5g21925v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3EDU7"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQJ84628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ84628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="69" mass="8013" checksum="17B5B68BC039DC00" modified="2016-01-20" version="1">MLMQAQHLRTSTLRDQLCADPSRDLFSSGFRHYDQGSSGEYLDSAPLHWRTAWIYIAQRRQEIANSLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3ELU4</accession>
    <name>A0A0Q3ELU4_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DET1- and DDB1-associated protein 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100822373</name>
        <name evidence="4" type="ORF">BRADI_4g19110v3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3ELU4"/>
    <dbReference type="FunCoup" id="A0A0Q3ELU4">
        <property type="interactions" value="937"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3ELU4"/>
    <dbReference type="EnsemblPlants" id="KQJ88527">
        <property type="protein sequence ID" value="KQJ88527"/>
        <property type="gene ID" value="BRADI_4g19110v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQJ88527">
        <property type="protein sequence ID" value="KQJ88527"/>
        <property type="gene ID" value="BRADI_4g19110v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="445357at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3ELU4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033575">
        <property type="entry name" value="DDA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018276">
        <property type="entry name" value="DDA1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31879">
        <property type="entry name" value="DET1- AND DDB1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31879:SF2">
        <property type="entry name" value="DET1- AND DDB1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10172">
        <property type="entry name" value="DDA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DET1- and DDB1-associated protein 1" evidence="3">
        <location>
            <begin position="28"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ88527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="179" mass="19265" checksum="8F16909F982BCA75" modified="2016-01-20" version="1">MAAEGSGGGGGGRKTSSPHKTKERGAAKFLAGLPSHGNFSSSPISSNLGGLRVYVCEHDTDPPAGQVIETDTTNILIRHLQLKKKESEAKDSRTPGENGKGKRSAARSLDGQHSSKKPNLSTSGLPSSGWQPNKVPEAFRETPLARQRRLWAGTKQLESWRHTEQGISIIQFSTEAHFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3F261</accession>
    <name>A0A0Q3F261_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100839766</name>
        <name evidence="2" type="ORF">BRADI_4g45022v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Bd21</strain>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ92604.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_024318929.1">
        <property type="nucleotide sequence ID" value="XM_024463161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3F261"/>
    <dbReference type="STRING" id="15368.A0A0Q3F261"/>
    <dbReference type="EnsemblPlants" id="KQJ92604">
        <property type="protein sequence ID" value="KQJ92604"/>
        <property type="gene ID" value="BRADI_4g45022v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100839766"/>
    <dbReference type="Gramene" id="KQJ92604">
        <property type="protein sequence ID" value="KQJ92604"/>
        <property type="gene ID" value="BRADI_4g45022v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="684590at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3F261">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37258">
        <property type="entry name" value="FANTOM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37258:SF1">
        <property type="entry name" value="FANTOM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQJ92604.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ92604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="326" mass="34465" checksum="ED0A24B9AC9E1397" modified="2018-03-28" version="2">MPCCSWVLSIVASSSGGAGTPSWLHRLHAKGGLSFPKDLQIDDLLYGGSRSPAQPAAAAAAPHPPPAAPLPPPPTPAATPQAPPPKPNPTRTTPTRPNPSAATNTDKPALPQQPPTPPPPPLSGVVSDIFAFAVPSAPRSTRLLKPFRKQSRPRPCSDDEGDDKTPRSSTKENKNKKETKTKGRKRRRAERDAALAAAANGTVERNSKTDVTVIDSSTEGWKGSKVLHIRGLSWKVQNKKVSLVTEPETLAKGKRRAGLVAKITRDREKERKAAASQGNIAISGDLVKEPDGATEMLKRPRSSEPAPGGRTAAAFLLPSTSTLSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FSF1</accession>
    <name>A0A0Q3FSF1_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histone deacetylase interacting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100824976</name>
        <name evidence="10" type="ORF">BRADI_2g00860v3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Bd21</strain>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Bd21</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK02336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003565696.1">
        <property type="nucleotide sequence ID" value="XM_003565648.4"/>
    </dbReference>
    <dbReference type="STRING" id="15368.A0A0Q3FSF1"/>
    <dbReference type="EnsemblPlants" id="KQK02336">
        <property type="protein sequence ID" value="KQK02336"/>
        <property type="gene ID" value="BRADI_2g00860v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100824976"/>
    <dbReference type="Gramene" id="KQK02336">
        <property type="protein sequence ID" value="KQK02336"/>
        <property type="gene ID" value="BRADI_2g00860v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100824976"/>
    <dbReference type="OrthoDB" id="10265969at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FSF1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000003">
        <property type="entry name" value="Paired amphipathic helix SIN3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="9">
        <location>
            <begin position="501"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="223"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="440"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="800"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="884"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1007"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="250"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="441"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="800"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="884"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="917"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="952"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1039"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1057"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1093"/>
            <end position="1102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KQK02336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblPlants" id="KQK02336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="1439" mass="161566" checksum="A1ECC099A11D886A" modified="2016-01-20" version="1">MKRAREEALVGSQQQQQQQLKRPNPARSDPTAPPQAMPVSGSASAAAAPPPQAAAAAPAQPSSSLAAGAGQKLTTNDALVYLKAVKDKFQDNRAKYEEFLEVMRDFKSERIDTNGVIIRVKTLFNGYPELILGFNTFLPKGFAIRLQEEKKPVDFMEAINFVNKIKTRFQRDEHVYKSFLDILNMYRKDNKSIQDVYQEVAVLFSDHKDLLEEFQHFLPDTSVSPQAVTAPRGALVNRDDKTTVMPPVSRNEKPRAYPSHADRDFTVDRPDVEHDRQRRQKEKERKAERDKRDYERDEKDGEHDSKEPDMGQRKRRPFTSANPTGAETHQGGFPENHGINTASASSYDNNDVLKSVYPQEFQFCDKVKEKLEHDAYQEFLKCVHIYSQEIITRSELKNLVSDILQHYPDLMNGFNEFLEHCENIDGFLAGVFSKKQPGRLVKTEDKERDKEHEREDRDRDRDKEREKGRERLNPKEGPSQKPSIIKEKYLCKPISELDLSNCQRCTPSYRLLPKNYPMPPASNRTDLGASVLNDHWVSVTSGSEDYSFKHMRKNQYEESLFRCEDDRFELDMLLESVNAATKRVEELIEKMQDNSVKPDSPIRIDEHLTPLNLRCVERLYGDHGLDVMDVLRRNASVALPVILTRLKQKQEEWSRCRSDFNKVWAEIYAKNYHKSLDHRSFYFKQQDSKNLSTKSLLTEIKEINEKKRKEDDVLIAIAAGNRRPIVPNMSFEFVDLNIHEDLYKIVKYSCGEVCSSSDQLDKVMRIWTTFMEPILGVPPRSNGTVDVDPIKPKNGITKSSIVTVGESNSAPAGTATKQGHGDESMPQEQAPSTVVRLVKGVAADSQNGFHDADRTARAGGELPNAALNGRVQAGASATVEISAVNTQNMSTERSAENVSVPRTEQHSHIKGNLDTTSGANASRSSHAGAGSAAGPRAGKEALPSVEGGETGRSVSSLNGGSTSEGNKGGLFNEATASHNISKIEREEGELSPNGDFEEDNFVPLEDGAAEGTSKTKEGSTSRTFKAGTGEVGPFAEAAGENDADDEGEESTQRSSEDSENASEAGEDASGSESGDGEQCSREDHDEEEEDMDHDDHDAKAESEGEAEGTTETHDVEGGMSLPVSERLLYSVKPLAKHVPTSLLDREDKSSHIFYGNDSFYVLFRLHQILYERLLSAKTNSSSAEKKWRTSKDTNPPDLYAKFIDALYNLLDGSSDNTKFEDDCRAIIGTQSYVLFTLDKLIYKVVKQLQAIATDEMDNKLLQLYLYEKSRSPGRFFDLVYHENARVLLHDESMYRFECCSNPTRLSIQLMEYGHEKPEVTAVSIEPNFSSYLFSEYLSSSSDTMLSDGVFLGRNKRKHSNKDEPSDSLKTMDGIKVANGLECKISCKTSKVSYVLDTEDFLFRLRKRRLPPGENVPDKSLTSKQNAAKLQRFHMFLSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3FYI3</accession>
    <name>A0A0Q3FYI3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BRADI_2g07597v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03397.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3FYI3"/>
    <dbReference type="FunCoup" id="A0A0Q3FYI3">
        <property type="interactions" value="397"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK03397">
        <property type="protein sequence ID" value="KQK03397"/>
        <property type="gene ID" value="BRADI_2g07597v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK03397">
        <property type="protein sequence ID" value="KQK03397"/>
        <property type="gene ID" value="BRADI_2g07597v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3FYI3"/>
    <dbReference type="OrthoDB" id="781802at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3FYI3">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000043">
        <property type="entry name" value="Cytochrome P450 99A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF15">
        <property type="entry name" value="CYTOCHROME P450 71A2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 10" id="KW-0349">Heme</keyword>
    <keyword evidence="7 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK03397.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK03397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="57069" checksum="893DF2450B9185B6" modified="2018-03-28" version="2">MAVSTLALVILVPALVVSLFYLFRSAKRSPAPPQDGRQLLPPSPGRGLPLMGHLHLLGSLPHRSLRALAAAHGPVMLLRLGRVPAVVVSSAAAAEEVMRARDLAFASRPRSAVAERLLYGRDVAFAPYGEYWRQARRICVVHLLNTRRTLSFRRVREEEAAALVHRVRYAASGVDMCELIVAYANTVVSRAAFGDESARGLYRDGGRGRELRLVFGDFVQLLGTAPWGSSCRVWAGWTPGVEGRIRRTFEALDGVLEKVIDDHRRRRQPAGARHKLGDDGVDDHKDFVDVLLDVNETDGEAGVRLDTTEIKAIILDMFAAGTDTTSTAIEWAIAELITHPDDMQKLQDEIRATVGAAGRVTEDHLDKLRYLNAVVKETLRLHPPIPLLVPREPPNDAEILGYHVLARTRVIINAWAIGHDPATWERAEEFLPERFLLDKAHVDYRGQDFGLVPFGAGRRGCPGIEFAAPTVKMALASLLYHFDWETAGGTSLDMRERNGLSVHLKSGLPLVAKPRFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GBM9</accession>
    <name>A0A0Q3GBM9_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100832324</name>
        <name evidence="8" type="ORF">BRADI_2g38510v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK07941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010231825.1">
        <property type="nucleotide sequence ID" value="XM_010233523.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GBM9"/>
    <dbReference type="EnsemblPlants" id="KQK07941">
        <property type="protein sequence ID" value="KQK07941"/>
        <property type="gene ID" value="BRADI_2g38510v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100832324"/>
    <dbReference type="Gramene" id="KQK07941">
        <property type="protein sequence ID" value="KQK07941"/>
        <property type="gene ID" value="BRADI_2g38510v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100832324"/>
    <dbReference type="OrthoDB" id="624649at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GBM9">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:protein kinase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000465">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2700">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056284">
        <property type="entry name" value="AIR9-like_A9"/>
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    <dbReference type="InterPro" id="IPR055474">
        <property type="entry name" value="DUF7046"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707">
        <property type="entry name" value="C2 CALCIUM/LIPID-BINDING PLANT PHOSPHORIBOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45707:SF76">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23080">
        <property type="entry name" value="DUF7046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23197">
        <property type="entry name" value="IG_AIR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="53"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK07941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK07941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="701" mass="79069" checksum="0DC1320413013132" modified="2016-01-20" version="1">MASPSSNSDGDLQEGLRPCNSNPISSRDTDMDKKLQNAASLTFRLLQEITDNFSEGRKIGQGAYGTVYKGVYDNGEEIAVKLLHNNMQGTDDGQFKREFENLMRLEHQNVVRLVGYCYETQHKPMLHEGETIFADEISRALCFEYMPNGSLTKHISEECDGLDWNTRYKIIKGACEGLKYLHEGFKEPIYHMDLKPDNILLDENMMPKLADFGLSKLYDGEQTMITQTLMGTVGYLPPEYLFEHIVSKKLDIFSLGVVITKIIAGPRGPSRRAEMTHQEFLDQVHENWRKRLQETWRASRTLEAYCEQVNTCIEIALGCMETDRHNRPTIVHIIHQLNKTETVIKELKNDQGAEPSGDEYAAADPQPLPIPSIYGLRITGEAFPGGKLQASGYSISGTTSVVFQWVRHLEDGSVYNIEDAACPTYLVTGDDVGTLLAVEVQPLVDQERKGETVKVYANEKRKIMWENDSLPSIDGLRITGEAFPGRELTANGYSINGTTLCNFQWVHHLEDGSVRFIKDSTNPTHLVTDDDVDTILAVEVQPLNEWGRKGEIVKVYANEQRKITDDPEMKELIKTSLSIGHASYEVLLPVRYLDMWEPAVLAIKREGYSIKCNGQRGVVITEKFQQATAINIPYGRPTEFSIQSADGAEYNLKPAENSPSRDFIVQILRSFRMQAVQNGKGRRKGNFFMQSRVERGESVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GC41</accession>
    <name>A0A0Q3GC41_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100836457</name>
        <name evidence="12" type="ORF">BRADI_2g43950v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLAC1 S-type anion channel family.</text>
    </comment>
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        <property type="protein sequence ID" value="KQK08799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KQK08800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003569340.4"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010233740.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_014898373.2"/>
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        <property type="nucleotide sequence ID" value="XM_024460322.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_024460323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3GC41"/>
    <dbReference type="STRING" id="15368.A0A0Q3GC41"/>
    <dbReference type="EnsemblPlants" id="KQK08799">
        <property type="protein sequence ID" value="KQK08799"/>
        <property type="gene ID" value="BRADI_2g43950v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK08800">
        <property type="protein sequence ID" value="KQK08800"/>
        <property type="gene ID" value="BRADI_2g43950v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="KQK08801">
        <property type="protein sequence ID" value="KQK08801"/>
        <property type="gene ID" value="BRADI_2g43950v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT72420">
        <property type="protein sequence ID" value="PNT72420"/>
        <property type="gene ID" value="BRADI_2g43950v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100836457"/>
    <dbReference type="Gramene" id="KQK08799">
        <property type="protein sequence ID" value="KQK08799"/>
        <property type="gene ID" value="BRADI_2g43950v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08800">
        <property type="protein sequence ID" value="KQK08800"/>
        <property type="gene ID" value="BRADI_2g43950v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08801">
        <property type="protein sequence ID" value="KQK08801"/>
        <property type="gene ID" value="BRADI_2g43950v3"/>
    </dbReference>
    <dbReference type="Gramene" id="PNT72420">
        <property type="protein sequence ID" value="PNT72420"/>
        <property type="gene ID" value="BRADI_2g43950v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100836457"/>
    <dbReference type="OrthoDB" id="1099at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3GC41">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09323">
        <property type="entry name" value="TDT_SLAC1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030183">
        <property type="entry name" value="SLAC/SLAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31269">
        <property type="entry name" value="S-TYPE ANION CHANNEL SLAH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31269:SF2">
        <property type="entry name" value="S-TYPE ANION CHANNEL SLAH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="543"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="193"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="604"/>
            <end position="634"/>
        </location>
    </feature>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="604"/>
            <end position="628"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblPlants" id="KQK08799"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2023-09-13" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GKG1</accession>
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            <fullName evidence="1 2">Uncharacterized protein</fullName>
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        <name evidence="1" type="ORF">BRADI_2g57015v3</name>
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        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Bd21</strain>
        </source>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="1">Bd21</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KQK10909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="93" mass="10685" checksum="B5405E4666D65E04" modified="2016-01-20" version="1">MAVQNSHVYRSSYKKSARMKLQYLNRTYVSSLATKSGSSTGDFFYKINQLRHTVYVAQREVAKQVKLSKPLAARFICKHSSILGTFFAERCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3GVV6</accession>
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            <fullName evidence="1 2">Uncharacterized protein</fullName>
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
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        </source>
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    <dbReference type="Proteomes" id="UP000008810">
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    </dbReference>
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        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="KQK15095.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK15095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="113" mass="12997" checksum="868C6887AE7105FC" modified="2018-03-28" version="2">MFDDASFMSMMADEEMNHVEPSQDDECFVDPSPMKGRQMYYTTEEDDNSVLAWEAITLDAVNGVEQSASTYWQSVDHRIQKTPPPPRQKTQISLTNRWSGKQDVCNKWVCALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H179</accession>
    <name>A0A0Q3H179_BRADI</name>
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        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
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    </protein>
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    <organism evidence="1">
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        <lineage>
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            <taxon>Poaceae</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQK16389">
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        <property type="gene ID" value="BRADI_1g28525v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16389">
        <property type="protein sequence ID" value="KQK16389"/>
        <property type="gene ID" value="BRADI_1g28525v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="544346at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H179">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF19">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L domain-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="KQK16389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="14399" checksum="BB7B66EAA23EE3FE" modified="2016-01-20" version="1">MHALASSFASGHVQGGDPCLPSPWSWVQCTASKPQPRVVSIDLSGKNLMGSIPPELAALPCLAQIRLDNNMLTGAIPDLSAASNLSIIKFERQALCNCASFHILSAVLTVVSKRLATCILSFNSICPFMLSAFNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H3K5</accession>
    <name>A0A0Q3H3K5_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CRM-domain containing factor CFM3, chloroplastic/mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Protein CRM FAMILY MEMBER 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100822894</name>
        <name evidence="18" type="ORF">BRADI_4g14900v3</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Bd21</strain>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Bd21</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Binds specific group II introns in chloroplasts and facilitates their splicing. Acts on subgroup IIB introns. The substrates of the subgroup IIB also require the CRM domain proteins CAF1 or CAF2, with a simultaneous binding of CFM3 and CAF1 or CAF2. May influence the biogenesis of the mitochondrial small ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with RNA. Part of large ribonucleo-protein particles that contain CAF1 and/or CAF2, and RNC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ88010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003577404.2">
        <property type="nucleotide sequence ID" value="XM_003577356.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H3K5"/>
    <dbReference type="STRING" id="15368.A0A0Q3H3K5"/>
    <dbReference type="EnsemblPlants" id="KQJ88010">
        <property type="protein sequence ID" value="KQJ88010"/>
        <property type="gene ID" value="BRADI_4g14900v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100822894"/>
    <dbReference type="Gramene" id="KQJ88010">
        <property type="protein sequence ID" value="KQJ88010"/>
        <property type="gene ID" value="BRADI_4g14900v3"/>
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    <dbReference type="KEGG" id="bdi:100822894"/>
    <dbReference type="OrthoDB" id="551352at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H3K5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000373">
        <property type="term" value="P:Group II intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblPlants"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000003">
        <property type="entry name" value="CRM-domain containing factor CFM3B, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000002">
        <property type="entry name" value="CRS2-associated factor 1, chloroplastic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045278">
        <property type="entry name" value="CRS1/CFM2/CFM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846:SF19">
        <property type="entry name" value="CRM-DOMAIN CONTAINING FACTOR CFM3A, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846">
        <property type="entry name" value="CRS1 / YHBY (CRM) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CRM" evidence="17">
        <location>
            <begin position="173"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="17">
        <location>
            <begin position="377"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="17">
        <location>
            <begin position="589"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="771"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="709"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="793"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KQJ88010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ88010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="886" mass="99187" checksum="BCD3CE966B4FABF9" modified="2016-01-20" version="1">MATAAMAFSPALHHPPRLRHLLLSSISLSSSSCPYPWLSAWSRPRRGLRPPAPALDLRPEPSPTSDSDEEDSVGTSRHAGRSTMSLILRRLQRAGYSPEPQAAHAATASRHPQRGSVEDVFRADDGVLPNARGGFDDDAESSLVDARFPWERPMPPPEAAARATKSPVWMAELTLPEAELRRLRHATMRIKSRIQVGGAGVTREIVAKIKEKWKTDEVVRVKVSGTPALNMRLFHEILERKTGGLVIWRSGTSVSLYRGVAYDVPETTKGTNRNWQALGMKSSINIPPMPSSLPNEKVNGMQDRVGALVAVTENEETAETVPEIKYEEEIDRLLDELGPRYSDWPGSNPLPVDADLLPATVPGYKPPFRVLPYGVRRSLSRKDTTNLRRLGRGLPPHFALGRSRQLQGLAAAMVKLWERSSIAKIALKRGVQLTTSERMAEDLKKLTGGVMLSRNNDFVVFYRGKDFLSSELAEVLLERERSMKSLQDEEQARLDRTPSFASSTEAFIEPSVAGTLEETLEANSKYGNKVDENHMDKMTKTVEAAKHADVVRKLEWKLSLAEKRIAKAERVLGKVETALKPSEDTNPHETITEEERFMFRKLGLRMKAFLLLGRRGVFDGTIENMHLHWKYRELVKILVKVKSFTEVKRIALSLEVESGGILVSVDKVSKGYAIVVFRGKDYRRPSMLRPRNLLSKRKALARSIEIQRMQALNRHIGKLNRRVNQLRSELVQIEGAKDQGDVELYAKLDSAYSSEDEDVEDEDDEAYLRSFDSAVGRDTTDDGNETILHDSDSSYEDNGDYAEEDEDDEYEEDAEGSDYEDYDNVVENSEPSVISGGYMDSDYSARDCNRLEGGNEPDGGSSDADDSRNSSPYRHRDQPAKADQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H630</accession>
    <name>A0A0Q3H630_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G patch domain-containing protein TGH homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein TOUGH homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100831112</name>
        <name evidence="12" type="ORF">BRADI_1g42320v3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Bd21</strain>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Bd21</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of microRNA (miRNA) and small interfering RNA (siRNA) biogenesis. May assist Dicer-like (DCL) proteins to efficiently process and/or recruit the precursors of miRNAs and siRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014753022.1">
        <property type="nucleotide sequence ID" value="XM_014897536.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H630"/>
    <dbReference type="STRING" id="15368.A0A0Q3H630"/>
    <dbReference type="EnsemblPlants" id="KQK18408">
        <property type="protein sequence ID" value="KQK18408"/>
        <property type="gene ID" value="BRADI_1g42320v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100831112"/>
    <dbReference type="Gramene" id="KQK18408">
        <property type="protein sequence ID" value="KQK18408"/>
        <property type="gene ID" value="BRADI_1g42320v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20507at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H630">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000012">
        <property type="entry name" value="G patch domain-containing protein TGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011666">
        <property type="entry name" value="DUF1604"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13384">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13384:SF19">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07713">
        <property type="entry name" value="DUF1604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26093">
        <property type="entry name" value="HTH_TGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="SURP motif" evidence="11">
        <location>
            <begin position="412"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="468"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="684"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="831"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="468"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="693"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="850"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="871"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="887"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="912"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="942"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="954"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="981"/>
            <end position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KQK18408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="996" mass="112424" checksum="266C52204F4FDB60" modified="2016-01-20" version="1">MAFDDDDQDLVVYGTPIEREEDISARKRRAVADAGQLRALPAWKQEVRDEEGRRRFHGAFTGGFSAGFYNTAGSKEGWTPQTFTSSRKSRAEHKKQSIYNFLDEEDIKDMGGNALETSQQYDTFGFTAAEHARKQASKEQSERPSAIPGPVPDELVVPGTTSIGVKLLMKMGWRQGRTIKDAHADSLYESRREARKAFLALSGISNVEDQDQIASHTSRMDENVAESFDKIRASGNTPVYVLHPKEDLHGLGFDPFKHAPEFKDRKRLQKSLNRDGNRSDISMRGNLLLSNSGQYAPGFGIGALEELDVEDEDIYASVFSGFSYEQTEVDIEPSKTIGDSKFKLEDRRRGVFLTFKMALNSEYKLERFHPPEIPADFDGHHKFSSPIQAADKFSDLAPPEVPPPEDATLRLLVEGCAAMVARCGKRIEDFYKEKSKASPQFLFLDGGDGCGYYTRKLWEHQQKFIDQQKPDIVKSKPSSEKLTAENRGKILGERPLDRSSKSSSSSFSAKDAVQLQSNLGDTFVKPISLDAVPESKKPFRNEPAKQARFEQFLKEKYQGGLRLANLVPTSTMSEADRARERLDFEAAADAIEKGKEYKAIDPFSILGLPGLNEQRFVSSTQLESSVVPRDEKPAYPQREEFEWRPSPILCKRFDIVDPFMGKPMPLRRPISKMDTLMFMTESTKKTNDDVDSSSRTLQHASVSGTEETEAQGTADNPDIVSSSMQRPVDLYKAIFSDDSDDDMDEPLNNQPVDPVKTSEGANMALNRLVAEDFLESLGKELGLDVPPEKPALPANALFRSEILPMADRTVSSRNGKTTICRELKENESNLGLVEAANAQEDGPSSNVETLDLKCEKQEHRAEEGRSHSSHHQIQNCSPDSDTSVERHRSRKRRSHHRIQSEMSDSDSSSERRRSKKRKSRSRHRTGRIRTPDADSSCDSQKNKRKRQEKRSHRTRDTDSSDHEYKERYTSSSRRSSDKDRSRKRSRHRSHRRKDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3H7Q6</accession>
    <name>A0A0Q3H7Q6_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BRADI_1g43876v3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK18653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3H7Q6"/>
    <dbReference type="STRING" id="15368.A0A0Q3H7Q6"/>
    <dbReference type="EnsemblPlants" id="KQK18653">
        <property type="protein sequence ID" value="KQK18653"/>
        <property type="gene ID" value="BRADI_1g43876v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18653">
        <property type="protein sequence ID" value="KQK18653"/>
        <property type="gene ID" value="BRADI_1g43876v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3H7Q6"/>
    <dbReference type="OrthoDB" id="1898734at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3H7Q6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF2">
        <property type="entry name" value="PROLYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KQK18653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblPlants" id="KQK18653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="205" mass="22293" checksum="502DCDFBE89CB981" modified="2016-01-20" version="1">MAVRSSLRCATAAAALALHHQQPAFLAAVRLRLRRIPTRRAMSSSSSAAAAAAPHQAGTWHAVPDLSLRDHRFAVPLDHSAGSTPTITVFAREVVAAGKEEAPLPYLMYLQGGPGFESPRPMEASGWVKKACEEYRVVLLDQRGTGLSTPLTTSSLSQITSAVEQVEYLKHFRADNIVKDAEFIRLNLVPDAKPWTILGQVQYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HAB3</accession>
    <name>A0A0Q3HAB3_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_1g48813v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK19525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HAB3"/>
    <dbReference type="EnsemblPlants" id="KQK19525">
        <property type="protein sequence ID" value="KQK19525"/>
        <property type="gene ID" value="BRADI_1g48813v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK19525">
        <property type="protein sequence ID" value="KQK19525"/>
        <property type="gene ID" value="BRADI_1g48813v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3HAB3"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK19525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK19525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="139" mass="13742" checksum="2D836429B2BB888F" modified="2016-01-20" version="1" fragment="single">TGEGVHLLCEGKGYLACVKEPKGGGDGEGRGRGAGSGQRPRRRDGGGRGEERGRGAGTGETGGGGGGPPHRDGGARGWGRRGHAGGGGSGQRPRRRDRGGRGVGGAADRESDEGERLGEGDGGERLGEGDGVVVSTAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3HNM9</accession>
    <name>A0A0Q3HNM9_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100838099</name>
        <name evidence="15" type="ORF">BRADI_3g14500v3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Bd21</strain>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Bd21</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class IV subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="KQJ94986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000882">
        <property type="protein sequence ID" value="PNT66595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_010234287.2">
        <property type="nucleotide sequence ID" value="XM_010235985.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757112.1">
        <property type="nucleotide sequence ID" value="XM_014901626.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014757114.1">
        <property type="nucleotide sequence ID" value="XM_014901628.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3HNM9"/>
    <dbReference type="STRING" id="15368.A0A0Q3HNM9"/>
    <dbReference type="EnsemblPlants" id="KQJ94986">
        <property type="protein sequence ID" value="KQJ94986"/>
        <property type="gene ID" value="BRADI_3g14500v3"/>
    </dbReference>
    <dbReference type="EnsemblPlants" id="PNT66595">
        <property type="protein sequence ID" value="PNT66595"/>
        <property type="gene ID" value="BRADI_3g14500v3"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3I7H5</accession>
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            <strain evidence="2 3">Bd21</strain>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Bd21</strain>
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    </reference>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="113" mass="13555" checksum="614D33532E3268D1" modified="2016-01-20" version="1">MHRNSQENTCMVSFFSFYNIQIELLCFTVHYCFMWISNPFMFGLWIERCDCILWYGNGLISYFTFVESLSFSDHTPVYSIFMAEVEIVHQRKRDMGYFSSRIEVEKLLPHSES</sequence>
</entry>
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    <accession>A0A0Q3I7L5</accession>
    <name>A0A0Q3I7L5_BRADI</name>
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        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">BRADI_3g23240v3</name>
    </gene>
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            <taxon>Poaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
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            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="KQJ96467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_3g23240v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 3"/>
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        <property type="expression patterns" value="baseline"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:microtubule cytoskeleton organization"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="probable calcium-binding protein CML20"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
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        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="13"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KQJ96467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblPlants" id="KQJ96467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="183" mass="20887" checksum="1DED172241E37A12" modified="2016-01-20" version="1">MRERGRRKRLTAQKRKEIKEAFDLFDTDGSGTIDARELNVAMRALGFEMTPEQIRQMIAEVDKDGSGTIDLDEFVHMMTDKIGERDARDELTKAFRIIDQDGNGKISDVDIQRLAIDAGEHFTLDEVREMIEAADEDGTYPFLFFSSDRPCIALAVLLLNMMWLFKSLTLSTQVTVRSTWTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3IWR3</accession>
    <name>A0A0Q3IWR3_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vacuolar protein sorting-associated protein 28 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106865427</name>
        <name evidence="8" type="ORF">BRADI_2g51800v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Bd21</strain>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the VPS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK10075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="15368.A0A0Q3IWR3"/>
    <dbReference type="EnsemblPlants" id="KQK10075">
        <property type="protein sequence ID" value="KQK10075"/>
        <property type="gene ID" value="BRADI_2g51800v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK10075">
        <property type="protein sequence ID" value="KQK10075"/>
        <property type="gene ID" value="BRADI_2g51800v3"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2671at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3IWR3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Vacuolar protein sorting-associated protein 28 homolog"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Vps28"/>
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        <property type="entry name" value="VPS28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037206">
        <property type="entry name" value="VPS28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017898">
        <property type="entry name" value="VPS28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038358">
        <property type="entry name" value="VPS28_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 28, ISOFORM 2 VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS28 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="VPS28 C-terminal" evidence="6">
        <location>
            <begin position="112"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KQK10075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK10075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="29037" checksum="3E1CEC5ABDF7652D" modified="2016-01-20" version="1">MEVKLWNDKRERELLESLADLYAIIKATEKLERAYVRDLVSAADYEAECLKLISQFNSLSSSLAGVVTIPRFVQAYRLDCPAALNRLVQSGVPATVELRASTNSSAPAATAASAAAIAQCVQSFITAMDAVKLNMLANDQVRPLLHDLSTSMGKLGTVLPPDFEGKVKVNEWLAKLHKMGAGDELTEQQVVWCYQFFGLLQSKSKEINDRRYSCNAGREERGRQRRLGRGRSGSGARIAAVARTLATRGSQACERAASMSSIDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3J7W0</accession>
    <name>A0A0Q3J7W0_BRADI</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BRADI_2g43052v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK08604.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3J7W0"/>
    <dbReference type="EnsemblPlants" id="KQK08604">
        <property type="protein sequence ID" value="KQK08604"/>
        <property type="gene ID" value="BRADI_2g43052v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK08604">
        <property type="protein sequence ID" value="KQK08604"/>
        <property type="gene ID" value="BRADI_2g43052v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3J7W0"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994:SF29">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN LEA-2 SUBGROUP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33994">
        <property type="entry name" value="OS04G0515000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK08604.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK08604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="161" mass="17853" checksum="7443B08B756D031E" modified="2018-03-28" version="2">MPLPSAPSPRCAPRRTGPWAPCRSRRSSTPPSRPCLGHSTCAFDGFWRGTSGDRCRRPDGPIAQPGDPPSFNLTLRGESDVHDLPQDCWKMVRVTVFYGSAILGWGSVPNFCMARHGWVEVNAAMSHTDVMLTDELRRSMASELNSGELEFQICISESYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JE16</accession>
    <name>A0A0Q3JE16_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Patatin</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BRADI_1g27430v3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Bd21</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Bd21</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">cv. Bd21</strain>
        </source>
    </reference>
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        <text evidence="6">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="function">
        <text evidence="4">Possesses non-specific lipolytic acyl hydrolase (LAH) activity. Hydrolyzes phospholipids as well as galactolipids. May play a role in disease resistance.</text>
    </comment>
    <comment type="domain">
        <text evidence="6">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the patatin family.</text>
    </comment>
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    <dbReference type="EMBL" id="CM000880">
        <property type="protein sequence ID" value="KQK16211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0A0Q3JE16"/>
    <dbReference type="EnsemblPlants" id="KQK16211">
        <property type="protein sequence ID" value="KQK16211"/>
        <property type="gene ID" value="BRADI_1g27430v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK16211">
        <property type="protein sequence ID" value="KQK16211"/>
        <property type="gene ID" value="BRADI_1g27430v3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="1658288at2759"/>
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        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
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        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF27">
        <property type="entry name" value="PATATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FabD/lysophospholipase-like"/>
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    </dbReference>
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        <property type="entry name" value="PNPLA"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 5 6" id="KW-0443">Lipid metabolism</keyword>
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    <feature type="domain" description="PNPLA" evidence="7">
        <location>
            <begin position="25"/>
            <end position="227"/>
        </location>
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    <feature type="short sequence motif" description="GXGXXG" evidence="5">
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            <begin position="29"/>
            <end position="34"/>
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        <location>
            <position position="60"/>
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    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
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            <position position="214"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KQK16211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblPlants" id="KQK16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="366" mass="40121" checksum="47916D2B49C4A33B" modified="2016-01-20" version="1">MEGGKCNPATGPCPPPSQGRLITVLSIDGCGIRGLIPSTIIDYGPEARIADYFDVISGTSTGALVTSMLAAPGPNKRPLFAASKLKDFYLENGPKIFPRKKLGFLNPAANMFGAVMGPKYDGKFLHDKIKELTHKAKIKDMITNIIVPTFDVNTLQPIIFSTYEAKEDSLKNAHLSDICISTSAAPTYFPAHLFKTYDSDPKAKVKEWEYHLVDGGVAANNPTMVAMTMITKEDDDHQGGSAKRKYPAPKCAKWGVLRWLYDDGFTPLIDIFSHASADMVDIHAAVLFKALCVKENYLRIQHDSLEGDTSSVDIATKKNMHVLIGIGKELLERKVARVNINTGVYETMEGDGTKHPFKKGFLIVTI</sequence>
</entry>
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    <accession>A0A0Q3JI45</accession>
    <name>A0A0Q3JI45_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">BRADI_1g34225v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
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            <taxon>Poaceae</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            <strain evidence="2 3">Bd21</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3JI45"/>
    <dbReference type="EnsemblPlants" id="KQK17403">
        <property type="protein sequence ID" value="KQK17403"/>
        <property type="gene ID" value="BRADI_1g34225v3"/>
    </dbReference>
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        <property type="gene ID" value="BRADI_1g34225v3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="245"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KQK17403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblPlants" id="KQK17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
        </source>
    </evidence>
    <sequence length="386" mass="42172" checksum="F9BFDF0EE3FDDE33" modified="2016-01-20" version="1">MVFRHVAPTSQAPRQRQDPPFRHPAVINHPRAASQHLPSCLKKEAATAGDLRRRPMVLLHPRTCSNERPELLLVSQPRHAPSLPRVGFLSRLRRSSTAFASSPPVAPVSHSRAREHCATSLSLQADHRRLPPPHRRGTTLAPDSPHRRLPFHRDGAHTKGSLVRLAVHRGAAVAAIFSAPGDGLPRSRGMLWPRDGECDGGVPDGLVLVMMWREIAATLPISLSHPNTLLSPKLLLSASPAPVPPLAPPSLEDEGHSGRPGPRRLRIRPSLPPPSPDPASLTRPPRPPPPPDPASLGPPVANPVRDPPESRRHRRRTRLRRAVQHRCMVQDELTGGVDGSVRRRVSAHPSRIRTASGFGLQCGPGGHFNFFFCKNISLSGVVCDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JLL4</accession>
    <name>A0A0Q3JLL4_BRADI</name>
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        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BRADI_1g43260v3</name>
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    <organism evidence="4">
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        <name type="synonym">Trachynia distachya</name>
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            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
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            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Bd21</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
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                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Bd21</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">cv. Bd21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="EnsemblPlants" id="KQK18555">
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        <property type="gene ID" value="BRADI_1g43260v3"/>
    </dbReference>
    <dbReference type="Gramene" id="KQK18555">
        <property type="protein sequence ID" value="KQK18555"/>
        <property type="gene ID" value="BRADI_1g43260v3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0Q3JLL4"/>
    <dbReference type="OrthoDB" id="653237at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3JLL4">
        <property type="expression patterns" value="baseline and differential"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </dbReference>
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        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF394">
        <property type="entry name" value="OS06G0297400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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            <end position="64"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="2">
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        </location>
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        </location>
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    <sequence length="949" mass="105031" checksum="845250DF0386E33A" modified="2016-01-20" version="1">MVDSEEQNGNGREPAANTRDAPNGQQEGPNPGVGDEAVLLWKLRKYLVLLAILAAAITYQAGLAPPGGFWQETKDGRVASDIVLRASYPRRYLVFFYCNTTAFGASLMVLILLLVRELSRNAIWLRSLQFAMILGLLGLMGAYAAGSCREVRTSVYIWVLLVGIFAYVMLHVLFFLHLAPEGLLAIFSDVRRSWERTLENIFRKPEGTERDRIAPAQEAADQKEELERNRSSLLVLATLAATVTYVAGLSPPGGFWPDNNGIHLAGDPVLRDHYPRRFKAFLVCNATAFAGSLVIIIMLLSAAVDQVVKSNALRFCVLVSLFGLMGAYAAGSCREVQTSIYVFALVGAVLLYLLIQFIVPVMCLKAKKDEMLEKLNAFIMTPRALTPPSEHDLPHQSNNNTPDTDDNVKHDLQKLRTYLLLLGILAATVTYQAGLNPPGGFWTDNSDGHIAGDPILEVLSPKRYKAFFYCNATAFVASLVIIILLQSQSITIHAMKRHVLQSAMSLVLFGLMGAYAAGSSRKFSTSIYVFVLILLVFAYVVLHAVLYVALKTRLKSIIGKIEAYTSKLLHCFGDSSESGDAVLEEKDLQKRRKFLMLLAILAASITYQAGISPPGGFWTDNKNGHRAGYSVFRDEFRNRYRVFFYFNATAFMASLAVILLLVSKRLCHKGLKCYALRACVLVDLISLMGAFAAGSCRKVSTSVYVILVVVAVFVYVMIQVLVLTFAKEKVNGLVKWVLELLKAKAFELQRHKCEDQSMNHTTSPPRNERENKWRKDLMLIATLAVTVTYQAGLLPPGGVWPDDQYGHFAGDPILHDTNLKRYKVFFYCNATAFMASMVMVILLLNNTISKYKRSLLAMKMAMVFVLLGLLGAYAAGSCRKLKTSAYIFALVIAVIIYIAVHVLLSFDKVDKLMKEKREKWMPCLNTSAPIGVDSNNQPSAGESGVSLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-03-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3JXR5</accession>
    <name>A0A0Q3JXR5_BRADI</name>
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        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100822525</name>
        <name evidence="2" type="ORF">BRADI_2g06710v3</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
            <authorList>
                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Bd21</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Bd21</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">cv. Bd21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CM000881">
        <property type="protein sequence ID" value="KQK03265.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003565437.4"/>
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            <dbReference type="EMBL" id="KQK03265.2"/>
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            <dbReference type="EnsemblPlants" id="KQK03265"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008810"/>
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    <sequence length="286" mass="30645" checksum="51C54BBC265DB68D" modified="2018-03-28" version="1">MESSLCTPSHAMATRAHAAPSTASPRRASTSPSLSLLQPGRLRARAVRAHSRAPEPEPEPQAALPRTTALRVGAGVALALALGSASWAAPPWRGGGGAGTALVQPAALMCTLNAVADGAERGGAGIPAAVPGVRTSVDALSDSLFRREDAPRDRATLTDLVFEQVTKEHIGDRGKLTSLLQKEWAASRDSERKLDLGLLLTDVLVNQREWQRAKEVCQQLTGRYQRDSRPYLHLAVINMMMAVETMLSPETATADEIEKMSKNAMDAWKEFKNKYELAKGATESSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0Q3L477</accession>
    <name>A0A0Q3L477_BRADI</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Morc S5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="18" type="primary">LOC100843690</name>
        <name evidence="17" type="ORF">BRADI_4g10980v3</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Brachypodium distachyon</name>
        <name type="common">Purple false brome</name>
        <name type="synonym">Trachynia distachya</name>
        <dbReference type="NCBI Taxonomy" id="15368"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>Liliopsida</taxon>
            <taxon>Poales</taxon>
            <taxon>Poaceae</taxon>
            <taxon>BOP clade</taxon>
            <taxon>Pooideae</taxon>
            <taxon>Stipodae</taxon>
            <taxon>Brachypodieae</taxon>
            <taxon>Brachypodium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="463" first="763" last="768">
            <title>Genome sequencing and analysis of the model grass Brachypodium distachyon.</title>
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                <consortium name="International Brachypodium Initiative"/>
            </authorList>
            <dbReference type="PubMed" id="20148030"/>
            <dbReference type="DOI" id="10.1038/nature08747"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Bd21</strain>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2017-06" db="EMBL/GenBank/DDBJ databases">
            <title>WGS assembly of Brachypodium distachyon.</title>
            <authorList>
                <consortium name="The International Brachypodium Initiative"/>
                <person name="Lucas S."/>
                <person name="Harmon-Smith M."/>
                <person name="Lail K."/>
                <person name="Tice H."/>
                <person name="Grimwood J."/>
                <person name="Bruce D."/>
                <person name="Barry K."/>
                <person name="Shu S."/>
                <person name="Lindquist E."/>
                <person name="Wang M."/>
                <person name="Pitluck S."/>
                <person name="Vogel J.P."/>
                <person name="Garvin D.F."/>
                <person name="Mockler T.C."/>
                <person name="Schmutz J."/>
                <person name="Rokhsar D."/>
                <person name="Bevan M.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Bd21</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2018-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblPlants"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">cv. Bd21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MORC ATPase protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000883">
        <property type="protein sequence ID" value="KQJ87413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_010239151.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0Q3L477"/>
    <dbReference type="EnsemblPlants" id="KQJ87413">
        <property type="protein sequence ID" value="KQJ87413"/>
        <property type="gene ID" value="BRADI_4g10980v3"/>
    </dbReference>
    <dbReference type="GeneID" id="100843690"/>
    <dbReference type="Gramene" id="KQJ87413">
        <property type="protein sequence ID" value="KQJ87413"/>
        <property type="gene ID" value="BRADI_4g10980v3"/>
    </dbReference>
    <dbReference type="KEGG" id="bdi:100843690"/>
    <dbReference type="OrthoDB" id="757982at2759"/>
    <dbReference type="Proteomes" id="UP000008810">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0Q3L477">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
   
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